CN104872068A - Indoor insectary and culture method for thrips hawaiiensis - Google Patents
Indoor insectary and culture method for thrips hawaiiensis Download PDFInfo
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- CN104872068A CN104872068A CN201510273796.2A CN201510273796A CN104872068A CN 104872068 A CN104872068 A CN 104872068A CN 201510273796 A CN201510273796 A CN 201510273796A CN 104872068 A CN104872068 A CN 104872068A
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- 241000365765 Thrips hawaiiensis Species 0.000 title abstract 9
- 238000012136 culture method Methods 0.000 title abstract 3
- 238000000034 method Methods 0.000 claims abstract description 51
- 230000012447 hatching Effects 0.000 claims abstract description 20
- 235000013305 food Nutrition 0.000 claims abstract description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 17
- 230000003020 moisturizing effect Effects 0.000 claims abstract description 13
- 241001414989 Thysanoptera Species 0.000 claims description 101
- 102000002322 Egg Proteins Human genes 0.000 claims description 32
- 108010000912 Egg Proteins Proteins 0.000 claims description 32
- 210000004681 ovum Anatomy 0.000 claims description 32
- 235000013601 eggs Nutrition 0.000 claims description 28
- 235000012054 meals Nutrition 0.000 claims description 17
- 241000819999 Nymphes Species 0.000 claims description 15
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- 230000017448 oviposition Effects 0.000 abstract description 16
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Classifications
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- A01K67/033—
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- Catching Or Destruction (AREA)
- Housing For Livestock And Birds (AREA)
Abstract
The invention discloses an indoor insectary and culture method for thrips hawaiiensis. The indoor insectary for the thrips hawaiiensis comprises a cylindrical insect placing chamber and a frustum-shaped insectary. The cylindrical insect placing chamber comprises an upper-layer cover body and a lower-layer cover body. The upper-layer cover body and the lower-layer cover body are connected through a push-pull rod. Annular air holes are formed in the upper-layer cover body. A rubber ring is closely arranged in the tube wall of the insect placing chamber. The lower end of the frustum-shaped insectary is a base plate, and the upper end of the frustum-shaped insectary is the upper-layer cover body. The frustum-shaped insectary comprises a cover body connected through the push-pull rod. The cover body is arranged on the lower-layer cover body. The cover body is provided with a food tray. The food tray is contained with food needed by the thrips hawaiiensis. The cover body is provided with an oviposition tray. The oviposition tray is provided with an oviposition thin film and water. The cover body is provided with moisturizing filter paper. The invention further relates to a method of using an indoor culture pipe for the thrips hawaiiensis to culture the thrips hawaiiensis. The indoor insectary for the thrips hawaiiensis has the advantages that the structure is simple, the production is simple and convenient, a special-assigned person is not needed to guard, the culture method guarantees that the survival rate and hatching rate are high in the culturing process, the escaping quantity of the thrips hawaiiensis is reduced, and at the same time the survival rate and the oviposition quantity of the thrips hawaiiensis are improved.
Description
Method field
The invention belongs to agricultural methods field, particularly relate to a kind of yellow chest thrips indoor feeding method.
Background method
Yellow chest thrips Thrips hamaiiensis (Morgan) belongs to thrips Thripidae, has another name called Thrips hawaiinensis, is the important pests of various melon, fruit and vegetables bud and young fruit of causing harm.This worm is mainly distributed in South Asia, Southeast Asia, East Asia Region, economizes on (district) tropical crop all have generation in Chinese Hainan, Guangdong, Guangxi, Yunnan, Taiwan, Fujian etc., and breaks out serious in recent years on Hainan Province's melon dish fruit.Yellow chest thrips generation more than 1 year, generation overlap, the tender tissue of plant of whenever causing harm in 1 year.Yellow chest thrips is taken out in flower bud petal plant and causes harm, the bud of plant is once extract out, and this worm assembles at once in groups, is invaded by petal shoulder crack, file inhales ovary and the fruitlet juice of plant, after bud bract opens, yellow chest thrips migrate into immediately do not open bract bud or tender leaf in, continue to cause harm, cause pericarp tissue hyperplasia, suberification, be projection pore (blackspot) to the fruit later stage, have a strong impact on fruit appearance quality, reduce economic worth; Ovum is produced in the young leaves do not launched simultaneously, after egg hatching, take food in young leaves, cause blade to turn white and growth arrest, cause and cause harm, after mounted blade, occur two symmetrical shrinkages at Ye Zhongmai, cause leaf malformation, withered.Yellow chest thrips pupates in soil, usually hiddenly in bud or flower flower bud bract, brings certain difficulty to preventing and controlling, not prophylactico-therapeutic measures safely and effectively at present.As can be seen here, yellow chest thrips has a strong impact on the seed output and quality of tropical crop, is current China hot-zone, especially the insect of most danger on tropical fruit (tree) and melon dish, how effectively to prevent and treat thrips to cause harm, become the important topic that China's hot-zone anti-season melon dish industry needs solution badly.
