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CN104817349A - Preparation method of zinc-iron-rich special biological organic fertilizer for fresh eating corn - Google Patents

Preparation method of zinc-iron-rich special biological organic fertilizer for fresh eating corn Download PDF

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Publication number
CN104817349A
CN104817349A CN201510144241.8A CN201510144241A CN104817349A CN 104817349 A CN104817349 A CN 104817349A CN 201510144241 A CN201510144241 A CN 201510144241A CN 104817349 A CN104817349 A CN 104817349A
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iron
zinc
fertilizer
earthworm
rich
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吴志刚
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Suzhou Rencheng Bio-Science & Technology Co Ltd
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Suzhou Rencheng Bio-Science & Technology Co Ltd
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    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/20Fertilizers of biological origin, e.g. guano or fertilizers made from animal corpses

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Abstract

The invention discloses a preparation method of a zinc-iron-rich special biological organic fertilizer for fresh eating corn. The method comprises the following steps: 1, preparing a zinc-iron-rich cow and sheep dung material; 2, preparing a zinc-iron-rich earthworm manure fertilizer; 3, mixing the zinc-iron-rich cow and sheep dung material and the zinc-iron-rich earthworm manure fertilizer with needle mushroom bacterial residue, continuously adding Bacillus megaterium, Bacillus subtilis, Aspergillus niger and Streptomyces albogriseolus, and carrying out secondary fermentation to obtain a mixed fermented material; and 5, air-drying the mixed fermented material, air-drying, crushing, and carrying out packaging preservation to obtain the zinc-iron-rich special biological organic fertilizer for fresh eating corn. The method allows inorganic zinc and iron to be converted into zinc and iron forms beneficial for plant absorption and utilization through multi-time fermentation by animal stomach microbes and in vitro added microbes; fresh eating corn cultivated by applying the biological organic fertilizer prepared through the method has high content of zinc and iron, and good mouthfeel quality; and the biological organic fertilizer improves the economic benefit of plantation of the fresh eating corn, and deserves to be widely popularized.

Description

The preparation method of the special rich zinc-iron biological organic fertilizer of a kind of fresh edible maize
Technical field:
The present invention relates to agricultural fertilizer field, particularly relate to a kind of preparation method of biological organic fertilizer, be specially the preparation method of the special rich zinc-iron biological organic fertilizer of a kind of fresh edible maize.
Background technology:
Zinc (Zn) is the necessary natural element of growth and development of plants and animals, the existence of zinc is had in the rock of most of earth's crust, average content is about 70mg/kg, average content in the outer field litre geosphere of the earth's crust, pedosphere and biosphere is about 50mg/kg, and in hydrosphere, the contents level of zinc is all lower is about 0.01mg/kg.All there is the situation of scarce zinc in the part soil of the calcareous soil of the northern area of China and the saline soil of coastland and southern area, in the area that some scarce zinc are serious, the shortage of ZINC IN SOIL element has become the principal element limiting local Agriculture Production.
People will take in the zinc element of 15-25mg every day by food, body just can be made not lack zinc, and many areas find to lack zinc in food chain by research, can be serious affect the healthy of local resident especially teenager and children.The zinc of needed by human body is mainly derived from plant from soil, absorbs the zinc obtained.Due in soil and crop in lack zinc and cause the output of crop reduce and quality decline phenomenon worldwide very general, the shortage of ZINC IN SOIL oneself become the restricted nutritional factor of crop growth, had a strong impact on the yield and quality of crop.
American science research committee is recommended, and people needs the amount taking in zinc to be 15mg at 11 years old to adulthood every day, and in the meals that Chinese NNFA is recommended, every day needs the amount supplying zinc to be also 15mg.According to the diet formula of Chinese residents, grownup takes in the amount of zinc at about lOmg every day, if that is the zinc content in food crop seed is brought up to original 2-3 doubly, just can ensure that intake reaches 15mg every day.3 kinds of approach are had: one is that cultivate can the staple food crop such as crop varieties, particularly wheat, paddy rice, corn of gathering trace nutritive element to food supplement trace nutritive element; Two is the materials adding certain micronutrient element or be rich in certain trace element in food; Three is use the fertilizer being rich in some micronutrient element, and combines with crop varieties, produces so-called functional foodstuff.First method length consuming time, input are also comparatively large, and also may involve transgenosis problem, what second method was supplemented is external source zinc, and the third method is more feasible way for current, has had some successful experiences at home and abroad.In recent years, a few thing had done in rich selenium, Fu Zi and Fu Tie rice and wheat in China, and what have applies in production fairly largely.
