A kind of tobacco seedling method
Technical field
The present invention relates to botanical seedling culturing field, be specifically related to a kind of tobacco seedling method.
Background technology
Modern installations origin of agriculture, the 1950's, is the product of modern scientific theory and technical development.The basic theories of modern installations agricultural is the plant " theory of mineral nutrition " that 19 th Century German scientists propose.Within 1842, Lee's J.von ratio wishes the report making to be entitled as " Chemical activator is in agricultural and physiology " in AAAS.After this mineral theory of plant nutrition is gradually by people are accepted.About 1851 ~ 1856 years, the J.-B. Bu Senge mineral nutrition of sandy culture research plant, within 1699, R.B. Woodward water culture studied the nutrition of mint.J.von saxophone was in 1860, and W. Ke Nuopu has established the water culture technology in modern age in 1861.The other basic technology of modern installations agricultural is the theory that American scientist F.W.Went in 1949 proposes manual simulation's ecotope.From then on, people are increasingly strong with pursuit for the exploration of modern installations agricultural.
It is self-evident for developing the agriculture importance and necessity for China of efficient modern installations, without superfluous words.But starting late of China's modern installations agricultural, China's most widely used industry of modern installations agricultural is tobacco seedling, flower plants and nursery stock cultivation, growing vegetables, raising rice seedlings, corn nursery etc.Should say the agricultural facility such as booth, greenhouse, plant factor to apply the contribution of the current agriculture stable development of China be vital.
But face the technical bottleneck of some keys in modern installations agricultural utilization process in decades all the time.Such as, the specification of tobacco seedling culture canopy, tobacco seedling factory and corresponding configuration are that in current facility agriculture field, level is the highest, therefore and the tobacco seedling effect come also does not allow tobacco planting family satisfied comprise temperature adjustment, humidity regulation, Illumination adjusting, nutrition adjustment, but.The existing tobacco booth (nursery factory) of China adopts floating seedlings mode, and its management is loaded down with trivial details, operation is many, recruitment is many; Light, temperature, the tobacco growing key element such as wet, fertile affect significantly by various objective variable, and regulation and control difficulty is large; Emerge irregular, need repeatedly thinning to fill the gaps with seedlings; Breeding time, amplitude of fluctuation was large, wayward transplanting time; Hardening complex operation, not easy-regulating, also can damage cigarette seedling sometimes; Especially cigarette seedling quality aspect, tobacco root system is flourishing not, stem is very thin, regularity is not high; Disease hazard rate is higher; After transplanting, also seedling stage is long.
The tobacco academy of sciences of Guizhou Province discloses a kind of flue-cured tobacco three-dimensional aerial fog seedling-cultivating method in patent application CN201310375234.X, and this seedling-cultivating method comprises the following steps: (1) makes cultivating pool; (2) nursery support is made; (3) cultivation plate is made; (4) sow; (5) liquid storage tank is made; (6) nutrient solution is prepared; (7) the atomization circulatory system is set; (8) seedbed management.This seedling-cultivating method temperature and humidity easily controls, and Automated condtrol rate is high, and energy using water wisely, improves rich water utilization ratio, promotes can effectively reduce costs in arid area; Absorption ability of nutrient is strong, and tobacco seedling growth is fast, and quality is good; Do not need light filling measure, comparatively multilayer nursery reduces seedling cost.This three-dimensional aerial fog culture facility is applicable to vegetables, flowers and other crops plantation simultaneously, greatly improves seedling raising greenhouse comprehensive utilization ratio, effectively improves income.
But still only relate in the method and the basic parameters such as the temperature and humidity of nursery are regulated and controled, and the key factor do not related to water, gas, fertilizer, light etc. affect nursery is intervened and regulates and controls.Therefore, this area needs one more fully tobacco seedling method, especially a kind of tobacco seedling autocontrol method.
Summary of the invention
Therefore, the invention provides a kind of tobacco seedling method, the temperature (temperature) in described method, the aerosol nursery of plant related to, wet (humidity), water (spray cycles), gas (oxygen and CO
2), fertile (mineral salt, pH value), light (blade face intensity of illumination) etc. the key factor that affects botanical seedling culturing and growth carries out comprehensive auto-control.
