CN104940999A - Vascular wall elastic basement membrane and preparation method thereof - Google Patents
Vascular wall elastic basement membrane and preparation method thereof Download PDFInfo
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- CN104940999A CN104940999A CN201510365281.5A CN201510365281A CN104940999A CN 104940999 A CN104940999 A CN 104940999A CN 201510365281 A CN201510365281 A CN 201510365281A CN 104940999 A CN104940999 A CN 104940999A
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- 210000002469 basement membrane Anatomy 0.000 title claims abstract description 39
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 230000002792 vascular Effects 0.000 title abstract 8
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 claims abstract description 58
- 229910000029 sodium carbonate Inorganic materials 0.000 claims abstract description 29
- HDTRYLNUVZCQOY-UHFFFAOYSA-N α-D-glucopyranosyl-α-D-glucopyranoside Natural products OC1C(O)C(O)C(CO)OC1OC1C(O)C(O)C(O)C(CO)O1 HDTRYLNUVZCQOY-UHFFFAOYSA-N 0.000 claims abstract description 15
- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 claims abstract description 15
- 229920002101 Chitin Polymers 0.000 claims abstract description 15
- 102000008186 Collagen Human genes 0.000 claims abstract description 15
- 108010035532 Collagen Proteins 0.000 claims abstract description 15
- 108010022355 Fibroins Proteins 0.000 claims abstract description 15
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 claims abstract description 15
- 108010013296 Sericins Proteins 0.000 claims abstract description 15
- HDTRYLNUVZCQOY-WSWWMNSNSA-N Trehalose Natural products O[C@@H]1[C@@H](O)[C@@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-WSWWMNSNSA-N 0.000 claims abstract description 15
- HDTRYLNUVZCQOY-LIZSDCNHSA-N alpha,alpha-trehalose Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@@H]1O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 HDTRYLNUVZCQOY-LIZSDCNHSA-N 0.000 claims abstract description 15
- 239000001913 cellulose Substances 0.000 claims abstract description 15
- 229920002678 cellulose Polymers 0.000 claims abstract description 15
- 239000008367 deionised water Substances 0.000 claims abstract description 15
- 229910021641 deionized water Inorganic materials 0.000 claims abstract description 15
- 229920002674 hyaluronan Polymers 0.000 claims abstract description 15
- 229960003160 hyaluronic acid Drugs 0.000 claims abstract description 15
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 15
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 claims abstract description 12
- 239000004472 Lysine Substances 0.000 claims abstract description 12
- 239000002994 raw material Substances 0.000 claims abstract description 8
- 229920001436 collagen Polymers 0.000 claims abstract description 4
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 claims description 14
- 239000012530 fluid Substances 0.000 claims description 14
- 239000013521 mastic Substances 0.000 claims description 14
- 239000000843 powder Substances 0.000 claims description 14
- 238000001035 drying Methods 0.000 claims description 7
- 239000000203 mixture Substances 0.000 claims description 7
- 239000002245 particle Substances 0.000 claims description 7
- 238000010298 pulverizing process Methods 0.000 claims description 7
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 claims description 6
- 229910021529 ammonia Inorganic materials 0.000 claims description 3
- 108020001775 protein parts Proteins 0.000 claims description 3
- 230000000694 effects Effects 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 231100000252 nontoxic Toxicity 0.000 abstract description 2
- 230000003000 nontoxic effect Effects 0.000 abstract description 2
- 239000000463 material Substances 0.000 description 7
- 210000001519 tissue Anatomy 0.000 description 6
- 239000012620 biological material Substances 0.000 description 5
- 210000000056 organ Anatomy 0.000 description 4
- 210000004204 blood vessel Anatomy 0.000 description 3
- 230000010261 cell growth Effects 0.000 description 3
- 208000032594 Vascular Remodeling Diseases 0.000 description 2
- 239000003519 biomedical and dental material Substances 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 206010061218 Inflammation Diseases 0.000 description 1
- 230000000680 avirulence Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000033558 biomineral tissue development Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 230000008595 infiltration Effects 0.000 description 1
- 238000001764 infiltration Methods 0.000 description 1
- 230000004054 inflammatory process Effects 0.000 description 1
- 210000004379 membrane Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 231100000957 no side effect Toxicity 0.000 description 1
- 230000008467 tissue growth Effects 0.000 description 1
Landscapes
- Materials For Medical Uses (AREA)
Abstract
The invention discloses a vascular wall elastic basement membrane and a preparation method thereof. The vascular wall elastic basement membrane is prepared from the following raw materials in parts by mass: 12-28 parts of collagens, 13-26 parts of hyaluronic acid, 12-30 parts of chitin, 10-22 parts of trehalose, 12-30 parts of fibroin, 12-20 parts of sericin, 8-22 parts of proline, 9-24 parts of lysine, 12-35 parts of cellulose, 1-7 parts of sodium carbonate and 25-60 parts of deionized water. Compared with the prior art, the vascular wall elastic basement membrane disclosed by the invention has the following advantages that (1) the prepared vascular wall elastic basement membrane is capable of effectively remodelling a vascular wall and increasing the success rate of a vascular remodelling operation; and (2) the prepared vascular wall elastic basement membrane is safe and non-toxic, free from side effects, convenient to use, and low in production cost.
