CN104082321B - A kind of bactericidal composition and preventing and treating the application in rice disease - Google Patents
A kind of bactericidal composition and preventing and treating the application in rice disease Download PDFInfo
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Abstract
The invention discloses a kind of bactericidal composition, this bactericidal composition contains pyraclostrobin and jinggangmycin A two kinds of active components, and the mass ratio of described pyraclostrobin and jinggangmycin A is 1: 1-16.The bactericidal action mechanism of two kinds of active components in the above-mentioned bactericidal composition of the present invention is different with the mode of action, experimental result shows, significant synergistic function can be produced after both are composite, successful is better than two-component single dose, effectively reduce the amount of application of each single dose, improve the safety to crop, have good effect to expanding fungicidal spectrum and delaying the Genes For Plant Tolerance property of medicine, extend the lasting period, there is significant promotional value.
Description
Technical field
The present invention relates to a kind of bactericidal composition and application thereof.
Background technology
False smut, rice blast, banded sclerotial blight are three kinds of Common Diseases of paddy rice, along with the popularization of the easily susceptible high-yield rice kind of tight grain type, Fertilization Level improve constantly continuing to increase of one-level planting density, these diseases have the trend increased the weight of year by year.Chemical pesticide control is current most important rice disease preventing measure, but a certain medicament of long-time single use will make germ develop immunity to drugs, and cause control efficiency to decline.By composite for the medicine of different mechanism of action, be the service life of prolong drug, reduce the most economical effective method of pesticide resistance of germ.
Pyraclostrobin (Pyraclostrobin) is the methoxy acrylic bactericide having pyrrazole structure concurrently, is a kind of mitochondrial respiratory inhibitor, plays drug effect by suppression spore germination and mycelial growth.Pyraclostrobin is except to except the direct effect of pathogen, the physiological change of many crops especially cereal can also be induced, as it can strengthen the absorption of activity raising to nitrogen of nitrate (nitrated) reductase, reduce the biosynthesis of ethene, delay crop aging, when crop is subject to disease invasion and attack, it can accelerate the generation of resisting albumen.Even when plant does not fall ill, pyraclostrobin also can by controlling secondary disease and alleviating the output improving crop from the pressure of abiotic component.
Jinggangmycin A (JingangmycinA) is water soluble antibiotics---the glucoside compounds produced by the mutation of water streptomycete well ridge, it is the agricultural antibiotic that systemic action is very strong, generation validoxylamine A can be hydrolyzed in bacterium spore, the activity of validoxylamine A energy Antifungi intracellular trehalose enzyme, and then affect the energy metabolism activity of germ, germ is hindered to transport glucose from mycelia base portion to mycelia top, the final growth suppressing mycelia.Jinggangmycin A is obviously greater than the direct effect of isolated condition to germ to the effect of germ on plant corpus, and can increase by successive induction plant defense response related enzyme activity.
Pyraclostrobin and jinggangmycin A are dissimilar bactericide, their bactericidal activity is different with the mechanism of action, likely can improve bactericidal activity after used in combination, realizes having complementary advantages, reduce consumption, thus become a kind of more economical, safe and effective bactericide.
Summary of the invention
For above-mentioned analysis, the object of this invention is to provide a kind of bactericidal composition, and provide said composition preventing and treating the application in rice disease.
Bactericidal composition of the present invention contains pyraclostrobin and jinggangmycin A two kinds of active components.
Preferably, the mass ratio of described pyraclostrobin and jinggangmycin A is 1: 1-16.
Best, the mass ratio of described pyraclostrobin and jinggangmycin A is 1: 2-4.
Bactericidal composition of the present invention can be used for preventing and treating rice green smut, rice blast, rice sheath blight disease.
The bactericidal action mechanism of two kinds of active components in the above-mentioned bactericidal composition of the present invention is different with the mode of action, experimental result shows, significant synergistic function can be produced after both are composite, successful is better than two-component single dose, effectively reduce the amount of application of each single dose, improve the safety to crop, have good effect to expanding fungicidal spectrum and delaying the Genes For Plant Tolerance property of medicine, extend the lasting period, there is significant promotional value.