Corn is the important food crop of China, and its cultivated area and output are only second to paddy rice and wheat, occupies the 3rd.Corn is C4 plant, and the dry that respiration consumes is few, and photosynthetic efficiency is higher, and thus yield per unit is higher, and yield per unit occupies first of world food crop.Fresh edible maize is also Green maize, namely refers to the corn ear of milk stage, removing bract and fringe handle, and boiling is to ripe ready-to-serve corn.Fresh edible maize is a concept corresponding with the dry corn dewatered after complete ripeness, and its feature is: not dewatering of milk stage results, processing or unprocessed, for edible corn.Fresh edible maize can be conventional corn, sweet corn, Glutinous Semen Maydis, high nutrient corn and characteristic odor type corn, is good especially with sweet corn, Glutinous Semen Maydis.Sweet corn skin is thin sweet tender, and the fragrant glutinous exquisiteness of Glutinous Semen Maydis meat, likes by people very much.
Fresh edible maize nutritive ingredient and edibleness all go even farther compared with the dry seed of the corn gathered in the crops after complete ripeness and fabricated product.According to eating the sweet type, glutinous type, odor type and the fresh corn of coventional type four class that require to gather raw, various nutritive ingredient is all obviously better than the dry seed of conventional corn.Fresh edible maize contains multiple amino acids, trace element, lipid acid etc., and nutrient categories reaches tens kinds.Specific value obtain it is noted that in the dry seed of conventional corn starch content very high, considerably reduce in fresh edible maize; In dry seed, the protein of inferior quality, is improved; The VITAMIN that nutritive value is quite high, mineral substance zinc-iron selenium and Mierocrystalline cellulose nutritive ingredient, in fresh edible maize, content is all significantly increased.
Chinese invention patent CN104186150A discloses a kind of cultivating method of rich selenium zinc-iron corn, in the soil of maize planting, rich selenium zinc-iron chelating fertilizer is applied heading stage at corn, described rich selenium zinc-iron chelating fertilizer contains chelating selenium salt, chelate zinc salt and chelating molysite, the sequestrant of described chelating selenium salt is amino acid, and the sequestrant of described chelate zinc salt and chelating molysite is ethylenediamine tetraacetic acid (EDTA); The amount of application of described rich selenium zinc-iron chelating fertilizer is in the selenium in chelating fertilizer, zinc, ferro element, and the amount of application of selenium element is 0.8 ~ 1.5 gram/acre, and the amount of application of zinc element is 80 ~ 150 grams/acre, and the amount of application of ferro element is 150 ~ 200 grams/acre.This invention also applies corn composite fertilizer while the rich selenium zinc-iron chelating fertilizer of applying, effectively can improve the content of ferroselenium zinc element in corn kernel, but the absorption rate of this invention ferroselenium zinc element is on the low side, and is unfavorable for improving soil quality, causes environmental pollution.
Chinese invention patent CN103787772A discloses the agent of a kind of corn zinc-rich nutrient and preparation thereof and the method for the production of rich zinc corn.The zinc-rich nutrient agent of this corn, is made up of the component of following parts by weight: zinc source, with zinc weighing scale 0.5 ~ 25.5; Biological organic fertilizer 3.5 ~ 6.2; Acidity regulator 2.08 ~ 4.3; Fertilizer binding agent 0.01 ~ 0.05.In corn planting process, apply the agent of above-mentioned corn zinc-rich nutrient.The corn zinc content that the method provided according to this invention is produced can reach 15.7 ~ 30.5mg/kg, is more than the twice of conventional corn Zn content.The corn that this invention is produced meets the requirement of state food standard, maintain the object that the original nutrition of corn reaches again rich zinc, there is good physiologically active, be easy to digest and assimilate, but the object that the present invention improves corn zinc content is still undertaken by adding Inorganic Zn fertilizer, well can not improve the absorption of zinc fertilizer, and long-term application increases the weight of ground pressure, be unfavorable for the improvement of ecological environment of soil.
Summary of the invention:
The present invention is directed to prior art Problems existing, the object of the present invention is to provide the preparation method of the special rich zinc-iron biological organic fertilizer of a kind of fresh edible maize, use the inventive method and prepare biological organic fertilizer, cultivate the fresh edible maize zinc iron content obtained high, taste quality is good, improve the economic benefit of fresh edible maize plantation, improve soil quality simultaneously, there is great promotional value.
