CH561779A5 - Culture appts - for micro-organisms and tissue cells - Google Patents
Culture appts - for micro-organisms and tissue cellsInfo
- Publication number
- CH561779A5 CH561779A5 CH645772A CH645772A CH561779A5 CH 561779 A5 CH561779 A5 CH 561779A5 CH 645772 A CH645772 A CH 645772A CH 645772 A CH645772 A CH 645772A CH 561779 A5 CH561779 A5 CH 561779A5
- Authority
- CH
- Switzerland
- Prior art keywords
- container
- glass
- wound surface
- tissue cells
- spiral
- Prior art date
Links
- 244000005700 microbiome Species 0.000 title claims description 3
- 239000011521 glass Substances 0.000 claims abstract description 6
- 239000004033 plastic Substances 0.000 claims abstract description 5
- 229920003023 plastic Polymers 0.000 claims abstract description 5
- 239000004417 polycarbonate Substances 0.000 claims abstract description 5
- 229920000515 polycarbonate Polymers 0.000 claims abstract description 5
- 238000004113 cell culture Methods 0.000 claims description 4
- -1 Polyfluoroethylene Polymers 0.000 claims description 2
- 230000010261 cell growth Effects 0.000 claims description 2
- 238000004140 cleaning Methods 0.000 claims description 2
- 238000012258 culturing Methods 0.000 claims description 2
- 238000004381 surface treatment Methods 0.000 claims description 2
- 239000000463 material Substances 0.000 abstract 2
- 229910000831 Steel Inorganic materials 0.000 abstract 1
- 238000001816 cooling Methods 0.000 abstract 1
- 210000003298 dental enamel Anatomy 0.000 abstract 1
- 210000004907 gland Anatomy 0.000 abstract 1
- 238000010438 heat treatment Methods 0.000 abstract 1
- 230000036512 infertility Effects 0.000 abstract 1
- 239000005368 silicate glass Substances 0.000 abstract 1
- 239000010959 steel Substances 0.000 abstract 1
- 230000001954 sterilising effect Effects 0.000 abstract 1
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 238000004804 winding Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
- C12M27/06—Stirrer or mobile mixing elements with horizontal or inclined stirrer shaft or axis
Landscapes
- Wood Science & Technology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Within the main vessel used for prepn of the culture is a rotor with a spiral surface based on winding sheet material about the horizontal shaft so that there is a constant gap between adjacent turns and then by enclosing the ends between cheeks with holes at the radius of the inmost layer. The shaft is esp. hollow with pipework for circulating heating or cooling media and is driven from outside the vessel at variable speed and direction the shaft gland being a sterile seal. The material of the spiral may be plastic, esp. polycarbonate, silicate glass or steel covered with glass enamel. Alternatively the rotor may consist of a number of spiral coils of glass tubing laid against each other and welded together. Ease of sterilization between batches and of maintaining sterility.
Description
Die Erfindung betrifft eine weitere Ausführungsform der im Patentanspruch II des Hauptpatentes beschriebenen Vorrichtung, welche aus einer spiralförmig aufgewickelten Fläche besteht, die um ihre horizontale Achse in einem Behälter rotierbar ist. Es hat sich gezeigt, dass die zur Zellzüchtung erstrebten Monolayen > -Schichten, d.h. hautartige, nur aus einer Zellschicht bestehende Kulturen, nicht aufjeder Unterlage wachsen. Glas hat sich bewährt, ist jedoch schwierig in die erforderliche Spiralform zu bringen. Organische Kunststoffe, welche physiologisch einwandfrei sind, z.B. Polyfluoräthylen müssen erst durch spezielle Oberflächenbehandlung für diesen Zweck hergerichtet werden, um ein genügendes Haften der Zellkultur zu ermöglichen.
Gegenstand der Zusatzerfindung ist es, dass in einer Vorrichtung gemäss dem Patentanspruch II des Hauptpatentes die spiralig aufgewickelte Fläche aus Polycarbonat besteht und in einem liegenden Behälter eingebaut ist.
