CN104982333A - Orchid seedling propagating method - Google Patents
Orchid seedling propagating method Download PDFInfo
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- CN104982333A CN104982333A CN201510360930.2A CN201510360930A CN104982333A CN 104982333 A CN104982333 A CN 104982333A CN 201510360930 A CN201510360930 A CN 201510360930A CN 104982333 A CN104982333 A CN 104982333A
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Abstract
The invention provides an orchid seedling propagating method. The method comprises the following steps that seedling tempering is carried out: under the condition of the indoor natural light, a bottle cap is opened, a green root seedling is subjected to transition for two weeks and taken out, and a medium attached to the root of the seedling is washed for preparing; mycorrhization is carried out on the seedling: the taken seedling is covered and implanted in a cell pan by the medium containing with mycorrhiza fungi, in the appropriate temperature, the seedling is cultivated in a cool and ventilated place, during the cultivation, water is sprayed on the leaf part, a new root is facilitated to be grown, four weeks later, the new root is transferred into a greenhouse to be subjected to normal water and fertilizer managing, and eight months later, a mycorrhization seedling is obtained; the medium comprises, by mass, 0.5 part of peat, 0.25 part of dry rot soil and 0.25 part of red soil, and the medium is obtained by adding oak leaf three-grade bacteria with bacterium liquid. The orchid seedling propagating method has the advantages that the orchid quality can be improved, and it is guaranteed that the orchid can grow in a healthy mode.
Description
Technical field
The present invention relates to planting technology field, particularly relate to a kind of orchid germchit propagating method.
Background technology
Orchid refers to the general name of whole orchid (Orchidaceae).In China, traditional " orchid " concept only refers to the orchid family, Cymbidium (Cymbidium) plant that originate in China, and claim state blue, ground is raw, therefore also known as Terrestrial orchid.Orchid is the high rare species of a class ornamental value and economic worth.
Orchid in Chinese tradition famous flower only refers to the several Terrestrial orchid be distributed in Cymbidium Plants, as Chunlan, Hui Lan, sword-leaved cymbidium, Chinese cymbidium and cold blue etc., i.e. and " China blue " of indication usually.The tropical orchid of this class orchid and large flower and brilliant color differs widely, and does not have eye-catching gorgeous state, does not have very large flower, leaf, but has simple gentle and quiet, simple and elegant noble and unsullied makings, meets very much the aesthetical standard of Asians.In the cultivation history in state-owned more than 1,000 years.
But the current inadequate science of orchid sapling multiplication, causes the poor quality of orchid, yields poorly.
Summary of the invention
In order to solve the problems of the prior art, the invention provides a kind of orchid germchit propagating method.
The invention provides a kind of orchid germchit propagating method, comprise the steps:
Hardening: seedling of taking root takes out after uncork lid transition 2 weeks under Indoor Natural illumination condition, clean attach at the medium of seedling root for subsequent use;
Nursery stock Mycorrhizal: the matrix parcel of the seedling of taking-up containing mycorrhizal fungi is planted in the dish of cave, under mild temperature, be placed in the cultivation of shady and cool ventilation place, period is to leaf portion spray water, promote new root, move into after 4 weeks in greenhouses and carry out normal water, fertile management, after August Mycorrhizal seedling; Described matrix obtains adding the robur leaf three-class strain being mixed with bacterium liquid in 1/2 part of peat, 1/4 part of dry detritus soil and 1/4 part of laterite a little respectively by weight.
As a further improvement on the present invention, Seed Germination comprises the following steps:
A. the selection of explant: the capsule selecting Chunlan not split is explant;
B. seed treatment: the process of powder shape seed asepsis will be taken out after Chunlan capsule aseptic process;
C. seed germination: it is protocorm that treated Chunlan seed is evenly sowed incubation growth on seed germination medium; The culture medium prescription used is: 1/2MS+NAA0.6 ~ 0.8mg/L+IBA2 ~ 2mg/L+80ml/L coconut palm breast+mannitol 1g/L+ activated carbon 0.5g/L+ sucrose 25g/L, medium PH value is 5.0 ~ 6.0.
As a further improvement on the present invention, protocorm shoot proliferation process is by the protocorm of Initial culture and bud shoot proliferation cultivation 65-70 days on medium respectively, grows up to sprouting or protocorm, constantly cuts protocorm subculture later, get final product geometric growth; The culture medium prescription used is: 1/2MS+NAA0.3 ~ 0.6mg/L+6-BA0.5 ~ 0.7mg/L+ activated carbon 0.8g/L+ sucrose 28g/L, medium PH value is 5.0 ~ 6.0.