Yellow chest thrips fertility is very strong, and individual small, have invisible, field control is difficult to effective control.Under the equilibrium temperature of torrid areas, within 1 year, can to recur more than ten from generation to generation, the bisexual reproduction of female worm row and parthenogenesis, on common host plant, blastoprolepsis, and fertility is extremely strong.Yellow chest thrips is spread at a distance and mainly relies on human factor, the allocation and transportation of seedling, seed, flowers and other agricultural product, and especially manually carrying is the major way of its long-distance communications, and its survival ability is strong.So rely on single prevention and control measure to be difficult to obtain desirable control effects, physical control and biological control method is utilized to be the important channels solving the harm of yellow chest thrips.At present, the indoor feeding method of yellow chest thrips is difficult to solve, and can not meet a large amount of worm source, limit the further investigation of yellow chest thrips Synthetical prevention method.
In document record, there is not the report of the yellow chest thrips of indoor mass rearing, only have the method for some other thrips artificial feeding, comprise concave slide method for breeding, potted plant method for breeding, penicillin bottle method for breeding.(1) concave slide method for breeding is involutory together with two panels concave slide, makes concave surface form a closed space, form small-sized feeder, inside put fresh blade or pollen, take food and moisturizing for thrips.To be this method for breeding close two pieces of concave surfaces its advantage of concave slide method for breeding and form a closed environment, thrips escape can be prevented, its shortcoming is that space is little, in concave slide, air cannot circulate, and is unfavorable for that thrips survives wherein, vigor and survival rate lower, in addition, when changing food, thrips easily takes advantage of the occasion to run away, unfavorable operation; (2) one end rubber band of cloche is first pricked last layer gauze by potted plant method for breeding, the other end inserts in potted plant soil, plants the host plant being implanted with thrips in cloche, obtains thrips adult in land for growing field crops, move into the thrips adult needing to raise with test tube, raise in cloche.Its advantage of potted plant method for breeding is access expansion condition, and can obtain the yellow chest thrips that a large amount of vigor is high, its shortcoming is that thrips very easily escapes, thrips lays eggs not easily collecting, unfavorable operation, the extremely small and generation overlap of another thrips nymph, is difficult to obtain worm consistent, that vigor is higher polypide in age and studies; (3) penicillin bottle method for breeding is being similar to penicillin bottle middle berth thin layer cotton, cotton covers filter paper, add suitable quantity of water, prick last layer gauze with rubber band and seal bottleneck, put into thrips ovum and fresh bud in bottle, on filter paper, add suitable quantity of water with syringe, put into porcelain dish, raise in 28 DEG C of climatic cabinates, changed bud every 2-3 days.The activity space that its advantage of penicillin bottle method for breeding is available to thrips is large, and bud is easily fresh-keeping, and the survival rate of thrips is high, and shortcoming is not easy to operate, when especially changing food, take away sealing gauze thrips and be easy to escape, and in every bottle, foster worm amount is little.Yellow chest thrips be that it grows needs pollen addicted to flower property thrips, and in concave slide method for breeding and penicillin bottle method for breeding, breeding facility is too small, and pollen and filter paper are put into wherein, and do not separate, pollen is easily moist, causes thrips not take food; Potted plant raising rule is grasped because host's plant blossom phase is very difficult, and required cloche is also large, and yellow chest thrips is small due to build, very easily escapes or death, and therefore, above-mentioned 3 kinds of methods are all not suitable for indoor feeding Huang chest thrips.
