CA2066945C - Oral, cutaneous and intravaginal pharmaceutical compositions in the form of foam - Google Patents

Abstract

A liquid pharmaceutical composition which can be administered as a foam by means of suitable supplying devices, without necessity of gas propeller, said composition consisting of:
a) one or more natural or synthetic ionic or non ionic, acid, basic or neutral surfactants;
b) a solvent or a solvent mixture;
c) an active ingredient or a combination of active ingredients;
d) optional adjuvants or excipients.

Description

ORAL, COTANEOBS AND INTRAVAGINAL PHARMACF.OTIC~ COP3P0-SITIONS IN THE FORM OF FOAM
The present invention relates to pharmaceutical compositions in the form of foam.
Topical administration of active ingredients ad vantageously allows the maximum concentration of the S drug directly near the biophase and contemporaneously avoids that the dispersion of the drug into tissues, which are not concerned with the presence of specific receptors or target organs, may cause unnecessary risks of toxicity or intolerance.
Recently, particular attention has been devoted to the development of compositions with high consistency, such as gel or ointments or liquid compositions to be respectively applied by means of special adapters, such as nasal inhalators or sprays, vaginal douches or cre-ams, etc. on the skin, or on the mucosae coating some body cavities.
Residence time of the composition at the site of application is critically affected by the consistency of the pharmaceutical composition, therefore a non op-timized vehicle can negatively affect the therapeutic efficacy.
The problem is particularly critical in the case of intravaginal administration, since a poorly viscous composition is immediately diluted and expelled, on the contrary, a composition with high consistency, such as a cream, prevents the diffusion of the active ingre-dient, thus limiting its activity to the site of depo-sition only. Antibacterial and antimycotic drugs are generally poorly absorbed and a blocked diffusion of the active ingredient gives rise to a partial therapeutic effect only. Further, a reduced drug diffusion may cause irritation and local side effect enhancement.
In the case of cutaneous administration, a high viscosity of the composition requires a stronger spreading, causing burning or pain if the tissues axe irritated or damaged;
On the contrary a non viscous composition can be fastly eliminated from the site of application.
In one embodiment the present invention provides a liquid pharmaceutical composition, which is propellant free and mechanically foamed, the composition consisting of a) a natural or synthetic, ionic or non ionic surfactant or a surfactant mixture, having acid, basic or neutral characteristics b) a solvent or a solvent mixture, preferably water or water/ethanol c) a natural or synthetic active ingredient d) optional adjuvants or excipients.
Compositions, which are similar to the above described ones, wherein the role of active ingredient is performed by surfactants and/or the solvents themselves, are a further object of the present invention. Such a composition can be useful in a topical intravaginal application when a therapeutic and/or hydrating, lubricating or protective effect is desired.
Conventional devices can be used to supply the compositions of the invention in the form of a foam.
Particularly preferred devices are the ones described in EP-A-336188, which consist of a mixer wherein the liquid and air flows, properly regulated by the dimen-sions of holes, are forced through said holes, which are free or provided with drawing tube, or similar ones.
For the cutaneous or intravaginal application, properly shaped cannulae can conveniently be inserted on the supplying spouts in order to facilitate the distribution and localization of the foam.
The surfactants according to the invention have the purpose to place themselves at the gas-liquid in terface, assisting the inclusion into the liquid of an air or gas mass which is sufficient to create the desi red foaminess. Among the synthetic surfactants, alkyla midobetaine, quaternary ammonium salts, poloxamers and the like are preferred, while among the natural ones, the phospholipids, and the like, are preferred. In any case, the percent content ranges from 0.01 to 20%.
The compositions of the invention can be admini stered not only by cutaneous and intravaginal route, but also by oral route.
Generally, the solvent of the composition is water or a water-ethanol mixture. Also other pharmaceutically acceptable solvents can be used.
Examples of active ingredients which can advanta geously be included in the compositions of the inven tion, optionally combined between them, are antimycotic (ciclopirox olamine), antiinflammatory (diclofenac, benzidamine, piroxicam, tiaprofen, ketoprofen, tetrida mine), corticosteroid (hydrocortisone and others), im munomodulator (Pidotimod and others), mucosecretolytic (sobrerol, carboxymethylcysteine), wound healing ~0~~~~a agents, vegetal extracts (Triticum vulgaris), amino acids, vitamins, xanthines (cyclopropylrnethylxantine).
The active ingredients are contained in the compo sitions of the invention in percent amounts which de pend from the nature of the active ingredient, but ge nerally range from 0.01°~ to 20% w/v.
The compositions further contain excipients, such as preservatives, stabilizers, thickening agents, gel-ling agents, flavouring agents, dyes, cosolvents, structuring agents, etc., which will be selected accor-ding to the administration route and the kind of active ingredient.
The advantages of the invention are illustrated by the Lollowing pharmacological and clinical tests.
The foam containing 2°,6 ciclopirox olamine (Example n. 1) for the intravaginal administration was tested in comparison with an aqueous composition containing the same active ingredient at the same concentration in the treatment of rat vaginal infection from Candida albi cans.
Sprague Dawley.(C. River)'female rats weighing 100 g were ovariectomized and hysterectomized under nembu-tal anesthesia (40 mg/kg/i.p.).
After about three weeks from the operation, the rats were administered subcutaneously with 100 )Zg/day of estradiol vehicled in sesam oil, in order to induce pseudo estruation, which was monitored by vaginal smear exam with microscopy.
The rats which showed pseudo estruation were in fected with 106 Candida albicans cells, diluted in 0.2 ml of saline.

