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CA2277165A1 - Purification and/or concentration of dna by cross-flow filtration, separation of endotoxins from a nucleic acid preparation - Google Patents

Purification and/or concentration of dna by cross-flow filtration, separation of endotoxins from a nucleic acid preparation Download PDF

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Publication number
CA2277165A1
CA2277165A1 CA002277165A CA2277165A CA2277165A1 CA 2277165 A1 CA2277165 A1 CA 2277165A1 CA 002277165 A CA002277165 A CA 002277165A CA 2277165 A CA2277165 A CA 2277165A CA 2277165 A1 CA2277165 A1 CA 2277165A1
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CA
Canada
Prior art keywords
nucleic acid
cross
endotoxins
flow filtration
solution
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002277165A
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French (fr)
Other versions
CA2277165C (en
Inventor
Wolfgang Kuhne
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
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Individual
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Filing date
Publication date
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Publication of CA2277165A1 publication Critical patent/CA2277165A1/en
Application granted granted Critical
Publication of CA2277165C publication Critical patent/CA2277165C/en
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Saccharide Compounds (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Treatment Of Liquids With Adsorbents In General (AREA)

Abstract

The invention concerns a method of purifying and/or concentrating nucleic acids in a solution, the solution containing nucleic acid being guided tangentially past one or a plurality of semipermable membranes, such that the nucleic acid molecules are retained by the membranes and substances having a lower molecular weight can pass through the membranes and/or be adsorbed thereat, so that a purified and/or concentrated nucleic acid solution is obtained. The same method is carried out to separate endotoxins from a nucleic acid preparation.
The invention further concerns the use of a cross-flow filtration system for purifying and/or concentrating nucleic acids in a solution and for separating endotoxins from a nucleic acid preparation. The invention also concerns the use of the nucleic acids purified and/or concentrated by the cross-flow filtration system for cloning, transformation, transfection and microinjection into cells, for use in gene therapy processes, DNA vaccination and/or for polymerase chain reaction (PCR).
CA002277165A 1997-01-10 1998-01-09 Purification and/or concentration of dna by cross-flow filtration, separation of endotoxins from a nucleic acid preparation Expired - Fee Related CA2277165C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP97100331A EP0853123A1 (en) 1997-01-10 1997-01-10 Purification of DNA by 'cross-flow-filtration'
EP97100331.4 1997-01-10
PCT/EP1998/000103 WO1998030685A2 (en) 1997-01-10 1998-01-09 Purification and/or concentration of dna by cross-flow filtration, separation of endotoxins from a nucleic acid preparation

Publications (2)

Publication Number Publication Date
CA2277165A1 true CA2277165A1 (en) 1998-07-16
CA2277165C CA2277165C (en) 2004-03-30

Family

ID=8226362

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002277165A Expired - Fee Related CA2277165C (en) 1997-01-10 1998-01-09 Purification and/or concentration of dna by cross-flow filtration, separation of endotoxins from a nucleic acid preparation

Country Status (8)

Country Link
EP (2) EP0853123A1 (en)
JP (1) JP2000513580A (en)
KR (1) KR100322398B1 (en)
CN (1) CN1243543A (en)
BR (1) BR9807062A (en)
CA (1) CA2277165C (en)
TR (1) TR199901840T2 (en)
WO (1) WO1998030685A2 (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK201370621A1 (en) * 2013-10-25 2015-05-04 Amphi Consult V Martin Hesselsøe Aps A system and a method for concentrating traces of tissue from aquatic organisms in a water sample and use thereof
WO2016193206A1 (en) * 2015-05-29 2016-12-08 Curevac Ag A method for producing and purifying rna, comprising at least one step of tangential flow filtration
US11661634B2 (en) 2015-05-08 2023-05-30 CureVac Manufacturing GmbH Method for producing RNA

