CN104672343A - Extracting and purifying method of raffino-oligosaccharide - Google Patents
Extracting and purifying method of raffino-oligosaccharide Download PDFInfo
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- CN104672343A CN104672343A CN201510071504.7A CN201510071504A CN104672343A CN 104672343 A CN104672343 A CN 104672343A CN 201510071504 A CN201510071504 A CN 201510071504A CN 104672343 A CN104672343 A CN 104672343A
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Abstract
The invention discloses an extracting and purifying method of raffino-oligosaccharide. The extracting and purifying method comprises the following steps: removing greases from cotton seeds, collecting cotton slag, crushing, dissolving with water, adding alkali, extracting by microwave, centrifugally separating, filtering, collecting filtrate, adding a flocculating agent, adjusting pH (potential of hydrogen) value, standing, centrifugally separating, filtering, collecting filtrate, de-coloring by active carbon, filtering, collecting filtrate, carrying out ultra-filtration treatment on filtrate, collecting a penetrating solution, carrying out nano-filtration treatment on the penetrating solution, collecting a penetrating solution, concentrating, and drying, so as to obtain the raffino-oligosaccharide. By adopting proper film cut-off molecular weight and film components and effective film cleaning method in an ultra-filtration system and nano-filtration system, the running stability of equipment is guaranteed; by utilizing the centrifugal separation technology, a filter assistant is not required, the operation is convenient, the process is simple, the equipment investment and running cost are low, and the extracting efficiency of the raffino-oligosaccharide reaches 75%.
Description
Technical field
The present invention relates to technical field of natural product extraction, especially a kind of Isolation and purification method of cottonseed oligose.
Background technology
Oligose refers to the compound be combined into glycosidic link by 2 ~ 10 monose, and it is not easily digested.Oligose can improve human body micro-ecological environment, be conducive to the propagation of bifidus bacillus and other probiotics, producing organic acid through metabolism makes intestinal pH reduce, suppress the growth of Salmonellas and spoilage organism in intestines, regulate gastrointestinal function, suppress corrupt substance in intestines, change stool, prevent treating constipation, and increase VITAMIN synthesis, improve immune function of human body; In addition, oligose can hypoglycemic, reducing blood-fat.Therefore, oligose is widely used in, in milk-product, lactobacillus drink, bifidobacterium yoghurt, cereal foods and protective foods, being especially applied in the food of infant and the elderly as a kind of food ingredients.
Oligose is mainly present in soybean, cottonseed, eucalyptus, beet, agueweed root; oligose in cottonseed and raffinose; raffinose can bacterial equilibrium in regulating intestinal canal; recover intestines wall original state and form protective layer; protection intestinal health; prevent a large amount of generations of objectionable impurities in enteron aisle, alleviate burden of liver, thus play the effect preventing and treat various hepatopathy diseases.
The extracting method of oligose generally adopts flooding, alkali lye lixiviate, membrane separation technique etc., and wherein, water extract method efficiency is lower, though alkali lye lixiviate efficiency is higher, but the operating time is long; And existing membrane separation process often complex process.
Summary of the invention
The object of this invention is to provide a kind of Isolation and purification method of cottonseed oligose, the Isolation and purification method of this cottonseed oligose can solve Isolation and purification time length, inefficient problem.
In order to solve the problem, the technical solution used in the present invention is:
The Isolation and purification method of this cottonseed oligose comprises the following steps:
A, adopt first squeeze leaches afterwards method removing cottonseed in grease, collection squeeze after cottonseed disintegrating slag, pulverizing;
B, to be dissolved in water, to add alkaline solution, proceed in Microwave Extraction Apparatus and extract 15 minutes ~ 25 minutes;
C, proceed to centrifugation in whizzer, filter, collect filtrate;
D, add flocculation agent, regulator solution pH value, leave standstill, centrifugal, filter, collect filtrate;
E, employing gac carry out desolventing technology, filter, collect filtrate;
F, filtrate described in E step is proceeded to ultrafiltration system carry out uf processing, collect permeate;
G, E step gained permeate is proceeded to nanofiltration system carry out nanofiltration process, collect permeate
H, concentrated, dry, obtain cottonseed oligosaccharide.
