CN104931316A - Cell preserving and centrifugal filtering device - Google Patents
Cell preserving and centrifugal filtering device Download PDFInfo
- Publication number
- CN104931316A CN104931316A CN201510311845.7A CN201510311845A CN104931316A CN 104931316 A CN104931316 A CN 104931316A CN 201510311845 A CN201510311845 A CN 201510311845A CN 104931316 A CN104931316 A CN 104931316A
- Authority
- CN
- China
- Prior art keywords
- hollow tubular
- conduit
- transparent column
- preserves
- column hollow
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000001914 filtration Methods 0.000 title abstract description 7
- 238000012864 cross contamination Methods 0.000 abstract description 2
- 239000011521 glass Substances 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 25
- 239000007788 liquid Substances 0.000 description 12
- 238000005070 sampling Methods 0.000 description 8
- 238000001514 detection method Methods 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 238000005516 engineering process Methods 0.000 description 3
- 238000004804 winding Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 238000004062 sedimentation Methods 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 210000000270 basal cell Anatomy 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 238000007654 immersion Methods 0.000 description 1
- 210000004969 inflammatory cell Anatomy 0.000 description 1
- 239000012528 membrane Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Landscapes
- Centrifugal Separators (AREA)
Abstract
The invention discloses a cell preserving and centrifugal filtering device. The cell preserving and centrifugal filtering device comprises a transparent cylindrical hollow tube, wherein a funneled filter funnel is arranged on the upper inner side of the transparent cylindrical hollow tube; a guide pipe is arranged at the bottom of the filter funnel; a filter screen is arranged between the guide pipe and the filter funnel; the bottom of the transparent cylindrical hollow tube is in threaded connection with a bottom cover; a support pillar is arranged on the upper surface of the bottom cover; a circular-arc-shaped groove is formed in one side of the transparent cylindrical hollow tube; a glass slide is inserted into the transparent cylindrical hollow tube through the circular-arc-shaped groove. The cell preserving and centrifugal filtering device has the advantages that the structure is simple, the functions are diversified, the working efficiency is effectively improved, consumables are reduced, disorder is prevented, and the probability of cross contamination is reduced.
Description
Technical field
The present invention relates to cell detection apparatus field, particularly relate to a kind of cell and preserve centrifugal filter device.
Background technology
Learn a skill in film-making process at liquid basal cell, when centrifugal filtration and sedimentation film-making are carried out to cell, need to use sample transfer, pelleter, move liquid syringe, multiple instrument and equipment and the device such as centrifuge tube, preparation bin (or film-making folder), microslide, cost is higher, in operating process, sample needs manually transfer or application of sample between multiple instrument and equipment, and process is loaded down with trivial details, and operation is tediously long; And many parts of cell samples are shifted and application of sample time, easily occur manual operation puts upside down entanglement cross-contamination phenomena, have a strong impact on the accuracy of cell detection; Sample is easily lost and pollutes people, thing and environment in transfer process, causes the rate of missed diagnosis of cell detection to increase, and bio-safety harm hidden danger increases.
Summary of the invention
The object of this invention is to provide a kind of cell and preserve centrifugal filter device, diverse in function, effectively increases work efficiency, and reduces consumptive material, cost-saving, simplifies operation, prevents cross pollution.
In order to solve the problem existing for background technology, the present invention by the following technical solutions:
Cell preserves centrifugal filter device, include transparent column hollow tubular, the upper inner of described transparent column hollow tubular is provided with funnelform pan excessively, the described bottom crossing pan is provided with conduit, filter screen is provided with between described conduit and mistake pan, the bottom thread of described transparent column hollow tubular is connected with bottom, the upper surface of described bottom is provided with support column, the side of described transparent column hollow tubular offers arc groove, microslide is inserted in transparent column hollow tubular pipe by arc groove, and described transparent column hollow tubular is provided with top cover.
Outside described transparent column hollow tubular, upper 1/3 both sides, place are respectively provided with a hanging column, are convenient to hang on the rotating disk of hydro-extractor.
Described top cover spiral is connected on transparent column hollow tubular, and after clinician completes and draws materials, top cover of outwarding winding, puts into sampling brush, then screw, thus makes the present invention have cell preservation effect.
