BR112013028544A2 - método para produzir um composto derivado de acetil-coa heterólogo - Google Patents
método para produzir um composto derivado de acetil-coa heterólogoInfo
- Publication number
- BR112013028544A2 BR112013028544A2 BR112013028544A BR112013028544A BR112013028544A2 BR 112013028544 A2 BR112013028544 A2 BR 112013028544A2 BR 112013028544 A BR112013028544 A BR 112013028544A BR 112013028544 A BR112013028544 A BR 112013028544A BR 112013028544 A2 BR112013028544 A2 BR 112013028544A2
- Authority
- BR
- Brazil
- Prior art keywords
- producing
- compounds
- pantothenate
- heterologous acetyl
- derivative compound
- Prior art date
Links
- 238000004519 manufacturing process Methods 0.000 title abstract 3
- -1 acetyl-coa derivative compound Chemical class 0.000 title 1
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical class OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 abstract 3
- 150000001875 compounds Chemical class 0.000 abstract 3
- ZSLZBFCDCINBPY-ZSJPKINUSA-N acetyl-CoA Chemical compound O[C@@H]1[C@H](OP(O)(O)=O)[C@@H](COP(O)(=O)OP(O)(=O)OCC(C)(C)[C@@H](O)C(=O)NCCC(=O)NCCSC(=O)C)O[C@H]1N1C2=NC=NC(N)=C2N=C1 ZSLZBFCDCINBPY-ZSJPKINUSA-N 0.000 abstract 2
- 239000006143 cell culture medium Substances 0.000 abstract 2
- 244000005700 microbiome Species 0.000 abstract 2
- 101100016385 Xenopus laevis hacd4 gene Proteins 0.000 abstract 1
- 230000002068 genetic effect Effects 0.000 abstract 1
- 238000000034 method Methods 0.000 abstract 1
- 230000000813 microbial effect Effects 0.000 abstract 1
- 229940014662 pantothenate Drugs 0.000 abstract 1
- 235000019161 pantothenic acid Nutrition 0.000 abstract 1
- 239000011713 pantothenic acid Substances 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/44—Preparation of O-glycosides, e.g. glucosides
- C12P19/60—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin
- C12P19/62—Preparation of O-glycosides, e.g. glucosides having an oxygen of the saccharide radical directly bound to a non-saccharide heterocyclic ring or a condensed ring system containing a non-saccharide heterocyclic ring, e.g. coumermycin, novobiocin the hetero ring having eight or more ring members and only oxygen as ring hetero atoms, e.g. erythromycin, spiramycin, nystatin
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/38—Chemical stimulation of growth or activity by addition of chemical compounds which are not essential growth factors; Stimulation of growth by removal of a chemical compound
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/52—Genes encoding for enzymes or proenzymes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P13/00—Preparation of nitrogen-containing organic compounds
- C12P13/04—Alpha- or beta- amino acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
- C12P17/02—Oxygen as only ring hetero atoms
- C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P5/00—Preparation of hydrocarbons or halogenated hydrocarbons
- C12P5/007—Preparation of hydrocarbons or halogenated hydrocarbons containing one or more isoprene units, i.e. terpenes
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P7/00—Preparation of oxygen-containing organic compounds
- C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
- C12P7/6409—Fatty acids
- C12P7/6427—Polyunsaturated fatty acids [PUFA], i.e. having two or more double bonds in their backbone
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Engineering & Computer Science (AREA)
- Life Sciences & Earth Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Biomedical Technology (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
método para produzir um composto derivado de acetil-coa heterólogo a presente divulgação relata o uso de compostos de pantotenato como um interruptor não genético para a produção de compostos derivados de acetil-coa heterólogo em células hospedeiras microbianas. a invenção provê microrganismos geneticamente modificados que são mais estáveis quando armazenados e cultivados inicialmente sob concentrações reduzidas de pantotenato, meio de cultura de célula tendo concentrações reduzidas de compostos de pantotenato e métodos para produzir compostos hacd usando o meio de cultura de célula e os microrganismos geneticamente modificados da invenção.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201161483896P | 2011-05-09 | 2011-05-09 | |
| US61/483896 | 2011-05-09 | ||
| PCT/US2012/037127 WO2012154854A1 (en) | 2011-05-09 | 2012-05-09 | Production of acetyl-coenzyme a derived compounds |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR112013028544A2 true BR112013028544A2 (pt) | 2016-09-20 |
| BR112013028544B1 BR112013028544B1 (pt) | 2020-10-27 |
Family
ID=47139628
