CN104054504B - The breeding method of bee pupa Chinese caterpillar fungus - Google Patents
The breeding method of bee pupa Chinese caterpillar fungus Download PDFInfo
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Abstract
The invention discloses the breeding method of a kind of bee pupa Chinese caterpillar fungus, taking bee variety worm as material, with honeycomb spleen material as cultivation matrix, guarantee high-quality and the high-load of functional component in bee pupa worm grass product, adopt sterilization treatment honeycomb simulation bee pupa Chinese caterpillar fungus natural ecological environment, artificial culture forms, success ratio of inoculation, postvaccinal rigid rate is high, bee pupa Chinese caterpillar fungus contains a large amount of cordycepins, cordycepic acid, adenosine, worm polysaccharide, protein, SOD isoreactivity composition, can strengthen immunity, anti-knurl gives protection against cancer, anti-aging, invigorate the lung and the kidney etc., it is human nutrition nourishing, the dietotherapy that the benefit of prolonging life lengthens one's life, the rare good merchantable brand of herbal cuisine.
Description
Technical field
The present invention relates to the breeding method of a kind of bee pupa Chinese caterpillar fungus.
Background technology
Natural cs is rare, expensive, adopts tame method, utilizes mycelium to carry out submerged fermentationThe Cordyceps militaris of turning out, drug effect is basic identical with Cordyceps sinensis, swarms so be beneficial to silkworm chrysalis cultivation Cordyceps militaris familyAnd rise, cultivation technique condition is uneven, occurs focusing on high yield in production, ignores showing of drug effect health careResemble, industry price competition fierceness, these bad behaviors have affected Chinese caterpillar fungus service quality, and price glides, seriousThe sound development of restriction China Cordyceps militaris industry.
For breaking through the bottleneck that suppresses the development of pupa worm industry, we select the honeybee pupa worm with high-quality functional component to beMaterial, for the functional component that improves Cordyceps militaris lays the foundation; Select on the other hand honeycomb spleen material to increase functionThe cultivation matrix of composition, guarantees high-quality and the high-load of functional component in bee pupa worm grass product.
Bee pupa has another name called honeybee embryo, honeybee son. Being described as the treasure in food, is to contain abundant high protein, lowThe desirable green nourishing food of fat, a small amount of lecithin, multivitamin and trace element. Without appointingWhat processing, is not polluted. Bee pupa both can be used as food, can be used as again nutrient and healthcare products. Bee pupa is containing eggWhite matter 20.3%, lipase 37 .5%, carbohydrate 19.5%, trace element 0.5%, moisture 42.7%.The nutritive value of bee pupa is not less than pollen, and especially the content of vitamin A substantially exceeds beef, protein withDried fish wet goods exceedes 10 times of dried fish oil with, vitamin D, containing the patron saint of human health " 21 century "Germanium, selenium etc., its contained active material is that human body cannot produce voluntarily, is described as " ginseng in the sky ", "The refreshing worm of secret ". Have, brain tonic and intelligence development, the cytothesis that strengthens memory, urgees the organs such as liver kidney, nerve,Body is upgraded, thus can benefiting qi and nourishing blood, the strong waist of strong kidney, the ease constipation of a surname's lung.
Honeycomb be honeybee store honey, slurry, powder, glue, wax, etc. material and the life area that raises up seed, respectivelyState expert analyzes and researches and finds that honeycomb contains beeswax, resin, grease, pigment, tannin, carbohydrate, hasThe compositions such as machine acid, aliphatic acid and glucoside, a large amount of trace elements iron, calcium, copper, potassium, cobalt etc. EspeciallyIts amino acid content is very abundant reaches 22.6%, for 3.97 times of honey 5.7% and contain pharmacological actionVery strong taurine, average content reaches every gram of 0.154mg, be about every gram of honey containing (0.044mg) 3.5Doubly. In B.C. Shennong's Herbal, just have that " beeswax (honeycomb) taste is sweet, tepor, nontoxic masterControl the inferior the dense blood of disease, bowl spares, beneficial gas " note plant, application honeycomb treatment rhinitis and blister sore, fatty liver also have veryLong history, modern study shows that honeycomb has the effects such as antibacterial, anti-inflammatory, inhibition tumour. China is fosterHoneybee big country, bee product aboundresources, the honey of most of honeybee, powder, slurry, glue, wax, pupa, all obtainedWell development utilization, only has honeycomb only main as extracting the beeswax that wherein content is 25% only,And other material that contains a large amount of nutrition and medicinal health does not well develop, cause the wave of resourceTake the pollution with environment.
