CN104488718A - Quick propagation method for oreocharis flavida - Google Patents
Quick propagation method for oreocharis flavida Download PDFInfo
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- CN104488718A CN104488718A CN201410811927.3A CN201410811927A CN104488718A CN 104488718 A CN104488718 A CN 104488718A CN 201410811927 A CN201410811927 A CN 201410811927A CN 104488718 A CN104488718 A CN 104488718A
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Abstract
The invention discloses a quick propagation method for oreocharis flavida. The method comprises the following steps: selection and sterilization of an explant; primary culture; multiplication of subculture; rooting culture; and inoculation. By taking tender leaves as the explant, media for the primary culture, the subculture and the rooting culture are set, and the oreocharis flavida is quickly propagated under the condition that the temperature is 24-28 DEG C, the lighting time is 9h/d and the illumination intensity is 1500lx. The method disclosed by the invention is simple and practical to operate, the rooting rate can reach 100%, and plants are robust.
Description
Technical field
The invention belongs to technical field of plant propagation, be specifically related to the method for quickly breeding of a kind of chrysanthemum horse bell lettuce tongue.
Background technology
Gesneriaceae is ornamental plants very prevailing in the world in recent years, and about there is 150 genus 3700 kinds in the whole world, and China is distributed with 58 genus more than 470 and plants, and this University of Science and Technology Partial Species has higher ornamental value, but mostly still quiet among deep and secluded valley, remote mountains.The western countries such as the U.S., Canada have been bred as a large amount of garden-varieties.Hainan Gesneriaceae has endemic genus 2, endemic species 10, and most kind plant types is compact, and the various floridity of color, the florescence is long, is the valuable source of development of new flower variety.Chrysanthemum horse bell lettuce tongue (Oreocharis flavida Merr.) belongs to herbaceos perennial for Gesneriaceae horse bell lettuce tongue, is the distinctive Wild Flowers in Hainan.Phyllopodium is raw, spends faint yellow, is applicable to potted plant viewing and admiring, directly can tames application.Root-like stock is thick and short.The whole base of leaf is raw, tool long handle; Blade is avette to width egg shape, and rare wide ellipse is to obovate, long 4 ~ 10 centimetres, wide 2 ~ 6.5 centimetres, top is circular, and base portion is near heart-shaped or circular, the nearly full edge in edge or have shallow cognate, above close by pubescence, close by yellowish-brown thin,tough silk shape wool below, 5 ~ 7, the every limit of lateral vein, below obviously; Long 2.5 ~ 10 centimetres of petiole, close by yellowish-brown thin,tough silk shape wool.The tissue-culturing rapid propagation of chrysanthemum horse bell lettuce tongue can provide seedling for its artificial cultivation, has important realistic meaning to its application and protection of resources.The application carries out tissue culture technical research to chrysanthemum horse bell lettuce tongue first, and for applying of Hainan Conandron ramondioides sieb. Et zuce flowers provides technical support, achievement in research will be filled up domestic and study blank inside the province.
Summary of the invention
The object of the present invention is to provide the method for quickly breeding of a kind of chrysanthemum horse bell lettuce tongue, by utilizing chrysanthemum horse bell lettuce tongue blade as explant, and utilizing aseptic vanelets to reach the object of Fast-propagation seedling.
The present invention realizes especially by following technical scheme:
A method for quickly breeding for chrysanthemum horse bell lettuce tongue, comprises the following steps: the 1) selection of explant and sterilizing; 2) just in generation (adventitious bud inducing), cultivates; 3) shoot proliferation is cultivated; 4) culture of rootage; 5) inoculate.
Explant described in step (1) selects young leaflet tablet.Described sterilizing detailed process is: 70% alcohol-pickled 10s, aseptic water washing, uses 0.02%HgCl
2solution disinfection 3min, aseptic water washing, to sterilize 15min with 2%NaClO, aseptic water washing is clean.
Culture medium prescription in step (2): the grand 6ml/L+ sucrose 30g/L of MS+6-BA 0.5mg/L+NAA 0.1mg/L+ benefit training, pH value 6.5.
