CN104230947A - Preparation method for extracting psoralen from radix fici simplicissimae - Google Patents
Preparation method for extracting psoralen from radix fici simplicissimae Download PDFInfo
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- CN104230947A CN104230947A CN201310229797.8A CN201310229797A CN104230947A CN 104230947 A CN104230947 A CN 104230947A CN 201310229797 A CN201310229797 A CN 201310229797A CN 104230947 A CN104230947 A CN 104230947A
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- fici simplicissimae
- psoralene
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- ethanol
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D493/00—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system
- C07D493/02—Heterocyclic compounds containing oxygen atoms as the only ring hetero atoms in the condensed system in which the condensed system contains two hetero rings
- C07D493/04—Ortho-condensed systems
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention relates to the field of Chinese herbal medicine formula, and specifically relates to a preparation method for extracting psoralen from radix fici simplicissimae. The preparation method for psoralen comprises the steps of extracting from a medicinal plant-radix fici simplicissimae, concentrating and drying. According to a technical solution provided by the invention, a main component of psoralen contained in a radix fici simplicissimae medicinal material has a plurality of pharmacological effects such as antibiosis, antivirus, tumor inhibition, anticoagulation, immune regulation and tufsin. The radix fici simplicissimae extract of psoralen can be produced in a large scale easily and has no pollution to environment.
Description
Technical field:
The present invention relates to vegetable Chinese herbal medicine formulation art, be specifically related to a kind of preparation method extracting psoralene from Herba Fici Simplicissimae.
Technical background:
Herba Fici Simplicissimae is the root of Moraceae ficus species ficus simplicissima (Ficus simplicissima Lour.), and can strengthening the spleen and tonifying the lung, promoting the circulation of qi dampness removing, stimulates the circulation of the blood and cause the muscles and joints to relax.Be used for the treatment of insufficiency of the spleen edema, food is few unable, pulmonary tuberculosis cough, night sweat, under band, postpartum agalasisa, rheumatic arthralgia, oedema, hepatic ascites, hepatitis, wound etc.Main component contained by Herba Fici Simplicissimae medicinal material is Coumarins, sterol, triterpenes, amino acid, carbohydrate etc. according to the literature, wherein, psoralene is furocoumarin compound, because having the multiple pharmacological effect such as antibacterial, antiviral, Tumor suppression, anti-freezing, immunomodulatory, hormonelike, and as the principle active component of Herba Fici Simplicissimae, and document and patent there is no the report of the preparation method extracting psoralene from Herba Fici Simplicissimae at present.
Summary of the invention:
The object of the invention is, in order to provide a kind of low cost, easy to operate, good stability, be applicable to the method preparing psoralene of producing, to disclose a kind of preparation method extracting psoralene from Herba Fici Simplicissimae.This psoralene be extract from medicinal plant Herba Fici Simplicissimae, concentrated, drying forms.
By the experimental study to medicinal plant Herba Fici Simplicissimae, find that the psoralene extracted from Herba Fici Simplicissimae has the multiple pharmacological effect such as antibacterial, antiviral, Tumor suppression, anti-freezing, immunomodulatory, hormonelike.
Embodiment
A kind of preparation method extracting psoralene from Herba Fici Simplicissimae of the present invention is:
(1) get Herba Fici Simplicissimae, after drying, pulverizing, cross 40 to 60 mesh sieves, then extract with the alcohol heating reflux that alcohol concn is 50-70%, reflux time preferred 1-3 hour, preferred 2-4 time of extraction time, after concentrated, reclaim ethanol;
(2) ethanol of concentration and recovery is added water ethanol concentrated solution is dissolved completely, leave standstill, filter, then filtrate is concentrated for subsequent use;
(3) adopted by filtrate concentrated solution DM101 type macroporous adsorbent resin to wash, discarding water lotion, is 30-70% ethanol elution by concentration, retains elutriant, reclaims ethanol, concentrated, dry, namely prepares psoralene.
According to the technical scheme of above-mentioned preparation method, main component psoralene contained by Herba Fici Simplicissimae medicinal material has the multiple pharmacological effect such as antibacterial, antiviral, Tumor suppression, anti-freezing, immunomodulatory, hormonelike.This Radix Fici Simplicissimae extract psoralene easily realizes suitability for industrialized production, and environmentally safe.
Embodiment one.
Get without Herba Fici Simplicissimae 200g, pulverize, cross 40-60 mesh sieve, with 70% alcohol reflux 2 times, each 2 hours, filter, filtrate is concentrating under reduced pressure in heating in water bath, reclaimed ethanol extremely without alcohol taste, adding suitable quantity of water makes dissolving complete, leaves standstill, filters, filtrate concentrates for subsequent use, then by macroporous adsorption resin chromatography post, first rinses with water, use 30% ethanol elution again, collect ethanol eluate, concentrated, reclaim ethanol, 80 DEG C of dryings, obtain psoralene.
