AU783709B2 - Corynebacterium glutamicum genes encoding metabolic pathway proteins - Google Patents

Description

WO 01/00843 PCT/IB00/00923 r CORYNEBACTERIUM GLUTAMICUM GENES ENCODING METABOLIC PATHWAY PROTEINS Related Applications The present application claims priority to prior filed U.S. Provisional Patent Application Serial No. 60/141031, filed June 25, 1999, U.S. Provisional Patent Application Serial No. 60/142101, filed July 2, 1999, U.S. Provisional Patent Application Serial No. 60/148613, filed August 12, 1999, and also to U.S. Provisional Patent Application Serial No. 60/187970, filed March 9, 2000. The present application also claims priority to prior filed German Patent Application No. 19930476.9, filed July 1, 1999, German Patent Application No. 19931415.2, filed July 8, 1999, German Patent Application No. 19931418.7, filed July 8, 1999, German Patent Application No.

19931419.5, filed July 8, 1999, German Patent Application No. 19931420.9, filed July 8, 1999, German Patent Application No. 19931424.1, filed July 8, 1999, German Patent Application No. 19931428.4, filed July 8, 1999, German Patent Application No.

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19932130.2, filed July 9, 1999, German Patent Application No. 19932186.8, filed July 9, 1999, German Patent Application No. 19932206.6, filed July 9, 1999, German Patent Application No. 19932227.9, filed July 9, 1999, German Patent Application No.

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19933006.9, filed July 14, 1999, German Patent Application No. 19940764.9, filed August 27, 1999, German Patent Application No. 19940765.7, filed August 27, 1999, German Patent Application No. 19940766.5, filed August 27, 1999, German Patent Application No. 19940832.7, filed August 27, 1999, German Patent Application No.

19941378.9, filed August 31, 1999, German Patent Application No. 19941379.7, filed August 31, 1999, German Patent Application No. 19941380.0, filed August 31, 1999, German Patent Application No. 19941394.0, filed August 31, 1999, German Patent Application No. 19941396.7, filed August 31, 1999, German Patent Application No.

19942076.9, filed September 3, 1999, German Patent Application No. 19942077.7, filed September 3, 1999, German Patent Application No. 19942079.3, filed September 3, 1999, German Patent Application No. 19942086.6, filed September 3, 1999, German Patent Application No. 19942087.4, filed September 3, 1999, German Patent Application No. 19942088.2, filed September 3, 1999, German Patent Application No.

19942095.5, filed September 3, 1999, German Patent Application No. 19942124.2, filed September 3, 1999, and German Patent Application No. 19942129.3, filed September 3, 1999. The entire contents of all of the aforementioned applications are hereby expressly incorporated herein by this reference.

Background of the Invention Certain products and by-products of naturally-occurring metabolic processes in cells have utility in a wide array of industries, including the food, feed, cosmetics, and pharmaceutical industries. These molecules, collectively termed 'fine chemicals', include organic acids, both proteinogenic and non-proteinogenic amino acids, nucleotides and nucleosides, lipids and fatty acids, diols, carbohydrates, aromatic compounds, vitamins and cofactors, and enzymes. Their production is most conveniently performed through large-scale culture of bacteria developed to produce and secrete large quantities of a particular desired molecule. One particularly useful organism for this purpose is Corynebacterium glutamicum, a gram positive, nonpathogenic bacterium. Through strain selection, a number of mutant strains have WO 01/00843 PCT/IB00/00923 -3been developed which produce an array of desirable compounds. However, selection of strains improved for the production of a particular molecule is a time-consuming and difficult process.

Summary of the Invention The invention provides novel bacterial nucleic acid molecules which have a variety of uses. These uses include the identification of microorganisms which can be used to produce fine chemicals, the modulation of fine chemical production in C.

glutamicum or related bacteria, the typing or identification of C. glutamicum or related bacteria, as reference points for mapping the C. glutamicum genome, and as markers for transformation. These novel nucleic acid molecules encode proteins, referred to herein as metabolic pathway (MP) proteins.

C. glutamicum is a gram positive, aerobic bacterium which is commonly used in industry for the large-scale production of a variety of fine chemicals, and also for the degradation of hydrocarbons (such as in petroleum spills) and for the oxidation of terpenoids. The MP nucleic acid molecules of the invention, therefore, can be used to identify microorganisms which can be used to produce fine chemicals, by fermentation processes. Modulation of the expression of the MP nucleic acids of the invention, or modification of the sequence of the MP nucleic acid molecules of the invention, can be used to modulate the production of one or more fine chemicals from a microorganism to improve the yield or production of one or more fine chemicals from a Corynebacterium or Brevibacterium species).

The MP nucleic acids of the invention may also be used to identify an organism as being Corynebacterium glutamicum or a close relative thereof, or to identify the presence of C glutamicum or a relative thereof in a mixed population of microorganisms. The invention provides the nucleic acid sequences of a number of C glutamicum genes; by probing the extracted genomic DNA of a culture of a unique or mixed population of microorganisms under stringent conditions with a probe spanning a region of a C glutamicum gene which is unique to this organism, one can ascertain whether this organism is present. Although Corynebacterium glutamicum itself is nonpathogenic, it is related to species pathogenic in humans, such as Corynebacterium WO 01/00843 PCT/IB00/00923 -4diphtheriae (the causative agent of diphtheria); the detection of such organisms is of significant clinical relevance.

The MP nucleic acid molecules of the invention may also serve as reference points for mapping of the C. glutamicum genome, or of genomes of related organisms.

Similarly, these molecules, or variants or portions thereof, may serve as markers for genetically engineered Corynebacterium or Brevibacterium species.

The MP proteins encoded by the novel nucleic acid molecules of the invention are capable of, for example, performing an enzymatic step involved in the metabolism of certain fine chemicals, including amino acids, vitamins, cofactors, nutraceuticals, nucleotides, nucleosides, and trehalose. Given the availability of cloning vectors for use in Corynebacterium glutamicum, such as those disclosed in Sinskey et al., U.S. Patent No. 4,649,119, and techniques for genetic manipulation of C glutamicum and the related Brevibacterium species lactofermentum) (Yoshihama et al, J. Bacteriol.

162: 591-597 (1985); Katsumata et al., J. Bacteriol. 159: 306-311 (1984); and Santamaria et al., J. Gen. Microbiol. 130: 2237-2246 (1984)), the nucleic acid molecules of the invention may be utilized in the genetic engineering of this organism to make it a better or more efficient producer of one or more fine chemicals.

This improved production or efficiency of production of a fine chemical may be due to a direct effect of manipulation of a gene of the invention, or it may be due to an indirect effect of such manipulation. Specifically, alterations in C. glutamicum metabolic pathways for amino acids, vitamins, cofactors, nucleotides, and trehalose may have a direct impact on the overall production of one or more of these desired compounds from this organism. For example, optimizing the activity of a lysine biosynthetic pathway protein or decreasing the activity of a lysine degradative pathway protein may result in an increase in the yield or efficiency of production of lysine from such an engineered organism. Alterations in the proteins involved in these metabolic pathways may also have an indirect impact on the production or efficiency of production of a desired fine chemical. For example, a reaction which is in competition for an intermediate necessary for the production of a desired molecule may be eliminated, or a pathway necessary for the production of a particular intermediate for a desired compound may be optimized. Further, modulations in the biosynthesis or degradation of, for example, an amino acid, a vitamin, or a nucleotide may increase the overall WO 01/00843 PCT/IB00/00923 ability of the microorganism to rapidly grow and divide, thus increasing the number and/or production capacities of the microorganism in culture and thereby increasing the possible yield of the desired fine chemical.

The nucleic acid and protein molecules of the invention may be utilized to directly improve the production or efficiency of production of one or more desired fine chemicals from Corynebacterium glutamicum. Using recombinant genetic techniques well known in the art, one or more of the biosynthetic or degradative enzymes of the invention for amino acids, vitamins, cofactors, nutraceuticals, nucleotides, nucleosides, or trehalose may be manipulated such that its function is modulated. For example, a biosynthetic enzyme may be improved in efficiency, or its allosteric control region destroyed such that feedback inhibition of production of the compound is prevented.

Similarly, a degradative enzyme may be deleted or modified by substitution, deletion, or addition such that its degradative activity is lessened for the desired compound without impairing the viability of the cell. In each case, the overall yield or rate of production of the desired fine chemical may be increased.

It is also possible that such alterations in the protein and nucleotide molecules of the invention may improve the production of other fine chemicals besides the amino acids, vitamins, cofactors, nutraceuticals, nucleotides, nucleosides, and trehalose through indirect mechanisms. Metabolism of any one compound is necessarily intertwined with other biosynthetic and degradative pathways within the cell, and necessary cofactors, intermediates, or substrates in one pathway are likely supplied or limited by another such pathway. Therefore, by modulating the activity of one or more of the proteins of the invention, the production or efficiency of activity of another fine chemical biosynthetic or degradative pathway may be impacted. For example, amino acids serve as the structural units of all proteins, yet may be present intracellularly in levels which are limiting for protein synthesis; therefore, by increasing the efficiency of production or the yields of one or more amino acids within the cell, proteins, such as biosynthetic or degradative proteins, may be more readily synthesized. Likewise, an alteration in a metabolic pathway enzyme such that a particular side reaction becomes more or less favored may result in the over- or under-production of one or more compounds which are utilized as intermediates or substrates for the production of a desired fine chemical.

12/10 '05 WED 11:20 FAX 81 2 9888 7600 WATERMARK L0004 6 This invention provides novel nucleic acid molecules which encode proteins, referred to herein as metabolic .pathway proteins which are capable of, for example, performing an enzymatic step involved in the metabolism of molecules important for the normal functioning of cells, such as amino acids, vitamins, cofactors, nucleotides and nucleosides, or trehalose. Nucleic acid molecules encoding an MP protein are referred to herein as MP nucleic acid molecules. In a preferred embodiment, the MP protein performs an enzymatic step related to the metabolism of one or more of the following: amino acids, vitamins, cofactors, nutraceuticals, nucleotides, nucleosides, and trehalose.

Examples of such proteins include those encoded by the genes set forth in Table 1.

The following embodiments of the invention, the subject of this application, are specifically disclosed herein: S. An isolated Corynebacterium glutamicum nucleic acid molecule S: 15 comprising the nucleotide sequence set forth in SEQ ID NO;1, or a complement thereof.

An isolated nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2, or a complement thereof.

20 An isolated nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2, or a complement thereof.

An isolated nucleic acid molecule comprising a nucleotide sequence which is functionally equivalent, and at least 50% identical, to the 25 entire nucleotide sequence set forth in SEQ ID NO:1, or a complement thereof.

An isolated nucleic acid molecule comprising a fragment of at least contiguous nucleotides of the nucleotide sequence set forth In SEQ ID NO:1, or a complement thereof.

An isolated polypeptide comprising a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2.

COMS ID No: SBMI-01718461 Received by IP Australia: Time 11:28 Date 2005-10-12 12/10 '05 WED 11:21 FAX 81 2 9888 7600 WATERMARK 11005 6a An isolated polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is functionally equivalent, and at least 50% identical, to the entire nucleotide sequence set forth in SEQ ID NO:1.

An isolated polypeptide comprising an amino acid sequence which is functionally equivalent, and at least 51% identical, to the entire amino acid sequence set forth in SEQ ID NO:2.

An isolated polypeptide comprising a fragment of at least contiguous amino acids of the amino acid sequence set forth in SEQ ID NO:2, wherein said polypeptide fragment maintains a biological activity of the polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2.

A host cell comprising a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:1, wherein the nucleic 15 acid molecule is disrupted.

S. A host cell comprising a nucleic acid. molecule comprising the nucleotide sequence set forth in SEQ ID NO:1, wherein the nucleic acid molecule comprises one or more nucleic acid modifications as compared to the nucleotide sequence set forth in SEQ ID NO:1.

A host cell comprising a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:1, wherein the regulatory region of the nucleic acid molecule is modified relative to the wild-type regulatory region of the molecule.

Accordingly, one aspect of the invention pertains to isolated nucleic acid 25 molecules cDNAs, DNAs, or RNAs) comprising a nucleotide sequence encoding an MP protein or biologically active portions thereof, as well as nucleic acid fragments suitable as primers or hybridization probes for the detection or amplification of MP encoding nucleic acid DNA or mRNA). In particularly preferred embodiments, the isolated nucleic acid molecule comprises one of the nucleotide sequences set forth as the odd-numbered SEQ ID NOs in the Sequence Listing SEQ ID NO:I, SEQ ID NO:3, SEQ ID NO:5, SEQ ID or the coding region or a complement thereof of one of these nucleotide sequences. In other particularly preferred embodiments, the isolated nucleic COMS ID No: SBMI-01718461 Received by IP Australia: Time 11:28 Date 2005-10-12 12/10 '05 WED 11:21 FAX 61 2 9888 7600 WATERMARK 1oo006 6b acidmolecule of the invention comprises a nucleotide sequence which hybridizes to or is at least about 50%, preferably at least about 60%, more preferably at least about 70%, 80% or 90%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to a nucleotide sequence set forth as an odd-numbered SEQ ID NO in the Sequence Listing SEQ ID NO:I, SEQ ID NO:3, SEQ ID NO:5, SEQ ID or a portion thereof. In other preferred embodiments, the isolated nucleic acid molecule encodes one of the amino acid sequences set forth as an even-numbered SEQ ID NO in the Sequence Listing SEQ ID NO:2, SEQ ID NO:4, SEQ ID NO:6, SEQ ID The preferred MP proteins of the present invention also preferably possess at least one of the MP activities described herein.

In another embodiment, the isolated nucleic acid molecule encodes a protein or portion thereof wherein the protein or portion thereof includes an amino acid sequence which is sufficiently homologous to an amino acid sequence of the *i invention a **o *g COMS ID No: SBMI-01718461 Received by IP Australia: Time 11:28 Date 2005-10-12 WO 01/00843 PCT/IB00/00923 -7sequence having an even-numbered SEQ ID NO: in the Sequence Listing), e.g., sufficiently homologous to an amino acid sequence of the invention such that the protein or portion thereof maintains an MP activity. Preferably, the protein or portion thereof encoded by the nucleic acid molecule maintains the ability to perform an enzymatic reaction in a amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathway. In one embodiment, the protein encoded by the nucleic acid molecule is at least about 50%, preferably at least about 60%, and more preferably at least about 70%, 80%, or 90% and most preferably at least about 95%, 96%, 97%, 98%, or 99% or more homologous to an amino acid sequence of the invention an entire amino acid sequence selected from those having an even-numbered SEQ ID NO in the Sequence Listing). In another preferred embodiment, the protein is a full length C. glutamicum protein which is substantially homologous to an entire amino acid sequence of the invention (encoded by an open reading frame shown in the corresponding odd-numbered SEQ ID NOs in the Sequence Listing SEQ ID NO: 1, SEQ ID NO:3, SEQ ID NO:5, SEQ ID In another preferred embodiment, the isolated nucleic acid molecule is derived from C glutamicum and encodes a protein an MP fusion protein) which includes a biologically active domain which is at least about 50% or more homologous to one of the amino acid sequences of the invention a sequence of one of the even-numbered SEQ ID NOs in the Sequence Listing) and is able to catalyze a reaction in a metabolic pathway for an amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose, or one or more of the activities set forth in Table 1, and which also includes heterologous nucleic acid sequences encoding a heterologous polypeptide or regulatory regions.

In another embodiment, the isolated nucleic acid molecule is at least nucleotides in length and hybridizes under stringent conditions to a nucleic acid molecule comprising a nucleotide sequence of the invention a sequence of an oddnumbered SEQ ID NO in the Sequence Listing). Preferably, the isolated nucleic acid molecule corresponds to a naturally-occurring nucleic acid molecule. More preferably, the isolated nucleic acid encodes a naturally-occurring C. glutamicum MP protein, or a biologically active portion thereof.

WO 01/00843 PCT/IB00/00923 -8- Another aspect of the invention pertains to vectors, recombinant expression vectors, containing the nucleic acid molecules of the invention, and host cells into which such vectors have been introduced. In one embodiment, such a host cell is used to produce an MP protein by culturing the host cell in a suitable medium. The MP protein can be then isolated from the medium or the host cell.

Yet another aspect of the invention pertains to a genetically altered microorganism in which an MP gene has been introduced or altered. In one embodiment, the genome of the microorganism has been altered by introduction of a nucleic acid molecule of the invention encoding wild-type or mutated MP sequence as a transgene. In another embodiment, an endogenous MP gene within the genome of the microorganism has been altered, functionally disrupted, by homologous recombination with an altered MP gene. In another embodiment, an endogenous or introduced MP gene in a microorganism has been altered by one or more point mutations, deletions, or inversions, but still encodes a functional MP protein. In still another embodiment, one or more of the regulatory regions a promoter, repressor, or inducer) of an MP gene in a microorganism has been altered by deletion, truncation, inversion, or point mutation) such that the expression of the MP gene is modulated. In a preferred embodiment, the microorganism belongs to the genus Corynebacterium or Brevibacterium, with Corynebacterium glutamicum being particularly preferred. In a preferred embodiment, the microorganism is also utilized for the production of a desired compound, such as an amino acid, with lysine being particularly preferred.

In another aspect, the invention provides a method of identifying the presence or activity of Cornyebacterium diphtheriae in a subject. This method includes detection of one or more of the nucleic acid or amino acid sequences of the invention the sequences set forth in the Sequence Listing as SEQ ID NOs 1 through 1156) in a subject, thereby detecting the presence or activity of Corynebacterium diphtheriae in the subject.

Still another aspect of the invention pertains to an isolated MP protein or a portion, a biologically active portion, thereof. In a preferred embodiment, the isolated MP protein or portion thereof can catalyze an enzymatic reaction involved in one or more pathways for the metabolism of an amino acid, a vitamin, a cofactor, a WO 01/00843 PCT/IBOO/00923 -9nutraceutical, a nucleotide, a nucleoside, or trehalose. In another preferred embodiment, the isolated MP protein or portion thereof is sufficiently homologous to an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: in the Sequence Listing) such that the protein or portion thereof maintains the ability to catalyze an enzymatic reaction involved in one or more pathways for the metabolism of an amino acid, a vitamin, a cofactor, a nutraceutical, a nucleotide, a nucleoside, or trehalose.

The invention also provides an isolated preparation of an MP protein. In preferred embodiments, the MP protein comprises an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing).

In another preferred embodiment, the invention pertains to an isolated full length protein which is substantially homologous to an entire amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) (encoded by an open reading frame set forth in a corresponding odd-numbered SEQ ID NO: of the Sequence Listing). In yet another embodiment, the protein is at least about preferably at least about 60%, and more preferably at least about 70%, 80%, or and most preferably at least about 95%, 96%, 97%, 98%, or 99% or more homologous to an entire amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing). In other embodiments, the isolated MP protein comprises an amino acid sequence which is at least about 50% or more homologous to one of the amino acid sequences of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) and is able to catalyze an enzymatic reaction in an amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathway, or has one or more of the activities set forth in Table 1.

Alternatively, the isolated MP protein can comprise an amino acid sequence which is encoded by a nucleotide sequence which hybridizes, hybridizes under stringent conditions, or is at least about 50%, preferably at least about 60%, more preferably at least about 70%, 80%, or 90%, and even more preferably at least about 96%, 97%, or 99% or more homologous to a nucleotide sequence of one of the even-numbered SEQ ID NOs set forth in the Sequence Listing. It is also preferred that the preferred forms of MP proteins also have one or more of the MP bioactivities described herein.

WO 01/00843 PCT/IB00/00923 The MP polypeptide, or a biologically active portion thereof, can be operatively linked to a non-MP polypeptide to form a fusion protein. In preferred embodiments, this fusion protein has an activity which differs from that of the MP protein alone. In other preferred embodiments, this fusion protein, when introduced into a C. glutamicum pathway for the metabolism of an amino acid, vitamin, cofactor, nutraceutical, results in increased yields and/or efficiency of production of a desired fine chemical from C.

glutamicum. In particularly preferred embodiments, integration of this fusion protein into an amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathway of a host cell modulates production of a desired compound from the cell.

In another aspect, the invention provides methods for screening molecules which modulate the activity of an MP protein, either by interacting with the protein itself or a substrate or binding partner of the MP protein, or by modulating the transcription or translation of an MP nucleic acid molecule of the invention.

Another aspect of the invention pertains to a method for producing a fine chemical. This method involves the culturing of a cell containing a vector directing the expression of an MP nucleic acid molecule of the invention, such that a fine chemical is produced. In a preferred embodiment, this method further includes the step of obtaining a cell containing such a vector, in which a cell is transfected with a vector directing the expression of an MP nucleic acid. In another preferred embodiment, this method further includes the step of recovering the fine chemical from the culture. In a particularly preferred embodiment, the cell is from the genus Corynebacterium or Brevibacterium, or is selected from those strains set forth in Table 3.

Another aspect of the invention pertains to methods for modulating production of a molecule from a microorganism. Such methods include contacting the cell with an agent which modulates MP protein activity or MP nucleic acid expression such that a cell associated activity is altered relative to this same activity in the absence of the agent. In a preferred embodiment, the cell is modulated for one or more C. glutamicum amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathways, such that the yields or rate of production of a desired fine chemical by this microorganism is improved. The agent which modulates MP protein activity can be an agent which stimulates MP protein activity or MP nucleic acid expression.

WO 01/00843 PCT/IB00/00923 -11- Examples of agents which stimulate MP protein activity or MP nucleic acid expression include small molecules, active MP proteins, and nucleic acids encoding MP proteins that have been introduced into the cell. Examples of agents which inhibit MP activity or expression include small molecules, and antisense MP nucleic acid molecules.

Another aspect of the invention pertains to methods for modulating yields of a desired compound from a cell, involving the introduction of a wild-type or mutant MP gene into a cell, either maintained on a separate plasmid or integrated into the genome of the host cell. If integrated into the genome, such integration can be random, or it can take place by homologous recombination such that the native gene is replaced by the introduced copy, causing the production of the desired compound from the cell to be modulated. In a preferred embodiment, said yields are increased. In another preferred embodiment, said chemical is a fine chemical. In a particularly preferred embodiment, said fine chemical is an amino acid. In especially preferred embodiments, said amino acid is L-lysine.

Detailed Description of the Invention The present invention provides MP nucleic acid and protein molecules which are involved in the metabolism of certain fine chemicals in Corynebacterium glutamicum, including amino acids, vitamins, cofactors, nutraceuticals, nucleotides, nucleosides, and trehalose. The molecules of the invention may be utilized in the modulation of production of fine chemicals from microorganisms, such as C. glutamicum, either directly where modulation of the activity of a lysine biosynthesis protein has a direct impact on the production or efficiency of production of lysine from that organism), or may have an indirect impact which nonetheless results in an increase of yield or efficiency of production of the desired compound where modulation of the activity of a nucleotide biosynihesis protein has an impact on the production of an organic acid or a fatty acid from the bacterium, perhaps due to improved growth or an increased supply of necessary co-factors, energy compounds, or precursor molecules).

Aspects of the invention are further explicated below.

WO 01/00843 PCT/IB00/00923 -12- I. Fine Chemicals The term 'fine chemical' is art-recognized and includes molecules produced by an organism which have applications in various industries, such as, but not limited to, the pharmaceutical, agriculture, and cosmetics industries. Such compounds include organic acids, such as tartaric acid, itaconic acid, and diaminopimelic acid, both proteinogenic and non-proteinogenic amino acids, purine and pyrimidine bases, nucleosides, and nucleotides (as described e.g. in Kuninaka, A. (1996) Nucleotides and related compounds, p. 561-612, in Biotechnology vol. 6, Rehm et al., eds. VCH: Weinheim, and references contained therein), lipids, both saturated and unsaturated fatty acids arachidonic acid), diols propane diol, and butane diol), carbohydrates hyaluronic acid and trehalose), aromatic compounds aromatic amines, vanillin, and indigo), vitamins and cofactors (as described in Ullmann's Encyclopedia of Industrial Chemistry, vol. A27, "Vitamins", p. 443-613 (1996) VCH: Weinheim and references therein; and Ong, Niki, E. Packer, L. (1995) "Nutrition, Lipids, Health, and Disease" Proceedings of the UNESCO/Confederation of Scientific and Technological Associations in Malaysia, and the Society for Free Radical Research Asia, held Sept. 1-3, 1994 at Penang, Malaysia, AOCS Press, (1995)), enzymes, polyketides (Cane et al. (1998) Science 282: 63-68), and all other chemicals described in Gutcho (1983) Chemicals by Fermentation, Noyes Data Corporation, ISBN: 0818805086 and references therein. The metabolism and uses of certain of these fine chemicals are further explicated below.

A. Amino Acid Metabolism and Uses Amino acids comprise the basic structural units of all proteins, and as such are essential for normal cellular functioning in all organisms. The term "amino acid" is artrecognized. The proteinogenic amino acids, of which there are 20 species, serve as structural units for proteins, in which they are linked by peptide bonds, while the nonproteinogenic amino acids (hundreds of which are known) are not normally found in proteins (see Ulmann's Encyclopedia of Industrial Chemistry, vol. A2, p. 57-97 VCH: Weinheim (1985)). Amino acids may be in the D- or L- optical configuration, though Lamino acids are generally the only type found in naturally-occurring proteins.

Biosynthetic and degradative pathways of each of the 20 proteinogenic amino acids WO 01/00843 PCT/IB00/00923 -13have been well characterized in both prokaryotic and eukaryotic cells (see, for example, Stryer, L. Biochemistry, 3 r edition, pages 578-590 (1988)). The 'essential' amino acids (histidine, isoleucine, leucine, lysine, methionine, phenylalanine, threonine, tryptophan, and valine), so named because they are generally a nutritional requirement due to the complexity of their biosyntheses, are readily converted by simple biosynthetic pathways to the remaining 11 'nonessential' amino acids (alanine, arginine, asparagine, aspartate, cysteine, glutamate, glutamine, glycine, proline, serine, and tyrosine). Higher animals do retain the ability to synthesize some of these amino acids, but the essential amino acids must be supplied from the diet in order for normal protein synthesis to occur.

Aside from their function in protein biosynthesis, these amino acids are interesting chemicals in their own right, and many have been found to have various applications in the food, feed, chemical, cosmetics, agriculture, and pharmaceutical industries. Lysine is an important amino acid in the nutrition not only of humans, but also of monogastric animals such as poultry and swine. Glutamate is most commonly used as a flavor additive (mono-sodium glutamate, MSG) and is widely used throughout the food industry, as are aspartate, phenylalanine, glycine, and cysteine. Glycine, Lmethionine and tryptophan are all utilized in the pharmaceutical industry. Glutamine, valine, leucine, isoleucine, histidine, arginine, proline, serine and alanine are of use in both the pharmaceutical and cosmetics industries. Threonine, tryptophan, and D/ Lmethionine are common feed additives. (Leuchtenberger, W. (1996) Amino aids technical production and use, p. 466-502 in Rehm et al. (eds.) Biotechnology vol. 6, chapter 14a, VCH: Weinheim). Additionally, these amino acids have been found to be useful as precursors for the synthesis of synthetic amino acids and proteins, such as Nacetylcysteine, S-carboxymethyl-L-cysteine, (S)-5-hydroxytryptophan, and others described in Ulmann's Encyclopedia of Industrial Chemistry, vol. A2, p. 57-97, VCH: Weinheim, 1985.

The biosynthesis of these natural amino acids in organisms capable of producing them, such as bacteria, has been well characterized (for review of bacterial amino acid biosynthesis and regulation thereof, see Umbarger, H.E.(1978) Ann. Rev.

Biochem. 47: 533-606). Glutamate is synthesized by the reductive amination of aketoglutarate, an intermediate in the citric acid cycle. Glutamine, proline, and arginine are each subsequently produced from glutamate. The biosynthesis of serine is a three- WO 01/00843 PCT/IB00/00923 14step process beginning with 3-phosphoglycerate (an intermediate in glycolysis), and resulting in this amino acid after oxidation, transamination, and hydrolysis steps. Both cysteine and glycine are produced from serine; the former by the condensation of homocysteine with serine, and the latter by the transferal of the side-chain P-carbon atom to tetrahydrofolate, in a reaction catalyzed by serine transhydroxymethylase.

Phenylalanine, and tyrosine are synthesized from the glycolytic and pentose phosphate pathway precursors erythrose 4-phosphate and phosphoenolpyruvate in a 9-step biosynthetic pathway that differ only at the final two steps after synthesis of prephenate.

Tryptophan is also produced from these two initial molecules, but its synthesis is an 11step pathway. Tyrosine may also be synthesized from phenylalanine, in a reaction catalyzed by phenylalanine hydroxylase. Alanine, valine, and leucine are all biosynthetic products of pyruvate, the final product of glycolysis. Aspartate is formed from oxaloacetate, an intermediate of the citric acid cycle. Asparagine, methionine, threonine, and lysine are each produced by the conversion of aspartate. Isoleucine is formed from threonine. A complex 9-step pathway results in the production of histidine from 5-phosphoribosyl-l-pyrophosphate, an activated sugar.

Amino acids in excess of the protein synthesis needs of the cell cannot be stored, and are instead degraded to provide intermediates for the major metabolic pathways of the cell (for review see Stryer, L. Biochemistry 3 rd ed. Ch. 21 "Amino Acid Degradation and the Urea Cycle" p. 495-516 (1988)). Although the cell is able to convert unwanted amino acids into useful metabolic intermediates, amino acid production is costly in terms of energy, precursor molecules, and the enzymes necessary to synthesize them.

Thus it is not surprising that amino acid biosynthesis is regulated by feedback inhibition, in which the presence of a particular amino acid serves to slow or entirely stop its own production (for overview of feedback mechanisms in amino acid biosynthetic pathways, see Stryer, L. Biochemistry, 3 rd ed. Ch. 24: "Biosynthesis of Amino Acids and Heme" p.

575-600 (1988)). Thus, the output of any particular amino acid is limited by the amount of that amino acid present in the cell.

B. Vitamin, Cofactor, and Nutraceutical Metabolism and Uses Vitamins, cofactors, and nutraceuticals comprise another group of molecules which the higher animals have lost the ability to synthesize and so must ingest, although WO 01/00843 PCT/IB00/00923 they are readily synthesized by other organisms, such as bacteria. These molecules are either bioactive substances themselves, or are precursors of biologically active substances which may serve as electron carriers or intermediates in a variety of metabolic pathways. Aside from their nutritive value, these compounds also have significant industrial value as coloring agents, antioxidants, and catalysts or other processing aids. (For an overview of the structure, activity, and industrial applications of these compounds, see, for example, Ullman's Encyclopedia of Industrial Chemistry, "Vitamins" vol. A27, p. 443-613, VCH: Weinheim, 1996.) The term "vitamin" is artrecognized, and includes nutrients which are required by an organism for normal functioning, but which that organism cannot synthesize by itself. The group of vitamins may encompass cofactors and nutraceutical compounds. The language "cofactor" includes nonproteinaceous compounds required for a normal enzymatic activity to occur. Such compounds may be organic or inorganic; the cofactor molecules of the invention are preferably organic. The term "nutraceutical" includes dietary supplements having health benefits in plants and animals, particularly humans. Examples of such molecules are vitamins, antioxidants, and also certain lipids polyunsaturated fatty acids).

The biosynthesis of these molecules in organisms capable of producing them, such as bacteria, has been largely characterized (Ullman's Encyclopedia of Industrial Chemistry, "Vitamins" vol. A27, p. 443-613, VCH: Weinheim, 1996; Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley Sons; Ong, Niki, E. Packer, L. (1995) "Nutrition, Lipids, Health, and Disease" Proceedings of the UNESCO/Confederation of Scientific and Technological Associations in Malaysia, and the Society for Free Radical Research Asia, held Sept.

1-3, 1994 at Penang, Malaysia, AOCS Press: Champaign, IL X, 374 S).

Thiamin (vitamin B is produced by the chemical coupling of pyrimidine and thiazole moieties. Riboflavin (vitamin B 2 is synthesized from (GTP) and ribose-5'-phosphate. Riboflavin, in turn, is utilized for the synthesis of flavin mononucleotide (FMN) and flavin adenine dinucleotide (FAD). The family of compounds collectively termed 'vitamin B 6 pyridoxine, pyridoxamine, pyridoxaand the commercially used pyridoxin hydrochloride) are all derivatives of the common structural unit, 5-hydroxy-6-methylpyridine. Pantothenate (pantothenic WO 01/00843 PCT/IB00/00923 -16acid, (R)-(+)-N-(2,4-dihydroxy-3,3-dimethyl- 1-oxobutyl)-P-alanine) can be produced either by chemical synthesis or by fermentation. The final steps in pantothenate biosynthesis consist of the ATP-driven condensation of p-alanine and pantoic acid. The enzymes responsible for the biosynthesis steps for the conversion to pantoic acid, to palanine and for the condensation to panthotenic acid are known. The metabolically active form of pantothenate is Coenzyme A, for which the biosynthesis proceeds in enzymatic steps. Pantothenate, pyridoxal-5'-phosphate, cysteine and ATP are the precursors of Coenzyme A. These enzymes not only catalyze the formation of panthothante, but also the production of(R)-pantoic acid, (R)-pantolacton, panthenol (provitamin B 5 pantetheine (and its derivatives) and coenzyme A.

Biotin biosynthesis from the precursor molecule pimeloyl-CoA in microorganisms has been studied in detail and several of the genes involved have been identified. Many of the corresponding proteins have been found to also be involved in Fe-cluster synthesis and are members of the nifS class of proteins. Lipoic acid is derived from octanoic acid, and serves as a coenzyme in energy metabolism, where it becomes part of the pyruvate dehydrogenase complex and the a-ketoglutarate dehydrogenase complex. The folates are a group of substances which are all derivatives of folic acid, which is turn is derived from L-glutamic acid, p-amino-benzoic acid and 6methylpterin. The biosynthesis of folic acid and its derivatives, starting from the metabolism intermediates guanosine-5'-triphosphate (GTP), L-glutamic acid and pamino-benzoic acid has been studied in detail in certain microorganisms.

Corrinoids (such as the cobalamines and particularly vitamin B1 2 and porphyrines belong to a group of chemicals characterized by a tetrapyrole ring system.

The biosynthesis of vitamin B12 is sufficiently complex that it has not yet been completely characterized, but many of the enzymes and substrates involved are now known. Nicotinic acid (nicotinate), and nicotinamide are pyridine derivatives which are also termed 'niacin'. Niacin is the precursor of the important coenzymes NAD (nicotinamide adenine dinucleotide) and NADP (nicotinamide adenine dinucleotide phosphate) and their reduced forms.

The large-scale production of these compounds has largely relied on cell-free chemical syntheses, though some of these chemicals have also been produced by largescale culture of microorganisms, such as riboflavin, Vitamin B 6 pantothenate, and WO 01/00843 PCT/IB00/00923 -17biotin. Only Vitamin Bi 2 is produced solely by fermentation, due to the complexity of its synthesis. In vitro methodologies require significant inputs of materials and time, often at great cost.

C. Purine, Pyrimidine, Nucleoside and Nucleotide Metabolism and Uses Purine and pyrimidine metabolism genes and their corresponding proteins are important targets for the therapy of tumor diseases and viral infections. The language "purine" or "pyrimidine" includes the nitrogenous bases which are constituents of nucleic acids, co-enzymes, and nucleotides. The term "nucleotide" includes the basic structural units of nucleic acid molecules, which are comprised of a nitrogenous base, a pentose sugar (in the case of RNA, the sugar is ribose; in the case of DNA, the sugar is D-deoxyribose), and phosphoric acid. The language "nucleoside" includes molecules which serve as precursors to nucleotides, but which are lacking the phosphoric acid moiety that nucleotides possess. By inhibiting the biosynthesis of these molecules, or their mobilization to form nucleic acid molecules, it is possible to inhibit RNA and DNA synthesis; by inhibiting this activity in a fashion targeted to cancerous cells, the ability of tumor cells to divide and replicate may be inhibited. Additionally, there are nucleotides which do not form nucleic acid molecules, but rather serve as energy stores AMP) or as coenzymes FAD and NAD).

Several publications have described the use of these chemicals for these medical indications, by influencing purine and/or pyrimidine metabolism Christopherson, R.I. and Lyons, S.D. (1990) "Potent inhibitors of de novo pyrimidine and purine biosynthesis as chemotherapeutic agents." Med. Res. Reviews 10: 505-548). Studies of enzymes involved in purine and pyrimidine metabolism have been focused on the development of new drugs which can be used, for example, as immunosuppressants or anti-proliferants (Smith, (1995) "Enzymes in nucleotide synthesis." Curr. Opin.

Struct. Biol. 5: 752-757; (1995) Biochem Soc. Transact. 23: 877-902). However, purine and pyrimidine bases, nucleosides and nucleotides have other utilities: as intermediates in the biosynthesis of several fine chemicals thiamine, S-adenosyl-methionine, folates, or riboflavin), as energy carriers for the cell ATP or GTP), and for chemicals themselves, commonly used as flavor enhancers IMP or GMP) or for several medicinal applications (see, for example, Kuninaka, A. (1996) Nucleotides and WO 01/00843 PCT/IB00/00923 18- Related Compounds in Biotechnology vol. 6, Rehm et al., eds. VCH: Weinheim, p. 561- 612). Also, enzymes involved in purine, pyrimidine, nucleoside, or nucleotide metabolism are increasingly serving as targets against which chemicals for crop protection, including fungicides, herbicides and insecticides, are developed.

The metabolism of these compounds in bacteria has been characterized (for reviews see, for example, Zalkin, H. and Dixon, J.E. (1992) "de novo purine nucleotide biosynthesis", in: Progress in Nucleic Acid Research and Molecular Biology, vol. 42, Academic Press:, p. 259-287; and Michal, G. (1999) "Nucleotides and Nucleosides", Chapter 8 in: Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, Wiley: New York). Purine metabolism has been the subject of intensive research, and is essential to the normal functioning of the cell. Impaired purine metabolism in higher animals can cause severe disease, such as gout. Purine nucleotides are synthesized from in a series of steps through the intermediate compound phosphate (IMP), resulting in the production of guanosine-5'-monophosphate (GMP) or adenosine-5'-monophosphate (AMP), from which the triphosphate forms utilized as nucleotides are readily formed. These compounds are also utilized as energy stores, so their degradation provides energy for many different biochemical processes in the cell.

Pyrimidine biosynthesis proceeds by the formation of uridine-5'-monophosphate (UMP) from ribose-5-phosphate. UMP, in turn, is converted to cytidine-5'-triphosphate (CTP).

The deoxy- forms of all of these nucleotides are produced in a one step reduction reaction from the diphosphate ribose form of the nucleotide to the diphosphate deoxyribose form of the nucleotide. Upon phosphorylation, these molecules are able to participate in DNA synthesis.

D. Trehalose Metabolism and Uses Trehalose consists of two glucose molecules, bound in a, a-1,l linkage. It is commonly used in the food industry as a sweetener, an additive for dried or frozen foods, and in beverages. However, it also has applications in the pharmaceutical, cosmetics and biotechnology industries (see, for example, Nishimoto et al., (1998) U.S.

Patent No. 5,759,610; Singer, M.A. and Lindquist, S. (1998) Trends Biotech. 16: 460- 467; Paiva, C.L.A. and Panek, A.D. (1996) Biotech. Ann. Rev. 2: 293-314; and Shiosaka, M. (1997) J. Japan 172: 97-102). Trehalose is produced by enzymes from WO 01/00843 PCT/IB00/00923 -19many microorganisms and is naturally released into the surrounding medium, from which it can be collected using methods known in the art.

II. Elements and Methods of the Invention The present invention is based, at least in part, on the discovery of novel molecules, referred to herein as MP nucleic acid and protein molecules, which play a role in or function in one or more cellular metabolic pathways. In one embodiment, the MP molecules catalyze an enzymatic reaction involving one or more amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathways. In a preferred embodiment, the activity of the MP molecules of the present invention in one or more C. glutamicum metabolic pathways for amino acids, vitamins, cofactors, nutraceuticals, nucleotides, nucleosides or trehalose has an impact on the production of a desired fine chemical by this organism. In a particularly preferred embodiment, the MP molecules of the invention are modulated in activity, such that the C. glutamicum metabolic pathways in which the MP proteins of the invention are involved are modulated in efficiency or output, which either directly or indirectly modulates the production or efficiency of production of a desired fine chemical by C glutamicum.

The language, "MP protein" or "MP polypeptide" includes proteins which play a role in, catalyze an enzymatic reaction, in one or more amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside or trehalose metabolic pathways.

Examples of MP proteins include those encoded by the MP genes set forth in Table 1 and by the odd-numbered SEQ ID NOs. The terms "MP gene" or "MP nucleic acid sequence" include nucleic acid sequences encoding an MP protein, which consist of a coding region and also corresponding untranslated 5' and 3' sequence regions.

Examples of MP genes include those set forth in Table 1. The terms "production" or "productivity" are art-recognized and include the concentration of the fermentation product (for example, the desired fine chemical) formed within a given time and a given fermentation volume kg product per hour per liter). The term "efficiency of production" includes the time required for a particular level of production to be achieved (for example, how long it takes for the cell to attain a particular rate of output of a fine chemical). The term "yield" or "product/carbon yield" is art-recognized and includes WO 01/00843 PCT/IBOO/00923 the efficiency of the conversion of the carbon source into the product fine chemical). This is generally written as, for example, kg product per kg carbon source.

By increasing the yield or production of the compound, the quantity of recovered molecules, or of useful recovered molecules of that compound in a given amount of culture over a given amount of time is increased. The terms "biosynthesis" or a "biosynthetic pathway" are art-recognized and include the synthesis of a compound, preferably an organic compound, by a cell from intermediate compounds in what may be a multistep and highly regulated process. The terms "degradation" or a "degradation pathway" are art-recognized and include the breakdown of a compound, preferably an organic compound, by a cell to degradation products (generally speaking, smaller or less complex molecules) in what may be a multistep and highly regulated process. The language "metabolism" is art-recognized and includes the totality of the biochemical reactions that take place in an organism. The metabolism of a particular compound, then, the metabolism of an amino acid such as glycine) comprises the overall biosynthetic, modification, and degradation pathways in the cell related to this compound.

In another embodiment, the MP molecules of the invention are capable of modulating the production of a desired molecule, such as a fine chemical, in a microorganism such as C. glutamicum. Using recombinant genetic techniques, one or more of the biosynthetic or degradative enzymes of the invention for amino acids, vitamins, cofactors, nutraceuticals, nucleotides, nucleosides, or trehalose may be manipulated such that its function is modulated. For example, a biosynthetic enzyme may be improved in efficiency, or its allosteric control region destroyed such that feedback inhibition of production of the compound is prevented. Similarly, a degradative enzyme may be deleted or modified by substitution, deletion, or addition such that its degradative activity is lessened for the desired compound without impairing the viability of the cell. In each case, the overall yield or rate of production of one of these desired fine chemicals may be increased.

It is also possible that such alterations in the protein and nucleotide molecules of the invention may improve the production of other fine chemicals besides the amino acids, vitamins, cofactors, nutraceuticals, nucleotides, nucleosides, and trehalose.

Metabolism of any one compound is necessarily intertwined with other biosynthetic and WO 01/00843 PCT/IBOO/00923 -21 degradative pathways within the cell, and necessary cofactors, intermediates, or substrates in one pathway are likely supplied or limited by another such pathway.

Therefore, by modulating the activity of one or more of the proteins of the invention, the production or efficiency of activity of another fine chemical biosynthetic or degradative pathway may be impacted. For example, amino acids serve as the structural units of all proteins, yet may be present intracellularly in levels which are limiting for protein synthesis; therefore, by increasing the efficiency of production or the yields of one or more amino acids within the cell, proteins, such as biosynthetic or degradative proteins, may be more readily synthesized. Likewise, an alteration in a metabolic pathway enzyme such that a particular side reaction becomes more or less favored may result in the over- or under-production of one or more compounds which are utilized as intermediates or substrates for the production of a desired fine chemical.

The isolated nucleic acid sequences of the invention are contained within the genome of a Corynebacterium glutamicum strain available through the American Type Culture Collection, given designation ATCC 13032. The nucleotide sequence of the isolated C. glutamicum MP DNAs and the predicted amino acid sequenceg of the C.

glutamicum MP proteins are shown in the Sequence Listing as odd-numbered SEQ ID NOs and even-numbered SEQ ID NOs, respectively. Computational analyses were performed which classified and/or identified these nucleotide sequences as sequences which encode metabolic pathway proteins.

The present invention also pertains to proteins which have an amino acid sequence which is substantially homologous to an amino acid sequence of the invention the sequence of an even-numbered SEQ ID NO of the Sequence Listing). As used herein, a protein which has an amino acid sequence which is substantially homologous to a selected amino acid sequence is least about 50% homologous to the selected amino acid sequence, the entire selected amino acid sequence. A protein which has an amino acid sequence which is substantially homologous to a selected amino acid sequence can also be least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-80%, 80-90%, or 90-95%, and most preferably at least about 96%, 97%, 98%, 99% or more homologous to the selected amino acid sequence.

The MP protein or a biologically active portion or fragment thereof of the invention can catalyze an enzymatic reaction in one or more amino acid, vitamin, WO 01/00843 PCT/IBOO/00923 -22cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathways, or have one or more of the activities set forth in Table 1.

Various aspects of the invention are described in further detail in the following subsections: A. Isolated Nucleic Acid Molecules One aspect of the invention pertains to isolated nucleic acid molecules that encode MP polypeptides or biologically active portions thereof, as well as nucleic acid fragments sufficient for use as hybridization probes or primers for the identification or amplification of MP-encoding nucleic acid MP DNA). As used herein, the term "nucleic acid molecule" is intended to include DNA molecules cDNA or genomic DNA) and RNA molecules mRNA) and analogs of the DNA or RNA generated using nucleotide analogs. This term also encompasses untranslated sequence located at both the 3' and 5' ends of the coding region of the gene: at least about 100 nucleotides of sequence upstream from the 5' end of the coding region and at least about nucleotides of sequence downstream from the 3'end of the coding region of the gene.

The nucleic acid molecule can be single-stranded or double-stranded, but preferably is double-stranded DNA. An "isolated" nucleic acid molecule is one which is separated from other nucleic acid molecules which are present in the natural source of the nucleic acid. Preferably, an "isolated" nucleic acid is free of sequences which naturally flank the nucleic acid sequences located at the 5' and 3' ends of the nucleic acid) in the genomic DNA of the organism from which the nucleic acid is derived. For example, in various embodiments, the isolated MP nucleic acid molecule can contain less than about kb, 4kb, 3kb, 2kb, 1 kb, 0.5 kb or 0.1 kb of nucleotide sequences which naturally flank the nucleic acid molecule in genomic DNA of the cell from which the nucleic acid is derived a C. glutamicum cell). Moreover, an "isolated" nucleic acid molecule, such as a DNA molecule, can be substantially free of other cellular material, or culture medium when produced by recombinant techniques, or chemical precursors or other chemicals when chemically synthesized.

A nucleic acid molecule of the present invention, a nucleic acid molecule having a nucleotide sequence of an odd-numbered SEQ ID NO of the Sequence Listing, or a portion thereof, can be isolated using standard molecular biology techniques and the WO 01/00843 PCT/IB00/00923 -23sequence information provided herein. For example, a C. glutamicum MP DNA can be isolated from a C. glutamicum library using all or portion of one of the odd-numbered SEQ ID NO sequences of the Sequence Listing as a hybridization probe and standard hybridization techniques as described in Sambrook, Fritsh, E. and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989).

Moreover, a nucleic acid molecule encompassing all or a portion of one of the nucleic acid sequences of the invention an odd-numbered SEQ ID NO:) can be isolated by the polymerase chain reaction using oligonucleotide primers designed based upon this sequence a nucleic acid molecule encompassing all or a portion of one of the nucleic acid sequences of the invention an odd-numbered SEQ ID NO of the Sequence Listing) can be isolated by the polymerase chain reaction using oligonucleotide primers designed based upon this same sequence). For example, mRNA can be isolated from normal endothelial cells by the guanidinium-thiocyanate extraction procedure of Chirgwin et al. (1979) Biochemistry 18: 5294-5299) and DNA can be prepared using reverse transcriptase Moloney MLV reverse transcriptase, available from Gibco/BRL, Bethesda, MD; or AMV reverse transcriptase, available from Seikagaku America, Inc., St. Petersburg, FL). Synthetic oligonucleotide primers for polymerase chain reaction amplification can be designed based upon one of the nucleotide sequences shown in the Sequence Listing. A nucleic acid of the invention can be amplified using cDNA or, alternatively, genomic DNA, as a template and appropriate oligonucleotide primers according to standard PCR amplification techniques. The nucleic acid so amplified can be cloned into an appropriate vector and characterized by DNA sequence analysis. Furthermore, oligonucleotides corresponding to an MP nucleotide sequence can be prepared by standard synthetic techniques, e.g., using an automated DNA synthesizer.

In a preferred embodiment, an isolated nucleic acid molecule of the invention comprises one of the nucleotide sequences shown in the Sequence Listing. The nucleic acid sequences of the invention, as set forth in the Sequence Listing, correspond to the Corynebacterium glutamicum MP DNAs of the invention. This DNA comprises sequences encoding MP proteins the "coding region", indicated in each oddnumbered SEQ ID NO: sequence in the Sequence Listing), as well as 5' untranslated WO 01/00843 PCT/IB00/00923 -24sequences and 3' untranslated sequences, also indicated in each odd-numbered SEQ ID NO: in the Sequence Listing. Alternatively, the nucleic acid molecule can comprise only the coding region of any of the nucleic acid sequences of the Sequence Listing.

For the purposes of this application, it will be understood that each of the nucleic acid and amino acid sequences set forth in the Sequence Listing has an identifying RXA, RXN, RXS, or RXC number having the designation "RXA", "RXN", "RXS", or "RXC" followed by 5 digits RXA00007, RXN00023, RXS00116, or RXC00128). Each of the nucleic acid sequences comprises up to three parts: a 5' upstream region, a coding region, and a downstream region. Each of these three regions is identified by the same RXA, RXN, RXS, or RXC designation to eliminate confusion. The recitation "one of the odd-numbered sequences of the Sequence Listing", then, refers to any of the nucleic acid sequences in the Sequence Listing, which may also be distinguished by their differing RXA, RXN, RXS, or RXC designations. The coding region of each of these sequences is translated into a corresponding amino acid sequence, which is also set forth in the Sequence Listing, as an even-numbered SEQ ID NO: immediately following the corresponding nucleic acid sequence. For example, the coding region for RXA02229 is set forth in SEQ ID NO:1, while the amino acid sequence which it encodes is set forth as SEQ ID NO:2. The sequences of the nucleic acid molecules of the invention are identified by the same RXA, RXN, RXS, or RXC designations as the amino acid molecules which they encode, such that they can be readily correlated. For example, the amino acid sequences designated RXA02229, RX00351, RXS02970, and RXC02390 are translations of the coding regions of the nucleotide sequences of nucleic acid molecules RXA02229, RX00351, RXS02970, and RXC02390, respectively. The correspondence between the RXA, RXN, RXS, and RXC nucleotide and amino acid sequences of the invention and their assigned SEQ ID NOs is set forth in Table 1.

Several of the genes of the invention are "F-designated genes". An F-designated gene includes those genes set forth in Table 1 which have an in front of the RXA, RXN, RXS, or RXC designation. For example, SEQ ID NO:5, designated, as indicated on Table 1, as "F RXA01009", is an F-designated gene, as are SEQ ID NOs: 73, 75, and 77 (designated on Table 1 as "F RXA00007", "F RXA00364", and "F RXA00367", respectively).

WO 01/00843 PCT/IBOO/00923 In one embodiment, the nucleic acid molecules of the present invention are not intended to include C. glutamicum those compiled in Table 2. In the case of the dapD gene, a sequence for this gene was published in Wehrmann, et al. (1998) J.

Bacteriol. 180(12): 3159-3165. However, the sequence obtained by the inventors of the present application is significantly longer than the published version. It is believed that the published version relied on an incorrect start codon, and thus represents only a fragment of the actual coding region.

In another preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleic acid molecule which is a complement of one of the nucleotide sequences of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing), or a portion thereof. A nucleic acid molecule which is complementary to one of the nucleotide sequences of the invention is one which is sufficiently complementary to one of the nucleotide sequences shown in the Sequence Listing the sequence of an odd-numbered SEQ ID NO:) such that it can hybridize to one of the nucleotide sequences of the invention, thereby forming a stable duplex.

In still another preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleotide sequence which is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to a nucleotide sequence of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing), or a portion thereof. Ranges and identity values intermediate to the above-recited ranges, 70-90% identical or 80-95% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included. In an additional preferred embodiment, an isolated nucleic acid molecule of the invention comprises a nucleotide sequence which hybridizes, hybridizes under stringent conditions, to one of the nucleotide sequences of the invention, or a portion thereof.

Moreover, the nucleic acid molecule of the invention can comprise only a portion of the coding region of the sequence of one of the odd-numbered SEQ ID NOs WO 01/00843 PCT/IB00/00923 -26of the Sequence Listing, for example a fragment which can be used as a probe or primer or a fragment encoding a biologically active portion of an MP protein. The nucleotide sequences determined from the cloning of the MP genes from C. glutamicum allows for the generation of probes and primers designed for use in identifying and/or cloning MP homologues in other cell types and organisms, as well as MP homologues from other Corynebacteria or related species. The probe/primer typically comprises substantially purified oligonucleotide. The oligonucleotide typically comprises a region of nucleotide sequence that hybridizes under stringent conditions to at least about 12, preferably about more preferably about 40, 50 or 75 consecutive nucleotides of a sense strand of one of the nucleotide sequences of the invention a sequence of one of the oddnumbered SEQ ID NOs of the Sequence Listing), an anti-sense sequence of one of these sequences, or naturally occurring mutants thereof. Primers based on a nucleotide sequence of the invention can be used in PCR reactions to clone MP homologues.

Probes based on the MP nucleotide sequences can be used to detect transcripts or genomic sequences encoding the same or homologous proteins. In preferred embodiments, the probe further comprises a label group attached thereto, e.g. the label group can be a radioisotope, a fluorescent compound, an enzyme, or an enzyme cofactor. Such probes can be used as a part of a diagnostic test kit for identifying cells which misexpress an MP protein, such as by measuring a level of an MP-encoding nucleic acid in a sample of cells from a subject detecting MP mRNA levels or determining whether a genomic MP gene has been mutated or deleted.

In one embodiment, the nucleic acid molecule of the invention encodes a protein or portion thereof which includes an amino acid sequence which is sufficiently homologous to an amino acid sequence of the invention a sequence of an evennumbered SEQ ID NO of the Sequence Listing) such that the protein or portion thereof maintains the ability to catalyze an enzymatic reaction in an amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathway. As used herein, the language "sufficiently homologous" refers to proteins or portions thereof which have amino acid sequences which include a minimum number of identical or equivalent an amino acid residue which has a similar side chain as an amino acid residue in a sequence of one of the even-numbered SEQ ID NOs of the Sequence Listing) amino acid residues to an amino acid sequence of the invention such that the WO 01/00843 PCT/IB00/00923 -27protein or portion thereof is able to catalyze an enzymatic reaction in a C. glutamicum amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside or trehalose metabolic pathway. Protein members of such metabolic pathways, as described herein, function to catalyze the biosynthesis or degradation of one or more of: amino acids, vitamins, cofactors, nutraceuticals, nucleotides, nucleosides, or trehalose. Examples of such activities are also described herein. Thus, "the function of an MP protein" contributes to the overall functioning of one or more such metabolic pathway and contributes, either directly or indirectly, to the yield, production, and/or efficiency of production of one or more fine chemicals. Examples of MP protein activities are set forth in Table 1.

In another embodiment, the protein is at least about 50-60%, preferably at least about 60-70%, and more preferably at least about 70-80%, 80-90%, 90-95%, and most preferably at least about 96%, 97%, 98%, 99% or more homologous to an entire amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing).

Portions of proteins encoded by the MP nucleic acid molecules of the invention are preferably biologically active portions of one of the MP proteins. As used herein, the term "biologically active portion of an MP protein" is intended to include a portion, a domain/motif, of an MP protein that catalyzes an enzymatic reaction in one or more C. glutamicum amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathways, or has an activity as set forth in Table 1. To determine whether an MP protein or a biologically active portion thereof can catalyze an enzymatic reaction in an amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathway, an assay of enzymatic activity may be performed. Such assay methods are well known to those of ordinary skill in the art, as detailed in Example 8 of the Exemplification.

Additional nucleic acid fragments encoding biologically active portions of an MP protein can be prepared by isolating a portion of one of the amino acid sequences of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing), expressing the encoded portion of the MP protein or peptide by recombinant expression in vitro) and assessing the activity of the encoded portion of the MP protein or peptide.

WO 01/00843 PCT/IB00/00923 -28- The invention further encompasses nucleic acid molecules that differ from one of the nucleotide sequences of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing) (and portions thereof) due to degeneracy of the genetic code and thus encode the same MP protein as that encoded by the nucleotide sequences of the invention. In another embodiment, an isolated nucleic acid molecule of the invention has a nucleotide sequence encoding a protein having an amino acid sequence shown in the Sequence Listing an even-numbered SEQ ID In a still further embodiment, the nucleic acid molecule of the invention encodes a full length C.

glutamicum protein which is substantially homologous to an amino acid sequence of the invention (encoded by an open reading frame shown in an odd-numbered SEQ ID NO: of the Sequence Listing).

It will be understood by one of ordinary skill in the art that in one embodiment the sequences of the invention are not meant to include the sequences of the prior art, such as those Genbank sequences set forth in Tables 2 or 4 which were available prior to the present invention. In one embodiment, the invention includes nucleotide and amino acid sequences having a percent identity to a nucleotide or amino acid sequence of the invention which is greater than that of a sequence of the prior art a Genbank sequence (or the protein encoded by such a sequence) set forth in Tables 2 or For example, the invention includes a nucleotide sequence which is greater than and/or at least 40% identical to the nucleotide sequence designated RXA00115 (SEQ ID NO:185), a nucleotide sequence which is greater than and/or at least identical to the nucleotide sequence designated RXA00131 (SEQ ID NO:991), and a nucleotide sequence which is greater than and/or at least 39% identical to the nucleotide sequence designated RXA00219 (SEQ ID NO:345). One of ordinary skill in the art would be able to calculate the lower threshold of percent identity for any given sequence of the invention by examining the GAP-calculated percent identity scores set forth in Table 4 for each of the three top hits for the given sequence, and by subtracting the highest GAP-calculated percent identity from 100 percent. One of ordinary skill in the art will also appreciate that nucleic acid and amino acid sequences having percent identities greater than the lower threshold so calculated at least 50%, 51%, 52%, 53%, 54%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, WO 01/00843 PCT/IBO0/00923 -29- 74%, 75%, 76%, 77%, 78%, 79%, or 80%, or 80%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 96%, 97%, 98%, 99% or more identical) are also encompassed by the invention.

In addition to the C. glutamicum MP nucleotide sequences set forth in the Sequence Listing as odd-numbered SEQ ID NOs, it will be appreciated by one of ordinary skill in the art that DNA sequence polymorphisms that lead to changes in the amino acid sequences of MP proteins may exist within a population the C.

glutamicum population). Such genetic polymorphism in the MP gene may exist among individuals within a population due to natural variation. As used herein, the terms "gene" and "recombinant gene" refer to nucleic acid molecules comprising an open reading frame encoding an MP protein, preferably a C. glutamicum MP protein. Such natural variations can typically result in 1-5% variance in the nucleotide sequence of the MP gene. Any and all such nucleotide variations and resulting amino acid polymorphisms in MP that are the result of natural variation and that do not alter the functional activity of MP proteins are intended to be within the scope of the invention.

Nucleic acid molecules corresponding to natural variants and non-C. glutamicum homologues of the C. glutamicum MP DNA of the invention can be isolated based on their homology to the C. glutamicum MP nucleic acid disclosed herein using the C.

glutamicum DNA, or a portion thereof, as a hybridization probe according to standard hybridization techniques under stringent hybridization conditions. Accordingly, in another embodiment, an isolated nucleic acid molecule of the invention is at least nucleotides in length and hybridizes under stringent conditions to the nucleic acid molecule comprising a nucleotide sequence of an odd-numbered SEQ ID NO: of the Sequence Listing. In other embodiments, the nucleic acid is at least 30, 50, 100, 250 or more nucleotides in length. As used herein, the term "hybridizes under stringent conditions" is intended to describe conditions for hybridization and washing under which nucleotide sequences at least 60% homologous to each other typically remain hybridized to each other. Preferably, the conditions are such that sequences at least about 65%, more preferably at least about 70%, and even more preferably at least about 75% or more homologous to each other typically remain hybridized to each other. Such stringent conditions are known to one of ordinary skill in the art and can be found in Current Protocols in Molecular Biology, John Wiley Sons, N.Y. (1989), 6.3.1-6.3.6.

WO 01/00843 PCTIBO00/00923 A preferred, non-limiting example of stringent hybridization conditions are hybridization in 6X sodium chloride/sodium citrate (SSC) at about 45°C, followed by one or more washes in 0.2 X SSC, 0.1% SDS at 50-65 0 C. Preferably, an isolated nucleic acid molecule of the invention that hybridizes under stringent conditions to a nucleotide sequence of the invention corresponds to a naturally-occurring nucleic acid molecule. As used herein, a "naturally-occurring" nucleic acid molecule refers to an RNA or DNA molecule having a nucleotide sequence that occurs in nature encodes a natural protein). In one embodiment, the nucleic acid encodes a natural C.

glutamicum MP protein.

In addition to naturally-occurring variants of the MP sequence that may exist in the population, one of ordinary skill in the art will further appreciate that changes can be introduced by mutation into a nucleotide sequence of the invention, thereby leading to changes in the amino acid sequence of the encoded MP protein, without altering the functional ability of the MP protein. For example, nucleotide substitutions leading to amino acid substitutions at "non-essential" amino acid residues can be made in a nucleotide sequence of the invention. A "non-essential" amino acid residue is a residue that can be altered from the wild-type sequence of one of the MP proteins an evennumbered SEQ ID NO: of the Sequence Listing) without altering the activity of said MP protein, whereas an "essential" amino acid residue is required for MP protein activity.

Other amino acid residues, however, those that are not conserved or only semiconserved in the domain having MP activity) may not be essential for activity and thus are likely to be amenable to alteration without altering MP activity.

Accordingly, another aspect of the invention pertains to nucleic acid molecules encoding MP proteins that contain changes in amino acid residues that are not essential for MP activity. Such MP proteins differ in amino acid sequence from a sequence of an even-numbered SEQ ID NO: of the Sequence Listing yet retain at least one of the MP activities described herein. In one embodiment, the isolated nucleic acid molecule comprises a nucleotide sequence encoding a protein, wherein the protein comprises an amino acid sequence at least about 50% homologous to an amino acid sequence of the invention and is capable of catalyzing an enzymatic reaction in an amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathway, or has one or more activities set forth in Table 1. Preferably, the protein encoded by the nucleic WO 01/00843 PCT/IB00/00923 -31 acid molecule is at least about 50-60% homologous to the amino acid sequence of one of the odd-numbered SEQ ID NOs of the Sequence Listing, more preferably at least about 60-70% homologous to one of these sequences, even more preferably at least about 80-90%, 90-95% homologous to one of these sequences, and most preferably at least about 96%, 97%, 98%, or 99% homologous to one of the amino acid sequences of the invention.

To determine the percent homology of two amino acid sequences one of the amino acid sequences of the invention and a mutant form thereof) or of two nucleic acids, the sequences are aligned for optimal comparison purposes gaps can be introduced in the sequence of one protein or nucleic acid for optimal alignment with the other protein or nucleic acid). The amino acid residues or nucleotides at corresponding amino acid positions or nucleotide positions are then compared. When a position in one sequence one of the amino acid sequences of the invention) is occupied by the same amino acid residue or nucleotide as the corresponding position in the other sequence a mutant form of the amino acid sequence), then the molecules are homologous at that position as used herein amino acid or nucleic acid "homology" is equivalent to amino acid or nucleic acid "identity"). The percent homology between the two sequences is a function of the number of identical positions shared by the sequences homology of identical positions/total of positions x 100).

An isolated nucleic acid molecule encoding an MP protein homologous to a protein sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) can be created by introducing one or more nucleotide substitutions, additions or deletions into a nucleotide sequence of the invention such that one or more amino acid substitutions, additions or deletions are introduced into the encoded protein. Mutations can be introduced into one of the nucleotide sequences of the invention by standard techniques, such as site-directed mutagenesis and PCRmediated mutagenesis. Preferably, conservative amino acid substitutions are made at one or more predicted non-essential amino acid residues. A "conservative amino acid substitution" is one in which the amino acid residue is replaced with an amino acid residue having a similar side chain. Families of amino acid residues having similar side chains have been defined in the art. These families include amino acids with basic side chains lysine, arginine, histidine), acidic side chains aspartic acid, glutamic WO 01/00843 PCT/IB00/00923 -32acid), uncharged polar side chains glycine, asparagine, glutamine, serine, threonine, tyrosine, cysteine), nonpolar side chains alanine, valine, leucine, isoleucine, proline, phenylalanine, methionine, tryptophan), beta-branched side chains threonine, valine, isoleucine) and aromatic side chains tyrosine, phenylalanine, tryptophan, histidine). Thus, a predicted nonessential amino acid residue in an MP protein is preferably replaced with another amino acid residue from the same side chain family. Alternatively, in another embodiment, mutations can be introduced randomly along all or part of an MP coding sequence, such as by saturation mutagenesis, and the resultant mutants can be screened for an MP activity described herein to identify mutants that retain MP activity. Following mutagenesis of the nucleotide sequence of one of the odd-numbered SEQ ID NOs of the Sequence Listing, the encoded protein can be expressed recombinantly and the activity of the protein can be determined using, for example, assays described herein (see Example 8 of the Exemplification).

In addition to the nucleic acid molecules encoding MP proteins described above, another aspect of the invention pertains to isolated nucleic acid molecules which are antisense thereto. An "antisense" nucleic acid comprises a nucleotide sequence which is complementary to a "sense" nucleic acid encoding a protein, complementary to the coding strand of a double-stranded DNA molecule or complementary to an mRNA sequence. Accordingly, an antisense nucleic acid can hydrogen bond to a sense nucleic acid. The antisense nucleic acid can be complementary to an entire MP coding strand, or to only a portion thereof. In one embodiment, an antisense nucleic acid molecule is antisense to a "coding region" of the coding strand of a nucleotide sequence encoding an MP protein. The term "coding region" refers to the region of the nucleotide sequence comprising codons which are translated into amino acid residues the entire coding region of SEQ ID NO. 1 (RXA02229) comprises nucleotides 1 to 825). In another embodiment, the antisense nucleic acid molecule is antisense to a "noncoding region" of the coding strand of a nucleotide sequence encoding MP. The term "noncoding region" refers to 5' and 3' sequences which flank the coding region that are not translated into amino acids also referred to as 5' and 3' untranslated regions).

Given the coding strand sequences encoding MP disclosed herein the sequences set forth as odd-numbered SEQ ID NOs in the Sequence Listing), antisense WO 01/00843 PCT/IB00/00923 -33nucleic acids of the invention can be designed according to the rules of Watson and Crick base pairing. The antisense nucleic acid molecule can be complementary to the entire coding region of MP mRNA, but more preferably is an oligonucleotide which is antisense to only a portion of the coding or noncoding region of MP mRNA. For example, the antisense oligonucleotide can be complementary to the region surrounding the translation start site of MP mRNA. An antisense oligonucleotide can be, for example, about 5, 10, 15, 20, 25, 30, 35, 40, 45 or 50 nucleotides in length. An antisense nucleic acid of the invention can be constructed using chemical synthesis and enzymatic ligation reactions using procedures known in the art. For example, an antisense nucleic acid an antisense oligonucleotide) can be chemically synthesized using naturally occurring nucleotides or variously modified nucleotides designed to increase the biological stability of the molecules or to increase the physical stability of the duplex formed between the antisense and sense nucleic acids, phosphorothioate derivatives and acridine substituted nucleotides can be used. Examples of modified nucleotides which can be used to generate the antisense nucleic acid include fluorouracil, 5-bromouracil, 5-chlorouracil, 5-iodouracil, hypoxanthine, xanthine, 4acetylcytosine, 5-(carboxyhydroxylmethyl) uracil, 5-carboxymethylaminomethyl-2thiouridine, 5-carboxymethylaminomethyluracil, dihydrouracil, beta-Dgalactosylqueosine, inosine, N6-isopentenyladenine, 1 -methylguanine, 1 -methylinosine, 2,2-dimethylguanine, 2-methyladenine, 2-methylguanine, 3-methylcytosine, methylcytosine, N6-adenine, 7-methylguanine, 5-methylaminomethyluracil, methoxyaminomethyl-2-thiouracil, beta-D-mannosylqueosine, methoxycarboxymethyluracil, 5-methoxyuracil, 2-methylthio-N6-isopentenyladenine, acid wybutoxosine, pseudouracil, queosine, 2-thiocytosine, methyl-2-thiouracil, 2-thiouracil, 4-thiouracil, 5-methyluracil, uracil-5- oxyacetic acid methylester, uracil-5-oxyacetic acid 5-methyl-2-thiouracil, 3-(3-amino-3-N-2carboxypropyl) uracil, (acp3)w, and 2,6-diaminopurine. Alternatively, the antisense nucleic acid can be produced biologically using an expression vector into which a nucleic acid has been subcloned in an antisense orientation RNA transcribed from the inserted nucleic acid will be of an antisense orientation to a target nucleic acid of interest, described further in the following subsection).

WO 01/00843 PCT/IB00/00923 -34- The antisense nucleic acid molecules of the invention are typically administered to a cell or generated in situ such that they hybridize with or bind to cellular mRNA and/or genomic DNA encoding an MCT protein to thereby inhibit expression of the protein, by inhibiting transcription and/or translation. The hybridization can be by conventional nucleotide complementarity to form a stable duplex, or, for example, in the case of an antisense nucleic acid molecule which binds to DNA duplexes, through specific interactions in the major groove of the double helix. The antisense molecule can be modified such that it specifically binds to a receptor or an antigen expressed on a selected cell surface, by linking the antisense nucleic acid molecule to a peptide or an antibody which binds to a cell surface receptor or antigen. The antisense nucleic acid molecule can also be delivered to cells using the vectors described herein. To achieve sufficient intracellular concentrations of the antisense molecules, vector constructs in which the antisense nucleic acid molecule is placed under the control of a strong prokaryotic, viral, or eukaryotic promoter are preferred.

In yet another embodiment, the antisense nucleic acid molecule of the invention is an a-anomeric nucleic acid molecule. An a-anomeric nucleic acid molecule forms specific double-stranded hybrids with complementary RNA in which, contrary to the usual 3-units, the strands run parallel to each other (Gaultier et al. (1987) Nucleic Acids.

Res. 15:6625-6641). The antisense nucleic acid molecule can also comprise a 2'-omethylribonucleotide (Inoue et al. (1987) Nucleic Acids Res. 15:6131-6148) or a chimeric RNA-DNA analogue (Inoue et al. (1987) FEBS Lett. 215:327-330).

In still another embodiment, an antisense nucleic acid of the invention is a ribozyme. Ribozymes are catalytic RNA molecules with ribonuclease activity which are capable of cleaving a single-stranded nucleic acid, such as an mRNA, to which they have a complementary region. Thus, ribozymes hammerhead ribozymes (described in Haselhoff and Gerlach (1988) Nature 334:585-591)) can be used to catalytically cleave MP mRNA transcripts to thereby inhibit translation of MP mRNA.

A ribozyme having specificity for an MP-encoding nucleic acid can be designed based upon the nucleotide sequence of an MP DNA disclosed herein SEQ ID NO: 1 (RXA02229). For example, a derivative of a Tetrahymena L-19 IVS RNA can be constructed in which the nucleotide sequence of the active site is complementary to the nucleotide sequence to be cleaved in an MP-encoding mRNA. See, Cech et al.

WO 01/00843 PCT/IB00/00923 U.S. Patent No. 4,987,071 and Cech et al. U.S. Patent No. 5,116,742. Alternatively, MP mRNA can be used to select a catalytic RNA having a specific ribonuclease activity from a pool of RNA molecules. See, Bartel, D. and Szostak, J.W. (1993) Science 261:1411-1418.

Alternatively, MP gene expression can be inhibited by targeting nucleotide sequences complementary to the regulatory region of an MP nucleotide sequence an MP promoter and/or enhancers) to form triple helical structures that prevent transcription of an MP gene in target cells. See generally, Helene, C. (1991) Anticancer Drug Des. 6(6):569-84; Helene, C. et al. (1992) Ann. N. Y Acad. Sci. 660:27-36; and Maher, L.J. (1992) Bioassays 14(12):807-15.

B. Recombinant Expression Vectors and Host Cells Another aspect of the invention pertains to vectors, preferably expression vectors, containing a nucleic acid encoding an MP protein (or a portion thereof). As used herein, the term "vector" refers to a nucleic acid molecule capable of transporting another nucleic acid to which it has been linked. One type of vector is a "plasmid", which refers to a circular double stranded DNA loop into which additional DNA segments can be ligated. Another type of vector is a viral vector, wherein additional DNA segments can be ligated into the viral genome. Certain vectors are capable of autonomous replication in a host cell into which they are introduced bacterial vectors having a bacterial origin of replication and episomal mammalian vectors). Other vectors non-episomal mammalian vectors) are integrated into the genome of a host cell upon introduction into the host cell, and thereby are replicated along with the host genome. Moreover, certain vectors are capable of directing the expression of genes to which they are operatively linked. Such vectors are referred to herein as "expression vectors". In general, expression vectors of utility in recombinant DNA techniques are often in the form ofplasmids. In the present specification, "plasmid" and "vector" can be used interchangeably as the plasmid is the most commonly used form of vector.

However, the invention is intended to include such other forms of expression vectors, such as viral vectors replication defective retroviruses, adenoviruses and adenoassociated viruses), which serve equivalent functions.

WO 01/00843 PCT/IB00/00923 -36- The recombinant expression vectors of the invention comprise a nucleic acid of the invention in a form suitable for expression of the nucleic acid in a host cell, which means that the recombinant expression vectors include one or more regulatory sequences, selected on the basis of the host cells to be used for expression, which is operatively linked to the nucleic acid sequence to be expressed. Within a recombinant expression vector, "operably linked" is intended to mean that the nucleotide sequence of interest is linked to the regulatory sequence(s) in a manner which allows for expression of the nucleotide sequence in an in vitro transcription/translation system or in a host cell when the vector is introduced into the host cell). The term "regulatory sequence" is intended to include promoters, repressor binding sites, activator binding sites, enhancers and other expression control elements terminators, polyadenylation signals, or other elements of mRNA secondary structure). Such regulatory sequences are described, for example, in Goeddel; Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, CA (1990).

Regulatory sequences include those which direct constitutive expression of a nucleotide sequence in many types of host cell and those which direct expression of the nucleotide sequence only in certain host cells. Preferred regulatory sequences are, for example, promoters such as cos-, tac-, trp-, tet-, trp-tet-, Ipp-, lac-, Ipp-lac-, lacI q T7-, T5-, T3-, gal-, trc-, ara-, SP6-, arny, SP02, h-PR- or X PL, which are used preferably in bacteria.

Additional regulatory sequences are, for example, promoters from yeasts and fungi, such as ADC1, MFa, AC, P-60, CYC1, GAPDH, TEF, rp28, ADH, promoters from plants such as CaMV/35S, SSU, OCS, lib4, usp, STLSI, B33, nos or ubiquitin- or phaseolinpromoters. It is also possible to use artificial promoters. It will be appreciated by one of ordinary skill in the art that the design of the expression vector can depend on such factors as the choice of the host cell to be transformed, the level of expression of protein desired, etc. The expression vectors of the invention can be introduced into host cells to thereby produce proteins or peptides, including fusion proteins or peptides, encoded by nucleic acids as described herein MP proteins, mutant forms of MP proteins, fusion proteins, etc.).

The recombinant expression vectors of the invention can be designed for expression of MP proteins in prokaryotic or eukaryotic cells. For example, MP genes can be expressed in bacterial cells such as C. glutamicum, insect cells (using baculovirus WO 01/00843 PCT/IB00/00923 -37expression vectors), yeast and other fungal cells (see Romanos, M.A. et al. (1992) "Foreign gene expression in yeast: a review", Yeast 8: 423-488; van den Hondel, C.A.M.J.J. et al. (1991) "Heterologous gene expression in filamentous fungi" in: More Gene Manipulations in Fungi, J.W. Bennet L.L. Lasure, eds., p. 396-428: Academic Press: San Diego; and van den Hondel, C.A.M.J.J. Punt, P.J. (1991) "Gene transfer systems and vector development for filamentous fungi, in: Applied Molecular Genetics of Fungi, Peberdy, J.F. et al., eds., p. 1-28, Cambridge University Press: Cambridge), algae and multicellular plant cells (see Schmidt, R. and Willmitzer, L. (1988) High efficiency Agrobacterium tumefaciens -mediated transformation of Arabidopsis thaliana leaf and cotyledon explants" Plant Cell Rep.: 583-586), or mammalian cells.

Suitable host cells are discussed further in Goeddel, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, CA (1990). Alternatively, the recombinant expression vector can be transcribed and translated in vitro, for example using T7 promoter regulatory sequences and T7 polymerase.

Expression of proteins in prokaryotes is most often carried out with vectors containing constitutive or inducible promoters directing the expression of either fusion or non-fusion proteins. Fusion vectors add a number of amino acids to a protein encoded therein, usually to the amino terminus of the recombinant protein but also to the Cterminus or fused within suitable regions in the proteins. Such fusion vectors typically serve three purposes: 1) to increase expression of recombinant protein; 2) to increase the solubility of the recombinant protein; and 3) to aid in the purification of the recombinant protein by acting as a ligand in affinity purification. Often, in fusion expression vectors, a proteolytic cleavage site is introduced at the junction of the fusion moiety and the recombinant protein to enable separation of the recombinant protein from the fusion moiety subsequent to purification of the fusion protein. Such enzymes, and their cognate recognition sequences, include Factor Xa, thrombin and enterokinase.

Typical fusion expression vectors include pGEX (Pharmacia Biotech Inc; Smith, D.B. and Johnson, K.S. (1988) Gene 67:31-40), pMAL (New England Biolabs, Beverly, MA) and pRIT5 (Pharmacia, Piscataway, NJ) which fuse glutathione S-transferase (GST), maltose E binding protein, or protein A, respectively, to the target recombinant protein. In one embodiment, the coding sequence of the MP protein is cloned into a pGEX expression vector to create a vector encoding a fusion protein comprising, from WO 01/00843 PCT/IB00/00923 -38the N-terminus to the C-terminus, GST-thrombin cleavage site-X protein. The fusion protein can be purified by affinity chromatography using glutathione-agarose resin.

Recombinant MP protein unfused to GST can be recovered by cleavage of the fusion protein with thrombin.

Examples of suitable inducible non-fusion E. coli expression vectors include pTrc (Amann etal., (1988) Gene 69:301-315) pLG338, pACYC184, pBR322, pUC 18, pUC19, pKC30, pRep4, pHS1, pHS2, pPLc236, pMBL24, pLG200, pUR290, pIN- III113-B1, Xgtl 1, pBdCl, and pET Id (Studier et al., Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, California (1990) 60-89; and Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018).

Target gene expression from the pTrc vector relies on host RNA polymerase transcription from a hybrid trp-lac fusion promoter. Target gene expression from the pET 1 d vector relies on transcription from a T7 gnlO-lac fusion promoter mediated by a coexpressed viral RNA polymerase (T7 gnl). This viral polymerase is supplied by host strains BL21 (DE3) or HMS174(DE3) from a resident X prophage harboring a T7 gnl gene under the transcriptional control of the lacUV 5 promoter. For transformation of other varieties of bacteria, appropriate vectors may be selected. For example, the plasmids pIJl01, pIJ364, pIJ702 and pIJ361 are known to be useful in transforming Streptomyces, while plasmids pUB 10, pC194, or pBD214 are suited for transformation of Bacillus species. Several plasmids of use in the transfer of genetic information into Corynebacterium include pHM1519, pBL, pSA77, or pAJ667 (Pouwels et al., eds.

(1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018).

One strategy to maximize recombinant protein expression is to express the protein in a host bacteria with an impaired capacity to proteolytically cleave the recombinant protein (Gottesman, Gene Expression Technology: Methods in Enzymology 185, Academic Press, San Diego, California (1990) 119-128). Another strategy is to alter the nucleic acid sequence of the nucleic acid to be inserted into an expression vector so that the individual codons for each amino acid are those preferentially utilized in the bacterium chosen for expression, such as C. glutamicum (Wada et al. (1992) Nucleic Acids Res. 20:2111-2118). Such alteration of nucleic acid sequences of the invention can be carried out by standard DNA synthesis techniques.

WO 01/00843 PCT/IB00/00923 -39- In another embodiment, the MP protein expression vector is a yeast expression vector. Examples of vectors for expression in yeast S. cerevisiae include pYepSecl (Baldari, et al., (1987) Embo J 6:229-234),, 2 ji, pAG-1, Yep6, Yepl3, pEMBLYe23, pMFa (Kurjan and Herskowitz, (1982) Cell 30:933-943), pJRY88 (Schultz et al., (1987) Gene 54:113-123), and pYES2 (Invitrogen Corporation, San Diego, CA). Vectors and methods for the construction of vectors appropriate for use in other fungi, such as the filamentous fungi, include those detailed in: van den Hondel, C.A.M.J.J. Punt, P.J.

(1991) "Gene transfer systems and vector development for filamentous fungi, in: Applied Molecular Genetics of Fungi, J.F. Peberdy, et al., eds., p. 1-28, Cambridge University Press: Cambridge, and Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: New York (IBSN 0 444 904018).

Alternatively, the MP proteins of the invention can be expressed in insect cells using baculovirus expression vectors. Baculovirus vectors available for expression of proteins in cultured insect cells Sf 9 cells) include the pAc series (Smith et al.

(1983) Mol. Cell Biol. 3:2156-2165) and the pVL series (Lucklow and Summers (1989) Virology 170:31-39).

In another embodiment, the MP proteins of the invention may be expressed in unicellular plant cells (such as algae) or in plant cells from higher plants the spermatophytes, such as crop plants). Examples of plant expression vectors include those detailed in: Becker, Kemper, Schell, J. and Masterson, R. (1992) "New plant binary vectors with selectable markers located proximal to the left border", Plant Mol. Biol. 20: 1195-1197; and Bevan, M.W. (1984) "Binary Agrobacterium vectors for plant transformation", Nucl. Acid. Res. 12: 8711-8721, and include pLGV23, pGHlac+, pBIN19, pAK2004, and pDH51 (Pouwels et al., eds. (1985) Cloning Vectors. Elsevier: New York IBSN 0 444 904018).

In yet another embodiment, a nucleic acid of the invention is expressed in mammalian cells using a mammalian expression vector. Examples of mammalian expression vectors include pCDM8 (Seed, B. (1987) Nature 329:840) and pMT2PC (Kaufman et al. (1987) EMBOJ. 6:187-195). When used in mammalian cells, the expression vector's control functions are often provided by viral regulatory elements.

For example, commonly used promoters are derived from polyoma, Adenovirus 2, cytomegalovirus and Simian Virus 40. For other suitable expression systems for both WO 01/00843 PCT/IB00/00923 prokaryotic and eukaryotic cells see chapters 16 and 17 of Sambrook, Fritsh, E. F., and Maniatis, T. Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989.

In another embodiment, the recombinant mammalian expression vector is capable of directing expression of the nucleic acid preferentially in a particular cell type tissue-specific regulatory elements are used to express the nucleic acid). Tissuespecific regulatory elements are known in the art. Non-limiting examples of suitable tissue-specific promoters include the albumin promoter (liver-specific; Pinkert et al.

(1987) Genes Dev. 1:268-277), lymphoid-specific promoters (Calame and Eaton (1988) Adv. Immunol. 43:235-275), in particular promoters ofT cell receptors (Winoto and Baltimore (1989) EMBO J. 8:729-733) and immunoglobulins (Banerji et al. (1983) Cell 33:729-740; Queen and Baltimore (1983) Cell 33:741-748), neuron-specific promoters the neurofilament promoter; Byrne and Ruddle (1989) PNAS 86:5473-5477), pancreas-specific promoters (Edlund et al. (1985) Science 230:912-916), and mammary gland-specific promoters milk whey promoter; U.S. Patent No. 4,873,316 and European Application Publication No. 264,166). Developmentally-regulated promoters are also encompassed, for example the murine hox promoters (Kessel and Gruss (1990) Science 249:374-379) and the ao-fetoprotein promoter (Campes and Tilghman (1989) Genes Dev. 3:537-546).

The invention further provides a recombinant expression vector comprising a DNA molecule of the invention cloned into the expression vector in an antisense orientation. That is, the DNA molecule is operatively linked to a regulatory sequence in a manner which allows for expression (by transcription of the DNA molecule) of an RNA molecule which is antisense to MP mRNA. Regulatory sequences operatively linked to a nucleic acid cloned in the antisense orientation can be chosen which direct the continuous expression of the antisense RNA molecule in a variety of cell types, for instance viral promoters and/or enhancers, or regulatory sequences can be chosen which direct constitutive, tissue specific or cell type specific expression of antisense RNA.

The antisense expression vector can be in the form of a recombinant plasmid, phagemid or attenuated virus in which antisense nucleic acids are produced under the control of a high efficiency regulatory region, the activity of which can be determined by the cell WO 01/00843 PCT/IB00/00923 -41type into which the vector is introduced. For a discussion of the regulation of gene expression using antisense genes see Weintraub, H. et al., Antisense RNA as a molecular tool for genetic analysis, Reviews Trends in Genetics, Vol. 1(1) 1986.

Another aspect of the invention pertains to host cells into which a recombinant expression vector of the invention has been introduced. The terms "host cell" and "recombinant host cell" are used interchangeably herein. It is understood that such terms refer not only to the particular subject cell but to the progeny or potential progeny of such a cell. Because certain modifications may occur in succeeding generations due to either mutation or environmental influences, such progeny may not, in fact, be identical to the parent cell, but are still included within the scope of the term as used herein.

A host cell can be any prokaryotic or eukaryotic cell. For example, an MP protein can be expressed in bacterial cells such as C. glutamicum, insect cells, yeast or mammalian cells (such as Chinese hamster ovary cells (CHO) or COS cells). Other suitable host cells are known to those of ordinary skill in the art. Microorganisms related to Corynebacterium glutamicum which may be conveniently used as host cells for the nucleic acid and protein molecules of the invention are set forth in Table 3.

Vector DNA can be introduced into prokaryotic or eukaryotic cells via conventional transformation or transfection techniques. As used herein, the terms "transformation" and "transfection", "conjugation" and "transduction" are intended to refer to a variety of art-recognized techniques for introducing foreign nucleic acid linear DNA or RNA a linearized vector or a gene construct alone without a vector) or nucleic acid in the form of a vector a plasmid, phage, phasmid, phagemid, transposon or other DNA) into a host cell, including calcium phosphate or calcium chloride co-precipitation, DEAE-dextran-mediated transfection, lipofection, natural competence, chemical-mediated transfer, or electroporation. Suitable methods for transforming or transfecting host cells can be found in Sambrook, et al. (Molecular Cloning: A Laboratory Manual. 2nd, ed., Cold Spring Harbor Laboratory, Cold Spring Harbor Laboratory Press, Cold Spring Harbor, NY, 1989), and other laboratory manuals.

For stable transfection of mammalian cells, it is known that, depending upon the expression vector and transfection technique used, only a small fraction of cells may integrate the foreign DNA into their genome. In order to identify and select these WO 01/00843 PCT/IBOO/00923 -42integrants, a gene that encodes a selectable marker resistance to antibiotics) is generally introduced into the host cells along with the gene of interest. Preferred selectable markers include those which confer resistance to drugs, such as G418, hygromycin and methotrexate. Nucleic acid encoding a selectable marker can be introduced into a host cell on the same vector as that encoding an MP protein or can be introduced on a separate vector. Cells stably transfected with the introduced nucleic acid can be identified by drug selection cells that have incorporated the selectable marker gene will survive, while the other cells die).

To create a homologous recombinant microorganism, a vector is prepared which contains at least a portion of an MP gene into which a deletion, addition or substitution has been introduced to thereby alter, functionally disrupt, the MP gene. Preferably, this MP gene is a Corynebacterium glutamicum MP gene, but it can be a homologue from a related bacterium or even from a mammalian, yeast, or insect source. In a preferred embodiment, the vector is designed such that, upon homologous recombination, the endogenous MP gene is functionally disrupted no longer encodes a functional protein; also referred to as a "knock out" vector). Alternatively, the vector can be designed such .that, upon homologous recombination, the endogenous MP gene is mutated or otherwise altered but still encodes functional protein the upstream regulatory region can be altered to thereby alter the expression of the endogenous MP protein). In the homologous recombination vector, the altered portion of the MP gene is flanked at its 5' and 3' ends by additional nucleic acid of the MP gene to allow for homologous recombination to occur between the exogenous MP gene carried by the vector and an endogenous MP gene in a microorganism. The additional flanking MP nucleic acid is of sufficient length for successful homologous recombination with the endogenous gene. Typically, several kilobases of flanking DNA (both at the 5' and 3' ends) are included in the vector (see Thomas, and Capecchi, M.R. (1987) Cell 51: 503 for a description of homologous recombination vectors). The vector is introduced into a microorganism by electroporation) and cells in which the introduced MP gene has homologously recombined with the endogenous MP gene are selected, using art-known techniques.

In another embodiment, recombinant microorganisms can be produced which contain selected systems which allow for regulated expression of the introduced gene.

WO 01/00843 PCT/IBOO/00923 -43- For example, inclusion of an MP gene on a vector placing it under control of the lac operon permits expression of the MP gene only in the presence of IPTG. Such regulatory systems are well known in the art.

In another embodiment, an endogenous MP gene in a host cell is disrupted by homologous recombination or other genetic means known in the art) such that expression of its protein product does not occur. In another embodiment, an endogenous or introduced MP gene in a host cell has been altered by one or more point mutations, deletions, or inversions, but still encodes a functional MP protein. In still another embodiment, one or more of the regulatory regions a promoter, repressor, or inducer) of an MP gene in a microorganism has been altered by deletion, truncation, inversion, or point mutation) such that the expression of the MP gene is modulated. One of ordinary skill in the art will appreciate that host cells containing more than one of the described MP gene and protein modifications may be readily produced using the methods of the invention, and are meant to be included in the present invention.

A host cell of the invention, such as a prokaryotic or eukaryotic host cell in culture, can be used to produce express) an MP protein. Accordingly, the invention further provides methods for producing MP proteins using the host cells of the invention. In one embodiment, the method comprises culturing the host cell of invention (into which a recombinant expression vector encoding an MP protein has been introduced, or into which genome has been introduced a gene encoding a wild-type or altered MP protein) in a suitable medium until MP protein is produced. In another embodiment, the method further comprises isolating MP proteins from the medium or the host cell.

C. Isolated MP Proteins Another aspect of the invention pertains to isolated MP proteins, and biologically active portions thereof. An "isolated" or "purified" protein or biologically active portion thereof issubstantially free of cellular material when produced by recombinant DNA techniques, or chemical precursors or other chemicals when chemically synthesized.

The language "substantially free of cellular material" includes preparations of MP protein in which the protein is separated from cellular components of the cells in which WO 01/00843 PCT/IB00/00923 -44it is naturally or recombinantly produced. In one embodiment, the language "substantially free of cellular material" includes preparations of MP protein having less than about 30% (by dry weight) of non-MP protein (also referred to herein as a "contaminating protein"), more preferably less than about 20% of non-MP protein, still more preferably less than about 10% of non-MP protein, and most preferably less than about 5% non-MP protein. When the MP protein or biologically active portion thereof is recombinantly produced, it is also preferably substantially free of culture medium, i.e., culture medium represents less than about 20%, more preferably less than about and most preferably less than about 5% of the volume of the protein preparation. The language "substantially free of chemical precursors or other chemicals" includes preparations of MP protein in which the protein is separated from chemical precursors or other chemicals which are involved in the synthesis of the protein. In one embodiment, the language "substantially free of chemical precursors or other chemicals" includes preparations of MP protein having less than about 30% (by dry weight) of chemical precursors or non-MP chemicals, more preferably less than about 20% chemical precursors or non-MP chemicals, still more preferably less than about 10% chemical precursors or non-MP chemicals, and most preferably less than about 5% chemical precursors or non-MP chemicals. In preferred embodiments, isolated proteins or biologically active portions thereof lack contaminating proteins from the same organism from which the MP protein is derived. Typically, such proteins are produced by recombinant expression of, for example, a C. glutamicum MP protein in a microorganism such as C. glutamicum.

An isolated MP protein or a portion thereof of the invention can catalyze an enzymatic reaction in an amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathway, or has one or more of the activities set forth in Table 1. In preferred embodiments, the protein or portion thereof comprises an amino acid sequence which is sufficiently homologous to an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) such that the protein or portion thereof maintains the ability to catalyze an enzymatic reaction in an amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathway. The portion of the protein is preferably a biologically active portion as described herein. In another preferred embodiment, an MP protein of WO 01/00843 PCT/IB00/00923 the invention has an amino acid sequence set forth as an even-numbered SEQ ID NO: of the Sequence Listing. In yet another preferred embodiment, the MP protein has an amino acid sequence which is encoded by a nucleotide sequence which hybridizes, e.g., hybridizes under stringent conditions, to a nucleotide sequence of the invention a sequence of an odd-numbered SEQ ID NO: of the Sequence Listing). In still another preferred embodiment, the MP protein has an amino acid sequence which is encoded by a nucleotide sequence that is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 76%, 77%, 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 85%, 86%, 87%, 88%, 89%, or or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to one of the nucleic acid sequences of the invention, or a portion thereof. Ranges and identity values intermediate to the above-recited values, 70-90% identical or 80-95% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included. The preferred MP proteins of the present invention also preferably possess at least one of the MP activities described herein. For example, a preferred MP protein of the present invention includes an amino acid sequence encoded by a nucleotide sequence which hybridizes, hybridizes under stringent conditions, to a nucleotide sequence of the invention, and which can catalyze an enzymatic reaction in an amino acid, vitamin, cofactor, nutraceutical, nucleotide, nucleoside, or trehalose metabolic pathway, or which has one or more of the activities set forth in Table 1.

In other embodiments, the MP protein is substantially homologous to an amino acid sequence of the invention a sequence of an even-numbered SEQ ID NO: of the Sequence Listing) and retains the functional activity of the protein of one of the amino acid sequences of the invention yet differs in amino acid sequence due to natural variation or mutagenesis, as described in detail in subsection I above. Accordingly, in another embodiment, the MP protein is a protein which comprises an amino acid sequence which is at least about 50%, 51%, 52%, 53%, 54%, 55%, 56%, 57%, 58%, 59%, or 60%, preferably at least about 61%, 62%, 63%, 64%, 65%, 66%, 67%, 68%, 69%/a, or 70%, more preferably at least about 71%, 72%, 73%, 74%, 75%, 76%, 77%, WO 01/00843 PCTIB00/00923 -46- 78%, 79%, or 80%, 81%, 82%, 83%, 84%, 87%, 88%, 89%, or 90%, or 91%, 92%, 93%, 94%, and even more preferably at least about 95%, 96%, 97%, 98%, 99% or more homologous to an entire amino acid sequence of the invention and which has at least one of the MP activities described herein. Ranges and identity values intermediate to the above-recited values, 70-90% identical or 80-95% identical) are also intended to be encompassed by the present invention. For example, ranges of identity values using a combination of any of the above values recited as upper and/or lower limits are intended to be included. In another embodiment, the invention pertains to a full length C. glutamicum protein which is substantially homologous to an entire amino acid sequence of the invention.

Biologically active portions of an MP protein include peptides comprising amino acid sequences derived from the amino acid sequence of an MP protein, an amino acid sequence of an even-numbered SEQ ID NO: of the Sequence Listing or the amino acid sequence of a protein homologous to an MP protein, which include fewer amino acids than a full length MP protein or the full length protein which is homologous to an MP protein, and exhibit at least one activity of an MP protein. Typically, biologically active portions (peptides, peptides which are, for example, 5, 10, 15, 20, 30, 35, 36, 37, 38, 39, 40, 50, 100 or more amino acids in length) comprise a domain or motif with at least one activity of an MP protein. Moreover, other biologically active portions, in which other regions of the protein are deleted, can be prepared by recombinant techniques and evaluated for one or more of the activities described herein. Preferably, the biologically active portions of an MP protein include one or more selected domains/motifs or portions thereof having biological activity.

MP proteins are preferably produced by recombinant DNA techniques. For example, a nucleic acid molecule encoding the protein is cloned into an expression vector (as described above), the expression vector is introduced into a host cell (as described above) and the MP protein is expressed in the host cell. The MP protein can then be isolated from the cells by an appropriate purification scheme using standard protein purification techniques. Alternative to recombinant expression, an MP protein, polypeptide, or peptide can be synthesized chemically using standard peptide synthesis techniques. Moreover, native MP protein can be isolated from cells endothelial WO 01/00843 PCTfIB00/00923 -47cells), for example using an anti-MP antibody, which can be produced by standard techniques utilizing an MP protein or fragment thereof of this invention.

The invention also provides MP chimeric or fusion proteins. As used herein, an MP "chimeric protein" or "fusion protein" comprises an MP polypeptide operatively linked to a non-MP polypeptide. An "MP polypeptide" refers to a polypeptide having an amino acid sequence corresponding to MP, whereas a "non-MP polypeptide" refers to a polypeptide having an amino acid sequence corresponding to a protein which is not substantially homologous to the MP protein, a protein which is different from the MP protein and which is derived from the same or a different organism. Within the fusion protein, the term "operatively linked" is intended to indicate that the MP polypeptide and the non-MP polypeptide are fused in-frame to each other. The non-MP polypeptide can be fused to the N-terminus or C-terminus of the MP polypeptide. For example, in one embodiment the fusion protein is a GST-MP fusion protein in which the MP sequences are fused to the C-terminus of the GST sequences. Such fusion proteins can facilitate the purification of recombinant MP proteins. In another embodiment, the fusion protein is an MP protein containing a heterologous signal sequence at its Nterminus. In certain host cells mammalian host cells), expression and/or secretion of an MP protein can be increased through use of a heterologous signal sequence.

Preferably, an MP chimeric or fusion protein of the invention is produced by standard recombinant DNA techniques. For example, DNA fragments coding for the different polypeptide sequences are ligated together in-frame in accordance with conventional techniques, for example by employing blunt-ended or stagger-ended termini for ligation, restriction enzyme digestion to provide for appropriate termini, filling-in of cohesive ends as appropriate, alkaline phosphatase treatment to avoid undesirable joining, and enzymatic ligation. in another embodiment, the fusion gene can be synthesized by conventional techniques including automated DNA synthesizers.

Alternatively, PCR amplification of gene fragments can be carried out using anchor primers which give rise to complementary overhangs between two consecutive gene fragments which can subsequently be annealed and reamplified to generate a chimeric gene sequence (see, for example, Current Protocols in Molecular Biology, eds. Ausubel et al. John Wiley Sons: 1992). Moreover, many expression vectors are commercially available that already encode a fusion moiety a GST polypeptide). An MP- WO 01/00843 PCT/IB00/00923 -48encoding nucleic acid can be cloned into such an expression vector such that the fusion moiety is linked in-frame to the MP protein.

Homologues of the MP protein can be generated by mutagenesis, discrete point mutation or truncation of the MP protein. As used herein, the term "homologue" refers to a variant form of the MP protein which acts as an agonist or antagonist of the activity of the MP protein. An agonist of the MP protein can retain substantially the same, or a subset, of the biological activities of the MP protein. An antagonist of the MP protein can inhibit one or more of the activities of the naturally occurring form of the MP protein, by, for example, competitively binding to a downstream or upstream member of the MP cascade which includes the MP protein. Thus, the C. glutamicum MP protein and homologues thereof of the present invention may modulate the activity of one or more metabolic pathways in which MP proteins play a role in this microorganism.

In an alternative embodiment, homologues of the MP protein can be identified by screening combinatorial libraries of mutants, truncation mutants, of the MP protein for MP protein agonist or antagonist activity. In one embodiment, a variegated library of MP variants is generated by combinatorial mutagenesis at the nucleic acid level and is encoded by a variegated gene library. A variegated library of MP variants can be produced by, for example, enzymatically ligating a mixture of synthetic oligonucleotides into gene sequences such that a degenerate set of potential MP sequences is expressible as individual polypeptides, or alternatively, as a set of larger fusion proteins for phage display) containing the set of MP sequences therein.

There are a variety of methods which can be used to produce libraries of potential MP homologues from a degenerate oligonucleotide sequence. Chemical synthesis of a degenerate gene sequence can be performed in an automatic DNA synthesizer, and the synthetic gene then ligated into an appropriate expression vector. Use of a degenerate set of genes allows for the provision, in one mixture, of all of the sequences encoding the desired set of potential MP sequences. Methods for synthesizing degenerate oligonucleotides are known in the art (see, Narang, S.A. (1983) Tetrahedron 39:3; Itakura et al. (1984) Annu. Rev. Biochem. 53:323; Itakura et al. (1984) Science 198:1056; Ike et al. (1983) Nucleic Acid Res. 11:477.

WO 01/00843 PCTIIBOO/00923 -49- In addition, libraries of fragments of the MP protein coding can be used to generate a variegated population of MP fragments for screening and subsequent selection of homologues of an MP protein. In one embodiment, a library of coding sequence fragments can be generated by treating a double stranded PCR fragment of an MP coding sequence with a nuclease under conditions wherein nicking occurs only about once per molecule, denaturing the double stranded DNA, renaturing the DNA to form double stranded DNA which can include sense/antisense pairs from different nicked products, removing single stranded portions from reformed duplexes by treatment with S nuclease, and ligating the resulting fragment library into an expression vector. By this method, an expression library can be derived which encodes N-terminal, C-terminal and internal fragments of various sizes of the MP protein.

Several techniques are known in the art for screening gene products of combinatorial libraries made by point mutations or truncation, and for screening cDNA libraries for gene products having a selected property. Such techniques are adaptable for rapid screening of the gene libraries generated by the combinatorial mutagenesis of MP homologues. The most widely used techniques, which are amenable to high through-put analysis, for screening large gene libraries typically include cloning the gene library into replicable expression vectors, transforming appropriate cells with the resulting library of vectors, and expressing the combinatorial genes under conditions in which detection of a desired activity facilitates isolation of the vector encoding the gene whose product was detected. Recursive ensemble mutagenesis (REM), a new technique which enhances the frequency of functional mutants in the libraries, can be used in combination with the screening assays to identify MP homologues (Arkin and Yourvan (1992) PNAS 89:7811-7815; Delgrave et al. (1993) Protein Engineering 6(3):327-331).

In another embodiment, cell based assays can be exploited to analyze a variegated MP library, using methods well known in the art.

D. Uses and Methods of the Invention The nucleic acid molecules, proteins, protein homologues, fusion proteins,.

primers, vectors, and host cells described herein can be used in one or more of the following methods: identification of C. glutamicum and related organisms; mapping of genomes of organisms related to C. glutamicum; identification and localization of C.

WO 01/00843 PCT/IB00/00923 glutamicum sequences of interest; evolutionary studies; determination of MP protein regions required for function; modulation of an MP protein activity; modulation of the activity of an MP pathway; and modulation of cellular production of a desired compound, such as a fine chemical.

The MP nucleic acid molecules of the invention have a variety of uses. First, they may be used to identify an organism as being Corynebacterium glutamicum or a close relative thereof. Also, they may be used to identify the presence of C. glutamicum or a relative thereof in a mixed population of microorganisms. The invention provides the nucleic acid sequences of a number of C. glutamicum genes; by probing the extracted genomic DNA of a culture of a unique or mixed population of microorganisms under stringent conditions with a probe spanning a region of a C. glutamicum gene which is unique to this organism, one can ascertain whether this organism is present.

Although Corynebacterium glutamicum itself is not pathogenic to humans, it is related to species which are human pathogens, such as Corynebacterium diphtheriae.

Corynebacterium diphtheriae is the causative agent of diphtheria, a rapidly developing, acute, febrile infection which involves both local and systemic pathology. In this disease, a local lesion develops in the upper respiratory tract and involves necrotic injury to epithelial cells; the bacilli secrete toxin which is disseminated through this lesion to distal susceptible tissues of the body. Degenerative changes brought about by the inhibition of protein synthesis in these tissues, which include heart, muscle, peripheral nerves, adrenals, kidneys, liver and spleen, result in the systemic pathology of the disease. Diphtheria continues to have high incidence in many parts of the world, including Africa, Asia, Eastern Europe and the independent states of the former Soviet Union. An ongoing epidemic of diphtheria in the latter two regions has resulted in at least 5,000 deaths since 1990.

In one embodiment, the invention provides a method of identifying the presence or activity of Cornyebacterium diphtheriae in a subject. This method includes detection of one or more of the nucleic acid or amino acid sequences of the invention the sequences set forth as odd-numbered or even-numbered SEQ ID NOs, respectively, in the Sequence Listing) in a subject, thereby detecting the presence or activity of Corynebacterium diphtheriae in the subject. C. glutamicum and C. diphtheriae are related bacteria, and many of the nucleic acid and protein molecules in C. glutamicum WO 01/00843 PCT/IB00/00923 -51 are homologous to C. diphtheriae nucleic acid and protein molecules, and can therefore be used to detect C. diphtheriae in a subject.

The nucleic acid and protein molecules of the invention may also serve as markers for specific regions of the genome. This has utility not only in the mapping of the genome, but also for functional studies of C. glutamicum proteins. For example, to identify the region of the genome to which a particular C. glutamicum DNA-binding protein binds, the C. glutamicum genome could be digested, and the fragments incubated with the DNA-binding protein. Those which bind the protein may be additionally probed with the nucleic acid molecules of the invention, preferably with readily detectable labels; binding of such a nucleic acid molecule to the genome fragment enables the localization of the fragment to the genome map of C. glutamicum, and, when performed multiple times with different enzymes, facilitates a rapid determination of the nucleic acid sequence to which the protein binds. Further, the nucleic acid molecules of the invention may be sufficiently homologous to the sequences of related species such that these nucleic acid molecules may serve as markers for the construction of a genomic map in related bacteria, such as Brevibacterium lactofermentum.

The MP nucleic acid molecules of the invention are also useful for evolutionary and protein structural studies. The metabolic processes in which the molecules of the invention participate are utilized by a wide variety of prokaryotic and eukaryotic cells; by comparing the sequences of the nucleic acid molecules of the present invention to those encoding similar enzymes from other organisms, the evolutionary relatedness of the organisms can be assessed. Similarly, such a comparison permits an assessment of which regions of the sequence are conserved and which are not, which may aid in determining those regions of the protein which are essential for the functioning of the enzyme. This type of determination is of value for protein engineering studies and may give an indication of what the protein can tolerate in terms of mutagenesis without losing function.

Manipulation of the MP nucleic acid molecules of the invention may result in the production of MP proteins having functional differences from the wild-type MP proteins. These proteins may be improved in efficiency or activity, may be present in greater numbers in the cell than is usual, or may be decreased in efficiency or activity.

WO 01/00843 PCTfIB00/00923 -52- The invention also provides methods for screening molecules which modulate the activity of an MP protein, either by interacting with the protein itself or a substrate or binding partner of the MP protein, or by modulating the transcription or translation of an MP nucleic acid molecule of the invention. In such methods, a microorganism expressing one or more MP proteins of the invention is contacted with one or more test compounds, and the effect of each test compound on the activity or level of expression of the MP protein is assessed.

When the desired fine chemical to be isolated from large-scale fermentative culture of C. glutamicum is an amino acid, a vitamin, a cofactor, a nutraceutical, a nucleotide, a nucleoside, or trehalose, modulation of the activity or efficiency of activity of one or more of the proteins of the invention by recombinant genetic mechanisms may directly impact the production of one of these fine chemicals. For example, in the case of an enzyme in a biosynthetic pathway for a desired amino acid, improvement in efficiency or activity of the enzyme (including the presence of multiple copies of the gene) should lead to an increased production or efficiency of production of that desired amino acid. In the case of an enzyme in a biosynthetic pathway for an amino acid whose synthesis is in competition with the synthesis of a desired amino acid, any decrease in the efficiency or activity of this enzyme (including deletion of the gene) should result in an increase in production or efficiency of production of the desired amino acid, due to decreased competition for intermediate compounds and/or energy. In the case of an enzyme in a degradation pathway for a desired amino acid, any decrease in efficiency or activity of the enzyme should result in a greater yield or efficiency of production of the desired product due to a decrease in its degradation. Lastly, mutagenesis of an enzyme involved in the biosynthesis of a desired amino acid such that this enzyme is no longer is capable of feedback inhibition should result in increased yields or efficiency of production of the desired amino acid. The same should apply to the biosynthetic and degradative enzymes of the invention involved in the metabolism of vitamins, cofactors, nutraceuticals, nucleotides, nucleosides and trehalose.

Similarly, when the desired fine chemical is not one of the aforementioned compounds, the modulation of activity of one of the proteins of the invention may still impact the yield and/or efficiency of production of the compound from large-scale culture of C. glutamicum. The metabolic pathways of any organism are closely WO 01/00843 PCT/IB00/00923 -53interconnected; the intermediate used by one pathway is often supplied by a different pathway. Enzyme expression and function may be regulated based on the cellular levels of a compound from a different metabolic process, and the cellular levels of molecules necessary for basic growth, such as amino acids and nucleotides, may critically affect the viability of the microorganism in large-scale culture. Thus, modulation of an amino acid biosynthesis enzyme, for example, such that it is no longer responsive to feedback inhibition or such that it is improved in efficiency or turnover may result in increased cellular levels of one or more amino acids. In turn, this increased pool of amino acids provides not only an increased supply of molecules necessary for protein synthesis, but also of molecules which are utilized as intermediates and precursors in a number of other biosynthetic pathways. Ifa particular amino acid had been limiting in the cell, its increased production might increase the ability of the cell to perform numerous other metabolic reactions, as well as enabling the cell to more efficiently produce proteins of all kinds, possibly increasing the overall growth rate or survival ability of the cell in large scale culture. Increased viability improves the number of cells capable of producing the desired fine chemical in fermentative culture, thereby increasing the yield of this compound. Similar processes are possible by the modulation of activity of a degradative enzyme of the invention such that the enzyme no longer catalyzes, or catalyzes less efficiently, the degradation of a cellular compound which is important for the biosynthesis of a desired compound, or which will enable the cell to grow and reproduce more efficiently in large-scale culture. It should be emphasized that optimizing the degradative activity or decreasing the biosynthetic activity of certain molecules of the invention may also have a beneficial effect on the production of certain fine chemicals from C. glutamicum. For example, by decreasing the efficiency of activity of a biosynthetic enzyme in a pathway which competes with the biosynthetic pathway of a desired compound for one or more intermediates, more of those intermediates should be available for conversion to the desired product. A similar situation may call for the improvement of degradative ability or efficiency of one or more proteins of the invention.

This aforementioned list of mutagenesis strategies for MP proteins to result in increased yields of a desired compound is not meant to be limiting; variations on these mutagenesis strategies will be readily apparent to one of ordinary skill in the art. By WO 01/00843 PCT/IB00/00923 -54these mechanisms, the nucleic acid and protein molecules of the invention may be utilized to generate C. glutamicum or related strains of bacteria expressing mutated MP nucleic acid and protein molecules such that the yield, production, and/or efficiency of production of a desired compound is improved. This desired compound may be any natural product of C. glutamicum, which includes the final products of biosynthesis pathways and intermediates of naturally-occurring metabolic pathways, as well as molecules which do not naturally occur in the metabolism of C. glutamicum, but which are produced by a C. glutamicum strain of the invention.

This invention is further illustrated by the following examples which should not be construed as limiting. The contents of all references, patent applications, patents, published patent applications, Tables, and the sequence listing cited throughout this application are hereby incorporated by reference.

TABLE 1: Included Genes Lysine biosynthesis Nucleic Acid SEQ ID NO 3 7 9 11 Amino Acid SEQ ID NO 2 4 6 8 10 12 Identification Code RXA02229 RXS02970 F RXAOI 09 RXC02390 RXCO1 798 RXCO1 207 Contig. NT Start GR00653 2793 GR00287 4714 NT Stop 3617 5943 Function DIAMINOPIMELATE EPIMERASE (EC 5.1.1.7) ACETYLORNITHINE AMINOTRANSFERASE (EC 2.6.1.11) ACIETYLORNITHINE AMINOTRANSFERASE (EC 2.6. 1.11) MEMBRANE SPANNING PROTEIN INVOLVED IN LYSINE METABOLISM MEMBRANE ASSOCIATED PROTEIN INVOLVED IN LYSINE METABOLISM CYTOSOLIC PROTEIN INVOLVED IN METABOLISM OF LYSINE AND

THREONINE

TRANSCRIPTIONAL REGULATOR INVOLVED IN LYSINE METABOLISM CYTOSOLIC PROTEIN INVOLVED IN LYSINE METABOLISM RXCO0657 RXCO0552 Trehalose Nuclelc Acid Amino Acid SEQ ID NO SEQ ID NO 17 18 19 20 Identification Code RXN00351 F RXA00351 RXA00873 RXA00891 Contig. NT Start W0135 37078 GR00066 1486 GR00241 3 GR00243 1005 NT Stop Function 38532 ALPHA,ALPIIA-TREI-ALOSE-PH-OSPHiATE SYNTHASE (UDP-FORMING) 56 KO SUBUNIT (EC 2.4.1.15) 2931 ALPHAALPIIA-TREH-ALOSE-PKOSPHATE SYNTHASE (UDP-FORMING) 56 KD SUBUNIT (EC 2.4.1.15) 758 trehalose synthase (EC 4 trehalose synthase (EC Lysine biosynthesis Nucleic Acid SEQ ID NO 27 29 31 .33 37 39 Amino Acid SEQ ID NO 26 28 30 32 34 36 38 40 Identification Code RXA00534 RXA00533 RXA02843 RXA02022 RXA00044 RXA00863 RXA00864 RXA02843 RXN00355 F RXA00352 Contig. NT Start NT Stop Function GROO137 4758 3496 ASPARTOKINASE ALPHA AND BETA SUBUNITS (EC 2.7.2.4) GR00137 3469 2438 ASPARTATE-SEMIALDEHYDE DEHYDROGENASE (EC 1.2.1.11) GR00842 543 4 2,3,4,5-TETRAHYDROPYRIDINE-2-CARBOXYLATE N-SUCCINYLTRANSFERASE (EC 2.3.1.117) GROO613 2083 3169 SUCCINYL-DIAMINOPIMELATE DESUCCINYLASE (EC 3.5.1.18) GROO007 3458 4393 DI1HYDRODIPICOLINATE SYNTHASE (EC 4.2.1.52) GR00236 896 1639 DIHYDRODIPICOLINATE REDUCTASE (EC 1.3.1.26) GR00236 1694 2443 probable 2,3-dihydrodipicolinate N-C6-Iyase (cyclizing) (EC Corynebacterium glutamicum GR00842 543 4 2,3,4,5-TETRAHYDROPYRIDINE-2-CARBOXYLATE N-SUCCINYLTRANSFERASE (EC 2.3.1.117) W01 35 31980 30961 MESO-DIAMINOPIMELATE D-DEHYDROGENASE GR00068 881 4 MESO-DIAMINOPIMELATE D-DEHYDROGENASE (EC 1.4.1.16) Nucleic Acid SEQ ID NO 47 49 51 53 57 Amino Acid SEQ ID NO 46 48 50 52 54 56 58 Identification Code Contig.

RXA00972 RXA02653 RXA01 393 RXAO0241 RXA01 394 RXA00865 RXS02021 RXS021 57 RXC00733 RXCO0861 RXC00866 RXC02095 RXCO31 85 GR00274 GR00752 GROD408 GR00036 GR00408 GR00236 Table I (continued) NT Start NT Stop Function 3 1379 DIAMINOPIMELATE DECARBOXYLASE (EC 4.1.1.20) 5237 7234 DIAMINOPIMELATE DECARBOXYLASE (EC 4.1.1.20) 4249 3380 LYSINE EXPORT REGULATOR PROTEIN 5443 6945 L-LYSINE TRANSPORT PROTEIN 4320 5018 LYSINE EXPORTER PROTEIN 2647 3549 DIHYDRODIPICOLINATE SYNTHASE (EC 4.2.1.52) 2,3.4,5-TETRAHYDROPYRIDINE-2-CARBOXYLATE N-SUCCINYLTRANSFERASE (EC 2.3.1.117) ACETYLORNIT-INE AMINOTRANSFERASE (EC 2.6.1.11) ABC TRANSPORTER ATP-BINDING PROTEIN INVOLVED IN LYSINE

METABOLISM

PROTEIN INVOLVED IN LYSINE METABOLISM ZN-DEPENDENT HYDROLASE INVOLVED IN LYSINE METABOLISM ABC TRANSPORTER ATP-BINDING PROTEIN INVOLVED IN LYSINE

METABOLISM

PROTEIN INVOLVED IN LYSINE METABOLISM Glutamnate and glutamnine metabolism Nucleic Acid SEQ ID NO 71 73 77 79 81 83 87 89 91 93 97 99 101 103 105 107 Amino Acid Identification Code Contig.

SEQ ID NO 72 RXN00367 W0196 74 F RXA00007 GRO0001 76 F RXA00364 GRO0074 78 F RXA00367 GR00075 80 RXN00076 W0154 82 F RXA00075 GROO012 84 RXNOOI198 W0181 86 F RXA00198 GR00031 88 RXN00365 W0196 90 F RXA00365 GR00075 92 RXA00366 GR00075 94 RXA02072 GR00628 96 RXA00323 GR00057 98 RXA00335 GR00057 100 RXA00324 GR00057 102 RXN03176 W0332 104 F RXA02879 GR10017 106 RXA00278 GR00043 108 RXA00727 GR00193 NT Start NT Stop Function 9744 7107 1296 1806 2752 2757 7916 2 14.607 630 961 1259 3855 19180 5262 2 2 2612 614 14273 8912 4 964 4122 3419 7368 283 15233 4 605 2599 5192 17750 8396 862 862 1581 1525 GLUTAMATE SYNTHASE [NADH] PRECURSOR (EC 1.4.1.14) GLUTAMATE SYNTHASE (NADPH) LARGE CHAIN PRECURSOR (EC 1.4.1.13) GLUTAMATE SYNTHASE (NADPH) LARGE CHAIN PRECURSOR (EC 1.4.1.13) GLUTAMATE SYNTHASE (NAOPH) LARGE CHAIN PRECURSOR (EC 1.4.1.13) GLUTAMATE SYNTHASE (NADPH) SMALL CHAIN (EC 1.4.1.13) GLUTAMATE SYNTHASE (NADP-) SMALL CHAIN (EC 1.4.1.13) GLUTAMATE SYNTHASE (NADPH) SMALL CHAIN (EC 1.4.1.13) GLUTAMATE SYNTHASE (NADPH) SMALL CHAIN (EC 1.4.1.13) GLUTAMATE SYNTHASE (NADPHJ SMALL. CHAIN (EC 1.4.1.13) GLUTAMATE SYNTHASE (NADPH) SMALL CHAIN (EC 1.4.1.1 3) GLUTAMATE SYNTHASE (NADPH) SMALL CHAIN (EC 1.4.1.13) NADP-SPECIFIC GLUTAMATE DEHYDROGENASE (EC 1.4.1.4) GLUTAMINE SYNTHETASE (EC 6.3.1.2) GLUTAMINE'SYNTHETASE (EC 6.3.1.2) GLUTAMATE-AMMONIA-LIGASE ADENYLYLTRANSFERASE (EC 2.7.7.42) GLUTAMINASE (EC 3.5.1.2) GLUTAMINASE (EC 3.5.1.2) GLUTAMINE-BINDING PROTEIN PRECURSOR GLUTAMINE-BINDING PERIPLASMIC PROTEIN PRECURSOR Table I (continued) Alanine and Aspartate and Asparagine metabolism Nudeic Acid SEQ ID NO 109 ill 113 115 117 119 121 123 125 127 129 131 Amino Acid Identification Code SEQ ID NO 110 RXA02139 112 RXN00116 114 F RXAOO1 16 116 RXN00618 118 F RXAOD618 120 F RXA0O627 122 RXA02550 124 RXA02193 126 RXA02432 128 RXN03003 130 RXN00508 132 RXN00636 GR00639 GR00018 W01 35 GROO163 GROO1 64 GR00729 GR00645 GR00708 W01 38 W0086 W0135 NT Start 6739 26974 510 10288 213 854 1585 1942 2669 680 4701 20972 NT Stop 4901 25814 4 9182 746 1138 275 365 1695 6 5783 19944 Function ASPARAGINE SYNTHETASE (GLUTAMINE-HYDROLYZING) (EC 6.3.5.4) ASPARTATE AMINOTRANSFERASE (EC 2.6.1.1) ASPARTATE AMINOTRANSFERASE (EC 2.6.1.1) ASPARTATE AMINOTRANSFERASE (EC 2.6.1.1) ASPARTATE AMINOTRANSFERASE (EC 2.6.1.1) ASPARTATE AMINOTRANSFERASE (EC 2.6.1. 1) ASPARTATE AMINOTRANSFERASE (EC 2.6.1.1) ASPARTATE ANIMONIA-LYASE (EC 4.3.1.1) L-ASPARAGINASE (EC 3.5.1.1) ASPARTATE AM INOTRANSFERASE (EC 2.6.1. 1) ALANINE RACEMASE (EC 5.1.1.1) ALANINE RACEMASE, BIOSYNTHETIC (EC 5.1.1.1) beta-Alanine metabolism Nudeic Acid SEQ ID NO 133 135 137 Amino Acid Identification Code SEQ ID NO 134 RXA02536 136 RXS00870 138 RXS02299 Contig.

NT Start NT Stop Functin GR00726 8581 7826 BETA-UREIDOPROPIONASE (EC 3.5.1.6) METHYLMALONATE-SEMIALDEHYDE DEHYDROGENASE (ACYLATING) (EC 1.2.1.27) ASPARTATE 1-DECARBOXYLASE PRECURSOR (EC 4.1.1.11) Glycine and senine metabolism Nucleic Acid Aino Acid Identification Code SEQ ID NO SEQ ID NO 139 140 RXA01561 141 142 RXA01 850 143 144 RXA00580 145 146 RXA01821 147 148 RXN02263 149 150 F RXA02263 151 152 RXA02176 153 154 RXN02758 155 156 F RXA02479 157 158 F RXA02758 159 160 F RXA02759 161 162 RXA02501 160 164 RXN031 05 165 166 RXSO1 130 167 168 RXS03112 9oRt!g.

GR00435 GR00525 GROO1 56 GR0051 5 W0202 GR0D654 GROO641 GR00766 GR00717 GR00766 GR00766 GR00720 W0074 NT Start NT Stop Function 1113 481 7343 10253 11783 33454 11454 5082 393 5082 5330 15041 15857 2042 1827 6042 9876 12160 33813 12581 4648 4 4648 5220 13977 15423 L-SERINE DEHYDRATASE (EC 4.2.1.13) L-SERINE DEHYDRATASE (EC 4.2.1.13) SERINE HYDROXYMETHYLTRANSFERASE (EC 2.1.2.1) SARCOSINE OXIDASE (EC 1.5.3.1) SARCOSINE OXIDASE (EC 1.5.3.1) SARCOSINE OXIDASE (EC 1.5.3.1) PHOSPHOSERINE AMINOTRANSFERASE (EC 2.6.1.52) PHOSPHOSERINE PHOSPHATASE (EC 3.1.3.3) PHOSPHOSERINE PHOSPHATASE (EC 3.1.3.3) PHOSPHOSERINE PHOSPHATASE (EC 3.1.3.3) PHOSPHOSERINE PHOSPHATASE (EC 3.1.3.3) PHOSPHOSERINE PHOSPHATASE (EC 3.1.3.3) SARCOSINE OXIDASE (EC 1.5.3.1) D-3-PHOSPHOGLYCERATE DEHYDROGENASE (EC 1.1.1.95) 0-3-PHOSPHOGLYCERATE DEHYDROGENASE (EC 1.1.1.95) Table 1 (continued) Threonine metabolism Nucleic Acid SEQ ID NO 169 171 173 175 177 179 181 183 Amino Acid SEQ ID NO 170 172 174 176 178 180 182 184 Identification Code RXN00969 F RXA00974 RXA00970 RXA00330 RXNOD403 F RXA00403 RXCO1 207 RXCO0152 Contig.

WV0149 GR00274 GR00273 GROO057 W0086 GR00088 NT Start 12053 2623 161 12968 70041 723 NT Stop 13387 3015 1087 14410 68911 1 832 Function HOMOSERINE DEHYDROGENASE (EC 1.1.1.3) HOMOSERINE DEHYDROGENASE (EC 1.1.1.3) HOMOSERINE KINASE (EC 2.7.1.39) THREONINE SYNTHASE (EC 4.2.99.2) HOMOSERINE 0-ACETYLTRANSFERASE HOMOSERINE 0-ACETYLTRANSFERASE (EC 2.3.1.11) CYTOSOLIC PROTEIN INVOLVED IN METABOLISM OF LYSINE AND

THREONINE

MEMBRANE ASSOCIATED PROTEIN INVOLVED IN THREONINE METABOLISM Metabolism of methionine and S-adenosyl methionine Nucleic Acid SEQ ID NO 185 187 189 191 193 195 197 199 201 203 205 207 209 211 213 215 217 219 221 Amino Acid SEQ ID NO 186 188 190 192 '194 196 198 200 202 204 206 208 210 212 214 216 218 220 222 identification Code RXA001 15 RXN00403 F RXAOD403 RXS031 58 F RXA00254 RXA02532 RXS03159 F RXA02768 RXA00216 RXN00402 F RXA00402 RXA00405 RXA021 97 RXN02 198 F RXA02198 RXN03074 F RXA02906 RXN00132 F RXA00132 GR00017 WV0086 GR00088 GR00038 GR00726 GR00770 GR00032 WV0086 GR00088 GR00089 GR00645 WV0302 GR00646 W0042 GRI 0044 WV0124 GR00020 NT Start 5359 70041 723 2404 3085 1919 16286 70787 3289 4552 9228 2483 2238 1142 3612 7728 NT Sto_ 4313 68911 1832 1811 2039 2521 15297 70188 576 3801 4025 11726 6 1741 645 5045 7624 Function HOMOSERINE 0-ACETYLTRANSFERASE (EC 2.3.1.31) HOMOSERINE 0-ACETYLTRANSFERASE HOMOSERINE 0-ACETYLTRANSFERASE (EC 2.3.1.11) CYSTATHIONINE GAMMA-SYNTHASE (EC 4.2.99.9) CYSTATHIONINE GAMMA-SYNTH-ASE (EC 4.2.99.9) CYSTATHIONINE GAMMA-SYNTHASE (EC 4.2.99.9) CYSTATHIONINE GAMMA-SYNTHASE (EC 4.2.99.9) CYSTATHIONINE GAMMA-SYNTHASE (EC 4.2.99.9) 5-mthyltetrahydrofolate-homocysteine methyftransferase (methionine synthetase) O-ACETYLHOMOSERINE SULFHYDRYLASE (EC 4.2.99. 10)1 /0-ACETYLSERINE SULFHYDRYLASE (EC 4.2.99.8) O-ACETYLHOMOSERINE SULFH-YDRYLASE (EC 4.2.99.10)1 /0-ACETYLSERINE SULFHYDRYLASE (EC 4.2.99.8) O-ACETYLHOMOSERINE SULFHYDRYLASE (EC 4.2.99.10)1 /0-ACETYLSERINE SULFHYDRYLASE (EC 4.2.99.8) 5-METHYLTETRAHYDROFOLATE-HOMOCYSTEINE METHYLTRANSFERASE (EC 2.1.1.13) 5-METHYLTETRAHYDROFOLATE-HOMOCYSTEINE METHYLTRANSFERASE (EC 2.1.1.13) 5-METHYLTETRAHYDROFOLATE-HOMOCYSTEINE METHYLTRANSFERASE (EC 2.1.1.13) S-ADENOSYLMETHIONINE:2-DEMETHYLMENAQUINONE METHYLTRANSFERASE (EC S-ADENOSYLMETHIONINE:2-DEMETHYLMENAQUINONE METHYLTRANSFERASE (EC ADENOSYIHOMOCYSTEINASE (EC 3.3.1.1) ADENOSYLNOMOCYSTEINASE (EC 3.3.1.1) Nucleic Acid SEQ ID NO 223 225 227 229 231 233 235 237 239 Amino Acid SEQ ID NO 224 226 228 230 232 234 236 238 240 Identification Code F RXA01 371 RXN02085 F RXA02085 F RXA02086 RXN02648 F RXA02648 F RXA02658 RXC02238 RXC001 28 Contig GR00398 GR00629 GR00629 GR00751 GR00752 Table I (continued) NT Start NT Stop Function 2339 3634 ADENOSYLHOMOCYSTEINASE (EC 3.3.1.1) METHYLTRANSFERASE (EC 2.1.1.14) 3496 5295 METHYLTRANSFERASE (EC 2.1.1.14) 5252 5731 METHYLTRANSFERASE (EC 2.1.1.14) METHYLTRANSFERASE (EC 2.1.1.14) 5254 4730 METHYLTRANSFERASE (EC 2.1.1.14) 14764 15447 METHYLTRANSFERASE (EC 2.1.1.14) PROTEIN INVOLVED IN METABOLISM OF S-ADENOSYLMETHIONINE, PURINES AND PANTOTHENATE EXPORTED PROTEIN INVOLVED IN METABOLISM OF PYRIDIMES AND

ADENOSYLHOMOCYSTEINE

S-adenosyl methionine (SAM) Biosynthesis Nucleic Acid Amino Acid SEQ ID NO7 SEQ I1D NO 241 24 2 Identification Code Contig RXA02240 GROO654 NT Start 7160 NT Sto- 8380 Function S-ADENOSYLMETHIONINE SYNTHETASE (EC 2.5.1.6) Cysteine metabolism Nudleic Acdd SEQ ID NO 243 245 247 249 251 253 255 Amino Acid SEQ ID NO 244 246 248 250 252 254 256 Identification Code RXA00780 RXA00779 RXN00402 F RXAOD402 RXS00405 RXCO01 64 RXC01 191 GR00206 GR00206 WOO86 GROO088 NT Start 1689 550 70787 NT Sto, 2234 1482 70188 576 Function SERINE ACETYLTRANSFERASE (EC 2.3.1.30) CYSTEINE SYNTHASE (EC 4.2.99.8) O-ACETYLHOMOSERINE SULFHYDRYLASE (EC 4.2.99.10)1 /0-ACETYLSERINE SULFHYDRYLASE (EC 4.2.99.8) 0-ACETYLHOMOSERINE SULFHYDRYLASE (EC 4.2.99.10) /0-ACETYLSERINE SULFHYDRYLASE (EC 4.2.99.8) O-ACETYLHOMOSERINE SULFHYDRYLASE (EC 4.2.99.10)1 /0-ACETYLSERINE SULFHYDRYLASE (EC 4.2.99.8) ABC TRANSPORTER ATP-BINDING PROTEIN INVOLVED IN CYSTEINE

METABOLISM

ABC TRANSPORTER ATP-BINDING PROTEIN INVOLVED IN CYSTEINE

METABOLISM

Table 1 (continued) Valine, leucine and isoleucine Nucleic Acid Amino Acid SEQ ID NO SEQ IDNO 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 Identification Code Contip. NT Start NT Stop Function RXA02646 RXAD0766 RXN01 690 F RXA01690 RXN01 026 F RXA01026 RXN01 127 F RXA01 132 RXN00536 F RXA00536 RXN02965 RXN01929 F RXA01929 RXN0 1420 RXSO1 145 F RXA01 145 GROO751 GR00204 WV0246 GR00473 WO0143 GR00294 W0157 GROO31 5 WV021 9 GR00137 W0143 WV01 27 GR00555 WV0122 GR00321 3856 5091 1296 1248 9171 4491 1349 6128 6128 7711 47590 2766 15584 1075 2588 THREONINE DEHYDRATASE BIOSYNTHETIC (EC 4.2.1.16) 4249 BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE (EC 2.6.1.42) 196 BRANCHED-CHAIN AMINO ACID AMINOTRANSFE RASE (EC 2.6.1.42) 196 BRANCHED-CHAIN AMINO ACID AMINOTRANSFERASE (EC 2.6.1.42) 7513 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (EC 4.2.1.33) 1602 3-ISOPROPYLMALATE DEHYDRATASE LARGE SUBUNIT (EC 4.2.1.33) 3472 3-ISOPROPYLMALATE DEHYDROGENASE (EC 1. 1. 1.85) 1651 3-ISOPROPYLMALATE DEHYDROGENASE (EC 1.1.1.85) 7498 2-ISOPROPYLMALATE SYNTHASE (EC 4.1.3.12) 7360 2-ISOPROPYLMALATE SYNTHASE (EC 4.1.3.1) 7121 3-ISOPROPYLMVALATE DEHYDRATASE SMALL SUBUNIT (EC 4.2.1.33) 48402 3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE (EC 2.1.2.11) DECARBOXYLASE (EC 4.1.1.44) 1960 3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE (EC 2.1.2.11) 14643 4"-MYCAROSYL ISO VALERYL-COA TRANSFERASE (EC KETOL-ACID REDUCTOISOMERASE (EC 1.1.1.86) 1530 KETOL-ACID REDUCTOISOMERASE (EC 1.1.1.86) Arginine and proline metabolism Enzymes of proline biosynthesis: Nucleic Acid SEQ ID NO 289 291 293 295 297 299 301 303 305 Amino Acid Identification Code Contig.

SEQ ID NO 290 RXA02375 GR00689 292 RXN02382 W0213 294 F RXA02378 GR00690 296 F RXA02382 GR00691 298 RXA02499 GR00720 300 RXS02157 302 RXS02262 304 RXS02970 306 F RXA01009 GR00287 NT Start NT Stop Function 1449 5162 624 2493 11883 4714 223 GLUTAMATE 5-KINASE (EC 2.7.2.11) 3867 GAMMA-GLUTAMYL PHOSPHATE REDUCTASE (GPR) (EC 1.2.1.41) 16 GAMMA-GLUTAMYL PHOSPHATE REDUCTASE (GPR) (EC 1.2.1.41) 1894 GAMMA-GLUTAMYL PHOSPHATE REDUCTASE (GPR) (EC 1.2.1.41) 12692 PYRROLINE-5-CARBOXYLATE REDUCTASE (EC 1.5.1.2) ACETYLORNITHINE AMINOTRANSFERASE (EC 2.6. 1.11) ORNITHINE CYCLODEAMINASE (EC 4.3.1.12) ACETYLORNITHINE AMINOTRANSFERASE (EC 2.6. 1.11) 5943 ACETYLORNITHINE AMINOTRANSFERASE (EC 2.6.1.11) Enzymes of protine degradation: Nucleic Acid Amnino Acid Identification Code Conig.

SEQ ID NO SEQ ID NO 307 308 RXN00023 W0127 Table I (continued) 0 NT Start NT Stop Function 68158 64703 PROUNE DEHYDROGENASE (EC 1.5.99.8) DELTA-i PYRROLINE-5- 0 CARBOXYLATE DEHYDROGENASE (EC 1.5.1.12) 2 454 PROLINE DEHYDROGENASE (EC 1.5.99.8)1/DELTA-i- CARBOXYLATE DEHYDROGENASE (EC 1.5.1.12) 3028 5 PROLINE DEHYDROGENASE (EC 1.5.99.8)1/ DELTA-i CARBOXYLATE DEHYDROGENASE (EC 1.5.1.12) PROTEIN INVOLVED IN PROLINE METABOLISM 309 310 311 312 313 314 F RXA00023 F RXA02284 RXCO2498 GROO003 GROO660 Synthesis of 3-Hydoxy-proline: Nucleic Acid Amino Acid Identification Code Contig.

SEQ ID NO SEQ IDNO 315 316 RXA01491 GROO42 NT Start NT Stop Function ~3 5337 4687 DNA FOR L-PROLINE 3-HYDROXYLASE, COMPLETE COS Enzymes of omnithine, arginine and spermidine metabolism: Nudleic Acid SEQ ID NO 317 Amino Acid SEQ ID NO 318 Identification Code RXA021 55 RXA021 56 RXN02153 F RXA02153 RXA021 54 RXA021 57 RXS02970 F RXA01009 RXA021 58 RXA021 60 RXN02162 F RXA02161 F RXA02162 RXA02262 RXA0021 9 RXA01508 RXA0 1757 RXA021 59 RXN02154 RXS00147 RXS00905 RXS00906 qRntig.

