CN104341538B - A kind of method for separating and preparing of high HG content Helianthi pectin - Google Patents
A kind of method for separating and preparing of high HG content Helianthi pectin Download PDFInfo
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- CN104341538B CN104341538B CN201310332506.8A CN201310332506A CN104341538B CN 104341538 B CN104341538 B CN 104341538B CN 201310332506 A CN201310332506 A CN 201310332506A CN 104341538 B CN104341538 B CN 104341538B
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Abstract
The invention discloses the extracting method of the Helianthi pectin of a kind of high HG content, implementation step is as follows: the sunflower plate tap water after drying and crushing is rinsed well, ethanol boils pretreatment, 0.2% oxalic acid solution boils and carries, filtrate carries out alcohol analysis after concentrating, gained precipitation is the sunflower plate pectin rich in HG.The present invention has simple to operate, and pectin productivity is high, and galacturonic acid content is high, the advantages such as pectin color is pure.
Description
Technical field
The invention belongs to life science, be specifically related to a kind of method extracting HG type pectin from sunflower plate.
Background technology
Pectin is the class vegetable polysaccharides rich in galacturonic acid (GalA), different according to the methyl-esterification degree of GalA, can be by
Pectin is divided into high-esterpectin and low methoxyl pectin.Esterification degree >=50% of high-esterpectin, mainly for the production of Food & Drink etc..
High-esterpectin Gelation Conditions is wayward, has certain requirement to pH, solid content etc..The esterification degree of low methoxyl pectin
＜ 50%, gets final product plastic under conditions of low-concentration metallic ion exists, more easy to control than the Gelation Conditions of high-esterpectin, therefore
Apply the most extensive.Pectin has excellent gelling, thickening, outstanding solidifying and Stabilization, be widely applied to milk product, fruit juice,
The food production industries such as fruit jam, fruit jelly, confection and the non-food industries such as pharmacy, daily use chemicals field, development prospect is wide.American-European,
The developed area in the world such as Japan, Hong-Kong, pectin is that in food service industry and people's life, indispensable food adds
Agent.Pectin is at starting stage in the production of China with application as a kind of food additive.Though some producer proceeds by fruit
The development and production of glue, but quality and quantitative aspects are the most undesirable, still have part to need import.The total output of domestic pectin is still with height
Ester pectin is main, constitutes about the 75% of total amount.Low methoxyl pectin is mainly obtained after chemical treatment by high-esterpectin.Utilize China
Substantial amounts of food industry regenerated resources (such as peel of Citrus reticulata Blanco, pomace, sunflower plate and bar etc.) carry out the high-quality pectin of large-scale production,
Significant for development China's food and pharmaceutical industries.Along with food with low sugar content increasingly gets consumer reception, low methoxyl pectin
Yield and demand all can have greatly increased.Therefore, the low methoxyl pectin in sunflower plate, meet very much the current market demand.
Helianthi is the industrial crops that China is important, has substantial amounts of sunflower plate and bar to be discarded as agricultural residue every year.
Low methoxyl pectin is extracted, it is possible to making full use of the agricultural by-products such as sunflower plate and bar, that increases farmers' income is same from sunflower plate
Time also make resource be fully used, meet country recycling economy is advocated and requirement.
The present invention, in order to simplify extraction process step, improves yield and product quality, devises that a set of operating process is simple, pectin
Quality better, the sunflower plate pectin extraction method of yield high energy-saving environment protection.
Summary of the invention
The present invention uses the present invention to pass through alcohol pre-treatment, and high-temperature acidic solution extracts, ethanol precipitation, vacuum dehydrating at lower temperature obtain
High yield high-quality sunflower plate low methoxyl pectin, implements step as follows:
(1) sunflower plate after drying and crushing is carried out alcohol pre-treatment, sunflower plate is boiled 15 minutes with 85% ethanol
To 30 minutes, after filtering ethanol, with distilled water immersion 3 to 6 hours；
(2) filter, obtain pectin filtrate, add 0.2% oxalic acid solution, pH2～3, temperature according to the ratio of solid-liquid ratio 1:8
Degree is 90～100 DEG C, extraction time 1～2 hours, extracts twice；
(3) liquid, 60 DEG C of concentrations are merged；
(4) in fructose concentrating liquid, add ethanol solution, make ethanol final concentration of 60%, stand 4～8 hours, centrifugal
Precipitate to pectin；
(5) carry out cold drying or spray drying after pectin precipitation water dissolution, obtain the sunflower plate pectin rich in HG.
