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CA2284066A1 - Methodes de tri de composes au moyen de cellules encapsulees - Google Patents

Methodes de tri de composes au moyen de cellules encapsulees Download PDF

Info

Publication number
CA2284066A1
CA2284066A1 CA002284066A CA2284066A CA2284066A1 CA 2284066 A1 CA2284066 A1 CA 2284066A1 CA 002284066 A CA002284066 A CA 002284066A CA 2284066 A CA2284066 A CA 2284066A CA 2284066 A1 CA2284066 A1 CA 2284066A1
Authority
CA
Canada
Prior art keywords
assay system
producing species
cell
natural products
screening
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
CA002284066A
Other languages
English (en)
Inventor
Nicole M. Nasby
Todd C. Peterson
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Chromaxome Corp
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Publication of CA2284066A1 publication Critical patent/CA2284066A1/fr
Abandoned legal-status Critical Current

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G01N33/502—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics for testing non-proliferative effects
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G01N33/5008—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells for testing or evaluating the effect of chemical or biological compounds, e.g. drugs, cosmetics
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/195—Assays involving biological materials from specific organisms or of a specific nature from bacteria
    • G01N2333/36—Assays involving biological materials from specific organisms or of a specific nature from bacteria from Actinomyces; from Streptomyces (G)

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Hematology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Toxicology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La présente invention concerne une méthode intégrée de tri de médicaments conçue pour coupler un dosage de tri temporel et spatial à une synthèse de produits naturels dans un micro-organisme. La présente invention décrit une unité de tri qui consiste en une gouttelette de gel comprenant une espèce productive qui produit des produits naturels destinés au tri de médicaments, et un système de dosage qui détecte ou mesure une activité biologique voulue. Une espèce productive est co-encapsulée avec un système de dosage dans une unité de tri lorsque l'espèce productive est à une phase de son cycle vital optimale pour la production de produits naturels, tels que des métabolites secondaires. L'espèce productive est disposée dans l'espace par rapport au système de dosage dans la même unité de telle manière que des composés produits par l'espèce productive puissent entrer en contact avec le système de dosage. Si un composé possède l'activité voulue, le système de dosage génère un signal permettant l'identification et/ou l'isolation de l'unité de tri. La présente invention concerne également des méthodes de formation d'une unité de tri, ainsi que des méthodes d'utilisation d'une unité de tri pour trier des médicaments. Les méthodes selon l'invention s'utilisent pour trier des organismes naturels, et des excipients solides contenant des produits chimiques.
CA002284066A 1997-03-18 1998-03-18 Methodes de tri de composes au moyen de cellules encapsulees Abandoned CA2284066A1 (fr)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US4088897P 1997-03-18 1997-03-18
US60/040,888 1997-03-18
US7104698P 1998-01-13 1998-01-13
US60/071,046 1998-01-13
PCT/US1998/005462 WO1998041869A1 (fr) 1997-03-18 1998-03-18 Methodes de tri de composes au moyen de cellules encapsulees

Publications (1)

Publication Number Publication Date
CA2284066A1 true CA2284066A1 (fr) 1998-09-24

Family

ID=26717558

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002284066A Abandoned CA2284066A1 (fr) 1997-03-18 1998-03-18 Methodes de tri de composes au moyen de cellules encapsulees

Country Status (4)

Country Link
EP (1) EP0975969A4 (fr)
AU (1) AU6571598A (fr)
CA (1) CA2284066A1 (fr)
WO (1) WO1998041869A1 (fr)

Families Citing this family (47)