Bee pupa Chinese caterpillar fungus contains a large amount of cordycepins, cordycepic acid, adenosine, Cordyceps sinensis polysaccharide, protein, SOD etc. and livesSexual element, can strengthen immunity, the anti-knurl that gives protection against cancer, anti-aging, invigorate the lung and the kidney etc., be human nutrition nourishing,The rare good merchantable brand of dietotherapy, herbal cuisine that the benefit of prolonging life lengthens one's life.
Summary of the invention
The present invention provides the breeding method of a kind of bee pupa Chinese caterpillar fungus in order to solve deficiency of the prior art, selectThe honeybee pupa worm with high-quality functional component is material, for the functional component that improves Cordyceps militaris lays the foundation; SeparatelySelected on the one hand honeycomb spleen material increase functional component cultivation matrix, guarantee function in bee pupa worm grass productThe high-quality of composition and content.
For achieving the above object, the technical solution used in the present invention is:
A breeding method for bee pupa Chinese caterpillar fungus, comprises the following steps
(1) screening of bee pupa Cordyceps Militaris bacterial strain, by the method for Cordyceps strain spore application spore separationObtain the pure bacterial strain of Cordyceps sinensis;
(2) the organic culture medium of bee pupa is made: adopt live body bee pupa or peeling bee pupa worm live body protein 10 0g,Large thin rice gruel 200g, camellia powder 10mg, potassium dihydrogen phosphate 2g, put into the beaker of 1000mL, add water 25～45mL to add thermal agitation miscible, completes after miscible and stops heating, and packing test tube is jumped a queue cooling, makesThe organic culture medium of bee pupa of semi-liquid-like;
(3) making of spore suspension: the bee pupa Chinese caterpillar fungus strain that (1) step is cultivated is inoculated in (2)On the organic culture medium of bee pupa that step is made, put at 20 DEG C and cultivate, after there is conidium, picking part is placed onIn sterilized water, make spore suspension stand-by;
(4) host prepares: live body bee pupa is carried out disinfection and makes host with thimerosal;
(5) infection of bee pupa Chinese caterpillar fungus: dip in spore suspension at transfer needle needle point and thrust in above-mentioned bee pupa body,Only inject by 0.15～0.25mg/;
(6) protection of infection bee pupa body: the bee pupa body after microbiological contamination is divided in bamboo weaving basket, protected under room temperatureProtect to pupal cell stiff;
(7) cultivation of stroma: the natural ecological environment of simulation bee pupa Chinese caterpillar fungus, cultivate stiff pupal cell and growStroma, method is with the loose nest slag after honeycomb Extraction parts nutriment, after sterilization treatment stiffOn pupal cell, cover one deck, loose ventilative being placed at 15～30 DEG C of temperature, relative air humidity is 80～95%Natural lighting environmental condition cultivate;
(8) Collection and conservation of bee pupa Chinese caterpillar fungus: after stroma maturation, dig out cleaning, carry out body with edible alcoholTable sterilization, then be placed under 60 DEG C of environment and dry 2～4 hours, put shady and cool dry place sealing and preserve.
As a kind of preferred version of the breeding method of bee pupa Chinese caterpillar fungus of the present invention, described thimerosal isOne in 75% alcohol or amino acid iodine.
As a kind of preferred version of the breeding method of bee pupa Chinese caterpillar fungus of the present invention, the training of described stromaEducate for by stiff pupa head upwards or pupal cell is equidirectional keeps flat, insert or put into treated honeycomb slag and add weedtreeBits mix on the porous nest bedstead doing, and are placed at 15～30 DEG C of temperature, and relative air humidity is 80～95%Natural lighting environmental condition cultivate.
