CN104777369A - Method for measuring conductivity of suspension liquid after plasma sterilization - Google Patents
Method for measuring conductivity of suspension liquid after plasma sterilization Download PDFInfo
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- CN104777369A CN104777369A CN201510217940.0A CN201510217940A CN104777369A CN 104777369 A CN104777369 A CN 104777369A CN 201510217940 A CN201510217940 A CN 201510217940A CN 104777369 A CN104777369 A CN 104777369A
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- liquid
- conductivity
- measured
- suspension liquid
- supernatant
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- 239000007788 liquid Substances 0.000 title claims abstract description 21
- 238000000034 method Methods 0.000 title claims abstract description 11
- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 11
- 230000001954 sterilising effect Effects 0.000 title claims abstract description 10
- 239000000725 suspension Substances 0.000 title claims abstract description 9
- 239000006228 supernatant Substances 0.000 claims abstract description 10
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 4
- 239000000523 sample Substances 0.000 claims description 6
- WFJIVOKAWHGMBH-UHFFFAOYSA-N 4-hexylbenzene-1,3-diol Chemical compound CCCCCCC1=CC=C(O)C=C1O WFJIVOKAWHGMBH-UHFFFAOYSA-N 0.000 claims description 4
- 239000003550 marker Substances 0.000 claims description 3
- 230000001580 bacterial effect Effects 0.000 abstract description 10
- 210000000170 cell membrane Anatomy 0.000 abstract description 5
- 230000035699 permeability Effects 0.000 abstract description 5
- 238000005119 centrifugation Methods 0.000 abstract 1
- 239000008223 sterile water Substances 0.000 abstract 1
- CWHJIJJSDGEHNS-MYLFLSLOSA-N Senegenin Chemical compound C1[C@H](O)[C@H](O)[C@@](C)(C(O)=O)[C@@H]2CC[C@@]3(C)C(CC[C@]4(CCC(C[C@H]44)(C)C)C(O)=O)=C4[C@@H](CCl)C[C@@H]3[C@]21C CWHJIJJSDGEHNS-MYLFLSLOSA-N 0.000 description 3
- 150000002500 ions Chemical class 0.000 description 3
- 239000009871 tenuigenin Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 241000588724 Escherichia coli Species 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 230000001086 cytosolic effect Effects 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 230000007773 growth pattern Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 238000003786 synthesis reaction Methods 0.000 description 1
Landscapes
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
The invention discloses a method for measuring the conductivity of suspension liquid after plasma sterilization. The method comprises the steps that firstly, a biofilm block treated through plasma is placed into a 50 ml sterile spiral-mouth centrifuge tube loaded with 10 ml of sterile water, so liquid to be measured is obtained; afterwards, centrifugation is performed on the liquid to be measured in a centrifuge, so supernatant liquid is obtained, and then the conductivity is measured through a portable conductometer. According to the method, the permeability change and rupture conditions of a cell membrane are indirectly detected by detecting the change of the conductivity of bacterial suspension liquid.
Description
Technical field
The invention belongs to disinfection technology field, in particular, provide a kind of method measuring plasma sterilization rear suspension liquid conductivity.
Background technology
Tenuigenin is the environment of bacterium, is the place of protein, various enzyme and Nucleic acid, is also the place of carrying out the metabolism of material synthesis Sum decomposition after absorbing nutriment.In tenuigenin, containing a large amount of various ion to maintain self vital movement and acid base equilibrium.Once bacterial cell membrane permeability changes and breaks, these ions will escape into extracellular along with tenuigenin, thus bacterial suspension intermediate ion concentration is increased.So indirect detection can be carried out to permeability of cell membrane change and rupture event by the change of conductivity in bacterial detection suspending liquid.Plasma sterilization rear suspension liquid conductivity variations law study is very few both at home and abroad at present.
Summary of the invention
For solving the problem, the present invention proposes a kind of method measuring plasma sterilization rear suspension liquid conductivity.
The present invention, for reaching above object, is realized by following technical scheme:
The step comprised is as follows: (1), revolve putting into the 50ml aseptic, 10ml sterilized water is housed through plasma treated mycoderm film block in mouthful centrifuge tube, notices that each centrifuge tube is corresponding and carries out marker number, obtain liquid to be measured; (2), by liquid to be measured carry out centrifugal treating in centrifuges and obtain supernatant, under then inserting supernatant liquid level with the probe of portable conductivity meter, notice that probe is soaked in after in supernatant completely and start to measure, read after stable reading is stable.
Accompanying drawing explanation
Fig. 1 is conductivity variations and sterilization effect figure.
Embodiment
Embodiment
In superclean bench, preparation is containing strain Escherichia coli (numbering: the mycoderm film block of 1 × 1cm ATTC25922) is for subsequent use.
