CA2176615A1 - Culture vessel - Google Patents
Culture vesselInfo
- Publication number
- CA2176615A1 CA2176615A1 CA002176615A CA2176615A CA2176615A1 CA 2176615 A1 CA2176615 A1 CA 2176615A1 CA 002176615 A CA002176615 A CA 002176615A CA 2176615 A CA2176615 A CA 2176615A CA 2176615 A1 CA2176615 A1 CA 2176615A1
- Authority
- CA
- Canada
- Prior art keywords
- culture
- container
- culture vessel
- side tube
- tissue
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Abandoned
Links
- 238000004891 communication Methods 0.000 claims abstract description 4
- 239000012528 membrane Substances 0.000 claims description 4
- 238000009630 liquid culture Methods 0.000 abstract description 11
- 238000011109 contamination Methods 0.000 abstract description 5
- 238000000338 in vitro Methods 0.000 abstract description 4
- 239000007787 solid Substances 0.000 abstract description 4
- 230000008929 regeneration Effects 0.000 abstract description 3
- 238000011069 regeneration method Methods 0.000 abstract description 3
- 239000007788 liquid Substances 0.000 description 16
- 239000001963 growth medium Substances 0.000 description 14
- 239000000463 material Substances 0.000 description 5
- 239000002609 medium Substances 0.000 description 5
- 238000004161 plant tissue culture Methods 0.000 description 5
- 239000000356 contaminant Substances 0.000 description 4
- 239000002131 composite material Substances 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 230000009972 noncorrosive effect Effects 0.000 description 3
- 239000010935 stainless steel Substances 0.000 description 3
- 229910001220 stainless steel Inorganic materials 0.000 description 3
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 238000012544 monitoring process Methods 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000011521 glass Substances 0.000 description 1
- 239000011491 glass wool Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 235000021231 nutrient uptake Nutrition 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
- A01G31/02—Special apparatus therefor
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/001—Culture apparatus for tissue culture
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/20—Reduction of greenhouse gas [GHG] emissions in agriculture, e.g. CO2
- Y02P60/21—Dinitrogen oxide [N2O], e.g. using aquaponics, hydroponics or efficiency measures
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Developmental Biology & Embryology (AREA)
- Environmental Sciences (AREA)
- Cell Biology (AREA)
- Botany (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
Currently it is known to use solid as well as liquid culture mediums for in-vitro regeneration and mass propagation of plantlets. The present invention provides a culture vessel having a transparent container having a releasably fitted cap, at least one side tube having a releasably fitted cap and being in full communication with the lower part of the container through at least one passage, wherein the side tube is disposed at an inclina-tion with respect to the wall of the container, and at least one float member disposed within the container and having a porous support sheet to support the tissue for culture. This allows the tissue culture without subjecting the tissue to contamination.
Description
- 1 - 21766~S
FIELD OF INVENTION
The present invention relates to a culture vessel for plant tissue culture and is particularly useful for in vitro restoration and mass propagation of plantlets.
PRIOR ART
It is presently known to the art to use solid as well as liquid culture mediums for such in-vitro regeneration and mass propagation of plantlets. However, it has been found that the use of liquid culture medium in conventional processes of in-vitro regeneration of plantlets is more effective since it facilitates more oxygen and nutrient uptake by the cells/tissues which improves production levels. Culture vessels known in the art are normally found to be more suitable for use in solid culture medium since this suffers from certain inherent limitations in their construction for having the advance of the use of liquid culture medium. It is essential that for plant tissue culture with liquid culture medium, the plant tissue during culture be free of any contamination either from the atmosphere or in improper handling of the tissue while carrying out various stages of the culture. Further, in use of liquid culture medium, it is necessary to periodically monitor the culture medium to ensure proper quantity of the liquid medium to facilitate proper uptake of the medium by the tissue for the purpose of its culture.
Moreover, to carry out the various stages of culture of tissue, the liquid culture media requires to be changed from one formulation to another during the culture process.
While changing the liquid culture media, it is necessary that the tissue is not subjected to contamination. The conventional culture vessels presently used in the art are not suitable for plant tissue culture using liquid culture medium.
21766/~
OBJECTS OF THE INVENTION
:; .
An object of the present invention is to provide a culture vessel for plant tissue culture which can be advantageously utilised in carrying out the different shapes of plant tissue culture with liquid culture medium by changing the medium in the same vessel and thereby reducing the chances of contamination.