NT Start NT Stop Function GR00640 1913 3076 GLUTAMATE N-ACETYLTRANSFERASE (EC 2.3.1.35) AMINO-ACID ACETYLTRANSFERASE (EC 2.3.1.1) GR00640 3125 4075 ACETYLGLUTAMATE KINASE (EC 2.7.2.8) WV0122 14106 13327 N-ACETYL-GAMMA-GLUTAMYL-PHOSPHATE REDUCTASE (EC 1.2.1.38) GR00640 757 1536 N-ACETYLGLUTAMATE-5-SEMIALDEHYDE DEHYDROGENASE GR0D640 1536 1826 N-ACETYLGLUTAMATE-5-SEMIALDEHYDE DEI-YDROGENASE GR00640 4079 5251 ACETYLORNITHINE AMINOTRANSFERASE (EC 2.6.1.11) ACETYLORNITHINE AMINOTRANSFERASE (EC 2.6. 1.11) GR00287 4714 5943 ACETYLORNITHINE AMINOTRANSFERASE (EC 2.6. 1.11) GR00640 5268 6224 ORNITHINE CARBAMOYLTRANSFERASE (EC 2.1.3.3) GR00640 6914 8116 ARGININOSUCCI NATE SYNTHASE (EC 6.3.4.5) WV0122 6683 5253 ARGININOSUCCINATE LYASE (EC 4.3.2.1) GR00640 8180 8962 ARGININOSUCCINATE LYASE (EC 4.3.2.1) GR00640 8949 9611 ARGININOSUCCINATE LYASE (EC 4.3.2.1) GR00654 32291 33436 ORNITHINE CYCLODEAMINASE (EC 4.3.1.12) GR00032 19289 20230 SPERMIDINE SYNTHASE (EC 2.5.1.16) GR00424 12652 14190 SPERMIDINE SYNTHASE (EC 2.5.1.16) GR00498 2942 2142 PUTRESCINE OXIDASE (EC 1.4.3.10) GR00640 6231 6743 ARGININE HYDROXIMATE RESISTANCE PROTEIN WV0122 13327 13037 N-ACETYL-GAMMA-GLUTAMYL-PHOSPHATE REDUCTASE (EC 1.2.1.38) CARBAMOYL-PHOSPHATE SYNTHASE SMALL CHAIN (EC 6.3.5.5) N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) Table 1 (continued) NT Start NT Stop Function Nucleic Acid SEQ ID NO 361 363 365 367 369 371 373 Amino Acid Identification Code SEQ ID NO 362 RXS00907 364 RXS02001 366 RXS02101 368 RXS02234 370 F RXA02234 372 RXS02565 374 RXS02937 Contig N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) CARBAMOYL-PHOSPHATE SYNTHASE LARGE CHAIN (EC 6.3.5.5) GRO0654 1 3198 CARBAMOYL-PHOSPHATE SYNTHASE LARGE CHAIN (EC 6.3.5.5) N-ACYL-L-AMINO ACID AMIDOHYDROLASE (EC 3.5.1.14) N-ACYL-L-AMINO ACID ANIDOHYDROLASE (EC 3.5.1.14) Histidine metabolism Nucleic Acid SEQ ID NO 375 377 379 381 Amino Acid Identification Code SEQ ID NO 376 RXA02194 378 RXA02195 380 RXA01097 382 RXA01 100 RXA01 101 RXN01657 F RXA01657 RXA01098 RXN01 104 F RXA01 104 RXN00446 F RXA00446 RXAO1 105 RXA01 106 RXC00930 RXCO1 096 RXCO 1656 RXCO 1158 GROO645 GRO0645 GROO306 GR00306 GR00306 VVO1 0 GRO0460 GR00306 W0059 GR00306 W01 12 GROO108 GR00306 GROO306 2897 3186 4726 7072 7726 39950 2444 5499 7037 10927 24181 4 12044 13378 2055 2917 4373 6335 7094 39351 2944 4726 6432 10322 23318 525 10947 12053 ATP PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.17) PHOSPHORIBOSYL-ATP PYROPHOSPHOHYDROLASE (EC 3.6.1.31) PHOSPHORIBOSYL-AMP CYCLOHYDROLASE (EC 3.5.4.19) PHOSPHORIBOSYLFORMIMINO-5-AMINOIMIDAZOLE CARBOXAMIDE RIBOTIDE ISOMERASE (EC 5.3.1.16) AMIDOTRANSFERASE HISH (EC AMIDOTRANSFERASE HISH (EC AMIDOTRANSFERASE HISH (EC HISF PROTEIN IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE (EC 4.2.1.19) IMIDAZOLEGLYCEROL-PHOSPHATE DEHYDRATASE (EC 4.2.1.19)/ HISTIDINOL-PHOSPIIATASE (EC 3.1.3.15) HISTIDINOL-PHOSPHATE AMINOTRANSFERASE (EC 2.6.1.9) HISTIDINOL-PHOSPHATE AMINOTRANSFERASE (EC 2.6.1.9) HISTIDINOL-PHOSPHATE AMINOTRANSFERASE (EC 2.6.1.9) HISTIDINOL DEHYDROGENASE (EC 1.1.1.23) PROTEIN INVOLVED IN HISTIDINE METABOLISM PROTEIN INVOLVED IN HISTIDINE METABOLISM PROTEIN INVOLVED IN HISTIDINE METABOLISM MEMBRANE SPANNING PROTEIN INVOLVED IN HISTIDINE METABOLISM NT Start NT Stop Function Metabolism of aromatic amino acids Nucleic Acid SEQ ID NO 411 413 415 417 419 421 Amino Acid identification Code Cntg SEQ ID NO 412 RXA02458 GR00712 414 RXA02790 GR00777 416 RXN00954 W0247 418 F RXA00954 GR00263 420 RXN00957 W0208 422 F RXA00957 GR00264 NT Start NT Stop Function 3056 5806 3197 3 1211 3 4345 6948 2577 590 2764 1130 3-PHOSPHOSHIKIMATE 1 -CARBOXYVINYLTRANSFERASE (EC 2.5.1.19) 4-AM INO-4-DEOXYCHORISMATE LYASE (EC ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.18) ANTHRANILATE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.18) ANTI-RANILATE SYNTHASE COMPONENT I (EC 4.1.3.27) ANTHRANILATE SYNTHASE COMPONENT I (EC 4.1.3.27) Nucleic Acid SEQ ID NO 423 425 427 429 431 433 435 437 439 441 "43 447 "49 451 453 455 457 459 461 Amino Acid SEQ ID NO 424 426 428 430 432 434 436 438 440 442 4"4 446 448 450 452 454 456 458 460 462 Identification Code RXA02687 RXN01 698 F RXA01698 RXA01 095 RXA00955 RXA02814 RXA00229 RXA02093 RXA02791 RXA01 699 RXA00952 RXN00956 F RXA00956 RXAOOO64 RXN00448 F RXA00448 F RXA00452 RXA00564 RXA00579 RXA00958 RXN03007 RXN02918 RXNO01116 RXNO01115 RXS001 16 F RXAOO1 16 RXS00391 RXS00393 F RXA00393 RXS00-446 F RXA00446 RXS0061 8 F RXA00618 F RXA00627 RXS01105 RXS02315 RXS02550 RXS0231 9 RXS02908 RXS03003 RXS03026 Table 1 (continued) Contig. NT Start NT Stop Function GR00754 11306 12250 CHORISMATE MUTASE (EC 5.4.99.5) /PREPHENATE DEHYDRATASE (EC 4.2.1. 51) W0134 11507 12736 CHORISMATE SYNTHASE (EC 4.6.1.4) GR0D477 2 991 CHORISMATE SYNTHASE (EC 4.6.1.4) GR00306 3603 2821 INDOLE-3-GLYCEROL PHOSPHATE SYNTI-ASE (EC 4.1.1.48) GR00263 586 2007 INDOLE-3-GLYCEROL PHOSPHATE SYNTHASE (EC 4.1.1.48)/N-{5-PHOSPHO- RIBOSYL)ANTHRANILATE ISOMERASE (EC 5.3.1.24) GR00795 598 128 ISOCHORISMATE MUTASE GR00033 1715 936 SHIKIMATE 5-DEHYDROGENASE (EC 1.1.1.25) GR00629 12444 13247 SHIKIMATE 5-OEHYDROGENASE (EC 1.1.1.25) GR00777 6968 7795 SHIKIMATE 5-DEHYDROGENASE (EC 1.1.1.25) GR00477 984 1553 SHIKIMATE KINASE (EC 2.7.1.71) GR00262 97 936 TRYPTOPHAN SYNTHASE ALPHA CHAIN (EC 4.2.1.20) W0247 1140 4 TRYPTOPHAN SYNTHASE BETA CHAIN (EC 4.2.1.20) GR00263 2027 3157 TRYPTOPHAN SYNTHASE BETA CHAIN (EC 4.2.1.20) GROOO1O 2499 3776 TYROSINE AMINOTRANSFERASE (EC 2.6.1.5) W01 12 33959 32940 PREPHENATE DEHYDROGENASE (EC 1.3.1.12) GROO109 3 668 PREPHENATE DEHYDROGENASE (EC 1.3.1.12) GROO110 854 1099 PREPHENATE DEHYDROGENASE (EC 1.3.1.12) GROO156 11384 10260 PHOSPHO-2-DEHYDRO-3-DEOXYHEPTONATE ALDOLASE (EC 4.1.2.15) G3R00156 5946 4087 PARA-AMINOBENZOATE SYNTHASE COMPONENT I (EC GR00264 1130 1753 PARA-AMINOBENZOATE SYNTHASE GLUTAMINE AMIDOTRANSFERASE COMPONENT 11 (EC ANTHRANILATE SYNTHASE COMPONENT 11 (EC 4.1.3.27) W0208 3410 3778 ANTHRANILATE SYNTHASE COMPONENT 11 (EC 4.1.3.27) W0086 25447 25887 TRYPTOPHAN SYNTHASE BETA CHAIN (EC 4.2.1.20) W0182 7497 6886 3-OXOADIPATE COA-TRANSFERASE SUBUNIT B (EC 2.8.3.6) W0182 10347 11099 3-OXOADIPATE ENOL-LACTONE HYDROLASE (EC 3.1.1.24) /4-

CARBOXYMUCONOLACTONE

ASPARTATE AMINOTRANSFERASE (EC 2.6.1. 1) GROD018 510 4 ASPARTATE AMINOTRANSFERASE (EC 2.6.1.1) O-SUCCINYLBENZOIC ACID-COA LIGASE (EC 6.2.1.26) 1,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE (EC GR00086 4030 4911 1 ,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE (EC HISTIDINOL-PHOSPHATE AMINOTRANSFERASE (EC 2.6.1.9) GRO0108 4 525 HISTIDINOL-PHOSPHATE AMINOTRANSFERASE (EC 2.6.1.9) ASPARTATE AMINOTRANSFERASE (EC 2.6.1.1) GR00163 213 746 ASPARTATE AMINOTRANSFERASE (EC 2.6.1.1) GR00164 854 1138 ASPARTATE AMINOTRANSFERASE (EC 2.6.1.1) HISTIDINOL-PHOSPHATE AMINOTRANSFERASE (EC 2.6.1.9) 2-SUCCINYL-6-HYDROXY-2,4-CYCLOHEXADIENE-1-CARBOXYLATE SYNTHASE 2-OXOGLUTARATE DECARBOXYLASE (EC 4.1.1.71) ASPARTATE AMINOTRANSFERASE (EC 2.6.1.1) NAPHTHOATE SYNTHASE (EC 4.1.3.36) O-SUICCINYLBENZOIC ACID-COA LIGASE (EC 6.2.1.26) ASPARTATE AMINOTRANSFERASE (EC 2.6.1.1) 3-DEHYDROQUINATE DEHYDRATASE (EC 4.2.1.10) Nucleic Acid Amino Acid SEQ ID NO SEQ ID NO 505 506 507 508 509 510 511 512 513 514 Identification Code Cot.

RXS03074 RXCO1434 RXC02080 RXC02789 RXC02295 Table 1 (continued) NT Start NT Stop Function S-ADENOSYLMETHIONINE:2-DEMETHYLMENAQUINONE METHYLTRANSFERASE (EC MEMBRANE SPANNING PROTEIN INVOLVED IN METABOLISM OF AROMATIC AMINO ACIDS AND RIBOFLAN MEMBRANE SPANNING PROTEIN INVOLVED IN METABOLISM OF AROMATIC AMINO ACIDS CYTOSOLIC PROTEIN INVOLVED IN METABOLISM OF AROMATIC AMINO

ACIDS

MEMBRANE SPANNING PROTEIN INVOLVED IN METABOLISM OF AROMATIC AMINO ACIDS Aminobutyrate metabolism Nucleic Add SEQ ID NO 515 517 519 Amino Acid SEQ ID NO 516 518 520 Identification Code RXN03063 RXN02970 F RXA0OD9 W0035 W0021 GR00287 NT Start 666 4714 4714 NT Stop 1697 6081 5943 Function 4-aminobutyrate amninotransferase (EC 2.6. 1.19) ACETYLORNITHINE AMINOTRANSFERASE (EC 2.6.1.11) ACETYLORNITHINE AMINOTRANSFERASE (EC 2.6.1.11) Vitamins, vitamin-like substances (cofactors), nutraceuticals Thiamine metabolism Nucleic Acid SEQ ID NO 521 523 525 527 529 531 533 535 537 539 541 543 545 547 549 551 Amino Acd SEQ ID NO 522 524 526 528 530 532 534 536 538 540 542 544 546 548 550 552 Identification Code RXA01551 RXA01 019 RXAO1 352 RXA01 381 RXA01 360 RXA01 361 RXA01 208 RXA00838 RXA02400 RXN01209 F RXA01209 RXN0141 3 RXN0161 7 F RXA01617 RXS0 1807 RXCO1021 GR00431 GR00291 GR00393 GR00403 GR00394 GR00394 GR00348 GR00227 GR00699 W0270 GR00348 W0050 W0050 GROO451 NT Start 2945 6 609 3206 162 983 229 1532 1988 1019 1019 27306 22187 2 NT Stop_ 4819 995 4 2286 4 378 1032 633 2557 2446 2446 27905 22858 616 Function THIAMIN BIOSYNTHESIS PROTEIN TH-IC.

THIAMIN-MONOPHOSPHATE KINASE (EC 2.7.4.16) THIAMIN-PHOSPHATE PYROPHOSPHORYLASE (EC 2.5.1.3) THIF PROTEIN THIG PROTEIN THIG PROTEIN HYDROXYETHYLTHLAZOLE KINASE (EC 2.7.1.50) APBA PROTEIN THIAMIN BIOSYNTHESIS PROTEIN X PHOSPHOMETHYLPYRIMIDINE KINASE (EC 2.7.4.7) PHOSPHOMETHYLPYRIMIDINE KINASE (EC 2.7.4.7) PHOSPHOMETHYLPYRIMIDINE KINASE (EC 2.7.4.7) PHOSPHOMETHYLPYRIMIDINE KINASE (EC 2.7.4.7) PHOSPHOMETHYLPYRIMIDINE KINASE (EC 2.7.4.7) PYRIDOXINE KINASE (EC 2.7.1.35) CYTOSOLIC KINASE INVOLVED IN METABOLISM OF SUGARS AND THIAMIN Table 1 (continued) Riboflavin metabolism Nucleic Acid Amino Acid Identification Code Contig NT Start NT Stop Function SEQ ID NO SEQ ID NO 553 554 RXN02246 WV0130 4388 5371 diamlnohydroxyphosphoribosylaminopyrimidtne deamninase (EC 3.5.4.26)1 5-amino- 0 reductase (EC 1.1.1.193) 555 556 F RXA02246 GR00654 14299 15282 RIBG PROTEIN nibofiavin-specific deamninase 557 558 RXA02247 GR00654 15286 15918 RIBOFLAVIN SYNTHASE ALPHA CHAIN (EC 2.5.1.9) 559 560 RXN02248 WV0130 6021 7286 GTP CYCLOHYDROLASE 11 (EC 3.5.4.25) 3,4-DIHYDROXY-2-BUTANONE 4- PHOSPHATE SYNTHASE 561 562 F RXA02248 GR00654 15932 17197 RIBA PROTEIN GTP cydlohydrolase 11 [EC:3.5.4.25] 563 564 RXN02249 WV0130 7301 7777 6,7-DIMETHYL48-RIBITYLLUMAZINE SYNTHASE (EC 2.5.1.9) 565 566 F RXA02249 GR00654 17212 17688 RIBH PROTEIN 6,7-dimethyl-8-ribityflumazine synthase (dmrl synthase, tumnazine synthase, riboflavin synthase beta chain) [EC:2.5.1.91 567 568 RXA02250 GR00654 17778 18356 RIBX PROTEIN 569 570 RXA01489 GR00423 3410 2388 RIBOFLAVIN KINASE (EC 2.7.1.26) FMN ADENYLYLTRANSFERASE (EC 2.7.7.2) 571 572 RXA02135 GR00639 2809 1736 NICOTINATE-NUCLEOTIDE-DIMETHYLBENZIMIDAZOLE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.21) 573 574 RXA01489 GR00423 3410 2388 RIBOFLAVIN KINASE (EC 2.7.1.26) FMN ADENYLYLTRANSFERASE (EC 2.7.7.2) 575 576 RXNO1 712 WV0191 8993 8298 RIBOFLAVIN-SPECIFIC DEAMINASE (EC 577 578 F RXA01712 GR0D484 2652 2152 RIBOFLAVIN-SPECIFIC DEAMINASE (EC 579 580 RXN02384 WV0213 1386 679 ALPHA-RIBAZOLE-5-PHOSPHATE PHOSPHATASE (EC 581 582 RXN01560 WV0319 767 438 RIBOFLAVIN-SPECIFIC DEAMINASE (EC 0 583 584 RXN00667 W01I09 1363 350 DRAP DEAMINASE 585 586 RXCO1711 MEMBRANE SPANNING PROTEIN INVOLVED IN RIBOFLAVIN METABOLISM 587 588 RXCO2380 PROTEIN INVOLVED IN RIBOFLAVIN METABOLISM 589 590 F RXA02380 GR00691 709 56 Predicted nucleotidyftransferases 591 592 RXC02921 CYTOSOLIC PROTEIN INVOLVED IN METABOLISM OF RIBOFLAVIN AND

LIPIDS

593 594 RXCO1 434 MEMBRANE SPANNING PROTEIN INVOLVED IN METABOLISM OF AROMATIC AMINO ACIDS AND RIBOFLAVIN Vitamin B36 metabolism Nucleic Acid Amino Acid Identification Code Contig. NT Start NT Stop Function SEQ ID NO SEQ ID NO 595 596 RXA01807 GRO0509 7868 7077 PYRIDOXINE KINASE (EC 2.7.1.35), pyridoxallpyridoxinelpyridoxamine kinase Table I (continued) Nicotinate (nicotinic acid), nicotinamide, NAD and NADP Nudeic Acid Amino Acid SEQ ID NO SEQ IDNO 597 598 599 600 601 602 603 604 605 606 NAD Biosynthesis Identification Code Cont NT Start NT Stop Function RXN02754 F RXA02405 F RXA02754 RXA021 12 RXA021 11 WV0084 22564 23901 NICOTINATE PIIOSPHORIBOSYLTRANSFERASE (EC 2.4.2.11) GROO701 774 4 NICOTINATE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.11) GR00766 3 488 NICOTINATE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.11) GR00632 5600 6436 NICOTINATE-NUCLEOTIDE PYROPHOSPHORYLASE (CARBOXYLATING) (EC 2.4.2.19) GROO632 4310 5593 QUINOLINATE SYNTHETASE A Nucleic Acid SEQ ID NO 607 609 Amino Acid SEQ ID NO 608 610 Identification Code Contig. NT Start NT Stop Function RXA01073 RXN02754 GR00300 1274 2104 NH(3)-DEPENDENT NAD(+) SYNTHETASE (EC 6.3.5.1) W00D84 22564 23901 NICOTINATE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.11) Pantothenate and Coenzyme A (CoA) biosynthesis Nucleic Acid SEQ ID NO 611 613 615 Amino Acid SEQ ID NO 612 614 616 Identific-ation Code Cot. NT Start NT Stop RXA02299 RXA0 1928 RXN01929 F RXAO1 929 RXA01521 RXS01145 F RXAO1 145 RXA02239 RXA00581 RXS00838 RXCO2238 GR00662 GR00555 W01 27 GR00555 GR00424 GR00321 GR00654 GROO156 10452 1957 47590 2766 25167 1075 5784 7572 10859 1121 48402 1960 25964 1530 7049 8540

ON

Function 0 ASPARTATE 1-DECARBOXYLASE PRECURSOR (EC 4.1.1.11) PANTOATE-BETA-ALANINE LIGASE (EC 6.3.2.1) 3-METHYL-2-OXOBUTANOATE HYDROXYMETHYLTRANSFERASE (EC 2.1.2.11) DECARBOXYLASE (EC 4.1.1.44) 3-METHYL-2-OXOBUTANOATE HYOROXYMETHYLTRANSFERASE (EC 2.1.2.11) PANTOATE-BETA-ALANINE LIGASE (EC 6.3.2.1) KETOL-ACID REDUCTOISOMERASE (EC 1.1.1.86) KETOL-ACID REDUCTOISOMERASE (EC 1.1.1.86) DNAIPANTOTHENATE METABOLISM FLAVOPROTEIN PANTOTHENATE KINASE (EC 2.7.1.33) 2-DEHYDROPANTOATE 2-REDUCTASE (EC 1.1.1.169) PROTEIN INVOLVED IN METABOLISM OF S-AOENOSYLMETHIONINE, PURINES AND PANTOTHENATE Biotin metabolism Nucleic Acid Amino Acid SEQ ID NO SEQ ID NO 633. 634 Identification Code Cot. NT Start NT Stop Function RXN03058 RX008 W0028 8272 8754 BIOTIN SYNTHESIS PROTEIN BlOC Table 1 (continued) NT Start NT Stop Function Nucleic Acid SEQ ID NO 635 637 639 Amino Acid SEQ ID NO 636 638 640 Identification Code F RXA02903 RXAG0166 RXA00633 RXA00632 RXA00295 RXA00223 RXN00262 F RXA00262 RXNO0435 F RXA00435 F RXA02801 RXA02516 RXA02517 GRIOD40 GROO025 GR00166 GR00166 GR00047 GRO0032 WV0123 GROO040 WV0112 GROWl00 GR00782 GR00723 GROO723 11532 3650 3556 2281 3407 23967 16681 79 10037 3563 438 1724 2989 12014 4309 2288 1610 4408 22879 15608 897 11209 2949 4 2986 3435 BIOTIN SYNTHESIS PROTEIN BlOC BIOTIN SYNTHESIS PROTEIN BlOC ADENOSYLMETHIONINE-8-AMINO-7-OXONONANOATE AMINOTRANSFE RASE (EC 2.6.1.62) DETHIOBIOTiN SYNTHETASE (EC 6.3.3.3) BIOTIN SYNTHASE (EC 2.8.1.6) NIFS PROTEIN NIFS PROTEIN NIFS PROTEIN NIFS PROTEIN NIFS PROTEIN NIFS PROTEIN NIFS PROTEIN NIFU PROTEIN Lipoic Acid Nucleic Acid Amino Acid Identification Code SEQ ID NO SEQ IDNO 661 662 RXA01747 663 664 RXA01746 665 666 RXA02106 667 668 R.XS0l 183 Contig GROO495 GROD495 GROO632 NT Start NT Stop Function 2506 1614 472 3549 LIPOIC ACID SYNTHETASE 2366 LIPOATE-PROTEIN LIGASE B (EC 1527 LIPOATE-PROTEiN LIGASE A (EC DIHYDROLIPOAMIDE SUCCINYLTRANSFERASE COMPONENT (E2) OF 2- OXOGLUTARATE DEHYDROGENASE COMPLEX (EC 2.3.1.61) LIPOAMIDE DEHYDROGENASE COMPONENT (E3) OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX (EC 1.8.1.4) LIPOAMIDE DEHYDROGENASE COMPONENT (123) OF BRANCHED-CHAIN ALPHA-KETO ACID DEHYDROGENASE COMPLEX (EC 1.8.1.4) 669 670 RX50 1260 RXS01261 Folate biosynthesis Nucleic Acid Amino Acid Identification Code SEQ ID NO SEQ ID NO 673 674 RXA02717 675 676 RXN02027 677 678 F RXA02027 679 680 RXA00106 681 682 RXN01321 683 684 F RXA01321 685 686 RXA00461 Coig NT Start NT Stop Function GR00758 18281 17400 5,10-METHYLENETETRAHYDROFOLATE REDUCTASE (EC 1.7.99.5) W0296 503 1003 5-FORMYLTETRAHYDROFOLATE CYCLO-LIGASE (EC 6.3.3.2) GR00616 500 6 5-FORMYLTETRAHYDROFOLATE CYCLO-LiGASE (EC 6.3.3.2) GROO014 17469 17924 DIHYDROFOLATE REDUCTASE (EC 1.5.1.3) W0082 8868 9788 FORMYLTETRAHYDROFOLATE DEFORMYLASE (EC 3.5. 1.10) GROO384 23 559 FORMYLTETRAHYDROFOLATE DEFORMYLASE (EC 3.5.1.10) GROW116 428 1279 METHYLENETIETRAHYDROFOLATE DEHYDROGENASE (EC 1.5.1.5)1 METHENYLTETRAHYDROFOLATE CYCLOHYDROLASE (EC 3.5.4.9) GR00424 20922 21509 GTP CYCLOHYDROLASE I (EC 3.5.4.16) GR00424 22360 22749 DIHYDRONEOPTERIN ALDOLASE (EC 4.1.2.25) 687 688 689 690 RXA01514 RXA01516 Table I (continued) NT Start NT Stop Function Nudleic Acid SEQ ID NO 691 693 695 697 699 701 703 Amino Acid SEQ ID NO 692 694 696 698 700 702 704 Identification Code RXA01 515 RXA02024 RXAGO1O6 RXA00989 RXA01 517 RXA00579 RXA00958 RXA02790 RXA00106 RXN02198 F RXA02198 RXN02085 F RXA02O85 F RXA02086 RXN02648 F RXA02648 F RXA02658 RXS021 97 RXCO0988 RXCO1 518 RXCO1 942 GROO424 GROD613 GROO014 GR00280 GR00424 GR00156 GROO264 GR00777 GROO014 W0302 GR00646 W01 26 GR00629 GRD0629 GR00751 GR00752 21513 4026 17469 2903 22752 5946 1130 5806 17469 9228 2483 8483 3496 5252 5254 14764 22364 4784 17924 1371 23228 4087 1753 6948 17924 11726 6 10717 5295 5731 4730 15447 DIHYDROPTEROATE SYNTHASE (EC 2.5.1.15) DIHYDROPTEROATE SYNTHASE (EC 2.5.1.15) DIHYDROFOLATE REDUCTASE (EC 1.5.1.3) FOLYLPOLYGLUITAMATE SYNTIIASE (EC 6.3.2.17) 2-AMINO-4-HYDROXY-6-HYDROXYMETHYLDIHYDROPTERIDINE PYROPHOSP-OKINASE (EC 2.7.6.3) PARA-AMINOBENZOATE SYNTHASE COMPONENT I (EC PARA-AMINOBENZOATE SYNTHASE GLUTAMINE AMIDOTRANSFERASE COMPONENT 11 (EC ANTHRANILATE SYNTHASE COMPONENT 11 (EC 4.1.3.27) 4-AMINO-4-DEOXYCHORISMATE LYASE (EC DIHYDROFOLATE REDUCTASE (EC 1.5.1.3) 5-METHYLTETRAHYDROFOLATE-HOMOCYSTEINE METHYLTRANSFERASE (EC 2.1.1.13) 5-METHYLTETRAHYDROFOLATE--I-OMOCYSTEINE METHYLTRANSFERASE (EC 2.1.1.13)

METHYLTRANSFERASE

METHYLTRANSFERASE (EC 2.1.1.14) METHYLTRANSFERASE (EC 2.1.1.14) METHYLTRANSFERASE (EC 2.1.1.14) METHYLTRANSFERASE (EC 2.1.1.14) METHYLTRANSFERASE (EC 2.1.1.14) 5-METHYLTETRAHYDROFOLATE-HOMOCYSTEINE METHYLTRANSFERASE (EC 2.1.1.13) PROTEIN INVOLVED IN FOLATE METABOLISM MEMBRANE SPANNING PROTEIN INVOLVED IN FOLATE METABOLISM ATP-BINDING PROTEIN INVOLVED IN FOLATE METABOLISM Molybdopterin Metabolism Nucleic Acid SEQ ID NO 733 735 737 739 741 743 745 747 Amino Acid SEQ ID NO 734 736 738 740 742 7"4 746 748 Identification Code RXN02802 F RXA02802 F RXA00438 RXN00437 F RXA00437 RXNO0439 F RXAO0439 F RXA00442 Contig.

GR00783 GRO01O03 W011 2 GR00103 W011 2 GROO104 GROO105 NT Start 17369 7 362 17824 3 18742 2 830 NT Sto_ 16299 474 796 17369 362 18275 196 1087 Function MOLYBDOPTERIN BIOSYNTHESIS MOEB PROTEIN MOLYBDOPTERIN BIOSYNTHESIS MOEB PROTEIN MOLYBDOPTERIN BIOSYNTHESIS MOEB PROTEIN MOLYBDOPTERIN (MPT) CONVERTING FACTOR, SUBUNIT 2 MOLYBDOPTERIN (MPT) CONVERTING FACTOR, SUBUNIT 2 MOLYBDOPTERIN CO-FACTOR SYNTHESIS PROTEIN MOLYBDOPTERIN CO-FACTOR SYNTHESIS PROTEIN MOLYBDOPTERIN CO-FACTOR SYNTHESIS PROTEIN Nucleic Acid Amnino Acid SEQ ID NO SEQ ID NO 749 750 751 752 753 754 755 756 757 758 759 760 761 762 763 764 765 766 Identification Code RXA00440 RXN00441 F RXA00441 RXN02085 F RXA02085 F RXA02086 RXN02648 F RXA02648 F RXA02658 RXA01516 RXA01515 RXA02024 RXA01 719 RXA01 720 RXS03223 F RXA01970 RXA02629 RXA02318 RXAO1 517 RXN01 304 RXS02556 RXS02560 Contig Table I (continued) NT Start NT Stop Function GRO01O04 196 654 MOLYBDENUM COFACTOR BIOSYNTHESIS PROTEIN GB WV01 12 19942 18779 MOLYBDOPTERIN CO-FACTOR SYNTHESIS PROTEIN GR00105 2 793 MOLYBDOPTERIN CO-FACTOR SYNTHESIS PROTEIN METHYLTRANSFERASE (EC 2.1.1.14) GR00629 3496 5295 METHYLTRANSFERASE (EC 2.1.1.14) CR00629 5252 5731 METHYLTRANSFERASE (EC 2.1.1.14) METHYLTRANSFERASE (EC 2.1.1.14) CR00751 5254 4730 METHYLTRANSFERASE (EC 2.1.1.14) GR00752 14764 15447 METHYLTRANSFERASE (EC 2.1.1.14) GROD424 22360 22749 DIHYDRONEOPTERIN ALDOLASE (EC 4.1.2.25) GR00424 21513 22364 DIHYDROPTEROATE SYNThASE (EC 2.5.1.15) CR00613 4026 4784 DIHYDROPTEROATE SYNTHASE (EC 2.5.1.1 GR00488 1264 704 MOLYBDOPTERIN-GUANINE DINUCLEOTIDE BIOSYNTHESIS PROTEIN A GR00488 2476 1268 MOLYBDOPTERIN BIOSYNTHESIS MOEA PROTEIN MOLYBDOPTERIN BIOSYNTHESIS MOEA PROTEIN GR00568 2 1207 MOLYBDOPTERIN BIOSYNTHESIS MOEA PROTEIN GR00748 1274 690 MOLYBDOPTERIN BIOSYNTHESIS CNXI PROTEIN GR00665 9684 9962 (D90909) pterin-4a-carbinolamnine dehydratase (Synechocystis sp.) GR00424 22752 23228 2-AMINO-4-HYDROXY-6-HYDROXYMETHYLDIHYDROPTERI DINE PYROPHOSPHOKINASE (EC 2.7.6.3) WV0148 4.449 4934 MOLYBDOPTERIN BIOSYNTHESIS MOG PROTEIN FLAVOHEMOPROTEIN DIHYDROPTERIDINE REDUCTASE (EC 1.6.99.7) OXYGEN-INSENSITIVE NAD(P)H NITROREDUCTASE (EC1.--I DIHYDROPTERIDINE REDUCTASE (ECI .6.99.7) Vitamin 1 1,porphyrins and hemne metabolism Nudleic Acid Amino Acid SEQ ID NO SEQ ID NO 793 794 795 796 797 798 799 800 801 802 803 804 805 806 807 808 809 810 811 812 Identification Code Contig. NT Start NT Stop Function RXA00382 RXA00156 RXA00624 RXAOO306 RXA00884 RXN02503 F RXA02503 RXA00377 RXN02504 F RXA02504 GR00082 GR00023 CR00163 GR00051 CR00242 W0007 CR00720 CR00081 W0007 CR00720 2752 10509 7910 2206 10137 22456 16906 1427 22805 17379 1451 9400 8596 1274 11276 22854 17340 306 23362 17816 GLUTAMATE-1-SEMIALDEHYDE 2,1 -AMINOMUTASE (EC 5.4.3.8) FERROCHELATASE (EC 4.99.1.1) FERROCHELATASE (EC 4.99.1.1) HEMK PROTEIN OXYGEN-INDEPENDENT COPROPORPHYRINOGEN III OXIDASE (EC PORPHOBILINOGEN DEAMINASE (EC 4.3.1.8) PORPHOBILINOGEN DEAMINASE (EC 4.3.1.8) UROPORPHYRINOGEN DECARBOXYLASE (EC 4.1.1.37) PORPHOBILINOGEN DEAMINASE (EC 4.3.1.8) PORPHOBILINOGEN DEAMINASE (EC 4.3.1.8) Nucleic Acid Amino Acid SEQ ID NO SEQ ID NO 813 814 815 816 817 818 819 820 821 822 823 824 Identification Code Contig.

Table I (continued) NT Start NT Stop Function RXNO1 162 F RXA01 162 RXA01 692 RXN00371 F RXA00371 F RXAD0374 RXND0383 F RXA00376 F RXA00383 RXA0 1253 RXA021 34 RXA021 35 RXA021 36 RXN031 14 RXN01 810 RXS03205 F RXAOO3D6 RXCO1715 WV0088 GROO330 GR00474 W0226 GR00078 GROO079 W0223 GROO081 GR00082 GROO365 GRD0639 GR00639 GROO639 W0088 W0082 1849 1248 1498 4180 929 1102 4206 287 3876 2536 1721 2809 3362 1 1739 524 PRECORRIN-6Y METHYLASE (EC 4 PRECORRIN-6Y METHYLASE (EC 749 UROPORPHYRIN-111 C-METHYLTRANSFERASE (EC 2.1.1.107) 5973 UROPORPHYRIN-111 C-METHYLTRANSFERASE (EC 2.1.1.107) UROPORPHYRINOGEN-11 SYNTI-ASE (EC 4.2.1.75) 6 UROPORPHYRIN-111 C-METHYLTRANSFERASE (EC 2.1.1.107)1/ UROPORPH-YRINOGEN-111 SYNTHASE (EC 4.2.1.75) 371 UROPORPHYRIN-111 0-METHYLTRANSFERASE (EC 2.1.1.107)1 UROPORPHYRINOGEN-111 SYNTHASE (EC 4.2.1.75) 2863 PROTOPORP-YRINOGEN OXIDASE (EC 1.3.3.4) 6 PROTOPORPHYRINOGEN OXIDASE (EC 1.3.3.4) 2863 PROTOPORPHYRINOGEN OXIDASE (EC 1.3.3.4) 1787 COBYRIC ACID SYNTHASE 801 COB3ALAMvIN (5'-PHOSPHATE) SYNTHASE 1736 NICOTINATE-NUCLEOTIDE-DIMETHYLBENZIMIDAZOLE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.21) 2841 COBINAMIDE KINASE COBINAMIDE PHOSPHATE GUANYLYLTRANSFERASE 552 COBG PROTEIN (EC 663 HEMIN-BINDING, PERIPLASMIC PROTEIN HMUT PRECURSOR HEMK PROTEIN HEMK PROTEIN CYTOSOLIC PROTEIN INVOLVED IN PORPHYRIN METABOLISM Vitamin C precursors Nucleic Acid Amino Acid Identification Code SEQ ID NO SEQ ID NO 849 850 RXN0O420 851 852 F RXA0O420 853 854 F RXAO0426 855 856 RXN00708 857 858 F RXA00708 859 860 RXA02373 861 862 RXS00389 863 864 RXS00419 865 866 RXCOO416 867 868 RXC02206 GROO096 GRD0097 W0ol'5 GR00185 GR00688 NT Start NT Stop Function 2511 1048 L-GULONOLACTONE OXIDASE (EC 1.1.3.8) 2 541 L-GULONOLACTONE OXIDASE (EC 1.1.3.8) 1737 2258 L-GULONOLACTONE OXIDASE (EC 1.1.3.8) 4678 3872 2,5-DIKETO-D-GLUCONIC ACID REDUCTASE (EC 2030 1359 2,5-DIKETO-D-GLUCONIC ACID REDUCTASE (EC 1540 626 2,5-DIKETO-D-GLUCONIC ACID REDUCTASE (EC 111oxogbutarate semnialdehyde dehydrogenase (EC ACETOACETYL-COA REDUCTASE (EC 1.1.1.36) MEMBRANE SPANNING PROTEININVOLVED IN METABOLISM OF VITAMIN C PRECURSORS OXIDOREDUCTASE INVOLVED IN METABOLISM OF VITAMIN C PRECURSORS Vitamin K2 Nucleic Acid SEQ ID NO 869 Amino Acid SEQ ID NO 870 Identification Code ContjI.

RXS03074 NT Start NT Stop Function S.ADENOSYLMETHIONINE:2-DEMETHYLMENAQUINONE METHYLTRANSFERASE (EC Table 1 (continued) NT Start NT Stop Function Nucleic Acid SEQ ID NO 871 873 875 877 879 881 883 Amino AcId Identification Code SEQ ID NO 872 F RXA02906 874 RXA02315 876 RXA02319 878 RXS00393 880 F RXA00393 882 RXA00391 884 RXS02908 Cog.

GR1004A 1142 645 S-ADENOSYLMETHIONINE:2-DEMETHYLMENAQUINONE METHYLTRANSFERASE (EC GR00665 8011 6383 2-SUCCINYL-6-HYDROXY-2,4-CYCLOHEXADIENE-1-CARBOXYLATE SYNTHASE 12-OXOGLUTARATE DECARBOXYLASE (EC 4.1.1.71) GR00665 9977 10933 NAPHTHOATE SYNTHASE (EC 4.1.3.36) 1 ,4-DII-YDROXY-2-NAPI-THOATE OCTAPRENYLTRANSFERASE (EC GROO086 4030 4911 1 ,4-DIHYDROXY-2-NAPHTHOATE OCTAPRENYLTRANSFERASE (EC GROO086 2031 2750 O-SUCCINYLBENZOIC ACID-COA LIGASE (EC 6.2.1.26) O-SUCCINYLBENZOIC ACID-COA LIGASE (EC 6.2.1.26) Ubiquinone biosynthesis Nucleic Acid SEQ ID NO 885 887 889 891 Amino Acid Identification Code Contig.

SEQ ID NO 886 RXA00997 GR00283 888 RXA021 89 GROO642 890 RXA0231 1 GR00665 892 RXN02912 W0135 NT Start NT Stop Function 2389 986 3073 13299 1808 249 2384 12547 3-DEMETHYLUBIQUINONE-9 3-METHYLTRANSFERASE (EC 2.1.1.64) 3-DEMETHYLUBIQUINONE-9 3..METHYLTRANSFERASE (EC 2.1.1.64) 3-DEMETHYLUBIQUINONE-9 3-METHYLTRANSFERASE (EC 2.1.1.64) UBIQUINONEIMENAQUINONE BIOSYNTHESIS METHLYTRANSFERASE UBIE (EC 2. COMA OPERON PROTEIN 2 893 894 RXS00998 Purines and Pyrimidines and other Nucleotides Regulation of purine and pyrimidine biosynthesis pathways Purine metabolism Punne Biosynthesis Nucleic Acid SEQ ID NO 895 897 899 901 903 905 907 Amino Acid Identification Code SEQ ID NO 896 RXA01215 898 RXN00558 900 F RXA00558 902 RXN00626 904 F RXA00629 906 F RXA00626 908 RXA02623 Contig. NT Start NT Stop Function GR00352 1187 213 RIBOSE-PHOSPHATE PYROPHOSP-OKINASE, PRPP syntlietase (EC 2.7.6.1) W0103 8235 9581 AMIDOPHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.14) GR00148 61 501 AMIDOPHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.14) W0135 11624 10362 PHOSPHORIBOSYLAMINE-GLYCINE LIGASE (EC 6.3.4.13) GR00165 1450 1713 PHOSPHORIBOSYLAMINE-GLYCINE LIGASE (EC 6.3.4.13) GROO164 1 780 PHOSPHORIBOSYLAMINE-GLYCINE LIGASE, GARS (EC 6.3.4.13) GR00746 4875 4285 PHOSPHORIBOSYLAMINE-GLYCINE LIGASE (EC 6.3.4.13)!/ PHOSPHORIBOSYLFORMYLGLYCINAMIDINE CYCLO-LIGASE (EC 6.3.3.1)! PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE (EC 2.1.2.2) GR00418 10277 9054 PHOSPHORIBOSYLGLYCINAMIDE FORMYLTRANSFERASE 2 (EC 909 910 RXA01442 Nucleic Acid SEQ ID NO 911 913 915 917 919 921 923 925 927 929 931 933 935 937 939 941 943 Amino Acid SEQ ID NO 912 914 916 918 920 922 924 926 928 930 932 934 936 938 940 942 944 Identification Code RXNDD537 F RXA02805 F RXA00537 F RXA0561 RXA00541 RXA00620 RXN00770 F RXA00557 F RXA00770 RXN02345 F RXA02345 RXN02350 F RXA02346 F RXA02350 RXA01 087 RXA0061 9 RXA02622 Table 1 (continued) NT Start NT Stop Function Contig.

W0103 GR00786 GROW138 GROWl50 GRO01 39 GRO0163 W0103 GRO0147 GR002D4 W0078 GRD0676 VV0078 GR00677 GRO0678 GR003D4 GROO1 63 GROO746 3351 54 23 2 2269 3049 9614 15 7809 4788 1534 8369 127 1120 498 793 4274 5636 638 697 280 2937 3939 10783 818 7495 5984 725 8863 5 911 1373 2220 2715 PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE (EC 6.3.5.3) PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE (EC 6.3.5.3) PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE (EC 6.3.5.3) PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE (EC 6.3.5.3) PHOSPHORIBOSYLFORMYLGLYCINAMIDINE SYNTHASE (EC 6.3.5.3) PHOSPHORIBOSYLAMIDOIMIDAZOLE-SUCCINOCARBOXAMIDE SYNTHASE (EC 6.3.2.6) PHOSPHORIBOSYLFORMYLGLYCINAMIDINE CYCLO-LIGASE (EC 6.3.3.1) PHOSPHORIBOSYLFORMYLGLYCINAMIDINE CYCLO-LIGASE (EC 6.3.3.1) PHOSPHORIBOSYLFORMYLGLYCINAMIDINE CYCLO-UIGASE (EC 6.3.3.1) PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE ATPASE SUBUNIT (EC 4.1.1.21) PHOSP-ORIBOSYLAMINOIMIDAZOLE CARBOXYLASE ATPASE SUBUNIT (EC 4.1.1.21) PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE CATALYTIC SUBUNIT (EC 4.1.1.21) PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE CATALYTIC SUBUNIT (EC 4.1.1.21) PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE CATALYTIC SUBUNIT (EC 4.1.1.21) PHOSPHORIBOSYLAMINOIMIDAZOLE CARBOXYLASE (EC 4.1.1.21) ADENYLOSUCCINATE LYASE (EC 4.3.2.2) PHOSPHORIBOSYLAMINOIMIDAZOLECARBOXAMIDE FORMYLTRANSFERASE (EC 2.1.2.3) IMP CYCLOHYDROLASE (EC 3.5.4.10) GMP, GDP, AMP and ADPR synthesis, from inosine-5'-monophosphate (IMP) Nucleic Acid Amino Acid Identiffication Code Contig. NT Start NT Stop Function SEQ ID NO SEQ ID NO 945 946 RXN00488 W0086 19066 20583 INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE (EC 1.1.1.205) 947 948 F RXA0D492 GR00122 1171 1644 INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE (EC 1.1 .1 .205) 949 950 F RXA0D498 GROO121 1 534 INOSINE-5'-MONOPHOSPHATE DEHYDROGENASE (EC 1.1.1.205) 951 952 RXA02469 GR00715 1927 497 INOSINE-5.-MONOPHOSPHIATE DEHYDROGENASE (EC 1.1.1.205) 953 954 RXN00487 VV0086 23734 25302 GMP SYNTHASE [GLUTAMINE-HYDROLYZING] (EC 6.3.5.2) 955 956 F RXA00487 GROO120 712 2097 GMP SYNTHASE (EC 6.3.4.1) 957 958 RXA02237 GRO0654 4577 5146 GUANYLATE KINASE (EC 2.7.4.8) 959 960 RXA01446 GR00418 17765 16476 ADENYLOSUCCINATE SYNTHETASE (EC 6.3.4.4) 961 962 RXA00619 GR00163 793 2220 ADENYLOSUCCINATE LYASE (EC 4.3.2.2) 963 964 RXA00688 GR00179 10443 10985 ADENYLATE KINASE (EC 2.7.4.3) 965 966 RXA00266 GROOO40 3769 3362 NUCLEOSIDE DIPHOSPHATE KINASE (EC 2.7.4.6) GIAMP degrading activities Nudeic Acid Amino Acid Identification Code Contig.

SEQ ID NO SEQ ID NO 967 968 RXA00489 GRO01: 969 970 RXN02281 W0152 971 972 F RXA02281 GRO06~ Pyrimidine metabolism Pyrimidine biosynthesis de novo: Table I (continued) NT Start NT Stop Function 21 654 1893 59 1101 1775 GMP REDUCTASE (EC 1.6.6.8) 3323 AMP NUCLEOSIDASE (EC 3.2.2.4) 34 AMP NUCLEOSIDASE (EC 3.2.2.4) Nucleic Acid Amino Acid SEQ ID NO SEQ ID NO 973 974 975 976 977 978 979 980 981 982 983 984 985 986 987 988 989 990 991 992 993 994 995 996 997 998 999 1000 1001 1002 1003 1004 1005 1006 1007 1008 1009 1010 1011 1012 1013 1014 1015 1016 1017 1018 Identification Code Contig; NT Start NT Stop Function RXA00147 RXA00 145 RXA00146 RXA02208 RXA01660 RXA02235 RXN01892 F RXA01 892 RXA0105 RXA00131 RXA00266 RXAD071 8 RXA01599 RXN02234 F RXA02234 RXN00450 F RXA0O450 RXN02272 F RXA02272 RXN03004 RXN03137 RXN03171 F RXA02857 GR00022 9722 10900 CARBAMOYL-PHOSPHATE SYNTHASE SMALL CHAIN (EC 6.3.5.5) GR00022 7258 8193 ASPARTATE CARBAMOYLTRANSFERASE CATALYTIC CHAIN (EC 2.1.3.2) GR00022 8249 9589 DIHYDROOROTASE (EC 3.5.2.3) GR00647 2 10D03 DIHYDROOROTATE DEHYDROGENASE (EC 1.3.3.1) GR00462 591 1142 OROTATE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.10) GRO0654 3207 4040 OROTIDINE 5-PHOSPHATE DECARBOXYLASE (EC 4.1.1.23) W0150 3020 3748 URIDYLATE KINASE (EC GR00542 47 775 URIDYLATE KINASE (EC GROO014 16672 17346 THYMIDYLATE SYNTHASE (EC 2.1.1.45) GROO020 7621 7013 THYMIDYLATE KINASE (EC 2.7.4.9) GRODO40 3769 3362 NUCLEOSIDE DIPHOSPHATE KINASE (EC 2.7.4.6) GR00188 4576 5283 CYTIDYLATE KINASE (EC 2.7.4.14) GR00447 8780 10441 CTP SYNTHASE (EC 6.3.4.2) W0134 24708 28046 CARBAMOYL-PHOSPHATE SYNTHASE LARGE CHAIN (EC 6.3.5.5) GR00654 1 3198 CARBAMOYL-PHOSPHATE SYNTHASE LARGE CHAIN (EC 6.3.5.5) W01 12 34491 34814 CYTOSINE DEAMINASE (EC 3.5.4.1) GROW110 322 5 CYTOSINE DEAMINASE (EC 3.5.4.1) W0020 15566 16810 CYTOSINE DEAMINASE (EC 3.5.4.1) GR00655 6691 7935 CREATININE DEAMINASE (EC 3.5.4.21) WV0237 1862 2341 DEOXYCYTIDINE TRIPHOSPHATE DEAMINASE (EC 3.5.4.13) W0129 9680 9579 THYMIDYLATE SYNTHASE (EC 2.1.1.45) W0328 568 1080 URACIL PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.9) GR10003 570 10D82 URACIL PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.9) Table 1 (continued) Punine and pyrimidine base, nucleoside and nucleotide salvage, interconversion, reduction and degradation: Purines: Nucleic Acid Amino Acid Identification Code SEQ ID NO SEQ ID NO 1019 1020 RXA02771 1021 1022 RXA01512 1023 1024 RXA02031 1025 1026 RXA00981 1027 1028 RXN02772 1029 1030 F RXA02772 1031 1032 F RXA02773 1033 1034 RXA01835 1035 1036 RXA01483 1037 1038 RXNO1O27 1039 1040 F RXA01024 1041 1042 F RXAO1 027 10D43 1044 RXA01 528 1045 1046 RXA00072 1047 1048 RXAO1 878 1049 1050 RXN02281 1051 1052 F RXA02281 1053 1054 RXN01 240 1055 1056 RXN02008 Pyrimdine and punine metabolism: Nucleic Acid Amino Acid Identification Code SEQ ID NO SEQ ID NO 1057 1058 RXN01940 1059 1060 F RXA01940 1061 1062 RXA02559 1063 1064 RXA02497 1065 1066 RXN01079 1067 1068 F RXA01079 1069 1070 F RXA01084 1071 1072 RXN01 920 1073 1074 F RXA01920 1075 1076 RXA01080 1077 1078 RXA00867 1079 1080 RXA01416 1081 1082 RXA01486 GR00772 GR00424 GR00618 CR00276 W0171 GR00772 GR00772 GR00517 GR00422 W0143 GR00293 GR00294 GR0D425 GROD012 GR00537 WV0152 GR00659 W01 71 W01 20 GR00557 GR00731 GR00720 W0084 GR00301 GR00302 WV0084 CR00550 CR00301 GR00237 CR00413 CR00423 NT Start 1329 17633 3820 3388 2045 1962 2741 3147 19511 5761 661 2580 5653 446 1239 1893 1101 30442 1138 NT Start 10268- 3 5418 10059 38084 693 3402 32843 1321 1240 1 2 660 NT Stop 1883 18232 3347 4017 1011 2741 2902 3677 18240 6768 5 2347 5126 6 2117 3323 34 29420 5 NT Stop 9333 581 6320 10985 35982 4 2062 31842 908 797 627 631 4 Function ADENINE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.7) HYPOXANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.8) XANTHINE-GUANINE PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.22) CTP PYROPHOSPHOKINASE (EC 2.7.6.5) GUANOSINE-3,5-BIS(DIPHOSPHATE) 3'-PYROPHOSPHOHYDROLASE (EC 3.1.7.2) CUANOSINE.3,5-BIS(DIPHOSPHATE) 3-PYROPHOSPHOHYDROLASE (EC 3.1.7.2) CUANOSINE-3,5'-IS(DIPHOSPHATE) 3-PYROPHOSPHOHYDROLASE (EC 3.1.7.2) GUANOSINE-3'.5'-8IS(DIPHOSPHATE) 3-PYROPHOSPHOHYDROLASE (EC 3.1.7.2) DEOXYCUANOSINETRIPHOSPHATE TRIPHOSPHOHYDROLASE (EC 3.1.5.1) DIADENOSINE 5'.5--PI,P4-TETRAPHOSPHATE HYDROLASE (EC 3.6.1.1 7) DIADENOSINE 5,5-P,P4-TETRAPHOSPHATE H-YDROLASE (EC 3.6.1.17) DIADENOSINE 5*.5"-P1,P4-TETRAPHOSPHATE HYDROLASE (EC 3.6.1.17) DIADENOSINE 5',5".PI,PA-TETRAPHOSPHATE HYDROLASE (EC 3.6.1.17) PHOSPHOADENOSINE PHOSPHOSULFATE REDUCTASE (EC 1.8.99.4) DIMETHYLADENOSINE TRANSFERASE (EC 2. 1. AMP NUCLEOSIDASE (EC 3.2.2.4) AMP NUCLEOSIDASE (EC 3.2.2.4) CTP PYROP-IOSPHOKINASE (EC 2.7.6.5) GUANOSINE-3',5'-BIS(DIPHOSPHATE) 3-PYROPHOSPHOHYDROLASE (EC 3.1.7.2) Function INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE (EC 3.2.2.1) INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE (EC 3.2.2.1) INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE (EC 3.2.2.1) EXOPOLYPHOSPHATASE (EC 3.6.1.11) RIBONUICLEOSIDE-DIPHOSPHATE REDUCTASE ALPHA CHAIN (EC 1. 17.4. 1) RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE ALPHA CHAIN (EC 1.17.4.1) RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE ALPHA CHAIN (EC 1.17.4.1) RIBONUCLEOSIDE-DIPHOSPHATE REDUCTASE 2 BETA CHAIN (EC 1.17.4.1) RIBONUCLEOTIDE REDUCTASE SUBUNIT R2F NRDI PROTEIN POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE (EC 2.7.7.8) POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE (EC 2.7.7.8) POLYRIBONUCLEOTIDE NUCLEOTIDYLTRANSFERASE (EC 2.7.7.8) Table I (continued) Nudeic Acid Amino Acid Identification Code Contig. NT Start NT Stop Function SEQ ID NO SEQ ID NO 0 1083 1084 RXA01678 GR00467 7162 7689 2',3'-CYCLIC-NUCLEOTIDE 2.PHOSPIODIESTERASE (EC 3.1.4.16) 1085 1086 RXA01679 GR00467 7729 8964 2'3'-CYCLIC-NUC-LEOTIDE 2'-PH-OSPIIODIESTERASE (EC 3.1.4.16) 1087 1088 RXN01488 W0139 39842 40789 INOSINE-URIDINE PREFERRING NUCLEOSIDE HYDROLASE (EC 3.2.2.1) 108 190 RXCO0540 CYTOSOLIC PROTEIN INVOLVED IN PURINE METABOLISM 1091 1092 RXC00560 PROTEIN INVOLVED IN PURINE METABOLISM 1093 1094 RXCOI 088 CYTOSOLIC PROTEIN INVOLVED IN PURINE METABOLISM 1095 1096 RXC02624 MEMBRANE SPANNING PROTEIN INVOLVED IN PURINE METABOLISM 1097 1098 RXC02665 PROTEIN INVOLVED IN PURINE METABOLISM 1099 1100 RXC02770 LIPOPROTEIN INVOLVED IN PURINE METABOLISM 1101 1102 RXCO2238 PROTEIN INVOLVED IN METABOLISM OF S-ADENOSYLMvETH-IONINE, PURINES AND PANTOTHENATE 1103 1104 RXCO1946 ABC TRANSPORTER ATP-BINDING PROTEIN INVOLVED IN PURINE

METABOLISM

Pyrimdines: Nudeic Acid Amino Acid Identification Code Cti. NT Start NT Stop Function SEQ ID NO SEQ ID NO 1105 1106 RXN03171 W0328 568 1080 URACIL PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.9) 1107 1108 F RXA02857 GRIO003 570 1082 URACIL PHOSPHORIBOSYLTRANSFERASE (EC 2.4.2.9) 1109 1110 RXN00450 W0112 34491 34814 CYTOSINE DEAMvINASE (EC 3.5.4.1) 1111 1112 F RXAOD450 GRO01l10 322 5 CYTOSINE DEAMINASE (EC 3.5.4.1) 1113 1114 RXA00465 GROW117 337 828 CYTOSINE DEAMINASE (EC 3.5.4.1) 1115 1116 RXA00717 GROW188 3617 4576 RIBOSOMAL LARGE SUBUNIT PSEUDOURIDINE SYNTHASE B (EC 4.2.1.70) 1117 1118 RXA01894 GR00542 1622 2476 PHOSP-ATIDATE CYTIDYLYLTRANSFERASE (EC 2.7.7.41) 1119 1120 RXA02536 GR00726 8581 7826 BETA-UREIDOPROPIONASE (EC 3.5.1.6) 1121 1122 RXN01209 W0270 1019 2446 PHOSPHOMETHYLPYRIMIDINE KINASE (EC 2.7.4.7) 1123 1124 F RXA01209 GR00348 1019 2446 PHOSPHOMETHYLPYRIMIDINE KINASE (EC 2.7.4.7) 1125 1126 RXN01617 WOOSO 22187 22858 PHOSPHOMETHYLPYRIMIDINE KINASE (EC 2.7.4.7) 1127 1128 F RXA01617 GRO0451 2 616 PHOSPHOMETHYLPYRIMIDINE KINASE (EC 2.7.4.7) 1129 1130 RXCO1600 CYTOSOLIC PROTEIN INVOLVED IN PYRIMIDINE METABOLISM 1131 1132 RXC01622 CYTOSOLIC PROTEIN INVOLVED IN PYRIMIDINE METABOLISM 1133 1134 RXCO0128 EXPORTED PROTEIN INVOLVED IN METABOLISM OF PYRIDIMES AND

ADENOSYLIHOMOCYSTEINE

1135 1136 RX001709 CYTOSOLIC PROTEIN INVOLVED IN PYRIMIDINE METABOLISM 1137 1138 RXC02207 EXPORTED PROTEIN INVOLVED IN PYRIMIDINE METABOLISM Table I (continued) Sugars Treha lose Nucleic Acid Amino Acid Identification Code oni. NT Start NT Stop Function SEQ ID NO SEQ ID NO 1139 1140 RXA00347 GROO065 246 1013 TREHALOSE-PHOSPHATASE (EC 3.1.3.12) 1141 1142 RXN01239 W0090 32921 30489 mahtooligosyftrehalose synthase 1143 1144 F RXA01239 GR00358 5147 7579 maftooligosyltrehalose synthase 1145 1146 RXA02645 GR00751 714 2543 maltooligosyltrehalose, trehalohydrolase 1147 1148 RXN02355 VV0051 735 4 TREHALOSE/MALTOSE BINDING PROTEIN 1149 1150 RXN02909 W0135 38532 39017 Hypothetical Trehalose-Binding Protein 1151 1152 RXS00349 Hypothetical Trehalose Transport Protein 1153 1154 RXS03183 TREHALOSE/MALTOSE BINDING PROTEIN 1155 1156 RXCO0874 TRANSMEBRANE PROTEIN INVOLVED IN TREHALOSE METABOLISM 2 Excluded Genes GenBank~m Gene Name Gene Function Reference Accession No.

A09073 ppg Phosphoenol pyruvate carboxylase Bachmann, B. et al. "DNA fragment coding for phosphoenolpyruvat corboxylase, recombinant DNA carrying said fragment, strains carrying the recombinant DNA and method for producing L-aminino acids using said Patent: EP 0358940-A 3 03/21/90 A45579, Threonine dehydratase Moeckel, B. et al. "Production of L-isoleucine by means of recombinant A45581, micro-organisms with deregulated threonine dehydratase," Patent: WO A45583, 9519442-A 5 07/20/95 A45585 ABO03132 murC; ftsQ; ftsZ Kobayashi, M. et al. "Cloning, sequencing, and characterization of the RtsZ gene from coryneform bacteria," Biochem. Biophys. Res. Commun., 236(2):383-388 (1997) ABO 15023 murC; ftsQ Wachi, M. et al. "A murC gene from Coryneform bacteria," Appi. Microbiol.

51 (2).223-228 (1999) AB018530 dtsR Kimura, E. et al. "Molecular cloning of a novel gene, dtsft, which rescues the detergent sensitivity of a mutant derived from Brevibacierium ____________lactofermentum," Biosci. Biolechnol. Biochem., 60(10): 1565-1570 (1996) AB018531 dtsR1; AB020624 muri D-glutaniate racemase AB023377 tkt transketolase AB024708 gltB; gltD Glutamine 2-oxoglutarate aminotransferase and small subunits AB025424 acn AB027714 rep Replication protein AB027715 rep; aad Replication protein; aminoglycoside AF005242 argC AF005635 gInA Glutamine AF030405 hisF AF030520 argG Argininosuccinate AF03 1518 argF Omnithine AF036932 aroD) 3-dehydroquinate dehydratase AF038548 pyc Pyruvate carboxylase 2 (continued) AF038651I dciAE; apt; ret Dipeptide-binding protein; adenine Wehmeier, L. et at. "The role of the Corynebacterium glutamicum rel gene in phosphoribosyltransferase; GTP (p)ppGpp metabolism," Microbiology, 144:1853-1862 (1998) _________________pyrophosphokinase AF04 1436 argR Arginine repressor AF045998 impA Inositol monophosphate phosphatase AF048764 argH Argin inosuccinate AF049897 argC; argi; argB; N-acetylgtlutamnyl phosphate reductase; argD; argF; argR; ornithine acetyttransferase; NargG; argH acetytgtutamnate kinase; acetylornithine transminase; ornithine carbamoyltransferase; arginine repressor; argininosuccinate synthase; argininosuccinate lyase AF050 109 inbA Enoyl-acyt carrier protein reductase AF050 166 hisG ATP phosphoribosyltransferase 1846 hisA Phosphoribosytformii o-S-amimo-1-.

phosphoribosyt-4-im idazolecarboxamide isomerase AF052652 metA Homoserine 0-acetyltransferase Park, S. et al. "Isolation and analysis of metA, a methionine biosynthetic gene encoding homoserine acetylItransfe rase in Corynebacterium glutamicum," MoL Cells.. 8(3):286-294 (1998) AF053071I aroB Dehydroquinate synthetase AF060558 hisH Glutamine AF086704 hisE Phosphoribosyl-ATP- AFt 14233 aroA 5-enolpyruvytshikimate 3-phosphate A F 16184 panD L-aspartate-alpha-decarboxytlase precursor Dusch, N. et al. "Expression of the Corynebacterium glutamicum panD gene encoding L-aspartate-atpha-decarboxy lase leads to pantothenate overproduction in Escherichia coli," Appi. Environ. Microbial. 65(4)1t530- AF124518 aroD); aroE 3-dehydroquinase; shikimate -AF 124600 aroC; aroK; aroB; Chorismate synthase; shikimate kinase; 3pepQ dehydroquinate synthase; putative peptidase AF145897 inhA AF145898 inhA 2 (continued) AJ001436 ectP Transport of ectoine, glycine betaine, Peter, H. et "Corynebacterium glutaniicumn is equipped with four secondary proline carriers for compatible solutes: Identification, sequencing, and characterization of the proline/ectoine uptake system, ProP, and the ectoine/proline/glycine betaine carrier, EctP," J Bacterial.. 180(22):6005-6012 (1998) AJO04934 dapD Tetrahydrodipicolinate succinylase Wehrmann, A. et at. "Different modes of diaminopimelate synthesis and their (incomplete') role in cell wall integrity: A study with Corynebacterium glutamicum," J.

180(1 2):3 159-3165 (1998) AJ007732 ppc; secG; amt; ocd; Phosphoenolpyruvate-carboxylase; high soxA affinity ammonium uptake protein; putative omnithine-cyclodecarboxylase; sarcosine oxidase A.1010319 ftsY, glnB, gInD; srp; Involved in cell division; PH1 protein; Jakoby, M. et al. "Nitrogen regulation in Corynebacterium glutamicum; amtP uridylyltransferase (uridylyl-removing Isolation of genes involved in biochemical characterization of corresponding enzrnye); signal recognition particle; low proteins," FEMS Microbial., 173(2):303-310 (1999) amimonium uptake AJ132968 cat Chloramphenicol aceteyl transferase AJ224946 mqo L-malate: quinone oxidoreductase Molenaar, D. et al. "Biochemical and genetic characterization of the membrane-associated malate dehydrogenase (acceptor) from Corynebacterium Eur. J. Biochem., 254(2):395-403 (1998) AJ238250 ndh NADH dehydrogenase AJ238703 porA Porin Lichtinger, T. et al. "Biochemical and biophysical characterization of the cell wall porin of Corynebacterium glutamicum: The channel is formed by a low mass polypeptide," Biochemistry, 37(43):15024-15032 (1998) D17429 Transposable element IS31831 Vertes et al."lsolation and characterization of IS3183 1, a transposable eleme-t Corynebacterium glutamicum," Mol. Microbiol., 1 1(4):739-746 (1994) D84 102 odhA 2-oxoglutarate dehydrogenase Usuda, Y. et al. "Molecular cloning of the Corynebacterium glutamnicumn (Brevibacteriumn lactofermentumn A1 12036) odhA gene encoding a novel type 2-oxoglutarate dehydrogenase," Microbiology. 142:3347-3354 (1996) E01358 hdh; hk Homoserine dehydrogenase; homoserine Katsumata, R. et al. "Production of L-thereonine and L-isoleucine," Patent: JP 1987232392-A I 10/12/87 E01359 Upstream of the start codon of homoserine Katsumata, R. et al. "Production of L-thereonine and L-isoleucine," Patent: JP gene 1987232392-A 2 10/12/87 E01375 __________Tryptophan operon E01376 trpL; trpE Leader peptide; anthranilate synthase Matsui, K. et al. "Tryptophan operon, peptide and protein coded thereby, utilization of tryptophan operon gene expression and production of Patent: JP 1987244382-A I 10/24/87 2 (continued) E01377 Promoter and operator regions of Matsui, K. et "Tryptophan operon, peptide and protein coded thereby, tryptophan operon utilization of tryptophan operon gene expression and production of Patent: JP 1987244382-A 1 10/24/87 E03937 Biotin-synthase Ta-atakeyama, K. et al. "DNA fragment containing gene capable of coding synthetase and its utilization," Patent: JP 1992278088-A 1 10/02/92 E04040 Diamino pelargonic acid aminotransferase Kohama, K. et al. "Gene coding diaminopelargonic acid aminotransferase and desthiobiotin synthetase and its utilization," Patent: JP 1992330284-A I E04041 Desthiobiotinsynthetase Kohama, K. et al. "Gene coding diaminopelargonic acid aminotransferase and desthiobiotin synthetase and its utilization," Patent: JP 1992330284-A 1 E04307 Flavum aspartase Kurusu, Y. et al. "Gene DNA coding aspartase and utilization thereof," Patent: 1993030977-A 1 02/09/93 E04376 Isocitric acid lyase Katsumata, R- et al. "Gene manifestation controlling DNA," Patent: JP 3 03/09/93 E04377 Isocitric acid lyase N-terminal fragment Katsumata, R. et al. "Gene manifestation controlling DNA," Patent: JP 3 03/09/93 E04484 Prephenate dehydratase Sotouchi, N. et al. "Production of L-phenylalanine by fermentation," Patent: JP 1993076352-A 2 03/30/93 108 Aspartokinase Fugono, N. et al. "Gene DNA coding Aspartokinase and its use," Patent: JP 1 07/27/93 E0511 2 Dihydro-dipichorinate synthetase Hatakeyamna, K. et al. "Gene DNA coding dihydrodipicolinic acid synthetase its use," Patent: JP 199318437 1-A 1 07/27/93 E05776 Diaminopimelic acid dehydrogenase Kobayashi, M. et al. "Gene DNA coding Diaminopimelic acid dehydrogenase its use," Patent: JP 1993284970-A 1 11/02/93 E05779 Threonine synthase Kohama, K. et al. "Gene DNA coding threonine synthase and its use," Patent: JP 1993284972-A 1 I11/02/93 E061 10 Prephenate dehydratase Kikuchi, T. et al. "Production of L-phenylalanine by fermentation method," Patent: JP 199334488 I -A 1 12/27/93 E061 I I Mutated Prephenate dehydratase Kikuchi, T. et al. "Production of L-phenylalanine by fermentation method," Patent: JP 199334488 1-A 1 12/27/93 E06 146 Acetohydroxy acid synthetase lnui, M. et al. "Gene capable of coding Acetohydroxy acid synthetase and its use," Patent: JP 1993344893-A 1 12/27/93 E06825 Aspartokinase Sugimoto, M. et al. "Mutant aspartokinase gene," patent: JP 1994062866-A 1 E06826 Mutated aspartokinase alpha subunit Sugimoto, M. et al. "Mutant aspartokinase gene," patent: JP 1994062866-A I 03/08/94 2 continued) E06827 Mutated aspartokinase alpha subunit Sugimoto, M. et at. "Mutant aspartokinase gene," patent: JP 1 994062866-A 1 E07701 secY Honno, N. et al. "Gene DNA participating in integration of membraneous to membrane," Patent: JP 1994169780-A 1 06/21/94 E08 177 Aspartokinase Sato, Y. et al. "Genetic DNA capable of coding Aspartokinase released frm inhibition and its utilization," Patent: JP 1994261766-A 1 09/20/94 E08 178, Feedback inhibition-released Aspartokinase Sato, Y. et al. "Genetic DNA capable of coding Aspartokinase released from E08 179, feedback inhibition and its utilization," Patent: JP 1994261766-A 1 09/20/94 E08 180, E0818l1, E08 182 E08232 Acetohydroxy-acid isomeroreductase Inui, M. et al. "Gene DNA coding acetohydroxy acid isomeroreductase," JP 1994277067-A 1 10/04/94 E08234 secE Asai, Y. et at. "Gene DNA coding for translocation machinery of protein," JP 1994277073-A 1 10/04/94 E08643 FT aminotransferase and desthiobiotin Hatakeyama, K. et al. "DNA fragment having promoter function in synthetase promoter region corynefonn bacterium," Patent: JP 1995031476-A 1 02/03/95 E08646 Biotin synthetase Hatakeyama, K. et al. "DNA fragment having promoter function in bacterium," Patent: JP 1995031476-A 1 02/03/95 E08649 Aspartase Kohama, K. et al "DNA fragment having promoter function in coryneform bacterium," Patent: JP 199503 1478-A 1 02/03/95 E08900 Dihydrodipicolinate reductase Madori, M. et at. "DNA fragment containing gene coding Dihydrodipicolinate acid reductase and utilization thereof," Patent: JP 1995075578-A 1 03/20/95 E08901 Diaminopimetic acid decarboxylase Madori, M. et at. "DNA fragment containing gene coding Diaminopimelic acid decarboxylase and utilization thereof," Patent: JP 1995075579-A 1 03/20/95 E12594 Serine hydroxymethyltransferase Hatakeyama, K. et "Production of L-trypophan," Patent: JP 199702839 1I-A 1 02104/97 E12760, transposase Moriya, M. et at. "Amplification of gene using artificial transposon," Patent: E12759, JP 1997070291 -A 03/18/97 E 12764 Arginyl-tRNA synthetase; diaminopimelic Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: decarboxytase JP 1997070291 -A 03/18/97 E12767 Dihydrodipicolinic acid synthetase Moriya, M. et al. "Amplification of gene using artificial transposon," Patent: J P 1997070291 -A 03/18/97 E 12770 aspartokinase Moriya, M. et at. "Amplification of gene using artificial transposon," Patent: P197021-A 03/18/97 E 12773 Dihydrodipicolinic acid reductase Moriya, M. et at. "Amplification of gene using artificial transposon," Patent: J JP 1997070291 -A 03/18/97 Tal2co nud E13655 Glucose-6-phosphate dehydrogenase Hatakeyama, K. et al. "Glucose-6-phosphate dehydrogenase and DNA capable coding the same," Patent: J P 199722466 1-A 1 09/02/97 LO 1508. INvA Threonine dehydratase Moeckel, B. et al. "Functional and structural analysis of the threonine dehydratase of Corynebacterium glutamicum," J Bacteriol., 174:8065-8072 L07603 EC 4.2.1.15 3-deoxy-D-arabinoheptulosonate-7- Chen, C. et al. "The cloning and nucleotide sequence of Corynebacterium phosphate synthase glutamicum 3-deoxy- D-arab inoheptu losonate-7-phosph ate synthase gene," Microbial. Leti., 107:223-230 (1993) L09232 llvB; ilvN; ilvC Acetohydroxy acid synthase large subunit; Keilhauer, C. et al. "Isoleucine synthesis in Corynebacterium glutamicum: Acetohydroxy acid synthase small subunit; molecular analysis of the ilvB-ilvN-ilvC operon," J Bacterial., 175(17):5595acid isomeroreductase 5603 (1993) L18874 PtsM Phosphoenolpyruvate sugar Fouet, A et al. "Bacillus subtilis sucrose-specific enzyme 11 of the phosphotransferase phosphotransferase system: expression in Escherichia coli and homology to enzymes 11 from enteric bacteria," PNAS USA, 84(24):8773-8777 (1987); Lee, J.K. et al. "Nucleotide sequence of the gene encoding the Corynebacterium glutamicum mannose enzyme 11 and analyses of the deduced protein FEMS Microbial. Let., 1 19(1-2):137-145 (1994) L27 123 aceB Malate synthase Lee, H-S. et al. "Molecular characterization of aceB, a gene encoding malate synthase in Corynebacterium glutamicum," J Microbial. BiotechnoL., 4(4):256-263 (1994) L27126 yratknaeJetten, M. S. et al. "Structural and functional analysis of pyruvate kinase from Corynebacterium glutamicum," AppI. Environ. Microbial, 60(7):2501-2507 L28760 aceA Isocitrate lyase L35906 dtxr Diphtheria toxin repressor Oguiza, J.A. et al. "Molecular cloning, DNA sequence analysis, and characterization of the Corynebacterium diphtheriae dtxR from Brevibacterium lactofernientum," J Bacterial., 1 77(2):465-467 (1995) *M13 774 Prephenate dehydratase Follettie, M.T. et al. "Molecular cloning and nucleotide sequence of the ____________Corynebacterium glutamicum pheA gene,".J Bacterial., 167:695-702 (1986) *M16175 5S rRNA Park, Y-H. et al. "Phylogenetic analysis of -the coryneform bacteria by 56 sequences," J Baclerial, 169:1801-1806 (1987) 16663 trpE Anthranilate synthase, 5' end Sano, K. et al. "Structure and function of the trp operon control regions of Brevibacterium lactofernientum, a glu tam ic-acid-produci ng bacterium," Gene, (1987) M 16664 trpA Tryptophan synthase, 3Vend Sano, K. et al. "Structure and function of the trp operon control regions of Brevibacterium lactofermenturn, a glutam ic-acid-producing bacterium," Gene, (1987) (continued) M25819 Phosphoenolpyruvate carboxylase O'Regan, M. et al. '.Cloning and nucleotide sequence of the Phosphoenolpyruvate carboxylase-coding gene of Corynebacterium ATCC 13032," Gene, 77(2):23 7-251 (1989) 106 23S rRNA gene insertion sequence Roller, C. et al. "Gram-positive bacteria with a high DNA G+C content are characterized by a common insertion within their 23S rRNA genes," J Gen.

138:1167-1175 (1992) 107, 23S rRNA gene insertion sequence Roller, C. et al. "Gram-positive bacteria with a high DNA G+C content are 108 characterized by a common insertion within their 23S rRNA genes," J Gen.

icrobial, 138:1167-1175 (1992) M89931 aecD; brnQ; yhbw Beta C-S lyase; branched-chain amino acid, Rossol, 1. et al. "The Corynebacteriumn glutamicum aecD gene encodes a C-S uptake carrier; hypothetical protein yhbw lyase with alpha, beta-elimination activity that degrades aminoethylcysteine," J Bacleriol, 174(9):2968-2977 (1992); Tauch, A. et al. "Isoleucine uptake in Corynebacterium glutamicum ATCC 13032 is directed by the bmQ gene Arch. Microbiol. 169(4):303-3 12 (1998) S59299 trp Leader gene (promoter) Herry, D.M. et al. "Cloning of the trp gene cluster from a tryptophanhyperproducing strain of Corynebacterium glutamicum: identification of a mutation in the trp leader sequence," Appi. Em'iron. Microbial., 59(3):79 1-799 UI 1545 trpD) Anthranilate phosphoribosyltransferase O'Gara, J.P. and Dunican, L.K. (1994) Complete nucleotide sequence of the Corynebacterium glutamicum ATCC 21850 tpD gene." Thesis, Microbiology University College Galway, Ireland.

U 13922 cglIM; cgllR; clglIR Putative type 11 5-cytosoine Schafer, A. et al. "Cloning and characterization of a DNA region encoding a methyltransferase; putative type 11 stress-sensitive restriction system from Corynebacterium glutaniicumn ATCC restriction endonuclease; putative type I or .13032 and analysis of its role in intergeneric conjugation with Escherichia type Ill restriction endonuclease coli," J Bacteriol., 176(23):7309-7319 (1994); Schafer, A. et al. "The Corynebacterium glutamicum cglIM gene encoding a 5-cytosine in an McrBC- Escherichia coli strain," Gene, 203(2):95-101 (1997) U 14965 recA U31224 ppx Ankri, S. et al. "Mutations in the Corynebacterium glutamicumproline biosynthetic pathway: A natural bypass of the proA step," Ji Bacteriol., (1996) U31225 proC L-proline: NADP+ 5-oxidoreductase Ankri, S. et al. "Mutations in the Corynebacterium glutamicumproline biosynthetic pathway: A natural bypass of the proA step," J BacterioL., 178( 15):44 12-44 19 (1996) U3 1230 obg; proB; unkdh ?;gamma glutamyl kinase;similar to D- .Ankri, S. et al. "Mutations in the Corynebacterium glutamicumproline isomer specific 2-hydroxyacid biosynthetic pathway: A natural bypass of the proA step," J Bacteriol., 178(1 5):44 12-4419 (1996) Table 2 (continued) U31281 bioB Biotin synthase Serebriiskii, "Two new members of the bio Bsuperfamily: Cloning, sequencing and expression of bio B genes of Methylobacillus flagellatum and glutamicum," Gene, 175:15-22 (1996) U35023 thtR; accBC T'hiosulfate sulfurtransferase; acyl CoA Jager, W. et al. "A Corynebacterium glutamicum gene encoding a two-domain carboxylase protein similar to biotin carboxylases and biot in-carboxylI-carrier proteins," Microbiol., 1 66(2);76-82 (1996) U43535 cmr Multidrug resistance protein Jager, W. et al. "A Corynebacterium glutamicum gene conferring multidrug resistance in the heterologous host Escherichia coli," J Bacteriol., 79(7):2449-245 1 (1997) U43536 clpB Heat shock ATP-binding U53587 aphA-3 3'5' -aminoglycoside phosphotransferase U89648 Corynebacterium glutamicum unidentified sequence involved in histidine biosynthesis, sequence X04960 trpA; trpB; trpC; trpD; Tryptophan operon Matsui, K. et al. "Complete nucleotide and deduced amino acid sequences of trpE; trpG; trpL the Brevibacterium lactofermentumn tryptophan operon," Nucleic Acids Res., (1986) X07563 lys A DAP decarboxylase (meso-diaminopimelate Yeh, P. et al. "Nucleic sequence of the lysA gene of Corynebacterium decarboxylase, EC 4.1.1.20) glutamicum and possible mechanisms for modulation of its expression," Mo!.

Genet., 212(1):1 12-119 (1988) X 14234 EC 4.1.1.31 Phosphoenolpyruvate carboxylase Eikmanns, B.J. et al. "The Phosphoenolpyruvate carboxylase gene of Corynebacterium glutamicum: Molecular cloning, nucleotide sequence, and expression," Mo!. Gen. Genet., 218(2):330-339 (1989); Lepiniec, L. et al.

"Sorghum Phosphoenolpyruvate carboxylase gene family: structure, function and molecular evolution," Plan. Mo!. Biol., 21 (3):487-502 (1993) X1 73 13 fda Fructose-bisphosphate aldolase Von der Osten, C.H. et al. "Molecular cloning, nucleotide sequence and finestructural analysis of the Corynebacterium glutamnicum fda gene: structural comparison of C. glutamicum fructose-I, 6-biphosphate aldolase to class I and class 11 aldolases," Mo!. Microbiol., X53993 dapA L-2, 3-dihydrodipicolinate synthetase (EC Bonnassie, S. et al. "Nucleic sequence of the dapA gene from 4.2.1.52) Corynebacterium glutamicum," Nucleic Acids Res., 18(21):6421 (1990) X54223 AttB-related site Cianciotto, N. et al. "DNA sequence homology between att B3-related sites of Corynebacterium diphtheriae, Corynebacterium ulcerans, Corynebacterium glutamicum and the attP site of lambdacorynephage," FEMS. Microbiol, 66:299-302 (1990) X54740 argS; lysA Arginyl-tRNA synthetase; Diaminopimelate Marcel, T. et al. "Nucleotide sequence and organization of the upstream region decarboxylase of the Corynebacterium glutamicum lysA gene," Mol. Microbiol., 4(11):18 19- 1 1830(1990) Table 2 (continued) X55994 trpL; trpE Putative leader peptide; anthranilate Heery, D.M. et al. "Nucleotide sequence of the Corynebacterium glutamicum component I trpE gene," Nucleic Acids Res., 1 8(23):7 138 (1990) X56037 thrC Threonine synthase Han, K.S. et al. "The molecular structure of the Corynebacteriumn glutamicum synthase gene," Mo!. Microbiol., 4(10):1693-1702 (1990) X56075 attB-related site Attachment site Cianciotto, N. et al. "DNA sequence homology between att B3-related sites of Corynebacterium diphtheriae, Corynebacteriumn ulcerans, Corynebacteriumn glutamicumn and the attP site of lambdacorynephage," FEMS. Microbiol, 66:299-302 (1990) X57226 lysC-alpha; lysC-beta; Aspartokinase-aipha subunit; Kalinowski, J. et al. "Genetic and biochemical analysis of the Aspartokinase asd A spartok inase- beta subunit; aspartate beta from Corynebacteriumn glutamicum," Mo!. Microbiol., 5(5):l1197-1204 (1991); semialdehyde dehydrogenase Kalinowski, J. et al. "Aspartokinase genes lysC alpha and lysC beta overlap and are adjacent to the aspertate beta-semnialdehyde dehydrogenase gene asd in ___________Corynebacterium glutamicum," Mol. Gen. Genet., 224(3):317-324 (1990) X59403 gap;pgc; tpi G lyceral dehyde-3 -phosphate; Eikmanns, B.J. "Identification, sequence analysis, and expression of a phosphoglycerate kinase; triosephosphate Corynebacteriumn glutamicumn gene cluster encoding the three glycolytic isomerase enzymes glyceraldehyde-3 -ph osph ate dehydrogenase, 3-phosphoglycerate kinase, and triosephosphate isomeras," J Bacteriol., 174(19):6076-6086 ___(1992) X59404 gdh Glutamate detiydrogenase Bormann, E.R. et al. "Molecular analysis of the Corynebacterium glutamicum gdh gene encoding glutamate dehydrogenase," Mo!. Microbiol., 6(3):3 17-326 ___(1992) X603 12 lysI L-lysine permease Seep-Feldhaus, A.H. et al. "Molecular analysis of the Corynebacterium glutamnicum lysI gene involved in lysine uptake," Mo!. Microbial., 5(12):2995- X66078 cop I Psi protein Joliff, G. et al. "Cloning and nucleotide sequence of the cspl gene encoding PSI1, one of the two major secreted proteins of Corynebacteriumn glutamicum: The deduced N-terminal region of PS I is similar to the Mycobacterium antigen complex," Mo!. Microbial., 6(16):2349-2362 (1992) X661 12 glt Citrate synthase Eikmanns, B.J. et al. "Cloning sequence, expression and transcriptional analysis of the Corynebacteriumn glutamicum gltA gene encoding citrate Microbial., 140:1817-1828 (1994) X6773 7 dapB Dihydrodipicolinate X69 103 csp2 Surface layer protein PS2 Peyret, J.L. et al. "Characterization of the cspB gene encoding PS2, an ordered surface-layer protein in Corynebacterium glutamicum," Mot. Microbial..

(1993) X69 104 1S3 related insertion element Bonamy, C. et al. "Identification of IS 1206, a Corynebacteriumn glutamicumn IS3-related insertion sequence and phylogenetic analysis," Mdo!. Microbial., 14(3):571-581 (1994) 2 (continued) X70959 leuA Isopropylmalate synthase Patek, M. et al. "Leucine synthesis in Corynebacteriumn glutamicum: enzyme activities, structure of leuA, and effect of IeuA inactivation on lysine X7149 ic Isoitrae dhydrgenae ~AP+)synthesis," App!. Environ. Microbio., 60(1): 133-140 (1994) X7149 ic Isoitrae deydroenas (NAP+)Eikmanns, B.J. et al. "Cloning sequence analysis, expression, and inactivaion 0 of the Corynebacteriumn glutamicum icd gene encoding isocitrate dehydrogenase and biochemical characterization of the enzyme," J. Bacteriol., (1995) X72855 GDHA Glutamate dehydrogenase (NADP+) X75083, mtrA 5-methyltryptophan resistance Heery, D.M. et al. "A sequence from a tryptophan-hyperproducing strain of X70584 Corynebacterium glutamicumn encoding resistance to Biophys. Res. Commun., 201(3):1255-1262 (1994) X75085 recA Fitzpatrick, R. et al. "Construction and characterization of recA mutant strains of Corynebacteriumn glutamicum and Brevibacteriumn lactofernientum," App!.

Biltechno!., 42(4):575-580 (1994) X75504 aceA; thiX Partial Isocitrate lyase; Reinscheid, D.J. et al. "Characterization of the isocitrate lyase gene from Corynebacteriumn glutamicum and biochemical analysis of the enzyme," J 176(1 2):3474-3483 (1994) X76875 ATPase beta-subunit Ludwig, W. et al. "Phylogenetic relationships of bacteria based on comparative sequence analysis of elongation factor Tu and ATP-synthase beta-subunit Van Leeu-wenhoek, 64: 285-305 (1993) 0 X77034 tuf Elongation factor Tu Ludwig, W. et al. "Phylogenetic relationships of bacteria based on comparative' sequence analysis of elongation factor Tu and ATP-synthase beta-subunit Anionie Van Leeuwenhoek~ 64:285-305 (1993) X77384 recA Billman-Jacobe, "Nucleotide sequence of a recA gene from Corynebacterium glutamicum," DNA Seq., 4(6):403-404 (1994) X78491I aceB Malate synthase Reinscheid, D.J. et al. "Malate synthase from Corynebacteriumn glutamicumn pta-ack operon encoding phosphotransacetylase: sequence analysis," 140:3099-3108 (1994) X80629 16S rDNA 16S ribosomal RNA Rainey, F.A. et al. "Phylogenetic analysis of the genera Rhodococcus and Norcardia and evidence for the evolutionary origin of the genus Norcardia from within the radiation of Rhodococcus species," MicrobioL, 141:523-528 (1995) X81 191' gluA; gluB; gluC; Glutamate uptake system Kronemeyer, W. et al. "Structure of the gluA BCD cluster encoding the gluD glutamate uptake system of Corynebacteriumn glutamicum," J. Bacteriol., 177(5):1 152-1158 (1995) X81379 dapE Succinyldiaminopimelate desuccinylase Wehrmann, A. et al. "Analysis of different DNA fragments of Corynebacteriumn glutamicumn complementing dapE of Escherichia coli," 40:3349-56 (1994) 2 (continued) X82061 16S rDNA 16S ribosomal RNA Ruimy, R. et al. "Phylogeny of the genus Corynebacterium deduced from analyses of small-subunit ribosomal DNA sequences," Int. J Syst. Bacterial., (1995) X82928 asd; IysC Aspartate-semialdehyde dehydrogenase; Serebrijski, 1. et al. "Multicopy suppression by asd gene and osmotic stressdependent complementation by heterologous proA in proA mutants," J Bacterial, 1 77(24):7255-7260 (1995) X82929 proA Garnma-glutamyl phosphate reductase Serebrijski, 1. et al. "Multicopy suppression by asd gene and osmotic stressdependent complementation by heterologous proA in proA mutants," J.

1 77(24):7255-7260 (1995) X84257 16S rDNA 16S ribosomal RNA Pascual, C. et al. "Phylogenetic analysis of the genus Corynebacterium based on 16S rRNA gene sequences," Int. J Syst. Bacteriol., 45(4):724-728 (1995) X85965 aroP; dapE Aromatic amino acid permease; Wehrmann et al. "Functional analysis of sequences adjacent to dapE of C.

glutamicum proline reveals the presence of aroP, which encodes the aromatic acid transporter," J Bacterial., 177(20):5991-5993 (1995) X86157 argB; argC; argD; Acetylgiutamnate kinase; N-acetyl-gamma- Sakanyan, V. et al. "Genes and enzymes of the acetyl cycle of arginine argF; argi gl utamylI-phosphate reductase; biosynthesis in Corynebacterium glutamicum: enzyme evolution in the early acetylornithine aminotransferase; omnithine steps of the arginine pathway," Microbiology, 142:99-108 (1996) carbamoyltransferase; glutamate Nacetyltransferase X89084 pta; ackA Phosphate acetyltransferase; acetate kinase Reinischeid, D.J. et al. "Cloning, sequence analysis, expression and inactivation of the Corynebacterium glutamicum pta-ack operon encoding phosphotransacety lase and acetate kinase," Microbiology, 145:503-513 (1999) X89850 attB Attachment site Le Marrec, C. et al. "Genetic characterization of site-specific integration functions of phi AAU2 infecting "Arthrobacter aureus C70," J Bacterial., 1996-2004 (1996) X90356 Promoter fragment FlI Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbilgy, 142:1297-1309 (1996) X90357 Promoter fragment F2 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis ind search for a consensus motif," Microbiology, (1996) X90358 Promoter fragment FI10 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90359 Promoter fragment F 13 Patek, M et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Mi craobiology (1996) 2 (continued) X90360 Promoter fragment P22 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90361 Promoter fragment F34 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90362 Promoter fragment F37 Patek, M. et al. "Promoters from C. glutamicum: cloning, molecular analysis search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90363 Promoter fragment F45 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, (1996) X90364 Promoter fragment F64 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90365 Promoter fragment. P75 Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology, X9036 Pomotr fagmet PIOI142:1297-1309 (1996) X9036 Prmote frgmen PF 0 1Patek, M. et al. "Promoters from Corynebacterium glutamicum: cloning, molecular analysis and search for a consensus motif," Microbiology 142:1297-1309 (1996) X90367 Promoter fragment PPF104 Patek, M. et al. "Promoters from Corynebacterium glutaniicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X90368 Promoter fragment PPF109 Patek, M. et al. "Promoters from Corynebacterium glutarnicum: cloning, molecular analysis and search for a consensus motif," Microbiology, 142:1297-1309 (1996) X93513 amt Ammonium transport system Siewe, R.M. et al. "Functional and genetic characterization of the (methyl) ammonium uptake carrier of Corynebacterium glutamicum," J Biol. Chem., 271l(10):5398-5403 (1996) X93514 betP Glycine betaine transport system Peter, H. et al. "isolation, characterization, and expression of the Corynebacterium glutamicum betP gene, encoding the transport system for the solute glycine betaine," J Bacieriol., 178(1 7):5229-5234 (1996) X95649 orf4 Patek, M. et al. "Identification and transcriptional analysis of the dapB-QRF2 dapA-ORF4 operon of Corynebacterium glutamicum, encoding two enzymes involved in L-lysine synthesis," Bioiechnol. Let., 19:1113-1117 (1997) X96471 lysE; lysG Lysine exporter protein; Lysine export Vrljic, M. et al. "A new type of transporter with a new type of cellular regulator protein function: L-lysine export from Corynebacteriumn glutamicum," Mot.

Microbiol., 22(5):815-826 (1996) Table 2 (continued) X96580 panB; panC; xylB 3-methyl-2-oxobutanoate Sahm, H. et al. "D-pantothenate synthesis in Corynebacterium glutamicum and hydroxymethyltransferase; pantoate-beta- use of panBC and genes encoding L-valine synthesis for D-pantothenate alanine ligase; xylulokinase overproduction," Appi. Environ. Microbiol., 65(5):1973-1979 (1999) X96962 sequence IS 1207 and transposase X99289 Elongation factor P Ramos, A. et al. "Cloning, sequencing and expression of the gene encoding elongation factor P in the amino-acid producer Brevibacterium lactofermentum glutamicum ATCC 13869)," Gene, 198:217-222 (1997) YGO 140 thrB Homoserine kinase Mateos, L.M. et al. "Nucleotide sequence of the homoserine kinase (thrB) gene the Brevibacterium lactofermentum," Nucleic Acids Res., 15(9):3922 (1987) Y00151 ddh Meso-diaminopimelate D-dehydrogenase Ishino, S. et at. "Nucleotide sequence of the meso-d iam inopi me late D- (EC 1.4.1 .16) dehydrogenase gene from Corynebacterium glutamicum," Nucleic Acids Res., (1987) Y00476 thrA Homoserine dehydrogenase Mateos, L.M. et al. "Nucleotide sequence of the homoserine dehydrogenase (thrA) gene of the Brevibacterium lactofermentum," Nucleic Acids Res., 15(24): 10598 (1987) Y00546 horn; thrB Homoserine dehydrogenase; homoserine Peoples, O.P. et al. "Nucleotide sequence and fine structural analysis of the kinase Corynebacterium glutam icum hom-thrB operon," Mol. Microbial.. 2(1 ):63-72 Y08964 murC; ftsQ/divD; ftsZ UPD-N-acetylmuramate-alanine ligase; Honrubia, M.P. et al. "Identification, characterization, and chromosomal division initiation protein or cell division organization of the ftsZ gene from Brevibacterium Iactoferrnentum," M6. Gen.

cell division protein Genet., 259(1):97-104 (1998) Y09 163 putP High affinity proline transport system Peter, H. et al. "Isolation of the putP gene of Corynebacterium glutamicumproline and characterization of a low-affinity uptake system for solutes," Arch. Microbial., 168(2):143-151 (1997) Y09548 pyc Pyruvate carboxylase Peters-Wendisch, P.G. et al. "Pyruvate carboxylase from Corynebacterium glutamicum: characterization, expression and inactivation of the pyc gene," ___________Microbiology, 144:915-927 (1998) Y09578 leuB 3-isopropylmalate dehydrogenase Patek, M. et at. "Analysis of the leuB gene from Corynebacterium App. Microbial. Biotechnol., 50(l):42-47 (1998) Y 12472 Attachment site bacteriophage Phi- 16 Moreau, S. et al. "Site-specific integration of corynephage Phi- 16: The construction of an integration vector," Microbial., 145:539-548 (1999) Y 12537 proP Proline/ectoine uptake system protein Peter, H. et al. "Corynebacterium glutamicum is equipped with four secondary carriers for compatible solutes: Identification, sequencing, and characterization of the prolinefectoine uptake system, ProP, and the ectoine/proline/lglycine carrier, EctP," J Bacteriol., 180(22):6005-6012 (1998) 2 continued) Y 13221 ginA Glutamine synthetase I Jakoby, M. et al. "Isolation of Corynebacterium glutamicum gInA gene glutam ine synthetase FEMS Microbiol Let., 154(1 1-88 (1997) Y 16642 lpd Dihydrolipoamide dehydrogenase Y 18059 Attachment site Corynephage 304L Moreau, S. et al. "Analysis of the integration functions of φ304L: An module among corynephages," Virology, 255(1):150-159 (1999) Z21501 argS; lysA AXrginy-tRNA synthetase; diaminopimelate Oguiza, J.A. et al. "A gene encoding arginyl-tRNA synthetase is located in the decarboxylase (partial) upstream region of the lysA gene in Brevibacterium lactofermentum: Regulation of argS-IysA cluster expression by arginine," J.

I175(22):73 56-7362 (1993) Z21502 dapA; dapB Dihydrodipicolinate synthase; Pisabarro, A. et al. "A cluster of three genes (dapA, orf2, anid dapB) of dihydrodipicolinate reductase Brevibacterium lactofermentumn encodes dihydrodipicolinate reductase, and a third polypeptide of unknown function," J. Bacteriol., 1 75(9):2743-2749 Z29563 thrC Threonine synthase Malumbres, M. et al. "Analysis and expression of the thrC gene of the encoded Z46753_____ 16S____eneor_1SriosomlRN threonine synthase," Appi. Environ. Microbiol., 60(7)2209-2219 (1994) Z49822 sigA SigA sigma factor Oguiza, J.A. et al "Multiple sigma factor genes in Brevibacterium lactofermentum: Characterization of sigA and sigB," J. Bacteriol., 178(2):550- (1996) Z49823 galE; dtxR Catalytic activity UDP-galactose 4- Oguiza, J.A. et al "The galE gene encoding the UDP-galactose 4-epimerase of epimerase; diphtheria toxin regulatory Brevibacteriumn lactofermentum is coupled transcriptionally to the dmdR gene," Gene, 177:103-107 (1996) Z49824 orfl; sigfl SigB sigma factor Oguiza, J.A. et al "Multiple sigma factor genes in Brevibacterium lactofermentum: Characterization of sigA and sigB," J. Bacteriol., 178(2):550- 53 (996) Z66534 Transposase Correia, A. et al. "Cloning and characterization of an IS-like element present in the genome of Brevibacterium lactofermentum ATCC 13869," Gene, (1996) 'A sequence for this gene was published in the indicated reference. However, the sequence obtained by the inventors of the present application is significantly longer than the published version. It is believed that the published version relied on an incorrect start codon, and thus represents only a fragment of the actual coding region.

WO 01/00843 WO 0100843PCTIBOO/00923 -91- TABLE 3: Corynebacterium and Brevibacterium Strains Which May be Used in the Practice of the Invention .fifl f- "gV fERM NR1U GE wFCim wS?~ iCT Brevibacterium ammoniagenes 21054 Brevibacterium ammoniagenes 19350 Brevibacterium ammoniagenes 19351 Brevibacterium ammoniagenes 19352 Brevibacterium ammoniagenes 19353 Brevibacterium ammoniagenes 19354 Brevibactenium ammoniagenes 19355 Brevibacterium ammoniagenes 19356 Brevibacterium ammoniagenes 21055 Brevibacterium ammoniagenes 21077 Brevibacterium ammoniagenes 21553 Brevibacterium ammoniagenes 21580 Brevibacterium ammoniagenes 39101 Brevibacterium butanicum 21196 Brevibacterium divaricatum 21792 P928 Brevibacterium flavum 21474 Brevibacterium flavum 21129 Brevibacterium flavum 21518 Brevibacterium flavum BI 1474 Brevibacterium flavum 1472 Brevibacterium flavum 21127 Brevibacterium flavum 21128 Brevibacterium flavum 21427 Brevibacterium flavum 21475 Brevibacterium flavum 21517 Brevibacterium fiavum 21528 Brevibacterium fiavum Brevibacterium flavum BI 11477 Brevibacterium flavum BI 11478 Brevibacterium fiavum 21127 Brevibacterium flavum 1474 Brevibacterium healii 15527 Brevibacterium ketoglutamicum 21004 Brevibacterium ketoglutamicum 21089 Brevibacterium ketosoreductum 21914 Brevibacterium lactofermentum Brevibacterium lactofermentum 74 Brevibacterium lactofermentum 77 Brevibacterium lactofermentum 21798 Brevibacterium lactofermentum 21799 Brevibactenium lactafermentum 21800 Brevibacterium lactofermnentum 21801 Brevibacterium lactofermentum BI 11470 B7vbctru Iactofermentum B 11471 WO 01/00843 WO 0100843PCTJIBOO/00923 92 Brevibacterium lactoferinentum 21086 Brevibacterium lactofermentumn 21420 Brevibacterium lactofermentumn 21086 Brevibacterium lactofermnentumn 31269 Brevibacteriuni linens 9174 Brevibacterium linens 19391 Brevibacterium linens 8377 Brevibacterium paraffinolyticum 11160 Brevibacterium Spec. Brevibacterium Spec. ____717.73 Brevibacterium Spec. 14604 Brevibacterium Spec. 21860 Brevibacterium Spec. 21864 Brevibacterium Spec. 21865 Brevibacterium Spec. 21866 Brevibacterium Spec. 19240 Corynebacterium acetoacidophilumn Corynebacterium acetoacidophilumn 13870 Corynebacterium acetoglutamnicumn BI 1473 Corynebacterium acetoglutamnicumn B311475 Corynebacterium acetoglutamicum 15806 Corynebacterium acetoglutamicum 21491 Corynebacterium acetoglutamnicumn 31270 Corynebacterium acetophilumn B3671 Corynebacterium ammoniagenes 6872 2399 Corynebacterium ammoniagenes 15511 Corynebacterium fujiokense 21496 Corynebacterium glutamnicumn 14067 Corynebacterium glutamnicumn 39137 Corynebacterium glutamnicumn 21254 Corynebacterium glutamnicumn 21255 Corynebacterium glutamnicum 31830 Corynebacterium glutamnicumn 13032 Corynebactenium glutamnicumn 14305 Corynebacterium glutamnicumn 15455 Corynebacterium glutarnicumn 13058 Corynebacterium glutamicum 13059 Corynebacterium glutamnicum 13060 Corynebacterium glutamnicumn 21492 Corynebacterium glutamnicumn 21513 Corynebacterium glutamnicumn 21526 Corynebacterium glutamnicumn 21543 Corynebacterium glutamnicumn 13287 Corynebacterium glutarnicumn 21851 Corynebacterium glutarnicumn 21253 Corynebacterium glutamnicumn 21514 Corynebacterium glutamicum 21516 Corynebacterium glutamnicumn 21299 WO 01/00843 WO 0100843PCTI/iBOO/00923 93 icii' ERM CN~1 CE.0T KGM NCS ~DM Corynebacterium glutamicum 21300 Corynebacterium glutamicum 2164 Corynebacterium glutamicum 21650 Corynebacterium glutamicum 219 Corynebacterium glutamicum 213690 Corynebacterium glutamicum 121723__ Corynebacterium glutamicum 118 Corynebacterium glutamicum 211597______ Corynebacterium glutamicum 213558_______ Corynebacterium glutamicum 3180159_____ Carynebacterium glutamicum Corynebacterium glutamicum 2156808__ Corynebacterium glutamicum 21634 Corynebacterium glutamicum 21562 Corynebacterium glutamicum 215653___ Carynebacterium glutamicum 215664__ Corynebacterium glutamicum 215675___ Corynebacterium glutamicum 215686___ Corynebacterium glutarnicum Corynebacterium glutamicum 21570 Corynebacterium glutamicum 21571 Corynebacterium glutamicum 21572 Corynebacterium glutamicum 215731__ Corynebacterium glutaniicum 215792______ Corynebacterium glutamicum 21909573_ Corynebacterium glutamicum 21590 Corynebacterium glutamicum 1905149_____ Corynebacterium glutamnicum 190520___ Corynebacterium glutamicum 190531__ Corynebacterium glutaznicum 19054 Corynebacterium glutamicum 190553_______ Corynebacterium glutainicum 190564______ Corynebacterium glutamicum 19057 Corynebacterium glutamicum 190586______ Corynebacterium glutamicum 19057 Corynebacterium glutamicum 1906058_ Corynebacterium glutamicum 19159___ Corynebacterium glutamicum Corynebacterium glutamicum 1185 Corynebacterium glutamlc-um Corynebacterium glutamicum 2154415________ Corynebacterium glutamicum 2142___ Corynebacterium glutamicum 21544 883 Corynebacterium glutamicum B8182 Corynebacterium glutamicum Corynebacterium iglutamicum WO 01100843 WO 0100843PCT/IBOO/00923 94 Corynebacterium glutamicum B 12418 Corynebacterium glutamicum Bi 1476 Corynebacterium glutamicum 21608 Corynebacterium lilium P973 Corynebacterium nitrilophilus 21419 11594 Corynebacterium spec. P4445 Corynebacterium spec. P4446 Corynebacterium spec. 31088 Corynebacterium spec. 31089 Corynebacterium spec. 31090 Corynebacterium spec. 31090 Corynebacterium spec. 31090 Corynebacterium spec. 15954 20145 Corynebacterium spec. 21857 Corynebacterium spec. 21862 Corynebacterium spec. 21863 ATCC: American Type Culture Collection, Rockville, MD, USA FERM: Fermentation Research Institute, Chiba, Japan NRRL: ARS Culture Collection, Northern Regional Research Laboratory, Peoria, IL, USA CECT: Coleccion Espanola de Cultivos Tipo, Valencia, Spain NCIMB: National Collection of Industrial and Marine Bacteria Ltd., Aberdeen, UK CBS: Centraalbureau voor Schimmelcultures, Baamn, NL NCTC: National Collection of Type Cultures, London, UK DSMZ: Deutsche Sammiung von Mikroorganismen und Zelikulturen, Braunschweig, Germany For reference see Sugawara, H. et (1993) World directory of collections of cultures of microorganisms: Bacteria, fungi and yeasts (4h~ edn), World federation for culture collections world data center on microorganisms, Saimata, Japen.

Table 4: Alignment Results Length Accession Name of Genbank Hit ID length Genbank Hit

(NT)

Source of Genbank HIt homology

(GAP)

Date of 0 00 29-Jun-e 29-Jun-99 rxa00023 3579 GB..EST33:A1776129 483 GB-EST33:A1776129 483 A1776129 EST257217 tomato resistant, Cornell Lycopersicon esculentum cDNA clone cLERi 703, mRNA sequence.

A1776129 EST257217 tomato resistant, Cornell Lycopersicon esculentumn cONA clone cLERi 703, mRNA sequence.

Lycopersicon esculentumn 40,956 Lycopersicon esculentumn 40,956 rxa00044 1059 EM-PAT: E 1760 GBkyAT:126124 6911 Eli 1760 Base sequence of sucrase gene.

6911 126124 Sequence 4 from patent US 5556776.

Corynebacterium glutamicum GB-BA2:ECOUW89 176195 U00006 E. coli chromiosomal region from 89.2 to 92.8 minutes.

rxaOO064 1401 GBYAT:E16763 2517 E16763 gDNA encoding aspartate transferase (AAT).

Unknown.

Escherichia coi Corynebacterium glutamicum Drosophila melanogaster Drosophila melanogaster 42,979 08-OCT- 1997 (Rel.

52, Created) 42,979 07-OCT- 1996 39,097 1 7-DEC- 1993 95,429 28-Jul-99 GBHTG2:AC007892 13.4257 AC007892 GB-HTG2:AC007892 134257 AC007892 Drosophila melanogaster chromosome 3 clone BACR02003 (0797) RPCI-98 02.0.3 map 99B-99B strain y; cn bw sp, SEQUENCING IN PROGRESS 113 unordered pieces.

Drosophila melanogaster chromosome 3 clone BACRO2003 (0797) RPCI-98 02.0.3 map 9913-99B strain y; cn bw sp, SEQUENCING IN PROGRESS-, 113 unordered pieces.

31,111 31.111 2-Aug-99 2-Aug-99 rxaOO072 rxaOOlOS 798 GB-BA:M1VOO2 GB..BA1:ECU29581 GB BA2:AE000366 rxaOOlO6 579 GBEST15:AA494237 GB..BA2.AF161327 56414 AL008967 Mycobacterium tuberculosis H37Rv complete genome; segment 122/1 62.

71128 U29581 Escherichia col K-12 genome; approximately 63 to 64 minutes.

10405 AE000366 Eschenichia coli K-12 MG1655 section 256 of 400 of the complete genome.

367 AA494237 ng83fD4.sl NCICGAP..Pr6 Homo sapiens cDNA clone IMAGE:941407 similar to SW:DYRLACCA P00381 DIHYDROFOLATE REDUCTASE;, mRNA sequence.

2021 AF161327 Corynebactenium diphtherlae hlstldine klnase ChrS (chrS) and response regulator ChrA (chrA) genes, complete cds.

654 ARO4 189 Sequence 4 from patent US 5811286.

148336 AC007I1O Homo sapiens chromosome 17, clone hRPK.472-J 18, complete sequence.

Mycobacterium tuberculosis Escherichia coli Escherichia coli Homo sapiens Corynebacterium diphtheriae Unknown.

Homo sapiens 37,753 17-Jun-98 35,669 35,669 42,896 14-Jan-97 12-Nov-98 20-Aug-97 GBPATARO41 189 GB..PR4.AC0071 10 40,210 9-Sep-99 41,176 29-e-99 36,783 1999 t.j 40,296 2-Sep-99 W 1170 GBHTG3:AC008537 170030 AC008537 GBHTG3:AC008537 170030 AC008537 Homo sapiens chromosome 19 clone CIT-HSPC 490E21, SEQUENCING Homo sapiens IN PROGRESS 93 unordered pieces.

Homo sapiens chromosome 19 clone CIT-HSPC 490E21, SEQUENCING Homo sapiens IN PROGRESS 93 unordered pieces.

40,296 2-Sep-99 rxaOOl 16 1284 GB-BA2:AF062345 rxa00131 732 rxaOOl 32 1557 1059 rxaOOl46 1464 rxaOO147 1302 GB-PAT:I118647 GB-GSS1 3:AQ4461 9 7 GB-BA1 :MTY2OB1 1 GB BA1 :5AR7932 GB-BA1 :MTY2OB1 1 GB-BA1 :MTY2OB1 1 GB-N2:TVU40872 GB-HTG6:AC01 0706 GB-BA1 :MTCY2B12 GBBA1:PSEPYRBX GBBA1:LLPYRBDNA GB-BA1 :MTCY2B12 GB-BA1 :MTCY154 GBBA1:MSGY1S4 GB-BA1 :MTCV2B12 GBBA1:MSGB937C

S

GB-BA1 :PAU81 259 Table 4 (continued) 16458 AF062345 Caulobacter crescentus Sstl (sstl). S-layer protein subunit (rsaA), ABC Caulobacter cresoentus transporter (rsaD), membrane forming unit (rsaE), putative GDP-mannose-4,6dehydratase (IpsA), putative acetyltransferase (lpsB), putative perosamine synthetase (IpsC), putative mannosyltransferase (lpsD), putative mannosyltransferase (lpsE), outer membrane protein (rsaF), and putative perosamine transferase (IpsE) genes, complete cds.

3300 118647 Sequence 6 from patent US 5500353. Unknown.

751 AQ446197 nbxbOO62Dl 6r CUIGI Rice BAC Library Oryza sativa genomic clone Oryza sativa nbxbOO62Dl 6r, genomic survey sequence.

36330 Z95121 Mycobacterium tuberculosis H37Rv complete genome, segment 139/1 62. Mycobacterium tuberculosis 15176 AJ007932 Streptomyces argillaceus mithramycin biosynthetic genes. Streptomyces argillaceus 36330 Z95121 Mycobacteriumn tuberculosis H37Rv complete genome; segment 139/162. Mycobactedrlu tuberculosis 36330 Z95121 Mycobactedrn tuberculosis H37Rv complete genome; segment 139/1 62. Mycobacterium tuberculosis 1882 U40872 Trichomonas vaginalis S-adenosyl-L-homocysteine hydrolase gene, complete Trictiomonas vaginalis cds.

169265 AC010706 Drosophila melanogaster chromosome X clone BACR36D1 5 (03887) RPCI-98 Drosophila melanogaster 36.D.15 map 13C-13E strain y: cn bw sp, SEQUENCING IN PROGRESS 74 unordered pieces.

20431 Z8101 1 Mycobacterium tuberculosis H37Rv complete genome; segment 61/162. Mycobacterium tuberculosis 2273 Li19649 Pseudomonas aeruginosa aspartate transcarbamnoylase (pyrB) and Pseudomonas aeruginos dihydroorotase-like (pyrX) genes, complete cds's.

1468 X84262 L.leichmannil pyrB gene. Lactobacillus leichmannil 20431 Z81 011 Mycobacteriumn tuberculosis H37Rv complete genome; segment 61/162. Mycobacteriumn tuberculosis 13935 Z98209 Mycobacterium tuberculosis H37Rv complete genome; segment 121/162. Mycobacterium tuberculosis 40221 AD000002 Mycobacteriumn tuberculosis sequence from clone y154. Mycobacterium tuberculosis 20431 Z810111 Mycobacterium tuberculosis H37Rv complete genome; segment 61/1 62. Mycobacterium tuberculosis 38914 L78820 Mycobacterlum leprae cosmid B937 DNA sequence. Mycobacterium leprae 7285 U81259 Pseudomonas aeruginosa dihydrodipicolinate reductase (dapB) gene, partial Pseudomonas aeruginos cds, carbamnoylphosphate synthetase small subunit (carA) and carbamnoylphosphate synthetase large subunit (car8) genes, complete cds, and FtsJ homolog (ftsJ) gene, partial cds.

33320 AL009204 Streptomyces coelicolor cosmid 9B310. Streptomyces coelicolor a a 36,235 36,821 38,124 43,571 41,116 39,726 36,788 61,914 51,325 63,365 56,080 47,514 60,714 39,229 36,618 61,527 59,538 55.396 1999 00 07-OCT- 1996 8-Apr-99 1 7-Jun-98 15-Jun-99 17-Jun-98 1 7-Jun-98 31-OCT- 1996 22-Nov-990 1 8-Jun-98 26-Jul-93 29-Apr-97 1 8-Jun-98 17-Jun-98 03-DEC- 1996 18-Jun-98 115-Jun-96 23-DEC- 1996 rxaOO156 1233 GB-BA1:SC9B10 52,666 10-Feb-99 GB BA2:AF0021 33 15437 AF002133 rxa00166 783 rxaOOl98 672 rxaOO216 1113 rxaOO219 1065 rxa00223 1212 rxa00229 803 rxa00241 1626 GB-BA1:D85417 GBJ'1TG3AC008167 GB-HTG3ACO081 67 GB-HTG4:ACO1 0118 GB-BA1 -AB024708 GB-BAI :AB024708 GB-EST24:A1232702 GB_-ITG2:HSDJ85OE 9 GBHTG2:HSDJ850E 9 GB-PR2:CNSO1 OSA GB HTG2:AC005079 -0 GByHTG2AC005079 GBJ{TG2AC005079 -1 GBBA1:PPEA3NIF GB-BA2:AF128444 GB-HTG4AC0101 11 GBBA2:AF124518 GB!R3:AC004593 GBYTrG2:AC006907 GBBA1:CGLYSI 7984 174223 174223 80605 8734 8734 528 117353 117353 159400 110000 110000 110000 1977 1 2477 138938 1758 150221 188972 4232 D85417 AC0O81 67 AC0081 67 AC010118 AB024708 AB024708 A1232702 AL121758 AL121758 AL1 21766 AC005079 AC005079 AC005079 X99694 AF128444 AC010111 AF124518 AC004593 AC006907 X60312 Table 4 (continued) Mycobacterium avium strain GIR10 transcriptional regulator (may81) gene, Mycobacterium avium partial cds, aconitase (acn), invasin 1 (invi), Invasln 2 (Inv2), transcriptional regulator (moxR), ketoacyl-reductase (fabG). enoyl-reductase (inhA) and ferrochelatase (mav272) genes, complete cds.

Propionibacterium freudenreichii hemY, hemH, hemB, hemX, hemR and Propionibacterium hemL- genes, complete eds. freudenreichii Homo sapiens clone NH1 17201 3, SEQUENCING IN PROGRESS Homo sapiens unordered pieces.

Homo sapiens clone NI-01 7201 3, SEQUENCING IN PROGRESS ,7 Homo sapiens unordered pieces.

Drosophila melanogaster chromosome 31-162131 clone RPCI98-1001 5, Drosophila melanogaster SEQUENCING IN PROGRESS 51 unordered pieces.

Corynebacterium glutamlcum gRtB and gltD genes for glutamine 2- Corynebacterium oxoglutarate aminotransferase large and small subunits, complete cds. glutamicum Corynebacterlum glutamicum gRtB and gltD genes for glutamine 2- Corynebacterium oxoglutarate aminotransferase large and small subunits, complete cds. glutamicum EST229390 Normalized rat kidney, Bento Soares Rattus sp. cIJNA clone Rattus sp.

3Tend, mRNA sequence.

Homo sapiens chromosome 20 clone RP5-850E9, SEQUENCING IN Homo sapiens PROGRESS in unordered pieces.

Homo sapiens chromosome 20 clone RPS-850E9. SEQUENCING IN Homo sapiens PROGRESS In unordered pieces.

Human chromosome 14 DNA sequence -IN PROGRESS BAC R-4121-8 Homo sapiens of RPCI-1 1 library from chromosome 14 of Homo sapiens (Human), complete sequence.

Homo sapiens clone RG252P22,~ SEQUENCING IN PROGRESS*.*, 3 Homo sapiens unordered pieces.

Homo sapiens clone RG252P22, SEQUENCING IN PROGRESS Homo sapiens unordered pieces.

Homo sapiens clone RG252P22, SEQUENCING IN PROGRESS Homo sapiens unordered pieces.

Plasmid pEA3 nitrogen fixation genes. Enterobacter agglomerans Rhodobacter capsulatus molybdenum cofactor biosynthetic gene cluster, Rhodobacter capsulatus partial sequence.

Drosophla melanogaster chromosome 3L70C1 clone RPCI98-9B18, Drosophila melanogaster SEQUENCING IN PROGRESS 64 unordered pieces.

Corynebacterium glutamicum 3-dehydroquinase (aroD) and shikimate Corynebacterium dehydrogenase (aroE) genes, complete cds. glutamnicum Homo sapiens PAC clone DJ10964C1 1 from 7p14-p1 5, complete sequence. Homo sapiens Caenorhabditis elegans clone Y76B12, SEQUENCING IN PROGRESS ~,Caenorhabditls elegans unordered pieces.

C.glutamicum tysl gene for L-lysine permease. Corynebacterium glutamicum 46,667 37,451 37,451 38,627 92,113 93,702 34,221 37,965 37,965 38,796 6-Feb-99 21 -Aug-99 21 -Aug-99 16-OCT- 1999 13-MAR- 1999 13-MAR- 1999 31-Jan-99 03-DEC- 1999 03-DEC- 1999 1 1-Nov-99 22-Nov-98 22-Nov-98 22-:Nov-98 2-Aug-96 22-MAR- 1999 16-OCr- 1999 18-MAY- 1999 18-Apr-98 26-Feb-99 30-Jan-92 00.

54,191 26-MAR- 0 1998 38,227 38,227 38.227 48,826 40,135 39.527 98,237 36,616 37,095 100,000

(D

-4 0 0 0 %0

N

GBHTG1:PFMAL13P GBJ{TG1 :PFMALI 3P GBJN2:EHU89655 GBJIN2:EHU89655 192581 AL049180 192581 AL049180 3219 U89655 3219 U89655 Table 4 (continued) Plasmodium falciparum chromosome 13 strain 3037, SEQUENCING IN PROGRESS in unordered pieces.

Plasmodium falciparum chromosome 13 strain 3037, SEQUENCING IN PROGRESS In unordered pieces.

Entamoeba histolytica unconventional myosin lB mRNA, complete cds.

Entamoeba histolytica unconventional myosin lB mRNA, complete cds.

rxa00262 1197 nca00266 531 rxa00278 1155 rxa00295 1125 rxa00323 1461 rxa00324 3258 rxaOO330 1566 rxa00335 1554 GB-ROAF016190 2939 AF016190 Mus musculus connexin-36 (Cx36) gene, complete ods.

EM-PAT:E09719 3505 E09719 DNA encoding precursor protein of alkaline cellulase.

GB PATE021133 GB-IN1 :CELKO5F6 GBBA1:CG1J43535 GBRO.RNU30789 GB-BA2:CGU31281 GBBA1:BRLBIOBA GB _AT:E03937 GB-BA1 :MTCY427 GB-BA1 :MSGB32CS GBBA1 :MTCV427 GBBA1 :MSGB32CS GB.BA1 :MTCY427 GB OM:BOVE LA GBBAl :CGTHRC GBPAT:J09078 GBBA1 :BLTHRESY

N

GB-BA1 :CGGLNA 3494 36912 2531 3510 1614 1647 1005 38110 36404 38110 36404 38110 3242 3120 3146 1892 3686 E02133 AF040653 U43535 U30789 U31281 D14084 E03937 Z70692 178818 Z70692 L78818 Z70692 J02717 X56037 109078 Z29563 Y1 3221 gDNA encoding alkaline cellulase.

Caenoruabditis elegans cosmid K05F6.

Corynebacterium glutamicum muhtidrug resistance protein (cmr) gene, complete cds.

Rattus norvegicus clone N27 mRNA.

Corynebacteruni glutamicum biotin synthase (bloB) gene, complete cds.

Brevibacterium flavumn gene for biotin synthetase, complete cds.

DNA sequence encoding Brevibacterum flavumn biotin-synthase.

Mycobacterium tuberculosis H37Rv complete genome; segment 99/162.

Mycobacterium leprae cosmid B32 DNA sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 99/162.

Mycobacterium leprae cosmid B32 DNA sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 991162.

Bovine elastin a mRNA, complete cds.

Corynebactenium glutamicum thrC genfe for threonine synthase (EC 4.2.99.2).

Sequence 4 from Patent WO 8809819.

Brevibactenium lactofermentum; ATCC 13869;; DNA (genomic);.

Corynebacterium glutamicum ginA gene.

Plasmodium falciparum Plasmodiumn falciparum Entamoeba histolytica Entamoeba histolytica Mus musculus Bacillus sp.

Bacillus sp.

Caenortiabditis elegans Corynebacterium glutamicum Rattus norvegicus Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Mycobacterium tuberculosis Mycobacterium leprae Mycobacterum tuberculosis Mycobactertum leprae Mycobacterium tuberculosis Bos taurus Corynebacterlum glutamicum Unknown.

Corynebacterium glutamicum Corynebacterlum glutamicum 34,947 11 -Aug-99 34.947 11 -Aug-99 36,496 23-MAY- 1997 37,544 23-MAY- 1997 41.856 9-Feb-99 34,741 08-OCT- 1997 (Rel.

52, Created) 34,741 29-Sep-97 36,943 6-Jan-98 36,658 9-Apr-97 38,190 20-Aug-96 99,111 21-Nov-96 98.489 3-Feb-99 98,207 29-Sep-97 35.615 24-Jun-99 60,917 15-Jun-96 44.606 24-Jun-99 52,516 15-Jun-96 38,079 24-Jun-99 39,351 27-Apr-93 99,808 17-Jun-97 99,617 02-DEC- 1994 99,170 20-Sep-95 100,000 28-Aug-97 GB-BA2:AF005635 1690 Table 4 (continued) AF005635 Corynebactenium glutamnicumn glutamine synthetase (ginA) gene, complete ods.

Corynebacteriu glutamicumn rxa00347 891 GBBAI:MSGB27CS GB-EST27AI45521 7 GB-BA2:SSU30252 38793 624 2891 GB-EST21AA911262 581 rxaOO351 1578 rxa00365 727 rxa00366 480 rxa00367 4653 rxa00371 1917 GB-BA1:MLU1 5187 GB-JN2:AC004373 GB-jN2:AF145653 GB-BA1 :AB024708 GB6BA1 :MTCY1A6 GB-BA1 :SC3A3 G86-A1I AB024708 GB.BA1 :MTCY1A6 GB.,.8A1 :SCA3 GB-BA1 :AB024708 GBBA1 :MTCY1A6 GBBA1 :SC3A3 GB-Vl:SBVORFS GB-EST37:A1967505 36138 72722 3197 8734 37751 15901 8734 37751 15901 8734 37751 15901 7568 380 L78817 Mycobacteriumn leprae cosmid B27 DNA sequence. Mycobacteriumn A1455217 LD21828.3prime LD Drosophila melanogaster embryo p01 2 Drosophila Drosophila mel melanogaster cDNA clone LD21 828 3prime, mRNA sequence.

U30252 Synechococcus PCC7942 nucleoside diphosphate kinase and ORF2 protein Synechococcu genes, complete cds. ORFi protein gene, partial cds, and neutral site I for vector use.

AA911262 oe75a02.sl NCI CGAP-Lu5 Homo sapiens cDNA clone IMAGE:1417418 3' Homo sapiens similar to gb:A1 8757 UROKINASE PLASMINOGEN ACTIVATOR SURFACE RECEPTOR, GPI-ANCHORED (HUMAN);, mRNA sequence.

U1 5187 Mycobacterium leprae cosmid L296. Mycobacterium AC004373 Drosophila melanogaster DNA sequence (P1 DS05273 complete Drosophila mel sequence.

AF145653 Drosophila melanogaster clone GH08860 BcDNA.GH08860 Drosophila mel (BcDNA.GH08860) mRNA, complete cds.

A8024708 Corynebactenium glutamicumn gftB and gltD genes for glutamine 2- Corynebacteriu oxoglutarate aminotransferase large and small subunits, complete cds. glutamicurn Z83864 Mycobacterium tuberculosis H37Rv complete genomne: segment 159/162. Mycobacterium tuberculosis AL109849 Streptomyces coelicolor cosmid 3A3. Streptomyces A3(2) AB024708 Corynebacteriumn glutamicumn gftB and gftD genes for glutamine 2- Corynebacteriu oxoglutarate aminotransferase large and small subunits, complete eds. glutamicum Z83864 Mycobacterium tuberculosis I-37Rv complete genome; segment 159/162. Mycobacteniun tuberculosis AL109849 Streptomyces coelicolor cosmid 3A3. Streptomyces A3(2) AB024708 Corynebacteriumn glutamicumn gltB and gltD genes for glutamine 2- Corynebacteriu oxoglutarate aminotransferase large and small subunits, complete cds. glutamnicumn Z83864 Mycobacterium tuberculosis H37Rv complete genome; segment 159/162. Mycobacteriumr tuberculosis AL1 09849 Streptomyces coelicolor cosmid 3A3. Streptomyces A3(2) M89923 Sugarcane bacilliform virus ORF 1 ,2,and 3 DNA, complete cds. Sugarcane bac A1967505 Ljimpest03-215-clO Ljirnp Lambda HybriZap two-hybrid library Lotus Lotus ]aponicu japonicus cDNA clone LP21 5-b3-0lO 5'similar to 60S ribosomal protein L39, mRNA sequence.

AF043700 Caenortiabditis elegans cosmid K09H9. Caenorhabditis m leprae anogaster sPCC7942 98,906 1-4-Jun-99 0 66,345 15-Jun-96 S 00 34,510 09-MAR- 1999 37,084 29-OCT- 1999 37,500 21-Apr-98 leprae lanogaster anogaster m coelicolor coelicolor im coelicolor 52,972 46,341 49,471 96,556 39.496 37.946 99,374 41.333 37.554 99,3 12 36.971 37,905 09-MAR- 1995 1 7-Jul-98 14-Jun-99 13-MAR- 1999 17-Jun-98 16-Aug-99 13-MAR- 1999 1 7-Jun-98 16-Aug-99 13-MAR- 1999 17-Jun-98 16-Aug-99 12-Jun-93 24-Aug-99 (0 (0 0 0 0 \0 illiform virus 35,843 s 42,593 GB-IN1:CELK09H9 37881 elegans 34,295 22-Jan-98 rxa00377 1245 GBBAI:CCU13664 GB_L1 ANSDGENE GB_GSS4AQ730303 rxa00382 1425 GB_BAl :PAHEML GBBA1 :MTY25D10 OB-BAI :MSGY224 rxa00383 1467 GB-BA1 :MLC81222 GBJHTG2AC006269 GB-HTG2AC007638 rxaOO391 843 GBEST38AW0117OS 3 GBPAT:AR065852 GB _V1AF148805 rxa00393 1017 GB-BA1:MTY25D1O GB-BA1 :MSGV224 GB BA1 :LB1 306 rxa00402 623 GB-BA2:AF052652 GBBAIF109162 GB-BA2:AF09291 8 rxaOO403 1254 GB-BA2AFOS2652 GBBAIW:TVO1 6 1678 1299 483 4444 40838 40051 34714 167171 178053 613 32207 28559 40838 40051 7762 2096 4514 20758 2096 53662 Table 4 (continued) U13664 Caulobacter crescentus uroporphyrinogen decarboxylase homolog (hemE) gene, partial cds.

Y08866 A.nidulans sD gene.

AQ730303 HS55505_B1_C04.37A RPCI-1 1 Human Male SAC Library Homo sapiens genomic clone Plate=1 081 Col=7 Row=F, genomic survey sequence.

X82072 P.aeruginosa hemL gene.

Z95558 Mycobacterium tuberculosis H37Rv complete genome; segment 28/162.

AD000004 Mycobacteriumn tuberculosis sequence from clone y 224 AL049491 Mycobacteriumn leprae cosmid B1222.

AC006269 Homo sapiens chromosome 17 clone hRPK.515-E_23 map 17, SEQUENCING IN PROGRESS 2 ordered pieces.

AC007638 Homo sapiens chromosome 17 clone hRPK.5150)7 map 17, SEQUENCING IN PROGRESS 8 unordered pieces.

AW017053 EST272398 Schistosoma mansoni male, Phil LoVerde/Joe Merrick Schistosomna mansoni cDNA clone SMMAS14 5Vend, mRNA sequence.

AR065852 Sequence 20 from patent US 5849564.

AF148805 Kaposi's sarcoma-associated herpesvirus ORF 68 gene, partial cds; and ORF 69, kaposin, v-FLIP. v-cyciin, latent nuclear antigen, ORF K14, v-GPCR, putative phosphoribosyformylglycinamidine synthase. and LAMP (LAMP) genes, complete cds.

Z95558 Mycobactenium tuberculosis H37Rv complete genome; segment 28/162.

AD000004 Mycobacterium tuberculosis sequence from clone y224.

Y1 3803 Mycobactenumr leprae cosmid B1306 DNA.

AF052652 Corynebacterium glutamnicumn homoserine 0-acetyltransferase (metA) gene, complete ods.

AF109162 Corynebactenumr diphtheriae heme uptake locus, complete sequence.

AF092918 Pseudomonas alcaligenes outer membrane Xcp-secretion system gene cluster.

AF052652 Corynebacterium glutamicum homoserine 0-acetyltransferase (metA) gene, complete cds.

AL021841 Mycobacterium tuberculosis H37Rv complete genome; segment 1431162.

Caulobacter crescentus Emericella nidulans Homo sapiens Pseudomonas aeruginosa Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacteriumn leprae Homo sapiens Homo sapiens Schistosoma mansoni Unknown.

Kaposi's sarcomaassociated herpesvirus Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacterium leprae Corynebacterium glutamicum Corynebacteriumn diphtheriae Pseudomonas alcaligenes Corynebacterium glutamicum Mycobacterium tuberculosis 36,832 39,603 36,728 54.175 61,143 61,143 43,981 35,444 34,821 40.472 38.586 38.509 36.308 39,282 39,228 99,672 40,830 50.161 99,920 52,898 37.565 24-MAR- 1995 1 7-OCT- 1996 15-Jul-99 I18-DEC- 1995 17-Jun-98 03-DEC- 1996 27-Aug-99 10-Jun-99 22-MAY- 1999 10-Sep-99 29-Sep-99 2-Aug-99 1 7-Jun-98 03-DEC- 1996 24-Jun-97 19-MAR- 1998 8-Jun-99 06-DEC- 1998 19-MAR- 1998 23-Jun-99 31-Aug-98 t4 GB-EST23:AI 111288 750 All111288 SWOvAMCAQO2AO5SK Onchocerca volvulus adult male cDNA (SAW98MLW-Onchocerca volvulus OvAtv) Onchocerca volvulus cONA clone SWOvAMCAQO2AO5 5' mRNA sequence.

rxa00405 613 rxaOO42O 1557 rxa00435 1296 rxa00437 579 rxa00439 591 rxaOO4.40 582 rxa00441 1287 rxa00446 987 GB-BA1 :MTV016.

GBR4AC0O5145 GBBA1:MTV016 GB BAt :MTY13DI2 GB-BA1 :MSGV126 GB,.BA1:MSGB971 C

S

GBBAI :AFACBBTZ GB-HTG4:AC009541 GBHTG4:AC009541 GB-PR4AC005951 GBBA1:SC2A1 1 GBR4:AC005951 GBBA1:MTVOI6 GSBPL2:AF167358 GBJ-TG3:AC0091 20 GB..BA2:SKZ861 11 GBBA1 :SC2E1 GB BA1 :SC2E1 GByPR2:HS173D1 GB-HTG2:HSDJ71 9K 3 GB-HTG2:HSDJ7I 9l 3 GB BA1:SCD78 GB..HTG4:AC009367 GBHTG4:AC009367 53662 AL021841 143678 AC005145 53662 AL021 841 37085 Z80343 37164 AD000012 37566 178821 2760 M68904 169583 AC009541 169583 AC009541 155450 AC005951 22789 AL031184 155450 AC005951 53662 AL021 841 1022 AF167358 269445 AC009120 7860 Z861 11 38962 AL023797 38962 AL023797 117338 AL031984 267114 AL109931 267114 AL109931 36224 AL034355 226055 AC009367 226055 AC009367 Table 4 (continued) Mycobacterium tuberculosis H37Rv complete genome; segment 143/1 62.

Homo sapiens Xp22.166-169 GSHB-523A23 (Genome Systems Human BAC library) complete sequence.

Mycobacterium tuberculosis H37Rv complete genome: segment 143/162.

Mycobacterium tuberculosis H37Rv complete genome; segment 156/162.

Mycobacterium tuberculosis sequence from clone y126.

Mycobacterium leprae cosmid 6971 DNA sequence.

Alcaligenes eutrophus chromsomal transketolase (cbbTc) and phosphoglycolate phosphatase (cbbZc) genes, complete cds.

Homo sapiens chromosome 7, -SEQUENCING IN PROGRESS ~,25 unordered pieces.

Homo sapiens chromosome 7, -SEQUENCING IN PROGRESS ,25 unordered pieces.

Homo sapiens chromosome 17, rlon~e hRPK.372j(320, complete sequence.

Streptomyces coelicolor cosmid 2A1 1.

Homo sapiens chromosome 17, clone hRPK.372j(..20, complete sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 143/162.

Rumex acetosa expansin (EXP3) gene, partial cds.

Homo sapiens chromosome 16 clone RPCI-1 1484E3, -SEQUENCING IN PROGRESS 34 unordered pieces.

Streptomyces lividans rpsP, trmD, rpIS, sipW, sipX, sipY. sipZ, mutT genes and 4 open reading frames.

Streptomyces coelicolor cosmid 2E1.

Streptomyces coelicolor cosmid 2E1.

Human DNA sequence from clone 173DI on chromosome Ilp 3 .6.

2 1l- 36.33.Contains ESTs, STSs and GSSs, complete sequence.

Homo sapiens chromosome Xcone RP4-719K3 pq21.1- 2 .31, SEQUENCING IN PROGRESS in unordered pieces.

Homo sapiens chromosome X clone RP4-719K3 map q21.1-21.31, SEQUENCING IN PROGRESS in unordered pieces.

Streptomyces coelicolor cosmid D78.

Drosophila melanogaster chromosome 3117A2 clone RPCI98-48B15, SEQUENCING IN PROGRESS 44 unordered pieces.

Drosophila melanogaster chromosome 3L/76A2 clone RPC198-48BI5.

SEQUENCING IN PROGRESS 44 unordered pieces.

Mycobactenumr tuberculosis Homo sapiens Mycobactenumn tuberculosis Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacterium leprae Raistonia eutropha Homo sapiens Homo sapiens Homo sapiens Streptomyces coelicolor Homo sapiens Mycobacterium tuberculosis Rumex acetosa Homo sapiens Streptomyces; lividans Streptomyces coelicolor Streptomyces coelicolor Homo sapiens Homo sapiens Homo sapiens Streptomyces coelicolor Drosophila melanogaster Drosophila melanogaster 57,259 34,179 40,169 62,031 61,902 39,651 38.677 36,335 36.335 31,738 43,262 37,647 37,088 4.6.538 43,276 43,080 42,931 36,702 38,027 34,521 34,521 56,410 34.959 34,959 23-Jun-99 08-DEC- 1998 23-Jun-99 17-Jun-98 IlO-DEC- 1996 15-Jun-96 27-Jul-94 12-OCT.

1999 12-OCT.

1999 1 8-Nov-98 5-Aug-98 18-Nov-98 23-Jun-99 17-Aug-99 3-Aug-99 27-OCT- 1999 4-Jun-98 4-Jun-98 23-Nov-99 03-DEC- 1999 03-DEC- 1999 26-Nov-98 16-OCT- 1999 16-OCT- 1999 rxa00448 1143 rxa00450 424 GB-PR3:AC003670 GB-HTG2:AF029367 GB-HTG2:AF029367 GB-HTG2:AC007824 Table 4 (continued) 88945 AC003670 Homo sapiens 12q13.1 PAC RPCI1-130F5 (Roswell Park Cancer Institute Human PAC library) complete sequence.

Homo sapiens 35.682 9-Jun-98 148676 148676 133361 AF029367 AF029367 AC007824 GB-HTG2:AC007824 133361 AC007824 GB-EST35AIS18057 412 A1818057 Homo sapiens chromosome 12 clone RPCI-1 130F5 map 12q13.1, Homo sapiens SEQUENCING IN PROGRESS 156 unordered pieces.

Homo sapiens chromosome 12 clone RPCI-1 130F5 map 12q1 3. 1, Homo sapiens SEQUENCING IN PROGRESS 156 unordered pieces.

Drosophila melanogaster chromosome 3 clone BACRO2L_16 (D715) RPCI-98 Drosophila mel 021L.16 map 89E-90A strain y; cn bw sp, SEQUENCING IN PROGRESS 91 unordered pieces.

Drosophila melanogaster chromosome 3 clone BACRO2LI6 (0715) RPCI-98 Drosophila mel 021L.16 map 89E-90A strain y; cn bw sp, -SEQUENCING IN PROGRESS 91 unordered pieces.

wkl4aO8.xl NCI_CGAPLym12 Homo sapiens cDNA clone IMAGE:2412278 Homo sapiens 3' similar to gb:V00764 UBIQUINOL-CYTOCHROME C REDUCTASE 11 KD PROTEIN (HUMAN);, mRNA sequence.

Mycobacterium leprae cosmid B1779. Mycobacterium Drosophila melanogaster cosmid clone 86E4. Drosophila meL 927P1 -2H3.TP 927PI Trypanosoma brucei genomic clone 927P1 -21-3. Trypanosoma b genomic survey sequence.

anogaster anogaster 31,373 31,373 40,000 40,000 18-OCT.

1997 18-OCT- 1997 2-Aug-99 2-Aug-99 35,714 24-Aug-99 rxaOO461 975 GBBA1 :MLCB1779 GBjN1 :DMC86E4 GBGSS1 5:AQ64032 43254 29352 467 Z98271 AL021 086 AQ640325 Ieprae anogaster rucei 39,308 37,487 38.116 8-Aug-97 27-Apr-99 8-Jul-99 rxa0045 rxa00487 1692 GBBA1:BAGUAA GB6BA2:U0001 5 GBBA11:MVTCY78 rxa00488 1641 GB-BA1:MTCY78 GBBA2:U00015 GB8BA1 :SCAJ 1106011 rxaD0489 1245 GB.BA2:U00015 GB-HTG2:HS225E12 GB-HTG2:HS225E1 2 rxa00533 1155 GB8BA1:CGLYS 3866 42325 33818 33818 42325 4692 42325 126464 126464 2803 Y10499 UOO015 Z77165 Z77165 U00015 AJO10601 UOOO1 5 AL031772 AL031772 X57226 B.ammoniagenes guaA gene.

Mycobacterium Ieprae cosmid B1620.

Mycobacterium tubercujlosis H37Rv complete genome; segment 145/162.

Mycobacterium tuberculosis H37Rv complete genome: segment 1451162.

Mycobacterium leprae cosmid 81620.

Streptomyces coelicolor A3(2) DNA for whiD and whiK loci.

Mycobactenumr Ieprae cosmid 81620.

Homo sapiens chromosome 6 clone RP1-225E12 map q24, SEQUENCING IN PROGRESS in unordered pieces.

Homo sapiens chromosome 6 clone RP1-225E12 map q24, SEQUENCING IN PROGRESS in unordered pieces.

C. glutamicum lysC-alpha, lysC-beta and asd genes for aspartokinase-alpha and -beta subunits, and aspartate beta semnialdehyde dehydrogenase, respectively (EC 2.7.2.4; EC 1.2.1.11).

Corynebacterium ammoniagenes Mycobacterium leprae Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacterium leprae Streptomyces coelicolor Mycobacterium leprae Homo sapiens Homo sapiens Corynebacterium glutamicum 74,259 37,248 39,725 39,451 39,178 60.835 38,041 36,756 36,756 99,913 8-Jan-98 01-MAR- 1994 17-Jun-98 17-Jun-98 01-MAR- 1994 17-Sep-98 0 1 -MAR- 1994 03-DEC- 1999 03-DEC- 1999 17-Feb-97 Table 4 (continued) X82928 C.glutamicum aspartate-semialdehyde dehydrogenase gene.

GBBA1 :CGCVSCAS 1591 0 GB PAT:A07546 GB-BA1:CGLVS rxa00534 1386 GBBA1:CORASKD GB-PAT:E 14514 rxa00536 1494 GB-BAI:CGLEUA GB-BA1:MTVO25 GBBA1 :MTU88526 rxa00537 2409 GBBA2:SCD25 GB-B6A1:MTCV7H7A GB-BAI :MTh 34956 rxaOO54l 792 GBPAT:192052 GB-BA1 :MTCY369 nca00558 1470 GBA1:BAPURF GB-BAI :MLU1 5182 GB-BAI :MTCY7H7A rxa00579 1983 GB-PAT:AR016483 EM.PATE1 1273 2112 A07546 Recombinant DNA fragment (Pstl-Xhol).

2803 X57226 C. glutamicumn lysO-alpha, IysC-beta and asd genes for aspartokinase-aipha and -beta subunits, and aspartate beta semnialdehyde dehydrogenase, respectively (EC EC 1.2.1.11).

2957 116848 Corynebacterium flavumn aspartokinase (ask), and aspartate-semialdehyde dehydrogenase (asd) genes, complete cds.

1643 E14514 DNA encoding Brevibactedrlu aspartokinase.

3492 X70959 C.glutamicum gene leuA for isopropylmalate synthase.

121125 AL022121 Mycobacterium tuberculosis H37Rv complete genome; segment 155/162.

2412 U88526 Mycobactenium tuberculosis putative alpha-isopropyll malate synthase (leuA) gene, complete cds.

41622 ALI1I8514 Streptomyces coelicolor cosmid 025.

10451 Z95618 Mycobacteriumn tuberculosis H37Rv complete genome; segment 39/162.

2462 U34956 Mycobacterium tuberculosis phosphoribosylformylglycinamidinie synthase (purL) gene, complete ods.

2115 192052 Sequence 19 from patent US 5726299.

38109 Z95151 Mycobacterlum leprae cosmid B5.

36850 Z80226 Mycobactenium tuberculosis H37Rv complete genome; segment 36/162.

1885 X91252 Bammoniagenes purF gene.

40123 U15182 Mycobacterium leprae cosmid B2266.

10451 Z95618 Mycobacterium tuberculosis H137Rv complete genome; segment 391162.

2104 AR016483 Sequence 1 from patent US 5776740.

2104 E 1273 DNA encoding senine hydroxymethyl transferase.

Corynebacterium glutamicum synthetic construct Corynebacterium glutamicumn Corynebacterium flavescens Corynebacterium glutamicum Corynebacterium glutamicum Mycobacterium tuberculosis Mycobacterium tuberculosis Streptomyces coelicolor A3(2) Mycobactenumr tuberculosis Mycobacterium tuberculosis Unknown.

Mycobacterium leprae Mycobacterium tuberculosis Corynebactenium ammoniagenes Mycobacterium leprae.

Mycobacterium tuberculosis Unknown.

Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum 99,221 17-Feb-97 99.391 99,856 98.701 98,773 100,000 68,003 68,185 63,187 62,401 62,205 98,359 62,468 60.814 66,095 64,315 64,863 98,810 98,810 98,810 99.368 30-Jul-93 17-Feb-97 11 -Jun-93 28-Jul-99 10-Feb-99 24-Jun-99 26-Feb-97 21 -Sep-99 17-Jun-98 28-Jan-97 Ol-DEC- 1998 24-Jun-97 17-Jun-98 5-Jun-97 09-MAR- 1995 17-Jun-98 1998

OB-OCT-

1997 (Rel.

52, Created) 24-Jun-98 24-Jun-98 GB-PAT:E1 2594 GB-PAT:E12594 2104 E12594 2104 E12594 DNA encoding serine hydroxymethyltransferase from Brevibacterium flavumn.

DNA encoding serine hydroxymethyltransferase from Brevibactenium flaum.

rxa0O580 1425 Table 4 (continued) AR016483 Sequence 1 from patent US 5776740. GB-PATARO1 6483 2104 EM-PAT:E1 1273 2104 Unknown.