Description
Technical field
The present invention relates to biological engineering field of new, particularly relate to a kind of vessel wall elasticity basement membrane and preparation method thereof.
Background technology
Along with the development of society, the progress of science, result in interdisciplinary mutual embedding branch and infiltration.The bio-medical material interpenetrated by the multiple subject such as modern life science, material science, medical science, engineering and develop and produce along with people to healthy, quality of life standard raising and more receive the concern of people, become the focus that countries in the world are are competitively researched and developed.Bio-medical material, also known as biomaterial, refers to for the purpose of medical treatment, be used for diagnosing, treat, repair or replace its disease damage tissue, organ to organism or promote its function without the novel High-tech Material of life.Biomaterial comprises tissue engineering material, nano meter biomaterial, biomineralization material and composite biological material etc.
Wherein organizational project refer to that application life sciences and the principle of engineering and method design, build a biological device to safeguard human body cell and tissue growth, recover the tissue of damaged or the function of organ.Over nearly 10 years, tissue engineering develops into the cross discipline integrating biological engineering, cytobiology, molecular biology, biomaterial, biotechnology, biochemistry, biomechanics and clinical medicine.
Tissue engineering material is the biodegradable polymeric material in a class implantable.In the incubation of tissue, organ, play temporary support effect, meanwhile, its geometry can guide the shape of the organ needed for Growth of Cells one-tenth, and therefore, these host materials must have following performance: the space that can provide Growth of Cells and adhesion; Biodegradable; There is good organism adaptability; Avirulence.
Basement membrane is the important component part of blood vessel wall, and in vascular remodeling operation, the structure of basement membrane is a significant process, but the structure blood vessel wall basement membrane that the means also do not had in prior art can be effective, safe.
Summary of the invention
The technical problem that the present invention solves: in order to obtain a kind of effectively, safely, be applicable to the basement membrane that human vas wall reinvents, the invention provides a kind of vessel wall elasticity basement membrane and preparation method thereof.
To achieve these goals, the present invention is by the following technical solutions:
A kind of vessel wall elasticity basement membrane, makes by mass fraction proportioning primarily of following raw material: collagen protein 12 ~ 28 parts, hyaluronic acid 13 ~ 26 parts, chitin 12 ~ 30 parts, trehalose 10 ~ 22 parts, fibroin 12 ~ 30 parts, sericin 12 ~ 20 parts, proline 8 ~ 22 parts, lysine 9 ~ 24 parts, cellulose 12 ~ 35 parts, sodium carbonate 1 ~ 7 part, deionized water 25 ~ 60 parts.
As a preferred embodiment of the present invention, described vessel wall elasticity basement membrane is made by mass fraction proportioning primarily of following raw material: collagen xxii protein part, hyaluronic acid 21 parts, chitin 25 parts, trehalose 18 parts, fibroin 19 parts, sericin 14 parts, proline 12 parts, bad ammonia 17 parts, cellulose 32 parts, sodium carbonate 5 parts, deionized water 50 parts.