The invention provides following technical scheme:
A preparation method for the special rich zinc-iron biological organic fertilizer of fresh edible maize, preparation process is:
The first step: prepare rich zinc-iron cattle and sheep ight soil material;
Second step: prepare rich zinc-iron earthworm night soil;
3rd step: add bacillus megaterium after being mixed with rich zinc-iron earthworm night soil and needle mushroom dreg by rich zinc-iron cattle and sheep ight soil material, subtilis, black-koji mould, white light gray streptomycete carry out Secondary Fermentation, obtained mixed fermentation material;
4th step: air-dry for mixed fermentation material pulverizing, pack preservation are obtained the special rich zinc-iron biological organic fertilizer of fresh edible maize.
Preferably, rich zinc-iron cattle and sheep ight soil material in the described the first step is that cattle and sheep Dietary Supplemented Zinc is fertile and iron fertilizer, in Zn zinc fertilizer addition for 250mg/kg, in the addition of Fe iron fertilizer for 200mg/kg, under collecting the forage feed of this interpolation zinc fertilizer and iron fertilizer, obtain cattle and sheep ight soil material.
Preferably, the preparation process of described second step rich zinc-iron earthworm night soil is;
A. chicken manure is dried rear interpolation zinc fertilizer and iron fertilizer, in Zn zinc fertilizer addition for 250mg/kg, in the addition of Fe iron fertilizer for 200mg/kg, mix and regulate moisture content to be 60-65%, obtained earthworm breeding material;
B. be respectively the fine sand of the cement pit bottom paving thick layer 1cm of 1m × 1m × 0.5m in length, width and height, a fine sand surface paving gauze, then adds earthworm breeding material 100kg described in step A, puts earthworm breeding pond in order;
C. rearing pool inoculates earthworm 1.8-2.2 kilogram after putting in order every other day, and earthworm kind loves earthworm (Eisenia fetida) for compatriot;
D. earthworm faecal samples was collected every ten days, air-dry obtained earthworm rich iron zinc ight soil material after being completed by earthworm ight soil sample collection.
Preferably, according to parts by weight, rear continuation interpolation bacillus megaterium, subtilis, black-koji mould, white light gray streptomycete after 150-180 part richness zinc-iron cattle and sheep ight soil material, 7-10 richness zinc-iron earthworm night soil, the mixing of 50-75 needle mushroom dreg are carried out Secondary Fermentation in described 3rd step, the addition of bacterial classification is respectively 0.2%, 0.3%, 0.2% of rich zinc-iron cattle and sheep ight soil material, rich zinc-iron earthworm night soil and needle mushroom dreg three mixture quality, leavening temperature is 30-32 DEG C, fermentation time is 2-3 days, and ferment complete obtained mixed fermentation material.
Preferably, in described 4th step by air-dry for mixed fermentation material be 15-18% to moisture content, pulverized 40 mesh sieves, pack preserved.
Preferably, the addition of described interpolation bacillus megaterium, subtilis, black-koji mould, white light gray streptomycete all with living bacteria count gauge, living bacteria count amount >=500,000,000/gram.
The feature of biological organic fertilizer: 1 improves chemical fertilizer utilization ratio, and biological organic fertilizer has the effects such as fixed nitrogen, phosphorus decomposing, potassium decomposing, greatly can improve the utilization ratio of chemical fertilizer.2 improvement soil, increase soil fertility, in biological organic fertilizer, not only organic component content enriches, in addition also containing be conducive to edatope improve probiotics.After soil application biological organic fertilizer, add the content of the nutritive substances such as the organic matter of soil, nitrogen, phosphorus, potassium to a great extent, phosphorus decomposing in fertilizer, potassium solubilizing bacteria play its phosphorus decomposing, potassium decomposing effect in soil, phosphoric in soil and potassium element are discharged, adds the available content of mineral substances of plant in soil.On the other hand, because biological organic fertilizer is applied in soil, add the organic content in soil, make that soil becomes loose, nutrient laden, the original flora lived in soil obtains amount reproduction, thus ensure that the micro-ecological environment of soil.3 alleviate environmental pollution, and according to statistics, have 1.4 hundred million tons to be burned in the agricultural crop straw of more than 600,000,000 ton that China produces every year, the burning of stalk not only causes the waste of organic substance, also creates atmospheric pollution.These discarded stalks are used and are fermented into biological organic fertilizer, huge economic benefit and the protection of air will be produced to agricultural.Use biological organic fertilizer can improve soil quality and soil microenvironment.4 improve crop quality, and the multiple nutritional components that biological organic fertilizer is rich in imparts the special fertilizer efficiency of fertilizer, proves after deliberation, and biological organic fertilizer can improve color and luster, the quality such as mouthfeel and growth vigor of crop.5 alleviate crop pests, and one is the bacteriostatic action of biological organic fertilizer self, and two is effect of biological organic fertilizer on crops growth, antibacterial, disease-resistant promoter action.Research finds the increase along with soil with organic matter content, and the validity of ZINC IN SOIL also increases thereupon.
