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BR112014003911A2 - método integrado para a identificação de alto rendimento de novas composições pesticidas e seu uso - Google Patents

método integrado para a identificação de alto rendimento de novas composições pesticidas e seu uso

Info

Publication number
BR112014003911A2
BR112014003911A2 BR112014003911A BR112014003911A BR112014003911A2 BR 112014003911 A2 BR112014003911 A2 BR 112014003911A2 BR 112014003911 A BR112014003911 A BR 112014003911A BR 112014003911 A BR112014003911 A BR 112014003911A BR 112014003911 A2 BR112014003911 A2 BR 112014003911A2
Authority
BR
Brazil
Prior art keywords
methods
compositions
biotoxins
novel
identification
Prior art date
Application number
BR112014003911A
Other languages
English (en)
Inventor
Schwartz Ariel
J Grandlic Christopher
S Kerovuo Janne
Richardson Toby
Original Assignee
Synthetic Genomics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Synthetic Genomics Inc filed Critical Synthetic Genomics Inc
Publication of BR112014003911A2 publication Critical patent/BR112014003911A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Pest Control & Pesticides (AREA)
  • Insects & Arthropods (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)

Abstract

integrated method for high-throughput identification of novel pesticidal compositions and uses therefor abstract methods to rapidly identify nucleic acid sequences encoding novel biotoxins are provided. particularly, methods to rapidly sample and screen extrachromosomal genetic content of microorganisms for novel sequences of interest are described. compositions comprising coding sequences for biotoxins, and polypeptides and uses derived therefrom are provided. compositions and methods are useful, for example, for conferring pesticidal activity to bacteria, plants, plant cells, tissues, and seeds. tradução do resumo resumo patente de invenção: "método integrado para a identificação de alto rendimento de novas composições pesticidas e seu uso". são fornecidos métodos para a identificação rápida das seqüências de ácido nucléico que codificam as novas biotoxinas. particularmente, métodos para rapidamente tirar uma amostra e peneirar o conteúdo genético extracromossômico de microorganismos para novas seqüências de interesse são descritos. as composições compreendendo as seqüências de codificação para biotoxinas e polipeptídeos e uso derivado destas são fornecidas. as composições e os métodos são úteis, por exemplo, para conferir uma atividade pesticida às bactérias, plantas, células vegetais, tecidos e sementes.
BR112014003911A 2011-08-19 2012-08-17 método integrado para a identificação de alto rendimento de novas composições pesticidas e seu uso BR112014003911A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201161525674P 2011-08-19 2011-08-19
PCT/US2012/051466 WO2013028563A2 (en) 2011-08-19 2012-08-17 Integrated method for high-throughput identification of novel pesticidal compositions and uses therefor

Publications (1)

Publication Number Publication Date
BR112014003911A2 true BR112014003911A2 (pt) 2017-03-14

Family

ID=47747053

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112014003911A BR112014003911A2 (pt) 2011-08-19 2012-08-17 método integrado para a identificação de alto rendimento de novas composições pesticidas e seu uso

Country Status (10)

Country Link
US (1) US20130227743A1 (pt)
EP (1) EP2744920A4 (pt)
JP (1) JP6230125B2 (pt)
CN (1) CN103946393A (pt)
AU (2) AU2012299142A1 (pt)
BR (1) BR112014003911A2 (pt)
CA (1) CA2844913A1 (pt)
EA (1) EA025208B1 (pt)
MX (1) MX2014002027A (pt)
WO (1) WO2013028563A2 (pt)

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WO2016154602A1 (en) * 2015-03-26 2016-09-29 Epibiome, Inc. Compositions and methods for pest control
MA44954A (fr) * 2015-04-17 2019-03-20 Agbiome Inc Gènes pesticides et leurs procédés d'utilisation
US20160345590A1 (en) * 2015-05-01 2016-12-01 Board Of Regents, The University Of Texas System Chlamydomonas Strains With Chloroplast-Expressed Cry Proteins For Biological Control Of Mosquitoes That Transmit Disease
EP3303595A2 (en) * 2015-06-03 2018-04-11 Agbiome, Inc. Pesticidal genes and methods of use
MA49662A (fr) * 2015-06-22 2020-06-03 Agbiome Inc Gènes pesticides et leurs procédés d'utilisation
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AU2016338418B2 (en) * 2015-10-14 2021-12-16 BASF Agricultural Solutions Seed US LLC Axmi554 delta-endotoxin gene and methods for its use
US20190136259A1 (en) * 2016-05-17 2019-05-09 Pioneer Hi-Bred International, Inc. Methods of using cyt1a mutants against coleopteran pests
CA3035896A1 (en) * 2016-09-06 2018-03-15 AgBiome, Inc. Pesticidal genes and methods of use
WO2019126479A1 (en) * 2017-12-22 2019-06-27 AgBiome, Inc. Pesticidal genes and methods of use
CN108359673A (zh) * 2018-05-08 2018-08-03 江苏省农业科学院 一种高效杀食用菌眼蕈蚊的Bt cry11基因、编码蛋白及其应用
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WO2022072480A1 (en) * 2020-09-30 2022-04-07 Zymergen Inc. Methods to identify novel insecticidal proteins from complex metagenomic microbial samples
WO2024035660A2 (en) * 2022-08-06 2024-02-15 The Johns Hopkins University Methods, systems and kits for identifying bio active compounds and therapeutic methods and compositions

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Also Published As

Publication number Publication date
JP2014526893A (ja) 2014-10-09
AU2012299142A1 (en) 2014-02-27
EA201490480A1 (ru) 2014-06-30
MX2014002027A (es) 2014-11-10
WO2013028563A2 (en) 2013-02-28
WO2013028563A3 (en) 2014-05-15
AU2018200012A1 (en) 2018-01-25
JP6230125B2 (ja) 2017-11-15
CN103946393A (zh) 2014-07-23
EP2744920A2 (en) 2014-06-25
EA025208B1 (ru) 2016-11-30
CA2844913A1 (en) 2013-02-28
EP2744920A4 (en) 2015-06-03
AU2018200012A8 (en) 2018-02-22
US20130227743A1 (en) 2013-08-29

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06T Formal requirements before examination [chapter 6.20 patent gazette]
B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B11T Dismissal of application maintained [chapter 11.20 patent gazette]