In technique scheme, scheme can also be more specifically: in step C, centrifugal rotational speed is 3000 revs/min ~ 3800 revs/min, and in D step, centrifugal rotational speed is 3000 revs/min ~ 4000 revs/min.
Further, in D step, pH value is adjusted to 4.2 ~ 5.
Further, the pressure in F step during uf processing is 0.03 MPa ~ 0.45 MPa, and temperature is 25 DEG C ~ 40 DEG C.
Further, the pressure in G step during nanofiltration process is 0.8 MPa ~ 1.2 MPa, and temperature is 30 DEG C ~ 40 DEG C.
Further, flocculation agent is chitosan.
Owing to have employed technique scheme, the present invention compared with prior art has following beneficial effect:
1, ultrafiltration system of the present invention and nanofiltration system adopt the retaining molecular weight and membrane module and effective Membrane cleaning method that are suitable for, ensure the stability that equipment runs; Adopt repeatedly centrifugation technique, without the need to adding flocculating aids, easy and simple to handle.
2, present invention process is simple, facility investment and working cost low, the extraction efficiency of cottonseed oligose reaches 75%.
Embodiment
Below in conjunction with embodiment, the invention will be further described:
Embodiment 1
The Isolation and purification method of the present embodiment cottonseed oligose is: adopt the grease in the method removing cottonseed first squeezing and leach afterwards, collect the cottonseed disintegrating slag after squeezing, pulverize, be dissolved in water, add sodium hydroxide solution, proceed in Microwave Extraction Apparatus and extract 15 minutes, proceed in whizzer and carry out centrifugation, centrifugal rotational speed is regulated to be 3800 revs/min, filter, collect filtrate, add chitosan solution, regulator solution pH value to 4.2, leave standstill, centrifugal, centrifugal rotational speed is regulated to be 3500 revs/min, filter, collect filtrate, gac is adopted to carry out desolventing technology, filter, collect filtrate, filtrate is proceeded to ultrafiltration system and carry out uf processing, pressure when arranging uf processing is 0.45 MPa, temperature is 25 DEG C, collect permeate, permeate is proceeded to nanofiltration system and carry out nanofiltration process, pressure when arranging nanofiltration process is 1.2 MPas, temperature is 30 DEG C, collect permeate, concentrated, dry, obtain cottonseed oligosaccharide.
Embodiment 2
The Isolation and purification method of the present embodiment cottonseed oligose is: adopt the grease in the method removing cottonseed first squeezing and leach afterwards, collect the cottonseed disintegrating slag after squeezing, pulverize, be dissolved in water, add sodium hydroxide solution, proceed in Microwave Extraction Apparatus and extract 20 minutes, proceed in whizzer and carry out centrifugation, centrifugal rotational speed is regulated to be 3400 revs/min, filter, collect filtrate, add chitosan solution, regulator solution pH value to 5, leave standstill, centrifugal, centrifugal rotational speed is regulated to be 3000 revs/min, filter, collect filtrate, gac is adopted to carry out desolventing technology, filter, collect filtrate, filtrate is proceeded to ultrafiltration system and carry out uf processing, pressure when arranging uf processing is 0.25 MPa, temperature is 32 DEG C, collect permeate, permeate is proceeded to nanofiltration system and carry out nanofiltration process, pressure when arranging nanofiltration process is 1 MPa, temperature is 35 DEG C, collect permeate, concentrated, dry, obtain cottonseed oligosaccharide.
