CN104303735A - GAP (good agricultural practices) cultivation method for leaf rosemary yard - Google Patents
GAP (good agricultural practices) cultivation method for leaf rosemary yard Download PDFInfo
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- CN104303735A CN104303735A CN201410432944.6A CN201410432944A CN104303735A CN 104303735 A CN104303735 A CN 104303735A CN 201410432944 A CN201410432944 A CN 201410432944A CN 104303735 A CN104303735 A CN 104303735A
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Abstract
The invention discloses a GAP (good agricultural practices) cultivation method for a leaf rosemary yard. The GAP cultivation method comprises the following steps: establishing and managing the leaf rosemary yard, cultivating leaf rosemary seedlings, determining a planting period, performing planting management, determining planting density, fertilizing, performing field management, performing pest and disease control, and determining a rosemary leaf harvesting period and a rosemary leaf harvesting method. By the GAP cultivation method, the problem of standardization of herb production can be effectively solved; the GAP cultivation method plays an extremely important role in ensuring the safety, high efficiency, stability and controllable quality of the leaf rosemary; the planting method and the product quality meet requirements of national normative documents; the yield and the quality can be effectively improved; the Promote standardized and industrial development of leaf rosemary planting can be promoted; the GAP cultivation method is conducive to environment protection and ecological balance; water and soil loss can be prevented; sustainable utilization of rosemary leaf resources can be achieved; through standardized, intensive and industrial rosemary planting, good economical benefits and ecological benefits can be achieved and industrial upgrade of the rosemary can be promoted.
Description
Method field
The present invention relates to a kind of cultivation method, be specifically related to a kind of leaf rosemary, Xue MingRosma rinus officinalis garden GAP cultivation method.
Background method
Rosemary, Xue MingRosma rinus officinalis (Rosmarinus officinalis Linn.) is dicotyledon (Dicotyledoneae) Labiatae (Labiatae) white dead nettle subfamily (Lamioideae) rosemary, Xue MingRosma rinus officinalis race (Rosmarineae) Rosmarinus (Rosmarinus) plant.English name: Rosemary.Perennial evergreen undershrub, the epitheton of vegetable formal name is developed by two Latins " ros " and " marinus ", mean " dew in sea ", in Europe, rosemary, Xue MingRosma rinus officinalis is by the surrounding of extensively planting in church, the offerings that it hallows by religionist, therefore rosemary, Xue MingRosma rinus officinalis is otherwise known as: " Santa Maria rose ".In recent years due to the discovery to its excellent antioxygenic property, there is implant mass on the ground such as the U.S., India at present.China was introduced a fine variety into from Spain in 2006 by the Chinese Academy of Sciences, and many places also have scale to plant.
Europe has the history in over thousands of year as edible blending seasoning matter application, and its peace property has obtained global extensive accreditation.But the application as antioxidant is also the history of nearly ten years.Because its antioxygenic property is high, safety is reliable, Heat stability is good, and production cost is low, safety non-pollution, has the trend progressively replacing Prof. Du Yucang antioxidant at present.Unique fragrance can be distributed, and there is higher ornamental value, become the afforestation cultivated plant that Mediterranean country is common, wherein have some or soil and slope protection, the desirable kind prevented erosion.At present, the states such as France, Portugal, Spain, the U.S. have grasped the methods such as rosemary, Xue MingRosma rinus officinalis is potted plant, afforestation, field planting, tissue-culturing rapid propagation, cuttage and seedling culture more maturely, and extensive use aborning.In recent years, Japan also started in the greening in Deluxe villa district, adopt this kind, achieved good effect.
In food additives field, the market scale of antioxidant is only second to preservative, is second largest market.The U.S. and Japan are about 3.5 ten thousand tons for the antioxidant of food every year, and present rosemary antioxidant annual production is in the world about 3000 tons, less than 10% of the total market size, due to each side, rosemary antioxidant only can be satisfied with a small amount of high value added product and use.Current imported from America product is about per kilogram 3500-3800 yuan in the price of China, and the market conditions of TBHQ are only per kilogram about 200 yuans, the shortage of the price difference that two kinds of products are huge and domestic rosemary antioxidant production causes present domestic rare producer to adopt the main cause of rosemary antioxidant.
Chemosynthesis antioxidant as food additives, be the world 20th century and before general choice.Because chemosynthesis antioxidant all has larger toxicity, side effect to organs such as human liver, spleen, lungs; at mid-twentieth century; once impacted larger poisoning, some mechanisms such as the World Health Organization (FAO/WHO), European Community's protection of the child tissue (HACSG), biological industry association of Britain (BIBRA) and tissue conduct extensive research the safety issue of chemosynthesis antioxidant.Research shows, chemosynthesis antioxidant all has larger toxicity, side effect to organs such as human liver, spleen, lungs.The McDonald's " chicken McNugget event " continuing up to 2010 is exactly the continuity using chemosynthesis antioxidant to cause food-safety problem.But due to the needs of worldwide marketplace books circulation, people can not find for the moment does not have the antioxidant of toxic and side effect to replace them, for this reason, various countries' associated mechanisms has carried out strict, careful toxicologic study and evaluation to existing antioxidant, formulate detailed use standard, reduce the toxic and side effect of chemosynthesis antioxidant to people.But countries in the world and associated mechanisms, for the concern to human health, all wish to find a kind ofly do not have the natural of toxic and side effect to guarantee food security to the mankind.The natural plant composition extracted from plant, because it is safe, nontoxic or substantially nontoxic, receives the extensive welcome of people, becomes the focus of research and development.Since 20th century, abroad in succession research and develop and extracted the harmful natural of human non-toxic from tealeaves, scurvy grass, tomato, grape pip, Radix Glycyrrhizae, flue-cured tobacco and rosemary, Xue MingRosma rinus officinalis etc. plant.This discovery also causes current Northern European countries to prohibit the use chemosynthesis antioxidant, developed country--European Union, the U.S., Japan etc. strictly restriction use chemosynthesis antioxidant human body being had to toxicity, side effect, and seek and encourage to promote natural, also limit simultaneously or prohibitted the use the food import of chemosynthesis antioxidant.Research finds, in numerous naturals, rosemary natural anti-oxidant, not only there is good non-oxidizability, and good health-care effect is also had to human body, more seldom only have rosemary natural anti-oxidant to have efficiently at present, stablize, resistant to elevated temperatures feature, this discovery, has promoted the research and development of countries in the world to rosemary natural anti-oxidant.Rosemary antioxidant becomes the target that World Developed Countries is competitively developed.In this context, party and country's leader from the strategy of food security, by the Chinese Academy of Sciences in later 1980s, from the U.S. introduce and at national experimental cultivation rosemary, Xue MingRosma rinus officinalis.Result of the test our province Central Yunnan is best suited for one of area of planting, and the content of essential oil and antioxidant is higher than original producton location.Within 1997, rosemary antioxidant is listed in China's antioxidant amendments, within 2009, is formally classified as antioxidant [(04.017), the scope of application: vegetable fat, animal fat, prefabricated, halogen, smoked, burn, roasting, fried meat, Western-style ham, fried food etc.Research on maximum utilized quantity (g/Kg): 0.3-0.7].