A preparation method for vessel wall elasticity basement membrane, comprises following steps:
(1) sodium carbonate is dissolved in deionized water, preparation mass concentration is the sodium carbonate liquor of 0.3 ~ 1.9%, and fibroin, sericin, proline and lysine 1 ~ 3:4 in mass ratio ~ 7:0.1 ~ 0.5:0.3 ~ 0.7 is dissolved in sodium carbonate liquor is uniformly mixed, obtained base fluid;
(2) chitin, cellulose and trehalose are placed in reactor, temperature be 630 ~ 820 DEG C, pressure makes fused mass under being the condition of 2.2 ~ 4.1MPa, and powder is made in drying, pulverizing, and particle diameter is 1000 ~ 1500 orders;
(3) mixture of powders that collagen protein, hyaluronic acid and step (2) obtain is added in the base fluid of step (1), temperature be 630 ~ 820 DEG C, rotating speed is stirred in thick mastic under being 800 ~ 1600 revs/min of conditions;
(4) mastic that step (3) obtains is coated in mould, 650 ~ 820 DEG C, compressing under 2.7 ~ 4.1MPa condition, obtained vessel wall elasticity basement membrane.
As a preferred technical solution of the present invention, sodium carbonate is dissolved in deionized water in (1) by step, preparation mass concentration is the sodium carbonate liquor of 1.2%, and by fibroin, sericin, proline and lysine in mass ratio 2:5:0.3:0.5 be dissolved in sodium carbonate liquor and be uniformly mixed, obtained base fluid.
As a preferred technical solution of the present invention, chitin, cellulose and trehalose are placed in reactor in (2) by step, temperature be 755 DEG C, pressure makes fused mass under being the condition of 3.6MPa, and powder is made in drying, pulverizing, and particle diameter is 1400 orders.
As a preferred technical solution of the present invention, in step (3), the mixture of powders that collagen protein, hyaluronic acid and step (2) obtain is added in the base fluid of step (1), temperature be 755 DEG C, rotating speed is stirred in thick mastic under being 1400 revs/min of conditions.
As a preferred technical solution of the present invention, in step (4), the mastic that step (3) obtains is coated in mould, 755 DEG C, compressing under 3.6MPa condition, obtained vessel wall elasticity basement membrane.
beneficial effect
Compared with prior art, the present invention has the following advantages:
(1) the vessel wall elasticity basement membrane that the present invention obtains effectively can reinvent blood vessel wall, improves vascular remodeling success rate of operation;
(2) the vessel wall elasticity basement membrane that obtains of the present invention can safety non-toxic, have no side effect, easy to use, and production cost is low.
Detailed description of the invention
Embodiment 1:
A kind of vessel wall elasticity basement membrane, makes by mass fraction proportioning primarily of following raw material: collagen protein 12 parts, hyaluronic acid 13 parts, chitin 12 parts, trehalose 10 parts, fibroin 12 parts, sericin 12 parts, proline 8 parts, lysine 9 parts, cellulose 12 parts, sodium carbonate 1 part, deionized water 25 parts.
A preparation method for vessel wall elasticity basement membrane, comprises following steps:
(1) be dissolved in deionized water by sodium carbonate, preparation mass concentration is the sodium carbonate liquor of 0.3%, and by fibroin, sericin, proline and lysine in mass ratio 1:4:0.1:0.3 be dissolved in sodium carbonate liquor and be uniformly mixed, obtained base fluid;
(2) chitin, cellulose and trehalose are placed in reactor, temperature be 630 DEG C, pressure makes fused mass under being the condition of 2.2MPa, and powder is made in drying, pulverizing, and particle diameter is 1000 orders;
(3) mixture of powders that collagen protein, hyaluronic acid and step (2) obtain is added in the base fluid of step (1), temperature be 630 DEG C, rotating speed is stirred in thick mastic under being 800 revs/min of conditions;
(4) mastic that step (3) obtains is coated in mould, 650 DEG C, compressing under 2.7MPa condition, obtained vessel wall elasticity basement membrane.
Embodiment 2:
A kind of vessel wall elasticity basement membrane, makes by mass fraction proportioning primarily of following raw material: collagen xxii protein part, hyaluronic acid 21 parts, chitin 25 parts, trehalose 18 parts, fibroin 19 parts, sericin 14 parts, proline 12 parts, bad ammonia 17 parts, cellulose 32 parts, sodium carbonate 5 parts, deionized water 50 parts.
A preparation method for vessel wall elasticity basement membrane, comprises following steps:
(1) be dissolved in deionized water by sodium carbonate, preparation mass concentration is the sodium carbonate liquor of 1.2%, and by fibroin, sericin, proline and lysine in mass ratio 2:5:0.3:0.5 be dissolved in sodium carbonate liquor and be uniformly mixed, obtained base fluid;
(2) chitin, cellulose and trehalose are placed in reactor, temperature be 755 DEG C, pressure makes fused mass under being the condition of 3.6MPa, and powder is made in drying, pulverizing, and particle diameter is 1400 orders;
(3) mixture of powders that collagen protein, hyaluronic acid and step (2) obtain is added in the base fluid of step (1), temperature be 755 DEG C, rotating speed is stirred in thick mastic under being 1400 revs/min of conditions;
(4) mastic that step (3) obtains is coated in mould, 755 DEG C, compressing under 3.6MPa condition, obtained vessel wall elasticity basement membrane.