The bottom inside of described transparent column hollow tubular is provided with internal thread, described bottom is provided with external thread, bottom is threaded with transparent column hollow tubular, bottom is by threaded adjusting, support column on adjustment bottom and the distance between conduit, thus microslide can be supported by support column, make microslide can be close to the bottom of conduit, when microslide being taken off, only bottom need be unscrewed, microslide is separated with conduit, microslide is taken out from arc groove, just can detect the cell of sedimentation on microslide.
The lower edge of described conduit is coated with rubber cushion, makes microslide and catheter contact tightly, Leakage prevention.
The diameter of described support column, than conduit diameter is slightly large and surface is covered with skim rubber cushion, when not inserting microslide, screwing bottom, making support column just block the end opening of conduit, spill to prevent pipe inner cell sample liquid.
Bottom the upper groove surface horizontal direction of described arc groove and conduit at grade, convenient-loading slide is fixing.
The bottom surface of described bottom is arc shape, making device when placing, being merely able to put upside down, the cell specimens preserving liquid be contained in hollow tubular so just can be made to be back in pan, submergence was placed on the sampling brush in pan, ensured the sample energy liquid to be treated useful effect on sampling brush.
Beneficial effect of the present invention: structure of the present invention is simple, and diverse in function, effectively increases work efficiency, reduce consumptive material, cost-saving, simplify operation, prevent from detecting and detect rear sample liquid leaking the bio-safety harm caused, avoid cross pollution between sample, improve detection accuracy.
Accompanying drawing explanation
Fig. 1 is principle of the invention structural representation;
Fig. 2 is planar structure schematic diagram of the present invention;
Fig. 3 is planar structure schematic diagram of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the embodiment of the present invention is described:
Cell preserves centrifugal filter device, include transparent column hollow tubular 1, the upper inner of described transparent column hollow tubular 1 is provided with funnelform pan 2 excessively, the described bottom crossing pan 2 is provided with conduit 3, filter screen 4 is provided with between described conduit 3 and mistake pan 2, the bottom thread of described transparent column hollow tubular 1 is connected with bottom 5, the upper surface of described bottom 5 is provided with support column 6, the side of described transparent column hollow tubular 1 offers arc groove 7, microslide 8 inserts in transparent column hollow tubular 1 manages by arc groove 7, described transparent column hollow tubular 1 is provided with top cover 13.
The bottom inside of described transparent column hollow tubular 1 is provided with internal thread 9, described bottom 5 is provided with external thread 10, bottom 5 is threaded with transparent column hollow tubular 1, bottom 5 is by threaded adjusting, support column 6 on adjustment bottom 5 and the distance between conduit 3, thus microslide 8 can be supported by support column 6, make microslide 8 can be close to the bottom of conduit 3, when microslide 8 being taken off, only need knob bottom 5, microslide 8 is separated with conduit 3, microslide 8 is taken out from arc groove 7, just can detect the cell of deposition on microslide 8.
Outside described transparent column hollow tubular 1, upper 1/3 both sides, place are respectively provided with a hanging column 12, are convenient to hang on the rotating disk of hydro-extractor.
Described top cover 13 spiral is connected on transparent column hollow tubular 1, and after clinician completes and draws materials, top cover 13 of outwarding winding, puts into sampling brush, then screw, thus makes the present invention have cell preservation effect.
Bottom the upper groove surface horizontal direction of described arc groove 7 and conduit 3 at grade, convenient-loading slide 8 is fixing.
The lower edge of described conduit 3 is coated with rubber cushion 11, microslide 8 is contacted tightly, Leakage prevention with conduit 3.
The diameter of described support column 6, than conduit 3 diameter is slightly large and surface is covered with skim rubber cushion 11, when not inserting microslide 8, screwing bottom 5, making support column 6 just block the end opening of conduit 3, spill to prevent pipe inner cell sample liquid.
The bottom surface of described bottom 5 is arc shape, making device when placing, being merely able to put upside down, the cell treating fluid be contained in conduit 3 so just can be made to be back in pan 2, submergence was placed on the sampling brush in pan 2, ensured the sample energy liquid to be treated useful effect on sampling brush.
