CN104054429B - A kind of spring cuckoo seedling-cultivating method - Google Patents
A kind of spring cuckoo seedling-cultivating method Download PDFInfo
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- CN104054429B CN104054429B CN201410300200.9A CN201410300200A CN104054429B CN 104054429 B CN104054429 B CN 104054429B CN 201410300200 A CN201410300200 A CN 201410300200A CN 104054429 B CN104054429 B CN 104054429B
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- 241000544061 Cuculus canorus Species 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 20
- 238000002791 soaking Methods 0.000 claims abstract description 10
- 230000001954 sterilising effect Effects 0.000 claims abstract description 7
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 239000002775 capsule Substances 0.000 claims description 8
- 238000005286 illumination Methods 0.000 claims description 6
- 230000007226 seed germination Effects 0.000 abstract description 10
- 229940088597 hormone Drugs 0.000 abstract description 8
- 239000005556 hormone Substances 0.000 abstract description 8
- 238000004321 preservation Methods 0.000 abstract description 4
- 239000002609 medium Substances 0.000 description 15
- 241000208422 Rhododendron Species 0.000 description 11
- 241000196324 Embryophyta Species 0.000 description 10
- 230000012010 growth Effects 0.000 description 7
- YGGXZTQSGNFKPJ-UHFFFAOYSA-N methyl 2-naphthalen-1-ylacetate Chemical compound C1=CC=C2C(CC(=O)OC)=CC=CC2=C1 YGGXZTQSGNFKPJ-UHFFFAOYSA-N 0.000 description 7
- 230000035784 germination Effects 0.000 description 5
- 230000006698 induction Effects 0.000 description 5
- 241000245187 Rhododendron ferrugineum Species 0.000 description 3
- 235000000652 Rosa pendulina Nutrition 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 238000005406 washing Methods 0.000 description 3
- 241000208421 Ericaceae Species 0.000 description 2
- 241000196322 Marchantia Species 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 238000005336 cracking Methods 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 238000002360 preparation method Methods 0.000 description 2
- 230000008117 seed development Effects 0.000 description 2
- 230000035040 seed growth Effects 0.000 description 2
- 235000016626 Agrimonia eupatoria Nutrition 0.000 description 1
- 241000196323 Marchantiophyta Species 0.000 description 1
- 241001276618 Mayaca fluviatilis Species 0.000 description 1
- 241001617585 Rhododendron delavayi Species 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- 241000736285 Sphagnum Species 0.000 description 1
- 230000003044 adaptive effect Effects 0.000 description 1
- VQKFNUFAXTZWDK-UHFFFAOYSA-N alpha-methylfuran Natural products CC1=CC=CO1 VQKFNUFAXTZWDK-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000009402 cross-breeding Methods 0.000 description 1
- 239000002274 desiccant Substances 0.000 description 1
- 239000003599 detergent Substances 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000008030 elimination Effects 0.000 description 1
- 238000003379 elimination reaction Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000011081 inoculation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 238000011177 media preparation Methods 0.000 description 1
- 229960002523 mercuric chloride Drugs 0.000 description 1
- LWJROJCJINYWOX-UHFFFAOYSA-L mercury dichloride Chemical compound Cl[Hg]Cl LWJROJCJINYWOX-UHFFFAOYSA-L 0.000 description 1
- 238000000465 moulding Methods 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 230000001863 plant nutrition Effects 0.000 description 1
- 238000013138 pruning Methods 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 230000027272 reproductive process Effects 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 229960001866 silicon dioxide Drugs 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- UZKQTCBAMSWPJD-UQCOIBPSSA-N trans-Zeatin Natural products OCC(/C)=C\CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-UQCOIBPSSA-N 0.000 description 1
- UZKQTCBAMSWPJD-FARCUNLSSA-N trans-zeatin Chemical compound OCC(/C)=C/CNC1=NC=NC2=C1N=CN2 UZKQTCBAMSWPJD-FARCUNLSSA-N 0.000 description 1
- 229940023877 zeatin Drugs 0.000 description 1
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Abstract
The invention discloses the seedling-cultivating method of a kind of spring cuckoo, comprising: the seed getting spring cuckoo, after seed soaking, sterilization, is inoculated in seed on medium after having sterilized and cultivates; Is described medium for containing 1.0 ~ 2.0mg/L? the 1/4MS medium of ZT and 0.03 ~ 0.06mg/LNAA.The present invention adds the hormone of certain concentration and particular types in the medium, spring cuckoo seed germination can be impelled to form tufted seedling, improve reproduction coefficient, significant to the preservation of elite germplasm.In addition, the present invention adds the hormone of certain concentration and particular types in the medium, and seed can sprouting in about 10 days, and seed germination rate can up to about 48%.