The trophism of zinc to plant mainly contains: (1) zinc is the component of many enzymes, and zinc affects the activity of tens of kinds of enzymes.(2) participate in photosynthesis and respiration, zinc is one of moiety of chloroplast(id), plays an important role to chlorophyllous formation and function.(3) promote protein metabolism, zinc is the component of the necessary RNA polymerase of protein synthesis, and an obvious characteristic of plant zinc deficiency is exactly rna polymerase activity raising in plant.(4) promote reproductive development and improve resistance, zinc has impact to reproductive development and fertilization.
Beneficial effect of the present invention:
1. traditional zinc fertilizer and iron fertilizer transform and are more conducive to plant absorption and utilize by the inventive method after the multiple-microorganism of animal gastrointestinal microbiological and external interpolation repeatedly ferments, use the inventive method and prepare biological organic fertilizer, cultivate the fresh edible maize zinc iron content obtained high, taste quality is good, improves the economic benefit of fresh edible maize plantation.
2. the present invention contains multiple effective microbe, retain the good function characteristic that general fertilizer has, can promote corn to nutritive element absorb and tangerine resists the pathogenic effects of some pathogenic micro-organism, alleviate the disease and pest in corn planting process, improve plantation quality and output, improve economy and zoology benifit.
3. the inventive method to prepare in biological organic fertilizer the content that abundant zinc-iron element not only can improve zinc-iron element in corn kernel after corn planting, improve the economic benefit of fresh edible maize, and zinc-iron element has good promoter action for growth and development of plants, milpa photosynthesis and respiration, promotion protein metabolism can be promoted, promote reproductive development and improve resistance, there is high economic worth.
Embodiment:
Be described in detail embodiments of the invention below, the present embodiment is implemented under premised on invention technical scheme, give detailed embodiment and concrete operating process, but protection scope of the present invention is not limited to following embodiment.The experimental program of unreceipted actual conditions in embodiment, the condition that conveniently condition or manufacturers advise usually is implemented.
Embodiment one
A preparation method for the special rich zinc-iron biological organic fertilizer of fresh edible maize, preparation process is:
The first step: prepare rich zinc-iron cattle and sheep ight soil material.
By fertile for cattle and sheep Dietary Supplemented Zinc zinc chloride and iron fertilizer ferrous sulfate, in Zn zinc fertilizer addition for 250mg/kg, in the addition of Fe iron fertilizer for 200mg/kg, under collecting the forage feed of this interpolation zinc fertilizer and iron fertilizer, obtain cattle and sheep ight soil, air-dryly obtain rich zinc-iron ight soil material.
Second step: prepare rich zinc-iron earthworm night soil.
A. chicken manure is dried rear interpolation zinc fertilizer zinc chloride and iron fertilizer ferrous sulfate, in Zn zinc fertilizer addition for 250mg/kg, in the addition of Fe iron fertilizer for 200mg/kg, mix and regulate moisture content to be 60%, obtained earthworm breeding material;
B. be respectively the fine sand of the cement pit bottom paving thick layer 1cm of 1m × 1m × 0.5m in length, width and height, a fine sand surface paving gauze, then adds earthworm breeding material 100kg described in step A, puts earthworm breeding pond in order;
C. rearing pool inoculates earthworm 1.8 kilograms every other day after putting in order, and earthworm kind loves earthworm (Eisenia fetida) for compatriot;
D. earthworm faecal samples was collected every ten days, air-dry obtained earthworm rich iron zinc ight soil material after being completed by earthworm ight soil sample collection.