Embodiment
Again foregoing of the present invention is described in further detail by the following examples, but this should be interpreted as the restriction to the above-mentioned subject area of the present invention.Without departing from the idea case in the present invention described above, the various replacement made according to ordinary skill knowledge and customary means or change, all should comprise within the scope of the invention.
Embodiment 1 pyraclostrobin and jinggangmycin A are to the co-toxicity of rice sheath blight disease and formulation screening test
1. materials and methods
1.1 for reagent product
The former medicine of 97.05% pyraclostrobin, the former medicine of 60% jinggangmycin A, provides by Wuhan Kenuo Biology Science & Technology Co., Ltd..Other reagent: dimethyl formamide, Tween 80.
1.2 strains tested
Rhizoctonia solani Kuhn (RhizoctoniasolaniK ü hn), plant protection Xi Zhibing teaching and research room of agricultural college of Agricultural University Of Jiangxi is separated cultivates.
1.3 other test equipments
SW-CJ-2FD type superclean bench, YSQ-G41-280 type hand-held pressure sterilizer, AR1140/C type electronic analytical balance, LRH-250-GS type climatic cabinate, 100ml volumetric flask, 9cm culture dish, conical flask, inoculator, card punch, slide measure, pipette etc.
1.4 test methods (Plating)
Cultivate 1.4.1 expand for examination bacterial classification
By Rhizoctonia solani Kuhn from PDA test tube slant media transfer to PDA plating medium, the constant incubator being placed in (28 ± 1) DEG C cultivates 3d, for subsequent use.
1.4.2 medicament configuration
Accurately take the former medicine 1.0304g of 97.05% pyraclostrobin with AR1140/C type electronic balance, dissolve with dimethyl formamide, load in 100ml volumetric flask, use dimethyl formamide constant volume, obtain 10g/L pyraclostrobin mother liquor; Accurately take the former medicine 1.6667g of 60% jinggangmycin A, dissolve with dimethyl formamide, load in 100ml volumetric flask, use dimethyl formamide constant volume, obtain 10g/L jinggangmycin A mother liquor; And then become following 5 kinds of formulas with two kinds of mother liquors by active ingredient mass fraction mixture:
Mixture A: pyraclostrobin: jinggangmycin A=1: 16;
Mixture B: pyraclostrobin: jinggangmycin A=1: 8;
Mixture C: pyraclostrobin: jinggangmycin A=1: 4;
Mixture D: pyraclostrobin: jinggangmycin A=1: 2;
Mixture E: pyraclostrobin: jinggangmycin A=1: 1.
On the basis of preliminary experiment, the mixture of different ratio in two single doses and above-mentioned 5 is diluted to 7 series concentration with the 0.1% Tween 80 aqueous solution by certain gradient (2 times of product moments) respectively for subsequent use.
1.4.3 medicament adds
On SW-CJ-2FD type superclean bench, according to test process, the medium melting sterilizing is quantitatively joined in aseptic conical flask, above-mentioned liquid is quantitatively drawn successively again from low concentration to high concentration, add respectively (every 90ml medium adds liquid 10ml) in above-mentioned conical flask, fully shake up.Then equivalent pours 5 diameters into is in the culture dish of 9cm, and the pastille making variable concentrations is dull and stereotyped.Separately establish and do not make blank (adding the 0.1% Tween 80 aqueous solution of equivalent) containing the process of medicament.
1.4.4 inoculation
The rice banded sclerotial blight cause of disease bacterium cultivating 3d is cut bacterium cake with the card punch of diameter 5cm from colony edge by superclean bench, with inoculator, pure culture biscuits involvng inoculation is dull and stereotyped central in pastille, mycelia faces up, 1, each culture dish side bacterium cake, each concentration repeats 5 times, and blank is equally by upper method process.The constant incubator postvaccinal culture dish being placed in (28 ± 1) DEG C is cultivated and is observed, and measures colony diameter after 2d.
