CN105076138B - An insecticidal composition of chlorfenapyr and pyriproxyfen, its preparation and application - Google Patents

Abstract

The invention discloses a kind of capillary and the Pesticidal combination and its preparation of Nylar and application.The Pesticidal combination is (0.1~10) by mass ratio：1 capillary and Nylar composition.Preferably, the mass ratio of the capillary and Nylar is (0.1~0.25)：1 or (4~10)：1, but be not 0.25：1 and 4：1.The insecticide as made from the Pesticidal combination, its acceptable auxiliary material on above-mentioned Pesticidal combination and its pesticidal preparations is made, wherein, the weight/mass percentage composition of Pesticidal combination is 2%~60%.The present invention has obvious synergistic effect to Bradysia odoriphaga by after capillary and Nylar according to a certain percentage mixture；Because capillary and two kinds of pharmacy effect mechanism of Nylar are different, the application of composition helps to delay the development of pest resistance to insecticide, extends the service life of medicament；The insecticide that the present invention is made up of the Pesticidal combination after capillary and Nylar mixture, has preferable prevention effect to Bradysia odoriphaga.

Description

An insecticidal composition of chlorfenapyr and pyriproxyfen, its preparation and application

technical field

The invention relates to an insecticidal composition of chlorfenapyr and pyriproxyfen, its formulation and application, and belongs to the technical field of pesticides.

Background technique

Chlorfenapyr (Chlorfenapyr), the chemical name is 4-bromo-2-(4-chlorophenyl)-1-ethoxymethyl-5-trifluoromethylpyrrole-3-carbonitrile, which is produced by American cyanamide company In the late 1980s, an arylpyrrole insecticide and acaricide was successfully developed by modifying the chemical structure of natural antibiotics. It is an inhibitor of mitochondrial oxidative phosphorylation in insects.

Pyriproxyfen (Pyriproxyfen), the chemical name is 4-phenoxyphenyl (RS)-2-(2-pyridyloxy) propyl ether, is an insect growth regulator that disrupts the growth of insects with phenyl ethers, and belongs to juvenile hormone A new type of insecticide similar to it has the characteristics of low toxicity and long-lasting effect, which is safe to crops, low toxicity to fish, and has little impact on the ecological environment.

The use of single-dose pesticides is often restricted by factors such as control effect, application range, and phytotoxicity, and with the increase of resistance to pests and diseases, the control effect decreases. Pesticide compounding can overcome the shortcomings of the original pesticide single agent. For insecticides, the commonly used compounding methods are: organophosphorus and organophosphorus, organophosphorus and pyrethroids, organophosphorus and carbamate Classes, organic nitrogen and carbamates, organic nitrogen and pyrethroids, etc.

Bradysia odoriphaga Yang et Zhang, commonly known as chive maggots, belongs to Diptera, Longocera, Oculophagoidae, and Bradysia odoriphaga. It is a characteristic vegetable in northern my country that damages leeks, garlic, and onions. Among them, leeks are the most serious pests, which is one of the main reasons for the current production reduction of leeks and excessive pesticide residues.

In recent years, due to the increase in the planting area of leeks in facilities, the harm of leek maggots has increased year after year. The continuous use of conventional insecticides for many years has led to increased resistance of pests and unsatisfactory control effects. In some places, vegetable farmers have used highly toxic insecticides in violation of regulations in order to maintain production, frequently causing food safety problems. Therefore, it is very necessary to screen high-efficiency, low-toxicity, safe and environment-friendly agents.

Contents of the invention

The object of the present invention is to provide an insecticidal composition of chlorfenapyr and pyriproxyfen, its preparation and application. In the present invention, by compounding chlorfenapyr and pyriproxyfen, the insecticidal composition prepared has obvious synergistic effect on tardy-eyed fungi, and is a high-efficiency, low-toxicity, low-residue pesticide composition; The insecticidal composition is made into various formulations of insecticides, all of which have good control effects on tardy-eyed fungus chives.

The insecticidal composition provided by the invention is composed of chlorfenapyr and pyriproxyfen in a mass ratio of (0.1-10):1.

In the above-mentioned insecticidal composition, the control objects of chlorfenapyr mainly include vegetable pests such as diamondback moth, cabbage caterpillar, beet armyworm, litura litura, cabbage borer, cabbage aphid, liriomyza and thrips.