3rd step: continuation interpolation bacillus megaterium rear after 150 parts of rich zinc-iron cattle and sheep ight soil material, 7 rich zinc-iron earthworm night soils, 50 needle mushroom dreg mixing, subtilis, black-koji mould, white light gray streptomycete are carried out Secondary Fermentation according to parts by weight, the addition of bacterial classification is respectively 0.2%, 0.3%, 0.2% of rich zinc-iron cattle and sheep ight soil material, rich zinc-iron earthworm night soil and needle mushroom dreg three mixture quality, leavening temperature is 30 DEG C, fermentation time is 2 days, and ferment complete obtained mixed fermentation material;
4th step: by air-dry for mixed fermentation material be 15% to moisture content, pulverized 40 mesh sieves, pack preserved.
Embodiment two
A preparation method for the special rich zinc-iron biological organic fertilizer of fresh edible maize, preparation process is:
The first step: prepare rich zinc-iron cattle and sheep ight soil material.
By fertile for cattle and sheep Dietary Supplemented Zinc zinc chloride and iron fertilizer ferrous sulfate, in Zn zinc fertilizer addition for 250mg/kg, in the addition of Fe iron fertilizer for 200mg/kg, under collecting the forage feed of this interpolation zinc fertilizer and iron fertilizer, obtain cattle and sheep ight soil, air-dryly obtain rich zinc-iron cattle and sheep ight soil material.
Second step: prepare rich zinc-iron earthworm night soil.
A. chicken manure is dried rear interpolation zinc fertilizer zinc chloride and iron fertilizer ferrous sulfate, in Zn zinc fertilizer addition for 250mg/kg, in the addition of Fe iron fertilizer for 200mg/kg, mix and regulate moisture content to be 65%, obtained earthworm breeding material;
B. be respectively the fine sand of the cement pit bottom paving thick layer 1cm of 1m × 1m × 0.5m in length, width and height, a fine sand surface paving gauze, then adds earthworm breeding material 100kg described in step A, puts earthworm breeding pond in order;
C. rearing pool inoculates earthworm 2.2 kilograms every other day after putting in order, and earthworm kind loves earthworm (Eisenia fetida) for compatriot;
D. earthworm faecal samples was collected every ten days, air-dry obtained earthworm rich iron zinc ight soil material after being completed by earthworm ight soil sample collection.
3rd step: continuation interpolation bacillus megaterium rear after 180 parts of rich zinc-iron cattle and sheep ight soil material, 10 rich zinc-iron earthworm night soils, 75 needle mushroom dreg mixing, subtilis, black-koji mould, white light gray streptomycete are carried out Secondary Fermentation according to parts by weight, the addition of bacterial classification is respectively 0.2%, 0.3%, 0.2% of rich zinc-iron cattle and sheep ight soil material, rich zinc-iron earthworm night soil and needle mushroom dreg three mixture quality, leavening temperature is 32 DEG C, fermentation time is 3 days, and ferment complete obtained mixed fermentation material;
4th step: by air-dry for mixed fermentation material be 18% to moisture content, pulverized 40 mesh sieves, pack preserved.
Embodiment three
A preparation method for the special rich zinc-iron biological organic fertilizer of fresh edible maize, preparation process is:
The first step: prepare rich zinc-iron cattle and sheep ight soil material.
By fertile for cattle and sheep Dietary Supplemented Zinc zinc oxide and iron fertilizer ferric sulfate, in Zn zinc fertilizer addition for 250mg/kg, in the addition of Fe iron fertilizer for 200mg/kg, under collecting the forage feed of this interpolation zinc fertilizer and iron fertilizer, obtain cattle and sheep ight soil, air-dryly obtain rich zinc-iron cattle and sheep ight soil material.
Second step: prepare rich zinc-iron earthworm night soil;
A. chicken manure is dried rear interpolation zinc fertilizer zinc oxide and iron fertilizer ferric sulfate, in Zn zinc fertilizer addition for 250mg/kg, in the addition of Fe iron fertilizer for 200mg/kg, mix and regulate moisture content to be 63%, obtained earthworm breeding material;
B. be respectively the fine sand of the cement pit bottom paving thick layer 1cm of 1m × 1m × 0.5m in length, width and height, a fine sand surface paving gauze, then adds earthworm breeding material 100kg described in step A, puts earthworm breeding pond in order;
C. rearing pool inoculates earthworm 2 kilograms every other day after putting in order, and earthworm kind loves earthworm (Eiseniafetida) for compatriot;
D. earthworm faecal samples was collected every ten days, air-dry obtained earthworm rich iron zinc ight soil material after being completed by earthworm ight soil sample collection.
