CN104186314B - A kind of method for culturing seedlings of Herba Anoectochili roxburghii - Google Patents
A kind of method for culturing seedlings of Herba Anoectochili roxburghii Download PDFInfo
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Abstract
The invention discloses the method for culturing seedlings of a kind of Herba Anoectochili roxburghii, including: step one, choose robust plant, vein and clearly and cross the Herba Anoectochili roxburghii at florescence, take its on the ground and between under ground portion 2～4cm one section of stem, remainder is smashed to pieces formation solution, in solution, add 1%～2% polysorbas20, afterwards stem is placed in solution, process 24～72h at 4 DEG C；Step 2, the seedling after the stem processed in step one being placed in 1/3～1/2MS cultured on solid medium 10～being taken root for 15 days, use Full daylight to shine during cultivation；Step 3, seedling step 2 obtained moves to cultivate 50～60 days in 1/4～1/3MS fluid medium, and is joined in MS fluid medium by the Herba Anoectochili roxburghii solution in step one；Step 4, is loaded on solid matrix in the cup-like containers that degradation material is made, and cultivates 20～40 days in seedling replanting step 3 obtained to described solid matrix.
Description
Technical field
The present invention relates to the method for culturing seedlings of a kind of Herba Anoectochili roxburghii.
Background technology
Herba Anoectochili roxburghii is the orchid family Anoectochilus Blume Anoectochilus Roxburghii, has significant heat-clearing and toxic substances removing and dispels the wind the wet removing heat from blood of sleeve
Effect that suppressing the hyperactive liver reinforces the kidney, is the most rare famous and precious Chinese crude drug.Among the people, there is extensive medical value, element
There are the laudatory titles such as " king of medicine ", " gold grass ", " SHENCAO ", " bird Radix Ginseng ".Herba Anoectochili roxburghii is typical negative medicinal
Plant, is frequently grown in the fertile leaf mould under the broad-leaf forest that the moon covers, to air humidity in woods, illumination,
The ecological conditions such as accumulated temperature require strict.Herba Anoectochili roxburghii demand surges in recent years, and wild producing region is due to people's mistake
Degree is excavated so that it is resource is the most exhausted.
Summary of the invention
The present invention provides the method for culturing seedlings of a kind of Herba Anoectochili roxburghii, and the present invention is fast and safely cultivated by tissue culture technology
Herba Anoectochili roxburghii seedling, by successively cultivating Herba Anoectochili roxburghii seedling in MS culture medium and solid matrix, obtains
Seedling can directly be transplanted in soil, it is not necessary to transplants, and solves Herba Anoectochili roxburghii rapid, high volume breeding seedling
The problem low with survival rate after kind of transplantation of seedlings.
The technical scheme that the present invention provides is:
A kind of method for culturing seedlings of Herba Anoectochili roxburghii, including:
Step one, chooses robust plant, vein and clearly and crosses the Herba Anoectochili roxburghii at florescence, cross the gold at florescence
The base of the plant of Anoectochilus roxburghii is more sturdy, takes one section of master of 2 between its aerial parts and under ground portion～4cm
Stem, smashs formation Herba Anoectochili roxburghii solution to pieces by remainder, adds 1%～2% tween～20 in Herba Anoectochili roxburghii solution,
Afterwards described stem is placed in Herba Anoectochili roxburghii solution, processes 24～72h at 4 DEG C, the aminoacid in Herba Anoectochili roxburghii
The highest with the content of trace element, taurine, polysaccharide composition contained by it also can preferably keep stem
Activity, the biological activity that can make stem at 4 DEG C of process stems is close so that it is when taking root, rooting rate is more
Unanimously；
Step 2, is placed on 1/3～1/2MS solid medium raw by the described stem processed in step one
Growing the seedling after being taken root for 10～15 days, use Full daylight photograph during cultivation, intensity of illumination is
1000～1500Lux, cultivation temperature is 24 DEG C～28 DEG C, uses low intensive Full daylight photograph during cultivation,
Both avoided the Herba Anoectochili roxburghii caused because illumination is too strong to take root comparatively fast, grow thin and delicate phenomenon, again can be quick
Promote the growth of Herba Anoectochili roxburghii, shorten its growth cycle；
Step 3, seedling step 2 obtained moves to cultivate in 1/4～1/3MS fluid medium 50～60
My god, and the Herba Anoectochili roxburghii solution in step one is joined in MS fluid medium, use long day during cultivation
The irradiation cycle irradiates, bright phase 16h illumination, and intensity of illumination is 2000～2500Lux, and temperature is 28 DEG C,
Dark phase 8h, temperature is 22 DEG C；It is clear that long-day irradiates lower Herba Anoectochili roxburghii vein, and leaf margin is smooth.