Culture medium prescription in step (3): adventitious bud proliferation bud: MS+6-BA 0.1mg/L+NAA0.1mg/L+ sucrose 30g/L, pH value 6.5; Aseptic plant vanelets propagation bud: MS+6-BA0.5mg/L+NAA 0.1mg/L+ sucrose 30g/L, pH value 6.5;
Culture medium prescription in step (4): MS+IBA 0.5mg/L+ sucrose 30g/L, pH value 6.5.
Step of the present invention (2) ~ (4) condition of culture is: cultivation temperature 24 ~ 28 DEG C, light application time 9h/d, intensity of illumination 1500lx.
Beneficial effect of the present invention is: the present invention utilizes chrysanthemum horse bell lettuce tongue blade as explant, and in inducing culture, the training of interpolation benefit is grand achieves good effect, rooting rate 100%, and average every strain takes root 6.00, robust plant, and leaf look green.
Embodiment
Below in conjunction with embodiment, the present invention is described further, the following stated, only to preferred embodiment of the present invention, not do other forms of restriction to the present invention, any those skilled in the art may utilize the technology contents of above-mentioned announcement to be changed to the Equivalent embodiments of equal change.Everyly do not depart from the present invention program's content, any simple modification done following examples according to technical spirit of the present invention or equivalent variations, all drop in protection scope of the present invention.
Embodiment 1
This experimental establishment is carried out in Zu Pei factory of landscape flower research institute of Academy of Agricultural Sciences of Hainan Province, and the enforcement time is year September in May, 2013 to 2014.Each set handling all inoculates 8 bags, every bag of 3 materials, repeats for 3 times, all medium all additional carragheen 9g/L, sucrose 30g/L.
1, materials and methods
1.1 explant
Young leaflet tablet.
1.2 method
1.2.1 the acquisition of sterilizable material
Cut the young leaflet tablet of band petiole from plant, with cotton ball libation at an ancient wedding ceremony cleanser essence wiping blade two sides, then clean up with running water.With 70% alcohol-pickled 10s on superclean bench, aseptic water washing 1 time, then use 0.02%HgCl
2solution disinfection 3min, aseptic water washing 5 times, finally to sterilize 15min with 2%NaClO, aseptic water washing 5 times.Blade is cut into the square fritter of 1 ㎝ and is inoculated in upward in Initial culture base with blade face.
1.2.2 just in generation (adventitious bud inducing), cultivates
1.2.2.1 culture medium prescription
The grand 6ml/L+ sucrose 30g/L of MS+6-BA 0.5mg/L+NAA 0.1mg/L+ benefit training, pH value 6.5.
1.2.2.2 condition of culture
Cultivation temperature 24 ~ 28 DEG C, light application time 9h/d, intensity of illumination 1500lx.
1.2.2.3 inoculation
After explant inoculation 15d, blade face starts to expand protuberance, and 20d starts to occur callus, and 40d all differentiates indefinite bud at blade edge, main lobe arteries and veins base portion and blade face place.
1.2.3 shoot proliferation is cultivated
1.2.3.1 culture medium prescription
Adventitious bud proliferation bud: MS+6-BA 0.1mg/L+NAA 0.1mg/L+ sucrose 30g/L, pH value 6.5.
Aseptic plant vanelets propagation bud: MS+6-BA 0.5mg/L+NAA 0.1mg/L+ sucrose 30g/L, pH value 6.5.
1.2.3.2 condition of culture
Cultivation temperature 24 ~ 28 DEG C, light application time 9h/d, intensity of illumination 1500lx.。
1.2.3.3 inoculation
The indefinite bud clump induced is cut into simple bud, is seeded on proliferated culture medium and cultivates, 40d growth coefficient is 8.19.
In aseptic vanelets access medium, 20d starts to occur callus, and 40d starts differentiation and sprouts, and 50d indefinite bud reaches higher peak, 50d adventitious bud induction frequency 100%, average every leaf indefinite bud number 32.33.
1.2.4 culture of rootage
1.2.4.1 culture medium prescription
MS+IBA0.5mg/L+ sucrose 30g/L, pH value 6.5.