Method problem to be solved by this invention be to provide a kind of raising method simple, prevent yellow chest thrips from escaping, easily exchange food for, easily collecting ovum grain, easy to use, indoor can the novel foster worm device of mass rearing yellow chest thrips adult and raising method.
Summary of the invention
The object of this invention is to provide the indoor insectary of a kind of yellow chest thrips adult and raising method, grow the yellow chest thrips neat, vigor is higher to realize obtaining in a large number in indoor.
The present invention discloses a kind of yellow chest thrips indoor feeding pipe, comprising:
Cylindrically put zooecium (10),
Upper strata lid (8) and lower floor's lid (4), described upper strata lid (8) is connected by pull bar (2) with described lower floor lid (4),
Described upper strata lid (8) is arranged the air-vent (9) of annular,
Elastic tape (7) be close to be placed on described in put in the tube wall of zooecium (10),
Taper type insectary (3), lower end is base plate (1), upper end is described upper strata lid (8), comprise: the lid (5) connected by pull bar (2), described lid (5) is arranged on described lid (4), described lid (5) is provided with meal tray (11), in described meal tray (11), places the food (12) needed for thrips
Described lid (5) is provided with nesting tray (13), described nesting tray (13) is provided with film of laying eggs (14) and water (15),
Described lid (5) is provided with moisturizing filter paper (6).
Preferably, the tapering of described taper type insectary (3) is between 40 ~ 60 °, and outer surface covers skid resistant course.
Preferably, described upper strata lid (8) is circular lid, thickness between 1-3 mm, and described upper strata lid (8) outer rim is put in zooecium (10) described in being placed on by described elastic tape (7), and described bottom (1) diameter is 1.5 ~ 2 times of described upper strata lid (8).
Preferably, described meal tray (11) to be diameter be 6-10mm, be highly the cylinder open plastic casing of 3-5mm, by two-sided gluing on described lid (5);
Described nesting tray (13) to be diameter be 6-10mm, be highly the cylinder open plastic casing of 3-5mm, by two-sided gluing on described lid (5);
Described film of laying eggs (14) is close to the edge of described nesting tray (13), be diameter is 10-15mm, thickness is the circular sealed membrane of 0.03-0.05mm,
The circular filter paper of described moisturizing filter paper (6) to be diameter be 0.75-4cm, is placed on described nesting tray (13) after folding.
Preferably, described upper strata lid (8) and described lid (5) are fixed on described pull bar (2), put the length of zooecium (10) described in the distance between described upper strata lid (8) and described lid (5) is greater than.
Preferably, the described elastic tape (7) of described upper strata lid (8) outer rim is made up of sealing material, prevents thrips escape.
The present invention also provides the raising method of a kind of yellow chest thrips, uses the yellow chest thrips adult indoor feeding pipe according to any one of claim 1-6 to carry out, comprises the steps:
The raising of adult: insectary (3) are pulled open by pull bar (2), the pollen of 10-20mg is added in meal tray (11), at moisturizing filter paper (6) upper instillation 5-10ml clear water, yellow chest thrips adult 20-30 head is moved in insectary (3), described pull bar (2) is pushed into described meal tray (11) position of described insectary (3), upper strata lid (8) stays the position of putting zooecium (10), then described insectary (3) is placed in climate box;
The hatching of worm's ovum: have film of laying eggs (14) on the nesting tray (13) of inside, the clear water of instillation 1-2ml, to lay eggs described in yellow chest thrips can puncture with tail bone film (14), produced in water by ovum, be then poured on gauze by water, worm's ovum is in gauze surface, worm's ovum is transferred in foster worm ware, then rest worm ware and be placed in climate box, treat egg hatching, after spending 4-6 days, egg hatching obtains nymph;
The raising of nymph: the nymph supporting gained in worm ware is transferred to described insectary (3), the pollen of 10-20mg is added in described meal tray (11), at described moisturizing filter paper (6) upper instillation 5-10ml clear water, described insectary (3) are placed in climate box, 1 age nymph white, polypide is small, 2 age nymph milk yellow, obviously movable, the yellow chest prepupal period of thrips and pseudo-pupa time no longer take food, now in climate box, reduce light application time, be beneficial to and pupate and turn into adult.
