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CA2363297C - Mise au point de voies de sialylation intracellulaires - Google Patents

Mise au point de voies de sialylation intracellulaires Download PDF

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Publication number
CA2363297C
CA2363297C CA2363297A CA2363297A CA2363297C CA 2363297 C CA2363297 C CA 2363297C CA 2363297 A CA2363297 A CA 2363297A CA 2363297 A CA2363297 A CA 2363297A CA 2363297 C CA2363297 C CA 2363297C
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CA
Canada
Prior art keywords
cell
cmp
cells
leu
lys
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA2363297A
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English (en)
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CA2363297A1 (fr
Inventor
Michael J. Betenbaugh
Shawn Lawrence
Yuan C. Lee
Timothy A. Coleman
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Human Genome Sciences Inc
Johns Hopkins University
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Human Genome Sciences Inc
Johns Hopkins University
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Publication of CA2363297A1 publication Critical patent/CA2363297A1/fr
Application granted granted Critical
Publication of CA2363297C publication Critical patent/CA2363297C/fr
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Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01052—Beta-N-acetylhexosaminidase (3.2.1.52)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1048—Glycosyltransferases (2.4)
    • C12N9/1081—Glycosyltransferases (2.4) transferring other glycosyl groups (2.4.99)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/90—Isomerases (5.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2799/00—Uses of viruses
    • C12N2799/02—Uses of viruses as vector
    • C12N2799/021—Uses of viruses as vector for the expression of a heterologous nucleic acid
    • C12N2799/026—Uses of viruses as vector for the expression of a heterologous nucleic acid where the vector is derived from a baculovirus

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

L'invention concerne des méthodes de manipulation des voies de traitement des glucides dans des cellules étudiées. Ces méthodes consistent à manipuler plusieurs voies impliquées dans la réaction de sialylation par utilisation des techniques de l'ADN de recombinaison et de l'alimentation en substrat, de manière à permettre la production des glycoprotéines sialylées dans les cellules étudiées. Parmi ces travaux de manipulation réalisés sur les glucides, figurent la mise en oeuvre de nouveaux dosages biologiques, l'examen d'une sélection de lignées cellulaires d'insectes et l'utilisation de la bioinformatique pour l'identification de séquences géniques destinées au traitement critique des enzymes. L'invention concerne également des compositions renfermant les cellules étudiées produisant des glycoprotéines sialylées. Ces méthodes et ces compositions sont utilisées pour l'expression hétérologue des glycoprotéines.
CA2363297A 1999-03-02 2000-03-01 Mise au point de voies de sialylation intracellulaires Expired - Lifetime CA2363297C (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US12258299P 1999-03-02 1999-03-02
US60/122,582 1999-03-02
US16962499P 1999-12-08 1999-12-08
US60/169,624 1999-12-08
PCT/US2000/005313 WO2000052135A2 (fr) 1999-03-02 2000-03-01 Mise au point de voies de sialylation intracellulaires

Publications (2)

Publication Number Publication Date
CA2363297A1 CA2363297A1 (fr) 2000-09-08
CA2363297C true CA2363297C (fr) 2011-08-09

Family

ID=26820692

Family Applications (1)

Application Number Title Priority Date Filing Date
CA2363297A Expired - Lifetime CA2363297C (fr) 1999-03-02 2000-03-01 Mise au point de voies de sialylation intracellulaires

Country Status (5)

Country Link
EP (1) EP1399538A4 (fr)
JP (1) JP2003524395A (fr)
AU (1) AU3508300A (fr)
CA (1) CA2363297C (fr)
WO (1) WO2000052135A2 (fr)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3150699B2 (fr) † 2014-05-31 2024-08-21 JCR Pharmaceuticals CO., LTD. Milieu contenant de l'uridine et de la n-acétyl-d-mannosamine