1.4.5 result investigation and calculating
According to colony growth situation in blank culture dish, investigate each process mycelial growth inhibition rate, namely colony diameter is measured after cultivating 2d, with the colony diameter in vernier caliper measurement culture dish, each bacterium colony right-angled intersection is measured two data and is averaged, calculate mycelial growth inhibition rate, then according to log concentration---mycelial growth inhibition rate probit value analyzes (Bliss) method, obtains virulence regression equation and concentration (EC in suppressing with DPS2000 data processing software
50) value.Then according to the method that Sun Yunpei (Sun) introduces, the co-toxicity coefficient (CTC) of different blended ingredients is obtained.With following formulae discovery mycelial growth bacteriostasis rate:
2. results and analysis
The mixture of pyraclostrobin, jinggangmycin A and 5 kinds of different ratio is to virulence regression equation, the EC of Rhizoctonia solani Kuhn
50, EC
90measurement result refers to table 1.As known from Table 1, the mixture of 5 kinds of different blended ratio all has higher synergistic effect to Rhizoctonia solani Kuhn, and mixture A, B, C, D, E co-toxicity coefficient is respectively 151.15,158.49,163.35,161.35 and 137.64.Wherein again with pyraclostrobin: jinggangmycin A=1: the proportioning synergistic effect of 4 and 1: 2 is relatively obvious, and co-toxicity coefficient is respectively 163.27 and 161.35.
Table 1 pyraclostrobin, jinggangmycin A and 5 kinds of mixtures are to the Toxicity Determination result of Rhizoctonia solani Kuhn
3. brief summary
Test determination result shows, pyraclostrobin and jinggangmycin A mixture, the mixture of 5 kinds of different ratio all has good synergistic effect to Rhizoctonia solani Kuhn, wherein again with pyraclostrobin: jinggangmycin A=1: the proportioning synergistic effect of 2 and 1: 4 is relatively obvious.According to the physicochemical property of two kinds of medicaments and applicable cases aborning, pyraclostrobin and jinggangmycin A can be become the preparation of 30% by 1 to 2 or 1 to 4 mixture, i.e. pyraclostrobin-containing 10% in mixture, containing jinggangmycin A 20%, or pyraclostrobin-containing 6%, containing jinggangmycin A 24%.
Embodiment 2 pyraclostrobin and jinggangmycin A are to the co-toxicity of false smut and formulation screening test
1. materials and methods
1.1 for reagent product
The former medicine of 97.05% pyraclostrobin, the former medicine of 60% jinggangmycin A, provides by Wuhan Kenuo Biology Science & Technology Co., Ltd..Other reagent: dimethyl formamide, Tween 80.
1.2 strains tested
False smut germ (Ustilaginoideavirens (Cooke) Takahashi), plant protection Xi Zhibing teaching and research room of agricultural college of Agricultural University Of Jiangxi is separated cultivates.
1.3 other test equipments
SW-CJ-2FD type superclean bench, YSQ-G41-280 type hand-held pressure sterilizer, AR1140/C type electronic analytical balance, LRH-250-GS type climatic cabinate, micro-adjustable pipette, 100ml volumetric flask, 9cm culture dish, conical flask, inoculator, card punch, slide measure, pipette etc.
1.4 test methods (Plating)
Cultivate 1.4.1 expand for examination bacterial classification
By rice false smut from PDA test tube slant media transfer to PDA plating medium, the constant incubator being placed in (28 ± 1) DEG C cultivates 20d, for subsequent use.
1.4.2 medicament configuration
Accurately take the former medicine 1.0304g of 97.05% pyraclostrobin with AR1140/C type electronic balance, dissolve with dimethyl formamide, load in 100ml volumetric flask, use dimethyl formamide constant volume, obtain 10g/L pyraclostrobin mother liquor; Accurately take the former medicine 1.6667g of 60% jinggangmycin A, dissolve with dimethyl formamide, load in 100ml volumetric flask, use dimethyl formamide constant volume, obtain 10g/L jinggangmycin A mother liquor; And then become following 5 kinds of formulas with two kinds of mother liquors by active ingredient mass fraction mixture:
Mixture A: pyraclostrobin: jinggangmycin A=1: 16;
Mixture B: pyraclostrobin: jinggangmycin A=1: 8;
Mixture C: pyraclostrobin: jinggangmycin A=1: 4;
Mixture D: pyraclostrobin: jinggangmycin A=1: 2;
Mixture E: pyraclostrobin: jinggangmycin A=1: 1.