Preferably, adult and larva 1-2 raise stage climatic cabinate and be set to temperature 28 DEG C ± 2 DEG C, relative moisture 75% ± 7% length of times, and the photoperiod is L: D=16: 8; Egg hatching stage relative moisture 28 DEG C ± 2 DEG C, 75% ± 7%, the photoperiod is L: D=4: 20, and larva nymph prepupal period and pseudo-pupa time raise stage relative moisture 28 DEG C ± 2 DEG C, 75% ± 7%, and the photoperiod is L: D=6: 18.
The present invention has following beneficial effect:
Device simple structure of the present invention, easy to manufacture, be applicable to raising yellow chest thrips, be particularly suitable for yellow chest thrips and take food and lay eggs in same space, and be used for studying its life habit, for anti-chest thrips harm of harnessing the Yellow River provides important theoretical foundation, in addition, use this device easily to change thrips food, yellow chest thrips can be allowed fully to take food pollen and obtain nutrition, conveniently can take out the ovum grain of yellow chest thrips, accurately to draw spawning rate simultaneously; Moreover utilize this device spawning efficiency high, the egg laying amount of every 30 yellow chest thrips can reach 1400-2000 grain, the simultaneously hatching of ovum or survival rate also improve greatly, can reach 80-90%.
Accompanying drawing explanation
Fig. 1 is yellow chest thrips indoor feeding pipe perspective view;
Fig. 2 is the perspective view of meal tray;
Fig. 3 is the perspective view of nesting tray;
Fig. 4 is the culture dish perspective view of ovum grain hatching;
In figure: 1, bottom; 2, pull bar; 3, taper type insectary; 4, lid; 5, lid; 6, moisturizing filter paper; 7, elastic tape; 8, upper strata lid; 9, air-vent; 10, cylindrically zooecium is put; 11, meal tray; 12, food; 13, nesting tray; 14, to lay eggs film; 15, water; 16, culture dish lid; 17, filter screen; 18, culture dish.
Embodiment
By reference to the accompanying drawings the specific embodiment of the present invention is described, the indoor feeding pipe of a kind of thrips, device, comprising:
A kind of yellow chest thrips indoor feeding pipe, is characterized in that, comprising:
Cylindrically put zooecium 10,
Upper strata lid 8 and lower floor's lid 4, described upper strata lid 8 is connected by pull bar 2 with described lower floor lid 4,
Described upper strata lid 8 is arranged the air-vent 9 of annular,
Elastic tape 7 be close to be placed on described in put in the tube wall of zooecium 10,
Taper type insectary 3, lower end is base plate 1, and upper end is described upper strata lid 8, comprise: the lid 5 connected by pull bar 2, described lid 5 is arranged on described lid 4, and described lid 5 is provided with meal tray 11, the food 12 needed for thrips is placed in described meal tray 11
Described lid 5 is provided with nesting tray 13, described nesting tray 13 is provided with lay eggs film 14 and water 15,
Described lid 5 is provided with moisturizing filter paper 6.
The tapering of described taper type insectary 3 is between 40 ~ 60 °, and outer surface covers skid resistant course.
Described upper strata lid 8 is circular lid, thickness between 1-3 mm, and described upper strata lid 8 outer rim be placed on by described elastic tape 7 described in put in zooecium 10, described bottom 1 diameter is 1.5 ~ 2 times of described upper strata lid 8.
Described meal tray 11 to be diameters be 6-10mm, be highly the cylinder open plastic casing of 3-5mm, by two-sided gluing on described lid 5;
Described nesting tray 13 to be diameters be 6-10mm, be highly the cylinder open plastic casing of 3-5mm, by two-sided gluing on described lid 5;
Described film 14 of laying eggs is close to the edge of described nesting tray 13, be diameter is 10-15mm, thickness is the circular sealed membrane of 0.03-0.05mm,
The circular filter paper of described moisturizing filter paper 6 to be diameters be 0.75-4cm, is placed on described nesting tray 13 after folding.
Described upper strata lid 8 and described lid 5 are fixed on described pull bar 2, put the length of zooecium 10 described in the distance between described upper strata lid 8 and described lid 5 is greater than.