Rosemary, Xue MingRosma rinus officinalis is environmentally friendly crop: plant once pipe 10 years, avoid mountain area and repeat ploughing and weeding every year, be conducive to water conservation; Rosemary, Xue MingRosma rinus officinalis plantation survives and does not substantially need pillar afterwards, only needs picture to gather in leek to gather in about 2 times every year, and cost is very low; Rosemary, Xue MingRosma rinus officinalis does not have damage by disease and insect, does not therefore need to use agricultural chemicals; Rosemary, Xue MingRosma rinus officinalis is insensitive to chemical fertilizer, likes bio-fertilizer and farmyard manure, is conducive to improveing soil; Rosemary, Xue MingRosma rinus officinalis in mountain area, upper level plantation, 0.07 hectare produces the fresh branches and leaves of rosemary, Xue MingRosma rinus officinalis per year generally at 2 ~ 3 tons, according to current market price one ton of fresh branches and leaves of rosemary, Xue MingRosma rinus officinalis at about 7000 yuan, that is to say that peasant household is at mountain planting one mu of rosemary, Xue MingRosma rinus officinalis, gross earnings are at about 6000 ~ 8000 yuan.
According to current Chinese human resources market and our production method, produce the high-effect antioxidant of rosemary, Xue MingRosma rinus officinalis, cost is between 200-250 yuan, and common performance antioxidant cost is about 120 yuan, there is the price prerequisite of alternative TBHQ completely, easily for terminal client accepts.In order to improve Competitive Products, considering Product Safety and Development Trends, believing that many businessmans can consider to adopt.The annual turnover of domestic current TBHQ, at 3 ten thousand-5 ten thousand tons, if successfully substitute the market share of 1%, in 400,000 yuan per ton, can take in 1.2-2.0 hundred million yuans by enforcement sale in domestic 1 year.Consider again overseas market, can the occupation rate of market of sustainable development and the first market opportunities, market capacity is very considerable.The investigation of domestic market finds, the vegetable oil sales volume of 1 year is between 6,000 ten thousand ~ 8,000 ten thousand tons, the brand of vegetable oil is as " Jin Longyu ", " Shandong flower ", " good fortune near the house " etc., the antioxidant that the overwhelming majority adopts is TBHQ, yet just like " Jin Longyu " portioned product, also at employing BHA, then can first be substituted completely, and the domestic Vegetable oil market share of " Jin Longyu " accounts for 30%.Domestic and international food antioxidant market situation, the sustainable development for rosemary, Xue MingRosma rinus officinalis product provides very good opportunity to develop.Rosemary, Xue MingRosma rinus officinalis is drought-enduring, barren-resistant, but requires soil not ponding, and comparatively wide to soil pH value accommodation, pH can grow in the soil of 4.5 ~ 8.7.What suitable drain was good is rich in chiltern or loose lime soil.There is certain Salt And Alkali Tolerance ability.Property happiness temperature climate, growth optimum temperature be 9 ~ 30 DEG C.A few days ago, sporadicly rosemary, Xue MingRosma rinus officinalis is planted, as Guizhou, Guangxi, Yunnan etc. in the multiple province of China.Through our research study, Guizhou has been formed commercially produces on a small scale, but industrialization level is not high; Guangxi rosemary, Xue MingRosma rinus officinalis plantation unsatisfactory, will Large Scale Death after growth a period of time, be difficult to the plantation of formation scale; Yunnan rosemary, Xue MingRosma rinus officinalis content is lower, extracts yield not high; Its reason of above problem has to be analyzed.Existing several company is engaged in the production of rosemary, Xue MingRosma rinus officinalis goods.Product has the goods supply domestic and international markets such as Rosemary Oil, fat-soluble carnosic acid, carnosol, water-soluble Rosmarinic acid.
Rosmarinus officinalis extract has efficient, nontoxic antioxidant effect, can be widely used in food, functional food, spices and flavouring and the industry such as perfume, daily-use chemical industry.Rosemary, Xue MingRosma rinus officinalis or universally acknowledged third generation pollution-free food antioxidant (natural antiseptic agent) are the functional food base-materials of great exploitation potential for its, wide market.Have the fragrance product of the title of " soft gold " in the international market, its primary raw material source is exactly these herb plants.Rosemary, Xue MingRosma rinus officinalis is again natural bactericide, has extremely strong killing action to the pathogenic microorganisms such as bacterium, virus, is widely used in urban landscaping in American-European, Japanese and even Taiwan.As long as in highway both sides, park, residential quarters, family's garden implant appropriate rosemary, Xue MingRosma rinus officinalis, it just can give out fragrance constantly all the year, improves ambient air environment, invigorating.Due to rosemary, Xue MingRosma rinus officinalis of many uses, and in biology extraction, also only have certain research and industry development at home, otherwise function function need exploitation, has the great market space and potentiality to be exploited.In food additives field, the market scale of antioxidant is only second to preservative, is second largest market.The U.S. and Japan are about 3.5 ten thousand tons for the antioxidant of food every year, and present rosemary antioxidant annual production is in the world about 3000 tons, less than 10% of the total market size, due to each side, rosemary antioxidant only can be satisfied with a small amount of high value added product and use.Current imported from America product is about per kilogram 3500 ~ 3800 yuans in the price of China, and the market conditions of TBHQ are only per kilogram about 200 yuans, the shortage of the price difference that two kinds of products are huge and domestic rosemary antioxidant production causes present domestic rare producer to adopt the main cause of rosemary antioxidant.According to current Chinese human resources market and our production method, produce the high-effect antioxidant of rosemary, Xue MingRosma rinus officinalis, cost is between 200 ~ 250 yuan, and common performance antioxidant cost is about 120 yuan, there is the price prerequisite of alternative TBHQ completely, easily for terminal client accepts.In order to improve Competitive Products, considering Product Safety and Development Trends, believing that many businessmans can consider to adopt.The annual turnover of domestic current TBHQ, at 30,000 ~ 50,000 tons, if successfully substitute the market share of 1%, in 400,000 yuan per ton, can take in 1.2 ~ 2.0 hundred million yuans by enforcement sale in domestic 1 year.Consider again overseas market, can the occupation rate of market of sustainable development and the first market opportunities, market capacity is very considerable.The investigation of domestic market finds, the vegetable oil sales volume of 1 year is between 6,000 ten thousand ~ 8,000 ten thousand tons, the brand of vegetable oil is as " Jin Longyu ", " Shandong flower ", " good fortune near the house " etc., the antioxidant that the overwhelming majority adopts is TBHQ, yet just like " Jin Longyu " portioned product, also at employing BHA, then can first be substituted completely, and the domestic Vegetable oil market share of " Jin Longyu " accounts for 30%.Domestic and international food antioxidant market situation, the sustainable development for rosemary, Xue MingRosma rinus officinalis product provides very good opportunity to develop.
