CA2260095C - Procede et dispositif pour mettre en oeuvre des tests quantitatifs d'affinite a fluorescence - Google Patents
Procede et dispositif pour mettre en oeuvre des tests quantitatifs d'affinite a fluorescence Download PDFInfo
- Publication number
- CA2260095C CA2260095C CA 2260095 CA2260095A CA2260095C CA 2260095 C CA2260095 C CA 2260095C CA 2260095 CA2260095 CA 2260095 CA 2260095 A CA2260095 A CA 2260095A CA 2260095 C CA2260095 C CA 2260095C
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- light
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Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/648—Specially adapted constructive features of fluorimeters using evanescent coupling or surface plasmon coupling for the excitation of fluorescence
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54366—Apparatus specially adapted for solid-phase testing
- G01N33/54373—Apparatus specially adapted for solid-phase testing involving physiochemical end-point determination, e.g. wave-guides, FETS, gratings
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- Health & Medical Sciences (AREA)
- Immunology (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Physics & Mathematics (AREA)
- General Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Pathology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Biomedical Technology (AREA)
- Hematology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Optics & Photonics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biotechnology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
Abstract
L'invention concerne un procédé et un dispositif pour mettre en oeuvre des tests immunologiques à fluorescence, notamment quantitatifs, par excitation de champs évanescents, éventuellement sur la base des différents essais biochimiques connus constitués en général de systèmes de récepteurs et de ligands. Toutefois, on évalue de préférence des systèmes d'anticorps et d'antigènes. L'invention permet d'effectuer des tests immunologiques quantitatifs à fluorescence avec différents essais biochimiques connus au moyen d'un dispositif très simple. On utilise à cet effet au moins une source de lumière (7, 8') qui émet des rayons d'une lumière pratiquement monochromatique d'une longueur d'onde qui provoque la fluorescence d'un indicateur lié à l'anticorps. Les rayons de lumière sont dirigés avec un angle alpha déterminé par une profondeur de pénétration prédéterminée pour le champ évanescent sur une surface limite (20) entre une plaque de base (1) optiquement transparente en un matériau dont l'indice de réfraction n1 est supérieur à l'indice de réfraction n2 du matériau au-dessus de la surface limite (20) et une zone en forme de cuvette (2) pour recevoir l'échantillon. La zone (2) est recouverte de son côté opposé à la plaque de base (1) par une plaque de couverture (3) et un détecteur (5) de lumière fluorescente est monté du même côté de la plaque de base (1) que la source de lumière (7).
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19628002.8 | 1996-07-11 | ||
| DE19628002A DE19628002C1 (de) | 1996-07-11 | 1996-07-11 | Vorrichtung und Verfahren zur Durchführung von Fluoreszenzimmunotests |
| PCT/DE1997/001499 WO1998002732A1 (fr) | 1996-07-11 | 1997-07-11 | Procede et dispositif pour mettre en oeuvre des tests quantitatifs d'affinite a fluorescence |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CA2260095A1 CA2260095A1 (fr) | 1998-01-22 |
| CA2260095C true CA2260095C (fr) | 2006-09-26 |
Family
ID=37057092
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA 2260095 Expired - Fee Related CA2260095C (fr) | 1996-07-11 | 1997-07-11 | Procede et dispositif pour mettre en oeuvre des tests quantitatifs d'affinite a fluorescence |
Country Status (1)
| Country | Link |
|---|---|
| CA (1) | CA2260095C (fr) |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108828206A (zh) * | 2018-05-24 | 2018-11-16 | 广州天宝颂原生物科技开发有限公司 | 一种干式荧光免疫分析仪及其测试方法 |
-
1997
- 1997-07-11 CA CA 2260095 patent/CA2260095C/fr not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| CA2260095A1 (fr) | 1998-01-22 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| EEER | Examination request | ||
| MKLA | Lapsed |
Effective date: 20160711 |
|
| MKLA | Lapsed |
Effective date: 20160711 |