The treatment with 2°~ ciclopirox olamine vaginal foam or aqueous solution started three days after the infection and lasted 10 days. From the fourth day on, a vaginal withdrawal with sterile cotton swab was perfor-med each morning. The swab was put into Sabouraud agar containing 20 TU/ml of penicillin C and 40 pg/ml of strepromycin for a microbiological test of the infec-tive state.
The results reported on Table 1 show that both the ciclopirox olamine preparations are active towards the experimental infections but that the vaginal foam is much more faster than the aqueous composition due to the above mentioned characteristics.
Table 1 Ciclopirox olamine vaginal foam curative activity in comparison with the aqueous composition against vaginal infection from Candida albicans in the rat.
Treatment Female N. of animals with negative microbiolo:
rats gical test in day N. 4° I 5° I G° I 7° I F3° ~ 9°
1 10°
Saline 1 20 ~ 0/2o I olzo I o/20 ~ o/zo I o/2o I o/20 ~ 0/20 2% Ciclopi=
rox olamine -.
vaginal f aam~ 20 10/20 19/20 18/20 20/20 20/20 20/20 20/20 2°~ Ciclopi=
rox olamine aqueous solution 20 5/20 7/20 10/20 13/20 17/20 20/20 20/20 The ciclopirox olamine vaginal foam formulation further proved to be actively tolerated in the one month toxicity tests performed an female rats and rab-bits.
In the case of cutaneous topical administration, surfactant high concentration and foam tenderness allow the medicament to be easily spreaded on the tissues and cutaneous annexes: foam breaking, which can be modula-ted according to the preparation parameters, allows spreading a homogenous and continuous solution film on the administration area without the necessity to force mechanically the dispersion of the medicament, con-trarily to what happens for highly structured vehicles.
The 2°~ tetridamine topical foam (Example 2) was tested in comparison with an aqueous solution contai-ning the same amount in the carrageen inflammation test and the analgesic activity test in the rat.
The antiinflammatory activity was determined on the carrageenin oedema (Winter et al, Proc. Soc. Exp.
Biol.Med, 111, 544, 1962); the analgesic activity was tested on the same rats by means of the Randall and Se-litto~s test (Arch. Int. Pharmacodyn. 111, 409, 1957).
Sprague bawley (C. River) male rats, weighing 200 g, were injected with 2°~ carrageenin saline (0.1 ml/rat) in the upper right pad and treated three times with the tetridamine topical foam or topical solution (3 times at 20 minutes intervals). The volume of the inflamed pad was measured before and after 1, 2, 3, 4 or 5 hours the carrageenin injection with a plethysmograph (Basile, Italy).

Table 2 Topical antiinflammatory activity of tetridamine topical foam in comparison with the aqueous solution in carrageenin oedema.
SITreatment Rats Volume difference of inflamed pad against basal (ml) N., 1h ~ 2h ~ 3h ~ 4h ~ 5h ~ Saline 10 0,48~0,02 0,6210,03 0,6910,04 0,6010,05 0,3810,02 2°/° Tetri=
~damine to~
10~ pical foam l0 0,3610,02* 0,2210,02* 0,2310,03* 0,24~0,03* 0,1810,02 ~ 2~° Tetri-~ damine ~ aqueous solution 10 0,3910,03 0,3010,03 0,4010,04 0,40~0,04 0,3010,02 *
P < 0,01 Dunnett's t vs.~ controls 15 P < 0,01 Stude:nt's t vs. aqueous solution Table 3 Topical analgesic activity of tetridamine topical foam in comparison with the aqueous solution (Randall and Selitto's test).