Dieser Kunststoff hat eine glatte Oberfläche, wie sie für die erforderliche Reinigung nötig ist, andererseits die erwünschte Eigenschaft, ähnlich wie Glas, den wachsenden Gewebezellen ausreichende Adhäsion zu bieten, damit die Zellkultur in einer Schicht wächst
PATENTANSPRUCH
Vorrichtung zur Züchtung von Gewebezellen und Mikroorganismen auf einer spiralförmig aufgewickelten Fläche, die in einem Behälter um ihre horizontale Achse rotierbar angeordnet ist, gemäss Patentanspruch II des Hauptpatentes, dadurch gekennzeichnet, dass die spiralig aufgewickelte Fläche aus Polycarbonat besteht und in einem liegenden Behälter eingebaut ist.
**WARNUNG** Ende DESC Feld konnte Anfang CLMS uberlappen**.
The invention relates to a further embodiment of the device described in claim II of the main patent, which consists of a spirally wound surface which can be rotated about its horizontal axis in a container. It has been shown that the Monolayen> -layers aimed at for cell growth, i.e. skin-like cultures consisting of only one layer of cells do not grow on any surface. Glass has proven itself, but it is difficult to bring the required spiral shape. Organic plastics that are physiologically sound, e.g. Polyfluoroethylene must first be prepared for this purpose through a special surface treatment in order to enable the cell culture to adhere sufficiently.
The object of the additional invention is that in a device according to claim II of the main patent, the spirally wound surface consists of polycarbonate and is installed in a horizontal container.
This plastic has a smooth surface, which is necessary for the required cleaning, on the other hand the desired property, similar to glass, of offering the growing tissue cells sufficient adhesion so that the cell culture grows in one layer
PATENT CLAIM
Device for culturing tissue cells and microorganisms on a spiral wound surface which is rotatably arranged in a container about its horizontal axis, according to claim II of the main patent, characterized in that the spirally wound surface consists of polycarbonate and is built into a horizontal container.
** WARNING ** End of DESC field could overlap beginning of CLMS **.
Claims (1)
Priority Applications (10)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH645772A CH561779A5 (en) | 1972-04-28 | 1972-04-28 | Culture appts - for micro-organisms and tissue cells |
| US00274063A US3812016A (en) | 1971-08-13 | 1972-07-21 | Culture-growing apparatus |
| DE2236240A DE2236240C3 (en) | 1971-08-13 | 1972-07-24 | Device for growing tissue cells and microorganisms |
| CA149,099A CA973825A (en) | 1971-08-13 | 1972-08-10 | Culture-growing apparatus |
| GB3742272A GB1366588A (en) | 1971-08-13 | 1972-08-10 | Apparatus for cultivating cells and microorganisms |
| SE7210377A SE376439B (en) | 1971-08-13 | 1972-08-10 | |
| IT28105/72A IT963945B (en) | 1971-08-13 | 1972-08-11 | DEVICE FOR THE CULTICATION OF TISSUE CELLS AND MICROOR GANISMS |
| DD165028A DD98112A5 (en) | 1971-08-13 | 1972-08-11 | |
| FR7229758A FR2149578B1 (en) | 1971-08-13 | 1972-08-11 | |
| US00338509A US3778353A (en) | 1971-08-13 | 1973-03-06 | Removing a cell culture from a carrier |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CH645772A CH561779A5 (en) | 1972-04-28 | 1972-04-28 | Culture appts - for micro-organisms and tissue cells |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CH561779A5 true CH561779A5 (en) | 1975-05-15 |
Family
ID=4309941
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CH645772A CH561779A5 (en) | 1971-08-13 | 1972-04-28 | Culture appts - for micro-organisms and tissue cells |
Country Status (1)
| Country | Link |
|---|---|
| CH (1) | CH561779A5 (en) |
-
1972
- 1972-04-28 CH CH645772A patent/CH561779A5/en not_active IP Right Cessation
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PLZ | Patent of addition ceased | ||
| PL | Patent ceased |