1.2.4.2 condition of culture
Cultivation temperature 24 ~ 28 DEG C, light application time 9h/d, intensity of illumination 1500lx.。
1.2.4.3 inoculation
Cut in the indefinite bud access root media of high about 1.5cm, about 15d starts to take root, well developed root system after 30d, rooting rate 100%, and average every strain takes root 7.51, robust plant, and leaf look green.
Claims (7)
1. a method for quickly breeding for chrysanthemum horse bell lettuce tongue, is characterized in that comprising the following steps: the 1) selection of explant and sterilizing; 2) Initial culture; 3) shoot proliferation is cultivated; 4) culture of rootage; 5) inoculate.
2. the method for quickly breeding of a kind of chrysanthemum horse bell lettuce tongue according to claim 1, is characterized in that: step (1) sterilization process is: 70% alcohol-pickled 10s, aseptic water washing, uses 0.02%HgCl
2solution disinfection 3min, aseptic water washing, to sterilize 15min with 2%NaClO, aseptic water washing is clean.
3. the method for quickly breeding of a kind of chrysanthemum horse bell lettuce tongue according to claim 1, is characterized in that: in step (2), culture medium prescription is: the grand 6ml/L+ sucrose 30g/L of MS+6-BA 0.5mg/L+NAA0.1mg/L+ benefit training, pH value 6.5.
4. the method for quickly breeding of a kind of chrysanthemum horse bell lettuce tongue according to claim 1, is characterized in that: in step (3), culture medium prescription is: adventitious bud proliferation bud: MS+6-BA0.1mg/L+NAA 0.1mg/L+ sucrose 30g/L, pH value 6.5; Aseptic plant vanelets propagation bud: MS+6-BA 0.5mg/L+NAA 0.1mg/L+ sucrose 30g/L, pH value 6.5.
5. the method for quickly breeding of a kind of chrysanthemum horse bell lettuce tongue according to claim 1, is characterized in that: in step (4), culture medium prescription is: MS+IBA 0.5mg/L+ sucrose 30g/L, pH value 6.5.
6. the method for quickly breeding of a kind of chrysanthemum horse bell lettuce tongue according to any one of claim 3 ~ 5, is characterized in that: condition of culture is temperature 24 ~ 28 DEG C, light application time 9h/d, intensity of illumination 1500lx.
7. the method for quickly breeding of a kind of chrysanthemum horse bell lettuce tongue according to claim 1, is characterized in that: described explant selects young leaflet tablet.
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| CN201410811927.3A CN104488718B (en) | 2014-12-23 | 2014-12-23 | A kind of rapid propagation method of chrysanthemum horse bell lettuce tongue |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN105519447A (en) * | 2016-02-29 | 2016-04-27 | 广西壮族自治区药用植物园 | Lagarosolen jingxiensis tissue culture leaf two-step seedling growing method |
| CN113367064A (en) * | 2021-07-01 | 2021-09-10 | 上海为绿景观建设有限公司 | Method for cultivating lantern trees |
| CN115633637A (en) * | 2022-09-21 | 2023-01-24 | 广西壮族自治区中国科学院广西植物研究所 | Tissue culture method of chirita cordifolia |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105519447A (en) * | 2016-02-29 | 2016-04-27 | 广西壮族自治区药用植物园 | Lagarosolen jingxiensis tissue culture leaf two-step seedling growing method |
| CN113367064A (en) * | 2021-07-01 | 2021-09-10 | 上海为绿景观建设有限公司 | Method for cultivating lantern trees |
| CN115633637A (en) * | 2022-09-21 | 2023-01-24 | 广西壮族自治区中国科学院广西植物研究所 | Tissue culture method of chirita cordifolia |
| CN115633637B (en) * | 2022-09-21 | 2023-11-07 | 广西壮族自治区中国科学院广西植物研究所 | Tissue culture method of herba corianthi radicis |
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Address after: 571100 Hainan Province, Haikou City Qiongshan District Xindan Road No. 14 Applicant after: TROPICAL HORTICULTURE RESEARCH INSTITUTE OF HAINAN ACADEMY OF AGRICULTURAL SCIENCE Address before: 571100 Hainan Province, Haikou City Qiongshan District Xindan Road No. 14 Applicant before: Institute of Garden Flowers, Hainan Academy of Agricultural Sciences |
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