CN104757111A - Compound probiotics fermented plant beverage and preparation method thereof - Google Patents
Compound probiotics fermented plant beverage and preparation method thereof Download PDFInfo
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- CN104757111A CN104757111A CN201510184888.3A CN201510184888A CN104757111A CN 104757111 A CN104757111 A CN 104757111A CN 201510184888 A CN201510184888 A CN 201510184888A CN 104757111 A CN104757111 A CN 104757111A
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Abstract
The invention provides a compound probiotics fermented plant beverage and a preparation method thereof. The preparation method comprises the following steps: firstly, preparing a direct adding type fermenting agent composed of two plants of probiotics including lactobacillus rhamnosus and lctobacillus casei; adding 1L of drinking water into 15g-20g of herba cirsii, 20g-25g of Chinese yams, 20g-30g of hawthorn fruits, 5g-10g of semen cannabis, 5g-10g of cassia seeds, 15g-20g of red dates, 15g-20g of wolfberry fruits, 10g-15g of poria cocos, 15g-20g of honeysuckles, 20g-30g of dandelions, 5g-10g of spina date seeds and 10g-20g of kudzu vine roots, and boiling to obtain an extracting solution; making the volume to be 1L by using the drinking water; adding fructo-oligose according to the ratio of 3.5g/L; and sterilizing at a high temperature, inoculating the direct adding type fermenting agent to ferment, and carrying out sterile filling to prepare the compound probiotics fermented plant beverage.
Description
(1) technical field
The present invention relates to a kind of composite probiotics ferment plant beverage and preparation method thereof, be specifically related to adopt the multiple edible extract of Chinese traditional medicine of composite probiotics ferment, and make a kind of there is active plant beverage of the effects such as fat-reducing, reducing blood lipid and preparation method thereof, belong to food processing field.
(2) background technology
Edible Chinese medicine is the food of seldom contact in people's daily life, often use in health food, but the price of health food is far away higher than bread and cheese, causes these edible medicinal materials with multiple efficacies to stop at general population, also limit the development of edible medicinal material.
Due to the raising of people's living standard, rhythm of life is accelerated, operating pressure increases increasingly, crowd of causeing fat increases erratic eating habit day by day, comprise person in middle and old age, teenager, even children, the symptom such as high fat of blood, hyperglycaemia caused because of fat has had a strong impact on the healthy of people.The various slim tea of the thing followed, slimming drugs arise at the historic moment, but these product majorities are only for relaxing bowel, can not long-term taking, return to again after stopping using and use front state, cannot fundamentally fat in metabolizing cells, cure the symptoms, not the disease, long-term taking also likely causes pharmacological dependence, has certain side effect to health.
Patent of the present invention adopts that multiple to have effect edible Chinese medicines such as lipopenicillinase, toxin expelling, antiviral, develop immunitypty be raw material, extract is produced after scientific compatibility, after composite probiotics ferment, the one of preparation has fat-reducing, develop immunitypty again, antitumor, the multiple efficacies such as delay senility, the applicable plant beverage drunk for a long time.
(3) summary of the invention
The object of the invention is to provide a kind of composite probiotics ferment plant beverage and preparation method thereof.
The object of the present invention is achieved like this: percentage involved in the present invention is mass ratio outside indicating, and product of the present invention adopts such method to prepare:
1. the selection of fermentation strain and the preparation of culture medium
Fermentation strain is purchased in Chinese industrial Microbiological Culture Collection administrative center, as follows respectively:
A bacterium---Lactobacillus rhamnosus, Lactobacillus rhamnosus, bacterial strain deposit number is:
B bacterium---lactobacillus paracasei, Lactobacillus paracasei, bacterial strain deposit number is:
A bacterium, B bacterium culture medium are: casein peptone 10.0g, beef extract 10.0g, dusty yeast 5.0g, glucose 5.0g, sodium acetate 5.0g, dibasic ammonium citrate 2.0g, Tween 801.0g, K
2hPO
42.0g, MgSO
4.7H
2o 0.2g, MnSO
1.H
2o 0.05g, CaCO
320.0g, distilled water 1.0L, pH6.8.