Summary of the invention
The object of the invention is the GAP cultivation method in order to provide a kind of leaf rosemary, Xue MingRosma rinus officinalis garden, can effectively improve rosemary, Xue MingRosma rinus officinalis seed output and quality, promote leaf rosemary, Xue MingRosma rinus officinalis plantation standardization and industrialization.
The method scheme that the present invention adopts is, the GAP cultivation method in leaf rosemary, Xue MingRosma rinus officinalis garden, comprise the Construction and management in leaf rosemary, Xue MingRosma rinus officinalis garden and leaf rosemary, Xue MingRosma rinus officinalis seedling fostering, planting period, plantation management, plantation density, fertilising, field management, the extermination of disease and insect pest, rosemary leave picking time and collecting method:
One, the leaf construction in rosemary, Xue MingRosma rinus officinalis garden
1, selection of land: select physical features higher, not accumulated water field, mountain soil, dry land;
2, wholely: all turned over by planting site, smash, ploughing depth is 30 centimeters, and the soil ridging turned over has been dug drain ditch; Ridge bar soil face width degree keeps 30 ~ 50 centimeters, the height at the bottom of native face to drain ditch 30 centimeters;
3, land improvement: every mu of farmyard manure 30 ~ 40kg executing to become thoroughly decomposed, phosphate fertilizer 300kg; Then plough deeply, harrow broken, leveling; Bed soil mixes sand;
Two, propagation method
1. seminal propagation: (1) collects seed: rosemary, Xue MingRosma rinus officinalis seed propagation, and in two Room ovarys, only a room is solid, and ripening rate is 11.1%; It is June that seed starts maturation time; After seed collection, 0 DEG C ~ 10 DEG C dry Tibetan in refrigerator; (2) sow: 9 ~ October of sowing time; Smooth furrow face before sowing, after planting, burns soil by dusting cover screen cover 1 layer; Then whole furrow face is covered, timely moisturizing with plastic film shed; (3) seed and seedling management: 1. adopt Seedling bag nursery; Sow 15 ~ 25 days seed sproutings, germination temperature is between 20 ~ 24 DEG C; 2. can be transplanted on Seedling bag when seedling grows to 4 ~ 5cm height, in Seedling bag, 40 ~ 60 days time, keep ground moistening; 3. to apply fertilizer in the second half section in little seedling stage; Clear water drip washing nursery stock blade to be used immediately in order to avoid produce fertilizer damage after each fertilising; 4. before transplanting, seedling at least will carry out the hardening of lO days, transplants seedlings before morning 10:00 and after afternoon 4:00; Transplant nursery stock after 5 ~ 7 days to start to send out roots; 5. transplant after 10 days and carry out the 1st fertilising, execute the aqueous solution that 5g/L composite fertilizer is made into better, executed 1 time every 7 ~ 10 days; Can transplant to land for growing field crops when height of seedling reaches 10 ~ 15cm;
2. cottage propagation: the construction of (1) cutting orchard: will select to garden that fertile soil, viscosity are little, good permeability, physical features is mild, and illumination is sufficient, and draining is good, has abundant water resources, has a good transport service, and is convenient to the place that manages; (2) cuttings is selected and process: 1. cuttage branch selects raw, healthy and strong, maternal plant then without the short semi-lignified branch of damage by disease and insect, pitch, length 8 ~ 12cm; 2. longer or stout and strong branch is cut into several sections, and each section has the joint in more than 4 roads; 1 ~ 2, leaf is stayed in upper end, and stay full leaf, all the other are erased, and lower end is cut into horse ear shape; 3. the cuttings got ready puts into the container that clear water is housed, and uses potassium permanganate sterilization, then use root-inducing powder immersion branch growth-promoting root after soaking 5 ~ 10min; (3) seedbed prepares: 1. leeward facing south should be selected in seedbed, and physical features is smooth, near water source, good permeability, and the sand not easily hardened after watering, high bed is taked in seedbed; 2. within first 1 day, with the liquor potassic permanganate of 1g/L, seedbed is carried out disinfection in cuttage, prepare the front seedbed of cuttage and will to drench water; (4) cutting medium: rosemary, Xue MingRosma rinus officinalis cutting medium selects one of following each mixture: the mixture of the mixture of the mixture of the mixture of peat soil and perlitic mixture, plant ash and river sand soil, sandy loam, perlite and river sand soil, peat soil and loess, peat soil and river sand soil; (5) cutting time and method: 1. the rosemary, Xue MingRosma rinus officinalis cuttage and seedling culture time is 3 ~ April, cutting time at the cloudy day or should carry out afternoon; 2. during cuttage, cuttings is inserted the degree of depth 3 ~ 4cm in soil, insert 2 joints in soil, seeding row spacing 5cm × 5cm; 3. before cuttage, seedbed water is watered; Will water permeable after insertion in time, first time waters and adopts the method for spray, finds that seedling wants timely righting, solid steady, the moisturizing of upper cover plastic film; (6) cuttage seeding management: 1. cuttage seeding is in two weeks, must water every day 1 time, watering time is selected early or evening; Sunlight is strong, temperature height time to shade, irrigation times also will increase; 2., after two weeks, cuttage seeding starts to take root; After cuttings cuttage every 7 days spray 1 time 1500 times chlorothalonil or thunder many Mils bactericide, all to add the potassium dihydrogen phosphate of 0.2% in two kinds of bactericide, cuttings resistance can be strengthened; 3. can open film gradually after cuttage lO days, after 20 ~ 25 days, film all be opened; 4. rosemary, Xue MingRosma rinus officinalis cuttage and seedling culture stays the garden time to need 90 days; Treat that seedling growth is healthy and strong, well developed root system, can transplant when height of seedling reaches more than 10cm; 5. getting the front seedbed of seedling waters permeable, gets Miao Shiying and has digged with a hoe, and allows soil adhere to root system, and is bundled into wisp with straw, to transplant;
