BR112012025820A2 - método de analisar uma célula ou outro material biológico compreendendo um ácido nucleico - Google Patents
método de analisar uma célula ou outro material biológico compreendendo um ácido nucleicoInfo
- Publication number
- BR112012025820A2 BR112012025820A2 BR112012025820A BR112012025820A BR112012025820A2 BR 112012025820 A2 BR112012025820 A2 BR 112012025820A2 BR 112012025820 A BR112012025820 A BR 112012025820A BR 112012025820 A BR112012025820 A BR 112012025820A BR 112012025820 A2 BR112012025820 A2 BR 112012025820A2
- Authority
- BR
- Brazil
- Prior art keywords
- analyzing
- cell
- nucleic acid
- biological material
- atom
- Prior art date
Links
- 239000012620 biological material Substances 0.000 title abstract 2
- 150000007523 nucleic acids Chemical class 0.000 title abstract 2
- 102000039446 nucleic acids Human genes 0.000 title abstract 2
- 108020004707 nucleic acids Proteins 0.000 title abstract 2
- 125000004435 hydrogen atom Chemical class [H]* 0.000 abstract 3
- 125000002887 hydroxy group Chemical group [H]O* 0.000 abstract 3
- 125000002947 alkylene group Chemical group 0.000 abstract 2
- 229910052799 carbon Inorganic materials 0.000 abstract 2
- 229910052739 hydrogen Inorganic materials 0.000 abstract 2
- 239000001257 hydrogen Substances 0.000 abstract 2
- 125000000229 (C1-C4)alkoxy group Chemical group 0.000 abstract 1
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 abstract 1
- 125000004423 acyloxy group Chemical group 0.000 abstract 1
- 125000000217 alkyl group Chemical group 0.000 abstract 1
- -1 amino halogen Chemical class 0.000 abstract 1
- 150000001450 anions Chemical class 0.000 abstract 1
- 125000004429 atom Chemical group 0.000 abstract 1
- 150000001721 carbon Chemical group 0.000 abstract 1
- 150000001875 compounds Chemical class 0.000 abstract 1
- 125000004990 dihydroxyalkyl group Chemical group 0.000 abstract 1
- 229910052736 halogen Inorganic materials 0.000 abstract 1
- 125000000623 heterocyclic group Chemical group 0.000 abstract 1
- 229910052757 nitrogen Inorganic materials 0.000 abstract 1
- 125000004433 nitrogen atom Chemical group N* 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B1/00—Dyes with anthracene nucleus not condensed with any other ring
- C09B1/16—Amino-anthraquinones
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B1/00—Dyes with anthracene nucleus not condensed with any other ring
- C09B1/16—Amino-anthraquinones
- C09B1/20—Preparation from starting materials already containing the anthracene nucleus
- C09B1/207—Dyes with amino groups and with onium groups
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K11/00—Luminescent, e.g. electroluminescent, chemiluminescent materials
- C09K11/06—Luminescent, e.g. electroluminescent, chemiluminescent materials containing organic luminescent materials
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09K—MATERIALS FOR MISCELLANEOUS APPLICATIONS, NOT PROVIDED FOR ELSEWHERE
- C09K2211/00—Chemical nature of organic luminescent or tenebrescent compounds
- C09K2211/10—Non-macromolecular compounds
- C09K2211/1003—Carbocyclic compounds
- C09K2211/1011—Condensed systems
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N2001/302—Stain compositions
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Urology & Nephrology (AREA)
- Hematology (AREA)
- Materials Engineering (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- Medicinal Chemistry (AREA)
- Food Science & Technology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