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US20030215798A1 (en) * 1997-06-16 2003-11-20 Diversa Corporation High throughput fluorescence-based screening for novel enzymes
ATE374815T1 (de) 1997-07-07 2007-10-15 Medical Res Council Methode zur erhöhung der konzentration von nucleinsäuremolekülen
GB9900298D0 (en) * 1999-01-07 1999-02-24 Medical Res Council Optical sorting method
IT1318605B1 (it) * 2000-06-30 2003-08-27 Uni Degli Studi Di Modena E Re Procedimoento per il dosaggio di tossine dsp del gruppo delledinophysitossine e delle yessottossine.
DK1356037T3 (da) 2001-01-25 2011-06-27 Evolva Ltd Bibliotek med en samling af celler
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
GB0127564D0 (en) 2001-11-16 2002-01-09 Medical Res Council Emulsion compositions
FR2842747B1 (fr) * 2002-07-23 2004-10-15 Commissariat Energie Atomique Procede et dispositif pour le criblage de molecules dans des cellules
CA2513899C (fr) 2003-01-29 2013-03-26 454 Corporation Procede d'amplification et de sequencage d'acides nucleiques
US10533998B2 (en) 2008-07-18 2020-01-14 Bio-Rad Laboratories, Inc. Enzyme quantification
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
US20060078893A1 (en) 2004-10-12 2006-04-13 Medical Research Council Compartmentalised combinatorial chemistry by microfluidic control
GB0307403D0 (en) 2003-03-31 2003-05-07 Medical Res Council Selection by compartmentalised screening
GB0307428D0 (en) 2003-03-31 2003-05-07 Medical Res Council Compartmentalised combinatorial chemistry
WO2005010145A2 (fr) 2003-07-05 2005-02-03 The Johns Hopkins University Procede et compositions de detection et d'enumeration de variations genetiques
US7459294B2 (en) 2003-08-08 2008-12-02 Kosan Biosciences Incorporated Method of producing a compound by fermentation
US20050221339A1 (en) 2004-03-31 2005-10-06 Medical Research Council Harvard University Compartmentalised screening by microfluidic control
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
AU2006335290A1 (en) 2006-01-11 2007-07-19 Raindance Technologies, Inc. Microfluidic devices and methods of use in the formation and control of nanoreactors
EP4190448A3 (fr) 2006-05-11 2023-09-20 Bio-Rad Laboratories, Inc. Dispositifs microfluidiques
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP3536396B1 (fr) 2006-08-07 2022-03-30 The President and Fellows of Harvard College Tensioactifs fluorocarbonés stabilisateurs d'émulsions
US8772046B2 (en) 2007-02-06 2014-07-08 Brandeis University Manipulation of fluids and reactions in microfluidic systems
WO2008130623A1 (fr) 2007-04-19 2008-10-30 Brandeis University Manipulation de fluides, composants fluidiques et réactions dans des systèmes microfluidiques
US8759020B2 (en) * 2008-07-02 2014-06-24 The University Of Wyoming Methods for natural product optimization
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
EP4047367A1 (fr) 2008-07-18 2022-08-24 Bio-Rad Laboratories, Inc. Procedé de détection d'analytes cibles au moyens des bibliothèques de gouttelettes
WO2010111231A1 (fr) 2009-03-23 2010-09-30 Raindance Technologies, Inc. Manipulation de gouttelettes microfluidiques
EP2486409A1 (fr) 2009-10-09 2012-08-15 Universite De Strasbourg Nanomatériau marqué à base de silice à propriétés améliorées et ses utilisations
WO2011079176A2 (fr) 2009-12-23 2011-06-30 Raindance Technologies, Inc. Systèmes microfluidiques et procédés pour réduire l'échange de molécules entre des gouttelettes
US9366632B2 (en) 2010-02-12 2016-06-14 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US10351905B2 (en) 2010-02-12 2019-07-16 Bio-Rad Laboratories, Inc. Digital analyte analysis
EP3392349A1 (fr) 2010-02-12 2018-10-24 Raindance Technologies, Inc. Analyse numérique d'analytes
EP2622103B2 (fr) 2010-09-30 2022-11-16 Bio-Rad Laboratories, Inc. Dosages sandwich dans des gouttelettes
WO2012103516A1 (fr) * 2011-01-28 2012-08-02 Amyris, Inc. Criblage de micro-colonies encapsulées dans du gel
EP3859011A1 (fr) 2011-02-11 2021-08-04 Bio-Rad Laboratories, Inc. Procédés permettant de former des gouttelettes mélangées
EP2675819B1 (fr) 2011-02-18 2020-04-08 Bio-Rad Laboratories, Inc. Compositions et méthodes de marquage moléculaire
US8841071B2 (en) 2011-06-02 2014-09-23 Raindance Technologies, Inc. Sample multiplexing
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
ES2527256B1 (es) * 2013-06-20 2015-11-10 Biomedal S.L. Determinación rapida de susceptibilidad a compuestos antimicrobianos
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US11193176B2 (en) 2013-12-31 2021-12-07 Bio-Rad Laboratories, Inc. Method for detecting and quantifying latent retroviral RNA species
US10647981B1 (en) 2015-09-08 2020-05-12 Bio-Rad Laboratories, Inc. Nucleic acid library generation methods and compositions
EP3864199A4 (fr) * 2018-10-08 2023-05-03 Amberstone Biosciences, Inc. Dosages compartimentés de produits biologiques bispécifiques et multispécifiques
WO2025046952A1 (fr) * 2023-08-29 2025-03-06 国立大学法人広島大学 Procédé et solution de criblage

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4401755A (en) * 1981-01-29 1983-08-30 Massachusetts Institute Of Technology Process for measuring microbiologically active material
US4399219A (en) * 1981-01-29 1983-08-16 Massachusetts Institute Of Technology Process for isolating microbiologically active material
SE441009B (sv) * 1982-03-08 1985-09-02 Kjell Nilsson Sett att immobilisera levande biomaterial i perlformiga polymerer
CA1196862A (fr) * 1983-06-01 1985-11-19 Anthony M.F. Sun Microencapsulement de cellules et de tissus vivants
SE8405105L (sv) * 1984-05-24 1985-11-25 Damon Biotech Inc Sett for screening av celler
US4801529A (en) * 1985-06-18 1989-01-31 Brandeis University Methods for isolating mutant microoganisms using microcapsules coated with indicator material
DE3871176D1 (de) * 1987-02-25 1992-06-25 Hoechst Ag Mikroenkapsulierung von biologisch aktivem material.
WO1989010786A2 (fr) * 1988-04-22 1989-11-16 Microdrop, Inc. Procede de formation et d'utilisation de microgouttelettes
GB9310557D0 (en) * 1993-05-21 1993-07-07 Smithkline Beecham Plc Novel process and apparatus
HUP9801871A3 (en) * 1995-04-24 1999-12-28 Chromaxome Corp San Diego Methods for generating and screening novel metabolic pathways

Also Published As

Publication number Publication date
WO1998041869A1 (fr) 1998-09-24
AU6571598A (en) 1998-10-12
EP0975969A4 (fr) 2002-07-31
EP0975969A1 (fr) 2000-02-02

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Legal Events

Date Code Title Description
FZDE Discontinued
FZDE Discontinued

Effective date: 20040318