CN105031620A - Avian reovirus inhibition method aiming at chick embryos - Google Patents
Avian reovirus inhibition method aiming at chick embryos Download PDFInfo
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- CN105031620A CN105031620A CN201510411853.9A CN201510411853A CN105031620A CN 105031620 A CN105031620 A CN 105031620A CN 201510411853 A CN201510411853 A CN 201510411853A CN 105031620 A CN105031620 A CN 105031620A
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- avian reovirus
- gallus domesticus
- embryo gallus
- chick
- alpha interferon
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Abstract
The invention provides an avian reovirus inhibition method aiming at chick embryos. According to the method, chick alpha interferon is injected into the chick embryos creatively, so that resistance, to the avian reovirus, of the chick embryos is improved remarkably, and proliferation of the avian reovirus is inhibited obviously. In a preferable technical scheme, preferably, best inhibition effect on the avian reovirus by injecting the chick alpha interferon is found under the condition that the chick embryos are 9-11 days old; preferably, dosage of 20-40ug can ensure both excellent virus inhibition effect and safety. In addition, the avian reovirus inhibition method can be used for virus inhibition of disease-infected chick embryos. The chick alpha interferon is ingeniously utilized to inhibit the avian reovirus, so that immunity of livestock and poultry can be enhanced on the basis that drug resistance is avoided. The avian reovirus inhibition method is outstanding in effect, low in cost and easy in realization, thereby having outstanding popularization prospect.
Description
Technical field
The present invention relates to livestock-raising technical field, be specifically related to a kind of Avianreovirus suppressing method for Embryo Gallus domesticus.
Background technology
Poultry egg source property disease refer to birds at embryo stage caused by infectious pathogenic microorganisms, can via the disease of germ cell vertical transmission.Avian viral arthritis is a kind of chicken infectious disease caused by Avianreovirus (Avianreovirus, ARV).Cardinal symptom is that disease chicken walks lamely, squats, is reluctant to walk about, and stndon sheath and the swelling of toe extensor tendon are bent in chordae tendineae enlargement above articulatio tibiotarsalis, toe, and the tendon rupture of severe patient calf abdominal muscle, can cause death.Because the sick chicken dyskinesia, growth retardation, mortality increase, make the chicken of forfeiture economic worth up to 30% ~ 50%.It is the disease of egg source property vertical transmission, and especially as ARV and other pathogen mixed infection, case fatality rate significantly raises, and causes serious harm and huge economic loss to poultry husbandry.
Infectious disease endangers the important diseases of China's poultry cultivation industry development at present, and especially the harm of viral disease is serious.In poultry disease prevention and control, the application of various medicine serves positive effect to the reduction generation of fowl diseases and the treatment of fowl diseases, but the exploitation of newtype drug relatively lags behind.In prior art, chicken alpha interferon, as important cytokine, has the effect such as antitumor and immunomodulating, is for the more satisfactory medicine of relevant disease.Prior art is thought, interferon is direct killing or suppression virus not, and mainly make cell produce antiviral protein by cell surface receptor effect, that is, the maturation immunity system that interferon relies on independent life body usually plays a role, not as antibiotic, independently has antibacterial properties, is therefore generally only limitted to the auxiliary of body's immunity to the application of interferon in prior art and regulates.
Summary of the invention
The present invention is intended to the technological deficiency for prior art, provides a kind of Avianreovirus suppressing method for Embryo Gallus domesticus, is difficult to carry out the technical problem of effectively suppression in the Embryo Gallus domesticus stage to Avianreovirus to solve in prior art.
For realizing above technical purpose, the present invention by the following technical solutions:
For an Avianreovirus suppressing method for Embryo Gallus domesticus, the method is to egg injection chicken alpha interferon.
Preferably, described Embryo Gallus domesticus is in 9 ~ 11 ages in days.
Preferably, injected dose is 20 ~ 40 μ g, more optimizedly 30 μ g.
Preferably, described Embryo Gallus domesticus is the Embryo Gallus domesticus having infected Avianreovirus.
Preferably, described Embryo Gallus domesticus is SPF Embryo Gallus domesticus.
Passing through egg injection chicken alpha interferon of novelty of the present invention, makes the resist effect of Embryo Gallus domesticus to Avianreovirus significantly strengthen, obviously inhibits the propagation of Avianreovirus.In optimal technical scheme, being preferably 9 ~ 11 ages in days is find that the inhibition of this condition hemostasis chicken alpha interferon to virus is best; The dosage being preferably 20 ~ 40 μ g can either ensure that excellent HIV suppression effect guarantees safety again; In addition, the inventive method both may be used for catching an illness the HIV suppression of Embryo Gallus domesticus, also may be used for the prevention of healthy Embryo Gallus domesticus to virus.The present invention utilizes chicken alpha interferon to suppress Avianreovirus in Embryo Gallus domesticus cleverly, fowl poultry immune ability can be strengthened on the basis avoiding drug resistance problems, the present invention is remarkably productive, simultaneously cost lower, be easy to realize, therefore there is outstanding promotion prospect.
Detailed description of the invention
Below will be described in detail the specific embodiment of the present invention.In order to avoid too much unnecessary details, in the examples below to belonging to known structure or function will not be described in detail.Apart from outside definition, technology used in following examples and scientific terminology have the identical meanings generally understood with those skilled in the art of the invention.