3. tissue culture propagation: (1) explant kind and process: 1. the rosemary, Xue MingRosma rinus officinalis explant of tissue cultures comprises blade, stem apex, the stem section on annual spray, its processing method of different explants is different;
(2) blade: 1. detergent liquid first soaks 1h, 75% alcohol-pickled 30s after running water 1h; 2. 17min is soaked, aseptic water washing 3 ~ 4 times with saturated calcium hypochlorite solution again;
(3) stem apex: 1. get greenhouse pot culture rosemary, Xue MingRosma rinus officinalis terminal bud, be placed in running water and rinse 30min, then use the alcohol-pickled 10s of 75%, after aseptic water washing 3 times, add 0.1g/L HgCl
2solution soaks 5min, and often liter of solution adds tween 2; 2. use aseptic water washing 5 ~ 6 times, flushing process will constantly stir; 3. the material of sterilizing is put into sterile beaker when aseptic;
(4) stem section: the branch choosing robust growth, removes blade, drops in washing powder solution and cleans 2 times, and aseptic water washing for several times, then soaks in the clever solution of the many shades of 20ml/L;
(5) sterile working on workbench: the 1. HgCl of the 1st sterilization 1g/L
2solution leaching 3min, with aseptic washing 2 times; 2. the 2nd sterilization is carried out after, with the HgCl of 1g/L
2solution leaching 2min, then with aseptic washing 5 times; 3. the material disinfected is cut into little length of tape 2 ~ 3 bud that 2.0 ~ 3.Ocm is long;
(6) medium is selected: medium is 1/2MS+ sucrose 1.5%+IBAl.0mg/L+NAAO.5mg/L+PP333 0.05mg/L;
(7) plantlet in vitro management: 1. transplant and select in spring; 2. the test-tube plantlet of 3 ~ 4 leaves there is is to move to hardening canopy 7 ~ 10 days long for root 5 ~ 6cm; 3. being poured on by bottle seedling fills in the large basin of running water, washes away basifixed medium gently, notes not damaging root system and cauline leaf; 4. by the seedling directly transplanting cleaned in the mixed-matrix of red soil and sand, by weight, red soil: sand=2:1, irrigates normal root water, and sprays tpn and carry out matrix sterilization; 5. transplant in 6 ~ lO days, suitably should shade, avoid direct sunlight, and note a small amount of ventilation, temperature remains on 25 ~ 28 DEG C;
Three, plant when plantation (1) seedling grows to 6 ~ 8 true leaves, select good, the anosis undamaged nursery stock of robust plant, root system development; (2) planting seeding row spacing is 40cm × 50cm; (3) plant rosemary, Xue MingRosma rinus officinalis select the cloudy day, the rainy day and early, late sunlight not strong time; (4) pinching during stem height 15cm after slow seedling;
Four, field management
1. intertill and clean tillage: rosemary, Xue MingRosma rinus officinalis intertills 2 ~ 3 times every year, the time, keep the ventilative not ponding of loosing soil, each intertillage, all in conjunction with weeding, fertilising and pruning, kept native face without weeds respectively in 3 ~ April, 7 ~ August and 10 ~ November;
2. irrigation and drainage: 1. water 1 time according to soil moisture content every 7 ~ lO days early stage; 2. middle and later periods thread is closed weather conditions and soil and is irrigated in good time, forbids flood irrigation and field ponding;
3. Pruning and rectifying: prune after 1. rosemary, Xue MingRosma rinus officinalis plantation survives latter 3 months, do not exceed the half of branch length during each pruning; 2. renewal pruning is carried out when lO ~ ll month from rhizome portion;
4. fertilizing management: 1. rosemary, Xue MingRosma rinus officinalis can not be high fertile, at Seedling Stage according to soil condition sealing fertilizer after intertill and clean tillage; 2. within 1 month, spray 1 special fertilizer, special fertilizer is mainly Taiwan and produces Huolisu; 3. impose 1 quick-acting fertilizer after each harvesting, based on nitrogen, phosphate fertilizer, topdress and take a small amount of principle repeatedly; 4. urea l50kg/hm is executed respectively early stage at rosemary, Xue MingRosma rinus officinalis 2 summit of growths
2with phosphate fertilizer 30kg/hm
2, by the mode of spreading manuer in holes every strain;
5. the extermination of disease and insect pest: should in line with the timely spraying pesticide of principle controlled early, control less, controlled; (1) rosemary, Xue MingRosma rinus officinalis gray mold prophylactico-therapeutic measures: with 5% carbendazim fumigant or the 1500 times of liquid controls of 50% Sukeling; (2) rosemary, Xue MingRosma rinus officinalis controlling powdery mildew measure 20% triazole copper missible oil, 2000 times of liquid controls; (3) rosemary, Xue MingRosma rinus officinalis stem rot prophylactico-therapeutic measures 50% carbendazim or thiophanate methyl 2.00 ~ 1.25ml/L liquid sprays; (4) rosemary, Xue MingRosma rinus officinalis control of aphids measure adopts 5% to flutter lice aphid 2500 times of liquid and sprays control; (5) prophylactico-therapeutic measures of rosemary, Xue MingRosma rinus officinalis trialeurodes vaporariorum adopts 1.5% Avermectin, 3000 times of liquid to spray control;
Five, gather
1. rosemary, Xue MingRosma rinus officinalis young sprout stops growing, and blade is thickening, and color is dark green, gathers during more than plant height 80cm;
2. be rosemary, Xue MingRosma rinus officinalis flowering time for making the collecting time of tealeaves, position of gathering is the position of flower and stem apex band tender leaf;
3. for extracting about the rosemary, Xue MingRosma rinus officinalis collecting time of antioxidant is October; Adopt old branch, stay spray.