Step 4, is loaded on solid matrix in the cup-like containers that degradation material is made, step 3 is obtained
Seedling replanting to described solid matrix being cultivated 20～40 days, within the 1st～3 day, use identical with step 3
Condition cultivate, be allowed to gradually to adapt in this solid matrix grow.4th day to the 20th～40 day,
Using bright phase 16h illumination, intensity of illumination is 3000～3500Lux, and temperature is 30 DEG C, dark phase 8h, temperature
Degree is 22 DEG C, to Herba Anoectochili roxburghii in cup-like containers, grow into 5～6cm time, can be by it directly at cup-shaped
Under in container, directly the tree shade of booth or natural environment is arrived in plantation, owing to cup-like containers is by degradation material
Making, in growth, cup-like containers can be degraded, and, the root of Herba Anoectochili roxburghii is likely to travel through described
Cup-like containers and grow, on itself growth without impact, the most pollution-free to environment.The now root of Herba Anoectochili roxburghii
Grow is the most flourishing, if it dug out from solid matrix, relatively big to the injury of its root, this
Sample does is of value to its root of protection, and directly moves it to greatly from fluid medium traditionally meanwhile
Compression ring is compared in border, and such way makes the survival rate of Herba Anoectochili roxburghii seedling higher, more can rapidly adapt to new
Growing environment.
Wherein, described solid matrix includes the component of following weight fraction: soil 10～20 parts, Vermiculitum 15～30
Part, humus 3～6 parts, Fructus Musae 10～20 parts, Fructus Mangifera Indicae 1～2 parts, peel of Carcinia mangostana L. 2～4 parts and Hylocereus undatus
Peel 1～2 parts.Humus contains some elements required for growth and development of plants, soil can be improved,
Increase fertility；Banana pulp nutritive value is the highest, containing various trace elements and vitamin, can promote growth,
Strengthen the resistance against diseases of Herba Anoectochili roxburghii；Peel of Carcinia mangostana L. and Hylocereus undatus peel can make the resistance against diseases of Herba Anoectochili roxburghii higher,
Fructus Mangifera Indicae
Preferably, the method for culturing seedlings of described Herba Anoectochili roxburghii, in described step 2, first by described stem
It is processed as the fritter of 0.4～0.6cm, then is homogeneously disposed in MS cultured on solid medium.
Preferably, the method for culturing seedlings of described Herba Anoectochili roxburghii, in described step 2, the 1st to 20 day, profit
Cultivate Herba Anoectochili roxburghii with 1/4MS fluid medium, change a solution every 7～10 days；21st to the 50th～60
My god, utilize 1/3MS fluid medium to cultivate Herba Anoectochili roxburghii, change a solution every 7～10 days.
Preferably, the stem do not taken root in described step 2 and described step 3 camber are less than 2
The seedling of cm joins in described solid matrix after smashing.Containing of aminoacid in Herba Anoectochili roxburghii and trace element
Measuring the highest, taurine contained by it, polysaccharide composition are the abundantest, and the growth to Herba Anoectochili roxburghii plant itself has
There is good Nutrition.
Preferably, in the method for culturing seedlings of described Herba Anoectochili roxburghii, described solid matrix includes that following weight is divided
The component of number: 15 parts of soil, Vermiculitum 18 parts, 4 parts of humus, 15 parts of Fructus Musae, Fructus Mangifera Indicae 1 part, mountain
3 parts of bamboo peel and 1 part of Hylocereus undatus peel.
Preferably, in the method for culturing seedlings of described Herba Anoectochili roxburghii, by described Fructus Musae, Fructus Mangifera Indicae, peel of Carcinia mangostana L.
The granule being less than 40 mesh for particle diameter is pulverized with Hylocereus undatus peel.
Preferably, in the method for culturing seedlings of described Herba Anoectochili roxburghii, in incubation, air humidity is
80%～95%.
Preferably, in the method for culturing seedlings of described Herba Anoectochili roxburghii, in described step 3, use plasma water
Preparation 1/4～1/3MS fluid medium, in such culture medium, various nutritional labelings are easier to be inhaled by Herba Anoectochili roxburghii
Receive.