The breeding method that the present invention discloses a kind of bee pupa Chinese caterpillar fungus, comprises the following steps:
(1) screening of bee pupa Cordyceps Militaris bacterial strain, by the method for Cordyceps strain spore application spore separationObtain the pure bacterial strain of Cordyceps sinensis;
(2) the organic culture medium of bee pupa is made: adopt live body bee pupa or peeling bee pupa worm live body protein 10 0g,Large thin rice gruel 200g, camellia powder 10mg, potassium dihydrogen phosphate 2g, put into the beaker of 1000mL, add water 25～45mL to add thermal agitation miscible, completes after miscible and stops heating, and packing test tube is jumped a queue cooling, makesThe organic culture medium of bee pupa of semi-liquid-like;
(3) making of spore suspension: the bee pupa Chinese caterpillar fungus strain that (1) step is cultivated is inoculated in (2)On the organic culture medium of bee pupa that step is made, put at 20 DEG C and cultivate, after there is conidium, picking part is placed onIn sterilized water, make spore suspension stand-by;
(4) host prepares: dry bee pupa is pulverized and made bee pupa powder and honeycomb Extraction parts nutrientsLoose nest slag after matter is mixed and made into culture medium, and the spore suspension of making in step (3) is seeded in to trainingSupport in base, be placed at 15～30 DEG C of temperature the natural lighting environment bar that relative air humidity is 80～95%Part is cultivated;
(5) Collection and conservation of bee pupa Chinese caterpillar fungus: after stroma maturation, pick, be placed under 60 DEG C of environment and dryDry 2～4 hours, put shady and cool dry place sealing and preserve.
Beneficial effect:
The breeding method of bee pupa Chinese caterpillar fungus of the present invention selects the honeybee pupa worm with high-quality functional component to beMaterial, improves the functional component of Cordyceps militaris greatly; Select the cultivation matrix of honeycomb spleen material increase functional component,Guarantee high-quality and the high-load of functional component in bee pupa worm grass product; In step (7), honeycomb extractsThe complementary basis slag charge of nutriment and this high nutrition can further be developed reasonable application, and carrying out special process canTo produce high-grade bee pupa cordyceps militaris liquor, senior drink, nourishing-paste, food etc.; Residue can be used as animal feed againWith some industrial chains such as vegetable fertilizer, turn waste into wealth, be that apiculture accelerates to increase economic benefit, develop in a healthy way,Promotion agricultural production and income is made contributions.
Detailed description of the invention
Embodiment 1
The breeding method of bee pupa Chinese caterpillar fungus:
(1) screening of bee pupa Cordyceps Militaris bacterial strain, by the method for Cordyceps strain spore application spore separationObtain the pure bacterial strain of Cordyceps sinensis;
(2) the organic culture medium of bee pupa is made: adopt the live body protein 10 0g of live body bee pupa or peeling bee pupa worm,Large thin rice gruel 200g, camellia powder 10mg, potassium dihydrogen phosphate 2g, puts into the beaker of 1000mL, adds water 25And add thermal agitation miscible, complete after miscible and stop heating, that packing test tube is jumped a queue is cooling, make semi-liquid-likeThe organic culture medium of bee pupa;
(3) making of spore suspension: the bee pupa Chinese caterpillar fungus strain that (1) step is cultivated is inoculated in (2)On the organic culture medium of bee pupa that step is made, put at 20 DEG C and cultivate, after there is conidium, picking part is placed onIn sterilized water, make spore suspension stand-by;
(4) host prepares: by the 75% alcohol 6-8 minute that carries out disinfection for live body bee pupa;
(5) infection of bee pupa Chinese caterpillar fungus: dip in spore suspension at transfer needle needle point and thrust in above-mentioned bee pupa body,Only inject by 0.15mg/;
(6) protection of infection bee pupa body: the bee pupa body after microbiological contamination is divided in bamboo weaving basket, protected under room temperatureProtect to pupal cell stiff;
(7) cultivation of stroma: the natural ecological environment of simulation bee pupa Chinese caterpillar fungus, cultivate stiff pupal cell and growStroma, method is by stiff pupa head upwards or pupal cell is equidirectional keeps flat, and inserts or put into treated honeycombSlag adds weed tree sawdust and mixes on the porous nest bedstead doing, and is placed at 15～30 DEG C of temperature, and relative air humidity is80～95% natural lighting environmental condition is cultivated;
(8) Collection and conservation of bee pupa Chinese caterpillar fungus: after stroma maturation, dig out cleaning, carry out body with edible alcoholTable sterilization, then put under 60 DEG C of environment and dry 2 hours, put shady and cool dry place sealing and preserve.
