CN104026132A - Method for preparing cold-resistance wheat conditioning agent - Google Patents
Method for preparing cold-resistance wheat conditioning agent Download PDFInfo
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- CN104026132A CN104026132A CN201410290668.4A CN201410290668A CN104026132A CN 104026132 A CN104026132 A CN 104026132A CN 201410290668 A CN201410290668 A CN 201410290668A CN 104026132 A CN104026132 A CN 104026132A
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Abstract
The invention belongs to the technical field of plant growth conditioning agents, and relates to a method for preparing a cold-resistance wheat conditioning agent. The method comprises the following steps: firstly, performing microwave degradation on enteromorpha polysaccharide prepared by using a hot water extraction method to prepare low molecular weight enteromorpha polysaccharide, further dissolving the prepared low molecular weight enteromorpha polysaccharide into distilled water so as to condition the concentration of enteromorpha polysaccharide to be 100-1000mg/L and prepare an enteromorpha polysaccharide solution, namely, the cold-resistance conditioning agent; or respectively adding salicylic acid, brassinolide, glycine betaine and/or spermidine into the enteromorpha polysaccharide solution, wherein the concentration of salicylic acid is 0mg/L or 100-200mg/L, the concentration of brassinolide is 0mg/L or 0.01-0.1mg/L, the concentration of glycine betaine is 0mg/L or 100-1000mg/L, and the concentration of spermidine is 0mg/L or 10-100mg/L, and finally uniformly stirring to prepare a compound solution, namely, the cold-resistance conditioning agent; or further adding a surfactant into the compound solution, wherein the concentration of the surfactant is 1000-20000mg/L, uniformly stirring so as to prepare the cold-resistance conditioning agent. The cold-resistance conditioning agent has the advantages of rich raw material resource, scientific preparation component, simple preparation process and low machining cost.
Description
Technical field:
The invention belongs to plant growth regulator technical field, relate to the cold-resistant conditioning agent preparation method of a grow wheat, component includes in sea grass polysaccharide or sea grass polysaccharide and salicylic acid, brassinosteroid, betain and/or spermidine one or more complex preparation, can effectively improve the cold tolerance of wheat.
Background technology:
Enteromorpha is a kind of large-scale economic green alga, belongs to Chlorophyta Ulvales Ulvaceae Enteromorpha, and frond is grass green, mainly grows in intertidal zone, has stronger fertility and environment tolerance, and all can grow the whole year, at China coast, has a large amount of distributions; But the green tide of extensive sudden Enteromorpha is broken out in China coastal seas bank marine site continuously in recent years, to marine environment, caused great pollution, at present to utilize method be to bury to make compost or dry to be ground into feed to common Enteromorpha mostly, its technology content is lower, utilization ratio is not high, caused the serious wasting of resources, therefore the Enteromorpha that needs exploitation to make new advances is processed and application technology, to strengthen the high-valued Application and Development of Enteromorpha, alleviates offshore pollution.The sea grass polysaccharide containing in Enteromorpha (EP) is one of Enteromorpha main active substances, it has numerous features such as immunoregulatory activity, short cell-proliferation activity and antioxidation activity, and sea grass polysaccharide is as pure natural algal polysaccharides, plant disease-resistant and degeneration-resistant aspect liquid there is far-reaching application potential.Salicylic acid (SA) is the disease-resistant and degeneration-resistant signal of interest molecule of inducing plant, can improve plant protection enzymic activity, increases osmotic adjustment and its secondary metabolites content, thereby improves disease resistance and the resistance of plant; Brassinosteroid (BR) is a kind of steroid compound that plant is had to growth regulating effect, can alleviate the injury effect of environment stress to plant seedlings, and it has extensive use as a kind of novel plant hormone on raising stress resistance of plant; Betain (GB) is a kind of quaternary ammonium type water-soluble alkaloid, is also a kind of atoxic osmotic adjustment, and it has obtained increasing attention in plant stress-resistance; Spermidine (Spd) is considered to similar second messenger's active substance, and the resistance of inducing plant is played an important role; Above-mentioned four kinds of materials are all nontoxic, harmless, pollution-free, can produce resistance by inducing plant, are that the biogenic chemistry of environmental protection lures anti-material.