Preferably, in the method for culturing seedlings of described Herba Anoectochili roxburghii, described cup-like containers is by polylactic acid, poly-second
Any one in enol and polyester material is made.
The invention have the benefit that
Shorten the growth cycle of Herba Anoectochili roxburghii according to the method for the present invention, plant strain growth is superior, improves gold
The transplanting survival rate of Anoectochilus roxburghii, its survival rate is up to more than 99%, and the Herba Anoectochili roxburghii plant height grown up to reaches 16～20cm,
The strain of average weight 3g/, seedling raising process Green is safe, without hormone interpolation, organic cultivation, without chemical fertilizer agriculture
Medicine and hormone, seedling and fresh goods Herba Anoectochili roxburghii is safer, mouthfeel is more preferable.
Detailed description of the invention
The present invention is described in further detail below, to make those skilled in the art with reference to description literary composition
Word can be implemented according to this.
Embodiment 1:
Culture medium:
MS culture medium prescription:
A great number of elements:
1650mg/L NH4NO3, 1900mg/L KNO3, 440mg/L CaCl2 2H2O, 370mg/L
MgSO4·7H2O and 700mg/L KH2PO4；
Trace element:
0.83mg/L KI, 6.25mg/L H3BO3, 22.3mg/LMnSO4·4H2O, 8.65mg/L
ZnSO4·7H2O, 0.25mg/LNa2MnO4·2H2O, 0.025mg/L CuSO4·5H2O and 0.025
mg/LCoCl2·6H2O
Iron salt: 27.8mg/L FeSO4·7H2O and 7.3mg/L Na2-EDTA·2H2O3
Sucrose 30mg/L,
Another addition agar powder 8mg/L,
Tris and MES regulation pH to about 5.80 i.e. obtains MS solid medium.
Separately add agar powder 8mg/L cosmetics without fine jade and be MS fluid medium
1/3MS solid medium: a great number of elements, trace element, iron salt and sugar composition are MS culture medium
1/3, another agar powder 8mg/L, Tris and the MES regulation pH to about 5.80 that adds i.e. obtains 1/3MS
Solid medium.
1/2MS solid medium: a great number of elements, trace element, iron salt and sugar composition are MS culture medium
1/2, another agar powder 8mg/L, Tris and the MES regulation pH to about 5.80 that adds i.e. obtains 1/2MS
Solid medium.
1/4MS fluid medium: a great number of elements, trace element, iron salt and sugar composition are MS culture medium
1/4, Tris and MES regulation pH to about 5.80 i.e. obtain 1/4MS solid medium.
1/3MS fluid medium: a great number of elements, trace element, iron salt and sugar composition are that MS cultivates
The 1/3 of base, Tris and MES regulation pH to about 5.80 i.e. obtains 1/3MS solid medium.
Solid matrix includes the component of following weight fraction: 15 parts of soil, Vermiculitum 18 parts, humus 4
Part, 15 parts of Fructus Musae, Fructus Mangifera Indicae 1 part, peel of Carcinia mangostana L. 3 parts and 1 part of Hylocereus undatus peel, wherein by Fructus Musae,
Fructus Mangifera Indicae, peel of Carcinia mangostana L. and Hylocereus undatus peel are pulverized and are mixed all with other components less than the granule of 40 mesh for particle diameter
Even.
Embodiment 2:
The method for culturing seedlings of a kind of Herba Anoectochili roxburghii, comprises the steps:
1), choose robust plant, vein and clearly and crossed the Herba Anoectochili roxburghii at florescence, take its aerial parts and ground
One section of stem of the 3cm between lower part, smashs formation Herba Anoectochili roxburghii solution to pieces, to Herba Anoectochili roxburghii by remainder
Solution adds 1.5% tween 20, afterwards described stem is placed in Herba Anoectochili roxburghii solution, process 48 at 4 DEG C
h。
2), by step 1) in the described stem that processed be processed as the fritter of 0.5cm, be homogeneously disposed in 1/3
MS cultured on solid medium within 12 days, taken root after seedling, during cultivation use Full daylight photograph, light
Being 1200Lux according to intensity, cultivation temperature is 26 DEG C, and air humidity is 90%.