The invention provides a kind of tobacco seedling method, described method comprises the nursery of use aerosol seedling nursing device, and to temperature, humidity, spray cycles, dissolved oxygen amount, CO that described tobacco seedling is cultivated
2, mineral salt, pH value and these factors affecting botanical seedling culturing and growth of blade face intensity of illumination regulate and control comprehensively; Described aerosol seedling nursing device is arranged in booth, described aerosol seedling nursing device comprise rest on the ground liquid storage pool, be positioned at the support member above liquid storage pool, the seedling-cultivating tray be positioned on support member, be positioned at the matrix of seedling-cultivating tray, and described liquid storage pool, support member, seedling-cultivating tray and matrix form a hollow and lighttight aerosol chamber jointly; The nutrient solution for spraying is loaded with in described liquid storage pool.
In the present invention, described booth refers to the agricultural facility such as traditional booth, greenhouse, plant factor's (glass room, factory building), and these facilities are referred to as booth herein, booth has good sealing and heat insulating ability, and the top cover printing opacity of booth.Those skilled in the art's easy understand, the Temperature Humidity Sensor in the present invention can be the Temperature Humidity Sensor be wholely set, and also can be independent temperature sensor and humidity sensor.
In method of the present invention, controller is preferably used to carry out comprehensive auto-control to each factor that tobacco seedling is cultivated.
In a kind of concrete embodiment, in described aerosol chamber, temperature controls at 12 ~ 32 DEG C, and preferably its temperature is 16 ~ 28 DEG C; In described nutrient solution, temperature controls at 12 ~ 35 DEG C, and preferably its temperature is 20 ~ 25 DEG C; In described booth, temperature controls at≤35 DEG C, and preferably its temperature is 25 ~ 35 DEG C.
In a kind of concrete embodiment, in tobacco seedling process, in described aerosol chamber, humid control is 95% ~ 100%; In described booth, humid control is ≤95%, and preferably its humidity is≤90%.Those skilled in the art can understand ground, and described humidity refers to relative moisture.
In a kind of concrete embodiment, in tobacco seedling process, CO in described aerosol chamber
2concentration controls≤0.28%, and preferably its concentration is≤0.03%; CO in described booth
2concentration controls>=0.03%.
In a kind of concrete embodiment, in tobacco seedling process, the blade face intensity of illumination of tobacco seedling controls in >=2000 luxs.
In a kind of concrete embodiment, in tobacco seedling process, the spray cycles controlled in aerosol chamber makes described soil humidity control as 90-100%; Control nutrient solution EC and be 2-4ms/cm; Control nutrient solution pH value and be 5.5-7, be preferably 5.5-6.5; The dissolved oxygen concentration controlled in nutrient solution is 5-8ppm.
In a kind of concrete embodiment, in described nutrient solution, be provided with liquid level gauge, dissolved oxygen sensor, pH meter, EC meter and temperature sensor.
Preferably, atomizer, the CO for nutrient solution being become aerosol is contained in described aerosol chamber
2analyzer and Temperature Humidity Sensor, described nutrient solution forms aerosol be passed to atomizer under the effect of pump after, and is provided with the heater for heating nutrient solution at nutrient solution from liquid storage pool to the pipeline of atomizer; Containing for the blower fan making gas exchanges in aerosol chamber on the support member of described aerosol chamber.
Preferably, containing nutrition stock solution container, fresh water container, pump, water nozzle, light supplementing lamp for plants, Temperature Humidity Sensor, CO in the booth outside described aerosol chamber
2analyzer, intensity of illumination meter; Described nutrition stock solution container and fresh water container add nutrient solution by measuring pump and pipeline in liquid storage pool, and clear water sprays downwards from the water nozzle be arranged in above seedling-cultivating tray from fresh water container under the effect of pipeline and pump, described light supplementing lamp for plants and described intensity of illumination meter are all positioned at above seedling-cultivating tray.
In a kind of concrete embodiment, described support member comprises base and support, and described nutrition stock solution container comprises A liquid container, B liquid container and C liquid container.Described A liquid container 3, B liquid container 4 and C liquid container 5 such as can be respectively for splendid attire acid solution and alkali lye (for regulating the pH value of nutrient solution) and the container for splendid attire inorganic salt solution.
In a kind of concrete embodiment, described nutrient solution is passed to atomizer after device after filtration under the effect of pump, and described filter comprises the first filter core and the second filter core from outside to inside, and described filter is arranged in the groove of liquid storage pool.