método de analisar uma célula ou outro material biológical compreendendo um ácido nucleico. de acordo com a invenção, é fornecido um composto de f´romula (i) em que a é um grupo alquileno c~ 2~-~ 8~; r^ 1^, r^ 2^, r^ 3^ e r^ 4^ são independentemente selecionados de hidrogênio, alquila c~ 1~-~ 4~ e di-hidroxialquila c~ 2~-~ 4~, em que o átomo de carbono ligado ao átomo de hidrogênio não é substituído com um grupo hidroxila e nenhum átomo de carbono é subsbtituído com dois grupos hidroxila, ou r^ 2^ e r^ 3^ juntos formam um grupo alquileno c~ 2~-~ 6~, que com o átomo de nitrogênio ao qual r^ 2^ e r^ 3^estão ligados formam um anel heterocíclico; x~ 1~, x~ 2~ e x~ 3~ são independentemente selecionados de hidrogênio, hidroxila, nr^ 1^-a- nr^ 2^r^ 3^r^ 4^ + (z^ m-^) 1/m, amino halogênio, alcóxi c~ 1~-~ 4~ ou alcanoiloxi c~ 2~-~ 8~; e (z^ m-^) 1/m é um ânion de carga m; ou um derivado cujo grupo nr^ 1^ é queternizado.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| GB1005939.2 | 2010-04-09 | ||
| GBGB1005939.2A GB201005939D0 (en) | 2010-04-09 | 2010-04-09 | Method of analysing a cell or other biological material containing nucleic acid |
| PCT/GB2011/050702 WO2011124927A1 (en) | 2010-04-09 | 2011-04-08 | Method of analysing a cell or other biological material containing a nucleic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR112012025820A2 true BR112012025820A2 (pt) | 2016-06-28 |
| BR112012025820B1 BR112012025820B1 (pt) | 2020-05-12 |
Family
ID=42236073
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BR112012025820-6A BR112012025820B1 (pt) | 2010-04-09 | 2011-04-08 | Composto, composição e complexo fluorescente compreendendo o mesmo, método de fabricação do composto e método de analisar uma célula ou outro material biológico compreendendo um ácido nucleico |
Country Status (16)
| Country | Link |
|---|---|
| US (2) | US9051467B2 (pt) |
| EP (2) | EP2556047B1 (pt) |
| JP (2) | JP6033767B2 (pt) |
| KR (2) | KR101957288B1 (pt) |
| CN (1) | CN102947266B (pt) |
| AU (2) | AU2011236610B2 (pt) |
| BR (1) | BR112012025820B1 (pt) |
| CA (1) | CA2797440C (pt) |
| DK (2) | DK2556047T3 (pt) |
| ES (1) | ES2676351T3 (pt) |
| GB (1) | GB201005939D0 (pt) |
| HU (1) | HUE038706T2 (pt) |
| NO (1) | NO2556047T3 (pt) |
| PL (1) | PL2556047T3 (pt) |
| PT (1) | PT2556047T (pt) |
| WO (1) | WO2011124927A1 (pt) |
Families Citing this family (14)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201005939D0 (en) * | 2010-04-09 | 2010-05-26 | Biostatus Ltd | Method of analysing a cell or other biological material containing nucleic acid |
| US8790892B2 (en) | 2011-04-04 | 2014-07-29 | Biotium, Inc. | Substituted anthraquinone dyes for cellular stains and enzyme detection |
| JP2013094135A (ja) * | 2011-11-02 | 2013-05-20 | Waseda Univ | 人工ナノ材料の安全性を評価する方法 |
| US9933341B2 (en) | 2012-04-05 | 2018-04-03 | Becton, Dickinson And Company | Sample preparation for flow cytometry |
| AU2013243378B2 (en) * | 2012-04-05 | 2019-04-11 | Becton, Dickinson And Company | Sample preparation for flow cytometry |
| US8993259B2 (en) | 2012-05-02 | 2015-03-31 | Charles River Laboratories, Inc. | Method of viability staining with membrane permeable fluorescent dye and membrane impermeable fluorescence quencher |
| EP2780707B1 (en) | 2012-05-02 | 2016-02-24 | Charles River Laboratories, Inc. | Method of detecting viable cells in a cell sample |
| JP6240162B2 (ja) | 2012-05-02 | 2017-11-29 | チャールズ リバー ラボラトリーズ， インコーポレイテッド | 細胞収集システムおよびその使用 |
| GB201214169D0 (en) | 2012-08-08 | 2012-09-19 | Biostatus Ltd | New compounds and uses thereof |
| WO2015095152A1 (en) * | 2013-12-17 | 2015-06-25 | The Penn State Research Foundation | Manipulation of light spectral quality to reduce parasitism by cuscuta and other plant parasites |
| GB201610971D0 (en) | 2016-06-23 | 2016-08-10 | Biostatus Ltd | Compounds |
| CN111117284B (zh) * | 2019-11-21 | 2021-08-06 | 苏州优逸兰迪生物科技有限公司 | RedNucleusⅡ染料及其制备方法与基于该染料的细胞凋亡检测试剂盒 |
| CN112679543B (zh) * | 2020-12-29 | 2022-07-22 | 天津全和诚科技有限责任公司 | 一种亲脂性荧光素探针及其制备方法 |
| CN113072937B (zh) * | 2021-04-12 | 2022-06-07 | 郑州大学 | 一种脂滴靶向碳点、制备方法及应用 |
Family Cites Families (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3123605A (en) * | 1960-01-08 | 1964-03-03 | Basic anthraquinone dyestuffs | |
| GB1053300A (pt) * | 1962-10-29 | |||
| NL126022C (pt) * | 1962-10-29 | |||
| FR1379649A (fr) * | 1963-06-20 | 1964-11-27 | Oreal | Nouveaux colorants anthraquinoniques et leur utilisation pour la teinture des cheveux |
| JPS503337B1 (pt) * | 1964-08-06 | 1975-02-03 | ||