2. the preparation process of throw type leaven
The activation of 2.1 freeze-dried vaccine powder
Measure the physiological saline 10mL of 0.9% respectively in 2 in vitro, within 20 minutes, be cooled to 30 DEG C through 121 DEG C of sterilizings, all poured under aseptic conditions in the physiological saline of 0.9% by the freeze-dried vaccine powder in 2 strain bacterium ampullas, concussion makes it dissolve, in 30 DEG C of insulating boxs, static activation 30 minutes, for subsequent use.
2.2 expand cultivation
2.2.1 the preparation of mother culture
Measure each 200mL of 2 strain bacterium culture medium respectively in 2 500mL triangular flasks, 121 DEG C of sterilizings are cooled to 30 DEG C in 20 minutes, by activated bacterial classification in 5% inoculation 2.1 steps of culture volume, 30 DEG C of static gas wave refrigerator 24 hours, as mother culture.
2.2.2 the preparation of leavening is produced
First the defatted milk emulsion that mass percent is 10% is prepared, liquid glycerin, 2% xylo-oligosaccharide, 2% sucrose of 5% is added by defatted milk emulsion weight, 121 DEG C of sterilizings are cooled to 30 DEG C in 20 minutes, press defatted milk emulsion 5% volume ratio inoculation mother culture respectively, then 30 DEG C of static gas wave refrigerator 36 hours, detect 2 strain bacterium and produce leavening viable count, each production leavening viable count>=10
9individual/mL, treats as fermenting-ripening, if viable count < 10
9individual/mL, continues to cultivate, until reach 10
9individual/mL.
The preparation of 2.3 powder freeze-drying lactobacillus
The production leavening of maturation is aseptically imported in glass ampoule, liquid level 0.8-1cm, after adding a cover bottle stopper, put into-30 DEG C of refrigerator-freezer quick-frozens, by glass ampoule pallet splendid attire after freezing, put into freeze dryer and carry out freeze drying.
2.4 throw type leavens composite
By freeze-dried vaccine powder weight, get A bacterium powder 3-5 part, B bacterium powder 2-3 part, fully make throw type leaven after mixing, for compoiste fermented edible extract of Chinese traditional medicine, the addition of throw type leaven is 0.01% of compound edible extract of Chinese traditional medicine weight.
Need to further illustrate: the activation of the bacterial classification selected in the present invention, freeze drying process are not limited only to concrete grammar of the present invention, the composition of culture medium is also not limited thereto, other routine techniques and method are all fine, as long as can improve the vigor of bacterial classification and be prepared into lyophilized powder and easy to use.
3. the preparation of composite probiotics ferment plant beverage
3.1 edible Chinese medicine compatibilities
Field thistle 15-20g, Chinese yam 20-25g, hawthorn 20-30g, fructus cannabis 5-10g, cassia seed 5-10g, date 15-20g, fruit of Chinese wolfberry 15-20g, Poria cocos 10-15g, honeysuckle 15-20g, dandelion 20-30g, spina date seed 5-10g, root of kudzu vine 10-20g
3.2 extract preparations
The edible Chinese medicine of 3.1 step compatibilities is added 1L drinking water, and heating boils maintenance 5 minutes, filters, the filtered fluid drinking water of acquisition is settled to 1L and is extract after being cooled to 25 DEG C.
3.3 batching
Add FOS powder by extracting liquid volume, addition is 3.5g/L, stirs, and FOS is fully dissolved.
3.4 high-temperature sterilization
Heating material to the 121 DEG C sterilizing 3.3 steps prepared 20 minutes, is then cooled to 30 DEG C.
3.5 inoculum fermented
0.01% throw type leaven prepared of inoculation 2.4 steps of weight of material is prepared, 30 DEG C of static gas wave refrigerator 36 hours by 3.3 steps.
3.6 sterile filling
The zymotic fluid sterile filling 3.5 steps prepared, is composite probiotics ferment plant beverage.
The advantage of product of the present invention is to adopt composite probiotics ferment edible Chinese medicine extract, product is rich in Lactobacillus rhamnosus and lactobacillus paracasei, drink for a long time and be conducive to probio at intestinal colonisation, the scientific compatibility of edible medicinal material, the biological polyoses that plant polyose in leaching liquor and probiotics fermention produce organically combines, fat in tachymetabolism body cell, produce one strong immunization barrier simultaneously, make that product of the present invention has fat-reducing, develop immunitypty, antitumor, the multiple efficacies such as delay senility.