In a kind of concrete embodiment, controller is used to connect the measuring pump of described nutrition stock solution reservoir, measuring pump, liquid level gauge, dissolved oxygen sensor, pH meter, EC meter, temperature sensor, Temperature Humidity Sensor, heater, pump, blower fan, light supplementing lamp for plants, the CO of fresh water container connection
2analyzer and intensity of illumination meter.
Preferably, to the pipeline of atomizer, be also provided with hydrostate from liquid storage pool at nutrient solution, and described hydrostate is connected with controller.
In a kind of concrete embodiment, inside described booth roof or arranged outside have sunshade net, containing for the blower fan of gas exchanges inside and outside booth on described booth, and described blower fan is connected with controller.
In a kind of concrete embodiment, described controller is also connected with monitor terminal.
In a kind of concrete embodiment, also in described matrix, be provided with Temperature Humidity Sensor, and this Temperature Humidity Sensor is connected with controller.
In a kind of concrete embodiment, be also outside equipped with Temperature Humidity Sensor at described booth, and this Temperature Humidity Sensor is connected with controller.
In a kind of concrete embodiment, described digitalization precision seedling system is the seedling system for tobacco.
Operation principle of the present invention is: (1) distinguishes each growth and development environment factor level of perception by inductor, and the information digitalization of perception; (2) information transmission is to calculator; (3) calculator processes data, and makes decisions; (4) calculator implements running command to terminal device, realizes carrying out regulating and control to the corresponding growth and development environment factor.
The farming decision system of this invention can take agriculture specialist system as foundation, then in conjunction with the factor such as varieties of plant, planting season, planting environment, plantation target call of user, for user provides optimum simulation and control module; User can according to actual needs and objective condition select the simulation that is suitable for and control module voluntarily.This invention can realize people and surmount the hope that natural season carries out agricultural planting, can plant at any time; Plant is in superior growth and development environment all the time, thus shortens the crop production cycle, significantly improves crop yield, improves crop quality; Water saving, joint are fertile, and not applying pesticides substantially; Significantly can reduce the Cultivate administration cost of booth.
Information digitalization system of the present invention comprises information acquisition module, information transmission modular, data processing module.The maincenter of data processing module is growth and development of plants environment data base, and calculator collects the digitized information of Real-time Collection and immediately carries out analyzing and processing, forms terminal mechanism operating instruction.The content of following five bulks is at least comprised: nutrition mechanism, light governor motion, thermoregulation mechanism, humidity regulation mechanism, O in digitalization precision seedling system provided by the invention
2/ CO
2governor motion.
Accompanying drawing explanation
A kind of schematic diagram of digitalization precision seedling system of Fig. 1 for providing in the present invention.
Wherein, 1, controller, 2, monitor terminal, 3, A liquid container, 4, B liquid container, 5, C liquid container, 6, fresh water container, 7, measuring pump, 8, nutrient solution, 9, liquid level gauge, 10, dissolved oxygen sensor, 11, pH meter, 12, EC counts, 13, temperature sensor, 14, Temperature Humidity Sensor, 15, first filter core, 16, second filter core, 17, heater, 18, pump, 19, hydrostate, 201, atomizer, 202, water nozzle, 21, blower fan, 22, light supplementing lamp for plants, 23, liquid storage pool, 24, base, 25, support, 26, seedling-cultivating tray, 27, matrix, 28, seedling, 29, booth, 30, CO
2analyzer, 31, intensity of illumination meter, 32, sunshade net.
Embodiment
In the present invention, following embodiment is used for going on to say the present invention, but the scope that following embodiment is not intended to limit the present invention.
Embodiment 1
As shown in Figure 1, the digitalization precision seedling system related in tobacco seedling method of the present invention relates to following structure:
One, booth: newly-built or utilize existing plastic tunnel or greenhouse, require booth or greenhouse transparent, seal, have can switch ventilating opening, fly net, sunshade net are installed.The specification in booth or greenhouse can be built according to existence conditions or demand.Described booth or greenhouse mainly provide a kind of daylighting, insulation, the space environment of the relative closure of insect protected, digitalization precision aerosol seedling system can carry out suitable coupling according to the real space of booth.