In the above-mentioned insecticidal composition, the control objects of pyriproxyfen mainly include Homoptera (bemisia tabaci, greenhouse whitefly, green peach aphid, scorpion, cotton scale and red wax scale, etc.), Thysanoptera (palm Thrips), Lepidoptera (Plutella xylostella), Cycloptera (book lice), Blattata (German cockroach), Pleasia (fleas), Coleoptera (Heterochromatia), Neuroptera ( Chinese lacewing) and other insects have special effects on whiteflies, scale insects and cockroaches.

In the present invention, chlorfenapyr and pyriproxyfen are compounded according to a certain ratio, and through a large number of bioassay screenings, it is found that the insecticidal composition of the present invention exhibits obvious synergistic effect on T. tardigrades. With reference to Sun Yunpei (Sun YP) method, evaluate the interaction between two different active components in the present invention after mixing, wherein, the co-toxicity coefficient is greater than 120 for synergism, and the co-toxicity coefficient is less than 80 for antagonism, and the co-toxicity coefficient is greater than 120 for antagonism, and the co-toxicity coefficient is greater than 120 for antagonism. There is an additive effect between them. By calculating the co-toxicity coefficient of chlorfenapyr and pyriproxyfen after mixed use, the effect after the evaluation of the two mixed, the results show that in the above insecticidal composition, the mass ratio of chlorfenapyr and pyriproxyfen can be (0.1～ 10): 1, specifically (0.1~5): 1, (0.1~1): 1, (0.1~0.2): 1, (0.2~10): 1, (0.2~5): 1, (0.2 ~1):1, (1~10):1, (1~5):1, (5~10):1, 0.1:1, 0.2:1, 1:1, 5:1 or 10:1, Within the range of the above-mentioned mass ratio, all exhibit synergistic effect.

In the above insecticidal composition, the mass ratio of chlorfenapyr and pyriproxyfen is preferably (0.1-0.25):1 or (4-10):1, but not 0.25:1 and 4:1; more Preferably (0.2-0.25):1 or (4-5):1, but not 0.25:1 and 4:1; more preferably 0.2:1 or 5:1.

Due to the synergistic effect of the above-mentioned chlorfenapyr and pyriproxyfen after compounding according to the above-mentioned ratio, the insecticidal composition composed of the above-mentioned chlorfenapyr and pyriproxyfen in any mass ratio provided by the present invention is effective in the prevention and treatment of tardigrade maggots. The application is also within the protection scope of the present invention.

Since the original drug cannot be used directly, the present invention further provides a kind of insecticide, which is made from the above-mentioned insecticidal composition and its acceptable auxiliary materials for pesticide preparations, wherein the quality of the insecticidal composition The percentage content can be 2% to 60%, specifically 6% to 24%, 6% to 20%, 6% to 12%, 12% to 24%, 12% to 20%, 20% to 24%, 6%, 12%, 20% or 24%.

Among the above-mentioned insecticides, the mass percentage of the insecticidal composition is preferably 10%-60%, more preferably 12%-24%, even more preferably 12%-20% or 20%-24%.

During the implementation of the present invention, since the content of active ingredients in the original drug cannot be 100%, such as 95% chlorfenapyr original drug means that the mass percentage of chlorfenapyr in the original drug is 95%. In the calculation process of the inventive insecticidal composition and insecticide, it has been converted into the amount of active ingredients, that is, the content of the original drug in the insecticidal composition and insecticide refers to the content of the active ingredients.

Among the above-mentioned insecticides, the insecticides can be processed into preparations in suitable dosage forms by any currently known method, and suitable dosage forms include but are not limited to: suspensions, suspension seed coatings, suspoemulsions, emulsifiable concentrates, emulsions in water , microemulsion, water dispersible granule, wettable powder, granule, pouring agent, drop, microcapsule suspension, microcapsule suspension-suspension agent, etc., preferably suspension formulation; commonly used additives are wetting agent, dispersing One or several of the agents, solvents, release agents, stabilizers, antifreeze agents, preservatives, fillers, adhesives, etc., are known substances, and are commonly used auxiliaries in pesticide adjuvants. Something has changed.