Families Citing this family (21)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6949372B2 (en) 1999-03-02 2005-09-27 The Johns Hopkins University Engineering intracellular sialylation pathways
AU2001236739A1 (en) * 2000-02-08 2001-08-20 Genentech Inc. Improved sialylation of glycoproteins
US7863020B2 (en) * 2000-06-28 2011-01-04 Glycofi, Inc. Production of sialylated N-glycans in lower eukaryotes
US20040265954A1 (en) 2002-03-07 2004-12-30 Markus Aebi System and method for the production of recombinant proteins
WO2003074687A1 (fr) * 2002-03-07 2003-09-12 Eidgenössische Technische Hochschule Zürich Systeme et procede de fabrication de proteines glycosylees de recombinaison dans un hote procaryotique
JP2006333701A (ja) * 2003-01-15 2006-12-14 Kazuhito Fujiyama 動物型糖鎖をもつ糖タンパク質の生産方法
CN1926242B (zh) * 2004-02-13 2013-01-16 格莱克托普有限公司 高活性糖蛋白加工条件及其有效生产方法
AU2005224672B2 (en) * 2004-03-17 2011-06-02 Glycofi, Inc. Method of engineering a cytidine monophosphate-sialic acid synthetic pathway in fungi and yeast
SG152290A1 (en) * 2004-05-04 2009-05-29 Univ Singapore Method for expressing sialylated glycoproteins in mammalian cells and cells thereof
US7479549B2 (en) 2005-02-23 2009-01-20 Jaques John Scott T Recombinant canine thyroid stimulating hormone and methods of production and use thereof
EP2853600B1 (fr) 2005-05-11 2018-09-19 ETH Zürich Proteines N-glycosylées de recombinaison produites a partir de cellules procaryotes
PT1904636E (pt) 2005-07-12 2010-09-14 Greenovation Biotech Gmbh Melhoramentos na ou relacionados com a produção de proteínas
EP2073842B2 (fr) 2006-09-10 2023-10-18 Glycotope GmbH Utilisation des cellules humaines provenant d'une leucémie myéloïde pour l'expression d'anticorps
PL1920781T3 (pl) 2006-11-10 2015-06-30 Glycotope Gmbh Kompozycje zawierające core-1-dodatnie mikroorganizmy i ich zastosowanie w leczeniu lub profilaktyce nowotworów
CN101983070B (zh) 2008-02-20 2016-03-30 格林考瓦因有限公司 由来源于原核细胞的重组n-糖基化蛋白制备生物共轭物
PT2501406T (pt) 2009-11-19 2018-02-21 Glaxosmithkline Biologicals Sa Sistema de biossíntese que produz polissacáridos imunogénicos em células procariotas
PT2566507T (pt) 2010-05-06 2018-02-06 Glaxosmithkline Biologicals Sa Vacinas de bioconjugado de bactérias gram-positivas capsulares
EP2412815A1 (fr) 2010-07-27 2012-02-01 Universite De Rouen N-glycosylation dans le phaeodctylum tricornutum transformé
JP5918697B2 (ja) * 2010-10-15 2016-05-18 Ｊｃｒファーマ株式会社 糖鎖の非還元末端がマンノース残基である糖蛋白質の製造方法
CN103814123A (zh) 2011-08-22 2014-05-21 葛莱高托普有限公司 携带肿瘤抗原的微生物
RS64379B1 (sr) 2018-05-18 2023-08-31 Glycotope Gmbh Anti-muc1 antitelo

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3150699B2 (fr) † 2014-05-31 2024-08-21 JCR Pharmaceuticals CO., LTD. Milieu contenant de l'uridine et de la n-acétyl-d-mannosamine

Also Published As

Publication number Publication date
WO2000052135A3 (fr) 2004-01-08
EP1399538A2 (fr) 2004-03-24
AU3508300A (en) 2000-09-21
CA2363297A1 (fr) 2000-09-08
WO2000052135A9 (fr) 2001-10-11
EP1399538A4 (fr) 2004-03-24
JP2003524395A (ja) 2003-08-19
WO2000052135A2 (fr) 2000-09-08

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