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112013028544-3A BR112013028544B1 (pt) | 2011-05-09 | 2012-05-09 | método para produzir um composto derivado de acetilcoa heterólogo |
Country Status (11)
| Country | Link |
|---|---|
| US (1) | US9670518B2 (pt) |
| EP (1) | EP2707475B1 (pt) |
| JP (1) | JP5989098B2 (pt) |
| CN (1) | CN103502428A (pt) |
| AU (1) | AU2012253564A1 (pt) |
| BR (1) | BR112013028544B1 (pt) |
| CA (1) | CA2832979C (pt) |
| DK (1) | DK2707475T3 (pt) |
| MX (1) | MX2013012871A (pt) |
| WO (1) | WO2012154854A1 (pt) |
| ZA (1) | ZA201308393B (pt) |
Families Citing this family (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2834783A1 (en) * | 2011-05-13 | 2012-11-22 | Amyris, Inc. | Methods and compositions for detecting microbial production of water-immiscible compounds |
| WO2013181647A2 (en) | 2012-06-01 | 2013-12-05 | Danisco Us Inc. | Compositions and methods of producing isoprene and/or industrrial bio-products using anaerobic microorganisms |
| WO2014087184A1 (en) * | 2012-12-03 | 2014-06-12 | Metabolic Explorer | Neopentyl glycol fermentative production by a recombinant microorganism |
| CN104120148A (zh) * | 2013-04-28 | 2014-10-29 | 中国科学院青岛生物能源与过程研究所 | 一种生物法合成α-或β-蒎烯的方法 |
| EP4421181A2 (en) | 2014-09-18 | 2024-08-28 | Genomatica, Inc. | Non-natural microbial organisms with improved energetic efficiency |
| KR101782891B1 (ko) * | 2015-07-08 | 2017-09-29 | 한국과학기술연구원 | 미어신 생산용 벡터 및 균주, 및 이를 이용한 미어신의 생산방법 |
| CN107083338A (zh) * | 2017-05-15 | 2017-08-22 | 天津大学 | 重组菌株及其构建方法与在产菜油甾醇中的应用 |
| EP3720944A1 (en) | 2017-12-07 | 2020-10-14 | Zymergen Inc. | Engineered biosynthetic pathways for production of (6e)-8-hydroxygeraniol by fermentation |
| EP3728212A1 (en) | 2017-12-21 | 2020-10-28 | Zymergen Inc. | Nepetalactol oxidoreductases, nepetalactol synthases, and microbes capable of producing nepetalactone |
| CN110656056B (zh) * | 2019-10-31 | 2021-07-27 | 江南大学 | 一种高浓度蒎烯耐受性产蒎烯工程菌的构建方法 |
| CN112402401B (zh) * | 2020-12-18 | 2022-03-22 | 中国农业科学院兰州畜牧与兽药研究所 | 桧烯在制备杀灭或预防寄生虫病药物中的应用 |
| CN116926092B (zh) * | 2022-10-28 | 2024-04-26 | 昆明理工大学 | 一种泛酸激酶基因RkPank及其应用 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| AU4863099A (en) * | 1998-07-06 | 2000-01-24 | Dcv, Inc. Doing Business As Bio-Technical Resources | Method of vitamin production |
| EP2305825B1 (en) * | 1998-07-06 | 2015-01-14 | DCV Inc. doing business as Bio-Technical Resourses | Method of vitamin production |
| PL205622B1 (pl) * | 2000-07-25 | 2010-05-31 | Emmaus Foundation | Ciągły sposób wytwarzania etanolu w beztlenowej fermentacji bakteryjnej |
| US20040199941A1 (en) * | 2003-03-24 | 2004-10-07 | Rice University | Increased bacterial CoA and acetyl-CoA pools |
| US8143035B2 (en) * | 2006-09-22 | 2012-03-27 | Rice University | Pantothenate kinase overexpression and pantothenic acid supplementation in actinomycetes |
| WO2008039499A2 (en) * | 2006-09-26 | 2008-04-03 | The Regents Of The University Of California | Production of isoprenoids and isoprenoid precursors |
| AU2008305655B2 (en) * | 2007-09-20 | 2013-10-31 | Amyris, Inc. | Production of isoprenoids |
| US8481286B2 (en) * | 2008-08-12 | 2013-07-09 | Allylix, Inc. | Method for production of isoprenoid compounds |
-
2012
- 2012-05-09 WO PCT/US2012/037127 patent/WO2012154854A1/en active Application Filing
- 2012-05-09 JP JP2014510442A patent/JP5989098B2/ja active Active
- 2012-05-09 CA CA2832979A patent/CA2832979C/en active Active
- 2012-05-09 BR BR112013028544-3A patent/BR112013028544B1/pt active IP Right Grant
- 2012-05-09 DK DK12782714.5T patent/DK2707475T3/en active
- 2012-05-09 AU AU2012253564A patent/AU2012253564A1/en not_active Abandoned
- 2012-05-09 MX MX2013012871A patent/MX2013012871A/es active IP Right Grant
- 2012-05-09 EP EP12782714.5A patent/EP2707475B1/en active Active
- 2012-05-09 CN CN201280022308.5A patent/CN103502428A/zh active Pending
- 2012-05-09 US US13/467,783 patent/US9670518B2/en active Active
-
2013
- 2013-11-07 ZA ZA2013/08393A patent/ZA201308393B/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| JP2014513542A (ja) | 2014-06-05 |
| EP2707475A4 (en) | 2014-12-03 |
| DK2707475T3 (en) | 2016-01-11 |
| CA2832979C (en) | 2020-04-14 |
| CN103502428A (zh) | 2014-01-08 |
| EP2707475B1 (en) | 2015-09-30 |
| EP2707475A1 (en) | 2014-03-19 |
| WO2012154854A1 (en) | 2012-11-15 |
| ZA201308393B (en) | 2014-08-27 |
| BR112013028544B1 (pt) | 2020-10-27 |
| AU2012253564A1 (en) | 2013-10-31 |
| US20120288891A1 (en) | 2012-11-15 |
| JP5989098B2 (ja) | 2016-09-07 |
| US9670518B2 (en) | 2017-06-06 |
| MX2013012871A (es) | 2014-02-17 |
| CA2832979A1 (en) | 2012-11-15 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B06T | Formal requirements before examination [chapter 6.20 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 09/05/2012, OBSERVADAS AS CONDICOES LEGAIS. |