El 1273 rxa00581 1092 rxa00584 1248 rxaOO6lB 1230 GB _PATE12594 EM!PAT:E1 1273 2104 E12594 2104 El 1273 GBATAR016483 2104 GB-BAl:CORAHPS 2570 GB-BA1AOPCZA361 37941 GB-.BA1:D90714 14358 GB-EST19:AA802737 280 GB._ESTM8A15343811 581 GBIN1:DMANILLIN rxaOO6l9 1551 GB-BA1:MTCY369 GB BA1:MLCB5 GBPAT:A60305 rxa00620 1014 GB-PL2:AF063247 GB BA1 :STMAPP GBHTG3:AC008763 rxa00624 810 GB.,jNI:CEY41E3 GBEST13:AA362167 GBIN1 :CEY41 E3 rxa00626 1386 GB-BA1 :MTCY369 GB,_BAI:MLU 15187 4029 36850 38109 1845 1450 2069 214575 150641 372 150641 36850 38109 36138 AR01 6483 L07603 AJ223998 D90714 AA802737 A1534381 X89858 Z80226 Z95 151 A60305 AF063247 M91546 AC008763 Z95559 AA3621 67 Z95559 Z80226 Z95151 U15187 DNA encoding serine hydroxymethyl transferase. Corynebacterium glutamicum DNA encoding serine hydroxymethyltransferase from Brevibacterium flavumn. Corynebacterium glutamicum DNA encoding serine hydroxymethyl transferase. Corynebactenium glutamicum Sequence 1 from patent US 5776740. Unknown.

Corynebactenium glutamicum 3-deoxy-D-arabinoheptulosonate-7-phosphate Corynebactenium synthase gene, complete cds. glutamicum Amycolatopsis orientalis cosmid PCZA36I. Amycolatopsis orientalis Eschenichia coli genomic DNA. 17.1 min). Escherichia coli GM06236.Sprime GM Drosophila melanogaster ovary BlueScript Drosophila Drosophila melanogaster melanogaster cDNA clone GM06236 5prime, mRNA sequence.

SD07186.5prime SD Drosophila melanogaster Schneider L2 cell culture pOT2 Drosophila melanogaster Drosophila melanogaster cDNA clone SD071 86 5prime similar to X89858: AN FBgnO0l 1558 PID:g927407 SPTREMBL:024240, mRNA sequence.

D.melanogaster mRNA for anillin protein. Drosophila melanogaster Mycobacterium tuberculosis H37Rv complete genome; segment 36/162. Mycobacterium tuberculosis Mycobacterium leprae cosmid B5. Mycobacterium Ieprae Sequence 5 from Patent W09708323. unidentified Pneumocystis carinii f. sp. ratti enolase mRNA, complete cds. Pneumnocystis carinii f. sp.

ratti Streptomyces lividans aminopeptidase P (PepP) gene, complete cds. Streptomyces livid ans Homo sapiens chromosome 19 clone CITB-E1 3214H19, -SEQUENCING Homo sapiens IN PROGRESS 21 unordered pieces.

Caenorhabditis elegans cosmid Y41 E3. complete sequence. Caenortiabditis elegans EST71561 Macrophage I Homo sapiens cDNA 5Vend, mRNA sequence. Homo sapiens Caenorhabditis elegans cosmid Y41 E3, complete sequence. Caenorhabditis elegans Mycobacterium tuberculosis H37Rv complete genome; segment 36/162. Mycobacterium tuberculosis Mycobacterium leprae cosmid B5. Mycobacteriumn Ieprae Mycobacterium Ieprae cosmid L296. Mycobacteriumn leprae 34,398 62,776 61.831 61,785 41,060 37.126 40.020 36,986 38,378 37,694 57,971 58,806 38,007 8-Nov-95 17-Jun-98 24-Jun-97 06-MAR- 1998 5-Jan-99 12-Jun-93 3-Aug-99 2-Sep-99 21-Apr-97 2-Sep-99 17-Jun-98 24-Jun-97 09-MAR- 1995 99.368 1998 99,368 08-OCT- 1997 (Rel.

52, Created) 37.071 24-Jun-98 37.071 08-OCT- 1997 (Rel.

52, Created) 37.071 1998 98,236 26-Apr-93 54.553 29-MAR- 1999 53.312 7-Feb-99 39,928 25-Nov-98 41,136 18-MAR- 1999 0~ 0 0 0 ce rxa00632 795 rxa00633 1392 rxa00688 666 rxa00708 930 rxaOO717 1083 rxa0071 8 831 rxa00727 1035 GBBA1:BRLBIOAD 2272 GB _AT:E04041 GBPAT:IE04040 GB-BAI :BRLB lOAD GBYPAT:E04040 GB8BA2:EHU38519 GB-BA1:MTV041 GB..BA1:BRLSECY GBBA2:MBU77912 GB,_BA2:AF1 57493 GB-PAT:I00836 GB PAT:E0031 1 GB PAT:17 8753 GByPAT:192042 GB-BA1:MTCI125 GB..BA1 :MTC 1125 GB..BAI1MTC 1125 GB-GSS1 2AQ42075 GB-ITG3:AC008332 675 1272 2272 1272 1290 28826 1516 7163 25454 1853 1853 1187 1187 37432 37432 37432 671 118545 Table 4 (continued) D14083 Brevibacterium flavumngenes for 7,8-diaminopelargonic acid aminotransferase Corynebacterium and dethiobiotin synthetase, complete cds. glutamicum E04041 DNA sequence coding for desthiobiotinsynthetase. Corynebacterium glutamicum E04040 DNA sequence coding for diamino pelargonic acid aminotransferase. Corynebacterium glutamicum D14083 Brevibacterium flavumn genes for 7,8-iaminopelargonic acid aminotransferase Corynebacterium and dethiobiotin synthetase, complete cds. glutamicum E04040 DNA sequence coding for diamino pelargonic acid aminotransferase. Corynebacterium glutamicum, U3851 9 Erwinia herbicola adenosylmethionine-8-mino-7-oxononanoate transaminase Erwinia lherbicola (bioA) gene, complete cds.

AL021958 Mycobacterium tuberculosis H37Rv complete genome; segment 35/162. Mycobacterium tuberculosis D14162 Brevibactenium flavumn gene for SecY protein (complete cds) and gene or Corynebacterium adenylate kinase (partial cds). glutamicum U77912 Mycobacterium bovis MBE50a gene, partial cds; and MBE5Ob. MBE50c, Mycobacterium bovis preprotein translocase SecY subunit (secY), adenylate kinase (adk), methionine aminopeptidase (map), RNA polymerase ECF sigma factor MBE50d, and MBE50e genes, complete cds.

AF157493 Zymomonas mobilis ZM4 fosmid clone 42D7, complete sequence. Zymomonas mobilis 100836 Sequence 1 from Patent US 4758514. Unknown.

E0031 1 DNA coding of 2,5-diketogluconic acid reductase. unidentified 178753 Sequence 9 from patent US 5693781. Unknown.

192042 Sequence 9 from patent US 5726299. Unknown.

Z98268 Mycobacterium tuberculosis H37Rv complete genomne; segment 76/162. Mycobacterium tuberculosis Z98268 Mycobacterium tuberculosis H37Rv complete genome; segment 76/162. Mycobacterium tuberculosis Z98268 Mycobacterium tuberculosis H37Rv complete genome: segment 76/162. Mycobacterium tuberculosis AQ420755 RPCI-1 1-1 68G18.TJ RPCI-11I Homo sapiens genomic clone RPCI-1 1- Homo sapiens 1681318, genomic survey sequence.

AC008332 Drosophila melanogaster chromosome 2 clone BACR48D1O (D867) RPCI-98 Drosophila melanogas 48.D.1 map 34A-34A strain y: cn bw sp, -SEQUENCING IN PROGRESS '78 unordered pieces.

AC008332 Drosophila melanogaster chromosome 2 clone BACR48DI10 (D867) RPCI-98 Drosophila melanogas 48.D.10 map 34A-34A strain y; cn bw sp, -SEQUENCING IN PROGRESS-, 78 unordered pieces.

98,074 93.814 95,690 95,755 55,564 60,030 99,563 60,030 39,116 47,419 47,419 37,814 37,814 50,647 55,228 40,300 35,750 40.634 97,358 3-Feb-99 ter 29-Sep-97 29-Sep-97 3-Feb-99 29-Sep-97 4-Nov-96 17-Jun-98 3-Feb-99 27-Jan-99 5-Jul-99 21-MAY- 1993 29-Sep-97 3-Apr-98 Ol-DEC- 1998 17-Jun-98 17-Jun-98 17-Jun-98 23-MAR- 1999 6-Aug-99 6-Aug-99 GBJ-1TG3AC008332 118545 ter 40,634 GB-HTG3:AC0O8332 118545 AC008332 rxa00766 966 rxa00770 1293 rxa00779 1056 rxaOO780 669 rxa00838 1023 rxa00863 867 rxa00864 873 GB-HTG2:AC006789 GBTG2AC006789 GBBA1 :090810 GB-BA1 :MV043 GB-BAI:MLU 15182 GB-BA2:SCD25 GBTGI :CERO8A5 GBHTG1:CER08A5 GBPL2:AF078693 GBBA1:MTCY98 GB-BA1:AVINIFREG GBBA2:AF0O11780 GB-EST1 :Z30506 GB-PL2:AC006258 GBESTV7A1998439 GB-BA1:BLDAPAB GByAT:E16749 GB-PAT:E14520 GB-BAI :BLDAPAB GB-BA1 :CGDAPB 83823 83823 20476 68848 40123 41622 51920 51920 1492 31225 7099 6701 329 110469 455 3572 2001 2001 3572 1902 AC006789 AC006789 D9081 0 AL022004 U 15182 AL1118514 Z82281 Z82281 AF078693 Z83860 M60090 AF001 780 Z30506 AC006258 A1998439 Z21502 El16749 E14520 Z21 502 X67737 Table 4 (continued) Drosophila melanogaster chromosome 2 clone BACR48DIO (D867) RPCI-98 Drosophila melanogaster 48.03.10 map 34A-34A strain y; cn bw sp, -SEQUENCING IN PROGRESS-, 78 unordered pieces.

Caenorhabditls elegans clone Y49IF6, -SEQUENCING IN PROGRESS ~,Caenorhabditis elegans 2 unordered pieces.

Caenorhabditis elegans clone Y49F6, SEQUENCING IN PROGRESS ~,Caenortiabditis elegans 2 unordered pieces.

Ecoli genomic DNA, Kohara clone #319(37.4-37.8 min.). Escherichia coli Mycobacterium tuberculosis H37Rv complete genome; segment 40/1 62. Mycobacterium tuberculosis Mycobacteriumn leprae cosmid B2266. Mycobacterium leprae Streptomyces coelicolor cosmid D25. Streptomyces coelicolor A3(2) Caenorhabdilis elegans chromosome V clone ROWA, -SEQUENCING IN Caenorhabditis elegans PROGRESS in unordered pieces.

Caenorhabditis elegans chromosome V clone ROWA, -SEQUENCING IN Caenortiabditis elegans PROGRESS in unordered pieces.

Chlamydomonas reinhardtii putative O-acetylserine(thiol~lyase precursor Chlamydomonas relnhardti (Crcys-1A) mRNA. nuclear gene encoding organellar protein, complete cds.

Mycobactenumr tuberculosis H37Rv complete genome; segment 103/162. Mycobacterium tuberculosis Azotobacter chroococcum nifU, nifS, nifV, nifP, nifW, nifZ and nitM genes, Azotobacter cliroococcum complete cds.

Cyanothece PCC 8801 NifP (nifP), nitrogenase (nifBl). FdxN (fdxN). NifS (nifS) Cyanothece PCC8801 and NifU (nifU) genes, complete cds, and NifH- (nifH) gene, partial cds.

ATS2430 AC16H Arabidopsis thaliana cDNA clone TA1306 3, mRNA Arabidopsis thaliana sequence.

Arabidopsis thaliana BAC Fl 8G1 8 from chromosome V near 60.5 cM, Arabidopsis thaliana complete sequence.

701545695 A. thaliana, Columbia Col-0, rosette-2 Arabidopsis thaliana cONA Arabidopsis thaflana clone 701545695, mRNA sequence.

BlIactofermentum dapA and dapB genes for dihydrodipicolinate synthase and Corynebacterium dihydrodipicolinate reductase. glutamicum gDNA encoding dihydrodipicolinate synthase (DDPS). Corynebacterium glutamicum DNA encoding Brevibacterium dihydrodipicolinic acid synthase. Corynebacterium 33.888 36,737 36,737 36.526 66,193 61,443 59,938 64.896 64.896 57,970 54,410 51,729 36,309 44.308 35,571 36,044 99,539 99.539 99.539 99,885 100,000 6-Aug-99 25-Feb-99 25-Feb-99 29-MAY- 1997 24-Jun-99 09-MAR- 1995 21 -Sep-99 14-OCT- 1998 14-OCT- 1998 3-Nov-99 17-Jun-98 26-Apr-93 08-MAR- 1999 11 -MAR- 1994 28-DEC- 1998 8-Sep-99 1 6-Aug-93 28-Jul-99 28-Jul-99 16-Aug-93 1 -Apr-93 glutamicum BlIactofermentum dapA and dapB genes for dihydrodipicolinale synthase and Corynebactenium dihydrodipicolinate reductase. ,glutamicum C.glutamicum dapB gene for dihydrodipiolinate reductase. Corynebacterium glutamicum rxa00865 1026 rxa00867 650 rxa00873 779 rxa00884 1263 rxa00891 1102 rxa00952 963 rxaOO954 644 rxa00955 1545 GByAT:E14520 2001 GBBAl :BLDAPAB 3572 GBPAT:E16752 1411 GB PAT:AR0381 13 1411 GB,,BA1:MTVOO2 56414 GBBAMLCB22 40281 GB-BA1:SAU19858 2838 GB-BA1:SC0001206 9184 GB-BA1 :SCOOOI 205 9589 GB.,BA1:D78198 2304 GB-BA1 :MTCY253 41230 GB...BA1MSGY222 41156 GB-,GSS15AQ65460 468 0 GB-BA1:MTCI418B 11700 GB-BA1:SC0001206 9184 GB-BA1:SCO0O12OS 9589 EM.PAT:E10963 3118 GB,_BA11:13LTRIP 7725 GB...PAT:EO11688 7725 GBPAT:EO1 375 7726 GB PAT:E01 688 7725 GB-BA1:BLTRP 7725 GB-PAT:EO1 375 7726 E 14520 Z21 502 E 16752 AR0381 13 AL008967 Z98741 U19858 AJ001206 AJO01 205 D78198 Z81 368 ADOOOO1 0 A0654600 Z96071 AJO01 206 AJ001205 E10963 X04960 E01688 E01375 E01688 X04960 E01375 Table 4 (continued) DNA encoding Brevibacterium dihydrodipicolinic acid synthase. Corynebactenium glutamicum Blactofermentum dapA and dapB genes for dihydrodipicolinate synthase and Corynebacterium dihydrodipicolinate reductase. glutamicum gDNA encoding dihydrodipicolinate reductase (DDPR). Corynebacterium glutamicum Sequence 18 from patent US 5804414. Unknown.

Mycobacterium tuberculosis H37Rv complete genome; segment 122/162. Mycobacterium tuberculosis Mycobacterium leprae cosmid B22. Mycobacterium Ieprae Streptomyces antibioticus guanosine pentaphosphate synthetase (gpsl) gene, Streptomyces; antibioticus complete cds.

Streptornyces coelicolor A3(2), glycogen metabolism cluster 11. Streptornyces coelicolor Streptomyces coelicolor A3(2) glycogen metabolism clusteri. Streptomyces coelicolor Pimelobacter sp. DNA for tre ha lose synthase. complete cds. Pimelobacter sp.

Mycobacterium tuberculosis H37Rv complete genome; segment 106/1 62. Mycobacterium tuberculosis Mycobacterlum tuberculosis sequence from clone y 222 Mycobacterium tuberculosis Sheared DNA-1014.TF Sheared DNA Trypanosoma brucel genomic clone Trypanosoma brucei Sheared DNA-1014, genomic survey sequence.

Mycobacterium tuberculosis H-37Rv complete genome; segment 7/162. Mycobacterium tuberculosis Streptomyces coelicolor A3(2), glycogen metabolism cluster 11. Streptomyces coelicolor Streptomyces coelicolor A3(2) glycogen metabolism clusteri. Streptomyces coelicolor gONA encoding tryptophan synthase. Corynebacterlum glutamicum Brevibactenum lactofermentumn tryptophan operon. Corynebacterium glutamicum Genomic DNA of trp operon of prepibacterium latophelmentamn. unidentified DNA sequence of tryptophan operon. Corynebacterium glutamicum Genomic DNA of trp operon of prepibactenium latophelmentamn. unidentified Brevibactenum lactofermentumn tryptophan operon. Corynebacterium glutamicum DNA sequence of tryptophan operon. Corynebacterium glutamicum 100,000 100.000 99,805 99,805 39,179 39,482 69.706 63,415 61,617 60,594 37,785 38,006 33,974 63.297 61,965 61,727 99,688 98,847 98,428 98,758 98,758 98,758 98.372 28-Jul-99 1 6-Aug-93 28-Jul-9 29-Sep-99 17-Jun-98 22-Aug-97 1996 29-MAR- 1999 29-MAR- 1999 5-Feb-99 17-Jun-98 03-DEC- 22-Jun-99 18-Jun-98 29-MAR- 1999 29-MAR- 1999 08-OCT- 1997 (Rel.

52. Created) 10-Feb-99 29-Sep-97 29-Sep-97 29-Sep-97 10O-Feb-99 29-Sep-97 00

W

GB-BA1 :BLTRP GB PAT:E01688 ncaOO956 1237 EM-PAT:E10963

GBBAI:BL-TRP

GByAT:EO11375 rxa00957 1677 GBBAI:BLTRP GB-PAT.E01 375 GB PAT:E01 688 rxa00958 747 GB-BA1 :BLTRP GB-PAT:EO1 375 GB.YAT:E01688 rxa00970 1050 GBBAI:CGHOMTHR GB-PAT:109077 GB PAT: EO1 358 rxa00972 1458 GB-PAT:E16755 GkPATAR0381 10 GB-PAT: E 14508 rxaOO98l 753 GB OV:GGA245664 GBPL2:AC007887 GB-GSS1 ;CNS0ORN

W

7725 7725 3118 7725 7726 7725 7726 7725 7725 7726 7725 3685 3685 2615 3579 3579 3579 512 159434 542 63033 3619 63033 X04960 E01 688 E10963 X04960 EO1 375 X04960 E01 375 E01 688 X04960 E01 375 E01688 Y00546 1097 E01 358 E 16755 AR0381 10 E14508 AJ245664 AC007887 AL087338 AL021 246 Y13070 AI-021246 Table 4 (continued) Brevibacterium lactofermentum tryptophan operon. Corynebacterium glutamicum Genomic DNA of trp operon of prepibacteriumn Iatophelmrentamn. unidentified gDNA encoding tryptophan synthase. Corynebacterium glutamnicumn Brevibacterium lactofermentumn tryptophan operon. Corynebactenium glutamicum DNA sequence of tryptophan operon. Corynebactenium glutamicumn Brevibactenium lactofermentumn tryptophan operon. Corynebacteriumn glutamicum DNA sequence of tryptophan operon. Corynebacteriumn glutamicumn Genomic DNA of trp operon of prepibacterium Iatophelmentamn. unidentified Brevibacterium lactofermentum tryptophan operon. Corynebacterium glutamicum.

DNA sequence of tryptophan operon. Corynebactenium glutamicumn Genomic DNA of trp operon of prepibacterium Iatophelmentamn. unidentified Corynebacterium glutamicum hom-thrB genes for homoserine dehydrogenase Coiynebacterium and homosenine kinase. glutamicumn Sequence 1 from Patent WO 8809819. Unknown.

DNA encoding for homoserine dehydrogenase(HDH)and homoserine Corynebacterium kinase(HK). glutamicum gDNA encoding diaminopimelate decarboxylase (DDC) and arginyl-tRNA Corynebacterium synthase. glutamnicum Sequence 15 from patent US 5804414. Unknown.

DNA encoding Brevibacterium diaminopimelic acid decarboxylase and arginyl- Corynebacterium tRNA synthase. glutamicum Gallus gallus partial mRNA for ATP-citrate lyase (ACL gene). Gallus gallus Genomic sequence for Arabid apsis thaliana BAC Fl 504 from chromosome 1, Arabidopsis thaliana complete sequence.

Arabidopsis thaliana genome survey sequence T7 end of BAC F14D7 of IGF Arabidopsis thaliana library from strain Columbia of Arabldopsls thaliana, genomic survey sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 108/162. Mycobacterium tuberculosis S coelicolor valS. fpgs, ndk genes. Streptomyces coelicolor Mycobacterium tuberculosis H37Rv complete genome, segment 1081162. Mycobacterium tuberculosis 98,372 98,242 98,949 99,107 98,945 99,165 98.927 98.867 98,792 98,792 98,658 99,905 99,810 97.524 99,931 99,931 99.931 37,538 37,600 41.264 40.773 58.119 38,167 10-Feb-99 29-Sep-97 08-OCT- 1997 (Rel.

52, Created) 10-Feb-99 29-Sep-97 10-Feb-99 29-Sep-97 29-Sep-97 10-Feb-99 29-Sep-97 29-Sep.97 1 2-Sep-93 02-DEC- 1994 29-Sep-97 28-Jul-99 29-Sep-99 28-Jul-99 28-Sep-99 04-OCT- 1999 28-Jun-99 17-Jun-98 03-MAR- 1998 17-Jun-98 rxa00989 1644 GB-BAI :MTVOO8 GBBA1 :SCVALSFP GBBA M1TVOO8 rxa00997 705 GB.6BA2:CGU31225 1817 U31225 Table 4 (continued) Corynebactenumn glutamicum L-proline:NADP+ 5-o .xidoreductase (proC) gene, Corynebactenium complete cds. glutamicum Caenortiabditis elegans chromosome IV clone Y39C12, SEQUENCING IN Coenorhabditis elegans 40,841 2-Aug-96 GB-HTGI :CEY39C12 282838 GBjN1 CEBOQ0l 39416 =xa1l19 1110 GBHTG2:AC005052 144734 GBYTG2AC005052 144734 GB GSS9AQ171808 512 rxa0lO26 1782 GB,.BA1:SC1C2 42210 GBBA1 ATLEUCD 2982 GBBA1:MTVO12 70287 rxaOl 027 1131 GBBA1 :MLCB637 44882 GBBAI :MTCY349 43523 GBBA1:SPUNGMUT 1172 x rxaOlO73 954 GB.BA1:BACOUTB 1004 GB-PR4:AC007938 167237 GBPL2ATAC006282 92577 ncaOlO79 2226 GBBA2AF1 12535 4363 GBBA1:CANRDFGE 6054

N

GB-BA1 :MTVO1 2 70287 rxa0lOBO 567 GBBA2:AF1 12535 4363 GBBA1:CANRDFGE 6054

N

GBBA1 :STNRD 4894 rxaOIO87 999 GB-IN2:AF063412 1093 GByPR3:HS24M1 5 134539 GBJIN2ARU85702 1240 AL009026 Z69634 AC005052 AC005052 AQ171808 AL031 124 X84647 AL021 287 Z99263 Z8301B Z21 702 M15811 AC007938 AC006282 AF1 12535 Y09572 AL021 287 AF112535 Y09572 X73226 AF063412 Z94055 U85702 PROGRESS in unordered pieces.

Caenorthabditis elegans cosmid 630001, complete sequence.

Homo sapiens clone RG038K21, -SEQUENCING IN PROGRESS ~,3 unordered pieces.

Homo sapiens clone RG038K21, SEQUENCING IN PROGRESS ,3 unordered pieces.

HS..3179..Al -G03 T7 CIT Approved Human Genomic Sperm Library D Homo sapiens genomic dlone Plate=3179 Col=5 Row=M, genomic survey sequence.

Streptomyces coelicolor cosmid I1C2.

A.teichomyceticus leuC and leuD) genes.

Mycobacterium tuberculosis H37Rv complete genome; segment 1321162.

Mycobacterium leprae cosmid B637.

Mycobacterium tuberculosis H37Rv complete genome; segment 131/162.

S.pneumoniae ung gene and mutX genes encoding uracil-ONA glycosylase and 8-oxodGTP nucleoside tniphosphatase.

Bacillus subtilis out13 gene encoding a sporulation protein, complete cds.

Homo sapiens done UWGC:djs2Ol from 7q31, complete sequence.

Arabidopsis thaliana chromosome 11 BAC F1 31<3 genornic sequence, complete sequence.

Corynebacteriumn glutamicum putative glutaredoxin NrdH (nrdH), Nrdl (nrdl), and ribonudeotide reductase alpha-chain (nidE) genes, complete cds.

Corynebacterium ammoniagenes nrdH, nrdl, nrdE, nrdF genes.

Mycobacterium tuberculosis H37Rv complete genome; segment 132/162.

Corynebacterium glutamicum putativi; glutaredoxin NrdH (nrdH), Nrdl (nrdl), and nibonucleotide red uctase alpha-chain (nrdE) genes, complete cds.

Corynebactenium ammonlagenes nrdH, nrdl, nrdE, nrdF genes.

S.typhimurium nrdEF operon.

Limnadia lenlicularis elongation factor 1-alpha mRNA, partial cds.

Human DNA sequence from PAC 24M15 on chromosome 1. Contains tenascin-R (restrictin), EST.

Anathix ralla elongation factor-i alpha (EF-la) gene, partial cds.

Haoo apie an Homo sapiens Homo sapiens Streptomyces coelicolor Actinoplanes teichomyceticus Mycobactenumr tuberculosis Mycobactenumr leprae Mycobactedriu tuberculosis Streptococcus pneumoniae Bacillus subtllis Homo sapiens Arabidopsis thaliana Corynebacteriurn glutamnicum Corynebacterium ammoniagenes Mycobacterium tuberculosis Corynebacteriumn glutamicum Corynebactenium ammoniagenes Salmonella typhlmuriumn Limnadia lenticularis Homo sapiens Anathix ralla 36,416 36,416 39,172 39,172 34,661 68,275 65.935 40,454 38,636 51,989 38,088 53,723 34,322 36,181 99,820 75,966 38,296 100,000 65,511 52,477 43,750 37,475 37,319 26-OC T- 1999 2-Sep-99 12-Jun-98 12-Jun-98 17-OCT- 1998 15-Jan-99 04-OCT- 1995 23-Jun-99 17-Sep-97 17-Jun-98 15-Jun-94 26-Apr-93 1-Jul-99 13-MAR- 1999 5-Aug-99 18-Apr-98 23-Jun-99 5-Aug-99 1 8-Apr-98 03-MR- 1997 294g AR- 1999.

23-Nov-99 16-Jul-97 0 00

AI

rxa01095 857 GBBA1:MTCYO1B2 GB-HTG5AC01 1632 1632 rxa0lO97 477 GBBA2:AF030405 GB.BA2:AF030405 rxa0l 098 897 GB-BA2:AF030405 GB.BA1 :MSGY223 GIBBA1:MLCB161O rxa01l100 861 GBBA2:AF051846 35938 175917 175917 774 774 774 42061 40055 738 rxa0ll0l 756 rxa~ll104 729 rxa0 1105 1221 rxa01 106 1449 GB-BA2:AF060558 636 GBJ-ITGI:HSDJ140A 221755 9 GBBA2:AF060558 636 GB-BA1:SC4G6 36917 GB-BAI:STMHISOPA 3981 GB..BA1 :STMHISOPA 3981 GB-BA1:SC4G6 36917 GB-BA1:MTCY336 32437 GB-BA1:MTCY336 32437 GB.,BAM5GY223 42061 GB-BA1:MLCB1 610 40055 GBBAI:MSGY223 42061 Z95554 AC01 1632 AC0l 1632 AF030405 AF030405 AF030405 AD000019 AL049913 AF051 846 AF060558 AL109917 AF060558 AL096884 M31628 M31628 AL096884 Z95586 Z95586 AD000019 AL04991 3 AD00001 9 Table 4 (continued) Mycobacterium tuberculosis H37Rv complete genome; segment 72/162.

Homo sapiens clone RP1 1-3N13. WORKING DRAFT SEQUENCE, 9 unordered pieces.

Homo sapiens clone RP1 1-3N1 3. WORKING DRAFT SEQUENCE. 9 unordered pieces.

Corynebacteriun' glutamicum cyclase (hisF) genve, complete cds.

Corynebacterium glutamicum cyclase (hisF) gene, complete cds.

Corynebacterium glutamicum cyclase (hisF) gene, complete cds.

Mycobacterium tuberculosis sequence from clone y 223 Mycobacterium leprae cosmid B1610.

Corynebactenium glutamicum phosphoribosyformimino-5-a'ilphosphodbosyl-4- imidazolecarboxamide isomerase (hisA) gene, complete cds.

Corynebacterium glutamicum glutamine amidotransferase (hisH) gene, complete ods.

Homo sapiens chromosome 1 clone RP1-140A9, SEQUENCING IN PROGRESS in unordered pieces.

Corynebacterium glutamicum glutamine amidotransferase (hisH) gene, complete cds.

Streptomyces coelicolor cosmid 4G6.

Scoelicolor histidine biosynthesis operon encoding hisD, partial cds.. and hlsC, hisB, hisH, and hisA genes, complete cds.

S.coelicolor histidine biosynthesis operon encoding hisD, partial cds.. and hisC, hisB, hisH, and hisA genes, complete cds.

Streptomyces coelicolor cosmid 4G6.

Mycobacterium tuberculosis H37Rv complete genome; segment 701162.

Mycobacterlum tuberculosis H37Rv complete genome; segment 70/162.

Mycobacterlum tuberculosis sequence from clone y 223 Mycobacterium leprae cosmid 81610.

Mycobacterium tuberculosis sequence from clone y223.

Mycobacterium tuberculosis Homo sapiens Homo sapiens Corynebacterium glutamicum Corynebactenium glutamicum Corynebactenium glutamicum Mycobacterium tuberculosis Mycobacterium leprae Corynebactedrlu glutamicum Corynebacterium glutamicum Homo sapiens Corynebacterium glutamicum Streptomyces coelicolor A3(2) Streptomyces coelicolor Streptomyces coelicolor Streptomyces coelicolor A3(2) Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacterium leprae Mycobacterium tuberculosis 43,243 36,471 36,836 1100.000 41,206 97.933 40,972 61,366 97,154 95,455 30,523 94,462 38,378 60,053 58,333 39,045 60.364 60,931 36,851 60,902 37.233 117-Jun-98 1 9-Nov-99 19-Nov-99 1 3-Nov-97 1 3-Nov-97 13-Nov-97 IlO-DEC- 1996 27-Aug-99 1 2-MAR- 1998 29-Apr-98 23-Nov-99 29-Apr-98 23-Jul-99 26-Apr-93 26-Apr-93 23-Jul-99 24-Jun-99 24-Jun-99 lO-DEC- 1996 27-Aug-99 1996' GB6BA1:MSHISCD 2298 X65542 rxa~ll145 1137 rxaOll62 1449 rxa0l208 846 rxa0l2O9 1528 nca0l2lS 1098 rxa0l 239 2556 rxa0l 253 873 GBlBAI :MTCY336 GB-BA1 :CORAIA GB-BA1 :BRLILVCA GB-PAT:EG8232 GB -PAT:A60299 GByPR3:HS24E5 GB PR3:AC005265 GByHTG2:AC004965 GB-HTG2:AC004965 GByPL2:TAU55859 G8-TG3AC01 1469 GB-ITG3:AC01 1469 GBPLI ABO1 0077 GBBA1:MTCYIOG2 GBIN1 :LEIPRPP GBj-HTG2:HSJ799DI 6 GB-BA1:MTCY48 GBPR2AB029032 GB-GSS9:AQI 07201 GB-PL2:F5O8 GBPL2:F508 GB.JN1 :CELCO6G1 32437 4705 1364 1017 2869 35506 43900 323792 323792 2397 113436 113436 77380 38970 1887 130149 35377 6377 355 99923 99923 31205 Z95586 L09232 D14551 E08232 A60299 Z82185 AC005265 AC004965 AC004965 U55859 AC0l 1469 AC0l 1469 ABO10077 Z92539 M76553 IAL050344 Z74020 AB029032 A0107201 AC005990 AC005990 U41 014 Table 4 (continued) M.smegmatis genes hisD and hisO for histidinol dehydrogenase and histidinol-Mycobacterium smegmatis 60,1111 phosphate aminotransferase, respectively.

Mycobacteriumn tuberculosis H37Rv complete genome; segment 70/162. Mycobacteriumn 58,420 tuberculosis Corynebactenum glutamnicumn acetohydroxy acid synthase (llvB) and (ilvN) Corynebacterium 100,000 genes, and acetohydroxy acid isomeroreductase (ilvC) gene, complete cds. glutamicum Brevibactenium flavumn ilvC gene for acetohydroxy acid isomeroreductase, Corynebactenumr 99,560 complete cds. glutamicum DNA encoding acetohydroxy-acid isomeroreductase. Corynebacterium 99,803 glutamicum Sequence 18 from Patent W09706261. Aspergillus niger 38,675 Human DNA sequence from Fosmid 24E25 on chromosome 22ql1 .2-qter Homo sapiens 36,204 contains parvalbumin, ESTs, STS.

Homo sapiens chromosome 19, cosmid F19750, complete sequence. Homo sapiens 38,363 Homo sapiens clone D.11106H14, -SEQUENCING IN PROGRESS ~,42 H-omo sapiens 36.058 unordered pieces.

Homo sapiens clone OJi 106H14, -SEQUENCING IN PROGRESS ~,42 Homo sapiens 36,058 unordered pieces.

Tritlc4Jm aesttvum heat shock protein 80 mRNA, complete ods- Triticumn aestivum 37,269 Homo sapiens chromosome 19 clone CIT.HSPC.,A75D323, SEQUENCING Homo sapiens 40.000 IN PROGRESS 31 unordered pieces.

Homo sapiens chromosome 19 clone CIT-HSPC_475D23, SEQUENCING Homo sapiens 40,000 IN PROGRESS 31 unordered pieces.

Arabidopsis thaliana genomic DNA, chromosome 5, P1 clone: MYHig, Arabidopsis thaliana 36,803 complete sequence.

Mycobacteriumn tuberculosis H37Rv complete genome; segment 47/162;* Mycobacterium 37,047 tuberculosis Leishmania donovani phosphonibosylpyrophosphate synthetase gene, Leishmanla donovani 50,738 complete cds.

Homo sapiens chromosome I clone RP4-799D16 map p34.3-36.1, Homo sapiens 38,135 SEQUENCING IN PROGRESS in unordered pieces.

Mycobactedriu tuberculosis H37Rv complete genome; segment 69/1 62. Mycobacterium 38,139 tuberculosis Homo sapiens mRNA for KtAA1 109 protein, partial cds. Homo sapiens 39,394 H5 3098_A1_CO3J7 CIT Approved Human Genomic Sperm Library D Homo sapiens 41,408 Homo sapiens genomic clone Plate-3098 Col=5 Row=E, genomic survey sequence.

Arabidopsis thaliana chromosome 1 SAC F508 sequence, complete Arabidopsis thaliana 36.118 sequence.

Arabidopsis thaliana chromosome 1 BAC F508 sequence, complete Arabidopsis thaliana 35,574 30-Jun-93 24-Jun-99 23-Feb-95 3-Feb-99 29-Sep-97 06-MAR- 1998 23-Nov-99 6-Jul-98 12-Jun-98 12-Jun-98 1 -Feb-99 07-OCT- 1999 07-OCT- 1999 20-Nov-99 17-Jun-98 7-Jun-93 29-Nov-99 17-Jun-98 4-Aug-99 28-Aug-98 23-DEC- 1998 30-Nov-95 sequence.

Caenortrabditis elegans cosmid CO6G1.

Caenortrabditls elegans 38,560 rxa01321 1044 GB-GSS14AQ51884 441 3 GB-HTG2'AC007473 194859 AQ5118843 AC007473 Table 4 (continued) HS_51O6-A1D1O..SP6E RPCI-1 1 Human Male BAC Library Homo sapiens Homo sapiens genomnic clone Plate=682 CoI=1 9 Row--G, genomic survey sequence.

Drosophila melanogaster chromosome 2 clone BACR38D12 (10590) RPCI-98 Drosophila melanogaster 38.D.12 map 48A-488 strain y; cn bw sp, -SEQUENCING IN PROGRESS 60 unordered pieces.

GB -TG4AC0111696 115847 AC011696 rxa0l 352 706 GB PL2'ATAC005I 67 83260 Gb-PL2:ATACOOS825 97380 GByTG3:ACOI 1150 127222 rxa0l 360 259 GBE5T32:A1725583 728 GB _yR2:HS227P17 82951 GBEST34:AV171099 173 rxa0l36l 629 GB -RO:AB08915S1 530 GB-EST22:A050532 293 AC0051 67 AC005825 AC0I 1150 A1725583 Z81007 AV171099 ABO0891 5 A1050532 AB008895 AB005237 AQ766840 AL022004 X96471* AL03 1107 AC004054 X96471 A0769223 Drosophila mnelanogaster chromosome 2 clone BACR35FO1 (01 156) RPCI-98 35.F.1 map 48A-48C strain y; cn bw sp, SEQUENCING IN PROGRESS 108 unordered pieces.

Arabidopsis thaliana chromosome 11 BAC F12A24 genomic sequence, complete sequence.

Arabidopsis thaliana chromosome It BAC T24121 genomic sequence, complete sequence.

Homo sapiens clone 4_K-17, LOW-PASS SEQUENCE SAMPLING.

BNLGHi12371 Six-day Cotton fiber Gossypium hirsutum cONA 5' similar to (U86081) root hair defective 3 [Arabidopsis thalianal, mRNA sequence.

Human DNA sequence from PAC 227P17, between markers DXS6791 andDXS8O38 on chromosome X contains CpG island, EST.

AV171099 Mus musculus head C57BLJ6J 14, 17 day embryo Mus muscauius cONA dlone 3200002MI 1, mRNA sequence.

Mus musculus mGpll gene, exon 1.

uc83dlO.yl Sugano mouse kidney mkia Mus musculus cDNA clone IMAGE:1432243 5' similar to TR:035120 035120 MGPI1 P. mRNA seq uence.

Mus muscailus mRNA for mGpil p. complete cds.

Arabidopsis thaliana genonhic DNA, chromosome 5, PI clone: MJJ3,compiete sequence.

HS..2026_A2_C09_T7C CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=2026 Col=18 Row=E, genomic survey sequence.

Mycobacterium tuberculosis H37Rv complete genome: segment 40/162.

C.glutamicum lysE and lysG genes.

Streptomyces coelicolor cosmid 5A7.

Homo sapiens chromosome 4 clone B220G8 map 4q21, complete sequence.

C.glutamicum lysE and lysG genes.

HS_3155B2-G10jT7C CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=3155 Col=20 Row=-N, genomic survey sequence.

Arabiopia thlanase Arabidopsis thaliana Homo sapiens Gossypium hirsutumn Homo sapiens Mus musculus Mus musculus Mus musculus Mus musculus Arabidopsis thaliana Homo sapiens Mycobacterium tuberculosis Corynebacterium glutamicum Streptomyces coelicolor Homo sapiens Corynebacterum glutamicum Homo sapiens 41,121 40,634 38.290 34.311 34,311 37.722 38,492 39,738 46,237 45,574 44,097 4 1,316 36,606 37,916 37,419 34,831 35,138 37.277 100.000 38,400 1999 2-Aug-99 26-OCT- 1999 1998 12-Apr-99 01-OC- 1999 11-Jun-99 23-Nov-99 6-Jul-99 28-Sep-99 9-Jul-98 23-Nov-97 20-Nov-99 28-Jul-99 24-Jun-99 24-Feb-97 27-Jul-98 9-Jul-98 24-Feb-97 28-Jul-99 GB,,30:AB008895 rxa0l 381 944 GB._PL1:AB005237 GBGSS5:AQ766640 rxa01393 993 rxa0l 394 822 GBBA1 :MTV043 GB,_BA1 :CGLYSEG GB,,BA1 :SCSA7 GByR3:AC004054 GB-BiAl:CGLYSEG GBG555:AQ769223 3062 87835 491 68848 2374 40337 112184 2374 500 GB, BAI :CGLYSEG 2374 X96471 rxa0l416 630 GBBA1:SC3C3 31382 GB-BA1:MLCB22 40281 GB,,BA1:MTV0O2 56414 rxa01442 1347 GB-BA1:D90827 18886 GB-BAI:D90828 14590 GBBA2:AE000279 10855 rxa01446 1413 GBBA1:SCH1O 39524 GB-BA1 :MTY13EIO 35019 GB BA1:MLCB4 36310 rxa01483 1395 GB-BAI:MTCY98 31225 GBBA1:MSGB1229C 30670

S

GB-BA2AF027507 5168 rxa01486 757 GB-BA1:MTV0O2 56414 GB BA1:MLCB22 40281 GB.BA1:SC3C3 31382 rxa01489 1146 GB-BA1:CORFADS 1547 GB-BA1 :MLCB22 40281 GB-BA1:SC1OA7 39739 AL031231 Z98741 AL008967 D90827 D90828 AE000279 AL049754 Z95324 AL023514 Z83860 L78812 AF027507 AL008967 Z98741 AL031231.

D37967 Z98741 AL07861 8 AL008967 AA356956 X92380 Z93380 Z93380 AL049641 U88875 Table 4 (continued) C.glutamicum lysE and lysG genes.

Streptomyces coelicolor cosmid 3C3.

Mycobacterium leprae cosmid 822.

Mycobacterium tuberculosis H37Rv complete genome; segment 1221162.

Ecoli genomic DNA, Kohara clone #336(41.2-41.6 min.).

Ecoli genomic DNA, Kohara clone #336gap(41.6-41.9 min.).

Escherichia coi K-12 MG1655 section 169 of 400 of the complete genome.

Streptomyces coelicolor cosmid H-I10.

Mycobacterium tuberculosis H37Rv complete genome; segment 18/162.

Mycobacterium ieprae cosmid B4.

Mycobacterium tuberculosis H37Rv complete genome; segmen~t 103/162.

Mycobacterium leprae cosmid B1 229 DNA sequence.

Mycobacterium smegmatis dGTPase (dgt), and primase (dnaG) genes, complete cds; tRNA-Asn gene, complete sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 122/162.

Mycobacterium leprae cosmid B22.

Streptomyces coelicolor cosmid 3C3.

Corynebacterium ammoniagenes, gene for FAD synthetase, complete cds.

Mycobacterium leprae cosmid B22.

Streptomyces coelicolor cosmid IGAT.

Mycobacterium tuberculosis H37Rv complete genome; segment 1221162.

EST65614 Jurkat T-celis Ill Homo sapiens cDNA 5Vend, mRNA sequence.

Omossambicus prolactin I gene.

Caenorhabditis elegans cosmid F28C1 2, complete sequence.

Caenorhabditis elegans cosmid F28C12, complete sequence.

Streptomyces coelicolor cosmid E9.

Mycobacterium avium hypoxanthine-guanifle phosphonibosyl transferase gene, complete cds.

Corynebacterium 33.665 giutamicum Streptomyces coelicolor 62,726 Mycobacterium ieprae 39,159 Mycobacterium 37,340 tuberculosis Escierichia coli 58,517 Escherichia coi 56,151 Escherichia coli 56,021 Streptomyces coelicolor 39,037 Mycobacterium 40,130 tuberculosis Mycobacterium leprae 37,752 Mycobacterium 39,057 tuberculosis Mycobacterium leprae 54,382 Mycobacterium smegmatis 52,941 Mycobacterium 40,941 tuberculosis Mycobacterium leprae 38,451 Streptomyces coelicolor 61,194 Corynebactenium 58,021 ammoniagenes Mycobacterfumn ieprae 38.414 Streptomyces; coelicolor 36,930 Mycobacterium 37,062 tuberculosis Homo sapiens 37.647 Tilapia mossambica 38,289 Caenvorthabdilis elegans 37,984 Caenvorhabditis elegans 38,469 Strepto myces coelicolor 39,021 Mycobacterium avium 57.521 24-Feb-97 1 0-Aug-98 -Aug-9 17-Jun-98 21-MAR- 1997 21-MAR- 1997 12-Nov-98 04-MAY- 1999 1 7-Jun-98 27-Aug-99 1 7-un-98 1 5-Jun-96 1 6-Jan-98 17-Jun-9 wA 22-Aug-97 10O-Aug-98 8-Feb-99 22-Aug-97 9-Jun-99 17-Jun-98 21 -Apr-97

P

19-OCT- 1995 23-Nov-98 19-MY- 21999

OS-MAR-

1997 rxaOl49l 774 rxa01508 1662 ncaO1512 723 GB-BAI:MTV0O2 56414 GB-EST13:AA356956 255 GB,.OV:OMDNAPROI 7327 GBjN1:CEF28C12 14653 GB-INI :CEF28C12 14653 GBBA1:SCE9 37730 GB SAl :MAU88875 840 GB...BA1:MVTY15C1O rxa0l514 711 G6._BAIMTCY7H-7B GB BAi :MLCB2548 GB-PL1 :EGGTPCHI rxa0l5l5 975 GB BA1:ECOUW93 GB BA1 :ECOUW93 GB-BA1:MTCY49 rxa0l5l6 513 GB-IN1:DME238847 GB-HTG3:AC00921 0 GB IN2:AF132179 rxa01 517 600 GB PL2:F6H8 GB,.PL2:AF038831 GB _PL2:ATAC005957 rxa0l52l 921 GB-BA1:ANANIFBH GB...PR2AC002461 GB PR2:AC002461 ncaol 528 651 GB RO:MM437P9 GByPR3:AC005740 GB-PR3:AC005740 rxa01551 1998 GB-BA1:MTCY22GIO GB-BA2:ECOUW89 GB BA1 :SCQ1 1 rxa0lS6l 1053 GB-IN1:CEY62H9A GByPR4:HSU51003 GBOM:PIGDAO1 rxa0lS99 1785 GB-BAI:MTC1125 GB.BA1 :U00021 33050 24244 38916 242 338534 338534 39430 5419 103814 4842 82596 647 108355 5936 197273 197273 165901 186780 186780 35420 176195 15441 47396 3202 395 37432 39193 Z95436 Z95557 AL023093 Z49757 U1 4003 U14003 Z73966 AJ238847 AC00921 0 AF1 321 79 AF1 78045 AF038831 AC005957 J051 11 AC002461 AC002461 AL049866 AC005740 AC005740 Z84724 U00006 AL096823 AL032630 U51 003 M1 8444 Z98268 U00021 Table 4 (continued) Mycobacterlum tuberculosis H37Rv complete genome: segment 1541162. Mycobacterium tuberculosis Mycobacterium tuberculosis H37Rv complete genome; segment 153/162. Mycobacterium tuberculosis Mycobacterium leprae cosmid B2548. Mycobacterium Ieprae E.gracllls mRNA for GTP cyclohydrolase I (core region). Euglena gracilis Escherlchia coi K-12 chromosomal region from 92.8 to 00.1 minutes. Escherichia coli Esclierichia coll K-12 chromosomal region from 92.8 to 00.1 minutes. Escherichia coi Mycobacteriumn tuberculosis H37Rv complete genome; segment 93Y162. Mycobacterium tuberculosis Drosophila melanogaster mRNA for drosophila dodeca-satellite protein 1 Drosophila melanogaster (IDDP.-1).

Drosophila melanogaster chromosome 2 clone BACRO1 106 (D1054) RPCI-98 Drosophila melanogaster 01.1.6 map 5513-55D strain y; cri bw sp, SEQUENCING IN PROGRESS 86 unordered pieces.

Drosophila melanogaster clone 1D21677 unknown mRNA. Drosophila melanogaster Arabldopsis thaliana BAC F6H8. Arabidopsis thaliana Sorosporium saponarlae internal transcribed spacer 1, 5.8S ribosomal RNA Sorosporium saponaniae gene; and internal transcribed spacer 2, complete sequence.

Arabidopsis thallana chromosome 11 BAC T15.114 genomlc sequence, Arabidopsis thaliana complete sequence.

Ariabaetia sp. (clone AnH2O.1) nitrogen fixation operon nifB, fdxN, nifS, nifU, Anabaena sp.

and nlfH genes, complete cds.

Human SAC clone RG204116 from 7q31, complete sequence. Homo sapiens Human BAC clone RG204116 from 7q31, complete sequence. Homo sapiens Mus musculus chromosome X, clone 437P9. Mus musculus Homo sapiens chromosome 5p, BAC clone 50g21 (LBNL HI 54), complete Homo sapiens sequence.

Homo sapiens chromosome 5p, BAC clone 50g2l (LBNL H 154), complete Homo sapiens sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 21/162. Mycobacterium tuberculosis E. coi chromosomal region from 89.2 to 92.8 minutes. Escherichia coli Streptommyces coelicolor cosmid 011. Streptomyces coelicolor Caenortiabditis elegans cosmid Y62H-9A, complete sequence. Caenortiabditis elegans Homo sapiens DLX-2 (DLX-2) gene, complete cds. Homo sapiens Pig 0-amino acid oxidase (DAO) gene, exon 1. Sus scrofa Mycobacterium tuberculosis H37Rv complete genome; segment 76/162. Mycobactenumr tuberculosis Mycobacteriurn leprae cosmld L247. Mycobacterium leprae 40,086 43,343 38,177 64,876 38,943 37,500 38,010 36,346 37,897 36,149 35,846 40.566 38.095 38,206 36,623 34,719 37,500 37,031 38,035 38,371 38.064 60,775 38.514 37,730 39.340 63,300 36.756 17-Jun-98 1 8-Jun-98 27-Aug-99 1995 1 7-Apr-96 1 7-Apr-96 24-Jun-99 1 3-Aug-99 20-Aug-99 3-Jun-99 19-Aug-99 13-Apr-99 7-Jan-99 26-Apr-93 20-Aug-97 20-Aug-97 29-Jun-99 Ol-OCT- 1998 Ol-OCT- 1998 17-Jun-98 I 7-DEC- 1993 8-Jul-99 2-Sep-99 07-DEC- 1999 27-Apr-93 17-Jun-98 29-Sep-94 GB BA1 :MLCB1 351 rxa01617 795 GBR2:HSMTMO GBPR2:HSI 3D10 GB...PR2:HSMTMO rxa01657 723 GBBA1:MTCYI10 GB..EST6:D79278 GB 8A2.AF129925 rxa01660 675 GB..BA1:MTVO1 3 GB RO:MMFVI GBPAT:A67508 rxa01678 651 GB-VI:1VU95309 GBYI:TVU95303 GBYI:TVU95302 rxa01679 1359 GB-EST5:H91843 38936 Z95117 217657 AL034384 153147 217657 25949 392 10243 11364 6480 6480 600 600 600 362 362 1202 42210 493 1020 1034 3085 3085 4115 AL021407 AL034384 Z95387 D79278 AF1 29925 AL021 309 X97719 A67508 U95309 U95303 U95302 H91843 G26925 AF139451 AL031 124 A1064232 AF1 17896 AF0671 23 M37227 X1 3804 AF1 24600 Table 4 (continued) Mycobacterium leprae cosmid 81 351.

Human chromosome Xq28, cosmid clones 7H3, 14D7, C1 230, 1 1E7, F1096.

A12197, 12G8, A09100; complete sequence bases 1. .217657.

Homo sapiens DNA sequence from PAC 13010 on chromosome 6p22.3-23.

Contains CpG Island.

Human chromosome Xq28, cosmid clones 7H3. 14D7, C1 230, 11 E7, F1 096.

A1 2197, 12G8. A091 00; complete sequence bases 1. .217657.

Mycobacteriumn tuberculosis H37Rv complete genome; segment 117/1 62.

HUM213D06B Human aorta polyA+ (TFujtwara) Homo sapiens cONA clone GEN-21 3006 mRNA sequence.

Thiobacilius ferrooxidans carboxysome operon. complete cds.

Mycobacterium tuberculosis H37Rv complete genome: segment 134/162.

M.musculus retrovirus restriction gene FyI.

Sequence 1 from Patent W0974341 0.

Tula virus 064 nudleocapsid protein gene, partial cds.

Tula virus 052 nucleocapsid protein gene, partial cds.

Tula virus 024 nucleocapsld protein gene, partial cds.

ys8leOI.sl Soares retina N2b4HR Homo sapiens cDNA clone IMAGE:221208 3' similar to gb:X63749..mal GUANINE NUCLEOTIDE- BINDING PROTEIN GMT, ALPI-IA-1 (HUMAN);, mRNA sequence.

human STS SHGC-30023, sequence tagged site.

Gossypium robinsoni CeIA2 pseudogene, partial sequence.

Streptomyces coelicolor cosmid 1C2.

GH04563.5prime GH Drosophila melanogaster head pOT2 Drosophila melanogaster cDNA clone GH04563 5prime, mRNA sequence.

Drosophila melanogaster neuropeptide F (npf) gene, complete cds.

Lactobacillus reuteri cobalamin biosynthesIs protein J (cbIJ) gene, partial cds; and uroporphyrin-Ill C-methyltransferase (sumT) gene, complete cds.

Rat heavy neurofliament (NF-H) polypeptide, partial cds.

Rat mRNA for heavy neurofilament polypeptide NF-H C-terminus.

Corynebacterlum giutamicum chorismate synthase (aroC), shikimate klnase (aroK), and 3-dehydroquinate synthase (aroB) genes, complete cds; and putative cytoplasmic peptidase (pepO) gene, partial cds.

Mycobacterlum tuberculosis H37Rv complete genome: segment 1111162.

Mycobacteriumn ieprae Homo sapiens Homo sapiens Homo sapiens Mycobacterium tuberculosis Homo sapiens Thiobacillus ferrooxidans Mycobacterium tuberculosis Mus musculus Mus musculus Tula virus Tula virus Tula virus Homo sapiens Homo sapiens Gossypium robinsonli Streptomyces coelicolor Drosophilla melanogaster Drosophila melanogaster Lactobacillus reuteri Rattus norveglcus Rattus sp.

Corynebacterium giutamicum Mycobacterium tuberculosis 36,756 40,811 38,768 39,018 40,656 44.262 40,709 40,986 35,364 35.364 41.894 41 .712 39,576 39,157 24-Jun-97 5-Jul-99 23-Nov-99 5-Jul-99 1 7-Jun-98 9-Feb-96 17-MAY- 1999 17-Jun-98 29-Aug-96 1999 28-OCT- 1997 28-OCT- 1997 28-OCT- 1997 29-Nov-95 0 0 0 0 00 rxa0l 690 1224 rxa01692 873 rxa01698 1353 GBS§TS:G26925 GByLZ:AF1 39451 GB BAI :SC1 C2 GB..EST22:A1064232 GB IN2:AF117896 GB-BA2AF671 23 GB..RO:RAThFHPEF GB RO:RSNFH GB7BA2AF1 24600 39,157 14-Jun-96 38,910 1-Jun-99 60,644 15-Janv-99 38,037 24-Nov-98 36,122 2-Jul-99 48,079 3-Jun-98 37,093 27-Apr-93 37.093 14-Jul-95 100,000 04-MAY- 1999..

36,323 17-Jun-198 GB-BA1:MTCY1 59 33818 Z83863 Table 4 (continued) Mycobacterium Ieprae cosmid B937 DNA sequence.

rxa01699 693 GB._BA1:MSGB937C 38914 178820

S

GB...BA2:AF124600 4115 AF124600 GB-BA2:AF016585 41097 AF016585 nca01712 805 rxa0l7l9 684 rxa01720 1332 rxa0l 746 876 rxa01747 1167 rxa0l 757 924 Mycobacterium Ieprae GBEST9:C19712 399 GB EST2I:MA952466 278 GBEST211:AA952466 278 GBI-HTG1:HSDJ534K 154416 7 GBHTGI:HSDJ534K 154416 7 GBEST27A1447108 431 GBR4AC006322 179640 GBP-2:TM018A1O 106184 GByPR4:AC006322 179640 GB-EST3:R46227 443 GB-EST3:R46227 443 C1 9712 AA952466 AA952466 AL1 09925 AL1 09925 A1447108 AC006322 AF01 3294 AC006322 R46227 R46227 Z70283 Z98741 AL096872 AA91 8454 H34042 AA899038 Carynebacterium giutamicum chorismate synthase (aroC), shikimate kinase Corynebactenumr (arok), and 3-dehydroquinate synthase (aroB) genes, complete cds; and glutamicum putative cytoplasmic peptidase (pepO) gene, partial cds.

Streptomyces caelestis cytoclhrome P-450 hydroxylase homolog (nidi) gene, Streptomyces caelestls partial ods: polyketidq synthase modules 1 through 7 (nidA) genes, complete ods; and N-methyltransferase homolog gene, partial cds.

C19712 Rice panicle at ripening stage Oryza sativa cDNA clone E10821_lA, Oryza sativa mRNA sequence.

TENS1404 T. cruzi epimastigote normalized cONA Library Trypanosoma cruzi Trypanosoma cruzi cDNA clone 1404 mRNA sequence.

TENS14O4 T. cruzi epimastigote normalized cDNA Library Trypanosoma cruzi Trypanosoma cruzi cDNA clone 1404 mRNA sequence.

Homo sapiens chromosome 1 clone RP4-534K7, *SEQUENCING IN Homo sapiens PROGRESS in unordered pieces.

Homo sapiens chromosome 1 clone RP4-534K7, SEQUENCING IN Homo sapiens PROGRESS in unordered pieces.

mq9leOB.xl1 Stratagene mouse heart (#937316) Mus musculuscDNA clone Mus musculus IMAGE:58611 183', mRNA sequence.

Homo sapiens PAC clone DJ11060131 from 7q1 1.23-q21.1, complete Homo sapiens sequence.

Arabidopsis thaliana BAC TMO18A1O. Arabidopsis thaliana Homo sapiens PAC clone DJ1060B11I from 7q1 1.23-q21.1, complete Homo sapiens sequence.

yg52a03.sl Soares infant brain I NIB Homo sapiens cDNA clone Homo sapiens IMAGE:36000 mRNA sequence.

yg52a03.sl Soares infant brain 1 NIB Homo sapiens cDNA clone Homo sapiens IMAGE:36000 mRNA sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 98/162. Mycobacterium tuberculosis Mycobacter leprae cosmid B22. Myoobacterium leprae Streptonmyces coelicolor cosmid 5F7. Streptomyces coelicolor A3(2) om38c02.sl Soares NFL T -GBC..S1 H-omo sapiens cDNA clone Homo sapiens IMAGE:1 5432983' similar to WP:F28F8.3 CE09757 SMALL NUCLEAR RIBONUCLEOPROTEIN E mRNA sequence.

EST1 10563 Rat PC-1 2 cells, NGF-treated (9 days) Rattus sp. cONA clone Rattus sp.

RPNBI81 5'end, mRNA sequence.

NCP6G8T7 Penthecial Neurospora crassa cONA clone NP6G8 3'end, mRNA Neurospora crassa sequence.

62,780 15-Jun-96 100.000 04-MAY- 1999 40,260 07-DEC- 1997 45,425 24-OCT- 1996 40,876 29-OCT- 1998 41,367 29-OCT- 1998 35,651 23-Nov-99 35.651 23-Nov-99 39,671 09-MAR- 1999 35,817 18-MAR- 1999 35,698 12-Jul-97 37,243 18-MAR- 1999 42,812 22-MLAY- 1995 42,655 22-MAY- 1995 59,294 17-Jun-98 57,584 22-Aug-97 61,810 22-Jul-99 39,655 23-Jun-98 GB.6A1 :MTCY1 90 GBIBA1 :MLCB22 GBBA1:SCSF7 34150 40281 40024 GBEST1:AA918454 416 GB..EST4:H34042 345 GB-EST20:MA899038 450 35,942 40,000 2-Apr-98 1 2-Apr-98 rxa~l8O7 915 GB -BA1:AP000063 GByHTG4:ACO1 0694 GB-HTG4:ACO1 0694 rxa0l82l 401 GB-BA1:CGL007732

GB-RO:RATALGL

GE!OV:APIGY2 rxa0l 835 654 GBIEST3O.A1629479 GB.STS:G48245 GB.GSS3:B49052 rxa0l 850 1470 GB -BA2:ECOUW67-.

0 GBBA2AEOOO392 GB.,BA2:U.3271 5 rxa01878 1002 GBHTGI:CEY64FI I GB I-ITGI:CEY64F11 GB-HTG1 :CEY64F1 1 rxaOl 892 852 GB Al:MTCY274 GB,.BA1:MLCB25O GB._BAI:MSGB211529C

S

rxa0l 894 978 GBBAl :MTCY274 GBjN1:CE LF46H5 GByHTG3AC009204 185300 AP000063 115857 AC010694 115857 AC010694 4460 AJ007732 7601 M24108 1381 X78272 353 A1629479 515 G48245 515 B49052 110000 U18997 10345 AE000392 13136 U32715 177748 Z99776 177748 Z99776 177748 Z99776 39991 Z74024 40603 Z97369 36985 L78824 39991 Z74024 38886 U41543 115633 AC009204 Table 4 (continued) Aeropyrum pemnix genomic DNA, section 617.

Drosophila melanogaster clone RPCI98-6H2. SEQUENCING IN PROGRESS 75 unordered pieces.

Drosophila melanogaster clone RPCI98-6H2,~ SEQUENCING IN PROGRESS 75 unordered pieces.

Corynebacterium glutamicum 3 ppc gene, secG gene, amt gene, ocd gen~e and 5' soxA gene.

Rattus norvegicus (clone A2U42) alpha2u globulin gene, exons 1-7.

Anas platyrhynchos (Super M) IgY upsilon heavy chain gene, exon 2.

486101D10.xl 486 leaf primordia cONA library from Hake lab Zea mays cDNA, mRNA sequence.

SHGC-62915 Human Homo sapiens STS genomic, sequence tagged site.

RPCI1 1 -4112.TV RPCI-1 1 Homo sapiens genomlc clone RPCI-1 1-4112, genomic survey sequence.

Escherlchla coil K-1 2 chrromosomal region from 67.4 to 76.0 minutes.

Escheridiia coli K-i12 MG1 655 section 282 of 400 of the complete genome.

Haemnophilus Influenzae Rd section 30 of 163 of the complete genome.

Caenorhabditis elegans chromosome IV clone Y64F1 1. -SEQUENCING IN PROGRESS in unordered pieces.

Caenorhabditis elegans; chromosome IV clone Y64F1 1, -SEQUENCING IN PROGRESS in unordered pieces.

Caenorhabditis elegans chromosome IV clone Y64F1 1, SEQUENCING IN PROGRESS in unordered pieces.

Mycobacterium tuberculosis H37Rv complete genome; segment 126/1 62.

Mycobacterium leprae cosmid 8250.

Mycobacterium leprae cosmid B1 529 DNA sequence.

Mycobacterlum tuberculosis H37Rv complete genome; segment 126/162.

Aeropyrum pemix Drosophila melanogaster Drosophila melanogaster Corynebacterium glutamicum Rattus norvegicus Anas platyrtiynchos Zea mays Homo sapiens Homo sapiens Escheichia col Escherichia coi Haemnophilus lnfluenzae Rd Caenorhabditis elegans Caenorhabditis elegans Caenorhabdltis elegans Mycobacterlum tuberculosis Mycobacterium leprae Mycobacterium leprae Mycobactenium tuberculosis 40,067 35,450 35,450 100,000 38,692 36,962 38,109 37,021 37,021 37,196 38,021 39.860 37,564 37.564 37,576 35,910 64,260 64,260 37,229 38,525 31,579 99,733 70.321 22-Jun-99 16-OCT- 1999 16-OCT- 1999 7-Jan-99 1 1994 15-Feb-99 26-Apr-9.9 26-MAR- 1999 8-Apr-99 U18997 1 2-Nov-98 29-MAY- 1998 14-OCT- 1998 14-OCT- 1998 14-OCT- 1998 19-Jun-98 27-Aug-99 15-Jun-96 19-Jun-98 29-Nov-96 18-Aug-99 5-Aug-99 1 8-Apr-98 Caenorhabdits elegans cosmld F46H5. Caenortrabditis elegans Drosophila melanogaster chromosome 2 clone BACRO3E19 (DI 033) RPCI-98 Drosophila melanogaster 03.E.19 map 36E-37C strain y, cn bw sp, SEQUENCING IN PROGRESS 94 unordered pieces.

Corynebacterium glutamicum nibonucleotlde reductase beta-chain (nrdF) Corynebacterumn gene, complete cds. glutamicurn Corynebacterium ammoniagenes nrdH, nrdl, nrdE, nrdF genes. Corynebacterium ammoniagenes rxa0l920 1125 GB..BA2:AF1 12536 1798 GB.,BA1 :CANRDFGE 6054

N

AFI 12536 Y09572 Table 4 (continued) 1228 AF0501 68 Corynebacterium ammoniagenes ribonucleoside diphosphate reductase small Corynebacterlum rxa01928 960 rxa01929 936 rxaOl94O 1059 rxa02022 1230 rxa02024 859 GB-BA2:AFO501 68 0B,6BA1 :CGIPAN GB-PLI AP000423 GB-PL1 AP000423 GB,.BA1:CGPAN GB-BAI:XCU33548 GB-BAI:XANHRPB6

A

GBJIN2:CFU43371 GB-BA2:AE001467 GB ROAF1 75967 GB-BA1 :CGDAPE 2164 154478 154478 2164 8429 1329 1060 11601 3492 1966 X96580 AP000423 AP000423 X96580 U33548 M99174 U43371 AE001467 AFi 75967 X81 379 X85965 U47055 Z93777 L01536 U1 5180 subunit (nrdF) gene, complete cds.

C.glutamicum panB, panC xylB genes.

Arabidopsis thallana chloroplast genomic; DNA, complete sequence.

strain:Columbia.

Arabidopsis thaliana chlorop last genomic DNA, complete sequence.

straln:Columbia.

C.glutamicum pan B. panC xylB genes.

Xanthomonas campestris hrpB pathogenicity locus proteins HrpBl. HrpB2, HrpB3, HrpB4, HrpB5, HrpB6, HrpB7, HrpB8, HrpAl, and ORF62 genes, complete cds.

Xanttiomonas campestris hrpB6 gene, complete cds.

Crithidla fasciculata inosine-uridine preferring nucleoside hydrolase (IUNH) gene, complete cds.

Helicobacter pylori, strain J99 section 28 of 132 of the complete genome.

Homo sapiens Leman coiled-coil protein (LCCP) mRNA, complete cds.

C.glutamicum dapE gene and orf2.

C.glutamlcum ORF3 and aroP gene.

Anabaena PCC7120 nitrogen fixation proteins (niflE, OiN, NIfX, nWV genes, complete cds, and nitrogenase (nifK) and hesA genes, partial cds.

Myocbacterium tuberculosis H371Rv complete genome; segment 521162.

M. leprae genomic dna sequence. cosmid bI 91 2.

Mycobactenum leprae cosmid B 1756.

ammoniagenes Corynebacterium glutamicum Chloroplast Arabidopsis thaliana Chloroplast Arabidopsis thaliana Corynebacterum glutamicum Xanthomonas campestnis pv. vesicatorla Xanthomonas campestnis Crithidia fasciculata Helicobactdr pylori J99 Mus musculus Corynebactedum glutamicum Corynebacterium glutamlcum Anabaena PCC71 20 Mycobacterium tuberculosis Mycobacteium leprae Mycobacterium leprae 100,000 35,917 33,925 100,000 38,749 39,305 61,417 38.560 40,275 100,000 38,889 36,647 59,415 57,093 57,210 11I-MAY- 1999 15-Sep-99 15-Sep-99 11-MiAY- 1999 19-Sep-96 14-Sep-93 18-Jun-96 20-Jan-99 26-Sep-99 8-Aug-95 30-Nov-97 1 7-Feb-96 117-Jun-98 14-Jun-96 09-MAR- 1995 72,082 23-Apr-98 GBiBAI:CGDNAARO 2612

P

GB-.BAI APU47055 6469 GB-BA1:MTC1364 29540 GBBAI :MSGB1 91 2C 38503

S

GB..BAIWMLUI5118O 38675 rxa02027 rxa02O3I rxa02072 1464 GB-BA1:CGGDHA 2037 GB,,.BA1:CGGDH 2037 GB,.,BA:PAE18494 1628 X72855 X59404 Y18494 C.glutamicum GDHA gene.

Corynebactenium glutamicum, gdh gen for glutamate dehydrogenase.

Pseudomonas aeruginosa gdhA gene, strain PACi.

Corynebactenium 99,317 glutamicum Corynebactenium 94,387 glutamicum Pseudomonas aeruginosa 62,247 24-MAY- 1993 30-Jul-99 6-Feb-9 rxa02085 2358 GB SA11:MTCY22G8 22550 GBBAI:MLCB33 42224 GB SAl :ECOUW85 91414 rxa02093 927 GBEST14:M4481146 452 rxa02106 1179 rxaO2ll11 1407 rxa02112 960 rxa02134 1044 rxa02135 1197 GBEST1 7:AA64 1937 GB-PR3:AC003074 GB BA1 :SC1A6 GBPR4:AC005553 GB-EST3:R49746 GB-BA1 :SC6G1 0 GB.BAI :U0001 0 GB-BAI :MTCY336 GB-ITG3.AC010579 GBGSS3:B309839 GBHTG3:AC01 0579 444 143029 37620 179651 397 36734 41171 32437 157658 1191 1 57658 Z95585 Z94723 M87049 AA448146 AA641 937 AC003074 AL023496 AC005 553 R49746 AL049497 U00010 Z95586 AC01 0579 B09839 AC01 0579 X83011 A1731 596 X83011 AL023807 AL022347 Table 4 (continued) Mycobacteriumn tuberculosis H37Rv complete genome; segment 49/162. Mycobacterlumn tuberculosis Mycobactenumn Ieprae cosmid B33. Mycobacteriumn leprae E. coi genomic sequence of the region from 84.5 to 86.5 minutes. Esclierichla coi zw82h0 .r1 Soares testis NHT H-omo sapiens cDNA clone IMAGE:782737 Homo sapiens mRNA sequence.

nsl8blO.rl NCI CGAPGCB1 Homo sapiens cDNA clone IMAGE:1183963 Homo sapiens mRNA sequence.

Human PAC clone DJ0596009 from 7p15, complete sequence. Homo sapiens Streptomyces coelicolor cosmid 1A6. Streptomyces coelicolor Homo sapiens ch~romosome 17, clone hRPK.112...L9, complete sequence. Homo sapiens yg7lglO.rl1 Soares infant brain 1iNIB Homo sapiens cDNA clone Homo sapiens IMAGE:38768 5' similar to gb:V00567 BETA-2-MICROGLOBULIN PRECURSOR (HUMAN);. mRNA sequence.

Streptomyces coelicolor cosmid 6G1 0. Streptomyces coelioolor Mycobactenumn leprae cosmid B1 170. Mycobacteriumn leprae Mycobacterium tuberculosis H37Rv complete genome; segment 70/1 62. Mycobacteriumn tuberculosis Drosophila melanogaster chromosome 3 clone BACRO9DO8 (01 101) RPCI-98 Drosophila melanogaster 09.0.8 map 96F-96F strain y; cn bw sp, -SEQUENCING IN PROGRESS 121 unordered pieces.

T12A12-Sp6 TAMU Arabldopsis thaliana genomic clone T12A1 2, genomic Arabidopsis thaliana survey sequence.

Drosophila melanogaster chromosome 3 clone BACRO90308 (Dl 101) RPCI-98 Drosophila melanogaster 09.D.8 map 96F-96F strain y; cn bw sp, -SEQUENCING IN PROGRESS 121 unordered pieces.

Scoelicolor secY locus DNA. Strcptomyces coelicolor BNILG~I1O0185 Six-day Cotton fiber Gossypiumn hirsutumn cDNA 5' similar to Gossypiumn hirsutumn (AC004005) putative ribosomal protein L7 fArabidopsis thalianal. mRNA sequence.

Scoelicolor secY locus DNA. Streptomyces coelicolor Human DNA sequence from clone RP3-525L-6 on chromosome 6p22.3- 2 3 Homo sapiens Contains CA repeat. STSs. GSSs and a CpG Island, complete sequence.

Arabidopsis thaliana DNA chiromosome 4, BAC clone F21P8 (ESSA project). Arabidopsis thaliana 38,442 56,486 52,127 34.163 35.586 31,917 35,818 34,.274 41.162 50,791 37,563 39,504 37,909 37,843 37,909 36,533 33,451 36.756 34,365 34,325 17-Jun-98 24-Jun-97 29-MAY- 1995 4-Jun-97 27-OCT- 1997 6-Nov-97 13-Jan-99 31 -DEC- 1998 18-MAY- 1995 24-MAR- 1999 01-MAR- 1994 24-Jun-99 24-Sep-99" 114-MAY- 1997 24-Sep-99 02-MAR- 1998 11-Jun-99 02-MAR.

1998 23-Nov-99 9-Jun-99 GB SAl:SCSECYDN 6154

A

GB.EST32:A731 596 568 GB-BA1 :SCSECYDN

A

GBJ'R3:HS525L6 GBJ'L2:ATF21 P8 6154 168111 85785 Arabidopsis thaliana 33,874 ui 98 GB-PL2:U89959 G~yL2:U89959 106973 U89959 Arabidopsis thaliana SAC T7123, complete sequence.Arbdpithiaa 387 6Ji-9 Table 4 (continued) Arabidopsis thaliana chromosome 11 BAC T3A4 genomic sequence, complete Arabidopsis thallana rxa02136 645 GB-PL2:ATAC005819 57752 AC005819 GB _PL:15K<9 GB PL2:U89959 GB-BA1:MTCY1 90 71097 AC005278 106973 U89959 34150 Z70283 sequence.

Arabidopsis thaliana chromosome 1 BAC F15K9 sequence, complete sequence.

Arabidopsis thaliana BAC T7123, complete sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 98/162.

rxa02139 1962 rxaO21 53 903 rxa02154 414 rxaO2Il55 1287 rxa02I56 1074 GB BA1:MSGB1 554C 36548

S

GB BAI :MSGB1 551 C 36548

S

GB.BA2:AF049897 9196 GB-BAIAF005242 1044 GBBA1:CGARGCJB 4355

D

GB-BA2AF049897 9196 GB-BA1 :AF005242 1044 GBBA1 :CGARGCJB 4355

D

GB,,BA1:CGARGCJB 4355

D

GB BA2.AF049897 9196 GB BA1:MSGB1 133C 42106

S

GB-BA2:AF049897 9196 L78814 Mycobacterium leprae cosmid B1554 DNA sequence.

L78813 Mycobacterium leprae cosmid 61551 DNA sequence.

AF049897 Corynebacterium glutamicum N-acetylglutamylphosphate reductase (argC), omnithine acetyltransfe rase (argJ), N-acetylglutamate kinase (argB), aoetylomithine transamInase (argD). omnithine carbamoyltransferase (argF), arginine repressor (argR), arginlnosucdinate synttiase (argG), and argininosuccinate lyase (argH) genes, complete cds.

AF005242 Corynebacterium glutamicuim N-aoetylglutamate-5-sermlaldehyde dehydrogenase (argC) gene, complete cds.

X86157 C.glutamicumn argC, argJ, argB, argD, and argF genes.

AF049897 Corynebactenium glutamicum N-acetylglutamylphosphate red uctase (argC).

omfthine acetyftransferase (argJ), N-acoetylgluta mate kinase (argB), acetylomithine transaminase (argD), omnithine carbamnoyltransferase (argF), arginine repressor (argR), argininosuccinate synthase (argG), and argininosuccinate lyase (argH) genes, complete cds.

AF005242 Corynebacterium glutamicum N-acetylglutamate-5-semialdehyde dehydrogenase (argC) gene, complete cds.

X861 57 C.glutamicum argC. argJ, argB., argD, and argF genes.

X86157 O~glutamicum argC, argJ, argB, argD, and argF genes.

AF049897 Corynebactenum glutamicum N-acetylglutamnylphosphate reductase (argC).

omnithine acetyftransferase (argJ), N-acetylglutamnate kinase (argB), acetylornithine transaminase (argD), omfthine carbamnoyltransferase (argF).

arglnine repressor (argR), argininosuccinate synthase (argG), and argininosuccinate lyase (argH) genes, complete cds.

178811 Mycobacterium leprae cosmld El1133 DNA sequence.

AF049897 Corynebacterium glutamicum N-acetylglutamylphosphate reductase (argC).

ornithine acetyltransferase (argJ), N-acetylglutamnate kinase (argB).

acetylornithine transaminase (argD), omithine carbamnoyltransferase (argF), arginine repressor (argR), argininosuccinate synthase (argG), and argininosuccinate lyase (argH-) genes, complete cds.

Arabidopsis thaliana Arabidopsis thallana Mycobacterium tuberculosis Mycobacterium leprae Mycobacterium leprae Corynebacterum glutamlcum Corynebactenium glutamicum Corynebacterium glutamicum Corynebacteriumn glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Mycobacterium leprae Corynebacterium glutamicum 34,123 3-Nov-98 31.260 7-Nov-98 34,281 26-Jun-98 62.904 17-Jun-98 36,648 15-Jun-96 36,648 15-Jun-96 99,104 1-Jul-98 99.224 2-Jul-97 100,000 25-Jul-96 98,551 1-Jul-98 98,477 2-Jul-9 100,000 25-Jul-96 99,767 25-Jul-96 99,378 1-Jul-98 55,504 15-Jun-96 100,000 1-Jul-98

W

rxa02157 1296 GB BA1:CGARGCJB

D

GBBA2:AE001816 GB-BA2:AF049897 GB.BA1 :CGARGCJB 0 GB-BA:MTCYO6HI I GB-BA2AF049897 rxa02I58 1080 GB..BA2:AF03I 518 GBBAI :CGARGCJB

D

GB-BA2:AF049897 Table 4 (continued) 4355 X86157 C.glutamicum argC. argJ, argB, argD, and argF genes.

10007 AE001816 Thermnotoga marftima section 128 of 136 of the complete genome.

9196 AF049897 Corynebacterium glutamicum N-acetylglutamnylphosphate reductase (argC), ornithine acetyltransferase (argJ), N-acetylglutamnate kinase (argB), acetylomithine transamlnase (argD), omithine carbamnoyltransferase (argF), arginine repressor (argR), argininosuccinate synthase (argG), and argininosuccinate lyase (argH) genes, complete cds.

4355 X86157 C.glutamlcum argC, argJ, argB, argD, and argF genes.

38000 Z85982 Mycobacterium tuberculosis H37Rv complete genome; segment 73/162.

9196 AF049897 Corynebactenium glutamicum N-acetylglutamnylphosphate reductase (argC), ornithine acetyltransferase (argJ), N-acetylglutamnate kinase (argB), acetylomnithine transaminase (argD), omithine carbamnoyltransferase (argF).

arginine repressor (argR), arglninosuccinate synthase (argG). and argininosuccinate lyase (argH) genes, complete cds.

2045 AF031518 Corynebacterlum glutamicum omnithine carbamnolytransferase (argF) gene, complete eds.

4355 X86157 C.glutamicum argC, argJ, argB, argO, and argF genes.

9196 AF049897 Corynebacterlum glutamicum N-acetylglutamylphosphate reductase (argC), omnithine acetyltransferase (argJ). N-acetylglutamate kinase (argB).

acetylornithine transaminase (argD), omnithine carbamnoyltransferase (argF), arginine repressor (argR), argininosuccinate synthase (argG). and argininosuccinate lyase (argH) genes, complete cds.

2045 AF031518 Corynebacterium glutamicum omnithine carbamolytransferase (argF) gene, complete cds.

516 AF041436 Corynebacterium glutamicum arginine repressor (argR) gene, complete cds.

9196 AF049897 Corynebactenium glutamicum N-acetylglutamnylphosphate reductase (argC), omnithine acetyltransferase (argJ), N-acetylglutamnate kinase (argB), acetylomithine transaminase (argD), omfthine carbamnoyltransferase (argF), arginine repressor (argR), argininosuccinate synthase (arjG). and argininosuccinate lyase (argH) genes, complete cds.

1206 AF030520 Corynebacterium glutamicum argininosuccinate synthetase (argG) gene, complete cds.

1909 Z491 11 S.clavuligerus argG gene and argH gene (partial).

9196 AF049897 Corynebacterium glutamicumn N-acetylglutamnylphosphate reductase (argC), ornithine acetyltransferase (argJ), N-acetylglutamnate kinase (argB), aoetylornithine transaminase (arqO), ornithine carbamnoyltransferase (argF).

arginine repressor (argR), argininosuccinate synthase (argO), and argininosuccinate lyase (argH) genes, complete cds.

Corynebacterium glutamicum Thermotoga maritima Corynebacterium glutamnicum Corynebacterium glutamicum Mycobacterium tuberculosis Corynebactenumn glutamicum Corynebadterum glutamicum Corynebacterium glutamicum Corynebacterium gkitamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterlum glutamicum Corynebacterium glutamicum Streptomyces clavuligerus Corynebactertum glutamicum 100,000 25-Jul-96 50,238 2-Jun-99 0 99,612 1-Jul-98 99,612 25-Jul-96 57,278 17-Jun-98 100,000 1-Jul-98 99,898 5-Jan-99 100,000 25-Ju-96 99,843 1-Jul-98 88,679 5-Jan-99 100,000 5-Janv-99 99.774 1-Jul-98 99,834 19-Nov-97 65,913 22-Apr-96 88,524 1-'Jul-98 rxaO2159 636 GBBA2:AFO31 518 GB..BA2:AF041436 GB,,,BA2.AF049897 rxa02I6O 1326 GB..6A2:AF030520 GB BA1:SCARGGH GB..9A2:AF049897 rxa02162 1554 GB.BA2:AF048764 1437 GB-BA1:MTCYO6H1 1 38000 rxa02176 1251 GB-BA1:MTCY31 37630 GB-BAI :CGGLTG 3013 GBPL2:PGU65399 2700 rxa02I89 861 GBLPR3:AC002468 115888 GB,.BA1:MSGB1 970C 39399

S

GB PR3AC002468 115888 rxa02193 1701 GB-BA1:BRLASPA 1987 GBPAT:E04307 1581 GBBA1:ECOUW493 338534 rxa02194 966 GB-BA2.AF050166 840 GB-BA1:BRLASPA 1987 GB-PAT:E08649 188 rxa02195 393 GB-BA2AF086704 264 GB-BA1:EAY17145 6019 GBSTS:G01 195 332 rxa02197 551 GB-BA1:MTCV26I 27322 GB,,BA1:MLCB2533 40245 GB.BA1:U00017 42157 rxa02198 2599 GB-BA1:U00017 42157 GBBA1 MLCB2533 40245 GB-BA1 :MTCY26I 27322 rxa02208 1025 GB-BAI:U00017 42157 AF048764 Z85982 Z73101 X66112 U65399 AC002468 L78815 AC002468 D25316 E04307 U 14003 AF0501 66 D25316 E08649 AF086704 Y17145 G01195 Z97559 AL035310 U00017 U00017 AL035310 Z97559 U00017 Table 4 (continued) Corynebacterium glutamicum argininosuccinate lyase (argl-) gene,complete cds.

Mycobacterium tuberculosis H37Rv complete genome; segment 73/162.

Mycobacterium tuberculosis H37Rv complete genome; segment 41/1 62.

C.glutamicum glt gene for citrate synthase and ORF.

Basidiomycete CECT 20197 phenoloxidase (poxi) gene, complete cds.

Human Chromosome 15q26.1 PAC clone pDJ4l7d7, complete sequence.

Myobacterium Ieprae cosmid B 1970 DNA sequence.

Human Chromosome 15q26.1 PAC clone pDJ4l7d7, complete sequence.

Brevibacterlum flavumn aspA gene for aspartase, complete cds.

DNA encoding Brevibacterium fiavumn aspartase.

Escheenchia coli K-12 chromosomal region from 92.8 to 00.1 minutes.

Corynebacterium glutamicum ATP phosphonibosyltransferase (hisG) gene, complete cds.

Brevibacterlum flavumn aspA gene for aspartase, complete cds.

DNA encoding part of aspartase from coryneform bacteria.

Corynebacterium glutamicum phosphoribosyl-ATP-pyrophosphohydrolase (hisE) gene, complete cds.

Eubacterium acidaminophilum grdR, grdl. grdH genes and partial ldc, grdT genes.

fruit fly STS Dm1 930 clone 0S06959 T7.

Mycobacterlum tuberculosis H37Rv complete genome; segment 95/162.

Mycobacterium leprae cosmid B2533.

Mycobacterium leprae cosmid B21 26.

Mycobactenium leprae cosmid B2126.

Mycobacterlum leprae cosmid B2533.

Mycobacterium tuberculosis H37Rv complete genome; segment 95/1 62.

Mycobacterium leprae cosmid B2126.

Corynebacterium glutamicum Mycobacterium tuberculosis Mycobacterium tuberculosis Corynebacterlum glutamicum basidiomycete CECT 20197 Homo sapiens Mycobacterium leprae Homo sapiens Corynebacterium glutamicum Corynebactedum glytamicum Escherichla col Corynebacterium glutamicum Corynebacteriumn glutamicum Corynebacterium glutamicum Corynebacterlum glutamicum Eubacterium acidaminophilum Drosophila melanogaster Mycobacterium tuberculosis Mycobacterum leprae Mycobacterlum leprae Mycobacterium leprae Mycobacterium leprae Mycobacterium tuberculosis Mycobacterium leprae 87,561 64,732 36,998 39,910 38,474 35,941 40,286 33,689 99.353 99,367 37,651 98,214 93,805 100.000 100,000 39,075 35.542 33.938 65,517 36,770 38,674 65,465 37,577 59,823 1-Jul-98 17-Jun-98 1 7-Jun-98 17-Feb-95 1 9-Jul-97 16-Sep-98 15-Jun-96 16-Sep-98 6-Feb-99 29-Sep-97 17-Apr-96 5-Jan-99 6-Feb-99 29-Sep-97 8-Feb-99 5-Aug-98 28-Feb-95 17-Jun-98 27-Aug-99 01-MAR- 1994 01-MAR- 1994 27-Aug-99 17-Jun-98 01-MAR- 1994 GB-BA1 :AP000063 185300 AP000063 Aeropyrum pemnix genomic DNA, section 6/7. Arprmpmx3,4 2Jn9 Aeropyrum pernix 39,442 22,Jur,99 rxa02229 948 rxa02234 3462 nca02235 727 GB-PR4:AC006236 GB..BAI :MSGY154 GBBAI:MTCYIS4 GBBA1 :U00019 GB BAI :MSGB937C

S

GB-BA1:MTCY2B1 2 G6._BA2-U01072 GB..A1 :MSU91572 40221 13935 36033 38914 20431 4393 960 GB-HTG3AC009364 192791 GByHTG3AC009364 192791 rxa02237 693 GB-BA1 :MTCY21 64 39150 GB-BA2:AF077324 5228 GB-EST22:AU017763 586 rxa02239 1389 GB-BAI :MTCY21 64 39150 GB..,HTGACO 10745 193862 GB...HTG3AC010745 193862 rxa02240 1344 EM-PAT:E09855 1239 127593 AC006236 AD000002 Z98209 U00019 178820 Z81 011 U01072 U9 1572 AC009364 AC009364 Z80108 AF077324 AU01 7763 Z801 08 AC01 0745 AC010745 E09855 A37831 AF1 17274 ABOO3693 Table 4 (continued) Homo sapiens chromosome 17. clone hCiT.162..E.12, complete sequence.

Mycobacterium tuberculosis sequence from clone yl 54.

Mycobacterium tuberculosis H37Rv complete genome; segment 121/1 62.

Mycobacterium leprae cosmid B2235.

Mycobacterium leprae cosmid B937 DNA sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 611162.

Mycobacterium bovis BCG orotidine-5-monophosphate decarboxylase (uraA) gene.

Mycobacterium smegmatis carbamoyl phosphate synthetase (pyrAB) gene, partial cds and orotidine 5-nionophosphate decarboxylase (pyrF) gene, complete cds.

Homo sapiens chromosome 7, -SEQUENCING IN PROGRESS ~,57 unordered pieces.

Homo sapiens chromosome 7, SEQUENCING IN PROGRESS ~,57 unordered pieces.

Mycobacterium tuberculosis H37Rv complete genome: segment 62/162.

Rhodococcus equi strain 103 plasmld RE-VP1 fragment f.

AUO1 7763 Mouse two-cell stage embryo cONA Mus musculus cONA clone J0744A04 mRNA sequence.

Mycobacterium tuberculosis H37Rv complete genome., segment 62/162.

Homo sapiens clone NH0549D18, -SEQUENCING IN PROGRESS ,30 unordered pieces.

Homo sapiens clone NH-1549D18, -SEQUENCING IN PROGRESS .30 unordered pieces.

gDNA encoding S-adenosylmethionine synthetase.

Homo sapiens Mycobacterium tuberculosis Mycobacterium tuberculosis Mycobacterium leprae Mycobacterium leprae Mycobacterium tuberculosis Mycobacterium bovis Mycobacterlum smegmatis Homo sapiens Homo sapiens Mycobactenium tuberculosis Rhodococcus equi Mus musculus Mycobacterium tuberculosis Home sapiens Homo sapiens Corynebac terium 53.541 40.407 40.541 66,027 71,723 67,101 60,870 37,191 29-DEC- 0 37,994 37,994 55.844 41,185 38,616 56,282 36,772 36,772 99,515 63,568 65.000 52,909 1998 03-DEC- 1996 17-Jun-98 01-MAR- 1994 15-Jun,-96 18-Jun-98 22-DEC- 1993 22-MAR- 1997 1 -Sep-99 1-Sep-99 23-Jun-98 5-Nov-98 19-OCT.

1998 23-Jun-98 21-Sep-99 21-Sep-99 07-OCT- 1997 (Re.

52, Created) 1997 31-MAR- 1999 03-OCT- 1997 (Re.

52, Cre~ted) 0 0 0 GByAT:A37831 5392 GB-BA2:AF1 17274 2303 EM-BAI AB003693 5589 glutamicum Sequence 1 from Patent W09408014. Streptomyces; pristinaespiralis Streptomyces spectabiiis flavoprotein homolog Dfp (d0p) gene, partial cds; and Streptomyces spectabillis S-adenosylmethionine synthetase (metK) gene, complete cds.

Corynebacterium ammoniagenes DNA for rib operon, complete cds. Corynebacterium ammoniagenes rxa02246 1107 GBYPAT:E07957 5589 GB-PAT:132742 5589 rxa02247 756 GBYAT:132743 2689 EM-BA1 AB003693 5589 GB _AT:I327412 5589 rxa02248 1389 GB PAT:132742 5589 EM-BAl :A8003693 5589 GB _PAT:E07957 5589 rxa02249 *600 GB-PAT:E07957 5589 GB PAT:132742 5589 G13 AT:132743 2689 rxa02250 643 GB7PAT:E07957 5589 GBPAT:132742 5589 EM..6A1:AB003693 5589 rxa02262 1269 GB-BA1 :CGL007732 4460 GB..BA1:CGAMTGEN 2028

E

GB VI:HEHCMVCG 2293~ rxa02263 488 GB-BA1 :CGL007732 4460 Table 4 (continued) E07957 gDNA encoding at least guanosine triphosphate cyciohydrolase and riboflavin Corynebacterium synthase. ammoniagenes 132742 Sequence 1 from patent US 5589355. Unknown.

132743 Sequence 2 from patent US 5589355. Unknown.

AB003693 Corynebactenium ammonlagenes DNA for rib operon. complete cds. Corynebacterium ammoniagenes 132742 132742 A8003693 Sequence 1 from patent US 5589355.

Sequence 1 from patent US 5589355.

Corynebacterium ammoniagenes DNA for rib operon, complete ods.

Unknown.

Unknown.

Corynebacterium ammoniagenes 52,909 29-Sep-97 52,909 6-Feb-97 57,937 6-Feb-97 57,937 03-OCT- 1997 (Rel.

52, Created) 57,937 6-Feb-97 61,843 6-Feb-97 61,843 03-OCT- 1997 (Rel.

52, Created) 61,843 29-Sep-97 64,346 29-Sep-97 E07957 gDNA encoding at least guanosine triphosphate cyciohydrolase and riboflavin Corynebacterium synthase. ammoniagenes E07957 gONA encoding at least guanosine triphosphate cyciohydrolase and riboflavin Corynebacterium synthase. ammoniagenes 132742 Sequence I from patent US 5589355. Unknown.

132743 Sequence 2 from patent US 5589355. Unknown.

E07957 gDNA encoding at least guanosine triphosphate cyciohydrolase and riboflavin Corynebacterium synthase. ammoniagenes 132742 Sequence I from patent US 5589355. Unknown.

ABO03693 Corynebacterium ammoniagenes DNA for rib operon, complete cds. Corynebacterium ammoniagenes 64,346 64,346 56.318 6-Feb-97 6-Feb-97 29-Sep-97 AJ007'732 Corynebacterlum glutamicum 3' ppc gene. secG gene, amt gene, ocd gene and 5' soxA gene.

X93513 C.glutamicum amt gene.

A4 X17403 AJO07732 Human cytomnegalovirus strain AD169 complete genome.

Corynebacterium giutamicum 3'ppc gene, secG gene, amt gene. ood gene and 5' soxA gene.

Corynebacterium giutamicum T ppc gene, secG gene, amt gene, ood gene and 5' soxA gene.

Corynebacteriumgiutamicum Corynebacterium glutamicum human herpesvirus 5 Corynebacterium glutamicum Corynebactenium glutamicum 56,318 6-Feb-97 56,318 03-OCT- 1997 (Rel.

52, Created) 100,000 7-Jan-99 100,000 29-MAY- 1996 38,651 10-Feb-99 100,000 7-Jan-99 37.526 7-Jan-99 96,928 08-OCT- 1997 (Rel.

52, Created) 96.781 7-Aug-98 36.264 20-Feb-99 36.,197 .17-MAR- 1999:-..

GB-BAI:CGL007732 4460 AJ007732 rxa02272 1368 EMPAT:E09373 GBBA1 :D38505 GB TG2:AC006595 rxa02281 1545 GB7GssI 2:A041 101 0 1591 E09373 Creatinine deiminase gene. Bacillus sp.

Bacillus sp.

Homo sapiens Homo sapiens 1591 146070 551 D38505 AC006595 A041 1010 Bacillus sp. gene for creatinine deaminase, complete ods.

Homo sapiens. SEQUENCING IN PROGRESS 4 unordered pieces.

HS_2257 Bi HO2-MR CIT Approved Human Genomic Sperm Library D Homo sapiens genomic clone Plate=2257 CoI=3 Row-P, genomic survey sequence.

GB-EST23AI128623 363 GB-PL2:ATACO07O1 9 102335 GBBA2:AF116184 540 GB-GSS9AQ164310 507 Al 128623 AC00701 9 AF1 16184 AQl 64310 rxa02299 531 rxaO23ll 813 rxa02315 1752 rxa02318 402 rxa0231 9 1080 GB Vl:MH68TKH 4557 X93468 GCHTG4AC006091 176878 AC006091 GByHTG4AC006091 176878 AC006091 GB-BA2:RRU65510 16259 U6551 0 Table 4 (continued) qa62c0l .s1 Soares..fetal-heart..NbHH19W Holmo sapiens cIDNA clone Homo sapiens IMAGE:1691328 3T, mRNA sequence.

Arabidopsis thaliana chromosome 11 BAC F7D8 genornic sequence, complete Arabidopsis thaliana sequence.

Corynebacterium glutamicum L-aspartate-alpha-decarboxylase precursor Corynebacteriumn (pan 0) gene, complete eds. glutamicum HS_2171,A2_EO1YMR CIT Approved Human Geriomlc Sperm Library D Homo sapiens Home sapiens genomic clone Plate=2171 CoI=2 Row-I, genomic survey sequence.

Murine herpesvirus type 68 thymidine kinase and glycoprotein H genes. murine herpesvirus 68 Drosophila melanogaster chromosome 3 clone BACR48GO5 (0475) RPCI-98 Drosophila melanogaster 48.G.5 map 91F1-91F13 strain y; cni bw sp, -SEQUENCING IN PROGRESS 4 unordered pieces.

Drosophila melanogaster chromosome 3 clone BACR48GO5 (13475) RPCI-98 Drosophila melanogaster 48.G.5 map 91FI-91F13 strain y; cn bw sp, -SEQUENCING IN PROGRESS 4 unordered pieces.

Rhodospirillum rubrum CO-induced hydrogenase operon (cooM. cooK, cool-, Rhodospinilium rubrum cooX, cooU, cooH) genes, Iron sulfur protein (cooF) gene, carbon monoxide dehydrogenase (cooS) gene, carbon monoxide dehydrogenase accessory proteins (cooC, cooT, cooJ) genes, putative transcriptional activator (cooA) gene, nlcotinate-nucleotlde pyrophosphorylase (nadC) gene, complete cds, L-aspartate oxidase (nadB) gene, and alkyl hydroperoxide red uctase (ahpC) gene, partial cds.

Mycobacterlum tuberculosis sequence from clone y224. Mycobacteriumn tuberculosis Mycobacterium tuberculosis H37Rv complete genome; segment 28/162. Mycobacterium tuberculosis Mycobacterlumn tuberculosis sequence from clone y224. Mycobacteriumn tuberculosis Homo sapiens chromosome 5 clone CIT-HSPC 303E13, SEQUENCING Homo sapiens IN PROGRESS 3 ordered pieces.

Home sapiens chromosome 5 clone CIT-HSPC 303E1 3, SEQUENCING Homo sapiens IN PROGRESS 3 ordered pieces.

Homo sapiens chromosome 5 clone CIT978SKB-81 K21,~ SEQUENCING Home sapiens IN PROGRESS 3 ordered pieces.

Mycobacterlum tuberculosis sequence from clone y224. Mycobacterium tuberculosis Mycobacterium tuberculosis H37Rv complete genome: segment 28/162. Mycobacterium tuberculosis ub83h02.rl Soares 2NbMT Mus musculus cDNA clone IMAGE:1395123 Mus musculus sequence.

37,017 33,988 100,000 37,278 40,288 36,454 36,454 37,828 49.418 49,360 38,150 35,821 35,821 36,181 37,792 37,792 35,084 1998 16-MAR- 1999 02-MAY- 1999 16-OCT.

1998 3-Sep-96 27-OCT.

1999 27-OCT- 1999 9-Apr-97 03-DEC- 1996 1 7-Jun-98 03-DEC- 1996 06-OCT- 1999 06-OCT- 1999 06-OCT- 1999 03-DEC- 1996 17-Jun-98 2-Sep-.98 GBBA1:M5GY224 GB,_BA1 :MTY25D1 0 GB-BA1:MSGY224 GB-HTG3:ACOI 1348 GB-HTG3:AC01 1348 GB-HTG3:AC011412 GBBaA1 :MSGY224 GB-BA1 :MTY25D1O GB-EST23:AI1 17213 40051 40838 40051 111083 111083 89234 40051 40838 476 AD000004 Z95558 AD000004 AC0l 1348 AC01 1348 ACOI 1412 AD000004 Z95558 Al 17213 rxa02345 1320 rxa02350 618 rxa02373 1038 rxa02375 1350 rxa02380 777 rxa02382 1419 nca02400 693 rxa02432 1098

GB-BAI:BAPURKE

GB..BA1 :MTCY71 GB-BA1:MTCY71 GB-BAI :BAPURKE GB-PLI :SCI 3OKBXV Gd'PL1:SCXVORFS GB .PAT:E003111 GCPAT:106030 GByPAT:100836 GBBA2:CGU31 230 GBJ-ITG3:AC009946 GB!TG3:AC009946 GB8BA1:MTCY253 GBJ{TG4:ACO1 0658 GByTG4AC010658 GB-BAI :CGPROAGE

N

GB.,BA1 :MTCY428 GB BA2:CGU31230 GB-BAI :CGACEA GByAT:186191 GB PAT:I113693 GB-GSSIS:A060684 2 2582 42729 42729 2582 129528 50984 1853 1853 1853 3005 1169072 169072 41230 1 20754 120754 1783 26914 3005 2427 2135 2135 574 X91189 Z92771 Z92771 X91 189 X94335 X90518 E00311 106030 100836 U31230 AC009946 AC009946 Z81 368 AC010658 AC010658 X82929 Z81451 U31230 X75504 186191 113693 AQ606842 Table 4 (continued) Bammonlagenes purK and purE genes.

Mycobactenium tuberculosis H37Rv complete genome, segment 141/162.

Mycobacterium tuberculosis H37Rv complete genome: segment 141/162.

Bammoniagenes purK and purE genes.

S.cerevlslae 130kb DNA fragment from chromosome XV.

S.cerevlsiae DNA of 51 Kb from chromosome XV right arm.

DNA coding of 2,5-diketogluconic acid reductase.

Sequence 4 from Patent EP 0305608.

Sequence 1 from Patent US 4758514.

Corynebacterium glutamicum Obg protein homolog gene, partial cds, gamma glutamyl kinase (proB) gene, complete cds, and (unkdh) gene. complete cds.

Homo sapiens clone NHOO12C17, SEQUENCING IN PROGRESS I, unordered pieces.

Homo sapiens clone NH0012C17, SEQUENCING IN PROGRESS I, unordered pieces.

Mycobacterium tuberculosis H37Rv complete genome; segment 106/162.

Drosophila melanogaster chromosome 3LI75C1 clone RPCI98-3B320, SEQUENCING IN PROGRESS 78 unordered pieces.

Drosophila melanogaster chromosome 3L175C1 clone RPCI98-3B20.

SEQUENCING IN PROGRESS 78 unordered pieces.

C.glutamicum proA gene.

Mycobacterium tuberculosis H37Rv complete genome; segment 107/162.

Corynebacterium glutamicum Obg protein homolog gene, partial cds, gamma glutamyl kinase (proB) gene, complete cds, and (unkdh) gene, complete cds.

C.glutamicum aceA gene and thIX genes (partial).

Sequence 3 from patent US 5700661.

Sequence 3 from patent US 5439822.

HS,.5404..B2E07)7A RPCI-11I Human Male BAC Uibrary Homo sapiens genomic clone Plate=980 Col=14 Row--J, genomlc survey sequence.

Corynebacterium ammoniagenes Mycobactenium tuberculosis Mycobacterium tuberculosis Corynebacterium ammoniagenes Saccharomyces cerevislae Saccharomyces cerevislae unidentified Unknown.

Unknown.

Corynebactenium glutamicum Homo sapiens Homo sapiens Mycobacterium tuberculosis Drosophila melanogaster Drosophila melanogaster Corynebacteium glutamicum Mycobacterium tuberculosis Corynebactenium glutamicum Corynebacterlum glutamicum Unknown.

Unknown.

Homo sapiens 61,731 39,624 39.847 64,286 36,617 36,617 56,123 56,220 56,220 99,332 36.115 36,115 38.088 35,817 35,817 98,802 38.054 98,529 100,000 100,000 100,000 39,716 14-Jan-97 10-Feb-99 10-Feb-99 14-Jan-97 115-Jul-97 1 29-Sep-97 02-DEC- 1994 21-MAY- 1993 2-Aug-96 8-Sep-99 8-Sep-99 17-Jun-98 16-OCT- 1999 116-OCT- 1999 23-Jan-97 17-Jun-98 2-Aug-96 9-Sep-94 10-Jun-98 26-Sep-e05 10-jur9 rxa02458 1413 GB.EST1 :TD5804 406 GByPL1:AB006699 77363 GB.BA2:AF1 14233 1852 GB-EST37:AW01306 578 GBGSS1 5:A65002 728 7 GB-BA1 :MTCY359 36021 Table 4 (continued) T05804 EST03693 Fetal brain, Stratagene (cat#936206) Homo sapiens eDNA clone Homo sapiens HFBDG63 similar to EST containing Alu repeat, mRNA sequence.

AB006699 Arabidopsis thallana genomic DNA, chromosome 5, P1 clone: MDJ22. Arabidopsis th~ complete sequence.

AFi 14233 Corynebacterium glutamicum 5-enolpyruvyishikimate 3-phosphate synthase Corynebactedu (aroA) gene, complete cds. glutamicum AW013061 ODT-0033 Winter flounder ovary Pleuronectes americanus cDNA clone ODT- Pleuronectes a 0033 5' similar to FRUCTOSE-BISPHOSPHATE ALDOLASE B (LIVER), mRNA sequence.

A0650027 Sheared DNA-5L2.TF Sheared DNA Trypanosoma brucei genomic clone Trypanosoma Sheared DNA-5L2, genomic survey sequence.

Z83859 Mycobacterium tuberculosis H37Rv complete genome; segment 84/162. Mycobacteriun aliana imericanus brucei 37,915 30-Jun-93 35,526 20-Nov-99 100,000 7-Feb-99 39,175 10-Sep-99 rxa02469 1554 rxa02497 1050 GB BAI:MLCBI788 GB BA1:SCAJ1 0601 GBBA2:CGU311224 39228 4692 422 AL008609 AJO1 0601 U31 224 Mycobacterium leprae cosmid 61788.

Streptomyces coellcolor A3(2) DNA for whiD and whiK loci.

Corynebacterium glutamicum (ppx) gene, partial cds.

tuberculosis Mycobacterium leprae Streptomyces coelicolor Corynebacterlum glutamicum Mycobacterium tuberculosis Streptomyces coelicolor 39,281 22-Jun-99 39,634 17-Jun-98 59,343 27-Aug-99 48,899 17-Sep-98 96,445 2-Aug-96 59,429 117-Jun-98 39,510 1999 97,749 2-Aug-96 GBLBA1 :MTCY2OG9 37218 GBBAI:SCE7 16911 GB...6A2:CGU3I 225 1817 Z771 62 Mycobacterlum tuberculosis H37Rv complete genome; segment 25/162.

AL049819 Streptomyces coelicolor cosmid E7.

rxa02499 933 GB-HTG2:AC007984 129715 GB BA1 :MTCY2OG9 37218 U31 225 Corynebactenium glutamicum L-proline:NADP+ 5-oxidoreductase (proC) gene, Corynebacterium complete cds. glutamicum X13965 Neissenia gonorrhoeae pilA gene. Neisseria gonorrtroeae AC007984 Drosophila melanogaster chromosome 3 clone BACR05C1O (D781) RPCI-98 Drosophila melanogaster 05.C.10 map 97D-97E strain y; cii bw sp, SEQUENCING IN PROGRESS -,87 unordered pieces.

Z77162 Mycobacterium tuberculosis H37Rv complete genome; segment 25/1 62. Mycobacterium 43,249 33,406 30-Sep-93 2-Aug-99 rxaO2501 1188 rxa02503 522 rxa02504 681 rxa02516 1386 G6.6A1:U00018 GBYl:HE1CG GBkyR3:AC005328 GkP'R3:AC005545 GkP'R3:AC005328 GB,6A1 :MTCY2OG9 GkPR3:AC005328 GB! R3:AC005545 GB.BAI :MLCL536 GBBA1:U00013 152261 35414 43514 35414 37218 35414 43514 36224 X14112 AC005328 AC005545 AC005328 Z77162 AC005328 AC005545 Z99125 Herpes simplex virus (HSV) type 1 complete genome.

Homo sapiens chromosome 19, cosmid R26660, complete sequence.

Homo sapiens chromosome 19, cosmid R26634, completi sequence.

Homo sapiens chromosome 19, cosmid R26660, complete sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 25/162.

Homo sapiens chromosome 19, cosmld R26660, complete sequence.

Homo sapiens chromosome 19. cosmld R26634, complete sequence.

Mycobacterium leprae cosmid L536.

42991 U00018 Mycobactedrn leprae cosmid B2168.

tuberculosis Mycobacterium leprae human herpesvirus 1 Homo sapiens Homo sapiens Homo sapiens Mycobacterium tuberculosis Homo sapiens Homo sapiens Mycobacterium leprae Mycobacterium leprae 39,357 17-Jun-98 51,768 01-MAR- 1994 39,378 17-Apr-97 39,922 28-Jul-98 39,922 3-Sep-98 34,911 28-Jul-98 54,940 17-Jun-98 41,265 28-Jul-98 41,265 3-Sep-98 37,723 04-DEC- 1998 37,723 01-MAR- 1994 35881 U00013 Mycobacterium leprae cosmid B1496.

Table 4 (continued) 32808 AL021 184 Mycobacterium tuberculosis H37Rv complete genome: segment 64/162.

GB..BAI :MTV007 Mycobacterium tuberculosis 61.335 17-Jun-98 rxa02517 570 rxa02532 1170 rxa02536 879 rxa02550 1434 rxa02559 1026 rxa02622 1683 GBBAI:MLCL536 36224 Z99125 GB.BA1:U00013 35881 U00013 GB BA1:SCC22 22115 AL096839 GB-OV:AF137219 831 AF137219 GB-EST3O:A1645057 301 A1645057 GB-EST20OAA822595 429 AA822595 GB-HTG2:AFI 30866 118874 AFi 30866 GBJ-ITG2:AF130866 118874 AF130866 GB...PL1ATT12J5 84499 AL035522 GB-BA1:MTCY279 9150 Z97991 GBA1:MSGB197OC 39399 L78815

S

GB-BA2:SC2H4 25970 AL031514 GB-BAI :MTVOO4 69350 AL009 198 GB PAT:128684 5100 128684 GB.8A1:MTU27357 5100 U27357 GB BA2:AEOOI 780 11997 *AE001780 GB.0V:AF084564 49254 AF064564 Mycobacterium leprae cosmid L536. Mycobactenumr leprae Mycobacterium leprae cosmid B1496. Mycobacterium leprae Streptomyces; coelicolor cosmid C22. Streptomyces coelicolor Amia calva mixed lineage leukemia-like protein (MII) gene, partial cds. Amia calva vs52alO.yl Stratagene mouse Tcell 937311 Mus musculus cDNA clone Mus musculus IMAGE: 1149882 mRNA sequence.

vsS2alO.rl Stratagene mouse Tcell 937311 Mus musculus cONA clone Mus musculus IMAGE: 1149882 mRNA sequence.

Homo sapiens chromosome 8 clone PAC 172N13 map 8q24, Homo sapiens SEQUENCING IN PROGRESS in unordered pieces.

Homo sapiens chromosome 8 clone PAC 172N13 map 8q24. Homo sapiens SEQUENCING IN PROGRESS unordered pieces.

Arabidopsis thaliana DNA chromosome 4, BAC clone T12.15 (ESSAII project). Arabidopsis thallana Mycobacterium tuberculosis H37Rv complete genome; segment 17/162. Mycobacteriumn tuberculosis Mycobacterium leprae cosn'id 61970 DNA sequence. Mycobacterium leprae Streptomyces coelicolor cosmld 2H4. Streptomyces coelicolor A3(2) Mycobacterlumn tuberculosis H37Rv complete genome; segment 144/162. Mycobacterium tuberculosis Sequence 1 from patent US 5573915. Unknown.

Mycobactenumr tuberculosis cydlopropane mycolic acid synthase (cmalI) Mycobacteriumn gene, complete cds. tuberculosis Thermotoga maritima section 92 of 136 of the complete genome. Thermotoga maritima Fugu rubripes neurofibromatosis type 1 (NFl), A-kinase anchor protein Fugu rubripes (AKAP84), BAW protein (BAW), and WSB1 protein (WSBI) genes, complete eds.

Fugu rubripes neurofibromatosis, type I (NFl), A-kinase anchor protein Fugu rubrlpes (AKAP84), BAW protein (BAW). and WSB1I protein (WSB1) genes, complete cds.

HS_5268 Al G09..SP6E RPCI-1 1 Human Male BAC Library Homo sapiens Homo sapiens genomic clone Plate=844 CoI=17 Row--M. genomic survey sequence.

Homo sapiens chromosome 9 clone RPII1-111 M7 map 9, WORKING DRAFT Homo sapiens SEQUENCE, 51 unordered pieces.

HS55014_A2_C1 2_T7A RPCI-1I Human Male BAC Library Homo sapiens Homo sapiens genomic clone Plate=590 Col=24 Row-E, genamic survey sequence.

37.018 37,018 37,071 36,853 41,860 42,353 40,754 40,754 35,063 37,773 39,024 37,906 47.358 39,138 39,138 44,914 39,732 04-DEC- 1998 01-MAR- 1994 1 2-Jul-99 7-Sep-99 29-Apr-99 1 7-Feb-98 21 -MAR- 1999 21 -MAR- 1999 24-Feb-99 17-Jun-98 1 5-Jun-96 19-OCT- 11999 18-Jun-98 6-Feb-97- 26-Sep-95 2-Jun-99 17-Aug-99 GB..9V:AF064564 49254 AF064564 36,703 17-Aug-99 38.801 26-Aug-99 rxa02623 714 GB_G555AQ818728 444 AQ818728 GBHTG5:ACOI10O83 GB-GSS6:AQ826948 198586 544 ACOI 1083 AQ826948 35,714 39,146 1 9-Nov-99 27-Aug-'99 rxa02629 708 rxa02645 1953 rxaO2646 1392 rxa02648 1326 GByI:BRSMGP

GBVI:BRSMGP

GB..PAT:A45577 1925 GBPAT:A455811 1925 GB.BA1 :CORILVA 1925 GB-BA1 :CORILVA 1925 GByAT:A45585 1925 GB.YAT:A45583 1925 GBOV:ICTCNC 2049 GB-EST11 1:AA265464 345 GB-GSS8:AQ006950 480 Table 4 (continued) M86652 Bovine respiratory syncytial virus membrane glycoprotein mRNA, complete cds.

M86652 Bovine respiratory syncytial virus membrane gtycoprotein mRNA, complete cds.

A45577 Sequence 1 from Patent W09519442.

A45581 Sequence 5 from Patent WO9519442.

L01 508 Corynebacterium glutamicum threonine dehydratase (ilvA) gene, complete cds.

L01508 .Corynebacterlum glutamfcum threonine dehydratase (ilvA) gene, complete cds.

A45585 Sequence 9 from Patent W09519442.

A45583 Sequence 7 from Patent W095 19442.

M831 I1I Ictalurus punctatus cyclic nucleotide-gated channel RNA sequence.

AA265464 mx9lcO6.rl Soares mouse NML Mus musculus cDNA clone IMAGE:693706 mRNA sequence.

AQ006950 CIT-HSP-2294E14.TR CIT-HSP Homo sapiens genomic clone 2294E14.

genomic survey sequence.

Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Corynebacterium giutamicum Ictalurus punctatus Mus musculus Homo sapiens 39,130 39,130 39,130 99.138 99,066 99,066 38,402 38,655 36,074 Bovine respiratory syncytial 37,013 virus Bovine respiratory syncytial 37,013 28-Apr-93 28-Apr-93 07-MAR- 1997 07-MAR.

1997 26-Apr-93 26-Apr-93 07-MAR- 1997 07-MAR- 1997 24-MAY- 1993 1997 27-Jun-98 rxa02653 rxa02687 1068 rxa02717 1005 rxa02754 1461 GB-BA1 :CORPHEA GB.YAT:E04483 GB _PAT:061 10 GBJ'L1 :HVCH-4H GB_PR2:HS31OH5 GB!PR3AC004754 1088 948 948 59748 29718 M1 3774 E04483 E061 10 Y14573 Z69705 C.glutamicum pheA gene encoding prephenate dehydratase, complete cds.

DNA encoding prephenate dehydratase.

DNA encoding prephenate dehydratase.

Hordeum vulgare DNA for chromosome 4H.

Human DNA sequence from cosmid 31 OHS from a contig from the tip of the short arm of chromosome 16, spanning 2Mb of 16pl 3.3. Contains EST and CpG Island.

Homo sapiens chromosome 16, cosmid clone RT286 (LANL), complete sequence.

Drosophila melanogaster chromosom'e 3 clone BACR16118 (10815) RPCI-98 16.1.18 map 95A-95A strain y; en bw sp, -SEQUENCING IN PROGRESS-, 101 unordered pieces.

Corynebacterum glutamicum Corynebacterium glutamicum Corynebacterium glutamicum Hordeum vulgare Homo sapiens Homo sapiens Drosophila melanogaster 99,715 98,523 98,523 36,593 36,089 26-Apr-93 29-Sep-97 29-Sep-97 1999 22-Nov-99 39188 AC004754 36,089 28-MY- 1998 GB-HTG2:AC008223 130212 AC008223 32,757 2-Aug-99 nca02758 1422 rxa02771 678 rxa02772 1158 GByHTG2:AC008223 G6..BA1 :MTCY71 GB-HTGS:AC01 1678 GBHTG:AC01 1678 GB BA2AF064070 GB...8AAF038651 GBJN1 CELT 9834 GB.EST36:AV1 93572 GB..BA2.AF038651 Table 4 (continued) 130212 AC008223 Drosophila melanogaster chromosome 3 clone BACRI6118 (0815) RPCI-98 Drosophila mel 16.1.18 map 95A-95A stany; cnbwsp, SEQUENCING IN PROGRESS 101 unordered pieces.

42729 Z92771 Mycobacterlum tuberculosis H37Rv complete genome: segment 141/162. Mycobacterium tuberculosis 171967 AC011678 Homo sapiens clone 146B7, SEQUENCING IN PROGRESS 20 Homo sapiens unordered pieces.

171967 AC01 1678 Homo sapiens clone 14B7,~ SEQUENCING IN PROGRESS ~,20 Homo sapiens unordered pieces.

23183 AF064070 Burkhoideria pseudomailel putative dihydroorotase (pyiC) gene, partial cds: Burkholderia ps putative 1 -acyl-sn-glycerol-3-phosphate acyltransferase (pIsC), putative diadenosine tetraphosphatase (apaH). complete cds; type 11 0-antigen biosynthesis gene cluster, complete sequence; putative undecaprenyl phosphate N-acetyiglucosaminyltransferase, and putative UDP-glucose 4epimerase genes, complete cds; and putative galactosyl transferase gene, partial cds.

4077 AF038651 Corynebacterium giutamicum dipeptide-binding protein (dc!AE) gene, partial Corynebacterlu cds; adenine phosphoribosyltransferase (apt) and GTP pyrophosphokinase glutamicum (rel) genes, complete ods;, and unknown gene.

37121 U80438 Caenorhabditis elegans cosmid T19134. Caenorhabditis 360 AV193572 AV193572 Yuji Kohara unpublished cDNK-Strain N2 hermaphrodite embryo Caenorhabditis Caenorhabditis elegans cDNA clone yk618h8 mRNA sequence.

4077 AF038651 Corynebacterlum glutamicum dipeptide-binding protein (dciAE) gene, partial Corynebacteru cds; adenine phosphoribosyltransferase (apt) and GTP pyrophosphokinase glutamicum (rel) genes, complete cds; and unknown gene.

35946 Z77724 Mycobacterlum tuberculosis H37Rv complete genome; segment 114/162. Mycobacterium, tuberculosis 40429 U0001 1 Mycobacterium leprae cosmid BI1177. Mycobacterium 33818 Z83863 Mycobacterium tuberculosis H37Rv complete genome; segment 111/162. Mycobacteriumr tuberculosis 172931 AC006581 Homo sapiens i 2 p 2 i BAC RPC11-259018 (Roswell Park Cancer Institute Homo sapiens Human BAC Library) complete sequence.

172931 AC006581 Homo sapiens 12p 2 l BAC RPCI 1-259018 (Roswell Park Cancer Institute Homo sapiens Human BAC Library) complete sequence.

33818 Z83863 Mycobacterium tuberculosis H37Rv complete genome; segment 111/162. Mycobacteriumr tuberculosis 3694 M35 195 Chicken tyrosine kinase (cek2) mRNA, complete cds. Gallus gallus 5037 Z1 7372 M.smegmatis asd, ask-alpha, and ask-beta genes. Mycobacteriumr 169 A1223401 qg48gOl.xl Soares-testis-NHT Homo sapiens cDNA clone IMAGE:1838448 Homo sapiens 3 similar to WP:C25D7.8 CE08394 mRNA sequence.

eudomallei 37,838 35,331 33,807 36,929 anogaster 32,757 2-Aug-99 10-Feb-99 5-Nov-99 5-Nov-99 20-Jan-99 GBBA1 MTCY227 GBBA1 :UOOO1 1 rxa02790 1266 GB-BA1:MTCY159 GB-PR4:AC006581 GB-PR4:AC006581 rnxa02791 951 GB-BA1:MTCY159 GB..9V:CHKCEK2 GB...BA1:MSASOASK rxa02802 1194 GB-EST24:A1223401 m elegans elegans m l eprae smegmatis 43,836 48.588 99,914 38,339 38,996 37,640 37,906 35,280 39,765 38,937 38,495 40,828 04-DEC- 1996 22-Jul-99 14-Sep-98 1 7-Jun-98 01-MAR- 1994 1 7-Jun-98 3-Jun-99 3-Jun-99 17-Jun-98 28-Apr-93 9-Aug-94 27-0CT: 1998 99,852 14-Sep-98 GB-EST24AI223401 169 A1223401 rxa02814 494 nxa02843 608 rxs03205 963 rxs03223 1237 GBBA1 :MTCY7DI 11 GB-BA1 :MTCY7D1 1 GB PR1 :ISAJ2962 GB-BAI :CGAJ4934 G6BA1 :MTC1364 GB.BAI :MLU151 80 GB BA1:BLSIGBGN GB-EST21 :AA980237 GB..EST23:AE1158316 GB-JN 1:LMFL2743 GB..PR3:HSDJ61 B2 22070 22070 778 1160 29540 38675 2906 377 371 3838 119666 Z95120 Z95120 AJ002962 AJ004934 Z93777 U15180 Z49824 AA980237 All158316 AL031910 AL096710 Table 4 (continued) qg48gOl xl Soares~testis-NHT Homo sapiens cDNA clone IMAGE: 1838448 Homo sapiens 3 similar to WP:C25D7.8 CE08394 mRNA sequence.

Mycobacterium tuberculosis H37Rv complete genome; segment 138/162. Mycobacterium tuberculosis Mycobacterium tuberculosis H37Rv complete genome; segment 138/162. Myoobacterlum tuberculosis Homo sapiens mRNA for hB-FABP. Homo sapiens Corynebactenium glutamicum dapD gene, complete CDS. Corynebacterium glutamicum Mycobacterium tuberculosis H37Rv complete genome; segment 521162. Mycobacterium tuberculosis Mycobacterium leprae cosmid B1 756. Mycobacterium lel B.lactofermentum orf1 gene and sigB gene. Corynebacterium ua32a 12.r0 Soares...mammarygl1and.NbMMIG Mus musculus cONA clone Mus musculus IMAGE:348414 5 similar to TR:061025 061 025 HYPOTHETICAL 15.2 KD PROTEIN. mRNA sequence.

ud27c05.rl Soares thymus...2NbMT Mus musculus cDNA clone Mus mrusculus IMAGE147112 MRNA sequence.

Leishmania major Friedlin chromosome 4 oosnmid L2743. Leishmania major Human DNA sequence from clone RPI-61 B2 on chromosome 6p1 1.2-1 2.3 Homo sapiens Contains isoforrns 1 and 3 of BPAGI (bullous pemphigoid antigen I (23012400D), an exon of a gene similar to murine MACIF cytoskeletal protein.

STSs and GSSs, complete sequence.

Human DNA sequence from clone RPI-61 B2 on chromosome 6pll1.2-1 2 .3 Homo sapiens Contains isoforrns 1 and 3 of BPAG1 (bullous pemphigoid antigen I (230I240kD), an exon of a gene similar to murine MACIF cytoskeletal protein, STSs and GSSs, complete sequence.

58,418 40,496 39,826 100,000 37,710 prae 39.626 glutamicur@8,854 41,489 38,005 39,869 34.930 17-Jun-98 1 7-Jun-98 8-Jan-98 1 7-Jun-98 17-Jun-98 09-MAR- 1995 25-Apr-96 27-MAY- 1998 30-Sep-98 1999 1 7-DEC- 1999 40,828 27-OCT- GB-PR3:HSDJ6lB2 119666 AL096710 34,634 17-DEC- 1999 WO 01/00843 PCT/IB00/00923 -132- Exemplification Example 1: Preparation of total genomic DNA of Corynebacterium glutamicum ATCC 13032 A culture of Corynebacterium glutamicum (ATCC 13032) was grown overnight at 30 0 C with vigorous shaking in BHI medium (Difco). The cells were harvested by centrifugation, the supernatant was discarded and the cells were resuspended in 5 ml buffer-I of the original volume of the culture all indicated volumes have been calculated for 100 ml of culture volume). Composition of buffer-I: 140.34 g/l sucrose, .2.46 g/l MgSO, x 7H 2 0, 10 ml/l KH,PO, solution (100 g/l, adjusted to pH 6.7 with KOH), 50 ml/I M12 concentrate (10 g/l 1 g/l NaC1, 2 g/1 MgSO, x 7H,O, 0.2 g/l CaCIl, 0.5 g/l yeast extract (Difco), 10 ml/1 trace-elements-mix (200 mg/l FeSO, x H 2 0, 10 mg/1 ZnSO, x 7 H0, 3 mg/l MnCI, x 4 HO, 30 mg/l HBO, 20 mg/l CoCI, x 6 H0O, 1 mg/l NiCI, x 6 H,O, 3 mg/1 NaMoO, x 2 H 2 0, 500 mg/l complexing agent (EDTA or critic acid), 100 ml/l vitamins-mix (0.2 mg/l biotin, 0.2 mg/l folic acid, mg/l p-amino benzoic acid, 20 mg/l riboflavin, 40 mg/l ca-panthothenate, 140 mg/l nicotinic acid, 40 mg/l pyridoxole hydrochloride, 200 mg/l myo-inositol). Lysozyme was added to the suspension to a final concentration of 2.5 mg/ml. After an approximately 4 h incubation at 37'C, the cell wall was degraded and the resulting protoplasts are harvested by centrifugation. The pellet was washed once with 5 ml buffer-I and once with 5 ml TE-buffer (10 mM Tris-HC1, 1 mM EDTA, pH The pellet was resuspended in 4 ml TE-buffer and 0.5 ml SDS solution and 0.5 ml NaCI solution (5 M) are added. After adding ofproteinase K to a final concentration of 200 pg/ml, the suspension is incubated for ca.18 h at 37 0 C. The DNA was purified by extraction with phenol, phenol-chloroform-isoamylalcohol and chloroformisoamylalcohol using standard procedures. Then, the DNA was precipitated by adding 1/50 volume of 3 M sodium acetate and 2 volumes of ethanol, followed by a 30 min incubation at -20 0 C and a 30 min centrifugation at 12,000 rpm in a high speed centrifuge using a SS34 rotor (Sorvall). The DNA was dissolved in 1 ml TE-buffer containing pg/ml RNaseA and dialysed at 4 0 C against 1000 ml TE-buffer for at least 3 hours.

During this time, the buffer was exchanged 3 times. To aliquots of 0.4 ml of the dialysed DNA solution, 0.4 ml of 2 M LiCI and 0.8 ml of ethanol are added. After a 133 min incubation at -20 0 C, the DNA was collected by centrifugation (13,000 rpm, Biofuge Fresco, Heraeus, Hanau, Germany). The DNA pellet was dissolved in TE-buffer. DNA prepared by this procedure could be used for all purposes, including southern blotting or construction of genomic libraries.

Example 2: Construction of genomic libraries in Escherichia coli of Corynebacterium glutamicum ATCC13032 Using DNA prepared as described in Example 1, cosmid and plasmid libraries were constructed according to known and well established methods (see Sambrook, J. et al. (1989) "Molecular Cloning A Laboratory Manual", Cold Spring Harbor Laboratory Press, or Ausubel, F.M. et al. (1994) "Current Protocols in Molecular Biology", John Wiley Sons).

Any plasmid or cosmid could be used. Of particular use were the plasmids pBR322 (Sutcliffe, J.G. (1979) Proc. Natl. Acad. Sci. USA, 75:3737-3741); pACYC177 (Change Cohen (1978) J. Bacteriol 134:1141-1156), plasmids of the pBS series (pBSSK+, pBSSK- and others; Stratagene, LaJolla, USA), or cosmids as SuperCosl (Stratagene, LaJolla, USA) or Lorist6 (Gibson, T.J., Rosenthal A. and Waterson, R.H. (1987) Gene 53:283-286. Gene libraries specifically for use in C. glutamicum may be constructed using plasmid pSL109 (Lee, and A.J. Sinskey (1994) J. Microbiol. Biotechnol. 4:256-263).

20 Example 3: DNA Sequencing and Computational Functional Analysis S. Genomic libraries as described in Example 2 were used for DNA sequencing according to standard methods, in particular by the chain termination method using AB1377 sequencing machines (see Fleischman, R.D. et al.

(1995) "Whole-genome Random Sequencing and Assembly of Haemophilus Influenzae Rd., Science, 269:496-512). Sequencing primers with the following nucleotide sequences were used: 5'-GGAAACAGTATGACCATG-3' (SEQ ID NO.

1157) or 5'-GTAAAACGACGGCCAGT-3' (SEQ ID NO. 1158).

Example 4: In vivo Mutagenesis In vivo mutagenesis of Corynebacterium glutamicum can be performed by passage of plasmid (or other vector) DNA through E. coli or other microorganisms Bacillus spp. or yeasts such as Saccharomyces cerevisiae) which are impaired in their capabilities to maintain WO 01/00843 PCT/IB00/00923 -134the integrity of their genetic information. Typical mutator strains have mutations in the genes for the DNA repair system mutHLS, mutD, mutT, etc.; for reference, see Rupp, W.D.

(1996) DNA repair mechanisms, in: Escherichia coli and Salmonella, p. 2277-2294, ASM: Washington.) Such strains are well known to those of ordinary skill in the art. The use of such strains is illustrated, for example, in Greener, A. and Callahan, M. (1994) Strategies 7: 32-34.

Example 5: DNA Transfer Between Escherichia coli and Corynebacterium glutamicum Several Corynebacterium and Brevibacterium species contain endogenous plasmids (as pHM1519 or pBL which replicate autonomously (for review see, e.g., Martin, J.F. et al. (1987) Biotechnology, 5:137-146). Shuttle vectors for Escherichia coli and Corynebacterium glutamicum can be readily constructed by using standard vectors for E. coli (Sambrook, J. et al. (1989), "Molecular Cloning: A Laboratory Manual", Cold Spring Harbor Laboratory Press or Ausubel, F.M. et al. (1994) "Current Protocols in Molecular Biology", John Wiley Sons) to which a origin or replication for and a suitable marker from Corynebacterium glutamicum is added. Such origins of replication are preferably taken from endogenous plasmids isolated from Corynebacterium and Brevibacterium species. Of particular use as transformation markers for these species are genes for kanamycin resistance (such as those derived from the Tn5 or Tn903 transposons) or chloramphenicol (Winnacker, E.L. (1987) "From Genes to Clones Introduction to Gene Technology, VCH, Weinheim). There are numerous examples in the literature of the construction of a wide variety of shuttle vectors which replicate in both E.

coli and C. glutamicum, and which can be used for several purposes, including gene overexpression (for reference, see Yoshihama, M. et al. (1985) J. Bacteriol. 162:591-597, Martin J.F. et al. (1987) Biotechnology, 5:137-146 and Eikmanns, B.J. et al. (1991) Gene, 102:93-98).

Using standard methods, it is possible to clone a gene of interest into one of the shuttle vectors described above and to introduce such a hybrid vectors into strains of Corynebacterium glutamicum. Transformation of C. glutamicum can be achieved by protoplast transformation (Kastsumata, R. et al. (1984) J. Bacteriol. 159306-311), electroporation (Liebl, E. et al. (1989) FEMS Microbiol. Letters, 53:399-303) and in cases where special vectors are used, also by conjugation (as described e.g. in Schafer, A et al.

WO 01/00843 PCT/IB00/00923 -135- (1990) J. Bacteriol. 172:1663-1666). It is also possible to transfer the shuttle vectors for C glutamicum to E. coli by preparing plasmid DNA from C. glutamicum (using standard methods well-known in the art) and transforming it into E. coli. This transformation step can be performed using standard methods, but it is advantageous to use an Mcr-deficient E. coli strain, such as NM522 (Gough Murray (1983) J. Mol. Biol. 166:1-19).

Genes may be overexpressed in C. glutamicum strains using plasmids which comprise pCGI Patent No. 4,617,267) or fragments thereof, and optionally the gene for kanamycin resistance from TN903 (Grindley, N.D. and Joyce, C.M. (1980) Proc. Natl. Acad. Sci. USA 77(12): 7176-7180). In addition, genes may be overexpressed in C. glutamicum strains using plasmid pSL109 (Lee, and A. J.

Sinskey (1994) J. Microbiol. Biotechnol. 4: 256-263).

SAside from the use of replicative plasmids, gene overexpression can also be achieved by integration into the genome. Genomic integration in C. glutamicum or other Corynebacterium or Brevibacterium species may be accomplished by well-known methods, such as homologous recombination with genomic region(s), restriction endonuclease mediated integration (REMI) (see, DE Patent 19823834), or through the use of transposons. It is also possible to modulate the activity of a gene of interest by modifying the regulatory regions a promoter, a repressor, and/or an enhancer) by sequence modification, insertion, or deletion using site-directed methods (such as homologous recombination) or methods based on random events (such as transposon mutagenesis or REMI). Nucleic acid sequences which function as transcriptional terminators may also be inserted 3' to the coding region of one or more genes of the invention; such terminators are well-known in the art and are described, for example, in Winnacker, E.L. (1987) From Genes to Clones Introduction to Gene Technology. VCH: Weinheim.

Example 6: Assessment of the Expression of the Mutant Protein Observations of the activity of a mutated protein in a transformed host cell rely on the fact that the mutant protein is expressed in a similar fashion and in a similar quantity to that of the wild-type protein. A useful method to ascertain the level of transcription of the mutant gene (an indicator of the amount of mRNA available for translation to the gene product) is to perform a Northern blot (for reference see, for example, Ausubel et al.

WO 01/00843 PCT/IB00/00923 -136- (1988) Current Protocols in Molecular Biology, Wiley: New York), in which a primer designed to bind to the gene of interest is labeled with a detectable tag (usually radioactive or chemiluminescent), such that when the total RNA of a culture of the organism is extracted, run on gel, transferred to a stable matrix and incubated with this probe, the binding and quantity of binding of the probe indicates the presence and also the quantity of mRNA for this gene. This information is evidence of the degree of transcription of the mutant gene. Total cellular RNA can be prepared from Corynebacterium glutamicum by several methods, all well-known in the art, such as that described in Bormann, E.R. et al.

(1992) Mol. Microbiol. 6: 317-326.

To assess the presence or relative quantity of protein translated from this mRNA, standard techniques, such as a Western blot, may be employed (see, for example, Ausubel et al. (1988) Current Protocols in Molecular Biology, Wiley: New York). In this process, total cellular proteins are extracted, separated by gel electrophoresis, transferred to a matrix such as nitrocellulose, and incubated with a probe, such as an antibody, which specifically binds to the desired protein. This probe is generally tagged with a chemiluminescent or colorimetric label which may be readily detected. The presence and quantity of label observed indicates the presence and quantity of the desired mutant protein present in the cell.

Example 7: Growth of Genetically Modified Corynebacterium glutamicum Media and Culture Conditions Genetically modified Corynebacteria are cultured in synthetic or natural growth media. A number of different growth media for Corynebacteria are both well-known and readily available (Lieb et al. (1989) Appl. Microbiol. Biotechnol., 32:205-210; von der Osten et al. (1998) Biotechnology Letters, 11:11-16; Patent DE 4,120,867; Liebl (1992) "The Genus Corynebacterium, in: The Procaryotes, Volume II, Balows, A. et al., eds.

Springer-Verlag). These media consist of one or more carbon sources, nitrogen sources, inorganic salts, vitamins and trace elements. Preferred carbon sources are sugars, such as mono-, di-, or polysaccharides. For example, glucose, fructose, mannose, galactose, ribose, sorbose, ribulose, lactose, maltose, sucrose, raffinose, starch or cellulose serve as very good carbon sources. It is also possible to supply sugar to the media via complex compounds such as molasses or other by-products from sugar refinement. It can also be WO 01/00843 PCT/IB00/00923 -137advantageous to supply mixtures of different carbon sources. Other possible carbon sources are alcohols and organic acids, such as methanol, ethanol, acetic acid or lactic acid. Nitrogen sources are usually organic or inorganic nitrogen compounds, or materials which contain these compounds. Exemplary nitrogen sources include ammonia gas or ammonia salts, such as NH 4 CI or NIHOH, nitrates, urea, amino acids or complex nitrogen sources like corn steep liquor, soy bean flour, soy bean protein, yeast extract, meat extract and others.

Inorganic salt compounds which may be included in the media include the chloride-, phosphorous- or sulfate- salts of calcium, magnesium, sodium, cobalt, molybdenum, potassium, manganese, zinc, copper and iron. Chelating compounds can be added to the medium to keep the metal ions in solution. Particularly useful chelating compounds include dihydroxyphenols, like catechol or protocatechuate, or organic acids, such as citric acid. It is typical for the media to also contain other growth factors, such as vitamins or growth promoters, examples of which include biotin, riboflavin, thiamin, folic acid, nicotinic acid, pantothenate and pyridoxin. Growth factors and salts frequently originate from complex media components such as yeast extract, molasses, corn steep liquor and others. The exact composition of the media compounds depends strongly on the immediate experiment and is individually decided for each specific case. Information about media optimization is available in the textbook "Applied Microbiol. Physiology, A Practical Approach (eds. P.M. Rhodes, P.F. Stanbury, IRL Press (1997) pp. 53-73, ISBN 0 19 963577 It is also possible to select growth media from commercial suppliers, like standard 1 (Merck) or BHI (grain heart infusion, DIFCO) or others.

All medium components are sterilized, either by heat (20 minutes at 1.5 bar and 121'C) or by sterile filtration. The components can either be sterilized together or, if necessary, separately. All media components can be present at the beginning of growth, or they can optionally be added continuously or batchwise.

Culture conditions are defined separately for each experiment. The temperature should be in a range between 15*C and 45C. The temperature can be kept constant or can be altered during the experiment. The pH of the medium should be in the range of 5 to 8.5, preferably around 7.0, and can be maintained by the addition of buffers to the media.

An exemplary buffer for this purpose is a potassium phosphate buffer. Synthetic buffers such as MOPS, HEPES, ACES and others can alternatively or simultaneously be used. It WO 01/00843 PCT/IB00/00923 138 is also possible to maintain a constant culture pH through the addition of NaOH or NHIOH during growth. If complex medium components such as yeast extract are utilized, the necessity for additional buffers may be reduced, due to the fact that many complex compounds have high buffer capacities. If a fermentor is utilized for culturing the microorganisms, the pH can also be controlled using gaseous ammonia.

The incubation time is usually in a range from several hours to several days. This time is selected in order to permit the maximal amount of product to accumulate in the broth. The disclosed growth experiments can be carried out in a variety of vessels, such as microtiter plates, glass tubes, glass flasks or glass or metal fermentors of different sizes.

For screening a large number of clones, the microorganisms should be cultured in microtiter plates, glass tubes or shake flasks, either with or without baffles. Preferably 100 ml shake flasks are used, filled with 10% (by volume) of the required growth medium. The flasks should be shaken on a rotary shaker (amplitude 25 mm) using a speed-range of 100 300 rpm. Evaporation losses can be diminished by the maintenance of a humid atmosphere; alternatively, a mathematical correction for evaporation losses should be performed.

If genetically modified clones are tested, an unmodified control clone or a control clone containing the basic plasmid without any insert should also be tested. The medium is inoculated to an OD 600 of 0.5 1.5 using cells grown on agar plates, such as CM plates (10 g/l glucose, 2,5 g/l NaCI, 2 g/l urea, 10 g/l polypeptone, 5 g/l yeast extract, 5 g/l meat extract, 22 g/l NaCI, 2 g/l urea, 10 g/l polypeptone, 5 g/l yeast extract, 5 g/l meat extract, 22 g/l agar, pH 6.8 with 2M NaOH) that had been incubated at 30'C. Inoculation of the media is accomplished by either introduction of a saline suspension of C. glutamicum cells from CM plates or addition of a liquid preculture of this bacterium.

Example 8 In vitro Analysis of the Function of Mutant Proteins The determination of activities and kinetic parameters of enzymes is well established in the art. Experiments to determine the activity of any given altered enzyme must be tailored to the specific activity of the wild-type enzyme, which is well within the ability of one of ordinary skill in the art. Overviews about enzymes in general, as well as specific details concerning structure, kinetics, principles, methods, applications and examples for the determination of many enzyme activities may be WO 01/00843 PCT/IB00/00923 -139found, for example, in the following references: Dixon, and Webb, (1979) Enzymes. Longmans: London; Fersht, (1985) Enzyme Structure and Mechanism.

Freeman: New York; Walsh, (1979) Enzymatic Reaction Mechanisms. Freeman: San Francisco; Price, Stevens, L. (1982) Fundamentals of Enzymology. Oxford Univ.

Press: Oxford; Boyer, ed. (1983) The Enzymes, 3 r d ed. Academic Press: New York; Bisswanger, (1994) Enzymkinetik, 2 nd ed. VCH: Weinheim (ISBN 3527300325); Bergmeyer, Bergmeyer, Gral1, eds. (1983-1986) Methods of Enzymatic Analysis, 3 r ed., vol. I-XII, Verlag Chemie: Weinheim; and Ullmann's Encyclopedia of Industrial Chemistry (1987) vol. A9, "Enzymes". VCH: Weinheim, p.

352-363.

The activity of proteins which bind to DNA can be measured by several wellestablished methods, such as DNA band-shift assays (also called gel retardation assays).

The effect of such proteins on the expression of other molecules can be measured using reporter gene assays (such as that described in Kolmar, H. et al. (1995) EMBO J. 14: 3895-3904 and references cited therein). Reporter gene test systems are well known and established for applications in both pro- and eukaryotic cells, using enzymes such as beta-galactosidase, green fluorescent protein, and several others.