Wheat is the second largest cereal crops of China, its breeding time is long, planted in fall, through Dong Lichun, gather in the crops summer, cross over 2 years breeding time, containing four seasons spring, summer, autumn and winter, at key developmental stages such as the early growth period of wheat and the jointing of turning green, all can experience the low temperature stress such as cold current, cold in the late spring, and nearly all winter wheat growing area has to damage to plants caused by sudden drop in temperature with freeze injury and occurs, the quality and yield that has greatly affected Wheat Production is stable, serious threat grain security, low temperature freezing-disaster has become one of natural calamity maximum in China's Wheat Production at present; Therefore, strengthen the anti-low temperature cold freeze injury of wheat ability to guaranteeing improving yield of wheat stable yields, Ensuring Food Safety has important practical significance and using value; In practice, there is Chinese patent 201310116703.6 to disclose anti-low temperature conditioning agent of a grow wheat and preparation method thereof, concrete component comprises glycosaminoglycan 3～9g/L, Choline Chloride 20～100g/L, salicylic acid or sodium salicylate 4～20g/L, silicon potash fertilizer 80～400g/L, surfactant 20～100g/L and water, but its complicated component, preparation method is loaded down with trivial details and poor effect; Therefore the present invention seeks to design the preparation method of a kind of simple and easy to get, with low cost, green natural and the cold-resistant conditioning agent of the significant wheat of anti-cryogenic effect, for the production of wheat, cultivates and has important economic implications.
Summary of the invention:
The object of the invention is to overcome the shortcoming that prior art exists, the preparation method of the cold-resistant conditioning agent of a grow wheat be provided, can strengthen wheat seed or seedling multinomial physical signs, improve multiple activities of antioxidant enzymes, improve the ability of wheat opposing low temperature stress.
To achieve these goals, a kind of preparation method of the cold-resistant conditioning agent of wheat the present invention relates to is: first the sea grass polysaccharide making with hot water extraction method is carried out to microwave degradation and make the low-molecular-weight sea grass polysaccharide that molecular weight is 1k-50kDa, again the low-molecular-weight sea grass polysaccharide of acquisition is dissolved in distilled water, making the concentration of sea grass polysaccharide is 100-1000mg/L, is mixed with sea grass polysaccharide solution and makes cold-resistant conditioning agent.
The another kind of preparation method of the cold-resistant conditioning agent of wheat the present invention relates to is: low-molecular-weight sea grass polysaccharide is dissolved in and in distilled water, is mixed with the sea grass polysaccharide solution that concentration is 100-1000mg/L, in sea grass polysaccharide solution, add respectively salicylic acid again, brassinosteroid, betain and/or spermidine, making Determination of Salicylic Acid is 0mg/L or 100-200mg/L, brassinosteroid concentration is 0mg/L or 0.01-0.1mg/L, betain concentration is 0mg/L or 100-1000mg/L, spermidine concentration is 0mg/L or 10-100mg/L, the proportioning that finally stirs is made combination solution and is made cold-resistant conditioning agent.
The another kind of preparation method of the cold-resistant conditioning agent of wheat the present invention relates to is: low-molecular-weight sea grass polysaccharide is dissolved in and in distilled water, is mixed with the sea grass polysaccharide solution that concentration is 100-1000mg/L, in sea grass polysaccharide solution, add respectively salicylic acid again, brassinosteroid, betain and/or spermidine, making Determination of Salicylic Acid is 0mg/L or 100-200mg/L, brassinosteroid concentration is 0mg/L or 0.01-0.1mg/L, betain concentration is 0mg/L or 100-1000mg/L, spermidine concentration is 0mg/L or 10-100mg/L, the proportioning that finally stirs is made combination solution, in combination solution, add surfactant again, making surfactant concentration is 1000-20000mg/L, after stirring, make cold-resistant conditioning agent.
The surfactant the present invention relates to is tween series, sapn is serial, OP is serial and/or one or more the mixture of NP in serial; The using method of the cold-resistant conditioning agent of wheat making is for to spray/to irrigate wheat seedling, or wheat seed is soaked seed after 6-12h and sowed.
The present invention compares with product with prior art, lure anti-material to carry out the biogenic such as sea grass polysaccharide and salicylic acid, brassinosteroid, betain and spermidine composite, make cold-resistant conditioning agent, can improve the comprehensive resistance of plant to low temperature stress, guarantee plant normal growth growth; Selected raw material Enteromorpha source is abundant, with low cost, environmental friendliness; The method of preparing conditioning agent is simple, easy to utilize.