3rd step: continuation interpolation bacillus megaterium rear after 170 parts of rich zinc-iron cattle and sheep ight soil material, 9 rich zinc-iron earthworm night soils, 60 needle mushroom dreg mixing, subtilis, black-koji mould, white light gray streptomycete are carried out Secondary Fermentation according to parts by weight, the addition of bacterial classification is respectively 0.2%, 0.3%, 0.2% of rich zinc-iron cattle and sheep ight soil material, rich zinc-iron earthworm night soil and needle mushroom dreg three mixture quality, leavening temperature is 30 DEG C, fermentation time is 2 days, and ferment complete obtained mixed fermentation material;
4th step: by air-dry for mixed fermentation material be 16% to moisture content, pulverized 40 mesh sieves, pack preserved.
Embodiment four
A preparation method for the special rich zinc-iron biological organic fertilizer of fresh edible maize, preparation process is:
The first step: prepare rich zinc-iron cattle and sheep ight soil material.
By fertile for cattle and sheep Dietary Supplemented Zinc zinc oxide and iron fertilizer ferric sulfate, in Zn zinc fertilizer addition for 250mg/kg, in the addition of Fe iron fertilizer for 200mg/kg, under collecting the forage feed of this interpolation zinc fertilizer and iron fertilizer, obtain cattle and sheep ight soil, air-dryly obtain rich zinc-iron cattle and sheep ight soil material.
Second step: prepare rich zinc-iron earthworm night soil;
A. chicken manure is dried rear interpolation zinc fertilizer zinc oxide and iron fertilizer ferric sulfate, in Zn zinc fertilizer addition for 250mg/kg, in the addition of Fe iron fertilizer for 200mg/kg, mix and regulate moisture content to be 62%, obtained earthworm breeding material;
B. be respectively the fine sand of the cement pit bottom paving thick layer 1cm of 1m × 1m × 0.5m in length, width and height, a fine sand surface paving gauze, then adds earthworm breeding material 100kg described in step A, puts earthworm breeding pond in order;
C. rearing pool inoculates earthworm 1.9 kilograms every other day after putting in order, and earthworm kind loves earthworm (Eisenia fetida) for compatriot;
D. earthworm faecal samples was collected every ten days, air-dry obtained earthworm rich iron zinc ight soil material after being completed by earthworm ight soil sample collection.
3rd step: continuation interpolation bacillus megaterium rear after 165 parts of rich zinc-iron cattle and sheep ight soil material, 8 rich zinc-iron earthworm night soils, 59 needle mushroom dreg mixing, subtilis, black-koji mould, white light gray streptomycete are carried out Secondary Fermentation according to parts by weight, the addition of bacterial classification is respectively 0.2%, 0.3%, 0.2% of rich zinc-iron cattle and sheep ight soil material, rich zinc-iron earthworm night soil and needle mushroom dreg three mixture quality, leavening temperature is 32 DEG C, fermentation time is 2 days, and ferment complete obtained mixed fermentation material;
4th step: by air-dry for mixed fermentation material be 16% to moisture content, pulverized 40 mesh sieves, pack preserved.
Add described in embodiment one to four bacillus megaterium, subtilis, black-koji mould, white light gray streptomycete addition all with living bacteria count gauge, number of viable >=500,000,000/gram.Use according to described specification sheets using method after purchase, addition is converted into living bacteria count amount to be added, the bacillus megaterium viable bacteria content such as bought be 80-100 hundred million/gram, then its addition is according to the 500000000/gram conversion of claim number of viable, is bacillus megaterium according to 1/16th of the required grams of 500,000,000/gram calculating.
Zinc, iron level adopt Microwave Digestion to carry out pre-treatment, and inductive coupling plasma emission spectrograph (ICP-AES) measures, and result represents with dry weight.
Planting experiment
Experiment place: three villages of Xi'an City, Shanxi Province, are denoted as village A, B, C respectively.
Corn variety: conventional corn kind Zheng Dan 958 (ZD958).
For examination soil: the physico-chemical property in soil, three villages is in table one.