The described elastic tape 7 of described upper strata lid 8 outer rim is made up of sealing material, prevents thrips escape.
Assembling insectary time, the upper chamber end of thread is allowed to be inserted in lower cavity, make two cavity compact siro spinning technology as a whole, this assembling advantage shown in Fig. 1 is, the environment of climatic cabinate can be put into after assembling, the optimal environmental condition that yellow chest thrips grows and lays eggs can be provided, after Adult worms producting eggs terminates, easily lower floor's cavity can be taken out, observation is laid eggs situation and can regulate or change condition of culture at any time and to be allowed to condition under optimum condition growth and to lay eggs, after having assembled cavity, pass through pull bar, lid 5 is first pushed into lower floor's cavity, lid 4 stays upper strata cavity.Upper strata lid arranges some allows gas can carry out the inside and outside aperture exchanged, the diameter of aperture is 0.1-0.2mm, the number of aperture is above 10, preferably symmetrical, be conducive to air circulation like this, when this insectary is inserted in artificial environment, thrips also can well live, and improves time and the efficiency of Adult worms producting eggs.Nesting tray is bonded on lower floor's lid by double faced adhesive tape, can facilitate and change fresh water at any time, avoid being placed on for a long time film produces and go mouldy, thus damaging effect is produced to the ovum of output, can also reuse in addition and not need the device that at every turn more renews.In addition, upper strata cavity and lower floor's cavity are transparent lucite, the living condition of the yellow chest thrips in so easy observation cavity.One or several above in these reasons may be exactly allow device of laying eggs of the present invention have the reasonable dismissal of reasonable effect.
Rearing conditions
In order to further illustrate effect of the present invention, We conducted test, these tests are only enumerating of limited embodiment of the present invention, do not form the restrictive explanation of the claims in the present invention.
1, the selection of the quantity of insectary Neihuang County chest thrips is put into
Test method: test sets 4 groups to be respectively in each insectary as 10,30,50 and 70 yellow chest thrips adults, process 5 repetitions, be positioned in climatic cabinate, set temperature 28 DEG C ± 1 DEG C, humidity 75% ± 5%, photoperiod is L: D=10: 14, Continuous Observation 3 days, the active situation of yellow chest thrips adult is observed by timing, within 3rd day, take out double-deck lid by pull bar, confirm the death condition of yellow chest thrips adult with writing brush, take out nesting tray simultaneously, examine under a microscope egg laying amount.
Result of the test: the statistics of the 3rd day is in table 1, known: under putting into 4 kinds of yellow chest thrips quantity gradients of insectary, yellow chest thrips adult all has mate and oviposit, but the process lethality of the 3rd day putting into 30 adults in insectary is starkly lower than other 3 process, the egg laying amount of adult is apparently higher than other 3 process (table 1) simultaneously.
The different impact on adult mortality and egg laying amount of insectary quantity put into by table 1
2, yellow chest thrips is to the selection of temperature
Test method: point 2 tests, under test (1) establishes 5 groups to be respectively 20 DEG C, 24 DEG C, 28,32 DEG C and 36 DEG C of Temperature Treatment, often process yellow chest thrips adult 30 and put into insectary, repeat for 5 times, be positioned in climatic cabinate, humidity 75% ± 5% is set, photoperiod is L: D=10: 14, Continuous Observation 3 days, observed the active situation of yellow chest thrips adult by timing, within the 3rd day, take out double-deck lid by pull bar, confirm the death condition of yellow chest thrips adult with writing brush, take out nesting tray simultaneously, examine under a microscope egg laying amount; Under test (2) establishes 5 groups to be respectively 20 DEG C, 24 DEG C, 28,32 DEG C and 36 DEG C of Temperature Treatment, often process yellow 30, chest thrips ovum and put into culture dish, repeat for 5 times, humidity 75% ± 5% is set, photoperiod is L: D=6: 18, Continuous Observation 10 days, records the phase of going through of ovum grain hatching in each culture dish and calculates incubation rate, the phase (d) of on average going through of ovum and incubation rate (P) computational methods of ovum are respectively: d=Σ (d ＇ × N ＇)/Σ N ＇, P=N
hatching nymph/ N
egg laying amount, d ＇ is number of days after laying eggs, and N ＇ is the ovum amount of hatching in this time period.