3), by step 2) seedling that obtains moves to the 1/4MS fluid medium of plasma water preparation and cultivates
20 days, changed a solution every 8 days；From the 21st day, move to the 1/3MS liquid of plasma water preparation
Body culture medium is cultivated to the 55th day, changed a solution every 8 days.And by step 1) in gold thread
Lotus solution joins in 1/4MS fluid medium, uses photoperiod long-day to irradiate, the bright phase 16 during cultivation
H illumination, intensity of illumination is 2300Lux, and temperature is 28 DEG C, dark phase 8h, and temperature is 22 DEG C；Cultivate
During air humidity be 90%.
4), by solid matrix it is loaded in the cup-like containers that polylactic acid is made, by step 3) seedling that obtains
Be transplanted in described solid matrix cultivate 30 days, within the 1st～3 day, use and step 3) in identical condition enter
Row is cultivated, and the 4th day to the 30th day, uses bright phase 16h illumination, and intensity of illumination is 3200Lux, temperature
Degree is 30 DEG C, dark phase 8h, and temperature is 22 DEG C, by step 2) in the stem do not taken root and step 3
The camber seedling less than 2cm joins in solid matrix after smashing.In incubation, air humidity is
90%.
Solid matrix includes the component of following weight fraction: 15 parts of soil, Vermiculitum 18 parts, humus 4
Part, 15 parts of Fructus Musae, Fructus Mangifera Indicae 1 part, peel of Carcinia mangostana L. 3 parts and 1 part of Hylocereus undatus peel, wherein by Fructus Musae,
Fructus Mangifera Indicae, peel of Carcinia mangostana L. and Hylocereus undatus peel are pulverized and are mixed all with other components less than the granule of 40 mesh for particle diameter
Even.
Embodiment 3:
The method for culturing seedlings of a kind of Herba Anoectochili roxburghii, comprises the steps:
1), choose robust plant, vein and clearly and crossed the Herba Anoectochili roxburghii at florescence, take its aerial parts and ground
One section of stem of the 2cm between lower part, smashs formation Herba Anoectochili roxburghii solution to pieces, to Herba Anoectochili roxburghii by remainder
Solution adds 1% tween 20, afterwards stem is placed in Herba Anoectochili roxburghii solution, process 24h at 4 DEG C.
2), by step 1) in the described stem that processed be processed as the fritter of 0.4cm, be homogeneously disposed in 1/3MS
Cultured on solid medium within 10 days, taken root after seedling, during cultivation use Full daylight photograph, illumination is strong
Degree is 1000Lux, and cultivation temperature is 24 DEG C, and air humidity is 80%.
3), by step 2) seedling that obtains moves to the 1/4MS fluid medium of plasma water preparation and cultivates
20 days, changed a solution every 7 days；From the 21st day, move to the 1/3MS liquid of plasma water preparation
Body culture medium is cultivated to the 50th day, changed a solution every 7 days.And by step 1) in gold thread
Lotus solution joins in 1/4MS fluid medium, uses photoperiod long-day to irradiate, the bright phase 16 during cultivation
H illumination, intensity of illumination is 2000Lux, and temperature is 28 DEG C, dark phase 8h, and temperature is 22 DEG C；Cultivate
During air humidity be 80%.
4), by solid matrix it is loaded in the cup-like containers that polyester material is made, by step 3) obtain
Seedling replanting, to cultivating in described solid matrix 20 days, uses and step 3 on the 1st day) in identical condition
Cultivating, the 4th day to the 20th day, use bright phase 16h illumination, intensity of illumination is 3500Lux,
Temperature is 30 DEG C, dark phase 8h, and temperature is 22 DEG C, by step 2) in the stem do not taken root and step 3
The camber seedling less than 2cm joins in described solid matrix after smashing.Air humidity in incubation
It is 80%.
Wherein, solid matrix also includes the component of following parts by weight: 120 parts of soil, Vermiculitum 30 parts,
6 parts of humus, 20 parts of Fructus Musae, Fructus Mangifera Indicae 2 parts, peel of Carcinia mangostana L. 4 parts and 2 parts of Hylocereus undatus peel.
Embodiment 4:
The method for culturing seedlings of a kind of Herba Anoectochili roxburghii, comprises the steps:
1), choose robust plant, vein and clearly and crossed the Herba Anoectochili roxburghii at florescence, take its aerial parts and ground
One section of stem of the 4cm between lower part, smashs formation Herba Anoectochili roxburghii solution to pieces, to Herba Anoectochili roxburghii by remainder
Solution adds 2% tween 20, afterwards described stem is placed in Herba Anoectochili roxburghii solution, process 72h at 4 DEG C.