On the basis of preliminary experiment, the mixture of different ratio in two single doses and above-mentioned 5 is diluted to 7 series concentration with the 0.1% Tween 80 aqueous solution by certain gradient (2 times of product moments) respectively for subsequent use.
1.4.3 medicament adds
On SW-CJ-2FD type superclean bench, according to test process, the medium melting sterilizing is quantitatively joined in aseptic conical flask, above-mentioned liquid is quantitatively drawn successively again from low concentration to high concentration, add respectively (every 90ml medium adds liquid 10ml) in above-mentioned conical flask, fully shake up.Then equivalent pours 5 diameters into is in the culture dish of 9cm, and the pastille making variable concentrations is dull and stereotyped.Separately establish and do not make blank (adding the 0.1% Tween 80 aqueous solution of equivalent) containing the process of medicament.
1.4.4 inoculation
The former bacterium of false smut cultivating 20d is cut bacterium cake with the card punch of diameter 5cm from colony edge by superclean bench, with inoculator, pure culture biscuits involvng inoculation is dull and stereotyped central in pastille, mycelia faces up, and each culture dish puts 1 bacterium cake, each concentration repeats 5 times, and blank is equally by upper method process.The constant incubator postvaccinal culture dish being placed in (28 ± 1) DEG C is cultivated and is observed, and measures colony diameter after 14d.
1.4.5 result investigation and calculating
According to colony growth situation in blank culture dish, investigate each process mycelial growth inhibition rate, namely colony diameter is measured after cultivating 14d, with the colony diameter in vernier caliper measurement culture dish, each bacterium colony right-angled intersection is measured two data and is averaged, calculate mycelial growth inhibition rate, then according to log concentration---mycelial growth inhibition rate probit value analyzes (Bliss) method, obtains virulence regression equation and concentration (EC in suppressing with DPS2000 data processing software
50) value.Then according to the method that Sun Yunpei (Sun) introduces, the co-toxicity coefficient (CTC) of different blended ingredients is obtained.With following formulae discovery mycelial growth bacteriostasis rate:
2. results and analysis
The mixture of pyraclostrobin, jinggangmycin A and 5 kinds of different ratio is to virulence regression equation, the EC of rice false smut
50, EC
90measurement result refers to table 2.As known from Table 2, the mixture of 5 kinds of different blended ratio all has higher synergistic effect to Rhizoctonia solani Kuhn, and mixture A, B, C, D, E co-toxicity coefficient is respectively 138.25,146.66,156.62,158.87 and 141.71.Wherein again with pyraclostrobin: jinggangmycin A=1: the proportioning synergistic effect of 4 and 1: 2 is relatively obvious, and co-toxicity coefficient is respectively 156.62 and 158.87.
Table 2 pyraclostrobin, jinggangmycin A and 5 kinds of mixtures are to the Toxicity Determination result of rice false smut
3. brief summary
Test determination result shows, pyraclostrobin and jinggangmycin A mixture, and the mixture of 5 kinds of different ratio all has good synergistic effect to rice false smut, wherein again with pyraclostrobin: jinggangmycin A=1: the proportioning synergistic effect of 2 and 1: 4 is relatively obvious.According to the physicochemical property of two kinds of medicaments and applicable cases aborning, pyraclostrobin and jinggangmycin A can be become the preparation of 30% by 1 to 2 or 1 to 4 mixture, i.e. pyraclostrobin-containing 10% in mixture, containing jinggangmycin A 20%, or pyraclostrobin-containing 6%, containing jinggangmycin A 24%.