, BRIEF DESCRIPTION OF THE INVENTION
According to the present invention, there is provided a culture vessel comprising :
a transparent container having a releasably fitted cap;
atleast one side tube having a releasably fitted cap and being in flow communication with the lower part of said container through atleast one passage;
said side tube disposed at an inclination with respect to the wall of said container;
atleast one float member disposed within said container and having a porous support sheet to support the tissue for culture.
The culture vessel of the present invention allows a tissue culture without subjecting the tissue to contamination.
The releasable cap of the container restricts inflow of contaminants within the vessel during culture. Further, the side tube provides for necessary monitoring change of liquid culture media and/or maintaining particular predetermined level of the liquid media in the vessel and drain in/out to and from the vessel as and when required.
The requirement of the tissue to be transferred from one ~ - 3 ~ 21766/~
vessel to the other for subjecting the same to the culture stages is also carried out in the same vessel by introducing the appropriate liquid medium through the side tube into the vessel at each stage of culture. The side tube is also provided with a releasable cap at its open end to restrict inflow of any contaminants within the vessel through the tube.
DESCRIPTION WITH REFERENCE TO THE ACCOMPANYING DRAWINGS
Fig.1 is a schematic representation of the culture vessel according to the present invention showing the vessel fitted with the cap and housing the float member with the supported porous sheet inside the float;
Fig.2 is a sectional view of the float member illustrating the provision of the flange near its lower end to support the porous sheet; and Fig.3 is a further sectional view of the complete culture vessel along line AA' of Fig.1 with the cap fitted thereon.
Reference is made to Fig.1 wherein the culture vessel (V) comprises a container (CO) which may be cylindrical or of any other shape having a screw cap (Ca). The container (CO) is provided with side tube (ST) disposed at an inclination with respect to the side wall (WS) of container (CO). Side tube (ST) has a cap (Cp) removably held thereto.
As shown in Fig.3, cap (Cp) has a membrane made of any suitable resilient or puncturable material so as to allow an introduction or withdrawal of a needle of a syringe.
The side tube (ST) communicates with the inside of the container (CO) through passages (01) and (02) provided in side wall (WS). Passages (01) and (02) are disposed in a spaced relationship to each other, and such that passage (01) allows an introduction, addition or withdrawl of the culture medium into container (CO) without the `,~ 21766'~
removal of the cap (Ca). Passage (02) is disposed at a height above passage (01) and breaks the vacuum within side tube (ST). The container (C0) has a float member (F) which may also be cylindrically shaped or any other shape.
The shape of container (C0) and float member (F) is not of importance. Float (F) has a porous sheet (PS). In accordance with one e~bodiment, float (F) is provided with a radially inwardly extending flange (FL), as shown in Fig.2, which has a porous sheet (PS) held thereto.
Alternatively, as shown in Fig.3, flange (FL) supports porous sheet (PS). The tissue is placed on porous sheet (PS), which simultaneously allows absorption of the nutrient ;~
from the liquid media by the tissue. Thus, the tissue for culture is placed on the porous sheet (PS) and the float (F) is then introduced through the open end of container (C0). Thereafter, cap (Ca) closes container (C0) so as to provide a contaminant free atmosphere to the tissue.
The fitting of cap (Ca) to container (C0) can be effected either by means of threads which may be continuous or discontinous or any press fit connection of the cap (Ca) to coptainer (C0). The liquid media required for the tissue culture is fed into container (C0) through side tube (ST) and passage (01). As soon as the liquid media occupies container (C0) under float (F), the latter rises up and floats over the liquid media. The level of the liquid media is maintained up to a maximum upper limit so that it is lower than the height of second passage (02). The transparency of container (C0) and side tube (ST) further facilitates constant monitoring of the level of the liquid media in vessel (C0). During tissue culture, the liquid media is uptaken by the tissue and due to such uptake of the liquid medium, the level of the liquid starts falling.
Such fall in level of the liquid media in vessel (C0) is noted and the level is maintained by introduction of fresh liquid media through side tube (ST). Likewise, while carrying out various stages of the culture process, lf , ~ 21766~
it is essential to change the liquid media, side tube (ST) can be effectively utilised to drain out and/or introduce the liquid media necessary for the growth of plant tissue.