A piece of land is chosen in each village, be divided into 18 fritters, every three pieces divide one group at random, be divided into into 6 groups, wherein use preparation method described in embodiment one to four respectively and prepare the special rich zinc-iron biological organic fertilizer of fresh edible maize for four pieces, be denoted as treatment group one to four group, amount of application is 50kg/ mu, use the corn bio-feritlizer that market is bought for one piece, amount of application is used according to purchase bio-feritlizer specification sheets, be denoted as process one, last block uses the bio-feritlizer bought on the market described in process one and buys the zinc fertilizer and iron fertilizer that add on the market, usage quantity and using method are referring to specification sheets.
Adopt general planting mode and way to manage.
Milk stage, gathers fresh fringe, and survey zinc-iron constituent content in corn yield and fresh fringe, statistics is in table two and table three table four.
Table one: village ABC soil physico-chemical property
Physico-chemical property Village A Village B Village C
pH(H 2O) 8.20 7.36 7.30
Organic (g/kg) 10.6 14.5 16.2
Total nitrogen (g/kg) 0.79 0.86 0.95
Available phosphorus (mg/kg) 12.6 8.4 9.5
Effective potassium (mg/kg) 95 114 127
Total zinc (mg/kg) 65.21 83.21 75.48
Effective zinc (mg/kg) 0.48 0.79 1.45
Total iron (g/kg) 2.03 1.87 2.31
Effective iron (mg/kg) 5.69 6.85 7.31
Data are all expressed in the mode of mean number ± variance.
Table two: planting experiment corn yield (units/kg/mu)
Village A Village B Village C
Treatment group one 665.8±35.6 669.8±39.5 613.5±39.4
Treatment group two 659.8±36.5 672.5±29.8 608.9±42.6
Treatment group three 663.2±30.7 654.2±27.3 621.5±44.8
Treatment group four 648.5±29.8 649.8±30.5 625.8±49.5
Control group one 621.4±30.8 620.1±33.9 604.1±35.8
Control group two 635.1±40.8 625.8±34.8 610.2±36.9
Data are all expressed in the mode of mean number ± variance.
Table three: zinc element content (unit mg/kg) in fresh fringe
Village A Village B Village C
Treatment group one 21.08±3.36 16.69±6.01 18.92±3.64
Treatment group two 20.92±3.28 17.28±4.52 19.53±5.01
Treatment group three 22.61±3.04 15.94±5.28 17.69±3.87
Treatment group four 25.73±4.28 16.57±5.27 18.64±4.05
Control group one 10.34±2.51 9.24±3.07 6.38±1.28
Control group two 16.89±3.67 13.26±5.39 13.54±4.09
Data are all expressed in the mode of mean number ± variance.
Table four: iron content (unit mg/kg) in fresh fringe
Village A Village B Village C
Treatment group one 13.08±2.05 11.41±2.58 18.76±3.17
Treatment group two 14.21±3.04 12.54±2.67 19.24±3.38
Treatment group three 13.69±3.33 11.29±3.64 20.03±2.95
Treatment group four 13.58±2.69 10.58±3.15 18.52±4.41
Control group one 6.38±2.01 3.67±2.28 6.68±2.40
Control group two 10.25±3.36 8.59±3.01 13.22±6.14
Data are all expressed in the mode of mean number ± variance.
According to table two to table four, we can find out that the special rich zinc-iron biological organic fertilizer of fresh edible maize that preparation method of the present invention prepares effectively can improve the content of zinc-iron element in fresh edible maize seed, and output comparatively other biological fertilizer also improve.
Above content is only better embodiment of the present invention, and for those of ordinary skill in the art, according to thought of the present invention, all will change in specific embodiments and applications, this description should not be construed as limitation of the present invention.

Claims (6)

1. a preparation method for the special rich zinc-iron biological organic fertilizer of fresh edible maize, it is characterized in that, preparation process is:
The first step: prepare rich zinc-iron cattle and sheep ight soil material;
Second step: prepare rich zinc-iron earthworm night soil;
3rd step: add bacillus megaterium after being mixed with rich zinc-iron earthworm night soil and needle mushroom dreg by rich zinc-iron cattle and sheep ight soil material, subtilis, black-koji mould, white light gray streptomycete carry out Secondary Fermentation, obtained mixed fermentation material;
4th step: air-dry for mixed fermentation material pulverizing, pack preservation are obtained the special rich zinc-iron biological organic fertilizer of fresh edible maize.