Sunflower plate pectin productivity prepared by the present invention is 20%, and galacturonic acid content is higher than 80%, and esterification degree is less than 25%,
Acid insoluble ash is less than 1%, has product colour pure, and yield is high, and ash is few, galacturonic acid content advantages of higher.Before
The ethanol processed and consume during alcohol analysis can be with recycling, it is possible to effectively reduces waste material and discharges, low-carbon environment-friendly；Produce
Sunflower plate residue may be used for producing feedstuff；It is multiple that the low methoxyl pectin obtained can apply to food, pharmaceutical raw material and adjuvant etc.
Field.
Detailed description of the invention:
The enforcement step of the present embodiment is as follows:
Taking dry sunflower plate 500 grams, pulverize, tap water rinses, and adds 85% ethanol of 2.5 liters, heated and boiled 15 points
Clock.Cool to room temperature, filter, residue 4 liters of distilled water immersions 3 hours.The grass of 0.2% is added in the ratio of solid-liquid ratio 1:8
Acid solution, boils and carries 2 hours, and after filtration, residue continues to boil and carries 1 hour.Merge the filtrate obtained for twice, be concentrated to 1.5 liters,
4000 rpms, centrifugal 10 minutes, add 95% ethanol extremely final concentration of 60%, stand 4～12 hours, 4000 turns
Per minute, centrifugal 10 minutes, with carrying out cold drying after water dissolution, obtain the sunflower plate pectin rich in HG.Pectin is received
Rate is 22%, galacturonic acid content 81%, and esterification degree is 19%, acid insoluble ash 0.37%, and index of correlation all meets state
Family's standard-required to food additive pectin.
Claims (1)
1. the preparation method of the sunflower plate pectin of one kind high HG content, it is characterised in that concrete operation step is as follows:
(1) sunflower plate after drying and crushing is carried out alcohol pre-treatment, sunflower plate is boiled 15 minutes with 85% ethanol
To 30 minutes, after filtering ethanol, with distilled water immersion 3 to 6 hours；
(2) filter, obtain pectin filtrate, add 0.2% oxalic acid solution, pH2～3, temperature according to the ratio of solid-liquid ratio 1:8
Degree is 90～100 DEG C, extraction time 1～2 hours, extracts twice；
(3) liquid, 60 DEG C of concentrations are merged；
(4) in fructose concentrating liquid, add ethanol solution, make ethanol final concentration of 60%, stand 4～8 hours, centrifugal
Precipitate to pectin；
(5) carry out cold drying or spray drying after pectin precipitation water dissolution, obtain the sunflower plate pectin rich in HG.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310332506.8A CN104341538B (en) | 2013-08-01 | 2013-08-01 | A kind of method for separating and preparing of high HG content Helianthi pectin |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310332506.8A CN104341538B (en) | 2013-08-01 | 2013-08-01 | A kind of method for separating and preparing of high HG content Helianthi pectin |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN104341538A CN104341538A (en) | 2015-02-11 |
| CN104341538B true CN104341538B (en) | 2016-10-26 |
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| CN201310332506.8A Active CN104341538B (en) | 2013-08-01 | 2013-08-01 | A kind of method for separating and preparing of high HG content Helianthi pectin |
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Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106519068B (en) * | 2016-12-27 | 2019-06-11 | 晨光生物科技集团股份有限公司 | A kind of industrialized preparing process of sunflower plate low-ester pectin |
| CN107474157A (en) * | 2017-08-28 | 2017-12-15 | 广西钟山县富强雅瑶华香农业有限公司 | A kind of extracting method of sunflower pectin |
| CN109553699B (en) * | 2019-01-07 | 2023-10-03 | 中北大学 | Method for extracting low-ester pectin from fresh sunflower trays and stems by using acid |
| CN109762076B (en) * | 2019-01-28 | 2020-04-17 | 江南大学 | Method for simultaneously extracting pectin and protein from sunflower discs |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1079871A (en) * | 1993-06-10 | 1993-12-29 | 张文沂 | The extracting method of low ester pectin from sunflower |
| CN1490339A (en) * | 2002-10-19 | 2004-04-21 | 王丽华 | Pectin preparation by biochemical method |
| CN101160327A (en) * | 2005-03-09 | 2008-04-09 | 卡吉尔股份有限公司 | Process for obtaining pectin |
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2013
- 2013-08-01 CN CN201310332506.8A patent/CN104341538B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1079871A (en) * | 1993-06-10 | 1993-12-29 | 张文沂 | The extracting method of low ester pectin from sunflower |
| CN1490339A (en) * | 2002-10-19 | 2004-04-21 | 王丽华 | Pectin preparation by biochemical method |
| CN101160327A (en) * | 2005-03-09 | 2008-04-09 | 卡吉尔股份有限公司 | Process for obtaining pectin |
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| CN104341538A (en) | 2015-02-11 |
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