Embodiment 3:
A kind of vessel wall elasticity basement membrane, makes by mass fraction proportioning primarily of following raw material: collagen protein 28 parts, hyaluronic acid 26 parts, chitin 30 parts, trehalose 22 parts, fibroin 30 parts, sericin 20 parts, proline 22 parts, lysine 24 parts, cellulose 35 parts, sodium carbonate 7 parts, deionized water 60 parts.
A preparation method for vessel wall elasticity basement membrane, comprises following steps:
(1) be dissolved in deionized water by sodium carbonate, preparation mass concentration is the sodium carbonate liquor of 1.9%, and by fibroin, sericin, proline and lysine in mass ratio 3:7:0.5:0.7 be dissolved in sodium carbonate liquor and be uniformly mixed, obtained base fluid;
(2) chitin, cellulose and trehalose are placed in reactor, temperature be 820 DEG C, pressure makes fused mass under being the condition of 4.1MPa, and powder is made in drying, pulverizing, and particle diameter is 1500 orders;
(3) mixture of powders that collagen protein, hyaluronic acid and step (2) obtain is added in the base fluid of step (1), temperature be 820 DEG C, rotating speed is stirred in thick mastic under being 1600 revs/min of conditions;
(4) mastic that step (3) obtains is coated in mould, 820 DEG C, compressing under 4.1MPa condition, obtained vessel wall elasticity basement membrane.
The vessel wall elasticity basement membrane obtained to embodiment 1 ~ 3 detects, and result is as follows:
| Membrane aperture | The new capillary vessel used time | Inflammatory reaction | |
| Embodiment 1 | 110 microns | 7 days | Nothing |
| Embodiment 2 | 85 microns | 4 days | Nothing |
| Embodiment 3 | 90 microns | 6 days | Nothing |
Claims (7)
1. a vessel wall elasticity basement membrane, it is characterized in that, make by mass fraction proportioning primarily of following raw material: collagen protein 12 ~ 28 parts, hyaluronic acid 13 ~ 26 parts, chitin 12 ~ 30 parts, trehalose 10 ~ 22 parts, fibroin 12 ~ 30 parts, sericin 12 ~ 20 parts, proline 8 ~ 22 parts, lysine 9 ~ 24 parts, cellulose 12 ~ 35 parts, sodium carbonate 1 ~ 7 part, deionized water 25 ~ 60 parts.
2. a kind of vessel wall elasticity basement membrane according to claim 1, it is characterized in that, make by mass fraction proportioning primarily of following raw material: collagen xxii protein part, hyaluronic acid 21 parts, chitin 25 parts, trehalose 18 parts, fibroin 19 parts, sericin 14 parts, proline 12 parts, bad ammonia 17 parts, cellulose 32 parts, sodium carbonate 5 parts, deionized water 50 parts.
3. the preparation method of a kind of vessel wall elasticity basement membrane according to claim 1, is characterized in that, comprise following steps:
(1) sodium carbonate is dissolved in deionized water, preparation mass concentration is the sodium carbonate liquor of 0.3 ~ 1.9%, and fibroin, sericin, proline and lysine 1 ~ 3:4 in mass ratio ~ 7:0.1 ~ 0.5:0.3 ~ 0.7 is dissolved in sodium carbonate liquor is uniformly mixed, obtained base fluid;
(2) chitin, cellulose and trehalose are placed in reactor, temperature be 630 ~ 820 DEG C, pressure makes fused mass under being the condition of 2.2 ~ 4.1MPa, and powder is made in drying, pulverizing, and particle diameter is 1000 ~ 1500 orders;
(3) mixture of powders that collagen protein, hyaluronic acid and step (2) obtain is added in the base fluid of step (1), temperature be 630 ~ 820 DEG C, rotating speed is stirred in thick mastic under being 800 ~ 1600 revs/min of conditions;
(4) mastic that step (3) obtains is coated in mould, 650 ~ 820 DEG C, compressing under 2.7 ~ 4.1MPa condition, obtained vessel wall elasticity basement membrane.