2. the preparation method of the special rich zinc-iron biological organic fertilizer of a kind of fresh edible maize according to claim 1, it is characterized in that: rich zinc-iron cattle and sheep ight soil material in the described the first step, it is that cattle and sheep Dietary Supplemented Zinc is fertile and iron fertilizer, in Zn zinc fertilizer addition for 250mg/kg, in Fe iron fertilizer addition for 200mg/kg, under collecting the forage feed of this interpolation zinc fertilizer and iron fertilizer, obtain cattle and sheep ight soil material.
3. the preparation method of the special rich zinc-iron biological organic fertilizer of a kind of fresh edible maize according to claim 1, is characterized in that: the preparation process of described second step rich zinc-iron earthworm night soil is;
A. chicken manure is dried rear interpolation zinc fertilizer and iron fertilizer, in Zn zinc fertilizer addition for 250mg/kg, in the addition of Fe iron fertilizer for 200mg/kg, mix and regulate moisture content to be 60-65%, obtained earthworm breeding material;
B. be respectively the fine sand of the cement pit bottom paving thick layer 1cm of 1m × 1m × 0.5m in length, width and height, a fine sand surface paving gauze, then adds earthworm breeding material 100kg described in step A, puts earthworm breeding pond in order;
C. rearing pool inoculates earthworm 1.8-2.2 kilogram after putting in order every other day, and earthworm kind loves earthworm (Eisenia fetida) for compatriot;
D. earthworm faecal samples was collected every ten days, air-dry obtained earthworm rich iron zinc ight soil material after being completed by earthworm ight soil sample collection.
4. the preparation method of the special rich zinc-iron biological organic fertilizer of a kind of fresh edible maize according to claim 1, it is characterized in that: in described 3rd step according to parts by weight by rich for 150-180 part zinc-iron cattle and sheep ight soil material, 7-10 rich zinc-iron earthworm night soil, continue to add bacillus megaterium after after the mixing of 50-75 needle mushroom dreg, subtilis, black-koji mould, white light gray streptomycete carries out Secondary Fermentation, the addition of bacterial classification is respectively rich zinc-iron cattle and sheep ight soil material, 0.2% of rich zinc-iron earthworm night soil and needle mushroom dreg three mixture quality, 0.3%, 0.2%, leavening temperature is 30-32 DEG C, fermentation time is 2-3 days, ferment complete obtained mixed fermentation material.
5. the preparation method of the special rich zinc-iron biological organic fertilizer of a kind of fresh edible maize according to claim 1, is characterized in that: in described 4th step by air-dry for mixed fermentation material be 15-18% to moisture content, pulverized 40 mesh sieves, pack preserved.
6. the preparation method of the special rich zinc-iron biological organic fertilizer of a kind of fresh edible maize according to claim 1, it is characterized in that: the addition of described interpolation bacillus megaterium, subtilis, black-koji mould, white light gray streptomycete all with living bacteria count gauge, living bacteria count amount >=500,000,000/gram.
CN201510144241.8A 2015-03-30 2015-03-30 Preparation method of zinc-iron-rich special biological organic fertilizer for fresh eating corn Pending CN104817349A (en)

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CN108220208A (en) * 2018-03-16 2018-06-29 青岛农业大学 A kind of zinc-rich bacterial strain and its application
CN108299081A (en) * 2018-02-02 2018-07-20 邓龙飞 A kind of microbial function fertilizer and preparation method thereof
CN110759778A (en) * 2019-11-25 2020-02-07 覃建忠 Zinc-selenium biological activating enzyme liquid phosphorus dissolving agent and preparation method thereof

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CN104261919A (en) * 2014-10-10 2015-01-07 青岛嘉瑞生物技术有限公司 Process for producing efficient bioorganic fertilizers by resource utilization of dairy cow manure
CN104355791A (en) * 2014-10-20 2015-02-18 合肥长润农业科技有限公司 Novel compound coated fertilizer with stable fertilizer efficiency and preparation method thereof
CN104380961A (en) * 2014-10-30 2015-03-04 灵璧县林汇家禽育种有限责任公司 Planting method for selenium-enriched maize

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CN104261919A (en) * 2014-10-10 2015-01-07 青岛嘉瑞生物技术有限公司 Process for producing efficient bioorganic fertilizers by resource utilization of dairy cow manure
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CN108299081A (en) * 2018-02-02 2018-07-20 邓龙飞 A kind of microbial function fertilizer and preparation method thereof
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