The determination of activity of membrane-transport proteins can be performed according to techniques such as those described in Gennis, R.B. (1989) "Pores, Channels and Transporters", in Biomembranes, Molecular Structure and Function, Springer: Heidelberg, p. 85-137; 199-234; and 270-322.

Example 9: Analysis of Impact of Mutant Protein on the Production of the Desired Product The effect of the genetic modification in C. glutamicum on production of a desired compound (such as an amino acid) can be assessed by growing the modified microorganism under suitable conditions (such as those described above) and analyzing the medium and/or the cellular component for increased production of the desired product an amino acid). Such analysis techniques are well known to one of ordinary skill in the art, and include spectroscopy, thin layer chromatography, staining methods of various kinds, enzymatic and microbiological methods, and analytical chromatography such as high performance liquid chromatography (see, for example, WO 01/00843 PCT/IB00/00923 -140- Ullman, Encyclopedia of Industrial Chemistry, vol. A2, p. 89-90 and p. 443-613, VCH: Weinheim (1985); Fallon, A. et al., (1987) "Applications of HPLC in Biochemistry" in: Laboratory Techniques in Biochemistry and.Molecular Biology, vol. 17; Rehm et al.

(1993) Biotechnology, vol. 3, Chapter III: "Product recovery and purification", page 469-714, VCH: Weinheim; Belter, P.A. et al. (1988) Bioseparations: downstream processing for biotechnology, John Wiley and Sons; Kennedy, J.F. and Cabral, J.M.S.

(1992) Recovery processes for biological materials, John Wiley and Sons; Shaeiwitz, J.A. and Henry, J.D. (1988) Biochemical separations, in: Ulmann's Encyclopedia of Industrial Chemistry, vol. B3, Chapter 11, page 1-27, VCH: Weinheim; and Dechow, F.J. (1989) Separation and purification techniques in biotechnology, Noyes Publications.) In addition to the measurement of the final product of fermentation, it is also possible to analyze other components of the metabolic pathways utilized for the production of the desired compound, such as intermediates and side-products, to determine the overall efficiency of production of the compound. Analysis methods include measurements of nutrient levels in the medium sugars, hydrocarbons, nitrogen sources, phosphate, and other ions), measurements ofbiomass composition and growth, analysis of the production of common metabolites of biosynthetic pathways, and measurement of gasses produced during fermentation. Standard methods for these measurements are outlined in Applied Microbial Physiology, A Practical Approach, P.M. Rhodes and P.F. Stanbury, eds., IRL Press, p. 103-129; 131-163; and 165-192 (ISBN: 0199635773) and references cited therein.

Example 10: Purification of the Desired Product from C. glutamicum Culture Recovery of the desired product from the C. glutamicum cells or supernatant of the above-described culture can be performed by various methods well known in the art.

If the desired product is not secreted from the cells, the cells can be harvested from the culture by low-speed centrifugation, the cells can be lysed by standard techniques, such as mechanical force or sonication. The cellular debris is removed by centrifugation, and the supernatant fraction containing the soluble proteins is retained for further purification of the desired compound. If the product is secreted from the C. glutamicum WO 01/00843 PCT/IB00/00923 141 cells, then the cells are removed from the culture by low-speed centrifugation, and the supernate fraction is retained for further purification.

The supernatant fraction from either purification method is subjected to chromatography with a suitable resin, in which the desired molecule is either retained on a chromatography resin while many of the impurities in the sample are not, or where the impurities are retained by the resin while the sample is not. Such chromatography steps may be repeated as necessary, using the same or different chromatography resins. One of ordinary skill in the art would be well-versed in the selection of appropriate chromatography resins and in their most efficacious application for a particular molecule to be purified. The purified product may be concentrated by filtration or ultrafiltration, and stored at a temperature at which the stability of the product is maximized.

There are a wide array of purification methods known to the art and the preceding method of purification is not meant to be limiting. Such purification techniques are described, for example, in Bailey, J.E. Ollis, D.F. Biochemical Engineering Fundamentals, McGraw-Hill: New York (1986).

The identity and purity of the isolated compounds may be assessed by techniques standard in the art. These include high-performance liquid chromatography (HPLC), spectroscopic methods, staining methods, thin layer chromatography, NIRS, enzymatic assay, or microbiologically. Such analysis methods are reviewed in: Patek et al. (1994) Appl. Environ. Microbiol. 60: 133-140; Malakhova et al. (1996) Biotekhnologiya 11: 27- 32; and Schmidt et al. (1998) Bioprocess Engineer. 19: 67-70. Ulmann's Encyclopedia of Industrial Chemistry, (1996) vol. A27, VCH: Weinheim, p. 89-90, p. 521-540, p. 540- 547, p. 559-566, 575-581 and p. 581-587; Michal, G. (1999) Biochemical Pathways: An Atlas of Biochemistry and Molecular Biology, John Wiley and Sons; Fallon, A. et al.

(1987) Applications of HPLC in Biochemistry in: Laboratory Techniques in Biochemistry and Molecular Biology, vol. 17.

Example 11: Analysis of the Gene Sequences of the Invention The comparison of sequences and determination of percent homology between two sequences are art-known techniques, and can be accomplished using a mathematical algorithm, such as the algorithm of Karlin and Altschul (1990) Proc. Natl. Acad Sci.

USA 87:2264-68, modified as in Karlin and Altschul (1993) Proc. Natl. Acad Sci. USA WO 01/00843 PCT/IB00/00923 142 90:5873-77. Such an algorithm is incorporated into the NBLAST and XBLAST programs (version 2.0) of Altschul, et al. (1990) J. Mol. Biol. 215:403-10. BLAST nucleotide searches can be performed with the NBLAST program, score 100, wordlength 12 to obtain nucleotide sequences homologous to MP nucleic acid molecules of the invention. BLAST protein searches can be performed with the XBLAST program, score 50, wordlength 3 to obtain amino acid sequences homologous to MP protein molecules of the invention. To obtain gapped alignments for comparison purposes, Gapped BLAST can be utilized as described in Altschul et al., (1997) Nucleic Acids Res. 25(17):3389-3402. When utilizing BLAST and Gapped BLAST programs, one of ordinary skill in the art will know how to optimize the parameters of the program XBLAST and NBLAST) for the specific sequence being analyzed.

Another example of a mathematical algorithm utilized for the comparison of sequences is the algorithm of Meyers and Miller ((1988) Comput. Appl. Biosci. 4: 11- 17). Such an algorithm is incorporated into the ALIGN program (version 2.0) which is part of the GCG sequence alignment software package. When utilizing the ALIGN program for comparing amino acid sequences, a PAM120 weight residue table, a gap length penalty of 12, and a gap penalty of 4 can be used. Additional algorithms for sequence analysis are known in the art, and include ADVANCE and ADAM. described in Torelli and Robotti (1994) Comput. Appl. Biosci. 10:3-5; and FASTA, described in Pearson and Lipman (1988) P.N.A.S. 85:2444-8.

The percent homology between two amino acid sequences can also be accomplished using the GAP program in the GCG software package (available at http://www.gcg.com), using either a Blosum 62 matrix or a PAM250 matrix, and a gap weight of 12, 10, 8, 6, or 4 and a length weight of 2, 3, or 4. The percent homology between two nucleic acid sequences can be accomplished using the GAP program in the GCG software package, using standard parameters, such as a gap weight of 50 and a length weight of 3.

A comparative analysis of the gene sequences of the invention with those present in Genbank has been performed using techniques known in the art (see, Bexevanis and Ouellette, eds. (1998) Bioinformatics: A Practical Guide to the Analysis of Genes and Proteins. John Wiley and Sons: New York). The gene sequences of the invention WO 01/00843 PCT/IB00/00923 143were compared to genes present in Genbank in a three-step process. In a first step, a BLASTN analysis a local alignment analysis) was performed for each of the sequences of the invention against the nucleotide sequences present in Genbank, and the top 500 hits were retained for'further analysis. A subsequent FASTA search a combined local and global alignment analysis, in which limited regions of the sequences are aligned) was performed on these 500 hits. Each gene sequence of the invention was subsequently globally aligned to each of the top three FASTA hits, using the GAP program in the GCG software package (using standard parameters). In order to obtain correct results, the length of the sequences extracted from Genbank were adjusted to the length of the query sequences by methods well-known in the art. The results of this analysis are set forth in Table 4. The resulting data is identical to that which would have been obtained had a GAP (global) analysis alone been performed on each of the genes of the invention in comparison with each of the references in Genbank, but required significantly reduced computational time as compared to such a database-wide GAP (global) analysis. Sequences of the invention for which no alignments above the cutoff values were obtained are indicated on Table 4 by the absence of alignment information.

It will further be understood by one of ordinary skill in the art that the GAP alignment homology percentages set forth in Table 4 under the heading homology (GAP)" are listed in the European numerical format, wherein a represents a decimal point. For example, a value of "40,345" in this column represents "40.345%".

Example 12: Construction and Operation of DNA Microarrays The sequences of the invention may additionally be used in the construction and application of DNA microarrays (the design, methodology, and uses of DNA arrays are well known in the art, and are described, for example, in Schena, M. et al. (1995) Science 270: 467-470; Wodicka, L. et al. (1997) Nature Biotechnology 15: 1359-1367; DeSaizieu, A. et al. (1998) Nature Biotechnology 16: 45-48; and DeRisi, J.L. et al.

(1997) Science 278: 680-686).

DNA microarrays are solid or flexible supports consisting of nitrocellulose, nylon, glass, silicone, or other materials. Nucleic acid molecules may be attached to the surface in an ordered manner. After appropriate labeling, other nucleic acids or nucleic acid mixtures can be hybridized to the immobilized nucleic acid molecules, and the label WO 01/00843 PCT/IB00/00923 -144may be used to monitor and measure the individual signal intensities of the hybridized molecules at defined regions. This methodology allows the simultaneous quantification of the relative or absolute amount of all or selected nucleic acids in the applied nucleic acid sample or mixture. DNA microarrays, therefore, permit an analysis of the expression of multiple (as many as 6800 or more) nucleic acids in parallel (see, e.g., Schena, M. (1996) BioEssays 18(5): 427-431).

The sequences of the invention may be used to design oligonucleotide primers which are able to amplify defined regions of one or more C glutamicum genes by a nucleic acid amplification reaction such as the polymerase chain reaction. The choice and design of the 5' or 3' oligonucleotide primers or of appropriate linkers allows the covalent attachment of the resulting PCR products to the surface of a support medium described above (and also described, for example, Schena, M. et al. (1995) Science 270: 467-470).

Nucleic acid microarrays may also be constructed by in situ oligonucleotide synthesis as described by Wodicka, L. et al. (1997) Nature Biotechnology 15: 1359- 1367. By photolithographic methods, precisely defined regions of the matrix are exposed to light. Protective groups which are photolabile are thereby activated and undergo nucleotide addition, whereas regions that are masked from light do not undergo any modification. Subsequent cycles of protection and light activation permit the synthesis of different oligonucleotides at defined positions. Small, defined regions of the genes of the invention may be synthesized on microarrays by solid phase oligonucleotide synthesis.

The nucleic acid molecules of the invention present in a sample or mixture of nucleotides may be hybridized to the microarrays. These nucleic acid molecules can be labeled according to standard methods. In brief, nucleic acid molecules mRNA molecules or DNA molecules) are labeled by the incorporation of isotopically or fluorescently labeled nucleotides, during reverse transcription or DNA synthesis.

Hybridization of labeled nucleic acids to microarrays is described in Schena, M. et al. (1995) supra; Wodicka, L. et al. (1997), supra; and DeSaizieu A. et al. (1998), supra). The detection and quantification of the hybridized molecule are tailored to the specific incorporated label. Radioactive labels can be detected, for example, as WO 01/00843 PCT/IB00/00923 -145described in Schena, M. et al. (1995) supra) and fluorescent labels may be detected, for example, by the method of Shalon et al. (1996) Genome Research 6: 639-645).

The application of the sequences of the invention to DNA microarray technology, as described above, permits comparative analyses of different strains of C.

glutamicum or other Corynebacteria. For example, studies of inter-strain variations based on individual transcript profiles and the identification of genes that are important for specific and/or desired strain properties such as pathogenicity, productivity and stress tolerance are facilitated by nucleic acid array methodologies. Also, comparisons of the profile of expression of genes of the invention during the course of a fermentation reaction are possible using nucleic acid array technology.

Example 13: Analysis of the Dynamics of Cellular Protein Populations (Proteomics) The genes, compositions, and methods of the invention may be applied to study the interactions and dynamics of populations of proteins, termed 'proteomics'. Protein populations of interest include, but are not limited to, the total protein population of C.

glutamicum in comparison with the protein populations of other organisms), those proteins which are active under specific environmental or metabolic conditions during fermentation, at high or low temperature, or at high or low pH), or those proteins which are active during specific phases of growth and development.

Protein populations can be analyzed by various well-known techniques, such as gel electrophoresis. Cellular proteins may be obtained, for example, by lysis or extraction, and may be separated from one another using a variety of electrophoretic techniques. Sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) separates proteins largely on the basis of their molecular weight. Isoelectric focusing polyacrylamide gel electrophoresis (IEF-PAGE) separates proteins by their isoelectric point (which reflects not only the amino acid sequence but also posttranslational modifications of the protein). Another, more preferred method of protein analysis is the consecutive combination of both IEF-PAGE and SDS-PAGE, known as 2-D-gel electrophoresis (described, for example, in Hermann et al. (1998) Electrophoresis 19: 3217-3221; Fountoulakis et al. (1998) Electrophoresis 19: 1193-1202; Langen et al.

(1997) Electrophoresis 18: 1184-1192; Antelmann et al. (1997) Electrophoresis 18: WO 01/00843 PCTIB00/00923 -146- 1451-1463). Other separation techniques may also be utilized for protein separation, such as capillary gel electrophoresis; such techniques are well known in the art.

Proteins separated by these methodologies can be visualized by standard techniques, such as by staining or labeling. Suitable stains are known in the art, and include Coomassie Brilliant Blue, silver stain, or fluorescent dyes such as Sypro Ruby (Molecular Probes). The inclusion of radioactively labeled amino acids or other protein precursors "S-methionine, 35S-cysteine, 14C-labelled amino acids, 5 N-amino acids, 15 N0 3 or 5 NI-L4 or 1 3 C-labelled amino acids) in the medium of C. glutamicum permits the labeling of proteins from these cells prior to their separation. Similarly, fluorescent labels may be employed. These labeled proteins can be extracted, isolated and separated according to the previously described techniques.

Proteins visualized by these techniques can be further analyzed by measuring the amount of dye or label used. The amount of a given protein can be determined quantitatively using, for example, optical methods and can be compared to the amount of other proteins in the same gel or in other gels. Comparisons of proteins on gels can be made, for example, by optical comparison, by spectroscopy, by image scanning and analysis of gels, or through the use of photographic films and screens. Such techniques are well-known in the art.

To determine the identity of any given protein, direct sequencing or other standard techniques may be employed. For example, N- and/or C-terminal amino acid sequencing (such as Edman degradation) may be used, as may mass spectrometry (in particular MALDI or ESI techniques (see, Langen et al. (1997) Electrophoresis 18: 1184-1192)). The protein sequences provided herein can be used for the identification of C. glutamicum proteins by these techniques.

The information obtained by these methods can be used to compare patterns of protein presence, activity, or modification between different samples from various biological conditions different organisms, time points of fermentation, media conditions, or different biotopes, among others). Data obtained from such experiments alone, or in combination with other techniques, can be used for various applications, such as to compare the behavior of various organisms in a given metabolic) situation, to increase the productivity of strains which produce fine chemicals or to increase the efficiency of the production of fine chemicals.

06/10 '05 THU 16:03 FAX 61 2 9888 7600 WATERMARK t1015 147

EQUIVALENTS

Those of ordinary skill in the art will recognize, or will be able to ascertain using no more than routine experimentation, many equivalents to the specific embodiments of the invention described herein. Such equivalents are intended to be encompassed by the following claims.

Comprises/comprising and grammatical variations thereof when used in this specification are to be taken to specify the presence of stated features, integers, steps or components or groups thereof, but do not preclude the presence or addition of one or more other features, integers, steps, components or groups thereof.

S

COMS ID No: SBMI-01677437 Received by IP Australia: Time 16:10 Date 2005-10-06 EDITORIAL NOTE APPLICATION NUMBER 54213-00 The following Sequence Listing pages 1 to 1582 are part of the description. The claims pages follow on pages "148" to "152".

WO 01/00843 WO 0100843PCTIBOO/00923 SEQUENCE LISTING <110> BASF Aktiengesellschaft <120> CORYNEBACTERIUM GLUTAMICUM GENES ENCODING METABOLIC PATHWAY PROTEINS <130> BGI-121CPPC <140> <141> <160> 1156 <210> <211> <212> <213> <220> <221> <222> <223> 1 948

DNA

Corynebac terium glutamicum

CDS

(101)..(925) RXA02 229 <400> 1 gctggttcaa cagagaccac cgcgtgtcct gggtcgacgc ctctggcgat cccaccgcac aagccttgga gattttgggt ctacaatagc gagggtgaat ttg acc atc ccc ttt Leu Thr Ile Pro Phe gcc aaa ggc cac Ala Lys Gly His gcc Ala acc gaa aac gac ttc atc atc atc ccc Thr Giu Asn Asp Phe Ile Ile Ile Pro gat gag Asp Glu gat gcg cgc cta gat tta act cca gaa atg gtg gtc acg ctg tgt gac Asp Ala Arg Leu Asp Leu Thr Pro.Glu Met Val Val Thr Leu Cys Asp 30 cgc cgc gcc Arg Arg Ala ggg atc ggt gct gat ggt atc ctc cgc Gly Ile Gly Ala Asp Gly Ile Leu Arg 45 gtg Val1 gtt aaa gct Val Lys Ala gca gac gta gaa ggc tcc acg gtc gac cca tcg Ala Asp Val Glu Gly Ser Thr Val Asp Pro Ser tgg ttc atg gat Trp, Phe Met Asp tac Tyr cgc aac gcc gat Arg Asn Ala Asp tct ttg gct gaa Ser Leu Ala Glu tgc ggc aat ggt Cys Gly Asn Gly cgc ctg ttc gcg Arg Leu Phe Ala cac His tgg ctg tac tcc cgc Trp Leu Tyr Ser Arg .95 ggt ctt gtt gat Gly Leu Val Asp aat acg Asn Thr 100 agc ttt gat Ser Phe Asp cag gca gat Gin Ala Asp 120 ggt acc cgc gcc ggt gtc cgc. cac gtt Gly Thr Arg Ala Gly Val Arg His Val gat att ttg Asp Ile Leu 115 ggc atc cct Gly Ile Pro caa cat tct gcg cag gtc cgc gtt gat Gin His Ser Ala Gin Val Arg Val Asp 125 atg Met 130 gac gtc acg gga tta tcc acc tgc gac atc aac ggc caa gta ttc gct Asp Val Thr Gly Leu Ser Thr Cys Asp Ile Asn Gly Gin Val Phe Ala WO 01/00843 PCTIBOOIOO923 135 140 145 ggc ctt ggc gtt gat atg ggt aac cca cac cta gcg tgc gtt gtg ccg 595 Gly Leu Gly Val Asp Met Gly Asn Pro His Leu Ala Cys Val Val Pro 150 155 160 165 ggc tta agt gcg tcg gct ctt gcc gat atg gaa ctg cgc gca cct acg 643 Gly Leu Ser Ala Ser Ala Leu Ala Asp Met Giu Leu Arg Ala Pro Thr 170 175 180 ttt gat cag gaa ttc ttc ccc cac ggt gtg aac gta gaa atc gtc aca 691 Phe Asp Gin Giu Phe Phe Pro His Gly Val Asn Val Giu Ile Val Thr 185 190 195 gaa tta gaa gat gac gca gta tcg atg cgc gtg tgg gaa cgc gga gtg 739 Giu Leu Giu Asp Asp Ala Val Ser Met Arg Val Trp Giu Arg Gly Val 200 205 210 ggc gaa acc cgc tcc tgt ggc acg gga acc gtt gct gca gcg tgt gct 787 Gly Giu Thr Arg Ser Cys Gly Thr Gly Thr Val Ala Ala Ala Cys Ala 215 220 225 gct tta gct gat gct gga ttg gga gaa ggc aca gct aaa gtg tgc gtt 835 Ala Leu Ala Asp Ala Gly Leu Gly Giu Gly Thr Ala Lys Val Cys Val 230 235 240 245 cca cgt ggg gaa gta gaa gtc cag atc ttt gac gac ggc tcc aca ctc 883 Pro Arg Gly Giu Val Giu Val Gin Ile Phe Asp Asp Gly Ser Thr Leu 250 255 260 acc ggc cca agc gcc atc atc gca ctc ggt gag gtg cag atc 925 Thr Gly Pro Ser Ala Ile Ile Ala Leu Gly Giu Val Gin Ile 265 270 275 taagattcgc gattgtagtt Cgg 948 <210> 2 <211> 275 <212> PRT <213> Corynebacterium giutamicum <400> 2 Leu Thr Ilie Pro Phe Ala Lys Gly His Ala Thr Giu Asn ASP Phe Ile 1 5 10 Ile Ilie Pro Asp Glu Asp Ala Arg Leu Asp Leu Thr Pro Giu Met Val 25 Vai Thr Leu Cys Asp Arg Arg Ala Gly Ile Gly Ala Asp Giy Ile Leu 40 Arg Val Val Lys Ala Aia Asp Val Giu Gly Ser Thr Val Asp Pro Ser 55 Leu Trp, Phe Met Asp Tyr Arg Asn Ala Asp Giy Ser Leu Aia Glu Met 70 75 Cys Giy Asn Gly Val Arg Leu Phe Ala His Trp, Leu Tyr Ser Arg Gly 90 WO 01/00843 PCTJ1BO0100923 Gly Val Arg 110 Leu His Asp Gly 145 Ala Leu Val1 Trp Ala 225 Ala Val Val Met 130 Gin Cys Arg Glu Giu 210 Ala Lys Asp Asn T 100 Asp Ile L 115 Gly Ile P Val Phe A Val Val P 1 Ala Pro T 180 Ilie Val 195 Arg Gly V Ala Cys A~ Val Cys V 2 hr Ser Phe Asp Ile Gly Thr Arg Ala 105 eu ro la ro 65 'hr hr al la 'al 45 Gin Asp Gly i50 Gly Phe Glu Gly Ala 230 Pro Ala Val 135 Leu Leu Asp Leu Giu 215 Leu Arg Asp 120 Thr Gly Ser Gin Giu 200 Thr Aila Gly Gin Gly Val Ala Giu 185 Asp Arg Asp Glu Ser Ser Met 155 Ala Phe Ala Cys Gly 235 Giu Al a Thr 140 Gly Leu Pro Val Gly 220 Leu Val Gin 125 Cys Asn Ala His Ser 205 Thr Gly Gln Val Asp Pro Asp Gly 190 Met Giy Giu Ile Arg Ile His Met 175 Val Arg Thr Gly Phe 255

ASP

Val Gly Gin Ser Thr 260 Ile 275 Leu Thr Gly Pro Ser Ala Ile Ile Ala Leu Gly Giu <210> 3 <211> 1491 <212> DNA <213> Corynebacteriun glutanicun <22 0> <221> CDS <222> (101)..(1468) <223> RXS02970 <400> 3 aaccgacaaa acagccgttc acgtgctaaa gCagctcggc ttgatctagg gtgaggtgag ttatttaaag acttcataat attttgggga gtgaactggt ttg gca ttg aag ggt Leu Ala Leu Lys Gly 1 tac acc aac ttt gac ggt gaa ttc atc gaa ttc gga tct gtg caa gca Tyr Thr Asn Phe Asp Gly Giu Phe Ile Giu Phe Giy Ser Val Gln Ala 15 aaa gaa gag gaa aaa cgg gca ttc gac aac gat cgc gcg cac gtt ttc Lys Glu Glu Giu Lys Arg Ala Phe Asp Asn Asp Arg Ala His Val Phe 30 WO 01/00843 WO 0100843PCT/IBOO/00923 cac His gcc Ala atg Met tta Leu ccg Pro tcg Ser gcc Ala cgc Arg 150 tcc Ser ac c Thr tca Ser cac His atc Ile 230 gca Ala tcc Ser gaa Giu ggt Gly gtt Val1 gcc Ala atg Met gac Asp 135 aac Asn gcg Ala gac Asp t tc Phe t tg Leu 215 gtc Val1 ggt Gly tgg tcc gcg Trp Ser Ala ggt tcc acg Gly Ser Thr tcc caa ctt Ser Gin Leu gag gcg atC Giu Ala Ile ttc ggc aat Phe Gly Asn 105 gcc cgt ggc Ala Arg Gly 120 gcc atc gag Ala Ilie Giu aaa att ctg Lys Ilie Leu atg atg ctc Met Met Leu 170 cca gat atc Pro Asp Ile 185 ttt gcc acc Phe Ala Thr 200 gaa gat gtc Giu Asp Val ctg gag cca Leu Glu Pro tac tta aat Tyr Leu Asn 250 cag gac aaa atc agc ccc aaa. gta tgg gca gct Gin Asp Lys Ile Ser Pro Lys Val Trp Ala Ala 45 ctg Leu gtc Val1 75 cag Gin gat Asp gaa Giu cac His tcc~ Ser 155 acc Thr t ac Tyr acc Thr atc Ile gtg Val1 235 ggc Gly t ac Tyr 60 tcg Ser cgc Arg gtg Val ttc Phe tcc Ser 140 gca Ala ggc Gly cac His caa Gin gcg Ala 220 gtg Val gtg Val gac Asp gca Ala caa Gin cgc Arg tc c Ser 125 atc Ile tac Tyr gaa Giu ttc Phe gaa Giu 205 ttt Phe gga Gly cgc Arg t tc Phe aac Asn gca Ala tct Ser 110 cac His cgc Arg cgc Arg cac His tgg Trp 190 gaa Glu gaa Giu tca Ser gaa Glu gac Asp tta Leu gcc Ala 95 gat Asp gtg Val atg Met agc Ser cgc Arg 175 gca Ala gaa Glu ggt Gly tca Ser ctt Leu 255 gga Gly ggC Gly ggc Gly 80 cgg Arg gt t Val1 ttt Phe gc t Ala tac Tyr 160 cgc Arg c ca Pro tgc Cys gc t Ala gga Gly 240 tgc Cys t tc Phe aac Asn cac His ttg Leu gc t Ala ttc Phe cgc Arg 145 cac His ctg Leu ttc Phe gaa Glu ggC Gly 225 atc Ilie aac Asn gga Gly gcc Ala aac Asn acc Thr gca Ala acc Thr 130 c tg Leu 99C Gly ggC Gly ctg Leu cgc Arg 210 a tg Met atc Ile aag Lys cgc Arg ttc Phe aac Asn aac Asn aag Lys 115 aac Asn cac His gca Ala aac Asn cac His 195 gca Ala atc Ile ctg Leu cac His acc Thr 275 atc gac Ile Asp cct cga Pro Arg atc aac Ile Asn 100 atc gtg Ile Val Gly Gly acc gga Thr Gly acc gga Thr Gly 165 ccc acc Pro Thr 180 cac tcc His Ser ctc aag Leu Lys gca gcg Ala Ala cca cca Pro Pro 245 ggc atc Gly Ile 260 gga aaa Gly Lys 307 355 403 451 499 547 595 643 691 739 787 835 883 931 ctc ttc atc gcc gac gaa gtc atg gtc Leu Phe Ilie Ala Asp Giu Val Met Val WO 01/00843 ctg ttt gct Leu Phe Ala 280 PCTIBOOIOO923 tac gag cat gct Tyr Glu His Ala acc ttc 1027 Thr Phe 295 gtg atg 1075 Val Met 310 ggc gga 1123 Gly Gly gca gcg 1171 Ala Ala cga ctt 1219 Arg Leu gaa aac 1267 Glu Asn 375 gtg gag 1315 Val Glu 390 ttc aag 1363 Phe Lys atc gcg 1411 Ile Ala gac gcg 1459 Asp Ala gcg ttg 1491 Ala Leu 455 <210> 4 gcc aag ggt gtt aac Ala Lys Gly Val Asn 300 acc caa tca atc cgc Thr Gin Ser Ile Arg ggC Gly 285 gca Al a ga t

ASP

gac gat ttc ASP ASP Phe ggt tac gcc Gly Tyr Ala acc ttc gga Thr Phe Gly cag cca gac atg atc 979 Gin Pro Asp Met Ile 290 cca ctc ggt ggc atc Pro Leu Gly Gly Ile 305 tca gag gca tac tcc Ser Glu Ala Tyr Ser 325 gta gca ccc gcc aag Val Ala Pro Ala Lys 340 att cca cgc gta gct Ile Pro Arg Val Ala ctc acc Leu Thr ctg gag Leu Giu 345 ggc gct Gly Ala 315 320 tac tcc gga cac cca ctt Tyr Ser Gly His Pro Leu 330 335 att tac gcg gaa gga gag Ile Tyr Ala Glu Gly Glu 350 gaa ctg atc gaa cct cgc Giu Leu Ile Giu Pro Arg gca Al a atc Ile 355 ctt cgt gaa cta gcg gaa Leu Arg Glu Leu Ala Giu 370 atc gga ttc ttc tgg gca Ile Gly Phe Phe Tx-p Ala 365 gta gcg atc gct gac Val Ala Ile Ala ASP 380 gtg cgg ggc Val Arg Gly ttc aat gca gac Phe Asn Ala Asp 395 gaa cgc ggc gtg Giu Arg Gly Val 410 gcc Ala 385 act gcc atg gct gcc Thr Ala Met Ala Ala ggt gct gca gaa Gly Ala Ala Giu 405 aac cga ttc cac Asn Arg Phe His tgg ccg atg atc Tx-p Pro Met Ile 415 ggc Gly 420 ccg ccg Pro Pro 425 gtg gaa Val Glu ctg acc acc act gat gac gaa ttg gta gca ctg ctg Leu Thr Thr Thr Asp Asp Glu Leu Val Ala Leu Leu 430 435 gct gca gcc caa gct gtc gag ctg acc ttc gct ggg Ala Ala Ala Gin Ala Val Giu Leu Thr Phe Ala Gly 445 450 taagttttct agataacaag gcc WO 01/00843 WO 0100843PCTIBOO/00923 <211> 456 <212> PRT <213> Corynebacteriun giutanicun <400> 4 Leu Ala Leu Lys Gly Tyr Thr Asn Phe Asp Gly Glu Phe Ilie Glu Phe 1 Gly Arg Lys Asn His Leu Ala Phe Arg 145 His Leu Phe Giu G ly 225 Ile Asn Gly Ser Ala Val Ala Asn Thr Ala Thr 130 Leu Gly Gly Leu Arg 210 Met Ilie Lys Arg Gin Val1 Ala Ilie Pro Ilie 100 Ile Giy Thr Thr Pro 180 His Leu Ala Pro Giy 260 Gly Lys His Aia Met 70 Leu Pro Ser Ala- Arg 150 Ser Thr Ser His Ile 230 Ala Leu Leu Glu Ser Giu 55 Gly Val1 Ala Met Asp 135 Asn Ala Asp Phe Leu 215 Vai G ly Phe Phe Glu 25 Ser Ser Gin Ala Gly 105 Arg Ile Ile Met Asp 185 Ala Asp Giu Leu Ala 265 Tyr 10 Lys Ala Thr Leu Ile Asn Gly Giu Leu Leu 170 Ile Thr Val1 Pro Asn 250 Asp Gi u Gly Arg Gin Leu Val 75 Gin Asp Giu His Ser 155 Thr Tyr Thr Ile Val1 235 Gly Giu His Val Ala Asp Tyr Ser Arg Val Phe Ser 140 Ala Gly His Gin Ala 220 Val1 Val1 Val1 Al a Asn 300

ASP

Ile Phe Asn Ala Ser 110 His Arg Arg His Trp 190 Glu Giu Ser Giu Val1 270 Asp Gly Asn Ser Asp Leu Ala Asp Val Met Ser Arg 175 Ala Giu Gly Ser Loeu 255 Gly Asp Tyr Asp Pro Gly Gly Arg Val Phe Ala Tyr 160 Arg Pro Cys Ala Gly 240 Cys Phe Phe Ala Gin Pro Asp Met Ilie Thr Phe Ala Lys 290 295 WO 01/00843 WO 0100843PCT/IBOO/00923 Pro Leu Gly Gly Ilie Val Met Thr Gin Ser Ile 305 310 315 Ser Glu Ala Tyr Ser Gly Gly Leu Thr Tyr Ser 325 330 Val Ala Pro Ala Lys Ala Ala Leu Glu Ile Tyr 340 345 Ile Pro Arg Val Ala Arg Leu Gly Ala Glu Leu 355 360 Arg Giu Leu Ala Glu Glu Asn Val Ala Ile Ala 370 375 Gly Phe Phe Ti-p Ala Val Glu Phe Asn Ala Asp 385 390 395 Ala Gly Ala Ala Giu Phe Lys Giu Arg Gly Val 405 410 Gly Asn Arg Phe His Ile Ala Pro Pro Leu Thr 420 425 Leu Val Ala Leu Leu Asp Ala Val Giu Ala Ala 435 440 Leu Thr Phe Ala Gry Ala Leu Phe 450 455 <210> <211> 1330 <212> DNA <213> Corynebacterium glutanicuxn <2 <221> CDS <222> (101)..(1330) <223> FRXA01009 <400> aaccgacaaa acagccgttc acgtgctaaa gcagctcggc ttatttaaag acttcataat attttgggga gtgaactggt Asp His Glu Giu 365 Val1 Thr Pro Thr Gin 445 Thr Pro Gly 350 Pro Arg Ala Met Asp 430 Ala tac acc Tyr Thr aaa gaa Lys Giu cac tcc His Ser ttt gac ggt gaa ttc Phe Asp Gly Glu Phe gaa aaa cgg gca ttc Giu Lys Arg Ala Phe tcc gcg cag gac aaa Ser Ala Gin Asp Lys atc gaa ttc Ile Giu Phe 15 gac aac gat Asp Asn Asp 30 atc agc ccc Ile Ser Pro ttgatctagg gtgaggtgag ttg gca ttg aag ggt Leu Ala Leu Lys Gly 1 gga tct gtg caa gca Gly Ser Val Gin Ala cgc gcg cac gtt ttc Arg Aia His Val Phe aaa gta tgg gca gct Lys Val Trp, Ala Ala WO 01/00843 WO 0100843PCTIiBOO/00923 gcc gaa ggt tcc acg ctg tac gac ttc gac ggc aac gcc ttc atc gac Ala Giu Gly Ser Thr Leu Tyr Asp Phe Asp Gly Asn Ala Phe Ile Asp 60 atg Met ggt tcc caa ctt Gly Ser Gin Leu tcg gca aac tta ggc cac aac aac cct Ser Ala Asn Leu Giy His Asn Asn Pro tta gtt gag gcg Leu Val Giu Ala cag cgc caa gca gcc cgg ttg acc aac Gin Arg Gin Ala Ala Arg Leu Thr Asn atc aac Ile Asn 100 ccg gcc ttc Pro Ala Phe tcg atg gcc Ser Met Ala 120 aat gat gtg cgc Asn Asp Vai Arg gat gtt gct gca Asp Val Ala Ala aag atc gtg Lys Ile Vai 115 aac ggc ggc Asn Giy Gly cgt ggc gaa ttc Arg Gly Giu Phe tcc Ser 125 cac gtg ttt ttc His Val Phe Phe gcc gac Aia Asp 135 gcc atc gag cac Aia Ilie Giu His tcc Ser 140 atc cgc atg gct Ile Arg Met Ala ctg cac acc gga Leu His Thr Gly cgc Arg 150 aac aaa att ctg Asn Lys Ilie Leu tcc Ser 155 gca tac cgc agc Ala Tyr Arg Ser cac ggc gca acc His Gly Ala Thr tcc gcg atg atg Ser Ala Met Met ctc Leu 170 acc ggc gaa cac Thr Gly Giu His cgc ctg ggc aac Arg Leu Gly Asn ccc acc Pro Thr 180 acc gac cca Thr Asp Pro tca ttc ttt Ser Phe Phe 200 gat Asp 185 atc tac cac ttc Ile Tyr His Phe gca cca ttc ctg Ala Pro Phe Leu cac cac tcc His His Ser 195 gca ctc aag Ala Leu Lys gcc acc acc caa Ala Thr Thr Gin gaa gaa tgc gaa Giu Giu Cys Giu cgc Arg 210 cac ttg His Leu 215 gaa gat gtc atc Glu ASP Val Ile gcg Al a 220 ttt gaef ggt gct Phe Giu Gly Ala ggc G ly 225 atg atc gca gcg Met Ile Ala Ala gtc ctg gag cca Val Leu Giu Pro gtg Val1 235 gtg gga tca tca Vai Gly Ser Ser atc atc ctg cca Ile Ile Leu Pro gca ggt tac tta Ala Gly Tyr Leu aat Asn 250 ggc gtg cgc gaa Gly Val Arg Giu tgc aac aag cac Cys Asn Lys His ggc atc Gly Ile 260 ctc ttc atc Leu Phe Ilie ctg ttt gct Leu Phe Ala 280 gcc Ala 265 gac gaa gtc atg Asp Glu Val Met gga ttc gga cgc Giy Phe Gly Arg acc gga aaa Thr Gly Lys 275 gac atg atc Asp Met Ile tac gag cat gct Tyr Giu His Ala gac gat ttc cag Asp ASP Phe Gin cca Pro 290 WO 01/00843 WO 0100843PCT/IB00100923 acc ttc 1027 Thr Phe 295 gtg atg 1075 Val Met 310 ggc gga 1123 Gly Gly gca gcg 1171 Ala Ala cga ctt 1219 Arg Leu gaa aac 1267 Glu Asn 375 gtg gag 1315 Val Glu 390 ttc aag 1330 Phe Lys <210> 6 gcc aag ggt gtt Aac Ala Lys Gly Val Asn 300 acc caa tca atc cgc Thr Gin Ser Ile Arg 315 ctc acc tac tcc gga Leu Thr Tyr Ser Gly gca ggt tac gcc cca ctc ggt ggc atc Ala Gly Tyr Ala Pro Leu Gly Gly Ile 305 gat acc ttc gga tca gag gca tac tcc Asp Thr Phe Gly Ser Glu Ala Tyr Ser 320 325 cac cca ctt His Pro Leu gca Al a gta gca ccc gcc aag Val Ala Pro Ala Lys 340 ctg gag Leu Glu 345 ggc gct Giy Ala 360 330 att Ile gaa Glu tac gcg gaa gga Tyr Ala Glu Gly 350 ctg atc gaa cct Leu Ile Glu Pro 335 gag atc att cca cgc gta gct Glu Ile Ile Pro Arg Val Ala 355 cgc ctt cgt gaa cta gcg gaa Arg Leu Arg Glu Leu Ala Glu 370 ggc atc gga ttc ttc tgg gca Gly Ile Giy Phe Phe Trp Aia 365 gta gcg atc gct gac Val Ala Ile Ala Asp 380 ttc aat gca gac gc Phe Asn Ala Asp Ala 395 gtg cgg Val Arg 385 act gcc atg gct gcc Thr Ala Met Ala Ala 400 ggt gct gca gaa Gly Ala Ala Glu 405 gaa cgc ggc Giu Arg Gly 410 <211> 410 <212> PRT <213> Corynebacteriumn glutamicum <400> 6 Leu Ala Leu Lys Gly Tyr Thr Asn Phe Asp Gly 1 5 10 Gly Ser Val Gln Ala Lys Glu Glu Giu Lys Arg 25 Arg Ala His Val Phe His Ser Trp, Ser Ala Gin 40 Lys Val Trp Ala Ala Ala Glu Gly Ser .Thr Leu 55 Asn Ala Phe Ile Asp Met Gly Ser Gln Leu Val 70 Glu Phe Ile Glu Phe Ala Phe Asp Lys Tyr Asp Asp Asn Asp Ile Ser Pro Phe Asp Gly Ser Ala Asn Leu WO 01/00843 PCT/IB00/00923 His Asn Asn Pro Arg Leu Val Glu Ala Ile Gin Arg Gin Ala Ala Arg 90 Leu Thr Asn Ile Asn Pro Ala Phe Gly Asn Asp Val Arg Ser Asp Val 100 105 110 Ala Ala Lys Ile Val Ser Met Ala Arg Gly Glu Phe Ser His Val Phe 115 120 125 Phe Thr Asn Gly Gly Ala Asp Ala Ile Glu His Ser Ile Arg Met Ala 130 135 140 Arg Leu His Thr Gly Arg Asn Lys Ile Leu Ser Ala Tyr Arg Ser Tyr 145 150 155 160 His Gly Ala Thr Gly Ser Ala Met Met Leu Thr Gly Glu His Arg Arg 165 170 175 Leu Gly Asn Pro Thr Thr Asp Pro Asp Ile Tyr His Phe Trp Ala Pro 180 185 190 Phe Leu His His Ser Ser Phe Phe Ala Thr Thr Gin Glu Glu Glu Cys 195 200 205 Glu Arg Ala Leu Lys His Leu Glu Asp Val Ile Ala Phe Glu Gly Ala 210 215 220 Gly Met Ile Ala Ala Ile Val Leu Glu Pro Val Val Gly Ser Ser Gly 225 230 235 240 Ile Ile Leu Pro Pro Ala Gly Tyr Leu Asn Gly Val Arg Glu Leu Cys 245 250 255 Asn Lys His Gly Ile Leu Phe Ile Ala Asp Glu Val Met Val Gly Phe 260 265 270 Gly Arg Thr Gly Lys Leu Phe Ala Tyr Glu His Ala Gly Asp Asp Phe 275 280 285 Gin Pro Asp Met Ile Thr Phe Ala Lys Gly Val Asn Ala Gly Tyr Ala 290 295 300 Pro Leu Gly Gly Ile Val Met Thr Gin Ser Ile Arg Asp Thr Phe Gly 305 310 315 320 Ser Glu Ala Tyr Ser Gly Gly Leu Thr Tyr Ser Gly His Pro Leu Ala 325 330 335 Val Ala Pro Ala Lys Ala Ala Leu Glu Ile Tyr Ala Glu Gly Glu Ile 340 345 350 Ile Pro Arg Val Ala Arg Leu Gly Ala Glu Leu Ile Glu Pro Arg Leu 355 360 365 Arg Glu Leu Ala Glu Glu Asn Val Ala Ile Ala Asp Val Arg Gly Ile 370 375 380 Gly Phe Phe Trp Ala Val Glu Phe Asn Ala Asp Ala Thr Ala Met Ala 385 390 395 400 WO 01/00843 WO 0100843PCTIIBOO/00923 Ala Gly Ala Ala Glu Phe Lys Glu Arg Gly 405 410 <210> 7 <211> 792 <212> DNA <213> Corynebacteriun glut axicun <220> <221> CDS <222> (101)..(769) <223> RXC02390 <400> 7 gctggtggtg ctgacccata cgctggaact ccaactgctg ttgataccgc caagatgttt ggccgcgagg atctcgtagc tcgcttcgag tcataggccg gtg gag tgg acc gct Val Giu Trp Thr Ala ttt ggc acc ctg Phe Gly Thr Leu att Ile ctg ctc aat ttg gtg ggc agt tta tcc Leu Leu Asn Leu Val Gly Ser Leu Ser 15 ccg ggg Pro Giy cct gat acc Pro Asp Thr gcg atc gct Ala Ile Ala ttc ctc ctc cgc Phe Leu Leu Arg gcc acc cgc tcc Ala Thr Arg Ser aga gcg cac Arg Ala His gtg tgg gtg Val Trp Vai ggC gtc gcc ggc Gly Val Ala Gly gtc acc gga ctc Val Thr Gly Leu acg ctg acg gtc gtg gga Thr Leu Thr Val Val Gly att ctc gga atc atc cag Ile Leu Giy Ile Ile Gin gcg gcg ctg ctc Ala Ala Leu Leu act tat ccg tcg Thr Tyr Pro Ser ctc gtc ggc ggc Leu Val Gly Gly tac cta agc ttc Tyr Leu Ser Phe ggg tac aag ttg Gly Tyr Lys Leu ctg Leu cgc tcg gcg tcg Arg Ser Ala Ser gag ctt atc gac Giu Leu Ile Asp gcc cgc Ala Arg 100 cag ttc cgt Gin Phe Arg gca ctg gga Ala Leu Gly 120 aac gcc gat gcc Asn Ala Asp Ala cga Arg 110 cct atc ccg gat Pro Ile Pro Asp gcg gta gaa Ala Val Glu 115 gcc acc aac Ala Thr Asn 451 499 acc cgc act cag Thr Arg Thr Gin gta Val 125 tat cga caa ggt Tyr Arg Gin Gly ctg tca aac cct aaa gtt Leu Ser Asn Pro Lys Val atg tac ttc gcg Met Tyr Phe Ala att ctg gct ccg Ile Leu Ala Pro 547 595 ttg Leu 150 atg cca gcg cac Met Pro Ala His cca Pro 155 tca ccg gtg ctg Ser Pro Val Leu gcg Ala 160 ttc tct atc atc Phe Ser Ile Ile WO 01/00843 WO 0100843PCT11B00100923 gcg Ala gtc Val1 tta gtg Leu Val acg gag Thr Giu 185 gtt Val aaa Lys ttc tct gct gtg tgc ctc att Phe Ser Ala Val Cys Leu Ile 175 180 atg ctg cgt gca ggt ccc tgg Met Leu Arg Ala Gly Pro Trp 195 ctc gtt gtg ggt gtg act ctg Leu Val Val Gly Val Thr Leu 210 ggg taaaggcata aaaaatggct Gly ttt gac ctg ctt gct ggc gtt gtc ttc Phe Asp Leu Leu Ala Gly Val Val Phe 200 205 ctg tat gaa ggc ctg acc ggt tta ctc Leu Tyr Glu Gly Leu Thr Gly Leu Leu 215 220 tcc <210> 8 <211> 223 <212> PRT <213> Corynebacteriun glutamicum <400> 8 Val Glu Trp Thr Ala Phe Gly Thr Leu 1 Ser Arg Leu Thr Tyr Leu Pro Gly Ala 145 Phe Ala Leu Ser Thr Thr Leu Ile Asp Leu 130 Ile Ser Val1 Ser Arg Val Tyr Ser Asp Ala 115 Ala Leu Ile Cys Pro Ala Trp, Pro Phe Ala 100 Val Thr Ala Ile Leu 180 5 Gly His Val Ser Ile Arg Giu Asn Pro Val 165 Ile Asp Ile Leu 55 Leu Tyr Phe Leu Ser 135 Met Ile Ser Thr Ala 40 Thr Giy Lys Arg Gly 120 Asn Pro Leu Thr Phe 25 Giy Val Ile Leu Phe 105 Thr Pro Ala Val Glu 185 Ile 10 Phe Val Val Ile Leu 90 Asn Arg Lys His Gln 170 Leu Leu Asn Leu Al a Gly Gin 75 Arg Ala Thr Val Pro 155 Thr Leu Giy Ala Leu Ser Asp Gin Val 140 Ser Phe Arg Ile Ala Val Ala Ala Val1 125 Met Pro Val Leu Leu Val Ala Gly Ser Arg 110 Tyr Tyr Val1 Thr Val1 Ala Thr Leu Gly Arg Pro Arg Phe Leu Phe 175 Gly Thr Gly Leu Thr Glu Ile Gin Al a Ala 160 Ser Arg Val Arg Lys Ala Met Leu 190 WO 01/00843 PCT/iIBOO/00923 Arg Ala Gly Pro Trp Phe Asp Leu Leu Ala Gly Val Val Phe Leu Val 195 200 205 Val Gly Val Thr Leu Leu Ty'r Glu Gly Leu Thr Gly Leu Leu Gly 210 215 220 <210> 9 <211> 897 <212> DNA <213> Corynebacteriun glutamicun <220> <221> CDS <222> (101)..(874) <223> RXC01796 <400> 9 atgtaactcg atcaggtgga aatgcccgca aaagtggcgg cggtggccga gggatggccg ttggtgcggc atcggtggcc tgctactagt cgggctcttc ttg ctc ctt ggc ggt 115 Leu Leu Leu Gly aac Asn gag Giu Gly ttc Phe atc Ile acc Thr tca Ser cag Gin cac His cc t Pro tct Ser gca Al a tcc Ser gaa Glu ggc Gly gac Asp ttc Phe gag Giu 135 gcc Ala gga Gly ga t Asp gtc Val t ac Tyr tgc Cys caa Gin ggt Gly 120 tac Tyr gag Giu gag Glu gcc Ala aa t Asn acc Thr gg t Gly ga t Asp 105 gca Ala ggc Gly atc Ile tcc Ser aac Asn gaa Giu gag Glu ttc Phe gct Ala gaa Glu cac His gac Asp acc Thr gcc Al a atg Met ccg Pro 75 gct Ala tat Tyr aac Asn c ac His cag Gin gga Gly agt Ser tgg Trp 60 aca thr tct Ser ttt Phe gcc Ala gtc Val 140 gtt Val gcc Ala gat Asp 45 cag Gin ttg Leu gcg Ala gac Asp ccg Pro 125 caa Gin ggC Gly ttt Phe cgc Arg ctt Leu t tc Phe 80 ggg Gly ttc Phe cag Gln gag Giu aac 1 gat Asp gat Asp ct t Leu cca Pro aaa Lys ccg Pro ttC Phe atg Met 9gC Gly 145 gcc caa Gin cac His tac Tyr gc t Ala aac Asn tte Phe ga t Asp tac Tyr 130 aca.

Thr gtc cag Gin caa Gin acc Thr gct Ala acc Thr tgt Cys 100 atg Met gtg Val1 gga Gly atc Gly atc Ile acc Thr tca Ser ggt Gly caa Gin ccg Pro cgt Arg gcg Ala ctg Leu gag tcc aat tac aac gat ccg ggc gct gat WO 01/00843 WO 0100843PCT/IBOO/00923 Ser Asn Tyr Asn ASP Pro Gly Ala A~ 150 155 ttg cag gcc gat tgc tac gca ggc a Leu Gin Ala Asp Cys Tyr Ala Gly I 170 ggc ccg gat ccg cta ctc caa ccc a Giy Pro Asp Pro Leu Leu Gin Pro I 185 1 gct ctc ctt gct gca agc gcc gtg G Ala Leu Leu Ala Ala Ser Ala Val C 200 205 tcc ggt ggc gat gtc aat cct gaa z Ser Gly Gly Asp Val Asn Pro Giu 215 220 cag cgc aaa gac gcg ttc ctc gcc Gin Arg Lys Asp Ala Phe Leu Ala C 230 235 gcc tgc gac ttc ctc ggc cgg ggc 5 Ala Cys Asp Phe Leu Gly Arg Gly 1 250 cttttcgacg tct <210> <211> 258 <212> PRT <213> Corynebacterium giutamicum <400> Leu Leu Leu Gly Gly Asn Pro Ala 1 5 Asp Gin Thr Gin Ile Giu Ser Gly Asp H-is Cys Gin Thr Gly Ala Asp 40 Leu Tyr Tyr Thr Ser Phe Ser Val .55 Pro Ala Gin Ala Gly Ilie Giu Tyr 70 Lys Asn Ser Thr Gin Thr Gly Cys Pro Phe Tyr Cys Pro Ser Asp Gin 100 Phe Asp Gin Met Arg Gin Phe Gly 115 120 Met Tyr Ilie Val Ala 1His Giu Tyr L5p Ltt le ~tc lie .90 jgc ;iy igc er ;gc ly Jtc l 3iu 3iu 25 kla Dsn rhr Gly Asp 105 Ala Gly 14 Ser tgg TrP 175 acc Thr gac Asp tgg Trp tac Tyr t ac Tyr 255 Ile Ser Asn Giu Giu Phe Ala Giu His Asn 160 gc t Ala gaa Giu gac Asp act Thr aac Asn 240 aac Asn Asp Thr Ala Met Pro Ala Tyr Asn His Ala Val aat cac Asn His tct gag Ser Glu aat atc Asn Ile 210 cac ggc His Gly 225 acc ggc Thr Gly gac gct Asp Ala Lys Ilie Giu 165 tcc agc gaa Ser Ser Glu 180 cta gat tcc Leu Asp Ser 195 cag caa cga Gin Gin Arg tca tcg cag Ser Ser Gin cag atg agc Gin Met Ser 245 taaagcattg 643 691 739 787 835 884 Gin Gly Ser Trp, Thr Ser Phe Al a Val Val Ala Asp Gin Leu Ala Asp Pro 125 Gin Leu Gly Asp Thr Thr Ser Leu 110 Leu Asn WO 01/00843 130 Gly Thr Le 145 Ala Val Ly Asn H-is Se Ser Glu Le 19 Asn Ile Gi 210 His Gly Se 225 Thr Giy GI Asp Ala PCTA~BOO/00923 Gly Ile Ser 180 Asp Gin Ser Met Leu Glu 165 Giu Ser Arg Gin Ser 245 Tyr Ala Asp Leu 200 Gly Lys Asp Asn Asp Pro 185 Aia Asp Asp Phe Asp Cys 170 Leu Ala Val Ala Leu 250 140 Gly Ala Gin Ala Pro 220 Leu Arg Ala Gly Pro Val 205 Glu Ala Gly Asp Ile Ile 190 Gly Ser Gly Val1 <210> 11 <211> 771 <212> DNA <213> Corynebacteriun glutamicun <220> <221> CDS <222> (101). .(748) <223> RXCO1207 <400> 11 cttcatgatc tcaccggcag agcgcgtttt gttacagcgc atttttgaac aatccgtaca ccaacttcag gagaaaaaca tgt Cys gca Ala gga Gly gcc Ala agc caa Gin gcc Al a tgc Cys cac His acc gca Ala gtt Val1 aac Asn ga t Asp Ct t gca Ala 15 gtc Vai aac Asn a tg Met gtg ggC Gly ctt Leu gag Giu ccc Pro cac gtaaactgtg actttgaaaa gtg agc aga atc tat Val Ser Arg Ile Tyr 1 cta aag gcg gct gtc Leu Lys Ala Ala Val ccc acg gat acc ctt Pro Thr Asp Thr Leu gca gta gcc aac ctt Ala Val Ala Asn Leu gtt cca gtg ctc gtc Val Pro Val Leu Val tcc tat tct gcg cag 115 163 211 259 307 355 WO 01/00843 Gly Ser Trp Asp Thr Ile Gin Giy Leu Val 75 His Ser Tyr Ser 80 gca aaa gcg ctt gtg gag gcg ttc tgg CC Ala Lys Ala Leu Val Giu Ala Phe Trp Pr 9 gtt ccg cag gca cca agc ctt ccg tgg aa Val Pro Gin Ala Pro Ser Leu Pro Trp As 105 110 acc gta atg ctg cgc atg cca ctg cac cc Thr Val Met Leu Arg Met Pro Leu His Pr 120 125 cgc caa acc gga cca atg gct gtc tcc tc Arg Gin Thr Gly Pro Met Ala Val Ser Se 135 140 act cct cca acc acc gtg ctg gag gct c Thr Pro Pro Thr Thr Val Leu Giu Ala Ax 150 155 gtc gct gtc tac ctc gat ggt ggc gaa t Val Ala Val Tyr Leu Asp Gly Gly Giu C: 170 1 acc atc gtg gat att tca ggc ccc gca cc Thr Ilie Val Asp Ile Ser Gly Pro Ala Pi 185 190 gcc atc agc gca gaa cgc gtt ggc gaa gt Ala Ile Ser Ala Giu Arg Val Gly Giu V~ 200 205 agc ctg cgc taaatgggag tcggtttcgc ggg Ser Leu Arg 215 <210> 12 <211> 216 <212> PRT <213> Corynebacteriun giutanicun <400> 12 Val Ser Arg Ile Tyr Asp Cys Ala Asp G.

1 5 Leu Lys Ala Ala Val Asp Ala Val Lys A.

25 Pro Thr Asp Thr Leu Tyr Gly Leu Gly C: 40 Ala Val Ala Asn Leu Leu Ala Thr Lys H 55 Val Pro Val Leu Val Gly Ser Trp Asp T 70 '5

IC

:0 ggt Gly ctt Leu gtt Val gcc Ala cag Gin 160 gcg Ala aag

LY'S

gga Gly ggc Gly gcc Ala aac Asn 145 cag Gin ctg Leu att Ile ctg Leu gat Asp att Ile 130 atc Ile ctc Leu gCC Ala ttg Leu PCTIIBOOIOO923 Ala Gin atc atc 403 Ile Ile 100 Cgt ggc 451 Arg Gly ttg ctg 499 Leu Leu gga cat 547 Gly His caa aat 595 Gin Asn 165 cct tca 643 Pro Ser 180 gag ggt 691 Giu Gly 11 ctt gga gtg tcg gca gaa Leu Gly Val Ser Ala Giu 210 739 in 10 la y~s is hr Ser Arg Ala Ala Gly Gin Leu Val Val Leu Ala Phe Asn Asn Glu Gly Pro Asp Met Pro Gin Gly Leu Val His WO 01/00843 Ser Tyr Se Giy Leu Se Giy Asp Th 1i Ala Ile Gi 130 Asn Ile Se 145 Gin Leu As Leu Ala Ti, Ile Leu Ar 19 Gly Vai SE 210 PCTIIBOO/00923 Aila Gin Met 120 Thr Pro Val Val1 Ser 200 Arg Leu Ala 105 Leu Giy Thr Tyr Asp 185 Ala Val1 Pro Arg Pro Thr Leu 170 Ile Glu Pro Asn Pro Se r Arg Cys 175 Pro Val <210> 13 <211> 1026 <212> DNA <213> Corynebacterium giutamicum <22 0> <221> CDS <222> (10i)..(1003) <223> RXC00657 <400> 13 gtgcggatcg ggtatccgcg ctacacttag aggtgttaga tgtaacgcag gattcaccaa tcaatgaaag gtcgaccgac att Ilie Cgg Arg gc t Ala cca Pro gca Ala aat Asn acc Thr ctt Leu gta Val acc Thr atg Met ttc Phe gat Asp gcc Ala cct Pro ga t Asp gatcatgagt ttccacgaac atg agc act gaa gac Met Ser Thr Giu Asp 1 tca aaa caa gct gtt Ser Lys Gin Ala Val att cca ctt cgc ttg Ile Pro Leu Arg Leu gca gag gga atg gtt Ala Giu Gly Met Val gaa gcc ctg gaa aag Giu Ala Leu Giu Lys WO 01/00843 att aac Ile Asn atc ggg Ilie Giy atg tct Met Ser gga ctc Gly Leu cac gca His Ala 135 gaa gac Glu Asp 150 gtc tcc Val Ser ggc gcc Gly Ala gca tca Ala Ser gaa cgt Glu Arg 215 gaa aag Giu Lys 230 cca gtt Pro Val gtt ggt Val Gly acc tcc Thr Ser cgc cca 1026 Arg Pro 295 gaa Giu cac His ccc Pro tcc Ser 120 aag Lys agc Ser caa Gin gaa Glu ctt Leu 200 cag Gin tac Tyr cgc Arg tcc Ser cgc Arg 280 cc t Pro gcc Ala acc Thr gat Asp 105 gga Gly tgt Cys aag Lys cag Gin ctc Leu 185 gca Ala caa Gin cca Pro gca Al a cat His 265 gca Ala gag Glu cgt Arg a tc Ile gcc Ala atg Met cca Pro t ac Tyr gca Ala 170 gtt Val ggt Gly acc Thr agt Ser ctt Leu 250 ggt Giy c tg Leu aag Lys gac Asp 75 gc t Aila aca Thr gte Val1 gtc Val1 ggc Gly 155 acc Thr gca Aila ct t Leu gac

ASP

gta Vali 235 gca Ala cgt Arg ctg Leu at t Ile atc Ile gaa Giu atg Met a tg Met gtt Val 140 cca Pro gaa Giu gtt Vai gca Ala atg Met 220 acc Thr gaa Glu ggc Gly caa Gin aag Lys 300 gcc Al a ggc Gly atc Ile Giy 125 gtt Val gtc Val1 tac Tyr cac His gc t Al a 205 ctg Leu gtc Val1 gca Aila gga Gly tcc Se r 285 aag Lys cat His agt Ser gtc Vai 110 tcc Ser gtc Val1 gtc Val gca Ala acc Thr 190 gct Ala atc Ilie aag Lys tc t Ser ttt Phe 270 gca gaa Giu ctg Leu gga Giy 115 gtg Val1 gca Ala 9gc Gly gaa Glu cag Gin 195 gat Asp cca Pro cgt Arg c tc Leu ctt Leu 275 atg PCTIIBOO/00923 atc aag 355 Ile Lys ttg gaa 403 Leu Giu 100 ctc ggc 451 Leu Gly gtc agc 499 Val Ser gtc aac 547 Val Asn tcc gaa -595 Ser Giu 165 gct cgt 643 Aia Arg 180 gta cag 691 Val Gin gaa gtg 739 Giu Val ctg gtg 787 Leu Val gac cgc 835 Asp Arg 245 cta gtc 883 Leu Val 260 ggc tcc 931 Gly Ser gtg gtt 979 Aia Pro Cys Pro Met Met Vai Val 290 tagtttcttt taagtttcga tgc WO 01/00843 WO 0100843PCT/EBOO/00923 <210> 14 <211> 301 <212> PRT <213> Corynebacterium giutarnicum <400> 14 Met Ser Thr Giu Asp Ile Val Val Val Ala Val Asp Gly Ser Asp Ala 1 5 10 Ser Lys Gin Ala Val Arg Trp Ala Ala Asn Thr Ala Asn Lys Arg Gly 25 Ile Pro Leu Arg Leu Ala Ser Ser Tyr Thr Met Pro Gin Phe Leu Tyr 40 Ala Giu Gly Met Val Pro Pro Gin Glu Leu Phe Asp Asp Leu Gin Ala 55 Giu Ala Leu Glu Lys Ile Asn Giu Ala Arg Asp Ile Ala H-is Giu Val 70 75 Ala Pro Giu Ile Lys Ile Gly H-is Thr Ile Ala Giu Gly Ser Pro Ile 90 Asp Met Leu Leu Glu Met Ser Pro Asp Ala Thr Met Ile Val Met Gly 100 105 110 Ser Arg Gly Leu Gly Gly Leu Ser Gly Met Val Met Gly Ser Val Ser 115 120 125 Gly Ala Val Val Ser Hius Ala Lys Cys Pro Val Val Val Val Arg Giu 130 135 140 Asp Ser Ala Val Asn Glu Asp Ser Lys Tyr Gly Pro Val Val Val Gly 145 150 155 160 Val Asp Gly Ser Giu Val Ser Gin Gin Ala Thr Giu Tyr Ala Phe Ala 165 170 175 Giu Ala Giu Ala Arg Gly Ala Giu Leu Val Ala Val His Thr Trp Met 180 185 190 Asp Met Gin Val Gin Ala Ser Leu Ala Gly Leu Ala Ala Ala Gin Gin 195 200 205 Gin Trp, Asp Giu Val Giu Arg Gin Gin Thr Asp Met Leu Ile Giu Arg 210 215 220 Leu Ala Pro Leu Val Giu Lys Tyr Pro Ser Val Thr Val Lys Lys Ile 225 230 235 240 Ile Thr Arg Asp Arg Pro Val Arg Ala Leu Ala Giu Ala Ser Giu Asn 245 250 255 Ala Gin Leu Leu Val Val Gly Ser His Gly Arg Gly Gly Phe Lys Gly 260 265 270 Met Leu Leu Gly Ser Thr Ser Arg Ala Leu Leu Gin Ser Ala Pro Cys 275 280 285 WO 01/00843 WO 0100843PCTIBOO/00923 Pro Met Met Val Val Arg Pro Pro Giu Lys Ile Lys Lys 290 295 300 <210> <211> <212> <213> <220> <221> <222> <223> 1059

DNA

Corynebacterium

CDS

(101)..(1036) RXC 00552 gi utamicun <400> ccgccaacaa ggcagcaaag ctcgatccaa ttgacgcctt gcgttatgag taaaagcctc gtttttaagg tagccacaca tcgcactaga ctgaagaact gtg gct acc tca Val Ala Thr Ser att ctt ctt tat Ilie Leu Leu Tyr tac Tyr gca ttc acc ccg Ala Phe Thr Pro tct gac cct aaa Ser Asp Pro Lys gcg gtt Ala Val cag ctg tgg Gin Leu Trp atc ctg atc Ile Leu Ile cag Gin cgt gag ctc tgc Arg Glu Leu Cys tca ctg aat ctt cgt ggc cgc Ser Leu Asn Leu Arg Gly Arg tcc act cac ggc Ser Thr His Gly aat gga acc gtg Asn Gly Thr Val gga gat att Gly Asp Ile gat gat Asp Asp tgc aag gcg tac Cys Lys Ala Tyr att Ile 60 aaa aag acc cgc Lys Lys Thr Arg gag Giu tac cca ggt ttc Tyr Pro Gly Phe cgc atg cag ttt Arg Met Gin Phe tgg tcc gag ggt Trp, Ser Giu Gly gct gag gat ttc Ala Glu Asp Phe aag ctc agt gtc Lys Leu Ser Val aaa Lys gtc cgc gat gag Val Arg Asp Glu gtt gcc ttc ggc Val Ala Phe Gly gct cca Ala Pro 100 gat gag ctc Asp Glu Leu ctg aaa cca Leu Lys Pro 120 gtg gat gaa aac Val Asp Glu Asn gtc gtc ggt ggc Val Val Gly Gly ggc gtt cac Gly Val His 115 ggc gat gaa Gly Asp Glu cag cag gtc aat Gln Gin Val Asn ctt gtg gaa gcc Leu Val Giu Ala gtt gtg Val Val 135 ttc ttt gac ggc Phe Phe Asp Gly cgc Arg 140 aac gca atg gaa Asn Ala Met Giu gcc Ala 145 cag atc ggc aag Gin Ile Gly Lys aag gac gct gtt Lys Asp Ala Vai cct gac gta gaa Pro ASP Val Giu act cat gat ttc Thr His Asp Phe WO 01/00843 WO 0100843PCTlIBOOIOO923 gca gaa att gag tct gga aaa tac gac gat Ala Glu Ile Giu Ser Gly Lys Tyr Asp Asp 170 175 gtc acc tac tgc acc ggc gga att cgt tgt Val Thr Tyr Cys Thr Gly Gly Ile Arg Cys 185 190 atg atc aac cgt ggt ttc aaa gag gtc tac Met Ilie Asn Arg Gly Phe Lys Giu Val Tyr 200 205 gtt cgc tac ggc gag cag ttt ggc aac aag Val Arg Tyr Gly Glu Gin Phe Gly Asn Lys 215 220 ctc tac gtt ttc gat aag cgc atg cat atg Leu Tyr Val Phe Asp Lys Arg Met His Met 230 235 aaa gag gtc gga cac tgc atc cat tgc gat Lys Giu Val Giy His Cys Ile His Cys Asp 250 255 gag cac tgc ctc aac gaa gat gat tgc cgc Giu His Cys Leu Asn Giu Asp Asp Cys Arg 265 270 cct gat tgc ttc gcc aat gtt gag acc cgt Pro Asp Cys Phe Ala Asn Val Giu Thr Arg 280 285 tgt gca gca att gct gcg gat ttc gct gag 1027 Cys Ala Ala Ile Ala Ala Asp Phe Ala Glu 295 300 gtt act tct taaaaagggt atggtggctg ggt 1059 Val Thr Ser 310 <210> 16 <211> 312 <212> PRT <213> Corynebacterium giutamicun <400> 16 Val Ala Thr Ser Lys Ilie Leu Leu Tyr Tyr 1 5 10 Asp Pro Lys Ala Val Gin Leu Trp Gin Arg 25 Asn Leu Arg Gly Arg Ilie Leu Ilie Ser Thr 40 Val Gly Gly Asp Ile Asp Asp Cys Lys Ala 55 ctc aaa gac Leu Lys Asp gag atc ctg Giu Ile Leu caa atc gat Gin Ile Asp 210 ggc ctg tgg Giy Leu Trp 225 gaa ttc ggc Giu Phe Gly 240 act ccc acc Thr Pro Thr gag ctc gtg Giu Leu Val cat tgc aag His Cys Lys 290 caa gga att Gin Gly Ile aag cct LYS Pro 180 agt tca Ser Ser 195 ggc ggc Gly Gly gaa ggc Giu Gly gag gat Glu Asp aac aaa Asn Lys 260 ttg atg Leu Met 275 cgc gaa Arg Giu gat ccg Asp Pro atci 643 691 739 787 835 883 931 979 Ala Phe Thr Pro Leu Ser Giu Leu Cys Giu Ser Leu His Gly Ile Asn Gly Thr Tyr Ile Lys Lys Thr Arg WO 01/00843 PCT/IBOO/00923 Glu Tyr Pro Gly Phe Asn Arg Met Gin Phe Lys Trp Ser Giu Gly Gly 70 75 Ala Giu Ala Phe C Gly Gly Ala Arg C 130 Ala Gin 145 Thr His 2 Lys Asp I Ile Leu Ile Asp 210 Leu Trp 225 Phe Gly Pro Thr Leu Val Cys Lys 290 Gly Ile 305 <210> 17 ~sp ly 'ly ;ly Ile ksp -ys Ser 31y Glu lu Asn Leu 275 Arg Asp Phe Ala 100 Val Asp Gly Phe Pro 180 Ser Gly Gly Asp Lys 260 Met Glu Pro Pro Pro His Glu Lys Ile 165 Va1 Leu Ile Ser Tyr 245 Phe Cys Arg Leu Lys Leu Asp Glu Leu Lys Val Val 135 Phe Lys 150 Ala Glu Vai Thr Met Ile Val Arg 215 Leu Tyr 230 Lys Glu Glu His Pro Asp Cys Ala 295 Val Thr 310 Ser Leu Pro 120 Phe Asp Ile Tyr Asn 200 Tyr Va1 Va1 Cys Cys 280 Ala Ser Val Lys 105 Gin Phe Ala Glu Cys 185 Arg Gly Phe Gly Leu 265 Phe Ile Lys 90 Val Gin Asp Val Ser 170 Thr Gly Glu Asp His 250 Asn Ala Ala Val Asp Val Gly Val 155 Gly Gly Phe Gin Lys 235 Cys Glu Asn Ala Arg Glu Asn Arg 140 Pro Lys Gly Lys Phe '220 Arg Ile Asp Va1 Asp 300 Asp Asn Glu 125 Asn Asp Tyr Ile Glu 205 Gly Met His Asp Glu 285 Phe Glu Gly 110 Leu Ala Val Asp Arg 190 Val Asn His Cys Cys 270 Thr Ala Ile Va1 Va1 Met Glu Asp 175 Cys Tyr Lys Met Asp 255 Arg Arg Glu Va1 Val Glu Glu Thr 160 Leu Glu Gin Gly Glu 240 Thr Glu His Gin <211> 1578 <212> DNA <213> Corynebacterium giutaxicum <220> <221> CDS <222> (10)..(1555) <223> RXN00351 <400> 17 gaaggctgct gctaagaaaa cgaccaagaa gaccactaag aaaactacta aaaagaccac WO 01/00843 WO 0100843PCTIiBOO/00923 cgcaaagaag accacaaaga agtcttaagc cggatcttat atg gat gat tcc aat 115 Met Asp Asp Ser Asn 1 agc ttt gta gtt gtt gCt aac cgt ctg cca gtg gat atg act gtc cac 163 Ser Phe Vai Val Val Ala Asn Arg Leu Pro Val Asp Met Thr Val His 15 cca gat ggt agc tat agc atc tcc ccc agc ccc ggt ggc ctt gtc acg 211 Pro Asp Giy Ser Tyr Ser Ile Ser Pro Ser Pro Gly Giy Leu Vai Thr 30 ggg ctt tcc ccc gtt ctg gaa caa cat cgt gga tgt tgg gtc gga tgg 259 Gly Leu Ser Pro Val Leu Giu Gin His Arg Gly Cys Trp Val Giy Trp 45 cct gga act gta gat gtt gca ccc gaa cca ttt cga aca gat acg ggt 307 Pro Gly Thr Vai Asp Val Ala Pro Glu Pro Phe Arg Thr Asp Thr Gly 60 gtt ttg ctg cac cct gtt gtc ctc act gca agt gac tat gaa ggc ttc 355 Vai Leu Leu His Pro Val Val Leu Thr Ala Ser Asp Tyr Glu Gly Phe 75 80 tac gag ggc ttt tca aac gca acg ctg tgg cct ctt ttc cac gat ctg 403 Tyr Glu Gly Phe Ser Asn Ala Thr Leu Trp Pro Leu Phe His Asp Leu 95 100 att gtt act ccg gtg tac aac acc gat tgg tgg cat gcg ttt cgg gag 451 Ilie Val Thr Pro Val Tyr Asn Thr Asp Trp Trp His Ala Phe Arg Glu 105 110 115 gta aac ctc aag ttc gct gaa gcc gtg agc caa gtg gcg gca cac ggt 499 Val Asn Leu Lys Phe Ala Giu Ala Vai Ser Gin Val Ala Ala His Giy 120 125 130 gcc act gtg tgg gtg cag gac tat cag ctg ttg ctg gtt cct ggc att 547 Ala Thr Val Trp, Val Gin Asp Tyr Gin Leu Leu Leu Vai Pro Gly Ile 135 140 145 ttg cgc cag atg cgc cct gat ttg aag atc ggt ttc ttc ctc cac att 595 Leu Arg Gin Met Arg Pro Asp Leu Lys Ile Gly Phe Phe Leu His Ile 150 155 160 165 ccc ttc cct tcc cct gat ctg ttc cgt cag ctg ccg tgg cgt gaa gag 643 Pro Phe Pro Ser Pro Asp Leu Phe Arg Gin Leu Pro Trp, Arg Glu Giu 170 175 180 att gtt cga ggc atg ctg ggc gca gat ttg gtg gga ttc cat ttg gtt 691 Ilie Val Arg Gly Met Leu Gly Ala Asp Leu Val Gly Phe His Leu Val 185 190 195 caa aac gca gaa aac ttc ctt gcg tta acc cag cag gtt gcc ggc act 739 Gin Asn Ala Giu Asn Phe Leu Ala Leu Thr Gin Gin Val Ala Gly Thr 200 205 210 gcc ggg tct cat gtg ggt cag ccg gac acc ttg cag gtc agt ggt gaa 787 Ala Giy Ser His Val Giy Gin Pro Asp Thr Leu Gin Vai Ser Giy Giu 215 220 225 WO 01/00843 WO 0100843PCT/IBOO/00923 gca Ala 230 cga Arg ttg gtg cgt gag Leu Val Arg Glu att Ile 235 gcg Ala ggc gct cat gtt Gly Ala His Val gaa Giu 240 att Ile acc gct gac gga Thr Ala Asp Gly gtt agc gtc Val Ser Val ggg Gly 250 aaa Lys ttc ccg atc Phe Pro Ile gat gtt gaa Asp Val Glu atg ttt Met Phe 260 ctc gac Leu Asp ggg gag gcg Gly Glu Ala gag ccg gaa Giu Pro Glu 280 agc gcc gtt Ser Ala Val ctt tta aaa Leu Leu Lys acg Thr 275 gta ttc ctg Val Phe Leu gac cga ctg Asp Arg Leu ggc att 1027 Gly Ilie 295 gcg ttg 1075 Ala Leu 310 cgt gag 1123 Arg Glu gcc gtc 1171 Ala Val gtg cat 1219 Val His tat acc 1267 Tyr Thr 375 aac ttg 1315 Asn Leu 390 gCt ttg 1363 Ala Leu gcg tat 1411 Ala Tyr ttg cag cgc ctg ctt Leu Gin Arg Leu Leu 300 gag gcc gac aaa gct Glu Ala Asp Lys Ala 315 cgc att gat cac tat Arg Ilie Asp His Tyr ttt gag gaa ctg Phe Glu Giu Leu 305 gtg ttg ctg cag Val Leu Leu Gin 320 gtc Val1 gac tac acc aag Asp Tyr Thr Lys 290 ctg gaa tcc ggc Leu Glu Ser Gly gcg acg cct tcg Ala Thr Pro Ser 325 cag gtc gag gaa Gln Val Glu Giu 340 979 cgt gtg tcg Arg Val Ser cgt Arg ggc cgt Gly Arg 345 tat cta Tyr Leu 360 330 atc Ile c ac His aat ggt cgt ttc Asn Gly Arg Phe 350 agg tca ttg agc Arg Ser Leu Ser 335 ggt cgc atg ggg cgt ccc gtg Gly Arg Met Gly Arg Pro Val 355 aaa aat gat ctc cag gtg ctg Lys Asn Asp Leu Gin Val Leu 370 365 gca gcc gat gtc at~g Ala Ala Asp Val Met 380 gtg gct aaa gaa ttc Val Ala Lys Glu Phe 395 gtg ctg tcc gaa ttt Val Leu Ser Glu Phe 410 tta tgc aac cca ttt Leu Cys Asn Pro Phe 425 ctg Leu gtg Val gtt acg cct ttt aaa gac ggt atg Val Thr Pro Phe Lys Asp Gly Met 385 gcc aac cac cgc gac ggc act ggt Ala Asn His Arg Asp Gly Thr Gly 400 gcc ggc gcg Ala Gly Ala 415 gat gtg gaa Asp Val Glu 430 gcc act gag ctg acc ggt Ala Thr Glu Leu Thr Gly .420 tcc atc aaa cgg caa atg Ser Ilie Lys Arg Gin Met 435 WO 01/00843 WO 0100843PCT/IBOO/00923 gtg gca gct gtc cat gat ttg aag cac aat 1459 Val Ala Ala Val His Asp Leu Lys His Asn 440 445 cga atg aaa acg aac agc gag cag gtc tat 1507 Arg Met Lys Thr Asn Ser Glu Gin Val Tyr 455 460 tgg gct aat agt ttc ctg gat tgt ttg gca 1555 Trp Ala Asn Ser Phe Leu Asp Cys Leu Ala 470 475 tgaaccgcgc acgaatcgcg acc 1578 <210> 18 211> 485 <212> PRT <213> Corynebacterium giutarnicum <400> 18 Met Asp Asp Ser Asn Ser Phe Val Val 1 Asp Gly Cys Arg Asp Leu His Val Leu 145 Phe Pro Met Gly Trp Thr Tyr Phe Ala Ala 130 Val1 Phe Trp Val Val1 Gly Thr Gly Asp 100 Arg His Gly His Glu 180 5 His Thr Trp Gly Phe Leu Glu Gly Ile Ile 165 Glu Val Tyr Val Asp Pro Ser Val Phe Val1 Arg Pro 170 Met ccg Pro acc Thr cag Gin 480 Ala Ser Leu Val1 Val 75 Asn Tyr Ala Gin Pro 155 Asp Leu gaa tct gcg gca Glu Ser Ala Ala 450 cac gac gtc aac His Asp Val Asn 465 tcg gga gaa aac Ser Gly Glu Asn Asn Ile Glu Ala Val Ala Asn Glu

ASP

140 Asp Leu Gly Arg Ser Gin Pro Leu Thr Thr Ala 125 Tyr Leu Phe Ala Leu Pro His Glu Thr Leu Asp 110 Val1 Gin Lys Arg Asp 190 acg Thr gtg Val1 tca Ser 485 Val Pro Gly Phe Ser Pro Trp, Gin Leu Gly 160 Leu Val1 WO 01/00843 WO 0100843PCTIB00100923 Gly Gin Gin 225 Thr Asp Leu Leu Leu 305 Val Ser Met Asp Phe 385 Arg Thr Ile Giu His 465 Ser Phe His Leu Val Gin Asn Ala Glu Asn Phe Leu Ala Leu Thr Gin 195 200 205 Vai 210 Val Ala Val Lys Asp 290 Leu Al a Gin Gly Leu 370 Lys Asp Glu Lys Ser 450 Asp Gly Al a Ser Asp Glu Thr 275 Tyr Glu Thr Val1 Arg 355 Gin Asp Giy Leu Arg 435 Al a Val Glu Gly Gly Giy Met 260 Leu Thr Se r Pro Giu 340 Pro Val1 Gly Thr Thr 420 Gin Ala Asn Asn Thr Giu Arg 245 Phe Asp Lys Gly Ser 325 Giu Val Leu Met Gly 405 Gly Met Thr Val1 Ser 485 Ala Ala 230 Arg Gly Giu Gly Ala 310 Arg Ala Val Tyr Asn 390 Ala Ala Val1 Arg Trp 470 Gly 215 Leu Val1 Glu Pro Ile 295 Leu Giu Val His Thr 375 Leu Leu Tyr Ala Met 455 Al a Ser Val1 Ser Ala Giu 280 Leu Glu Arg Gly Tyr 360 Ala Val Val Leu Ala 440 Lys Asn His Arg Val Ser 265 Thr Gin Ala Ile Arg 345 Leu Ala Ala Leu Cys 425 Val Thr Ser Val1 Glu Gly 250 Lys Val1 Arg Asp Asp 330 Ile His Asp Lys Ser 410 Asn His Asn Phe Gly I le 235 Ala Ser Phe Leu Lys 315 His Asn Arg Val Giu 395 Glu Pro Asp Ser Leu 475 Gin 220 Gly Phe Ala Leu Leu 300 Ala Tyr Gly Ser Met 380 Phe Phe Phe Leu Giu 460 Asp Pro Ala Pro Val Gly 285 Al a Val Arg Arg Leu 365 Leu Val1 Ala Asp Lys 445 Gin Cys Asp His Ile Leu 270 Val Phe Leu Val Phe 350 Ser Val1 Al a Gly Val1 430 His Val1 Leu Thr Val1 Ser 255 Asp Asp Glu Leu Se r 335 Gly Lys Thr Asn Ala 415 Glu Asn Tyr Ala Leu Giu 240 Ile Leu Arg Glu Gin 320 Arg Arg Asn Pro His 400 Ala Ser Pro Thr Gin 480 <210> 19 <211> 1546 <212> DNA WO 01/00843 WO 0100843PCT/iBOO/00923 <213> Corynebacteriun giutamicuni <2 <221> CDS <222> (101)..(1546) <223> FRXA00351 <400> 19 gaaggctgct gctaagaaaa cgaccaagaa gaccactaag aaaactacta aaaagaccac cgcaaagaag accacaaaga agtcttaagc cggatcttat atg gat g&t tcc aat Met ASP ASP Ser Asn agc ttt gta gtt Ser Phe Val Val gct aac cgt ctg Ala Asn Arg Leu gtg gat acg act Val Asp Met Thr gtc cac Val His cca gat ggt Pro Asp Gly ggg ctt tcc Gly Leu Ser tat agc atc tcc ccc agc ccc ggt ggc Tyr Ser Ile Ser Pro Ser Pro Gly Gly ctt gtc acg Leu Val Thr gtc gga tgg Val Gly Trp ccc gtt ctg gaa Pro Val Leu Glu cat cgt gga tgt His Arg Gly Cys t gg Trp cct gga Pro Gly act gta gat gtt Thr Val Asp Val ccc gaa cca ttt Pro Giu Pro Phe aca gat acg ggt Thr Asp Thr Gly ttg ctg cac cct Leu Leu His Pro gtc ctc act gca Val Leu Thr Ala ag t Ser gac tat gaa ggc Asp Tyr Giu Gly tac gag ggc ttt Tyr Giu Gly Phe aac gca acg ctg Asn Ala Thr Leu tgg Trp cct ctt ttc cac Pro Leu Phe His gat ctg Asp Leu 100 att gtt act Ile Val Thr gta aac ctc Val Asn Leu 120 gtg tac aac acc Val Tyr Asn Thr tgg tgg cat gcg ttt cgg gag Trp Trp His Ala Phe Arg Giu 115 aag ttc gct gaa Lys Phe Ala Giu gtg agc caa gtg Val Ser Gin Val gca cac ggt Ala His Gly gcc act Ala Thr 135 gtg tgg gtg cag Val Trp, Val Gin tat cag ctg ttg ctg gtt cct ggc att Tyr Gin Leu Leu Leu Val Pro Gly Ile 145 t tg Leu 150 cgc cag atg cgc Arg Gin Met Arg cc t Pro 155 gat ttg aag atc Asp Leu Lys Ile ttc ttc ctc cac Phe Phe Leu His ccc ttc cct tcc Pro Phe Pro Ser gat ctg ttc cgt Asp Leu Phe Arg cag Gin 175 ctg ccg tgg cgt Leu Pro Trp Arg gaa gag Giu Giu 180 att gtt cga Ile Val Arg ggc Gly 185 atg ctg ggc gca Met Leu Gly Ala ga t Asp 190 ttg gtg gga ttc Leu Val Gly Phe cat ttg gtt His Leu Val 195 WO 01/00843 WO 0100843PCTJIBOO/00923 caa aac gca Gin Asn Ala 200 gcc ggg tct Ala Giy Ser 215 gca ttg gtg Ala Leu Val 230 cga gtt agc Arg Val Ser ggg gag gcg Gly Giu Ala gag ccg gaa Glu Pro Glu 280 ggc att ttg 1027 Gly Ilie Leu 295 gcg ttg gag 1075 Ala Leu Glu 310 cgt gag cgc 1123 Arg Glu Arg gcc gtc ggc 1171 Ala Val Gly gtg cat tat 1219 Val Hius Tyr 360 tat acc gca 1267 Tyr Thr Ala 375 aac ttg gtg 1315 gaa aac ttc ctt gcg tta acc Glu Asn Phe Leu Ala Leu Thr cag cag gtt gcc ggc act Gln Gin Val Ala Gly Thr 739 205 210 cat gtg Hius Val cgt gag Arg Giu gtc ggg Val Gly 250 tcg aaa Ser Lys 265 acc gta Thr Val cag cgc Gin Arg gcc gac Ala Asp att gat Ilie Asp 330 cgt atc Arg Ile 345 cta cac Leu His gcc gat Ala Asp gct aaa ggt G ly att Ile 235 gcg Ala agc Ser t tc Phe ctg Leu aaa Lys 315 cac His aat Asn agg Arg gtc Val gaa cag Gin 220 ggc Gly ttc Phe gcc Ala ctg Leu ctt Leu 300 gc t Al a tat Tyr ggt Gly tca Ser a tg Met 380 ttc ccg Pro gc t Ala ccg Pro gt t Val ggc Gly 285 gcg Ala gtg Val1 cgt Arg cgt Arg t tg Leu 365 ctg Leu gtg gac Asp cat His atc Ile ctt Leu 270 gt t Val1 ttt Phe t tg Leu gtg Val1 ttc Phe 350 agc Ser gtt Val1 g cc acc Thr gtt Val1 tcg Ser 255 gat Asp gaq Asp gag Giu ctg Leu tcg Ser 335 ggt G ly aaa Lys acg Thr aac t tg Leu gaa Glu 240 att I le ctt Leu cga Arg gaa G lu cag Gin 320 cgt Arg cgc Arg aa t Asn cct Pro cac cag Gin 225 acc Thr ga t Asp tta Leu ctg Leu ctg Leu 305 gtc Val tcg Ser atg Met ga t Asp ttt Phe 385 cgc gtc Val1 gc t Ala gtt Val1 aaa Lys gac Asp 290 ctg Leu gcg Ala cag Gln ggg Gly c tc Leu 370 aaa Lys gac agt Ser gac Asp gaa Glu acg Thr 275 tac Tyr gaa Glu acg Thr gtc Val cgt Arg 355 cag Gln gac Asp 99C ggt gaa Gly Glu gga agg Gly Arg 245 atg ttt Met Phe 260 ctc gac Leu Asp acc aag Thr Lys tcc ggc Ser Gly cct tcg Pro Ser 325 gag gaa Glu Giu 340 ccc gtg Pro Val gtg ctg Val Leu ggt atg Gly Met act ggt Asn Leu Val Ala Lys Glu Phe Val Ala Asn His Arg Asp Gly Thr Gly 405 wo 01/00843 WO 0100843PCTLBOOIOO923 gct ttg gtg ctg tcc gaa ttt gcc ggc gcg gcc act gag ctg acc ggt 1363 Ala Leu Val Leu Ser Giu Phe Ala Gly Ala Ala Thr Glu Leu Thr Gly 410 415 420 gcg tat tta. tgc aac cca ttt gat gtg gaa. Ccc atc aaa cgg caa atg 1411 Ala Tyr Leu Cys Asn Pro Phe Asp Val Giu Ser Ile Lys Arg Gin Met 425 430 435 gtg gca gct gtc cat gat ttg aag cac aat ccg gaa tct gcg gca acg 1459 Val Ala Ala Val His Asp Leu Lys His Asn Pro Giu Ser Ala Ala Thr 440 445 450 cga atg aaa acg aac agc gag cag gtc tat acc cac gac gtc aac gtg 1507 Arg Met Lys Thr Asn Ser Glu Gin Val Tyr Thr His Asp Val Asn Val 455 460 465 tgg gct aat agt ttc ctg gat tgt ttg gca cag tcg gga 1546 Trp Ala Asn Ser Phe Leu Asp Cys Leu Ala Gin Ser Gly 470 475 480 <210> <211> 482 <212> PRT <213> Corynebacterium glutamicum <400> Met Asp Asp Ser Asn Ser Phe Val Val Val Ala Asn Arg Leu Pro Val 1 5 10 Asp Met Thr Val His Pro Asp Gly Ser Tyr Ser Ile Ser Pro Ser Pro 25 Gly Gly Leu Val Thr Gly Leu Ser Pro Val Leu Giu Gin His Arg Gly 40 Cys Trp, Val Gly Trp Pro Gly Thr Val Asp Val Ala Pro Glu Pro Phe 55 Arg Thr Asp Thr Gly Val Leu Leu His Pro Val Val Leu Thr Ala Ser 70 75 Asp Tyr Giu Gly Phe Tyr Giu Gly Phe Ser Asn Ala Thr Leu Trp Pro 90 Leu Phe His Asp Leu Ile Val Thr Pro Val Tyr Asn Thr Asp Trp Trp 100 105 110 His Ala Phe Arg Glu Val Asn Leu Lys Phe Ala Giu Ala Val Ser Gin 115 120 125 Val Ala Ala His Gly Ala Thr Val Trp Val Gin Asp Tyr Gin Leu Leu 130 135 140 Leu Val Pro Gly Ile Leu Arg Gin Met Arg Pro Asp Leu Lys Ile Gly 145 150 155 160 WO 01/00843 WO 0100843PCT/IBOO/00923 Phe Pro Gly Gin Gin 225 Thr Asp Leu Leu Leu 305 Val Ser Met Asp Phe 385 Arg Thr Ile Giu His 465 Phe Trp, Phe Val 210 Vali Ala Vai Lys Asp 290 Leu Aia Gin Giy Leu 370 Lys Asp Giu Lys Ser 450 Asp Leu Arg His 195 Aia Ser Asp Giu Thr 275 Tyr Giu Thr Vai Arg 355 Gin Asp Giy Leu Arg 435 Ala Val His Ile 165 Glu Giu 180 Leu Vai Gly Thr Gly Giu Gly Arg 245 Met Phe 260 Lieu Asp Thr Lys Ser Giy Pro Ser 325 Glu Giu 340 Pro Val Vai Leu Gly Met Thr Giy 405 Thr Gly 420 Gin Met Ala Thr Asn Val Pro Ile Gin Ala Ala 230 Arg Gly Glu Gly Al a 310 Arg Ala Val1 Tyr Asn 390 Ala Ala Val1 Arg Trp 470 Phe Val Asn Giy 215 Leu Val1 Giu Pro Ile 295 Leu Giu Val His Thr 375 Leu Leu Tyr Al a Met 455 Ala Pro Arg Ala 200 Ser Val Ser Ala Giu 280 Leu Giu Arg Gly Tyr 360 Ala Val Val Leu Ala 440 Lys Asn Ser Gly 185 Glu His Arg Val Ser 265 Thr Gin Aia Ile Arg 345 Leu Ala Ala Leu Cys 425 Val1 Thr Ser Pro 170 me t Asn Val1 Giu Gly 250 Lys Val1 Arg Asp Asp 330 Ile His Asp Lys Ser 410 Asn His Asn Phe Asp Leu Phe G ly Ile 235 Ala S er Phe Leu Lys 315 His Asn Arg Val Glu 395 Giu Pro Asp Ser Leu 475 Leu Phe Gly Ala Leu Ala 205 Gin Pro 220 Gly Ala Phe Pro Ala Val Leu Gly 285 Leu Ala 300 Ala Val Tyr Arg Gly Arg Ser Leu 365 Met Leu 380 Phe Val Phe Ala Phe Asp Leu Lys 445 Giu Gin 460 Asp Cys Arg Asp 190 Leu Asp His Ile Leu 270 Val Phe Leu Val1 Phe 350 Ser Val Ala Gly Val 430 His Val Leu Gin 175 Leu Thr Thr Val1 Ser 255 Asp Asp Giu Leu Ser 335 Gly Lys Thr Asn Ala 415 Giu Asn Tyr Ala Leu Val1 Gin Leu Giu 240 Ile Leu Arg Giu Gin 320 Arg Arg Asn Pro His 400 Ala Ser Pro Thr Gin 480 WO 01/00843 WO 0100843PCT/IBOO/00923 Ser Gly <210> 21 <211> 779 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> <223> RXA00873 <400> 21 act gcc caa Thr Ala Gin 1 gaa atg gtc Giu Met Val tcc gaa cct Ser Giu Pro cca ctg. ctt Pro Leu Leu tgg ggt att ttc ctg cgt Trp Gly Ile Phe Leu Arg 5 cat gat gag ctc His Asp Glu Leu acc ctt Thr Leu tcc Ser gat gag gaa cgc Asp Giu Giu Arg agc Ser 25 tac atg tac tcc Tyr Met Tyr Ser caa ttc gcc Gin Phe Ala cgc ctt tcc Arg Leu Ser cgc atg cgc gcc Arg Met Arg Ala gta gga atc cgc Val Gly Ile Arg gaa ggc gac cgc aac cag ctg gaa Glu Gly Asp Arg Asn Gin Leu Giu ctt cac ggt ttg Leu His Gly Leu ctg tct cta cct Leu Ser Leu Pro ggc Gly tca ccc gtg ttg Ser Pro Val Leu tac ggt gat gaa Tyr Gly Asp Glu ggc atg ggc gac Gly Met Gly Asp atc tgg ctc cac Ile Trp LeU His cgc gac gga gtg Arg Asp Gly Val cgc acc Arg Thr ccc atg cag Pro Met Gin cct gaa cgc Pro Giu Arg 115 tcc aac gac cgc Ser Asn Asp Arg ggt ggt ttc tcc Gly Gly Phe Ser aaa gct gat Lys Ala Asp 110 tac ggc tac Tyr Gly Tyr ctg tac ctt cca Leu Tyr Leu Pro atc caa aat gat Ile Gin Asn Asp caa Gin 125 gcc caa Ala Gin 130 gta aac gtg gaa Val Asn Val Glu caa ctc aac cgc Gin Leu Asn Arg aac tcc ctg ctg Asn Ser Leu Leu cgc Arg 145 tgg ctc cga aac Trp, Leu Arg Asn atc ctt atc cgc Ile Leu Ile Arg cag tac cgc gca Gin Tyr Arg Ala ggt gcc gga acc Gly Ala Gly Thr cgt gaa gtg tcc Arg Giu Val Ser acc aat gag tca Thr Asn Glu Ser gtg ttg Val1 Leu 175 aca ttt tta cga gaa cac aag ggc caa acc att ttg tgt gtc aac aac Thr Phe Leu Arg Giu His Lys Giy Gin Thr Ile Leu Cys Val Asn Asn WO 01/00843 WO 0100843PCTJIBOOIOO923 atg Met gga Gly gc t Ala 225 agc Ser cac His 210 gaa Giu 180 aaa. tat cct Lys Tyr Pro 195 acc cct cga Thr Pro Arg cgg gag tgg Arg Giu Trp cag Gin gag Giu att Ile 230 ttt gat ctc acc gcc gat gaa aag gac Phe Asp Leu Thr Ala Asp Giu Lys Asp 245 ccaacacatc atc <210> 22 <211> 252 <2i2> PRT <213> Corynebacteriun giutamicum <400> 22 Thr Ala Gin Trp, Gly Ile Phe Leu Arg 1 Giu Ser Pro Leu Gly Pro Pro Ala Arg 145 Gly Thr Met Giu Leu Leu Met Met Giu Gin 130 Trp Ala Phe Val Pro Leu Ser Gly Gin Arg 115 Val1 Leu Gly Leu Ser Arg Glu Leu Asp Trp 100 Leu Asn Arg Thr Arg Giu Ala Arg 55 Ser Trp, Asp Pro Ser 135 Ile Glu Lys Arg Asn 40 Asn Pro Leu Arg Ala 120 Gin Leu Val1 Gly ctt Leu ggg Gly gcc Ala ga t Asp 250 Asn 10 Tyr Gly Leu Leu Asp Gly Gin Asn Arg Ser 170 Thr ga t Asp cag Gin cc t Pro 235 a tg Met His Met Ile Giu Tyr 75 Arg Gly Asn Arg Lys 155 Thr Ile ttg Leu ctg Leu 220 cac His gaa Giu

ASP

Tyr Arg Leu Tyr Asp Phe Asp Giu 140 Gin Asn Leu gga ttc ttc Gly Phe Phe tgagcattgg cgt Arg 205 ttc Phe Giu Ser Arg Leu G ly Gly Ser Gin 125 Asn Tyr Giu Cys Leu Gin Arg His Asp Val Lys 110 Tyr Ser Arg Ser Val1 Thr Phe Leu Gly Glu Arg Ala Gly Leu Ala Val1 175 Asn Leu Ala Ser Leu Ile Thr Asp Tyr Leu Phe 160 Leu Asn WO 01/00843 PCT/IBOO/00923 180 185 190 Met Ser Lys Tyr Pro Gin Ala Val Ser Leu Asp Leu Arg Giu Phe Ala 195 200 205 Gly His Thr Pro Arg Glu Met Ser Gly Gly Gin Leu Phe Pro Thr Ile 210 215 220 Ala Giu Arg Giu Trp Ile Val Thr Leu Ala Pro His Gly Phe Phe Trp 225 230 235 240 Phe Asp Leu Thr Ala Asp Giu Lys Asp Asp Met Glu 245 250 <210> 23 <211> 1102 <212> DNA <213> Corynebacterium giutanicun <22 0> <22i> CDS <222> (10i)..(1102) <223> RXA00891 <400> 23 tcaatattcc gaagaaaacc gcgcagctct ctcactagtc gaaagacccg ctacgcatgg tgcgcctggc tttttagaat tcaggtgagg cgaaagtggt tgg Trp tc t Ser ctt Leu gtc Val1 gag Giu tac Tyr aaa Lys ccg ttc Phe ccg- Pro gcc Al a gca Al a tac Tyr cca Pro ga t Asp tat tct Ser agt Ser cac His ccc Pro aaa Lys gaa Giu tac Tyr 105 gat atc Ile gca Ala ggc Gly gc t Ala gac Asp ggc Gly atc Ile tcc ctg Leu gat Asp ttg Leu ccc Pro gcc Ala 75 aac Asn cag Gin cc a gat

ASP

cac His gaa Giu tgg Trp gtg Val1 80 ttg Leu gat Asp ggt gtg Val 1 ac c Thr gcg Ala tcg Ser gag Glu ctg Leu aaa Lys tgc Cys tac tct Ser gct Ala gaa Glu 50 aag Lys gt t Val1 Gly att Ile gat ccg Pro cgc Arg 35 gag Giu aaa Lys cgt Arg ctg Leu tgg Trp 115 atc ctg caa acc tcc Leu Gin Thr Ser aa t Asn gtt Val1 cca Pro cgc Arg ttc Phe gaa Giu cca Pro a ac 115 16-3 211 259 307 355 403 451 499 WO 01/00843 Pro Phe Tyr 120 ttc cgt gaa Phe Arg Giu 135 ctc gtt gac Leu Val Asp 150 gtc atg aat Val Met Asn cgc gac cca Arg Asp Pro ccc acc ctg Pro Thr Leu 200 tcc aac tgg Ser Asn Trp 215 ttc ttc tcc Phe Phe Ser 230 gag gcc atg Giu Ala Met ggt ttc cga Gly Phe Arg aac ggc gaa Asn Gly Glu 280 tct gtc att 1027 Ser Val Ile 295 aac caa tgg 1075 Asn Gin Trp 310 ggc gat gaa 1102 Asp a tc Ile cac His cac His acc Thr 185 t ac Tyr acc Thr c ac His cta Leu cta Leu 265 aac Asn gag Giu ccc Pro tgc Ser ctg Leu gcc Ala acc Thr 170 ggc Gly aac Asn tat Tyr caa Gin ga t Asp 250 ga t Asp ctc Leu aag Lys caa Gin cac His 330 Pro ccc Pro cac His 155 tcc Ser ccc Pro gaa Giu ga t Asp cca Pro 235 gtc Val1 gcc Ala aaa Lys gaa Giu ga t Asp 315 atg Leu gaa Giu 140 cgc Arg gac Asp tac Tyr gcc Al a ccg Pro 220 gac Asp ttg Leu gt t Val1 gaa Glu tac Tyr 300 gtg Vali Arg 125 t tc Phe cgt Arg cag Gin gga Gly cgc Arg 205 gtg Val1 ctc Leu cgt Arg cc t Pro acc Thr 285 ccc Pro gtc Val Asp Gly ggc acc Gly Thr ggc ctg Gly Leu cac gca His Ala 175 gat ttc Asp Phe i90 atc atc Ile Ile cgt ggc Arg Gly aac tac Asn Tyr ttc tgg Phe Trp 255 tat ctt Tyr Leu 270 cac gat His Asp ggc cga Gly Arg gaa tac Giu Tyr Gly gtc Val cg t Arg 160 tgg Trp, tat Tyr ttt Phe cag Gin gac Asp 240 ctg Leu ttt Phe ttc Phe atc Ile ttc Phe 320 Tyr ga t Asp 145 gtt Val t tc Phe gtg Val gta Val1 t ac Tyr 225 aac Asn gac Asp gaa Giu ctc Leu ctg Leu 305 ggt Gly Asp 130 gac Asp a tc Ile caa Gin tgg Trp, gat Asp 210 t tc Phe ccc Pro c tg Leu c gc Arg aaa Lys 290 ctc Leu gaa Glu Ile ttc Phe acc Thr gaa Gi u agc Ser 195 ac a Thr tgg Trp gca Ala gga Gly gaa Glu 275 ctg Leu gca Ala aaa Lys PCTIIBOO/00923 Arg Asn gtg gaa 547 Val Giu gac ttg 595 Asp Leu 165 tcc cgg 643 Ser Arg 180 gat gat 691 Asp Asp gaa gaa 739 Giu Glu cac cgc 787 His Arg gtc caa 835 Val Gin 245 ctt gat 883 Leu Asp 260 ggc acc 931 Gly Thr tgt cgc 979 Cys Arg gaa gcc Glu Ala gac aaa Asp Lys 325 gcc ttc cac Gly Asp Giu Cys Met Ala Phe His <210> 24 <211> 334 WO 01/00843 PCT/IB00/00923 <212> PRT <213> Corynebacterium glutamicum <400> 24 Val Leu Gin Thr Ser Trp His Phe Ser Ile Leu Ala Gly Met Thr Asp 1 5 10 Thr Ser Pro Leu Asn Ser Gin Pro Ser Ala Asp His His Pro Asp His 25 Ala Ala Arg Pro Val Leu Asp Ala His Gly Leu Ile Val Glu His Glu 40 Ser Glu Glu Phe Pro Val Pro Ala Pro Ala Pro Gly Glu Gin Pro Trp 55 Glu Lys Lys Asn Arg Glu Trp Tyr Lys Asp Ala Val Phe Tyr Glu Val 70 75 Leu Val Arg Ala Phe Tyr Asp Pro Glu Gly Asn Gly Val Gly Ser Leu 90 Lys Gly Leu Thr Glu Lys Leu Asp Tyr Ile Gin Trp Leu Gly Val Asp 100 105 110 Cys Ile Trp Ile Pro Pro Phe Tyr Asp Ser Pro Leu Arg Asp Gly Gly 115 120 125 Tyr Asp Ile Arg Asn Phe Arg Glu Ile Leu Pro Glu Phe Gly Thr Val 130 135 140 Asp Asp Phe Val Glu Leu Val Asp His Ala His Arg Arg Gly Leu Arg 145 150 155 160 Val Ile Thr Asp Leu Val Met Asn His Thr Ser Asp Gin His Ala Trp 165 170 175 Phe Gin Glu Ser Arg Arg Asp Pro Thr Gly Pro Tyr Gly Asp Phe Tyr 180 185 190 Val Trp Ser Asp Asp Pro Thr Leu Tyr Asn Glu Ala Arg Ile Ile Phe S195 200 205 Val Asp Thr Glu Glu Ser Asn Trp Thr Tyr Asp Pro Val Arg Gly Gin 210 215 220 Tyr Phe Trp His Arg Phe Phe Ser His Gin Pro Asp Leu Asn Tyr Asp 225 230 235 240 Asn Pro Ala Val Gin Glu Ala Met Leu Asp Val Leu Arg Phe Trp Leu 245 250 255 Asp Leu Gly Leu Asp Gly Phe Arg Leu Asp Ala Val Pro Tyr Leu Phe 260 265 270 Glu Arg Glu Gly Thr Asn Gly Glu Asn Leu Lys Glu Thr His Asp Phe 275 280 285 Leu Lys Leu Cys Arg Ser Val Ile Glu Lys Glu Tyr Pro Gly Arg Ile 290 295 300 WO 01/00843 PCTIIBOO/00923 Leu Leu Ala Giu Ala Asn Gin Trp Pro Gin Asp Val Val Glu Tyr Phe 305 310 315 320 Gly Glu Lys Asp Lys Gly Asp Glu Cys His Met Ala Phe His 325 330 <210> <211> 1386 <212> DNA <213> Corynebacter.um glutamicun <220> <221> CDS <222> (101)..(1363) <223> RXA00534 <400> ctgtgcagaa agaaaacact cctctggcta ggtagacaca gcgggtaact gtcagcacgt agatcgaaag gtgcacaaag gtttataaag gtagagttga gtg gcc ctg gtc gta 11! Val Ala Leu Val Val cag Gin gtc Val gtt Val1 gca Ala ctg Leu gag Glu gtg Val cca Pro gc t Ala ggt aaa Lys gc t Ala gtc Val1 gcg Ala act Thr tcc Ser ctc Leu ggt Gly ggt Gly 135 cgt tat Tyr gaa Giu tgc Cys gca Ala gc t Al a ctt Leu acc Thr cgt Arg 120 t tc Phe gg t ggc Gly cgg Arg tcc Ser gtg Val1 gg t Gly ggc Giy acc Thr 105 gtg Val1 cag Gin ggt ggt Gly a tc Ile gca Ala aa t Asn gag Giu gca Ala gag Glu cgt Arg ggt G ly tct t cc Ser gtt Val atg Met ccc Pro cgt Arg gaa Glu cgc Arg gaa Giu gtt Val1 gac tcg Ser gcc Ala gga Gly gtt Val1 att.

Ile gcc Ala cac Hits gca Ala aat Asn 140 acc cgc Arg aat Asn ctt Leu atg Met gcc Ala tct Ser gt t Val1 atc Ile 130 gtc Val1 gca att Ile ga t Asp cta Leu ga t Asp atg Met cag Gin ga t Asp 115 t gc Cys acc Thr gc t aga Arg gtc Val1 gaa Glu a tg Met gc t Ala gct Ala i00 gtc Val att Ile acg Thr gct WO 01/00843 WO 0100843PCT/EB00100923 Gly Arg 150 aac gct Asn Ala gct gac Ala Asp ttc gaa Phe Giu ctg cgC Leu Arg 215 cgc tcg Arg Ser 230 gag gat Giu Asp aag tcc Lys Ser gag gct Giu Ala gac atg 1027 Asp Met 295 atc acc 1075 Ilie Thr 310 ttg aag 1123 Leu Lys gac cag 1171 Asp Gin cca ggt 1219 Gly Gly Ser Asp 155 gat gtg tgt gag Asp Val Cys Glu 170 ccg cgc atc gtt Pro Arg Ile Val 185 gaa atg ctg gaa Giu Met Leu Giu 200 agt gtt gaa tac Ser Val Giu Tyr tct tat agt aat Ser Tyr Ser Asn 235 att cct gtg gaa Ile Pro Val Giu 250 gaa gcc aaa gta Giu Ala Lys Val 265 gcg aag gtt ttc Ala Lys Val Phe 280 gtt ctg cag aac Val Leu Gin Asn ttc acc tgc cct Phe Thr Cys Pro 315 aag ctt cag gtt Lys Leu Gin Val 330 gtc ggc aaa gtc Vai Gly Lys Val 345 gtt acc gca gag Thr Thr Ala Vai Ala Leu Ala Ala 160 at t Ile cc t Pro ctt Leu gc t Ala 220 gat Asp gaa Giu acc Thr cg t Arg gtc Val1 300 cgt Arg cag Gin tcc Ser ttc t ac Tyr aat Asn gc t Ala 205 cgt Arg ccc Pro gca Ala gt t Val gcg Ala 285 tct Se r tcc Ser ggc Giy c tc Leu atg tcg Ser gca Ala 190 gct Ala gc a Al a ggc Gly gtc Val ctg Leu 270 ttg Leu tct Ser gac Asp aac Asn gtg Val1 350 gaa gac

ASP

175 cag Gin gtt Val1 t tc Phe act Thr ctt Leu 255 gg t Gly gc t Ala gt a Val1 Gly tgg Trp 335 ggt Gly gct ggt Gly gaa Glu aag Lys 210 cca Pro gcc Ala gtc Val1 gat Asp gaa Giu 290 ggc Gly gcg Ala gtg Val1 atg Met gat gtg Val1 aag Lys 195 att Ile ctt Leu ggc Gly gca Ala aag Lys 275 atc Ile acc Thr atg Met ctt Leu aag Lys 355 gtc Ala tat Tyr 180 C tC Leu t tg Leu cgc Arg tct Ser acc Thr 260 cca Pro aac Asn acc Thr gag Glu tac TIyr 340 tc t Ser aa c Leu 165 acc Thr agc Ser gtg Val1 gta Val1 atg Met 245 gac Asp 9gc Gly att Ile gac

ASP

atc Ile 325 gac

ASP

cac His gtg Pro Gly Val Thr Ala Giu Phe Met Glu Ala Leu Arg Asp Val Asn Val 360 365 370 WO 01/00843 WO 0100843PCT/IBOO/00923 aac atc gaa ttg att tcc acc tct gag 1267 Asn Ile Giu Leu Ile Ser Thr Ser Giu 375 380 cgt gaa gat gat ctg gat gct gct gca 1315 Arg Giu Asp Asp Leu Asp Ala Ala Ala 390 395 cag ctg. ggc ggc gaa gac gaa gcc gtc .1363 Gin Leu Gly Gly Giu Asp Giu Ala Val 410 taaagtttta aaggagtagt ttt 1386 <210> 26 <211> 421 <212> PRT <213> Corynebacterium glutamicum <400> 26 Val Ala Leu Val Val Gin Lys Tyr Gly att Ile cgt Arg gt t Val1 415 cgt att tcc gtg Arg le Ser Val 385 gca ttg cat gag Ala Leu His Giu 400 tat gca ggc acc Tyr Ala Gly Thr ctg atc Leu Ile cag ttc Gin Phe 405 gga cgc Giy Arg 420 1 Glu Gly Glu Giu Val Gly Ile Lys Asp 145 Leu Asp Arg Asn Leu Met Ala Ser Val1 Ile 130 Val1 Ala Gly Ile Asp Leu Asp Met Gin Asp 115 Cys Thr Ala Val1 5 Asn Val1 Leu Leu Ile G ly Thr Val Leu Leu 165 Thr Val1 Val Ala Leu 70 Giu Val1 Pro Ala Gly 150 Asn Ala Ala Val Ala Thr Ser Leu Gly G ly 135 Arg Ala Asp Glu Cys 40 Ala Ala Leu Thr Arg 120 Phe Gly Asp Pro Arg Ser Val1 Gly Gly Thr 105 Val1 Gin C ly Val1 Arg 185 Gly. Ser 10 Ile Val Ala Met Asn Pro Glu Arg 75 Ala Giu 90 Glu Arg Arg Giu Gly Val Ser Asp 155 Cys Giu 170 Ile Val Leu Thr Asp Pro Ser Gin Gly Leu 125 Lys Thr Tyr Asn Glu Lys Thr Pro Asn Ser Asn 110 Asp Giu Ala Ser Ala 190 Ser Lys Thr Al a Ala Phe Ala Giu Thr Val1 Asp 175 Gin Ala Ala Asp Arg Leu Thr Arg Gly Arg Ala 160 Val1 Lys WO 01/00843 WO 0100843PCT/IBOO/00923 Leu Glu Lys Leu Ser Phe Glu Glu Met Leu Glu LeU Ala Ala Val Giy 195 200 205 Ser Lys Ile Leu Val.Leu Arg Ser Val Glu Tyr Ala Arg Ala Phe Asn 210 215 220 Val Pro Leu Arg Vai Arg Ser Ser Tyr Ser ASn Asp Pro Gly Thr Leu 225 230 235 240 Ile Ala Gly Ser Met Glu Asp Ile Pro Val Glu Glu Ala Val Leu Thr 245 250 255 Giy Val Ala Thr Asp Lys Ser Glu Ala Lys Val Thr Val Leu Gly Ile 260 265 270 Ser Asp Lys Pro Gly Giu Ala Ala Lys Val Phe Arg Ala Leu Ala Asp 275 280 285 Ala Glu Ilie Asn Ile Asp Net Val Leu Gin Asn Val Ser Ser Val Giu 290 295 300 Asp Gly Thr Thr Asp Ilie Thr Phe Thr Cys Pro Arg Ser Asp Gly Arg 305 310 315 320 Arg Ala Met Giu Ile Leu Lys Lys Leu Gin Vai Gin Giy Asn Trp Thr 325 330 335 Asn Val Leu Tyr Asp Asp Gin Val Gly Lys Vai Ser Leu Val Giy Ala 340 345 350 Gly Met Lys Ser His Pro Gly Val Thr Ala Glu Phe Met Giu Aia Leu 355 360 365 Arg Asp Val Asn Vai Asn Ile Giu Leu Ile Ser Thr Ser Glu Ile Arg 370 375 380 Ile Ser Vai Leu Ile Arg Glu Asp Asp Leu Asp Ala Ala Ala Arg Ala 385 390 395 400 Leu His Glu Gin Phe Gln Leu Gly Gly Giu Asp Giu Ala Val Val Tyr 405 410 415 Ala Gly Thr Gly Arg 420 <210> 27 <2i1> 1155 <212> DNA <213> Corynebacterium giutamicun <22 0> <221> CDS <222> (101)..(1132) <223> RXA00533 <400> 27 ctgcacgtgc attgcatgag cagttccagc tgggcggcga agacgaagcc gtcgtttatg caggcaccgg acgctaaagt tttaaaggag tagttttaCa atg acc acc atc gca 115 Met Thr Thr Ile Ala WO 01/00843 WO 0100843PCTA~BOO/00923 1 gtt gtt ggt gca acc ggc cag gtc ggc cag gtt atg cgc acc Val Val Gly Ala Thr Gly-Gin Val Gly gaa Glu cgt Arg gaa Glu gcg Ala t tc Phe aag Lys aag Lys atg Met gta Val1 150 gca Ala aac Asn gga Gly aac Asn gag Giu tcc Ser gac Asp t tg Leu gct Ala gac Asp gat Asp gc t Ala 135 aag Lys ggt Gly gt t Val1 cct Pro ctc Leu 215 cgc Arg gca Ala att Ile ttc Phe gc t Ala gac Asp t cc Ser 120 gcg Al a ctt Leu gtg Val1 gag Giu tat Tyr 200 gtc Val1 aat Asn ggc Gly act Thr tcc Ser gca Ala gag Giu 105 ctg Leu atg Met cac His gaa Giu ttc Phe 185 gtt Val1 ga t Asp ttc Phe cgt Arg cag Gin gc t Aia ggc G ly gtt Val gtc Val1 cca Pro gt t Val1 acc Thr 170 gtc Val tca Ser gac Asp cca Pro aag Lys gca Ala gga Gly 75 gcg Ala cca Pro aag Lys gtg Val tcc Ser 155 t tg Leu cat His cca Pro ggc Gly Gin Val Met 15 gtt cgt ttc Val Arg Phe cgt ggc acg Arg Gly Thr tcc ctc aag Ser Leu Lys tcc aag cag Ser Lys Gin gat aac tct Asp Asn Ser 95 tct gag gtg Ser Glu Val gcg aac cct Ala Asn Pro ctt cac gat Leu His Asp 145 gct gtt tcc Ala Val Ser 160 gtt gct gca Vai Ala Ala 175 gct gct gac Ala Ala Asp aac gtg ctg Asn Val Leu acc gat gaa Thr Asp Giu 225 ctc cca gac Leu Pro Asp 240 Arg ttt Phe gaa Giu gac Asp tac Tyr tc t Ser aac Asn aac Asn 130 gcc Ala gg t G ly gtt Val1 gca Ala cca Pro 210 gag Glu ctc Leu Thr gc t Aia atc Ile atc Ile gc t Ala gct Ala cct Pro 115 tgc Cys gc t Ala tc t Ser gga G iy G ly 195 t tc Phe cag Gin aag Lys ctt Leu tcc Ser gag Glu gac Asp cca Pro tgg Trp 100 tcc Ser acc Thr ggt Giy ggt G ly gac Asp 180 gat Asp gcc Ala aag Lys gtc Val1 cgc aac gaa Arg'Asn Giu 230 tcc cgc aag att ctc ggt Ser Arg Lys Ile Leu Gly 235 WO 01/00843 WO 0100843PCTIiBOO/00923 ggc acc Gly Thr cac gcc His Ala ttg ggt Leu Gly gca gct 1027 Ala Ala 295 tcc act 1075 Ser Thr 310 aac ctc 1123 Asn Leu ctg gtt 1155 Leu Val gtc cgc gtg ccg gtt ttc ac Val Arg Val Pro Val Phe Thr 250 255 ttc gac aag gca atc acc gtg Phe Asp Lys Ala Ile Thr Val 265 -270 gct tca ggc gtc aag ctt gtc Ala Ser Gly Val Lys Leu Val 285 ggc att gac gaa tcc ctc gtt Gly Ilie Asp Glu Ser Leu Val 300 gac gat aac cgc ggt ctg gtt Asp Asp Asn Arg Gly Leu Val 315 aag ggt gct gcg cta aac acc Lys Gly Ala Ala Leu Asn Thr 330 335 taaaaacccg ccattaaaaa ctc ctg Leu cag Gin 275 acc Thr cgt Arg tct Ser gc t Ala Gly Thr Phe Glu Al a Val Val 110 att Ile a tc Ile ctt Leu gac Asp gac Asp 325 ctg Leu <210> 28 <211> 344 <212> PRT <213> Corynebacteriun giutamicum <400> 28 Met Thr Thr Ile Ala Val Val Gly Ala 1 Met Arg Thr Leu Leu Giu Giu Arg Asn 25 Phe Phe Ala Ser Pro Arg Ser Ala Gly Thr Giu Ile Giu Val Glu Asp Ile Thr Lys Asp Ile Asp Val Ala Leu Phe Ser Gin Tyr Ala Pro Leu Phe Ala Ala Ala Ser Ser Ala Trp Arg Lys Asp Asp Glu 100 105 WO 01/00843 WO 0100843PCTIIBOO/00923 Val Pro Asp 145 Ser Ala Asp Leu Giu 225 Asp His Gin Val Arg 305 Val1 Gin Asn Asn 130 Ala G ly Val Ala Pro .210 Giu Leu Thr Ala Pro 290 Ile Val Ile Pro 115 Cys Ala Ser Giy Gly 195 Phe Gin Lys Leu Gin 275 Thr Arg Se r Ala Ser Asp Lys Asp Ser Leu Vai Lys Gly Ile Ilie Ala Asn 120 125 Thr Gly Gly Asp 180 Asp Ala Lys Val1 Thr 260 Giu Pro Gin Gly Glu 340 Thr Leu Leu 165 His Val1 Gly.

Leu Ser 245 Ile Ile Leu Asp Asp 325 Leu Met Vali 150 Ala Asn Gly Asn Arg 230 Gly His Leu Ala Ser 310 Asn Leu Ala 135 Lys Gly Val1 Pro Leu 215 Asn Thr Ala G ly Al a 295 Thr Leu Val Ala Leu Val1 Glu Tyr 200 Val1 Giu Cys Giu Ala 280 Ala Val1 Arg Lys Met His Glu Phe 185 Val1 Asp Ser Val Phe 265 Ala Gly Asp Lys Pro Val Thr 170 Val1 Ser Asp Arg Arg 250 Asp Ser Ile Asp G ly 330 Val Ser 155 Leu His Pro Gly Lys 235 Val1 Lys Gly Asp Asn 315 Ala Leu 140 Ser Ala Asp Ile Thr 220 Ile Pro Ala Val Giu 300 Arg Ala

LYS

Tyr Lys Gly Ala 205 Phe Leu Val Ile Lys 285 Ser Gly Leu Pro Gin Gin Gin 190 Tyr Glu Gly Phe Thr 270 Leu Leu Leu Asn Leu Ala Val 175 Ala Asn Thr Leu Thr 255 Val Val1 Val1 Val1 Thr 335 His Val1 160 Ala Al a Val1 Asp Pro 240 Gly Asp Asp Gly Leu 320 Ile <210> 29 <211> 608 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (69)..(608) <223> RXA02843 <400> 29 cccattgcgc ggaggtcgca ccccttccga cttgaactga taggccgata gaaattattc tggacgtcatg act act gct tcc gca acc gga att gca aca ctg acc tcc 110 Met Thr Thr Ala Ser Ala Thr Gly Ile Ala Thr Leu Thr Ser 42 WO 01/00843 WO 0100843PCT/IBOO/00923 1 5 acc ggc gac gtc ctg gac gtg tgg tat cca gaa atc ggg tcc acc gac 158 Thr Giy Asp Val Leu Asp Val Trp Tyr Pro Glu Ile Gly Ser Thr Asp 20 25 cag tcc gcg ctc aca cct cta gaa ggc gtc gat gaa gat cga aac gtc 206 Gin Ser Ala Leu Thr Pro Leu Giu Giy Val Asp Glu Asp Arg Asn Val 40 acc cgc aaa atc gtg acg aca act atc gac acc gac gca gcc ccc acc 254 Thr Arg Lys Ile Vai Thr Thr Thr Ilie Asp Thr Asp Ala Ala.Pro Thr 55 gac acc tac gat gca tgg ctg cgc ctt cac ctc ctc tcc cac cgc gtt 302 Asp Thr Tyr Asp Aia Trp Leu Arg Leu His Leu Leu Ser His Arg Vai 70 ttc cgc cct cac acc atc aac cta gac ggc att ttc ggc ctc ctc aac 350 Phe Arg Pro His Thr Ile Asn Leu Asp Giy Ile Phe Giy Leu Leu Asn 85 aat gtc gtg tgg acc aac ttc gga ccg tgc gca gtt gac ggt ttc gca 398 Asn Val Val Trp Thr Asn Phe Giy Pro Cys Ala Val Asp Gly Phe Ala 100 105 110 ctc acc cgc gcg cgc ctg tca cgc cga ggc caa gtt acg gtt tat agc 446 Leu Thr Arg Ala Arg Leu Ser Arg Arg Gly Gin Val Thr Val Tyr Ser 115 120 125 gtc gac aag ttc cca cgc atg gtc gac tat gtg gtt ccc tcg ggc gtg 494 Val Asp Lys Phe Pro Arg Met Val Asp Tyr Val Val Pro Ser Gly Val 130 135 140 cgc atc ggt gac gcc gac cgc gtc cga ctt ggc gcg tac ctg gca gat 542 Arg Ilie Gly Asp Ala Asp Arg Val Arg Leu Gly Ala Tyr Leu Ala Asp 145 i50 ggc acc acc gtg atg cat gag ggc ttc gtg aac ttc aac gct ggc acg 590 Gly Thr Thr Val Met His Giu Gly Phe Vai Asn Phe Asn Aia Giy Thr 160 165 170 ctc ggc gct tcc atg gtt 608 Leu Gly Ala Ser Met Val 175 180 <210> <211> 180 <212> PRT <213> Corynebacteriun giutanicuxn <400> Met Thr Thr Ala Ser Ala Thr Gly Ilie Ala Thr Leu Thr Ser Thr Gly 1 5 10 Asp Val Leu Asp Val Trp Tyr Pro Glu Ile Gly Ser Thr Asp Gin Ser 25 Ala Leu Thr Pro Leu Glu Giy Val Asp Giu Asp Arg Asn Vai Thr Arg 40 WO 01/00843 WO 0100843PCT/BOOIOO923 Lys Tyr Pro Val1 Arg Lys Gly 145 Thr Ala Thr Trp, Ile Asn 100 Leu Arg Asp His Val1 180 Ile Leu Asp Pro Arg Asp 135 Arg Phe Asp His Gly Cys G ly 120 Tyr Leu Val1 Asp Leu Phe Val1 Val1 Val1 Ala Phe 170 Ala Ser 75 Gly Asp Thr Pro Tyr 155 Asn Thr Val1 Asn Ala 110 Ser Val1 Asp Thr Asp Phe Asn Leu Val1 Arg G ly Leu 175 Thr Arg Va 1 Thr Asp Ile Thr 160 Gly <210> 31 <211> 1230 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1207) <223> RXA02022 <400> 31 tatttgcgat tccaactgct tgggctccgc gaatgttttc cttccatcat gttttaacta aggtttgtag gcttaaacct actcattttt taatcgaccg gtg aac tct. gaa ctc ctc Ctc ggc Leu Leu Gly ccg agt Pro Ser gcc ctt Ala Leu aac aac Asn Ashl gac Asp ggt Gly ctt Leu Val Asn Ser Giu Leu 1 att gtc ctt act caa cgt Ile Val Leu Thr Gin Arg gaa aag cag att gct gat Glu Lys Gin Ile Ala Asp cta cct ggt gta gag gtc Leu Pro Gly Val Giu Val acg aac agg gga ttg gcc Thr Asn Arg Gly Leu Ala gct cgc Ala Arg WO 01/00843 WO 0100843PCTIIBOO/00923 tcg agg Ser Arg aat ctg Asn Leu gtc gat Val Asp ttg gcc Leu Ala gag tgc Glu Cys 135 gat gag Asp Glu 150 cct act Pro Thr aag gtg Lys Val ggt gat Gly Asp gcg tat Ala Tyr 215 ggc ctc Gly Leu 230 cca gac Pro Asp gat ctc Asp Leu ggt caa Gly Gin ctt cca 1027 Leu Pro 295 gtc Val1 cca Pro atg Met acg Thr 120 gag Glu cat His 9gC G ly acg Thr aa t Asn 200 aag Lys aa c Asn ctc Leu aac Asn gac.

Asp 280 ggc Gly atg Met agc Ser aaa Lys 105 tcg Ser gaa Glu ccg Pro 9gc Gly gcg Ala 185 gcg Ala gcc Ala atc Ile gcg Ala gag Giu 265 ggC Gly t tg Leu ctt Leu cgt Arg tc t Ser act Thr gt t Val1 gag Giu tgg Trp 170 cat His atg Met gca Ala gtt Val1 tgg Trp 250 gcg Ala atc Ile ggg Gly gc t Ala 75 gtg Val1 9gg Gly gag Glu gct Ala tgg Trp 155 att Ile ggt Gly cat His gaa Giu ttc Phe 235 atg Met atc Ile gaa Glu cag Gin gg t Gly gaa Glu t tg Leu ctt Leu gat Asp 140 t tg Leu gag Giu gtg Val aag Lys gtc Val1 220 tgc Cys aac Asn gag Glu t gg Trp cag Gin 300 cat His gac Asp gcg Ala aaa Lys 125 cac His gcg Ala gcg Ala cgt Arg t tg Leu 205 aac Asn gaa Giu c tc Leu cat His gcc Ala 285 gtg Val atc Ile ggc Gly gtg Val1 110 cat His ctc Leu gc t Ala ggc Gly gcc Ala 190 tcg Ser att Ile tcg Ser aac Asn gtc Val1 270 gta Val1 aca Thr gat Asp a tc Ile 95 tat Tyr gat Asp aa t Asn gat Asp tgc Cys 175 cat His ccg Pro ga t Asp ggc Gly t tc Phe 255 gtc Val1 gaa Glu agc Ser aca gtg Thr Val atg tat Met Tyr ttg cat Leu His ctg acg Leu Thr ggt ttg Gly Leu 145 ttg gcg Leu Ala 160 cag ggc Gin Gly tcg gcg Ser Ala atc att Ile Ile ggc ttg Gly Leu 225 gtg gca Val Ala 240 cgt ttc Arg Phe gaa acg Giu Thr gac ggg Asp Gly ggg ctt Gly Leu 305 ccg Pro ggC Gly act Thr ctg Leu 130 ggC G ly ttg Leu aa t Asn aga Arg t cg Ser 210 acc Thr aac Asn gcg Ala ctt Leu gca Ala 290 a tc Ile atc Ile tgt Cys ttt Phe 115 att Ile cac His ttg Leu c tg Leu agc Ser 195 aag Lys tac Tyr aac Asn ccg Pro gag Glu 275 ggC Gly gac Asp gcg Ala ggC G ly 100 gcc Ala 6cg Ala att Ile ggt Gly cgc Arg 180 tgg Trp gtt Val cgt Arg gtc Val1 aat Asn 260 ctt Leu ggt Gly gcc Ala 355 403 451 499 547 595 643 691 739 787 835 883 931 979 WO 01/00843 WO 0100843PCTLBOO/00923 ggc cgc gaa aaa atc cgc gca aaa ttc 1075 Gly Arg Glu Lys Ile Arg Ala Lys Phe 310 315 ttt tca gcc atg gga att cca gcc cta 1123 Phe Ser Ala Met Gly Ile Pro Ala Leu 330 agt ttc gcg cat aaa cgc gac gag cag 1171 Ser Phe Ala His Lys Arg Asp Glu Gin 345 350 gat gtg gca gca att ttg aag cag tac 1217 Asp Val Ala Ala Ile Leu Lys Gin Tyr 360 365 cggggttatc gtg 1230 <210> 32 <211> 369 <212> PRT <213> Corynebacteriun giutamicum <400> 32 Val Asn Ser Giu Leu Lys Pro Gly Leu ggc Gly aac Asn 335 tgc Cys ctg Leu Asp 10 Pro Ala Asn Leu Arg 90 Ser Thr Val1 Giu tgg Trp 320 ttt Phe cca Pro agc Ser Leu Ser Leu Asn Ala 75 Val1 Gly Giu Ala Trp 155 acc gat gtc tca Thr Asp Val Ser ggc gct ggt gat Gly Ala Gly Asp 340 gtg gag caa atc ValiGlu Gin Ile 355 gag taaccgcatt Glu cgt Arg 325 cca Pro acg Thr Ile Giu Leu Thr Thr Met Leu Leu Gly Leu 160 1 Val1 Lys Pro Asn Val1 Tyr His Thr Leu 145 Leu Gin Gly Arg Pro Gly Thr Leu 130 Gly Gin Ala Giu Leu Al a Giy 100 Ala Ala Ile 5 Arg Asp Val Ala Asp Thr Thr Tyr Arg Val1 Ile Arg 55 Arg Leu Asp Ala Cys 135 Glu Leu Pro Arg Val1 Gly Glu Leu Leu Asp 140 Leu Asp Gly Leu Ala Ile Gly Val 110 His Leu Ala Pro Gin Asn Arg Asp Ile Tyr Asp Asn Asp WO 01/00843 WO 0100843PCTfIB00/00923 Ala Leu Leu Gly Giu Pro Thr Gly Gly Trp Ile Giu Ala Gly Cys Gin 165 170 175 Gly Asn Leu Arg Ile Lys Val Thr Ala His Gly Val Arg Ala His Ser 180 185 190 Ala Arg Ser Trp Leu Gly Asp Asn Ala Met His Lys Leu Ser Pro Ile 195 200 205 Ilie Ser Lys Val Ala Ala Tyr Lys Ala Ala Giu Val Asn Ile Asp Gly 210 215 220 Leu Thr Tyr Arg Giu Gly Leu'Asn Ile Val Phe Cys Giu Ser Gly Val 225 230 235 240 Ala Asn Asn Val Ilie Pro Asp Leu Ala Trp Met Asn Leu Asn Phe Arg 245 250 255 Phe Ala Pro Asn Arg Asp Leu Asn Glu Ala Ile Giu His Val Val Giu 260 265 270 Thr Leu Giu Leu Asp Gly Gin Asp Gly Ilie Giu Trp Ala Val Giu Asp 275 280 285 Gly Ala Gly Gly Ala Leu Pro Gly Leu Gly Gin Gin Val Thr Ser Gly 290 295 300 Leu Ile Asp Ala Val Gly Arg Giu Lys Ile Arg Ala Lys Phe Gly Trp 305 310 315 320 Thr Asp Val Ser Arg Phe Ser Ala Met Gly Ile Pro Ala Leu Asn Phe 325 330 335 Gly Ala Gly Asp Pro Ser Phe Ala His Lys Arg Asp Giu Gin Cys Pro 340 345~ 350 Val Giu Gin Ile Thr Asp Val Ala Ala Ile Leu Lys Gin Tyr Leu Ser 355 360 365 Giu <210> 33 <211> 1059 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS <222> (1036) <223> RXA00044 <400> 33 attacctcag ccttccaagc tgatgatgca ttacttaaaa actgcagaca cttgaaaaac ttctcacccg cactcgttcc ctcaacccac aaggagcacc atg gct tcc gca act 115 Met Ala Ser Ala Thr 1 ttc acc ggc gtg atc cca ccc gta atg acc cca ctc cac gcc gac ggc 163 47 WO 01/00843 Phe Thr Gly Val Ile Pro Pro Val Met Thr Pro Leu His Ala 15 ag t Ser ggt Gly ttc Phe cac His act Thr gaa Giu gaa Giu cca Pro cca Pro 150 aag Lys cgt Arg gaa Giu cca Pro C tC Leu 230 atc Ile gtg Val1 Gly ctc Leu acc Thr gct Ala ggc Gly at t Ile ctg Leu 135 gtc Val gat Asp ga t Asp acc Thr ggC Gly 215 tgc Cys aac Asn gat

ASP

gtc Val1 acc Thr gca Ala cgc Arg ctc Leu gaa Giu 120 ttt Phe atg Met tcc Ser ga t Asp acc Thr 200 c tg Leu C tC Leu cac His gta gaa Val Giu gac gga Asp Gly cgc gcC Arg Ala ggc cgc Gly Arg gtg att Val Ilie gtt gcc Val Ala 105 gaa cac Giu His gcc tac Ala Tyr ctt ttg Leu Leu agt ggc Ser Gly 170 gct gga Ala Gly 185 gtt gat Val Asp ggc aat Gly Asn gat gga Asp, Gly ctc ttc Leu Phe agc Ser ctt Leu c ag Gin gtt Val1 75 gag Giu act Thr ttc Phe aac Asn acg Thr 155 aat Asn ctc Leu ttc Phe gt t Val1 aag Lys 235 ca c His ctc Leu ttc Phe cgc Arg 60 ccc Pro ctc Leu gca Ala cgC Arg atc Ile 140 ctg Leu gat Asp act Thr gCC Ala gat Asp 220 tgg Trp a tc Ile cgc Arg gca Ala 45 aaa Lys gta Val1 gtg Val1 cct Pro aag Lys 125 cca Pro gCC Ala ggc Gly gag Giu tac Tyr 205 CC t Pro gca Ala gtc Val aag Lys 30 ctg Leu ctc Leu act Thr gaa Giu ttc Phe 110 atc Ile gtg Val aag Lys gca.