Side tube (ST) is provided with a releasably fitted cap (Cp). The cap (Cp) in accordance with a preferred embodiment, has a membrane (M) of rubber or any puncturable material to facilitate the draining of the introduction of the culture media to the container (CO) through the side tube (ST). The releasably fitted cap (Cp) can have a threaded connection to the side tube or may be press fitted onto the side tube's top end. Once the tissue is supported on sheet (PS), and in order to maintain the contAm;nAnt free atmosphere within container (CO), the introduction, replenishment or withdrawal of a culture medium is effected by puncturing membrane (M) with the needle of a syringe.
As shown in Figs. 2 & 3, float (F) comprises an inner wall (F), spaced from the outer wall (F2) so as to provide the required strength to float (F).
The threaded press fit connection of caps (Ca) and (Cp) may have continuous and/or discontinous threads to prevent ingress of contAminAnts into culture vessel (CO).
Preferably, cap (Ca) is provided with a radially inwardly and downwardly extending sloped bottom surface ~SL) as illustrated in Fig.3. The inclined sloped surface cap prevents cont~r;nAtion resulting from arrested condensed droplets held along the cap wall joint and/or its dripping down along the wall of the container.
It is obvious to mention that the culture vessel and its side tube of the invention which is shown to be cylindrical shaped as described above with reference to the accompanying figures of the preferred embodiments can be of any other ~ 21766~5 shape and configuration, triangular, circular, square, rectangular, oval and polygonal. Also, the shape of the float member and the perforated support structure (PS) can also vary depending upon such shape and configuration of the container. The container and the side tube can be selectively obtained or autoclavable/sterilisable, transparent, semi-transparent, non-corrosive metallic/non-metallic composite material such as glass, polymeric materials and stainless steel. The float can have side walls either solid or hollow and can be obtained of non-corrosive, metallic/non-metallic composite materials such as stainless steel and polymeric materials. The central support structure can be perforated/porous/woven mouled and can be selectively obtained of non-corrosive metallic/non-metallic composite material such as glass wool, polymeric materials or stainless steel. The support structure can be integral to the float matrix or may be an independent structure adapted tobe supported on the radially inwardly extended flange of the float and/or like adaption provided in the float member.
Further, it is possible to make obvious modification of the culture vessel of the invention described above and the scope of the invention should be determined keeping in view of the possibility of the obvious modifications of the disclosure according to the present invention. It may be noted in this respect that the culture vessel of the invention can have more than one side tube instead of the one side tube described in the illustrated preferred embodiments.
FIELD OF INVENTION
The present invention relates to a culture vessel for plant tissue culture and is particularly useful for in vitro restoration and mass propagation of plantlets.
PRIOR ART
It is presently known to the art to use solid as well as liquid culture mediums for such in-vitro regeneration and mass propagation of plantlets. However, it has been found that the use of liquid culture medium in conventional processes of in-vitro regeneration of plantlets is more effective since it facilitates more oxygen and nutrient uptake by the cells/tissues which improves production levels. Culture vessels known in the art are normally found to be more suitable for use in solid culture medium since this suffers from certain inherent limitations in their construction for having the advance of the use of liquid culture medium. It is essential that for plant tissue culture with liquid culture medium, the plant tissue during culture be free of any contamination either from the atmosphere or in improper handling of the tissue while carrying out various stages of the culture. Further, in use of liquid culture medium, it is necessary to periodically monitor the culture medium to ensure proper quantity of the liquid medium to facilitate proper uptake of the medium by the tissue for the purpose of its culture.
Moreover, to carry out the various stages of culture of tissue, the liquid culture media requires to be changed from one formulation to another during the culture process.
While changing the liquid culture media, it is necessary that the tissue is not subjected to contamination. The conventional culture vessels presently used in the art are not suitable for plant tissue culture using liquid culture medium.
21766/~
OBJECTS OF THE INVENTION
:; .
An object of the present invention is to provide a culture vessel for plant tissue culture which can be advantageously utilised in carrying out the different shapes of plant tissue culture with liquid culture medium by changing the medium in the same vessel and thereby reducing the chances of contamination.
, BRIEF DESCRIPTION OF THE INVENTION
According to the present invention, there is provided a culture vessel comprising :
a transparent container having a releasably fitted cap;
atleast one side tube having a releasably fitted cap and being in flow communication with the lower part of said container through atleast one passage;
said side tube disposed at an inclination with respect to the wall of said container;
atleast one float member disposed within said container and having a porous support sheet to support the tissue for culture.
The culture vessel of the present invention allows a tissue culture without subjecting the tissue to contamination.