Treatment Rats Sensitivity pain difference to against basal (g) N. ' 1h 2h ' 3h 4h 5h Saline 10 85,618,16,7110,1 75,818,3 70,319,555,418,7 2% Tetri=

25 damine to-pic al foam10 27,814,5*25,6t3,2*26,7t4,5* 28,314,5*20,6t4,6*

2~ Tetri= ...

damine aqueous solution 10 36,716,139,714,3 40,615,2 50,616,243,216,7 P < 0,01 Dunnett's vs. controls t 30 P < 0,01Student's vs: aqueous solution t~

_..

The sensitivity to pain of the inflamed pad was measured in g of weight before and after 1, 2, 3, 4 and hours the carrageenin infection by means of a pain tester (Basile, Italy).
5 Table 2 shows that tetridamine topical foam has topical antiinflammatory activity higher and longer than the aqueous formulation one.
Similarly, Table 3 shows that the topical foam al lows to obtain a stronger .and longer analgesic activity in the rat. Those results are related to the foam com position which allows to obtain a better and longer ab-sorption of the active ingredient tetridamine.
The tetridamine topical foam proved to be actively tolerated in one-month toxicity tests.
In the case of oral administration, the appearance of the dose to be administered, if appropriately flavoured, can make it more agreeable by patients ha-ving difficult swallowing, such as elder people or children, thus complying with the prescribed posology.
Pidotimod oral foam (Example 4) was tested within two group of patients ~.n comparison with an aqueous , solution having the same concentration. The first. group included 40 children, male and female, aging between S
and 10; the second group included 40 elder people, male and female, aging between 65 and 80.
The patient could take one of the two compositions according to choice, twice a day (8.00 a.m. and 8.00 p.m.). A week after, each patient had to select the ~areferred composition.

~~~f~~~

Table 4 Preference of the two Pidotimod compositions by two po-pulations of patients.
Compositions Children Elders (n. of patients (n. of patients which preferred) which preferred) Pidotimod 35/40 28/40 oral foam Pidotimod 10aqueous solution 05/4p 12/40 Table 4 clearly shows that both populations of pa-tients mostly preferred the oral foam composition.
The following examples further illustrate the in-vention.
Ex~Pr~E 1 200 g of ciclopirox olamine were dissolved in the minimum quantity of propylene glycol, then 3.5 1 of an aqueous solution containing 0.2°,6 of trimethylacetyl am-monium p-toluenesulfonate and 3.5°~ of fatty acids diethanolamide were added.
The solution was completed by adding perfume, na tural vegetal extract and propylene glycol up to a 10 liter volume, adjusting p~T to physiological value with lactic acid.
fihe above solution, once put into the vessel equipped with the appropriate device, supplied a soft and persisting foam, which could be spreaded an the skin for dermal mycosis treatment, or directed into the vaginal cavity by means of a cannula.
The same result was achieved by using the above ~~~~~e~~~
to composition with a gaseous propellant at ordinary pressure or a liquid one at high pressure, such as isobutane in a normal pressurized bottle.
TV 'MITT T ~'.

10 g of tetridamine maleate, 30 g of alkylamidobe-taine, 1 g of sodium methyl-p-hydroxybenzoate were dis-solved in about 500 ml of deionized wa~.er. The clear solution was buffered at pH 4.5 with lactic acid and the volume was adjusted at 1 1. The solution was tran-sferred into bottles provided with supplying system and vaginal cannula to allow a foam having antiinflammatory and antiphlogistic properties to be supplied.
100 g of tiaprofenic acid were salified in stui-chiometric ratio with tromethamine in 500 g of an aque-ous solution containing the suitable amounts of antimi- , crobic agents.
100 g of alcohol and 100 g of a quaternary surfac-tant of the betaine type were then added.
The solution was buffered at pH about 7, filtered and put into the suitable containers equipped with foam supplying valve. The so produced foam could easily be spreaded on the skin near articulations in order to as sist the penetration of the active ingredient and to let the knoTan antiinflammatory activity begin.
Z~vravnTn a $0 g of Pidotimod were dissolved by salification with 40 g of tromethamine in 500 g of a solutian containing conventional sweeteners, antimicrobial agents, 150 g of sorbitol, 50 g of polyoxyethylene--polf~oxypropylene copolimer and a suitable amount of ~~~6~~~