Ala cag Gin 190 ctt Leu gc a Ala gaa Giu ttc Phe 48 ctc Leu 9gC Gly gca Ala gct Ala gat Asp 95 tac Tyr cac His tcg Ser gat Asp atc Ile 175 ttc Phe gcg Ala gca Ala gct Ala gt t Val1 tcc Ser c tg Leu ggt Gly 80 gcc Ala acc Thr gcc Al a gtg Val1 ggc Gly 160 cgc Arg aag Lys ggt G ly tac Tyr gc t Ala 240 gac Asp tca Ser acc Thr gtc Val ctg Leu cgc Arg gcc Ala cac His 145 gtt Val tca Ser atc Ile gcc Ala gca Ala 225 gc t Ala cac His ggC Gly acc Thr att Ile gag Giu acc Thr gc t Ala 130 tcc Ser ctt Leu ctg Leu ctc Leu gat Asp 210 gct Ala t tg Leu ctc Leu gaa Glu atc Ile gaa Giu gct Ala cac His 115 cca Pro aac Asn gc a Al a atc Ile ac c Thr 195 gga Gly tta Leu cag Gin PCTIIBOO/00923 ASP Gly atc aat 211 le Asn gcg gca 259 Ala Ala atc gag 307 Ile Giu acc acc 355 Thr Thr ggt gcc 403 Gly Ala 100 gat gtg 451 Asp Val gag ctt 499 Giu Leu ctc aac 547 Leu Asn ggc acc 595 Gly Thr 165 gaa gct 643 Giu Ala 180 ggc agc 691 Gly Ser gtt gtc 739 Val Val gca aaa 787 Ala Lys aag cgc 835 Lys Arg 245 gtg gga gac acc tcc cat atg Val Gly Asp Thr Ser His Met WO 01/00843 250 2 tcc gga tcc agc gct ggt ttg ggc ggt Ser Gly Ser Ser Ala Gly Leu Gly Gly 265 270 ctt ggc att att gaa tcc aat gcg atg Leu Gly Ilie Ile Glu Ser Asn Ala Met 280 285 agc gac gaa gaa act gct cgc att cac 1027 Ser Asp Glu Glu Thr Ala Arg Ile His 295 300 tac acc gct taaggcccac acctcatgac t~ 1059 Tyr Thr Ala 310 <210> 34 <211> 312 <212> PRT <213> Corynebacterium giutamicun <400> 34 Met Ala Ser Ala Thr Phe Thr Gly Val ti

P]

A

A

PCTIBOO/00923 260 aag aca gca ctc gca cac 931 Lys Thr Ala Leu Ala His 275 gtt cct cac cag agc ctc 979 Val Pro His Gin Ser Leu 290 att gtt gat gaa ttc ctg Ile Val Asp Giu Phe Leu 305 1 Leu Asp Ser Thr Val1 Leu Arg Ala His 145 Val1 Ser Ser Gly Phe His 70 Thr Glu Giu Pro Pro 150 Lys Arg Val1 Gly Leu Thr Ala Gly Ile Leu 135 Val1 Asp Asp Ile 10 Glu Gly Ala Arg Ile Ala His Tyr Leu Gly 170 G ly Pro Ser Leu Gin Val1 75 Giu Thr Phe Asn Thr 155 Asn Leu Val1 Arg Ala Lys Val1 Val1 Pro Lys 125 Pro Ala Gly Glu Met Lys Leu Leu Thr Giu Phe 110 Ile Val Lys Ala Gin Thr Leu Gly Ala Ala Asp Tyr His Ser Asp Ile 175 Phe Pro Val1 Ser Leu Gly Ala Thr Ala Val1 Gly 160 Arg Lys WO 01/00843 WO 0100843PCTIBOO/00923 180 185 Ile Leu Thr Gly Ser Giu Thr Thr Val Asp Phe 195 200 Ala Asp Gly Val Val Pro Gly Leu Gly Asn Val 210 215 Ala Ala Leu Ala Lys Leu Cys Leu Asp Gly Lys 225 230 235 Ala Leu Gin Lys Arg Ile Asn His Leu Phe His 245 250 Asp Thr Ser His Met Ser Gly Ser Ser Ala Gly 260 265 Thr Ala Leu Ala His Leu Gly Ile Ile Glu Ser 275 280 Pro His Gin Ser Leu Ser Asp Giu Giu Thr Ala 290 295 Val Asp Giu Phe Leu Tyr Thr Ala 305 310 <210> <211> 867 <212> DNA <213> Corynebacterium giutarnicum <220> <221> CDS <222> (101)..(844) <223> RXA00863 <400> aacggtcagt taggtatgga tatcagcacc ttctgaacgg gtttgaaaaa ctcttcgccc cacgaaaatg aaggagcata 190 Leu Ala Glu Phe Gly 270 Met His Ala Ala Ala Val1 255 Phe Ala Ala ggc gtt Gly Val gtc aat Val Asn gat gat Asp Asp ttc acc Phe Thr aac ggc ctc Leu gag Giu ttg Leu act Thr att gcc Ala gac Asp ctt Leu aac Asn gcg gtacgtctag actggtgggc atg gga atc aag gtt Met Gly Ile Lys Val 1 act att gtg gca gca Thr Ile Val Ala Ala gag atc ggc gtc gac Glu Ile Gly Val Asp gaa gtt gtc gtt gac Glu Val Val Val Asp gag ttc tgc atc aac Giu Phe Cys Ile Asn ttc gat gat gct cgt WO 01/00843 Asn Gly Ilie ttg gag cag Leu Glu Gin ctg atc gca Leu Ile Ala tcc aag cag Ser Lys Gin 120 cac cac ccc His His Pro 135 gct cag ggc Ala Gin Giy 150 cca gat gcg Pro Asp Ala gat gga atc Asp Gly Ile gag caa gtt Giu Gin Val 200 gac tcc tat Asp Ser Tyr 215 cgc aac att Arg Asn Ile 230 cta ggc ctg Leu Gly Leu PCT/iBOO/00923 Ser Ala Val Val Gly Thr Thr 75 gtt Val1 cct Pro 105 gc t Ala aac Asn att Ile acc Thr ccg Pro 185 atc Ilie gat Asp gca cgc Arg aac Asn gcc Ala aag Lys gct Ala gag Giu 170 gtt Vai ttt Phe cgc Arg cag gac Asp ttt Phe cgc Arg ctg Leu gcg Ala 155 cag Gin cat His ggc Gly aac Asn cac tgg Trp gc t Ala ttc Phe ga t Asp 140 gca Ala gca Ala gca Ala acc Thr tca Ser 220 cca ctt Leu a tc Ile t tc Phe 125 gca Ala cgc Arg ctt Leu gtC Val1 cag Gin 205 ttt Phe 99c gaa Giu tct Ser 110 gaa Glu cct Pro aaa Lys gag Giu cgc Arg 190 ggt G ly gca Ala cta gga Gly 95 gcg Ala tca Ser tca Ser gaa Giu gg t Gly 175 atg Met cag Gin cca Pro gtc Gly 80 aaa Lys gtg Val gc t Ala ggc Gly gca Ala 160 tcc Ser tcc Ser acc Thr ggt Gly gta Val 240 Phe Asp Asp Ala gac aat gtc ggt Asp Asn Val Gly 100 ttg acc atg gtc Leu Thr Met Val 115 gaa gtt att gag Giu Val Ile Glu 130 acc gcg atc cac Thr Ala Ile His 145 ggc atg gac gca Gly Met Asp Ala cgt ggc gca agc Arg Gly Aia Ser 180 ggc atg gtt gct Gly Met Val Ala 195 ttg acc atc aag Leu Thr Ile Lys 210 gtc ttg gtg ggt Val Leu Val Gly 225 gga ctt gag cat Gly Leu Glu His Arg gtt Val ttt Phe ctg Leu act Thr cag Gin 165 gta Val cac His cag Gin gtg Val tac Tyr 245 403 451 499 547 595 643 691 739 787 835 Ala Gin His Pro Gly Leu Val 235 taaaggctca tttcagcagc ggg <210> 36 <21i> 248 <212> PRT <213> Corynebacterium glutaricumn <400> 36 Met Gly Ile Lys Vai Gly Val Leu Gly Ala Lys Gly Arg Val Gly Gin 1 5 10 Thr Ile Val Ala Ala Val Asn Giu Ser Asp Asp Leu Giu Leu Val Ala 25 Glu Ilie Gly Val Asp Asp Asp Leu Ser Leu Leu Val Asp Asn Gly Ala 51 WO 01/00843 WO 0100843PCTLBOO/00923 40 Giu Val Val Val Asp Phe Thr Thr Pro Asn Ala Val Met Gly Asn Leu 55 Giu Phe Cys Ile Asn Asn Gly Ilie Ser Ala Vai Val Giy Thr Thr Giy 70 75 Phe Asp Asp Ala Arg Leu Giu Gin Val Arg Asp Trp Leu Giu Gly Lys 90 Asp Asn Val Gly Val Leu Ile Ala Pro Asn Phe Ala Ile Ser Ala Val 100 105 110 Leu Thr Met Val Phe Ser Lys Gin Ala Ala Arg Phe Phe Giu Ser Ala 115 120 125 Giu Vai Ilie Glu Leu His His Pro Asn Lys Leu Asp Ala Pro Ser Gly 130 135 140 Thr Ala Ile His Thr Aia Gin Giy Ile Ala Ala Aia Arg Lys Giu Ala 145 150 155 160 Gly Met Asp Ala Gin Pro Asp Ala Thr Giu Gin Aia Leu Giu Gly Ser 165 170 175 Arg Giy Ala Ser Vai Asp Giy Ilie Pro Vai His Aia Val Arg Met Ser 180 185 190 Gly Met Val Ala His Giu Gin Vai Ile Phe Giy Thr Gin Giy Gin Thr 195 200 205 Leu Thr Ilie Lys Gin Asp Ser Tyr Asp Arg Asn Ser Phe Ala Pro Gly 210 215 220 Vai Leu Val Giy Val Arg Asn Ile Ala Gin His Pro Giy Leu Val Val 225 230 235 240 Giy Leu Giu His Tyr Leu Giy Leu 245 <210> 37 <211> 873 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (850) <223> RXA00864 <400> 37 acagcaccca ggcctagtcg taggacttga gcattaccta ggcctgtaaa ggctcatttc agcagcgggt ggaatttttt aaaaggagcg tttaaaggct gtg gcc gaa caa gtt 115 Vai Ala Giu Gin Vai 1 aaa ttg agc gtg gag ttg ata gcg tgc agt tct ttt act cca ccc gct 163 Lys Leu Ser Val Giu Leu Ile Ala Cys Ser Ser Phe Thr Pro Pro Ala 52 WO 01/00843 WO 0100843PCTIiBO0/00923 ga t Asp ttt Phe act Thr act Thr tct Ser tc t Ser gtg Val1 cac His ctg Leu 150 cag Gin aga Arg atg Met gaa Glu ttt Phe 230 gt t Val gcg Ala gc t Ala gc t Ala cgg Arg caa Gln ccc Pro gcc Ala 135 gaa Glu gct Ala atc Ile cga Arg tgt Cys 215 gag Glu.

gag Glu gg t Gly tcc Se r ttg Leu tcC Ser ctg Leu act Thr 120 atg Met gaa Glu cg t Arg gtg Val1 gcc Ala 200 tta Leu att Ile tgg Trp cgt Arg aat Asn ctt Leu gcg Ala tct Ser 105 ctc Leu gat Asp aaa Lys caa Gin gtg Val1 185 ag t Ser aga Arg gaa Glu tca act Ser Thr gcc tgc Ala Cys gct gcg Ala Ala gag cat Giu His 75 acc cat Thr His cag cgt Gin Arg atc gat Ilie Asp gag tct Giu Ser ctt ggc Leu Giy 155 gca gct Ala Ala 170 tct gga Ser Giy gaa cat Giu His aag ctg Lys Leu act ttg Thr Leu 235 gat Asp t ac Tyr tat Tyr 60 gcc Ala gaa Glu ttc Phe gaa Giu Arg 140 ga t Asp cgc Arg aac Asn gca Ala cag Gin 220 gca gt t Val1 gaa Giu 45 ctg Leu aat Asn t tg Leu gtg Val1 ga t Asp 125 ttc Phe gaa Glu gct Ala t tc Phe gac Asp 205 gta Val gac gag Giu 30 act Thr cgc Arg gcc Ala gtc Val cac His 110 ccg Pro gc t Ala ccg Pro gtg Val1 cgc Arg 190 gtc Val1 gca Ala gga Gly 15 ggc Gly ttt Phe cac His acg Thr cga Arg 95 agc Ser cag Gin t tc Phe aat Asn ctg Leu 175 acc Thr gaa Giu gcg Ala t cg gcg Ala gat Asp atc Ile atg Met cac His gga Gly t tg Leu aa t Asn gca Ala 160 ccc Pro tgg Trp atc Ile cca Pro caa gaa Giu aag Lys a tg Met 65 tat Tyr cgc Arg gaa Giu cgt Arg gag Glu 145 ctt Leu aac Asn agg Arg cgc Arg act Thr 225 atg gca Ala ccg Pro 50 gaa Glu atc Ile cat His tcg Ser gaa Glu 130 ctg Leu tta Leu gct Ala cat His gaa Glu 210 gtt Val gca Ala gtc Val cc t Pro ggg Gly ggc Gly tcc Ser 100 gta Val ttc Phe aa t Asn aaa .Lys gag Glu 180 at t Ile gcg Ala ggt Gly 211 259 307 355 403 451 499 547 595 643 691 739 787 835 aca agc ccg Thr Ser Pro Ala Asp Ser Gin Met 240 tat gtc Tyr Val atg gac ttt taacgcaaag ctcacaccca cga 873 Met Asp Phe 250 WO 01/00843 WO 0100843PCT/IBOO/00923 <210> 38 <211> 250 <212> PRT <213> Corynebacterium giutamicun <400> 38 Val Ala Glu Gin Val Lys Leu Ser Vai 1 Phe Glu Lys Met Tyr Arg Glu Arg Glu 145 Leu Asn Arg Arg Thr 225 Met Thr Ala Pro Giu Ile His Ser Glu 130 Leu Leu Ala His Glu 210 Val1 Ala Pro Leu Asn Val Arg Phe Giu 115 Leu Leu Arg Thr Phe 195 Val1 Phe Thr Pro Val1 Pro Gly Gly Ser 100 Va 1 Phe Asn Lys Glu 180 Ilie Ala Gly Ser 5 Ala Glu Arg His Ile Phe Val1 Met Al a Lys 165 Ser Gly Val1 Asp Pro 245 Asp Phe Thr Thr 70 Ser Ser Val1 His Leu 150 Gin Arg Met Glu Phe 230 Tyr Val Giu Ala Gly 40 Ala Ser Ala Leu Arg Ser Gin Leu Pro Thr 120 Ala Met 135 Glu Giu Ala Arg Ile Val Arg Ala 200 Cys Leu 215 Giu Ile Val Met Trp 25 Arg Asn Leu Ala Ser 105 Leu Asp Lys Gin Val1 185 Ser Arg Giu Asp Glu 10 Ser Ala Ala Glu Thr 90 Gin I le Glu Leu Ala 170 Ser Glu Lys Thr Phe 250 Leu Thr Cys Ala His 75 His Arg Asp Ser G ly 155 Ala Gly His Leu Leu 235 Ala Val1 Giu Leu Asn Leu Val1 Asp 125 Phe Glu Ala Phe Asp 205 Val1 Asp Cys Glu Thr Arg Ala Val1 His 110 Pro Ala Pro Val Arg 190 Val1 Ala Gly Ser Gly Phe His Thr Arg Ser Gln Phe Asn Leu 175 Thr Glu Ala Ser Ser Ala Asp Ile Met His Gly Leu Asn Ala 160 Pro Trp Ile Pro Gln 240 <210> 39 <211> 608 <212> DNA <213> Corynebacteriun glutamicun WO 01/00843 WO 0100843PCT/EBOO/00923 <220> <221> CDS <222> (69)..(608) <223> RXA02843 <400> 39 cccattgcgc ggaggtcgca cccciiccga cttgaaciga taggccgata gaaattattc tggacgicatg act act gct Met Thr Thr Ala gca acc gga at Ala Thr Giy Ile aca ctg acc tcc Thr Leu Thr Ser acc Thr ggc gac gtc ctg Gly Asp Val Leu gig igg tat cca Val Trp Tyr Pro atc ggg tcc acc gac Ile Gly Ser Thr Asp cag tcc gcg cic Gin Ser Ala Leu aca Thr cct cia gaa ggc gic gai gaa gat cga Pro Leu Giu Gly Vai Asp Giu Asp Arg 40 aac gtc Asn Vai acc cgc aaa Thr Arg Lys gac acc tac ASP Thr Tyr gig acg aca act Val Thr Thr. Thr gac acc gac gca Asp Thr Asp Ala gcc ccc acc Ala Pro Thr cac cgc gt His Arg Val gat gca tgg cig cgc cti cac cic cic Asp Ala Trp Leu Arg Leu His Leu Leu tic cgc Phe Arg cct cac acc aic Pro His Thr Ile cta gac ggc at Leu Asp Gly Ile ggc ctc cic aac Giy Leu Leu Asn aat gic gig tgg acc Asn Val Val Trp Thr tic gga ccg tgc Phe Gly Pro Cys git gac ggi tic Val Asp Gly Phe cic acc cgc gcg Leu Thr Arg Ala cgc cig Arg Leu 115 ica cgc cga Ser Arg Arg ggc Giy 120 caa git acg gt Gin Val Thr Val tat agc Tyr Ser 125 gic gac aag Val Asp Lys cgc aic ggi Arg Ile Giy 145 tc Phe 130 cca cgc aig gic Pro Arg Mei Val tai gig git ccc Tyr Vai Val Pro icg ggc gig Ser Gly Val 140 gac gcc gac cgc gic cga cii ggc gcg tac cig gca gat Asp Ala Asp Arg Val Arg Leu Giy Ala Tyr Leu Aia ASP ggc acc Gly Thr 160 acc gig aig cai Thr Val Met His ggc tic gig aac Gly Phe Vai Asn i ic Phe 170 aac gct ggc acg Asn Aia Gly Thr cic Leu 175 ggc gci icc aig Giy Ala Ser Met gti 608 Vali 180 <210> <211> 180 <212> PRT WO 01/00843 WO 0100843PCTA~BOO/00923 <213> Corynebacterium giutamicum <400> Met Thr Thr Ala Ser Ala Thr Giy Ile 1 Asp Ala Lys Tyr Pro Val1 Arg Lys Gly 145 Thr Ala Val1 Leu Ile Asp His Trp Ala Phe 130 Asp Val1 Ser Leu Thr Val Ala Thr Thr Arg 115 Pro Ala Met Met 5 Asp Val Pro Leu Thr Thr Trp Leu Ile Asn Asn Phe 100 Leu Ser Arg Met Asp Arg His Glu 165 Val1 180 Tyr Gly Ile 55 Leu Asp Pro Arg Asp 135 Arg Pro Val1 Asp His Gly Cys Gly 120 Tyr Leu Glu 25 Asp Thr Leu Ile Ala 105 Gin Val1 Gly Ala 10 Ile Glu Asp Leu Phe 90 Val Val Val Ala Thr Gly Asp Ala Ser 75 Gly Asp Thr Pro Tyr 155 Leu Ser Arg Ala His Leu G ly Val Ser 140 Leu Ser Asp Val1 Thr Val1 Asn Ala 110 Ser Val1 Asp Thr Gin Thr Asp Phe Asn Leu Val1 Arg Gly Gly Ser Arg Thr Arg Val1 Thr Asp Ile Thr 160 Gly Phe Val Asn Phe Asn Ala Gly Thr Leu Gly 170 175 <210> 41 <211> 1143 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(1120) <223> RXN00355 <400> 41 aatagatcag cgcatccgtg gtggaaccaa aaggctcaac aatacgaaac gttcgctttc ggtcctgatg aaagagatgt ccctgaatca tcatctaagt atg cat ctc ggt aag 115 Met His Leu Gly Lys 1 ctc gac cag gac agt gcc acc aca att ttg gag gat tac aag aac atg 163 Leu Asp Gin Asp Ser Ala Thr Thr Ile Leu Glu Asp Tyr Lys Asn Met 15 WO 01/00843 ac c Thr gtc Val1 t tc Phe gcc Ala atg Met ttc Phe cac His ctg Leu tac Tyr 150 cca Pro gt t Val1 aag Lys aag Lys gaa Giu 230 gaa Giu aac Asn gaa Giu tcg Ser gac Asp ggC Gly gcc Ala cgc Arg gtc Val1 135 gca Ala gg t Gly caa Gin gcc Ala cgc Arg 215 aac Asn gtc Val1 at c Ile aag Lys cgc Arg gtg Va 1 tcc Ser tgc Cys cag Gin 120 tct Ser gcg Ala ttg Leu aag Lys cgc Arg 200 c aa Gin gac Asp aac Asn cgc Arg ctt Leu Cgg Arg gac Asp gcc Ala acc Thr 105 gtc Val1 acc Thr gca Ala tca Ser gca Aia 185 cgc Arg tgc Cys atc Ile t tc Phe gta Val1 att Ilie gcc Ala aag Lys acc Thr gta Val1 atg Met ggC Giy gtc Vali cag Gin 170 gtc Val1 ggc Gly ttc Phe cgc Arg atc Ile 250 gc t Ala gcc Ala acc Thr cac His 75 gac Asp gac Asp aac Asn tgg Trp tta Leu 155 ggC Gly cag Gin gaa Glu gtg Val acc Thr 235 gac Asp gtg Val1 cag Gin gac Asp gac Asp cc t Pro tac Tyr gcc Ala 125 cca Pro gag Glu tcc Ser acc Thr ggc Gly 205 gcc Aia cc t Pro gca Ala ggc Gly ccc Pro aca Thr gac Asp gag Giu gac Asp 110 gcc Ala gga Gly cac His gat Asp ctc Leu 190 gac Asp gac Asp gat Asp acc Thr tac Tyr gac Asp aag Lys gtg Val cag Gin 95 aac Asn acc Thr atg Met cag Gin gc t Ala 175 cca Pro ctt Leu gcg Ala tac Tyr ttc Phe 255 acc gga Giy atg Met acg Thr gac Asp 80 gca Ala cac His gca Aia t tc Phe cag Gin 160 ttg Leu tcc Ser acc Thr gcc Ala ttc Phe 240 gac Asp ggc aac Asn gac Asp cca Pro gtg Val1 cca Pro cgc Arg gcc Ala tcc Ser 145 cac His cga Arg gaa Giu gga Gly gat Asp 225 gtt Val1 tcc Ser gac PCTIBOOIOO923 cgc agc 211 Arg Ser gga atc 259 Gly Ile gat gtc 307 Asp Vai ctg tgc 355 Leu Cys gcg cag 403 Aia Gin 100 cca cgc 451 Pro Arg gtt gca 499 Val Aia cgc gtc 547 Arg Val tgg ggc 595 Trp Gly 165 cct ggc 643 Pro Giy 180 ctg gaa 691 Leu Glu acc cac 739 Thr His cgc atc 787 Arg Ile gaa gtc 835 Glu Vai 245 acc ggc 883 Thr Gly 260 ggc ttc 931 atg cca cac ggt ggc cac gtg att acc 57 WO 01/00843 Met Pro His Gly Gly His Val Ile Thr 265 270 aac cac acc gtg gaa tac atc ctc aag Asn His Thr Val Giu Tyr Ile Leu Lys 280 285 acc gct tcc tca cag atc gct ttc ggt 1027 Thr Ala Ser Ser Gin Ile Ala Phe Gly 295 300 cag cag ggc caa agc gga gct ttc acc 1075 Gin Gin Gly Gin Ser Gly Aia Phe Thr 310 315 ctg ctc tcc cca gag aac ttg gac gat 1120 Leu Leu Ser Pro Giu Asn Leu Asp Asp 330 taatttagct cgaggggcaa gga 1143 <210> 42 <2i1> 340 <212> PRT <2i3> Corynebacteriun glutarnicum <400> 42 Met His Leu Giy Lys Leu Asp Gin Asp Thr Gly ctg gac Leu Asp cgc gca Arg Aia gtc ctc Val Leu 320 ctg atc Leu Ile 335 Ser Ala 10 Val Ala Ile Ala Ala Thr Lys His 75 Thr Asp Val Asp Met Asn Gly Trp, Val Leu 155 Asp Thr cga aac Arg Asn 290 gct cac Ala His 305 gaa gtt Giu Val gca cgc Ala Arg Thr Thr Ile Val Lys Gin Leu Asp Ala Asp Ile Pro Thr Tyr Glu Ala 125 Asp Pro 140 Ala Giu Gly 275 c ca Pro cgc Arg gct Ala gac Asp Ile Gly Pro Thr Asp Glu Asp 110 Ala Gly His PCTIIBOO/00923 Gly Phe gat ttc 979 ASP Phe atg aag Met Lys cca tac Pro Tyr 325 gtc Val1 340 1 Asp Asn Asp Pro Val1 Pro Arg Ala Ser 145 Tyr Leu Leu Val Leu Lys Asp Gly 130 Ile Thr Val Phe Ala 70 Met Phe His Leu Tyr 150 Asn Glu Ser 55 Asp Gly Ala Arg Val1 135 Ala Ile Lys Arg Val1 Ser Cys Gin 120 Ser Ala Leu Tyr Asp Lys Val1 Gin Asn Thr Met Gin Glu Gly Met Thr Asp Ala His Ala Phe Gin 160 WO 01/00843 WO 0100843PCT/iBOO/00923 His Thr Phe Trp Gly Pro Gly Leu Ser Gin Gly His Ser Asp Ala Leu 165 170 175 Arg Arg Ile Pro Giy Vai Gin Lys Ala Val Gin Tyr Thr Leu Pro Ser 180 185 190 Giu Asp Ala Leu Giu Lys Ala Arg Arg Giy Giu Ala Gly Asp Leu Thr 195 200 205 Giy Lys Gin Thr His Lys Arg Gli Cys Phe Val Val Ala Asp Ala Ala 210 215 220 Asp His Giu Arg Ilie Giu Asn Asp Ile Arg Thr Met Pro Asp Tyr Phe 225 230 235 240 Val Giy Tyr Giu Val Giu Val Asn Phe Ile Asp Giu Ala Thr Phe Asp 245 250 255 Ser Giu His Thr Gly Met Pro His Gly Gly His*Val Ile Thr Thr Gly 260 265 270 Asp Thr Gly Gly Phe Asn His Thr Val Glu Tyr Ile Leu Lys Leu Asp 275 280 285 Arg Asn Pro Asp Phe Thr Ala Ser Ser Gin Ile Ala Phe Gly Arg Ala 290 295 300 Ala His Arg Met Lys Gin Gin Gly Gin Ser Gly Ala Phe Thr Val Leu 305 310 315 320 Giu Val Ala Pro Tyr Leu Leu Ser Pro Giu Asn Leu Asp Asp Leu Ile 325 330 335 Ala Arg Asp Val 340 <210> 43 <211> 958 <212> DNA <213> Corynebacterium giutariicum <220> <221> CDS <222> (101)..(958) <223> FRXA00352 <400> 43 aatagatcag cgcatccgtg gtggaaccaa aaggctcaac aatacgaaac gttcgctttc ggtcctgatg aaagagatgt ccc tgaatca tcatctaagt atg cat ctc ggt aag 115 Met His Leu Gly Lys 1 ctc gac cag gac agt gcc acc aca att ttg gag gat tac aag aac atg 163 Leu Asp Gin Asp Ser Ala Thr Thr Ile Leu Giu Asp Tyr Lys Asn Met 15 acc aac atc cgc gta gct atc gtg ggc tac gga aac ctg gga cgc agc 211 Thr Asn Ilie Arg Val Ala Ile Val Gly Tyr Gly Asn Leu Gly Arg Ser 59 WO 01/00843 WO 0100843PCT/EBOO/00923 gta gga atc Val Gly Ile gtc gaa aag Val Giu Lys ctt aft gcc aag cag ccc gac atg gac Leu Ile Ala Lys Gin Pro ASP Met Asp ctt Leu ttc tcg cgc cgg gcc acc Phe Ser Arg Arg Ala Thr gac aca aag acg Asp Thr Lys Thr gtc ttt gat gtc Vai Phe Asp Val gcc Ala gac gtg gac aag cac gcc gac gac gtg Asp Val Asp Lys His Ala Asp Asp Val gtg ctg ttc ctg Val Leu Phe Leu atg ggc tcc gcc acc gac atc cct gag Met Gly Ser Ala Thr Asp Ile Pro Giu gca cca aag ttc Ala Pro Lys Phe gcg cag Ala Gin 100 ttc gcc tgc Phe Ala Cys cac cgc cag His Arg Gin 120 gta gac acc tac Val Asp Thr Tyr aac cac cgc gac atc cca cgc Asn His Arg Asp Ile Pro Arg 115 gtc atg aac gaa Val Met Asn Glu gcc acc gca gcc Ala Thr Ala Ala ggc G ly 130 aac gtt gca Asn Val Ala ctg gtc Leu Vai 135 tct acc ggc tgg Ser Thr Gly Trp, cca gga atg ttc Pro Gly Met Phe tcc Ser 145 atc aac cgc gtc Ile Asn Arg Vai gca gcg gca gtc Ala Ala Ala Val gcc gag cac cag Ala Giu His Gin cac acc ttc tgg His Thr Phe Trp cca ggt ttg tca Pro Giy Leu Ser cag Gin 170 ggc cac tcc gat Gly His Ser Asp ttg cga cgc atc Leu Arg Arg Ile cct ggc Pro Gly 180 gtt caa aag Vai Gin Lys aag gcc cgc Lys Ala Arg 200 gca Aia 185 gtc cag tac acc Val Gin Tyr Thr cca tcc gaa gac Pro Ser Giu Asp gcc ctg gaa Ala Leu Giu 195 caa acc cac Gin Thr His cgc ggc gaa gcc Arg Gly Giu Ala gac ctt acc gga Asp Leu Thr Gly aag Lys 210 aag cgc Lys Arg 215 caa tgc ttc gtg Gin Cys Phe Val gcc gac gcg gcc Ala Asp Ala Ala gat

ASP

225 cac gag cgc atc His Giu Arg Ile gaa Giu 230 aac gac atc cgc Asn Asp Ile Arg atg cct gat tac Met Pro Asp Tyr gtt ggc tac gaa Val Gly Tyr Giu gaa gtc aac ttc Giu Val Asn Phe gac gaa gca acc Asp GiU Ala Thr gac tcc gag cac Asp Ser Giu His acc ggc Thr Gly 260 atg cca cac Met Pro His gg t Gly 265 ggc cac gtg att Gly His Vai Ile acc ggc gac acc Thr Gly Asp Thr ggt ggc ttc Giy Gly Phe 275 WO 01/00843 PCT/IB00/00923 aac cac acc gtg gaa tac ate etc aag 958 Asn His Thr Val Glu Tyr Ile Leu Lys 280 285 <210> 44 <211> 286 <212> PRT <213> Corynebacterium glutamicum <400> 44 Met His Leu Gly Lys Leu Asp Gin Asp Ser Ala Thr Thr Ile Leu Glu 1 5 10 Asp Tyr Lys Asn Met Thr Asn Ile Arg Val Ala Ile Val Gly Tyr Gly 25 Asn Leu Gly Arg Ser Val Glu Lys Leu Ile Ala Lys Gin Pro Asp Met 40 Asp Leu Val Gly Ile Phe Ser Arg Arg Ala Thr Leu Asp Thr Lys Thr 55 Pro Val Phe Asp Val Ala Asp Val Asp Lys His Ala Asp Asp Val Asp 70 75 Val Leu Phe Leu Cys Met Gly Ser Ala Thr Asp Ile Pro Glu Gin Ala 90 Pro Lys Phe Ala Gin Phe Ala Cys Thr Val Asp Thr Tyr Asp Asn His 100 105 110 Arg Asp Ile Pro Arg His Arg Gin Val Met Asn Glu Ala Ala Thr Ala 115 120 125 Ala Gly Asn Val Ala Leu Val Ser Thr Gly Trp Asp Pro Gly Met Phe 130 135 140 Ser Ile Asn Arg Val Tyr Ala Ala Ala Val Leu Ala Glu His Gin Gin 145 150 155 160 His Thr Phe Trp Gly Pro Gly Leu Ser Gin Gly His Ser Asp Ala Leu 165 170 175 Arg Arg Ile Pro Gly Val Gin Lys Ala Val Gin Tyr Thr Leu Pro Ser 180 185 190 Glu Asp Ala Leu Glu Lys Ala Arg Arg Gly Glu Ala Gly Asp Leu Thr 195 200 205 Gly Lys Gin Thr His Lys Arg Gin Cys Phe Val Val Ala Asp Ala Ala 210 215 220 Asp His Glu Arg Ile Glu Asn Asp Ile Arg Thr Met Pro Asp Tyr Phe 225 230 235 240 Val Gly Tyr Glu Val Glu Val Asn Phe Ile Asp Glu Ala Thr Phe Asp 245 250 255 Ser Glu His Thr Gly Met Pro His Gly Gly His Val Ile Thr Thr Gly 61 WO 01/00843 WO 0100843PCT/EBO0100923 Asp Thr Gly Gly Phe Asn His Thr Val Giu Tyr Ile Leu Lys 275 280 285 <210> <211> 1400 <212> DNA <213> Corynebacterium glutamicum <22 0> <221> CDS <222> (1)..(1377) <223> RXA00972 <400> cct gca cct ggt tgg cgt ttc cgc acc gga gaa gat gta aca atg gct Pro Ala Pro Gly Trp Arg Phe Arg Thr Gly Giu Asp Val Thr Met Ala 1 5 10 aca gtt gaa Thr Val Giu gcc gtg cgc Ala Val Arg aat Asn ttc aat gaa ctt Phe Asn Giu Leu gca cac gta tgg Ala His Val Trp cca cgc aat Pro Arg Asn gtg cct ctg Val Pro Leu caa gaa gac ggc Gin Giu Asp Gly gtc acc gtc gct Val Thr Val Ala cct gac Pro Asp ctc gct gaa gaa Leu Ala Glu Glu gga acc cca ctg ttc gta gtc gac gag Gly Thr Pro Leu Phe Val Val Asp Giu gac Asp gat ttc cgt tcc Asp Phe Arg Ser tgt cgc gac atg Cys Arg Asp Met acc gca ttc ggt Thr Ala Phe Gly cca ggc aat gtg Pro Gly Asn Val tac gca tct aaa Tyr Ala Ser Lys gcg Ala ttc ctg acc aag Phe Leu Thr Lys acc att Thr Ile gca cgt tgg Ala Arg Trp gat gaa gag ggg Asp Giu Glu Gly ctg gca ctg gac att gca tcc atc Leu Ala Leu Asp Ile Ala Ser Ile 105 110 gct ggt ttc ccc gcc agc cgt atc Ala Gly Phe Pro Ala Ser Arg Ile aac gaa ctg ggc att gcc ctg Asn Giu Leu Gly Ilie Ala Leu acc gcg Thr Ala 130 cac ggc aac aac His Gly Asn Asn ggc gta gag ttc ctg cgc gcg ttg gtt Gly Val Giu Phe Leu Arg Ala Leu Val 384 432 480 caa aac ggt gtg gga Gin Asn Gly Val Gly 145 gtg gtg ctg gac tcc gca cag gaa cta Val Val Leu ASP Ser Ala Gin Giu Leu 155 gaa Giu 160 ctg ttg gat tac Leu Leu Asp Tyr gcc gct ggt gaa Ala Ala Gly Giu aag att cag gac Lys Ile Gin Asp gtg ttg Val Leu 175 WO 01/00843 atc cgc Ile Arg act agc Thr Ser gca ttc Ala Phe 210 gtt ggc Val Gly 225 ttc aag Phe Lys agc gaa Ser Glu ggc att Gly Ile gcc tcc Ala Ser 290 atc gac Ile Asp 305 ccc tcc 1008 Pro Ser gta gac 1056 Val Asp tcc gac 1104 Ser Asp gta gta 1152 Val Val 370 ggc tcc 1200 Giy Ser 385 gta Val cac His 195 gaa Giu ctg Leu ctg Leu ctg Leu gcc Ala 275 gac Asp gca Ala acc Thr gac Asp aac Asn 355 tcc Ser cac His aag Lys 180 gaa Giu gca Ala cac His gca Ala ggc Gly 260 tat Tyr c tg Leu cca Pro gtg Val1 gac Asp 340 atc Ile cgc Arg tgc Cys cca Pro gac Asp gca Ala tgc Cys gca Ala 245 gtt Val acc Thr ctc Leu acc Thr acc Thr 325 aaa Lys cgc Arg ttc Phe gaa Glu ggc Gly cag Gin aaa Lys cac His 230 gaa Giu gcc Ala gca Ala acc Thr gtg Val1 310 atc Ile acc Thr cca Pro gcc Ala tcc Ser 390 atc Ile aag Lys gcc Ala 215 gtt Val1 cgc Arg ctt Leu gc t Ala gca Ala 295 ctt Leu tac Tyr cgc Arg gca Ala gaa Giu 375 ggC Gly gaa Giu t tc Phe 200 gcc Ala ggt Gly gtg Val cc t Pro gaa Glu 280 gtc Val1 gtt Val gaa Glu cgt Arg ctc Leu 360 gga G ly gat Asp gca Ala 185 gga Gly aac Asn tcc Ser t tg Leu gaa Giu 265 gaa Giu gga Gly gag Giu gtc Val1 tac Tyr 345 t ac Tyr gac

ASP

atc Ile cac His t tc Phe aac Asn cag Gin Gly 250 ctg Leu cca Pro aaa Lys ccc Pro ggc Gly 330 atc Ile ggg Gly cca Pro ctg acc Thr tcc Ser gca Ala gtg Val 235 ctg Leu gat Asp C tC Leu atg Met ggc Gly 315 acc Thr gcc Ala tcc Ser gta Val1 atc cac His ctg Leu gaa Giu 220 t tc Phe tac Tyr ctc Leu aac Asn gca Ala 300 cgc Arg acc Thr gtg Val gaa Giu agc Ser 380 aac gag Giu gca Ala 205 aac Asn gac Asp tca Ser ggt Gly gtc Val 285 gcg Ala gc t Ala aaa Lys gac Asp tac Tyr 365 acc Thr gat ttc Phe 190 t cc Ser ctg Leu gcc Ala cag Gin Gly 270 gca Ala gaa Giu atc Ile gac Asp gga G ly 350 gac Asp cgc Arg gaa PCT/IBOO/00923 atc gcc 576 Ile Ala ggt tcc 624 Gly Ser aac ctg 672 Asn Leu gaa ggc 720 Giu Gly .240 atc cac 768 Ile His 255 gga tac 816 Gly Tyr gaa gtt 864 Giu Val cta ggc 912 Leu Gly gca ggc 960 Ala Gly 320 gtc cac Val His 335 ggc atg Gly Met gcc cgc Ala Arg atc gtg Ile Val atc tac Leu Ile 395 Asn Asp Giu Ile Tyr 400 WO 01/00843 WO 0100843PCTAIB00/00923 cca tct gac atc acc agc ggc gac ttc 1248 Pro Ser Asp Ile Thr Ser Gly Asp Phe 405 gca tac tgc tac gcc atg agc tcc cgc 1296 Ala Tyr Cys Tyr Ala Met Ser Ser Arg 420 425 gcc gtc gtg tcc gtc cgc gct ggc agc 1344 Ala Val Val Ser Val Arg Ala Gly Ser 435 440 gaa acg ctc gac gac atc ctc tca cta 1397 Glu Thr Leu ASP Asp Ile Leu Ser Leu 450 455 ccc 1400 <210> 46 <211> 459 <212> PRT <213> Corynebacteriun glutamicum Ct t Leu 410 tac TIyr tcc Ser gag Giu ctc gca gcc acc ggc Leu Ala Ala Thr Gly 415 gcc ttc aca cgg ccc Ala Phe Thr Arg Pro 430 ctc atg ctg cgc cgc Leu Met Leu Arg Arg 445 taacgctttt cgacgcctga <400> 46 Pro Ala Pro Gly Trp Arg Phe Arg Thr Gly Giu Asp Val Thr Met Ala 1 Thr Ala Pro Asp Pro Ala Asn Thr Gin 145 Giu Arg Leu Phe Asn Trp Leu 115 His Gly 5 Phe Giu Glu Ser His Asp Ile Asn Gly Asn Asp Giu Arg 70 Tyr Giu Ala Asn His 150 Giu Gly Tyr Cys Ala Glu Leu Lys 135 Val1 Leu Val1 40 Gly Arg Ser Gly Ala 120 Gly Val1 Pro Val Thr Asp Lys Leu 105 Ala Val1 Leu 10 Ala Thr Pro Met Ala 90 Ala Gly Glu Asp Gly His Val1 Leu Ala 75 Phe Leu Phe Phe Ser 155 Lys Val Ala Phe Thr Leu Asp Pro Leu 140 Ala Ile Trp Gly Val1 Ala Thr Ile Ala 125 Arg Gin Gin Pro Val Val1 Phe Lys Ala 110 Ser Ala Giu

ASP

Arg Pro Asp Gly Thr Ser Arg Leu Leu Val1 Asn Leu Giu Gly Ile Ile Ile Val Giu 160 Leu Leu Leu Asp Tyr Val Ala Ala Gly Giu WO 01/00843 WO 0100843PCT/IBOO/00923 165 170 175 Ilie Arg Val Lys Pro Gly Ilie Giu Ala His Thr His Glu Phe Ile Ala 180 185 190 Thr Ser His Giu Asp Gin Lys Phe Gly Phe Ser Leu Ala Ser Gly Ser 195 200 205 Ala Phe Glu Ala Ala Lys Ala Ala Asn Asn Ala Glu Asn Leu Asn Leu 210 215 220 Val Giy Leu His Cys His Val Gly Ser Gin Vai Phe Asp Ala Glu Gly 225 230 235 240 Phe Lys Leu Ala Ala Glu Arg Val Leu Gly Leu Tyr Ser Gin Ilie His 245 250 255 Ser Glu Leu Giy Val Ala Leu Pro Glu Leu Asp Leu Giy Gly Gly Tyr 260 265 270 Gly Ile Ala Tyr Thr Ala Ala Glu Giu Pro Leu Asn Val Ala Glu Val 275 280 285 Ala Ser Asp Leu Leu Thr Ala Val Gly Lys Met Ala Ala Giu Leu Gly 290 295 300 Ilie Asp Ala Pro Thr Val Leu Val Giu Pro Gly Arg Ala Ile Ala Gly 305 310 315 320 Pro Ser Thr Val Thr Ile Tyr Glu Val Gly Thr Thr Lys Asp Val His 325 330 335 Val Asp Asp Asp Lys Thr Arg Arg Tyr Ile Ala Val Asp Gly Gly Met 340 345 350 Ser Asp Asn Ile Arg Pro Ala Leu Tyr Gly Ser Glu Tyr Asp Ala Arg 355 360 365 Val Val Ser Arg Phe Ala Glu Gly Asp Pro Val Ser Thr Arg Ile Val 370 375 380 Gly Ser His Cys Giu Ser Gly Asp Ile Leu Ile Asn Asp Glu Ile Tyr 385 390 395 400 Pro Ser Asp Ilie Thr Ser Gly Asp Phe Leu Ala Leu Ala Ala Thr Gly 405 410 415 Ala Tyr Cys Tyr Ala Met Ser Ser Arg Tyr Asn Ala Phe Thr Arg Pro 420 425 430 Ala Val Val Ser Val Arg Ala Gly Ser Ser Arg Leu Met Leu Arg Arg 435 440 445 Giu Thr Leu Asp Asp Ile Leu Ser Leu Glu Ala 450 455 <210> 47 <211> 2121 <212> DNA <213> Corynebacterium glutamicum WO 01/00843 WO 0100843PCTIIBOO/00923 <220> <221> CDS <222> (2098) <223> RXA02653 <400> 47 agacagagtg ttagtgcgtg gggcagctct cactttcatc accggccgta ttcattccaa taacccgcac agggaaacta gacatcactc gagtatgctc atg ata ccg aag ccc ii! Met Ile Pro Lys Pro gac gtg acc gac tta tat tta gag gac Asp Val Thr Asp Leu Tyr Leu Glu Asp tta aat gag ggt Leu Asn Giu Gly tcg gaa Ser Giu aag att cgg Lays Ilie Arg gag gtt tcc Giu Val Ser tcc Ser gcc aag gat ctt Ala Lys Asp Leu gaa ctt agg aca Giu Leu Arg Thr gtt cta aaa Val Leu Lys gat gaa gaa Asp Giu Giu tcc caa att cag gaa cga gct ggg aaa Ser Gin Ile Gin Giu Arg Ala Gly Lys tgg gga Trp Giy atg ggg gcc act Met Gly Ala Thr cgg gag ctg tac Arg Glu Leu Tyr agc atc gtg gaa Ser Ile Val Giu gct tcc tac gaa ggg cgt gac agc cta Aia Ser Tyr Giu Giy Arg Asp Ser Leu gga ttt gat cac Gly Phe Asp His gcc cgg gaa atg Ala Arg Glu Met aga tta gcc ttc Arg Leu Aia Phe cca cca tcc gaa Pro Pro Ser Giu agt ttt Ser Phe 100 gaa tac ctc Giu Tyr Leu ctg cat cgt Leu His Arg 120 caa Gin 105 gaa ctc gta aaa Giu Leu Vai Lys gga gtg gta gac Giy Val Val Asp atc act cac Ile Thr His 115 gaa ctt gaa Giu Leu Giu ggc cgg gaa cca ctg aca gat tta gtt Giy Arg Giu Pro Leu Thr Asp Leu Val 125 ata act Ile Thr 135 gtg gtg ata gac gct gtt ctt ccc ccg Val Vai Ile Asp Ala Val Leu Pro Pro 140 gga gta gtg cca Gly Val Val Pro ggc G ly 150 aca ttg gtg cac Thr Leu Val His aa t Asn 155 ttg gta aaa gag Leu Val Lys Giu tat gcc aga atg Tyr Ala Arg Met cct ggg act cgg Pro Gly Thr Arg tta gat gta gcg Leu Asp Val Ala gac ggc acc gtt Asp Gly Thr Val caa ggg Gin Gly 180 caa cga cat Gin Arg His t tg Leu 185 gct gca gtc gga Ala Ala Val Gly atg acg gaa gat Met Thr Giu Asp gtg gtt ttg Val Val Leu 195 WO 01/00843 PCTIBOO/00923 ,ac tqq 739 ggt aat Gly Asn gct cgt Ala Arg 215 ggt act Gly Thr 230 act tca Thr Ser cct gtg Pro Val ttg gtt Leu Val gcc cgc 1027 Ala Arg 295 acc ggt 1075 Thr Gly 310 ctt aat 1123 Leu Asn aaa ccg 1171 Lys Pro tct gtg 1219 Ser Val gac cgc 1267 Asp Arg 375 tta cct 1315 Leu Pro 390 ctt acc 1363 gac Asp 200 cga Arg cca Pro gat Asp aa t Asn gac Asp 280 aaa Lys cat His cgt Arg gac Asp gat Asp 360 gtt Val1 cca Pro gag ac a Thr gtt Val1 ccg Pro ccg Pro gta Val1 265 gcc Ala gcg Ala ggt Gly gga Gly aga Arg 345 tcg Ser gca Ala act Thr gtg ttg Leu atc Ile ctt Leu gcc Ala 250 ctc Leu ggc Gly aat Asn gta Val1 gtc Val1 330 cta Leu cgt Arg cga Arg aga Arg ata tcg Ser cgc Arg ccg Pro 235 aca Thr cat His att Ile aag Lys gat Asp 315 c ca Pro t tg Leu gat Asp gtt Val ttt Phe 395 ccc cga Arg gac Asp 220 gca Ala tgc Cys tca Ser cag Gin ggt Gly 300 gta Val1 gga Gly gca Ala gaa Glu gcg Ala 380 gg t Gly ggC tca Ser 205 gcg Ala cgg Arg cgc Arg ggt Gly atg Met 285 ctt Leu gcc Ala gag Glu t ta Leu t ta Leu 365 cc t Pro gag Glu gtg gac Asp tat Tyr ccc Pro 240 att Ile cct Pro gat Asp gtt Val1 cga Arg 320 att Ile gaa Glu att Ile gct Ala gca Ala 400 gtg ata Ile ccc Pro 225 tgg Trp, gaa Giu cgt Arg act Thr gat Asp 305 gag Giu cta Leu aat Asn tcg Ser cca Pro 385 gac Asp gg t atc Ile 210 gat Asp gcg Ala gaa Glu aat Asn cga Arg 290 gcc Ala tta Leu tcc Ser ggc Gly gct Ala 370 gat Asp tgg Trp ctt ccg Pro agg Arg gaa Giu t tc Phe ata Ile 275 ata Ile gtt Val1 tct Ser gca Ala gtg Val1 355 t tg Leu cc t Pro gg t Gly cac WO 01/00843 WO 0100843PCT/IBOO/00923 Leu Thr Giu Val ctc cat 1411 Leu His tgc caa 1459 Cys Gin att gac 1507 Ile Asp 455 gat tgg 1555 Asp Trp 470 gcc gaa 1603 Ala Giu ttc tat 1651 Phe Tyr aag ggg 1699 Lys Gly gag cct 1747 Giu Pro 535 gtt gct 1795 Val Ala 550 gct atg 1843 Ala met gat ccc 1891 Asp Pro tat cta 1939 Tyr Leu ggc tat Gly Tyr 425 ctc gtc Leu Val Ile 410 gc t Ala gat Asp Pro Gly Val gca aaa gac Ala Lys Asp tct ctc aga Ser Leu Arg Asp cgt Arg 430 Ile 415 gct Ala Val Gly Leu His Val His 420 ctg gct ctg cag gaa tgt Leu Ala Leu Gin Glu Cys 435 440 ctt Leu 445 gaa tgc ggg cat tcc cca cag ttt Giu Cys Gly His Ser Pro Gin Phe 450 atg agc tac att gaa tct gag gaa Met Ser Tyr Ile Giu Ser Giu Giu gga gga ggg gtg Gly Gly Gly Val 460 cct Pro atc cgt tat caa Ile Arg Tyr Gin 475 tcc ttt acg tgg Ser Phe Thr Trp tcc gct aaa tct Ser Ala Lys Ser aaa gac gat ccg Lys Asp Asp Pro gcg Ala 480 tca gcc ggg tat Ser Ala Gly Tyr 485 cag acc Gin Thr 505 490 cca Pro 495 gtg cgc ggt aat tgg Val Arg Gly Asn TrP 510 atg ctc att gac cgg Met Leu Ile Asp Arg tta tct aat acg tac ccg Leu Ser Asn Thr Tyr Pro 500 ttg aaa gac gtg ctt tct Leu Lys Asp Val Leu Ser 515 gga ttg cgg tta cac ata.

Giv Leu Arg Leu His Ile gta gct cag Val Ala Gin 520 ggt cga agt Giy Arg Ser 525 tta cta gat Leu Leu Asp ttt gtg aaa acc Phe Val Lys Thr 555 aac cga acg cag Asn Arg Thr Gin 570 540 cga Arg agt Ser ggg tgt ggc gtc Gly Cys Gly Val 545 gac ggg ttg cct Asp Giv Leu Pro 530 act ctt gcc gaa Thr Leu Ala Giu cta gtg gga ctg Leu Val Gly Leu 565 gat ttt ctc att Asp Phe Leu Ilie 560 tgc cgg act aca Cys Arg Thr Thr 575 tcc gat Ser Asp 580 ctg cat Leu His 585 gtg ggt Val Gly atc act gac ggt gat gta ggc gag gaa atc gaa gca Ilie Thr Asp Gly Asp Val Gly Giu Giu Ile Giu Ala 590 595 gcc tac tgc atc gaa gat gag ctg att tta cgc cgg Ala Tyr Cys Ile Giu Asp Giu Leu Ile Leu Arg Arg WO 01/00843 WO 0100843PCT/IBOO/00923 600 605 610 cga atc cgc ttc ccg aga gga gtc aaa cca gga gat atc atc gga att 1987 Arg Ilie Arg Phe Pro Arg Giy Val Lys Pro Gly Asp Ile Ile Gly Ile 615 620 625 cct aac acc gca gga tac ttc atg cat atc ttg gaa agt gca tcg cac 2035 Pro Asn Thr Ala Gly Tyr Phe Met His Ile Leu Giu Ser Ala Ser His 630 635 640 645 caa atc ccg ttg gcg aaa aat gta gtg tgg ccg gag ggg cag tta gac 2083 Gin Ilie Pro Leu Ala Lys Asn Val Val Trp Pro Giu Gly Gin Leu Asp 650 655 660 gat atc gat gcg gat taagacataa ccattcgcta atc 2121 Asp Ilie Asp Ala Asp 665 <210> 48 <211> 666 <212> PRT <213> Corynebacteriun giutanicun <400> 48 Met Ilie Pro Lys Pro Asp Val Thr Asp Leu Tyr Leu Giu Asp Leu Leu 1 5 10 Asn Giu Giy Ser Giu Lys Ilie Arg Ser Ala Lys Asp Leu Ser Giu Leu 25 Arg Thr Vai Leu Lys Giu Vai Ser Ser Gin Ile Gin Giu Arg Ala Giy 40 Lys Lys Asp Giu Giu Trp Gly Met Giy Ala Thr Trp Arg Giu Leu Tyr 55 Pro Ser Ile Val Giu Arg Ala Ser Tyr Giu Giy Arg Asp Ser Leu Ile 70 75 Gly Phe Asp His Leu Ala Arg Giu Met Giu Arg Leu Ala Phe Giy Pro 90 Pro Ser Giu Ser Phe Giu Tyr Leu Gin Giu Leu Val Lys Ser Giy Val 100 105 110 Vai Asp Ile Thr His Leu His Arg Giy Arg Giu Pro Leu Thr Asp Leu 115 120 125 Vai Arg Giu Leu Giu Ile Thr Val Val Ile Asp Ala Val Leu Pro Pro 130 135 140 Pro Gly Val Val Pro Giy Thr Leu Val His Asn Leu Val Lys Giu Giy 145 150 155 160 Tyr Ala Arg Met Arg Pro Gly Thr Arg Gly Leu Asp Val Ala Ala Asp 165 170 175 WO 01/00843 WO 0100843PCTIBOO/00923 Gly Glu Ile Pro 225 Trp, Glu Arg Thr

ASP

305 Glu Leu Asn S er Pro 385 Asp G ly Ala His Ile 465 Arg Asn Arg 215 Thr Ser Val Val Arg 295 Gly Asn Pro Val1 Arg 375 Pro Thr His Gin Asp 455 Trp, His Asp 200 Arg Pro Asp Asn Asp 280 Lys His Arg Asp Asp 360 Val1 Pro Giu Gly Leu 440 Leu Ile Leu 185 Thr Val Pro Pro Val 265 Ala Ala Gly Gly Arg 345 Ser Ala Thr Val1 Tyr 425 Val1 Gly Arg Ala Leu Ile Leu Ala 250 Leu Gly Asn Val Val1 330 Leu Arg Arg Arg Ile 410 Ala Asp Gly Tyr Thr 490 Ala Ser Arg Pro 235 Thr His Ile Lys Asp 315 Pro Leu Asp Val1 Phe 395 Pro Ala Ser Giy Gin 475 Trp Arg 190 Leu Ser Leu His Ser 270 Gly Thr Ser Arg Ala 350 Asp Arg Arg Asp Arg 430 Giu Met Lys Asp Met His Thr Glu Leu 255 Met Val1 Phe Glu Ile 335 Ile Arg Val1 Ala Ile 415 Ala Cys Ser Ser Pro 495 Thr Ser Ala Gly Ala Glu Ser Phe WO 01/00843 WO 0100843PCTJIBOO/00923 Ser Lys Leu Val1 545 Pro Asp Giu Leu Asp 625 Giu Asn Asp Arg 530 Thr Leu Asp Giu Ile 610 Ile Ser Thr Val 515 Leu Leu Val1 Phe Ile 595 Leu Ile Ala Tyr 500 Leu His Ala Gly Leu 580 Giu Arg Gly Ser Leu 660 Pro Ser Ile Giu Leu 565 Ile Ala Arg Ilie His 645 Gin Val1 520 Gly Phe Asn Leu Val1 600 Arg Thr Pro Pro Gin Ser Lys Thr 570 Ile Aia Pro Gly Ala 650 Val Met Leu Thr 555 Gin Thr Tyr Arg Tyr 635

LYS

Arg Leu Leu 540 Arg Cys Asp Cys Gly 620 Phe Asn Giy Ile 525 Asp Ser Arg Gly Ile 605 Vali Met Val1 Asn 510 Asp Gly Asp Thr Asp 590 Giu Lys His Val Giu Gly Gin Asp Asp Ile Asp Ala Asp <210> 49 <2ii> 993 <212> DNA <213> Corynebacterium giutainicum <22 0> <221> CDS <222> (101)..(970) <223> RXA01393 <400> 49 caaaagcaga cctgtaatga agatttccat gatcaccatc agtaaaatga ttggttctta acatggttta atatagcttc ctg gac act ttg ctc tca atc att gat gaa ggc Leu Asp Thr Leu Leu Ser Ile Ile Asp Giu Gly i5 tcc tta gcc ctt tcc att tcc ccc tcg gcg gtg Ser Leu Ala Leu Ser Ile Ser Pro Ser Aia Val 30 gct ctc gag cat cac gtg ggt cga gtg ttg gta Ala Leu Giu His His Vai Gly Arg Val Leu Vai 71 gtgacctatg gaagtactta atg aac ccc att caa 115 Met Asn Pro Ile Gin 1 agc ttc gaa ggc gcc 163 Ser Phe Giu Giy Ala agt cag cgc gtt aaa 211 Ser Gin Arg Val Lys tcg cgc acc ca a ccg 259 Ser Arg Thr Gin Pro WO 01/00843 WO 0100843PCT/IBOO/00923 gcc aaa Ala Lys atg gtg Met Val gct gaa Ala Glu tgg ttt Trp Phe ctc acg Leu Thr cgt gga Arg Gly 135 gga tgt Gly CyS 150 acc ccc Thr Pro gct gcg Ala Ala cgt gac Arg Asp tcc att Ser Ile 215 ctt ggt Leu Gly 230 gca gga Ala Gly tat tgg Tyr Trp gac gcc Asp Ala gca Ala ttg Leu atc Ile cc t Pro c tg Leu 120 ga t Asp gaa Glu t ca Ser atg Met ctg Leu 200 gtc Val1 tgg Trp gaa Glu caa Gin gtc Val1 280 acc Thr ctg Leu ccg Pro ccc Pro 105 cgc Arg gtt Val1 gta Val t tg Leu ccc Pro 185 gac Asp ccg Pro gga Gly g tg Val1 c ga Arg 265 gtt Val1 gaa Glu caa Gin t ta Leu 90 gtg Val ttg Leu tta Leu gta Vali cgg Arg 170 gtc Val1 ggg Gly tcg Ser ctt Leu atc Ile 250 tgg Trp gat Asp gcg Ala gca Ala 75 acc Thr t tc Phe gaa Glu gga Gly gaa Glu.

155 gat Asp t ta Leu cgc Arg gcg Ala ctt Leu 235 ctc Leu cgc Arg gca Ala ggt Gly 60 gaa Glu atc Ile aac Asn gat Asp gcg Ala 140 ct t Leu gcc Ala cgc Arg gtc Val gaa Glu 220 ccc Pro ctc Leu ctg Leu gca Ala 45 gaa Glu act Thr gcc Ala gag Glu gaa Glu 125 gta Val gga Gly tac Tyr t tc Phe gat Asp 205 gg t Gly gaa Glu gat Asp gaa Glu atc Ile 285 gtc Val aaa, Lys atc Ile gta Val 110 gcg Ala ac c Thr acc Thr atg Met ggt Gly 190 gg t Gly ttt Phe acc Thr gag Giu tc t Ser 270 gag Glu ctt Leu gcg Ala aac Asfl 95 gc t Ala cac His cgt Arg atg Met gtt Val 175 ccc Pro cc t Pro ggt G ly ca a Gin ata Ile 255 aga Arg gga Gly gtg Val caa Gin 80 gca Ala tc t Ser aca Thr gaa Glu cgc Arg 160 gat Asp aaa Lys gtg Val gag Giu gct Ala 240 ccc Pro tct Ser caa Gin 65 c ta Leu gat Asp tgg Trp tta Leu gct Ala 145 cac His ggg Gly gat Asp ggg Gly gca Al a 225 gc t Ala att Ile cta Leu gca Ala tct Ser tcg Ser ggt Gly tcc Ser 130 aat Asn t tg Leu aaa Lys gtg Val cgc Arg 210 att Ile ccc Pro gac Asp gct Ala gcg Ala gga Gly cta Leu gga Gly 115 ttg Leu ccc Pro gcc Ala cta Leu ctt Leu 195 agg Arg cgc Arg atg Met aca Thr aga Arg 275 cgg Arg cgc Arg t cc Ser 100 gca Ala c tg Leu gtg Val1 att Ile gat Asp 180 c aa Gin cgc Arg cga Arg cta Leu ccg Pro 260 ctc Leu aaa Lys ctt Leu aca Thr acg Thr cgg Arg gcg Ala gca Ala 165 tgg Trp gac Asp gta Val ggc Gly aaa Lys 245 atg Met aca Thr 307 355 403 451 499 547 595 643 691 739 787 835 883 931 980 ttg cgg cct Leu Arg Pro 290 tagttacttc WO 01/00843 WO 0100843PCT1IIBO0I00923 tgaaaaggtt cag 993 <210> <211> 290 <212> PRT <213> Corynebacterium glutamicun <400> Met Asn Pro Ile Gin Leu Asp Thr Leu Leu Ser Ile Ile Asp Glu Gly 1 5 10 Ser Phe Glu Gly Ala Ser Leu Ala Leu Ser Ile Ser Pro Ser Ala Val 25 Ser Gin Arg Val. Lys Ala Leu Glu His His Vai Gly Arg Val Leu Val 40 Ser Arg Thr Gin Pro Ala Lys Ala Thr Giu Ala Gly Glu Val Leu Val 55 Gin Ala Aia Arg Lys Met Val Leu Leu Gin Ala Giu Thr Lys Ala Gin 70 75 Leu Ser Gly Arg Leu Ala Giu Ile Pro Leu Thr Ile Ala Ile Asn Ala 90 Asp Ser Leu Ser Thr Trp Phe Pro Pro Val Phe Asn Giu Val Ala Ser 100 105 110 Trp Gly Gly Ala Thr Leu Thr Leu Arg Leu Glu Asp Glu Ala His Thr 115 120 125 Leu Ser Leu Leu Arg Arg Gly Asp Val Leu Gly Ala Val Thr Arg Glu 130 135 140 Ala Asn Pro Val Ala Gly Cys Giu Val Val Glu Leu Gly Thr Met Arg 145 150 155 160 His Leu Ala Ile Ala Thr Pro Ser Leu Arg Asp Ala Tyr Met Val Asp 165 170 175 Gly Lys Leu Asp Trp Ala Ala Met Pro Val Leu Arg Phe Gly Pro Lys 180 185 190 Asp Val Leu Gin Asp Arg Asp Leu Asp Gly Arg Val Asp Gly Pro Val 195 200 205 Gly Arg Arg Arg Val Ser Ile Val Pro Ser Ala Giu Gly Phe Gly Glu 210 215 220 Ala Ile Arg Arg Gly Leu Gly Trp Gly Leu Leu Pro Giu Thr Gin Ala 225 230 235 240 Ala Pro Met Leu Lys Ala Gly Giu Val Ile Leu Leu Asp Giu Ile Pro 245 250 255 Ile Asp Thr Pro Met Tyr Trp Gin Arg Trp Arg Leu Giu Ser Arg Ser 260 265 270 WO 01/00843 PCTIBOOIOO923 Leu Ala Arg Leu Thr ASP Ala Val Val ASP Ala Ala Ile Glu Gly Leu 275 280 285 Arg Pro 290 <210> 51 <211> 1626 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(1603) <223> RXA00241 <400> 51 ggtctccagc ctttctaaac aattcatctg cacttgatta attggcccca agattacgcg aagtttagcg acttcgccgt acgtcaacta cgttaaatga gtg aat act caa tca 115 Val Asn Thr Gln Ser 1 gat tct gcg ggg tct caa ggt gca gcg gcc aca agt cgt act gta tct 163 Asp Ser Ala Gly Ser Gin Gly Ala Ala Ala Thr Ser Arg Thr Val Ser 15 att aga acc ctc atc gcg ctg atc atc gga tcg acc gtc ggc gcg gga 211 Ilie Arg Thr Leu Ile Ala Leu Ile Ile Gly Ser Thr Val Gly Ala Gly 30 att ttc tcc atc cct caa aac atc ggc tca gtc gca ggt ccc ggc gcg 259 Ile Phe Ser Ile Pro Gin Asn Ile Gly Ser Val Ala Gly Pro Gly Ala 45 atg ctc atc ggc tgg ctg atc gcc ggt gtg ggc atg ttg tcc gta gcg 307 Met Leu Ile Gly Trp Leu Ile Ala Gly Val Gly Met Leu Ser Val Ala 60 ttc gtg ttc cat gtt ctt gcc cgc cgt aaa cct cac ctc gat tct ggc 355 Phe Val Phe His Val Leu Ala Arg Arg Lys Pro His Leu Asp Ser Gly 75 80 gtc tac gca tat gcg cgt gtt gga ttg ggc gat tat gta ggt ttc tcc .403 Val Tyr Ala Tyr Ala Arg Val Gly Leu Gly Asp Tyr Val Gly Phe Ser 95 100 tcc gct tgg ggt tat tgg ctg ggt tca gtc atc gcc caa gtt ggc tac 451 Ser Ala Trp Gly Tyr Trp Leu Gly Ser Val Ile Ala Gin Val Gly Tyr 105 110 115 gca acg tta ttt ttc tcc acg ttg ggc cac tac gta ccg ctg ttt tcc 499 Ala Thr Leu Phe Phe Ser Thr Leu Gly His Tyr Val Pro Leu Phe Ser 120 125 130 caa gat cat cca ttt gtg tca gcg ttg gca gtt agc gct ttg acc tgg 547 Gin Asp His Pro Phe Val Ser Ala Leu Ala Val Ser Ala Leu Thr Trp 135 140 145 ctg gtg ttt gga gtt gtt tcc cga gga att agc caa gct gct ttc ttg 595 74 WO 01/00843 Leu Val Phe 150 aca acg gtc Thr Thr Val atc ctt gtt Ile Leu Val tta tgg gcg Leu Trp Ala 200 ggc atc atg Gly Ile Met 215 tcg gta tat Ser Val Tyr 230 acc gtg att Thr Val Ile tcg ctg agc Ser Leu Ser gat aat tcc Asp Asn Ser 280 gcc gca ttg 1027 Ala Ala Leu 295 gtg tcc tgg 1075 Val Ser Trp 310 gat ggc ctc 1123 Asp Gly Leu gcc tgg atg 1171 Ala Trp Met atc att tC 1219 Gly acc Thr gca Ala 185 cgt Arg gtg Val1 tcc Ser ggt Gly ttc Phe 265 atg Met att Ile cag Gin att Ile gc t Ala 345 t tc Val1 acc Thr 170 ttc Phe ga t Asp tac Tyr cgc Arg ttt Phe 250 gg t Gly gcg Ala tcg Ser atg Met c ca Pro 330 cag Gin ctc Val 155 gtg Val1 t tg Leu ggt Gly acc Thr cag Gin 235 gtg Val gta Val tcg Ser ttg Leu c tc Leu 315 agc Ser ctg Leu aac Arg aaa Lys ttt Phe gtg Val 205 tgg Trp cgc Arg gtt Val acc Thr c tc Leu 285 ctg Leu gca Ala atc Ile tcc Ser acc Gly att Ile agc Ser 190 ggC Gly gtg Val1 t ca Ser C tc Leu caa Gin 270 gaa Giu tgt Cys gaa Giu ggg Gly acc Thr 350 acc Gin ctg Leu aag Lys ttt Phe ggt Gly 225 ga t Asp c tg Leu ctc Leu gtt Val1 gt t Val 305 gcg Ala aac Asn att Ile tc Ala ttg Leu ttc Phe gat Asp 210 atc Ile gtc Val1 g tg Val1 gc t Ala ggt Giy 290 ctt Leu ttg Leu agc Ser cag Gin atg PCTIIBOO/00923 Phe Leu 165 ttc atc 643 Phe Ile 180 gtt gat 691 Val Asp gtg cgc 739 Val Arg ggt gca 787 Gly Ala cga gct 835 Arg Ala 245 att tct 883 Ile Ser 260 tta cca 931 Leu Pro tgg ggt 979 Tip Gly gcc tat Ala Tyr gca atg Ala Met 325 ggt gCt Gly Ala 340 ttc atc Phe Ile caa ttg Ile Ile Phe Phe Leu Asn Glu Thr Thr Tyr Val Ser Met Val Gin Leu 360 365 370 WO 01/00843 WO 0100843PCT/IBOO/00923 gct acc 1267 Ala Thr 375 gtc atg 1315 Val Met 390 aca cgt 1363 Thr Arg aaa cac 1411 Lys His ttt tac 1459 Phe Tyr ctt ccc 1507 Leu Pro 455 cag gtg 1555 Gin Val 470 *gct gcc 1603 Ala Ala aac cta tac ttg gtg Asn Leu Tyr Leu Val 380 ctg gca aca cgt gga Leu Ala Thr Arg Gly 395 ttt gat gat tcc ggt Phe Asp Asp Ser Gly 410 ctc atc gtc ggt tta Leu Ile Val Gly Leu cct tac ctg ttc tct gcc ttt tat ctg Pro Tyr Leu Phe Ser Ala Phe Tyr Leu 385 aaa gga atc acc cac cca cat gcc ggc Lys Gly Ile Thr His Pro His Ala Gly cca gag ata Pro Giu Ile 415 cgc cga gaa aac cgc Arg Arg Giu Asn Arg 420 gta Val 425 gct gca Ala Ala 440 ggc tta Gly Leu ttt aac Phe Asn gaa ccg cag ttt Giu Pro Gin Phe 445 atc ccc tat gtg Ile Pro Tyr Val 460 cgc ttt gaa atc Arg Phe Giu Ile gca acg gtg tat tca gtg tgg ctg Ala Thr Val Tyr Ser Val Trp Leu 430 435 gtc ctc ttc gga gcc atg gcg atg Val Leu Phe Gly Ala Met Ala Met 450 tgg aca agg att tat cgt ggc gaa Trp Thr Arg Ile Tyr Arg Gly Giu 465 ggc gtg gtt gtt gtc ctg gtc gtt Giy Val Val Val Val Leu Val Val 475 agc gcg ggc gtt.

Ser Ala Gly Val 490 480 att ggt ttg gtc aac Ile Gly Leu Val Asn 495 485 gga tca cta tcg ctt Gly Ser Leu Ser Leu 500 taaacaccga aaccttcctg cta 1626 <210> 52 <211> 501 <212> PRT <213> Corynebacteriun giutamicun <400> 52 Val Asn Thr Gin Ser Asp Ser Ala Gly Ser Gin Gly 1 5 10 Ser Arg Thr Val Ser Ile Arg Thr Leu Ile Ala Leu 25 Thr Val Gly Ala Gly Ile Phe Ser Ile Pro Gin Asn 40 Ala Gly Pro Gly Ala Met Leu Ile Gly Trp, Leu Ile 55 Ala Ala Ala Thr Ile Ile Ala Ile Gly Ser Gly Ser Val Gly Val Gly WO 01100843 WO 0100843PCT/EBOO/00923 Met Leu Ser Vai Ala Phe Val Phe His Vai Leu Ala Arg Arg Lys Pro 70 75 His Leu Tyr Vai Ala Gin Val Pro 130 Ser Ala 145 Gin Ala Leu Leu Lys Phe Phe Asp 210 Gly Ile 225 Asp Vai Leu Val1 Leu Ala Val Gly 290 Val Leu 305 Ala Leu Asn Ser Ilie Gin Ser Met 370 Asp G iy Val1 115 Leu Leu Aia Cys Thr 195 Gin Glu Ser Ser Ala 275 Pro Gly Met Arg Ilie 355 Val Ser Phe 100 Gly Phe Thr Phe Phe 180 Val1 Vai Gly Arg Ile 260 Leu Trp Ala Ala Gly 340 Phe Gin Giy Ser Tyr Ser Trp, Leu 165 Ilie Asp Arg Ala Ala 245 Ser Pro Giy Tyr Met 325 Ala Ile Leu Val1 Ser Ala Gin Leu 150 Thr Ilie Leu Gly Ser 230 Thr Ser Asp Ala Val1 310 Asp Ala Ile Ala Tyr Ala Thr Asp 135 Val1 Thr Leu Trp Ile 215 Val Val1 Leu Asn Ala 295 Ser Gly Trp Ile Thr 375 Ala Trp Leu 120 His Phe Val1 Val1 Ala 200 Met Tyr Ile Ser Ser 280 Leu Trp Leu Met Phe 360 Asn Tyr Gly 105 Phe Pro Gly Thr Ala 185 Arg Val1 Ser Gly phe 265 Met Ile Gin Ile Ala 345 Phe Leu Ala 90 Tyr Phe Phe Val1 Thr 170 Phe Asp Tyr Arg Phe 250 Gly Ala Ser Met Pro 330 Gin Leu Tyr Arg Tip Ser Val1 Val1 155 Val1 Leu Gly Thr Gin 235 Val Val1 Ser Leu Leu 315 Ser Leu Asn Leu Val Leu Thr Ser 140 Ser Ala Gly Gly Val1 220 Ala Ala Leu Val Gly 300 Cys Lys Ile Glu Val 380 Gly Gly Leu 125 Ala Arg Lys Phe Val 205 Tip Arg Val Thr Leu 285 Leu Ala Ile Ser Thr 365 Pro Leu Ser 110 Gly Leu Gly Ile Ser 190 Gly Val Ser Leu Gin 270 Giu Cys Glu Gly Thr 350 Thr Tyr Gly Val His Ala Ile Leu 175 Trp Ser Phe Arg Leu 255 Gin Ala Leu Pro Ala 335 Ile Tyr Leu Asp Ile Tyr Val Ser 160 Pro Giu Ile Ile Ser 240 Leu Giu Val1 Ser Leu 320 Ile Val1 Val1 Phe WO 01/00843 WO 0100843PCTTBOO/00923 Ser 385 His Arg Tyr Gly Ile 465 Val1 Gly Tyr Ala Asn 420 Trp Ala Gly Val1 Ser 500 Val1 390 Thr Lys Phe Leu Gin 470 Ala Ala Asp Ile 425 Ala Leu Asn Ala Thr Asp 410 Val Giu Ile Arg Gly 490 Lys Pro Val1 Phe 445 Vai Ile Gly Gly Giu Ala 430 Vai Trp Giy Leu Ile Ile 415 Thr Leu Thr Val1 Val 495 Thr 400 Ser Val1 Phe Arg Val 480 Asn <210> 53 <211> 822 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(799) <223> RXA01394 <400> 53 gagcaaagtg tccagttgaa tggggttcat gaagctatat tcattttact taagtacttc cataggtcac gatggtgatc aca Thr aat Asn gtt Val1 ac c Thr att Ile ttg Leu att Ile tgt Cys ga t Asp ggt Gly ggg Gly aaa Lys tta Leu ctt Leu ggc Gly 75 gcc Ala c aa Gin att Ile t tg Leu atc Ile ctt Leu att Ile gac Asp aat Asn tac Tyr ctg Leu cgc Arg ttt Phe gcg Ala tta Leu 80 taaaccatgt taagaaccaa atg gaa atc ttc att Met Giu Ile Phe Ile 1 tcc atc gga ccg cag Ser Ile Gly Pro Gin gaa gga ctc att gcg Giu Gly Leu Ile Ala ttg ttc atc gcc ggc Leu Phe Ile Ala Gly ccg atc gtg ctc gat Pro Ile Val Leu Asp tgg ttt gcc gtc atg Trp Phe Ala Val Met 115 163 211 259 307 355 WO 01/00843 WO 0100843PCT1IB00I00923 aaa Lys aca Thr gcc Ala 120 cag Gin t tg Leu ggc Gly ttc Phe gca Ala 200 gtc Val1 gac Asp gaa Glu 105 act Thr cgg Arg aac Asn gcg Ala gcg Ala 185 t tg Leu gtc Val1 atg Met acc Thr acg Thr tgg Trp aat Asn 155 tac Tyr agc Ser cgc Arg gca aca Thr gtg Val1 cgc Arg gta Val1 140 gcg Ala ggc Gly ctg Leu ccg Pro gtt aac Asn ccc Pro aac Asn 125 aag Lys tat Tyr gac Asp atc Ile ctg Leu 205 gtg aag Lys gat Asp 110 cgg Arg ccc Pro t tg Leu acc Thr tgg Trp 190 t cc Ser atg gtg Val1 gac Asp gtg Val atg Met gac Asp gga Gly 175 t tc Phe agc Ser a cc gaa Giu acg Thr cgg Arg ttg Leu gcg.

Ala 160 cgg Arg ccg Pro ccc Pro gca gcg Ala cc t Pro gtg Val1 atg Met 145 ttt Phe tgg Trp ctg Leu aag Lys t tg Leu 225 cag Gin ggc Gly 115 gtg Val atc Ile ttt Phe ttc Phe ggt Gly 195 tgg Trp, atc Ile atc Ile 100 ggt Gly agc Ser gtg Val1 atc Ile gcc Ala 180 ttc Phe cgc Arg aaa Lys att Ile tcg Ser gtc Val c tg Leu ggc Gly 165 gc t Ala ggc Gly tgg Trp ctg Leu Vai Ala Val Val Met Thr Ala 220 atg ttg atg ggt tagttttcgc gggttttgga atc Met Leu Met Giy 230 <210> 54 <211> 233 <212> PRT <213> Corynebacterium glutanicun <400> 54 Met Giu Ile Phe Ile Thr Gly Leu Leu Leu Gly Ala Ser Leu Leu Leu 1 5 10 Ser Ilie Gly Pro Gin Asn Val Leu Val Ile Lys Gin Gly Ile Lys Arg 25 Giu Giy Leu Ilie Ala Val Leu Leu Vai Cys Leu Ilie Ser Asp Val Phe 40 Leu Phe Ile Ala Gly Thr Leu Gly Vai Asp Leu Leu Ser Asn Ala Ala 55 Pro Ilie Val Leu Asp Ile Met Arg Trp, Gly Giy Ile Ala Tyr Leu Leu 79 WO 01/00843 WO 0100843PCTAIBOO/00923 Trp Ala Pro Val1 Met 145 Phe Trp Leu Lys Leu 225 Ala Gin Gly 115 Val1 Ile Phe Phe Gly 195 Trp Ile Val1 Ile 100 Gly Ser Val1 Ile Ala 180 Phe Arg Lys 70 Ala Glu Ala Asp Thr 150 Gly Gly Ala Ile Met 230 Ala Glu Val Lys 135 Trp Val1 Ala Ala Asn 215 Leu Lys Thr Ala 120 Gin Leu Gly Phe Al a 200 Val1 Met Asp Glu 105 Thr Arg Asn Ala Ala 185 Leu Val1 G ly Ala Pro Asp Val1 Pro Gin 170 Ala Ser Val 75 Met Thr Thr Trp Asn 155 Tyr Ser Arg Ala Val1 Asp Val1 Met Asp Gly 175 Phe Ser Thr Giu Thr Arg Leu Ala 160 Arg Pro Pro Al a <210> <211> 1026 <212> DNA <2 13> Corynebacterium glutamicum <220> <221> CDS <222> (1003) <223> RXA00865 <400> ttatcggaat gtggcttggg cgattgttat gcaaaagttg tttaaccccc aaatgaggga agaaggtaac cttgaactct aca gct aag acc gga gta gag cac ttc ggc acc Thr Ala Lys Thr Gly Val Glu His Phe Gly Thr 15 gtt act cca ttc acg gaa tcc gga gac atc gat Val Thr Pro Phe Thr Giu Ser Gly Asp Ile Asp 30 gaa gtc gcg gct tat ttg gtt gat aag ggc ttg Glu Val Ala Ala Tyr Leu Val Asp Lys Gly Leu 45 ttaggttttt tgcggggttg atg agc aca ggt tta Met Ser Thr Gly Leu 1 gtt gga gta gca atg Val Gly Val Ala Met atc gct gct ggc cgc Ile Ala Ala Gly Arg gat tct ttg gtt ctc Asp Ser Leu Val Leu WO 01/00843 WO 0100843PCT/IBOO/00923 gcg Ala gaa Glu atc Ile gaa Glu tat Tyr att Ile Arg 150 tta Leu gcc Ala gac Asp tcc Ser agc Ser 230 cta Leu gca Ala ctt Leu ggc Gly ctg Leu gcc Ala gc t Ala tac Tyr gct Ala 135 tca Ser cct Pro acg Thr c ca Pro gta Val1 215 t tc Phe tca Ser aaa Lys cca Pro acc Thr c tc Leu gg t G ly gc t Ala tcc Ser 120 gc a Ala ggt Gly acg Thr tca Ser c ta Leu 200 att Ile gag Glu ccg Pro gc t Ala att Ile 280 act Thr aag Lys gtc Val1 gc t Ala 105 aag Lys gca Ala att Ile att Ile ttg Leu 185 aac Asn gga Gly gaa Glu ctg Leu gct Ala 265 atg Met gg t Gly gcc Ala gga Gly tct Ser ccg Pro ac a Thr cca Pro t tg Leu 170 atc Ile ctt Lieu cat His ggc Gly gta Val1 250 ctg Leu gc t Ala gaa Glu gtt Val 75 acc Thr gc t Ala agc Ser gag Glu att Ile 155 gcg Ala aaa Lys gtt Val gca Al a gac Asp 235 gct Ala cgt Arg cca Pro t cc Ser 60 cgt Arg a ac Asn ggc G ly caa Gin gtt Val 140 gag Glu gtc Val gaa Glu tgg Trp, gcc Ala 220 c tc Leu gcc Ala ctg Leu aat Asn cca Pro gag Glu aac Asn gca Ala gag Glu 125 cca Pro tc t Ser aag Lys acg Thr ctt Lieu 205 ccc Pro gtc Val c aa Gin cag Gin gag Giu 285 acg Thr gaa Glu acg Thr gac Asp 110 gga Gly att Ile gat Asp gac Asp gga Gly 190 gct Al a aca Thr cgt Arg gg t Gly ggc Gly 270 cag Gin aca Thr gtt Val cgg Arg 95 ggc Gly t tg Leu tgt Cys acc Thr gcc Ala 175 ctt Leu ttg Leu gca Al a gcg Ala cgc Arg 255 atc Ile gaa Glu acc Thr ggg Gly 80 aca Thr ctt Leu ctg Leu ctc Lieu atg Met 160 aag Lys gcc Ala ggc Gly t ta Lieu cgg Arg 240 t tg Leu aac Asn ctt Leu gcc Ala ga t Asp tct Ser tta Leu gcg Ala tat Tyr 145 aga Arg ggt Gly tgg Trp gga Gly cgt Arg 225 gaa Giu gg t Gly gta Val1 gag Glu gc t Ala cgg Arg gtg Val gtt Val cac His 130 gac Asp cgc Arg gac Asp tat Tyr tca Ser 210 gag Glu atc Ile gga Gly gga Gly gc t Ala 290 gaa Glu gcg Ala gaa Glu gta Val1 115 ttc Phe att Ile ctg Leu ctc Leu tca Ser 195 ggt Gly t tg Lieu aac Asn gtc Val gat Asp 275 ctc Leu aaa Lys aag Lys ctt Leu 100 act Thr ggt Gly cc t Pro agt Ser gtt Val1 180 ggc Gly ttc Phe tac Tyr gcc Ala agc Ser 260 cc t Pro cga Arg cta Lieu ctc Lieu gcg Ala cc t Pro gca Ala ggt Gly gaa Glu 165 gca Ala gat Asp att Ile aca Thr aaa Lys 245 t tg Leu cga Arg gaa Glu 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 WO 01/00843 WO 0100843PCTIIBOO/00923 gac atg aaa aaa gct gga gtt cta taaatatgaa tgattcccga aat 1026 Asp Met Lys Lys Ala Gly Val Leu 295 300 <210> 56 <211> 301 <212> PRT <213> Cory-nebacteriun glutamicun <400> 56 Met Ser Thr Gly Leu Thr Ala Lys Thr Gly Val Glu His Phe Gly Thr 1 5 10 Val Gly Val Ala Met Val Thr Pro Phe Thr Giu Ser Gly Asp Ile Asp 25 Ile Ala Ala Gly Arg Giu Val Ala Ala Tyr Leu Val Asp Lys Gly Leu 40 Asp Ser Leu Val Leu Ala Gly Thr Thr Gly Glu Ser Pro Thr Thr Thr 55 Ala Ala Glu Lys Leu Glu Leu Leu Lys Ala Val Arg Glu Giu Val Gly 70 75 Asp Arg Ala Lys Leu Ile Ala Gly Val Gly Thr Asn Asn Thr Arg Thr 90 Ser Val Giu Leu Ala Giu Ala Ala Ala Ser Ala Giy Ala Asp Gly Leu 100 105 110 Leu Val Val Thr Pro Tyr Tyr Ser Lys Pro Ser Gin Glu Gly Leu Leu 115 120 125 Ala His Phe Gly Ala Ile Ala Ala Ala Thr Glu Val Pro Ile Cys Leu 130 135 140 Tyr Asp Ile Pro Gly Arg Ser Gly Ile Pro Ile Giu Ser Asp Thr Met 145 150 155 160 Arg Arg Leu Ser Glu Leu Pro Thr Ile Leu Ala Val Lys Asp Ala Lys 165 170 175 Gly Asp Leu Val Ala Ala Thr Ser Leu Ile Lys Glu Thr Gly Leu Ala 180 185 190 Trp Tyr Ser Gly Asp Asp Pro Leu Asn Leu Val Trp Leu Ala Leu Gly 195 200 205 Gly Ser Gly Phe Ile Ser Val Ile Gly His Ala Ala Pro Thr Ala Leu 210 215 220 Arg Glu Leu Tyr Thr Ser Phe Giu Glu Gly Asp Leu Val Arg Ala Arg 225 230 235 240 Glu Ile Asn Ala Lys Leu Ser Pro Leu Val Ala Ala Gin Gly Arg Leu 245 250 255 Gly Gly Val Ser Leu Ala Lys Ala Ala Leu Arg Leu Gin Gly Ile Asn WO 01/00843 WO 0100843PCTJIIB00/00923 270 Giu Gin Giu Leu 285 Val Gly Asp Pro Arg Leu Pro Ilie Met 275 280 Ala Pro Asn Giu Ala Leu Arg Glu Asp Met Lys Lys Ala Giy Val Leu 290 295 300 <210> 57 <211> 1071 <212> DNA <213> Corynebacterium glutamicun <2 <221> <222> <223>

CDS

(101)..(1048) RXS02021 <400> 57 ttgggtcgcc gaggagatct aatcctggtt tgagttcaga gttcacaggt ttaagcctac aaaccttagt taaaacatga tggaagcggt cgattaaaaa atg agt gaa aac Met Ser Glu Asn cgc gga gcc caa gca gtt gga atc gca Arg Gly Ala Gin Ala Val Gly Ile Ala atc gcc atg gac Ile Ala Met Asp ggg acc Gly Thr atc ctg gac Ile Leu Asp tgg gct gaa Tip Ala Glu acg Thr tgg tao cca gaa coo caa att ttc aac cog gat cag Trp Tyr Pro Glu Pro Gin Ile Phe Asn Pro Asp Gin cgo tac cca ttg Arg Tyr Pro Leu gtg ggc acc aca cgc ctc gga gca Val Gly Thr Thr Arg Leu Gly Ala aac gaa Asn Glu cto aoc oca ogg Leu Thr Pro Arg ctg cag ttg gta Leu Gin Leu Val aaa.

Lys ctg gac caa gat Leu Asp Gin Asp ctc gtc gaa cag Leu Val Glu Gin gca gtc ogc acc Ala Val Arg Thr ato coo gat ctg Ile Pro Asp Leu caa cot cca gta Gin Pro Pro Val gac

ASP

gcg cac gat gtt Ala His ASP Val ctg cgc cto cac Leu Arg Leu His ctg ott Leu Leu 100 too cac cgg Ser His Arg gag ctg ctg Giu Leu Leu 120 o tg Leu 105 gtc cgc ccc cac Val Arg Pro His atg cac atg. caa aac acc ttg Met His Met Gin Asn Thr Leu 115 tcc gac gtg gtg Ser Asp Val Val aca aac aag Thr Asn Lys ggc cot Giy Pro 130 too cgc Ser Arg 145 tgc Ott oct Cys Leu Pro gga otc atc Gly Leu Ile gaa aac Giu Asn 135 ttt gag tgg gtg Phe Glu Trp, Val ggt got ctg cgg Gly Ala Leu Arg WO 01/00843 WO 0100843PCT/IBOO/00923 cac gtc tac tgt gtg gac cgt ctt ccc cgc atg gtc gac tat gtg gtt 595 His Val Tyr Cys Val Asp Arg Leu Pro Arg Met Val Asp Tyr Val Val 150 155 160 165 ccc cct gga gtc cgc atc tcc gaa gca gaa cgc gtg cgc cta ggt gca 643 Pro Pro Gly Vai Arg Ile Ser Giu Ala Giu Arg Val Arg Leu Gly Ala 170 175 180 tac ctt gct ccg ggt acc tct gtg ctg cgt gaa ggt ttc gtg tct ttc 691 Tyr Leu Ala Pro Gly Thr Ser Val Leu Arg Giu Gly Phe Val Ser Phe 185 190 195 aac tcc ggc acc ttg ggt gcc gca aag gtg gaa ggc cgc ctg agt tcc 739 Asn Ser Gly Thr Leu Gly Ala Ala Lys Val Glu Gly Arg Leu Ser Ser 200 205 210 ggt gtg gtc atc ggt gaa ggt tcc gag att gga ctg tct tct act att 787 Gly Val Val Ilie Gly Giu Gly Ser Giu Ilie Gly Leu Ser Ser Thr Ile 215 220 225 cag tcc ccg aga gat gaa cag cgc cgc cgt ttg ccg ttg agc atc ggc 835 Gin Ser Pro Arg Asp Glu Gin Arg Arg Arg Leu Pro Leu Ser Ile Gly 230 235 240 245 caa aac tgc aac ttt ggt gtc agc tcc gga atc atc gga gtc agt ctg 883 Gin Asn Cys Asn Phe Giy Val Ser Ser Gly Ilie Ile Gly Val Ser Leu 250 255 260 gga gac aat tgc gac atc gga aat aac att gtc ttg gat gga gat acc 931 Gly Asp Asn Cys Asp Ile Gly Asn Asn Ile Vai Leu Asp Gly Asp Thr 265 270 275 ccc att tgg ttc gca gcc gat gag gag tta cgc act atc gac tcc atc 979 Pro Ile Trp, Phe Ala Ala Asp Giu Giu Leu Arg Thr Ile Asp Ser Ile 280 285 290 gaa ggc caa gca aat tgg tca atq aag cgt gaa tcc ggc ttc cat gag 1027 Giu Giy Gin Ala Asn Trp Ser Ile Lys Arg Giu Ser Giy Phe His Giu 295 300 305 cca gtt gcc cgc ctc aaa gct tgacccattt tcataaccag tgc 1071 Pro Val Ala Arg Leu Lys Ala 310 315 <210> 58 <211> 316 <212> PRT <213> Corynebacteriun giutanicum <400> 58 Met Ser Giu Asn Ile Arg Gly Ala Gin Ala Vai Gly Ile Ala Asn Ile 1 5 10 Ala Met Asp Gly Thr le Leu Asp Thr Trp, Tyr Pro Glu Pro Gin Ile 25 Phe Asn Pro Asp Gin Trp Ala Giu Arg Tyr Pro Leu Glu Val Gly Thr 84 WO 01/00843 WO 0100843PCT/IBOO/00923 40 Thr Arg Leu Gly Ala Asn Giu Leu Thr Pro Arg Met Leu Gin Leu Val 55 Lys Leu Asp Gin Asp Arg Leu Val Giu Gin Val Ala Val Arg Thr Val 70 75 Ile Pro Asp Leu Ser Gin Pro Pro Val Asp Ala His Asp Val Tyr Leu 90 Arg Leu His Leu Leu Ser His Arg Leu Val Arg Pro His Giu Met His 100 105 110 Met Gin Asn Thr Leu Giu Leu Leu Ser Asp Val Vai Trp Thr Asn Lys 115 120 125 Gly Pro Cys Leu Pro Giu Asn Phe Giu Trp Val Arg Gly Ala Leu Arg 130 135 140 Ser Arg Gly Leu Ilie His Val Tyr Cys Val Asp Arg Leu Pro Arg Met 145 150 155 160 Val Asp Tyr Val Val Pro Pro Gly Val Arg Ile Ser Giu Ala Glu Arg 165 170 175 Val Arg Leu Gly Ala Tyr Leu Ala Pro Gly Thr Ser Val Leu Arg Giu 180 185 190 Gly Phe Val Ser Phe Asn Ser Giy Thr Leu Giy Ala Ala Lys Val Giu 195 200 205 Gly Arg Leu Ser Ser Gly Val Val Ile Gly Giu Gly Ser Glu Ile Gly 210 215 220 Leu Ser Ser Thr Ilie Gin Ser Pro Arg Asp Giu Gin Arg Arg Arg Leu 225 230 235 240 Pro Leu Ser Ilie Gly Gin Asn Cys Asn Phe Gly Val Ser Ser Gly Ile 245 250 255 Ilie Gly Val Ser Leu Gly Asp Asn Cys Asp Ilie Gly Asn Asn Ile Val 260 265 270 Leu Asp Giy Asp Thr Pro Ile Trp Phe Ala Ala Asp Giu Giu Leu Arg 275 280 285 Thr Ilie Asp Ser Ilie Giu Gly Gin Ala Asn Trp Ser Ile Lys Arg Glu 290 295 300 Ser Gly Phe His Giu Pro Val Ala Arg Leu Lys Ala 305 310 315 <210> 59 <211> 1296 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS WO 01/00843 WO 0100843PCT/EBOOIOO923 <222> (101)..(1273) <223> RXS02157 <400> 59 gggtggaatt ggcacgatgg tgctgccgga tgtttttgat cgggagaatt atcctgaagg caccgttttt agaaaagacg acaaggatgg ggaactgtaa atg agc acg ctg gaa Met Ser Thr Leu Giu act tgg cca cag Thr Trp Pro Gin att att aat acg tac ggc acc cca cca Ilie Ile Asn Thr Tyr Gly Thr Pro Pro gtt gag Val Giu ctg gtg tcc Leu Val Ser ggC Gly aag ggc gca acc Lys Gly Ala Thr act gat gac cag ggc aat gtc Thr Asp Asp Gin Gly Asn Val tac atc gac ttg ctc gcg ggc Tyr Ilie Asp Leu Leu Ala Giy gca gtc aac gcg Ala Val Asn Ala ggc cac gcc Gly His Aia cac ccg His Pro gcg atc atc gag gcg gtc acc aac cag Ala Ile Ilie Giu Ala Vai Thr Asn Gin 60 atc Ile ggc caa ctt ggt Gly Gin Leu Gly gtc tca aac ttg Val Ser Asn Leu gca tcc agg ccc Ala Ser Arg Pro gtc gag gtc gcc Val Giu Val Ala gag Giu caa Gin gag ctc atc aag Giu Leu Ile Lys ttt tcg ctt gac The Ser Leu Asp gac gcc acc ctc gcc gcg Asp Ala Thr Leu Ala Aia 100 acc cgg gtt Thr Arg Val ttc aag att Phe Lys Ilie 120 t tc Phe 105 ttc tgc aac tcg Phe Cys Asn Ser gcc gaa gca aac gag gct gct Ala Giu Aia Asn Giu Ala Ala 115 gca cgc ttg act Ala Arg Leu Thr cgt tcc cgg att Arg Ser Arg Ile o tg Leu 130 gct gca gtt Ala Ala Val cat ggt His Gly 135 ttc cac ggc cgc Phe His Gly Arg atg ggt tcc ctc Met Giy Ser Leu gcg Ala 145 ctg act ggc cag Leu Thr Giy Gin cca gac aag cgt gaa gcg ttc ctg cca atg cca agc ggt gtg gag ttc Pro Asp Lys Arg Giu Ala Phe Leu Pro Met Pro Ser Gly Val Giu Phe 150 *155 160 165 tac cct tac ggc gac acc gat tac ttg cgc Tyr Pro Tyr Giy Asp Thr Asp Tyr Leu Arg 170 175 aaa atg gta gaa acc aac Lys Met Val Glu Thr Asn 180 cca acg gat Pro Thr Asp ggc gtt gtt Gly Val Val 200 gtg Val1 185 gct gct atc ttc Ala Ala Ile Phe gag cca atc cag Giu Pro Ile Gin ggt gaa acg Gly Giu Thr 195 cgc gag ctg Arg Giu Leu cca gca cct gaa Pro Ala Pro Giu ttc ctc aag gca Phe Leu Lys Ala gtg Val1 210 WO 01/00843 WO 0100843PCTJIBOO/00923 tgc gat CYS Asp 215 gtt ggc Val Gly 230 ccc gat Pro ASP ggt gct Gly Ala aag cac Lys His aag gca 1027 Lys Ala 295 cgc aag 1075 Arg Lys 310 gta gac 1123 Val Asp gac gtc 1171 Asp Val ttg aat 1219 Leu Asn atc acc 1267 t tg Leu 220 ttc Phe gcc Ala ggc Gly ggt Gly gtc Val1 300 aag Lys ggc Gly gt t Val aac Asn gca atg Met ttt Phe aag Lys cgt Arg ggC Gly 285 gat Asp gaa Glu t tg Leu ctt Leu att Ile 365 gac atc Ile gca Ala gga Gly gca Ala 270 aac Asn gac Asp ct t Leu atg met ga t Asp 350 atc Ile gca acc Thr cat His ctt Leu 255 gc t Ala cca Pro gct Ala ctt Leu ttg Leu 335 ggt Gly cgt Arg gtc Val1 cag Gin Gly ctt Leu acc Thr 275 gca Ala gaa Glu gac Asp ctg Leu ggc Gly 355 ccg Pro gcc Ala act Thr gtt Val1 ccc Pro 260 cca Pro gc t Ala gtt Val1 Gly gag Glu 340 gt t Val ctg Leu gag Glu ggC Gly gtt Val 245 atc Ile ggC Gly gcc Ala gcc Ala gtt Val1 325 cgc Arg att Ile gtg Val aca Thr 787 835 883 931 979 Ile Thr Asp Glu Glu Ile Ala Asp Ala 375 380 atc gca taaaggactc aaacttatga ctt 1296 Ile Ala 390 <210> <211> 391 <212> PRT <213> Corynebacterium giutamicum WO 01/00843 <400> Met Ser Thr 1 Thr Pro Pro Asp Gin Gly Ala Leu Gly Ile Giy Gin Val Giu Vai Thr Leu Ala Ala Asn Glu 115 Ile Leu Ala 130 Ala Leu Thr 145 Ser Gly Val Met Val Glu Ile Gin Gly 195 Ala Val Arg 210 Glu Val Gin 225 His Asp Gly Gly Gly Leu Leu Met Thr 275 Ala Cys Ala 290 Cys Ala Glu 305 PCTIIBOO/00923 Giu 5 Giu Val Ala Gly Glu Gin Ala Val Gin Phe 165 Asn Thr Leu Gly Val1 245 Ile Gly Ala Ala Thr Leu Tyr His His 70 Glu Thr Phe His Pro 150 Tyr Pro Gly Cys Val 230 Pro Giy Lys Lys Arg Trp Val Ile Pro 55 Val Leu Arg Lys Gly 135 Asp Pro Thr Val Asp 215 Gly Asp Ala His Ala 295 Lys Pro Ser Asp 40 Ala Ser Ile Val Ile 120 Phe Lys Tyr Asp Val 200 Giu Arg Val Cys Gly 280 Val Gly Gin G ly Leu Ile Asn Lys Phe 105 Ala His Arg Gly Vai 185 Pro Tyr Thr Val Leu 265 Thr Leu Giu Vai 10 Lys Leu Ile Leu Arg 90 Phe Arg Gly Giu Asp 170 Ala Ala Gly Gly Thr 250 Ala Thr Ser Ile Gly Ala Glu Phe Phe Cys Leu Arg Ala 155 Thr Ala Pro Ile Asp 235 Met Thr Phe Val Ile Al a Gly Al a Ala Ser Asn Thr Thr 140 Phe Asp Ile Glu Leu 220 Phe Ala Gly Gly Val 300 Asn Thr Ile Val Ser Leu Ser Gly 125 Met Leu Tyr Phe Gly 205 Met Phe Lys Arg Gly 285 Asp Thr Val1 Ala Thr Arg Asp Gly 110 Arg Gly Pro Leu Leu 190 Phe Ile Ala Gly Al a 270 Asn Asp Tyr Thr Val1 Asn Pro Asp Ala Ser Ser Met Arg 175 Glu Leu Thr His Leu 255 Ala Pro Ala Leu Phe Lys Glu Leu Leu Ala WO 01/00843 WO 0100843PCTA~BOO/00923 Lys Val Asp Gly Val Val Asp Val Arg Gly Arg 325 330 Val Val Leu Glu Arg Asp Val Ala Lys Gin Ala 340 345 Lys His Gly Val Ile Leu Asn Ala Pro Ala Asp 355 360 Thr Pro Pro Leu Val Ile Thr Asp Glu Glu Ile 370 375 Ala Ile Ala Glu Thr Ile Ala 385 390 <210> 61 <211> 1008 <212> DNA <213> Corynebacteriun glutamicum <22 0> <221> CDS <222> (101)..(985) <223> RXC00733 <400> 61 acggcgaggt tgtcggtatt ggaacgcaca cgaatttgct Gly Leu Met Leu 335 Val Leu Asp Gly 350 Asn Ile Ile Arg 365 Ala Asp Ala Val 380 gaacacgtgc ggtacctacc gtgaaattgt tgaatcccaa gagactgcgc aggcgcaatc atg agt aat act gca Met Ser Asn Thr Ala ggC Gly gca Ala cat His gtt Val1 gtg Val aag Lys gc t Ala cgt Arg gga Gly acc Thr gtc Val gga Gly atc Ile gaa Glu tcc Ser cca Pro tta Leu ttg Leu ttc Phe 75 gcg Ala gac Asp gcc Al a ctt Leu ttc Phe ctg Leu atg Met gca Ala cca Pro 1 gcg Ala ttc Phe cta Leu ggt Gly ccg Pro ggt Gly ggc Gly ccg Pro gga Gly gcc Ala aaa Lys gc t Ala aaa Lys tca Ser aat Asn att Ile gtc Val1 gcc Ala ggt Gly cat His ggc Gly 115 caa Gin cta Leu c tg Leu acc Thr gcg Ala aat Asn 100 att Ile WO 01/00843 WO 0100843PCTA~BOO/00923 ttt gaa aaa Phe Giu Lys 120 tta gcc atg atc Leu Ala Met Ile gga ctg gtg atc Gly Leu Val Ile gct tat ctc Ala Tyr Leu atc ggt Ile Giy 135 agc ctg ttg tcg Ser Leu Leu Ser ttg ttc cag gcg cgg atg ctc aac cgc atc Leu Phe Gin Ala Arg Met Leu Asn Arg Ile 140 145 cgg ctg cgc atg gag gtg gag gaa aaa atc Arg Leu Arg Met Giu Val Giu Giu Lys Ile 160 165 547 595 gtg Val 150 caa agt gcc atg Gin Ser Ala Met cac cgc cta ccg His Arg Leu Pro agc tat ttc gat Ser Tyr Phe Asp tcc Ser 175 atc aaa cgt ggt Ile Lys Arg Gly gat ctg Asp Leu 180 ctt agc cgt gtg acc aac gat gtg Leu Ser Arg Val Thr Asn Asp Val 185 aat atc ggt caa Asn Ile Giy Gin tcc ctg caa Ser Leu Gin 195 atc ggt gtg Ile Giy Vai caa acc ttg Gin Thr Leu 200 tca cag gcg atc Ser Gin Ala Ile tcc cta ctg acc Ser Leu Leu Thr gtc Val 210 ttg gtg Leu Val 215 atg atg ttt atc Met Met Phe Ile tcc cca ctg ctc Ser Pro Leu Leu gca Ala 225 ctc gtg gcg ctg Leu Val Ala Leu gta Val 230 tcc att ccg gtc acc atc gtg gtc act Ser Ile Pro Vai Thr Ile Vai Val Thr 235 gtg gtt gcg agc Val Val Aia Ser tcc cag aaa ctc Ser Gin Lys Leu ttt Phe 250 gcg gaa cag tgg Ala Giu Gin Trp cag acc ggt att Gin Thr Giy Ile ttg aat Leu Asn 260 gcg cgc ctg Ala Arg Leu gga cac caa Gly His Gin 280 gag Giu 265 gaa acc tac tct Giu Thr Tyr Ser cac gcc gtg gtt His Ala Val Val aag gtt ttc Lys Vai Phe 275 aat caa gct Asn Gin Ala aag gat gtt caa Lys Asp Val Gin gaa Giu 285 gca ttc gag gaa Ala Phe Giu Giu tgt gta taaggccagc tttggtgccc agt 1008 Cys Val 295 <210> 62 <211> 295 <212> PRT <213> Corynebacterium giutamicun <400> 62 Met Ser Asn Thr Ala Giy Pro Arg Gly Arg 1 5 10 Ser His Gin Ala Asp Ala Ala Pro Asn Gin Lys Ala Gin Asn Phe Gly Pro Ser Ala Lys Arg Leu WO 01/00843 WO 0100843PCT/IBOO/00923 25 Phe Gly Ile Leu Gly His Asp Arg Asn Thr Leu Ile Phe Val Ile Phe 40 Leu Ala Val Leu Ser Val Gly Leu Thr Val Leu Gly Pro Trp Leu Leu 55 Gly Lys Ala Thr Asn Vai Val Phe Giu Gly Phe Leu Ser Lys Arg Met 70 75 Pro Ala Gly Ala Ser Lys Glu Asp Ile Ile Ala Gin Leu Gln Ala Ala 90 Giy Lys His Asn Gin Ala Ser Met Met Giu Asp Met Asn Leu Vai Pro 100 105 110 Gly Ser Gly Ile Asp Phe Giu Lys Leu Ala Met Ile Leu Gly Leu Val 115 120 125 Ile Gly Ala Tyr Leu Ile Gly Ser Leu Leu Ser Leu Phe Gin Ala Arg 130 135 140 met Leu Asn Arg le Val Gin Ser Ala Met His Arg Leu Arg Met Giu 145 150 155 160 Val Giu Giu Lys Ile His Arg Leu Pro Leu Ser Tyr Phe Asp Ser Ile 165 170 175 Lys Arg Gly Asp Leu Leu Ser Arg Val Thr Asn Asp Val Asp Asn Ile 180 185 190 Gly Gin Ser Leu Gin Gin Thr Leu Ser Gin Aia Ile Thr Ser Leu Leu 195 200 205 Thr Val Ile Gly Val Leu Val Met Met Phe Ile Ile Ser Pro Leu Leu 210 215 220 Ala Leu Val Ala Leu Val Ser Ile Pro Val Thr Ile Val Val Thr Val 225 230 235 240 Val Val Ala Ser Arg Ser Gin Lys Leu Phe Ala Giu Gin Trp, Lys Gin 245 250 255 Thr Gly Ile Leu Asn Ala Arg Leu Giu Giu Thr Tyr Ser Gly His Ala 260 265 270 Val Val Lys Val Phe Gly His Gin Lys Asp Val Gin Glu Ala Phe Glu 275 280 285 Giu Giu Asn Gin Ala Cys Val 290 295 <210> 63 <211> 426 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS WO 01/00843 <222> <223> RXC00861 <400> 63 PCTIBOOIOO923 atg Met 1 cc t Pro act Thr ggt Gly ggt Gly c ac His aag Lys aag Lys cct cac Pro His gct gcg Ala Ala cgt gat Arg Asp gaa gaa Giu Giu act gtt Thr Val tac tcc Tyr Ser gct atg Ala Met 100 ctg gct.

Leu 'Ala 115 aag Lys 5 ctg Leu gga Gly gca Ala gtt Val1 gga Gly cct Pro atc Ile atg Met cgc Arg t tg Leu t tc Phe gg t Gly ctg Leu cac His act Thr ctg Leu atg Met att Ile 40 ggt Gly cgc Arg ttg Leu ggc Gly ggt Gly 120 at t Ile gcg Ala atc Ile gtC Val1 gac Asp ttc Phe gag Giu 105 gt t Val acc Thr 10 cg t Arg ctt Leu atc Ile gcc Ala t tg Leu 90 tgg Trp aac Asn act Thr cgt Arg tct.

Ser aac Asn aag Lys 75.

tac Tyr cgc Arg cgc Arg ggt Gly gag Glu tcc Ser atg Met gtg Val1 aac Asn cac His gac Asp act Thr ca c His tcc Ser ctg Leu c ac His gcc Al a ctg Leu aac Asn 125 ggt Gly cag G ln gtt Val1 cag Gin t cg Ser cgt Arg gcc Ala gtg Val1 gag Giu a tc Ile cca Pro atc Ile ggc Gly ccg Pro aac Asn ctt Leu gca caa aac ggt gtt gtg gtt gat atg gtc aac ggt cgc gca Ala Gin Asn Gly Val Val Val Asp Met Val Asn 130 135 <210> 64 <211> 142 <212> PRT <213> Corynebacteriun glutamicum <400> 64 Met Ala Pro His Lys Val Met Leu Ile Thr Thr 1 5 10 Pro Met Ala Ala Leu Ser Arg Met Ala Arg Arg 25 Thr Val Arg Asp Gly Asp Leu Ile Ile Leu Ser 40 Gly Asn Giu Glu Ala Val Phe Gly Val Ile Asn 55 Gly Ala Thr Val Val Thr Gly Arg Asp Ala Lys 70 75 Gly Arg Ala 140 Gly Thr Gin Gly Giu Glu His Arg Gin Ile Ser Ser Leu Val Pro Met Leu Ala Gin Ile Val His Thr Ser Gly WO 01/00843 WO 0100843PCT[LBOO/00923 His Giy Tyr Ser Gly Glu Leu Leu Phe Leu Tyr Asn Ala Ala Arg Pro 90 Lys Asn Ala Met Pro Val His Giy Giu Trp Arg His Leu Arg Ala Asn 100 105 110 Lys Giu Leu Ala Ile Ser Thr Gly Val Asn Arg Asp Asn Val Val Leu 115 120 125 Ala Gin Asn Gly Val Val Val Asp Met Val Asn Gly Arg Ala 130 135 140 <210> <211> 1066 <212> DNA <213> Corynebacterium giutamicum <2 <221> CDS <222> (101)..(1066) <223> RXC00866 <400> gcatcaacgt aggagatcct cgacttccaa ttatggctcc aaatgagcag gaacttgagg ctctccgaga agacatgaaa aaagctggag ttctataaat atg aat gat tcc cga Met Asn Asp Ser Arg aat Asn cag Gin tc t Ser ga t Asp aa c Asn cg t Arg aac Asn ggc Gly cgc Arg gaa Glu aac Asn gc t Ala gc t Ala tcc Ser gcc Ala gga Gly ggC Gly aac Asn cag Gin gcg Al a caa Gin aac Asn aat Asn aac Asn cgc Arg cc t Pro gc t Ala gga Gly cgc Arg ggt G ly aac Asn ggt Giy aag Lys gtc Val1 gag Giu tcc Ser gag Giu ggt Gly aac Asn 110 ggc Gly 93 gcg Ala ttt Phe ac c Thr caa Gin tcc Ser cgt Arg agc Se r 1 cc a Pro gca Ala gga Giy tct Ser aac Asn cgt Arg aac Asn gaa Glu gat Asp gac Asp ggt Gly aac Asn gga Gly aac Asn 115 115 163 211 259 307 355 403 451 499 gga cgc cga aac gtt gtt aag Gly Arg Arg Asn Val Val Lys WO 01/00843 120 tcg atg cag Ser Met Gin 135 gca ccg gca Ala Pro Ala 150 atc ggt cgc Ilie Gly Arg gtg gac tgt Val Asp Cys ctg att ctt Leu Ile Leu 200 gat gca ttg Asp Ala Leu 215 ccc tgg ctg Pro Trp Leu 230 ttc acc ttg Phe Thr Leu ccg aag ctg Pro Lys Leu ttc aac att Phe Asn Ilie 280 ggt ctt gct 1027 Gly Leu Ala 295 atc aag ctg 1066 Ile Lys Leu 310 <210> 66 <211> 322 <212> PRT PCTJIBOO/00923 gcg Ala gg t Gly atg Met 170 gtg Val1 gac Asp gtt Val1 aag Lys ctg Leu 250 gag Giu t tc Phe aag Lys cag Gin ga t Asp c tg Leu 155 acc Thr C tC Leu ttc Phe act Thr ctg Leu 235 at t Ile gtc Vai tgg Trp act Thr act Thr 315 cag Gin tac Tyr gag Giu tct Ser 190 att Ile cac His ga t Asp aag Lys cag Gin 270 aac Asn ggt Gly ga t Asp ctg Leu ctt Leu 160 aac Asn gg t Gly ga t Asp gac Asp cca Pro 240 aag Lys aat Asn tcc Ser gtc Val cgc Arg 320 130 gag Giu Giy cgt Arg cca Pro ctg Leu 210 att Ile t tg Leu cac His gac Asp cca Pro 290 c ac His act Thr cca Pro att Ile ctg Leu ggc Giy 195 cac His gg t Gly gca Ala cgt Arg cgc Arg 275 gac Asp acc Thr cca Pro t cc Ser ctc Leu 180 gtt Val1 cgc Arg gc t Ala tcC Ser cag Gin 260 gga Giy tgc Cys gg t Gly aag Lys gaa Giu 165 atc Ile gac Asp gtc Val1 att Ile cgt Arg 245 cgt Arg ccg Pro ctt Leu gac Asp 547 595 643 691 739 787 835 883 931 979 <213> Corynebacteriun giutamicum <400> 66 Met Asn Asp Ser Arg Asn Arg Giy Arg Lys Vai Thr Arg Lys Ala Gly 1 5 10 WO 01/00843 WO 0100843PCTJIBOO/00923 Pro Al a Gly Ser Asn Arg Asn Arg Pro 145 G ly Asn Glu His His 225 Ile Giu Giu Ile Ile 305 Pro Giu Asn Ala Ala Ser Ala Giy Met 135 Pro Gly Asp Ile Ala 215 Trp Thr Lys Asn Leu 295 Lys Asn Gin Ala Gin Asn Asn Asn 120 Gin Ala Arg Cys Leu 200 Leu Leu Leu Leu Ile 280 Ala Leu His Ser Gln Gly Asn Glu 105 Arg Gly Asn Asn Gly 185 Pro Val1 Leu Ala Ile 265 Arg Ile Asp Leu Ala Gly Ser Asn G ly Giy Ala Gly Met 170 Val1 Asp Val1 Lys Leu 250 Giu Phe Lys Gin Asp Val Ala Gin 75 Arg Ala Asn Asp Leu 155 Thr Leu Phe Thr Leu 235 Ile Val1 Trp Thr Thr 315 Val Glu Ser Glu Gly Asn Giy Gln Tyr Glu Ser 190 Ile His Asp Lys Gin 270 Asn Giy Asp Phe Thir Gin Ser Arg Ser Gly Arg Aia Tyr 175 Ser Glu Glu I le Cys 255 Ser His Leu Gly Gln Ala Asp Gly Gly Gly Arg Leu Leu 160 Asn Gly Asp Asp Pro 240 Lys Asn Ser Val1 Arg 320 WO 01/00843 WO 0100843PCT/IBOO/00923 <210> 67 <211> <212> D'J <213> Co~ <220.> <221> CD~ <222> (i1 <223> RX( rynebac terium 01)..(1504) 102 095 giutamicum <400> 67 ctctcttggt cctctcccca cccattttta agtactcaag acccttccaa cagaaaggat tactccccca acaggctcaa aaatactgaa aggctcacgc aaa act gag Lys Thr Glu tcc caa aaa gca caa tta gcc cct aag Ser Gin Lys Ala Gin Leu Ala Pro Lys gca cct gaa aag Ala Pro Giu Lys cca caa Pro Gin cgc atc cgc Arg Ilie Arg tca ttc acc Ser Phe Thr ctt att tcc gtg Leu Ile Ser Val tgg cag cga cct Trp Gin Arg Pro tgg ctc acc Trp Leu Thr gaa ctt aca Giu Leu Thr gta atc agc gct Val Ile Ser Ala gct gca acg ttg Ala Ala Thr Leu ctt cct Leu Pro ctt ttg acc ggt Leu Leu Thr Gly gcc atc gat atc Ala Ile Asp Ile ctc gga aat acc Leu Gly Asn Thr gat act tta acc Asp Thr Leu Thr gac ctg ctg gac Asp Leu Leu Asp ttc act ccg agt Phe Thr Pro Ser tta agc gtg ttg Leu Ser Val Leu acc Thr agc gtc att gcc Ser Val Ilie Ala atc gtg ctt ctc Ile Val Leu Leu gcg ttg Ala Leu 100 ctt cgc tat Leu Arg Tyr agc atg ggg Ser Met Gly 120 agt caa ttt gga Ser Gin Phe Gly cga tac acc gca Arg Tyr Thr Ala ggc aag ctc Gly Lys Leu 115 cgc tca ttg Arg Ser Leu gta cag cat gat Val Gin His Asp cgg ctt aaa acg Arg Leu Lys Thr cag aac Gin Asn 135 ctc gat ggg cca Leu Asp Gly Pro cag gac tct att Gin Asp Ser Ile aca ggc caa gta Thr Gly Gin Val agt cgg tcc att tcg gat atc aac atg Ser Arg Ser Ilie Ser Asp Ile Asn Met 155 caa agc ctt gtg Gin Ser Leu Val atg ttg ccg atg Met Leu Pro Met ttg Leu 170 atc gga aat gtg gtc aag ctt gtg ctc Ile Gly Asn Vai Val Lys Leu Val Leu 175 act ttg Thr Leu 180 WO 01/00843 WO 0100843PCTIiBOO/00923 gtg atc Vai Ile ttg gtg Leu Val ttt gcg Phe Ala 215 cat gtg His Val 230 cag gaa Gin Giu ttt gcc Phe Ala atg gtt Met Vai ggt ggc 1027 Giy Giy 295 gcg ttt 1075 Ala Phe 310 tcg ggc 1123 Ser Gly atc ttt 1171 Ilie Phe ccc ctg 1219 Pro Leu gat ttc 1267 Asp Phe 375 gaa acc 1315 atg Met cct Pro 200 tcc Ser gaa Giu gac Asp cag Gin gag Giu 280 tat Tyr tc t Ser atg Met gaa Glu tca Ser 360 cgt Arg gtt c tg Leu 185 ttg Leu acg Thr gaa Glu cgc Arg cgc Arg 265 cag Gin t tg Leu tc c Ser ctc Leu gtc Val1 345 ctt Leu ggg Gly g tg gc t Ala c tg Leu tgg Trp act Thr gag Glu 250 atg Met ctt Leu gcc Al a tat Tyr atg Met 330 att Ile tcc Pro att Ile t tg atc Ile tcg Ser gcg Ala 225 gtc Val1 acc Thr gc a Ala gtc Val1 gtg Val1 305 gtg Val1 tct Ser acc Thr tcg Ser ggt G ly 385 ggc atc Ile cga Arg 210 gat Asp aag Lys gca Ala aag Lys aac Asn 290 ggc Gly gc t Ala tct Ser ga t Asp ttc Phe 370 gtt Val1 aag WO 01/00843 Glu Thr Val Val Leu Val Gly 390 395 gtg cag ctt gct gga aac ttt 1363 Val Gin Leu Ala Giy Asn Phe 410 ttt gat agc aac ggc cat cgc 1411 Phe Asp Ser Asri Gly His Arg 425 gat atc cgc agg aat ctc atc 1459 Asp Ile Arg Arg Asn Leu Ile 440 tcc tcc tcc ata ccg cga gaa 1504 Ser Ser Ser Ile Pro Arg Glu 455 460 tgatgagcag atcgaacacg cag 1527 Pro caa Gin cgc Arg 430 gt t Val1 ctc Leu Gly cca Pro 415 t tc Phe ttt Phe ga t Asp Ser 400 gac Asp gac Asp gat Asp ggg Gly Gly agc S er gac Asp gag Giu ttt Phe 465 Lys ggc Gly C tc Leu ccg Pro 450 gga Gly PCT/EB00100923 Met Ala 405 atc gcc Ile Ala 420 cac agc His Ser ttg tac Leu Tyr cag Gin 68 <211> 468 <2i2> PRT <213> Corynebacteriun giutamicum <400> 68 Met Lys Thr Giu Gin Ser Gin Lys Ala 1 Pro Arg Leu Ala Phe Val Thr Thr Giu Pro Phe Leu Thr Leu Ala Met Lys Trp Giu Gly Pro Leu Gly 115 Arg Pro Leu Leu Asn Ser Ala 100 Lys Ser 5 Gin Thr Thr Thr Gly Leu Leu Leu Arg Ser Leu Gly 70 Leu Leu Ser Gin Arg Thr 40 Leu Thr Val Tyr G ly 120 Leu Gin 25 Val1 Leu Leu Leu Ala 105 Val1 Asp Gin 10 Leu Ile Thr Thr Thr 90 Ser Gin Gly Leu Ile Ser Gly Thr 75 Ser Gin His Pro Sar Ala Ser Aia Gly Asp Val1 Phe Asp Gly 140 Asp Pro Val1 Leu Ala Leu Ile Gly Val1 125 Gin Ile Lys Ala Ala Ile Leu Ala Arg 110 Arg Asp Asn Lys Trp Ala Asp Asp Leu Arg Leu Ser Met Ala Gin Thr Ile Arg Ile Tyr Lys Ile Val 130 Arg Thr Gly Gin Val Val Ser Arg Ser Ile WO 01/00843 WO 0100843PCT/IBOO/00923 Ser Val1 Ile Arg 210 Asp Lys Ala Lys Asn 290 Giy Ala S er Asp Phe 370 Val Lys Gly Leu Pro 450 G ly Leu Leu Ala 195

LYS

Leu Ala Arg Phe 275 Ile Thr Arg Val1 Pro 355 Asn Gin Thr His Thr 435 Phe Cys Val1 Thr 180 Aia Aia Thr Phe Giu 260 Ile Val1 Phe Ser Giu 340 Ala Asn Ala Met Ile 420 His Leu Gin Leu Ile Vai Ala 215 Val Giu Ala Vai Gly 295 Phe Gly Phe Leu Phe 375 Thr Gin Asp Ile Ser 455 Pro Met Pro 200 Ser Giu Asp Gin Giu 280 Tyr Ser Met Giu Ser 360 Arg Val1 Leu Ser Arg 440 Ser Met Leu 185 Leu Thr Giu Arg Arg 265 Gin Leu Ser Leu Vai 345 Leu Gly Val1 Ala Asn 425 Arg Ile Leu 170 Ala Leu Trp Thr Giu 250 Met Leu Ala Tyr Met 330 Ile Pro Ile Leu Gly 410 Gly Asn Pro 155 Ile Ile Leu Ser Val1 235 Thr Arg Pro Met Leu 315 Arg Asp Asp Leu Val1 395 Asn His Leu Arg Gly Ser Trp, Ala 220 Thr Asp Thr Gin Thr 300 Thr Val Leu Thr Asn 380 Gly Phe Arg Ile Giu 460 160 Lys Thr Tyr Al a Val1 240 Leu Thr Val Thr Ala 320 Leu Arg Leu Leu Ser 400 Asp Asp Asp Gly WO 01/00843 WO 0100843PCTIBOOOO923 <210> 69 <211> 295 <212> DNA <213> Corynebacterium giutamicut <220> <221> CDS <222> (84)..(272) <223> RXC03185 <400> 69 agcgcccaac cgttcagacc agcggtttct ctgaggatgc aaagtccatg atgggtnagg tcactgagct gtccgaaacc accatg aat gat ctt gca gct gaa ggt gaa aac Met Asn Asp Leu Ala Ala Giu Gly Giu Asn 1 5 gat cct tac cgc atg gtt cag cag ctg cgc cgc aag ctc tct cgc ttc Asp Pro Tyr Arg Met Val Gin Gin Leu Arg Arg Lys Leu Ser Arg Phe 20 gtc gag cag aag tgg aag cgc cag ccg gtc atc atg cca acc gtc att Vai Glu Gin Lys Trp Lys Arg Gin Pro Vai Ile Met Pro Thr Val Ile 35 ccg atg act gcg gaa acc acg cac atc ggt gac gat gag gtt cgc gct Pro Met Thr Ala Giu Thr Thr His Ile Gly Asp Asp Giu Val Arg Ala 50 tca cgc gag tcc ctg taaaagcatt'tcgcttttcg acg Ser Arg Giu Ser Leu <210> <211> 63 <212> PRT <213> Corynebacteriun giutanicun <400> Met Asn Asp Leu Ala Ala Giu Giy Giu Asn Asp Pro Tyr Arg Met Vai 1 5 10 Gin Gin Leu Arg Arg Lys Leu Ser Arg Phe Val Giu Gin Lys Trp Lys 25 Arg Gin Pro Val Ile Met Pro Thr Val Ile Pro Met Thr Ala Giu Thr 40 Thr His Ile Giy Asp Asp Giu Vai Arg Ala Ser Arg Glu Ser Leu 55 <210> 71 <211> 4653 <212> DNA <213> Corynebacteriun giutamicum <22 0> WO 01/00843 WO 0100843PCT/IBOO/00923 <221> CDS <222> (101)..(4630) <223> RXN00367 atcgaaagta acccttttgt tacttgcgtt gcaggtagtg gaacgattga tagaaacagg attaaagtga ggtatcccgc tccctgattt tcttattatc atg aaa cca caa Met Lys Pro Gin 1 ctc tac aac cct Leu Tyr Asn Pro cat gaa cat gac His Giu His Asp tgc ggt gtg gcg Cys Gly Val Ala ttt att Phe Ile gcg gat atc Ala Asp Ile gag gcg ctt Giu Ala Leu c ac His ggt cga ccc agc Gly Arg Pro Ser agc att gtt gat Ser Ile Val Asp cgt gca ctt Arg Ala Leu gca gag aag Ala Giu Lys cgc aac att gac Arg Asn Ile Asp cga ggt gcc gcc Arg Gly Ala Ala aac act Asn Thr ggc gat ggt gcg Giy Asp Gly Ala atc ctc atg cag att ccg gac ggc ttt Ile Leu Met Gin Ilie Pro Asp Gly Phe tat Tyr cgt gaa gta tct Arg Giu Val Ser att gag ctt cct Ile Giu Leu Pro gag Giu gca ggg gag tat Ala Gly Giu Tyr act ggt att gcg Thr Gly Ilie Ala ttg cct cgc ggt Leu Pro Arg Gly atg gcg atg atg Met Ala Met Met gat gct Asp Ala 100 cag aag gaa Gin Lys Giu ggt tgg cgc Giy Trp Arg 120 att Ile 105 gag cgc atc gca Giu Arg Ile Ala caa gaa ggt gcc Gin Giu Gly Ala gat gtg ctt Asp Val Leu 115 tcc atg gct Ser Met Ala atg gtt cct ttt Met Val Pro Phe tct cgt gat ttg Ser Arg Asp Leu gag gag Giu Giu 135 gcg atg cct agt Ala Met Pro Ser gcg cag att ttc Ala Gin Ile Phe ctt Leu 145 act gtg cct gga Thr Val Pro Gly aaa Lys 150 tct ggt gaa gat Ser Gly Giu Asp gac cgt gtg atg Asp Arg Val Met ttt atc cgt aag Phe Ile Arg Lys tgt gag cgt gag Cys GiU Arg Giu ggc acc acc aat Gly Thr Thr Asn gg t Gly 175 cgc gat acg gtg Arg Asp Thr Val tat ttc Tyr Phe 180 ccg tcg cta tct tca cgc acc atc Pro Ser Leu Ser Ser Arg Thr Ile 185 tac aaa ggc atg Tyr Lys Gly Met ttg acc act Leu Thr Thr 195 ctg cag ctt gag ggc ttc ttt gag gat ctg ggt gat gct cgc ctg gag Leu Gin Leu Glu Gly Phe Phe Giu Asp Leu Gly Asp Ala Arg Leu Giu WO 01/00843 WO 010084PCT/IBOO/00923 tcg gcc Ser Ala 215 agc tgg Ser Trp, 230 atc aac Ile Asn ctt atc Leu Ile tgc acc Cys Thr ctt ttg 1027 Leu Leu 295 atc cct 1075 Ile Pro 310% gat ttc 1123 Asp Phe gca gcg 1171 Ala Ala cgt aat 1219 Arg Asn gtt gtg 1267 Val Val 375 gtc gta 1315 Val Val 390 act gcc 1363 Thr Ala 200 att Ile ccg Pro act Thr aaa Lys ccg Pro 280 cac Hius cag Gin tac Tyr ctg Leu ggc G ly 360 atg Met aag Lys gag Glu cgt Arg cgt Arg aac Asn aat Asn 270 gcg Ala ctt Leu aag Lys ctg Leu cgt Arg 350 atc Ile gtg Val cct Pro gac Asp ttC Phe t tc Phe tgg Trp, 255 ttg Leu cgt Arg ccg Pro acg Thr atg Met 335 ttt Phe acc Thr t tg Leu gga Gly gag Glu tcc Ser gtt Val1 240 atg Met agc Ser ttc Phe cat His ctg Leu 320 gag Glu gtg Val1 at t Ile gac Asp cgc Arg 400 gaa Glu acg Thr 225 gcc Ala cgc Arg agc Ser gac Asp gc t Ala 305 agc Ser cca Pro ggt Gly act Thr ttg Leu 385 atg Met atc Ile 210 aac Asn cac His gcC Ala gtg Val1 gag Giu 290 gtt Val1 cct Pro t gg Trp gcc Ala gat Asp 370 agg Arg ttC Phe aag Lys t tc Phe ggt Gly gag Glu 260 cct Pro t tg Leu atg Met ctg Leu ggt Gly 340 ctg Leu ggt Gly gag Giu gtt Val aaa Lys cca Pro gag Glu 245 gcg Ala a tc Ile gag Glu atg Met cg t Arg 325 cc t Pro gac Asp t tg Leu agc Ser gac Asp 405 t ta Leu 410 WO 01/00843 WO 0100843PCTIIBOO/00923 agc gaa 1411 Ser Giu ctg gat 1459 Leu Asp gct gtg 1507 Ala Val 455 ttg ttg 1555 Leu Leu 470 atg ggt 1603 Met Gly ctt tat 1651 Leu Tyr ttg gac 1699 Leu Asp ggt gcg 1747 Gly Ala 535 atc cgt 1795 Ile Arg 550 atc aat 1843 Ile Asn att tct 1891 Ile Ser gcg att 1939 Ala Ile gcg cag Ala Gin 425 cca tat ggt gag tgg Pro Tyr Gly Glu Trp 430 cgt Arg 440 ttg Leu ctg Leu ctg cct cag aca cgc Leu Pro Gin Thr Arg 445 cgt cag cgt gtt ttc Arg Gin Arg Val Phe 460 ctg ccg atg gcc cgc Leu Pro Met Ala Arci t ac Tyr gga Gly att cgc gat aat ttt gtg cat Ile Arg Asp Asn Phe Val His 435 aac tac atg gcg cac tct cgt Asn Tyr Met Aia His Ser Arg 450 atc act gaa gaa gat gtg gat Ile Thr Glu Glu Asp Val Asp cag ggt Gin Gly tcg gat acg Ser Asp Thr 490 gat ttc ttc Asp Phe Phe 475 465 gct gag gcg att ggt tcc Ala Giu Ala Ile Gly Ser 480 485 tcc cag cga cca cgc atg Ser Gin Arg Pro Arg Met cca att gcg gcg cta Pro Ile Ala Ala Leu 495 gcg cag cgc ttt gct Ala Gin Arg Phe Ala 510 gaa aag cct gtg acc Glu Lys Pro Val Thr 500 tct Ser 520 cag Gin 505 atc cgc Ile Arg cag gtg aca aac cca ccg Gin Val Thr Asn Pro Pro 515 agc atg ttc act ttg ttg Ser Met Phe Thr Leu Leu 530 cct gat gcg gcg cga cgt Pro Asp Ala Ala Arg Arg 525 tct gac gtg ctc aat Ser Asp Val Leu Asn ccg ggt Pro Giy 545 ttg gaa tcg ccg Leu Giu Ser Pro 555 gcc aac gcg cat Ala Asn Aia His 570 atc att gat aac cat Ile Ile Asp Asn His 560 ggt gag tgg gat tcc Gly Giu Trp Asp Ser gag ctg gcc acc ttg Giu Leu Ala Thr Leu 565 ttt ggt gct gct gta Phe Gly Ala Ala Val 580 gcc ggc atg aag gct Ala Gly Met Lys Ala 595 ggt ttg Gly Leu 585 gct cgt Ala Arg 600 tac cca gtg gct cac Tyr Pro Val Ala His 590 gtg cgc cgc gag gtt Val Arg Arg Glu Val 605 tct gaa gca atc cgc aat ggc Ser Glu Ala Ile Arg Asn Giy 610 WO 01/00843 aag acg ttg 1987 Lys Thr Leu 615 cct atc cct 2035 Pro Ile Pro 630 cag caa cgt 2083 Gin Gin Arg gcc cgc gag 2131 Ala Arg Giu gcg atc aac 2179 Ala Ile Asn 680 aag ggt cag 2227 Lys Gly Gin 695 atc aag gca 2275 Ile Lys Ala 710 att gca acg 2323 Ile Ala Thr ggt ctg cac 2371 Gly Leu His cca att tct 2419 Pro Ile Ser 760 cgt cac cgc 2467 Arg His Arg 775 gaa ttg gat 2515 Giu Leu Asp 790 atc Ile gcg Ala acc Thr gtt Val1 665 ccg Pro ttg Leu gcc Ala gtg Vali cag Gin 745 ggC Gly agc Ser ttg Leu ctg Leu c tg Leu 635 acc Thr c ac His atg Met ga t Asp act Thr 715 tcg Ser C tC Leu gg t Gly ttt Phe ggt Gly 795 tcg Ser 620 ctg Leu cag Gin c tg Leu gca Ala ctt Leu 700 ggt G ly tac Tyr ctg Leu ctg Leu t tg Leu 780 gaa Glu cgt Arg tcc Ser tC Ser atg Met 670 gaa Giu ttg Leu ctg Leu ggc Gly aac Asn 750 gaa Glu cgC Arg aag Lys gaa Giu gc t Ala ctg Leu 655 ctc Leu acc Thr gat Asp aag Lys gcg Ala 735 tac Tyr gt t Val1 cc t Pro tgg Trp, tc t Ser gtg Val1 640 gtg Val1 att Ile atc Ile gag Giu gtg Val 720 cag Gin t tc Phe gca Ala gaa Giu cgc Arg 800 gat Asp 625 cat His gtg Val1 ggt Giy gat Asp gca Aia 705 atg Met ctt Leu gg t Giy gc t Ala gag Glu 785 cgc Arg cgc Arg tac Tyr tcc Ser ggt Gly 675 ctg Leu cga Arg aag Lys gat Asp att Ile 755 gta Val1 gct Ala gg t Gly PCT/iBOO/00923 atg gca Met Ala ttg gtg Leu Val 645 ggc gat Gly Asp 660 gcc gat Aia ASP cgc atg Arg Met aac tac Asn Tyr atg ggc Met Giy 725 gtc act Val Thr 740 gct tca Ala Ser gaa gct Giu Ala cac cgt His Arg gaa tac Giu Tyr 805 WO 01/00843' WO 1/0843PCTJIBOO/00923 cac ctg 2563 His Leu tct ggc 2611 Ser Gly caa tcc 2659 Gin Ser gac cgc 2707 Asp Arg 855 gtg aag 2755 Val Lys 870 gaa gcc 2803 Giu Ala tcc aac 2851 Ser Asn ccc aac 2899 Pro Asn cgt ttc 2947 Arg Phe 935 atc aag 2995 Ile Lys 950 cca aac 3043 Pro Asn ggc gtt 3091 Gly Val ttc aac cca Phe Asn Pro 810 agc tac gag Ser Tyr Glu gaa acc atc ttc aag Giu Thr Ile Phe Lys 815 att ttc aag gat tac Ile Phe Lys Asp Tyr 830 ggt act att cgt gga Gly Thr Ile Arg Gly ctg cag cat gca acg cgt Leu Gin His Ala Thr Arg 820 acc cgc aag gtt gat gat Thr Arg Lys Val Asp Asp 835 ctg ttt gag ttc agc acg Leu Phe Glu Phe Ser Thr 825 act cgc Thr Arg 840 ttg Leu 845 aag cca att tcg gtg Lys Pro Ile Ser Val 860 cgt ttc tcc act ggt Arg Phe Ser Thr Giy 875 cat gag gtc ttg gcc His Giu Val Leu Ala 890 tcc ggc gaa ggt ggc Ser Gly Giu Gly Giy tc t Ser gag gtg gag ccg Giu Val Giu Pro 865 gcg atg tct tat Ala Met Ser Tyr 880 atc gcc atg aac Ile Ala Met Asn 895 gag gac gcc cgc Giu Asp Ala Arg ggc Gly cga Arg 850 gtc agt gag atc Val Ser Giu Ile tcg att tct gct Ser Ile Ser Ala 885 ctg ggc ggt atg Leu Gly dly Met 900 cga ttt gat gtg gaa Arg Phe Asp Val Giu 915 ggt Gly 920 ggc Gly 905 910 gac tgg aag cgc Asp Trp Lys Arg gtg acc agc cac Val Thr Ser His tct Ser 925 gcc att aag cag gtg gcc tcg gga Ala Ilie Lys Gin Val Ala Ser Gly 930 atg gca cag ggc gca Met Ala Gin Gly Ala 955 aag gtg tac cca tgg Lys Val Tyr Pro Trp, tac ttg aac aac tgc Tyr Leu Asn Asn Cys 945 aag ccc ggt gaa ggt Lys Pro Gly Giu Gly 960 gtt gca gaa gtc cgc Val Ala Giu Val Arg 975 acc gat att cag Thr Asp Ile Gin ggc cag ctg cca Gly Gin Leu Pro 965 atc acc acc cca Ile Thr Thr Pro 980 att tac tcc att Ile Tyr Ser Ile 995 ggt ctg Gly Leu 985 tcc cct cca cca Ser Pro Pro Pro 990 cac cac gat His His Asp WO 01/00843 WO 0100843PCT/EBOO/00923 gag gat 3139 Giu Asp gca cga 3187 Ala Arg 1015 gcc gca 3235 Ala Ala 1030 cac gat 3283 His Asp ggt ggt 3331 Giy Gly ctc aac 3379 Leu Asn aaa act 3427 Lys Thr 1095 ctg Leu 1000 atc Ile ggt Gly ggC Gly cca Pro ggC Gly 1080 Gly gct cag ctg atc cac gac ctg aag aac gct aac cca cgc Ala Gin Leu Ile His Asp Leu Lys Asn Ala Asn Pro Arg 1005 1010 cac gtg aag cta gtg gca gaa caa ggc gtg ggc acc gtt His Val Lys Leu Val Ala Glu Gin Gly Val Gly Thr Val 1020 1025 gtg tcc aaa gca cac gct gat gtg gtg ctt att tcc ggc Val Ser Lys Ala His Ala Asp Val Val Leu Ile Ser Gly 1035 1040 gga act ggc gca tct cct ttg acc tcc ctg aag cat gcc Giy Thr Gly Ala Ser Pro Leu Thr Ser Leu Lys His Ala 1050 1055 1060 tgg gag ttg ggc ttg gct gaa acc cag caa acg ttg ctg Trp Glu Leu Gly Leu Ala Giu Thr Gin Gin Thr Leu Leu 1065 1070 -1075 ctg cgc gat cgt att cgc gtg cag tgc gat ggt cag ctg Leu Arg Asp Arg Ile Arg Val Gin Cys Asp Gly Gin Leu 1085 1090 cga gac gtg gtt atc gca gct ctt ctc ggt gcc gaa gaa Arg Asp Val Val Ile Ala Ala Leu Leu Gly Ala Glu Giu 1100 1105 gcc acc gca ccg ctg gtg gtt gaa ggc tgc atc atg atg Ala Thr Ala Pro Leu Val Val Glu Giv Cs Ile Met Met 1.045 1125 ttc ggt ttt 3475 1110 cgc gtc tgc cac ctg 3523 Arg Val Cys His Leu 1130 ccg gat ttg cgt tcc 3571 Pro Asp Leu Arg Ser 1145 ttc ttc acc ttc atc 3619 Phe Phe Thr Phe Ile 1115 1120 gac acc tgc ccg gtg ggt atc gct acc cag aac Asp Thr Cys Pro Val Gly Ile Ala Thr Gin Asn 1135 1140 aag ttc acc ggc aag gct gaa cac gtg gtc aac Lys Phe Thr Gly Lys Ala Glu His Val Val Asn 1150 1155 gcc cag gaa gtc cgt gag tac ttg gca cag ctt Ala Gin Giu Val Arg Giu Tyr Leu Ala Gin Leu 1165 1170 gat gaa gcc gtc gga caa gcc cag gtg ctg cgc 1160 cgc tct att ggt ttc 3667 Gly Phe Arg Ser Ile Asp Glu Ala Val Gly Gin Ala Gin Val Leu Arg 1175 1180 1185 WO 01/00843 WO 0100843PCT/IBOO/00923 aag cgt 3715 Lys Arg 1190 tcc Ser agc cca att 3763 Ser Pro Ile gtg cgt tgc 3811 Val Arg Cys aac gca ttt 3859 Asn Ala Phe 1240C ggt gtg gaa 3907 Gly Val Giu 1255 cgt tca gtt 3955 Arg Ser Val 1270 gcc caa ggt 4003 Ala Gin Gly gga atc cca gct gat tcc cgc gca gca Gly Ilie Pro Ala Asp Ser Arg Ala Ala 1195 1200 ttc cat cgc cca gaa act cca cac ttc Phe His Arg Pro Giu Thr Pro His Phe 1210 1215 acc aag acc cag gaa cac agc cta gaa Thr Lys Thr Gin Giu His Ser Leu Giu 1225 1230 att gat aag gct tcg gac acg atc acc Ile Asp Lys Ala Ser Asp Thr Ile Thr 1245 acc agc att gtt att gat agc tcc atc Thr Ser Ile Val Ile Asp Ser Ser Ile 1260 l ggc acg atg ctg ggt tct gca gtc agc Gly Thr Met Leu Gly Ser Ala Val Ser 1275 1280 ttg cca gac ggc acc atc acc ttg aat Leu Pro Asp Gly Thr Ile Thr Leu Asn cac ctg gat ttg His Leu Asp Leu 1205 cca act cag gat Pro Thr Gin Asp 1220 aaa gcc ctg gac Lys Aia Leu Asp 1235 cgt gcc gca gcg Arg Ala Ala Ala 1250 agc aac gtc aac Ser Asn Val Asn cgc gtg gct ggt Arg Vai Ala Giy ctt caa ggc tgc Leu Gin Gly Cys 1300 1285 gcc ggt 4051 Ala Gly 1290 aac tcc ttt Asn Ser Phe 1305 1295 ggc gcg ttc atc cca cga Gly Ala Phe Ile Pro Arg 1310 ggc atc acc atc aac Gly Ile Thr Ile Asn 1315 ctc acc ggc gat gcc aat gac: ttt 4099 Leu Thr Gly Asp Ala Asn Asp Phe 1320 132 aag att gtg atc aag cct tcc gct 4147 Lys Ile Val Ile Lys Pro Ser Ala 1335 1340 aat cca aat atc att gcc gga aac 4195 Asn Pro Asn Ile Ile Ala Gly Asn 1350 1355 ggt gaa.ttg ttc att cgt ggc cag 4243 Gly Giu Leu Phe Ile Arg Gly Gin 1370 gtg ggc aag gga tta tct ggc gga Val Gly Lys Gly Leu Ser Gly Gly 5 1330 cag gct ccg aag cag ctg aag aac Gin Ala Pro Lys Gin Leu Lys Asn 1345 gtg ctt gga tac ggc gca acc agt Val Leu Gly Tyr Gly Ala Thr Ser 1360 gtc ggc gaa cgt ttc tgc gtc cgt Val Gly Glu Arg Phe Cys Val Arg 1375 1380 1365 WO 01/00843 WO 0100843PCT/iBOO/00923 aac tct ggc gcc acc 4291 Asn Ser Gly Ala Thr 1385 gca gtg gtt gaa Ala Val Val Giu 13~ gag tac 4339 Glu Tyr aac ttt 4387 Asn Phe 1415 ccg gac 4435 Pro Asp 1430 ctg agc 4483 Leu Ser cgc gaa 4531 Arg Giu aaa atc 4579 Lys Ile gcc cac 4627 Ala Hius 1495 atg act ggc ggc cga gtc ctg Met Thr Gly Gly Arg Val Leu 1400 1405 ggt gcc ggc atg tct ggt ggc Gly Ala Gly Met Ser Gly Gly 1420 cta aac cag aag atc aat ggc Leu Asn Gin Lys Ile Asn Gly ggt atc gga aac cac ggt tgt Gly Ile Gly Asn His Gly Cys 90 1395 gtt ttg ggc ccg gtt ggt gag Val Leu Gly Pro Val Gly Glu 1410 att gca tac ctg gct aat tcc Ile Ala Tyr Leu Ala Asn Ser 1425 gaa ttg gtg gat gtt gtt cca Giu Leu Val Asp Val Val Pro 1440 143 gct gac gat ctg Ala Asp Asp Leu 1450 ctc acc gga tcc Leu Thr Gly Ser 5 1445 1465 atg ccg Met Pro acg tgg gct gat g Thr Trp Ala Asp G 1455 gag acc aag ctg c Giu Thr Lys Leu A 1470 ttc caa aaa gta c' Phe Gin Lys Val Li 1485 gac cca gca atc a ag ctc att gct cgc cac lu Leu Ile Ala Arg His 1460 gt gca caa gat ttg gtg rg Ala Gin Asp Leu Val 1475 tc aac atc atc gaa acg eu Asn Ile Ile Glu Thr 1490.

ag atc atg gag gca gtg cgc gat Arg Asp 1480C gc t Ala gag ggc caa Giu Gly Gin Asp Pro Ala Ile Lys Ile Met Glu Ala Val 1500 1505 agc taatggccga cccacaagga ttc 4653 Ser 1510 <210> 72 <211> 1510 <212> PRT <213> Corynebacterium giutamicum <400> 72 Met Lys Pro Gin Gly Leu Tyr Asn Pro 1 5 Gly Val Ala Phe Ile Ala Asp Ile His 25 Val Asp Arg Ala Leu Giu Ala Leu Arg 40 Ala His Giu His Asp Ala Cys 10 Gly Arg Pro Ser Arg Ser Ile Asn le Asp His Arg Gly Ala WO 01/00843 WO 0100843PCT/EBOO/00923 Ala Giy Ala Giu Lys Asn Thr Gly Asp Giy Ala Giy Ile Leu Met Gin 55 Ilie Pro Asp Gly Phe Tyr Arg Glu Val Ser Gly Ile Giu Leu Pro Glu 70 75 Ala Gly Glu Tyr Ala Thr Gly Ile Ala Phe Leu Pro Arg Gly Arg Met 90 Ala Met Met Asp Ala Gin Lys Giu Ile Giu Arg Ile Ala Lys Gin Giu 100 105 110 Gly Ala Asp Val Leu Gly Trp Arg Met Val Pro Phe Asp Ser Arg Asp 115 120 125 Leu Giy Ser Met Ala Giu Giu Ala Met Pro Ser Phe Aia Gin Ile Phe 130 135 140 Leu Thr Val Pro Gly Lys Ser Giy Giu Asp Leu Asp Arg Vai Met Phe 145 150 155 160 Phe Ile Arg Lys Arg Cys Giu Arg Glu Leu Giy Thr Thr Asn Gly Arg 165 170 175 Asp Thr Vai Tyr Phe Pro Ser Leu Ser Ser Arg Thr Ile Ile Tyr Lys 180 185 190 Gly met Leu Thr Thr Leu Gin Leu Giu Giy Phe Phe Giu Asp Leu Gly 195 200 205 Asp Ala Arg Leu Giu Ser Ala Ile Ala Ile Vai His Ser Arg Phe Ser 210 215 220 Thr Asn Thr Phe Pro Ser Trp Pro Leu Ala His Pro Tyr Arg Phe Val 225 230 235 240 Ala His Asn Gly Giu Ile Asn Thr Val Arg Gly Asn Giu Asn Trp Met 245 250 255 Arg Aia Arg Giu Ala Leu Ile Lys Asn Asp Lys Leu Giy Asn Leu Ser 260 265 270 Ser Val Leu Pro Ile Cys Thr Pro Giu Gly Ser Asp Thr Ala Arg Phe 275 280 285 Asp Giu Ala Leu Giu Leu Leu His Leu Gly Gly Tyr Ser Leu Pro His 290 295 300 Ala Val Ala Met Met Ilie Pro Gin Ala Trp Glu His Asn Lys Thr Leu 305 310 315 320 Ser Pro Giu Leu Arg Asp Phe Tyr Glu Tyr His Ser Cys Leu Met Giu 325 330 335 Pro Trp, Asp Gly Pro Ala Ala Leu Ala Phe Thr Asp Gly Arg Phe Val 340 345 350.