The present invention in use, the sampling brush speckling with cell sample was inserted in pan 2, be inverted this device, the cell specimens preserving immersion of prepackage in bottle is made not have sampling brush head a period of time, to outward winding bottom 5, after adding density separation liquid in conductive pipe 3, to insert in microslide 8 to arc groove 7 and to tighten bottom 5, carry out centrifugal on the rotating disk this device being hung over hydro-extractor, after sample screens with density centrifugation after filtration, fragment of tissue is filtered by filter screen 4, cell enters into the density separation liquid in conduit 3 through filter screen 4, under the acting in conjunction of centrifugal force and buoyancy, cell is through screening, the sick cell that density is large is deposited on microslide 8 and the microslide 8 being coated with cationic membrane firmly attracts, and the little mucilaginous substance of density and inflammatory cell are still suspended in sample rate liquid mostly.When after centrifugal end, inverted device, unscrews bottom 5, and take out microslide 8 and detect cell on microslide 8, then screwing bottom 5, the leak of liquid contaminated environment in anti-locking apparatus, causes bio-safety to endanger.This device integrates filtration, centrifugal, cell settlement, diverse in function, avoid the process that sample shifts back and forth, decrease operation steps, save detection time, effectively increase work efficiency, and reduce consumptive material, cost-saving, preventing pollution environment and bio-safety harm, avoid cross pollution between sample.
With above-mentioned foundation embodiments of the invention for enlightenment, by above-mentioned description, relevant staff in the scope not departing from the technology of the present invention thought, can carry out various change and amendment safely.Technical scope of the present invention is not limited to the content on instructions, must determine its technical scope according to right.
Claims (7)
1. cell preserves centrifugal filter device, it is characterized in that: include transparent column hollow tubular, the upper inner of described transparent column hollow tubular is provided with funnelform pan excessively, the described bottom crossing pan is provided with conduit, filter screen is provided with between described conduit and mistake pan, the bottom thread of described transparent column hollow tubular is connected with bottom, the upper surface of described bottom is provided with support column, the side of described transparent column hollow tubular offers arc groove, microslide inserts in transparent column hollow tubular pipe by arc groove, described transparent column hollow tubular is provided with top cover.
2. cell according to claim 1 preserves centrifugal filter device, it is characterized in that: outside described transparent column hollow tubular, upper 1/3 both sides, place are respectively provided with a hanging column.
3. cell according to claim 1 preserves centrifugal filter device, it is characterized in that: the bottom inside of described transparent column hollow tubular is provided with internal thread, and described bottom is provided with external thread, and bottom is threaded with transparent column hollow tubular.
4. cell according to claim 1 preserves centrifugal filter device, it is characterized in that: the lower edge of described conduit is coated with rubber cushion.
5. cell according to claim 1 preserves centrifugal filter device, it is characterized in that: the diameter of described support column is than conduit diameter is slightly large and surface is covered with skim rubber cushion.
6. cell according to claim 1 preserves centrifugal filter device, it is characterized in that: bottom the upper groove surface horizontal direction of described arc groove and conduit at grade.
7. cell according to claim 1 preserves centrifugal filter device, it is characterized in that: the bottom surface of described bottom is arc shape.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510311845.7A CN104931316B (en) | 2015-06-08 | 2015-06-08 | Cell preserves centrifugal filter device |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510311845.7A CN104931316B (en) | 2015-06-08 | 2015-06-08 | Cell preserves centrifugal filter device |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104931316A true CN104931316A (en) | 2015-09-23 |