flavouring agents. pH was adjusted to a slight acidic value and the final volume was adjusted to 1 1. The obtained solution was distributed into suitable dispensers equipped with foam generating valve. The foam, which was obtained with a simple manual pressure, was collected in a measure and used as vehicle for pediatric administration. In fact, the foam exhibited specific taste characteristics and a perfect edible property for the type of administrations wherein chewing or swallowing may be impaired. The same composition, put into an anodised aluminium gas bottle in the presence of a suitable quantity of propellant supplied from a normally used spray foam valve supplied a long lasting foam having a similar consistence.
ExAMpa.E S
A ethanolic solution containing 50 g of lecithin, 10 g of cholesterol and 20 g of ciclopirox olamine was mixed with 800 g of an al~Cylamidobetaine aqueous solu-tion, protected against bacterial pollution with a sui-table amount of sodium methyl-p-hydroxybenzoate and acidified with lactic acid.
The liposome suspension was then transferred into a bottle equipped with a foam generating valve and op-tionally equipped with a cannula for the endovaginal administration: A similar result was obtained transfer-ring the above aqueous solution and the alcoholic one into a gas bottle, which had been pressurized with a sufficient amount of isobutane: operating the supplying button of a normal aerosol valve, evaporation of propellant was thus caused, therefore the alcohol, being the more volatile component, caused the immediate 1z formation of a liposomial foam.
With a procedure similar to the one described in Example 2, tetridamine was substituted with 60 g' of Triticum vulgare exctract (dried residue equivalent to ?.00 mg/100 ml? obtaining a composition which, once put into a bottle equipped with a supplying valve could generate a foam which assisted the healing of the dermal tissues requiring reactivation.

With a procedure similar to the one described in Example 4, instead of Pidotimod, a pool of amino acids consisting of 6 g of L-phosphoserine, 15 g of L-argi-nine hydrochloride, 2 g of L-phosphothreonine, 7.5 g of L-glutamine and 50 mg of vitamine B-12 were used as active ingredient.
An edible foam having characteristic flavour and very good swallowability was obtained.

Claims (10)

1. A liquid pharmaceutical composition, which is propellant free and mechanically foamed, said composition consisting of:
a) one or more natural or synthetic ionic or non ionic, acid, basic or neutral surfactants selected from alkylamidobetaines, poloxamers, quaternary ammonium salts and phospholipids in amounts ranging from 0.01 to 20%;
b) water or water/ethanol;
c) an active ingredient or a combination of active ingredients.

2. A composition according to claim 1, which is contained in a dosing bottle equipped with a foam supplying valve, consisting of a microdrilled device to which the solution and part of the air or gas contained in the bottle itself arrive separately by means of manual action on the bottle.

3. A composition according to claim 1 or 2 wherein the active ingredient is selected from the group consisting of antimycotic, antiinflammatory, immunomodulating, mucosecretolytic, wound-healing, repairer, antiphlogistic, anticongestion agents, amino acids, vitamins, corticosteroids or a combination thereof.

4. A composition according to any one of claims 1 to 3 which is orally administrable.

5. A composition according to any one of claims 1 to 4 characterized in that the foam supplied from a valve-adaptor can be directed into a vaginal cavity by means of a vaginal cannula.

6. A composition according to any one of claims 1 to 5 characterized in that the active ingredient is ciclopirox olamine.

7. A composition according to any one of claims 1 to 5 characterized in that the active ingredient is selected from tetridamine base or a salt thereof, benzidamine base or a salt thereof, Pidotimod base or a salt or a derivative thereof, Triticum vulgare extract, hydrosoluble or lipsoluble vitamins or amino acids in combination and/or mineral salts, carboxymethylcysteine and/or the salts or derivatives thereof, sobrerol, 3-cyclopropylmethylxanthine, hydrocortisone or salts thereof, piroxicam or a complex with cyclodextrins thereof, ketoprofen or a salt thereof, diclofenac or a salt thereof.

8. A composition according to claim 6 ox 7 wherein the active ingredient is contained in an amount ranging from 0.05% to 20% w/v.

9. A composition according to claim 6 or 7 wherein the active ingredient is contained in an amount ranging from 1% to 3% w/v.

10. The composition according to any one of claims 1 to 9, further comprising one or more adjuvants or excipients.