(1), select air micro arc plasma body jet as plasma-generating source, the mycoderm film block plasma treated through 0s, 10s, 15s, 30s, 45s, 60s, 90s is put into the 50ml aseptic, 10ml sterilized water is housed respectively to be revolved in mouthful centrifuge tube, notice that each centrifuge tube is corresponding and carry out marker number, obtain liquid to be measured;
(2), liquid to be measured is carried out centrifugal treating (4000r in centrifuges, 10min) obtain supernatant, then under inserting supernatant liquid level with the probe of portable conductivity meter (DDS-307), notice that probe is soaked in after in supernatant completely to start to measure, read after stable reading is stable.Sterilization rate, as accompanying drawing 1, is analyzed and can be found out by the experimental result obtained together with conductivity, and 0-10s is the stage that bacterial death is maximum, bacterial death rate reaches 80%, and conductivity now raises trend obviously, increase to 3.08us/cm from the 2.37us/cm of 0s, lift-off value reaches 0.71us/cm.In the process of 10-30s, the growth pattern of bacterial death number also change that is basic and conductivity is consistent, and this phase bacterial mortality ratio is elevated to about 97% from 80%, and conductivity is now also corresponding increases 0.36us/cm.And in after 30s one minute, bacterial death number increasess slowly, and conductivity now also keeps stable, changes hardly.It can thus be appreciated that, the change of bacterium sterilization rate keeps basically identical with the change of conductivity, exactly because and the change bacterial cell membrane permeability of conductivity changes and causes cytoplasmic effusion, this also illustrates in action of plasma process, and colibacillary permeability of cell membrane there occurs change.
Claims (1)
1. one kind measures the method for plasma sterilization rear suspension liquid conductivity, it is characterized in that the step of the method is as follows: (1), revolve in mouthful centrifuge tube by putting into the 50ml aseptic, 10ml sterilized water is housed through plasma treated mycoderm film block, notice that each centrifuge tube is corresponding and carry out marker number, obtain liquid to be measured; (2), by liquid to be measured carry out centrifugal treating in centrifuges and obtain supernatant, under then inserting supernatant liquid level with the probe of portable conductivity meter, notice that probe is soaked in after in supernatant completely and start to measure, read after stable reading is stable.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510217940.0A CN104777369A (en) | 2015-04-24 | 2015-04-24 | Method for measuring conductivity of suspension liquid after plasma sterilization |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510217940.0A CN104777369A (en) | 2015-04-24 | 2015-04-24 | Method for measuring conductivity of suspension liquid after plasma sterilization |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN104777369A true CN104777369A (en) | 2015-07-15 |
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| Application Number | Title | Priority Date | Filing Date |
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| CN201510217940.0A Pending CN104777369A (en) | 2015-04-24 | 2015-04-24 | Method for measuring conductivity of suspension liquid after plasma sterilization |
Country Status (1)
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| CN (1) | CN104777369A (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108956705A (en) * | 2018-08-24 | 2018-12-07 | 内蒙古仁创沙产业有限公司 | A kind of inorganic binder sand conductivity measuring method |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1846130A (en) * | 2003-08-06 | 2006-10-11 | 布里杰技术有限公司 | Bridged element for detection of a target substance |
| WO2007104163A1 (en) * | 2006-03-16 | 2007-09-20 | Al-Amin Dhirani | Dielectric sensing method and system |
| CN101231257A (en) * | 2008-02-19 | 2008-07-30 | 东南大学 | Chip and method for testing biological corpuscle dielectric property |
| CN102680654A (en) * | 2011-12-19 | 2012-09-19 | 河南科技大学 | Method for building model for collectively evaluating bioavailability and toxicity of cadmium accumulation in food for human body |
| CN104120097A (en) * | 2014-06-24 | 2014-10-29 | 中山大学 | Escherichia coli culture method for quantitative sterilization research |
-
2015
- 2015-04-24 CN CN201510217940.0A patent/CN104777369A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1846130A (en) * | 2003-08-06 | 2006-10-11 | 布里杰技术有限公司 | Bridged element for detection of a target substance |
| WO2007104163A1 (en) * | 2006-03-16 | 2007-09-20 | Al-Amin Dhirani | Dielectric sensing method and system |
| CN101231257A (en) * | 2008-02-19 | 2008-07-30 | 东南大学 | Chip and method for testing biological corpuscle dielectric property |
| CN102680654A (en) * | 2011-12-19 | 2012-09-19 | 河南科技大学 | Method for building model for collectively evaluating bioavailability and toxicity of cadmium accumulation in food for human body |
| CN104120097A (en) * | 2014-06-24 | 2014-10-29 | 中山大学 | Escherichia coli culture method for quantitative sterilization research |
Non-Patent Citations (2)
| Title |
|---|
| 徐洪斌 等: "高压静电杀菌灭藻技术研究", 《污染防治技术》 * |
| 成建宏 等: "种子的介电灭菌消毒与选分技术", 《中国种业》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108956705A (en) * | 2018-08-24 | 2018-12-07 | 内蒙古仁创沙产业有限公司 | A kind of inorganic binder sand conductivity measuring method |
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Application publication date: 20150715 |
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