Embodiment 2
The breeding method of bee pupa Chinese caterpillar fungus:
(1) screening of bee pupa Cordyceps Militaris bacterial strain, by the method for Cordyceps strain spore application spore separationObtain the pure bacterial strain of Cordyceps sinensis;
(2) the organic culture medium of bee pupa is made: adopt the live body protein 10 0g of live body bee pupa or peeling bee pupa worm,Large thin rice gruel 200g, camellia powder 10mg, potassium dihydrogen phosphate 2g, puts into the beaker of 1000mL, and 35mL adds waterAnd add thermal agitation miscible, complete after miscible and stop heating, that packing test tube is jumped a queue is cooling, make semi-liquid-likeThe organic culture medium of bee pupa;
(3) making of spore suspension: the bee pupa Chinese caterpillar fungus strain that (1) step is cultivated is inoculated in (2)On the organic culture medium of bee pupa that step is made, put at 20 DEG C and cultivate, after there is conidium, picking part is placed onIn sterilized water, make spore suspension stand-by;
(4) host prepares: by the 75% alcohol 6-8 minute that carries out disinfection for live body bee pupa;
(5) infection of bee pupa Chinese caterpillar fungus: dip in spore suspension at transfer needle needle point and thrust in above-mentioned bee pupa body,Only inject by 0.2mg/;
(6) protection of infection bee pupa body: the bee pupa body after microbiological contamination is divided in bamboo weaving basket, protected under room temperatureProtect to pupal cell stiff;
(7) cultivation of stroma: the natural ecological environment of simulation bee pupa Chinese caterpillar fungus, cultivate stiff pupal cell and growStroma, method is with the loose nest slag after honeycomb Extraction parts nutriment, after sterilization treatment stiffOn pupal cell, cover one deck, loose ventilative being placed at 20 DEG C of temperature, the nature that relative air humidity is 85%Photoenvironment condition is cultivated;
(8) Collection and conservation of bee pupa Chinese caterpillar fungus: after stroma maturation, dig out cleaning, carry out body with edible alcoholTable sterilization, then put under 60 DEG C of environment and dry 2 hours, put shady and cool dry place sealing and preserve.
Embodiment 3
The breeding method of bee pupa Chinese caterpillar fungus:
(1) screening of bee pupa Cordyceps Militaris bacterial strain, by the method for Cordyceps strain spore application spore separationObtain the pure bacterial strain of Cordyceps sinensis;
(2) the organic culture medium of bee pupa is made: adopt the live body protein 10 0g of live body bee pupa or peeling bee pupa worm,Large thin rice gruel 200g, camellia powder 10mg, potassium dihydrogen phosphate 2g, puts into the beaker of 1000mL, and 45mL adds waterAnd add thermal agitation miscible, complete after miscible and stop heating, that packing test tube is jumped a queue is cooling, make semi-liquid-likeThe organic culture medium of bee pupa;
(3) making of spore suspension: the bee pupa Chinese caterpillar fungus strain that (1) step is cultivated is inoculated in (2)On the organic culture medium of bee pupa that step is made, put at 20 DEG C and cultivate, after there is conidium, picking part is placed onIn sterilized water, make spore suspension stand-by;
(4) host prepares: by amino acid iodine sterilization 1 minute for live body bee pupa;
(5) infection of bee pupa Chinese caterpillar fungus: dip in spore suspension at transfer needle needle point and thrust in above-mentioned bee pupa body,Only inject by 0.5mg/;
(6) protection of infection bee pupa body: the bee pupa body after microbiological contamination is divided in bamboo weaving basket, protected under room temperatureProtect to pupal cell stiff;
(7) cultivation of stroma: the natural ecological environment of simulation bee pupa Chinese caterpillar fungus, cultivate stiff pupal cell and growStroma, method is with the loose nest slag after honeycomb Extraction parts nutriment, after sterilization treatment stiffOn pupal cell, cover one deck, loose ventilative being placed at 30 DEG C of temperature, the nature that relative air humidity is 95%Photoenvironment condition is cultivated;
(8) Collection and conservation of bee pupa Chinese caterpillar fungus: after stroma maturation, dig out cleaning, carry out body with edible alcoholTable sterilization, then put under 60 DEG C of environment and dry 4 hours, put shady and cool dry place sealing and preserve.