Two, mechanism is cultivated: cultivation mechanism comprises cultivation support, liquid storage pool, aerosol chamber, seedling-cultivating tray.The dark 30cm of liquid storage pool, the wide 110cm in pond, bottom surface is the gradient of 5 ‰, flat smooth, leakproof, spreads thickness 0.1mm polyethylene blown film (replacing concrete to play waterproof action) at the bottom of pond.Cultivation is placed on liquid storage pool, and its base length and width are identical with liquid storage pool, and its top structure is such as circular arc for top cross-sectional, and summit is to base bottom surface height 35-50cm.Aerosol chamber is the confined space formed after being combined by liquid storage pool, cultivation support and seedling-cultivating tray, is wherein provided with nutrient liquid atomizing nozzle.Seedling-cultivating tray is placed in the top of support, for loading matrix, supports seed and seedling.Distance in booth between every two cultivation supports keeps 35-50cm, so that operation.
Cultivation support provides direct physical support for seed and seedling.The nutrient solution that liquid storage pool falls after rise after collecting atomization.Aerosol chamber provides a necessary dark space for root growth, and has the effect of heat and moisture preserving, is also the functional space of nutrient liquid atomizing.
Three, nutrition mechanism: comprise the A liquid storage bin (storage inorganic salt liquid) that nutrient solution is provided, B liquid storage bin (storage acid solution), C liquid storage bin (storage alkali lye), clear water cassette for supplying, flow pump, liquid level gauge, liquid storage pool, feed pump, water inlet pipe, water outlet main pipe, main, arm, shower nozzle, nozzle, nutrient solution recovery tube, filter, pressure regulator valve, manometer, flowmeter, relay, water pipe inspection switch, temperature sensor, liquid level sensor, flow sensor, pressure sensor, EC sensor, pH sensor, dissolved oxygen sensor.
Nutritional principles: spray to after nutrient liquid atomizing bottom seedling-cultivating tray, nutrient solution droplet enters in dish cave, by matrix absorption, for seed and seedling provide moisture, nutrition and heat before root system stretches out seedling-cultivating tray bottom aperture by seedling-cultivating tray bottom aperture; After root system stretches out seedling-cultivating tray bottom aperture, root system directly can adhere to the little droplet of nutrient solution of atomization and absorb its moisture provided and nutrient.Nutrient solution pumps up by water pump from liquid supply box, sprays to bottom seedling-cultivating tray through parts such as filter, flowmeter, pressure regulator valves from nozzle by nutrient solution with fine mist; Unabsorbed nutrient solution falls back to the liquid storage pool bottom aerosol chamber.The water level of liquid storage pool, by the monitoring of liquid level sensor, stores the monitoring of nutrient solution by temperature sensor, EC sensor, pH sensor, dissolved oxygen sensor of liquid case, and water outlet main pipe pressure is stressed sensor, flow sensor monitoring.Nutrient liquid atomizing drop diameter is 30-60um.The area of pump power, operating pressure and actual load and nozzle matches.The Liquid level compensator of liquid storage pool is by the supply of liquid level sensor, EC sensor, pH sensor, flow sensor co-controlling A liquid storage bin, B liquid storage bin, C liquid storage bin, clear water cassette for supplying.
Four: thermoregulation mechanism and operation principle
Thermoregulation mechanism: comprise heater (the timely heater of frequency conversion type), temperature sensor, sunshade net, blower fan, aerial spraying device.
Operation principle: the temperature controlled macroscopical threshold value in aerosol chamber is 12-32 DEG C, and the detector of temperature sensor is arranged in aerosol chamber, for calculator provides real time temperature administrative decision parameter.Heater 17 is arranged on feed flow supervisor, heats the nutrient solution being about to atomization ejection.When needs heat up, after starting spraying water pump, instant heater starts to heat nutrient solution, and heat takes in aerosol chamber and matrix by the nutrient solution of atomization, to maintain the preference temperature needed for seed germination and growth of seedling.When needs reduce temperature, in conjunction with functional entities such as the corresponding startup sunshade net of relevant parameter, water nozzle 202, blower fans.
Five, humidity regulation mechanism and operation principle
Humidity regulation mechanism: its functional entity comprises blower fan 21 and water nozzle 202.When booth humidity too high and its higher than outside humidity time, by booth inner air and outer air exchange to reduce air humidity in booth; When booth humidity is too low, water nozzle 202 starts spraying to improve air humidity in booth.