Embodiment:
Below by embodiment, the present invention is described in further detail, but the present invention is not limited to following embodiment.
Embodiment 1:
In the present embodiment, first the sea grass polysaccharide making with hot water extraction method is carried out to microwave degradation and make the low-molecular-weight sea grass polysaccharide that molecular weight is 1k-50kDa, again the low-molecular-weight sea grass polysaccharide of acquisition is dissolved in distilled water, making sea grass polysaccharide concentration is 100-1000mg/L, makes cold-resistant conditioning agent A; Or again to add respectively in cold-resistant conditioning agent A salicylic acid, brassinosteroid, betain and/spermidine wherein any one or multiple, concrete concentration is salicylic acid 0mg/L or 100-200mg/L, brassinosteroid 0mg/L or 0.01-0.1mg/L, betain 0mg/L or 100-1000mg/L, spermidine 0mg/L or 10-100mg/L, make cold-resistant conditioning agent B; Or in cold-resistant conditioning agent A or cold-resistant conditioning agent B, add respectively surfactant again, surfactant is selected tween series, sapn is serial, OP is serial and/or one or more the mixture of NP in serial, making surfactant concentration is 1-20g/L, makes cold-resistant conditioning agent C; Above-mentioned cold-resistant conditioning agent A, cold-resistant conditioning agent B and cold-resistant conditioning agent C all can effectively strengthen every physical signs of wheat seed or seedling, and improve multiple activities of antioxidant enzymes, to promote the cold tolerance of wheat, promote its growth.
Embodiment 2:
The sea grass polysaccharide that first takes 100mg molecular weight in the present embodiment and be 5kDa is dissolved in 800ml distilled water, then adds 1000mg Tween-20 and stir, and finally with distilled water, is settled to 1L, makes the cold-resistant conditioning agent of wheat after stirring.
Embodiment 3:
The sea grass polysaccharide that first takes 100mg molecular weight in the present embodiment and be 5kDa is dissolved in and in 800ml distilled water, makes sea grass polysaccharide solution, taking 100mg salicylic acid is dissolved in above-mentioned sea grass polysaccharide solution again, then add 1000mg Tween-20 and stir, finally with distilled water, be settled to 1L, after stirring, make the cold-resistant conditioning agent of wheat.
Embodiment 4:
The sea grass polysaccharide that first takes 100mg molecular weight in the present embodiment and be 5kDa is dissolved in and in 800ml distilled water, makes sea grass polysaccharide solution, taking 0.01mg brassinosteroid is dissolved in above-mentioned sea grass polysaccharide solution again, then add 5000mg Arlacel-60 and stir, finally with distilled water, be settled to 1L, after stirring, make the cold-resistant conditioning agent of wheat.
Embodiment 5:
The sea grass polysaccharide that first takes 100mg molecular weight in the present embodiment and be 50kDa is dissolved in and in 800ml distilled water, makes sea grass polysaccharide solution, taking 10mg spermidine is dissolved in above-mentioned sea grass polysaccharide solution again, then add 5000mg OP-7 and stir, finally with distilled water, be settled to 1L, after stirring, make the cold-resistant conditioning agent of wheat.
Embodiment 6:
The sea grass polysaccharide that first takes 1000mg molecular weight in the present embodiment and be 5kDa is dissolved in and in 800ml distilled water, makes sea grass polysaccharide solution, taking 100mg betain is dissolved in above-mentioned sea grass polysaccharide solution again, then add 5000mg NP-7 and stir, finally with distilled water, be settled to 1L, after stirring, make the cold-resistant conditioning agent of wheat.