The releasable cap of the container restricts inflow of contaminants within the vessel during culture. Further, the side tube provides for necessary monitoring change of liquid culture media and/or maintaining particular predetermined level of the liquid media in the vessel and drain in/out to and from the vessel as and when required.
The requirement of the tissue to be transferred from one ~ - 3 ~ 21766/~
vessel to the other for subjecting the same to the culture stages is also carried out in the same vessel by introducing the appropriate liquid medium through the side tube into the vessel at each stage of culture. The side tube is also provided with a releasable cap at its open end to restrict inflow of any contaminants within the vessel through the tube.
DESCRIPTION WITH REFERENCE TO THE ACCOMPANYING DRAWINGS
Fig.1 is a schematic representation of the culture vessel according to the present invention showing the vessel fitted with the cap and housing the float member with the supported porous sheet inside the float;
Fig.2 is a sectional view of the float member illustrating the provision of the flange near its lower end to support the porous sheet; and Fig.3 is a further sectional view of the complete culture vessel along line AA' of Fig.1 with the cap fitted thereon.
Reference is made to Fig.1 wherein the culture vessel (V) comprises a container (CO) which may be cylindrical or of any other shape having a screw cap (Ca). The container (CO) is provided with side tube (ST) disposed at an inclination with respect to the side wall (WS) of container (CO). Side tube (ST) has a cap (Cp) removably held thereto.
As shown in Fig.3, cap (Cp) has a membrane made of any suitable resilient or puncturable material so as to allow an introduction or withdrawal of a needle of a syringe.
The side tube (ST) communicates with the inside of the container (CO) through passages (01) and (02) provided in side wall (WS). Passages (01) and (02) are disposed in a spaced relationship to each other, and such that passage (01) allows an introduction, addition or withdrawl of the culture medium into container (CO) without the `,~ 21766'~
removal of the cap (Ca). Passage (02) is disposed at a height above passage (01) and breaks the vacuum within side tube (ST). The container (C0) has a float member (F) which may also be cylindrically shaped or any other shape.
The shape of container (C0) and float member (F) is not of importance. Float (F) has a porous sheet (PS). In accordance with one e~bodiment, float (F) is provided with a radially inwardly extending flange (FL), as shown in Fig.2, which has a porous sheet (PS) held thereto.
Alternatively, as shown in Fig.3, flange (FL) supports porous sheet (PS). The tissue is placed on porous sheet (PS), which simultaneously allows absorption of the nutrient ;~
from the liquid media by the tissue. Thus, the tissue for culture is placed on the porous sheet (PS) and the float (F) is then introduced through the open end of container (C0). Thereafter, cap (Ca) closes container (C0) so as to provide a contaminant free atmosphere to the tissue.
The fitting of cap (Ca) to container (C0) can be effected either by means of threads which may be continuous or discontinous or any press fit connection of the cap (Ca) to coptainer (C0). The liquid media required for the tissue culture is fed into container (C0) through side tube (ST) and passage (01). As soon as the liquid media occupies container (C0) under float (F), the latter rises up and floats over the liquid media. The level of the liquid media is maintained up to a maximum upper limit so that it is lower than the height of second passage (02). The transparency of container (C0) and side tube (ST) further facilitates constant monitoring of the level of the liquid media in vessel (C0). During tissue culture, the liquid media is uptaken by the tissue and due to such uptake of the liquid medium, the level of the liquid starts falling.
Such fall in level of the liquid media in vessel (C0) is noted and the level is maintained by introduction of fresh liquid media through side tube (ST). Likewise, while carrying out various stages of the culture process, lf , ~ 21766~
it is essential to change the liquid media, side tube (ST) can be effectively utilised to drain out and/or introduce the liquid media necessary for the growth of plant tissue.
Side tube (ST) is provided with a releasably fitted cap (Cp). The cap (Cp) in accordance with a preferred embodiment, has a membrane (M) of rubber or any puncturable material to facilitate the draining of the introduction of the culture media to the container (CO) through the side tube (ST). The releasably fitted cap (Cp) can have a threaded connection to the side tube or may be press fitted onto the side tube's top end. Once the tissue is supported on sheet (PS), and in order to maintain the contAm;nAnt free atmosphere within container (CO), the introduction, replenishment or withdrawal of a culture medium is effected by puncturing membrane (M) with the needle of a syringe.
As shown in Figs. 2 & 3, float (F) comprises an inner wall (F), spaced from the outer wall (F2) so as to provide the required strength to float (F).