Embodiment 3
The Isolation and purification method of the present embodiment cottonseed oligose is: adopt the grease in the method removing cottonseed first squeezing and leach afterwards, collect the cottonseed disintegrating slag after squeezing, pulverize, be dissolved in water, add sodium hydroxide solution, proceed in Microwave Extraction Apparatus and extract 25 minutes, proceed in whizzer and carry out centrifugation, centrifugal rotational speed is regulated to be 3000 revs/min, filter, collect filtrate, add chitosan solution, regulator solution pH value to 4.6, leave standstill, centrifugal, centrifugal rotational speed is regulated to be 4000 revs/min, filter, collect filtrate, gac is adopted to carry out desolventing technology, filter, collect filtrate, filtrate is proceeded to ultrafiltration system and carry out uf processing, pressure when arranging uf processing is 0.03 MPa, temperature is 40 DEG C, collect permeate, permeate is proceeded to nanofiltration system and carry out nanofiltration process, pressure when arranging nanofiltration process is 1.2 MPas, temperature is 40 DEG C, collect permeate, concentrated, dry, obtain cottonseed oligosaccharide.
Claims (6)
1. an Isolation and purification method for cottonseed oligose, is characterized in that comprising the following steps:
A, adopt first squeeze leaches afterwards method removing cottonseed in grease, collection squeeze after cottonseed disintegrating slag, pulverizing;
B, to be dissolved in water, to add alkaline solution, proceed in Microwave Extraction Apparatus and extract 15 minutes ~ 25 minutes;
C, proceed to centrifugation in whizzer, filter, collect filtrate;
D, add flocculation agent, regulator solution pH value, leave standstill, centrifugal, filter, collect filtrate;
E, employing gac carry out desolventing technology, filter, collect filtrate;
F, filtrate described in E step is proceeded to ultrafiltration system carry out uf processing, collect permeate;
G, E step gained permeate is proceeded to nanofiltration system carry out nanofiltration process, collect permeate
H, concentrated, dry, obtain cottonseed oligosaccharide.
2. the Isolation and purification method of cottonseed oligose according to claim 1, is characterized in that: in step C, centrifugal rotational speed is 3000 revs/min ~ 3800 revs/min, and in D step, centrifugal rotational speed is 3000 revs/min ~ 4000 revs/min.
3. the Isolation and purification method of cottonseed oligose according to claim 1, is characterized in that: in D step, pH value is adjusted to 4.2 ~ 5.
4. the Isolation and purification method of cottonseed oligose according to claim 1, is characterized in that: the pressure in F step during uf processing is 0.03 MPa ~ 0.45 MPa, and temperature is 25 DEG C ~ 40 DEG C.
5. the Isolation and purification method of cottonseed oligose according to claim 1, is characterized in that: the pressure in G step during nanofiltration process is 0.8 MPa ~ 1.2 MPa, and temperature is 30 DEG C ~ 40 DEG C.
6. the Isolation and purification method of cottonseed oligose according to claim 1, is characterized in that: described flocculation agent is chitosan.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201510071504.7A CN104672343A (en) | 2015-02-11 | 2015-02-11 | Extracting and purifying method of raffino-oligosaccharide |
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| CN201510071504.7A CN104672343A (en) | 2015-02-11 | 2015-02-11 | Extracting and purifying method of raffino-oligosaccharide |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105815414A (en) * | 2016-06-07 | 2016-08-03 | 河南科技大学 | Quinoa cookie and preparation method for same |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102351917A (en) * | 2011-09-14 | 2012-02-15 | 北京科技大学 | Method for extracting raffinose from cotton seed meal |
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- 2015-02-11 CN CN201510071504.7A patent/CN104672343A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102351917A (en) * | 2011-09-14 | 2012-02-15 | 北京科技大学 | Method for extracting raffinose from cotton seed meal |
Non-Patent Citations (5)
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| 刘峥 等: "微波法提取大豆中低聚糖的研究", 《食品研究与开发》 * |
| 吴尧 等: "微波辅助提取棉籽糖及膜纯化工艺研究", 《湖北工业大学学报》 * |
| 宋春丽 等: "超声波和微波辅助提取大豆低聚糖的工艺比较", 《食品与机械》 * |
| 宿曼 等: "超滤法分离棉籽粕水提液中的棉籽低聚糖", 《食品与发酵工业》 * |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105815414A (en) * | 2016-06-07 | 2016-08-03 | 河南科技大学 | Quinoa cookie and preparation method for same |
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Application publication date: 20150603 |