(4) detailed description of the invention
For a more detailed description to the present invention below in conjunction with specific embodiment:
Embodiment one:
1. the selection of fermentation strain and the preparation of culture medium
Fermentation strain is purchased in Chinese industrial Microbiological Culture Collection administrative center, as follows respectively:
A bacterium---Lactobacillus rhamnosus, Lactobacillus rhamnosus, bacterial strain deposit number is:
B bacterium---lactobacillus paracasei, Lactobacillus paracasei, bacterial strain deposit number is:
A bacterium, B bacterium culture medium are: casein peptone 10.0g, beef extract 10.0g, dusty yeast 5.0g, glucose 5.0g, sodium acetate 5.0g, dibasic ammonium citrate 2.0g, Tween 801.0g, K
2hPO
42.0g, MgSO
4.7H
2o 0.2g, MnSO
4.H
2o 0.05g, CaCO
320.0g, distilled water 1.0L, pH6.8.
2. the preparation process of throw type leaven
The activation of 2.1 freeze-dried vaccine powder
Measure the physiological saline 10mL of 0.9% respectively in 2 in vitro, within 20 minutes, be cooled to 30 DEG C through 121 DEG C of sterilizings, all poured under aseptic conditions in the physiological saline of 0.9% by the freeze-dried vaccine powder in 2 strain bacterium ampullas, concussion makes it dissolve, in 30 DEG C of insulating boxs, static activation 30 minutes, for subsequent use.
2.2 expand cultivation
2.2.1 the preparation of mother culture
Measure each 200mL of 2 strain bacterium culture medium respectively in 2 500mL triangular flasks, 121 DEG C of sterilizings are cooled to 30 DEG C in 20 minutes, by activated bacterial classification in 5% inoculation 2.1 steps of culture volume, 30 DEG C of static gas wave refrigerator 24 hours, as mother culture.
2.2.2 the preparation of leavening is produced
First the defatted milk emulsion that mass percent is 10% is prepared, liquid glycerin, 2% xylo-oligosaccharide, 2% sucrose of 5% is added by defatted milk emulsion weight, 121 DEG C of sterilizings are cooled to 30 DEG C in 20 minutes, press defatted milk emulsion 5% volume ratio inoculation mother culture respectively, then 30 DEG C of static gas wave refrigerator 36 hours, detect 2 strain bacterium and produce leavening viable count, each production leavening viable count>=10
9individual/mL, treats as fermenting-ripening, if viable count < 10
9individual/mL, continues to cultivate, until reach 10
9individual/mL.
The preparation of 2.3 powder freeze-drying lactobacillus
The production leavening of maturation is aseptically imported in glass ampoule, liquid level 0.8-1cm, after adding a cover bottle stopper, put into-30 DEG C of refrigerator-freezer quick-frozens, by glass ampoule pallet splendid attire after freezing, put into freeze dryer and carry out freeze drying.
2.4 throw type leavens composite
By freeze-dried vaccine powder weight, get A bacterium powder 3-5 part, B bacterium powder 2-3 part, fully make throw type leaven after mixing, for compoiste fermented edible extract of Chinese traditional medicine, the addition of throw type leaven is 0.01% of compound edible extract of Chinese traditional medicine weight.
3. the preparation of composite probiotics ferment plant beverage
3.1 edible Chinese medicine compatibilities
Field thistle 15g, Chinese yam 20g, hawthorn 20g, fructus cannabis 5g, cassia seed 5g, date 15g, fruit of Chinese wolfberry 15g, Poria cocos 10g, honeysuckle 15g, dandelion 20g, spina date seed 5g, root of kudzu vine 10g
3.2 extract preparations
The edible Chinese medicine of 3.1 step compatibilities is added 1L drinking water, and heating boils maintenance 5 minutes, filters, the filtered fluid drinking water of acquisition is settled to 1L and is extract after being cooled to 25 DEG C.
3.3 batching
Add FOS powder by extracting liquid volume, addition is 3.5g/L, stirs, and FOS is fully dissolved.