4. the preparation method of a kind of vessel wall elasticity basement membrane according to claim 3, it is characterized in that, sodium carbonate is dissolved in deionized water in (1) by step, preparation mass concentration is the sodium carbonate liquor of 1.2%, and by fibroin, sericin, proline and lysine in mass ratio 2:5:0.3:0.5 be dissolved in sodium carbonate liquor and be uniformly mixed, obtained base fluid.
5. the preparation method of a kind of vessel wall elasticity basement membrane according to claim 3, it is characterized in that, chitin, cellulose and trehalose are placed in reactor in (2) by step, temperature be 755 DEG C, pressure makes fused mass under being the condition of 3.6MPa, and powder is made in drying, pulverizing, particle diameter is 1400 orders.
6. the preparation method of a kind of vessel wall elasticity basement membrane according to claim 3, it is characterized in that, in step (3), the mixture of powders that collagen protein, hyaluronic acid and step (2) obtain is added in the base fluid of step (1), temperature be 755 DEG C, rotating speed is stirred in thick mastic under being 1400 revs/min of conditions.
7. the preparation method of a kind of vessel wall elasticity basement membrane according to claim 3, is characterized in that, coat in mould in step (4) by the mastic that step (3) obtains, 755 DEG C, compressing under 3.6MPa condition, obtained vessel wall elasticity basement membrane.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510365281.5A CN104940999A (en) | 2015-06-29 | 2015-06-29 | Vascular wall elastic basement membrane and preparation method thereof |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201510365281.5A CN104940999A (en) | 2015-06-29 | 2015-06-29 | Vascular wall elastic basement membrane and preparation method thereof |
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| CN104940999A true CN104940999A (en) | 2015-09-30 |
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Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1461220A (en) * | 2000-08-21 | 2003-12-10 | 伯纳德·奥布赖恩显微外科研究院 | Vascularised tissue graft |
| CN1919354A (en) * | 2006-08-30 | 2007-02-28 | 郑军 | Artificial blood vessel silk fibroin and collagen blending pre-coagulation coating |
| CN101703796A (en) * | 2009-11-27 | 2010-05-12 | 天津大学 | Nano fibre artificial vascular graft modifying internal layer and preparation method thereof |
| CN101934091A (en) * | 2010-09-07 | 2011-01-05 | 中国海洋大学 | A polysaccharide artificial blood vessel and its preparation method and application |
| CN102784015A (en) * | 2012-08-30 | 2012-11-21 | 广州迈普再生医学科技有限公司 | Artificial blood vessel loaded with pseudo-ginseng medicines, and preparation method and application for artificial blood vessel |
| CN103800097A (en) * | 2012-11-14 | 2014-05-21 | 深圳迈普再生医学科技有限公司 | Fibrous membrane for tissue repair and manufacturing method and application thereof |
| CN104174065A (en) * | 2014-09-02 | 2014-12-03 | 青岛博益特生物材料有限公司 | Absorbable artificial blood vessel as well as preparation method and application thereof |
-
2015
- 2015-06-29 CN CN201510365281.5A patent/CN104940999A/en active Pending
Patent Citations (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1461220A (en) * | 2000-08-21 | 2003-12-10 | 伯纳德·奥布赖恩显微外科研究院 | Vascularised tissue graft |
| CN1919354A (en) * | 2006-08-30 | 2007-02-28 | 郑军 | Artificial blood vessel silk fibroin and collagen blending pre-coagulation coating |
| CN101703796A (en) * | 2009-11-27 | 2010-05-12 | 天津大学 | Nano fibre artificial vascular graft modifying internal layer and preparation method thereof |
| CN101934091A (en) * | 2010-09-07 | 2011-01-05 | 中国海洋大学 | A polysaccharide artificial blood vessel and its preparation method and application |
| CN102784015A (en) * | 2012-08-30 | 2012-11-21 | 广州迈普再生医学科技有限公司 | Artificial blood vessel loaded with pseudo-ginseng medicines, and preparation method and application for artificial blood vessel |
| CN103800097A (en) * | 2012-11-14 | 2014-05-21 | 深圳迈普再生医学科技有限公司 | Fibrous membrane for tissue repair and manufacturing method and application thereof |
| CN104174065A (en) * | 2014-09-02 | 2014-12-03 | 青岛博益特生物材料有限公司 | Absorbable artificial blood vessel as well as preparation method and application thereof |
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Application publication date: 20150930 |