Giy Ala Val Leu Asp Arg Asri Gly Leu Arg Pro Gly Arg Ile Thr Ile 355 360 365 Thr Asp Ser Gly Leu Val Val Met Ala Ser Giu Ser Giy Val Leu Asp WO 01/00843 WO 0100843PCTIBOOIOO923 Leu 385 Met Ile Asp Met Giu 465 Giu Gin Vai Met Asp 545 Giu Phe Ala Ala Asp 625 His Val1 Gly Asp Giu Val1 Lys 420 Val1 Ser Vali Giy Arg 500 Pro Leu Arg Thr Ala 580 Lys Asn Met Leu Gly 660 Ala Arg Ser Asp 405 Leu His Arg Asp Ser 485 Met Pro Leu Arg Leu 565 Val1 Ala Gly Ala Val 645 Asp Asp Met 375 Val1 Ala Giu Asp Val 455 Leu Gly Tyr Asp Ala 535 Arg Asn Ser Ile Thr 615 Ile Gin Arg Ile Gly 695 Arg Giy Gin 425 Leu Arg Leu Asp Phe 505 Ile Ser Glu Asn Leu 585 Arg Ile Ala Thr Vai 665 Pro Leu Arg 395 Ile Tyr Gin Arg Met 475 Pro Aia Giu Val1 Pro 555 His Pro Arg Leu Leu 635 Thr His Met Asp 380 Val1 Val1 Gly Thr Val1 460 Ala Ile Gin Lys Leu 540 Ile Gly Val1 Arg Ser 620 Leu Gin Leu Ala Leu 700 Gin Giu Glu Arg 445 Phe Arg Ala Arg Pro 525 Asn Ile Giu Ala Glu 605 Asp Thr Cys Aia Phe 685 Ser Pro Asp Trp 430 Tyr Gly Gin Aia Phe 510 Val1 Pro Asp Trp His 590 Val1 Arg Ser Ser Met 670 Glu Leu G ly Giu 415 Ile Asn Ile Giy Leu 495 Ala Thr Gly Asn Asp 575 His Ser Giu Ala Leu 655 Leu Thr Asp Arg 400 Giu Arg Tyr Thr Ala 480 Ser Gin Ser Pro His 560 Ser Giy Giu Ser Vali 640 Vai Ile Ile Glu WO 01/00843 WO 0100843PCTIBOO/00923 Ala 705 Met Leu Gly Ala Giu 785 Arg Gin Arg Phe Pro 865 Gly Arg Arg Gin Cys 945 Gly Arg Asp Asn Tyr Ile Lys Ala Ala Thr Thr Gly Val Leu Lys Val 710 715 Ala Leu Ile His 775 Leu Leu Giy Ser Arg 855 Lys Ala Asn Asn Phe 935 Lys Asn Val1 Asp Thr Val His Gin 745 Ser Gly 760 Arg Ser Asp Leu Phe Asn Ser Tyr 825 Thr Arg 840 Lys Pro Arg Phe His Giu Ser Gly 905 Gly Asp 920 Gly Val Met Ala Lys Val Gly Leu 985 Leu Ala 1000 Arg Gly Asp Asn 750 Asp Giu 765 Pro Arg Tyr Lys Ile Phe Lys Asp 830 Ile Arg 845 Ser Giu Ala Met Ile Ala Giu Asp 910 Ser Ala 925 Tyr Leu Lys Pro Val Ala Pro Pro 990 His Asp 1005 Ala 735 Tyr Val1 Pro Trp Lys 815 Tyr Gly Val1 Ser Met 895 Ala Ile Asn Gly Giu 975 His Leu 720 Gin Phe Ala Giu Arg 800 Leu Thr Leu Giu Tyr 880 Asn Arg Lys Asn Giu 960 Val1 His Lys Asn Ala Asn Pro Arg Ala Arg Ilie His Val Lys Leu Val Ala Giu Gin 1010 1015 1020 WO 01/00843 PCT/IB00/00923 Gly Val Gly Thr Val Ala Ala Gly Val Ser Lys Ala His Ala Asp Val 1025 1030 1035 1040 Val Leu Ile Ser Gly His Asp Gly Gly Thr Gly Ala Ser Pro Leu Thr 1045 1050 1055 Ser Leu Lys His Ala Gly Gly Pro Trp Glu Leu Gly Leu Ala Glu Thr 1060 1065 1070 Gin Gin Thr Leu Leu Leu Asn Gly Leu Arg Asp Arg Ile Arg Val Gin 1075 1080 1085 Cys Asp Gly Gin Leu Lys Thr Gly Arg Asp Val Val Ile Ala Ala Leu 1090 1095 1100 Leu Gly Ala Glu Glu Phe Gly Phe Ala Thr Ala Pro Leu Val Val Glu 1105 1110 1115 1120 Gly Cys Ile Met Met Arg Val Cys His Leu Asp Thr Cys Pro Val Gly 1125 1130 1135 Ile Ala Thr Gin Asn Pro Asp Leu Arg Ser Lys Phe Thr Gly Lys Ala 1140 1145 1150 Glu His Val Val Asn Phe Phe Thr Phe Ile Ala Gin Glu Val Arg Glu 1155 1160 1165 Tyr Leu Ala Gin Leu Gly Phe Arg Ser Ile Asp Glu Ala Val Gly Gin 1170 1175 1180 Ala Gin Val Leu Arg Lys Arg Ser Gly Ile Pro Ala Asp Ser Arg Ala 1185 1190 1195 1200 Ala His Leu Asp Leu Ser Pro Ile Phe His Arg Pro Glu Thr Pro His 1205 1210 1215 Phe Pro Thr Gin Asp Val Arg Cys Thr Lys Thr Gin Glu His Ser Leu 1220 1225 1230 Glu Lys Ala Leu Asp Asn Ala Phe Ile Asp Lys Ala Ser Asp Thr Ile 1235 1240 1245 Thr Arg Ala Ala Ala Gly Val Glu Thr Ser Ile Val Ile Asp Ser Ser 1250 1255 1260 Ile Ser Asn Val Asn Arg Ser Val Gly Thr Met Leu Gly Ser Ala Val 1265 1270 1275 1280 Ser Arg Val Ala Gly Ala Gin Gly Leu Pro Asp Gly Thr Ile Thr Leu 1285 1290 1295 Asn Leu Gin Gly Cys Ala Gly Asn Ser Phe Gly Ala Phe Ile Pro Arg 1300 1305 1310 Gly Ile Thr Ile Asn Leu Thr Gly Asp Ala Asn Asp Phe Val Gly Lys 1315 1320 1325 Gly Leu Ser Gly Gly Lys Ile Val Ile Lys Pro Ser Ala Gin Ala Pro 1330 1335 1340 Lys Gin Leu Lys Asn Asn Pro Asn Ile Ile Ala Gly Asn Val Leu Gly 112 WO 01/00843 WO 0100843PCT/IBOO/00923 1345 1350 1355 1360 Tyr Gly Ala Thr Ser Gly Giu Leu PheIle Arg Gly Gin Val Gly Giu 1365 1370 1375 Arg Phe Cys Val Arg Asn Ser Gly Ala Thr Ala Val Val Giu Gly Ile 1380 1385 1390 Gly Asn His Gly Cys Giu Tyr Met Thr Gly Gly Arg Val Leu Val Leu 1395 1400 1405 Gly Pro Val Gly Giu Asn Phe Gly Ala Gly Met Ser Gly Gly Ile Ala 1410 1415 1420 Tyr Leu Ala Asn Ser Pro Asp Leu Asn Gin Lys Ile Asn Giy Glu Leu 1425 1430 1435 1440 Val Asp Vai Val Pro Leu Ser Ala Asp Asp Leu Thr Trp Ala Asp Giu 1445 1450 1455 Leu Ilie Ala Arg His Arg Giu Leu Thr Gly Ser Giu Thr Lys Leu Arg 1460 1465 1470 Ala Gin Asp Leu Val Lys Ile Met Pro Arg Asp Phe Gin Lys Val Leu 1475 1480 1485 Asn Ile Ilie Giu Thr Ala His Ala Giu Gly Gin Asp Pro Ala Ile Lys 1490 1495 1500 Ile Met Giu Ala Val Ser 1505 1510 <210> 73 <211> 1906 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS <222> (101)..(1906) <223> FRXA00007 <400> 73 atcgaaagta acccttttgt tacttgcgtt gcaggtagtg tccctgattt tcttattatc 6( gaacgattga tagaaacagg attaaagtga ggtatcccgc atg aaa cca caa gga 1: 0 ctc tac aac cct gcg cat gaa cat gac gcc tgc Leu Tyr Asn Pro Ala His Giu His Asp Ala Cys 15 gcg gat atc cac ggt cga ccc agc cgc agc att Ala Asp Ile His Giy Arg Pro Ser Arg Ser Ilie 30 gag gcg ctt cgc aac att gac cac cga ggt gcc Giu Ala Leu Arg Asn Ile Asp His Arg Gly Ala 45 Met Lys Pro Gin Gly 1 ggt gtg gcg ttt att 163 Gly Val Ala Phe Ile gtt gat cgt gca ctt 211 Val Asp Arg Ala Leu gcc ggt gca gag aag 259 Ala Gly Ala Giu Lys WO 01/00843 WO 0100843PCT/EBOO/00923 aac act ggc gat ggt gcg ggc atc ctc atg cag att ccg gac ggc ttt 307 Asn Thr Gly Asp Gly Ala Gly Ile Leu Met Gin Ile Pro Asp Gly Phe 60 tat cgt gaa gta tct ggc att gag ctt cct gag gca ggg gag tat gcc 355 Tyr Arg Giu Val Ser Gly Ilie Giu Leu Pro Giu Ala Gly Giu Tyr Ala 75 80 act ggt att. gcg ttc ttg cct cgc ggt cgc atg gcg atg atg gat gct 403 Thr Giy Ile Ala Phe Leu Pro Arg Gly Arg Met Ala Met Met Asp Ala 95 100 cag aag gaa att gag cgc atc gca aag caa gaa ggt gcc gat gtg ctt 451 Gin Lys Giu Ile Giu Arg Ilie Ala Lys Gin Giu Gly Ala Asp Val Leu 105 110 115 ggt tgg cgc atg gtt cct ttt gat tct cgt gat ttg ggt tcc atg gct 499 Gly Trp Arg Met Vai Pro Phe Asp Ser Arg Asp Leu Gly Ser Met Ala 120 125 130 gag gag gcg atg cct agt ttc gcg cag att ttc ctt act gtg cct gga 547 Giu Giu Ala Met Pro Ser Phe Aia Gin Ile Phe Leu Thr Val Pro Gly 135 140 145 aaa tct ggt gaa gat ctt gac cgt gtg atg ttc ttt atc cgt aag cgt 595 Lys Ser Giy Giu Asp Leu Asp Arg Val Met Phe Phe Ile Arg Lys Arg 155 160 165 tgt gag cgt gag ctg ggc acc acc aat ggt cgc gat acg gtg tat ttc 643 Cys Giu Arg Giu Leu Gly Thr Thr Asn Gly Arg Asp Thr Val Tyr Phe 170 175 180 ccg tcg cta tct tca cgc acc atc att tac aaa ggc atg ttg acc act 691 Pro Ser Leu Ser Ser Arg Thr Ile Ile Tyr Lys Giy Met Leu Thr Thr 185 190 195 ctg cag ctt gag ggc ttc ttt gag gat ctg ggt gat gct cgc ctg gag 739 Leu Gin Leu Giu Giy Phe Phe Giu Asp Leu Gly Asp Ala Arg Leu Giu 200 205 210 tcg gcc att gct att gtg cac tcg cgt ttc tcc acg aac act ttc cca 787 Ser Ala Ilie Ala Ilie Val His Ser Arg Phe Ser Thr Asn Thr Phe Pro 215 220 225 agc tgg ccg ctg gcg cac ccg tac cgt ttc gtt gcc cac aac ggt gag 835 Ser Trp Pro Leu Ala His Pro Tyr Arg Phe Val Ala His Asn Gly Glu 230 235 240 245 atc aac act gtg cgt ggc aat gaa aac tgg atg cgc gcc cgc gag gcg 883 Ile Asn Thr Val Arg Gly Asn Giu Asn Trp Met Arg Ala Arg Giu Ala 250 255 260 ctt atc aaa aac gac aag ctg ggc aat ttg agc agc gtg ctg cct atc 931 Leu Ilie Lys Asn Asp Lys Leu Gly Asn Leu Ser Ser Val Leu Pro Ile 265 270 275 tgc acc ccg gag ggc tcg gat acc gcg cgt ttc gac gag gct ttg gag 979 Cys Thr Pro Giu Gly Ser Asp Thr Ala Arg Phe Asp Glu Ala Leu Giu 280 285 290 WO 01/00843 WO 0100843PCTIBOOIOO923 ctt ttg 1027 Leu Leu 295 atc cct 1075 Ile Pro 310 gat ttc 1123 Asp Phe cac ctg ggc gga His Leu Gly Gly cag gcg tgg gaa.

Gin Ala Trp Giu 315 tac Tyr 300 c ac His tca ctt ccg cat gct gtt gcg atg atg Ser Leu Pro His Ala Val Ala Met Met 305 aac aag acg ctg agc cct gag ctg cgt Asn Lys Thr Leu Ser Pro Glu Leu Arg tac gaa tac Tyr*Giu Tyr 330 .320 tct tgt ctg atg gag Ser Cys Leu Met Giu 335 325 cca tgg gat ggt cct Pro Trp Asp Gly Pro 340 gca gcg ctg gca ttt 1171 Ala Ala Leu Ala Phe 345 cgt aat ggc ctg cga 1219 Arg Asn Gly Leu Arg 360 gtt gtg atg gct tct 1267 Val Val Met Ala Ser 375 gtc gta aag cgt act 1315 Val Val Lys Arg Thr 390 act gcc gag ggc cgc 1363 Thr Ala Giu Giy Arg 410 agc gaa gcg cag cca 1411 Ser Giu Ala Gin Pro 425 ctg gat cgt ctg cct 1459 Leu Asp Arg Leu Pro 440 gct gtg ttg cgt cag 1507 Ala Vai Leu Arg Gin 455 ttg ttg ctg ctg ccg 1555 Leu Leu Leu Leu Pro 470 act Thr gac ggt cgt Asp Gly Arg 350 cct ggg Pro Gly gaa tcg Giu Ser 380 cgc gta Arg Val 395 cga Arg 365 atc Ile ttt gtg ggt gcc gtg ctg gac Phe Vai Gly Ala Val Leu Asp 355 acc att act gat tcg ggt ttg Thr Ile Thr Asp Ser Gly Leu 370 ttg gac ttg agg gag gag agc Leu Asp Leu Arg Glu Giu Ser gga gtg Gly Val cag cct gga cgc Gin Pro Gly Arg 400 385 atg ttc ctt. gtt gac Met Phe Leu Val Asp 405 atc aag cag aaa tta Ile Lys Gin Lys Leu 420 atc gtt gaa gac Ile Vai Glu Asp tat ggt gag tgg Tyr Gly Glu Trp 430 cag aca cgc tac Gin Thr Arg Tyr gag Giu 415 gaa Glu att cgc gat aat ttt gtg cat Ile Arg Asp Asn Phe Val His 435 aac tac atg gcg cac tct cgt Asn Tyr Met Ala His Ser Arg 450 atc act gaa gaa gat gtg gat Ile Thr Glu Glu Asp Val Asp 445 cgt gtt ttc gga Arg Val Phe Gly 460 465 atg gcc cgc cag ggt gct gag Met Ala Arg Gin Gly Ala Glu 475 480 gcg att ggt tcc Ala Ile Gly Ser 485 WO 01/00843 atg ggt tcg gat acg cca att gcg5 1603 Met Gly Ser Asp Thr Pro Ile Ala i 490 ctt tat gat ttc ttc gcg cag cgc 1 1651 Leu Tyr Asp Phe Phe Ala Gin Arg 1 505 ttg gac tct atc cgc gaa aag cctc 1699 Leu Asp Ser Ilie Arg Giu Lys Pro 1 520 525 ggt gcg cag tct gac gtg ctc aat 1747 Gly Ala Gin Ser Asp Val Leu Asn 1 535 540 att cgt ttg gaa tcg ccg atc attc 1795 Ilie Arg Leu Giu Ser Pro Ilie Ile 550 555 atc aat gcc aac gcg cat ggt gag 1 1843 Ile Asn Ala Asn Aia His Gly Glu 570 att tct ggt ttg tac cca gtg gct 1891 Ilie Ser Gly Leu Tyr Pro Val Ala 1 585 gcg att gct cgt gtg 1906 Ala Ile Ala Arg Val 600 <210> 74 <211> 602 <212> PRT <213> Corynebacterium giutaxnicum <400> 74 Met Lys Pro Gin Gly Leu Tyr AsnI 1 5 Giy Vai Ala Phe Ile Ala Asp Ile 1 Vai Asp Arg Ala Leu Glu Aia Leu 40 Ala Gly Ala Glu Lys Asn Thr Gly 55 Ile Pro Asp Gly Phe Tyr Arg Giu 70 cta Leu 495 gc t Ala acc Thr gg t Giy aac Asn ga t Asp 575 cat His Ala 10 Gly Asn Gly Ser PCT/iBOOIOO923 cgc atg Arg Met 500 cca ccg Pro Pro ttg ttg Leu Leu cga cgt Arg Arg acc ttg Thr Leu 565 gct gta Ala Vai 580 aag gct Lys Ala Giu His Asp Aia Cys Pro Ser Arg Ser Ile Asp His Arg Gly Aia Giy Ile Leu Met Gin Ile Giu Leu Pro Giu WO 01/00843 WO 0100843PCTIIBOO/00923 Ala Ala Gly Leu Leu 145 Phe Asp Gly Asp Thr 225 Ala Arg Ser Asp Ala 305 Ser Pro Gly Thr Leu 385 Gly Met Ala Gly 130 Thr Ile Thr Met Ala 210 Asn His Ala Val1 Glu 290 Val Pro Trp Ala Asp 370 Arg Tyr Asp 100 Val Met Pro Lys Tyr 180 Thr Leu Phe Gly Giu 260 Pro Leu Met Leu Gly 340 Leu G ly Glu Al a Al a Leu Ala Gly Arg 165 Phe Thr Giu Pro Glu 245 Ala Ile Giu Met Arg 325 Pro Asp Leu Ser I le Glu Arg 120 Ala Gly Arg Leu Leu 200 Ile Pro Thr Lys Pro 280 His Gin Tyr Leu Gly 360 Met Lys Ala Ile 105 Met Met Giu Giu Ser 185 Glu Ala Leu Val1 Asn 265 Glu Leu Al a Glu Al a 345 Leu Ala Arg Phe 90 Glu Val1 Pro Asp Leu 170 Ser Gly Ile Al a Arg 250 Asp Gly Gly Trp Tyr 330 Phe Arg Ser Thr Leu Arg Pro Ser Leu 155 Gly Arg Phe Val1 His 235 G ly Lys Ser Gly Glu 315 His Thr Pro Giu Arg 395 Pro I le Phe Phe 140 Asp Thr Thr Phe His 220 Pro Asn Leu Asp Tyr 300 His Ser Asp Gly Ser 380 Val1 Arg Gin Arg Ile Met Gly 175 Tyr Leu Phe Phe Trp, 255 Leu Arg Pro Thr Met 335 Phe Thr Leu Gly Met Giu Asp Phe Phe 160 Arg Lys Gly Ser Val1 240 Met Ser Phe His Leu 320 Glu Val1 Ile Asp Arg 400 WO 01/00843 Met Phe Leu Val Asp 405 PCTIIBOO/00923 Ile Vai Giu Asp Giu Giu 415 Thr Ala Giu Gly Ile Asp Met Giu 465 Giu Gin Val1 Met Asp 545 Giu Phe Ala Gin Phe 435 His Asp Ile Pro Asn Thr Aia Ala Ala Met 595 Lys 420 Val1 Ser Val1 Gly Arg 500 Pro Leu Arg Thr Aia 580 Lys Leu His Arg Asp Ser 485 Met Pro Leu Arg Leu 565 Val1 Ala Giu Asp Val1 455 Leu Giy Tyr Asp Ala 535 Arg Asn Ser Ile Gin 425 Leu Arg Leu Asp Phe 505 Ile Ser Giu Asn Leu 585 Arg Arg 410 Pro Pro Gin Pro Thr 490 Phe Arg Asp Ser Ala 570 Tyr Val1 Tyr Gin Arg Met 475 Pro Ala Giu Val Pro 555 His Pro Gly Thr Val1 460 Aia Ile Gin Lys Leu 540 Ile Gly Val1 Trp 430 Tyr Gly Gin Ala Phe 510 Val1 Pro Asp Trp His 590 Arg Tyr Thr Ala 480 Ser Gin Ser Pro His 560 Ser Gly <210> <211> 1362 <212> DNA <213> Corynebacteriun giutaiicun <22 0> <221> CDS <222> (70)..(1362) <223> FRXA00364 <400> accaatttct ggcatcggtc tggatgaagt tgcagctgac gtagaaagct cgtcaccgca gcgcattttttg cca cgc cct gaa gag cac gct cac cgt gaa ttg gat ttg 111 Leu Pro Arg Pro Giu Giu His Ala His Arg Giu Leu Asp Leu 1 5 ggt ggt gaa tac aag tgg cgc cgc gaa ggt gaa tac cac ctg ttc aac 159 Gly Gly Giu Tyr Lys Trp Arg Arg Giu Gly Giu Tyr His Leu Phe Asn 20 25 WO 01/00843 cca gaa acc Pro Giu Thr gag att ttc Giu Ile Phe ttg ggt act Leu Giy Thr att tcg gtg Ile Ser Val tcc act ggt Ser Thr Gly gtc ttg gcc Val Leu Ala gaa ggt ggc Giu Gly Gly tgg aag cgc Trp Lys Arg 145 acc agc cac Thr Ser His 160 cag ggc gca Gin Gly Ala 175 tac cca tgg Tyr Pro Trp att tcc cct Ile Ser Pro cag ctg atc Gin Leu Ile 225 gtg aag cta Vai Lys Leu 240 tcc aaa gca Ser Lys Ala 255 act ggc gca ttC Phe gat Asp cgt Arg gag Giu atg Met gcc Ala 115 gac

ASP

gcc Ala t tg Leu ccc Pro gca Ala 195 c ca Pro gac Asp gca Ala gc t Ala cct aag Lys tac Tyr gga Gly gtg Val1 tc t Ser 100 atg Met gcc Ala att Ile aac Asn gg t Gly 180 gaa Giu cac His ctg Leu gaa Glu ga t

ASP

260 t tg ctg Leu acc Thr ctg Leu gag Giu 85 tat Tyr aac Asn cgc Arg aag Lys aac Asn 165 gaa Giu gtc Val cac His aag Lys caa Gin 245 gtg Val1 acc cag Gin cgc Arg ttt Phe 70 ccg Pro ggc Gly cga Arg cga Arg cag Gin 150 tgc Cys ggt Gly cgc Arg gat Asp aac Asn 230 ggc G ly gtg Val tcc cat His aag Lys gag Giu gtc Val1 tcg Ser ctg Leu ttt Phe 135 gtg Val acc Thr ggc Gly atc Ile at t Ile 215 gc t Ala gtg Val1 ctt Leu c tg gca Al a 40 gt t Val1 t tc Phe agt Ser att Ile ggc Gly 120 ga t Asp gcc Ala gat Asp cag Gin ac c Thr 200 tac Tyr aac Asn ggc Gly att Ile aag acg Thr gat Asp agc Ser gag Glu tc t Ser 105 gg t Gly gtg Val1 tcg Ser att Ile ctg Leu 185 ac c Thr tcc Ser cca Pro acc Thr tcc Ser 265 cat cgt Arg ga t Asp acg Thr atc Ile gc t Ala atg Met gaa Giu gga Gly cag Gin 170 cca Pro cca Pro att Ile cgc Arg gtt Val1 250 ggc Gly gcc tct Ser caa Gin gac Asp gtg Val1 gaa Glu tcc Ser ccc Pro cgt Arg 155 atc Ile cca Pro ggc Gly gag Giu gc a Ala 235 gcc Ala cac His ggt ggc Gly tcc Ser cgc Arg aag Lys gcc Ala aac Asn aac Asn 140 ttc Phe aag Lys aac Asn gtt Val1 ga t Asp 220 cga Arg gca Ala gat Asp ggt PCTflBOO/00923 agc tac 207 Ser Tyr act cgc 255 Thr Arg aag cca 303 Lys Pro cgt ttc 351 Arg Phe cat gag 399 His Giu 110 tcc ggc 447 Ser Gly 125 ggt gac 495 Gly Asp ggc gtg 543 Gly Val atg gca 591 Met Ala aag gtg 639 Lys Val 190 ggt ctg 687 Gly Leu 205 ctg gct 735 Leu Ala atc cac 783 Ile His ggt gtg 831 Gly Val ggc gga 879 Gly Gly 270 cca tgg 927 WO 01/00843 WO 0100843PCTJIBOO/00923 Thr Gly Ala Ser Pro 275 gc t Al a Leu Thr Ser Leu Lys 280 acg Thr His Ala Gly Giy Pro Trp, 285 gag ttg ggc Glu Leu Gly gaa acc cag Giu Thr Gin ttg ctg ctc Leu Leu Leu aac ggc ctg Asn Gly, Leu 300 cgc gat 1023 Arg Asp gac gtg 107.1 Asp Val 320 acc gca 1119 Thr Ala 335 ctg gac 1167 Leu Asp tcc aag 1215 Ser Lys atc gcc 1263 Ile Ala att gat 1311 Ile Asp 400 atc cca 1359 Ile Pro 415 atc 1362 Ile cgc gtg cag tgc Arg Val Gin Cys 310 ggt cag ctg aaa act ggc cga Gly Gin Leu Lys Thr Gly Arg 315 gtt atc gca gct ctt Val Ile Ala Ala Leu 325 ccg ctg gtg gtt gaa Pro Leu Val Val Glu 340 acc tgc ccg gtg ggt Thr Cys Pro Val Gly 355 ttc acc ggc aag gct Phe Thr Gly Lys Ala ctc ggt gcc gaa Leu Gly Ala Glu ggc tgc atc atg Gly Cys Ile Met 345 atc gct acc cag Ile Ala Thr Gin gaa Glu 330 ttc ggt ttt gcc Phe Gly Phe Ala atg cgc gtc tgc cac Met Arg Val Cys His 350 aac ccg gat ttg cgt Asn Pro Asp Leu Arg 365 aac ttc ttc acc ttc Asn Phe Phe Thr Phe 370 cag gaa Gin Glu gtc cgt gag Val Arg Giu gaa cac Glu His 375 tac ttg Tyr Leu 390 360 gtg gtc Val Val 380 gca cag ctt Ala Gin Leu gaa gcc gtc gga caa Glu Ala Val Gly Gin 405 gct gat tcc cgc gca Ala Asp Ser Arg Ala 420 gcc cag gtg ctg cgc Ala Gln Val Leu Arg 410 gca cac ctg gat ttg Ala His Leu Asp Leu 425 ggt ttc cgc tct Gly Phe Arg Ser 395 aag cgt tcc gga Lys Arg Ser Gly agc cca. att ttc Ser Pro Ile Phe 430 <210> 76 <211> 431 <212> PRT <213> Corynebacterium giutarnicum <400> 76 Leu Pro Arg Pro Giu Glu His Ala His Arg Glu Leu Asp Leu Gly Gly 1 5 10 WO 01/00843 WO 0100843PCTIiBOO/00923 Giu Thr Phe Thr Val1 Gly Ala Gly Arg 145 His Ala Trp Pro Ile 225 Leu Ala Ala Gly Arg 305 Val1 Tyr Ile Lys Ile Ser Ala Ile Glu 130 Ser Tyr Lys Val1 Pro 210 His Val1 His Ser Leu 290 Ile Ile Lys Phe Asp Arg Giu Met Al a 115 Asp Ala Leu Pro Al a 195 Pro Asp Ala Ala Pro 275 Ala Arg Ala Trp Lys Tyr Gly Val Ser 100 Met Ala Ile Asn Gly 180 Giu His Leu Giu Asp 260 Leu Giu Val1 Al a Arg Leu Thr Leu Giu Tyr Asn Arg

LYS

Asn 165 Giu Val His Lys Gin 245 Val Thr Thr Gin Leu 325 Tyr Arg Asp Thr Ile 90 Ala Met Glu Gly Gin 170 Pro Pro Ile Arg Val1 250 Gly Ala Leu Leu Giu 330 His Ser Gin Asp Val1 Glu Ser Pro Arg 155 Ile Pro Gly Giu Ala 235 Ala His Gly Leu Lys 315 Phe Pro Glu Leu Ile Ser Val1 Giu Trp, Thr Gin 175 Tyr Ile Gin Val1 Ser 255 Thr Glu Arg Asp Thr 335 WO 01/00843 Pro Leu Val Thr Cys Pro 355 Phe Thr Gly 370 Gin Glu Val 385 Glu Ala Val Ala Asp Ser Glu Gly Ala Glu Gin 405 Ala Val Asp Phe 380 Phe Arg Pro PCTIBOO/00923 Leu Asp Ser Lys Ile Ala Ile Asp 400 Ile Pro 415 Ile <210> 77 <211> 866 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> <223> FRXA00367 <400> 77 aaa Lys cg t Arg agc Ser cgc Arg ctt Leu atc Ile tta Leu cag gcc Ala gcc Ala aac Asn gtg Val1 caa Gin acc Thr tct Ser ctg gat Asp agc Ser acg Thr cca Pro ttt Phe gcc Ala aag Lys att Ile 125 aag Lays att Ile atg Met gac Asp ggc Gly aat Asn cc t Pro 110 gcc Ala Gin Ala Pro Lys Gin Leu Lys Asn Asn WO 01/00843 WO 0100843PCTIiBOO/00923 gtg ctt gga tac ggc Val Leu Gly Tyr Gly 130 gca acc agt ggt gaa ttg ttc att cgt Ala*Thr Ser Gly Glu Leu Phe Ile Arg 135 140 gtc Val 145 gaa Glu ctg Leu ggc Gly ggC G ly gct Ala 225 aag Lys aaa Lys gaa Glu a tc Ilie ttg Leu gca Ala 195 ttg Leu gag Glu cgt Arg ctc Leu cgt ttc Arg Phe gga aac Gly Asn 165 ggc ccg Gly Pro 180 tac ctg Tyr Leu gtg gat Val Asp ctc att Leu Ilie gca caa Ala Gin 245 aac atc AsnIlie 260 tgc Cys 150 cac His gtt Val1 gct Ala gtt Val gc t Ala 230 ga t Asp atc Ile gtc Val1 ggt Gly gg t Gly aa t Asn gtt Val1 215 cgc Arg ttg Leu gaa Glu gca Ala ggC Gly atg Met 190 aag Lys ctg Leu tcc Ser ga t Asp caa ggC Gly gtg Val1 cga Arg 175 tc t Ser atc Ile acg Thr gag Giu ttc Phe 255 gac His Ala Giu Gly Gin Asp Pro gca atc aag atc atg gag gc-a gtg agc taatggccga cccacaagga Ala Ile Lys Ile Met Glu Ala Val Ser 275 280 t tc <210> 78 <211> 281 <212> PRT <213> Corynebacterium giutamicum <400> 78 His Ser Leu Glu Lys Ala Leu Asp Asn Ala Phe Ile Asp Lys Ala Ser 1 5 10 Asp Thr Ile Thr Arg Ala Ala Ala Gly Val Giu Thr Ser Ile Val Ile 25 Asp Ser Ser Ile Ser Asn Val Asn Arg Ser Val Gly Thr Met Leu Gly 40 Ser Ala Val Ser Arg Val Ala Gly Ala Gin Gly Leu Pro Asp Gly Thr 55 WO 01/00843 WO 0100843PCT/EBOO/00923 Ile Thr Leu Asn Leu Gin Gly Cys Ala Gly Asn Ser Phe Gly Ala Phe, 70 75 Ilie Pro Arg Gly Ile Thr Ile Asn Leu Thr Gly Asp Ala Asn Asp Phe 90 Val Gly Lys Gly Leu Ser Gly Gly Lys Ile Val Ile Lys Pro Ser Ala 100 105 110 Gin Ala Pro Lys Gin Leu Lys Asn Asn Pro Asn Ile Ile Ala Gly Asn 115 120 125 Vai Leu Gly Tyr Gly Ala Thr Ser Gly Giu Leu Phe Ile Arg Gly Gin 130 135 140 Val Gly Giu Arg Phe Cys Val Arg Asn Ser Giy Ala Thr Ala Val Val 145 150 155 160 Giu Gly Ilie Gly Asn His Gly Cys Giu Tyr Met Thr Gly Gly Arg Val 165 170 175 Leu Val Leu Gly Pro Val Gly Giu Asn Phe Gly Ala Gly Met Ser Gly 180 185 190 Giy Ile Ala Tyr Leu Ala Asn Ser Pro Asp Leu Asn Gin Lys Ile Asn 195 200 205 Gly Giu Leu Vai Asp Val Vai Pro Leu Ser Ala Asp Asp Leu Thr Trp 210 215 220 Ala Asp Giu Leu Ile Aia Arg His Arg Giu Leu Thr Giy Ser Giu Thr 225 230 235 240 Lys Leu Arg Ala Gin Asp Leu Val Lys Ile Met Pro Arg Asp Phe Gin 245 250 255 Lys Val Leu Asn Ilie Ilie Giu Thr Ala His Ala Giu Gly Gin Asp Pro 260 265 270 Ala Ile Lys Ile Met Giu Ala Val Ser 275 280 <210> 79 <211> 1494 <212> DNA <213> Corynebacteriumn giutamicun <220> <221> CDS <222> (101)..(1471) <223> RXN00076 <400> 79 tctaggagtg ttaaacagcc tggacttgaa acacctttaa ctacttgatt ttcacaccct tgtttccata aaagggctca cgaaaggcaa cttcaaacac atg aca act ccc ctg 115 Met Thr Thr Pro Leu 1 WO 01/00843 WO 0100843PCT/1BOO/00923 cgc Arg ctc Leu caa Gin cac His aag Lys acc Thr ggC Gly ggc Gly cgc Arg ggc Giy 150 ggC Gly C tc Leu cca Pro tcc Ser ggc Gly 230 gta Val C tc Leu atg Met cca Pro cca Pro gtc Val1 gca Ala t cc Ser ttc Phe 135 gtt Val gac Asp aaa Lys gca Ala

CCC

Pro 215 gcc Ala gcc gtc atc gga gct ggc cct gct ggc Ala Val Ile Gly Ala Gly Pro Ala Gly 15 atc cgc aat gaa gag cgc gaa gtg ttc Ile Arg Asn Giu Giu Arg Giu Val Phe 30 cct gca ccg ttc gga ctc atc cgt tac Pro Ala Pro Phe Gly Leu Ile Arg Tyr cgc atc aag ggc atc gtt aag tcc ctg Arg Ile Lys Gly Ile Val Lys Ser Leu cgc ctg cgc ctg CtC ggt aac att gaa Arg Leu Arg Leu Leu Gly Asn Ile Glu 75 gaa gaa ctc cgc gac tac tac gat gca Giu Glu Leu Arg Asp Tyr Tyr Asp Ala 95 gtt gca gac cgc gac ctc aac atc ccc Val Ala Asp Arg Asp Leu Asn Ile Pro 105 110 ttc ggt gcc ggc gag ttc gtt ggc ttc Phe Gly Ala Gly Giu Phe Val Gly Phe 120 125 gag cgc tcc tgg gat ctg tct gca cag Glu Arg Ser Trp Asp Leu Ser Ala Gin 140 ggt aac gtc ggc ctc gac gta gcc cgc Gly Asn Val Gly Leu Asp Val Ala Arg 155 160 gag ctc aaa gtc acc gaa att tcc gac Glu Leu Lys Val Thr Giu Ile Ser Asp 170 175 gaa aac aag gcc act gaa gtg cac gtt Glu Asn Lys Ala Thr Giu Val His Val 185 190 cag gtc aag ttc acc cca cag gaa ctc Gin Val Lys Phe Thr Pro Gin Giu Leu 200 205 acc atc aac gtg gtt gtt gat cca gaa Thr Ilie Asn Val Val Val Asp Pro Giu 220 tct gaa gaa gcc cgc cgc gca tcc aag Ser Glu Glu Ala Arg Arg Ala Ser Lys 235 240 att tac gca Ile Tyr Ala ctt Leu gc t Ala gtg Val1 aaa

LYS

ttc Phe gaa Giu 115 ggc Gly gct Ala gc t Ala tac Tyr cgt Arg 195 c tc Leu gac Asp gac Asp tc Ser t tc Phe cca Pro t tg Leu gac Asp tCC Ser 100 gca Ala aac Asn gtt Val aag Lys gac Asp 180 cgt Arg gac Asp tac Tyr c tg Leu aac 163 211 259 307 355 403 451 499 547 595 643 691 739 787 835 tgc cag atc ctg gaa cag tac gca atc cgc gag cca aag gac gcE ccg WO 01/00843 Cys Gin Ile cac acc ctg His Thr Leu aag gac ggc Lys Asp Gly 280 ggc aac ggc 1027 Giy Asn Giy 295 cag gct gtc 1075 Gin Ala Val 310 gtc cca ttc 1123 Val Pro Phe gtc ctc acc 1171 Vai Leu Thr ggc tgg atc 1219 Gly Trp Ilie 360 gac gcc aag 1267 Asp Ala Lys 375 gta ctt gaa 1315 Vai Leu Glu 390 ctc gat tcc 1363 Leu Asp Ser ctc gac gca 1411 Leu Asp Ala aag aag att 1459 Lys Lys Ile 440 Leu cag Gin 265 aag Lys ggC Gly tac Tyr gat Asp gct Ala 345 aag Lys gaa Giu gc t Ala cgc Arg gca Al a 425 gtt Val1 Giu 250 atc Ile gtt Val gta Val1 cgc Arg gag Giu 330 cca Pro cgt Arg acc Thr cca Pro aac Asn 410 gag Glu gat Asp Ala ttt Phe ctg Leu 285 acc Thr ggC Gly c ac His gaa Glu atc Ile 365 atc Ile cag Gin ttc Phe ctc Leu gaa Glu 445 Ile gaa Giu 270 cgc Arg ggc Gly t ac Tyr gtC Val cca Pro 350 ggt Gly c tc Leu ggC Gly acc Thr ggt Gly 430 atg Met Arg 255 aac Asn ac c Thr gaa Glu aag Lys atc Ile 335 gta Val cta Leu atc Ile gaa Glu acc Thr 415 gaa Glu gtc Val1 Giu cca Pro gaa Glu ttc Phe tcc Ser 320 cc t Pro cca Pro atc Ile aag Lys gaa Giu 400 tgg Trp gcc Ala cgc Arg Pro gtt Val1 cgc Arg aag Lys 305 gac Asp aat Asn ggc Gly ggc Giy ga t Asp 385 gcc Ala gaa Giu gaa Glu cag Gin Lys gag Giu acc Thr 290 gac Asp ccc Pro gac

ASP

c tc Leu aac Asn 370 gcc Ala atc Ile ggc G ly ggc Gly gcc Al a 450 PCTIIBOO/00923 Ala Pro 260 ctt caa 931 Leu Gin ctt gat 979 Leu ASP cca gtc Pro Val gac ggc Asp Gly 325 gga cat Gly His 340 gca acc Ala Thr aag tcc Lys Ser gcc ggt Ala Giy gag ctt Glu Leu 405 tac aaa Tyr Lys 420 gag cgc Giu Arg gaa gct Giu Ala WO 01/00843 WO 0100843PCTJ/iBOO/00923 cca gca att gtc taaattgttt taacgcgtga agc 1494 Pro Ala Ile Val 455 <210> <211> 457 <212> PRT <213> Corynebacteriun giutamicun <400> Met Thr Thr Pro Leu Arg Val Ala Vai Tyr Asp Val1 Asn Gly Val1 Ile Asp 130 Val Leu Val Gly Glu 210 Ile Gin Ala Leu Ala Val1 Lys Phe Giu 115 G ly Ala Ala Tyr Arg 195 Leu Asp Asp Ser Phe Pro Leu Asp Ser 100 Ala Asn Val Lys Asp 180 Arg Asp Tyr Leu Asp Glu Asp Asp Ile Thr Glu Pro Ile Thr 165 Ser Gly His Asp Val 245 Ile Pro 40 Arg Arg Glu Val1 Phe 120 Glu Gly G iu Giu Gin 200 Thr Ser Ile Arg 25 Ala Ile Leu Glu Ala 105 Giy Arg Asn Leu Asn 185 Val1 Ile Glu Leu Ile 10 Asn Pro Lys Arg Leu 90 Asp Aia Ser Val1 Lys 170 Lys Lys Asn Giu Giu 250 Gly Glu Phe Gly Leu Arg Arg Gly Trp Giy 155 Val Ala Phe Vai Al a 235 Gln Ala Glu Gly Ile Leu Asp Asp Glu

ASP

140 Leu Thr Thr Thr Val1 220 Arg Tyr Gly Arg Leu Val1 G ly Tyr Leu Phe 125 Leu

ASP

G iu Giu Pro 205 Val1 Arg Ala Pro Giu Ile Lys Asn Tyr Asn 110 Val1 Ser Val1 Ile Val1 190 Gin Asp Ala Ile Ala Val1 Arg Ser Ile Asp Ile G ly Ala Ala Ser 175 His Glu Pro Ser Arg 255 Giy Phe Tyr Leu Glu Ala Pro Phe Gln Arg 160 Asp Vai Leu Giu Lys 240 Glu Pro Lys Asp Ala Pro His Thr Leu Gin Ile His Leu Phe Glu Asn Pro WO 01/00843 WO 0100843PCTflIBOOIOO923 Val1 Arg Lys 305 Asp Asn Gly Gly Asp 385 Ala Giu Giu Gin Glu Thr 290 Asp Pro Asp Leu Asn 370 Al a Ile Gly Gly Ala 450 Leu Gin Lys Asp Gly Lys Val Val Gly Leu Arg Thr Glu G ly Gin 310 Val1 Val1 Gly Asp Val1 390 Leu Leu Lys Pro Asn 295 Ala Pro Leu Trp Ala 375 Leu Asp

ASP

Lys Ala 455 280 Gly Gly Val Tyr Phe Asp Thr Ala 345 Ile Lys 360 Lys Giu Glu Ala Ser Arg Ala Ala 425 Ile Val 440 Ile Val 285 Thr Gly His Glu Ile 365 Ile Gin Phe Leu Gly Tyr Val1 Pro 350 Gly Leu Gly Thr Gly 430 Giu Lys Ile 335 Vali Leu Ile Giu Thr 415 Glu Phe Ser 320 Pro Pro Ile Lys Giu 400 Trp Ala Trp, Giu Giu Met Vai Arg 81 <211> 786 <212> DNA <213> Corynebacteriun giutamicun <22 0> <221> CDS <222> (101)..(763) <223> FRXA00075 <400> 81 tctaggagtg ttaaacagcc tggacttgaa acacctttaa ctacttgatt ttcacaccct tgtttccata aaagggctca cgaaaggcaa Cttcaaacac atg aca act ccc ctg Met Thr Thr Pro Leu 1 cgc gta gcc gtc atc gga gct ggc cct gct ggc att tac gca tcc gac Arg Val Ala Val Ile Gly Ala Gly Pro Ala Gly Ile Tyr Ala Ser Asp 15 ctc ctc atc cgc aat gaa gag cgc gaa gtg ttc gtt gac ctt ttc gag 128 WO 01/00843 Leu Leu Il caa aig cc Gin Met Pr 4 cac cca cq His Pro Ar aag cca eg Lys Pro Ar acc gte ga Thr Vai GI ggc gca gt Gly Ala Va ggc tee tt Gly Ser P1~ 12 cgc ttc ge Arg Phe GI 135 gge gtt gg Gly Val GI 150 ggc gac ge Gly Asp G) etc aaa ga Leu Lys GI ccc age ac Pro Ser T1 2C Asn ccg Pro aag Lys cgc Arg etc Leu gac Asp gcc Ala tee Ser gtc Val1 aaa Lys 170 aag Lys caa Gin Giu Val Phe Val atc cgt tac ggc Ile Arg Tyr Gly aag tee ctg cac Lys Ser Leu His aac att gaa atc Asn Ile Giu Ile 80 tae gat gca gte Tyr Asp Ala Val 95 aac atc ccc gga Asn Ile Pro Giy 110 gtt ggc ttc tac Vai Giy Phe Tyr tet gca cag tee Ser Ala Gin Ser 145 gta gcc cgc atc Vai Ala Arg Ile 160 att tec gac aac Ile Ser Asp Asn 175 gtg cac gtt tte Vai His Val Phe 190 aca gga act rnaa Thr Gly Thr Xaa

ASP

gtt Vali aac Asn gge Giy gtg Val1 att Ile gac Asp 130 gte Val ctg Leu gte Vai gga Giy aga Arg 210 Leu get Ala gtg Val1 aaa Lys tte Phe gaa Giu 115 gge Giy get Ala get Ala tac Tyr cgt Arg 195 act PCTIBOOIOO923 he Giu ca gac 259 ro Asp tg gac 307 eu Asp ae atc 355 sp Ile cc ace 403 er Thr 00 ca gaa 451 ia Giu ae eca 499 sn Pro tt ate 547 ai Ile ag aca 595 ys Thr 165 ac tee 643 sp Ser gg tgg 69i rg Trp gr eca 739 Thr Xaa Pro etc ccc cac eat caa cgt ggt tgt tgateeagaa gacategact aeg Leu Pro His His Gin Arg Gly Cys 215 220 <210> 82 <2ii> 221 <2i2> PRT <213> Corynebacterium giutaicu <400> 82 Met Thr Thr Pro Leu Arg Val Ala Vai Ile Gly Ala Gly Pro Ala Gly 1 5 10 WO 01/00843 WO 0100843PCTI/iBOO/00923 Ile Tyr Ala Ser Asp Leu Leu Ilie Arg Asn Giu Giu Arg Giu Val Phe 25 Val Asp Leu Phe Giu Gin met Pro Ala Pro Phe Gly Leu Ile Arg Tyr 40 Gly Val Ala Pro Asp His Pro Arg Ile Lys Giy Ile Val Lys Ser Leu 55 His Asn Val Leu Asp Lys Pro Arg Leu Arg Leu Leu Gly Asn Ile Giu 70 75 Ile Giy Lys Asp Ilie Thr Vai Giu Glu Leu Arg Asp Tyr Tyr Asp Ala 90 Val Vai Phe Ser Thr Giy Ala Vai Ala Asp Arg Asp Leu Asn Ile Pro 100 105 110 Gly Ile Giu Ala Giu Gly Ser Phe Gly Ala Gly Giu Phe Val Gly Phe 115 120 125 Tyr Asp Gly Asn Pro Arg Phe Giu Arg Ser Trp Asp Leu Ser Ala Gin 130 135 140 Ser Val Ala Val Ile Giy Val Gly Asn Val Gly Leu Asp Val Ala Arg 145 150 155 160 Ile Leu Ala Lys Thr Gly Asp Glu Leu Lys Val Thr Glu Ile Ser Asp 165 170 175 Asn Val Tyr Asp Ser Leu Lys Giu Asn Lys Xaa Xaa Glu Val His Val 180 185 190 Phe Gly Arg Arg Trp, Pro Ser Thr Gly Gin Val His Pro Thr Gly Thr 195 200 205 Xaa Arg Thr Xaa Pro Leu Pro His His Gin Arg Gly Cys 210 215 220 <210> 83 <211> 672 <212> DNA <213> Corynebacteriun giutanicuin <220> <221> CDS <222> (101)..(649) <223> RXN00198 <400> 83 atgcaggatt agagcagcag caacggagcc agaatcagtc tcccaatttg atatccagcc ccgcgctcct ttccagcgcg ccgattccac tccatggccg atg tac ccc aac ctc 115 Met Tyr Pro Asn Leu 1 ttc cgc acc gca acg gct cac gaa gaa ggc gaa tac atc atc act ggc 163 Phe Arg Thr Ala Thr Ala His Giu Giu Gly Giu Tyr Ilie Ile Thr Gly 15 WO 01/00843 ga t Asp gca Ala t tc Phe cga Arg ttc Phe caa Gin cgc Arg tac Tyr atc Ilie 150 ggt tca Ser tcc Ser ggc Giy gtc Val gca Ala gga Gly ctc Leu 120 gca Ala gaa Giu act gcc Ala acg Thr aac Asn aac Asn gac Asp ttg Leu 105 cgc Arg ggC G ly ggc Gly gca gaa Glu ggt Gly ggc Gly aaa Lys 75 gtt Val1 c ac His tc Ser aac Asn gca Al a 155, cc t atc Ile gaa Giu cac His cg t Arg ctt Leu gaa Giu gaa Giu Gly 140 tgc Cys gta gca Al a cgt Arg 45 gtc Val1 gaa Glu gtt Val1 cta Leu tac Tyr 125 cg t Arg gcc Ala gca ctg Leu ttt Phe gga Gly atc Ile ctt Leu gta Val1 agc Ser cag Gin gc t Ala gca Ala 175 C tC Leu gtc Val acc Thr ggC G ly t tc Phe t tc Phe acc Thr c tg Leu 145 gac Asp cag Gin gcc Aila aac Asn ggc Giy acc Thr acc Thr gac Asp aac Asn 130 atc Ile gcc Ala gac Asp gaa Glu ac c Thr aac Asn gaa Giu ggc Gly gac Asp 115 tcc Ser gtg Val1 gat Asp gtg Val1 PCTIIBOO/00923 cgt gcc 211 Arg Ala gtg gaa 259 Vai Glu caa atc 307 Gin Ile ttc ccc 355 Phe Pro gca gaa 403 Ala Giu 100 cgg ggC 451 Arg Gly cgc gtt 499 Arg Val tgg gca 547 Trp Ala ctc atg 595 Leu Met 165 ccg ctg 643 Pro Leu 180 Giy Glu Thr Aia Leu Pro Vai Ala Vai 170 gct gtc Ala Val tagcttgggt agaaaatgct aga <210> 84 <211> 183 <212> PRT <213> Corynebacterium giutamicum <400> 84 Met Tyr Pro Asn Leu Phe Arg Thr Ala Thr Ala His Glu Giu Gly Glu 1 5 10 Tyr Ile Ilie Thr Giy Asp Giu Ser Ala Asp Glu Ile Ala Ala Leu Gly 25 Leu Ala Glu Arg Ala Ala Gly Ser Thr Leu Gly Glu Arg Lys Phe Ala 40 Val Asn Thr Val Giu Phe His Gly Asn Asn Giy His Val Thr Gly Leu 131 WO 01/00843 PCT/IBOO/00923 55 Thr Gly Asn Gin Ile Arg Val Val Asn Gly Lys Arg Giu Pro Ile Glu 70 75 Gly Thr Giu Phe Pro Phe Giu Ala Asp Leu Val Leu Val Ala Leu Gly 90 Phe Thr Gly Ala Giu Gin Gly Gly Leu Ala His Giu Leu Gly Val Gly 100 105 110 Phe Asp Asp Arg Gly Arg Ile Leu Arg Asp Ser Giu Tyr Arg Ser Pro 115 120 125 Thr Asn Ser Arg Val Tyr Ile Ala Gly Asp Asn Gly Arg Gly Gin Ser 130 135 140 Leu Ile Val Trp, Ala Ilie Ala Giu Gly Arg Ala Cys Ala Ala Ala Ile 145 150 155 160 Asp Ala Asp Leu Met -Gly Glu Thr Ala Leu Pro Val Ala Val Ala Pro 165 170 175 Gin Asp Val Pro Leu Ala Val 180 <210> <211> 305 <212> DNA <213> Corynebacterium glutamicum <22 0> <221> CDS <222> <223> FRXA00198 <400> ctc gtt ctt gtt gca ctt ggc ttc acc ggc gca gaa caa ggc gga ttg 48 Leu Val Leu Val Ala Leu Gly Phe Thr Gly Ala Glu Gin Gly Gly Leu 1 5 10 gca cac gaa cta ggc gta ggt ttc gac gac cgg ggc cgc atc ctc cgc 96 Ala His Giu Leu Gly Val Gly Phe Asp Asp Arg Gly Arg Ile Leu Arg 25 gat tcc gaa tac cgc agc ccc acc aac tcc cgc gtt tac atc gca ggc 144 ASP Ser Giu Tyr Arg Ser Pro Thr Asn Ser Arg Val Tyr Ilie Ala Gly 40 gac aac ggc cgt ggc cag tcc ctg atc gtg tgg gca atc gcc gaa ggc 192 Asp Asn Gly Arg Gly Gin Ser Leu Ile Val Trp Ala Ile Ala Giu Gly 55 cgc gca tgc gcc gca gct atc gac gcc gat ctc atg ggt gaa act gca 240 Arg Ala Cys Ala Ala Ala Ile Asp Ala Asp Leu Met Gly Giu Thr Ala 70 75 ctc cct gta gca gtt gca cca cag gac gtg ccg ctg gct gtc 282 Leu Pro Val Ala Val Ala Pro Gin Asp Val Pro Leu Ala Val WO 01/00843 WO 0100843PCTIIBOOIOO923 tagcttgggt agaaaatgct aga <210> 86 <211> 94 <212> PRT <213> Corynebacteriun glutarnicum <400> 86 Leu Val Leu Val Ala Leu Gly Phe 9] 1 5 Ala His Glu Leu Gly Val Gly Phe 1 Asp Ser Glu Tyr Arg Ser Pro Thr 1 Asp Asn Gly Arg Gly Gin Ser Leu 1 Arg Ala Cys Ala Ala Ala Ilie Asp .1 Leu Pro Val Ala Val Ala Pro Gin 1 Gly Leu Ala Glu Thr <210> 87 <211> 727 <212> DNA <213> Corynebacteriun glutamicun <22 0> <221> CDS <222> (101)..(727) <223> RXN00365 <400> 87 gaagcctacg atcgcttgca cgcgaccaac aatttccccg cccgcaccct gcgaaggcgc ctgcgtgctc ggtatcaacg tca Ser t gg Trp gtc Val cgc Arg gag Glu gta Val1 Gly agc Ser gaa Glu cca Pro gct Ala acc Thr agttcaccgg ccgtttgtgc atg att ctg tca cca met Ile Leu Ser Pro 1 gca ttc cgc gaa ggc Ala Phe Arg Glu Gly ggg ctg tca gtc gcc Gly Leu Ser Val Ala gcg cag cag ctc acc Ala Gln Gin Leu Thr gac gac cgc ctc ggc Asp Asp Arg Leu Gly 115 163 211 259 307 WO 01/00843 WO 0100843PCT/IBOO/00923 ggC Gly atc Ile gta Val ctc Leu cac His aac Asn 150 ggc Gly t tc Phe atc Ile ctc Leu gac Asp ggc Gly ctc Leu ga t Asp 135 cgc Arg aag Lys ggc Gly cgc Arg atg Met cgc Arg acc Thr gca Al a 120 C tC Leu atc Ile aa a Lys acc Thr ccc Pro 200 cgc tac ggc gtg Arg Tyr Gly Val 75 cca gaa tac aaa atg Pro Giu Tyr Lys Met gag Glu 80 gag Giu cgc Arg acc Thr atc Ile G ly 155 atc Ile cgC Arg c aa Gin gcc Ala cca Pro cat His 140 gac Asp atc Ile caa Gin atg Met gc t Ala gtg Va 1 125 gcg Ala gg t Gly gg t Gly gga Gly gaa Giu gaa Giu 110 gcc Ala gca Ala gaa Glu ggc Gly gca Ala 190 ggc Gly ctt Leu ctc Leu tac Tyr 145 cca Pro acc Thr gtc Val1 aca Thr ttc Phe t ca Ser 130 ctc Leu atc Ile Gly ac c Thr aac cgc Asn Arg act ttc Thr Phe 100 gac gcg Asp Ala 115 gtt cca Val Pro acc gcc Thr Ala aac gcc Asn Ala acc gac Thr Asp 180 caa ttt Gin Phe 195 tgg Trp cag Gin atc Ile Gly caa Gin aaa Lys 165 tgc Cys ga t Asp 355 403 451 499 547 595 643 691 cgc gct cct ttc cag cgc Arg Ala Pro Phe Gin Arg 205 gcc gat tcc Ala Asp Ser <210> 88 <211> 209 <212> PRT <213> Corynebacteriui giutamicum <400> 88 Met Ile Leu Ser Pro Ser Lys Thr Phe Giu Leu Giu 1 5 Ala Phe Arg Giu Gly Trp Val Gin Pro Val Ile Pro 25 Gly Leu Ser Val Ala Val Val Gly Ser Gly Pro Ala 40 Ala Gin Gin Leu Thr Arg Ala Gly His Ser Val Thr 55 Asp Asp Arg Leu Gly Gly Leu Met Arg Tyr Gly Val 70 75 Ile Ser Gly Val1 Pro Val Met Leu Phe Glu met Giu Asn Arg Trp Ile Asp Arg Arg Giu Gin met Glu Ala Giu WO 01/00843 WO 0100843PCTIBOO/00923 Gly Leu Leu Tyr 145 Pro Thr Val Ser Thr

ASP

115 Val Thr Asn Thr Gin 195 Phe 100 Ala Pro Ala Ala Asp 180 Phe Ser 105 Thr Asn Asn Val1 Ala 185 Arg Ala Glu Asp Ser 160 Asp Ser Asp <210> 89 <211> 727 <212> DNA <213> Corynebacterium glutamicum <22 0> <221> CDS <222> (101)..(727) <223> FRXA00365 <400> 89 gaagcctacg atcgcttgca cgcgaccaac aatttccccg cccgcaccct gcgaaggcgc ctgcgtgctc ggtatcaacg tca Ser tgg Trp gtc Val1 cgc Arg ggc Gly acg Thr caa Gin ggt Gly ggc Gly atg Met ttt Phe cca Pro tcc Ser cac His cgc Arg gtc gaa Val Giu atg tct Met Ser 30 ctt gcc Leu Ala ttt gaa Phe Giu gaa tac Glu Tyr 135 aaa Lys acc Thr gcc Ala cgc Arg aaa Lys agttcaccgg ccgtttgtgc atg att ctg tca cca Met Ile Leu Ser Pro 1 gca ttc cgc gaa ggc Ala Phe Arg Giu Gly ggg ctg tca gtc gcc Gly Leu Ser Val Ala gcg cag cag ctc acc Ala Gin Gin Leu Thr gac gac cgc ctc ggc Asp Asp Arg Leu Giy atg gag aac cgc tgg Met Giu Asn Arg Trp WO 01/00843 WO 0100843PCT/IBOO/00923 75 atc gac cgc cgc atc gag caa atg Ilie Asp Arg Arg Ile Giu Gin Met gta ggc acc tcg ccg cgc gcc gct Val Giy Thr Ser Pro Arg Ala Ala 105 ctc ctc gca acc ggc acc cca gtg Leu Leu Ala Thr Giy Thr Pro Val 120 125 cac gat ctc aac ggc atc cat gcg His Asp Leu Asn Giy Ile His Ala 135 140 aac cgc atc aac gaa ggc gac ggt Asn Arg Ilie Asn Giu Giy Asp Gly 150 155 ggc aag aaa gtt gtc atc atc ggt Gly Lys Lys Val Val Ile Ile Gly 170 ttc ggc acc gca ctg cgc caa gga Phe Gly Thr Ala Leu Arg Gin Gly 185 atc cgc ccc cgc gct cct ttc.cag Ile Arg Pro Arg Ala Pro Phe Gin 200 205 <210> <211> 209 <2i2> PRT <213> Corynebacterium giutamicun <400> Met Ile Leu Ser Pro Ser Lys Thr 1 5 Aia Phe Arg Giu Gly Trp Vai Gin Gly Leu Ser Val Ala Val Val Giy 40 Ala Gin Gin Leu Thr Arg Ala Gly 55 Asp Asp Arg Leu Gly Gly Leu Met 70 Met Giu Asn Arg.Trp Ile Asp Arg Gly Thr Thr*Phe Gin Val Giy Thr 100 gaa Glu aa Glu 110 gcc gca Pd a gaa Glu Gly gc a Al a 190 cgc Arg Phe Pro 25 Ser His Arg PArg Ser 105 136 gca Al a 95 cta Leu cgc Arg atg Met gtc Vai ggc Giy 175 gaa Giu gcc Ala Giu Val Giy Ser Tyr Ile Pro gag Giu gcg Ala gaa Giu gat

ASP

tc t Ser 160 gac Asp tca Ser ga t Asp Leu Ile Pro Val Giy Giu Arg ggc Gly ctt Leu C tc Leu tac Tyr 145 cca Pro acc Thr gtc Val1 tcC Ser Giu Pro Ala Thr Val1 Gin Ala aca Thr ttc Phe tca S er 130 ctc Leu atc Ile Gly acc Thr Ile Ser Gly Val Pro Met Ala act Thr gac Asp 115 gtt Val ac c Thr aac Asn acc Thr caa Gin 195 Val1 Met Leu Phe Glu Glu Glu 110 WO 01/00843 WO 0100843PCTIBOO/00923 Leu Leu Tyr 145 Pro Thr Val Ser Ile Gly Gin L~ys 165 Cys Asp Leu His Asn 150 Gly Phe Ile Leu Asp 135 Arg Lys Gly Arg Ala 120 Leu Ile Lys Thr Pro 200 Ala Ala Glu Giy Ala 190 Arg Arg Met Val Gly 175 Glu Ala <210> 91 <211> 480 <212> DNA <213> Corynebacteriun glutamicun <22 0> <221> CDS <222> (101)..(457) <223> RXA00366 <400> 91 aaatcatgcc gcgcgatttc caaaaagtac tcaacatcat gccaagaccc agcaatcaag atcatggagg cagtgagcta gga Gly c tg Leu cag Gin tgc Cys ctg Leu acc cgc Arg gag Glu cgc Arg ggC Gly aag Lys acc gag Glu gtc Val tgc Cys aac Asn gaa Giu ggc 137 cgaaacggcc cacgctgagg atg gcc gac cca caa Met Ala Asp Pro Gin 1 cac cgc ccg gtc ccg His Arg Pro Val Pro aag gca ccg gca ggt Lys Aia Pro Ala Giy tgc ggt gtc ccg ttc Cys Gly Vai Pro Phe cct gag tgg aat gat Pro Giu Trp Asn Asp gat cgc ttg cac gcg Asp Arg Leu His Ala tgc ccc gca ccc tgc 115 163 211 259 307 355 403 WO 01/00843 WO 0100843PCTA~BOOIOO923 Thr Asn Asn Phe Pro Giu Phe Thr Gly Arg Leu Cys Pro Ala Pro Cys 95 100 gaa ggc gcc tgc gtg ctc ggt atc aac gat gat tct gtc acc atc aaa 451 Giu Gly Ala Cys Val Leu Gly Ilie Asn Asp Asp Ser Val Thr Ile Lys 105 110 115 aac gtt tgagctggaa atcgtcgaaa aag. 480 ASn Val <210> 92 <211> 119 <212> PRT <213> Corynebacteriun giutamicun <400> 92 Met Ala Asp Pro Gin Gly Phe Ile Lys Tyr Ser Arg Arg Giu Pro Ala 1 5 10 His Arg Pro Val Pro Leu Arg Leu Met Asp His Ser Glu Val Tyr Giu 25 Lys Ala Pro Ala Gly Gin Ile Giu Giu Gin Ala Ala Arg Cys Met Asp 40 Cys Gly Val Pro Phe Cys His Giu Gly Cys Pro Leu Gly Asn Ile Ile 55 Pro Giu Trp, Asn Asp Leu Val Arg Gin Gly Arg Trp Lys Giu Ala Tyr 70 75 Asp Arg Leu His Ala Thr Asn Asn Phe Pro Giu Phe Thr Gly Arg Leu 90 Cys Pro Ala Pro Cys Glu Gly Ala Cys Val Leu Gly Ile Asn Asp Asp 100 105 110 Ser Val Thr Ile Lys Asn Val 115 <210> 93 <211> 1464 <212> DNA <213> Corynebacteriun giutanicun <22 0> <221> CDS <222> (101)..(1441) <223> RXA02072 <400> 93 tacatggtgc cctcaatggg aaccaccaac atcactaaat ggcccaggta cacactttaa aatcgtgcgc gcatgcagcc gagatgggaa cgaggaaatc atg aca gtt gat gag 115 Met Thr Val Asp Giu 1 cag gtc tct aac tat tac gac atg ctt ctg aag cgc aat gct ggc gag 163 138 WO 01/00843 Gin Val Ser Asn Tyr Tyr Asp Met Leu Leu Lys Arg Asn Ala 15 cac His aag Lys cct Pro cag Gin gga Gly att Ile 105 ggc Gly ggc Gly gag Giu atc Ile cgc Arg 185 acc Thr gtt Val1 ggc Gly att Ile cag Gin gac Asp gag Giu gtc Val1 c ca Pro gtg Val1 ctg Leu aag Lys ctg Leu gga Gly 170 a tg Met tgg Trp tac Tyr c ag Gin gaa Glu gtg Val1 cat His cag Gin gtC Val1 aag Lys t tc Phe atc Ile gat Asp 140 cgc Arg ggt Giy aac Asn gga G iy gt~g Vai 220 atc Ile gc t Ala gca Al a tac Tyr ctc Leu aac Asn ggc Giy ctg Leu ggt Gly 125 c tg Leu cac His ggc Gly cag Gin tcc Ser 205 agt Ser atc Ile cag Gin gaa Glu ggt Gly gtg Val1 cgc Arg t tc Phe cag Gin ggt G iy cg t Arg 145 tac Tyr gg t Gly ggc Giy acc Thr aag Lys 225 tcc Ser tct ttg Ser Leu ctc* atc Leu Ile cct tgg Pro Trp gtg cag Val Gin cac cca His Pro atc ttt Ile Phe 115 gga tcc Gly Ser 130 ttc tgc Phe Cys cgc gac Arg Asp tac ctg Tyr Leu gtt ttg Vai Leu 195 gag gca Giu Ala 210 gct aag Aia Lys ggc aac Gly Asn PCTIIBOO/00923 Gly Giu aag atc 21i Lys Ile cag cgc 259 Gin Arg gtt gat 307 Vai Asp ttc aac 355 Phe Asn tct gta 403 Ser Val 100 aaa aac 451 Lys Asn gac ttc 499 Asp Phe cag tcc 547 Gin Ser gtt cct 595 Val Pro 165 ttt ggc 643 Phe Gly 180 acc ggt 691 Thr Gly act ggc 739 Thr Gly ggc gag 787 Gly Giu gta gca 835 Val Ala 245 gaa ctc ggc Glu Leu Gly gca acc gtt att ggt Ala Thr Val Ile Gly WO 01/00843 WO 0100843PCTIBOO/00923 260 gac gtg Asp Val ttc tcc Phe Ser gct aag Ala Lys gat Asp ggt tgg gtt Gly Trp, Val cat His 270 cct aac ggc Pro Asn Gly tac gcc 1027 Tyr Ala 295 tgg gat 1075 Trp Asp 310 ctc aac 1123 Leu Asn gtt gct 1171 Val Ala ttc cgt 1219 Phe Arg ggt ggc 1267 Gly Gly 375 gat tcc 1315 Asp Ser 390 aag aac 1363 Lys Asn gag aac 1411 Giu Asn gct gac 1461 Ala Asp ctc cgc gaa atc aag Leu Arg Giu Ile Lys 280 gac gaa gtt gaa ggc Asp Glu Val Giu Gly 300 ctc aag tgc gat atc Leu Lys Cys Asp Ile 315 ggc gag aac gct aag Gly Giu Asn Ala Lys 330 gaa ggc gcg aac atg Giu Glv Ala Asn Met gaa gtt Giu Val 285 gca acc Ala Thr cgt cgc gca Arg Arg Ala tac cac acc Tyr His Thr 305 cgc gta tcc gtg 979 Arg Val Ser Val 290 gac ggt tcc atc Asp Gly Ser Ile gct ctt cct tgt Ala Leu Pro Cys 320 gca Ala act cag aac gag Thr Gin Asn Glu 325 act ctt gca Thr Leu Ala 335 cct tcc acc Pro Ser Thr 350 gac aac ggc tgc cgt ttc Asp Asn Gly Cys Arg Phe 340 cct gag gct gtt gag gtc Pro Glu Ala Val Giu Val 345 gag Giu 360 gt t Val tgg Trp cgc gac atc cgc ttc Arg Asp Ile Arg Phe 365 gca acc tcc gct ctg Ala Thr Ser Ala Leu 380 agc ttc gag tac acc Ser Phe Giu Tyr Thr gga cca Gly Pro ggc aag gca Gly Lys Ala 370 355 gct aac gct Ala Asn Ala gag atg cag cag aac gct tcg cgc Giu Met Gin Gin Asn Ala Ser Arg 385 gac gag cgc ctc cag gtg atc atg Asp Giu Arg Leu Gin Val Ile Met 395 atc ttc aag acc Ile Phe Lys Thr 410 400 tgt gca gag acc gca Cys Ala Giu Thr Ala 415 gat tac Asp Tyr 425 gcg atg Ala Met 440 gtt gtc ggc gct aac att Val Vai Gly Ala Asn Ile 430 ctg gca cag ggc gtc atc Leu Ala Gin Giy Val Ile 445 gc t Ala gca gag tat gga cac Ala Giu Tyr Gly His 420 ggc ttc aag aag gta Gly Phe Lys Lys Val 435 gacccct gcgctttact WO 01/00843 WO 0100843PCTITBOOIOO923 taa 1464 <210> 94 <211> 447 <212> PRT <213> Corynebacterium giutamicun <400> 94 Met Thr Val Asp Giu Gin Val Ser Asn Tyr Tyr Asp Met Leu Leu Lys 1 5 10 Arg Asn Ala Gly Giu Pro Giu Phe His Gin Ala Val Ala Giu Val Leu 25 Giu Ser Leu Lys Ile Val Leu Giu Lys Asp Pro His Tyr Ala Asp Tyr 40 Gly Leu Ile Gin Arg Leu Cys Giu Pro Giu Arg Gin Leu Ile Phe Arg 55 Val Pro Trp Val Asp Asp Gin Giy Gin Val His Val Asn Arg Gly Phe 70 75 Arg Val Gin Phe Asn Ser Ala Leu Gly Pro Tyr Lys Giy Gly Leu Arg 90 Phe His Pro Ser Val Asn Leu Gly Ile Val Lys Phe Leu Gly Phe Giu 100 105 110 Gin Ile Phe Lys Asn Ser Leu Thr Gly Leu Pro Ile Gly Gly Gly Lys 115 120 125 Gly Giy Ser Asp Phe Asp Pro Lys Gly Lys Ser Asp Leu Giu Ile Met 130 135 140 Arg Phe Cys Gin Ser Phe Met Thr Giu Leu His Arg His Ile Gly Glu 145 150 155 160 Tyr Arg Asp Vai Pro Ala Giy Asn Ile Giy Val Gly Gly His Giu Ile 165 170 175 Gly Tyr Leu Phe Gly His Tyr Arg Arg Met Ala Asn Gin His Glu Ser 180 185 190 Gly Val Leu Thr Gly Lys Gly Leu Thr Trp Gly Gly Ser Leu Val Arg 195 200 205 Thr Giu Ala Thr Gly Tyr Gly Cys Val Tyr Phe Val Ser Giu Met Ile 210 215 220 Lys Ala Lys Gly Giu Ser Ile Ser Gly Gin Lys Ile Ile Val Ser Gly 225 230 235 240 Ser Gly Asn Val Ala Thr Tyr Ala Ile Giu Lys Ala Gin Giu Leu Giy 245 250 255 Ala Thr Val Ile Gly Phe Ser Asp Ser Ser Gly Trp Val His Thr Pro 260 265 270 WO 01/00843 Asn Gly Val 275 Ala Arg Val 290 Thr Asp Gly 305 Ala Thr Gin Asn Giy Cys Giu Ala Val 355 Lys Ala Ala 370 Gin Asn Ala 385 Leu Gin Val Ala Giu Tyr Gly Phe Lys 435 PCTIBOO/00923 Asp S er Ser Asn Arg 340 Giu Asn Ser Ile G ly 420 Lys Ala Tyr Trp 310 Leu Val1 Phe Giy Asp 390 Lys Giu Ala Leu 280 Asp Leu Gly Giu Giu 360 Val Trp Ile Asp Ala 440 Arg Giu Lys Giu Gly 345 Arg Ala Ser Phe Tyr 425 Met le Giu Asp 315 Aia Asn Ile Ser Giu 395 Thr Vai Ala Giu 285 Ala Ala Thr Pro Phe 365 Leu Thr Al a Ala Gly 445 Arg His Cys 320 Asp Pro Gly Gin Arg 400 Ala Ala <210> <211> 1461 <212> DNA <213> Corynebacterium giutamicum <2 <221> CDS <222> (1438) <223> RXA00323 <400> cgcgacatcc ttgagtaact ctgagaaaaa ctacccccga atgcgcagtc gatgtcccat cgctgcgtag attagttttc gaa ttt gta ctc agc gcc att gaa gaa cgc gac Giu Phe Val Leu Ser Ala Ile Giu Giu Arg Asp 15 cta tgg ttc act gac att ctt ggc cac ttg aag Leu Trp Phe Thr Asp Ile Leu Gly His Leu Lys 30 cct gca gaa cta gag tct gcg ttg gaa gaa ggc Pro Ala Giu Leu Giu Ser Ala Leu Giu Giu Gly 142 tgggagtata aaagtggcaa atg aac agc gaa cag Met Asn Ser Giu Gin 1 att aag ttt gtg cgt Ile Lys Phe Val Arg tca gtg gtt gtg gct Ser Val Val Val Ala atc gga ttc gat ggc Ile Gly Phe Asp Gly WO 01/00843 tca gcc att Ser Ala Ilie cgc cca gat Arg Pro Asp tca aaa ctg Ser Lys Leu gga cag cca Gly Gin Pro caa cta gct Gin Leu Aia 120 gag ttc tat Giu Phe Tyr 135 ccc act gac Pro Thr Asp 150 ccg aat ttc Pro Asn Phe cct gtc gag Pro Val Giu gat tta cgc Asp Leu Arg 200 ttc cgc tac Phe Arg Tyr 215 tca ttt atg Ser Phe Met 230 acg cac atg Thr His Met gac gat tct Asp Asp Ser PCT/IBOO/00923 t ac Tyr aca Thr 75 gca Ala tc t Ser gaa Giu caa Gin gga Gly 155 aac Asn c ac His ga t Asp aa a Lys cca Pro 235 ttt Phe ctg Leu gcg cgt atc Ala Arg Ile ttc cag gtc Phe Gin Val cgc ctg ttt Arg Leu Phe gac ccg cgc Asp Pro Arg 110 ggc ttg acc Gly Leu Thr 125 agc ctt cgc Ser Leu Arg 140 tat ttc gac Tyr Phe Asp gcg atg gta Ala Met Val cat gaa act His Giu Thr 190 gcg ctc acc Ala Leu Thr 205 cag gtg gca Gin Val Ala 220 ttc caa gaa Phe Gin Giu gag ggc gat Giu Gly Asp tcc aaa acc Ser Lys Thr 270 gaa ttc acc Giu Phe Thr 285 acc Thr gcg Ala atg Met agg Arg 115 cca Pro cca Pro aa t Asn c tc Leu caa Gin 195 atc Ile gtc Val gcc Ala cac His a tc Ile 275 att Ile ggc Gly cca Pro 100 cag Gin gag Giu cct Pro gag Giu ggc Gly 180 gaa Giu atg Met ggg Gly atg Met ga t Asp 260 gc t Ala gcc Ala atc Ile gac Asp gtc Val att Ile gtg Val1 gcg Ala 165 atc Ile atc Ile ac c Thr gca Ala cac His 245 cca Pro gga G ly gtc Val1 atc ttg cat cac gct cca Ile Leu His His Ala Pro 280 gct gtg acc aac cag tgg Ala Val Thr Asn Gin Trp 290 WO 01/00843 WO 0100843PCT[IBOO/00923 aat tcc 1027 Asn Ser 295 acc tgg 1075 Thr Trp 310 cgt ttg 1123 Arg Leu acc gct 1171 Thr Ala ttg aaa 1219 Leu Lys gat atc 1267 Asp Ilie 375 gat ctg 1315 Asp Leu 390 gag ctt 1363 Glu Leu cgc aat 1411 Arg Asn tgg gag 1458 Trp Giu tac aaa cgc atc gtg Tyr Lys Arg Ile Val 300 ggt gta tct aat cgt Gly Val Ser Asn Arg 315 aat aag gag gag tcg Asn Lys Glu Glu Ser 330 tgt aac cca tat ttg Cys Asn Pro Tyr Leu tac gga aac gaa gct cca act gcg gca Tyr Gly Asn Glu Ala Pro Thr Ala Ala 305 tct gcg ctg gtt cgt gtt cct acc tac Ser Ala Leu Val Arg Val Pro Thr Tyr 320 cgc cgg Arg Arg gcg ttt Ala Phe 350 gtg Val 335 gag Glu gtg cgt ctt cct gat Val Arg Leu Pro Asp 340 345 ggc att Gly Ile tca gtg atg ctc ggc gct ggt Ser Val Met Leu Gly Ala Gly 355 ctc gac gag cca gct gag gac Leu Asp Giu Pro Ala Glu Asp 370 aaa gaa ggt tat Lys Glu Gly Tyr 365 gag Glu aac ttg agc ttc Asn Leu Ser Phe 380 cgg gaa cgt cgc gcc atg ggc tac aac Arg Giu Arg Arg Ala Met Gly Tyr Asn 385 cag gca ctg cgc caa atg gaa aag tca Gin Ala Leu Arg Gin Met Giu Lys Ser cca agc agc ctt Pro Ser Ser Leu 395 gtt gct gac atc Val Ala Asp Ile 410 aag tgg cgt gaa Lys Trp Arg Giu 425 ctc cga aac aat Leu Arg Asn Asn 440 gat Asp ctc ggt gag cac Leu Gly Glu His 415 tgg cgt gac tac Trp, Arg Asp Tyr 430 ctt gat tac tag~ Leu Asp Tyr 445 gtt ttt gag ttt ttc ttg Val Phe Glu Phe Phe Leu 420 caa gag cag atc act ccg Gin Glu Gin Ile Thr Pro 435 icttttg cactccaatg gaa 1461 <210> 96 <211> 446 <212> PRT <213> Corynebacterium giutamicum <400> 96 .144 WO 01/00843 Met Asn Ser 1 Ile Lys Phe Ser Val Val Ilie Gly Phe Ala Asp Thr Leu Glu Ala Val Thr Met Leu Arg Arg 115 Ile Ser Pro 130 Gly Leu Pro 145 Thr Phe Asn Giu Giu Leu Giy Gin Gin 195 Asp Asn Ilie 210 Gin Gly Val 225 Giy Ser Ala Ala Phe His Gin Phe Ile 275 Thr Asn Gin 290 Ala Pro Thr 305 Giu Vali Val1 Asp Ile Gly Pro 100 Gin Giu Pro Giu Gly 180 Giu Met Gly Met Asp 260 Ala Trp Ala Gin 5 Arg Ala Gly Ala Ile Asp Val1 Ilie Val1 Ala 165 Ile Ile Thr Ala His 245 Pro G ly Val1 Ala Ile Leu Al a Ala Phe Arg Asp Gly Ser 140 Tyr Ala His Ala Gin 220 Phe Giu Ser Glu Val 300 Arg Giu Gly Leu Arg Gin Leu Pro Leu 125 Leu Phe Met Giu Leu 205 Val1 Gin Gly Lys Phe 285 Tyr Ser PCTIiBOO/00923 rg Asp eu Lys iu Gly er Glu eu Pro ys Asp in Val ys Met hr Asn in Ala 160 la Leu la Pro et Ala Arg Val Pro Thr Tyr Arg Leu Asn Lys Glu Glu Ser Arg Arg Val Giu WO 01/00843 Val Met Glu Ala 385 Gin Phe Giu Pro 340 Ala Glu Tyr Lys Phe 420 Thr 325 Asp Gly Asp Asn Ser 405 Leu Pro Thr Leu Asp Asp 390 Glu Arg Trp Phe 350 Giu Glu Ala Giu Asp 430 Tyr PCTJIBOO/00923 335 Ser Val Leu Asp Arg Arg Leu Arg 400 His Val 415 Tyr Gin <210> 97 <211> 1554 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(1531) <223> RXA00335 <400> 97 actacatttg cagccaagtc tactacttga tcttcaaagg tacaaataaa ccgttccgcc catgtcaatg aggagtcacc ccg Pro gac Asp cca Pro gac Asp aa t Asn aag Lys gac Asp gat Asp cg t Arg ctc Leu 75 t tc Phe ctt Leu gca Ala ggc G ly gga Gly tcagcaattg tgaacaaagc gtg gcg ttt gaa acc Val Ala Phe Glu Thr 1 aac gtc gag ttc gtt Asn Val Giu Phe Val cag cac ttc agc atc Gin His Phe Ser Ile gaa ggt ctc gca ttc Giu Gly Leu Ala Phe gac gaa tct gac atg Asp Giu Ser Asp Met gat cca ttc cgc aag Asp Pro Phe Arg Lys 115 163 211 259 307 355 WO 01/00843 gca aag ac Ala Lys Tb gag gca tt Glu Ala Ph tac ctg gc Tyr Leu Al 12 gct gag tt Ala Glu Ph 135 tcc ggc tt Ser Gly Ph 150 aag gaa ac Lys Glu Tb gtc aag gg Val Lys Gl gtg cgc ga Val Arg As 2C gag cgt tt Glu Arg Ph 215 cgc ttc aa Arg Phe As 230 tac atc at Tyr Ile Il atg cct aa Met Pro Ly cag tcc ct Gln Ser Le 28 tac gca gg 1027 Tyr Ala Gl 295 cac cac gc 1075 His His Al 310 PCTIiBOO/00923 aag Lys cca Pro att Ile gac Asp 140 gat Asp ggc Gly cc a Pro cgc Arg gtc Val1 220 cac His gc t Al a ggc Gly ggc Gly atc Ile 300 ctg Leu ttc Phe cgc Arg gca Ala 125 tcc Ser ac c Thr acc Thr gta Val1 aac Asn 205 ggt Gly gcg Ala cgc Arg gac Asp aag Lys 285 gcc Ala gcg Ala ttc Phe aa c Asn 110 gac Asp gtt Val1 gaa Glu cc a Pro gca Al a 190 ctc Leu ggc Gly gca Ala C tC Leu aac Asn 270 cca Pro cgc Arg ttc Phe gt t Val1 gtg Val acc Thr cgc Arg gaa Glu aac Asn 175 cca Pro gca Ala gga G ly gat Asp C ac His 255 gg t Gly ctc Leu tac Tyr acc Thr acc Thr 100 gag Glu gcc Ala atg Met cgt Arg aac Asn 180 gtt Val1 gct Ala aac Asn ttc Phe acc Thr 260 gc t Ala tcc Ser atc Ile aac Asn cgc Arg cag Gln gag Glu aac Asn ggc Gly.

165 cgc Arg gac Asp Ctt Leu tac Tyr aag Lys 245 ttc Phe cac His ggc Gly ctg Leu tcc Ser 325 403 WO 01/00843 WO 0100843PCTIBOO/00923 tac cac 1123 Tyr His tca cag 1171 Ser Gin aac ccg 1219 Asn Pro aac cca 1267 Asn Pro 375 atc aag 1315 Ile Lys 390 gaa cta 1363 Giu Leu ctg gaa 1411 Leu Glu gag tct 1459 Giu Ser aag tac 1507 Lys Tyr 455 gaa ttc 1554 Giu Phe 470 cgt ctg gtt Arg Leu Val 330 cgc aac cgt Arg Asn Arg cca ggc ttc gag Pro Giy Phe Giu tcc gct gct gtc Ser Ala Ala Vai 350 cgc atc gaa ttc Arg Ile Glu Phe 345 aag gca LYS Ala 360 aag Lys 365 tac ctg ggc ttt gca Tyr Leu Gly Phe Ala 380 gcg atg Ala Met cac gct His Ala gct cca atc aac ctg gtg tac Ala Pro Ile Asn Leu Val Tyr 335 340 cgt atc cca atc acc gga tcc Arg Ile Pro Ile Thr Gly Ser 355 cgc gct cca gac cca tca ggc Arg Ala Pro Asp Pro Ser Gly 370 atg atg gcc ggc ctc gac ggc Met Met Ala Gly Leu Asp Gly 385 cca gtg gac aag gac ctc tac Pro Val Asp Lys Asp Leu Tyr 400 405 att cca cag gca cca acc tcc Ile Pro Gin Ala Pro Thr Ser 415 420 gaa gac acc gac ttc ctc acc Giu Asp Thr Asp Phe Leu Thr aac cgc atc gag Asn Arg Ile Glu 395 cca cca gag gaa Pro Pro Glu Giu 410 gca tcc ctg aag Ala Ser Leu Ly's cca Pro gct gca tcc Ala Ala Ser gca ctg cag Ala Leu Gin 430 425 gac Asp 440 gtc ttc acc gag gat ctc Val Phe Thr Giu Asp Leu 445 435 atc gag gcg tac atc cag tac Ile Giu Ala Tyr Ile Gin Tyr 450 cgc ctg cgc cca acc ccg cag Arg Leu Arg Pro Thr Pro Gin 465 gac aac gag atc tcc cca Asp Asn Glu Ile Ser Pro 460 gaa ttg tac ttc gac tgc Giu Leu Tyr Phe Asp Cys 475 gtt Val1 taattcactt agctagccga tag <210> 98 <211> 477 <212> PRT <213> Corynebacteriui giutanicun <400> 98 Val Ala Phe Giu Thr Pro Giu Giu Ile Val Lys Phe Ilie Lys Asp Giu 1 5 10 WO 01/00843 WO 0100843PCTIIBOO/00923 Asn Val Glu Phe Val Asp Val Arg Phe Thr Asp Leu Pro Gly Thr Giu 25 Gin Giu Asp Asp Asp Arg Asn Ser 145 Trp, Gly Asp Ser Gin 225 Ile Lys Gly His Ile 305 Ala Phe Leu Ser Phe Phe Ala 115 Gly Glu Asn Lys Thr 195 Phe Ile Thr Ala His 275 Glu Gly Leu Ile Phe Met Lys Arg Gin Glu Asn Gly 165 Arg Asp Leu Tyr Lys 245 Phe His Gly Leu Ser 325 Pro Asp Asn Ala Giu Tyr Aia Ser 150 Lys Vali Vali Giu Arg 230 Tyr Met Gin Tyr His 310 Tyr Ala 40 Ser Leu Thr Phe Ala 120 Phe Phe Thr Gly Asp 200 Phe Asn Ile Lys Leu 280 Gly Ala Arg Asp Phe Thr Phe Arg Ala 125 Ser Thr Thr Val Asn 205 Gly Ala Arg Asp Lys 285 Ala Ala Phe Thr Thr Ala Phe Asn 110 Asp Val1 Giu Pro Aia 190 Leu Gly Ala Leu Asn 270 Pro Arg Phe Giu Ile Asn Leu Val Tyr Ser Gin Arg Asn Arg Ser Ala Ala Val Arg Ile WO 01/00843 WO 0100843PCTIBOO/00923 340 345 Pro Ile Thr Gly Ser Asn Pro Lys Ala Lys Arg 355 360 Pro Asp Pro Ser Gly Asn Pro Tyr Leu Gly Phe 370 375 Ala Gly Leu Asp Gly Ile Lys Asn Arg Ile Giu 385 390 395 Asp Lys Asp Leu Tyr Giu Leu Pro Pro Giu Giu 405 410 Gin Ala Pro Thr Ser Leu Glu Ala Ser Leu Lys 420 425 Thr Asp Phe Leu Thr Giu Ser Asp Val Phe Thr 435 440 Ala Tyr Ilie Gin Tyr Lys Tyr Asp Asn Giu Ile 450 455 Arg Pro Thr Pro Gin Giu Phe Giu Leu Tyr Phe 465 470 475 <210> 99 <211> 3258 <212> DNA <213> Corynebacterium giutainicum <220> <221> CDS <222> (101)..(3235) <223> RXA00324 <400> 99 tccgtgggag ctccgaaaca atcttgatta ctagactttt cggcgaccca attgcgaccc gatgaaggag gggagaagct Ile Ala 380 Pro Ala Ala Giu Se r 460 Asp 350 Phe Met Ala Ser Gin 430 Leu Val aga Arg gtt Val1 cat His tca Ser tat cgt Arg t ta Leu tgg Trp ggC Gly ctt aaa Lys tcg Ser cgc Arg ga t Asp gaa ggc Gly tct Ser gag Giu gtc Val ggC ttt Phe gag Giu 30 tc t S er gcg Ala gag 150 gcactccaat ggaaacccta atg tca gga ccg tta Met Ser Gly Pro Leu 1 gac cca ctg cca aaa Asp Pro Leu Pro Lys caa gca gat cta gag Gin Ala Asp Leu Giu ttg ttg tgg ggc ttg Leu Leu Trp Gly Leu ctt ctt att cgg ctg Leu Leu Ile Arg Leu cga aac gag ctt gat WO 01/00843 Tyr Gin Ala caa gag att Gin Giu Ilie ttg ggt ggt Leu Giy Giy cag tgg aaa Gin Trp Lys 120 cag gcg ctg Gin Ala Leu 135 gag gat ttc Giu Asp Phe 150 cct ggt ttt Pro Gly Phe ttg aaa tgg Leu Lys Trp tta gcg ggt Leu Ala Gly 200 gtt ccg ttt Val Pro Phe 215 gct ttg act Ala Leu Thr 230 aag ccg gtt Lys Pro Vai gcg cag gaa Aia Gin Glu gag ccg gca Giu Pro Ala 280 atc ggt agc 1027 Leu cgc Arg tcc Ser 105 C tC Leu c tg Leu agc Ser tac Tyr act Thr 185 ac c Thr agc Ser gc t Al a ga t Asp t tg Leu 265 aac Asn aac Gly aaa Lys gg t Gly ga t Asp 125 aaa Lys aac Asn gtt Vali t tg Leu gac Asp 205 atg Met gtg Val tct Ser tca Ser aca Thr 285 gag Giu cta Leu ga t Asp 110 gcg Ala gc t Ala att Ile acc Thr c tg Leu 1 90 atg Met cag Gin gca Ala gtc Val1 gat Asp 270 cgc Arg gtg Asp cga Arg cac His cca Pro cag Gin gcc Ala ggg Gly 175 acc Thr cgg Arg C tC Leu att Ile atc Ile 255 gtg Val1 ac c Thr ga t Ala 80 gtc Val1 ttg Leu tcg Ser cct Pro cga Arg 160 cc t Pro cgg Arg aga Arg agc Ser gcc Ala 240 gcg Ala gac

ASP

gca Ala gca Glu ttt Phe aat Asn 115 gag Glu ctt Leu t tg Leu gag Glu gcg Ala 195 9gc Gly gc t Ala tat Tyr aaa Lys ttt Phe 275 c tc Leu cgc PCTIBOO/00923 Leu Asp gca ttg 403 Ala Leu 100 cct ttg 451 Pro Leu atg ttt 499 Met Phe gag gtt 547 Giu Val agc acg 595 Ser Thr 165 cga gtc 643 Arg Val 180 cat gat 691 His Asp gat cct 739 Asp Pro gat gct 787 Asp Ala ggt gaa 835 Gly Giu 245 tgt ggc 883 Cys Gly 260 gtt gca 931 Val Aia att cgc 979 Ile Arg cca gaa 1ie Gly 295 Ser Asn Ser Phe Phe Glu Val Asp Ala Ala Leu Arg Pro Glu WO 01/00843 WO 0100843PCT/iBOOIOO923 ggt aaa 1075 Gly Lys 310 tac aag 1123 Tyr Lys cgt ccc 1171 Arg Pro ctt tca 1219 Leu Ser gat gtc 1267 Asp Val 375 ttg cgt 1315 Leu Arg 390 gag ttt 1363 Giu Phe acg ttg 1411 Thr Leu cag gga 1459 Gin Gly gag ttt 1507 Giu Phe 455 cgc act 1555 Arg Thr 470 ttg gcg 1603 agt ggc gct Ser Gly Ala cgc tgg gcg Arg Trp Ala 330 ctt Leu 315 gaa Giu gtg cgc tct ttg gat Val Arg Ser Leu ASP 320 acc tgg gag ttt cag Thr Trp Giu Phe Gin 335 att aac ctt ggg cag Ile Asn Leu Gly Gin 350 acg gct agc cag cgg Thr Ala Ser Gin Arcr tcc cat atg gcg tat Ser His Met Ala Tyr 325 gca ctg ctg aaa gct Ala Leu Leu Lys Ala 340 tcc tat gtg gat gct Ser Tyr Val Asp Ala 355 gaa tca ttt gtc aca Giu Ser Phe Val Thr atg acg ggt gat Met Thr Gly Asp 345 ccg ttg att tgg Pro Leu Ile Trp 360 caa gct atg cgc Gin Ala Met Arg gat cgt gag ctg Asp Arg Giu Leu 395 gct gtc cag ctc Ala Val Gin Leu 410 cgg gtt cgg tca Arg Val Arg Ser 365 cgt cga Arg Arg 380 gtg Val1 370 ttg gac aat gtt ccg gaa gac Leu Asp Asn Val Pro Giu Asp 385 aag ctt ggt cgc ggt Lys Leu Gly Arg Gly 400 ctt cag atg gtg cat Leu Gin Met Val His 415 ggt ttg agg gat gtg Gly Leu Arg Asp Val 405 ggt cgc att gat gag Gly Arg Ile Asp Giu 420 acg gta aat Thr Val Asn 425 tat gtg ggt cgt Tyr Val Gly Arg 440 ttg cgt ctg ttg Leu Arg Leu Leu cac ttg tta ccg His Leu Leu Pro 475 cgc gct tct ggg 430 gaa gac ggg Giu Asp Gly 445 gag cat cgc Giu His Arg gct ttg cat gtg ttg gtt gat Ala Leu His Val Leu Val Asp 435 cat aat ctc att gag tcg tat His Asn Leu Ile Giu Ser Tyr 450 ctt caa ttg gag cgg atc aag Leu Gin Leu Giu Arg Ile Lys 465 aaa cct gat gac cga Lys Pro Asp Asp Arg 480 ttt act ggt tcg atg atg aat atg cgc tgg Met Asn Met Arg Trp 485 gag caa agt tcg gcc Leu Ala Arg Ala Ser Gly Phe Thr Gly Ser Met Giu Gin Ser Ser Ala 500 WO 01/00843 WO 0100843PCTIiBOO/00923 aaa gct 1651 Lys Ala ttg cat 1699 Leu His ttg agc 1747 Leu Ser 535 ttg gog 1795 Leu Ala 550 act gct 1843 Thr Ala ttg ctg 1891 Leu Leu gcg gga 1939 Ala Gly agc tgg 1987 Ser Trp 615 ttg atg 2035 Leu Met 630 tcc act 2083 Ser Thr aaa ttg 2131 Lys Leu acg gtg 2179 atg gaa cgg Met Glu Arg 505 agt cag ctg Ser Gin Leu cat ttg cgt aag His Leu Arg Lys 510 ttt tat cgg cca Phe Tyr Arg Pro gtt cgt ttg cag att cag tcg Val Arg Leu Gin Ile Gin Ser 515 ctg ctg aac tct gtg gtc aac Leu Leu Asn Ser Val Val Asn 530 ccg gat gct gca aag cta caa Pro Asp Ala Ala Lys Leu Gin 525 gat gcc atc agg ASP Ala Ile Arg 540 ttg Leu tc t Ser gca ttg gga tac Ala Leu Giy Tyr 555 ctt gca tca gga Leu Ala Ser Gly 570 ccc acg ttg atg Pro Thr Leu Met ctg cat cca tca Leu His Pro Ser gct agc cgt aaa Ala Ser Arg Lys cgt Arg 560 tat gaa cac ctg Tyr Glu His Leu 565 gag tgg ctg Giu Trp Leu 590 gcc aag att cag gcg atg Ala Lys Ile Gln Ala Met 580 caa aca gct gaa cca gat Gin Thr Ala Glu Pro Asp 595 gat gct tcc tat gat cgc Asp Ala Ser Tyr Asp Arg 585 ttg ctg Leu Leu aat tac cgc Asn Tyr Arg cgc atg ctg Arg Met Leu aag Lys 605 c tt Leu tct Ser ttg Leu 620 cgt gat gag ggc Arg Asp Glu Giy tct ccc tat att Ser Pro Tyr Ile gta Val1 625 610 gtg ggg cag cgg Val Giy Gin Arg cgt att ttg gga Arg Ilie Leu Giy 635 ccg gac ttt atg Pro Asp Phe Met 650 ctt gct act gca Leu Ala Thr Ala aat Asn tct gaa ctg att atc Ser Giu Leu Ile Ile 645 gcg gcg tct ggt cct Ala Ala Ser Gly Pro 660 aaa cag ctg Lys Gin Leu ccg act cag Pro Thr Gln ggt Gly 655 665 tcg cgt gtt gtg aaa gca atc aag gca Val Val Lys Ala Ile Lys Ala 675 cgg gcg atc cag gct gca cga cat gag tca cct Thr Vai Ser Arg His Giu Ser Pro Asp Arg Ala Ile Gln Ala Ala Arg WO 01/00843 WO 0100843PCTIBOOIOO923 tcg ctg 2227 Ser Leu 695 aac atg 2275 Asn Met 710 gat gcg 2323 Asp Ala aac gat 2371 Asn Asp ggc atg 2419 Giy Met gat gtg 2467 Asp Val 775 gcc gtc 2515 Ala Val 790 gcg cag 2563 Ala Gin cct gaa 2611 Pro Giu aag tac 2659 Lys Tyr agg gct 2707 Arg Ala 855 gag tcg 2755 Glu Ser 870 agg agg cag gag ctg Arg Arg Gin Giu Leu 700 gca cgc att gcc tct gct gat ttg ctc Ala Arg Ile Ala Ser Ala Asp Leu Leu 705 gta tgc caa agc ttg tca cta gtc tgg Val Cys Gin Ser Leu Ser Leu Val Trp ctc act gtt cag Leu Thr Val Gin 715 gtg ttg gat gct Val Leu Asp Ala 730 gaa Glu 720 gcc ttg gat gcg gaa Ala Leu Asp Ala Glu atc cgt gct gca Ile Arg Ala Ala 740 cca cag Pro Gin 745 ggc cgt Gly Arg 760 atg ttt met Phe aaa cca gat cag cct Lys Pro Asp Gin Pro 750 ttg ggt gga gca gaa Leu Gly Gly Ala Glu 765 gta tgc gag ccg gta Val Cys Glu Pro Val 780 gcc aat att tct gtg atc Ala Asn Ile Ser Val Ile 755 gga tac ggt tct gat gcc Gly Tyr Gly Ser Asp Ala 770 ctt Leu gcc ggt Ala Gly aca tgg tct att Thr Trp Ser Ile 795 cct tcc ggt gat Pro Ser Gly Asp 810 gct atc tgt gat tcc Ala Ile Cys Asp Ser 800 cca cct ttg gag gtg Pro Pro Leu Giu Val 815 gtg gaa gag cat gag Val Giu Giu His Giu 785 atg cgg tcg agg ctt Met Arg Ser Arg Leu 805 gat ctg ggg ctg cgt Asp Leu Gly Leu Arg 820 ggg aga Gly Arg 825 tac gaa Tyr Glu tct ggt Ser Gly gcg att gtg Ala Ile Val 830 aag tgg ggt gaa act Lys Trp Gly Giu Thr 845 gtt gct ggt gat cgt Val Ala Gly Asp Arg cgc acc gtt gat tcc tat gtg Arg Thr Val Asp Ser Tyr Val 835 tgg gag att cag gcg ctg ctg Trp Glu Ile Gin Ala Leu Leu 850 gag ctg ggt att aag ttc ttg Giu Leu Gly Ile Lys Phe Leu tgg Trp att Ile 860 gat cgt ttc cgc Asp Arg Phe Arg 865 tac cca gtt gac ggg Tyr Pro Val Asp Gly 880 gca acg cag gcg Ala Thr Gin Ala 885 875 WO 01/00843 cag ctt cgt 2803 Gin Leu Arg PCTAIBOO/00923 gaa gtt cgt cga att aag gcg agg gtg gat aat gag agg Giu Val 890 ctt ccg 2851 Leu Pro gcg tta 2899 Ala Leu gct cat 2947 Ala His 935 gaa gtg 2995 Giu Vai 950 ctt cgg 3043 Leu Arg ctg gtc 3091 Leu Vai cgc ggg Arg Gly 905 act gac Thr Asp gc t Ala atc Ile Arg Arg Ile Lys gat cga aat acc Asp Arg Asn Thr 910 gag tgg act gtg Giu Ti-p Thi- Val 895 cat His Arg Val Asp Asn Giu Arg 900 acc aag ctg ggt cgg gga Thi- Lys Leu Giy Arg Gly 915 cag ttg ttg acc atg atg cat Gin Leu Leu Thr Met Met His 930 920 gag Giu 925 att ccg gag ctg Ile Pro Giu Leu 940 ctg gaa aag cat Leu Giu Lys His 955 gaa gcg tgg ctg Giu Ala Trp Leu 970 cag Gin acg Thr cac aat acg tcg acg His Asn Thr Ser Thr 945 att att aac cct gtg Ile Ilie Asn Pro Val 960 gca acg gct gct agg Ala Thr Ala Ala Arg ttg gaa gtt ctt Leu Giu Val Leu cag gtg cag acg Gin Val Gin Thr 965 aat gcg ctt gtg Asn Ala Leu Val 980 975 agg ggt Arg Giy 985 aag aga Lys Arg ctt gcg cag gtg gct ggt 3139 Leu Ala Gin Val Ala Gly 1000 gag tat ttg gaa. aac tat 3187 Giu Tyr Leu Glu Asn Tyr 1015 gtt gat gaa gtc ttc tgg 3235 Val Asp Giu Val Phe Ti-p tta gat Leu Asp gcg tct Ala Ser 100 ctg aaa Leu Lys 1020 ggt gtg cag tta cct act cct ggt ccg cac Gin Leu Pro Thr Pro Gly Pro'His 990 995 ggt tgg gat cca aat gag tac cag Gly Ti-p Asp Pro Asn Giu Tyr Gin 5 1010 gtg acc agg aag agt cgt cag gtt Val Thr Arg Lys Ser Arg Gin Val 1025 gac tct atg gag caa. cgt gag ttt Giy Val Asp Ser Met Giu Gin Arg Giu Phe 1030 1035 1040 1045 taggtaggtg gtgggagccc caa 3258 <210> 100 <211> 1045 <212> PRT <213> Corynebacterium giutainicum <400> 100 Met Ser Giy Pro Leu Arg Ser Giu Arg Lys Val Val Gly Phe Val Arg WO 01/00843 WO 0100843PCTAIBOO/00923 1 Asp Gin Leu Leu Arg Arg Val1 Arg Al a 145 Asp Glu Ile Lys Asp 225 Asn Met Val1 Ala Ala 305 Ser Pro Ala Leu Leu Asn Leu Ala G lu 130 Val Asp Ala Ala G ly 210 Leu Val1 Gly Val Glu 290 Leu His Leu Asp Trp Ile Glu Phe Asn 115 Glu Leu Leu Glu Ala 195 Gly Ala Tyr Lys Phe 275 Leu Arg Met Pro Leu Gly Arg Leu Al a 100 Pro Met Glu Ser Arg 180 His Asp Asp Gly Cys 260 Val1 Ile Pro Ala Gly Leu Gly Gln Glu Gly Trp, Ala 135 Asp Gly Lys Ala Pro 215 Leu Pro Gln Pro G ly 295 Lys Lys Ser Gly 40 Ala Ala Ile Gly Lys 120 Leu Phe Phe Trp Gly 200 Phe Thr Val Glu Ala 280 Ser Ser Arg Ser Arg Asp Glu Gin Ser Leu Glu Asp Thr 170 Tyr Tyr Thr Ala Ser 250 Asn Ser Ser Ala Ala 330 Leu Asn Pro Ala Asp Ala Lys Ser Ala 155 Al a Arg Pro Val Leu 235 Ala Tyr Lys Phe Leu 315 Glu Ser Glu Val1 Gly Lys Gly Asp 125 Lys Asn Val1 Leu Asp 205 Met Val Ser Ser Thr 285 Glu Arg Trp WO 01/00843 WO 0100843PCTJIIBOO/00923 Ala Ser Glu Asn 385 Gly Gly His Leu Leu 465 Met Glu Leu Asn Ala 54.5 Ala Lys Thr Ala Val1 625 Ser Leu Tyr Ser 370 Val1 Leu Arg Val1 Ile 450 Glu As n Gln Gln Ser 530 Ala Tyr Ile Ala Ser 610 Val1 Glu Pro S er Val1 375 Arg Phe Leu Gly Phe 455 Thr Ala Ala His Ser 535 Ala Ala Leu G ly Trp 615 Met Thr Met Pro 360 Gln Asp Ala Arg Tyr 440 Leu His Arg Met Ser 520 Ala Ala Leu Pro Leu 600 Phe Arg Pro Gly Ile Met Glu Gln 410 Arg Gly Leu Leu Ser 490 Arg Leu Ala Gly Ser 570 Leu Asn Arg Leu Phe 650 Asn Ala 365 Arg Leu Gln Val1 Asp 445 His Pro Thr Arg Arg 525 Leu His Ser Trp Lays 605 Arg Ser Gln Leu 350 Ser Val1 Gly Met Asn 430 Gly Arg Asp Gly Lys 510 Pro Ser Pro Arg Leu 590 Leu Asp Pro Leu Gly Gln Leu Arg Val1 415 Ala His Leu Asp Ser 495 Val Leu Pro Ser Lys 575 Ser Ser Glu Tyr Gly 655 Glri Arg Asp Gly 400 His Leu Asn Gln Arg 480 Met Arg Leu Asp Arg 560 Al a Gln Asp Gly Ile 640 Asp WO 01/00843 PCT/IB00/00923 Ala Ala Ser Gly Pro Lys Leu Leu Ala Thr Ala Pro Thr Gln Val Val 660 665 670 Lys Ala Ile Lys Ala Thr Val Ser Arg His Glu Ser Pro Asp Arg Ala 675 680 685 Ile Gin Ala Ala Arg Ser Leu Arg Arg Gin Glu Leu Ala Arg Ile Ala 690 695 700 Ser Ala Asp Leu Leu Asn Met Leu Thr Val Gin Glu Val Cys Gin Ser 705 710 715 720 Leu Ser Leu Val Trp Asp Ala Val Leu Asp Ala Ala Leu Asp Ala Glu 725 730 735 Ile Arg Ala Ala Leu Asn Asp Pro Gin Lys Pro Asp Gin Pro Leu Ala 740 745 750 Asn Ile Ser Val Ile Gly Met Gly Arg Leu Gly Gly Ala Glu Leu Gly 755 760 765 Tyr Gly Ser Asp Ala Asp Val Met Phe Val Cys Glu Pro Val Ala Gly 770 775 780 Val Glu Glu His Glu Ala Val Thr Trp Ser Ile Ala Ile Cys Asp Ser 785 790 795 800 Met Arg Ser Arg Leu Ala Gin Pro Ser Gly Asp Pro Pro Leu Glu Val 805 810 815 Asp Leu Gly Leu Arg Pro Glu Gly Arg Ser Gly Ala Ile Val Arg Thr 820 825 830 Val Asp Ser Tyr Val Lys Tyr Tyr Glu Lys Trp Gly Glu Thr Trp Glu 835 840 845 Ile Gin Ala Leu Leu Arg Ala Ala Trp Val Ala Gly Asp Arg Glu Leu 850 855 860 Gly Ile Lys Phe Leu Glu Ser Ile Asp Arg Phe Arg Tyr Pro Val Asp 865 870 875 880 Gly Ala Thr Gin Ala Gin Leu Arg Glu Val Arg Arg Ile Lys Ala Arg 885 890 895 Val Asp Asn Glu Arg Leu Pro Arg Gly Ala Asp Arg Asn Thr His Thr 900 905 910 Lys Leu Gly Arg Gly Ala Leu Thr Asp Ile Glu Trp Thr Val Gin Leu 915 920 925 Leu Thr Met Met His Ala His Glu Ile Pro Glu Leu His Asn Thr Ser 930 935 940 Thr Leu Glu Val Leu Glu Val Leu Glu Lys His Gin Ile Ile Asn Pro 945 950 955 960 Val Gin Val Gin Thr Leu Arg Glu Ala Trp Leu Thr Ala Thr Ala Ala 965 970 975 Arg Asn Ala Leu Val Leu Val Arg Gly Lys Arg Leu Asp Gin Leu Pro WO 01/00843 WO 0100843PCTIiBOO/00923 990 Ala Gly Ala Ser Gly Trp Asp 1005 Thr Pro Gly 995 Pro Asn Glu 1010 Lys Ser Arg 1025 Pro His Leu Ala Gin Val 1000 Tyr Gin Giu Tyr Leu Giu 1015 Gin Val Val Asp Glu Vai 1030 Asn Tyr Leu Lys 1020 Phe Trp Gly Val 1035 Val Thr Arg Asp Ser Met 1040 Giu Gin Arg Glu Phe 1045 <210> 101 <211> 861 <212> DNA <213> Corynebacterium giutamicun <22 0> <221> CDS <222> <223> RXN03176 <400> 101 gag ttg g( Glu Leu A: 1 cc gat tac la Asp Tyr 5 atc ccg gaa cta Ile Pro Glu Leu tct gcg gac cca Ser Ala Asp Pro aac ccg Asn Pro ctg gca gta Leu Ala Val gat gac gac Asp Asp Asp ctg tgc acc gtt Leu Cys Thr Val gga cac atc tac Gly His Ile Tyr agc gca ggc Ser Ala Gly cca ttt gcc Pro Phe Ala atc gaa ttc acc atg caa agt att tcc Ile Glu Phe Thr met Gin Ser Ile Ser 40 aag Lys tac gca Tyr Ala ctc gca ctc caa gaa tgc ggc ttt gat Leu Ala Leu Gin Giu Cys Gly Phe Asp gtc tct gca tcc Val Ser Ala Ser gtg Val1 gcc ttg gag ccc Ala Leu Giu Pro ggt gag gcc ttc Gly Giu Ala Phe gaa ctt tcc ctc Giu Leu Ser Leu ggc gaa aac cgc Gly Glu Asn Arg atg aac ccc atg Met Asn Pro Met aac gcc ggc gcg Asn Aia Gly Ala atc gcc Ile Ala atc aac cag Ilie Asn Gin gaa aaa atc Glu Lys Ile 115 atc aac ggc tcc gat tcc acc gtg gaa Ile Asn Gly Ser Asp Ser Thr Val Giu 105 gac cgc gtg Asp Arg Val 110 gaa ctc acc Giu Leu Thr cga cac tac ttc Arg His Tyr Phe gaa ctt gct gga Giu Leu Ala Gly atc gac Ile ASP 130 cgc gtg ctt gcc Arg Val Leu Aia gaa Giu 135 tcc gaa ctc gcc Ser Giu Leu Ala gcc gac cgc aac Ala Asp Arg Asn 432 WO 01/00843 WO 0100843PCTIBOOIOO923 ctc tcc atc gcc cac atg ctg cgc aat tac ggc gtc atc gaa gac gaa 480 Leu Ser Ile Ala His Met Leu Arg Asn Tyr Gly Val Ile Giu Asp Giu 145 150 155 160 gcc cac gac gcc gtc ctc agc tac acg ctg caa tgc gcc atc aaa gta 528 Ala His Asp Ala Val Leu Ser Tyr Thr Leu Gin Cys Ala Ile Lys Val 165 170 175 acc acg cgc gac ctc gca gtc atg acc gcc acg ctc gcc gcc ggc ggc 576 Thr Thr Arg Asp Leu Ala Val Met Thr Ala Thr Leu Ala Ala Gly Gly 180 185 190 aca cac cca att acc ggc aag aag ctt ctc gac gcc cgc gtc tgc cgc 624 Thr His Pro Ile Thr Giy Lys Lys Leu Leu Asp Ala Arg Vai Cys Arg 195 200 205 ctc acc ctc tcc gtc atg gct tca gca ggc atg tac gac gag gca ggg 672 Leu Thr Leu Ser Val Met Ala Ser Ala Gly Met Tyr Asp Glu Ala Gly 210 215 220 cag tgg ctc tcc acc gta ggc atc ccc gcg aaa tca gga gtc gcc ggc 720 Gin Trp Leu Ser Thr Val Gly Ile Pro Ala Lys Ser Giy Val Ala Gly 225 230 235 240 gga ctc atc ggc att ctg cca ggt cag ctg ggc atc gcc aca ttt tcc 768 Gly Leu Ilie Gly Ile Leu Pro Giy Gin Leu Giy Ile Ala Thr Phe Ser 245 250 255 cca cgc ctg aac ccc aaa ggc aac agc gtg cgc ggc gta aaa ata ttc 816 Pro Arg Leu Asn Pro Lys Gly Asn Ser Vai Arg Giy Vai Lys Ile Phe 260 265 270 aaa. cag ctt tcc gac gac atg ggc ctc cac ctc atg tcc acc gag 861 Lys Gin Leu Ser Asp Asp Met Gly Leu His Leu Met Ser Thr Giu 275 280 285 <210> 102 <211> 287 <212> PRT <213> Corynebacterium giutamicun <400> 102 Giu Leu Ala Asp Tyr Ilie Pro Glu Leu Lys Ser Ala Asp Pro Asn Pro 1 5 10 Leu Ala Val Ala Leu Cys Thr Vai Asn Giy His Ilie Tyr Ser Ala Gly 25 Asp Asp Asp Ile Giu Phe Thr Met Gin Ser Ile Ser Lys Pro Phe Ala 40 Tyr Ala Leu Ala Leu Gin Giu Cys Gly Phe Asp Giu Val Ser Ala Ser 55 Val Ala Leu Giu Pro Ser Gly Giu Ala Phe Asn Giu Leu Ser Leu Asp 70 75 Gly Giu Asn Arg Pro Met Asn Pro Met Ile Asn Ala Gly Ala Ile Ala 90 WO 01/00843 WO 0100843PCTJLBOOIOO923 Ile Giu Ile Leu 145 Ala Thr Thr Leu Gin 225 Gly Pro Lys Gin Ile 115 Arg Ile Asp Arg Pro 195 Leu Leu Ile Leu Leu 275 Leu 100 Arg Val Ala Ala Asp 180 Ile Ser Ser Gly Asn 260 Ser Asn Tyr Ala Met 150 Leu Ala Gly Met Val1 230 Leu Lys Asp Gly Phe Giu 135 Leu Ser Val1 Lys Ala 215 Gly Pro Gly Met Se r Ser 120 Ser Arg Tyr Met Lys 200 Ser Ile Gly Asn G iy 280 Val G ly Giy 140 Val1 Cys Leu Ala Tyr 220 Ser Ile Gly Met Giu Arg 125 Ala Ile Ala Ala Arg 205 Asp Gly Ala Val1 S er 285 Asp 110 Giu Asp Glu Ile Ala 190 Val1 Glu Val1 Thr Lys 270 Thr Val1 Thr Asn Giu 160 Val1 Gly Arg Gly Gly 240 Ser Phe <210> 103 <211> 861 <212> DNA <213> Cory-nebacteriun glutamicun <2 <221> CDS <222> <223> FRXA02879 <400> 103 gag ttg gcc gat tac atc ccg gaa cta Glu Leu Ala Asp Tyr Ile Pro Giu Leu 1 5 ctg gca gta gcc ctg tgc acc gtt aac Leu Ala Val Ala Leu Cys Thr Val Asn 25 gat gac gac atc gaa ttc acc atg caa Asp Asp Asp Ile Giu Phe Thr Met Gin 40 aaa tct gcg gac cca aac ccg 48 Lys Ser Ala Asp Pro Asn Pro 10 gga cac atc tac agc gca ggc 96 Gly His Ile Tyr Ser Ala Gly agt att tcc aag cca ttt gcc 144 Ser Ile Ser Lys Pro Phe Ala WO 01/00843 tac gca ctc Tyr Ala Leu gtg gcc ttg Val Ala Leu ggc gaa aac Giy Glu Asn atc aac cag Ile Asn Gin gaa aaa atc Giu Lys Ile 115 atc gac cgc Ile Asp Arg 130 ctc tcc atc Leu Ser Ile 145 gcc cac gac Aia His Asp acc acg cgc Thr Thr Arg aca cac cca Thr His Pro 195 ctc acc ctc Leu Thr Leu 210 cag tgg ctc Gin Trp, Leu 225 gga ctc atc Gly Leu Ile cca cgc ctg Pro Arg Leu aaa cag ctt Lys Gin Leu 275 tgc Cys gag Giu ccc Pro tcc Ser tc t Ser 120 tcc Ser cgc Arg tac Tyr atg Met aag Lys 200 tca Ser atc Ile gg t Giy aac Asn ggc Gly 280 ggc Gly gcc Aia a tg Met gat Asp 105 gaa Glu gaa Giu aat Asn acg Thr acc Thr 185 ctt Leu gca Ala ccc Pro cag Gin agc Ser 265 ctc Leu ttt Phe t tc Phe atc Ile tcc Ser ctt Leu ctc Leu tac Tyr c tg Leu 170 gcc Ala C tc Leu ggc Giy gcg Ala ctg Leu 250 g tg Val1 cac His gtc Val ctt Leu ggc Gly gaa Giu cgc Arg 125 gcc Ala atc Ile gcc Ala gcc Ala cgc Arg 205 gac Asp gga Gly gcc Ala gta Val1 tcc Ser 285 tct Ser tcc Ser gcg Ala gac Asp 110 gaa Giu gac Asp gaa Giu atc Ile gcc Ala 190 gtc Val1 gag Giu gtc Val aca Thr aaa Lys 270 ac c Thr PCTJIBOO/00923 gca tcc 192 Ala Ser ctc gac 240 Leu Asp atc gcc 288 Ile Ala cgc gtg 336 Arg Val ctc acc 384 Leu Thr cgc aac 432 Arg Asn gac gaa 480 Asp Giu 160 aaa gta 528 Lys Vai 175 ggc ggc 576 Gly Gly tgc cgc 624 Cys Arg gca ggg 672 Ala Gly gcc ggc 720 Ala Gly 240 ttt tcc 768 Phe Ser 255 ata ttc 816 Ile Phe gag 861 Glu WO 01/00843 PCT/IB00/00923 <210> 104 <211> 287 <212> PRT <213> Corynebacterium glutamicum <400> 104 Glu Leu Ala Asp Tyr Ile Pro Glu Leu Lys Ser Ala Asp Pro Asn Pro 1 5 10 Leu Ala Val Ala Leu Cys Thr Val Asn Gly His Ile Tyr Ser Ala Gly 25 Asp Asp Asp Ile Glu Phe Thr Met Gin Ser Ile Ser Lys Pro Phe Ala 40 Tyr Ala Leu Ala Leu Gin Glu Cys Gly Phe Asp Glu Val Ser Ala Ser 55 Val Ala Leu Glu Pro Ser Gly Glu Ala Phe Asn Glu Leu Ser Leu Asp 70 75 Gly Glu Asn Arg Pro Met Asn Pro Met Ile Asn Ala Gly Ala Ile Ala 90 Ile Asn Gin Leu Ile Asn Gly Ser Asp Ser Thr Val Glu Asp Arg Val 100 105 110 Glu Lys Ile Arg His Tyr Phe Ser Glu Leu Ala Gly Arg Glu Leu Thr 115 120 125 Ile Asp Arg Val Leu Ala Glu Ser Glu Leu Ala Gly Ala Asp Arg Asn 130 135 140 Leu Ser Ile Ala His Met Leu Arg Asn Tyr Gly Val Ile Glu Asp Glu 145 150 155 160 Ala His Asp Ala Val Leu Ser Tyr Thr Leu Gin Cys Ala Ile Lys Val 165 170 175 Thr Thr Arg Asp Leu Ala Val Met Thr Ala Thr Leu Ala Ala Gly Gly 180 185 190 Thr His Pro Ile Thr Gly Lys Lys Leu Leu Asp Ala Arg Val Cys Arg 195 200 205 Leu Thr Leu Ser Val Met Ala Ser Ala Gly Met Tyr Asp Glu Ala Gly 210 215 220 Gin Trp Leu Ser Thr Val Gly Ile Pro Ala Lys Ser Gly Val Ala Gly 225 230 235 240 Gly Leu Ile Gly Ile Leu Pro Gly Gin Leu Gly Ile Ala Thr Phe Ser 245 250 255 Pro Arg Leu Asn Pro Lys Gly Asn Ser Val Arg Gly Val Lys Ile Phe 260 265 270 Lys Gin Leu Ser Asp Asp Met Gly Leu His Leu Met Ser Thr Glu 275 280 285 WO 01/00843 WO 0100843PCTIBOO/00923 <210> 105 <211> 1155 <212> DNA <213> Corynebacterium glutamicum <220> <221> <222> <223>

CDS

(101)..(1132) RXA0 0278 <400> 105 ggccacggag ttggtgtccg tcatggtgat gattctgtct gtcgtttctg tgttggcttt gtgggtcggc atccgccccc gtttgcagga gtacttataa a tg Met 1 cac gct ttt His Ala Phe cgc ccc cct cca ctc acc acg cga gtc Arg Pro Pro Pro Leu Thr Thr Arg Val gct gca ttg ctg Ala Ala Leu Leu gcc gca Ala Ala acg ctg ctt Thr Leu Leu ttg act gct Leu Thr Ala tcc tgc act cca Ser Cys Thr Pro cct gtg gaa ccg gca gaa acc Pro Val Glu Pro Ala Glu Thr ttg gat ccc gat Leu Asp Pro Asp ggt cca cca ctg Gly Pro Pro Leu ccg gat tct Pro Asp Ser tcg att Ser Ile gaa gct ccc ggt Glu Ala Pro Gly gaa Glu aaa gag ccc att Lys Glu Pro Ile gaa gta ata gag Glu Val Ile Glu aat Asn tgg cca ggt tct Trp Pro Gly Ser cgc ccg gat gat Arg Pro Asp Asp c tg Leu acc cct gag gag Thr Pro Glu Glu gta cct ggc atc Val Pro Gly Ile aac cgg ggt cgc Asn Arg Gly Arg att gtg ggt gtg Ile Val Gly Val gat caa Asp Gln 100 tcg caa aac Ser Gln Asn ggt ttt gaa Gly Phe Glu 120 t tg Leu 105 ctc agt ttc cgt Leu Ser Phe Arg ccg gtg act ggt Pro Val Thr Gly gag ctg cgc Glu Leu Arg 115 att ttc ggt Ile Phe Gly gtg gaa tta gcg Val Glu Leu Ala gaa att tcc cgc Glu Ile Ser Arg gac Asp 130 gac ccc Asp Pro 135 aat aag gtg gat Asn Lys Val Asp t tC Phe 140 cga ttc gtc ggc Arg Phe Val Gly tcg Ser 145 tcc gac cgt ctg Ser Asp Arg Leu cgt Arg 150 tcc ctt gac caa Ser Leu Asp Gln gat gta gat att Asp Val Asp Ile gtg Val1 160 att cgt tcC gtC Ile Arg Ser Val atc acc gac gaa Ile Thr Asp Glu gcc aaa ttg gtg Ala Lys Leu Val ttt tcc aca ccg Phe Ser Thr Pro tac ctg Tyr Leu 180 WO 01/00843 cgc acc ca Arg Thr Gi atc gca ga Ilie Ala As tca ttg ca Ser Leu GI 215 act cgc aa Thr Arg As 230 cag gtc at Gin Vai Il gat ccc ta Asp Pro Ty gga gtg gc Gly Val Al 28 att cgg ca 1027 Ilie Arg Gl 295 tgg t99 ac 1075 Trp Trp T1h 310 ggt cca cc 1123 Gly Pro Pr gat gaa gg 1155 Asp Giu G1 <210> 106 <211> 344 <212> PRT a acc cgc *n Thr Arg 185 Lt cta ccc p Leu Pro 10 ~g cga gcc ,n Arg Ala Lt tgg tcc n Trp Ser .t ttg ggc .e Leu Gly 250 Lc acc gag 'r Thr Giu 265 a gcg gca .a Ala Ala 10 ~g gpa aac .n Val Asn a atg ttc ~r Met Phe :a cag ctg o Gin Leu 330 ra taatgaac t tg Leu cac His acc Thr 220 tgt Cys gat Asp ctt Leu acc Thr ac a Thr 300 gat Asp tac acc Thr acc Thr 205 at t Ile ctc Leu gtc Val1 ga t Asp act Thr 285 att Ile tgg Trp a tg atg gaa tct Met Giu Ser 190 att tgt gtc Ile Cys Val gcg ccg gag Ala Pro Giu atg gcg ttg Met Ala Leu 240 att ttg tcc Ile Leu Ser 255 acc tcc ctc Thr Ser Leu 270 gct gaa aca Ala Giu Thr gaa cgg atc Giu Arg Ile ttc gga cct Phe Gly Pro 320 cca gag gaa Pro Giu Glu gga Gly ga t Asp 210 tca Ser cag Gin atc Ile tcc Ser tct Ser 290 aca Thr c tc Leu ggg Gly atc Ile 195 ggc Gly atc Ile cat His gca Ala cat His 275 tcg Ser gac Asp tgg Trp aca Thr PCTIBOOIOO923 acg tcc 691 Thr Ser tcc act 739 Ser Thr tta aaa 787.

Leu Lys cag gct 835 Gin Ala 245 gca cag 883 Ala Gin 260 tcc tat 931 Ser Tyr ggg ttg 979 Gly Leu cgc atg Arg Met tcc tac Ser Tyr 325 gaa aac Giu Asn 340 Gin Tyr Met gat ttcgatccag att <213> Corynebacterium giutainicum <400> 106 met His Ala Phe Arg Arg Pro Pro Pro Leu Thr Thr Arg Val Gly Ala 1 5 10 Ala Leu Leu Ala Ala Thr Leu Leu Ala Ser Cys Thr Pro Thr Pro Val 25 WO 01/00843 PCT/IB00/00923 Glu Pro Ala Glu Thr Leu Thr Ala Leu Asp Pro Asp Ala Gly Pro Pro 40 Leu Pro Pro Asp Ser Ser Ile Glu Ala Pro Gly Glu Lys Glu Pro Ile 55 Val Glu Val Ile Glu Asn Trp Pro Gly Ser Leu Arg Pro Asp Asp Leu 70 75 Thr Pro Glu Glu Arg Val Pro Gly Ile Val Asn Arg Gly Arg Ile Ile 90 Val Gly Val Asp Gin Ser Gln Asn Leu Leu Ser Phe Arg Asp Pro Val 100 105 110 Thr Gly Glu Leu Arg Gly Phe Glu Val Glu Leu Ala Arg Glu Ile Ser 115 120 125 Arg Asp Ile Phe Gly Asp Pro Asn Lys Val Asp Phe Arg Phe Val Gly 130 135 140 Ser Ser Asp Arg Leu Arg Ser Leu Asp Gin Gly Asp Val Asp Ile Val 145 150 155 160 Ile Arg Ser Val Thr Ile Thr Asp Glu Arg Ala Lys Leu Val Glu Phe 165 170 175 Ser Thr Pro Tyr Leu Arg Thr Gin Thr Arg Met Leu Thr Met Glu Ser 180 185 190 Ser Gly Ile Thr Ser Ile Ala Asp Leu Pro Gly His Thr Ile Cys Val 195 200 205 Thr Asp Gly Ser Thr Ser Leu Gin Arg Ala Arg Thr Ile Ala Pro Glu 210 215 220 Ala Ser Ile Leu Lys Thr Arg Asn Trp Ser Asp Cys Leu Met Ala Leu 225 230 235 240 Gin Gin His Gin Ala Gin Val Ile Leu Gly Asp Asp Val Ile Leu Ser 245 250 255 Gly Ile Ala Ala Gin Asp Pro Tyr Thr Glu Ile Leu Asp Thr Ser Leu 260 265 270 Asp Ser His Ser Tyr Gly Val Ala Ala Ala Ser Thr Thr Ala Glu Thr 275 280 285 Asp Ser Ser Gly Leu Ile Arg Gin Val Asn Tyr Thr Ile Glu Arg Ile 290 295 300 Arg Thr Asp Arg Met Trp Trp Thr Met Phe Asp Asp Trp Phe Gly Pro 305 310 315 320 Tyr Leu Trp Ser Tyr Gly Pro Pro Gin Leu Gln Tyr Met Pro Glu Glu 325 330 335 Glu Gly Thr Glu Asn Asp Glu Gly 340 WO 01/00843 WO 0100843PCT/IBOO/00923 <210> <211> <212> <213> <220> <221> <222> <223> 107 1035

DNA

Corynebacteriun

CDS

(101)..(1012) R.XA0 0727 giutaniicum <400> 107 ttattgcacc atggcaggta gtattttgcc catgattgag agtttcaccc gattgcgaat ttcgcagaca ccgcctctga aacggctact gcatagcact ttg cgt ttc ccc Leu Arg Phe Pro atc ccc aag cgc gct gta gcg gcg acc Ile Pro Lys Arg Ala Val Ala Ala Thr ggc atc gtg gca Gly Ile Val Ala acc tca Thr Ser ttc acc ttg Phe Thr Leu tct tgt gtc ac Ser Cys Val Thr aa t Asn gag gag cag ggc aac cca gat Giu Giu Gin Gly Asn Pro Asp ggc tgg gag cag atc gtt cca Gly Trp Giu Gin Ile Val Pro cct gta ccg gag Pro Val Pro Giu cag gcg atg Gin Ala Met gtt ccc Val Pro gaa gct ctg gct Giu Ala Leu Ala cgc ggt gtg ctc Arg Gly Val Leu gcc ggt gcc aac Ala Gly Ala Asn cca Pro cct ttc cca ccg Pro Phe Pro Pro ttt Phe gag ttt aaa gat Giu Phe Lys Asp gat ggt cag att Asp Giy Gin Ile ggt gtg gaa atg Gly Val Giu Met ctc gtg cgt gca Leu Val Arg Ala gcg ggg gtg atg Ala Gly Val Met ggc ttg Gly Leu 100 gag ttc agc Giu Phe Ser caa gct ggc Gin Ala Gly 120 cc t Pro 105 cag gag cag gat Gin Giu Gin Asp tcc ctc atc ctt Ser Leu Ile Leu cca tcg gtt Pro Ser Val 115 gac aac gag Asp Asn Giu acc ctt gat atc Thr Leu Asp Ilie gcc tct ggc ttc Ala Ser Giy Phe act Thr 130 gaa cgc Giu Arg 135 cgc gag aac ttt Arg Giu Asn Phe ga t Asp 140 ttc atc gat ttc Phe Ile Asp Phe ttc gca ggt gtg Phe Ala Gly Val cag Gin 150 tgg gcg cag gca Trp Ala Gin Ala act Thr 155 gat cgt gaa acc Asp Arg Giu Thr atc gat ccg gaa Ile Asp Pro Giu gcc tgt ggt ctc Ala Cys Gly Leu gtt gct gta cag Val Ala Val Gin cgc Arg 175 aca acc gtg gca Thr Thr Val Ala gag acc Giu Thr 180 WO 01/00843 gac gat gtc cgt cct cgc tca gct caa tgt gaa Asp Asp Val Arg Pro Arg Ser Ala Gin Cys Glu 185 190 ccg atc acc att ttg tct tat gaa acc gca gat Pro Ilie Thr Ile Leu Ser Tyr Giu Thr Ala Asp 200 205 ttg atc ctg gga cgc gca gac gca ctt gct gcg Leu Ile Leu Gly Arg Ala Asp Ala Leu Ala Ala .215 220 gct tgg gct gca gag cgc tcc gaa ggc cgc atc Ala Trp Aia Ala Giu Arg Ser Glu Gly Arg Ile 230 235 240 atg tac ttg gct gcg cca ttt ggt ttc gca ttc Met Tyr Leu Ala Ala Pro Phe Gly Phe Ala Phe 250 255 ctc acc cca gca gca gct gcg gcg ttc caa cac Leu Thr Pro Ala Ala Ala Ala Ala Phe Gin His 265 270 gat tac cag cgc atc atg gcg caa tgg ggc att Asp Tyr Gin Arg Ile Met Ala Gin Trp, Gly Ile 280 285 gat gag gcc ctg atc aac gaa cag cca ctc aac 1032 Asp Glu Ala Leu Ile Asn Glu Gin Pro Leu Asn 295 300 aaa 1035 <210> 108 <211> 304 <212> PRT <213> Corynebacteriun giutamicum <400> 108 Leu Arg Phe Pro Lys Ile Pro Lys Arg Ala Val 1 5 Ilie Val Ala Thr Ser Phe Thr Leu Ala Ser Cys Gin Gly Asn Pro Asp Gly Trp Giu Gin Ilie Val Glu Ile Gin Ala Met Val Pro Glu Ala Leu Ala 55 Thr Ala Gly Ala Asn Pro Pro Phe Pro Pro Phe 70 Asp Gly Gin Ilie Ile Gly Val Glu Met Asp Leu gcc gaa Ala Giu act gca Thr Ala 210 gac tcc Asp Ser 225 gaa gtt Giu Val ccg ttg Pro Leu ttg att Leu Ile gaa gaa Glu Giu 290 PCTIBOOIOO923 ggc aaa gag 691 Gly Lys Glu 195 gct acc gca 739 Ala Thr Ala cct gtt tca 787 Pro Val Ser gtg ggc gat 835 Val Gly ASP 245 gaa tct gac 883 Glu Ser Asp 260 gac acc ggc 931 Asp Thr Gly 275 ggc ctt ctt 979 Gly Leu Leu tagagccttc cagcaactaa Ala Val1 Pro Gin Giu Val1 WO 01/00843 Gly Ile Phe Leu 145 Ilie Thr Ala Thr Asp 225 Glu Pro Leu Glu Val1 Leu Thr 130 Phe Asp Val Giu Ala 210 Ser Val Leu Ile Glu 290 Met Pro 115 Asp Ala Pro Ala Gly 195 Al a Pro Val1 Glu Asp 275 Gly Giu Gin Giu Gin 150 Ala Asp Pro Leu Al a 230 Met Leu Asp Asp Phe Ala Arg 135 Trp Cys Asp Ile Ile 215 Trp Tyr Thr Tyr Glu 295 Ser Gly 120 Arg Ala Gly Val Thr 200 Leu Ala Leu Pro Gin 280 Ala Pro 105 Thr Giu Gin Leu Arg 185 Ile Gly Ala Ala Ala 265 Aig Leu Glu Asp Phe Thr 155 Val Arg Ser Ala Arg 235 Pro Ala Met Asn Asp Gly 125 Phe Arg Val1 Ala Glu 205 Ala Giu Gly Ala Gin 285 Gin Phe 110 Ala Ile Glu Gin Gin 190 Thr Leu Gly Phe Phe 270 Trp Pro PCT/1B00100923 Ser Leu Ser Gly Asp Phe Thr Pro 160 Arg Thr 175 Cys Giu Ala Asp Ala Ala Arg Ile 240 Ala Phe 255 Gin His Gly Ilie Leu Asn <210> 109 <2 11> 1962 <212> DN'A <213> Corynebacteriun giutainicum <220> <221> CDS <222> (101)..(1939).