Six, Illumination adjusting mechanism and operation principle
Illumination adjusting mechanism, its functional entity is sunshade net and LED light supplement lamp.The covered with sunshade net when intensity of illumination exceedes Plant Light saturation point or indoor temperature is too high; When intensity of illumination is lower than Plant Light compensation point, starts LED light supplement lamp and carry out light filling.
Seven, CO
2/o
2concentration adjustment mechanism and operation principle
CO
2/o
2concentration adjustment mechanism: its functional entity is mini fan and the booth blower fan of aerosol chamber.The mini fan timing of aerosol chamber is aerosol chamber's air-supply, to discharge CO wherein
2, simultaneously supplement O
2; To regulate the CO in booth during the fan operation of booth
2/o
2concentration.
Eight, intelligent digitalized control software design:
Described intelligent digitalized control software design comprises the Controlling model for different plant variety.According to the difference of kind, productive target, the production schedule, this software has laid in a large amount of construction modules and Controlling model, and user can a direct key preference pattern, also can according to respective demand construction module assembled model.
In sum, in the digitalization precision seedling system related in method provided by the invention, the control methods of each parameter are as follows:
One, temperature
1, aerosol chamber's temperature: have temperature sensor to detect its temperature in aerosol chamber; When the temperature is too low, mainly through heater 17 ducted nutrient solution heated aerosol indoor temperature is raised; When aerosol indoor temperature is too high, mainly through the lower clear water of spray in water nozzle 201, aerosol indoor temperature is reduced.
2, greenhouse temperature: have temperature sensor to detect its temperature in booth; When the temperature is too low, adopt booth to carry thermal source to heat up; When temperature is too high, is ventilated by the blower fan 21 be positioned on booth and open sunshade net cooling.
Two, humidity
1, aerosol chamber's humidity: regulate aerosol chamber's humidity by atomizer 201 and the blower fan 21 be positioned on support member.
2, booth humidity: regulate booth humidity by dehumidifier, blower fan 21, atomizer 201 and water nozzle 202.
Three, CO
2
1, aerosol chamber CO
2: the CO ventilating to make aerosol chamber by the blower fan 21 be positioned on support member
2value maintains lower than certain data point.
2, booth CO
2: to be ventilated by the blower fan 21 be positioned on booth and by using CO
2make CO in booth
2value maintains higher than certain data point.
Four, illumination
Blade face illumination: provide illumination to make the illumination value on blade face maintain higher than certain data point by the light supplementing lamp for plants 22 be positioned at above blade face; When natural lighting is crossed strong, enable sunshade net.
Five, nutrition
1, soil humidity: mainly through controlling the flow of atomizer 201 to make soil humidity higher than certain data point.
2, liquid storage pool liquid level: mainly through controlling first from fresh water container 6 to injected clear water liquid storage pool, then make nutrition pit level be not less than certain value from the mode that nutrition stock solution container injects nutrition stock solution to liquid storage pool.
3, nutrient solution EC: nutrient solution EC value is controlled a suitable scope by the ratio controlled between the clear water amount of injection liquid storage pool and nutrition stock solution amount.
4, solution temperature: control its temperature mainly through adjustment nutrition stock solution container, fresh water container 6 to the aperture of the injection rate in liquid storage pool 23 and heater 17, also can have other heating and cooling mode.
5, nutrient solution pH value: introduce acid respectively or alkali regulates nutrient solution pH value from two different nutrition stock solution containers.
6, nutrient solution dissolved oxygen amount: mainly through being positioned at blower fan 21 on support member to regulate the permeability of aerosol chamber, the dissolved oxygen amount of nutrient solution is maintained in certain scope.
In a kind of concrete embodiment, the described corresponding state modulator of digitalization precision seedling system for tobacco is as shown in table 1.
Table 1
Embodiment 2
The using method of the digitalization precision seedling system related in tobacco seedling method provided by the invention is as follows:
Build, install, debug Hao Ge mechanism, device.
Get out matrix, seed; According to formulated nutrient solution, acid solution, alkali lye.Matrix, seed, nutrient solution are according to seed and cultivate target decision.
Get out seedling-cultivating tray, according to the kind of seedling and the seedling-cultivating tray of cultivation target selection dimension.