Beneficial effect of the present invention is: Producing medicinal herbs is the source of Chinese medicine development, production, development and application, realize the modernization of Chinese medicine, internationalization, promote that traditional Chinese medicine industry is to the development of modern TCM industry, improve the competitiveness of Chinese medicine in international market, quality is core, therefore, Producing medicinal herbs standardization (GAP) and industrialization become the inevitable requirement of Development of Traditional Chinese Medicine, adopt said method scheme, comprise seed selection, best planting period, plantation density, fertilizing method, pest control method, field management, optimal harvest time and collecting method; Can effectively solve Producing medicinal herbs On The Standardization, to guarantee leaf rosemary, Xue MingRosma rinus officinalis safe, efficient, stable, quality controllablely have extremely important effect;
This achievement belongs to traditional Chinese medicine GAP implantation methods field, meet regulation and the requirement of " Good Agricultural Practices (GAP) (the trying) " and " Good Agricultural Practices management measures for certification " that national drug food Surveillance Authority issues, be easy to realize quality control during spread plantation, the leaf Rosmarinus produced according to the present invention is in healthful Chinese crud drug, and product quality meets following specifications documentation requirements:
1,2010 editions Pharmacopoeia of the People's Republic of China standards;
2, " green professional standard imported and exported by medicinal plant and preparation ";
3, " green Chinese medicine outlet manufacturing enterprise's professional standard (trying);
In sum, " the GAP cultivation method in leaf rosemary, Xue MingRosma rinus officinalis garden " can improve seed output and quality effectively, promotes the standardization and industrialization development that leaf rosemary, Xue MingRosma rinus officinalis is planted; Be conducive to environmental protection and the ecological balance, prevent erosion, realize the sustainable use of rosemary leave resource; Planted by standardization, rosemary, Xue MingRosma rinus officinalis that is intensive, industrialization, produce good economic benefits and ecological environment benefit, promote rosemary, Xue MingRosma rinus officinalis industrial upgrading.
Below in conjunction with embodiment, the present invention will be further described.
Embodiment
The GAP cultivation method in leaf rosemary, Xue MingRosma rinus officinalis garden, comprise the Construction and management in leaf rosemary, Xue MingRosma rinus officinalis garden and leaf rosemary, Xue MingRosma rinus officinalis seedling fostering, planting period, plantation management, plantation density, fertilising, field management, the extermination of disease and insect pest, rosemary leave picking time and collecting method:
One, the leaf construction in rosemary, Xue MingRosma rinus officinalis garden
1, selection of land: select physical features higher, not accumulated water field, mountain soil, dry land;
2, wholely: all turned over by planting site, smash, ploughing depth is 30 centimeters, and the soil ridging turned over has been dug drain ditch; Ridge bar soil face width degree keeps 30 ~ 50 centimeters, the height at the bottom of native face to drain ditch 30 centimeters;
3, land improvement: every mu of farmyard manure 30 ~ 40kg executing to become thoroughly decomposed, phosphate fertilizer 300kg; Then plough deeply, harrow broken, leveling; Bed soil mixes sand, improves the gas permeability of soil, water penetration, strengthens heat-transfer capability;
4, build garden and do road well simultaneously, build up the water conservancy corollary equipments such as irrigation and drainage system;
Two, propagation method
1. seminal propagation: (1) collects seed: rosemary, Xue MingRosma rinus officinalis seed propagation, and in two Room ovarys, only a room is solid, and ripening rate is 11.1%; It is June that seed starts maturation time; After seed collection, 0 DEG C ~ 10 DEG C dry Tibetan in refrigerator; (2) sow: 9 ~ October of sowing time; Smooth furrow face before sowing, after planting, burns soil by dusting cover screen cover 1 layer (about 0.5cm); Then whole furrow face is covered, timely moisturizing with plastic film shed; (3) seed and seedling management: 1. adopt Seedling bag nursery; Sow 15 ~ 25 days seed sproutings, germination temperature is between 20 ~ 24 DEG C; 2. can be transplanted on Seedling bag when seedling grows to 4 ~ 5cm height, in Seedling bag, 40 ~ 60 days time, keep ground moistening; 3. to apply fertilizer to accelerate seedling growth in the second half section in little seedling stage; Clear water drip washing nursery stock blade to be used immediately in order to avoid produce fertilizer damage after each fertilising; 4. before transplanting, seedling at least will carry out the hardening of more than lO days, transplants seedlings before morning 10:00 and after afternoon 4:00; Transplant nursery stock after 5 ~ 7 days to start to send out roots; 5. transplant after 10 days and carry out the 1st fertilising, execute the aqueous solution that 5g/L composite fertilizer is made into better, executed 1 time every 7 ~ 10 days; Can transplant to land for growing field crops when height of seedling reaches 10 ~ 15cm;
2. cottage propagation: the construction of (1) cutting orchard: will select to garden that fertile soil, viscosity are little, good permeability, physical features is mild, and illumination is sufficient, and draining is good, has abundant water resources, has a good transport service, and is convenient to the place that manages; (2) cuttings is selected and process: 1. cuttage branch selects raw, healthy and strong, maternal plant then without the short semi-lignified branch of damage by disease and insect, pitch, length 8 ~ 12cm; 2. longer or stout and strong branch is cut into several sections, and each section has the joint in more than 4 roads; 1 ~ 2, leaf is stayed in upper end, and stay full leaf, all the other are erased, and lower end is cut into horse ear shape; 3. the cuttings got ready puts into the container that clear water is housed, and uses potassium permanganate sterilization, then use root-inducing powder immersion branch growth-promoting root after soaking 5 ~ 10min; (3) seedbed prepares: 1. leeward facing south should be selected in seedbed, and physical features is smooth, near water source, good permeability, and the sand not easily hardened after watering, high bed is taked in seedbed; 2. within first 1 day, with the liquor potassic permanganate of 1g/L, seedbed is carried out disinfection in cuttage, prepare the front seedbed of cuttage and will to drench water; (4) cutting medium: rosemary, Xue MingRosma rinus officinalis cutting medium selects one of following each mixture: the mixture of the mixture of the mixture of the mixture of peat soil and perlitic mixture, plant ash and river sand soil, sandy loam, perlite and river sand soil, peat soil and loess, peat soil and river sand soil; (5) cutting time and method: 1. the rosemary, Xue MingRosma rinus officinalis cuttage and seedling culture time is 3 ~ April, cutting time at the cloudy day or should