2), by step 1) in the described stem that processed be processed as the fritter of 0.6cm, be homogeneously disposed in 1/2MS
Cultured on solid medium within 15 days, taken root after seedling, during cultivation use Full daylight photograph, illumination is strong
Degree is 1500Lux, and cultivation temperature is 28 DEG C, and air humidity is 95%.
3), by step 2) seedling that obtains moves to the 1/4MS fluid medium of plasma water preparation and cultivates
20 days, changed a solution every 10 days；From the 21st day, move to the 1/3MS liquid of plasma water preparation
Body culture medium is cultivated to the 60th day, changed a solution every 10 days.And by step 1) in gold
Anoectochilus roxburghii solution joins in 1/4MS fluid medium, uses photoperiod long-day to irradiate, the bright phase during cultivation
16h illumination, intensity of illumination is 2500Lux, and temperature is 28 DEG C, dark phase 8h, and temperature is 22 DEG C；Training
During Yanging, air humidity is 95%.
4), by solid matrix it is loaded in the cup-like containers that polyvinyl alcohol is made, by step 3) children that obtains
Transplantation of seedlings, to cultivating in described solid matrix 40 days, uses and step 3 on the 3rd day) in identical condition enter
Row is cultivated, and the 4th day to the 40th day, uses bright phase 16h illumination, and intensity of illumination is 3000Lux, temperature
Degree is 30 DEG C, dark phase 8h, and temperature is 22 DEG C, by step 2) in the stem do not taken root and step 3
The camber seedling less than 2cm joins in described solid matrix after smashing.Air humidity in incubation
It is 95%.
Wherein, described solid matrix includes the component of following weight fraction: 10 parts of soil, Vermiculitum 15 parts,
3 parts of humus, 10 parts of Fructus Musae, Fructus Mangifera Indicae 1 part, peel of Carcinia mangostana L. 2 parts and 1 part of Hylocereus undatus peel.
Shorten the growth cycle of Herba Anoectochili roxburghii according to the method for the present invention, plant strain growth is superior, improves gold
The transplanting survival rate of Anoectochilus roxburghii, its survival rate is up to more than 99%, and the Herba Anoectochili roxburghii plant height grown up to reaches 16～20cm,
Average weight reaches 3g/ strain.
Although embodiment of the present invention are disclosed as above, but it is not restricted to description and embodiment party
Listed utilization in formula, it can be applied to various applicable the field of the invention completely, for being familiar with ability
For the personnel in territory, be easily achieved other amendment, therefore without departing substantially from claim and etc. homotype
Enclosing under limited general concept, the present invention is not limited to specific details and shown here as the reality with description
Execute example.
Claims (5)
1. the method for culturing seedlings of a Herba Anoectochili roxburghii, it is characterised in that including:
Step one, chooses robust plant, vein and clearly and crosses the Herba Anoectochili roxburghii at florescence, take its aerial parts
And one section of stem of 2～4cm between under ground portion, remainder is smashed to pieces formation Herba Anoectochili roxburghii solution, to
Herba Anoectochili roxburghii solution adds 1%～2% tween 20, afterwards described stem is placed in Herba Anoectochili roxburghii solution, 4
DEG C process 24～72h；
Step 2, is placed on 1/3～1/2MS solid medium raw by the described stem processed in step one
Growing the seedling after being taken root for 10～15 days, use Full daylight photograph during cultivation, intensity of illumination is
1000～1500Lux, cultivation temperature is 24 DEG C～28 DEG C；
Step 3, seedling step 2 obtained moves to cultivate in 1/4～1/3MS fluid medium 50～60
My god, and the Herba Anoectochili roxburghii solution in step one is joined in described 1/4～1/3MS fluid medium, cultivate
Shi Caiyong photoperiod long-day irradiates, bright phase 16h illumination, and intensity of illumination is 2000～2500Lux, temperature
Degree is 28 DEG C, dark phase 8h, and temperature is 22 DEG C；