Result of the test: the result of test (1), the statistics of the 3rd day is in table 2, known: under putting into 5 kinds of thermogrades of insectary, yellow chest thrips adult all has mate and oviposit, but 28 DEG C of process lethality of the 3rd day is starkly lower than other 4 process, the egg laying amount of adult is apparently higher than other 4 process (table 2) simultaneously; The result of test (2), after 10 days, statistics is in table 2, known: under putting into 5 kinds of thermogrades of culture dish, yellow chest thrips ovum grain all has hatching, but 28 DEG C of process are on average gone through time phase and are starkly lower than other 4 process, the incubation rate of ovum processes (table 3) apparently higher than other 4 simultaneously
Table 2 yellow chest thrips adult lethality at different temperatures and egg laying amount
Table 3 yellow chest thrips ovum phase of on average going through at different temperatures and incubation rate
3, yellow chest thrips is to the selection of light application time
Test method: point 2 tests, test (1) establishes 4 groups to be respectively under 12:12,10:14,8:16 and 6:18 photoperiod processes, often process yellow chest thrips adult 30 and put into insectary, repeat for 5 times, be positioned in climatic cabinate, set temperature 28 DEG C ± 1 DEG C, humidity 75% ± 5%, Continuous Observation 3 days, observed the active situation of yellow chest thrips adult by timing, within the 3rd day, take out double-deck lid by pull bar, confirm the death condition of yellow chest thrips adult with writing brush, take out nesting tray simultaneously, examine under a microscope egg laying amount; Test (2) establishes 4 groups to be respectively under 10:14,8:16,6:18 and 4:20 photoperiod processes, often process yellow 30, chest thrips ovum and put into culture dish, repeat for 5 times, set temperature 28 DEG C ± 1 DEG C, humidity 75% ± 5%, Continuous Observation 10 days, records the phase of going through of ovum grain hatching in each culture dish and calculates incubation rate, the phase (d) of on average going through of ovum and incubation rate (P) computational methods of ovum are respectively as d=Σ (d ＇ × N ＇)/Σ N ＇, P=N
hatching nymph/ N
egg laying amount, d ＇ is number of days after laying eggs, and N ＇ is the ovum amount of hatching in this time period.
Result of the test: the result of test (1), the statistics of the 3rd day is in table 2, known: under putting into 4 kinds of photoperiod gradients of insectary, yellow chest thrips adult all has mate and oviposit, but the lethality that the photoperiod arranges under 10:14 photoperiod set handling the 3rd day is starkly lower than other 3 process, and the egg laying amount of adult is apparently higher than other 3 process (table 4) simultaneously; The result of test (2), after 10 days, statistics is in table 2, known: under putting into 4 kinds of photoperiod gradients of culture dish, yellow chest thrips ovum grain all has hatching, but time phase of on average going through of ovum is starkly lower than other 3 process under 6:18 photoperiod set handling, the incubation rate of ovum is apparently higher than other 3 process (table 5) simultaneously
The lethality of table 4 yellow chest thrips adult under the different light time and egg laying amount
The go through phase of table 5 yellow chest thrips ovum under the different light time and incubation rate
Claims (8)
1. a yellow chest thrips indoor feeding pipe, is characterized in that, comprising:
Cylindrically put zooecium (10),
Upper strata lid (8) and lower floor's lid (4), described upper strata lid (8) is connected by pull bar (2) with described lower floor lid (4),
Described upper strata lid (8) is arranged the air-vent (9) of annular,
Elastic tape (7) be close to be placed on described in put in the tube wall of zooecium (10),
Taper type insectary (3), lower end is base plate (1), upper end is described upper strata lid (8), comprise: the lid (5) connected by pull bar (2), described lid (5) is arranged on described lid (4), described lid (5) is provided with meal tray (11), in described meal tray (11), places the food (12) needed for thrips
Described lid (5) is provided with nesting tray (13), described nesting tray (13) is provided with film of laying eggs (14) and water (15),
Described lid (5) is provided with moisturizing filter paper (6).
2. yellow chest thrips adult indoor feeding pipe according to claim 1, is characterized in that, the tapering of described taper type insectary (3) is between 40 ~ 60 °, and outer surface covers skid resistant course.