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB9425138D0 (en) 1994-12-12 1995-02-08 Dynal As Isolation of nucleic acid
US7807822B2 (en) 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
US6914137B2 (en) 1997-12-06 2005-07-05 Dna Research Innovations Limited Isolation of nucleic acids
GB2337261B (en) * 1998-05-15 2002-09-18 Fsm Technologies Ltd Separation of nucleic acids
DE19900681B4 (en) 1999-01-04 2007-04-12 Sartorius Ag Use of anion exchange membranes to remove endotoxins from nucleic acid containing solutions
ATE264386T1 (en) * 1999-07-23 2004-04-15 Genentech Inc METHOD FOR RNASE AND ORGANIC SOLVENT-FREE PURIFICATION OF PLASMID DNA BY TANGENTIAL FLOW FILTRATION
US6284117B1 (en) * 1999-12-22 2001-09-04 Nanogen, Inc. Apparatus and method for removing small molecules and ions from low volume biological samples
DE10006591B4 (en) * 2000-02-11 2007-03-29 Eppendorf Ag Process for the purification of nucleic acids
DE10022258A1 (en) * 2000-05-08 2001-11-15 Bayer Ag Purification and/or concentration of protein inclusion bodies in solution, by passing the solution tangentially across semipermeable membrane formed from intersecting longitudinal and transverse fibers
JP2003535589A (en) * 2000-06-02 2003-12-02 パル・コーポレーション Processing of plasmid-containing solutions
KR100356738B1 (en) * 2000-09-07 2002-10-18 주식회사 삼양제넥스 A method for removing endotoxin from the samples containing basic protein
GB0028361D0 (en) * 2000-11-21 2001-01-03 Glaxo Group Ltd Method of separating extra chromosomal dna from other cellular components
AU2003202026A1 (en) 2002-01-16 2003-09-02 Dynal Biotech Asa Method for isolating nucleic acids and protein from a single sample
JP2005538717A (en) 2002-09-13 2005-12-22 ヴァレンティス，インコーポレイテッド Apparatus and method for preparative scale purification of nucleic acids
US20060134620A1 (en) * 2002-11-28 2006-06-22 Mitsuharu Hirai Method and apparatus for concentration and purification of nucleic acid
TWI303275B (en) 2003-05-30 2008-11-21 Advisys Inc Devices and methods for biomaterial production
US8075780B2 (en) * 2003-11-24 2011-12-13 Millipore Corporation Purification and concentration of synthetic biological molecules
US8313906B2 (en) 2008-07-18 2012-11-20 Canon U.S. Life Sciences, Inc. Methods and systems for microfluidic DNA sample preparation
EP2506857B1 (en) 2009-12-01 2018-02-14 Translate Bio, Inc. Delivery of mrna for the augmentation of proteins and enzymes in human genetic diseases
AU2012267531B2 (en) 2011-06-08 2017-06-22 Translate Bio, Inc. Lipid nanoparticle compositions and methods for mRNA delivery
US20150267192A1 (en) 2012-06-08 2015-09-24 Shire Human Genetic Therapies, Inc. Nuclease resistant polynucleotides and uses thereof
EA202190410A1 (en) 2013-03-14 2022-03-31 Шир Хьюман Дженетик Терапис, Инк. CFTR mRNA COMPOSITIONS AND RELATED METHODS AND USES
ES2981185T3 (en) * 2013-03-14 2024-10-07 Translate Bio Inc Methods for purifying messenger RNA
WO2014140211A1 (en) 2013-03-15 2014-09-18 Novartis Ag Rna purification methods
CN103173438B (en) * 2013-04-10 2015-03-04 杭州百迈生物技术有限公司 Reagent and method for extracting plasmids
CA2928188A1 (en) 2013-10-22 2015-04-30 Shire Human Genetic Therapies, Inc. Mrna therapy for argininosuccinate synthetase deficiency
EA034103B1 (en) 2013-10-22 2019-12-27 Транслейт Био, Инк. METHOD OF TREATING PHENYLKETONURIA USING mRNA
EP3113865A4 (en) * 2014-03-07 2017-11-01 Agency For Science, Technology And Research Apparatus and methods for fractionation of biological products
JP6571679B2 (en) 2014-04-25 2019-09-04 トランスレイト バイオ， インコーポレイテッド Method for purifying messenger RNA
EP3585417B1 (en) 2017-02-27 2023-02-22 Translate Bio, Inc. Method of making a codon-optimized cftr mrna
WO2018213476A1 (en) 2017-05-16 2018-11-22 Translate Bio, Inc. Treatment of cystic fibrosis by delivery of codon-optimized mrna encoding cftr
WO2020041793A1 (en) 2018-08-24 2020-02-27 Translate Bio, Inc. Methods for purification of messenger rna