Step 4, is loaded on solid matrix in the cup-like containers that degradation material is made, step 3 is obtained
Seedling replanting to described solid matrix being cultivated 20～40 days, within the 1st～3 day, use identical with step 3
Condition cultivate, the 4th day to the 20th～40 day, use bright phase 16h illumination, intensity of illumination is
3000～3500Lux, temperature is 30 DEG C, dark phase 8h, and temperature is 22 DEG C,
Wherein, described solid matrix includes the component of following parts by weight: soil 10～20 parts, Vermiculitum 15～30
Part, humus 3～6 parts, Fructus Musae 10～20 parts, Fructus Mangifera Indicae 1～2 parts, peel of Carcinia mangostana L. 2～4 parts and Hylocereus undatus
Peel 1～2 parts；
In incubation, air humidity is 80%～95%,
Described cup-like containers is made up of any one in polylactic acid, polyvinyl alcohol and polyester material；
MS culture medium prescription:
A great number of elements:
1650mg/L NH4NO3, 1900mg/L KNO3, 440mg/L CaCl2·2H2O, 370mg/L
MgSO4·7H2O and 700mg/L KH2PO4；
Trace element:
0.83mg/L KI, 6.25mg/L H3BO3, 22.3mg/LMnSO4·4H2O, 8.65mg/L
ZnSO4·7H2O, 0.25mg/LNa2MnO4·2H2O, 0.025mg/L CuSO4·5H2O and 0.025
mg/LCoCl2·6H2O
Iron salt: 27.8mg/L FeSO4·7H2O and 7.3mg/L Na2-EDTA·2H2O3
Sucrose 30mg/L,
Another addition agar powder 8mg/L,
Tris and MES regulation pH to 5.80 i.e. obtains MS solid medium；
It is MS fluid medium without agar powder 8mg/L；
1/3MS solid medium: a great number of elements, trace element, iron salt and sugar composition are MS culture medium
1/3, another add agar powder 8mg/L, Tris and MES regulation pH to 5.80 i.e. to obtain 1/3MS solid
Body culture medium；
1/2MS solid medium: a great number of elements, trace element, iron salt and sugar composition are MS culture medium
1/2, another add agar powder 8mg/L, Tris and MES regulation pH to 5.80 i.e. to obtain 1/2MS solid
Body culture medium；
1/4MS fluid medium: a great number of elements, trace element, iron salt and sugar composition are MS culture medium
1/4, Tris and MES regulation pH to 5.80 i.e. obtain 1/4MS fluid medium；
1/3MS fluid medium: a great number of elements, trace element, iron salt and sugar composition are that MS cultivates
The 1/3 of base, Tris and MES regulation pH to 5.80 i.e. obtains 1/3MS fluid medium.
2. the method for culturing seedlings of Herba Anoectochili roxburghii as claimed in claim 1, it is characterised in that will not have in step 2
The stem taken root and the step 3 camber seedling less than 2cm is had to join described solid matrix after smashing
In.
3. the method for culturing seedlings of Herba Anoectochili roxburghii as claimed in claim 2, it is characterised in that described solid matrix
Component including following parts by weight: 15 parts of soil, Vermiculitum 18 parts, 4 parts of humus, 15 parts of Fructus Musae,
Fructus Mangifera Indicae 1 part, peel of Carcinia mangostana L. 3 parts and 1 part of Hylocereus undatus peel.
4. the method for culturing seedlings of Herba Anoectochili roxburghii as claimed in claim 3, it is characterised in that by described Fructus Musae,
Fructus Mangifera Indicae, peel of Carcinia mangostana L. and Hylocereus undatus peel pulverize the granule being less than 40 mesh for particle diameter.
5. the method for culturing seedlings of Herba Anoectochili roxburghii as claimed in claim 1, it is characterised in that in described step 3,
Use plasma water preparation 1/4～1/3MS fluid medium.
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| CN104904600A (en) * | 2015-06-03 | 2015-09-16 | 吴华球 | Tissue-culturing and rapid-propagating method of anoectochilus formosanus |
| CN106134998B (en) * | 2016-07-13 | 2018-01-16 | 云南英纽生物科技有限公司 | A kind of tissue culture medium for anoectochilus roxburghii and its preparation method and application |
| CN106258957B (en) * | 2016-08-08 | 2018-05-25 | 四川千草生物技术股份有限公司 | A kind of roxburgh anoectochilus terminal bud is without hormone containerization cultural method |
| CN107432207A (en) * | 2017-08-24 | 2017-12-05 | 温光华 | A kind of roxburgh anoectochilus terminal bud transplants soil |
| CN107969339B (en) * | 2017-12-14 | 2019-12-31 | 泉州市金草生物技术有限公司 | Anoectochilus roxburghii cultivation method |
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