| US3646072A (en) * | 1970-07-27 | 1972-02-29 | Du Pont | Turquoise biscationic anthraquinone dyes |
| JPS503337A (pt) | 1973-05-11 | 1975-01-14 | ||
| US4123222A (en) | 1976-03-23 | 1978-10-31 | Ciba-Geigy Corporation | Process for the dyeing or printing of polyacrylonitrile material |
| FR2431128A1 (fr) * | 1978-07-13 | 1980-02-08 | Oreal | Procede d'evaluation de l'etat ionique des cheveux |
| US4353708A (en) * | 1978-07-13 | 1982-10-12 | L'oreal | Evaluation of the state of change of keratin fibers |
| US5057413A (en) | 1988-06-13 | 1991-10-15 | Becton, Dickinson And Company | Method for discriminating between intact and damaged cells in a sample |
| GB8923075D0 (en) | 1989-10-13 | 1989-11-29 | Patterson Laurence H | Anti-cancer compounds |
| JPH04136802A (ja) * | 1990-09-28 | 1992-05-11 | Nippon Kayaku Co Ltd | 着色フィルター |
| US5520707A (en) * | 1995-08-07 | 1996-05-28 | Clairol, Inc. | Methods for dyeing hair with anthraquinone hair dyes having a quaternary ammonium side chain |
| GB9813062D0 (en) * | 1998-06-18 | 1998-08-19 | Univ Wales Medicine | An anthraquinone and its derivatives |
| MXPA01005691A (es) * | 1998-12-07 | 2002-04-24 | Eastman Chem Co | Una composicion colorante, un monocompuesto polimero ique consta de una composicion colorante y articulos producidos de el. |
| US6562785B1 (en) * | 1999-03-23 | 2003-05-13 | Howard M. Shapiro | Method for overcoming bacterial antibiotic resistance |
| AU2001235997B2 (en) * | 2000-02-28 | 2005-06-09 | Daiichi Pure Chemicals Co., Ltd. | Fluorescent probes for the quantitation of zinc |
| US6949250B2 (en) * | 2002-12-16 | 2005-09-27 | The United States Of America As Represented By The Secretary Of Agriculture | Selective algaecides for control of cyanochloronta |
| US20070082377A1 (en) | 2005-10-07 | 2007-04-12 | Olin Michael R | Cytotoxicity assays |
| US9334281B2 (en) * | 2008-09-08 | 2016-05-10 | Enzo Life Sciences, Inc. | Fluorochromes for organelle tracing and multi-color imaging |
| GB201005939D0 (en) * | 2010-04-09 | 2010-05-26 | Biostatus Ltd | Method of analysing a cell or other biological material containing nucleic acid |
-
2010
- 2010-04-09 GB GBGB1005939.2A patent/GB201005939D0/en not_active Ceased
-
2011
- 2011-04-08 WO PCT/GB2011/050702 patent/WO2011124927A1/en active Application Filing
- 2011-04-08 DK DK11720571.6T patent/DK2556047T3/en active
- 2011-04-08 CA CA2797440A patent/CA2797440C/en active Active
- 2011-04-08 KR KR1020187009266A patent/KR101957288B1/ko active IP Right Grant
- 2011-04-08 PL PL11720571T patent/PL2556047T3/pl unknown
- 2011-04-08 BR BR112012025820-6A patent/BR112012025820B1/pt active IP Right Grant
- 2011-04-08 CN CN201180028424.3A patent/CN102947266B/zh active Active
- 2011-04-08 DK DK18167949.9T patent/DK3366667T3/da active
- 2011-04-08 HU HUE11720571A patent/HUE038706T2/hu unknown
- 2011-04-08 AU AU2011236610A patent/AU2011236610B2/en active Active
- 2011-04-08 JP JP2013503178A patent/JP6033767B2/ja active Active
- 2011-04-08 EP EP11720571.6A patent/EP2556047B1/en active Active
- 2011-04-08 PT PT117205716T patent/PT2556047T/pt unknown
- 2011-04-08 KR KR1020127029033A patent/KR101846491B1/ko active IP Right Grant
- 2011-04-08 ES ES11720571.6T patent/ES2676351T3/es active Active
- 2011-04-08 US US13/639,872 patent/US9051467B2/en active Active
- 2011-04-08 EP EP18167949.9A patent/EP3366667B1/en active Active
- 2011-04-08 NO NO11720571A patent/NO2556047T3/no unknown
-
2015
- 2015-06-05 US US14/731,647 patent/US11255761B2/en active Active
-
2016
- 2016-10-26 JP JP2016209377A patent/JP6586070B2/ja active Active
- 2016-10-26 AU AU2016250384A patent/AU2016250384B2/en active Active
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B06G | Technical and formal requirements: other requirements [chapter 6.7 patent gazette] |
Free format text: SOLICITA-SE PAGAMENTE DE TAXA DE EXAME REFERENTE A QUANTIDADE ADICIONAL DE REIVINDICACOES SUBMETIDA A EXAME. |
|
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 08/04/2011, OBSERVADAS AS CONDICOES LEGAIS. |