As a further improvement on the present invention, process of rooting culture is proceeded on root media by underdeveloped for larger root system seedling to cultivate, and make rooting rate reach more than 95%, plant strain growth is vigorous, forms the seedling of 6 ~ 8cm after 6 weeks; The culture medium prescription used is: 1/2MS+NAA0.8mg/L+ activated carbon 0.8g/L+ sucrose 28g/L+ agar 5g/L, medium PH value is 5.0 ~ 6.0.
The invention has the beneficial effects as follows: adopt orchid germchit propagating method of the present invention, the quality of orchid can be promoted, ensure the growth of orchid health.
Embodiment
The invention discloses a kind of orchid germchit propagating method, its seedling obtains and mainly comprises seed germination, protocorm shoot proliferation and process of rooting culture, it is characterized in that this propagation method also carries out the take root hardening of seedling and the Mycorrhizal process with mycorrhizal fungi after seedling obtains, be specially:
Hardening: seedling of taking root takes out after uncork lid transition 2 weeks under Indoor Natural illumination condition, clean attach at the medium of seedling root for subsequent use;
Nursery stock Mycorrhizal: the matrix parcel of the seedling of taking-up containing mycorrhizal fungi is planted in the dish of cave, under mild temperature, be placed in the cultivation of shady and cool ventilation place, period is to leaf portion spray water, promote new root, move into after 4 weeks in greenhouses and carry out normal water, fertile management, after August Mycorrhizal seedling; Described matrix obtains adding the robur leaf three-class strain being mixed with bacterium liquid in 1/2 part of peat, 1/4 part of dry detritus soil and 1/4 part of laterite a little respectively by weight.
Seed Germination comprises the following steps:
A. the selection of explant: the capsule selecting Chunlan not split is explant;
B. seed treatment: the process of powder shape seed asepsis will be taken out after Chunlan capsule aseptic process;
C. seed germination: it is protocorm that treated Chunlan seed is evenly sowed incubation growth on seed germination medium; The culture medium prescription used is: 1/2MS+NAA0.6 ~ 0.8mg/L+IBA2 ~ 2mg/L+80ml/L coconut palm breast+mannitol 1g/L+ activated carbon 0.5g/L+ sucrose 25g/L, medium PH value is 5.0 ~ 6.0.
Protocorm shoot proliferation process is by the protocorm of Initial culture and bud shoot proliferation cultivation 65-70 days on medium respectively, grows up to sprouting or protocorm, constantly cuts protocorm subculture later, get final product geometric growth; The culture medium prescription used is: 1/2MS+NAA0.3 ~ 0.6mg/L+6-BA0.5 ~ 0.7mg/L+ activated carbon 0.8g/L+ sucrose 28g/L, medium PH value is 5.0 ~ 6.0.
Process of rooting culture is proceeded on root media by underdeveloped for larger root system seedling to cultivate, and make rooting rate reach more than 95%, plant strain growth is vigorous, forms the seedling of 6 ~ 8cm after 6 weeks; The culture medium prescription used is: 1/2MS+NAA0.8mg/L+ activated carbon 0.8g/L+ sucrose 28g/L+ agar 5g/L, medium PH value is 5.0 ~ 6.0.
The present invention is separated endogenetic fungus from wild Chunlan root, itself and the symbiosis of Chunlan plantlet in vitro are cultivated, filter out effective mycorrhizal fungi that also can it obviously can be promoted to grow with the effective symbiosis of Chunlan plantlet in vitro, Mycorrhizal cultivation is carried out to Chunlan plantlet in vitro, fundamentally overcomes test-tube plantlet breeding, lack mycorrhizal fungi, transplant aseptic seedling survival rate low, poor growth, blooms slow, spends little shortcoming of even not blooming.
Adopt orchid germchit propagating method of the present invention, the quality of orchid can be promoted, ensure the growth of orchid health.
Above content is in conjunction with concrete preferred embodiment further description made for the present invention, can not assert that specific embodiment of the invention is confined to these explanations.For general technical staff of the technical field of the invention, without departing from the inventive concept of the premise, some simple deduction or replace can also be made, all should be considered as belonging to protection scope of the present invention.