The threaded press fit connection of caps (Ca) and (Cp) may have continuous and/or discontinous threads to prevent ingress of contAminAnts into culture vessel (CO).
Preferably, cap (Ca) is provided with a radially inwardly and downwardly extending sloped bottom surface ~SL) as illustrated in Fig.3. The inclined sloped surface cap prevents cont~r;nAtion resulting from arrested condensed droplets held along the cap wall joint and/or its dripping down along the wall of the container.
It is obvious to mention that the culture vessel and its side tube of the invention which is shown to be cylindrical shaped as described above with reference to the accompanying figures of the preferred embodiments can be of any other ~ 21766~5 shape and configuration, triangular, circular, square, rectangular, oval and polygonal. Also, the shape of the float member and the perforated support structure (PS) can also vary depending upon such shape and configuration of the container. The container and the side tube can be selectively obtained or autoclavable/sterilisable, transparent, semi-transparent, non-corrosive metallic/non-metallic composite material such as glass, polymeric materials and stainless steel. The float can have side walls either solid or hollow and can be obtained of non-corrosive, metallic/non-metallic composite materials such as stainless steel and polymeric materials. The central support structure can be perforated/porous/woven mouled and can be selectively obtained of non-corrosive metallic/non-metallic composite material such as glass wool, polymeric materials or stainless steel. The support structure can be integral to the float matrix or may be an independent structure adapted tobe supported on the radially inwardly extended flange of the float and/or like adaption provided in the float member.
Further, it is possible to make obvious modification of the culture vessel of the invention described above and the scope of the invention should be determined keeping in view of the possibility of the obvious modifications of the disclosure according to the present invention. It may be noted in this respect that the culture vessel of the invention can have more than one side tube instead of the one side tube described in the illustrated preferred embodiments.
Claims (10)
1. A culture vessel comprising :
a transparent container having a releasably fitted cap;
atleast one side tube having a releasably fitted cap and being in flow communication with the lower part of side container through atleast one passage;
a porous support sheet to support the tissue for culture.
a transparent container having a releasably fitted cap;
atleast one side tube having a releasably fitted cap and being in flow communication with the lower part of side container through atleast one passage;
a porous support sheet to support the tissue for culture.
2. A culture vessel as claimed in claim 1 wherein the side wall of said container has a first opening, a second opening spaced from and at a height above said second first opening, said openings being in flow communication with said side tube.
3. A culture vessel as claimed in claim 1 wherein said float member has a radially inwardly extended flange with a porous support sheet.
4. A culture vessel as claimed in claim 3 wherein said support sheet is supported on said flange.
5. A culture vessel as claimed in claim 3 wherein said support sheet is held to said flange.
6. A culture vessel as claimed in claim 1 wherein said float comprises one inner and outer wall, said inner wall spaced from the outer wall.