carry out afternoon; 2. during cuttage, cuttings is inserted the degree of depth 3 ~ 4cm in soil, insert 2 joints in soil, seeding row spacing 5cm × 5cm; 3. before cuttage, seedbed water is watered; Will water permeable after insertion in time, first time waters and adopts the method for spray, finds that seedling wants timely righting, solid steady, the moisturizing of upper cover plastic film; (6) cuttage seeding management: 1. cuttage seeding is in two weeks, must water every day 1 time, watering time is selected early or evening; Sunlight is strong, temperature height time to shade, irrigation times also will increase; 2., after two weeks, cuttage seeding starts to take root; After cuttings cuttage every 7 days spray 1 time 1500 times chlorothalonil or thunder many Mils bactericide, all to add the potassium dihydrogen phosphate of 0.2% in two kinds of bactericide, cuttings resistance can be strengthened; 3. can open film gradually after cuttage lO days, after 20 ~ 25 days, film all be opened; 4. rosemary, Xue MingRosma rinus officinalis cuttage and seedling culture stays the garden time to need 90 days; Treat that seedling growth is healthy and strong, well developed root system, can transplant when height of seedling reaches more than 10cm; 5. getting the front seedbed of seedling waters permeable, gets Miao Shiying and has digged with a hoe, and allows soil adhere to root system, and is bundled into wisp with straw, to transplant;
3. tissue culture propagation: (1) explant kind and process: 1. the rosemary, Xue MingRosma rinus officinalis explant of tissue cultures comprises blade, stem apex, the stem section on annual spray, its processing method of different explants is different;
(2) blade: 1. detergent liquid first soaks 1h, 75% alcohol-pickled 30s after running water 1h; 2. 17min is soaked, aseptic water washing 3 ~ 4 times with saturated calcium hypochlorite solution again;
(3) stem apex: 1. get greenhouse pot culture rosemary, Xue MingRosma rinus officinalis terminal bud, be placed in running water and rinse 30min, then use the alcohol-pickled 10s of 75%, after aseptic water washing 3 times, add 0.1g/L HgCl
2solution soaks 5min, and often liter of solution adds tween 2; 2. use aseptic water washing 5-6 time, flushing process will constantly stir; 3. the material of sterilizing is put into sterile beaker when aseptic;
(4) stem section: the branch choosing robust growth, removes blade, drops in washing powder solution and cleans 2 times, and aseptic water washing for several times, then soaks in the clever solution of the many shades of 20ml/L;
(5) sterile working on workbench: the 1. HgCl of the 1st sterilization 1g/L
2solution leaching 3min, with aseptic washing 2 times; 2. the 2nd sterilization is carried out after, with the HgCl of 1g/L
2solution leaching 2min, then with aseptic washing 5 times; 3. the material disinfected is cut into little length of tape 2 ~ 3 bud that 2.0 ~ 3.Ocm is long;
(6) medium is selected: medium is 1/2MS+ sucrose 1.5%+IBAl.0mg/L+NAAO.5mg/L+PP3330.05mg/L;
(7) plantlet in vitro management: 1. transplant and select in spring; 2. the test-tube plantlet of 3 ~ 4 leaves there is is to move to hardening canopy 7 ~ 10 days long for root 5 ~ 6cm; 3. being poured on by bottle seedling fills in the large basin of running water, washes away basifixed medium gently, notes not damaging root system and cauline leaf; 4. by the seedling directly transplanting cleaned in the mixed-matrix of red soil and sand, by weight, red soil: sand=2:1, irrigates normal root water, and sprays tpn and carry out matrix sterilization; 5. transplant in 6 ~ lO days, suitably should shade, avoid direct sunlight, and note a small amount of ventilation, temperature remains on 25 ~ 28 DEG C;
Three, plant when plantation (1) seedling grows to 6 ~ 8 true leaves, select good, the anosis undamaged nursery stock of robust plant, root system development; (2) planting seeding row spacing is 40cm × 50cm; (3) plant rosemary, Xue MingRosma rinus officinalis select the cloudy day, the rainy day and early, late sunlight not strong time; (4) pinching during stem height 15cm after slow seedling, inspires side shoot;
Four, field management
1. intertill and clean tillage: rosemary, Xue MingRosma rinus officinalis intertills 2 ~ 3 times every year, the time, keep the ventilative not ponding of loosing soil, each intertillage, all in conjunction with weeding, fertilising and pruning, kept native face without weeds respectively in 3 ~ April, 7 ~ August and 10 ~ November;
2. irrigation and drainage: 1. water 1 time according to soil moisture content every 7 ~ lO days early stage; 2. middle and later periods thread is closed weather conditions and soil and is irrigated in good time, forbids flood irrigation and field ponding;
3. Pruning and rectifying: prune after 1. rosemary, Xue MingRosma rinus officinalis plantation survives latter 3 months, do not exceed the half of branch length during each pruning, in order to avoid affect the regeneration capacity of plant; 2. rosemary, Xue MingRosma rinus officinalis is after the plantation several years, and the strain shape of plant can become deflection, carries out renewal pruning when lO ~ ll month from rhizome portion;
4. fertilizing management: 1. rosemary, Xue MingRosma rinus officinalis can not be high fertile, at Seedling Stage according to soil condition sealing fertilizer after intertill and clean tillage; 2. within 1 month, spray 1 special fertilizer, special fertilizer is mainly Taiwan and produces Huolisu; 3. impose 1 quick-acting fertilizer after each harvesting, based on nitrogen, phosphate fertilizer, topdress and take a small amount of principle repeatedly; 4. urea l50kg/hm is executed respectively early stage at rosemary, Xue MingRosma rinus officinalis 2 summit of growths
2with phosphate fertilizer 30kg/hm
2, by the mode of spreading manuer in holes every strain;
5. the extermination of disease and insect pest: should in line with the timely spraying pesticide of principle controlled early, control less, controlled; (1) rosemary, Xue MingRosma rinus officinalis gray mold prophylactico-therapeutic measures: with 5% carbendazim fumigant or the 1500 times of liquid controls of 50% Sukeling; (2) rosemary, Xue MingRosma rinus officinalis controlling powdery mildew measure 20% triazole copper missible oil, 2000 times of liquid controls; (3) rosemary, Xue MingRosma rinus officinalis stem rot prophylactico-therapeutic measures 50% carbendazim or thiophanate methyl 2.00 ~ 1.25ml/L liquid sprays; (4) rosemary, Xue MingRosma rinus officinalis control of aphids measure adopts 5% to flutter lice aphid 2500 times of liquid and sprays control; (5) prophylactico-therapeutic measures of rosemary, Xue MingRosma rinus officinalis trialeurodes vaporariorum adopts 1.5% Avermectin, 3000 times of liquid to spray control;
Five, gather
1. rosemary, Xue MingRosma rinus officinalis young sprout stops growing, and blade is thickening, and color is dark green, gathers during more than plant height 80cm;
2. be rosemary, Xue MingRosma rinus officinalis flowering time for making the collecting time of tealeaves, position of gathering is the position of flower and stem apex band tender leaf;
3. for extracting about the rosemary, Xue MingRosma rinus officinalis collecting time of antioxidant is October; Adopt old branch, stay spray.