3. yellow chest thrips adult indoor feeding pipe according to claim 1, it is characterized in that, described upper strata lid (8) is circular lid, thickness between 1-3 mm, and described upper strata lid (8) outer rim is put in zooecium (10) described in being placed on by described elastic tape (7), and described bottom (1) diameter is 1.5 ~ 2 times of described upper strata lid (8).
4. yellow chest thrips adult indoor feeding pipe according to claim 1, it is characterized in that, described meal tray (11) to be diameter be 6-10mm, be highly the cylinder open plastic casing of 3-5mm, by two-sided gluing on described lid (5);
Described nesting tray (13) to be diameter be 6-10mm, be highly the cylinder open plastic casing of 3-5mm, by two-sided gluing on described lid (5);
Described film of laying eggs (14) is close to the edge of described nesting tray (13), be diameter is 10-15mm, thickness is the circular sealed membrane of 0.03-0.05mm,
The circular filter paper of described moisturizing filter paper (6) to be diameter be 0.75-4cm, is placed on described nesting tray (13) after folding.
5. yellow chest thrips adult indoor feeding pipe according to claim 1, it is characterized in that, described upper strata lid (8) and described lid (5) are fixed on described pull bar (2), put the length of zooecium (10) described in the distance between described upper strata lid (8) and described lid (5) is greater than.
6. yellow chest thrips adult indoor feeding pipe according to claim 1, it is characterized in that, the described elastic tape (7) of described upper strata lid (8) outer rim is made up of sealing material, prevents thrips escape.
7. a raising method for yellow chest thrips, is characterized in that, uses the yellow chest thrips adult indoor feeding pipe according to any one of claim 1-6 to carry out, comprises the steps:
The raising of adult: insectary (3) are pulled open by pull bar (2), the pollen of 10-20mg is added in meal tray (11), at moisturizing filter paper (6) upper instillation 5-10ml clear water, yellow chest thrips adult 20-30 head is moved in insectary (3), described pull bar (2) is pushed into described meal tray (11) position of described insectary (3), upper strata lid (8) stays the position of putting zooecium (10), then described insectary (3) is placed in climate box;
The hatching of worm's ovum: have film of laying eggs (14) on the nesting tray (13) of inside, the clear water of instillation 1-2ml, to lay eggs described in yellow chest thrips can puncture with tail bone film (14), produced in water by ovum, be then poured on gauze by water, worm's ovum is in gauze surface, worm's ovum is transferred in foster worm ware, then rest worm ware and be placed in climate box, treat egg hatching, after spending 4-6 days, egg hatching obtains nymph;
The raising of nymph: the nymph supporting gained in worm ware is transferred to described insectary (3), the pollen of 10-20mg is added in described meal tray (11), at described moisturizing filter paper (6) upper instillation 5-10ml clear water, described insectary (3) are placed in climate box, 1 age nymph white, polypide is small, 2 age nymph milk yellow, obviously movable, the yellow chest prepupal period of thrips and pseudo-pupa time no longer take food, now in climate box, reduce light application time, be beneficial to and pupate and turn into adult.
8. raising method according to claim 7, is characterized in that, adult and larva 1-2 raise stage climatic cabinate and be set to temperature 28 DEG C ± 2 DEG C, relative moisture 75% ± 7% length of time, and the photoperiod is L: D=16: 8; Egg hatching stage relative moisture 28 DEG C ± 2 DEG C, 75% ± 7%, the photoperiod is L: D=4: 20, and larva nymph prepupal period and pseudo-pupa time raise stage relative moisture 28 DEG C ± 2 DEG C, 75% ± 7%, and the photoperiod is L: D=6: 18.