Embodiment 7:
The present embodiment sprays processing to the wheat seedling in Seedling Stage with the cold-resistant conditioning agent making in embodiment 2-6, and specific experiment is as follows::
(1) experimental technique: by wheat seed sterilization, immersion, vernalization, treat that seed just shows money or valuables one carries unintentionally, selecting full, uniform wheat seed sows in culture dish, 20, every ware, with Hoagland (Gram suddenly) nutrient solution, in illumination box, cultivate, condition of culture is 25/20 ℃ (daytime/night), intensity of illumination 300 μ mol/m2/s, periodicity of illumination 14/l0h (daytime/night), relative moisture 65 ± 5%; Until wheat seedling growth to 2 leaf, during 1 heart stage, with the cold-resistant conditioning agent making in embodiment 2-6, wheat seedlings blade is sprayed, make blade completely moistening, but liquid do not flow down, negative control group spray is with the clear water of equivalent; After 24h, wheat seedling is placed in to 4 ℃ of low temperature stress 3d, then measures wheat seedlings blade indices;
(2) experimental result: 1. malonaldehyde (MDA) is the product of film lipid peroxidation under salt stress, and the large I of its content represents the size of membrane damage degree; In this experiment, under low temperature stress, MDA content significantly raises, and can obviously alleviate the injury of low temperature stress cell membrane after cold-resistant conditioning agent is processed; Under low temperature stress condition, wheat seedlings blade soluble sugar and soluble protein content obviously reduce, and after cold-resistant conditioning agent is processed, can improve the two content, improve the overall metaboilic level of plant corpus, and maintain plant osmosis regulating power; Cold-resistant conditioning agent can also improve wheat seedlings blade chlorophyll content, to urge photosynthesis, guarantees plant normal growth growth; Concrete comparing result is as shown in table 1:
The impact of the cold-resistant conditioning agent of table 1 wheat on wheat seedlings blade indices
2. superoxide dismutase (SOD), peroxidase (POD) and peroxidase (CAT) form the protective enzyme system of plant jointly, can catalytic activity oxygen generation disproportionated reaction generate nontoxic molecular oxygen and water; In this test, under the stimulation of low temperature stress, the activity of SOD and CAT increases, with protective plant cell membrane, after cold-resistant conditioning agent is processed, SOD, CAT and POD are active significantly to raise, and is not subject to the damage of free radical with Cell protection film, improves the resistivity of plant to low temperature stress; Concrete comparing result is as shown in table 2:
The impact of the cold-resistant conditioning agent of table 2 wheat on wheat seedlings blade antioxidase
3. this experiment, after low temperature stress 3d, moves to wheat seedling under normal temperature condition and cultivates 7d, then measures its height of seedling, weight in wet base; Then use after 105 ℃ of oven for drying 2h to constant weight, then measure dry weight; Result shows that the height of seedling of wheat seedling, seedling weight average are significantly increased after cold-resistant conditioning agent is processed, and illustrates that sea grass polysaccharide can improve the ability of wheat seedling opposing low temperature stress, to guarantee its normal growth; Concrete comparing result is as shown in table 3:
The impact of the cold-resistant conditioning agent of table 3 wheat on wheat seedling biomass
Claims (3)
1. the cold-resistant conditioning agent preparation method of a grow wheat, is characterized in that concrete steps are:
First the sea grass polysaccharide making with hot water extraction method is carried out to microwave degradation and make the low-molecular-weight sea grass polysaccharide that molecular weight is 1k-50kDa, again the low-molecular-weight sea grass polysaccharide of acquisition is dissolved in distilled water, making the concentration of sea grass polysaccharide is 100-1000mg/L, is mixed with sea grass polysaccharide solution and makes cold-resistant conditioning agent; Or in sea grass polysaccharide solution, add respectively salicylic acid, brassinosteroid, betain and/or spermidine again, making Determination of Salicylic Acid is 0mg/L or 100-200mg/L, brassinosteroid concentration is 0mg/L or 0.01-0.1mg/L, betain concentration is 0mg/L or 100-1000mg/L, spermidine concentration is 0mg/L or 10-100mg/L, and the proportioning that finally stirs is made combination solution and made cold-resistant conditioning agent; Or in combination solution, add surfactant again, making surfactant concentration is 1000-20000mg/L, makes cold-resistant conditioning agent after stirring.
2. the cold-resistant conditioning agent preparation method of wheat according to claim 1, is characterized in that described surfactant is tween series, sapn is serial, OP is serial and/or one or more the mixture of NP in serial.