Description
Technical field
The invention belongs to plant biotechnology field, particularly relate to the seedling-cultivating method of a kind of spring cuckoo.
Background technology
Azalea is Ericaceae (Ericaceae) Rhododendron (Rhododendron) plant, is one of large famous flower of China ten.China azalea is of a great variety, aboundresources, and interspecific difference is larger.The whole world about has azalea more than 1000 to plant, and China accounts for about 50%.Azalea is Woody flower, according to the difference in growth area, is evergreen, half evergreen and deciduous plant.General according to form, florescence, parent and source, azalea can be divided into the large class of a mao cuckoo, eastern cuckoo, Xia Juan and western cuckoo four (Huang Maoru and strong letter good 1984), traditionally, hair cuckoo and eastern cuckoo be collectively referred to as spring cuckoo.
Spring, cuckoo had very high ornamental value, large flower and brilliant color, and germination is strong, resistance to pruning.Plantation in blocks, its scenery is particularly grand.Be widely used in gardens, often do greening and use.Plant and all can form extremely beautiful scenery in grove, sylvan life, small stream limit, lawn and building are other.Potted plant and moulding cuckoo can be done simultaneously.
The modes of reproduction of azalea mainly contains cuttage, propagation by grafiting.But the seedling plant strain growth that seed obtains is healthy and strong, and well developed root system, vitality are vigorous, strong to adaptive capacity to environment, parent's merit can be kept, especially in the crossbreeding research of cuckoo, usually adopt seed to breed.
In existing seminal propagation research, for ensureing the breeding of cuckoo, the germination rate of common concern cuckoo seed, the survival rate etc. of plant, as, publication number is that the Chinese patent literature of CN101999291B discloses a kind of method for sowing alpine rhododendron by utilizing sphagnum, utilize the characteristic that bog moss and marchantia restricted mutually in vegetative period, can in alpine rose planting seed and process of growth, effective control marchantia growth, for alpine rose seed growth provides good ecotope, prevent cuckoo seedling because of the covering death of noble liverwort.Publication number is that the Chinese patent literature of CN1166271 discloses a kind of method for reproducing seeds of Rhododendron delavayi Franch, and the breeding being delavay rhododendron seed by improved culture medium matter provides good environmental condition, makes the reproduction rate of delavay rhododendron seed up to more than 70%.Publication number is the precision seedling-cultivating method that the Chinese patent literature of CN103299819A discloses a kind of alpine rose, environmental Kuznets Curves three steps after sprouting cultivation, the meticulous field planting of seed, field planting by the radicle of seed form, by controlling the condition of culture in each step, the seedling fostering time is short is only 60 ~ 65d, seedling early growth is neat, healthy and strong, and planting percent is up to 85 ~ 90%.But in the reproductive process of seed, each seed only can be sprouted and grow up to a plant, and reproduction coefficient is low, if take measures obtain multiple plant by a seed, so by greatly improving the reproductive efficiency of cuckoo, reduce costs, the preservation for elite germplasm is also significant.
Summary of the invention
The invention provides the seedling-cultivating method of a kind of spring cuckoo, seed germination can be induced to form tufted seedling.
Spring cuckoo a seedling-cultivating method, comprising:
Get the seed of spring cuckoo, after seed soaking, sterilization, is inoculated in seed on medium after having sterilized and cultivates; Described medium is the 1/4MS medium containing 1.0 ~ 2.0mg/LZT and 0.03 ~ 0.06mg/LNAA.
Spring cuckoo seed is placed in medium and cultivates by the application, and by adding ZT (zeatin) and NAA (methyl α-naphthyl acetate) in the medium, both actings in conjunction under suitable concentration, can make spring cuckoo seed germination form tufted seedling.
Preferably, in described medium, the concentration of described NAA is 0.05mg/L, and the concentration of described ZT is 1.0mg/L or 2.0mg/L.Under this concentration, all spring cuckoo seeds that can sprout all can form tufted seedling.The concentration of ZT is preferably 1.0mg/L, and under this concentration, the germination rate of seed is higher.