3.4 high-temperature sterilization
Heating material to the 121 DEG C sterilizing 3.3 steps prepared 20 minutes, is then cooled to 30 DEG C.
3.5 inoculum fermented
0.01% throw type leaven prepared of inoculation 2.4 steps of weight of material is prepared, 30 DEG C of static gas wave refrigerator 36 hours by 3.3 steps.
3.6 sterile filling
The zymotic fluid sterile filling 3.5 steps prepared, is composite probiotics ferment plant beverage.
Embodiment two:
1. the selection of fermentation strain and the preparation of culture medium
Fermentation strain is purchased in Chinese industrial Microbiological Culture Collection administrative center, as follows respectively:
A bacterium---Lactobacillus rhamnosus, Lactobacillus rhamnosus, bacterial strain deposit number is:
B bacterium---lactobacillus paracasei, Lactobacillus paracasei, bacterial strain deposit number is:
A bacterium, B bacterium culture medium are: casein peptone 10.0g, beef extract 10.0g, dusty yeast 5.0g, glucose 5.0g, sodium acetate 5.0g, dibasic ammonium citrate 2.0g, Tween 801.0g, K
2hPO
42.0g, MgSO
4.7H
2o 0.2g, MnSO
4.H
2o 0.05g, CaCO
320.0g, distilled water 1.0L, pH6.8.
2. the preparation process of throw type leaven
The activation of 2.1 freeze-dried vaccine powder
Measure the physiological saline 10mL of 0.9% respectively in 2 in vitro, within 20 minutes, be cooled to 30 DEG C through 121 DEG C of sterilizings, all poured under aseptic conditions in the physiological saline of 0.9% by the freeze-dried vaccine powder in 2 strain bacterium ampullas, concussion makes it dissolve, in 30 DEG C of insulating boxs, static activation 30 minutes, for subsequent use.
2.2 expand cultivation
2.2.1 the preparation of mother culture
Measure each 200mL of 2 strain bacterium culture medium respectively in 2 500mL triangular flasks, 121 DEG C of sterilizings are cooled to 30 DEG C in 20 minutes, by activated bacterial classification in 5% inoculation 2.1 steps of culture volume, 30 DEG C of static gas wave refrigerator 24 hours, as mother culture.
2.2.2 the preparation of leavening is produced
First the defatted milk emulsion that mass percent is 10% is prepared, liquid glycerin, 2% xylo-oligosaccharide, 2% sucrose of 5% is added by defatted milk emulsion weight, 121 DEG C of sterilizings are cooled to 30 DEG C in 20 minutes, press defatted milk emulsion 5% volume ratio inoculation mother culture respectively, then 30 DEG C of static gas wave refrigerator 36 hours, detect 2 strain bacterium and produce leavening viable count, each production leavening viable count>=10
9individual/mL, treats as fermenting-ripening, if viable count < 10
9individual/mL, continues to cultivate, until reach 10
9individual/mL.
The preparation of 2.3 powder freeze-drying lactobacillus
The production leavening of maturation is aseptically imported in glass ampoule, liquid level 0.8-1cm, after adding a cover bottle stopper, put into-30 DEG C of refrigerator-freezer quick-frozens, by glass ampoule pallet splendid attire after freezing, put into freeze dryer and carry out freeze drying.
2.4 throw type leavens composite
By freeze-dried vaccine powder weight, get A bacterium powder 3-5 part, B bacterium powder 2-3 part, fully make throw type leaven after mixing, for compoiste fermented edible extract of Chinese traditional medicine, the addition of throw type leaven is 0.01% of compound edible extract of Chinese traditional medicine weight.
3. the preparation of composite probiotics ferment plant beverage
3.1 edible Chinese medicine compatibilities
Field thistle 20g, Chinese yam 25g, hawthorn 30g, fructus cannabis 10g, cassia seed 10g, date 20g, fruit of Chinese wolfberry 20g, Poria cocos 15g, honeysuckle 20g, dandelion 30g, spina date seed 10g, root of kudzu vine 20g
3.2 extract preparations
The edible Chinese medicine of 3.1 step compatibilities is added 1L drinking water, and heating boils maintenance 5 minutes, filters, the filtered fluid drinking water of acquisition is settled to 1L and is extract after being cooled to 25 DEG C.