Embodiment 4
The breeding method of bee pupa Chinese caterpillar fungus:
(1) screening of bee pupa Cordyceps Militaris bacterial strain, by the method for Cordyceps strain spore application spore separationObtain the pure bacterial strain of Cordyceps sinensis;
(2) the organic culture medium of bee pupa is made: adopt live body bee pupa or peeling bee pupa worm live body protein 10 0g,Large thin rice gruel 200g, camellia powder 10mg, potassium dihydrogen phosphate 2g, put into the beaker of 1000mL, add water 25～45mL to add thermal agitation miscible, completes after miscible and stops heating, and packing test tube is jumped a queue cooling, makesThe organic culture medium of bee pupa of semi-liquid-like;
(3) making of spore suspension: the bee pupa Chinese caterpillar fungus strain that (1) step is cultivated is inoculated in (2)On the organic culture medium of bee pupa that step is made, put at 20 DEG C and cultivate, after there is conidium, picking part is placed onIn sterilized water, make spore suspension stand-by;
(4) host prepares: dry bee pupa is pulverized and made bee pupa powder and honeycomb Extraction parts nutrientsLoose nest slag after matter is mixed culture bed, and the spore suspension of making in step (3) is seeded in to trainingSupport in base, be placed at 20 DEG C of temperature, the natural lighting environmental condition that relative air humidity is 90% is cultivated;
(5) Collection and conservation of bee pupa Chinese caterpillar fungus: after stroma maturation, pick, be placed under 60 DEG C of environment and dryDry 2 hours, put shady and cool dry place sealing and preserve.
Embodiment 5
The breeding method of bee pupa Chinese caterpillar fungus:
(1) screening of bee pupa Cordyceps Militaris bacterial strain, by the method for Cordyceps strain spore application spore separationObtain the pure bacterial strain of Cordyceps sinensis;
(2) the organic culture medium of bee pupa is made: adopt live body bee pupa or peeling bee pupa worm live body protein 10 0g,Large thin rice gruel 200g, camellia powder 10mg, potassium dihydrogen phosphate 2g, put into the beaker of 1000mL, add water 25～45mL to add thermal agitation miscible, completes after miscible and stops heating, and packing test tube is jumped a queue cooling, makesThe organic culture medium of bee pupa of semi-liquid-like;
(3) making of spore suspension: the bee pupa Chinese caterpillar fungus strain that (1) step is cultivated is inoculated in (2)On the organic culture medium of bee pupa that step is made, put at 20 DEG C and cultivate, after there is conidium, picking part is placed onIn sterilized water, make spore suspension stand-by;
(4) host prepares: dry bee pupa is pulverized and made bee pupa powder and honeycomb Extraction parts nutrientsLoose nest slag after matter is mixed and made into culture medium, and the spore suspension of making in step (3) is seeded in to trainingSupport in base, be placed at 30 DEG C of temperature, the natural lighting environmental condition that relative air humidity is 90% is cultivated;
(5) Collection and conservation of bee pupa Chinese caterpillar fungus: after stroma maturation, pick, be placed under 60 DEG C of environment and dryDry 4 hours, put shady and cool dry place sealing and preserve.
Although embodiments of the present invention are illustrated in description, these embodiments are conductPrompting, should not limit protection scope of the present invention. Carry out without departing from the spirit and scope of the present invention various provincesSlightly, displacement and change all should be included in protection scope of the present invention.