3. the cold-resistant conditioning agent preparation method of wheat according to claim 1, it is characterized in that the cold-resistant conditioning agent of wheat that the method makes can effectively strengthen every physical signs of wheat seed or seedling, and improve multiple activities of antioxidant enzymes, to promote the cold tolerance of wheat, promote its growth.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104396547A (en) * | 2014-12-11 | 2015-03-11 | 李润霞 | Method for improving chilling resistance of plant via glycine betaine |
| CN107183050A (en) * | 2017-05-27 | 2017-09-22 | 兰溪市酉泽饲料技术服务有限公司 | Wheat low-temperature resistance conditioning agent and its application method |
| CN111011394A (en) * | 2019-12-12 | 2020-04-17 | 山东省农作物种质资源中心 | Wheat cold-resistant inducer and application method thereof |
| CN114304157A (en) * | 2020-10-10 | 2022-04-12 | 中国农业大学 | High-temperature-resistant grouting-promoting plant growth regulator and application thereof to crops |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487943A (en) * | 2011-12-14 | 2012-06-13 | 中国科学院遗传与发育生物学研究所 | Composition capable of increasing wheat yield, method of preparing composition and application of composition |
| CN102898536A (en) * | 2012-10-19 | 2013-01-30 | 中国科学院海洋研究所 | Method for degrading enteromorpha polysaccharide |
| CN103202315A (en) * | 2013-04-07 | 2013-07-17 | 江苏徐淮地区淮阴农业科学研究所 | Low-temperature-resistant modifier of wheat and preparation method of modifier |
-
2014
- 2014-06-25 CN CN201410290668.4A patent/CN104026132B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102487943A (en) * | 2011-12-14 | 2012-06-13 | 中国科学院遗传与发育生物学研究所 | Composition capable of increasing wheat yield, method of preparing composition and application of composition |
| CN102898536A (en) * | 2012-10-19 | 2013-01-30 | 中国科学院海洋研究所 | Method for degrading enteromorpha polysaccharide |
| CN103202315A (en) * | 2013-04-07 | 2013-07-17 | 江苏徐淮地区淮阴农业科学研究所 | Low-temperature-resistant modifier of wheat and preparation method of modifier |
Non-Patent Citations (5)
| Title |
|---|
| BING LI ET AL.: "Degradation of sulfated polysaccharides from Enteromorpha prolifera and their antioxidant activities", 《CARBOHYDRATE POLYMERS》, vol. 92, 3 December 2012 (2012-12-03), pages 1991 - 1996 * |
| 冯玉磊 等: "寒地冬小麦返青期抗氧化特性", 《麦类作物学报》, vol. 32, no. 3, 31 December 2012 (2012-12-31), pages 547 - 550 * |
| 古红梅 等: "亚精胺对小麦幼苗耐冷性的影响", 《种子》, no. 4, 31 December 2003 (2003-12-31), pages 26 - 28 * |
| 张煜星 等: "表油菜素内酯对小麦幼苗抗冷性的影响", 《石河子农学院学报》, no. 2, 31 December 1995 (1995-12-31), pages 15 - 18 * |
| 齐付国 等: "水杨酸对小麦幼苗抗寒性的影响", 《安徽农业科学》, vol. 36, no. 14, 31 December 2008 (2008-12-31), pages 5732 - 5733 * |
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104396547A (en) * | 2014-12-11 | 2015-03-11 | 李润霞 | Method for improving chilling resistance of plant via glycine betaine |
| CN104396547B (en) * | 2014-12-11 | 2017-01-11 | 朱小丽 | Method for improving chilling resistance of plant via glycine betaine |
| CN107183050A (en) * | 2017-05-27 | 2017-09-22 | 兰溪市酉泽饲料技术服务有限公司 | Wheat low-temperature resistance conditioning agent and its application method |
| CN111011394A (en) * | 2019-12-12 | 2020-04-17 | 山东省农作物种质资源中心 | Wheat cold-resistant inducer and application method thereof |
| CN111011394B (en) * | 2019-12-12 | 2021-02-02 | 山东省农作物种质资源中心 | Wheat cold-resistant inducer and application method thereof |
| CN114304157A (en) * | 2020-10-10 | 2022-04-12 | 中国农业大学 | High-temperature-resistant grouting-promoting plant growth regulator and application thereof to crops |
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Effective date of registration: 20211014 Address after: 124010 Room 303, Xinglongtai District building economic incubation base, No. 52, Hexiang Road, Chuangxin street, Xinglongtai District, Panjin City, Liaoning Province Patentee after: Zhongke Institute of Marine Biology (Panjin) Co.,Ltd. Address before: 266000 Shandong province Qingdao City Nanhai Road No. 7 Patentee before: Institute of Oceanology Chinese Academy of Sciences |