Embodiment 3 pyraclostrobin and jinggangmycin A are to the co-toxicity of rice blast and formulation screening test
1. materials and methods
1.1 for reagent product
The former medicine of 97.05% pyraclostrobin, the former medicine of 60% jinggangmycin A, provides by Wuhan Kenuo Biology Science & Technology Co., Ltd..Other reagent: dimethyl formamide, Tween 80.
1.2 strains tested
Rice blast pathogen PiriculariaoryzaeCav., PiriculariagriseaSacc., plant protection Xi Zhibing teaching and research room of agricultural college of Agricultural University Of Jiangxi is separated cultivates.
1.3 other test equipments
3WPSH-500E type is raw surveys spray tower, SW-CJ-2FD type superclean bench, 100m
2the plastic basin alms bowl, beaker, micro-adjustable pipette, culture dish, volumetric flask, conical flask, graduated cylinder, inoculator etc. of greenhouse by solar heat, YSQ-G41-280 type hand-held pressure sterilizer, AR1140/C type electronic analytical balance, LRH-250-GS type climatic cabinate, YN-2D-10 type stainless steel electrical distiller, length × wide=25cm × 16cm.
1.4 test methods (pot-culture method)
1.4.1 rice seedling prepares
Taken outdoors soil (content of organic matter approximates about 1.8%, PH6.8) dries, shatters, sieves.After dress soil, native surface area is about 400cm
2plastic basin alms bowl in load quantitative (4/5 place of basin alms bowl) sieved soil, slightly suppress.From the pouring of basin alms bowl top, soil completely moistening extremely soil table is made to have a small amount of ponding.Will for examination rice paddy seed (susceptible pinkish red Suyunuo) vernalization to showing money or valuables one carries unintentionally, selecting the consistent seed that germinates broadcasts in basin alms bowl, every basin evenly sows 20, steps on paddy, after planting moves into cellar culture in greenhouse by solar heat, when rice seedling grows to 2-3 leaf, strain is determined in thinning, and every basin retains basically identical rice seedling 10 strain of growth, and ensures that the density of rice seedling is basically identical as far as possible, continue to be cultured to for 3 leaf 1 heart stages, then carry out cauline leaf spraying process.
1.4.2 the preparation of magnaporthe grisea spore suspension
After adding 1000ml water boil 15min with 40g straw, get straw juice and add 30g corn flour and 20g agar again, be settled to 1000ml.Packing, as after 2 Boiling tube sterilizings, medium slant is inoculated rice blast hypha block, cultivates 7d at 25 DEG C, then cultivates 7d under being placed in black light lamp, can produce a large amount of spore.With spore under aseptic washing, elimination mycelia, configures into about 1 × 10
5individual/ml to 1 × 10
7individual/ml spore suspension, for subsequent use.
1.4.3 medicament configuration
Accurately take the former medicine 1.0304g of 97.05% pyraclostrobin with AR1140/C type electronic balance, dissolve with dimethyl formamide, load in 100ml volumetric flask, use dimethyl formamide constant volume, obtain 10g/L pyraclostrobin mother liquor; Accurately take the former medicine 1.6667g of 60% jinggangmycin A, dissolve with dimethyl formamide, load in 100ml volumetric flask, use dimethyl formamide constant volume, obtain 10g/L jinggangmycin A mother liquor; And then become following 5 kinds of formulas with two kinds of mother liquors by active ingredient mass fraction mixture:
Mixture A: pyraclostrobin: jinggangmycin A=1: 16;
Mixture B: pyraclostrobin: jinggangmycin A=1: 8;
Mixture C: pyraclostrobin: jinggangmycin A=1: 4;
Mixture D: pyraclostrobin: jinggangmycin A=1: 2;
Mixture E: pyraclostrobin: jinggangmycin A=1: 1.
On the basis of preliminary experiment, the mixture of different ratio in two single doses and above-mentioned 5 is diluted to 7 series concentration with the 0.1% Tween 80 aqueous solution by certain gradient (2 times of product moments) respectively for subsequent use.