<223> RXA02139 <400> 109 tcaggaggat ttttcaatca tgtgcggcct tcttggcata ttgactgcaa atgggaacgc tgaagcattc gttcctgcac tcgagcgggc cttgccatgc atg cgc cac cgt ggt 115 Met Arg His Arg Gly 1 cct gac gat gcc ggc act tgg cat gac gcc gat gca gcg ttt gga ttc 163 169 WO 01/00843 WO 0100843PCT/IBOO/00923 Pro a ac Asn tgg Trp gag Giu tat Tyr cac Hius att Ile cag Gin gtg Vai aa t Asn 150 t tg Leu t tg Leu cag Gin gg t Giy agc Ser 230 Asp cgc Arg gga G iy atc Ilie gcc Aia cac His gcc Aia t tc Phe ttc Phe 135 cta Leu cag Gin gag Giu aag Lys aag Lys 215 gtc Val Asp Ala Giy ctc tcc atc Leu Ser Ile cct gcg gat Pro Aia Asp tac aac tac Tyr Asn Tyr ttt aat act Phe Asn Thr tgg ggc gag Trp Giy Giu att tgg gat Ilie Trp Asp 105 ggc att aag Giy Ilie Lys 120 tcc tca gag Ser Ser Giu gat ctg ggc Asp Leu Gly tac gtg ccc Tyr Vai Pro 170 tca ggc tgc Ser Gly Cys 185 cgt tac ttc Arg Tyr Phe 200 gag cag gac Giu Gin Asp gaa aag cat Giu Lys His Thr Trp, His Asp Ala Asp Ala Ala Phe att Ile gaa Glu gtt Vai tc t Ser 75 tcc Ser aca Thr cca Pro aag Lys ctt Leu 155 gag Glu acc Thr aag Lys ctc Leu atg Met 235 gat Asp ccc Pro gag Glu 60 ggc Giy g tg Val aag Lys c tg Leu aag Lys 140 gat Asp cca Pro gca Ala cct Pro ttc Phe 220 cg t Arg att Ilie gac Asp 45 c tg Leu gat

ASP

gtc Val1 gaa Giu ttC Phe i2 5 acc Thr aag Lys ga t Asp aca Thr cag Gin 205 gat Asp gcc Ala gca Ala cgc Arg cgt Arg ggc Gly gag Glu aag Lys 110 tac TIyr atc Ile cgc Arg acc Thr gtt Vai 190 t tc Phe cgc Arg gac Asp att Ile cac His tac Tyr aaa Lys gag Giu cat His 95 tcg Ser gca Ala ttg Leu acc Thr ctt Leu 175 cgt Arg cca Pro att Ilie gtg Val1 gcg Al a tcc Ser gca Ala gag Giu cc a Pro 80 ctc Leu ctt Leu acc Thr gag Glu att Ile 160 c ac His ccg Pro gta Val gcc Ala acc Thr 240 gcg Ala c ac His atg Met ctc Leu att Ile cgc Arg t tc Phe acc Thr atg Met 145 gag Glu gcg Ala ggc Gly cag Gin cag Gin 225 gta Val ctt Leu caa Gin act Thr tcg Ser gtt Val1 gga Gly ctt Leu gag Giu 130 gcc Ala cac His cag Gin Gly aag Lys 210 gtg Val1 9gc Gly gc a Ala Gly c tg Leu aac Asn t tg Leu ggt Giy ttc Phe 100 cgt Arg ggc G ly gag Glu gtg Val tcc Ser 180 c tg Leu gta Val gag Glu ttc Phe cgc Arg Phe cg t Arg gg t Gly gga Gly t tc Phe ggc Gly gat Asp acc Thr atg Met gac Asp 165 cgc Arg gaa Glu aag Lys ga t Asp ctt Leu 245 cac His ttc ggc ggc Phe Gly Gly att gac tca acc gca Ile Asp Ser Thr Ala WO 01/00843 WO 0100843PCTAIBOO/00923 255 gg t Gly 260 ggc tac Gly Tyr aac cct gac Asn Pro Asp tcg gag gtc Ser Giu Val 280 acc ttc acc Thr Phe Thr ttc gag cgt Phe Giu Arg gaa Glu 275 gtg gct gcg Val Ala Ala cac atc 1027 His Ile 295 aag atc 1075 Lys Ile 310 ccg ctg 1123 Pro Leu ctg tct 1171 Leu Ser aag gag 1219 Lys Glu cgt aaa 1267 Arg Lys 375 ggc aag 1315 Gly Lys 390 tac ggc 1363 Tyr Gly cca tgg 1411 Pro Trp gtg aag att gtC tcg Val Lys Ile Val Ser 300 atg tgg tac ttg gat Met Trp Tyr Leu Asp 315 tac ttc gtg gca gcg Tyr Phe Val Ala Ala gag Giu 285 cc t Pro ga t Asp gcc gct gcg Ala Ala Ala att ggc gct gag Ile Gly Ala Giu 290 aac gcg att cct Asn Ala Ile Pro 979 gag gaa tac Glu Glu Tyr cct gta gct Pro Val Ala gcc Ala 305 320 gaa gca cgt Glu Ala Arg 335 aag Lys gac cca tca ttg gtc Asp Pro Ser Leu Val 325 cac gtc aag gtt gtg His Val Lys Val Val 340 gga tac acc att tac Gly Tyr Thr Ile Tyr 330 ggc gag Gly Glu 345 ccg cta Pro Leu ggc gca gat gag ctg Gly Ala Asp Glu Leu 350 tcg ctt gct cca ttt Ser Leu Ala Pro Phe 365 ttc ggt Phe Gly 355 gag aag atc Glu Lys Ile ctg gga aag Leu Gly Lys tcc ctt ctt gag Ser Leu Leu Giu 395 aac gct cgc tcc Asn Ala Arg Ser ctc agc aag gtt ctg cca Leu Ser Lys Val Leu Pro 380 385 cgt ggc tcc atg acc atg Arg Gly Ser Met Thr Met 400 ttc aat ttc gag cag atg Phe Asn Phe Glu Gin Met 415 tgg gac cac cgc gaa gtc Trp, Asp His Arg Giu Val cct tcc cca cta Pro Ser Pro Leu 370 gac ggc atg aag Asp Gly Met Lys gaa gag cgc tac Glu Glu Arg Tyr 405 caa cgc gtt att Gin Arg Val Ile gca aag Ala Lys 425 cag tcc gaa Glu 420 act gcg ccg atc Thr Ala Pro Ile 435 atg caa cac ctg 430 tac gca 1459 cgc aac ttt gat cca gta gcc cgc Tyr Ala Gin Ser Arg Asn Phe 440 Asp 445 Pro Val Ala Arg Met Gin His Leu 450 WO 01/00843 WO 0100843PCTL-BOOIOO923 gat ctg 1507 Asp Leu 455 atc aac 1555 Ile Asn 470 gaa gtt 1603 Giu Val aac ggt 1651 Asn Gly ccg cct 1699 Pro Pro cgc cac 1747 Arg His 535 atc aag 1795 Ile Lys 550 gat atg 1843 Asp Met ctg tgg 1891 Leu Trp, aac cgc 1939 Asn Arg ttc acc tgg atg cgc Phe Thr Trp Met Arg 460 atg gcg aac tcc ctt Met Ala Asn Ser Leu 475 ttc aag gtt gca gag Phe Lys Val Ala Giu 490 acc acc aag tac gcg Thr Thr Lys Tyr Ala ggc gac atc ctg gtc aag gct gac aag Gly Asp Ile Leu Val Lys Ala Asp Lys 465 gag ctg cga gtt cca ttc ttg gat aag Giu Leu Arg Val Pro Phe Leu Asp Lys 480 485 acc att cct Thr Ile Pro 495 ctg cgc agg Leu Arg Arg 510 aag aag ctg Lys Lys Leu tac gac ctg aag att gcc Tyr Asp Leu Lys Ile Ala 500 gca ctc gag cag att gtt Ala Leu Glu Gin Ile Val 515 ggc ttc cct gtt ccc atg Gly Phe Pro Vai Pro Met 505 gtt ttg cac cgc Val Leu His Arg tgg ctt gcc ggc Trp Leu Ala Gly gaa tcc ggt act Giu Ser Gly Thr 555 ctg aac gag cac Leu Asn Giu His 570 gat Asp 540 525 gag Giu ctg ttc ggt tgg Leu Phe Giy Trp, 545 530 gcg cag gac acc Ala Gin Asp Thr cag gct gtg ctg Gin Ala Val Leu 565 gaa gat atc Glu Asp Ile cgc gat ggc Arg Asp Gly ttc aac Phe Asn 560 aag Lys act gtt ctg tca ttt atg gtg Thr Val Leu Ser Phe Met Vai 585 590 att gat cca cag att gag gac Ile Asp Pro Gin Ile Giu Asp 600 605 gtg tca gat cat tcc cgt cga Val Ser Asp His Ser Arg Arg 575 580 tgg cac ggc att ttt gtg gaa Trp His Gly Ile Phe Val Giu 595 cgc tcc tac cca gtc gag ctt Arg Ser Tyr Pro Val Giu Leu 610 taagtcttaa agcctaaacc ccc 1962 <210> 110 <211> 613 <212> PRT <213> Corynebacterium giutanicun <400> 110 Met Arg His Arg Giy Pro Asp Asp Ala Giy Thr Trp His Asp Ala Asp WO 01/00843 1 Ala Ala Phe His Gin Pro Met Thr Phe Leu Ser Asp Ile Val Val Arg Gly Met Phe Leu Ala 115 Thr Giu His 130 Met Ala Giu 145 Giu His Tyr Ala Gin Ile Gly Gly Lys 195 Gin Lys Val 210 Gin Vai Leu 225 Val Gly Ser Leu Ala Lys Giu Arg Glu 275 Ala Ile Gly 290 Ala Asn Ala 305 Asp Pro Ser PCTTBOO/00923 Gly Leu As n Leu Gly Phe 100 Arg Gly Giu Val1 Ser 180 Leu Val1 Giu Phe Arg 260 Gly Ala Ile Leu Phe Arg Gly Gly Phe Gly Asp Thr Met Asp 165 Arg Giu Lys Asp Leu 245 His Tyr Giu Pro Val 325 Arg Gly Ile 55 Ala His Ala Phe Phe 135 Leu Gin Giu Lys Lys 215 Val1 Gly Pro Giu Ile 295 Ile Leu Leu Pro 40 Tyr Phe Trp Ile Gly 120 Ser Asp Tyr Ser Arg 200 Giu Giu Gly Asp Val 280 Val Met Tyr Ser 25 Ala Asn Asn Gly Trp 105 Ile Ser Leu Val1 G ly 185 Tyr Gin Lys Ile Leu 265 Asp Lys Trp Phe Ile Asp Tyr Thr Giu 90 Asp Lys Giu Gly Pro 170 Cys Phe Asp His Asp 250 Leu Val Ile Tyr Ile Glu Val1 Ser 75 Ser Thr Pro Lys Leu 155 Giu Thr Lys Leu Met 235 Ser Thr Ala Val Leu 315 Asp Pro Giu Gly Val1 Lys Leu Lys 140 Asp Pro Ala Pro Phe 220 Arg Thr Phe Al a Ser 300 Asp Ile Asp Leu Asp Val1 Giu Phe 125 Thr Lys Asp Thr Gin 205 Asp Ala Ala Thr Giu 285 Pro Asp Ala Arg Arg Gly Giu Lys 110 Tyr Ile Arg Thr Val1 190 Phe Arg Asp Ile Thr 270 Ser Giu Pro His Ser Tyr Ala Lys Giu Giu Pro His Leu Ser Leu Ala Thr Leu Glu Thr Ile 160 Leu His 175 Arg Pro Pro Val Ile Ala Vai Thr 240 Aia Aia 255 Gly Phe Aia Aia Giu Tyr Val Ala 320 Arg Lys 335 Val Aia Ala Giu Ala 330 WO 01/00843 WO 0100843PCTIIBOO/00923 His Val Lys Val Val Leu Ser Gly Glu Gly Ala Asp Glu Leu Phe Gly 340 345 350 Gly Ile Pro 385 Met Met Val1 Arg Val1 465 Pro Asp Leu Phe Trp 545 Lys Asp Gly Tyr Lys Arg Gly 390 Tyr Pro Tyr Asp Ile 470 Glu Asn Pro Arg Ile 550 Asp Leu Asn Pro 365 Ser Gly Asn Asp Asp 445 Gly Glu Thr Leu Lys 525 Glu Asp Asp Met Glu 605 Phe Lys Ser Phe His 430 Pro Asp Leu Ile Arg 510 Lys Leu Ile Gly Val 590 Asp Glu Val1 Met Glu 415 Arg Val1 Ile Arg Pro 495 Arg Leu Phe Phe Val1 575 Trp, Arg <210> 111 <211> 1284 <212> DNA <213> Corynebacteriun giutamicun WO 01/00843 <220> <221> CDS <222> (101)..(1261) <223> R.XN00116 <400> 111 cgcggcacgc acgctggggg caagcgtcga caagcacaaa PCT/IB00100923 ctttttgctt aattgaatcc tttgcgcacc aatcaatggg ggatcaaata tagtagctgc atg agt aat Met Ser Asn gac ttc Asp Phe gtc Val acc Thr cc t Pro att Ile t tg Leu tac Tyr att Ile gtt Val ggg Gly gat Asp 150 a tg Met aag Lys ccc Pro gcg Ala cgt Arg c ag Gin cgt Arg gtg Val1 gg t Gly ga t Asp gtt Val 140 ttg Leu ccg Pro ttg Leu ttt Phe gg t Gly cgg Arg tat Tyr ga t Asp ttg Leu ttg Leu gcg Ala 125 cc t Pro cat His cat His gcg Ala ggt Gly gca Ala atg Met tcg Ser cat His atc Ile gtg Vali 110 tat Tyr t tg Leu gcg Ala aa t Asn gg t Gly 190 gaa Giu atc Ile tta Leu gcg Ala t tg Leu acg Thr gag Glu gcg Ala cag Gin gcg Ala ccg Pro 175 gtt Val1 gqa Ala cag Gin tcg Ser gat Asp gat Asp act Thr gaa Glu 115 gcg Ala aac Asn aag Lys g tg Val tat Tyr 195 ttg gtg ttg tca gat gag gtg tat gag cat ctt gtt ttt gat gat cag WO 01/00843 Leu Val Leu 200 aag cat gtg Lys His Val 215 acg gtg tcg Thr Val Ser 230 ggg tgg gcg Gly Trp Ala aag cag ttt Lys Gin Phe gcg cat gcg Ala His Ala 280 ggg ctt gag 1027 Gly Leu Glu 295 ggg ctg aag 1075 Gly Leu Lys 310 att ggg gat 1123 Ile Gly Asp gtt ggg gtg 1171 Vai Gly Val aag tgg tcg 1219 Lys Trp Ser 360 ctc cgc gaa 1261 Asp gtc Val gcg Ala gca Ala 250 tct Ser gaa Glu aag Lys cat His gat Asp 330 gcg Ala aag Lys gcg Glu gcg Ala gcg Ala 235 ccg Pro tat Tyr cat His cgg Arg gac Asp 315 ggt Gly att Ile gtt Val gag Tyr 205 ctg Leu acg Thr ccg Pro ggg Gly cag Gin 285 att Ile atg Met gag Glu gtg Val ttt Phe 365 ctc Leu Asp cgc Arg tgg Trp ttg Leu ccg Pro 275 atg Met gat Asp gtt Val att Ile cat His 355 gaa Glu cta Leu PCTIBOO/00923 Asp Gin acg gtg 787 Thr Val aag acg 835 Lys Thr 245 aag gcg 883 Lys Ala 260 gct gtg 931 Ala Val tct aag 979 Ser Lys aag gcg Lys Ala gcg gat Ala Asp 325 gag aag Glu Lys 340 ccg aag Pro Lys gag acg Glu Thr Leu Arg Giu Ala Ala Glu 375 tagtttgaac aggttgttgg ggg 1284 <210> 112 <211> 387 <212> PRT <213> Corynebacterium glutamicum WO 01/00843 PCT/IB00/00923 <400> 112 Met Ser Asn Asp Phe Val Val Ser Arg Leu Arg Pro Phe Gly Glu Thr 1 5 10 Ile Phe Ala Thr Met Thr Gin Arg Ala Val Glu Ala Gly Ala Ile Asn 25 Leu Gly Gin Gly Phe Pro Asp Glu Asp Gly Pro Arg Arg Met Leu Glu 40 Ile Ala Ser Glu Gin Ile Leu Gly Gly Asn Asn Gin Tyr Ser Ala Gly 55 Arg Gly Asp Ala Ser Leu Arg Ala Ala Val Ala Arg Asp His Leu Glu 70 75 Arg Phe Asp Leu Glu Tyr Asn Pro Asp Ser Glu Val Leu Ile Thr Val 90 Gly Ala Thr Glu Ala Ile Thr Ala Thr Val Leu Gly Leu Val Glu Pro 100 105 110 Gly Asp Glu Val Ile Val Leu Glu Pro Tyr Tyr Asp Ala Tyr Ala Ala 115 120 125 Ala Ile Ala Leu Ala Gly Ala Thr Arg Val Ala Val Pro Leu Gin Glu 130 135 140 Val Glu Asn Ser Trp Asp Val Asp Val Asp Lys Leu His Ala Ala Val 145 150 155 160 Thr Lys Lys Thr Arg Met Ile Ile Val Asn Ser Pro His Asn Pro Thr 165 170 175 Gly Ser Val Phe Ser Lys Lys Ala Leu Lys Gin Leu Ala Gly Val Ala 180 185 190 Arg Ala Tyr Asp Leu Leu Val Leu Ser Asp Glu Val Tyr Glu His Leu 195 200 205 Val Phe Asp Asp Gin Lys His Val Ser Val Ala Lys Leu Pro Gly Met 210 215 220 Trp Asp Arg Thr Val Thr Val Ser Ser Ala Ala Lys Thr Phe Asn Val 225 230 235 240 Thr Gly Trp Lys Thr Gly Trp Ala Leu Ala Pro Glu Pro Leu Leu Glu 245 250 255 Ala Val Leu Lys Ala Lys Gin Phe Met Ser Tyr Val Gly Ala Thr Pro 260 265 270 Phe Gin Pro Ala Val Ala His Ala Ile Glu His Glu Gin Lys Trp Val 275 280 285 Ser Lys Met Ser Lys Gly Leu Glu Leu Lys Arg Asp Ile Leu Arg Thr 290 295 300 Ala Leu Asp Lys Ala Gly Leu Lys Thr His Asp Ser Met Gly Thr Tyr 305 310 315 320 WO 01/00843 WO 0100843PCTTIBOOIOO923 Phe Ile Vai Ala Asp Ile Gly Asp Arg Asp Gly Ala Giu Phe Cys Phe 325 330 335 Giu Leu Ilie Glu Lys Val Gly Val Ala Ala Ile Pro Val Gin Ala Phe 340 345 350 Val Asp His Pro Lys Lys Trp, Ser Ser Lys Val Arg Phe Ala Phe Cys 355 360 365 Lys Lys Glu Giu Thr Leu Arg Glu Ala Ala Glu Arg Leu Lys Gly Ile 370 375 380 Lys Lys Leu 385 <210> 113 <211> 607 <212> DN.A <213> Corynebacteriun glutainicum <220> <221> CDS <222> (101)..(607) <223> FRXA00116 <400> 113 tttgcgcacc aatcaatggg ggatcaaata tagtagctgc atgagtaatg acttcgtcgt ttctaggctt agaccctttg gtgaaacgat ttttgcaacc atg acc cag cga gct 11: Met Thr Gin Arg Ala gtt Val gg t Gly aat Asn gtg Val1 tcg Ser gtg Val tat Tyr gag Giu cc t Pro aat Asn gc t Ala gag Glu t tg Leu tac Tyr gcg Al a cgt Arg cag Gin cgt Arg gtg Val1 ggt Giy gat Asp ggt Gly cgg Arg tat Tyr gat Asp t tg Leu t tg Leu gcg Al a 105 gca Ala atg Met tcg Ser cat His atc Ilie gtg Val tat Tyr cag Gin tcg Ser gat Asp ga t Asp act Thr gaa Giu gcg Ala 1 ttt Phe cag Gin tcg Ser gag Glu gcg* Ala atc Ile gcg Ala cct Pro att Ile t tg Leu tac Tyr att Ile gtt Val ggg Gly gat

ASP

c tc Leu agg Arg aac Asn acg Thr t tg Leu gcg Ala 115 WO 01/00843 WO 0100843PCTJIBOO/00923 g tg Val ga t Asp aat Asn 150 gcg gtt Ala Val 120 aag ttg Lys Leu 135 tcg ccg Ser Pro cct ttg cag gag gtg gag aac tcg tgg gat gtg gat gtc Pro Leu Gin Giu Val Glu Asfl Ser Trp Asp Val Asp Val 125 130 cat gcg gcg gtg act aag aag acg cgg atg att atc gtt His Ala Ala Val Thr Lys Lys Thr Arg Met Ile Ile Val 140 145 cat aat ccg acg ggt tcg gtg ttt tct aag aag gcg ttg His Asn Pro Thr Gly Ser Val Phe Ser Lys Lys Ala Leu 155 160 165 499 547 595 aag cag ttg gcg Lys Gin Leu Ala <210> 114 <211> 169 <212> PRT <213> Corynebacterium glutamicum <400> 114 Met Thr Gin Arg Aia Val Giu Ala Gly 1 Phe Gin Ser Giu Ala Ile Al a Trp Arg 145 Ser 5 Asp Gly Ala Asp Thr Pro Arg Val1 Val Leu 165 Gly Asn Val Ser 70 Val Tyr Val1 Asp Asn 150 Lys Pro Asn Ala Giu Leu Tyr Ala Lys 135 Ser Gin Arg Gin Arg Val Gly Asp Val 120 Leu Pro Leu Ala 10 Met Ser His Ile Val 90 Tyr Leu Ala Asn Ile Leu Ala Leu Thr 75 Glu Ala Gin Ala Pro 155 Leu Ile Arg Arg Gly Gly Ala Val1 125 Thr Gly Gly Ala Gly Phe Ala Asp Ile 110 Giu Lys Ser Gin Ser Asp Asp Thr Glu Ala Asn Lys Val Gly Glu Ala Leu Glu Val1 Leu Ser Thr Phe 160 <210> 115 <211> 1230 <212> DNA WO 01/00843 WO 0100843PCTIiBOO/00923 <213> Corynebacterium glutaiicun <220> <221> CDS <222> (1207) <223> RXN00618 <400> 115 gctgcattag agggtcgtat ctcgatctaa aagcagtacg gaagccaagc actagaagca atgttcagcc gtttcgcgtc cagataggct tgtctcttat atg cag atg ttg gac ii! Met Gin Met Leu Asp 1 cga gtc cac cgt Arg Val His Arg cgc Arg agg cgc gaa ggc Arg Arg Glu Gly gac acc tta atg Asp Thr Leu Met ttc tgc Phe Cys gct ggc cag Ala Gly Gin gag atc gct Glu Ile Ala tca act ggt gcg cca gaa gca gtc atc Ser Thr Gly Ala Pro Giu Ala Val Ile gaa gaa gca Giu Giu Ala gtg att ggt Val Ile Gly ctt cgc tcg ggt Leu Arg Ser Gly ttg gga tac acc Leu Gly Tyr Thr gag Glu gat cgt Asp Arg gag ttc cgt gaa Glu Phe Arg Giu atc gcc gat tgg Ile Ala Asp Trp tct gct act tat Ser Ala Thr Tyr gac gta gac acc aac Asp Val Asp Thr Asn ggt gga ttc gtg gca Gly Gly Phe Val Ala gac aat gtt att Asp Asn Val Ile acc acc ggt tct Thr Thr Gly Ser tcg ttt atc gcc acc Ser Phe Ilie Ala Thr 95 ttg gat cac ggg Leu Asp His Gly gat tat Asp Tyr 100 gtg gca atg Val Ala Met tct ttg ggg Ser Leu Gly 120 acc ccg ggg tac Thr Pro Giy Tyr gca tat cgc aat att ctg gaa Ala Tyr Arg Asn Ile Leu Giu 115 gcg aag gtt ctg Ala Lys Val Leu ctg cgc tgt act Leu Arg Cys Thr gca Ala 130 gag act cgt Glu Thr Arg ttc cag Phe Gin 135 cca acc gct caa atg ttg gag gaa ctg Pro Thr Ala Gin Met ILeu Glu Giu Leu 140 cac aag ccg aag His Lys Pro Lys gtt att gtc acc agc cca gga aac cca Val Ilie Val Thr Ser Pro Gly Asn Pro 155 ggc acc atc att Gly Thr Ile Ile ccg gaa gag cta Pro Glu Glu Leu gag Glu 170 cgc atc gcc aag Arg Ilie Ala Lys tgc gat gac aat Cys Asp Asp Asn gat gct Asp Ala 180 gtt ctt atc Val Leu Ile tct Ser 185 gat gag gac tac Asp Glu Asp Tyr ggc atg agc ttt Gly Met Ser Phe ggt cgt ccg dly Arg Pro 195 WO 01/00843 WO 0100843PCTfIiBOO/00923 ctg gca act gcg cat cag ttt tcc aag aac gcc atc gtg gtg ggt acc 739 Leu Ala Thr Ala His Gin Phe Ser Lys Asn Ala Ile Val Val Gly Thr 200 205 210 ttg tcc aag tac ttc tcc atg acg ggt tgg cgc gtg ggt tgg atc atc 787 Leu Ser Lys Tyr Phe Ser Met Thr Gly Trp, Arg Val Gly Trp Ile Ile 215 220 225 gtt cca gat gag ctg gtc aca ccg att gaa aac ctg cag gct tct ctt 835 Val Pro Asp Glu Leu Val Thr Pro Ile Glu Asn Leu Gin Ala Ser Leu 230 235 240 245 tcc ttg tgt gct cct gcc atc ggg cag gct gcg gga cgc gca gcc ttc 883 Ser Leu Cys Ala Pro Ala Ilie Gly Gin Ala Ala Gly Arg Ala Ala Phe 250 255 260 act ttg gag gct ggg gcc gaa ctt gat gcc cac gtt gaa gcg tat cgc 931 Thr Leu Glu Ala Gly Ala Giu Leu Asp Ala His Val Glu Ala Tyr Arg 265 270 275 gag gcc cgg gag gtg ttc gtc gat aag ctc cct gaa atc ggg ctt ggc 979 Giu Ala Arg Glu Val Phe Val Asp Lys Leu Pro Glu Ile Gly Leu Giy 280 285 290 act ttc gcc gac ccg gat ggc ggc ctg tat ttg tgg gtc gat gtt tct 1027 Thr Phe Ala Asp Pro Asp Gly Gly Leu Tyr Leu Trp Val Asp Val Ser 295 300 305 gca tac acc gat gat tca gag gaa tgg gca ttg cgt ttg ctc gat gaa 1075 Ala Tyr Thr Asp Asp Ser Giu Glu Trp Ala Leu Arg Leu Leu Asp Giu 310 315 320 325 gcg ggc gtg gcc gtc gcg ccg ggt gtt gat ttt gat cct gag gaa ggc 1123 Ala Gly Vai Ala Val Ala Pro Gly Val Asp Phe Asp Pro Glu Giu Giy 330 335 340 cac aag tgg att cgt ttg agc ctg tgc gcg tca aag gaa gac acc att 1171 His Lys Trp Ile Arg Leu Ser Leu Cys Ala Ser Lys Giu Asp Thr Ile 345 350 355 gaa ggt gtg cgc aaa atc gga gaa ttc atc aaa aaa tagcagcgac 1217 Glu Gly Vai Arg Lys Ile Gly Glu Phe Ile Lys Lys 360 365 taggttagtt tcg 1230 <210> 116 <211> 369 <212> PRT <213> Corynebacterium giutaniicum <400> 116 Met Gin Met Leu Asp Arg Val His Arg Arg Arg Arg Glu Gly Lys Asp 181 WO 01/00843 WO 0100843PCTIBOOIOO923 1 Thr Val Thr His Thr Asp Arg Thr Pro :145 Gly Asp Ser Ile Val1 225 Leu Gly Val1 Giu Trp 305 Arg Met Glu Val Ala Gly Gly Ile 115 Glu Lys Ile Asn Gly 195 Val1 Trp Ala Al a Ala 275 Gly Asp Leu Phe Glu Ile Thr Ser Asp 100 Leu Thr Pro Ile Asp 180 Arg Gly Ile Ser Ala 260 Tyr Leu Val Asp 5 Cys Ala Gly Tyr Ser Tyr Glu Arg Lys Asp 165 Ala Pro Thr Ile Leu 245 Phe Arg Gly Ser Giu 325 Ala Giu Asp Asp 70 Gly Val1 Ser Phe Ala 150 Pro Val1 Leu Leu Val1 230 Ser Thr Glu Thr Ala 310 Ala Gly Ile Arg Val Gly Ala Leu Gin 135 Val1 Giu Leu Ala Ser 215 Pro Leu Leu Ala Phe 295 Tyr Gly Gin Ala 40 Glu Asp Phe Met Gly 120 Pro Ile Glu Ile Thr 200 Lys Asp Cys Glu Arg 280 Ala Thr Val1 Thr Ser Giu Pro 75 Ser Pro Val1 Gin Ser 155 Arg Giu Gin Ser Val1 235 Al a Ala Phe Asp Ser 315 Ala Gly Gly Arg Asp Phe Gly Leu Met 140 Pro Ile Asp Phe Met 220 Thr Ile Glu Val1 Gly 300 Giu Pro Al a Pro Ile Asn Ile Tyr Asn 125 Leu Gly Ala Tyr Ser 205 Thr Pro Gly Leu Asp 285 Gly Glu Gly Pro Leu Ala Val Ala Pro 110 Leu Glu Asn Lys His 190 Lys Gly Ile Gin Asp 270 Lys Leu Trp Val1 Giu Gly Asp Ile Thr Ala Arg Glu Pro Trp 175 Gly Asn Trp Giu Aia 255 Ala Leu Tyr Ala Asp 335 Ala Tyr Trp Val1 Leu Tyr Cys Leu Thr 160 Cys Met Ala Arg Asn 240 Al a His Pro Leu Leu 320 Phe WO 01/00843 WO 0100843PCTf1B00100923 Asp Pro Giu Glu Gly His Lys Trp Ile Arg Leu Ser Leu Cys Ala Ser 340 345 350 Lys Glu Asp Thr Ile Giu Gly Val Arg Lys Ile Gly Giu Phe Ile Lys 355 360 365 Lys <210> 117 <211> 657 <212> DNA <213> Corynebacteriun glutarnicum <220> <221> CDS <222> (101)..(634) <223> FRXA00618 <400> 117 cccaacgggc accatcattg atccggaaga gctagagcgc atcgccaagt ggtgcgatga caatgatgct gttcttatct ctgatgagga ctaccacggc atg agc ttt Met Ser Phe ccg Pro acc Thr atc Ilie ctt Leu t tc Phe cgc Arg ggc Gly tc t Ser cag Gin tcc Ser gtc Val gcc Aia gcc Ala t tc Phe ga t Asp tca Ser ttt Phe atg Met aca Thr atc Ile gaa Giu gtc Val ggC Gly gag Glu 125 ggt cgt Gly Arg gtg ggt Val Giy tgg atc Trp Ile gct tct Ala Ser gca gcc Ala Ala gcg tat Ala Tyr ggg ctt Gly Leu 100 gat gt Asp Val ctc gat Leu Asp gaa gcg ggc gtg gcc gtc gcg ccg ggt gtt gat ttt gat cct gag gaa WO 01/00843 Glu Ala Gly Val Ala Val Ala Pro Gly 135 140 ggc cac aag tgg att cgt ttg agc ctg Gly His Lys Trp Ile Arg Leu Ser Leu 150 155 att gaa ggt gtg cgc aaa atc gga gaa Ile Glu Gly Val Arg Lys Ile Gly Giu 170 taggttagtt tcg <210> 118 <211> 178 <212> PRT <213> Corynebacterium glutamicum <400> 118 Met Ser Phe Gly Arg Pro Leu Ala Thr PCTIBOOIOO923 veil Asp Phe Asp Pro Giu Glu 145 tgc gcg tca aag gaa gac acc 595 Cys Ala Ser Lys Glu Asp Thr 160 165 ttc atc aaa aaa tagcagcgac 644 Phe Ile Lys Lys 175 1 Ala Arg Asn Ala His Pro Leu Leu Phe 145 Ser Lys Ile Val1 Leu Gly Val Glu Trp Arg 130 Asp Lys Lys Val Gly Gin Arg Giu Ile Val1 115 Leu Pro Giu Val Trp Ala Ala Ala G ly 100 Asp Leu Glu Asp 5 Gly Ile Ser Ala Tyr Leu Val1 Asp Glu Thr 165 Thr Ile Leu Phe Arg Gly Ser Glu Gly 150 Ile Lys Asp Cys Gi u Arg Ala 105 Thr Val1 Trp, Val1 Ala Tyr Glu Ala Ala Giu Asp Asp Ala Ile Arg 170 Gin Ser Val1 Ala Ala Phe Asp Ser Ala 140 Leu Ile Phe Met Thr Ile Glu Val1 Gly Glu 125 Pro Ser Gly <210> 119 <211> 385 <212> DNA <213> Corynebacteriun giutamicum WO 01/00843 WO 0100843PCT/IBOO/00923 <220> <221> CDS <222> (101)..(385) <223> FR.XA00627 <400> 119 gctgcattag agggtcgtat ctcgatctaa aagcagtacg gaagccaagc actagaagca atgttcagcc gtttcgcgtc cga Arg gc t Ala gag Giu gat Asp gac Asp gg t Gly c ac His cag Gin gc t Ala gag Giu gac Asp t tc Phe cg t Arg ccg Pro ctt Leu t tc Phe acc Thr g tg Val cagataggct tgtctcttat atg cag atg ttg gac Met Gin Met Leu Asp 1 acc tta atg ttc tgc Thr Leu Met Phe Cys gtc atc gaa gaa gca Val Ile Giu Giu Ala acc gag gtg att ggt Thr Giu Val Ile Gly cac tct gct act tat His Ser Ala Thr Tyr acc acc ggt tct tca Thr Thr Gly Ser Ser <210> 120 <211> <212> PRT <213> Corynebacteriun giutanicun <400> 120 Met Gin Met Leu Asp Arg Vai HisI 1 Thr Leu Met Phe Cys Ala Gly Gin 1 Val Ile Giu Giu Ala Giu Ile Ala 1 40 Thr Giu Val Ile Gly Asp Arg Giu 1 His Ser Ala Thr Tyr Asp Val Asp Thr Thr Giy Ser Ser Gly Giy Phe '1 Giu Ala Pro Ile Asn Ile Gly Pro Leu Ala Val1 Ala WO 01/00843 WO 0100843PCT/IBOO/00923 <210> <211> <212> <213> <220> 121 1434

DNA

Corynebac teriun glut aric ur <221> CDS <222> (101)..(1411) <223> RXA02550 <400> 121 gatcttaggc agccgtggga ttacaccctt ttagagctag aacagtaaaa attcacccaa tagctttcaa ctacgcacac aaagtggcaa cattgagcgg gtg act aca gac Val Thr Thr Asp cgc aaa acc tct Arg Lys Thr Ser acc acc gac acc gcc aac aag gct gtg Thr Thr Asp Thr Ala Asn Lys Ala Val ggc gcg Gly Ala gat cag gca Asp Gin Ala cag tcg gag Gin Ser Giu cgt ccc act cgg Arg Pro Thr Arg aca act cgc cgc Thr Thr Arg Arg atc ttc gat Ilie Phe Asp ggc ccg gtg Gly Pro Val aag atg aag gac Lys Met Lys Asp ctg tac gag atc Leu Tyr Giu Ilie gcc gcg Ala Ala gag gcg gaa cgc Giu Ala Glu Arg gag ctt gat ggg Giu Leu Asp Gly aac atc tta aag Asn Ile Leu Lys aac acg gga aat Asn Thr Gly Asn gcc gtg ttc gga Ala Vai.Phe Gly gat gcc ccc gac Asp Ala Pro Asp att atg cgt gac Ile Met Arg Asp atc gcc aac ctt Ile Ala Asn Leu cca Pro act tcc caa ggg Thr Ser Gin Gly tat tcc Tyr Ser 100 acc tcc aaa Thr Ser Lys gaa gtt gtg Giu Val Val 120 ggc Gly 105 att att ccg gcc Ile Ile Pro Ala cga gca gtg gtc Arg Ala Val Val acc cgc tac Thr Arg Tyr 115 gtg ttc tta Val Phe Leu ccc gga ttc ccc Pro Gly Phe Pro ttc gat gtt gat Phe Asp Val Asp gat Asp 130 ggc aac Gly Asn 135 ggt gtc tca gaa Gly'Val Ser Giu atc acc atg acc Ile Thr Met Thr acc Thr 145 caa gca ctc ctc Gin Ala Leu Leu gac ggc gat gaa Asp Gly Asp Giu ctt atc ccc gca Leu Ile Pro Ala gac tac cca ctg Asp Tyr Pro Leu act gcc gca acc tcc ctg gct ggt ggt aag cct gtg cac tac ctc tgt Thr Ala Ala Thr Ser Leu Ala Gly Gly Lys Pro Val His Tyr Leu Cys WO 01/00843 gat gag Asp Glu atc tca Ile Ser acg gga Thr Gly 215 gca cgc Ala Arg 230 att ctc Ile Leu gat ctc Asp Leu gca gga Ala Gly gca cgt 1027 Ala Arg 295 tgc cca 1075 Cys Pro 310 cgc cag 1123 Arg Gin cag cgc 1171 Gin Arg tgt gtg 1219 Cys Val aac gtg 1267 Asn Val 375 170 gac Asp ac c Thr tac Tyr gac Asp gat Asp 250 atc Ile gc t Ala att Ile cca Pro tac Tyr 330 gca Ala a tg Met atc Ile aac Asn gc t Ala cgc Arg 220 ctg Leu gag Giu tac Tyr tgg Trp ggc Gly 300 cag Gin ctc Leu acg Thr gc t Ala gac Asp 380 cca Pro att Ile 205 cgg Arg att Ile cac His aac Asn atg Met 285 C tC Leu ca c His act Thr aaa Lys cta Leu 365 gac Asp aag Lys 195 aac Asn gtc Vai tac Tyr ctt Leu tac Tyr 275 aag Lys act Thr c tg Leu C tc Leu gg t Gly 355 ctc Leu ga t Asp PCT/IBOO/00923 180 tcc aaa 691 Ser Lys aac ccc 739 Asn Pro gag att 787 Giu Ile gac cgc 835 Asp Arg 245 gca cca 883 Ala Pro 260 cgc gtc 931 Arg Vai caa tac 979 Gin Tyr cga ctc Arg Leu ggt gga Gly Giy 325 ctg gaa Leu Giu 340 gtc agc Val Ser gac ccc Asp Pro ctt ctc Leu Leu WO 01/00843 WO 0100843PCTI/iBOO/00923 cgt gcc gag aaa atc ctc atg gtt cag 1315 Arg Ala Glu Lys Ile Leu Met Val Gin 390 395 cat cac gat cac ttc cga gtg gtc acc 1363 His His Asp His Phe Arg Val Val Thr 410 gaa aac gca att gag cgc ctg ggt aac 1411 Giu Asn Ala Ile Giu Arg Leu Gly Asn 425 430 tagtagttgt taggattcac cac 1434 <210> 122 <211> 437 <212> PRT <213> Corynebacterium giutamicun <400> 122 Val Thr Thr Asp Lys Arg Lys Thr Ser ggc act ggc ttc Gly Thr Gly Phe 400 ctg cca tgg gca Leu Pro Trp Ala 415 ttc ctg tcc act Phe Leu Ser Thr 1 Lys Arg Ile His Asp Ser Val1 Asp Thr 145 Asp Val1 Ala Arg Arg Asn Ala Gin Val Asp 130 Gin Tyr His Gin Ala Ser Giu Ala Giu 55 Asn Thr Met Arg Ser Lys Val Vai 120 Asn Gly 135 Asp Gly Ala Ala Giu Glu Ala Lys Al a Gly Asp Gly 105 Pro Val1 Asp Thr Asp 185 Lys Arg Met Giu Asn Met Ile Gly Ser Giu Ser 170 Asp Thr Pro Lys Arg Pro 75 Ile Ile Phe Giu Val 155 Leu Trp Thr Thr Asp Met Ala Ala Pro Pro Leu 140 Leu Ala Asn cca Pro 405 t tg Leu cag Gin Asn Thr Giu Gly Phe Thr Ala Val1 Thr Pro 160 Pro Glu WO 01/00843 WO 0100843PCT/TB00100923 Asp Ilie Lys Ser Lys Ile Ser Giu Lys Thr Lys Ala Ile Val Val Ile 195 200 205 Asn Pro Asn Asn Pro Thr Gly Ala Val Tyr Pro Arg Arg Val Leu Giu 210 215 220 Gin Ilie Val Glu Ile Ala Arg Giu His Asp Leu Leu Ile Leu Ala Asp 225 230 235 240 Giu Ilie Tyr Asp Arg Ile Leu Tyr Asp Asp Ala Giu His Ile Ser Leu 245 250 255 Ala Thr Leu Ala Pro Asp Leu Leu Cys Ile Thr Tyr Asn Gly Leu Ser 260 265 270 Lys Ala Tyr Arg Val Ala Gly Tyr Arg Ala Gly Trp Met Val Leu Thr 275 280 285 Gly Pro Lys Gin Tyr Ala Arg Gly Phe le.Glu Gly Leu Giu Leu Leu 290 295 300 Ala Giy Thr Arg Leu Cys Pro Asn Val Pro Ala Gin His Ala Ile Gin 305 310 315 320 Val Ala Leu Gly Gly Arg Gin Ser Ile Tyr Asp Leu Thr Gly Giu His 325 330 335 Gly Arg Leu Leu Giu Gin Arg Asn Met Ala Trp Thr Lys Leu Asn Giu 340 345 350 Ile Pro Gly Vai Ser Cys Val Lys Pro Met Gly Ala Leu Tyr Ala Phe 355 360 365 Pro Lys Leu Asp Pro Asn Val Tyr Giu Ile His Asp Asp Thr Gin Leu 370 375 380 Met Leu Asp Leu Leu Arg Ala Giu Lys Ilie Leu Met Vai Gin Giy Thr 385 390 395 400 Gly Phe Asn Trp Pro His His Asp His Phe Arg Val Vai Thr Leu Pro 405 410 415 Trp, Ala Ser Gin Leu Giu Asn Ala Ile Giu Arg Leu Gly Asn Phe Leu 420 425 430 Ser Thr Tyr Lys Gin 435 123 <211> 1701 <212> DNA <213> Corynebacterium giutamicum <22 0> <221> CDS <222> (101)..(1678) <223> RXA02193 <400> 123 cccccgaaaa tgtagtgggt agttccacac tgcctcttac aagtacgtag gataatccac 189 WO 01/00843 agcaccattg tgatttcctt caacttgtga gaggcagtac atg tct aag Met Ser Lys PCTIIBOO/00923 aac Asn g tg Val1 agc Ser aaa Lys t cc Ser caa Gin a tg Met aca Thr atc Ile cag Gin 150 gcc Aia atc Ile tcc Ser c tc Leu gca Ala aac Asn tcg Ser atc Ile tac Tyr acc Thr aaa Lys caa Gin gaa Giu ggt Giy ctt Leu 170 atg Met ggt Gly att Ile gac Asp caa Gin gaa Giu gaa Giu ggg Gly acc Thr aag Lys aaa Lys cgc Arg acc Thr 155 gag Giu gat Asp ttc Phe gat Asp tca Ser atc Ile cgt Arg tcg Ser gtg Val1 atc Ile gcc Ala gca Aia tgt Cys 140 tca Ser t tc Phe ga t Asp cgc Arg gag Piu 220 aaa Lys tcg Ser aaa Lys gaa Giu gcg Ala ga t Asp aac Asn tgg Trp ccc Pro 145 acc Thr aag Lys cag Gin gc t Ala t tc Phe 225 gcc Ala cag Gin acc Thr ctt Leu gtg Va 1 ttc Phe cgc Arg gc t Ala 130 atc Ile aac Asn 9gc Gly t cc Ser gga Gly 210 cgc Arg gaa Giu tct Ser acg Thr cag Gin gac Asp att Ile cga Arg 115 tgt Cys gat Asp gag Giu gag Glu acc Thr 195 ctg Leu cac His acg Thr gac Asp tca Ser gtt Val1 atc Ile aac Asn cgc Arg 100 c tg Leu ga t Asp gtg Val1 gtt Val tac Tyr 180 aac Asn cag Gin aag Lys WO 01/00843 WO 0100843PCTIBOO/00923 aat Asn 230 gc t Ala gag ttt gtc gac Giu Phe Val Asp a tc Ile 235 t tg Leu atc aag atg ggc Ile Lys Met Gly acc cag ttg cag Thr Gin Leu Gin ga t Asp 245 gtt ccc atg Val Pro Met ggc gaa gag Gly Giu Giu gca ttc gcg Ala Phe Ala cac aac His Asn 260 ctc ctc Leu Leu ctc gca gaa Leu Ala Giu gag gtc aat Glu Vai Asn 2 80 acc gtg ctg Thr Val Leu gct gcc aac Ala Ala Asn ggt gca acc Gly Ala Thr gca ggc 1027 Ala Gly 295 ctg gaa 1075 Leu Glu 310 ggt gca 1123 Gly Ala ctg tcc 1171 Leu Ser gct ggc 1219 Ala Gly atc atg 1267 Ile Met 375 gtc tgc 1315 Val Cys 390 gaa gct 1363 Giu Ala tcc ctc 1411 Ser Leu tac cgc cac cag gtt Tyr Arg His Gin Vai 300 cta aag tcc gca cgt Leu Lys Ser Ala Arg 315 tat gtt cat gcg cac Tyr Val His Ala His 330 aag atc tgt aac gat Lys Ile Cys Asn Asp gca Ala 285 gtc Val1 ga t Asp ggt act ggt Gly Thr Gly gtg Val 290 gct gct ctg tct gag Ala Ala Leu Ser Giu 305 ctc atc gag gct acc Leu Ile Giu Ala Thr aac act cca Asn Thr Pro gtc acc gga Val Thr Gly tct gac acc Ser Asp Thr 325 979 320 tcc gca atc aag Ser Ala Ile Lys cgt gca gcc atg aaa Arg Ala Ala Met Lys 340 335 cta cgt Leu Arg 345 ttg aac Leu Asn 350 gaa atc aac ctg cca Giu Ile Asn Leu Pro 365 aag gtc aac cca gtg Lys Val Asn Pro Val ctg ctg tct tct ggt cct cgt Leu Leu Ser Ser Gly Pro Arg 355 cca cgc cag gct ggt tcc tcc Pro Arg Gin Ala Giy Ser Ser 370 atc cca gaa gtg gtc aac cag Ile Pro Giu Val Val Asn Gin gcc Al a 380 385 ttc aag gtc ttc Phe Lys Val Phe 395 ggc cag ttg cag Gly Gin Leu Gin 410 ttc cag tca ctg Phe Gin Ser Leu 425 ggt aac gat ctc acc Gly Asn Asp Leu Thr 400 ctc aac gtc atg gag Leu Asn Vai Met Glu 415 cgc atc ctg ggc aat Arg Ile Leu Gly Asn 430 gtc Val acc atg gct gcg Thr Met Ala Ala 405 cca gtc att ggc gaa Pro Val Ile Gly Glu 420 gca gcc aag act ttg Ala Ala Lys Thr Leu 435 WO 01/00843 WO 0100843PCT/IBOO/00923 cgt gag aag tgc gtc gta gga atc 1459 Arg Giu Lys Cys Val Val Gly Ile 440 445 gct tac gtt gat aac tcc atc ggg 1507 Ala Tyr Val Asp Asn Ser Ile Giy 455 460 ctg ggc cac gac att gga gat cag 1555 Leu Gly His Asp Ile Gly Asp Gin 470 475 ggt cga cca gtg cgt gaa ctc atc 1603 Gly Arg Pro Val Arg Glu Leu Ile 490 aag acg ctc gag gca gtc ctg tcc 1651 Lys Thr Leu Glu Ala Val Leu Ser 505 ttc cgc gga agg ctc tac ttg gag 1698 Phe Arg Gly Arg Leu Tyr Leu Glu 520 525 tac 1701 <210> 124 <211> 526 <212> PRT <213> Corynebacteriun glutamicun <400> 124 Met Ser Lys Thr Ser Asn Lys Ser 1 5 Lys Ala Giu Asp Ile Val Asn Gly Ser Gin Ser Ser Asp Ser Ala Ala Lys Thr Thr Val Gin Lys Lys Phe Giu Leu Gin Ilie Pro Ser His Ala Ala Vai Asp Asn Phe Gin Ile Ser Asp Phe Ile Arg Gly Met Vai Gin 100 acc gcc aac gct gat gtt Egc Thr Ala Asn Ala Asp Vai Cys 450 att atc act tac ctg aac cca Ile Ile Thr Tyr Leu Asn Pro 465 atc ggt aag gaa gca gcc gaa Ile Giy Lys Glu Ala Ala Giu 480 ctg gaa aag aag ctc atg gat Leu Giu Lys Lys Leu Met Asp 495 500 aag gag aac ctc atg cac cca Lys Giu Asn Leu Met His Pro 510 515 aac taatccaaga tctcgtctga Asn Ser Glu Val Arg Tyr Arg Val 105 Ala Asn Ser Ile Tyr Thr Lys Lys Ala Ala WO 01/00843 PCT/IBOO/00923 Leu His Thr Leu Pro Ala Gin Lys Ala Giu Ala Ile Val Asn Trp' Pro 145 Thr Lys Gin Ala Phe 225 Thr Ala Ala Gly Ser 305 Ala Arg Ser Gin Glu 385 Val1 Pro Ala Arg Ala 130 Ile Asn Gly Ser Giy 210 Arg Gin Phe Asn Val1 290 Giu Thr Ala Ser Ala 370 Val1 Thr Val1 Ala 120 Gin Phe Val1 165 His Asp Thr Gly Asp 245 Asn Leu Pro Gly Thr 325 Lys Arg Ser Gin Ala 405 Giu Leu Ile Gin 150 Ala Ile Ser Leu Asn 230 Ala Leu Giu Ala Leu 310 Gly Leu Ala Ile Val1 390 Giu Ser Axg 125 Met Leu Leu Val Leu 205 Leu Lys Giu Leu Ala 285 Val1 Asp Ser Leu Leu 365 Pro Asn Asn Ile Ile

ASP

Asn Giy Asn 190 Giy Gin Met Giu Arg 270 Ile Ala Leu Aia Arg 350 Pro Val1 Asp Val1 Leu 430 Thr Gin Met His 175 Met Ile Val1 Giy Phe 255 Giu G ly Ala Ile Ile 335 Leu Pro Ile Leu Met 415 Gly Ala WO 01/00843 WO 0100843PCT/IBOO/00923 Ala Tyr 465 Giu Lys Leu Cys Arg Ala Tyr 455 Pro Phe Leu Gly 470 Glu Thr Gly Arg 485 Asp Giu Lys Thr 500 Pro Met Phe Arg 440 Val1 His Pro Leu G ly 520 Asp Asn Ser Ile 460 Asp Ile Gly Asp 475 Val Arg Glu Leu 490 Giu Ala Val Leu 505 Arg Leu Tyr Leu Ile Ile Leu Lys 510 Asn Ile Gly Glu 495 Glu <210> 125 <211> 1098 <212> DNA <213> Corynebacteriun giutanicurn <220> <221> CDS <222> (101)..(1075) <223> RXA02432 <400> 125 cacgtgattc atttgtgacc aacaaccgaa actgagccag aagactgtca atcccctgct gtgcacataa caactgcagc tagttgatac gctagagcgc atg tcg aag cag cac Met Ser Lys Gin His tcC Ser gtt Val aat Asn a tc Ile atc Ile atc Ile gta Val1 t ta Leu atc Ile c tg Leu cgg Arg ctt Leu acg Thr acc Thr aac Asn acc Thr ctt Leu t tc Phe ga t Asp gtt Val cac His aa t Asn acg Thr ccc Pro aa t Asn tcc Ser cat His ggc Gly gat Asp ggc Giy ac c Thr gga Gly tcc Ser aag Lys ac c Thr gaa Giu gga G ly gtc Val gcg Ala t cc Ser g tg Val gat Asp 1 tc Ser tgt Cys gac Asp t tc Phe gag Giu ccg Pro gag Glu c t Pro tcc Ser ctt Leu atc Ile c tc Leu gtt Val1 gcc Al a caa Gin gac Asp gcg Ala cac His ga t Asp gtt Val1 100 atc Ile WO 01/00843 WO 0100843PCT/IBOOI00923 gta gac Val Asp caa aaa Gin Lys 135 gaa gcc Giu Ala 150 ctc att Leu Ilie tgg cac Trp His cca gag Pro Glu gtc gaa Val Glu 215 gct gcc Aia Ala 230 tca ggc Ser Gly gac gct Asp Ala gta tcc Val Ser aag ggc 1027 Lys Gly 295 ttg ctc 1075 acc Thr 120 ccc Pro tgc Cys gtc Val acc Thr cgc Arg 200 atc Ile atc Ile aa t Asn gga Gly gga Gly 280 gc t Al a gcg ttc Phe ttc Phe c tc Leu t tc Phe tc t Ser 185 ccc Pro atc Ile gct Ala gt t Vai att Ile 265 gtg Val1 gtg Val1 att ctt Leu gat Asp atc Ile ggt Giy 170 ga t Asp gat Asp ccc Pro gcc Ala ggt Gly 250 ccc Pro tat Tyr gga Gly gcc gat gat Asp Asp cat ccc His Pro 140 gca tcc Ala Ser 155 cac gcc His Ala gag ctg Giu Leu gcg ctg Ala Leu gca tac Ala Tyr 220 ggt gct Giy Ala 235 tcc cgc Ser Arg gtg gtg Val Val ggc ggt Gly Gly tct cgc Ser Arg 300 att gcg ccc cgc cca gtc att ttc Ile cca Pro 145 cgc Arg cgc Arg aac Asn t tg Leu ggc Gly 225 gtg Val cta Leu gtt Val1 gcg Ala ggt Gly 305 ccg Pro Phe 130 aac Asn gga Gly ggc Gly ggc Gly gcg Ala 210 gca Ala gaa Glu gg t Gly cct Pro act Thr 290 cag Gin gtg Val acc Thr aac Asn att Ile tgc Cys cct Pro 195 ga t Asp atg Met gca Ala aaa Lys cgt Arg 275 ttg Leu gca Ala acg Thr gcc Al a ttc Phe gca Ala 165 aaa Lys gaa Glu tc t Ser gaa Glu gga Gly 245 ctt Leu gaa Giu gcg Ala att Ile tac T'lyr 325 Leu Leu Ala Ile Ala Ile Ala 310 315 taatttcgcc cttggtcttg cat 1098 <210> 126 WO 01/00843 <211> 325 <212> PRT <213> Corynebacteriui glutamicun <400> 126 Met Ser Lys Gin His Ser Thr Pro Leu PCT.LBOO/00923 1 Ser Cys Asp Phe Giu Pro Glu Ile Pro 145 Arg Arg Asn Leu Gly 225 Val1 Leu Val1 Ala Gin Asp Ala His Asp Val 100 Ile Gly Leu Gly Val1 180 Glu Val1 Val1 Met Ala 260 Gly Ala 5 Lys Al a Pro Giu Ser G'ly Ala Al a Phe Ala 165 Lys Giu Ser Glu Gly 245 Leu Glu Ala Val His Pro Arg Ala Val Thr 120 Pro Cys Val1 Thr Arg 200 Ile Ile Asn Gly Gly 280 Ala Asn Thr Leu Phe Asp Val1 His Leu Asp Ile Gly 170 Asp Asp Pro Ala Gly 250 Pro Tyr Gly Asp Gly Thr Gly Ser Lys Thr Asp Pro 140 Ser Ala Leu Leu Tyr 220 Ala Arg Val1 Gly Arg 300 Giu Gly Val Ala Ser Val Asp Pro 125 Giu Asp Val Ala Pro 205 Pro Gin Met Met Ala 285 Tyr Giu Thr Ser Gin Met Leu Ser 110 Arg Ala Pro Ile Phe 190 Val Gly Gly Gly Ser 270 Gly Phe Thr Ala Ala Ala Phe Asp Giu Val Gly Ala 160 Ala Thr Lys Thr Val 240 Ala Arg Gly Ala WO 01/00843 WO 0100843PCTIiBOO/00923 Gly Gin Ala Arg Ile Leu Leu Ala Ilie Ala Ile Ala Thr Gly Ala His 305 310 315 320 Pro Val Thr Leu Tyr 325 <210> 127 <211> 775 <212> DNA <213> Corynebacterium <220> <221> CDS <222> (101)..(775) <223> RXN03003 <400> 127 tcgatgatct gggctcccca g iut aiicum ggtggtgcgg ctaagcttgg accacaagat tttgatcacc caatgatcgc tgcgctgccg cctcaggcat aatctaacgc atg acc tct cgc Met Thr Ser Arg acc Thr 115 163 ccg ctt gtt tct gtt ctt cct gat ttt Pro Leu Val Ser Val Leu Pro Asp Phe ccg Pro 15 tgg gat tcg ctc Trp Asp Ser Leu gct tcc Ala Ser gca aaa gcc Ala Lys Ala gct gcg tct cac Ala Ala Ser His gat ggg atc gtg Asp Gly Ile Vai aat ctt tct Asn Leu Ser gtt ggc act ccg gtt gat ccg gtc gcg ccc agc att cag atc gcg ttg Vai Giy Thr Pro Vai Asp Pro Vai Aia Pro Ser Ile Gin Ile Ala Leu gca gaa Ala Giu gca gcg ggg ttt Ala Ala Giy Phe ggt tac cct caa Gly Tyr Pro Gin atc ggc acc ccg Ile Giy Thr Pro gaa ctc cgc gca gcc atc Giu Leu Arg Ala Ala Ile agg ggc gcg ctt Arg Gly Ala Leu cgg cgc tac aac Arg Arg Tyr Asn aca aag ctt gtc Thr Lys Leu Val gcc tcc ctc ctc Ala Ser Leu Leu ccc Pro gtc gtg ggt acc Vai Vai Gly Thr aag gag Lys Giu 100 259 307 355 403 451 499 547 gca att gcc ctt ctt cca ttc gcg Ala Ile Ala Leu Leu Pro Phe Ala 105 atc cca gag att gcg tac cca acc Ilie Pro Giu Ile Ala Tyr Pro Thr 120 125 ggt att tcc ggc Gly Ile Ser Gly acc gtt gtc Thr Val Val 115 gtg gcc gca Val Ala Ala tac gaa gtc gct Tyr Giu Val Ala gga tgc Gly Cys 135 acc gtg ttg cgt Thr Val Leu Arg gat tcg ctg ttt aag ctc ggc ccg cag Asp Ser Leu Phe Lys Leu Gly Pro Gin 145 WO 01/00843 WO 0100843PCT/IBOO/00923 atc ccg tcg atg atg ttt atc aac tca cca tcc aac ccc aca ggc aag 595 Ile Pro Ser Met Met Phe Ile Asn Ser Pro Ser Asn Pro Thr Gly Lys 150 155 160 165 gtt ctg ggc atc cca cac ttg cgc aag gtt gtg aag tgg gcg cag gaa 643 Val Leu Giy Ile Pro His Leu Arg Lys Val Val Lys Trp Ala Gin Glu 170 175 180 aac aac gtg atc ctc gca gct gat gaa tgc tac ttg ggt ctt ggc tgg* 691 Asn Asn Val Ile Leu Ala Ala Asp Glu Cys Tyr Leu Gly Leu Gly Trp 185 190 195 gac gat gaa aac cca ccg atc tca att ttg gat cca cgt gtc tgc gat 739 Asp Asp Giu Asn Pro Pro Ile Ser Ile Leu Asp Pro Arg Val Cys Asp 200 205 210 ggc gac cac acc aac ttg atc gcc att cac tcg ctg 775 Gly Asp His Thr Asn Leu Ile Ala Ile His Ser Leu 215 220 225 <210> 128 <211> 225 <212> PRT <213> Corynebacterium glutarnicuin <400> 128 Met Thr Ser Arg Thr Pro Leu Val Ser Val Leu Pro Asp Phe Pro Trp 1 5 10 Asp Ser Leu Ala Ser Ala Lys Ala Lys Ala Ala Ser His Pro Asp Gly 25 Ile Val Asn Leu Ser Vai Gly Thr Pro Val Asp Pro Val Ala Pro Ser 40 Ile Gin Ile Ala Leu Ala Giu Ala Ala Gly Phe Ser Giy Tyr Pro Gin 55 Thr Ilie Gly Thr Pro Giu Leu Arg Ala Ala Ile Arg Gly Ala Leu Giu 70 75 Arg Arg Tyr Asn Met Thr Lys Leu Val Asp Ala Ser Leu Leu Pro Val 90 Val Gly Thr Lys Giu Ala Ile Ala Leu Leu Pro Phe Ala Leu Gly Ile 100 105 110 Ser Gly Thr Vai Val Ilie Pro Glu Ile Ala Tyr Pro Thr Tyr Giu Val 115 120 125 Ala Val Val Ala Aia Gly Cys Thr Val Leu Arg Ser Asp Ser Leu Phe 130 135 140 Lys Leu Gly Pro Gin Ile Pro Ser Met Met Phe Ile Asn Ser Pro Ser 145 150 155 160 Asn Pro Thr Gly Lys Val Leu Giy Ile Pro His Leu Arg Lys Val Val 165 170 175 Lys Trp Ala Gin Giu Asn Asn Vai Ile Leu Ala Ala Asp Giu Cys Tyr WO 01/00843 WO 0100843PCT/IBOO/00923 Leu Gly Leu Gly Trp Asp Asp Glu Asn 195 200 Pro Arg Val Cys Asp Gly Asp His Thr 210 215 Pro Pro Ile Asn Leu Ile 220 Ser 205 Ile Leu Asp Ala Ile His Ser <210> <211> <212> <213> <220> <221> <222> <223> 129 1206

DNA

Corynebac t e r iu

CDS

(101)..(1183) RXN00 508 giutanicun <400> 129 agccacgtgt ttaaggatag ttgaaagcgt ggggcaatac tggcactaac cccggcacca atcgtatttc tgtccgcggt tggtggcaca atagttc acc aaa att gac ctg gat gcc atc gcc cat Thr Lys Ilie Asp Leu Asp Ala Ilie Ala His 15 aac atg aac ttg ctg Met Asn Leu Leu 1 aac acg agg gtg ctt Asn Thr Arg Val Leu aaa Lys caa atg gcg ggt ccg gcg aag ctg Gin Met Ala Gly Pro Ala Lys Leu atg Met 30 gcg gtg gtg aag Ala Vai Val Lys gcg aat gca Ala Asn Ala gct cat ggt Ala His Gly tat aac cat Tyr Asn His ggc gta gag aag Gly Val Giu Lys gct ccg gtt att Ala Pro Val Ile gcg gat Ala Asp gcg ttt ggt gtg Ala Phe Gly Val act ctt gcg gag Thr Leu Ala Giu atg cag ttg cgt Met Gin Leu Arg gat atc ggc atc agc ASP Ile Giy Ilie Ser gag gtt ttg tgt Glu Val Leu Cys att tgg aca ccg Ile Trp Thr Pro cag gat ttc cgc gcc gcc att gat cgc Gin Asp Phe Arg Ala Ala Ile Asp Arg aa t Asn att gat ttg gct Ilie Asp Leu Ala gtt att Val Ile 100 tct ccc gcg cat gcc aaa gcc ttg Ser Pro Ala His Ala Lys Ala Leu 105 atc Ile 110 gaa act gat gcg gag cat att GiU Thr ASP Ala Glu His Ile 115 cgg gtg tcc Arg Val Ser 120 atc aag att gat Ile Lys Ile Asp ggg ttg cat cgt Gly Leu His Arg t cg Ser 130 ggt gtg gat Gly Val Asp WO 01/00843 gag cag gag tgg gag ggc gtg ttc agc Giu Gin Giu Trp Giu Gly Val Phe Ser 135 att gag Ile Giu 150 gag aat Giu Asn gcg ctc Ala Leu aac tca Asn Ser cga ccg Arg Pro 215 cat ggt His Giy 230 aag caa Lys Gin gct gag Aia Glu ggc atg Gly Met ctg gac 1027 Leu Asp 295 tct ttg 1075 Ser Leu 310 ata ttc 1123 Ile Phe gac acc 1171 Asp Thr atg Met 155 gat Asp c ac His t tg Leu ttt Phe gcg Aia 235 gga G ly ttt Phe gcc Al a gtt Vai cca Pro 315 ggg Gly gag Glu 140 ttc Phe cgc Arg ggg Gly act Thr tat Tyr 220 a tg Met caa Gin gtg Vai cag Gin ggg Gly 300 c ac His cat His gta Val cac His att Ile gag Giu 190 tct Ser t tg Leu tgg Trp, act Thr gtg Val1 270 aaa Lys gta Val gtg Vali gca Ala tgc Cys 350 gcg Ala t tg Leu att Ile 175 tgc Cys ga t Asp gaa Giu gag Giu tcc Ser 255 gtg Val1 ttc Phe tgc Cys gaa Glu act Thr 335 cga Arg ttg gct gct gcc Leu Ala Ala Ala gcg Ala 160 gc t Aia ccg Pro tta Leu ccc Pro gcg Ala 240 tat Tyr cc t Pro tcc Ser atg Met gct Ala 320 gat Asp cca Pro 145 tgc Cys ttt Phe gtc Vali cac His gtg Val 225 aag Lys ggc Gly gcg Ala gtc Val1 gat Asp 305 ggg Gly ttt Phe acc Thr gat Asp cgc Arg cac His 195 gag Glu gga Gly agc Ser acc Thr tat Tyr 275 att Ile ttc Phe aag Lys gag Giu cga Arg 355 PCTJIBOO/00923 ccg cac 547 Pro His gag cca 595 Giu Pro 165 gcc ctt 643 Ala Leu 180 gta tgc 691 Val Cys atg gtc 739 Met Val ctg gag 787 Leu Glu gtc gta 835 Val Vai 245 tgg cgc 883 Trp Arg 260 gcc gat 931 Ala Asp gat ggc 979 Asp Gly gtt att Vai Ile gcc gtg Ala Val 325 cgt tta Arg Leu 340 act gtc Thr Val WO 01/00843 cgc gca tat gtt taagtgaata cgtttaagga gca 1206 Arg Ala Tyr Val 360 <210> 130 <211> 361 <212> PRT <213> Corynebacterium giutamicun <400> 130 PCTIIBOO/00923 Met Asn Leu Leu Thr Thr Lys Ile Asp Val1 Ala Ala Gin Thr Ala Giu 115 Gly Ala Asp Arg His 195 Giu Gly Ser Leu Asn His Leu Pro Val1 100 His Val1 Pro Glu Ala 180 Vali Met Leu Val1 Lys Ala Gly Arg Giu Ile Ile Asp His Pro 165 Leu Cys Val1 Glu Val1 245 Leu Pro Val1 Gly Ser Ala Ala Lys Glu Giy Thr 170 Lys Phe Ala Pro Ala 250 Asp Ala Glu Val Gin Ala Lys Ile Gly Met 155 Asp His Leu Phe Ala 235 Gly Ala Lys Lys Ala Giu Ile Ala Asp Val1 140 Phe Arg Gly Thr Tyr 220 Met Gin Ile Leu Val Thr Val

ASP

Leu Ser 125 Phe Thr Gin Leu Arg 205 Gly Thr Gly Gly Leu Thr Trp Arg Ala Glu Asp Arg Gly Phe Val Ala Val Val Pro WO 01/00843 WO 0100843PCTIBOOOO923 Ala Val Asp 305 Gly Phe Thr Tyr 275 Ile Phe Lys Giu Arg 355 Gly Leu Ser 310 Ile Asp Arg Pro 280 Tyr Gly Gly Ile Tyr 360 265 Arg His Ala Gin Gly 285 Pro Gin Val Gly Arg 300 Asp Asn Pro His Gly 315 Glu Asn Giy His Asp 330 Asn Tyr Giu Vai Val 345 Val Phe Cys Giu Thr 335 Arg <210> 131 <211> 1152 <212> DNA <213> Corynebacteriun giutamicun <220> <221> CDS <222> (101)..(1129) <223> RXN00636 <400> 131 tcatgggatt cagcaaggcg gccacccgaa ccgtctttgg tcgatctggc ccgttcgaac ataaggaata ttcctactcc ctc Leu cac His aaa Lys atc Ile ttt Phe gtg Val1 att Ile gcc Ala atc Ile acc Thr acg Thr caa Gin tta Leu 15 at t Ile cag Gin Giy gca Ala atc Ile 95 act Thr gcc Ala atg Met gag Giu tat Tyr ccc Pro tggaggagta ggagcgatga atg atg att gat aca Met Met Ile Asp Thr 1 gcc aac att tcc agg Aia Asn Ile Ser Arg ctg cgt ccg cat gtg Leu Arg Pro His Vai cag gtc gac gcc ggt Gin Vai Asp Ala Gly gcg gaa att ttt gcc Ala Glu Ile Phe Ala ccg ctg tat cta acc Pro Leu Tyr Leu Thr gga gaa att tcc att Gly Giu Ile Ser Ile 100 WO 01/00843 WO 0100843PCT/EBOO/00923 ggc gtg Gly Val gat atc Asp Ile gtc acg Val Thr 135 agc agg Ser Arg 150 gga aat Giy Asn agc gtc Ser Val tct gcg Ser Ala gtg ttt Val Phe 215 cag gtg Gin Val 230 gat cgt Asp Arg aaa cca Lys Pro gcc cga Ala Arg gat aaa 1027 Asp Lys 295 aac cat 1075 Asn His 310 gaa gcc 1123 gat Asp aag Lys 120 gcg Ala tat Tyr gg t Gly cag Gin cag Gin 200 aac Asn gca Al a cgg Arg gca Ala atc Ile 280 gtg Val1 gcc Ala gat tcg Ser 105 gc t Al a act Thr gca Ala gag Glu cga Arg 185 ttt Phe gat Asp atg Met atc Ile tgg Trp 265 tct Ser cta Leu tgc Cys ggC gta Val ctg Leu gct Ala gga Gly cag Gin 170 ctt Leu aca Thr t cc Ser acg Thr att Ile 250 att Ile gc t Ala ctt Leu aac Asn act atg Met gaa Giu gaa Giu 140 ttt Phe gca Ala ggc Gly gca Ala cag Gin 220 ctg Leu gat Asp ggc Gly tcg Ser gta Val1 300 att Ile cgt cag Gin 110 ga t Asp agt Ser ttt Phe gat Asp c tg Leu 190 gat Asp acc Thr act Thr gga Gly ggt Gly 270 cat His ggg Gly ttg Leu tgg gcg Ala tcg Ser cag Gin cct Pro gag G iu i7 5 act Thr gag Giu tcg Ser gtg Val t cc Ser 255 ttt Phe cac His gag Glu gtg Val1 aag acg gcg Thr Ala gga cat Gly His atc cgc Ile Arg 145 ggg cat Giy His 160 ctt cag Leu Gin tct ggc Ser Giy atg cga Met Arg gga gca Gly Ala 225 gtc agc Val Ser 240 aaa atc Lys Ilie gtt ctg Val Leu gca acc Aia Thr cag ctc Gin Leu 305 gat gag Asp Giu 320 gta gtt ggt Gly cg t Arg 130 gag Giu tct Ser gc t Ala gg t Gly cca Pro 210 tgc Cys cga Arg ctc Leu ggg Gly att Ile 290 aac Asn gtc Val1 gcc t tg Leu 115 aga Arg gcg Ala tat Tyr cta Leu tcc Ser 195 ggc G ly act Thr aa t Asn agc Ser aat Asn 275 ttc Phe atc Ile t ac Tyr cgc cgg Arg agt Ser ctg Leu ggc Gly aac Asn 180 tcg Ser gtg Val1 gag Giu gtg Val act Thr 260 cc t Pro tgg Trp gtg Val gtt Val1 ggc gaa Giu gga Gly ggc Gly ccg Pro 165 aac Asn ccg Pro tat Tyr aag Lys t ca Ser 245 gat Asp gaa Giu cca Pro ccc Pro cgg Arg 325 aga 451 499 547 595 643 691 739 787 835 883 931 979 WO 01/00843 PCTJIIBOO/00923 Giu Ala Asp Gly Thr Phe Arg Thr Trp Lys Val Val Ala Arg Gly Arg 330 335 340 aac aat tagggaaacc tcttgacctt cac 1152 Asn Asn <210> 132 <211> 343 <212> PRT <213> Corynebacterium giutamicum <400> 132 met Met Ile Asp Thr Pro Ala Val Leu 1 Ala Leu Gin Ala Pro Gly Ala H-i s Arg 145 His Gin Gly Arg Ala 225 Ser Asn Arg Val1 Giu Leu Giu Gly Arg 130 Giu Ser Ala Gly Pro 210 Cys Arg Ile Pro Asp Ilie Tyr Ilie Leu 115 Arg Al a Tyr Leu Ser 195 Gly Thr Asn Ala His 40 Gly Gly Ala Asp Lys 120 Ala Tyr Gly Gin Gin 200 Asn Ala Arg Ile 10 Al a Ile Thr Thr Gin Val Leu Ala Gly Gin 170 Leu Thr Ser Thr Ile Asp Gly Ile Cys Asp 75 Arg Giu Ile Ser Val 155 Al a Ala Asp Gin Val1 235 Leu Arg Ala Giu Ala Ile Leu Met Giu Giu 140 Phe Ala Gly Ala Gin 220 Leu Asp Giu His Ile Thr Phe Asn Ala Val 125 Leu Thr Ala Gly Ile 205 Ile Ser Ala Leu Ile Gin Gly Ala Ile Al a Ser Gin Pro Giu 175 Thr Giu Ser Val1 Ser Th r Al a Met Glu Tyr Pro Thr Gly Ile Gly 160 Leu Ser Met Gly Val1 240 Lys WO 01/00843 Ile Leu Thr Leu 305 Giu Val1 Lys Ala Asp Asn 310 Giu Asn Pro Ala Trp 265 Arg Ile Ser 280 Lys Val Leu 295 His Ala Cys Ala Asp Gly Asn 250 Ile Asp Gly Asn Gly 270 Ala Leu Ser Giu His 285 Leu Pro Val Ile Gly 300 Asn Val Ile Asn Leu 315 Thr Phe Arg Thr Trp, 330 PCTIiBOO/00923 255 Phe Val His Ala Giu Gin Val Asp 320 Lys Val 335 <210> 133 <211> 879 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (101)..(856) <223> RXA02536 <400> 133 aagaagtgat cacgcgaacc tgtgtataac ttgcctcaaa gcgtattgcc ttgcttcaga tctcgacgaa ttccgataag ctg Leu ttg Leu gat Asp aaa Lys cc t Pro aac Asn gct Ala gcg Ala gag Glu ctg Leu cag Gin 75 agt Ser gaa Giu act Thr ctc Leu gac Asp cgc Arg ggc Gly gcgcctaggc tgtggattat atg gac aac ttc gcc Met Asp Asn Phe Ala 1 cag ggg gct cgg gtg Gin Gly Ala Arg Val ggt acg gga agg ctt Gly Thr Gly Arg Leu tcc acc gcg gta cga Ser Thr Ala Val Arg gcg ggc atg ttc acc Ala Gly met Phe Thr atc tcg cgc gtc aac Ilie Ser Arg Val Asn cag gga tac aac aaa Gin Gly Tyr Asn Lys 100 115 163 211 259 307 355 403 WO 01/00843 WO 0100843PCT/EBOO/00923 att Ile ccg Pro gtg Val gcc Ala 150 ggt Gly ctg Leu gaa Giu atg Met cca Pro 230 cgg Arg cac His ggc Gly gcg Ala 135 cgc Arg cct Pro gat Asp' gaa Giu gtg Val 215 gaa Giu gag Glu gac Asp ctg Leu tac Tyr gca Ala tta Leu 170 tgc Cys ga t Asp c ca Pro atc Ile gcg Ala gtt Val ga t Asp cag Gin 155 gaa Glu tgg Trp gaa Giu cac His gcg Ala 235 agg Arg gtc Val cca Pro gtt Val gtc Val1 175 tgt Cys cc t Pro att Ile gtc gac Asp att Ile 130 t tc Phe tcg Ser cgc Arg gcg Ala att Ile 210 gcg Ala t tg act Thr 115 aaa

LYS

aaa

LYS

tgg Trp gcg Ala cga Arg 195 gg t Gly gg t Gly gcc gtg Val t tt Phe gac Asp caa Gin cgt Arg 180 ctt Leu cat His tat Tyr aaa Lys 451 499 547 595 643 691 739 787 835 Ile Asp Val Ser Gly Leu Ala 240 taaccactgt ctaaggaatc act ttg cct gtt ctt Leu Pro Val Leu 250 <210> 134 <211> 252 <212> PRT <213> Corynebacterium giutanicuni <400> 134 Met Asp Asn Phe Ala Leu Leu Arg 1 5 Gin Gly Ala Arg Val Leu Val Phe Gly Thr Gly Arg Leu Asp Thr Gin 40 Ser Thr Ala Val Arg Lys Leu Ala 1 55 Ala Gly Met Phe Thr Pro Ala Asp ksp ?ro 25 ~sp r'hr 206 Ala Ala Giu Lys Ala Ala Glu 10 Giu Ala Thr Ser Gin Ser Phe Giu Giu Leu Asp Gly Giu Phe Giu Leu Asp Val Val Ile Val Val Gin Arg Gly Giu Lys Thr WO 01/00843 WO 0100843PCTIBOOIOO923 Ser Giy Asp Ile 130 Phe Ser Arg Ala Ile 210 Ala Leu Arg Tyr Thr 115 Lys Lys Trp Ala Arg 195 Gly Gly Ala Val Asn 100 Val1 Phe Asp Gin Arg 180 Leu His Tyr Lys Asn Lys Lys Gly Leu Asp 165 Ala Pro Ser Giu Ilie 245 Thr His Gly Ala 135 Arg Pro Asp Giu Val 215 Giu Glu Val1 Thr Asp 120 Thr Asn Gly Ser Leu 200 Thr Met Ala Leu Tyr 105 Giu Cys Gly Lys Thr 185 Arg Asn Leu Leu Ile 90 Asp Leu Tyr Ala Leu 170 Cys Asp Pro Ile Pro 250 Ser Ala Val1 Asp Gin 155 Glu Trp Giu His Ala 235 Val Gly Leu Tyr Arg 110 Giu Val Phe Pro Val Val Giu Val 175 Ala Cys 190 Gly Pro Val. Ile Asp Val His Giu Asp Giu Pro 160 Leu Gly Thr Ala Ser 240 <210> 135 <211> 1635 <212> DNA <213> Corynebacteriun giutamicun <22 0> <221> CDS <222> (101)..(1612) <223> RXS00870 <400> 135 caagacggcg atgtcgccgc cgctgttgat accgcagcgc gacttgttca cacagatatt caacaattca cttcgcagag catttaagga atttacacac atg tct gaa cca caa 115 Met Ser Giu Pro Gin 1 acc atc tcg cac tgg att gac ggc gcg att tcc cca tcc act tcc ggc 163 Thr Ile Ser His Trp Ile Asp Gly Ala Ilie Ser Pro Ser Thr Ser Gly 15 aag acc gct cct gtc tac aat cct gca act ggc cag gtc acc gcc aat 211 Lys Thr Ala Pro Val Tyr Asn Pro Ala Thr Gly Gin Val Thr Ala Asn 30 WO 01/00843 WO 0100843PCTIiBOO/00923 gtt Val acc Thr gc t Ala c tg Leu atg Met t tc Phe att Ile agc Ser 150 gca Ala cct Pro cca Pro ggt Gly acc Thr 230 cgc Arg gat Asp gcg Ala aag Lys gtg Val gcg Ala ggt Gly cca Pro gat Asp 135 ccg Pro atc Ile tcg Ser gac Asp t tg Leu 215 cca Pro gtc Val gct Ala ctg Leu gc t Ala ctt Leu gag Giu gaa Glu cac His 120 gtg Val1 ttc Phe gc t Ala gca Ala ggc Gly 200 ctg Leu atc Ile cag Gin gat Asp gct agc cag gaa gag atc gat gcc acc atc gct Ala Ser Gin Giu Giu Ile Asp Ala Thr Ile Ala gc t Al a ttc Phe atc Ile atc Ile 105 ctg Leu tat Tyr aac Asn gca Ala gcg Ala 185 gta Val1 aac Asn gca Ala gcg Ala ctg Leu 265 aag Lys aac Asn atc Ile c tg Leu ctt Leu tcc Ser ttc Phe ggC Gly 170 c tg Leu ttc Phe agc Ser aag Lys t tg Leu 250 ga t Asp acg Thr ttc Phe 75 act Thr cgc Arg aaa Lys ttg Leu cc t Pro 155 aac Asn tgg Trp, aac Asn cc t Pro tac Tyr 235 ggc Gly ctg Leu tgg Trp, 60 cgt Arg gca Ala ggC Gly ggt Gly aag Lys 140 gcg Ala gca Ala atg Met gtg Val1 gat Asp 220 atc Ile G ly gtt Val1 ggc Gly gag Giu gag Giu cag Gin gcg Al a 125 cag Gin atg Met gt t Val1 gc t Ala ctc Leu 205 gtc Vai tac Tyr gcg Ala gcc Al a aac Asn ctg Leu cac His gaa Giu 110 ttc Phe cc a Pro gtg Val1 att Ile cag Gin 190 cag Gin tct Ser gag Giu aag Lys gat Asp 270 ctg Leu ctg Leu ggc Gly 95 gtc Val1 aac Asn ctg Leu ccg Pro ttg Leu 175 atc Ile 9gc Gly gcg Ala act Thr aac Asn 255 cag Gin tct Ser aat Asn 80 aag Lys gtg Val1 gag Giu gg t G ly atg Met 160 aag Lys tgg Trp gac Asp att Ile tcc Ser 240 c ac His gca Ala atc Ile 65 gct Ala gtc Val1 gag Glu aac Asn gtt Vai 145 tgg Trp cct Pro aag Lys aag Lys tcc Ser 225 gcg Ala atg Met atc Ile gc t Ala cgc Arg t tg Leu ctt Leu gtc Val1 130 gtc Val1 ttt Phe tca S er gaa Glu ctg Leu 210 t tc Phe aag Lys ctg Leu aac Asn aag Lys aag Lys tcc Ser gct Ala 115 tcc Ser gg t Gly t tc Phe gag Giu gc t Ala 195 gc t Ala gtg Val aac Asn gtg Val gca Ala 275 tc t Ser cgc Arg ggt Gly gat Asp 100 acc Thr acc Thr atc Ile cca Pro aag Lys 180 ggt Gly gtt Val1 ggt Gly ggc Gly ctg Leu 260 ggt Gly gcc Ala caa Gin gag Giu gcc Ala gg t Gly 9gc Gly atc Ile atc Ile 165 ga t Asp ctt Leu gat Asp tcc Ser aag Lys 245 cca Pro tac Tyr 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 ggc gct gcc ggt gag cgt tgc atg gct gtt tct gtg gtc ttg gct att WO 01/00843 Gly Ala Ala 280 gaa tct gtt 1027 Giu Ser Val 295 PCT/EBOO/00923 Gly Glu Arg Cys gcc gac gag ctc Ala Asp Glu Leu 300 atc ggc aac ggt Ile Gly Asn Gly Ala Val Ser gag aag atc Glu Lys Ile ggc gac gag Giy Asp Giu acc ctg 1075 Thr Leu 310 ctg ggc 1123 Leu Gly gtc gac 1171 Vai Asp aac tgc 1219 Asn Cys ctt atc 1267 Leu Ile 375 ttc ggc 1315 Phe Gly 390 att gag 1363 Ile Glu acc aac 1411 Thr Asn ggc atg 1459 Gly Met tcc ttc 1507 Ser Phe 455 ggc act 1555 cgc Arg gcc Ala Val Val Leu Ala Ile 290 aag gag cgc atc gac Lys Glu Arg Ile Asp 305 cag ggc gag ccg cac Gin Gly Glu Pro His 325 aag gtc gct tct tat Lys Val Ala Ser Tyr 340 atc gtg gac ggg cgt Ile Val Asp Giy Arg cca cta atc Pro Leu Ile 315 320 acc gac gtc cac cgc Thr Asp Val His Arg 335 gcc gac ggc gcc aag Ala Asp Gly Ala Lys gac Asp atc Ile atc gct Ilie Ala 345 gcc gta Ala Val gac ggg cac gag Asp Gly His Glu 365 atc cca. ctc acg Ile Pro Leu Thr 350 355 gag ggc ttc ttc ttc ggc cct acg Giu Giy Phe Phe Phe Gly Pro Thr 370 ttc cgc gcc tac acc gaa gaa atc Phe Arg Ala Tyr Thr Giu Giu Ile 385 gac Asp 380 ccg gtc ctc tct Pro Val Leu Ser 395 ctg atc aac tcc Leu Ile Asn Ser 410 gtc gtt cgt gtc gca Val Val Arg Val Ala 400 ggt gaa ttc ggc aac Gly Giu Phe Gly Asn 415 tcc ttc gac gag gca Ser Phe Asp Glu Ala 405 gga acc gca atc ttc Gly Thr Ala Ile Phe 420 cat gag atc gaa gtg His Glu Ile Glu Val gat ggt Asp Gly 425 atc ggc Ile Gly gga gcg gca cgc cgc Gly Ala Ala Arg Arg 430 atc aac gta cca atc Ilie Asn Val Pro Ile 445 tgg aag aac tcc ctc Trp Lys Asn Ser Leu ttc cag Phe Gin 435 cca gtg cct gtt gcg tac cac Pro Val Pro Val Ala Tyr His gg t Gly ttc ggt gac Phe Gly Asp 465 450 gcc aag gca tat Ala Lys Ala Tyr 460 caa ggt ttt gat ttc ttc acc agg gaa aag gcg atc acc agc Arg Glu Lys Ala Ile Thin Ser Gly Thin Gin Gly Phe Asp Phe Phe Thr WO 01/00843 470 cgt tgg ctc 1603 Arg Trp Leu cag aac gat 1635 Gin Asn Asp PCTIIBOO/00923 475 480 485 gac cca gca acc cac ggt ggc att aac ctc ggt ttc cca Asp Pro Ala Thr His Gly Gly Ile Asn Leu Giy Phe Pro 490 495 500 taattgaagg agagcacagg act <210> 136 <211> 504 <212> PRT <213> Corynebacterium giutamicum <400> 136 Met Ser Glu Pro Gin Thr Ile Ser His 1 Pro Gin Thr Ile Ala Val1 Giu Asn Val1 145 Trp Pro Lys Lys Lys Val1 Thr Ala Leu Met Phe Ile Ser 150 Ala Pro Pro Gly Thr Ala Lys Val1 Ala Gly Pro Asp 135 Pro Ile Ser Asp Leu 215 Al a Leu Ala Leu Giu Glu His 120 Val1 Phe Ala Ala Gly 200 Leu Trp 10 Val Ser Lys Asn Ile Leu Leu Ser Phe Gly 170 Leu Phe Ser Asp Asn Glu Trp Arg Ala Gly Gly Lys 140 Ala Ala Met Val1 Asp 220 Gly Pro Giu Gly Giu Giu Gin Ala 125 Gin Met Val1 Ala Leu 205 Val Ala Ala Ile Asn Leu His Giu Phe Pro Val1 Ile Gin 190 Gin Ser Ile Thr Asp Leu Leu Giy Val Asn Leu Pro Leu 175 Ile Gly Ala WO 01/00843 Ser Phe Val 225 Ala Lys Asn Met Leu Val Ile Asn Ala 275 Val Val Leu 290 Lys Giu Arg 305 Gin Gly Giu Lys Vai Aia Ile Val Asp 355 Phe Phe Gly 370 Tyr Thr Glu 385 Ser Phe Asp Giy Thr Ala His Giu Ile 435 Pro Val Ala 450 Asp Ala Lys 465 Lys Ala Ile Gly Gly Leu 260 Giy Ala Ile Pro S er 340 G ly Pro Giu Giu Ile 420 Glu Tyr Ala Thr Ser Lys 245 Pro Tyr Ile Asp His 325 Tyr Arg Thr Ile Ala 405 Phe Val1 His Tyr Ser 485 Thr 230 Arg Asp Gly Giu Thr 310 Leu Val Asn Leu Phe 390 Ile Thr Gly Ser Gly 470 Arg Pro Val Ala Ala Ser 295 Leu Gly Asp Cys Ile 375 Giy Giu Asn Met Phe 455 Thr Trp Ala Ala Leu 265 Gly Ala Ile Leu Ala 345 Val Asp Val1 Ile Gly 425 Gly Gly Gly Asp Lys Leu 250

ASP

Giu

ASP

Gly Ile 330 Giu Asp Ile Leu Asn 410 Gly Ile Trp Phe Pro 490 Tyr 235 Gly Leu Arg Glu Asn 315 Thr Ala Gly Pro Ser 395 Ser Ala Asn Lys Asp 475 Ala Ile Gly Val1 Cys Leu 300 G ly Asp Asp His Leu 380 Val Gly Ala Val Asn 460 Phe Thr Tyr Ala Ala Met 285 Ile Ala Val1 Gly Glu 365 Thr Val1 Giu Arg Pro 445 Ser Phe His Giu Lys Asp 270 Ala Glu Gly His Ala 350 Giu Phe Arg Phe Arg 430 Ile Leu Thr Gly PCTIBOO/00923 Thr Ser 240 Asn His 255 Gin Ala Val Ser Lys Ile Asp Giu 320 Arg Asp 335 Lys Ile Gly Phe Arg Ala Val Ala 400 Gly Asn 415 Phe Gin Pro Val Phe Gly Arg Giu 480 Gly Ile 495 Asn Leu Gly-Phe 500 Pro Gin Asn Asp <210> 137 <211> 531 <212> DNA <213> Corynebacterium glutanicun <220> WO 01/00843 PCTIBOOIOO923 <221> CDS <222> (101)..(508) <223> R.XS02299 <400> 137 acgcgggggt tgttgccgga tcgaaatatt cctttccttg tcatctcacg ctatgatttc taaaacttgc aggacaaccc ccataaggac accacaggac atg ctg cgc acc atc 115 Met Leu Arg Thr Ile 1 ctc gga agt aag att cac cga gcc act gtc act caa gct gat cta gat 163 Leu Gly Ser Lys Ilie His Arg Ala.Thr Val Thr Gin Ala Asp Leu Asp 15 tat gtt ggc tct gta acc atc gac gcc gac ctg gtt cac gcc gcc gga 211 Tyr Val Gly Ser Val Thr Ile Asp Ala Asp Leu Val His Ala Ala Gly 30 ttg atc gaa ggc gaa aaa gtt gcc atc gta gac atc acc aac ggc gct 259 Leu Ile Giu Gly Giu Lys Val Ala Ile Val Asp Ile Thr Asn Gly Ala 45 cgt ctg gaa act tat gtc att gtg ggc gac gcc gga acg ggc aat att 307 Arg Leu Giu Thr Tyr Val Ile Val Gly Asp Ala Gly Thr Gly Asn Ile 60 tgc atc aat ggt gcc gct gca cac ctt att aat cct ggc gat ctt gtg 355 Cys Ilie Asn Gly Ala Ala Ala His Leu Ile Asn Pro Gly Asp Leu Val .75 80 atc atc atg agc tac ctt cag gca act gat gcg gaa gcc aag gcg tat 403 Ilie Ile Met Ser Tyr Leu Gin Ala Thr Asp Ala Glu Ala Lys Ala Tyr 95 100 gag cca aag att gtg cac gtg gac gcc gac aac cgc atc gtt gcg ctc 451 Giu Pro Lys Ile Val His Vai Asp Ala Asp Asn Arg Ile Val Ala Leu 105 110 115 ggc aac gat ctt gcg gaa gca cta cct gga tcc ggg ctt ttg acg tcg 499 Gly Asn Asp Leu Ala Giu Ala Leu Pro Gly Ser Gly Leu Leu Thr Ser 120 125 130 aga agc att tagcgtttta gctcgccaat att 531 Arg Ser Ile 135 <210> 138 <211> 136 <212> PRT <213> Corynebacterium giutarnicum <400> 138 Met Leu Arg Thr Ilie Leu Gly Ser Lys Ile His Arg Ala Thr Val Thr 1 5 10 Gin Ala Asp Leu Asp Tyr Val Gly Ser Val Thr Ile Asp Ala Asp Leu 25 Val His Ala Ala Gly Leu Ile Giu Gly Giu Lys Vai Ala Ile Val Asp 212 WO 01/00843 WO 0100843PCTIBOO/00923 Ilie Thr Asn Gly Ala Arg Leu Glu Thr Tyr Val 55 Gly Thr Gly Asn Ilie Cys Ile Asn Gly Ala Ala 70 Pro Gly Asp Leu Val Ile Ile Met Ser Tyr Leu 90 Glu Ala Lys Ala Tyr Glu Pro Lys Ile Val His 100 105 Arg Ile Val Ala Leu Giy Asn Asp Leu Ala Giu 115 120 Gly Leu Leu Thr Ser Arg Ser Ile 130 135 <210> 139 <211> 1053 <212> DNA <213> Corynebacterium glutanicuin <2 <221> CDS <222> (101)..(1030) <223> RXA0i561 <400> 139 gtgcccagaa attctgcttg cactcaccca agccgtttag taataatgtt cattttcatc gagttctaga aaacacaggc Ile Ala Gin Val1 Ala Asp Ile Asp Asp Gly gat Asp cca Pro gca Aia aac Asn ggc Gly gcc Ala caa Gin ccc Pro tgc Cys t cg Ser ggc G ly gtt Val caaattgaac ctcacgttca atg ctc acc ctc aac Met Leu Thr Leu Asn 1 cat gtt cga cga acg His Val Arg Arg Thr caa atc tgg atc aaa Gin Ilie Trp Ile Lys acg cgt gga gca ttc Thr Arg Gly Ala Phe ctc gac cca acg gtt Leu Asp Pro Thr Val ctc gca aat gct ttt Leu Ala Asn Ala Phe ttg gtg ccc gaa act Leu Val Pro Glu Thr 100 WO 01/00843 WO 0100843PCTIBOO/00923 gcc cca caa gta aaa gtt gat cgc ctc aag caa tac ggt gca acc gtg 451 Ala Pro Gin Val Lys Val Asp Arg Leu Lys Gin Tyr Gly Ala Thr Val 105 110 115 caa caa atc gga tct gaa tat gcg gaa gca ttt gag gca gct caa acc 499 Gin Gin Ile Gly Ser Glu Tyr Ala Glu Ala Phe Glu Ala Ala Gin Thr 120 125 130 ttt gag tcg gaa act ggt gct ctg ttt tgc cac gcc tac gac cag ccc 547 Phe Giu Ser Glu Thr Gly Ala Leu Phe Cys His Ala Tyr Asp Gin Pro 135 140 145 gac atc gca gct gga gca ggc gtc att ggg cta gaa att gtc gaa gat 595 Asp Ilie Ala Ala Gly Ala Gly Val Ile Gly Leu Glu Ile Val Glu Asp 150 155 160 165 ctt ccc gac gtt gac acc atc gtg gtt gct gtc ggt ggc ggt gga ctc 643 Leu Pro Asp Vai Asp Thr Ilie Val Val Ala Val Gly Gly Gly Gly Leu 170 175 180 tat gca gga atc gca gcc gtc gta gca gcc cac gac atc aaa gtg gtg 691 Tyr Ala Gly Ilie Ala Ala Val Val Ala Ala His Asp Ile Lys Val Vai 185 190 195 gcc gtt gaa ccc tcc aaa att cca acc ctg cac aac tca ctc att gcc 739 Ala Val Giu Pro Ser Lys Ile Pro Thr Leu His Asn Ser Leu Ile Ala 200 205 210 ggc caa cca gtc gat gtg aac gtt tct ggt atc gcg gca gat tct ttg 787 Gly Gin Pro Val Asp Val Asn Val Ser Gly Ile Ala Ala Asp Ser Leu 215 220 225 ggg gct cgc caa att gga cga gaa gcc ttt gac atc gca act gcc cat 835 Gly Ala Arg Gin Ile Gly Arg Glu Ala Phe Asp Ile Ala Thr Ala His 230 235 240 245 ccc cca ata ggc gtc cta gtg gac gat gaa gca atc atc gca gct cga 883 Pro Pro Ile Gly Val Leu Val Asp Asp Glu Ala Ile Ile Ala Ala Arg 250 255 260 cgc cac ctc tgg gac aac tac cgc atc cct gcc gag cat ggc gct gcc 931 Arg His Leu Trp, Asp Asn Tyr Arg Ilie Pro Ala Giu His Gly Ala Ala 265 270 275 gca gca ctc gcc tct ctt acc agt gga gca tac aaa cct gca gca gat 979 Ala Ala LeU Ala Ser Leu Thr Ser Gly Ala Tyr Lys Pro Ala Ala Asp 280 285 290 gaa aaa gtg gca gtc att gtg tgc gga gcg aac act gac ctc aca aca 1027 Glu Lys Val Ala Val Ile Val Cys Gly Ala Asn Thr Asp Leu Thr Thr 295 300 305 ctg tgatgtgatt tcaaacgatc aca 1053 Leu 310 <210> 140 WO 01/00843 <211> 310 <212> PRT <213> Corynebacterium giutamicun <400> 140 Met Leu Thr Leu Asn Asp Val Ile Thr PCTIBOO/00923 1 His Gin Thr Leu Leu Leu Tyr Giu Ala 145 Glu Gly Asp Asn Ala 225 Ile Ile Giu Arg Trp Gly Pro Asn Pro Al a 115 Al a Asp Val1 Gly Lys 195 Leu Asp Thr Ala Gly 275 Arg Ile Ala Thr Ala Glu 100 Thr Gin Gin Glu Gly 180 Val Ile Ser Ala Ala 260 Ala Pro Ala Asn Gly 70 Ala Ala Gin Phe Asp 150 Leu Tyr Ala Gly Giy 230 Pro Arg Ala Leu Glu Arg Ile Ala Pro Gin Glu 135 Ile Pro Ala Val1 Gin 215 Ala Pro His Ala Phe Phe 40 Gin Val Ala Gin Ile 120 Ser Ala Asp Gly Glu 200 Pro Arg Ile Leu Leu 280 Gin Asp Lys Ala Se r 75 Ser Val Glu Gly Ala 155 Thr Ala Lys Val Gly 235 Leu Asn Leu Ile Gin Pro Cys Ser Gly Val1 Asp Tyr Ala 140 Gly Ile Val Ile Asn 220 Arg Val1 Tyr Thr Val1 Arg Ile Gly Giu Gly Pro Arg Ala 125 Leu Val1 Val1 Val Pro 205 Val1 Glu Asp Arg Ser 285 Cys Ala Gly Phe Gly Ala Thr Lys Ala Cys Gly Ala 175 Ala Leu G ly Phe Giu 255 Pro Ala Ala Pro Thr Lys Leu Gly Val1 Gin Phe His Leu 160 Val1 His His Ile Asp 240 Ala Ala Tyr Asn Lys Pro Ala Ala Asp Giu Lys Val Ala WO 01/00843 WO 0100843PCTIBOO/00923 Thr Asp Leu Thr Thr Leu 305 310 <210> 141 <211> 1470 <212> DNA <213> Corynebacterium <220> <221> CDS <222> (101)..(1447) <223> RX.A01850 <400> 141 ttcgtgcaac ttcagactct giutamicum tacggaggcg atggaccaaa aacaactaca atcaagcaga tcaccttgta caccaccaga gaaaaggccc accctcagcc atg gct atc agt Met Ala Ile Ser gtt gat cta ttt Vai Asp Leu Phe agc Ser atc ggt atc gga cca tca tcc tca cat Ile Gly Ile Gly Pro Ser Ser Ser His 15 acc gtc Thr Val ggc ccc atg Gly Pro Met cat gtc gat His Val Asp gcc gcc ctc acg Ala Ala Leu Thr atc tct gaa ttt ccc agc tcg Ile Ser Glu Phe Pro Ser Ser atc acg ttg cac Ilie Thr Leu His tcc ctt gcc gcc Ser Leu Ala Ala acc Thr ggt aaa ggc Gly Lys Gly cac tgc His Cys act gac cgg gcg Thr Asp Arg Ala tta ctg ggt ctg Leu Leu Gly Leu gga tgg gaa cca Gly Trp Glu Pro ata gtt ccc att Ile Val Pro Ile gct gca ccc tca ccc ggc gcg ccg att Ala Ala Pro Ser Pro Gly Ala Pro Ile gcg aaa ggt tct Ala Lys Gly Ser gtg Val1 aac ggg cca aag Asn Gly Pro Lys acg gtg tcg tat Thr Val Ser Tyr tcc ctg Ser Leu 100 acc ttt Thr Phe acg ttt gat Thr Phe Asp aaa gga tca Lys Gly Ser 120 cat cct ctt cca gaa cac ccc aat gcc His Pro Leu Pro Glu His Pro Asn Ala 110 acc aca agg act tat ttg tcg gtg ggt Thr Thr Arg Thr Tyr Leu Ser Val Gly 125 ggt Gly 130 ggg ttc att Gly Phe Ilie atg acg Met Thr 135 ttg gag gat ttc cgg aag ctg gac gat atc gga tca ggt gtg Leu Glu Asp Phe Arg Lys Leu Asp Asp Ile Gly Ser Gly Val acc att cat cca Thr Ile His Pro gag Glu 155 gca gag gtg cct tgt cct ttt cag aag agt Ala Glu Val Pro Cys Pro Phe Gin Lys Ser WO 01/00843 WO 0100843PCTIiBOO/00923 tcc caa Ser Gin aat gag Asn Giu gat cga Asp Arg acg ccg Thr Pro 215 cag gta Gin Val 230 gat ctt Asp Leu gaa gaa Glu Giu gct gcg Ala Ala aca ggt 1027 Thr Gly 295 gcg gtg 1075 Ala Val 310 gtg ggg 1123 Val Gly ggg ttg 1171 Gly Leu gcg gag 1219 Ala Giu ggc ggg 1267 tta Leu cgc Arg gtg Val1 200 ggg Gly cac His ga t Asp aac Asn ggg Gly 280 ttt Phe ggc Gly tgt Cys tgt Cys att Ile 360 tta ctc gca tat Leu Ala Tyr cgc gat ttt gcg gag gtc atg Arg Asp Phe Ala Giu Val Met 170 175 tta atc cac ggg gat ctt ggc Leu Ile His Gly Asp Leu Gly 185 190 tgg cag att atg cag gag tgc Trp Gin Ile Met Gin Giu Cys 205 att tta ccg ggt ggg ttg aat Ilie Leu Pro Gly Gly Leu Asn 220 gcg ctg att agc aac ggg gat Ala Leu Ile Ser Asn Gly Asp 235 gct gtg gag tgg gtg aat ctg Ala Val Giu Trp Val Asn Leu 250 255 gcc gct ggt ggt cgt gtg gtt Ala Ala Gly Gly Arg Val Val 265 270 att att ccg gcg gtg atg cac Ile Ile Pro Ala Val Met His 285 ggg gcg gag cag gcg cgg acg Gly Ala Giu Gin Ala Arg Thr 300 atc atc att aag gaa aat gcc Ile Ilie Ile Lys Giu Asn Ala 315 cag ggt gag gtt ggt tca gcg Gin Gly Giu Val Gly Ser Ala 330 335 gca gtc tta ggt ggt tct ccg Ala Val Leu Gly Gly Ser Pro 345 350 gcg ttg gag cac aat ttg gga Ala Leu Giu His Asn Leu Gly 365 gtg cag att ccg tgt att gaa gtg Val1 gca Ala caa Gin 225 tgt Cys gcc Ala gct Ala gcg Ala t tg Leu 305 atc Ile gcg Ala cag Gin acg Thr aac gat Asp caa Gin 210 cgt Arg gag Giu ttg Leu ccg Pro cgg Arg 290 tat Tyr tc t Ser atg Met gtg Val1 tgc Cys 370 gc t gcc Ala 195 ggc Gly cgg Arg ctg Leu Ala act Thr 275 gat Asp acc Thr ggc Gly gcg Ala gaa Giu 355 gat Asp at t aag Lys 180 cat His atc Ile gcg Ala ggt Gly gtg Val1 260 aa t Asn ttt Phe gcg Ala gcg Ala gc t Ala 340 aac Asn ccg Pro gct Gly Gly Leu Val Gin Ile Pro Cys Ile Giu Arg Asn Ala Ile Ala Ala WO 01/00843 375 380 atg aag tcc atc aat gcg gca agg ctt 1315 Met Lys Ser Ile Asn Ala Ala Arg Leu 390 395 aat cgc gtg agt ttg gat gat gtg gtg 1363 Asn Arg Val Ser Leu Asp Asp Val Val 410 cgg gac atg ctg acc aaa tat aag gaa 1411 Arg Asp met Leu Thr Lys Tyr Lys Glu 425 430 acc acc ttg ggc ttc ccg gtg tcg atg 1457 Thr Thr Leu Gly Phe Pro Val Ser Met 440 445 gctttaacac ggc 1470 <210> 142 <211> 449 <212> PRT <213> Corynebacteriun glutamicun <400> 142 Met Ala Ile Ser Val Val Asp Leu Phe PCT/IIBOO/00923 gcc Ala gtc Val1 415 acg Thr acg Thr Ser Ala Thr Arg Ile Val1 His Thr Asp Pro Cgg Arg 400 acg Thr tcc Ser gag Glu Ile Ala Leu Ala Asp Asn Pro Arg Phe Glu 155 ggt gat ggc Gly Asp Gly gct gcc acc Ala Ala Thr 420 ggt ggt ttg Gly Gly Leu 435 tagcggtacg 1 Ser Glu Ala Val1 Gly Val1 Asn Gly Ile 145 Ser Phe Thr Gly Ala Ser Ala Gly 130 Gly WO 01/00843 PCT/IB00/00923 Pro Phe Gin Lys Ser Ser Gin Leu Leu Ala Tyr Gly Arg Asp Phe Ala 165 170 175 Glu Val Met Lys Asp Asn Glu Arg Leu Ile His Gly Asp Leu Gly Thr 180 185 190 Val Asp Ala His Leu Asp Arg Val Trp Gin Ile Met Gin Glu Cys Val 195 200 205 Ala Gin Gly Ile Ala Thr Pro Gly Ile Leu Pro Gly Gly Leu Asn Val 210 215 220 Gin Arg Arg Ala Pro Gin Val His Ala Leu Ile Ser Asn Gly Asp Thr 225 230 235 240 Cys Glu Leu Gly Ala Asp Leu Asp Ala Val Glu Trp Val Asn Leu Tyr 245 250 255 Ala Leu Ala Val Asn Glu Glu Asn Ala Ala Gly Gly Arg Val Val Thr 260 265 270 Ala Pro Thr Asn Gly Ala Ala Gly Ile Ile Pro Ala Val Met His Tyr 275 280 285 Ala Arg Asp. Phe Leu Thr Gly Phe Gly Ala Glu Gin Ala Arg Thr Phe 290 295 300 Leu Tyr Thr Ala Gly Ala Val Gly Ile Ile Ile Lys Glu Asn Ala Ser 305 310 315 320 Ile Ser Gly Ala Glu Val Gly Cys Gin Gly Glu Val Gly Ser Ala Ser 325 330 335 Ala Met Ala Ala Ala Gly Leu Cys Ala Val Leu Gly Gly Ser Pro Gin 340 345 350 Gin Val Glu Asn Ala Ala Glu Ile Ala Leu Glu His Asn Leu Gly Leu 355 360 365 Thr Cys Asp Pro Val Gly Gly Leu Val Gin Ile Pro Cys Ile Glu Arg 370 375 380 Asn Ala Ile Ala Ala Met Lys Ser Ile Asn Ala Ala Arg Leu Ala Arg 385 390 395 400 Ile Gly Asp Gly Asn Asn Arg Val Ser Leu Asp Asp Val Val Val Thr 405 410 415 Met Ala Ala Thr Gly Arg Asp Met Leu Thr Lys Tyr Lys Glu Thr Ser 420 425 430 Leu Gly Gly Leu Ala Thr Thr Leu Gly Phe Pro Val Ser Met Thr Glu 435 440 445 Cys <210> 143 <211> 1425 WO 01/00843 WO 0100843PCTIBOOIOO923 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1402) <223> RXA00580 <400> 143 ttttatatat gggtatcggc ggtctatgct tgtgggcgta ttacgcgcgg gattcgtctt gtgaaaggtt agctgacctg cctgtcccgc gagtgaggtc atg acc gat gcc cac ii! Met Thr Asp Ala His caa gcg gac gat gtc cgt tac cag cca ctg aac gag ctt gat Gin Ala Asp Asp Val Arg Tyr Gin Pro Leu Asn Giu Leu Asp cct gag Pro Glu gtg gct gct Val Ala Ala gag atg atc Giu Met Ile atc gct ggg gaa Ile Ala Gly Giu ctt gcc cgt Leu Ala Arg caa cgc gat aca tta Gin Arg Asp Thr Leu gcg tct gag aac ttc gtt ccc cgt tct Ala Ser Giu Asn Phe Val Pro Arg Ser ttg cag gcg Leu Gin Ala cag ggt Gin Gly tct gtt ctt acc Ser Val Leu Thr aat Asn aag tat gcc gag Lys Tyr Ala Glu tac cct ggc cgc Tyr Pro Gly Arg cgt Arg tac tac ggt ggt Tyr Tyr Gly Gly gaa caa gtt gac atc att gag gat ctt Giu Gin Val Asp Ile Ile Giu Asp Leu cgt gat cgt gcg Arg Asp Arg Ala gct ctc ttc ggt gca gag ttc gcc aat Ala Leu Phe Gly Ala Giu Phe Ala Asn gtt cag Val Gin 100 cct cac tct Pro His Ser gag cca ggc Glu Pro Gly 120 gca cag gct aat Ala Gin Ala Asn gct gct gtg ctg atg act ttg gct Ala Ala Val Leu Met Thr Leu Ala 110 115 ctg tct ttg gct cat ggt ggt cac Leu Ser Leu Ala His Gly Gly His 130 451 gac aag atc atg Asp Lys Ile Met gg t Gly 125 ttg acc Leu Thr 135 cac gga atg aag His Gly Met Lys t tg Leu 140 aac ttc tcc gga Asn Phe Ser Gly aag Lys 145 ctg tac gag gtt Leu Tyr Giu Val gtt Val1 150 gcg tac ggt gtt Ala 'Tyr Gly Val cct gag acc atg cgt gtt gat atg gat Pro GiU Thr met Arg Val Asp Met Asp 160 cag Gin 165 gtt cgt gag att Val Arg Giu Ilie ctg aag gag cag Leu Lys Giu Gin cca Pro 175 aag gta att atc Lys Val Ilie Ilie gct ggc Ala Gly 180 tgg tct gca tac cct cgc cac ctt gat ttc gag gct ttc cag tct att Trp Ser Ala Tyr Pro Arg His Leu Asp Phe Giu Ala Phe Gin Ser Ile WO 01/00843 WO 0100843PCTIiBOO/00923 185 gct gcg gaa gtt Ala Ala Giu Val 200 ggt ctt gtt gct Gly Leu Val Ala ggc gcg aag Gly Ala Lys gtc gat atg Val Asp Met gc t Al a 210 cc t Pro 195 cac ttc gct His Phe Ala tac tct gat Tyr Ser Asp gct ggt Ala Gly cac cca agc cca His Pro Ser Pro 215 gtt gtt Val Val 230 tct tcc act Ser Ser Thr gtc Val 235 cag Gln aag act ttg Lys Thr Leu cct cgt tcc Pro Arg Ser atc att ctg gct Ile Ile Leu Ala aag Lys 250 cag Gin gag tac gcg Giu Tyr Ala ctg aac tct Leu Asn Ser tcc gta Ser Vai 260 ttc cca ggt Phe Pro Gly gct act tct Ala Thr Ser 280 ggt ggt cct Gly Gly Pro cac gca gtt His Ala Val gct gcg aag Ala Ala Lys 275 aag att gct Lys Ile Ala gag cag ttc Glu Gin Phe cgt gac cgt cag Arg Asp Arg Gin 290 cgt ctg act gct Arg Leu Thr Ala gct cgc 1027 Ala Arg 295 tct gat 1075 Ser Asp 310 gtg cac 1123 Val His cag gcg 1171 Gin Ala gcg gtt 1219 Ala Val acg ttg gag ggt gct Thr Leu Glu Giy Ala 300 gcg aag gcc gct ggc Ala Lys Ala Ala Gly 315 ttg gtt ttg gct gat Leu Val Leu Ala Asp 330 gaa gat ctg ctg cac Glu Asp Leu Leu His cgc att ctt gct Arg Ile Leu Ala gtg gat gtc ttg Val Asp Val Leu 320 ctg cgt aac tcc Leu Arg Asn Ser gag Glu 305 acc ggt ggc act gat Thr Gly Gly Thr Asp 325 cag atg gat ggc cag Gln Met Asp Gly Gin 340 335 gag Glu 345 cct ttc Pro Phe gat cct cgt cca Asp Pro Arg Pro 365 gtt ggt atc act gtg aac cgt aac Val Gly Ile Thr Val Asn Arg Asn 350 355 cca atg gtt act tct ggt ctg cgt Pro Met Val Thr Ser Gly Leu Arg 370 cgt ggt ttc gat att cct gca ttc Ara Giv Phe Asp Ile Pro Ala Phe att ggt act 1267 Ilie Gly Thr 375 act gag gtt 1315 Thr Giu Val 390 cct gcg ctg gct Pro Ala Leu Ala 380 gca gac atc att Ala Asp Ile Ile 395 acc 385 ggt act gct ttg Gly Thr Ala Leu 400 gct aat Ala Asn ggt aag tcc Gly Lys Ser 405 WO 01/00843 PCT/IB00/00923 gca gac att gag tct ctg cgt ggc cgt gta gca aag ctt get gca gat 1363 Ala Asp Ile Glu Ser Leu Arg Gly Arg Val Ala Lys Leu Ala Ala Asp 410 415 420 tac cca ctg tat gag ggc ttg gaa gac tgg acc ate gtc taagtttttc 1412 Tyr Pro Leu Tyr Glu Gly Leu Glu Asp Trp Thr Ile Val 425 430 tttgagtttt cat 1425 <210> 144 <211> 434 <212> PRT <213> Corynebacterium glutamicum <400> 144 Met Thr Asp Ala His Gin Ala Asp Asp Val Arg Tyr Gin Pro Leu Asn 1 5 10 Glu Leu Asp Pro Glu Val Ala Ala Ala Ile Ala Gly Glu Leu Ala Arg 25 Gin Arg Asp Thr Leu Glu Met Ile Ala Ser Glu Asn Phe Val Pro Arg 40 Ser Val Leu Gin Ala Gin Gly Ser Val Leu Thr Asn Lys Tyr Ala Glu 55 Gly Tyr Pro Gly Arg Arg Tyr Tyr Gly Gly Cys Glu Gin Val Asp Ile 70 75 Ile Glu Asp Leu Ala Arg Asp Arg Ala Lys Ala Leu Phe Gly Ala Glu 90 Phe Ala Asn Val Gin Pro His Ser Gly Ala Gin Ala Asn Ala Ala Val 100 105 110 Leu Met Thr Leu Ala Glu Pro Gly Asp Lys Ile Met Gly Leu Ser Leu 115 120 125 Ala His Gly Gly His Leu Thr His Gly Met Lys Leu Asn Phe Ser Gly 130 135 140 Lys Leu Tyr Glu Val Val Ala Tyr Gly Val Asp Pro Glu Thr Met Arg 145 150 155 160 Val Asp Met Asp Gin Val Arg Glu Ile Ala Leu Lys Glu Gin Pro Lys 165 170 175 Val Ile Ile Ala Gly Trp Ser Ala Tyr Pro Arg His Leu Asp Phe Glu 180 185 190 Ala Phe Gin Ser Ile Ala Ala Glu Val Gly Ala Lys Leu Trp Val Asp 195 200 205 Met Ala His Phe Ala Gly Leu Val Ala Ala Gly Leu His Pro Ser Pro 210 215 220 WO 01/00843 WO 0100843PCTIIBOO/00923 Val1 225 Gly Leu Ala Phe Glu 305 Thr Gin Thr Thr Asp 385 Ala Pro Pro Asn Val1 Arg 290 Arg Gly Met Val1 Ser 370 Ile Asn Tyr Ser Asp Val 230 Arg Ser Giy Ile 245 Ser Ser Val Phe 260 Ala Ala Lys Ala 275 Asp Arg Gin Ala Leu Thr Ala Ser 310 Gly Thr Asp Val 325 Asp Gly Gin Gin 340 Asn Arg Asn Ala 355 Giy Leu Arg Ile Pro Ala Phe Thr 390 Gly Lys Ser Aia 405 Val Ser Ser Thr Val His LYS Thr Leu Gly 235 240 Lys 250 Gin Lys Giu Ala Leu 330 Leu Asp Ala Asp Ser 410 Gin Gly Ile Gly Ala 315 Aia Leu Pro Leu Ile 395 Leu Giu Gly Ala Ala 300 Gly Asp His Arg Ala 380 Ile Arg Lys His Gin Ala Leu 320 Ser Ile Val1 Phe Leu 400 Ala Lys Leu Ala Ala Asp Tyr Pro Leu Tyr Giu Gly Leu Giu Asp Trp Thr 420 425 430 Ile Val <210> 145 <211> 401 <212> DNA <213> Corynebacteriu' giutamicum <2 <221> CDS <222> <223> RXA01821 <400> 145 cga aac agc caa ggc aaa tgg tgc cca agt acg cga tca cca aaa aat Arg Asn Ser Gin Gly Lys Trp Cys Pro Ser Thr Arg Ser Pro Lys Asn 1 5 10 acc agc atc gaa gac aac ggc gat cac gta gtc atc caa gca ggc gaa Thr Ser Ile Giu Asp Asn Gly Asp His Val Val Ile Gin Ala Gly Glu 223 WO 01/00843 PCTAiB0O/00923 25 gaa acc aca atc gtg gac cgc gtt atc gtc acc acc ggc agc tgg aca 144 Giu Thr Thr Ile Val Asp Arg Val Ile Val Thr Thr Gly Ser Trp Thr 40 agc gag ctc gtg ccc tcc atc gcg cca ctg ctt gaa gtg cga cgc cta 192 Ser Giu Leu Val Pro Ser Ile Ala Pro Leu Leu Giu Vai Arg Arg Leu 55 gtg ctc acc tgg ttc ctg ccc aac aat cca gtg gac ttc caa ccg gaa 240 Val Leu Thr Trp Phe Leu Pro Asn Asn Pro Val Asp Phe Gin Pro Giu 70 75 aac ctg cca tgc ttc atc cgt gac cgt gat ggc ttc cac gta ttt gga 288 Asn Leu Pro Cys Phe Ile Arg Asp Arg Asp Giy Phe Hius Vai Phe Gly 90 gca cca tgc gtc gat ggg tac agc atc aaa att. gcc gga ttg gat gag 336 Ala Pro Cys Val Asp Giy Tyr Ser Ile Lys Ile Ala Gly Leu Asp Giu 100 105 110 tgg ggc gtt cca tta agc ctc gat cca ccg atg tgc cct cgg 378 Trp Gly Val Pro Leu Ser Leu Asp Pro Pro Met Cys Pro Arg 115 120 125 tgatgtcctg atcccggttc cgg 401 <210> 146 <211> 126 <212> PRT <213> Corynebacteriun giutamicum <400> 146 Arg Asn Ser Gin Gly Lys Trp Cys Pro Ser Thr Arg Ser Pro Lys Asn 1 5 10 Thr Ser Ilie GiU Asp Asn Gly Asp His Val Val Ile Gin Ala Gly Giu 25 Giu Thr Thr Ile Val Asp Arg Val Ile Val Thr Thr Gly Ser Trp Thr 40 Ser Giu Leu Val Pro Ser Ile Ala Pro Leu Leu Giu Val Arg Arg Leu 55 Val Leu Thr Trp Phe Leu Pro Asn Asn Pro Val Asp Phe Gin Pro Giu 70 75 Asn Leu Pro Cys Phe Ile Arg Asp Arg Asp Gly Phe His Val Phe Gly 90 Ala Pro Cys Val Asp Gly Tyr Ser Ile Lys Ile Ala Gly Leu Asp Giu 100 105 110 Trp Gly Val Pro Leu Ser Leu Asp Pro Pro Met Cys Pro Arg 115 120 125 <210> 147 WO 01/00843 WO 0100843PCTIBOO/00923 <211> <212> <213> <220> <221> <222> <223> 488

DNA

Corynebacteriun giutainicum

CDS

(101)..(478) RXN02 263 <400> 147 cctgggcaac ccaagtgtat gaaaacgccc tggaaaaagg cgtcggcacc acattgaacc tgtgggaatc acccgcactg gcttgagaga agaaacaaca atg aaa att gcg Met Lys Ile Ala atc ggc ctt gga Ile Gly Leu Giy acc ggc tcc atg gca ctg tgg cac tta Thr Gly Ser Met Ala Leu Trp His Leu agt aac Ser Asn atc cca ggt Ile Pro Giy ggc tac ggc Gly Tyr Gly gag gcc atc ggc Giu Ala Ile Gly gaa caa ttc ggc Giu Gin Phe Gly atc tcc cat Ile Ser His atg gcc tac Met Ala Tyr gca ttc aca ggg Ala Phe Thr Giy gag Giu tcc cga ctg ttt Ser Arg Leu Phe cac gaa His Giu ggc agc acc tac Giy Ser Thr Tyr ccg ttg ctc aaa Pro Leu Leu Lys gca cga gca cta Ala Arg Ala Leu tca tca ctg agc Ser Ser Leu Ser att tcc gga cgc Ilie Ser Gly Arg gaa Glu ctc ttc cac aac Leu Phe His Asn ggt gtc tta agc Gly Val Leu Ser ggc aag gaa gac Giy Lys Giu Asp gca ccc ttc caa Ala Pro Phe Gin cgc ctg Arg Leu 100 gtg gaa tca Val Giu Ser gcg cag atg Ala Gin Met 120 gtg Vai 105 gaa cgt tat gag Giu Arg Tyr Giu cca cat gaa cga Pro His Giu Arg ctt acc gcc Leu Thr Ala 115 cgc agc gtt acc Arg Ser Val Thr cag gtc Gin Vai 125 tagact tccg <210> 148 <211> 126 <212> PRT <2i3> Corynebacterium giutamicum <400> i48 Met Lys Ile Ala Val Ile Gly Leu Giy 1 5 Trp His Leu Ser Asn Ile Pro Gly Val 25 Thr Giy Ser Met Ala Leu Giu Ala Ile Gly Phe Giu Gin WO 01/00843 Phe Gly Ile Phe Arg Met Arg Ala Arg Leu Phe His Pro Phe Gin Glu Arg Leu 115 Gly 40 Gly Ser Leu Ser Met 120 Ala Ser Leu Ser Val1 105 Arg Giu Pro S er Giu Giu Gin 125 PCTIiBOO/00923 Ser Arg Leu Leu Leu Lys Gly Arg Giu Asp Glu Ala Leu Pro His 110 Val1 <210> 149 <211> 460 <212> DNA <213> Corynebacterium glutainicum <22 0> <221> CDS <222> (460) <223> FRXA02263 <400> 149 cctgggcaac ccaagtgtat gaaaacgccc tggaaaaagg cgtcggcacc acattgaacc tgtgggaatc acccgcactg gcttgagaga agaaacaaca atg aaa att gcg gta Met Lys Ile Ala Val ggc Gly cca Pro tac Tyr gaa Giu tca Ser gtc Val1 ctt Leu ggt Gly ggc Gly ggC Gly tca Ser t ta Leu gga Gly gta Val gca Ala agc Ser ctg Leu agc Ser tca Ser gag Glu t te Phe a cc Thr age Ser ace Thr ctg Leu caa Gin c tg Leu aaa Lys gaa Glu gca Ala 1 tgg Trp t tc Phe ttt Phe egc Arg etc Leu ccc Pro cac His gge Gly cgc Arg gca Ala ttc Phe ttc Phe agt Ser tee Ser gc Ala gca Ala aac Asn cgc Arg 100 gtg gaa tca gtg gaa cgt tat gag ctg cca cat gaa cga ctt acc gcc WO 01/00843 PCTIIBOO/00923 Val Giu Ser Val Giu Arg Tyr Glu Leu Pro His Giu Arg Leu Thr Ala 105 110 115 gcg cag atg 460 Ala Gin Met 120 <210> 150 <211> 120 <212> PRT <213> Corynebacteriun glutaxnicum <400> 150 Met Lys Ilie Ala Val Ile Gly Leu Gly Ser Thr Gly Ser Met Ala Leu 1 5 10 Trp His Leu Ser Asn Ile Pro Gly Val Glu Ala Ile Gly Phe Giu Gin 25 Phe Gly Ilie Ser His Gly Tyr Giy Ala Phe Thr Gly Giu Ser Arg Leu 40 Phe Arg Met Ala Tyr His Glu Gly Ser Thr Tyr Val Pro Leu Leu Lys 55 Arg Ala Arg Ala Leu Trp Ser Ser Leu Ser Giu Ile Ser Gly Arg Glu 70 75 Leu Phe His Asn Phe Gly Val Leu Ser Thr Gly Lys Giu Asp Giu Ala 90 Pro Phe Gin Arg Leu Val Giu Ser Val Giu Arg Tyr Giu Leu Pro His 100 105 110 Giu Arg Leu Thr Ala Ala Gin Met 115 120 <210> 151 <211> 1251 <212> DNA <213> Corynebacteriun giutamicum <22 0> <221> CDS <222> (101)..(1228) <223> RXA02176 <400> 151 gggtgctagg aactgacagc ttcagggtta tagttgttgg gtcagatcgt taacgatccc tggccctttt acttccaagc gcagaaagtt gcccgaagac atg acc gac ttc ccc 115 Met Thr Asp Phe Pro 1 acc ctg ccc tct gag ttc atc cct ggc gac ggc cgt ttc ggc tgc gga 163 Thr Leu Pro Ser Giu Phe Ile Pro Gly Asp Gly Arg Phe Gly Cys Gly 15 cct tcc aag gtt cga cca gaa cag att cag gct att gtc gac gga tcc 211 WO 01/00843 Pro Ser Lys gca tcc gtc Ala Ser Val gtg ggt tca Val Gly Ser ggc tac gag Gly Tyr Glu gca gca acc Ala Ala Thr ggt gag ttc Giy Glu Phe ctc gac gag Leu Asp Glu 120 cca cag gca Pro Gin Ala 135 acc tcc act Thr Ser Thr 150 ggc tcc ctg Gly Ser Leu gta gac atc Val Asp Ile ttc gca tcc Phe Ala Ser 200 ctc gag cgc Leu Giu Arg 215 ttc ctc aac Phe Leu Asn 230 tac aac acc Tyr Asn Thr aag tgg atg Val1 atc Ile atc Ile atc Ilie ttC Phe tcc Ser 105 c ca Pro ttC Phe ggc Gly gtt Vai aag Lys 185 gac Asp atc Ilie ctg Leu cca Pro aac Pro acc Thr gag Giu ctt Leu 75 ctc Leu aag Lys atc Ile ggC Gly atg Met 155 at t Ilie tcC Ser ggC Gly aag Lys acc Thr 235 gtt Val1 aac Glu tca Ser gga Gly 60 tcC Ser at t Ile ttc Phe gtc Val1 gcc Ala 140 gt t Val gac Asp ga t Asp ctg Leu atc Ile 220 gca Ala gct Ala ggc Gin cac His 45 ctc Leu cta Leu gaa Glu gca Ala acc Thr 125 gat Asp cca Pro gca Ala gtt Val tgg Trp 205 aac Asn gtg Val1 acc Thr 9gC Ile 30 cg t Arg tcc Ser ggt Giy aag Lys aag Lys 110 gca Ala gtt Val gt t Val acc Thr tac Tiyr 190 Ct t Leu gc t Ala gat Asp ttg Leu c tg Gin cag Gin gac Asp gg t Gly aag Lys gc t Ala gaa Giu att Ile ctt Leu tcC Ser 175 t ac Tyr gca Al a tcc Ser aac Asn ctg Leu 255 gat Ala ccg Pro ctc Leu gcg Ala 80 tcC Ser tc t Ser acc Thr gca Ala cgc Arg 160 ggC Gly t tc Phe gcg Ala gat Asp t CC Ser 240 a tg Met gga G ly Ile Val gca gta Aia Val 50 ttc tcc Phe Ser 65 acc gca Thr Ala ggt cac Gly His aag ctt Lys Leu ggt gac Gly Asp 130 tgg gca Trp Aia 145 ccc gaa Pro Giu gct ggt Ala Giy tcc cca Ser Pro atg agc Met Ser 210 cgc ttc Arg Phe 225 ctg aag Leu Lys ctg gac Leu Asp atg gtt Asp aaa Lys ct t Leu ttC Phe ctt Leu gc t Al a 115 tct Ser cac His ggc Gly gga Gly cag Gin 195 cca Pro atc Ile aac Asn aac Asn gc t PCTIIBOO/00923 Giy Ser aac gtc 259 Asn Val cca gaa 307 Pro Glu tgg gat 355 Trp Asp tct ttc 403 Ser Phe 100 cct tgg 451 Pro Trp ccg gcc 499 Pro Ala aac gaa 547 Asn Giu tct gaa 595 Ser Glu 165 ctg cca 643 Leu Pro 180 aag tgc 691 Lys Cys gca gct 739 Aia Ala cct gag 787 Pro Glu cag acc 835 Gin Thr 245 cag gtc 883 Gln Val 260 cgc acc 931 Lys Trp Met Asn Ser Asn Gly Giy Leu Asp Met Val Ala Arg Thr WO 01/00843 WO 0100843PCTIiBOO/00923 265 270 aca gca agc tcc tcc gcc ctg tac aac tgg Thr Ala Ser Ser Ser Ala Leu Tyr Asn Trp 280 285 gca tcc cca tac gtg gca gat gca gct aag 1027 Ala Ser Pro Tyr Val Ala Asp Ala Ala Lys 295 300 acc atc gac ttc gat gac tcc atc gac gca 1075 Thr Ile Asp Phe Asp Asp Ser Ile Asp Ala 310 315 ctg cgc gca aac ggc atc ctg gac acc gag 1123 Leu Arg Ala Asn Gly Ile Leu Asp Thr Glu 330 335 cgc aac cag ctg cgc atc ggt atg ttc cca 1171 Arg Asn Gin Leu Arg Ile Gly Met Phe Pro 345 350 gtg gaa aag ctc acc gga gca atc gac ttc 1219 Val Giu Lys Leu Thr Gly Ala Ile Asp Phe 360 365 gca agg aag taataccccc actttgaaaa aca 1251 Al'a Arg Lys 375 <210> 152 <211> 376 <212> PRT <213> Corynebacteriun giutamicun <400> 152 Met Thr Asp Phe Pro Thr Leu Pro Ser Giu 1 5 10 Arg Phe Gly Cys Gly Pro Ser Lys Val Arg 25 Ile Val Asp Gly Ser Ala Ser Val Ile Gly 40 Ala Val Lys Asn Val Val Gly Ser Ile Arg 55 Phe Ser Leu Pro Giu Gly Tyr Glu Ile Ile 70 Thr Ala Phe Trp Asp Ala Ala Thr Phe Gly 90 gct Ala cgc Arg gca Ala 320 cc t Pro gcg Ala atc Ile Phe Pro Thr Glu Leu 75 Leu gag Glu tcc S er 305 g tg Val1 t ac Tyr atc Ile ctc Leu Ile Glu Ser Gly Se r Ile gct Ala 290 ctc Leu atc Ile cgc Arg gat Asp ga t Asp 370 Pro Gin His Leu Leu Glu 275 cgc gag Arg Giu gtt gtc Val Val gct aag Ala Lys aag ctg Lys Leu 340 tcc acc Ser Thr 355 ggc ggt Gly Gly Gly Asp Ile Gin Arg Gin Ser Asp Gly Gly Lys Lys gag 979 Giu Gly ata Ile 325 gga G ly gat Asp ttt Phe G ly Ala Pro Leu Ala Ser WO 01/00843 PCT/IB00/00923 Gly His Leu Ser Phe Gly Glu Phe Ser Ser Lys Phe Ala Lys Ala Ser 100 105 110 Lys Leu Ala Pro Trp Leu Asp Glu Pro Glu Ile Val Thr Ala Glu Thr 115 120 125 Gly Asp Ser Pro Ala Pro Gin Ala Phe Glu Gly Ala Asp Val Ile Ala 130 135 140 Trp Ala His Asn Glu Thr Ser Thr Gly Ala Met Val Pro Val Leu Arg 145 150 155 160 Pro Glu Gly Ser Glu Gly Ser Leu Val Ala Ile Asp Ala Thr Ser Gly 165 170 175 Ala Gly Gly Leu Pro Val Asp Ile Lys Asn Ser Asp Val Tyr Tyr Phe 180 185 190 Ser Pro Gin Lys Cys Phe Ala Ser Asp Gly Gly Leu Trp Leu Ala Ala 195 200 205 Met Ser Pro Ala Ala Leu Glu Arg Ile Glu Lys Ile Asn Ala Ser Asp 210 215 220 Arg Phe Ile Pro Glu Phe Leu Asn Leu Gin Thr Ala Val Asp Asn Ser 225 230 235 240 Leu Lys Asn Gin Thr Tyr Asn Thr Pro Ala Val Ala Thr Leu Leu Met 245 250 255 Leu Asp Asn Gin Val Lys Trp Met Asn Ser Asn Gly Gly Leu Asp Gly 260 265 270 Met Val Ala Arg Thr Thr Ala Ser Ser Ser Ala Leu Tyr Asn Trp Ala 275 280 285 Glu Ala Arg Glu Glu Ala Ser Pro Tyr Val Ala Asp Ala Ala Lys Arg 290 295 300 Ser Leu Val Val Gly Thr Ile Asp Phe Asp Asp Ser Ile Asp Ala Ala 305 310 315 320 Val Ile Ala Lys Ile Leu Arg Ala Asn Gly Ile Leu Asp Thr Glu Pro 325 330 335 Tyr Arg Lys Leu Gly Arg Asn Gin Leu Arg Ile Gly Met Phe Pro Ala 340 345 350 Ile Asp Ser Thr Asp Val Glu Lys Leu Thr Gly Ala Ile Asp Phe Ile 355 360 365 Leu Asp Gly Gly Phe Ala Arg Lys 370 375 <210> 153 <211> 1422 <212> DNA <213> Corynebacterium glutamicum <220> WO 01/00843 <221> CDS <222> (101)..(1399) <223> RXN02758 <400> 153 atacatctca cccaattccc cataactaga caattgccca PCT/IBOO/00923 gcaacgactg ataagtctcc aatgtcgtgt tccgcgctca gacatgagac aattgttgcc gtg act gaa ctc atc Val Thr Glu Leu Ile cag Gin gca Al a gac Asp cag Gin aac Asn acc Thr gtg Vai gtt Val1 att Ile ggt Gly 150 ccg Pro gc t Ala ttg Leu tcc S er gaa Glu ggt Gly gtc Val gcg Ala ctg Leu cag Gin 105 gtg Val acc Thr gat Asp agc Ser tc t S er 185 cgt Arg ca a Gin gg t Gly gtg Val1 ttg Leu ttt Phe act Thr gaa Giu ttg Leu ctg Leu tac Tyr cc t Pro 170 gag Giu tct Ser gag Glu gcg Ala 30 t tc Phe cag Gin gca Ala aag Lys tcg Ser 110 gtt Val1 gat Asp ggc Gly gaa Glu gat Asp 190 gtg Val1 ctg Leu 15 gtg Val1 ttt Phe tca Ser cc t Pro gtg Val tcc Ser gat Asp gcc Ala ctg Leu gcg Ala 175 att Ile tgc Cys 1 gcc Ala aca Thr gtc Val1 t tc Phe cgt Arg gga Gly cc t Pro t tg Leu att Ile 145 ctg Leu cgt Arg att Ile gat Asp ggc Gly gtg Val1 ttg Leu cgt Arg gtc Val1 cag Gin cgt Arg gat Asp 130 gac Asp aag Lys aag Lys gag Giu tgt Cys WO 01/00843 PCTIBOOIOO923 200 205 210 ttg atc act ggt gag gtc att gag atg ctg gcg gct cac gcg ggc aag 787 Leu Ile Thr Gly Giu Val Ile Giu Met Leu Ala Ala H-is Ala Gly Lys 215 220 225 gaa gct gaa gtt gcg gca gtt act gag cgt gcg atg cgc ggt gag ctc 835 Giu Ala Giu Val Ala Ala Val Thr Giu Arg Ala Met Arg Gly Giu Leu 230 235 240 245 gat ttc gag gag tct ctg cgt gag cgt gtg aag gcg ttg gct ggt ttg 883 Asp Phe Giu Glu Ser Leu Arg Giu Arg Val Lys Ala Leu Ala Gly Leu 250 255 260 gat gcg tcg gtg atc gat gag gtc gct gcc gct att gag ctg acc cct 931 Asp Ala Ser Val Ile Asp Giu Val Ala Ala Ala Ile Giu Leu Thr Pro 265 270 275 ggt gcg cgc acc acg atc cgt acg ctg aac cgc atg ggt tac cag acc 979 Gly Ala Arg Thr Thr Ile Arg Thr Leu Asn Arg Met Gly Tyr Gin Thr 280 285 290 gct gtt gtt tcc ggt ggt ttc atc cag gtg ttg gaa ggt ttg gct gag 1027 Ala Val Val Ser Gly Giy Phe Ile Gin Val Leu Giu Gly Leu Ala Giu 295 300 305 gag ttg gag ttg gat tat gtc cgc gcc aac act ttg gaa atc gtt gat 1075 Giu Leu Giu Leu Asp Tyr Val Arg Ala Asn Thr Leu Giu Ile Val Asp 310 315 320 325 ggc aag ctg acc ggc aac gtc acc gga aag atc gtt gac cgc gct gcg 1123 Gly Lys Leu Thr Gly Asn Val Thr Gly Lys Ile Val Asp Arg Ala Ala 330 335 340 aag gct gag ttc ctc cgt gag ttc gct gcg gat tct ggc ctg aag atg 1171 Lys Ala Giu Phe Leu Arg Giu Phe Ala Ala Asp Ser Gly Leu Lys Met 345 350 355 tac cag act gtc gct gtc ggt gat ggc gct aat gac atc gat atg ctc 1219 Tyr Gin Thr Val Ala Val Gly Asp Gly Ala Asn Asp Ile Asp Met Leu 360 365 370 tcc gct gcg ggt ctg ggt gtt gct ttc aac gcg aag cct gcg ctg aag 1267 Ser Ala Ala Gly Leu Gly Val Ala Phe Asn Ala Lys Pro Ala Leu Lys 375 380 385 gag att gcg gat act tcc gtg aac cac cca ttc ctc gac gag gtt ttg 1315 Giu Ile Ala Asp Thr Ser Val Asn His Pro Phe Leu Asp Giu Val Leu 390 395 400 405 cac atc atg ggc att tcc cgc gac gag atc gat ctg gcg gat cag gaa 1363 His Ile Met Gly Ile Ser Arg Asp Giu Ile Asp Leu Ala Asp Gin Giu 410 415 420 WO 01/00843 WO 0100843PCT/IBOOIOO923 gac ggc act ttc cac cgc gtt cca ttg acc aat gcc taaagattcg 1409 Asp Gly Thr Phe His Arg Val Pro Leu Thr Asri Ala 425 430 tttctcgacg ccc 1422 <210> 154 <211> 433 <212> PRT <213> Corynebacterium giutarnicum <400> 154 Val Thr Glu Leu Ile Gin Asn Giu Ser Gin Giu Ile Ala Giu Leu Giu 1 Ala Thr Val1 Phe Arg Gly Pro Leu Ile 145 Leu Arg Ile Asp Gly Val1 Leu Arg Val1 Gin Arg Asp 130 Asp Lys Lys Giu Cys 210 Gin Ser Ser Gly Giu Ser Ser 115 Ile Thr Val1 Ala Arg 195 Asp Gin Gly Ala Phe Thr Vai 100 Ser Ser Ile Thr Leu 180 Ser Ser 5 Val Lys Asn Leu Val1 Val1 His Arg Arg Val 165 Aia Giy Thr Ala Asp Gin Asn 70 Thr Vali Vali Ile Gly 150 Pro Ala Leu Leu Leu Arg Vali 55 Leu Thr Giu Val1 G ly 135 Ile Asp Leu Leu Ile 215 Arg Pro 40 Gin Ala Giy Leu Val1 120 Gin Ser Val Thr Arg 200 Thr Giu 25 Gly Vai Ala Leu Gin 105 Val Thr Asp Ser Ser 185 Arg Gly 10 Gly Val Leu Phe Thr 90 Giu Leu Leu Tyr Pro 170 Glu Ser Glu Ala Tyr Thr Asp Val 75 Asp Thr Gly Ala Pro 155 Gly Leu Lys Val1 Ala 235 Leu Ala Val1 Gly Thr Val Asp Asp 140 Val1 Gly Asn Arg Ile 220 Val1 Pro Ala Giu Ile Leu Gin Pro 125 Tyr Thr Gly Val1 Leu 205 Giu Thr Ala Phe Gin Al a Lys Ser 110 Val1 Asp Gly Glu Asp 190 Val1 Met Glu Val Phe S er Pro Val Ser Asp Al a Leu Ala 175 Ile Cys Leu Arg Ile Arg Met Giu His Arg Al a Asn Glu 160 Met Ala Phe Al a Ala 240 Ala His Ala Gly Lys Giu Ala Giu Val WO 01/00843 Met Arg Gly Ala Leu Ala Ile Giu Leu 275 Met Gly Tyr 290 Giu Gly Leu 305 Leu Giu Ilie Val Asp Arg Ser Gly Leu 355 Asp Ile Asp 370 Lys Pro Ala 385 Leu Asp Giu PCTJIBOO/00923 Glu Gly 260 Thr Gin Ala Val1 Ala 340 Lys Met Leu Val Leu Asp Ile Gly Tyr 315 Asn Arg Val1 Gly Ser 395 Ser Arg Giu Arg Phe 300 Val1 Val Giu Gly Val1 380 Val Arg Giu Val1 Thr 285 Ile Arg Thr Phe Asp 365 Ala Asn Asp Leu Ala Asp Gin Giu Asp Gly Thr Phe His Arg Val Pro Leu Thr Asn 420 425 430 Ala <210> 155 <211> 490 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(490) <223> FRXA02479 <400> 155 atacatctca cccaattccc cataactaga caattgccca gcaacgactg ataagtctcc aatgtcgtgt tccgcgctca gacatgagac aattgttgcc gtg act gaa ctc atc 115 Val Thr Glu Leu Ile 1 cag aat gaa tcc caa gaa atc gct gag ctg gaa gcc ggc cag cag gtt 163 Gin Asn Glu Ser Gin Giu Ile Ala Glu Leu Giu Ala Gly Gin Gin Val 15 WO 01/00843 gca ttg cgt Ala Leu Arg gac cgc cca Asp Arg Pro cag gtt cag Gin Val Gin aac ttg gcg Asn Leu Ala acc aca ggc Thr Thr Gly gtg gag ctg Val Glu Leu gtt gtt gtg Val Val Val 120 gaa ggt Glu Gly ggt gtg Gly Val gtc ttg Val Leu gcg ttt Ala Phe ctg act Leu Thr cag gaa Gin Giu 105 gtg ttg Val Leu tat Tyr act Thr gac Asp gtg Val1 75 gac Asp act Thr gg t Gly cc t Pro gcg Ala 45 gag Giu a tc Ile ctc Leu cag Gin ccg Pro 125 gtg atc Vai Ile ttt agg Phe Arg tca atg Ser Met cct gag Pro Glu 80 gtg cat Val His 95 tcc cgt Ser Arg gat -gcg Asp Ala aca Thr gtc Val1 t tc Phe cgt Arg gga Gly cct Pro ctg Leu Ile Leu Ala Val1 Gly Thr Val1 Asp gtg Val1 t tg Leu cgt Arg gtc Val c ag Gin cgt Arg gat Asp 130 Ala Pro Ala Glu Ile Leu Gin Pro 125 PCTIBOO/00923 ggt aaa 211 Gly Lys gct aat 259 Ala Asn ttt ttg 307 Phe Leu acc gtc 355 Thr Val gtg gtg 403 Val Val 100 tcc cat 451 Ser His <210> 156 <211> 130 <212> PRT <213> Corynebacteriun giutamicum <400> 156 Val Thr Giu Leu Ile Gin Asn Giu Ser 1 Ala Gly Gin Gin Val Ala Leu Arg Giu Thr Vai Ser Gly Lys Asp Arg Pro Gly 40 Val Leu Ser Ala Asn Gin Val Gin Val 55 Phe Arg Gly Phe Leu Asn Leu Ala Ala 70 Arg Val Glu Thr Val Thr Thr Gly Leu Gly Gin Ser Val Val Val Giu Leu Gin 100 105 Pro Arg Ser Ser His Val Val Val Val 115 120 Leu Asp 130 Glu Tyr Thr Asp Val1 Asp Thr Gly WO 01/00843 WO 0100843PCT/IBOO/00923 <210> <211> <212> <213> <220> <221> <222> <223> 157 558