3.3 batching
Add FOS powder by extracting liquid volume, addition is 3.5g/L, stirs, and FOS is fully dissolved.
3.4 high-temperature sterilization
Heating material to the 121 DEG C sterilizing 3.3 steps prepared 20 minutes, is then cooled to 30 DEG C.
3.5 inoculum fermented
0.01% throw type leaven prepared of inoculation 2.4 steps of weight of material is prepared, 30 DEG C of static gas wave refrigerator 36 hours by 3.3 steps.
3.6 sterile filling
The zymotic fluid sterile filling 3.5 steps prepared, is composite probiotics ferment plant beverage.
Claims (2)
1. a preparation method for composite probiotics ferment plant beverage, is characterized in that it is prepared according to the following steps:
1). the selection of fermentation strain and the preparation of culture medium
Fermentation strain is purchased in Chinese industrial Microbiological Culture Collection administrative center, as follows respectively:
A bacterium---Lactobacillus rhamnosus, Lactobacillus rhamnosus, bacterial strain deposit number is:
B bacterium---lactobacillus paracasei, Lactobacillus paracasei, bacterial strain deposit number is:
A bacterium, B bacterium culture medium are: casein peptone 10.0g, beef extract 10.0g, dusty yeast 5.0g, glucose 5.0g, sodium acetate 5.0g, dibasic ammonium citrate 2.0g, Tween 80 1.0g, K
2hPO
42.0g, MgSO
4.7H
2o 0.2g, MnSO
4.H
2o 0.05g, CaCO
320.0g, distilled water 1.0L, pH6.8;
2). the preparation process of throw type leaven
2.1) activation of freeze-dried vaccine powder
Measure the physiological saline 10mL of 0.9% respectively in 2 in vitro, within 20 minutes, be cooled to 30 DEG C through 121 DEG C of sterilizings, all poured under aseptic conditions in the physiological saline of 0.9% by the freeze-dried vaccine powder in 2 strain bacterium ampullas, concussion makes it dissolve, in 30 DEG C of insulating boxs, static activation 30 minutes, for subsequent use;
2.2) cultivation is expanded
2.2.1) preparation of mother culture
Measure each 200mL of 2 strain bacterium culture medium respectively in 2 500mL triangular flasks, 121 DEG C of sterilizings are cooled to 30 DEG C in 20 minutes, 5% inoculation 2.1 by culture volume) bacterial classification activated in step, 30 DEG C of static gas wave refrigerator 24 hours, as mother culture;
2.2.2) preparation of leavening is produced
First the defatted milk emulsion that mass percent is 10% is prepared, liquid glycerin, 2% xylo-oligosaccharide, 2% sucrose of 5% is added by defatted milk emulsion weight, 121 DEG C of sterilizings are cooled to 30 DEG C in 20 minutes, press defatted milk emulsion 5% volume ratio inoculation mother culture respectively, then 30 DEG C of static gas wave refrigerator 36 hours, detect 2 strain bacterium and produce leavening viable count, each production leavening viable count>=10
9individual/mL, treats as fermenting-ripening, if viable count < 10
9individual/mL, continues to cultivate, until reach 10
9individual/mL;
2.3) preparation of powder freeze-drying lactobacillus
The production leavening of maturation is aseptically imported in glass ampoule, liquid level 0.8-1cm, after adding a cover bottle stopper, put into-30 DEG C of refrigerator-freezer quick-frozens, by glass ampoule pallet splendid attire after freezing, put into freeze dryer and carry out freeze drying;
2.4) throw type leaven is composite
By freeze-dried vaccine powder weight, get A bacterium powder 3-5 part, B bacterium powder 2-3 part, fully make throw type leaven after mixing, for compoiste fermented edible extract of Chinese traditional medicine, the addition of throw type leaven is 0.01% of compound edible extract of Chinese traditional medicine weight;
3). the preparation of composite probiotics ferment plant beverage
3.1) edible Chinese medicine compatibility
Field thistle 15-20g, Chinese yam 20-25g, hawthorn 20-30g, fructus cannabis 5-10g, cassia seed 5-10g, date 15-20g, fruit of Chinese wolfberry 15-20g, Poria cocos 10-15g, honeysuckle 15-20g, dandelion 20-30g, spina date seed 5-10g, root of kudzu vine 10-20g
3.2) extract preparation
By 3.1) the edible Chinese medicine of step compatibility adds 1L drinking water, and heating boils maintenance 5 minutes, filters, the filtered fluid drinking water of acquisition is settled to 1L and is extract after being cooled to 25 DEG C;
3.3) prepare burden
Add FOS powder by extracting liquid volume, addition is 3.5g/L, stirs, and FOS is fully dissolved;
3.4) high-temperature sterilization
By 3.3) heating material to the 121 DEG C sterilizing for preparing of step 20 minutes, be then cooled to 30 DEG C;
3.5) inoculum fermented
By 3.3) step prepares 0.01% inoculation 2.4 of weight of material) throw type leaven prepared of step, 30 DEG C of static gas wave refrigerator 36 hours.