Claims (1)
1. the leaf GAP cultivation method in rosemary, Xue MingRosma rinus officinalis garden, comprise the Construction and management in leaf rosemary, Xue MingRosma rinus officinalis garden and leaf rosemary, Xue MingRosma rinus officinalis seedling fostering, planting period, plantation management, plantation density, fertilising, field management, the extermination of disease and insect pest, rosemary leave picking time and collecting method:
One, the leaf construction in rosemary, Xue MingRosma rinus officinalis garden
1, selection of land: select physical features higher, not accumulated water field, mountain soil, dry land;
2, wholely: all turned over by planting site, smash, ploughing depth is 30 centimeters, and the soil ridging turned over has been dug drain ditch; Ridge bar soil face width degree keeps 30 ~ 50 centimeters, the height at the bottom of native face to drain ditch 30 centimeters;
3, land improvement: every mu of farmyard manure 30 ~ 40kg executing to become thoroughly decomposed, phosphate fertilizer 300kg; Then plough deeply, harrow broken, leveling; Bed soil mixes sand;
Two, propagation method
1. seminal propagation: (1) collects seed: rosemary, Xue MingRosma rinus officinalis seed propagation, and in two Room ovarys, only a room is solid, and ripening rate is 11.1%; It is June that seed starts maturation time; After seed collection, 0 DEG C ~ 10 DEG C dry Tibetan in refrigerator; (2) sow: 9 ~ October of sowing time; Smooth furrow face before sowing, after planting, burns soil by dusting cover screen cover 1 layer; Then whole furrow face is covered, timely moisturizing with plastic film shed; (3) seed and seedling management: 1. adopt Seedling bag nursery; Sow 15 ~ 25 days seed sproutings, germination temperature is between 20 ~ 24 DEG C; 2. can be transplanted on Seedling bag when seedling grows to 4 ~ 5cm height, in Seedling bag, 40 ~ 60 days time, keep ground moistening; 3. to apply fertilizer in the second half section in little seedling stage; Clear water drip washing nursery stock blade to be used immediately in order to avoid produce fertilizer damage after each fertilising; 4. before transplanting, seedling at least will carry out the hardening of lO days, transplants seedlings before morning 10:00 and after afternoon 4:00; Transplant nursery stock after 5 ~ 7 days to start to send out roots; 5. transplant after 10 days and carry out the 1st fertilising, execute the aqueous solution that 5g/L composite fertilizer is made into better, executed 1 time every 7 ~ 10 days; Can transplant to land for growing field crops when height of seedling reaches 10 ~ 15cm;
2. cottage propagation: the construction of (1) cutting orchard: will select to garden that fertile soil, viscosity are little, good permeability, physical features is mild, and illumination is sufficient, and draining is good, has abundant water resources, has a good transport service, and is convenient to the place that manages; (2) cuttings is selected and process: 1. cuttage branch selects raw, healthy and strong, maternal plant then without the short semi-lignified branch of damage by disease and insect, pitch, length 8 ~ 12cm; 2. longer or stout and strong branch is cut into several sections, and each section has the joint in more than 4 roads; 1 ~ 2, leaf is stayed in upper end, and stay full leaf, all the other are erased, and lower end is cut into horse ear shape; 3. the cuttings got ready puts into the container that clear water is housed, and uses potassium permanganate sterilization, then use root-inducing powder immersion branch growth-promoting root after soaking 5 ~ 10min; (3) seedbed prepares: 1. leeward facing south should be selected in seedbed, and physical features is smooth, near water source, good permeability, and the sand not easily hardened after watering, high bed is taked in seedbed; 2. within first 1 day, with the liquor potassic permanganate of 1g/L, seedbed is carried out disinfection in cuttage, prepare the front seedbed of cuttage and will to drench water; (4) cutting medium: rosemary, Xue MingRosma rinus officinalis cutting medium selects one of following each mixture: the mixture of the mixture of the mixture of the mixture of peat soil and perlitic mixture, plant ash and river sand soil, sandy loam, perlite and river sand soil, peat soil and loess, peat soil and river sand soil; (5) cutting time and method: 1. the rosemary, Xue MingRosma rinus officinalis cuttage and seedling culture time is 3 ~ April, cutting time at the cloudy day or should carry out afternoon; 2. during cuttage, cuttings is inserted the degree of depth 3 ~ 4cm in soil, insert 2 joints in soil, seeding row spacing 5cm × 5cm; 3. before cuttage, seedbed water is watered; Will water permeable after insertion in time, first time waters and adopts the method for spray, finds that seedling wants timely righting, solid steady, the moisturizing of upper cover plastic film; (6) cuttage seeding management: 1. cuttage seeding is in two weeks, must water every day 1 time, watering time is selected early or evening; Sunlight is strong, temperature height time to shade, irrigation times also will increase; 2., after two weeks, cuttage seeding starts to take root; After cuttings cuttage every 7 days spray 1 time 1500 times chlorothalonil or thunder many Mils bactericide, all to add the potassium dihydrogen phosphate of 0.2% in two kinds of bactericide, cuttings resistance can be strengthened; 3. can open film gradually after cuttage lO days, after 20 ~ 25 days, film all be opened; 4. rosemary, Xue MingRosma rinus officinalis cuttage and seedling culture stays the garden time to need 90 days; Treat that seedling growth is healthy and strong, well developed root system, can transplant when height of seedling reaches more than 10cm; 5. getting the front seedbed of seedling waters permeable, gets Miao Shiying and has digged with a hoe, and allows soil adhere to root system, and is bundled into wisp with straw, to transplant;