The application of the above-mentioned insecticides in the prevention and treatment of the tardigrade leek is also within the protection scope of the present invention, and the application results show that after 21 days and 35 days of application, compared with a single dose, the insecticides of the present invention It has obvious control effect on tardigrades of chives; the application rate of the total active ingredients (insecticidal composition) in the insecticide is 200-600 g/hm ² , specifically 200-400 g/hm ² , 400 g/hm 2 ~600 g/hm ² , 200 g/hm ² , 400 g/hm ² or 600 g/hm ² , preferably 400 to 600 g/hm ² , more preferably 600 g/hm ² .

The present invention has following beneficial effect:

(1) In the present invention, after mixing chlorfenapyr and pyriproxyfen according to a certain ratio, it has obvious synergistic effect on T. tardigrades.

(2) Since the action mechanisms of chlorfenapyr and pyriproxyfen are different from each other, the application of the composition helps to delay the development of pest resistance and prolong the service life of the agents.

(3) The pesticide preparation made from the insecticidal composition mixed with chlorfenapyr and pyriproxyfen has good control effect on tardy-eyed fungus.

detailed description

The experimental methods used in the following examples are conventional methods unless otherwise specified.

The materials and reagents used in the following examples can be obtained from commercial sources unless otherwise specified.

After different pesticides are mixed, they usually show three types of effects, namely additive effect, synergistic effect and antagonistic effect, but the specific effect can only be known through a large number of experiments. A good compound and synergistic formula can significantly improve the actual control effect, expand the insecticidal spectrum of the pesticide, and help to delay the generation of pest resistance.

In the following example 1, through a large number of screening tests, chlorfenapyr and pyriproxyfen were compounded according to different ratios, and it was found that the compounding of chlorfenapyr and pyriproxyfen had a significant synergistic effect on the tardigrade leek. effect.

Embodiment 1, insecticidal composition and compound effect thereof

In this example, chlorfenapyr and pyriproxyfen were compounded according to the mass ratio of 10:1, 5:1, 1:1, 1:5 or 1:10, respectively, and the effects on chives and tardigrade were tested respectively. Combined virulence of the second and fourth instar larvae of the fungus mosquito.

Test object: the second and fourth instar larvae of T. chinensis.

Test agents: 95% chlorfenapyr and 97% pyriproxyfen. The original drug (after 100% discount) is prepared into the required test drug according to the conventional method.

Experimental method: Indoor bioassay was carried out by stomach poison contact combined with toxicity method, and the second and fourth instar larvae of T. tardiformis were selected as the target pests respectively, and 25 larvae were treated at each concentration, and each treatment was repeated 4 times. Place them in a constant temperature incubator at 25±1°C for observation, and keep the surviving larvae until they pupate and emerge, and record the final number of adults that emerge normally. The probability analysis method and DPSv7.5 software were used to process the data, and the lethal intermediate concentration LC ₅₀ was calculated, and the co-toxicity coefficient was calculated according to Sun Yunpei's method with the LC ₅₀ value, and the calculation formula was shown in formula (1).

Formula 1)

Let the mixed medicine be M, and the individual medicines be A, B, C.... LC _50A is the lethal intermediate concentration of agent A; LC _50B is the lethal intermediate concentration of agent B; LC _{50 is the lethal intermediate concentration of the mixture} of agent A+agent B+agent C+..., the co-toxicity coefficient of the mixture is CTC, the active ingredient The percentage content is P.

A co-toxicity coefficient (CTC) ≥ 120 represents a synergistic effect, a CTC ≤ 80 represents an antagonistic effect, and 80 < CTC < 120 represents an additive effect.

Table 1 shows the results of the joint toxicity test of the insecticidal composition of the present invention to the second and fourth instar larvae of T. chinensis.

Table 1. The combined toxicity of chlorfenapyr and pyriproxyfen to the second and fourth instar larvae of T.

It can be seen from Table 1 that in the insecticidal composition of the present invention, when the mass ratio of chlorfenapyr to pyriproxyfen is 10:1, 5:1, 1:1, 1:5 and 1:10, all of them show an increase. In terms of efficacy, the co-toxicity coefficients of 5:1, 1:1 and 1:5 are relatively large. When the ratio is 5:1, the co-toxicity coefficient is the highest, that is, the synergistic effect is the most obvious.