Nutrient solution is filled it up with in nutrition pond, and each liquid reserve tank fills nutrient solution mother liquor, and acid solution, alkali lye are respectively charged into acid solution bucket, lye tank.
Matrix sterilization, seedling-cultivating tray sterilization cleaning, cultivating stand sterilization cleaning.
Matrix is loaded in seedling-cultivating tray.Notice how much load the matrix of seedling-cultivating tray will be suitable for, can not too consolidation, both can not the remarkable sedimentation because of water suction when will ensure that matrix is in working order simultaneously, guarantee matrix can form complete capillary network again.
Type of seeding: the sowing cave extruding bore 10mm, dark 0.4-0.6mm after seedling-cultivating tray filling matrix with pressure cave device in matrix, is sown into sowing cave central authorities, on seedling-cultivating tray, then evenly sows one deck matrix to cover seed by seed.
After planting make matrix contain water saturation in the mode of spraying to matrix water spray (clean river, well water, pond on a hill water), then seedling-cultivating tray is placed on cultivation support.
Select suitable control module, set special parameter.
Open each functional entity and controlling organization, culture facility enters Automated condtrol running status.
Receive each nutrient solution mother liquor, acid solution, the alarm of alkali lye deficiency or other abnormal conditions alarm time, carry out manual intervention or disposal.
Artificial thinning, leaf-cutting; The human assistance of correlation function mechanism.
Embodiment 3
The result of use of the digitalization precision seedling system related in the inventive method:
Comparative trial is carried out in digitalization precision seedling system provided by the invention and conventional seedbed system mode (water planting) nursery, the two tobacco seed adopting leaf tobacco production producing region identical, sows on the same day and runs.The nursery that table 2 gives the two contrasts situation, and the seedling quality that table 3 gives the two contrasts situation.
Table 2
Table 3
|
Plant height cm |
Root heavy (g/ strain) |
The long cm of root |
The thick mm of stem |
The present invention |
6 |
0.875 |
75 |
6 |
Conventional seedbed system |
4.8 |
0.65 |
35 |
4.5 |
1, the tobacco seedling adopting the technology of the present invention to cultivate, field planting shows as and significantly improves tobacco leaf per unit area yield, hierarchical organization, per mu yield value.
2, accurately control nursery stage: high efficiency regulatory light, temperature, water, gas, fertilizer, effectively control cigarette seedling and become seedling stage, 60 days seedlings.And this system can also be shifted to an earlier date or the delay seedling time by technical regulation appropriateness according to physioclimate and production actual needs.Precisely implementing of transplanting time arrangement can be realized, guarantee that every relevant farming arrangement meets physioclimate rule to greatest extent, fully effectively utilize physioclimate resource.
3, seedling is healthy and strong: seedlings root is flourishing, stem is sturdy, straight uniform; Land for growing field crops also seedling is fast, within 0-5 days, goes back seedling; Fast growth after the cigarette transplantation of seedlings of high-quality to land for growing field crops, growth is neat, is convenient to field management, is conducive to the raising of yield of tobacco quality and the increase of benefit.
4, save space: the present invention adopts three-dimensional layout, effectively improve booth space availability ratio more than 20%, significantly improve nursery utilization of area rate.
5, efficiently reduce costs: intelligentized control method is carried out to Nutrition Management, Temperature and Humidity Control, hardening, illumination etc., do not need manual operation, and have more more scientific than labor management, more precisely, more system, more stable excellent management effect, the efficiency of management is high, the recruitment that significantly can reduce seedling management drops into, and reduces management cost.This product nursery stage only about 60, faster than existing seedling raising manners 20 ~ 30 days, highly shortened seedling raise period, significantly reduces seedling cost.
6, environmental protection: seedling raising environment is clean, substantially without agricultural chemicals, eliminates pesticide pollution, saves extermination of disease and insect pest cost.
7, complete intelligent digitalized control, easy and simple to handle, is easy to promote.
In sum, use the inventive method can shorten growing-seedling period, cultivate more healthy and strong seedling, save seedling culture space, efficiently reduce costs and environmental protection.
The foregoing is only the preferred embodiments of the present invention, be not limited to the present invention, for a person skilled in the art, the present invention can have various modifications and variations.Within the spirit and principles in the present invention all, any amendment done, equivalent replacement, improvement etc., all should be included within protection scope of the present invention.