Embodiment two.
Get without Herba Fici Simplicissimae 300g, pulverize, cross 40-60 mesh sieve, with 70% alcohol reflux 3 times, each 1.5 hours, filter, filtrate is concentrating under reduced pressure in heating in water bath, reclaimed ethanol extremely without alcohol taste, adding suitable quantity of water makes dissolving complete, leaves standstill, filters, filtrate concentrates for subsequent use, then by macroporous adsorption resin chromatography post, first rinses with water, use 50% ethanol elution again, collect ethanol eluate, concentrated, reclaim ethanol, 80 DEG C of dryings, obtain psoralene.
Embodiment three
Get without Herba Fici Simplicissimae 400g, pulverize, cross 40-60 mesh sieve, with 70% alcohol reflux 3 times, each 2 hours, filter, filtrate is concentrating under reduced pressure in heating in water bath, reclaimed ethanol extremely without alcohol taste, adding suitable quantity of water makes dissolving complete, leaves standstill, filters, filtrate concentrates for subsequent use, then by macroporous adsorption resin chromatography post, first rinses with water, use 60% ethanol elution again, collect ethanol eluate, concentrated, reclaim ethanol, 80 DEG C of dryings, obtain psoralene.
Embodiment four
Get without Herba Fici Simplicissimae 500g, pulverize, cross 40-60 mesh sieve, with 70% alcohol reflux 4 times, each 1 hour, filter, filtrate is concentrating under reduced pressure in heating in water bath, reclaimed ethanol extremely without alcohol taste, adding suitable quantity of water makes dissolving complete, leaves standstill, filters, filtrate concentrates for subsequent use, then by macroporous adsorption resin chromatography post, first rinses with water, use 70% ethanol elution again, collect ethanol eluate, concentrated, reclaim ethanol, 80 DEG C of dryings, obtain psoralene.
Claims (2)
1. from Herba Fici Simplicissimae, extract a preparation method for psoralene, it is characterized in that: this psoralene be extract from medicinal plant Herba Fici Simplicissimae, concentrated, drying forms.
2. a kind of preparation method extracting psoralene from Herba Fici Simplicissimae according to claim 1, is characterized in that: a kind of preparation method extracting psoralene from Herba Fici Simplicissimae is:
(1) get Herba Fici Simplicissimae, after drying, pulverizing, cross 40 to 60 mesh sieves, then extract with the alcohol heating reflux that alcohol concn is 50-70%, reflux time preferred 1-3 hour, preferred 2-4 time of extraction time, after concentrated, reclaim ethanol;
(2) ethanol of concentration and recovery is added water ethanol concentrated solution is dissolved completely, leave standstill, filter, then filtrate is concentrated for subsequent use;
(3) adopted by filtrate concentrated solution DM101 type macroporous adsorbent resin to wash, discarding water lotion, is 30-70% ethanol elution by concentration, retains elutriant, reclaims ethanol, concentrated, dry, namely prepares psoralene.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310229797.8A CN104230947A (en) | 2013-06-09 | 2013-06-09 | Preparation method for extracting psoralen from radix fici simplicissimae |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310229797.8A CN104230947A (en) | 2013-06-09 | 2013-06-09 | Preparation method for extracting psoralen from radix fici simplicissimae |
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| CN104230947A true CN104230947A (en) | 2014-12-24 |
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| CN201310229797.8A Pending CN104230947A (en) | 2013-06-09 | 2013-06-09 | Preparation method for extracting psoralen from radix fici simplicissimae |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105998980A (en) * | 2016-07-12 | 2016-10-12 | 灏逛寒 | Anti-virus traditional Chinese medicinal composition and preparation method thereof |
| CN114259514A (en) * | 2022-02-18 | 2022-04-01 | 暨南大学附属第一医院（广州华侨医院） | Preparation method of hispid fig extract and application of hispid fig extract in preparation of products for preventing and treating obesity, hyperlipidemia and non-alcoholic steatohepatitis |
| CN115380825A (en) * | 2022-09-20 | 2022-11-25 | 广西壮族自治区药用植物园 | Hispid fig tissue culture method and psoralen preparation method |
-
2013
- 2013-06-09 CN CN201310229797.8A patent/CN104230947A/en active Pending
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105998980A (en) * | 2016-07-12 | 2016-10-12 | 灏逛寒 | Anti-virus traditional Chinese medicinal composition and preparation method thereof |
| CN114259514A (en) * | 2022-02-18 | 2022-04-01 | 暨南大学附属第一医院（广州华侨医院） | Preparation method of hispid fig extract and application of hispid fig extract in preparation of products for preventing and treating obesity, hyperlipidemia and non-alcoholic steatohepatitis |
| CN115380825A (en) * | 2022-09-20 | 2022-11-25 | 广西壮族自治区药用植物园 | Hispid fig tissue culture method and psoralen preparation method |
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Application publication date: 20141224 |