3.6) sterile filling
By 3.5) zymotic fluid sterile filling prepared by step, be composite probiotics ferment plant beverage.
2. by composite probiotics ferment plant beverage prepared by method according to claim 1.
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Cited By (7)
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| CN105495212A (en) * | 2015-12-17 | 2016-04-20 | 青州伟格生物科技有限公司 | Microorganism and Chinese herb ferment drink and production technology thereof |
| CN105495230A (en) * | 2015-12-01 | 2016-04-20 | 武汉市极活元生物工程有限公司 | Lactobacillus fermented plant drink and preparation method thereof |
| CN106962939A (en) * | 2017-04-11 | 2017-07-21 | 姜红成 | Prepare the fermentation composition and preparation method of the plant enzyme with weight-reducing degreasing effect |
| CN107319230A (en) * | 2017-05-17 | 2017-11-07 | 天津岂均生物科技有限公司 | Fermented beverage with regulation disorders of lipid metabolism function is prepared and applied |
| CN109805234A (en) * | 2019-03-18 | 2019-05-28 | 倪俊刚 | A kind of probiotics fermention plant beverage and preparation method |
| CN114982892A (en) * | 2022-07-04 | 2022-09-02 | 黄河三角洲京博化工研究院有限公司 | Grifola frondosa-pome fruit probiotic compound fermented beverage and preparation method thereof |
| CN117089580A (en) * | 2023-10-19 | 2023-11-21 | 山东向日葵生物工程有限公司 | Lactobacillus casei SF-L-12 fermented product and preparation process and application thereof |
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| CN105495230A (en) * | 2015-12-01 | 2016-04-20 | 武汉市极活元生物工程有限公司 | Lactobacillus fermented plant drink and preparation method thereof |
| CN105495230B (en) * | 2015-12-01 | 2018-08-07 | 武汉市极活元生物工程有限公司 | A kind of lactobacillus-fermented plant beverage and preparation method thereof |
| CN105495212A (en) * | 2015-12-17 | 2016-04-20 | 青州伟格生物科技有限公司 | Microorganism and Chinese herb ferment drink and production technology thereof |
| CN106962939A (en) * | 2017-04-11 | 2017-07-21 | 姜红成 | Prepare the fermentation composition and preparation method of the plant enzyme with weight-reducing degreasing effect |
| CN107319230A (en) * | 2017-05-17 | 2017-11-07 | 天津岂均生物科技有限公司 | Fermented beverage with regulation disorders of lipid metabolism function is prepared and applied |
| CN109805234A (en) * | 2019-03-18 | 2019-05-28 | 倪俊刚 | A kind of probiotics fermention plant beverage and preparation method |
| CN114982892A (en) * | 2022-07-04 | 2022-09-02 | 黄河三角洲京博化工研究院有限公司 | Grifola frondosa-pome fruit probiotic compound fermented beverage and preparation method thereof |
| CN117089580A (en) * | 2023-10-19 | 2023-11-21 | 山东向日葵生物工程有限公司 | Lactobacillus casei SF-L-12 fermented product and preparation process and application thereof |
| CN117089580B (en) * | 2023-10-19 | 2024-02-02 | 山东向日葵生物工程有限公司 | Lactobacillus casei SF-L-12 fermented product and preparation process and application thereof |
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Application publication date: 20150708 |