Claims (4)
1. an orchid germchit propagating method, is characterized in that, comprises the steps:
Hardening: seedling of taking root takes out after uncork lid transition 2 weeks under Indoor Natural illumination condition, clean attach at the medium of seedling root for subsequent use;
Nursery stock Mycorrhizal: the matrix parcel of the seedling of taking-up containing mycorrhizal fungi is planted in the dish of cave, under mild temperature, be placed in the cultivation of shady and cool ventilation place, period is to leaf portion spray water, promote new root, move into after 4 weeks in greenhouses and carry out normal water, fertile management, after August Mycorrhizal seedling; Described matrix obtains adding the robur leaf three-class strain being mixed with bacterium liquid in 1/2 part of peat, 1/4 part of dry detritus soil and 1/4 part of laterite a little respectively by weight.
2. orchid germchit propagating method according to claim 1, is characterized in that, Seed Germination comprises the following steps:
A. the selection of explant: the capsule selecting Chunlan not split is explant;
B. seed treatment: the process of powder shape seed asepsis will be taken out after Chunlan capsule aseptic process;
C. seed germination: it is protocorm that treated Chunlan seed is evenly sowed incubation growth on seed germination medium; The culture medium prescription used is: 1/2MS+NAA0.6 ~ 0.8mg/L+IBA2 ~ 2mg/L+80ml/L coconut palm breast+mannitol 1g/L+ activated carbon 0.5g/L+ sucrose 25g/L, medium PH value is 5.0 ~ 6.0.
3. orchid germchit propagating method according to claim 1, it is characterized in that, protocorm shoot proliferation process is by the protocorm of Initial culture and bud shoot proliferation cultivation 65-70 days on medium respectively, grow up to sprouting or protocorm, constantly cut protocorm subculture later, get final product geometric growth; The culture medium prescription used is: 1/2MS+NAA0.3 ~ 0.6mg/L+6-BA0.5 ~ 0.7mg/L+ activated carbon 0.8g/L+ sucrose 28g/L, medium PH value is 5.0 ~ 6.0.
4. orchid germchit propagating method according to claim 1, it is characterized in that, process of rooting culture is proceeded on root media by underdeveloped for larger root system seedling to cultivate, and makes rooting rate reach more than 95%, plant strain growth is vigorous, forms the seedling of 6 ~ 8cm after 6 weeks; The culture medium prescription used is: 1/2MS+NAA0.8mg/L+ activated carbon 0.8g/L+ sucrose 28g/L+ agar 5g/L, medium PH value is 5.0 ~ 6.0.
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| CN201510360930.2A CN104982333A (en) | 2015-06-26 | 2015-06-26 | Orchid seedling propagating method |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069778A (en) * | 2016-07-31 | 2016-11-09 | 张玉薇 | The method for tissue culture of Herba Cymbidii Goeringii |
| CN106234218A (en) * | 2016-07-31 | 2016-12-21 | 张玉薇 | Herba Cymbidii Goeringii tissue culture culture medium |
| CN107439259A (en) * | 2017-09-26 | 2017-12-08 | 上海应用技术大学 | A kind of preparation method of potted orchids special active cultivation matrix |
| CN107926430A (en) * | 2017-11-22 | 2018-04-20 | 荔浦县万家兴果蔬专业合作社 | A kind of high-yield method of shatian pomelo |
| CN109845609A (en) * | 2019-04-12 | 2019-06-07 | 江苏里下河地区农业科学研究所 | A kind of household balcony Chunlan cultural method |
-
2015
- 2015-06-26 CN CN201510360930.2A patent/CN104982333A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106069778A (en) * | 2016-07-31 | 2016-11-09 | 张玉薇 | The method for tissue culture of Herba Cymbidii Goeringii |
| CN106234218A (en) * | 2016-07-31 | 2016-12-21 | 张玉薇 | Herba Cymbidii Goeringii tissue culture culture medium |
| CN107439259A (en) * | 2017-09-26 | 2017-12-08 | 上海应用技术大学 | A kind of preparation method of potted orchids special active cultivation matrix |
| CN107926430A (en) * | 2017-11-22 | 2018-04-20 | 荔浦县万家兴果蔬专业合作社 | A kind of high-yield method of shatian pomelo |
| CN109845609A (en) * | 2019-04-12 | 2019-06-07 | 江苏里下河地区农业科学研究所 | A kind of household balcony Chunlan cultural method |
| CN109845609B (en) * | 2019-04-12 | 2021-06-18 | 江苏里下河地区农业科学研究所 | Household balcony cymbidium culture method |
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Application publication date: 20151021 |