Embodiment 1
1, the EID of Avianreovirus
50mensuration
Avianreovirus liquid sterile saline is made 10 times of serial dilutions respectively, gets 10
-6, 10
-7, 10
-8with 10
-9inoculation 9 ~ 11 age in days SPF Embryo Gallus domesticus 5 in 4 each allantocherion of dilution factor, every embryo 0.2ml, puts 37 DEG C and continues to hatch 7, and Embryo Gallus domesticus dead before 24 hours discards to be disregarded, and the Embryo Gallus domesticus dead at 48 ~ 120 hours takes out at any time, observes and judges pathological changes, calculates ELD
50.
Avianreovirus content is 10 as calculated
8.0eLD
50/ 0.2ml.
2, chicken alpha interferon in Embryo Gallus domesticus on the impact that Avianreovirus copies
The Embryo Gallus domesticus of 10 age in days SPF is divided into 4 groups, often organizes 6 pieces.The chicken alpha interferon of the 1st group of Simultaneous vaccination 30ug and 100EID
50avianreovirus liquid; First inoculate 100EID for 2nd group
50avianreovirus liquid, inoculate 30ug chicken alpha interferon after 30 hours; Only inoculate 100EID for 3rd group
50avianreovirus liquid in contrast; 4th group of inoculation 30ug interferon.Put into incubator to hatch, record chicken embryo death situation.Result is as shown in table 1:
What table 1 chicken alpha interferon copied Avianreovirus in Embryo Gallus domesticus affects experimental result
As shown in table 1, the mean time to death of the 1st group of Embryo Gallus domesticus is at 54 hours; The mean time to death of the 2nd group is at 43 hours, and the mean time to death of the 3rd group of positive controls is at about 37h; 4th group of Embryo Gallus domesticus growth is normal.The Embryo Gallus domesticus mean time to death adding chicken alpha interferon is all late than positive controls.Along with the difference of access chicken alpha interferon time, the mean time to death of the 1st group and the 2nd group also has difference; The death time of infection Embryo Gallus domesticus can be extended after access chicken alpha interferon, and the difference of chicken alpha interferon turn-on time, also there is difference the death time.The chicken embryo death time simultaneously accessing chicken alpha interferon and virus liquid is more longer than the time-to-live of access chicken alpha interferon after first access virus.As can be seen here, the propagation of Avianreovirus significantly can be suppressed to egg injection chicken alpha interferon.
Embodiment 2
For an Avianreovirus suppressing method for Embryo Gallus domesticus, the method is to egg injection chicken alpha interferon.Wherein said Embryo Gallus domesticus is in 9 ages in days; Injected dose is 20 μ g; Described Embryo Gallus domesticus is the Embryo Gallus domesticus having infected Avianreovirus.
Embodiment 3
For an Avianreovirus suppressing method for Embryo Gallus domesticus, the method is to egg injection chicken alpha interferon.Wherein said Embryo Gallus domesticus is in 11 ages in days; Injected dose is 40 μ g; Described Embryo Gallus domesticus is SPF Embryo Gallus domesticus.
Embodiment 4
For an Avianreovirus suppressing method for Embryo Gallus domesticus, the method is to egg injection chicken alpha interferon.
Above embodiments of the invention have been described in detail, but described content is only preferred embodiment of the present invention, not in order to limit the present invention.All make in application range of the present invention any amendment, equivalent to replace and improvement etc., all should be included within protection scope of the present invention.
Claims (5)
1., for an Avianreovirus suppressing method for Embryo Gallus domesticus, it is characterized in that egg injection chicken alpha interferon.
2. method according to claim 1, is characterized in that described Embryo Gallus domesticus is in 9 ~ 11 ages in days.
3. method according to claim 1, is characterized in that injected dose is 20 ~ 40 μ g.
4. method according to claim 1, is characterized in that described Embryo Gallus domesticus is the Embryo Gallus domesticus having infected Avianreovirus.
5. method according to claim 1, is characterized in that described Embryo Gallus domesticus is SPF Embryo Gallus domesticus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510411853.9A CN105031620A (en) | 2015-07-14 | 2015-07-14 | Avian reovirus inhibition method aiming at chick embryos |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510411853.9A CN105031620A (en) | 2015-07-14 | 2015-07-14 | Avian reovirus inhibition method aiming at chick embryos |
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| CN105031620A true CN105031620A (en) | 2015-11-11 |
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| CN201510411853.9A Pending CN105031620A (en) | 2015-07-14 | 2015-07-14 | Avian reovirus inhibition method aiming at chick embryos |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113663053A (en) * | 2021-08-20 | 2021-11-19 | 广西壮族自治区兽医研究所 | Application of IFI6 protein or substance for regulating IFI6 protein gene expression in preparation of avian reovirus inhibitor |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87100480A (en) * | 1986-01-24 | 1987-11-11 | 基因技术公司 | Methods and formulations for preventing and treating viral infections |
-
2015
- 2015-07-14 CN CN201510411853.9A patent/CN105031620A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN87100480A (en) * | 1986-01-24 | 1987-11-11 | 基因技术公司 | Methods and formulations for preventing and treating viral infections |
Non-Patent Citations (3)
| Title |
|---|
| 丰宗洋: "鸡α干扰素的原核表达及其抗病毒效果研究", 《中国优秀硕士学位论文全文数据库》 * |
| 山东宝来利来生物工程股份有限公司等: "鸡α干扰素基因的人工合成、表达及抗病毒活性研究", 《中国饲料》 * |
| 葛忠源等: "鸡干扰素研究进展", 《家禽科学》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113663053A (en) * | 2021-08-20 | 2021-11-19 | 广西壮族自治区兽医研究所 | Application of IFI6 protein or substance for regulating IFI6 protein gene expression in preparation of avian reovirus inhibitor |
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