1.4.4 chemicals treatment
Survey raw for the 3 leaf 1 heart stage rice seedling 3WPSH-500E types cultivated the liquid that spray towers spray above-mentioned series concentration, spraying liquid amount (3ml/ basin) is covered with till medicine drips with blade, and liquid sedimentation 1min is for subsequent use after liquid natural air drying.Often process 1 basin, repeat for 4 times, separately establish the process only containing solvent and surfactant not containing active ingredient to make blank.
1.4.5 inoculation and cultivation
24h inoculation after chemicals treatment, survey spray tower spray inoculation with 3WPSH-500E type is raw, every ware meets above-mentioned spore suspension 1ml, liquid sedimentation 1min.Move into greenhouse by solar heat cellar culture after inoculation, improve the humidity in greenhouse as far as possible, with the moisturizing of basin alms bowl top pouring mode, by the temp. and humidity in wet-and-dry-bulb thermometer periodic logging greenhouse.
1.4.6 result investigation and calculating
When the sick leaf rate of blank reaches more than 50%, the incidence of classification investigation whole rice blade, stage division is as follows:
0 grade: whole strain is anosis;
1 grade: occur brown scab;
3 grades: occur typical spindle scab, lesion area accounts for less than 5% of whole leaf area;
5 grades: typical scab, lesion area accounts for the 6-25% of whole leaf area;
7 grades: typical scab, lesion area accounts for the 26-50% of whole leaf area;
9 grades: typical scab, lesion area accounts for more than 50% of whole leaf area.
Calculate disease index and the control efficiency (disease refers to inhibiting rate) of each process according to this, again according to log concentration---disease refers to that inhibiting rate probit value analyzes (Bliss) method, with DPS data processing software calculate virulence regression equation and disease refer to suppress in concentration (EC
50) value.Then according to the method that Sun Yunpei (Sun) introduces, the co-toxicity coefficient (CTC) of each mixed agent is obtained.Disease index and disease refer to that inhibiting rate computing formula is as follows:
2. results and analysis
The mixture of pyraclostrobin, jinggangmycin A and 5 kinds of different ratio is to virulence regression equation, the EC of rice blast
50, EC
90measurement result refers to table 3.As known from Table 3, the mixture of 5 kinds of different blended ratio all has good synergistic effect to rice blast, and mixture A, B, C, D, E co-toxicity coefficient is respectively 140.26,145.25,153.08,159.82 and 139.86.Wherein again with pyraclostrobin: jinggangmycin A=1: the proportioning synergistic effect of 2 is relatively obvious, and co-toxicity coefficient reaches 159.82.
Table 3 pyraclostrobin, jinggangmycin A and 5 kinds of mixtures are to the Toxicity Determination result of rice blast
3. brief summary
Test determination result shows, pyraclostrobin and jinggangmycin A mixture, and the mixture of 5 kinds of different ratio all has good synergistic effect to rice blast, wherein again with pyraclostrobin: jinggangmycin A=1: the proportioning synergistic effect of 2 is relatively obvious.According to the physicochemical property of two kinds of medicaments and applicable cases aborning, pyraclostrobin and jinggangmycin A can be become the preparation of 30% by 1 to 2 mixture, i.e. pyraclostrobin-containing 10% in mixture, containing jinggangmycin A 20%.
Claims (1)
1. the application of bactericidal composition in control rice green smut, described bactericidal composition contains pyraclostrobin and jinggangmycin A two kinds of active components, and the mass ratio of described pyraclostrobin and jinggangmycin A is 1: 2-4.
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| CN103237451A (en) * | 2010-12-08 | 2013-08-07 | 巴斯夫欧洲公司 | Fungicidal mixtures |
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| CN103237451A (en) * | 2010-12-08 | 2013-08-07 | 巴斯夫欧洲公司 | Fungicidal mixtures |
| CN102302026A (en) * | 2011-03-12 | 2012-01-04 | 陕西汤普森生物科技有限公司 | Bactericidal composition containing pyraclostrobin and antibiotic compounds |
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