Following embodiment 2-embodiment 7 is the various preparations that insecticidal composition of the present invention is made respectively, can be prepared into any dosage form suitable for agricultural use by known method, and better dosage form is suspension concentrate, Suspension seed coating, suspoemulsion, emulsifiable concentrate, water emulsion, microemulsion, water dispersible granule, wettable powder, granule, pouring agent, drop, microcapsule suspension, microcapsule suspension-suspension concentrate.

In order to clarify the purpose, technical solutions and advantages of the present invention, the present invention is illustrated with the following specific examples, but the active ingredients, their proportions, and auxiliary agents are not limited to the following examples.

Example 2, 10% chlorfenapyr + 2% pyriproxyfen suspending agent

1. Formula

In terms of mass percentage, the suspending agent of this embodiment consists of the following components:

2. Preparation method

Prepare the suspension as follows:

Mix active components, dispersant, wetting agent, water and other components according to the ratio of the formula, shear at 10000r/min for 5 minutes to be uniform, add sand mill to grind to the required particle size (average particle size is less than 3 microns), made into 10% chlorfenapyr + 2% pyriproxyfen suspension concentrate.

Example 3, 10% chlorfenapyr + 10% pyriproxyfen suspending agent

1. Formula

In terms of mass percentage, the suspending agent of this embodiment consists of the following components:

2. Preparation method

Prepare the suspension as follows:

Mix active components, dispersant, wetting agent, water and other components according to the ratio of the formula, shear at 10000r/min for 5 minutes to be uniform, add sand mill to grind to the required particle size (average particle size is less than 3 microns), made into 10% chlorfenapyr + 10% pyriproxyfen suspension concentrate.

Example 4, 2% chlorfenapyr + 10% pyriproxyfen suspending agent

1. Formula

In terms of mass percentage, the suspending agent of this embodiment consists of the following components:

2. Preparation method

Prepare the suspension as follows:

Mix active components, dispersant, wetting agent, water and other components according to the ratio of the formula, shear at 10000r/min for 5 minutes to be uniform, add sand mill to grind to the required particle size (average particle size is less than 3 microns), made into 2% chlorfenapyr + 10% pyriproxyfen suspension concentrate.

Example 5, 5% chlorfenapyr + 1% pyriproxyfen granules

1. Formula

In terms of mass percentage, the granules of this embodiment consist of the following components:

2. Preparation method

Prepare granules as follows:

Mix the above components evenly to make a soft material. The prepared soft material is granulated by an extrusion granulator and dried to make 5% chlorfenapyr + 1% pyriproxyfen granules.

Example 6, 10% chlorfenapyr + 2% pyriproxyfen wettable powder

1. Formula

In terms of mass percentage, the wettable powder of this embodiment consists of the following components:

2. Preparation method

Prepare wettable powder according to the following steps:

The above-mentioned components are mixed uniformly, crushed by airflow, and then fully mixed to prepare 10% chlorfenapyr + 2% pyriproxyfen wettable powder.

Example 7, 10% chlorfenapyr + 2% pyriproxyfen microcapsule suspension-suspension agent

The present embodiment prepares the microcapsule suspension according to the following method, and the specific steps are as follows:

Add 4.12g of 97% pyriproxyfen technical substance into 10g of xylene to fully dissolve, then add 3g of isocyanate and 1.5g of PEG 600, stir well, and use it as the oil phase; 0.2g of tristyrylphenol polyoxyethylene ether, 3g of lignin Calcium sulfonate and 0.1g polyvinyl alcohol are uniformly mixed with 53.54g water as the water phase; the oil phase is mixed with the water phase, and sheared and emulsified with a high-speed homogenizer to obtain a stable O/W emulsion; the O/W emulsion Transfer to a flask, stir at a speed of 200 rpm, heat up to 65°C, react for 4 hours, adjust the pH value to 6-6.5 with citric acid; add 2g of ethylene glycol, 0.14g of xanthan gum, stir well, and make up 100g of water , to obtain 4% pyriproxyfen microcapsule suspension.