Described spring kind of cuckoo is ' The purple butterfly ', is ensure germination rate, and seed preferably takes from ripe uncracked green capsule.
Vernalization need first be soaked seed before cultivating, and seed soaking can impel spring cuckoo seed to germinate ahead of time, and during seed soaking, seed is placed in the water 20 ~ 30 hours of 18 ~ 23 DEG C, the temperature of water is 20 DEG C preferably, and the time of seed soaking is 24 hours preferably.
Suitable temperature and light is conducive to spring cuckoo seed germination and growth of seedling according to condition, and generally, the temperature of described cultivation is 22 ~ 25 DEG C, is preferably 24 DEG C.The illumination condition of described cultivation is: intensity of illumination is 2000 ~ 2500lx, and light application time is 12 ~ 14h/d, and preferably, intensity of illumination is 2500lx, light application time is 12h/d.
For ensureing the supply of plant nutrition, medium is cultivated a period of time (about 60 days), a replaceable subculture, the formula of medium is constant.
Compared with prior art, beneficial effect of the present invention is:
The present invention adds the hormone of certain concentration and particular types in the medium, spring cuckoo seed germination can be impelled to form tufted seedling, improve reproduction coefficient, significant to the preservation of elite germplasm.
Method of the present invention is easy and simple to handle, and seed is directly placed in medium and cultivates, culture medium prescription is simple.
The present invention adds the hormone of certain concentration and particular types in the medium, and seed can sprouting in about 10 days, and seed germination rate can up to about 48%.
Accompanying drawing explanation
Fig. 1 is control group growth of seedling situation, and wherein, A is not for add hormone, and B is 0.05mg/LNAA.
Fig. 2 is NAA and ZT synergy lower tufted seedling induction situation, and wherein, A is 0.05mg/LNAA, 1.0mg/LZT, and B is 0.05mg/LNAA, 2.0mg/LZT.
Fig. 3 is the situation schematic diagram of spring cuckoo seed at 0.05mg/LNAA+1.0mg/LZT+1/4MS medium induction tufted seedling.
Fig. 4 is growth of seedling situation under ZT independent role, and A is 1.0mg/LZT, B is 2.0mg/LZT.
Embodiment
Below in conjunction with specific embodiment, the present invention is further explained.
Embodiment 1
(1) seed preparation
Mid or late November, ripe at spring cuckoo ' The purple butterfly ' capsule, but be still uncracked green capsule time, adopted down, after liquid detergent water soaking 20-30min, under being placed on flowing water, rinsed 1-2h.Scrub the foul on capsule surface gently with banister brush after, blotting the moisture on capsule surface with filter paper, installing (without the need to covering culture dish lid) with culture dish.Being put into indoor is equipped with in the container of silica-gel desiccant, carries out drying, until capsule natural cracking.
(2) seed soaking
Before seed soaking, by the capsule strip off of cracking, without the need to carrying out disinfection to spring cuckoo seed.Select the seed of mature and plump after impurity elimination, place it in the warm water of 20 DEG C and soak 24h.
(3) medium preparation and sterilization
The medium of preparation containing hormon, horizontal high voltage sterilizing of going forward side by side (some hormone needs after autoclaving, joins in medium).
CK group:
①1/4MS②1/4MS+0.05mg/LNAA；
ZT group:
①1/4MS+0.05mg/LNAA+0.5mg/LKT②1/4MS+0.05mg/LNAA+1.0mg/LKT③1/4MS+0.05mg/LNAA+2.0mg/LKT④1/4MS+0.5mg/LKT⑤1/4MS+1.0mg/LKT⑥1/4MS+2.0mg/LKT；
(4) seed disinfection
Take out the seed soaked, blot the moisture of the surface of the seed with filter paper.First use 70% alcohol-pickled 30s, after aseptic water washing 3-5 time, seed is soaked in 15-18min in the mercuric chloride water of 0.1%, then uses aseptic water washing 3-5 time, each washing time is no less than 3min.Blot the moisture of the surface of the seed with filter paper after sterilization.
(5) aseptic seeding
The seed of sterilizing is inoculated in respectively in the medium containing variable concentrations, hormon, each process 30, repeats for 3 times.After inoculation, be positioned over that temperature is 24 DEG C, intensity of illumination is 2500lx, light application time is cultivate in the Sterile culture room of 12h/d.After cultivating 60d, change fresh culture, fill a prescription when culture medium prescription and seed germination identical.Observe the situation forming tufted seedling.