| CN104931316B CN104931316B (en) | 2018-05-04 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201510311845.7A Expired - Fee Related CN104931316B (en) | 2015-06-08 | 2015-06-08 | Cell preserves centrifugal filter device |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN104931316B (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106680061A (en) * | 2016-12-23 | 2017-05-17 | 中山大学 | Apparatus and method for detecting parasite eggs |
| CN107807028A (en) * | 2017-11-22 | 2018-03-16 | 张祝妹 | Thinprep pap test film-making device |
| CN111051847A (en) * | 2017-07-20 | 2020-04-21 | 贝克顿·迪金森公司 | Manual method of depositing samples directly onto slides for liquid-based cytology |
| WO2020108354A1 (en) * | 2018-11-27 | 2020-06-04 | 胡学静 | Liquid-based thin-layer cell slide preparation clamp |
| CN116439232A (en) * | 2023-06-14 | 2023-07-18 | 山东第一医科大学附属省立医院(山东省立医院) | Chest surgery pathological tissue collecting and preserving device |
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| CN102539211A (en) * | 2012-01-09 | 2012-07-04 | 广州三瑞医疗器械有限公司 | Device and method for making liquid-based cytological smear in one step |
| CN202471474U (en) * | 2011-12-02 | 2012-10-03 | 孝感奥华医疗科技有限公司 | Slide making clamp for cell slide maker |
| CN203396630U (en) * | 2013-08-30 | 2014-01-15 | 杨晓斌 | Medical tablet preparing device |
| CN103616267A (en) * | 2013-11-13 | 2014-03-05 | 厦门宝太生物科技有限公司 | Integrated type cell sedimentation and smearing device and method for settling cell and preparing cell smear |
| CN203606191U (en) * | 2013-11-26 | 2014-05-21 | 上海宇度医学科技股份有限公司 | Thin-layer liquid-based cell slide maker |
-
2015
- 2015-06-08 CN CN201510311845.7A patent/CN104931316B/en not_active Expired - Fee Related
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN202471474U (en) * | 2011-12-02 | 2012-10-03 | 孝感奥华医疗科技有限公司 | Slide making clamp for cell slide maker |
| CN102539211A (en) * | 2012-01-09 | 2012-07-04 | 广州三瑞医疗器械有限公司 | Device and method for making liquid-based cytological smear in one step |
| CN203396630U (en) * | 2013-08-30 | 2014-01-15 | 杨晓斌 | Medical tablet preparing device |
| CN103616267A (en) * | 2013-11-13 | 2014-03-05 | 厦门宝太生物科技有限公司 | Integrated type cell sedimentation and smearing device and method for settling cell and preparing cell smear |
| CN203606191U (en) * | 2013-11-26 | 2014-05-21 | 上海宇度医学科技股份有限公司 | Thin-layer liquid-based cell slide maker |
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106680061A (en) * | 2016-12-23 | 2017-05-17 | 中山大学 | Apparatus and method for detecting parasite eggs |
| CN111051847A (en) * | 2017-07-20 | 2020-04-21 | 贝克顿·迪金森公司 | Manual method of depositing samples directly onto slides for liquid-based cytology |
| CN107807028A (en) * | 2017-11-22 | 2018-03-16 | 张祝妹 | Thinprep pap test film-making device |
| CN107807028B (en) * | 2017-11-22 | 2024-06-07 | 广东康乃欣生物医疗科技有限公司 | Liquid-based thin-layer cell pelleter |
| WO2020108354A1 (en) * | 2018-11-27 | 2020-06-04 | 胡学静 | Liquid-based thin-layer cell slide preparation clamp |
| CN116439232A (en) * | 2023-06-14 | 2023-07-18 | 山东第一医科大学附属省立医院(山东省立医院) | Chest surgery pathological tissue collecting and preserving device |
| CN116439232B (en) * | 2023-06-14 | 2023-09-29 | 山东第一医科大学附属省立医院(山东省立医院) | Chest surgery pathological tissue collecting and preserving device |
Also Published As
| Publication number | Publication date |
|---|---|
| CN104931316B (en) | 2018-05-04 |
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| C06 | Publication | ||
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| GR01 | Patent grant | ||
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200103 Address after: Jiangning District of Nanjing City, Jiangsu province 210000 streets moling Road No. 12 mo Zhou Patentee after: JIANGSU ZHIJU INTELLECTUAL PROPERTY SERVICE CO.,LTD. Address before: Lu'an City, Anhui province 237000 Yuan District of Pingqiao Industrial Park Patentee before: ANHUI HONGRUI BIOTECHNOLOGY Co.,Ltd. |
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| TR01 | Transfer of patent right | ||
| TR01 | Transfer of patent right |
Effective date of registration: 20200722 Address after: Room 214, 2 / F, building 1, 1111, Zhongshan North 1st Road, Hongkou District, Shanghai 200080 Patentee after: Hongzhijun Biotechnology (Shanghai) Co.,Ltd. Address before: Jiangning District of Nanjing City, Jiangsu province 210000 streets moling Road No. 12 mo Zhou Patentee before: JIANGSU ZHIJU INTELLECTUAL PROPERTY SERVICE Co.,Ltd. |
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| CF01 | Termination of patent right due to non-payment of annual fee | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20180504 |