Weigh 21.05g 95% chlorfenapyr technical, 5g styrene phenol formaldehyde resin polyoxyethylene polyoxypropylene ether, 3g calcium lignosulfonate, 1.5g nonylphenol polyoxyethylene ether, 0.12g xanthan gum, 3g Ethylene glycol, 0.5g of silicone defoamer, and 100g of water. Weigh each component according to the above formula and place it in a beaker, turn on the high-speed shearing machine for 5 minutes, and then grind it with a sand mill at 1500 rpm. More than 95% of the sample particle size is 1-5 microns, and the average particle size is 3 Micron below, get 20% chlorfenapyr suspension concentrate.

Mix 4% pyriproxyfen microcapsule suspension concentrate and 20% chlorfenapyr suspension concentrate at a weight ratio of 1:1 to obtain 2% pyriproxyfen + 10% chlorfenapyr microcapsule suspension-suspension concentrate.

Embodiment 8, field efficacy test

The preparations of various dosage forms prepared in Examples 2-Example 7 were subjected to a field efficacy test for preventing and controlling T. tardigrades of Chinese chives, and their practical application effects were investigated.

1) Application method

Using root irrigation application technology, the preparation is initially diluted and applied evenly with water. The operation method is as follows: add water to the suspending agent, wettable powder and other dosage forms of the composition during the occurrence period of the tardigrade leek, and make a suspension with water to rinse the roots, or spray it on the roots of the leek and then water it for use; the combination The granules can be mixed with fine sand, sprinkled on the roots of leeks, and then watered.

2) Cell area and repetition (leek)

Residential area: 30m ² .

Number of repetitions: Repeat 4 times.

3) Survey method

Survey on the rate of damaged plants: Sampling at five "Z"-shaped points in each plot, and taking leeks with a row length of 1m at each point, and counting the number of damaged plants.

4) Investigation time and frequency:

The control effect was investigated 21 days and 35 days after spraying, and the investigation was conducted twice.

5) Calculation method:

Calculate the population reduction rate and corrected control effect of each treatment. Calculated as follows:

Decrease rate of insect population (%)=(number of insect population before control-number of insect population after control)/number of insect population before control×100(2)

Corrected control effect (%)=(the insect population reduction rate in the chemical treatment area-the insect population reduction rate in the control area)/(1-the insect population reduction rate in the control area)×100(3)

The experimental results are shown in Table 2.

Table 2 Statistical results of the field efficacy test of the mixture of chlorfenapyr and pyriproxyfen in the control of tardigrade gnats

The results in Table 2 show that when the pesticide dosage forms are all suspension concentrates, Example 2 (chlorfenapyr: pyriproxyfen = 5: 1), Example 3 (chlorfenapyr: pyriproxyfen = 1: 1) and Example 4 (chlorfenapyr: pyriproxyfen = 1:5) has higher control effects on Chive tardigrades than the two agents alone, wherein, in Example 2 (chlorfenapyr: pyriproxyfen = The effect of 5:1) is very obvious, which is consistent with the result that the insecticidal composition under this ratio has the best synergistic effect. When the ratio of chlorfenapyr and pyriproxyfen is both 5:1, but the formulations are different, it can be seen from the results that the suspension agent has the best control effect, followed by microcapsule suspension concentrate, wettable powder, and granule The prevention effect is poor.

Claims (4)

1. the application of a kind of insecticidal composition in the prevention and treatment of tardigrade leek, described insecticidal composition is made up of chlorfenapyr and pyriproxyfen; The mass ratio of described chlorfenapyr and pyriproxyfen is 5: 1. 2. the application of a kind of insecticide in the prevention and treatment of Bradyeyemia leek, described insecticide is made by the insecticidal composition described in claim 1 and the acceptable adjuvant of its pesticide preparation, wherein, said The mass percent content of the insecticidal composition is 2% to 60%. 3. The application according to claim 2, characterized in that: in the insecticide, the mass percentage of the insecticidal composition is 6%-24%. 4. according to the described application of claim 2 or 3, it is characterized in that: the dosage form of described insecticide is suspension concentrate, suspension seed coating, suspoemulsion, emulsifiable concentrate, water emulsion, microemulsion, water dispersible granule, Any one of wet powder, granule, pouring agent, drop, microcapsule suspension and microcapsule suspension-suspension concentrate.