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2705251B1 (en) * 1993-05-13 1995-07-07 France Etat Armement Determination of substances in a liquid medium by tangential filtration.
EP0771355B1 (en) * 1994-07-15 2004-09-22 Merck & Co. Inc. A method for large scale plasmid purification
US6011148A (en) * 1996-08-01 2000-01-04 Megabios Corporation Methods for purifying nucleic acids

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK201370621A1 (en) * 2013-10-25 2015-05-04 Amphi Consult V Martin Hesselsøe Aps A system and a method for concentrating traces of tissue from aquatic organisms in a water sample and use thereof
DK178664B1 (en) * 2013-10-25 2016-10-24 Amphi Consult V Martin Hesselsøe Aps A system and a method for concentrating traces of tissue from aquatic organisms in a water sample and use thereof
US11661634B2 (en) 2015-05-08 2023-05-30 CureVac Manufacturing GmbH Method for producing RNA
US11274293B2 (en) 2015-05-29 2022-03-15 Curevac Real Estate Gmbh Method for producing and purifying RNA, comprising at least one step of tangential flow filtration
US10760070B2 (en) 2015-05-29 2020-09-01 Curevac Real Estate Gmbh Method for producing and purifying RNA, comprising at least one step of tangential flow filtration
EP3744843A1 (en) * 2015-05-29 2020-12-02 CureVac Real Estate GmbH A method for producing and purifying rna, comprising at least one step of tangential flow filtration
CN107873055A (en) * 2015-05-29 2018-04-03 库瑞瓦格股份公司 The method of generation and purifying RNA including at least one tangential flow filtration step
EP4108769A1 (en) * 2015-05-29 2022-12-28 CureVac Real Estate GmbH A method for producing and purifying rna, comprising at least one step of tangential flow filtration
WO2016193206A1 (en) * 2015-05-29 2016-12-08 Curevac Ag A method for producing and purifying rna, comprising at least one step of tangential flow filtration
US11667910B2 (en) 2015-05-29 2023-06-06 CureVac Manufacturing GmbH Method for producing and purifying RNA, comprising at least one step of tangential flow filtration
EP4108769B1 (en) 2015-05-29 2023-08-30 CureVac Manufacturing GmbH A method for producing and purifying rna, comprising at least one step of tangential flow filtration
US11760992B2 (en) 2015-05-29 2023-09-19 CureVac Manufacturing GmbH Method for producing and purifying RNA, comprising at least one step of tangential flow filtration
US11834651B2 (en) 2015-05-29 2023-12-05 CureVac Manufacturing GmbH Method for producing and purifying RNA, comprising at least one step of tangential flow filtration

Also Published As

Publication number Publication date
JP2000513580A (en) 2000-10-17
KR100322398B1 (en) 2002-03-18
WO1998030685A3 (en) 1998-10-22
CN1243543A (en) 2000-02-02
AU6208498A (en) 1998-08-03
CA2277165C (en) 2004-03-30
WO1998030685A2 (en) 1998-07-16
BR9807062A (en) 2000-05-02
TR199901840T2 (en) 2000-02-21
EP0853123A1 (en) 1998-07-15
AU723817B2 (en) 2000-09-07
EP0961826A2 (en) 1999-12-08
KR20000069943A (en) 2000-11-25

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