The situation of different disposal lower spring cuckoo Seed Development tufted seedling, in table 1, table 2.
Table 1CK group spring cuckoo seed germination and tufted seedling induction situation
| Hormone (mg/L) | Germination rate (%) | Sprout time (d) | Induction tufted seedling rate (%) |
| NAA0.05 | 58.9 | 11 | 0 |
| 1/4MS blank | 55 | 13 | 0 |
The situation that table 2 hormone ZT induces spring cuckoo seed germination and tufted seedling
As can be seen from data in table, the independent role of NAA and ZT, all can not make spring cuckoo Seed Development tufted seedling.When only having NAA and ZT with suitable concn (ZT is that 1mg/L or 2mg/L, NAA are for 0.05mg/L) acting in conjunction and spring cuckoo seed, spring cuckoo seed could form tufted seedling, and the ratio of induction tufted seedling is up to 100%.Each seed can induce tufted seedling 10-20 strain.The method can effectively induce spring cuckoo seed directly to form tufted seedling, and its method is easy, and formula is simple, and cost is low.Meanwhile, the preservation for elite germplasm is significant.
Claims (5)
1. the spring cuckoo a seedling-cultivating method, it is characterized in that, comprising:
Get the seed of spring cuckoo, after seed soaking, sterilization, is inoculated in seed on medium after having sterilized and cultivates; Described medium is the 1/4MS medium containing 1.0mg/LZT and 0.05mg/LNAA or 2.0mg/LZT and 0.05mg/LNAA.
2. seedling-cultivating method as claimed in claim 1, is characterized in that, described seed takes from the ripe uncracked green capsule of spring cuckoo.
3. seedling-cultivating method as claimed in claim 1, it is characterized in that, the method for described seed soaking comprises: the water 20 ~ 30 hours seed being placed in 18 ~ 23 DEG C.
4. seedling-cultivating method as claimed in claim 1, it is characterized in that, the temperature of described cultivation is 22 ~ 25 DEG C.
5. seedling-cultivating method as claimed in claim 1, it is characterized in that, the illumination condition of described cultivation is: intensity of illumination is 2000 ~ 2500lx, and light application time is 12 ~ 14h/d.
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| CN101095399A (en) * | 2006-06-29 | 2008-01-02 | 戴悦 | Protogenic alpine azalea introduction and acclimatization method |
| CN101695284A (en) * | 2009-10-29 | 2010-04-21 | 江苏省农业科学院 | Method for raising seedling rate of rhododendron hybrid seeds |
| CN102119594A (en) * | 2010-12-13 | 2011-07-13 | 江苏省农业科学院 | Method for promoting rhododendron parvifolium seed germination |
| CN102342246A (en) * | 2010-07-30 | 2012-02-08 | 贵州省生物研究所 | Rhododendron decorum tissue-culture quick propagation method |
| CN102792893A (en) * | 2012-08-27 | 2012-11-28 | 云南农业大学 | Tissue culture propagating method of Rhododendron agastum |
| CN103299819A (en) * | 2013-07-06 | 2013-09-18 | 云南省农业科学院花卉研究所 | Precise seedling growing method for alpine rhododendrons |
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- 2014-06-26 CN CN201410300200.9A patent/CN104054429B/en not_active Expired - Fee Related
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101095399A (en) * | 2006-06-29 | 2008-01-02 | 戴悦 | Protogenic alpine azalea introduction and acclimatization method |
| CN101695284A (en) * | 2009-10-29 | 2010-04-21 | 江苏省农业科学院 | Method for raising seedling rate of rhododendron hybrid seeds |
| CN102342246A (en) * | 2010-07-30 | 2012-02-08 | 贵州省生物研究所 | Rhododendron decorum tissue-culture quick propagation method |
| CN102119594A (en) * | 2010-12-13 | 2011-07-13 | 江苏省农业科学院 | Method for promoting rhododendron parvifolium seed germination |
| CN102792893A (en) * | 2012-08-27 | 2012-11-28 | 云南农业大学 | Tissue culture propagating method of Rhododendron agastum |
| CN103299819A (en) * | 2013-07-06 | 2013-09-18 | 云南省农业科学院花卉研究所 | Precise seedling growing method for alpine rhododendrons |
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| 大白杜鹃组培与快繁研究—基本外植体和初代培养基筛选;刘燕等;《种子》;20100831;第29卷(第8期);第34-37页 * |
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