Claims (4)
1. a breeding method for bee pupa Chinese caterpillar fungus, is characterized in that, comprises the following steps
(1) screening of bee pupa Cordyceps Militaris bacterial strain, obtains the winter by the method for Cordyceps strain spore application spore separationThe pure bacterial strain of worm summer grass;
(2) the organic culture medium of bee pupa is made: the live body protein 10 0g, the rice that adopt live body bee pupa or peeling bee pupa wormSoup 200g, camellia powder 10mg, potassium dihydrogen phosphate 2g, put into the beaker of 1000mL, add water 25～45mL to add thermal agitation miscible, completes after miscible and stops heating, and packing test tube is jumped a queue cooling,Make the organic culture medium of bee pupa of semi-liquid-like;
(3) making of spore suspension: the bee pupa Chinese caterpillar fungus strain that (1) step is cultivated is inoculated in (2) step systemOn the organic culture medium of bee pupa of doing, put at 20 DEG C and cultivate, after there is conidium, picking part is placed onIn sterilized water, make spore suspension stand-by;
(4) host prepares: live body bee pupa is carried out disinfection and makes host with thimerosal;
(5) infection of bee pupa Chinese caterpillar fungus: dip in spore suspension at transfer needle needle point and thrust in above-mentioned bee pupa body, by 0.15～0.25mg/ only injects;
(6) protection of infection bee pupa body: the bee pupa body after microbiological contamination is divided in bamboo weaving basket, protected to pupa under room temperatureBody is stiff;
(7) cultivation of stroma: the natural ecological environment of simulation bee pupa Chinese caterpillar fungus, cultivate stiff pupal cell and grow stroma,Method is with the loose nest slag after honeycomb Extraction parts nutriment, after sterilization treatment at stiff pupaOn body, covering one deck, loose ventilative being placed at 15～30 DEG C of temperature, relative air humidity is 80～95% natural lighting environmental condition is cultivated;
(8) Collection and conservation of bee pupa Chinese caterpillar fungus: after stroma maturation, dig out cleaning, carry out body surface sterilization with edible alcohol,Be placed on again under 60 DEG C of environment and dry 2～4 hours, put shady and cool dry place sealing and preserve.
2. the breeding method of bee pupa Chinese caterpillar fungus according to claim 1, is characterized in that: described step (4)In thimerosal be the one in 75% alcohol or amino acid iodine.
3. the breeding method of bee pupa Chinese caterpillar fungus according to claim 1, is characterized in that: described step (7)The cultivation of middle stroma is by stiff pupa head upwards or pupal cell is equidirectional keeps flat, and inserts or put into treated honeybeeNest slag adds weed tree sawdust and mixes on the porous nest bedstead doing, and is placed at 15～30 DEG C of temperature, and relative air humidity is80～95% natural lighting environmental condition is cultivated.
4. a breeding method for bee pupa Chinese caterpillar fungus, comprises the following steps:
(1) screening of bee pupa Cordyceps Militaris bacterial strain, obtains the method for Cordyceps strain spore application spore separationThe pure bacterial strain of Cordyceps sinensis;
(2) the organic culture medium of bee pupa is made: the live body protein 10 0g, large that adopts live body bee pupa or peeling bee pupa wormThin rice gruel 200g, camellia powder 10mg, potassium dihydrogen phosphate 2g, put into the beaker of 1000mL, add water 25～45mL to add thermal agitation miscible, completes after miscible and stops heating, and packing test tube is jumped a queue cooling, makes half liquidThe organic culture medium of bee pupa of body shape;
(3) making of spore suspension: the bee pupa Chinese caterpillar fungus strain that (1) step is cultivated is inoculated in (2) stepOn the organic culture medium of bee pupa of making, put at 20 DEG C and cultivate, after there is conidium, picking part is placed on nothingIn bacterium water, make spore suspension stand-by;
(4) host prepares: dry bee pupa is pulverized and made after bee pupa powder and honeycomb Extraction parts nutrimentLoose nest slag be mixed and made into culture medium, the spore suspension of making in step (3) is seeded in culture medium,Be placed at 15～30 DEG C of temperature, the natural lighting environmental condition that relative air humidity is 80～95% is cultivated;
(5) Collection and conservation of bee pupa Chinese caterpillar fungus: after stroma maturation, pick, be placed on and under 60 DEG C of environment, dry 2～4 hours, put shady and cool dry place sealing and preserve.
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| CN104770219A (en) * | 2015-04-21 | 2015-07-15 | 李天兰 | Method for preparing cordyceps militaris |
| CN105602787A (en) * | 2016-02-05 | 2016-05-25 | 陆九妹 | Bee pupa/Cordyceps militaris health-care wine, and preparation method and application thereof |
| CN111938022A (en) * | 2020-08-31 | 2020-11-17 | 辽宁爱普罗斯饲料有限公司 | Anti-bacterial feed additive adopting fungus solid state fermentation honeycomb residues and preparation and feeding method thereof |
| CN114747424B (en) * | 2022-02-23 | 2023-04-07 | 金华市农业科学研究院(浙江省农业机械研究院) | Mushroom culture medium with anti-aging effect |
| CN114793754A (en) * | 2022-04-15 | 2022-07-29 | 保山学院 | Method for culturing cordyceps sinensis by using drone |
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