3. tissue culture propagation: (1) explant kind and process: 1. the rosemary, Xue MingRosma rinus officinalis explant of tissue cultures comprises blade, stem apex, the stem section on annual spray, its processing method of different explants is different;
(2) blade: 1. detergent liquid first soaks 1h, 75% alcohol-pickled 30s after running water 1h; 2. 17min is soaked, aseptic water washing 3 ~ 4 times with saturated calcium hypochlorite solution again;
(3) stem apex: 1. get greenhouse pot culture rosemary, Xue MingRosma rinus officinalis terminal bud, be placed in running water and rinse 30min, then use the alcohol-pickled 10s of 75%, after aseptic water washing 3 times, add 0.1g/L HgCl
2solution soaks 5min, and often liter of solution adds tween 2; 2. use aseptic water washing 5 ~ 6 times, flushing process will constantly stir; 3. the material of sterilizing is put into sterile beaker when aseptic;
(4) stem section: the branch choosing robust growth, removes blade, drops in washing powder solution and cleans 2 times, and aseptic water washing for several times, then soaks in the clever solution of the many shades of 20ml/L;
(5) sterile working on workbench: the 1. HgCl of the 1st sterilization 1g/L
2solution leaching 3min, with aseptic washing 2 times; 2. the 2nd sterilization is carried out after, with the HgCl of 1g/L
2solution leaching 2min, then with aseptic washing 5 times; 3. the material disinfected is cut into little length of tape 2 ~ 3 bud that 2.0 ~ 3.Ocm is long;
(6) medium is selected: medium is 1/2MS+ sucrose 1.5%+IBAl.0mg/L+NAAO.5mg/L+PP3330.05mg/L;
(7) plantlet in vitro management: 1. transplant and select in spring; 2. the test-tube plantlet of 3 ~ 4 leaves there is is to move to hardening canopy 7 ~ 10 days long for root 5 ~ 6cm; 3. being poured on by bottle seedling fills in the large basin of running water, washes away basifixed medium gently, notes not damaging root system and cauline leaf; 4. by the seedling directly transplanting cleaned in the mixed-matrix of red soil and sand, by weight, red soil: sand=2:1, irrigates normal root water, and sprays tpn and carry out matrix sterilization; 5. transplant in 6 ~ lO days, suitably should shade, avoid direct sunlight, and note a small amount of ventilation, temperature remains on 25 ~ 28 DEG C;
Three, plant when plantation (1) seedling grows to 6 ~ 8 true leaves, select good, the anosis undamaged nursery stock of robust plant, root system development; (2) planting seeding row spacing is 40cm × 50cm; (3) plant rosemary, Xue MingRosma rinus officinalis select the cloudy day, the rainy day and early, late sunlight not strong time; (4) pinching during stem height 15cm after slow seedling;
Four, field management
1. intertill and clean tillage: rosemary, Xue MingRosma rinus officinalis intertills 2 ~ 3 times every year, the time, keep the ventilative not ponding of loosing soil, each intertillage, all in conjunction with weeding, fertilising and pruning, kept native face without weeds respectively in 3 ~ April, 7 ~ August and 10 ~ November;
2. irrigation and drainage: 1. water 1 time according to soil moisture content every 7 ~ lO days early stage; 2. middle and later periods thread is closed weather conditions and soil and is irrigated in good time, forbids flood irrigation and field ponding;
3. Pruning and rectifying: prune after 1. rosemary, Xue MingRosma rinus officinalis plantation survives latter 3 months, do not exceed the half of branch length during each pruning; 2. renewal pruning is carried out when lO ~ ll month from rhizome portion;
4. fertilizing management: 1. rosemary, Xue MingRosma rinus officinalis can not be high fertile, at Seedling Stage according to soil condition sealing fertilizer after intertill and clean tillage; 2. 1 special fertilizer within 1 month, is sprayed; 3. impose 1 quick-acting fertilizer after each harvesting, based on nitrogen, phosphate fertilizer, topdress and take a small amount of principle repeatedly; 4. urea l50kg/hm is executed respectively early stage at rosemary, Xue MingRosma rinus officinalis 2 summit of growths
2with phosphate fertilizer 30kg/hm
2, by the mode of spreading manuer in holes every strain;
5. the extermination of disease and insect pest: should in line with the timely spraying pesticide of principle controlled early, control less, controlled; (1) rosemary, Xue MingRosma rinus officinalis gray mold prophylactico-therapeutic measures: with 5% carbendazim fumigant or the 1500 times of liquid controls of 50% Sukeling; (2) rosemary, Xue MingRosma rinus officinalis controlling powdery mildew measure 20% triazole copper missible oil, 2000 times of liquid controls; (3) rosemary, Xue MingRosma rinus officinalis stem rot prophylactico-therapeutic measures 50% carbendazim or thiophanate methyl 2.00 ~ 1.25ml/L liquid sprays; (4) rosemary, Xue MingRosma rinus officinalis control of aphids measure adopts 5% to flutter lice aphid 2500 times of liquid and sprays control; (5) prophylactico-therapeutic measures of rosemary, Xue MingRosma rinus officinalis trialeurodes vaporariorum adopts 1.5% Avermectin, 3000 times of liquid to spray control;
Five, gather
1. rosemary, Xue MingRosma rinus officinalis young sprout stops growing, and blade is thickening, and color is dark green, gathers during more than plant height 80cm;
2. be rosemary, Xue MingRosma rinus officinalis flowering time for making the collecting time of tealeaves, position of gathering is the position of flower and stem apex band tender leaf;
3. for extracting about the rosemary, Xue MingRosma rinus officinalis collecting time of antioxidant is October; Adopt old branch, stay spray.
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