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409887A (en) * | 2015-10-26 | 2016-03-23 | 山东省农业科学院植物保护研究所 | Breeding method for thrips palmi karny |
| CN107410217A (en) * | 2017-05-16 | 2017-12-01 | 中国热带农业科学院环境与植物保护研究所 | The breeding apparatus and method of cassava monodactyle mite experimental population |
| CN111328770A (en) * | 2020-03-25 | 2020-06-26 | 广西壮族自治区农业科学院 | Indoor large-scale breeding method for nakayata nakamura |
| CN111466344A (en) * | 2020-04-23 | 2020-07-31 | 广西大学 | Method for continuously feeding thrips |
| CN113273547A (en) * | 2021-05-24 | 2021-08-20 | 吉林农业大学 | Breeding device and breeding method for cynara scolymus |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2865276Y (en) * | 2005-12-22 | 2007-02-07 | 云南农业大学 | Thripid insects indoor feeding pot |
| CN101731183A (en) * | 2008-11-27 | 2010-06-16 | 甘肃农业大学 | Odontothrips loti feeding method |
| CN102524188A (en) * | 2012-01-20 | 2012-07-04 | 福建省农业科学院植物保护研究所 | Insect breeding bottom and breeding method of gynaikothrips ficorum |
| CN102550493A (en) * | 2012-01-17 | 2012-07-11 | 甘肃农业大学 | Method for artificial infestation of Odontothrips loti on alfalfa |
| CN203120742U (en) * | 2013-02-25 | 2013-08-14 | 吉林农业大学 | Egg parasitoid natural enemy insect breeding box with internal grid support |
| CN203435557U (en) * | 2013-09-16 | 2014-02-19 | 安徽省农业科学院植物保护与农产品质量安全研究所 | Circular-truncated-cone spawning cage for insects |
| CN204272975U (en) * | 2014-12-04 | 2015-04-22 | 广西大学 | Multi-functional retractable dependent insect cage |
| CN105284747A (en) * | 2015-11-30 | 2016-02-03 | 广西大学 | Method and device for indoor breeding of phauda flammans walker |
-
2015
- 2015-05-26 CN CN201510273796.2A patent/CN104872068B/en not_active Expired - Fee Related
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2865276Y (en) * | 2005-12-22 | 2007-02-07 | 云南农业大学 | Thripid insects indoor feeding pot |
| CN101731183A (en) * | 2008-11-27 | 2010-06-16 | 甘肃农业大学 | Odontothrips loti feeding method |
| CN102550493A (en) * | 2012-01-17 | 2012-07-11 | 甘肃农业大学 | Method for artificial infestation of Odontothrips loti on alfalfa |
| CN102524188A (en) * | 2012-01-20 | 2012-07-04 | 福建省农业科学院植物保护研究所 | Insect breeding bottom and breeding method of gynaikothrips ficorum |
| CN203120742U (en) * | 2013-02-25 | 2013-08-14 | 吉林农业大学 | Egg parasitoid natural enemy insect breeding box with internal grid support |
| CN203435557U (en) * | 2013-09-16 | 2014-02-19 | 安徽省农业科学院植物保护与农产品质量安全研究所 | Circular-truncated-cone spawning cage for insects |
| CN204272975U (en) * | 2014-12-04 | 2015-04-22 | 广西大学 | Multi-functional retractable dependent insect cage |
| CN105284747A (en) * | 2015-11-30 | 2016-02-03 | 广西大学 | Method and device for indoor breeding of phauda flammans walker |
Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105409887A (en) * | 2015-10-26 | 2016-03-23 | 山东省农业科学院植物保护研究所 | Breeding method for thrips palmi karny |
| CN105409887B (en) * | 2015-10-26 | 2018-01-12 | 张志鹏 | A kind of method for breeding of palm thrips |
| CN107410217A (en) * | 2017-05-16 | 2017-12-01 | 中国热带农业科学院环境与植物保护研究所 | The breeding apparatus and method of cassava monodactyle mite experimental population |
| CN107410217B (en) * | 2017-05-16 | 2023-06-06 | 中国热带农业科学院环境与植物保护研究所 | Breeding device and method for experimental population of cassava single-claw mites |
| CN111328770A (en) * | 2020-03-25 | 2020-06-26 | 广西壮族自治区农业科学院 | Indoor large-scale breeding method for nakayata nakamura |
| CN111328770B (en) * | 2020-03-25 | 2021-10-22 | 广西壮族自治区农业科学院 | Indoor large-scale breeding method for nakayata nakamura |
| CN111466344A (en) * | 2020-04-23 | 2020-07-31 | 广西大学 | Method for continuously feeding thrips |
| CN113273547A (en) * | 2021-05-24 | 2021-08-20 | 吉林农业大学 | Breeding device and breeding method for cynara scolymus |
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