7. A culture vessel as claimed in claim 1 wherein the cap of said side tube has a membrane.
8. A culture vessel as claimed in claim 1 wherein the cap of said container has inwardly downward surface.
9. A culture vessel as claimed in any of claims 1-8 substantially as described hereinabove.
10. A culture vessel as claimed in any of claims 1-8 substantially as illustrated in any of the drawings.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| IN546CA1995 IN183604B (en) | 1995-05-16 | 1995-05-16 | |
| IN546/CAL/95 | 1995-05-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA2176615A1 true CA2176615A1 (en) | 1996-11-17 |
Family
ID=11083404
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA002176615A Abandoned CA2176615A1 (en) | 1995-05-16 | 1996-05-14 | Culture vessel |
Country Status (9)
| Country | Link |
|---|---|
| JP (1) | JP3035384U (en) |
| CN (1) | CN1138095A (en) |
| AU (1) | AU4443496A (en) |
| CA (1) | CA2176615A1 (en) |
| DE (1) | DE19619114A1 (en) |
| FR (1) | FR2734280A1 (en) |
| GB (1) | GB2301374A (en) |
| IN (1) | IN183604B (en) |
| NL (1) | NL1003139C1 (en) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2980867B2 (en) * | 1996-06-18 | 1999-11-22 | 明一 廖 | Cell culture method and culture device |
| DE19801763C2 (en) * | 1998-01-19 | 1999-10-28 | Ulrich Mohr | Culture device and method for culturing cells or tissue components |
| US6794184B1 (en) | 1998-01-19 | 2004-09-21 | Ulrich Mohr | Culturing device and method for culturing cells or tissue components |
| DE102004024834A1 (en) * | 2004-05-19 | 2006-01-12 | Universität Rostock | Apparatus for conducting a liquid-air culture of epithelium |
| DE102005002938B4 (en) * | 2004-11-08 | 2006-12-28 | Minuth, Will, Prof. Dr. | Method for supplying cells or tissues |
| CN100399883C (en) * | 2005-04-15 | 2008-07-09 | 中国科学院沈阳应用生态研究所 | Plant tissue culturing liquid container |
| DE102006043656B4 (en) | 2006-09-18 | 2023-08-10 | Pieter Van Weenen & Co. Gmbh The House Of Innovation | Gassing device and system |
| NL2012922B1 (en) * | 2014-05-30 | 2016-06-09 | Ccm Beheer Bv | Container for culturing organisms, method for monitoring the culturing of organisms inside said container, and monitoring system. |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4912058A (en) * | 1989-04-27 | 1990-03-27 | Becton, Dickinson And Company | Roller bottle |
-
1995
- 1995-05-16 IN IN546CA1995 patent/IN183604B/en unknown
-
1996
- 1996-02-09 AU AU44434/96A patent/AU4443496A/en not_active Abandoned
- 1996-05-10 GB GB9609762A patent/GB2301374A/en not_active Withdrawn
- 1996-05-11 DE DE19619114A patent/DE19619114A1/en not_active Withdrawn
- 1996-05-14 CA CA002176615A patent/CA2176615A1/en not_active Abandoned
- 1996-05-15 FR FR9606094A patent/FR2734280A1/en active Pending
- 1996-05-15 NL NL1003139A patent/NL1003139C1/en not_active IP Right Cessation
- 1996-05-15 CN CN96107443A patent/CN1138095A/en active Pending
- 1996-05-15 JP JP1996004151U patent/JP3035384U/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| CN1138095A (en) | 1996-12-18 |
| IN183604B (en) | 2000-03-04 |
| GB9609762D0 (en) | 1996-07-17 |
| JP3035384U (en) | 1997-03-18 |
| AU4443496A (en) | 1996-11-28 |
| NL1003139C1 (en) | 1996-11-19 |
| FR2734280A1 (en) | 1996-11-22 |
| DE19619114A1 (en) | 1996-12-05 |
| GB2301374A (en) | 1996-12-04 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US4296205A (en) | Cell culture and continuous dialysis flask and method | |
| CA1306714C (en) | Cell culture flask utilizing a membrane barrier | |
| US3448011A (en) | Sterile filter | |
| US7951555B2 (en) | Membrane bioreactor | |
| US5783075A (en) | Disposable dialyzer apparatus | |
| US4596779A (en) | Culture vessel with agitator | |
| CA2106918A1 (en) | Cell Culture Insert | |
| EP0311609A4 (en) | Shake flask having aeration enhancing means. | |
| GB2334965A (en) | Cell culture vessel | |
| CA2176615A1 (en) | Culture vessel | |
| US5702945A (en) | Culture vessel for cell cultures on a carrier | |
| IL104385A (en) | Method and apparatus for growing biomass particles | |
| US4921604A (en) | Upflow biological reator waste water treatment system | |
| US6123840A (en) | Organic waste water treating apparatus having treating layer and auxiliary layer | |
| US6210959B1 (en) | Apparatus for the cultivation and concentration of non-adherent cells as well as for co-cultivation of two different cell species | |
| KR930000263B1 (en) | Vapor-liquid contacting apparatus | |
| IL116365A (en) | Culture vessel | |
| AT504648B1 (en) | Bioreactor comprises a cylindrical sheath, an agitator, a cover, a dished base, valves present in the base, and an insert-part housing the agitator and engaged under formation of a circulating gutter | |
| JPH04158781A (en) | Culture device | |
| DE102004029709A1 (en) | Device for culturing cells in a vessel, useful e.g. for preparation of antibodies, comprising an oscillatory stirrer, membrane basket, containing carrier, and gasification membrane on the outside of the basket | |
| JP3780406B2 (en) | Methane fermentation equipment | |
| CN216063317U (en) | Laboratory vessel draining device | |
| JPH03240483A (en) | Method for gas/liquid contact and system therefor | |
| CN220696809U (en) | Device for placing sample in beaker | |
| US454586A (en) | Fermenting-vat |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FZDE | Discontinued |