DNA

Corynebacterium giutamicum

CDS

(101)..(535) FRXA02758 <400> 157 aaggcgttgg ctggtttgga tgcgtcggtg atcgatgagg tcgctgccgc tattgagctg acccttggtg cgcgcaccac gatccgtacg gttgaaccgc atg ggt tac cag Met Gly Tyr Gin gct gtt gtt tcc ggt ggt ttc atc cag Ala Val Val Ser Gly Gly Phe Ile Gin ttg gaa ggt ttg Leu Giu Gly Leu gct gag Ala Glu gag ttg gag Giu Leu Giu ggc aag ctg Gly Lys Leu t tg Leu gat tat gtc cgc Asp Tyr Val Arg aac act ttg gaa atc gtt gat Asn Thr Leu Giu Ile Vai Asp acc ggc aac gtc Thr Gly Asn Val gga aag atc gtt Giy Lys Ile Val gac Asp cgc gct gcg Arg Ala Ala aag gct LYS Ala gag ttc ctc cgt Giu Phe Leu Arg ttc gct gcg gat Phe Ala Ala Asp ggc ctg aag atg Gly Leu Lys Met cag act gtc gct Gin Thr Val Ala ggt gat ggc gct Gly Asp Gly Ala gac atc gat atg Asp Ile Asp Met tcc gct gcg ggt Ser Ala Ala Gly ggt gtt gct ttc Gly Val Ala Phe gcg aag cct gcg Ala Lys Pro Ala ctg aag Leu Lys 100 gag att gcg Giu Ile Ala cac atc atg His Ilie Met 120 gat Asp 105 act tcc gtg aac Thr Ser Val Asn cca ttc ctc gac Pro Phe Leu Asp gag git ttg Giu Val Leu 115 gat cag gaa Aso Gin Giu ggc att tcc cgc Gly Ile Ser Arg gag atc gat ctg Giu Ilie Asp Leu gcg Ala 130 gac ggc Asp Gly 135 act ttc cac cgc Thr Phe His Arg cca ttg acc aat Pro Leu Thr Asn taaagattcg tttctcgacg ccc 558 <210> 158 <211> 145 <212> PRT WO 01/00843 PCT[IiBO0/00923 <213> Corynebacterium giutainicum <400> 158 Met Gly Tyr Gin Thr Ala Val Val Ser Gly Gly Phe Ile Gin Val Leu 1 5 10 Giu Gly Leu Ala Giu Giu Leu Glu Leu Asp Tyr Val Arg Ala Asn Thr 25 Leu Glu Ile Val Asp Gly Lys Leu Thr Gly Asn Vai Thr Gly Lys Ile 40 Val Asp Arg Ala Ala Lys Ala Glu Phe Leu Arg Glu Phe Ala Ala Asp 55 Ser Gly Leu Lys Met Tyr Gin Thr Val Ala Val Gly Asp Giy Ala Asn 70 75 Asp Ile Asp met Leu Ser Ala Ala Gly Leu Gly Val Ala Phe Asn Ala 90 Lys Pro Ala Leu Lys Glu Ile Ala Asp Thr Ser Val Asn His Pro Phe 100 105 110 Leu Asp Giu Val Leu His Ile Met Gly Ile Ser Arg Asp Giu Ile Asp 115 120 125 Leu Ala Asp Gin Giu Asp Gly Thr Phe His Arg Val Pro Leu Thr Asn 130 135 140 Ala 145 <210> 159 <211> 205 <212> DNA <213> Cory-nebacteriun giutamicui <220> <221> CDS <222> (72)..(182) <223> FRXA02759 <400> 159 tcttacttct aagctgaatg tgatattgcg attaagcgtt ctggtttgct gcgtcgttct aagcgtctgg tgtg ctt cga ttg tat ccc acg ttg atc act ggt gag gtc 110 Val Leu Arg Leu Tyr Pro Thr Leu Ile Thr Gly Glu Val 1 5 att gag atg ctg gcg gct cac gcg ggc aag gaa gct aaa gtt gcg gca 158 Ilie Glu Met Leu Ala Ala His Ala Gly Lys Glu Ala Lys Val Ala Ala 20 gtt act gag cgt gcg atg cgc ggg tgagctcgat ttcgaggagt ctc 205 Val Thr Giu Arg Ala Met Arg Gly <210> 160 WO 01/00843 PCTLBOO/00923 <211> 37 <212> PRT <213> Corynebacterium glutamicwun <400> 160 Val Leu Arg Leu Tyr Pro Thr Leu Ile Thr Gly Glu Val Ile Giu Met 1 5 10 Leu Ala Ala His Ala Gly Lys Glu Ala Lys Val Ala Ala Val Thr Glu 25 Arg Ala Met Arg Gly <210> 161 <211> 1188 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS <222> (101)..(1165) <223> RXA02501 <400> 161 cgccccgaaa gctttaccat ggatacgata ctcaatggag atatccatta tcggtttggc gtctgtgcgc tcatgaacca ggaaggtttt tgtttgtctc atg agc tct gaa gga Met Ser Ser Glu aga Arg C tC Leu gac Asp gaa Glu aac Asn gcc Ala gtc Val1 tcc tac Tyr t cc Ser ata Ile gcc Ala gtc Val agc Ser t tc Phe gcc gcc Ala cgc Arg aac Asn gtc Val1 gac Asp atc Ile gac Asp c aa atc Ile aat Asn gcc Ala gcg Al a gtc Val1 ga t Asp gac Asp ctc 1 acc Thr cga Arg cag Gin t ac Tyr ggC Gly ga t Asp acc Thr Cgg cca Pro cgc Arg cgc Arg ggc Gly gcc Ala gtc Val1 ctc Leu aag gat Asp ttc Phe gca Ala gag Glu gaa Glu caa Gin 100 caa Gin ttc G ly t tc Phe gaa Glu ggt Gly ctc Leu tct Ser gat Asp ggc Gly ttc WO 01/00843 Ser Ser Leu 120 acc atc aaa Thr Ilie Lys 135 ctc acc ggc Leu Thr Gly 150 gcc ctc gaa Ala Leu Giu tgc gaa gaa Cys Giu Glu acc aaa caa Thr Lys Gin 200 ctc gtc tcc Leu Vai Ser 215 ctc ggc ttc Leu Gly Phe 230 gta ttc acc Val Phe Thr aga cac gca Arg His Ala cga tgc acc Arg Cys Thr 280 atg gtc ggc 1027 Met Val Gly 295 gaa gcc gaa 1075 Glu Ala Giu 310 aaa gcc acc 1123 Lys Ala Thr agt gtc gcc 1165 Ile gaa Giu tcc Ser ttc Phe atc Ile 185 ctc Leu gca Ala acc Thr ggC Gly gtc Val1 265 gcc Al a acc Thr ac c Thr cgc Arg ggc Val1 atc Ile gaa Giu atc Ile 170 gtc Val gcc Ala acc* Thr gga Giy cga Arg 250 gca Ala tac Tyr gcc Ala cga Arg gaa Giu 330 tgg Al a ccc Pro 140 gcc Ala ggC Gly caa Gin atg Met gtc Val1 220 atc Ile gtc Val1 ctc Leu gac Asp gca Ala 300 tgg Trp gga Gly cta Gin 125 gtg Val1 gac Asp cgc Arg cgc Arg cac His 205 caa Gin Gly ggc Gly gca Ala tcc Ser 285 gta Val1 gac Asp atc Ile cgc Gly gtg Val1 gac Asp ccc Pro atg Met 190 atc Ile c tc Leu aca Thr gac Asp tcc Ser 270 atc Ile aac Asn gtc Val1 ccc Pro cgc Leu tgg Trp gtc Val1 gtc Val1 175 gcc Ala gcc Ala gcc Ala gtc Val1 atc Ilie 255 atc Ile aac Asn ccc Pro cgc Arg gcc Ala 335 cga Phe aaa Lys tcc Ser 160 caa Gin gac Asp gcc Ala caa Gin gca Ala 240 C tc Leu gaa Giu gac Asp gac Asp gat Asp 320 ctg Leu tgg Arg caa Gin 145 cgc Arg gaa Giu aaa Lys ggc Gly atc Ile 225 gaa Giu cac His caa Gin ctc Leu tcc Ser 305 ttc Phe gtc Val1 aga Lys 130 gtg Val1 Gly cta Leu atg Met cac His 210 ctg Leu gca Ala gga Gly ctc Leu ccc Pro 290 aaa Lys cga Arg ac c Thr aaa Lys aaa Lys cgc Arg gtt Val tgg Trp 195 caa Gin gca Ala aaa Lys ccc Pro gac Asp 275 atg Met c tc Leu agc Ser gcc Ala caa PCTIIBOO/00923 Phe Phe ttc aaa 547 Phe Lys gaa caa 595 Giu Gin 165 gac ctc 643 Asp Leu 180 ccc ggc 691 Pro Gly gtc tgg 739 Val Trp caa cgc 787 Gln Arg gat gga 835 Asp Gly 245 ggc aaa 883 Gly Lys 260 ctc acc 931 Leu Thr ctc tcc 979 Leu Ser cgc aaa Arg Lys atc cgc Ile Arg 325 gca ttc Ala Phe 340 Ser Val Ala Gly Trp Ser Leu Arg Arg Arg Trp Arg Lys Gin WO 01/00843 WO 0100843PCT/IBOO/00923 345 350 taacgcacag gagccgtttt aag 1188 <210> 162 <211> 355 <212> PRT <213> Corynebacteriun glutamicum <400> 162 Met Ser Ser Giu 1 Thr Pro Glu Asp Arg Arg Phe Phe Gin Arg Gin Ala Tyr Gly Met Glu Gly Ala Ile Glu Asp Val Pro Gin 100 Thr Leu Ile Gin 115 Arg Lys Lys Phe 130 Gin Val Lys Phe 145 Arg Giy Arg Glu Giu Leu Vai Asp 180 Lys Met Trp Pro 195 Gly His Gin Val 210 Ile Leu Aia Gin 225 Giu Ala Lys Asp Arg Asn His Asn Leu Asp Giu Asn 70 Aia Vali Ser Thr Leu 150 Ala Cys Thr Leu Leu 230 Vali Ser Ala Aia Phe Ala Ala Leu 120 Lys Gly Giu Glu Gin 200 Ser Phe Thr Trp, 25 Ala Al a Asn Ile Ala 105 Ile Giu Ser Phe Ile 185 Leu Aia Thr Gly Trp, 10 Ser Ala Thr Ala His Al a Val Ile Giu Ile 170 Val1 Al a Thr Gly Arg 250 Asp Ala Pro Gin Gly 75 Val1 Phe Phe Leu Asn 155 Lys Asp Asp Pro Ala 235 Leu Tyr Ser Ile Ala Val1 Ser Phe Ala Pro 140 Ala Gly Gin Met Val1 220 Ile Val Ala Gly Asp Ala Ala Pro Val1 110 Gly Val Asp Pro Met 190 I le Leu Thr Asp Ile Asn Ala Ala Val Asp Asp Leu Trp, Vali Val1 175 Ala Ala Ala Val1 Ile 255 Gly Leu Ala Ile Ala Pro Asn Phe Lys Ser 160 Gin Asp Al a Gin Al a 240 Leu His Gly Pro Gly Lys Arg His Ala Val Ala Ala Leu Aia Ser Ile Giu WO 01/00843 WO 0100843PCTIBOOIOO923 Gin Leu Ser 305 Phe Val1 Arg Asp 275 Met Leu Ser Ala Gin 355 260 Leu Leu Arg Ile Ala 340 Thr Arg Cys Thr 280 Ser Met Vai Gly 295 Lys Glu Ala Giu 310 Arg Lys Ala Thr 325 Phe Ser Val Ala Tyr Ser Asp Ser 285 Ala Val Ala Val 300 Arg Gly Trp Asp 315 Giu Tyr Gly Ile 330 Trp Ser Leu Arg 270 Ile Asn Val Pro Arg 350 <210> 163 <211> 558 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (101)..(535) <223> RXN03105 <400> 163 ggttggggtc atcaaaggat gcggacatcg ctgtggggtt aggtgccttt ctggcaggtg aatcaggact ctaagcaagc cag Gin gg t Gly gac Asp ccc Pro tac Tyr gtg Val1 att Ile agg Arg gca Ala aac Asn gac Asp gga Gly tcc Ser tta Leu gtc Val cca Pro aaa Lys gga Gly tat Tyr agc Ser tct Ser gag Giu tct Ser 75 tcc Ser ttt Phe gca Ala gaa G iu ccc Pro cca Pro gga Gly acg Thr gta Val1 30 ggt Gly cgt Arg atc Ile gcc Ala gtgtaataat tgcacctgtg ttg att ctt cca gtt Leu Ile Leu Pro Val 1 cac ctg aat cac atc His Leu Asn His Ile gaa gat ctc cgc ctc Giu Asp Leu Arg Leu acc cac gaa ctc ttc Thr His Giu Leu Phe gtc cac tat gac acc Val His Tyr Asp Thr gtt gac aat gtc att Val Asp Asn Val Ile ctc tct cca gct tat Leu Ser Pro Ala Tyr 100 115 163 211 259 307 355 403 WO 01/00843 WO 0100843PCT/IBOO/00923 ggc gaa ctc gca gca caa cga gcg gtc gga aac acc tcg ccg ctg tac 451 Gly Giu Leu Ala Ala Gin Arg Ala Val Gly Asn Thr Ser Pro Leu Tyr 105 110 115 agc gaa gac ttt cgg atc gcc tcg cat gaa cca atc aaa gag cgg tgc 499 Ser Giu Asp Phe Arg Ile Ala Ser His Giu Pro Ile Lys Glu Arg Cys 120 125 130 acg tat aga aag cta acc ttt tta agt gcg cgg ttt tagggtgaga 545 Thr Tyr Arg Lys Leu Thr Phe Leu Ser Ala Arg Phe 135 140 145 atctaacgct gag 558 <210> 164 <211> 145 <212> PRT <213> Corynebacteriun giutanicurn <400> 164 Leu Ilie Leu Pro Val Gin Glu Giy Ile Ser Tyr Phe Pro Thr Pro Leu 1 5 10 His Leu Asn His Ile Gly Gly Ser Arg Leu Ser Ala His Vai Glu Asp 25 Giu Asp Leu Arg Leu Asp Arg Asp Ala Val Ser Glu Phe Giy Arg Lys 40 Thr His Glu Leu Phe Pro Gly Vai Asn Pro Giu Pro Asn Arg Phe Ser 55 Val His Tyr Asp Thr Tyr Thr Ala Asp Lys Ser Pro Ile Ile Asp Ala 70 75 Vai Asp Asn Val Ile Val Leu Thr Gly Gly Ser Giy His Ala Phe Lys 90 Leu Ser Pro Ala Tyr Gly Giu Leu Ala Ala Gin Arg Ala Val Gly Asn 100 105 110 Thr Ser Pro Leu Tyr Ser Glu Asp Phe Arg Ile Ala Ser His Giu Pro 115 120 125 Ile Lys Giu Arg Cys Thr TIyr Arg Lys Leu Thr Phe Leu Ser Ala Arg 130 135 140 Phe 145 <210> 165 <211> 687 <212> DNA <213> Corynebacterium giutanicuxn <220> <221> CDS <222> (62)..(664) WO 01/00843 PCTIIBOO/00923 <223> RXS01130 <400> 165 agttcgtggc ggatgctgtg aacgtttccg gtggtcgcgt gggcgaagag gttctgtgtg gatg gat ctg gct cgc aag ctt ggt ctt ctt gct ggc aag ctt gtc gac 109 Met Asp Leu Ala Arg Lys Leu Gly Leu Leu Ala Gly Lys Leu Val Asp 1 5 10 gcc gcc cca gtc tcc att gag gtt gag gct cga Ala gag Glu gga Gly gaa Glu act Thr agc Ser atc Ile aac Asn ggt Gly 145 act Thr tcc Ser Ala cag Gin att Ile gag Glu cac His gca Ala acc Thr c tc Leu 130 acc Thr cag Gin gc t Ala Pro gtc Val1 atc Ile cgt Arg cgt Arg act Thr cgc Arg 115 t tc Phe aag L.ys gc t Ala gtc Val Val1 aat Asn gaa Glu ggC Gly t cc Ser gtt Val1 100 atc Ile ctg Leu c tg Leu gag Glu tct Ser 180 Ile ctt Leu tcc Ser gac Asp 70 ctg Leu gg t Gly ggc Gly tac Tyr gct Al a 150 ggt Gly gag Glu Ala gct Ala gtc Val1 aag Lys gtc Val1 gg t Gly ga t Asp cc t Pro aac Asn gtc Val1 170 gaa Arg gtt Val1 aac Asn acc Thr 75 att Ile ctt Leu ctg Leu ggt Gly atc Ile 155 c tg Leu atc ggc G ly cgt Arg gc t Ala aac Asn act Thr gag Giu cgc Arg gca Ala 140 gag Giu atc Ile aac ctt tct tcc Ser tcc Ser gct Ala gtt Val gcg Ala aag Lys ctg Leu gtt Val ttg Leu 160 gag Giu ggt Gly Giu Ile Asn Ala Giu Leu 190 taattagaga tccatttgct gct act tcc Ala Thr Ser 195 tga <210> 166 ttc cag gtt gat Phe Gin Val Asp ctt gac Leu Asp 200 WO 01/00843 PCT(1B00100923 <211> 201 <212> PRT <213> Corynebacterium giutamicun <400> 166 Met Asp Leu Ala Arg Lys Leu Gly Leu Leu Ala Gly Lys Leu Val Asp 1 5 10 Ala Ala Pro Val Ser Ile Giu Val Glu Ala Arg Gly Giu Leu Ser Ser 25 Giu Gln Val Asn Ala Leu Gly Leu Ser Ala Val Arg Gly Leu Phe Ser 40 Gly Ile Ile Giu Giu Ser Val Thr Phe Val Asn Ala Pro Arg Ile Ala 55 Glu Giu Arg Gly Leu Asp Ile Ser Val Lys Thr Asn Ser Glu Ser Val 70 75 Thr His Arg Ser Val Leu Gin Val Lys Vai Ile Thr Giy Ser Gly Ala 90 Ser Ala Thr Val Val Giy Ala Leu Thr Gly Leu Glu Arg Val Giu Lys 100 105 110 Ile Thr Arg Ile Asn Gly Arg Gly Leu Asp Leu Arg Ala Giu Gly Leu 115 120 125 Asn Leu Phe Leu Gin Tyr Thr Asp Ala Pro Gly Ala Leu Gly Thr Val 130 135 140 Gly Thr Lys Leu Gly Ala Ala Gly Ile Asn Ile Glu Ala Ala Ala Leu 145 150 155 160 Thr Gin Ala Glu Lys Gly Asp Gly Ala Val Leu Ile Leu Arg Val Glu 165 170 175 Ser Ala Val Ser Giu Glu Leu Giu Ala Giu Ile Asn Ala Giu Leu Gly 180 185 190 Ala Thr Ser Phe Gin Val Asp Leu Asp 195 200 <210> 167 <211> 604 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (101)..(604) <223> RXS03112 <400> 167 gtgagcacgc aatttctttg ctgctgtcta ctgctcgcca gatcctgctg ctgatgcgac gctgcgtgag ggcgagtgga agcggtcttc tttcaacggt gtg gaa att ttc gga 115 Val Giu Ile Phe Gly 1 WO 01/00843 WO 0100843PCT/IBOO/00923 aaa Lys cag Gin gct Ala gat Asp acc Thr aag Lys gag Giu gg t Giy aag Lys gag Giu 150 gcg Ala gtc ggt Val Gly ctt gct Leu Ala cct gct Pro Ala ctg atg Leu Met gaa act Glu Thr ggc cag Gly Gin gct ttg Ala Leu 105 gat gtg Asp Val 120 cct cag Pro Gin caa gat Gin Asp gc t Ala atc gtc ggt ttt ggc cac att Ile Val Gly Phe Gly His Ile ttt Phe gcg Ala cgt Arg ggC Giy 75 atc Ile ga t Asp tcc Ser gtt Val1 gcg Ala 155 gag Glu gct Ala tct S er atg Met a tc Ile gcg Ala acc Thr gtg Val 140 ggt Gly acc Thr cag Gln 45 gac Asp ttt Phe aac Asn att Ile gag Glu 125 act Thr act Thr ggt Gly gct Ala gag Glu att Ile ctc Leu ggt Gly cac His gat Asp ggt Giy 145 gat Asp cag ttg ttt Gin Leu Phe tac gat cct Tyr Asp Pro ttg gtt gag Leu Val Giu cac ctt cct His Leu Pro ctt gct aag Leu Ala Lys ggc ctt gtt Giy Leu Val 100 att cgt ggc Ile Arg Gly 115 tct cct ttg Ser Pro Leu 130 gct tct act Aia Ser Thr tct gtg ctc Ser Val Leu gct Ala t ac Tyr ttg Leu aag Lys t cc Ser gat Asp gct Aia ttC Phe gaa Giu aag Lys 165 163 211 259 307 355 403 451 499 547' 595 <210> 168 <211> 168 <212> PRT <2i3> Corynebacterium giutainicum <400> 168 Val Giu Ile Phe Gly Lys Thr Val Gly Ilie Val Giy Phe Gly His Ile 1 5 10 Gly Gin Leu Phe Ala Gin Arg Leu Ala Ala Phe Giu Thr Thr Ile Val 25 Ala Tyr Asp Pro Tyr Ala Asn Pro Ala Arg Ala Ala Gin Leu Asn Val 40 WO 01/00843 Glu Leu Vai Ile His Leu Leu Leu Ala Gly Gly Leu His Ile Arg 115 Asp Ser Pro 130 Gly Ala Ser 145 Asp Ser Val Glu Pro Lys Val1 100 G ly Leu Thr Leu Leu Lys Ser Asp Ala Phe Glu Lys 165 Asp Thr 70 Lys Giu Gly Lys Glu 150 Ala Glu 55 Lys Lys Gin Phe Leu 135 Ala Leu Leu Glu Gly Al a Asp 120 Pro Gin Ala Arg Gly 75 I le Asp Ser Val1 Ala 155 Ser met Ile Al a Thr Val1 140 Gly PCT/iBOO/00923 Val Thr Ala His Ala Arg Ser Gly Cys Thr His Leu Ile Ala 160 <210> 169 <211> 1458 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (101)..(1435) <223> RXN00969 <400> 169 ctatagtggc taggtaccct ttttgttttg gacacatgta taggacaaca acgctcgacc gcgattattt ttggagaatc gcc Ala gcc Ala acc Thr gtt Val1 gca Ala cca Pro ctt Leu gag Glu cg t Arg cc t Pro agc ttt Ser *Phe tta gga Leu Gly tac ggt Tyr Gly ggc att Gly Ile gag ctg Glu Leu aac Asn ttc Phe gat Asp gct Ala ctc Leu ccc Pro gga Gly gaa Giu gtt Val act Thr 75 ggc Gly aca Thr ctt Leu tc t Ser 60 gag Glu ggc Gly gag Glu att Ile aag Ly s gca Ala 80 gggtggccga aacaaagtaa atg acc tca gca tct Met Thr Ser Ala Ser 1 tca gca gtc gga att Ser Ala Val Gly Ile gtg atg cgt ctg atg Val Met Arg Leu Met ggt ggc cca ctg gag Gly Gly Pro Leu Glu cca cgt gaa ggc gtt Pro Arg Glu Gly Val ctc atc gag cgc gag Leu Ile Glu Arg Glu 115 163 211 259 307 355 WO 01/00843 WO 0100843PCT/EBOO/00923 gat gtt Asp Val gag gta Glu Val aat aag Asn Lys gaa gcc Glu Ala 135 att cca Ilie Pro 150 cag tct Gin Ser gcc atg Ala Met act cgt Thr Arg cat gac His Asp 215 acc cgt Thr Arg 230 aac gct Asn Ala ttg ttg Leu Leu att tct Ilie Ser gcg tcg 1027 Ala Ser 295 gct ggt 1075 gac Asp gt t Val gc t Al a 120 gca Al a gtg Val1 gtg Val1 ga t Asp ttg Leu 200 gcc Al a gtt Val gcc Ala gcc Ala gct Al a 280 gta Val cgc atc Ile ctc Leu 105 ctt Leu aac Asn gt t Val1 atg Met tcc Ser 185 ggt Gly gca Al a acc Thr gac Asp atc Ile 265 cgc Arg aac Asn ctg gt t Val1 gct Ala gca Ala gac Asp cca Pro 155 atc Ile ggc Gly gcc Ala aag Lys gat Asp 235 gag Giu gag Giu cac His tcc Ser ttc gag Glu ctg Leu gct Ala ctg Leu 140 ctg Leu gtt Val1 gct Ala gaa Glu gc t Al a 220 gat Asp gca Ala aag Lys ccg Pro tt t Phe 300 tac atc Ile gcc Ala 110 tct Ser t tc Phe cgc Arg ggc Gly tat Tyr 190 gat Asp at t Ile tac Tyr cag Gin acc Thr 270 c ta Leu gcai Ala aac ggc Gly ggc Gly gct Ala gag Glu tcc Ser acc Thr 175 gca Ala cca Pro ttg Leu tgc Cys cag Gin 255 aac Asn tta Leu atc Ile ggt ggc Gly aag Lys gag Glu gc t Ala c tg Leu 160 acc Thr ga t Asp act Thr gca Ala gaa Glu 240 gc a Ala aag Lys cct Pro ttt Phe gca att Ile tc t Ser ctt Leu gc t Al a 145 gc t Ala aac Asn tc t Ser gc a Ala tgc Cys 225 ggt Gly 9gc Giy gaa Glu gtg Val gtt Val1 305 ggt gag Glu gtt Val gc t Ala 130 gt t Val1 ggc Gly ttc Phe ttg Leu aac Asn 210 atc Ile att Ile cac His gga Gly tcc Ser 290 gaa Glu 99C tac Tyr gtt Val1 115 gat Asp gca Ala gat Asp atc Ile gc t Ala 195 gtc Val gc t Ala agg Arg acc Thr aag Lys 275 cac His gca Ala gcg cca Pro 100 acc Thr gca Ala tgc Cys c ag Gin t tg Leu 180 gag Glu gaa Glu t tc Phe aac Asn atc Ile 260 tcg Ser cca Pro gaa Glu cca cg t Arg gcc Ala gcg Ala gca Ala atc Ile 165 gac Asp gca Ala ggc Gly cac His atc Ile 245 aag Lys gc t Ala ctg Leu gca Ala acc Ala Gly Arg Leu Met Phe Tyr Gly Asn Gly Ala Gly Gly Ala Pro Thr WO 01/00843 310 gcg tct gct 1123 Ala Ser Ala cac ggt ggc 1171 His Gly Gly gct gat ttc 1219 Ala Asp Phe 360 gaa gat cgc 1267 Giu Asp Arg 375 caa gga atc 1315 Gin Gly Ilie 390 gca cgt ctg 1363 Ala Arg Leu cgc acc gtt 1411 Arg Thr Val agt gtg atc 1458 Ser Val Ile 440 gtg Val1 cgt Arg 345 gg t Gly gtg Val1 tcc Ser atc Ile gaa Giu 425 cgc Arg ctt.

Leu 330 gct Ala gag Giu ggg Gly c tg Leu gtg Val1 410 ctg Leu ctc Leu 315 ggc Gly cca Pro acc Thr gtt Val1 cgt Arg 395 gtc Val1 ctg Leu gaa Glu gtc gtt Val Val gag tcc Glu Ser 350 act cgt Thr Arg 365 gct gaa Ala Glu atc cga Ile Arg cac tct His Ser gct aag Ala Lys 430 cga Arg aac Asn gac Asp 370 c tg Leu cgc Arg tct Ser aag.

PCTIBOO/00923 325 ag gtg ys Val cg atc ro Ile at gtg sp Val ct gag er Glu at gat sp Asp 405 tt tCC eu Ser tc aac Pro Val Val Lys Ala Ile Asn 435 gac Asp 445 <210> 170 <211> 445 <212> PRT <213> Corynebacterium glutamicun <400> 170 Met Thr Ser Ala Ser Ala Pro Ser 1 5 Ser Ala Val Gly Ile Ala Leu Leu Val Met Arg Leu Met Thr Glu Tyr 40 Gly Gly Pro Leu Glu Val Arg Gly 55 taattttact gacatggcaa ttg Phe Asn Pro Gly Lys Gly Pro Gly 10 Gly Phe Gly Thr Val Gly Thr Glu 25 Gly Asp Glu Leu Ala His Arg Ile Ile Ala Val Ser Asp Ile Ser Lys WO 01/00843 WO 0100843PCTIBOO/00923 Pro Leu Ile Ser Leu Ala 145 Ala Asn Ser Ala Cys 225 Gly Gly Glu Val1 Val1 305 Gly Ala Ala Leu Ser Arg Ile Glu Val1 Ala 130 Val Gly Phe Leu Asn 210 Ile Ile His Gly Ser 290 Glu Gly Arg Asn Asp 370 Leu Glu Giu Tyr Val 115 Asp Ala Asp Ile Ala 195 Val Ala Arg Thr Lys 275 His Ala Ala Asn Leu 355 Met Phe Gly Arg Pro 100 Thr Ala Cys Gin Leu 180 Glu Giu Phe Asn Ile 260 Ser Pro Giu Pro Lys 340 Pro Asp Ser Val1 Glu Arg Ala Ala Ala Ile 165 Asp Ala Gly His Ile 245 Lys Ala Leu Ala Thr 325 Val1 Ile Val Glu Pro Val1 Val1 Lys Ala 135 Pro Ser Met Arg Asp 215 Arg Ala Leu Ser Ser 295 Gly Ser Gly Asp Asp 375 Gly Glu Asp Val Ala 120 Ala Val Val Asp Leu 200 Ala Val Ala Ala Ala 280 Val Arg Ala Gly Phe 360 Arg Ile Leu Ile Leu 105 Leu Asn Val Met Ser 185 Gly Al a Thr Asp Ile 265 Arg Asn Leu Val1 Arg 345 Gly Val Ser Leu Val1 Ala Val1 Val Gly Gly 170 Thr Tyr Ser Ala Ile 250 Cys Val Lys Met Leu 330 Ala Giu Gly Leu Thr 75 Val1 Ala Ala Asp Pro 155 Ile Gly Ala Lys Asp 235 Glu Giu His Ser Phe 315 Gly Pro Thr Val1 Arg Giu Giu Leu Ala Leu 140 Leu Val1 Ala Glu Ala 220 Asp Ala Lys Pro Phe 300 Tyr Asp Gly Thr Leu 380 Thr Asp Val Lys His 125 Tyr Arg Asn Asp Ala 205 Ala Val Ala Phe Thr 285 Asn Gly Val1 Glu Thr 365 Ala Ile Ala Ile Ala 110 Ser Phe Arg Gly Tyr 190 Asp Ile Tyr Gin Thr 270 Leu Ala Asn Val1 Ser 350 Arg Giu Arg Ala G ly Lys Giu Ala Leu 160 Thr Asp Thr Ala Giu 240 Ala Lys Pro Phe Ala 320 Ala Tyr His Ala Glu WO 01/00843 PCTIIB0O/00923 385 390 395 400 Glu Arg Asp Asp Asp Ala Arg Leu Ile Val Val Thr His Ser Ala Leu 405 410 415 Glu Ser Asp Leu Ser Arg Thr Val Glu Leu Leu Lys Ala Lys Pro Val 420 425 430 Val Lys Ala Ile Asn Ser Val Ile Arg Leu Glu Arg Asp 435 440 445 <210> 171 <211> 493 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (493) <223> FRXA00974 <400> 171 ctatagtggc taggtaccct ttttgttttg gacacatgta gggtggccga aacaaagtaa taggacaaca acgctcgacc gcgattattt ttggagaatc atg acc tca gca tct Met Thr Ser Ala Ser gcc Ala gcc Ala acc Thr gtt Val1 gca Ala gat Asp gag Glu cca Pro ctt Leu gag Glu cgt Arg cc t Pro gt t Val1 gta Val agc Ser tta Leu tac Tyr ggc Gly gag Glu gac Asp gt t Val ggc aag Gly Lys aca gtc Thr Val ctt gcg Leu Ala 45 tct gat Ser Asp gag gac Glu Asp gag gtt Glu Val ctg aag Leu Lys gg t Gly ggc Gly cac His atc Ile gct Ala atc Ile gcc Ala 110 ccc Pro act Thr cgc Arg tca Ser ttt Phe ggc Gly ggc Gly gc t Ala ggc Gly gag Glu att Ile aag Lys gca Ala 80 9gc Gly aag Lys gag Glu gca Ala atg Met ggc Gly cgt Arg atc Ile gag Glu gtt Val gc t Ala 130 gga Gly ctg Leu c tg Leu 99c Gly cgc Arg cca Pro 100 acc Thr att Ile atg Met gag Glu gtt Val gag Glu cgt Arg gcc Ala 115 163 211 259 307 355 403 451 aat aag gct ctt gtt gca gct cac tct Asn Lys Ala Leu Val Ala Ala His Ser 120 125 WO 01/00843 WO 0100843PCT/IBOO/00923 <210> 172 <211> 131 <212> PRT <213> Corynebacteriun glutamicum <400> 172 Met Thr Ser Ala Ser Ala Pro Ser Phe Asn Pro 1 5 Ser Ala Val Gly Ile Ala Leu Leu Gly Phe Gly 25 Val Met Arg Leu Met Thr Glu Tyr Gly Asp Glu 40 Gly Gly Pro Leu Glu Val Arg Gly Ile Ala Val Pro Arg Glu Gly Val Ala Pro Glu Leu Leu Thr 70 Leu Ile Glu Arg Glu Asp Val Asp Ile Val Val Ilie Glu Tyr Pro Arg Glu Val Val Leu Ala Ala 100 105 Ser Val Val Thr Ala Asn- Lys Ala Leu Val Ala 115 120 Leu Ala Asp 130 <210> 173 <211> 1050 <212> DNA <213> Corynebacterium glutamicun <2 <221> CDS <222> (101)..(1027) <223> RXA00970 <400> 173 gaatctgatc tttcccgcac cgttgaactg ctgaaggcta aacagtgtga tccgcctcga aagggactaa ttttactgac aac gtc ggt cgt aag gtt acc gtc acg gta cct Asn Val Gly Arg Lys Val Thr Val Thr Val Pro 15 ctc gga cct ggc ttt gac act tta ggt ttg gca Leu Gly Pro Gly Phe Asp Thr Leu Gly Leu Ala 30 act gtc gaa gtg gaa att att cca tct ggc ttg 251 Lys Val Ala Asp Asp Val1 Lys His 125 Gly Gly His Ile Ala Ile Ala 110 Ser agcctgttgt taaggcaatc atg gca att gaa ctg 115 Met Ala Ile Glu Leu 1 gga tct tct gca aac 163 Gly Ser Ser Ala Asn ctg tcg gta tac gac 211 Leu Ser Val Tyr Asp gaa gtg gaa gtt ttt 259 WO 01/00843 Thr Vai Giu ggc gaa ggc Gly Giu Gly aaa gct att Lys Ala Ile ttg cga gtg Leu Arg Val tcc tct gct Ser Ser Ala gcg gat ttc Ala Asp Phe 120 ttt gaa ggc Phe Glu Gly 135 gtg gtg tcg Vai Vai Ser 150 tat gct gct Tyr Ala Ala ctg gtt cct Leu Val Pro ccc act gaa Pro Thr Glu 200 gca gtg atg Ala Val Met 215 ggt act cgt Giy Thr Arg 230 att acc tct Ilie Thr Ser tac ctt tcc Tyr Leu Ser att cca gac Val Gu Ile Ile Pro Ser Gly Leu Giu 45 caa Gin cgt Arg gtg Val1 gca Aia 105 ccg Pro cac His tgg Trp gta Vai aat Asn 185 gtc Vali atc Ile gac Asp gag Giu ggt Giy 265 aag ggc Gly gc t Aia tgc Cys gcg Aia ctg Leu cca Pro aca Thr cca Pro 170 ttc Phe act Thr gtt Val cgt Arg tgg Trp 250 gcc Aia gtt gaa gtc Giu Vai 60 ggc ctg Gly Leu 75 cac aac His Asn gcg gtt Ala Val act caa Thr Gin gat aat Asp Asn 140 aat ctg Asn Leu 155 ctt gag Leu Giu cac gca His Ala cac atc His Ilie gcg ttg Ala Leu 220 ctg cac Leu His 235 gta aac Val Asn ggc cca Giy Pro ttg gaa cc t Pro aag Lys aac Asn gc t Ala gag Glu 125 gc t Ala tc t Se r gtg Val1 tcc Ser gat Asp 205 cag Gin cag Gin cgc Arg acc Thr ga t ctt Leu gca Ala att Ile gg t Gly 110 cag Gin gcg Ala atc Ile cag Gin ac c Thr 190 gcg Ala cag Gin cct Pro ctg Leu gcc Ala 270 gct gat

ASP

gc t Ala ccg Pro 95 gtt Val1 att Ile gc t Ala gac Asp gac Asp 175 gaa Giu cga Arg cgt Arg tat Tyr cgc Arg 255 atg Met cgt ggc Gly gac Asp 80 cag Gin gct Ala gt t Val1 tc t Ser ggc Gly 160 aa t Asn gc t Ala ttt Phe cc t Pro cgt Arg 240 aac Asn gtg Val1 gag tcc Ser gct Ala tc t Ser gca Ala cag Gin gtg Val 145 aag Lys att Ile gtg Val aac Asn gat Asp 225 gca Ala cgt Arg ctg Leu tc t PCTJIBOO/00923 Val Giu Val Phe cac ctg gtg gtt 307 His Leu Val Val gaa gtt cct gga 355 Giu Val Pro Gly cgt ggt ctt ggc 403 Arg Gly Leu Gly 100 gct aat ggt ttg 451 Ala Asn Gly Leu 115 ttg tcc tct gcc 499 Leu Ser Ser Ala 130 ctg ggt gga gca 547 Leu Gly Gly Ala agc cag cca cag 595 Ser Gin Pro Gin 165 cgt gcg act gcg 643 Arg, Ala Thr Ala 180 cgc cga gtc ctt 691 Arg Arg Val Leu 195 gtg tcc cgc gtt 739 Val Ser Arg Val 210 ttg ctg tgg gag 787 Leu Leu Trp, Giu gaa gtg ttg cct 835 Glu Val Leu Pro 245 ggc tac gcg gca 883 Gly Tyr Ala Ala 260 tcc act gag cca 931 Ser Thr Giu Pro 275 ggc att aag gtg 979 Ile Pro Asp Lys Val Leu Giu Asp Ala Arg Giu Ser Gly Ile Lys Val WO 01/00843 WO 0100843PCTTBOO/00923 280 285 290 ctt gag ctt gag gtt gcg gga cca gtc aag gtt gaa gtt aac caa cct 1027 Leu Giu Leu Glu Val Ala Gly Pro Val Lys Val Glu Val Asn Gin Pro 295 300 305 taggcccaac aaggaaggcc ccc 1050 <210> 174 <211> 309 <212> PRT <213> Corynebacterium glutanicum <400> 174 Met Ala Ile Giu Leu Asn Val Gly Arg 1 Gly Leu Giu Ser Ala Ser Ala Gin Vai 145 Lys Ile Val Asn Leu Thr Giy Lys 70 Leu Ser Ala Phe Vali 150 Tyr Leu Pro Ala Gly Val1 Giu Ala Arg Ser Asp Giu 135 Val1 Ala Val1 Thr Val1 215 Gly Val1 Gin Arg Val Ala 105 Pro His Trp Val Asn 185 Val Ile Lys 10 Phe Glu Gly Ala Cys Ala Leu Pro Thr Pro 170 Phe Thr Val1 Arg Val Asp Ile Giu Gly 75 His Ala Thr Asp Asn 155 Leu His His Ala Leu Thr Thr Ile Val Leu Asn Val.

Gin Asn 140 Leu Giu Ala Ile Leu 220 His Vali Leu Pro Pro Lys Asn Ala Glu 125 Aia Ser Val1 Ser Asp 205 Gin Gin Thr Gly S er Leu Ala Ile Gly 110 Gin Ala Ile Gin Thr 190 Ala Gin Pro Val1 Leu Giy Asp Aia Pro Val Ile Ala Asp Asp 175 Giu Arg Arg Tyr Pro Ala Leu Gly Asp Gin Ala Val Ser Gly 160 Asn Ala Phe Pro Arg 240 Asp Leu Leu Trp Giu Gly Thr Arg Asp 225 230 WO 01/00843 WO 0100843PCT/I1BOO/00923 Ala Giu Val Leu Pro Ile Thr Ser Giu Trp Val Asn Arg Leu Arg Asn 245 250 255 Arg Gly Tyr Ala Ala Tyr Leu Ser Gly Ala Gly Pro Thr Ala Met Val 260 265 270 Leu Ser Thr Giu Pro Ile Pro Asp Lys Val Leu Glu Asp Ala Arg Glu 275 280 285 Ser Gly Ile Lys Val Leu Giu Leu Giu Val Ala Gly Pro Val Lys Val 290 295 300 Glu Val Asn Gin Pro 305 <210> 175 <211> 1566 <212> DNA <213> Corynebacteriun giutanicun <22 0> <221> ODS <222> (101)..(1543) <223> RXA00330 <400> 175 gcaacacttt agggtatcgc gtgggcgaag tcaccttttt caacatattt gagacggtgt gggggagtat tgtgtcaccc cttgggatag ggttatatcc acq Thr ggc Gly caa Gin gaa Glu gac Asp tac Tyr gac Asp agc Ser cca Pro gcc Ala gct Ala gaa Giu aac Asn acc Thr ggC Gly ctg Leu gct Ala atc Ile gaa Giu gcc Al a ctg Leu aaa Lys gaa Glu gcg Ala atc Ilie tcc cgc Arg 15 tac tgg Trp gt t Val atc Ile gtt Val1 95 gaa Glu ttc Phe ctg Leu cgt Arg atc Ilie acc Thr 80 cc t Pro ggc Gly gtg gac Val Asp 1 agt gat Ser Asp cct gca Pro Ala gag gta Giu Val tcc ctg Ser Leu gca cgc Ala Arg gtc acc Val Thr cca acc Pro Thr tac att tcg Tyr Ile Ser att ttg ctg Ile Leu Leu acc tac cct Thr Tyr Pro tta gcc aac Leu Ala Asn ttt gtt gat Phe Val Asp gcc tac-acc Ala Tyr Thr gaa ctc gag Giu Leu Glu 100 gct gca ttc Ala Ala Phe aac att tac ctg ggc cac ctt Asn Ile Tyr Leu Gly His Leu Ser WO 01/00843 WO 0100843PCT/IBOO/00923 aaa gac Lys Asp aig gcc atg cag cig ctc ggc gaa ctt ttc Met Ala Met Gin Leu Leu Gly Giu Leu Phe 120 cgc cgc cgc Arg Arg Arg 135 acc ggc icc Thr Gly Ser 150 gta ttc aig Val Phe Met cag atg ttt Gin Met Phe ggc gtt ttc Gly Val Phe 200 gca gaa ttc Ala Giu Phe 215 tgg gca cgc Trp Ala Arg 230 cgc acc aca Arg Thr Thr ggc aac tic Gly Asn Phe ctt ccc atc Leu Pro Ilie 280 gac gag tic 1027 Asp Giu Phe 295 acc cac gag 1075 Thr His Giu 310 tic gag cgt 1123 gaa Giu gcg Ala ac c Thr 170 cii Leu gati Asp aaa Lys atg met agc Ser 250 gac Asp cgc Arg cgt Arg icc Ser aic acc Thr gaa Giu 155 cca Pro gac Asp tgc Cys gac Asp gca Ala 235 aai Asn ati Ile cic Leu acc Thr tca Ser 315 iic atc Ile 140 iac Tyr gc t Ala gat Asp caa Gin aac Asn 220 cag Gin gac Asp i gc Cys aic Ile Gly 300 cci Pro gac 125 aac Asn gcc Ala ggc Gly cca Pro gac Asp 205 cgc Arg gt i Val1 caa Gin gca Ala gig Val 285 gac Asp icg Ser c ig gaa iac gag Glu Tyr Giu 130 acc ici ggc Thr Ser Gly gag gga aic Giu Gly Ile tic cag caa Phe Gin Gin 180 aic gcc cic Ile Ala Leu 195 gic icc gcc Val Ser Ala 210 aac icc aic Asn Ser Ile icc ica igg Ser Ser Trp icc gia cca Ser Val Pro 260 cgc caa aig Arg Gin Met 275 aac gat gig Asn Asp Vai 290 agc icc gca Ser Ser Ala cgc gcc icc Arg Ala Ser gcc acc cgc cii Leu ga t Asp cgc Arg 165 gca Ala gac Asp gac Asp aac Asn aic Ile 245 acc Thr gga Gly cic Leu gac Asp aac Asn 325 gic 499 547 595 643 691 739 787 835 883 931 979 cgc gac Phe Giu Arg Phe Ile Phe Asp Leu Leu Gly Arg Asp Ala Thr Arg Vai 330 335 340 WO 01/00843 PCTIB0O/00923 aac gat cta ttt ggt acc cag gtt cgc caa ggc gga ttc tca ctg gct 1171 Asn Asp gat gac 1219 Asp Asp gga cga 1267 Gly Arg 375 tcc cgc 1315 Ser Arg 390 gtg gca 1363 Val Ala gaa act 1411 Glu Thr ggt gaa 1459 Gly Glu cca ttc 1507 Pro Phe 455 Leu Phe 345 gcc aac Ala Asn Gly Thr Gin Val ttt gag aag gct Phe Giu Lys Ala 365 cat gct gac cgt His Ala Asp Arg Gin Gly Gly Phe gca gaa tac ggt Ala Giu Tyr Gly 370 Ser Leu Ala 355 ttc gcc tcc Phe Ala Ser 360 tcc Ser acc Thr 380 gtg gca acc atc gct gac gtg cat Val Ala Thr Ile Ala Asp Val His 385 ccc cac acc gcc gac ggc gtt cac Pro His Thr Ala Asp Gly Val His ctc gac gta Leu Asp Val cgc cag tgg Arg Gin Trp 410 gca ctc cca Ala Leu Pro cta Leu 395 atc Ile gat Asp 400 agg gac gag gtc Arg Asp Glu Val gtg aaa ttt gcc Val Lys Phe Ala 430 act cca gag cgt Thr Pro Glu Arg aac Asn 415 acc Thr cca atc atc gtc cta Pro Ile Ile Val Leu 420 425 gca cct Ala Pro caa Gin gac acc atc gtc gaa gca att Asp Thr Ile Val Giu Ala Ile 435 ttc gcc gcg atc atg gat gct Phe Ala Ala Ile Met Asp Ala 450 gac acc gat gca gtt aag cag Asp Thr Asp Ala Val Lys Gin 440 445 aag gtt tcc gac cta Lys Val Ser Asp Leu 460 gtc gat gcg att gca cca aac Pro Asn 465 aac act tcc gtg aag tac ata taacttgctt Tyr Ile Val Asp 470 tacgccaagg cct 1566 Ala Ile Ala Asn Thr Ser Val Lys 475 480 <210> 176 <211> 481 <212> PRT <213> Corynebacterium giutamicum <400> 176 Val Asp Tyr Ile Ser Thr Arg Asp Ala Ser Arg Thr Pro Ala Arg Phe 1 5 10 Ser Asp Ile Leu Leu Gly Gly Leu Ala Pro Asp Gly Gly Leu Tyr Leu 25 WO 01/00843 Pro Ala Th 3 Giu Val Le Ser Leu P1 Ala Arg Al Val Thr G] Pro Thr Al 11 Phe Giu T 130 Ala Thr SE 145 Arg Giu Gl Pro Phe G) Asn Ilie Al Ala Vai SE 210 Val Asn SE 225 Val Ser SE Phe Ser Vz Ala Arg G1 Glu Asn Af 290 Arg Ser SE 305 Ser Arg Al Asp Ala T1 PCTIIBOOIOO923 r 5 u e a u a 5 r r y n a r r r 1 n

P

r a r Tyr Ala Val1 Tyr Leu 100 Ala Glu Gly Ile Gin 180 Leu Ala Ile Trp Pro 260 Met Val Ala Ser Arg 340 Pro Asn Asp Thr Giu Phe Leu Asp Arg 165 Ala Asp Asp Asn Ile 245 Thr Gly Leu Asp Asn 325 Val Ser Ala Lys Asp Leu Leu 125 Asn Ala Gly Pro Asp 205 Arg Val Gin Ala Val 285 Asp Ser Leu Val1 Lys Glu Ala Ile Ser 110 G iy Ile Met Arg Asn 190 Val1 Ile Val1

LYS

Gly 270 Ala Tyr Met Leu Arg 350 Arg Ile Thr Pro Gly Leu Gly Gly 160 Thr Phe Lys Ala Tyr 240 Ser Ile Asn Val1 Ile 320 Arg Gly Gly Phe Ser Leu Ala Asp Asp Ala Asn Phe Giu Lys Ala Ala Ala Giu WO 01/00843 355 Tyr Gly Phe 370 Ile Ala Asp 385 Ala Asp Gly Pro Ile Ilie Ile Val Giu 435 Ala Ile Met 450 Asp Ala Val 465 Lys PCT/IBOO/00923 Ser His His 405 Leu Ile Ala Gin Gly Ser 390 Vai Giu Gly Pro Tyr 470 Asp 380 Ile

ASP

Lys Pro Leu 460 Ala 365 Arg Asp Giu Phe Giu 445 Pro Asn Thr Thr 400 Thr Thr Ala Thr Val1 480 <210> 177 <211> 1254 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (101)..(1231) <223> RXN00403 <400> 177 tttttcagac tcgtgagaat gcaaactaga ctagacagag aagttttagt cttgtccacc cagaacaggc ggttattttc cc t Pro gcc Ala gaa Giu cac His ctc gaa G iu aca Thr aaa Lys gat Asp gcc gcg Ala gaa Glu cgc Arg gca Ala act 258 ctgtccatat acactggacg atg ccc acc ctc gcg Met Pro Thr Leu Ala 1 gat gtc tcc acc gaa Asp Val Ser Thr Glu tat cac cgc tgg ggt Tyr His Arg Trp Gly gtc gtt ctc atc gaa Val Val Leu Ile Giu tgg tgg gct gac ttg Trp, Trp Ala Asp Leu tac tgc gtg atc tgt WO 01/00843 Leu Gly Pro acc aac gtc Thr Asn Val cat cca gat His Pro Asp cgt gat cag Arg Asp Gin 120 acc acg gtc Thr Thr Val 135 cta gag tgg Leu Glu Trp 150 ctt gca gtt Leu Ala Val gcc caa att Ala Gin Ile tac tac gaa Tyr Tyr Glu 200 cgc atc gcc Arg Ilie Ala 215 ttc ggc acc Phe Gly Thr 230 aag ccc gac Lys Pro Asp gac aag cta Asp Lys Leu gac gcc ctc Asp Ala Leu 280 aag gca ctc 1027 Gly Lys Ala Ile Asn Thr Asp Ile Tyr Cys Val 75 80 atc Ile gga Gly 105 gta Val gcc Ala gcc Ala tct Ser aag Lys 185 tcc Ser cac His aaa Lys cag Gin gta Val1 265 aac Asn gaa gg t Gly aa t Asn aac Asn gca Ala gca Ala gca Ala i7 0 gcg Ala ggc Gly ctc Leu gcc Ala cgc Arg 250 cag Gin cgc Arg tcC ggt Gly t tc Phe gcc Ala gta Val1 atg Met 155 cgc Arg att Ile tgc Cys acc Thr caa Gin 235 ttc Phe cgt Arg c ac His atc cct Pro gcc Ala gca Ala 130 gg t Gly gca Aia ggc Gly cac His ggC Gly 210 atc Ile ggt Gly gac Asp gtc Val gga Gly 290 gca ggc Gly acg Thr 115 ctc Leu gcc Ala gc t Al a at t Ile gaa Glu 195 gcc Ala gac Asp ccc Pro tac Tyr ttg Leu 275 ggc Gly ggc PCTIBOO/00923 Ile Cys tcc atg 403 Ser Met 100 tcc att 451 Ser Ile ggc atc 499 Gly Ile cgc acc 547 Arg Thr gct gtt 595 Ala Val 165 caa tcc 643 Gln Ser 180 ggc aac 691 Gly Asn gcc cga 739 Ala Arg gaa cgc 787 Giu Arg tac cgc 835 Tyr Arg 245 caa gca 883 Gln Ala 260 ctc acc 931 Leu Thr ctc aac 979 Leu Asn gta gat Lys Ala Leu Glu Ser Ilie Lys Val Pro Val Leu Al GyVa As Ala Gly Val Asp WO 01/00843 acc gat att ttg tac ccc tac cac cag caa gaa 1075 Thr Asp Ile Leu Tyr Pro Tyr His Gin Gin Giu 310 315 320 ctg gga aat cta ctg gca atg gca aaa atc gta 1123 Leu Gly Asn Leu Leu Ala Met Ala Lys Ile Val 330 335 gat gct ttc ctc acc gaa agc cgc caa atg gat 1171 Asp Ala Phe Leu Thr Giu Ser Arg Gin Met Asp 345 350 ttc ttc agc ctc atc tcc cca gac gaa gac aac 1219 Phe Phe Ser Leu Ile Ser Pro Asp Giu Asp Asn 360 365 gag ttc tac atc taataggtat ttacgacaaa tag 1254 Giu Phe Tyr Ile 375 <210> 178 <211> 377 <212> PRT <213> Corynebacterium giutamicun <400> 178 Met Pro Thr Leu Ala Pro Ser Gly Gin Leu Giu ctc Leu cc t Pro atc Ile tcg Ser 370 tcc Ser gtc Val gtg Val1 355 acc Thr PCTIiBOO/00923 aga aac Arg Asn 325 ggc cac Gly His 340 agg aac Arg Asn tac atc Tyr Ile 1 Asp Tyr Val1 Trp Tyr Gly Pro Asp Gly 145 Thr Trp Ile Asp Ile Ser 100 Ser Gly Arg Ala Giu His Leu 70 Thr His Arg Thr Leu 150 Ile 25 Val1 Thr Gly Ile Gly 105 Val1 Ala Ala Gin Ala Gly Asn Asn Asn Gly Lys 125 Gly Pro Ala Giu Arg Ala Thr Gly Asn 110 Gin Gly Glu Gly Ala Asn Asp Ile Thr Phe Leu Met Val WO 01/00843 WO 0100843PCT/IB00100923 Gly Ala Ala Ala Val Leu Ala Val Ser Ala Arg Ala Ser Ala Trp, Gin 165 170 175 Ilie Gly Ilie Gin Ser Ala Gin Ilie Lys Ala Ile Giu Asn Asp His His 180 185 190 Trp His Giu Gly Asn Tyr Tyr Giu Ser Gly Cys Asn Pro Ala Thr Gly 195 200 205 Leu Gly Ala Ala Arg Arg Ile Ala His Leu Thr Tyr Arg Gly Glu Leu 210 215 220 Glu Ilie Asp Giu Arg Phe Giy Thr Lys Ala Gin Lys Asn Giu Asn Pro 225 230 235 240 Leu Gly Pro Tyr Arg Lys Pro Asp Gin Arg Phe Ala Val Glu Ser Tyr 245 250 255 Leu Asp Tyr Gin Ala Asp Lys Leu Val Gin Arg Phe Asp Ala Gly Ser 260 265 270 Tyr Val Leu Leu Thr Asp Ala Leu Asn Arg His Asp Ie Gly Arg Asp 275 280 285 Arg Gly Gly Leu Asn Lys Ala Leu Glu Ser Ile Lys Val Pro Val Leu 290 295 300 Val Ala Gly Val Asp Thr Asp Ile Leu Tyr Pro Tyr His Gin Gin Giu 305 310 315 320 His Leu Ser Arg Asn Leu Gly Asn Leu Leu Ala Met Ala Lys Ile Val 325 330 335 Ser Pro Val Gly His Asp Ala Phe Leu Thr Giu Ser Arg Gin Met Asp 340 345 350 Arg Ile Val Arg Asn Phe Phe Ser Leu Ile Ser Pro Asp Giu Asp Asn 355 360 365 Pro Ser Thr Tyr Ile Glu Phe Tyr Ile 370 375 <210> 179 <211> 1210 <212> DNA <213> Corynebacterium giutarnicum <220> <221> CDS <222> (101)..(1210) <223> FRXA00403 <400> 179 tttttcagac tcgtgagaat gcaaactaga ctagacagag ctgtccatat acactggacg aagttttagt cttgtccacc cagaacaggc ggttattttc atg ccc acc ctc gcg 115 Met Pro Thr Leu Ala 1 WO 01/00843 cet tea ggt Pro Ser Gly gee gga gca Ala Gly Ala gaa tac ege Giu Tyr Arg eae gee ete His Aia-Leu etc ggt ec Leu Gly Pro ace aac gte Thr Asn Val cat eca gat His Pro Asp cgt gat eag Arg Asp Gin 120 ace aeg gte Thr Thr Val 135 eta gag tgg Leu Glu Trp 150 ett gea gtt Leu Ala Val gee caa att Ala Gin Ile tac tac gaa Tyr Tyr Giu 200 ege ate gee Arg Ilie Ala 215 tte gge ace Phe Gly Thr 230 caa Gin ate Ile gta Val1 act Thr ggC Gly atc Ilie gga Gly 105 gta Vai gee Aia gee Ala tet Ser aag Lys 185 tee Ser eae His aaa Lys ctt Leu att Ile gat Asp gga Giy aaa Lys ggt Gly aa t Asn aae Asn gea Ala gea Ala gea Ala 170 geg Ala ggC Giy ete Leu gee Ala gaa Giu aca Thr aaa Lys ga t Asp gee Ala 75 ggt Gly tte Phe gee Ala gta Val1 atg Met 155 ege Arg att Ile tge Cys ac Thr eaa Gin 235 ate Ile aac Asn gaa Giu tee Ser 60 ate Ile tge Cys tgg Trp, gaa Glu ett Leu 140 tac Tyr gee Ala gaa Giu aac Asn tae Tyr 220 aag Lys eaa Gin get Ala gga.

Gly 45 aae Asn aae Asn aae Asn ggt Gly aaa Lys 125 gg t G ly cea Pro age Ser aae Asn eea Pro 205 cgt Arg aac Asn geg Ala gaa Glu 30 ege Arg gea Ala aet Thr ggt Gly aat Asn 110 e aa Gin ggt Giy gaa Giu gee Ala gac Asp 190 gee Ala gge Gly gaa Glu gg t Gly gee Ala aat Asn ga t Asp att Ilie 80 ac Thr t te Phe ete Leu atg Met gtt Val 160 eaa Gin eac His gga Gly eta Leu eea Pro 240 gat Asp tat Tyr gte Val tgg Trp tac Tyr gga Gly eee Pro gae Asp ggt Gly 145 gge Gly ate Ile tgg Trp ete Leu gaa Giu 225 etc Leu gte Val1 eac His gt t Val tgg Trp tgc Cys cc t Pro gee Ala gca Ala 130 gg t Gly gea Ala gge Gly cac His gge Gly 210 ate Ile ggt Gly tee Ser ege Arg etc Leu get Ala gtg Val1 Gly aeg Thr 115 etc Leu gee Ala get Ala att Ile gaa Giu 195 gee Ala gac Asp ccc Pro PCTLBOO/00923 ace gaa 163 Thr Glu tgg ggt 211 Trp Gly ate gaa 259 Ile Giu gac ttg 307 Asp Leu ate tgt 355 Ile Cys tee atg 403 Ser Met 100 tee att 451 Ser Ile ggc ate 499 Gly Ile ege ace 547 Arg Thr get gtt 595 Ala Val 165 caa tee 643 Gin Ser 180 gge aae 691 Gly Asn gee ega 739 Ala Arg gaa ege 787 Giu Arg tac ege 835 Tyr Arg 245 aag ccc gac cag ege tte gee gtg gaa tee tac ttg gac tac eaa gca WO 01/00843 WO 0100843PCT/IBOO/00923 Lys Pro Asp Gin Arg Phe Ala Val Giu Ser Tyr Leu Asp Tyr Gin Ala 250 255 260 gac aag cta gta cag cgt ttc gac gcc ggc tcc tac gtc ttg ctc acc 931 Asp Lys Leu Val Gin Arg Phe Asp Ala Gly Ser Tyr Val Leu Leu Thr 265 270 275 gac gcc ctc aac cgc cac gac att ggt cgc gac cgc gga ggc ctc aac 979 Asp Ala Leu Asn Arg His Asp Ilie Gly Arg Asp Arg Gly Gly Leu Asn 280 285 290 aag gca ctc gaa tcc atc aaa gtt cca gtc ctt gtc gca ggc gta gat 1027 Lys Ala Leu Giu Ser Ile Lys Val Pro Val Leu Val Ala Gly Val Asp 295 300 305 acc gat att ttg tac ccc tac cac cag caa gaa cac ctc tcc aga aac 1075 Thr Asp Ile Leu Tyr Pro Tyr His Gin Gin Giu His Leu Ser Arg Asn 310 315 320 325 ctg gga aat cta ctg gca atg gca aaa atc gta tcc cct gtc ggc cac 1123 Leu Gly Asn Leu Leu Ala Met Ala Lys Ile Val Ser Pro Val Gly His 330 335 340 gat gct ttc ctc acc gaa agc cgc caa atg gat cgc atc gtg agg aac 1171 Asp Ala Phe Leu Thr Giu Ser Arg Gin Met Asp Arg Ile Val Arg Asn 345 350 355 ttc ttc agc ctc atc tcc cca gac gaa gac aac cct tcg 1210 Phe Phe Ser Leu Ile Ser Pro Asp Glu Asp Asn Pro Ser 360 365 370 <210> 180 <211> 370 <212> PRT <213> Corynebacterium giutamicum <400> 180 Met Pro Thr Leu Ala Pro Ser Gly Gin Leu Giu Ile Gin Ala Ile Gly 1 5 10 Asp Val Ser Thr Glu Ala Gly Ala Ile Ile Thr Asn Ala Giu Ile Ala 25 Tyr His Arg Trp Gly Glu Tyr Arg Val Asp Lys Giu Gly Arg Ser Asn 40 Val Vai.Leu Ile Giu His Ala Leu Thr Gly Asp Ser Asn Ala Ala Asp 55 Trp Trp Ala Asp Leu Leu Gly Pro Giy Lys Ala Ile Asn Thr Asp Ile 70 75 Tyr Cys Val Ilie Cys Thr Asn Val Ile Gly Giy Cys Asn Gly Ser Thr 90 WO 01/00843 Giy Pro Gly Ser Met His Pro Asp C 100 1 Pro Ala Thr Ser Ile Arg Asp Gin 'v 115 120 Asp Ala Leu Gly Ile Thr Thr Val P~ 130 135 Giy Gly Ala Arg Thr Leu Glu Trp, P 145 150 Giy Ala Ala Ala Val Leu Ala Val S 165.

Ile Gly Ile Gin Ser Ala Gin Ile L 180 3 Trp, His Giu Gly Asn Tyr Tyr Giu E 195 200 Leu Giy Ala Ala Arg Arg Ile Ala F- 210 215 Giu Ilie Asp Giu Arg Phe Giy Thr I 225 230 Leu Giy Pro Tyr Arg Lys Pro Asp C 245 Leu Asp Tyr Gin Ala Asp Lys Leu 260 Tyr Val Leu Leu Thr Asp Ala Leu P~ 275 280 Arg Giy Gly Leu Asn Lys Ala Leu C 290 295 Val Ala Gly Val Asp Thr Asp Ile I 305 310 His Leu Ser Arg Asn Leu Gly Asn 1 325 Ser Pro Vai Gly His Asp Ala Phe L 340 Arg Ilie Vai Arg Asn Phe Phe Ser I 355 360 Pro Ser 370 <210> 181 <211> 771 <212> DNA <213> Corynebacterium giutamicum <22 0> PCTJI.BOO/00923 Phe Ala Val1 Met 155 Arg Ile Cys Thr Gin 235 Phe Arg His Ile Pro 315 Ala Giu S er TrP Giu Leu 140 Tyr Ala Giu Asn Tyr 220 Lys Ala Phe

ASP

Lys 300 Tyr Met Ser Pro Gly Lys 125 Gly Pro Ser Asn Pro 205 Arg Asn Vai Asp Ile 285 Val1 His Ala Arg Asp 365 WO 01/00843 WO 0100843PCTA~BOO/00923 <221> CDS <222> (101)..(748) <223> RXC01207 <400> 181 cttcatgatc tcaccggcag agcgcgtttt gttacagcgc gtaaactgtg actttgaaaa atttttgaac aatccgtaca ccaacttcag gagaaaaaca gtg agc aga atc tat Val Ser Arg Ile Tyr gac tgt gcc gac Asp Cys Ala Asp gac tcc cgt gca Asp Ser Arg Ala ggc cta aag gcg Gly Leu Lys Ala gct gtc Ala Val gat gca gtc ASP Ala Val tat gga ctc Tyr Gly Leu aaa gcc ggt cag ctc gtt gtc ctt ccc acg Lys Ala Gly Gin Leu Val Val Leu Pro Thr 30 ggc tgc gac gct ttc aac aac gag gca gta Gly Cys Asp Aia Phe Asn Asn Giu Ala Val gat acc cU: Asp Thr Leu gcc aac ctt Ala Asn Leu ctg gcc Leu Aia acc aaa cac cgt Thr Lys His Arg ccc gat atg ccc Pro Asp Met Pro cca gtg ctc gtc Pro Val Leu Val ggC Gly agc tgg gac acc Ser Trp Asp Thr att Ile caa gga ctt gtg Gin Gly Leu Val tcc tat tct gcg Ser Tyr Ser Ala gca aaa gcg ctt Ala Lys Aia Leu gtg Val1 gag gcg ttc tgg Glu Ala Phe Trp ggt gga ctg tc Gly Gly Leu Ser atc atc Ile Ile 100 gtt ccg cag Val Pro Gln acc gta atg Thr Val Met 120 gca Ala 105 cca agc ctt ccg Pro Ser Leu Pro aac ctt ggc gat Asn Leu Gly Asp acc cgt ggc Thr Arg Giy 115 gaa ttg ctg Glu Leu Leu ctg cgc atg cca Leu Arg Met Pro cac cca gtt gcc His Pro Val Ala att Ile 130 cgc caa Arg Gin 135 acc gga cca atg Thr Gly Pro Met gct Ala 140 gtc tcc tcc gcc Val Ser Ser Ala atc tcc gga cat Ile Ser Gly His cct cca acc acc Pro Pro Thr Thr ctg gag gct cgt Leu Glu Ala Arg cag Gln 160 cag ctc aac caa Gin Leu Asn Gin gtc gct gtc tac Val Ala Val Tyr ctc Leu 170 gat ggt ggc gaa Asp Gly Gly Glu gcg ctg gcc acc cct tca Ala Leu Ala Thr Pro Ser 180 acc atc gtg Thr Ile Val gat

ASP

185 att tca ggc ccc Ile Ser Gly Pro cca aag att ttg Pro Lys Ile Leu cgt gag ggt Arg Giu Gly 195 gcc atc agc gca gaa cgc gtt ggc gaa gta ctt gga gtg tcg gca gaa Ala le Ser Ala Giu Arg Val Gly Glu Val Leu Gly Val Ser Ala Giu WO 01/00843 WO 0100843PCT/IBOO/00923 200 205 210 agc ctg cgc taaatgggag tcggtttcgc ggg 771 Ser Leu Arg 215 <210> 182 <211> 216 <212> PRT <213> Corynebacterium giutarnicum <400> 182 Val Ser Arg Ile Tyr Asp Cys Ala Asp Gin Asp Ser Arg Ala Ala Gly 1 5 10 Leu Lys Ala Ala Val Asp Ala Val Lys Ala Gly Gin Leu Val Val Leu 25 Pro Thr Asp Thr Leu Tyr Gly Leu Gly Cys Asp Ala Phe Asn Asn Giu 40 Ala Val Ala Asn Leu Leu Ala Thr Lys His Arg Gly Pro Asp Met Pro 55 Val Pro Val Leu Val Gly Ser Trp Asp Thr Ilie Gin Gly Leu Val His 70 75 Ser Tyr Ser Ala Gin Ala Lys Ala Leu Val Giu Ala Phe Trp Pro Gly 90 Gly Leu Ser Ile Ile Val Pro Gin Ala Pro Ser Leu Pro Trp Asn Leu 100 105 110 Gly Asp Thr Arg Gly Thr Val Met Leu Arg Met Pro Leu His Pro Val 115 120 125 Ala Ilie Glu Leu Leu Arg Gin Thr Gly Pro Met Ala Val Ser Ser Ala 130 135 140 Asn Ile Ser Gly His Thr Pro Pro Thr Thr Val Leu Giu Ala Arg Gin 145 150 .155 160 Gin Leu Asn Gin Asn Val Ala Val Tyr Leu Asp Gly Gly Giu Cys Ala 165 170 175 Leu Ala Thr Pro Ser Thr Ilie Val Asp Ile Ser Gly Pro Ala Pro Lys 180 185 190 Ile Leu Arg Giu Gly Ala Ile Ser Ala Giu Arg Val Gly Glu Val Leu 195 200 205 Gly Val Ser Ala Giu Ser Leu Arg 210 215 <210> 183 <211> 1419 <212> DNA <213> Corynebacteriun giutainicum WO 01/00843 <220> <221> CDS <222> (101)..(1396) <223> RXC00152 <400> 183 gtcattgata tccaaggcac gaccgcgatt gtatggaaag PCTIBOO/00923 aagcctaaat ttttaacaat caaatagtac tggccattcc caactaaaac tggagtaacg atg aca gga cta atc Met Thr Gly Leu Ile gtt Val1 att Ile cgc Arg gta Val1 cag Gin gtg Val1 aac Asn 105 gtt Val atc Ile Giy tcc Ser cgc Arg 185 ttc Phe ccc Pro ac c Thr cgc Arg gc t Aia acc Thr tac Tyr gtc Val1 aac Asn ctg Leu atc Ile 170 gcc Ala c tg Leu cag Gin gtt Val gca Ala gtt Val1 ttc Phe atc Ilie ggt Gly cgc Arg cgc Arg 155 cag Gin acc Thr gtc Val gcc Ala 30 ggc Gly gac Asp caa Gin aac Asn gtg Val 110 acc Thr cgt Arg cgt Arg atg Met acc Thr 190 1 gtg Val att Ile ctg Leu gag Giu ctg Leu gag Giu att Ile gaa Giu ctc Leu 145 cta Leu cag Gin gg t Gly gtg Vai gaa Giu ctg Leu cgc Arg acc Thr cgc Arg tc t Ser *acc Thr 130 gat Asp aag Lys atg Met cag Gin atc Ile cgc Arg gtt Vai gtg Val1 gtg Val1 gc c Ala gta Val1 115 ctc Leu.

gca Ala gca Ala aag Lys cgc Arg 195 gac atc aaa act gcc gaa ggt gaa aag caa gcc aag atc ctc caa gct WO 01/00843 Asp Ile LVo 2C gag ggt ga Glu Gly Gi 215 gcg atg at Ala Met Il 230 gcg cag gq Ala Gin GI tct gcc aa Ser Ala Ly ctt cct ae Leu Pro L 2E agc cag tt 1027 Ser Gin P1~ 295 aag gat gc 1075 Lys Asp A] 310 gaa acc cc 1123 Giu Thr Ax acc gaa tc 1171 Thr Giu SE gtg gcc az 1219 Val Ala AE 3( aag acc gc 1267 Lys Thr A] 375 aac gga ac 1315 Asn Gly T1 390 aac ttc gc 1363 Ala cac His cgc Arg gcc Ala 250 acc Thr gca Ala gat Asp ggt Gly atc Ile 330 cct Pro cc a Pro cgc Arg aca Thr gaa Glu gca Ala gcc Ala 235 cga Arg cca Pro gag Glu tc t Ser gtC Val1 315 gca Ala gaa Giu gtc Val gtt Val caa Gin 395 ttc Gly tcc Ser 220 gaa.

Giu gca Ala gag Giu G ly ctg Leu 300 ttc Phe aac Asn atc Ile gat Asp gaa Giu 380 cc t Pro cc t Leu gaa Giu tac Tyr gca Ala c tc Leu 275 gtc Val1 ttc Phe gtg Val1 tgg Trp gca Ala 355 ctt Leu gct Ala cct Pro gaa PCTIBOOIOO923 Gin Ala cgc caa 787 Arg Gin ctc cag 835 Leu Gin 245 atc aag 883 Ile Lys 260 gaa aag 931 Giu Lys atc cca 979 Ile Pro ggc gca Gly Ala gat gaa Asp Glu 325 ttc tcC Phe Ser 340 aac gcc Asn Ala tcc aag Ser Lys ttg caa Leu Gin acc gca Thr Ala 405 gat tac Asn Phe Ala Gin Giu Phe Pro Ala Pro Gin Ala Asn Pro Glu Asp Tyr WO 01/00843 WO 0100843PCTIBOOIOO923 410 415 420 tcc gac caa cac cga gag aat cct tac gga aac taatcaggca taagaaaagg 1416 Ser Asp Gin H-is Arg Glu Asn Pro Tyr Gly Asn 425 430 Cgg 1419 <210> 184 <211> 432 <212> PRT <213> Corynebacterium giutamicun <400> 184 Met Thr Gly Leu Ile Leu Ala Ile Val Phe Leu Val Phe Val Ala Val 1 5 10 Val Val Ile Lys Ser Ile Ala Leu Ile Pro Gin Gly Giu Ala Ala Val 25 Ile Giu Arg Leu Gly Ser Tyr Thr Arg Thr Val Ser Gly Gly Leu Thr 40 Leu Leu Val Pro Phe Val Asp Arg Val Arg Ala Arg Ile Asp Thr Arg 55 Giu Arg Val Val Ser Phe Pro Pro Gin Ala Val Ile Thr Gin Asp Asn 70 75 Leu Thr Val Ala Ile Asp Ile Val Val Thr Phe Gin Ile Asn Giu Pro 90 Giu Arg Ala Ile Tyr Gly Val Asp Asn Tyr Ile Val Gly Val Giu Gin 100 105 110 Ile Ser Val Ala Thr Leu Arg Asp Val Val Gly Gly Met Thr Leu Glu 115 120 125 Giu Thr Leu Thr Ser Arg Asp Val Ile Asn Arg Arg Leu Arg Gly Glu 130 135 140 Leu Asp Ala Ala Thr Thr Lys Trp Gly Leu Arg Ile Ser Arg Val Giu 145 150 155 160 Leu Lys Ala Ile Asp Pro Pro Pro Ser Ile Gin Gin Ser Met Giu Lys 165 170 175 Gin Met Lys Ala Asp Arg Giu Lys Arg Ala Thr Ile Leu Thr Ala Giu 180 185 190 Gly Gin Arg Giu Ala Asp Ile Lys Thr Ala Giu Gly Giu Lys Gin Ala 195 200 205 Lys Ile Leu Gin Ala Giu Gly Glu Lys His Ala Ser Ile Leu Asn Ala 210 215 220 Glu Ala Giu Arg Gin Ala Met Ile Leu Arg Ala Giu Gly Giu Arg Ala 225 230 235 240 WO 01/00843 WO 0100843PCT/EBOO/00923 Ala Arg Tyr Leu Gin Ala Gin Gly Giu Ala Arg Ala Ile Gin Lys Val 245 250 255 Asn Ala Ala Ile Lys Ser Ala Lys Leu Thr Pro Glu Val Leu Ala Tyr 260 265 270 Gin Tyr Leu Giu Lys Leu Pro Lys Ile Ala Glu Gly Asn Ala Ser Lys 275 280 285 Met Trp Val Ile Pro Ser Gin Phe Ser Asp Ser Leu Giu Gly Phe Ala 290 295 300 Lys Gin Phe Gly Ala Lys Asp Ala Giu Gly Val Phe Arg Tyr Giu Pro 305 310 315 320 Asn Thr Val Asp Giu Glu Thr Arg Asp Ile Ala Asn Ala Asp Asn Val 325 330 335 Glu Asp Trp Phe Ser Thr Giu Ser Asp Pro Glu Ilie Ala Ala Ala Val 340 345 350 Ala Ala Ala Asn Ala Val Ala Asn Lys Pro Val Asp Pro Glu Pro Gly 355 360 365 Glu Ilie Leu Ser Lys Lys Thr Ala Arg Arg Val Glu Pro Giu Ala Val 370 375 380 Leu Glu Ala Leu Gin Asn Gly Thr Thr Thr Gin Pro Giu Val GlU Ala 385 390 395 400 Ala Pro Pro Thr Ala Asn Phe Ala Gin Glu Phe Pro Ala Pro Gin Ala 405 410 415 Asn Pro Glu Asp Tyr Ser Asp Gin His Arg Giu Asn Pro Tyr Gly Asn 420 425 430 <210> 185 <211> 1170 <212> DNA <213> Corynebacterium glutarnicum <220> <221> CDS <222> (101)..(1147) <223> RXA00115 <400> 185 tggattctcg agtctgtaca cccttgatca aagcccgagt gttccgtaga ttaactttgt cgtatattgt gacctacacc ccatactgtt aggagttttc atg ctc gac aat agt 115 Met Leu Asp Asn Ser 1 ttt tac acc gca gag gtt cag ggc cca tac gaa acc gct tcc att ggc 163 Phe Tyr Thr Ala Giu Val Gin Gly Pro Tyr Glu Thr Ala Ser Ile Gly 15 WO 01/00843 WO 0100843PCT/iBOO/00923 cgg ctc gaa ctc gaa gaa ggg ggt gtg att gag gat tgc Arg Leu Giu Leu Giu Glu Gly Gly Val Ile Giu Asp Cys 30 tac Tyr att Ile atc Ile atc Ile ga t Asp gat Asp gag Giu gag Giu 150 gcg Ala ctt Leu tcc Ser tgg Trp cg t Arg 230 gc t Ala tgg Trp gat Asp atc Ile atc Ile 105 gcc Ala gcc Ala gtt Val1 gcg Ala acc Thr 185 gaa Giu atg Met gga Giy gga Gly tac Tyr cat His gg t Giy tcg Ser cag Gin gtc Val1 cgc Arg aag Lys 170 gtt Val1 gag Glu gga Gly ctt Leu acg Thr tcc Ser gcg Ala 75 aat Asn atg Met gac Asp gt t Val1 t tc Phe 155 aac Asn gag Giu gta Val1 ttt Phe gaa Giu 235 aac Asn acc Thr gat Asp t tg Leu aag Lys ctc Leu 125 gg t Giy gac Asp cc t Pro gat Asp ga t Asp 205 aca Thr aag Lys gag Glu cat His cca Pro tcg Ser ttc Phe 110 t tg Leu tcg Ser caa Gin cat His c ca Pro 190 gga Gly gag Giu gag Giu gac Asp cag Gin tca Ser gtc Val 95 ccg Pro cgc Arg atg Met gtt Val gat Asp 175 ggg Gly ctt Leu t tc Phe tca Ser aat Asn 255 aag Lys acc Thr aag Lys 80 tcc Ser aa t Asn caa Gin ggt Giy cat His 160 t tc Phe t tc Phe cgc Arg tgg Trp gtg Val 240 acc Thr tcc Ser tgg Trp tat Tyr cct Pro gt t Vali gag Giu gcg Ala 145 cga Arg atc Ile aat Asn cgt Arg aag Lys 225 ctc Leu ttg Leu aac Asn ttc Phe ttc Phe gcc Ala cgc Arg ttt Phe 130 cag Gin gca Ala ttc Phe ggc Gly caa Gin 210 cag Gin gcg Ala ctc Leu tgg ttg Trp Leu gcc atc Ala Ile cag cag Gin Gin atc atc Ile Ile aac acg Asn Thr 100 att ggt Ile Gly 115 ggt att Gly Ile caa acc Gin Thr gct ccg Ala Pro acc cag Thr Gin 180 ggc gaa Gly Giu 195 tcg cat Ser His gag gca Giu Ala gac ttc Asp Phe aac aac Asn Asn 260 gc t Ala ctc Leu tac Tyr tcc Ser gc t Aila gat Asp acc Thr tat TPyr atc Ile 165 act Tfir tac Tyr ctt Leu tgg Trp c tg Leu 245 gc t Ala gat ccg ctg ttc atg tcc atg gat cct ASP Pro Leu Phe Met Ser Met Asp Pro 250 WO 01/00843 tgg aag tgg Trp Lys Trp gca gcg gct Ala Ala Ala 280 agc gag gac 1027 Ser GiU Asp 295 ctc atc cca 1075 Leu Ile Pro 310 ctt ggg ctt 1123 Leu Gly Leu aat ctg aaa 1170 Asn Leu Lys PCTI.BOO/00923 tct cgc cac acc ggc ggc gac ttg 931 Ser Arg His Thr Gly Gly Asp Leu 270 275 gct aag acc ttc gtt atg ccc atc 979 Ala Lys Thr Phe Val Met Pro Ile 290 cgt gac tgt gcc gca gaa caa gca Arg Asp Cys Ala Ala Giu Gin Ala 305 gtg atc gaa gac atc gcc ggt cac Val Ile Giu Asp Ile Ala Gly His 320 325 aat tac atc cca cag atc gac aaa Asn Tyr Ile Pro Gin Ile Asp Lys 335 340 taaacactga tgtcaaagag cct <210> 186 <2i1> 349 <212> PRT <213> Corynebacteriun glutamicum <400> 186 Met Leu Asp Asn Ser Phe Tyr Thr Ala 1 5 Thr Ala Ser Ilie Gly Arg Leu Giu Leu Asp Cys Trp Leu Ala Tyr Ala Thr Aia Ser Asn Ala Ilie Leu Ile Pro Thr Trp 55 Trp Phe Gin Gin Tyr Ilie Giy Thr Asp 70 Tyr Phe Ile Ile Ser Ile Asn Gin Ile Pro Ala Asn Thr Ala Asp Asp Ser Ile 100 105 Val Arg Ile Giy Asp Asp Val Vai Ala 115 120 Giu Phe Giy Ile Thr Giu Leu Phe Ala 130 135 Giu Giu Gly Tyr His Gly Ser Gin Val Val1 Giu Thr Ser Ala Asn Met Asp Val1 Gin Gly Leu Giy Leu Gly S er Arg Gly 140 WO 01/00843 WO 0100843PCTIiBOO/00923 Ala Gin Gin Thr Tyr Giu Trp Ile Val Arg Phe Pro Asp Gin Vai His 145 150 155 160 Arg Ala Ala Pro Ile Ala Gly Thr Ala Lys Asn Thr Pro His Asp Phe 165 170 175 Ile Phe Thr Gin Thr Leu Asn Giu Thr Val Giu Ala Asp Pro Gly Phe 180 185 190 Asn Gly Giy Giu Tyr Ser Ser His Glu Giu Val Ala Asp Gly Leu Arg 195 200 205 Arg Gin Ser His Leu Trp Aia Ala Met Gly Phe Ser Thr Giu Phe Trp 210 215 220 Lys Gin Giu Ala Trp Arg Arg Leu Gly Leu Giu Ser Lys Giu Ser Vai 225 230 235 240 Leu Ala Asp Phe Leu Asp Pro Leu Phe Met Ser Met Asp Pro Asn Thr 245 250 255 Leu Leu Asn Asn Ala Trp Lys Trp Gin His Gly Asp Val Ser Arg His 260 265 270 Thr Gly Giy Asp Leu Ala Ala Ala Leu Gly Arg Val Lys Ala Lys Thr 275 280 285 Phe Val Met Pro Ile Ser Giu Asp Met Phe Phe Pro Val Arg Asp Cys 290 295 300 Ala Ala Giu Gin Ala Leu Ile Pro Gly Ser Giu Leu Arg Val Ile Glu 305 310 315 320 Asp Ilie Ala Gly His Leu Gly Leu Phe Asn Val Ser Giu Asn Tyr Ile 325 330 335 Pro Gin Ilie Asp Lys Asn Leu Lys Glu Leu Phe Giu Ser 340 345 <210> 187 <211> 1254 <212> DNA <213> Corynebacterium glutamicun <22 0> <221> CDS <222> (1231) <223> RXN00403 <400> 187 *tttttcagac tcgtgagaat gcaaactaga ctagacagag ctgtccatat acactggacg aagttttagt cttgtccacc cagaacaggc ggttattttc atg ccc acc ctc gcg 115 Met Pro Thr Leu Ala 1 cct tca ggt caa ctt gaa atc caa gcg atc ggt gat gtc tcc acc gaa 163 Pro Ser Gly Gin Leu Glu Ile Gin Ala Ile Gly Asp Val Ser Thr Giu 15 WO 01/00843 WO 0100843PCT/1BO0100923 gcc Ala gaa Giu cac His ctc Leu acc Thr cat His cgt Arg acc Thr cta Leu 150 ctt Leu gcc Ala tac Tyr cgc Arg ttc Phe 230 aag Lys gga gca Gly Ala tac cgc Tyr Arg gcc ctc Ala Leu ggt ccc Gly Pro aac gtc Asn Val cca gat Pro Asp gat cag Asp Gin 120 acg gtc Thr -Val 135 gag tgg Giu Trp gca gtt Ala Val caa att Gin Ile tac gaa Tyr Glu 200 atc gcc Ile Ala 215 ggc acc Giy Thr ccc gac Pro Asp atc Ile gta Val1 act Thr ggc Gly a tc Ile gga Gly 105 gta Val gcc Ala gcc Ala tc t Ser aag Lys 185 tcc Ser cac His aaa Lys cag Gin att Ile gat Asp gga Giy aaa Lys ggt Giy aat Asn aac Asn gca Ala gca Ala gca Ala 170 gcg Ala ggc Gly ctc Leu gcc Ala cgc Arg 250 aca Thr aaa Lys gat Asp gcc Ala 75 ggt Gly ttc Phe gcc Ala gta Val atg Met 155 cgc Arg att Ile tgc Cys acc Thr caa.

Gin 235 ttc Phe aac Asn gaa Glu tcc Ser 60 atc Ile tgc Cys tgg Trp gaa Giu ct t Leu 140 tac Tyr gcc Al a gaa Giu aac Asn tac Tyr 220 aag Lys gcc Ala atc Ile agc Ser gcc Ala gat Asp tcc S er 95 cgc Arg ttc Phe tcc Ser act Thr tgg Trp 175 cac His acc Thr gaa Giu aac Asn gcc Al a aat Asn gat Asp att Ile 80 acc Thr t tc Phe ctc Leu atg Met gt t Val1 160 caa Gin cac His gga Gly cta Leu cca Pro 240 tat Tyr gtc Val tgg Trp t ac Tyr gga Gly ccc Pro gac Asp gg t Gly 145 ggc Giy atc Ile tgg Trp ctc Leu gaa G iu 225 c tc Leu cac His gtt Val1 tgg Trp tgc Cys cc t Pro gcc Ala gca Ala 130 ggt Gly gca Ala ggc Gly cac His ggc Gly 210 aic Ile ggt Gly cgc Arg ctc Leu gc t Ala gtg Val1 ggc Gly acg Thr 115 ctc Leu gcc Ala gct Ala att Ile gaa Giu 195 gcc Ala gac Asp ccc Pro tgg Trp atc Ile gac Asp atc Ile t cc Ser 100 tcc Ser ggc Gly cgc Arg gc t Ala caa Gin 180 ggc Gly gcc Ala gaa Glu tac Tyr ggt Gly gaa Glu ttg Leu tgt Cys atg Met att Ile atc Ile acc Thr gt t Val1 165 tcc Ser aac Asn cga Arg cgc Arg cgc Arg 245 tcc tac ttg gac tac caa gca Ser Tyr Leu Asp Tyr Gin Ala 255 260 WO 01/00843 gac aag ct Asp Lys L( gac gcc ct Asp Ala L( aag gca ct 1027 Lys Ala L( 295 acc gat at 1075 Thr Asp I] 310 ctg gga aE 1123 Leu Gly Ac gat gct tt 1171 Asp Ala P1 ttc ttc ac~ 1219 Phe Phe S( gta Val1 265 aac Asn gaa Glu ttg Leu cta Leu ctc Leu 345 ctc Leu cag cgt Gin Arg cgc cac Arg His tcc atc Ser Ile tac ccc Tyr Pro 315 ctg gca Leu Ala 330 acc gaa Thr Glu atc tcc ttc gac Phe Asp gac att Asp Ile 285 aaa gtt Lys Val 300 tac cac Tyr His atg gca Met Ala agc cgc Ser Arg cca gac gcc Ala 270 ggt Gly cca Pro cag Gin aaa Lys caa Gin 350 gaa tcc tac Ser Tyr gac cgc Asp Arg ctt gtc Leu Val 305 gaa cac Giu His 320 gta tcc Val Ser gat cgc Asp Arg aac cct Asn Pro gtc ttg Val Leu 275 gga ggc Gly Gly 290 gca ggc Ala Gly ctc tcc Leu Ser cct gtc Pro Val atc gtg Ile Val 355 tcg acc Ser Thr 370 PCT/1B00100923 ctc acc 931 Leu Thr ctc aac 979 Leu Asn gta gat Val Asp aga aac Arg Asn 325 ggc cac Gly His 340 agg aac Arg Asn tac atc Tyr Ile Ile Ser Pro Asp Giu Asp 365 gag ttc tac atc taataggtat ttacgacaa 1254 Glu Phe Tyr Ile 375 <210> 188 <211> 377 <212> PRT <213> Corynebacteriun glutaiicun <400> 188 Met Pro Thr Leu Ala Pro Ser Gly Gin 1 5 Asp Val Ser Thr Glu Ala Gly Ala Ile Tyr His Arg Trp Gly Glu Tyr Arg Val 40 Val Val Leu Ilie Giu His Ala Leu Thr Trp Trp Ala Asp Leu Leu Gly Pro Gly 70' Tyr Cys Val Ile Cys Thr Asn Val Ile 275 .a tag Leu G Ile T~ Asp L: Gly A~ Lys A Giy G iu hr s la ly WO 01/00843 Gly Pro Asp Gly 145 Gly Ile Trp Leu Glu 225 Leu Leu Tyr Arg Val 305 His Ser Arg Pro Ala Ala 130 Gly Ala Gly His Gly 210 Ile Gly Asp Val Giy 290 Ala Leu Pro Ile Gly Thr 115 Leu Ala Ala Ile Glu 195 Ala Asp Pro Tyr Leu 275 Gly Gly Ser Val Val 355 Ser 100 Ser Gly Arg Ala Gin 180 Gly Ala Giu Tyr Gin 260 Leu Leu Val1 Arg Gly 340 Arg Met Ilie Ile Thr Val1 165 Ser Asn Arg Arg Arg 245 Ala Thr Asn

ASP

Asn 325 His Asn Trp Glu Leu 140 Ala Giu Asn Tyr 220 Lys Ala Phe Asp Lys 300 Tyr Met Ser Pro Gly Lys 125 Gly Pro Ser Asn Pro 205 Arg Asn Val1 Asp Ile 285 Val His Ala Arg Asp 365 Asn 110 Gin Gly Giu Ala Asp 190 Ala Gly Giu Giu Ala 270 Gly Pro Gin Lys Gin 350 Giu PCTIiBOO/00923 Arg Phe Phe Leu Ser Met Thr Val 160 Trp Gin 175 His His Thr Gly Giu Leu Asn Pro 240 Ser Tyr 255 Gly Ser Arg Asp Val Leu Gin Giu 320 Ile Val 335 Met Asp Asp Asn Pro Ser Thr Tyr Ilie Glu Phe Tyr Ile 370 375 <210> 189 <211> 1210 <212> DNA <213> Corynebacterium giutanicun WO 01/00843 WO 0100843PCTIBOOIOO923 <220> <221> CDS <222> (101)..(1210) <223> FRXA00403 <400> 189 tttttcagac tcgtgagaat gcaaactaga ctagacagag ctgtccatat acactggacg aagttttagt cttgtccacc cagaacaggc ggttattttc atg ccc acc ctc Met Pro Thr Leu 1 cct tca ggt caa Pro Ser Gly Gin ctt Leu gaa atc caa gcg Glu Ile Gin Ala ggt gat gtc tcc Gly Asp Val Ser acc gaa Thr Glu gcc gga gca Ala Gly Ala gaa tac cgc Glu Tyr Arg att aca aac gct Ile Thr Asn Ala atc gcc tat cac Ile Ala Tyr His cgc tgg ggt Arg Trp Gly ctc atc gaa Leu Ile Glu gta gat aaa gaa Val Asp Lys Giu gga Gly 45 cgc agc aat gtc Arg Ser Asn Val gtt Val1 cac gcc His Ala ctc act gga gat Leu Thr Giy Asp aac gca gcc gat Asn Ala Ala Asp tgg gct gac ttg Trp Ala Asp Leu ggt ccc ggc aaa Gly Pro Gly Lys atc aac act gat Ile Asn Thr Asp tac tgc gtg atc Tyr Cys Val Ile acc aac gtc atc Thr Asn Val Ile ggt Gly ggt tgc aac ggt Gly Cys Asn Gly acc gga cct ggc Thr Gly Pro Gly tcc atg Ser Met 100 cat cca gat His Pro Asp cgt gat cag Arg Asp Gin 120 aat ttc tgg ggt Asn Phe Trp Giy cgc ttc ccc gcc Arg Phe Pro Ala acg tcc att Thr Ser Ilie 115 ctc ggc atc Leu Gly Ile gta aac gcc gaa Val Asn Ala Glu caa ttc ctc gac Gln Phe Leu Asp acc acg Thr Thr 135 gtc gcc gca gta Val Ala Ala Val ggt ggt tcc atg ggt ggt gcc cgc acc Gly Gly Ser Met Gly Gly Ala Arg Thr 145 gag tgg gcc gca Giu Trp Ala Ala atg Met 155 tac cca gaa act Tyr Pro Glu Thr ggc gca gct gct Gly Ala Ala Ala ctt gca gtt tct Leu Ala Val Ser gca Ala 170 cgc gcc agc gcc Arg Ala Ser Ala caa atc ggc att Gln Ilie Gly Ile caa tcc Gln Ser 180 gcc caa att Ala Gln Ile gcg att gaa aac Ala Ile Glu Asn cac cac tgg cac His His Trp His gaa ggc aac Glu Gly Asn 195 WO 01/00843 tac tac gaa Tyr Tyr Giu 200 cgc atc gcc Arg Ile Ala 215 ttc ggc acc Phe Gly Thr 230 aag ccc gac Lys Pro Asp gac aag cta Asp Lys Leu gac gcc ctc Asp Ala Leu 280 aag gca ctc 1027 Lys Ala Leu 295 acc gat att 1075 Thr Asp Ile 310 ctg gga aat 1123 Leu Gly Asn gat gct ttc 1171 Asp Ala Phe ttc ttc agc 1210 Phe Phe Ser 360 ggC Gly ctc Leu gcc Ala cgc Arg 250 cag Gin cgc Arg tcc Ser tac Tyr ctg Leu 330 acc Thr atc gcc Ala ggc Gly gaa Glu gaa Giu gcc Ala 270 ggt Gly cca Pro cag Gin aaa Lys caa Gin 350 gaa acc Thr gaa Glu aac Asn tcc Ser 255 ggc Gly cgc Arg gtc Val caa Gin atc Ile 335 atg Met gac gga Gly c ta Leu cca Pro 240 tac Tyr tcc Ser gac Asp ct t Leu gaa Giu 320 gta Val1 ga t Asp aac PCTJIBOO/00923 gcc.cga 739 Ala Arg gaa cgc 787 Giu Arg tac cgc 835 Tyr Arg 245 caa gca 883 Gin Ala 260 ctc acc 931 Leu Thr ctc aac 979 Leu Asn gta gat Val Asp aga aac Arg Asn .325 ggc cac Gly His 340 agg aac Arg Asn Leu Ile Ser Pro GiU Asp Asn Pro Ser 370 <210> 190 <211> 370 <212> PRT <213> Corynebacterium glutamicun <400> 190 Met Pro Thr Leu Ala Pro Ser Gly Gin Leu Giu Ile Gin Ala Ile Gly 1 5 10 Asp Val Ser Thr Glu Ala Gly Ala Ile Ile Thr Asn Ala Glu Ile Ala 25 278 WO 01/00843 WO 0100843PCTIIBOO/00923 Tyr Val Trp Tyr Gly Pro Asp Gly 145 Gly Ile Trp Leu Giu 225 Leu Leu Tyr Arg Val 305 His Ser His Val1 Trp Cys Pro Ala Ala 130 Gly Ala Gly His Gly 210 Ile Gly Asp Val1 Gly 290 Ala Leu Pro Tip Gly Glu Tyr Val Asp Lys Glu Giu Leu Cys Met Ile Ile Thr Val 165 Ser Asn Arg Arg Arg 245 Ala Thr Asn Asp Asn 325 His His Leu 70 Thr His Arg Thr Leu 150 Leu Ala Tyr Arg Phe 230 Lys Asp Asp Lys Thr 310 Leu Asp Gly Lys G ly Asn Asn Ala Ala Ala 170 Ala Gly Leu Ala Arg 250 Gin Arg Ser Tyr Leu 330 Thr Asp Ala 75 Gly Phe Ala Val Met 155 Arg Ile Cys Thr Gin 235 Phe Arg His Ile Pro 315 Ala Glu Ser Ile Cys Trp Giu Leu 140 Tyr Ala Giu Asn Tyr 220 Lys Ala Phe Asp Lys 300 Tyr Met Ser Gly Asn Asn Asn Gly Lys 125 Gly Pro Ser Asn Pro 205 Arg Asn Val1 Asp Ile 285 Val His Ala Arg Arg Ser Asn Asp Ile Thr Phe Leu Met Val1 160 Gin His Gly Leu Pro 240 Tyr Ser Asp Leu Giu 320 Val1 Asp WO 01/00843 PCTI1B00100923 Arg Ile Val. Arg Asfl Phe Phe Ser Leu Ile Ser Pro Asp Giu Asp Asn 355 360 365 Pro Ser 370 .<210> 191 <211> 687 <212> DNA <213> Corynebacterium giutanicun <220> <221> CDS <222> (101)..(664) <223> RXS03158 <400> 191 caaagctcac cgaaggcacc aacgccaagt tggttgttga caacaccttg gcatccccat acctgcagca gccactaaaa ctcggcgCac acgcaagtcc ttg cac tcc acc acc Leu His Ser Thr Thr aag Lys aac Asn -gga Gly aag Lys atc Ile cca Pro aag Lys gca Ala tcc Ser tcc Ser gaa Glu ttc Phe atg Met tcc Ser cca Pro atc Ile acc Thr tcc Ser 140 gac Asp gaa Glu gat

ASP

gat Asp cgc Arg ggc Gly tcc Ser t cc Ser 125 ctc Leu gt t Val c tg Leu tac Tyr c ac His gag Glu gaa Giu cgt Arg aaa Lys gag Giu ggc Gly ttc Phe c tg Leu tgc Cys gtc Val gtc Val1 ttc Phe ctg Leu cac His 1 ggc Gly atg Met ac c Thr gac Asp tcc Ser gca Ala gca Ala atc Ile cca Pro 145 ggt Gly ggc Gly ggc Gly gaa Giu ctc Leu cag Gin 100 gaa Giu gcc Ala atg Met 115 163 211 259 307 355 403 451 499 547 595 cac cag tca gct gcc ggc tct cag ctc gag gtt ccc cgc gac ctc gtg WO 01/00843 His Gin Ser 150 cgc atc tcc Arg Ilie Ser gag cag gcc Giu Gin Ala PCTAIBOO/00923 Ala gg t Gly 170 aa t Asn Gly Ser 155 att gaa Ile Giu aac ctt Asn Leu Gin Leu Giu Val Pro Arg Asp Leu Vai 160 165 gac att gaa gac ctg ctc gca gat gtc 643 Asp Ilie Giu Asp Leu Leu Ala Asp Val 175 180 tagaaactat ttggcggcaa gca 687 <210> i92 <2i1> 188 <2i2> PRT <213> Corynebacteriun giutamicun <400> 192 Leu His Ser Thr Thr Lys Tyr Ile Glu Giy Met Thr Asp Ser Aia Aia Ile Pro 145 Pro Leu Leu Gin Aia Asn Thr Ala Giy Cys 130 Aia Arg Leu Val Giy Arg Aia Vali Lys Gly 115 Leu Thr Asp Ala Giy G ly Giy Glu Leu Gin 100 Giu Ala Met Leu Asp 180 Thr Ilie Leu Lys Tyr Met Giu Giu Thr Val 165 Val1 Asn Giy Lys Ile 70 Pro Lys Ala Ser His 150 Arg Glu Gin Ile 40 Leu Giu Leu Phe Lys 120 Giy Ser Ser Ala His Asp Val Arg Asp 75 His Met Cys Giu Gly 155 Ile Asn Ser Giu Phe met Ser Pro Ile Thr Ser 140 Ser Giu Leu Asp Giu Asp Asp Arg Gly Ser Ser 125 Leu Gin Asp Val Leu Ala Arg Pro His Val1 110 Thr Leu Leu Ile Val1 Leu Tyr His Giu Glu Arg Lys Giu Giu Giu 175 Giy Phe Leu Cys Val Val1 Phe Leu His Vai 160 Asp <210> i93 <211> 6i7 <2i2> DNA <213> Corynebacteriun giutamicui <220> WO 01/00843 <221> CDS <222> <223> FRXA00254 PCTIIB0O/00923 <400> 193 cag cca c Gin Pro L 1 tac atc g Tyr Ile G gac cag g Asp Gin G ccg atc c Pro Ile P acc ctt g Thr Leu A gcg gaa t Aia Giu P ggt ctg a Giy Leu L cgc ttc g~ Arg Phe G i gct aag a Ala Lys L 130 Ctc ggt g~ Leu Gly G 145 cag tca g~ Gin Ser A atc tcc a Ile Ser I cag gcc c Gin Ala L, 1 <210> 194 <211> 198 <212> PRT C tC Leu 5 cac His gac Asp gt t Vali cgc Arg gac Asp c ac His atg Met tgt Cys gaa Giu ggC G ly 165 att Ile ggc Giy tcc Ser gaa Giu ttc Phe atg Met 70 tcc Ser cca Pro atc Ile acc Thr tcc Ser 150 tc t Ser gaa Glu gca Ala gac Asp gaa Giu gat Asp gat Asp cgc Arg ggc Gly tcC Ser tcc Ser 135 ctc Leu cag Gin gac Asp cac gca His Aia gtt gtt Vai Val ctg ctg Leu Leu gca tac Ala Tyr cgc cac Arg His cca gag Pro Giu cac gaa His Giu 105 gtc cgt Val Arg 120 acc aaa Thr Lys ctg gag Leu Glu ctc gag Leu Giu att gaa Ile Glu ttg Leu ggC Gly atg Met ac c Thr gac Asp tcc Ser gca Ala gca Ala a tc Ile C Ca Pro 155

CCC

Pro c tg tCC Ser gtg Val1 ggc Gly Cgt Arg gca Ala gtg Val aag Lys ggc Giy 125 Ctg Leu aCC Thr gac Asp gca aCC Thr gtt Val 9gC Gly ggc Gly gaa Giu ctc Leu Cag Gin 110 gaa Glu gCC Ala atg Met CtC Leu gat Asp 190 8CC Thr aCC Thr atc Ile ctc Leu aag Lys taC Tyr atg Met gaa Giu gag Giu aCC Thr gtg Vai 175 gtC Val1 48 96 144 192 240 288 336 384 432 480 528 576 Asp Leu Leu Ala aat aaC Ctt Asn Asn Leu tagaaactat ttggcggcaa gca WO 01/00843 <213> Corynebacterium giutamicum <400> 194 Gin Pro Leu Lys Leu Gly Ala His Ala PCTIBOO/00923 1 Tyr Asp Pro Thr Ala Gly Arg Ala Leu 145 Gin Ilie Gin Ile Gin Ilie Leu Giu Leu Phe Lys 130 Gly Ser Ser Ala Gly Giu Pro Ala Phe Lys Giy 115 Lys Gly Ala Ile Leu 195 5 His Asp Val1 Arg Asp His Met Cys Giu Gly 165 Ile Asn Ser Giu Phe Met 70 Ser Pro Ile Thr Ser 150 Ser G iu Leu Val Leu Tyr His Giu G iu 105 Arg Lys Giu Giu Giu 185 Val1 10 Gly Phe Leu Cys Val 90 Val Phe Leu His Val1 170 Asp Leu Gly Met Thr Asp 75 Ser Ala Ala Ile Pro 155 Pro Leu His Leu Gin Ala Asn Thr Ala Gly Cys 140 Ala Arg Leu Thr Val1 Gly Gly Giu Leu Gin 110 Giu Ala Met Leu Asp 190 Thr Thr Ile Leu Lys Tyr Met Giu Giu Thr Val1 175 Val Lys Asn Gly Lys Ile Pro Lys Ala Ser His 160 Arg Glu <210> 195 <21i> 1170 <212> DNA <213> Corynebacteriun giutanicuin <2 <221> CDS <222> (101)..(1147) <223> RXA02532 <400> 195 gatgaatttt tacccaccat ctgtacctat taaccctgcg tggcgtccac ccacagtaac tgtgcaagcg ggacggccag ccagaactcc tggtgcgccg atg aac cca cct atc 115 Met Asn Pro Pro Ile 1 acg ttg tcc agc act tat gtt cat gat tca gaa aaa gct tat ggg cgc 163 283 WO 01/00843 Thr Leu Ser Ser Thr Tyr Val His Asp Ser Glu Lys Ala Tyr 15 ga t Asp ga t Asp t cg Ser gct Ala gga Gly att Ilie aat Asn cgt Arg ctt Leu 150 gca Ala gtg Val gat Asp gga Gly gca Ala 230 ggc Gly gg t Gly att Ile gcc Ala agg Arg gct Ala ccg Pro ggg Gly 135 cg t Arg acc Thr tgc Cys cat His ttg Leu 215 gaa Glu aat Asn ggg Gly gc t Ala tat Tyr ctg Leu gc t Ala acg Thr 120 ctt Leu caa Gin aaa Lys aag

LYS

ggt Gly 200 tat Tyr ctt Leu gat

ASP

t tc Phe ga t Asp tat Tyr aag Lys gct Ala 105 atg Met gga Giy cgt Arg ctt Leu tct Ser 185 tca Ser tcC Ser tcg Ser gga Gly gcg Ala ttg Leu ggc Gly gt t Val caa Gin gtg Val gt t Val cca Pro atc Ile 170 gag Giu gtg Val t tg Leu cgg Arg tgg Trp, gta Val gtt Val gtg Val 75 cga Arg gg t G ly gta Val t tg Leu t tg Leu 155 ggt Gly c ac His ccg Pro gcg Ala cga Arg 235 ggt Gly tct Ser cct Pro 60 acc Thr act Thr gca Ala gc t Ala act Thr 140 gaa Glu gga Gly cat His gga Gly gtg Val 220 ctt Leu gca Ala tat Tyr 45 act Thr aat Asn gtt Val1 ga t Asp gat Asp 125 gtc Val1 ctt Leu cac His gcg Ala gg t Gly 205 cgt Arg aac Asn ttt Phe 30 tct Ser ggt Gly att Ile gat Asp gtg Val1 110 atc Ile gtt Val ggt Gly tc t Ser cag Gin 190 ctt Leu ctt Leu gcg Ala gag Giu tca Ser ggc Gly t tc Phe gca Ala 95 gtg Val1 cct Pro gac

ASP

gct Ala ga t Asp 175 ttt Phe gaa Glu gat Asp cat His caa gct Ala gg t Gly aca Thr gcc Ala 80 gac Asp tgg Trp, gca Ala gcg Ala ga t Asp 160 ctt Leu ctt Leu gcg Ala cga Arg cct Pro 240 cat gcc Ala t tg Leu gtt Val1 agg Arg aat Asn gtg Val ata Ile act Thr 145 att Ile ctt Leu gcc Ala ttt Phe gca Ala 225 tcg Ser gaa atg Met gca Ala gtt Val atg Met' ac c Thr gaa Glu gtc Val 130 ttc Phe g tg Val1 ctt Leu act Thr ctt Leu 210 gaa.

Glu gtt Val aaa gga Gly gcg Ala t ta Leu gaa Glu gaa Glu tcg Ser 115 gac Asp gca Ala ctt Leu gga Gly cac His 195 gc t Ala tcc Ser acc Thr gcc PCTIIBOO/00923 Gly Arg act cta 211 Thr Leu gca acg 259 Ala Thr cct aaa 307 Pro Lys gcc cgc 355 Ala Arg gaa gtg 403 Glu Val 100 atc gct 451 Ile Ala ggt gtg 499 Gly Val acg cca 547 Thr Pro tac tcg 595 Tyr Ser 165 gtc gca 643 Val Ala 180 cgrt cat 691 Arg His ctc cgt 739 Leu Arg aac gca 787 Asn Ala cgc gtc 835 Arg Val 245 gtg cga 883 Val Arg aat tat cca gga ctt cct gat gat ccc Asn Tyr Pro Gly Leu Pro Asp Asp Pro Gin His Giu Lys Ala WO 01/00843 WO 0100843PCTIBOO/00923 gtc cta Val Leu aca cct Thr Pro cac gcg 1.027 His Ala 295 agg cgg 1075 Arg Arg 310 gtt tcc 1123 Val Ser gcc tca 1170 Ala Ser ccc tct Pro Ser 265 gaa cga Glu Arg 280 acc agt Thr Ser gat gct Asp Ala gta gga Val Gly atc gac Ile Asp 345 250 gga Gly act Thr tgg Trp gaa Glu att Ile 330 aaa Lys tgt gga aac Cys Gly Asn 255 260 atg ttg tca ttt gag ctt gat gca Met Leu Ser Phe Glu Leu Asp Ala 270 275 ctc gaa agc ctg tca ctt tta acc Leu Glu Ser Leu Ser Leu Leu Thr 290 gaa aca gcc att gaa cgt cgc acc Glu Thr Ala Ile Glu Arg Arg Thr 305 gaa gta ccg atg act ctt tgc cgc Glu Val Pro Met Thr Leu Cys Arg 320 325 gaa gat cta tgg gaa gac ctc aac Glu Asp Leu Trp Glu Asp Leu Asn 335 340 tagaactcgt agccagtaac cag <210> 196 <211> 349 <212> PRT <213> Corynebacteritun glutamicum <400> 196 Met Asri Pro Pro Ile Thr Leu Ser Ser 1 Lys Ala Tyr Gly Arg Asp Gly Asn Asp Ala Met Gly Thr Leu Asp Gly Gly Phe 40 Leu Ala Ala Ala Thr Ser Ile Ala Asp 55 Val Val Leu Pro Lys Ala Ala Tyr Tyr 70 Arg Met Glu Ala Arg Gly Arg Leu Lys Asn Thr Glu Glu Val Ile Ala Ala Ala 100 105 Val Glu Ser Ile Ala Asn Pro Thr Met 115 120 Tyr Trp, Val1 Val1 Val1 Arg Gly Val1 Val Gly Ser Pro Thr Thr Ala Ala His Ala Tyr Thr Asn Val1 Asp Asp 125 WO 01/00843 Ile Val Asp 130 Thr Phe Ala 145 Ile Val Leu Leu Leu Gly Ala Thr His 195 Phe Leu Ala 210 Ala Giu Ser 225 Ser Val Thr Glu Lys Ala Phe Glu Leu 275 Leu Ser Leu 290 Ilie Glu Arg 305 Met Thr Leu Trp Glu Asp PCT/1EB00100923 Gly Thr Tyr Val1 180 Arg Leu Asn Arg Val1 260 Asp Leu Arg Cys Leu 340 Val1 Pro Ser 165 Ala His Arg Ala Val 245 Arg Ala Thr Thr Arg 325 Asn Leu Gin Lys Lys Gly 200 Tyr Leu Pro Pro Glu 280 Thr Asp Val Ile Val Leu His Ala Gly 205 Arg Asn Asp Asn Ile 285 Val1 Ala Val Gly <210> 197 <211> 861 <212> DNA <213> Corynebacteriun glutanicum <2 <221> CDS <222> (838) <223> RXS03159 <400> 197 aggggctagt tttacacaaa agtggacagc ttggtctatc attgccagaa gaccggtcct tttagggcca tagaattctg attacaggag ttgatctacc ttg tct ttt gac cca 115 Leu Ser Phe Asp Pro 1 aac acc cag ggt ttc tcc act gca tcg att cac gct ggg tat gag cca 163 286 WO 01/00843 Asn Thr GI gac gac ta Asp Asp T ttc gcg ca Phe Ala GI 4 cgt: gtg gG Arg Val GI ctc gaa gq Leu Glu GI acc gac at Thr Asp I] ctc ggc aa Leu Gly AE ttc acc gc Phe Thr A] 12 gaa. gag gt Glu Glu Ve 135 gaa acc cc Glu Thr Pi 150 gca aag ct Ala Lys LE ttg gca tc Leu Ala SE agt cct tq Ser Pro Cy 2 C ttg gcg gc Leu Ala Al 215 tgt tca tq Cys Ser Cy Phe gg t Gly gct Ala ccc Pro aag Lys t tc Phe gct Ala ggc Gly gca Ala aac Asn gaa Glu 170 tac Tyr cca Pro tgg Trp gcg Ser tcg S er cca Pro acc Thr tat Tyr 75 cgc Arg tac Tyr gtc Val1 gcg Ala cca Pro 155 ggc Gly ctg Leu cca Pro gta Val gca Ser acc Thr ctg Leu gca Ala gca Ala ctc Leu acc Thr 110 acc Thr gac Asp ggc Gly gcc Ala cca Pro 190 aca Thr acc Thr cga Arg Ala tat Tyr ggC Gly cag Gin tcC Ser ggc Gly ctg Leu gat Asp aag Lys 145 gac Asp gt t Val1 ctc Leu gac Asp tgg Trp 225 cag Gin Gly gcc Ala tac Tyr acc Thr ggc Gly gat Asp atc Ile acc Thr 130 c tg Leu atc Ile gtt Val1 Gly act Thr 210 acg Thr ttt Phe Tyr tcc Ser gag Glu gtc Val atg Met cac Hius gac Asp 115 tcC Ser atc Ile gaa Glu gac Asp gca Al a 195 ccg Pro aag Lys tcg Ser PCT/1IB00I00923 Glu Pro acc acc 211 Thr Thr tac acc 259 Tyr Thr gca gca 307 Ala Ala gct gca 355 Ala Ala atc gtc 403 Ile Val 100 acc gta 451 Thr Val gtc gtg 499 Val Val tgg gtg 547 Trp Val gca gta 595 Ala Val 165 aac acc 643 Asn Thr 180 cac gca 691 His Ala acg ttg 739 Thr Leu aac tgc 787 Asn Cys atg cat 835 Met His Arg Ala Ala Ser Asp 235 acc Thr tgaccgcccg tggcctcaag acc WO 01/00843 WO 0100843PCTA~BOOIOO923 <210> 198 <211> 246 <212> PRT <213> Corynebacteriun glutamicumr <400> 198 Leu Ser Phe Asp Pro Asn Thr Gin Gly 1 Ala Tyr Gly Gin Ser Gly Leu Asp Lys 145 Asp Val1 Leu Asp Trp 225 Gin Giu Thr Tyr Ala Ala Ile 100 Thr Val1 Trp Aia Asn 180 His Thr Asn Met 5 Pro Thr Thr Ala Ala Val Val Vali Val Val1 165 Thr Ala Leu Cys His 245 Asp Phe Arg Leu 70 Thr Leu Phe Giu Glu 150 Ala Leu Ser Leu Cys 230 Thr Phe Gly Ala Pro Lys Phe Ala Gly Ala Asn Glu 170 Tyr Pro Trp Ser Thr Ala Ser Pro Thr Tyr 75 Arg Tyr Val1 Ala Pro 155 Gly Leu Pro Val1 Ala 235 Ile Asn Ile Gly Ile Gly Glu Ile 140 Ala Thr Gin Pro Pro 220 Ser His Ile Lys Giu Ser Pro Arg Val Thr Thr 160 Leu Lys Lys Lys His 240 Cys Arg Ala <210> 199 <211> 703 <212> DNA WO 01/00843 WO 0100843PCTIBOOIOO923 <213> Corynebacteriun giutamicun <220> <221> CDS <222> (101)..(703) <223> FRXA02768 <400> 199 aggggctagt tttacacaaa agtggacagc ttggtctatc tttagggcca tagaattctg attacaggag ttgatctacc attgccagaa gaccggtcct ttg tct ttt gac cca ii! Leu Ser Phe Asp Pro aac acc cag ggt Asn Thr Gin Gly t tc Phe tcc act gca tcg Ser Thr Ala Ser cac gct ggg tat His Ala Gly Tyr gag cca Giu Pro gac gac tac Asp Asp Tyr ttc gcg cag Phe Ala Gin tac Tyr ggt tcg att aac acc cca atc tat gcc Gly Ser Ile Asn Thr Pro Ile Tyr Ala tcc acc acc Ser Thr Thr gag tac acc Giu Tyr Thr aac gct cca aac gaa ctg cgc aaa ggc Asn Ala Pro Asn Giu Leu Arg Lys Gly t ac Tyr cgt gtg Arg Val ggc aac ccc acc Giy Asn Pro Thr gtg gca tta gag Val Ala Leu Giu cag Gin acc gtc gca gca Thr Val Ala Ala ctc Leu gaa ggc gca aag Glu Giy Ala Lys ggc cgc gca ttc Gly Arg Ala Phe tcc ggc atg gct Ser Gly Met Ala acc gac atc ctg Thr Asp Ilie Leu cgc atc atc ctc Arg Ile Ile Leu ccg ggc gat cac Pro Gly Asp His atc gtc Ile Vai 100 ctc ggc aac Leu Gly Asn ttc acc gca Phe Thr Ala 120 gat Asp 105 gct tac ggc gga Ala Tyr Gly Gly tac cgc ctg atc Tyr Arg Leu Ile gac acc gta Asp Thr Val 115 tcc gtc gtg Ser Val Val tgg ggc gtc gaa Trp Gly Val Giu acc gtt gtt gat Thr Val Val Asp acc Thr 130 gaa gag Giu Giu 135 gtc aag gca gcg Val Lys Ala Ala aag gac aac acc Lys Asp Asn Thr aag Lys 145 gct gat ctt ggt Ala Asp Leu Gly gga Gly 150 aac ccc aac caa Asn Pro Asn Gin agc act ttg gca Ser Thr Leu Ala tta Leu 160 ccc gac atc gaa Pro Asp Ile Giu gtn tgc aaa act Val Cys Lys Thr ccc gaa agg cac Pro Giu Arg His ccc caa gct tgt Pro Gin Ala Cys tgt ttg Cys Leu 180 aca aca cct Thr Thr Pro cat tcc cca tac His Ser Pro Tyr cag can cca ctt Gin Xaa Pro Leu aaa ant tnn Lys Xaa Xaa 195 WO 01/00843 WO 0100843PCTIiBOO/00923 gng cac acg cag 703 Xaa His Thr Gin 200 <210> 200 <211> 201 <212> PRT <213> Corynebacterium giutamicum <400> 200 Leu Ser Phe Asp Pro Asn Thr Gin Gly 1 Ala Tyr Gly Gin S er Gly Leu Asp Lys 145 Pro Gin Pro Asp Ala Val1 Glu Asp Gly Thr Glu 135 Asn Cys Thr His Tyr Gin Gly Gly Ile Asn Ala 120 Val1 Pro Lys Pro Thr 200 Phe 10 Gly Ala Pro Lys Phe Ala Gly Ala Gin Ser 170 His Ile Pro Arg Leu Phe Lys Tyr Val Asn Ala Gin 175 Gin <210> 201 <211> 1113 <212> DNA <213> Corynebacterium giutanicun <22 0> <221> CDS <222> (1090) <223> RXA00216 WO 01/00843 WO 0100843PCTIBOO/00923 <400> 201 gtgttgctcg cggccaggca gcagtgctgt acctgcctga cgcggatggt gacatcgttc ttggatcagg caccatctgc cacacggagt cttaagaaaa ttg ggc gct tat ggt tta Leu cag Gin ggt Gly atc Ilie agc Ser gcg Ala gtt Val1 cat Hius ctg Leu cgc Arg 150 atc Ile gtg Vai att Ilie tcc Ser c tc Leu ggt Gly cga Arg ggc Gly acg Thr ggt Gly ggt Gly 125 cat His att Ile act Thr gaa Giu aac Asn 205 gcc Ala cat His cga Arg tct Ser gat Asp ggc Giy gcg Ala 110 gcg Ala gca Ala gat Asp tcc Ser cgt Arg 190 ctc Leu Leu Gly Ala Tyr 1 gcc gcc gac att Ala Ala Asp Ile cag ctt ccg gcg Gin Leu Pro Ala ggt ctg ctg gac Gly Leu Leu Asp atg agc aca cgc Met Ser Thr Arg gac atg gat ttg Asp Met Asp Leu aag cta aaa atc Lys Leu Lys Ile 100 gag ttg gcc aat Giu Leu Ala Asn 115 gat ctc acg cag Asp Leu Thr Gin 130 gtt gct ggg cga Val Aia Gly Arg 145 gag ctg aaa tcg Giu Leu Lys Ser gac att att cct Asp Ile Ile Pro 180 cag gtc ttt agc Gin Val Phe Ser 195 cag att cct act Gin Ile Pro Thr 210 Gly att Ile cga Arg.

atg Met ccc Pro gat Asp caa Gin ggc Gly gcg Ala ttt Phe ctt Leu 165 gcg Al a tcg Ser tgg Trp 115 163 211 259 307 355 403 451 499 547 595 643 691 739 787 gat gtg gct cgg ggt gcg ggc gcc gat act gtg cag att tcc atg gat WO 01/00843 Asp Val Ala 215 caa gtc cgt Gin Val Arg 230 agt gga att Ser Gly Ile gat gaa ctg Asp Glu Leu ttt ttt gat Phe Phe Asp 280 gat att cat 1027 Asp Ile His 295 gcg cag gct 1075 Ala Gin Ala 310 gat tca tgc 1113 Asp Ser Cys Gly Ala aat gaa Asn Giu 235 ctt ggt Leu Gly 250 gag cga Glu Arg tta ggt Leu Gly ggt gag Gly Giu cgc atg Arg Met 315 ctt taac Leu 330 Giy Ala Asp Thr Val Gin Ile Ser 220 225 cat ttg gat ggt ttt ggt gaa acc His Leu Asp Giy Phe Gly Giu Thr 240 ttg ggc att acg aca gga aaa gat Leu Gly Ile Thr Thr Giy Lys Asp 255 ccg cgg caa aag gcc gtt gag gta Pro Arg Gin Lys Ala Val Giu Val 270 275 gtg ggc cga aac tat ctc gtg gat Val Gly Arg Asn Tyr Leu Vai Asp 285 290 gat ttg gtg cag ggg acc atc acc Asp Leu Vai Gin Gly Thr Ile Thr 300 305 gcc cgg gtg atg tcg gag atg ttg Ala Arg Val Met Ser Glu Met Leu 320 PCTLBOO/00923 Met Asp atc acc 835 Ile Thr 245 gtc gta 883 Val Val 260 gca cgc 931 Ala Arg gct gtt 979 Ala Val gag gcc Glu Ala tcg aag Ser Lys 325 jgcttta ccggcgctgg gtg <210> 202 <211> 330 <212> PRT <213> Corynebacteriun glutainicum <400> 202 Leu Giy Ala Tyr Gly Leu Gly Glu Leu 1 5 Ala Ala Asp Ile Ilie Gin Gly Giu Thr Gin Leu Pro Ala Arg Gly Leu Gly Ala Gly Leu Leu Asp Met Ile Asn Val Asp met Ser Thr Arg Pro Ser Arg Leu Thr 70 Asp Met Asp Leu Asp Ala Cys Giu Glu Lys Leu Lys Ile Gln Val Ala Gly Pro 100 105 Ala Ile Thr Trp Phe Val1 Arg Glu Pro Val1 Val1 Leu Asp Ile WO 01/00843 PCT/IB00/00923 Glu Leu Ala Asn Gly His Arg Val Leu Ser Asp Arg Gly Ala Met Arg 115 120 125 Asp Leu Thr Gin Ala Leu Ile Ala Gly Ile Asp Ala His Ala Arg Lys 130 135 140 Val Ala Gly Arg Phe Arg Ala Glu Val Gin Val Gin Ile Asp Glu Pro 145 150 155 160 Glu Leu Lys Ser Leu Ile Asp Gly Ser Leu Pro Gly Thr Ser Thr Phe 165 170 175 Asp Ile Ile Pro Ala Val Asn Val Ala Asp Ala Ser Glu Arg Leu Gin 180 185 190 Gin Val Phe Ser Ser Ile Glu Gly Pro Thr Tyr Leu Asn Leu Thr Gly 195 200 205 Gin Ile Pro Thr Trp Asp Val Ala Arg Gly Ala Gly Ala Asp Thr Val 210 215 220 Gin Ile Ser Met Asp Gin Val Arg Gly Asn Glu His Leu Asp Gly Phe 225 230 235 240 Gly Glu Thr Ile Thr Ser Gly Ile Arg Leu Gly Leu Gly Ile Thr Thr 245 250 255 Gly Lys Asp Val Val Asp Glu Leu Leu Glu Arg Pro Arg Gin Lys Ala 260 265 270 Val Glu Val Ala Arg Phe Phe Asp Arg Leu Gly Val Gly Arg Asn Tyr 275 280 285 Leu Val Asp Ala Val Asp Ile His Pro Gly Glu Asp Leu Val Gin Gly 290 295 300 Thr Ile Thr Glu Ala Ala Gin Ala Tyr Arg Met Ala Arg Val Met Ser 305 310 315 320 Glu Met Leu Ser Lys Asp Ser Cys Asp Leu 325 330 <210> 203 <211> 623 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> <223> RXN00402 <400> 203 act gac gaa aag gat gga aag cca gta ttg ccc tac ttc gtc act cca 48 Thr Asp Glu Lys Asp Gly Lys Pro Val Leu Pro Tyr Phe Val Thr Pro 1 5 10 gat gct get tac cac gga ttg aag tac gca gac ctt ggt gca cca gcc 96 Asp Ala Ala Tyr His Gly Leu Lys Tyr Ala Asp Leu Gly Ala Pro Ala 293 WO 01/00843 WO 0100843PCTIiBOO/00923 t tc Phe c tc Leu tcC Ser ttC Phe aag Lys acc Thr tgg Trp ggc Giy 145 cag Gin gtc Val1 gtt Val tgg Trp, cac His aag Lys gca Ala ttc Phe ctg Leu 135 gtt Val c tg Leu atc Ile ggc ctt cta cgc Gly Leu Leu Arg gct gca gtc cag Ala Ala Val Gin aac gaa aac gcc Asn Giu Asn Ala 75 gtg gaa aag gtt Val Glu LYS Vai acc aag gaa aag Thr Lys Giu Lys 105 gag atc aag ggc Giu Ilie Lys Gly 120 aag cta cac tcc Lys Leu His Ser gtt cac cca gca Val His Pro Ala 155 gca cgc gcg ggc Ala Arg Aia Gly 170 gag acc att gat Glu Thr Ile Asp 185 aco ggc tcc Thr Gly Ser atc gac acc Ile Asp Thr aag gtt gca Lys Val Ala ttc gca ggc Phe Ala Gly ggc ctg aag Gly Leu Lys 110 aag gat gag Lys Asp Giu 125 ctt gca aac Leu Ala Asn acc acc cat Thr Thr His acc cag tcc Thr Gin Ser 175 atc atc gct Ile Ile Ala 190 ac c Thr ctt Leu gaa Glu ctg Leu tac Tyr gct Ala atc Ile tca Ser 160 acc Thr gac Asp ctc gaa ggc ggc ttt gct gca atc tagctttaaa tagactcacc cca.

Leu Giu Gly Gly Phe Ala Ala Ile 195 200 <210> 204 <211> 200 <212> PRT <213> Corynebacterium glutamicuff <400> 204 Thr Asp Glu Lys Asp Gly Lys Pro 1 5 Asp Ala Ala Tyr His Gly Leu Lys Phe Gly Leu Lys Val Arg Val Gly 40 Val Leu Pro Tyr Phe Val Thr Pro 10 Tyr Ala Asp Leu Gly Ala Pro Ala 25 Leu Leu Arg Asp Thr Gly Ser Thr WO 01/00843 WO 0100843PCT/IB00100923 Leu Ser Ala Phe Asn Ala Ti-p Ala Ala Val Gin Gly Ile Asp Thr Leu 55 Ser Leu Arg Leu Glu Arg His Asn Glu Asn Ala Ile Lys Val Ala Giu 70 75 Phe Leu Asn Asn His Glu Lys Val Glu Lys Val Asn Phe Ala Gly Leu 90 Lys Asp Ser Pro Trp Tyr Ala Thr Lys Glu Lys Leu Gly Leu Lys Tyr 100 105 110 Thr Gly Ser Val Leu Thi- Phe Glu Ile Lys Gly Gly Lys ASP Giu Ala 115 120 125 Ti-p Ala Phe Ile Asp Ala Leu Lys Leu His Ser Asn Leu Ala Asn Ile 130 135 140 Gly Asp Val Arg Ser Leu Val Val His Pro Ala Thr Thr Thi- His Ser 145 150 155 160 Gin Ser Asp Giu Ala Gly Leu Ala Arg Ala Gly Val Thr Gin Ser Thi- 165 170 175 Vai Arg Leu Ser Val Gly Ilie Glu Th- Ile Asp ASP Ilie Ile Ala Asp 180 185 190 Leu Giu Gly Gly Phe Ala Ala Ile 195 200 <210> 205 <211> 599 <212> DNA <213> Corynebacterium giutainicum <220> <221> CDS <222> <223> FRXA00402 <400> 205 gta ttg ccc tac ttc gtc act cca gat gct gct tac cac gga ttg aag 48 Val Leu Pro Tyr Phe Val Thr Pro Asp Ala Ala Tyr His Gly Leu Lys 1 5 10 tac gca gac ctt ggt gca cca gcc ttc ggc ctc aag gtt cgc gtt ggc 96 Tyr Ala Asp Leu Gly Ala Pro Ala Phe Gly Leu Lys Val Arg Val Gly 25 ctt cta cgc gac acc ggc tcc acc ctc tcc gca ttc aac gca tgg gct 144 Leu Leu Arg Asp Thr Gly Ser Thr Leu Ser Ala Phe Asn Ala Ti-p Ala 40 gca gtc cag ggc atc gac acc ctt tcc ctg cgc ctg gag cgc cac aac 192 Ala Vai Gin Gly Ile Asp Thr Leu Ser Leu Arg Leu Giu Arg His Asn 55 gaa aac gcc atc aag gtt gca gaa ttc ctc aac aac cac gag aag gtg 240 Giu Asn Ala Ile Lys Val Ala Giu Phe Leu Asfl Asn His Giu Lys Vai 295 WO 01/00843 gaa Glu aag Lys atc Ile cta Leu cac His 145 cgc Arg acc 70 gca Ala ctg Leu gat Asp gca Ala acc Thr 150 cag Gin atc ggc Gly aag Lys gag Glu aac Asn 135 cat His tcc Ser gc t Ala tac Tyr acc Thr 110 gcc Ala c tc Leu ggc Gly ggc Gly gc t Ala PCTIBOO/00923 gca acc 288 Ala Thr ttc gag 336 Phe Glu ctg aag 384 Leu Lys gtt gtt 432 Val Val ctg gca 480 Leu Ala 160 atc gag 528 Ile Glu 175 gca atc 576 Ala Ile Thr Ile Asp Asp Ile Ilie 180 tagctttaaa tagactcacc cca <210> 206 <211> 192 <212> PRT <213> Corynebacterium giutamicum <400> 206 Val Leu Pro Tyr Phe Val Thr Pro Asp, 1 5 Tyr Ala Asp Leu Gly Ala Pro Ala Phe 25 Leu Leu Arg Asp Thr Gly Ser Thr Leu Ala Vai Gin Gly Ilie Asp Thr Leu Ser Glu Asn Ala Ile Lys Vai Ala Giu Phe Glu Lys Val Asn Phe Ala Gly Leu Lys Lys Giu Lys Leu Gly Leu Lys Tyr Thr, 100 105 Ile Lys Gly Gly Lys Asp Giu Ala Trp 296 Gly Arg Ala Arg Giu Tyr Thr 110 Ala Leu Val1 Trp His Lys Ala Phe Leu WO 01/00843 WO 0100843PCTJIBOO/00923 Leu His 145 Arg Thr 115 120 125 Ser Asn Leu Ala Asn Ile Gly Asp Val Arg Ser Leu Val 135 140 Ala Thr Thr Thr His Ser Gin Ser Asp Giu Ala Gly Leu 150 155 Giy Val Thr Gin Ser Thr Val Arg Leu Ser Val Gly Ile 165 170 175 Asp Asp Ile Ilie Ala Asp Leu Giu Gly Gly Phe Ala Ala 180 185 190 <210> 207 <211> 613 <212> DNA <213> Corynebacterium giutamicum <2 <221> CDS <222> (101)..(613) <223> RXA00405 <400> 207 agaataaatt tataccacac agtctattgc aatagaccaa gctgttcagt agggtgcatg ggagaagaat ttcctaataa aaactcttaa ggacctccaa atg cca aag tac Met Pro Lys Tyr aat Asn ggc Gly caa Gin t tc Phe acc Thr cac His t tg t99 Trp cag Gin ttc Phe ggc Gly aac Asn tcc Ser ggc ggC Gly acc Thr gac Asp cct Pro cgc Arg gga Gly gac att Ile ccg Pro aag Lys acc Thr gg t Gly aac Asn cca cac His a tc Ile cag Gin aac Asn ggc G ly gcc Ala 100 cgc Leu Asn Leu Ala Gly Ala Gly Asp His Ile Vai Thr Ser Pro Arg Leu WO 01/00843 WO 0100843PCTIiBOO/00923 tac Tiyr atc Ile gca Ala 150 aac Asn ggt Gly ga t Asp 135 gcc Ala cca Pro 105 ggc acc Gly Thr 120 gtt tcc Val Ser gtt cag Val Gin cag gca Gin Ala act Thr gtg Val aac Asn 155 gtc Val 110 115 cta ttc ctt atc act ctt aac cgc Ctg ggt Leu Phe Leu Ile Thr Leu Asn Arg Leu Gly 125 130 gaa aac ccc gac gac cct gag tcc tgg cag Glu Asn Pro Asp Asp Pro Giu Ser Trp Gin 140 145 acc aaa gca ttc ttc ggc gag act ttc gcc Thr Lys Ala Phe Phe Gly Glu Thr Phe Ala 160 165 170 <210> 208 <211> 171 <212> PRT <213> Corynebacterium glutamicum <400> 208 Met Pro Lys Tyr Asn Ser Asn Ala 1 Arg Asn His Arg Leu Thr Thr Leu Pro 145 Gly Ser Leu Ala Leu Giu Thr Ser Asn 130 Glu Glu Ile Pro Lys Thr Gly Asn Pro 115 Arg Ser Thr His Ile Gin Asn Gly Ala 100 Arg Leu Tx-p Gly Gin Phe Thr 70 His Leu Tyr Ile Ala 150 Gin Ser Al a 55 Val Al a Asn Gly Asp 135 Ala Asp Asp Phe Asp Leu Phe Gly Glu Phe Pro Gin Al a Val Leu Giu 75 Ser Ala Thr Val1 Asn 155 Trp Gin Phe Gly Asn Ser Gly Leu 6lu 140 Thr Thr Arg Giu Ser Ser Ala Val Thr Asp Phe 160 Phe Ala Asn Pro Gin Ala Asp Val <210> 209 <211> 551 WO 01/00843 PCTIiBOO/00923 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> <223> RXA02197 <400> 209 gcc gaa cgc atg cgc ttt agc ttc cca cgc cag cag cgc ggc agg ttc 48 Ala Giu Arg Met Arg Phe Ser Phe Pro Arg Gin Gin Arg Gly Arg Phe 1 5 10 ttg tgc atc gcg gat ttc att cgc cca cgc gag caa gct gtc aag gac 96 Leu Cys Ile Ala Asp Phe Ile Arg Pro Arg Giu Gin Ala Val Lys Asp 25 ggc caa gtg gac gtc atg cca ttc cag ctg gtc acc atg ggt aat cct 144 Giy Gin Val Asp Val Met Pro Phe Gin Leu Val Thr Met Gly Asn Pro 40 att gct gat ttc gcc aac gag ttg ttc gca gcc aat gaa tac cgc gag 192 Ile Ala Asp Phe Ala Asn Giu Leu Phe Ala Ala Asn Glu Tyr Arg Giu 55 tac ttg gaa gtt cac ggc atc ggc gtg cag ctc acc gaa gca ttg gcc 240 Tyr Leu Giu Val His Gly Ile Gly Vai Gin Leu Thr Giu Ala Leu Ala 70 75 gag tac tgg cac tcc cga gtg cgc agc gaa ctc aag ctg aac gac ggt 288 Giu Tyr Trp His Ser Arg Vai Arg Ser Giu Leu Lys Leu Asn Asp Gly 90 gga tct gtc gct gat ttt gat cca gaa gac aag acc aag ttc ttc gac 336 Gly Ser Val Ala Asp Phe Asp Pro Giu Asp Lys Thr Lys Phe Phe Asp 100 105 110 ctg gat tac cgc ggc gcc cgc ttc tcc ttt ggt tac ggt tct tgc cct 384 Leu Asp Tyr Arg Gly Ala Arg Phe Ser Phe Gly Tyr Gly Ser7 Cys Pro 115 120 125 gat ctg gaa gac cgc gca aag ctg gtg gaa ttg ctc gag cca ggc cgt 432 Asp Leu Giu Asp Arg Ala Lys Leu Val Giu Leu Leu Giu Pro Gly Arg 130 135 140 atc ggc gtg gag ttg tcc gag gaa ctc cag ctg cac cca gag cag tcc 480 Ile Gly Val Giu Leu Ser Giu Giu Leu Gin Leu His Pro Giu Gin Ser 145 150 155 160 aca gac gcg ttt gtg ctc tac cac cca gag gca aag tac ttt aac gtc 528 Thr Asp Ala Phe Val Leu Tyr His Pro Glu Ala Lye Tyr Phe Asn Val 165 170 175 taacaccttt gagagggaaa act 551 <210> 210 <211> 176 <212> PRT <213> Corynebacteriui glutamicun WO 01/00843 <400> 210 Ala Glu Arg 1 Leu Cys Ilie Gly Gin Val Ilie Ala Asp Tyr Leu Glu Glu Tyr Trp Gly Ser Val Leu Asp Tyr 115 Asp Leu Glu 130 Ile Gly Val 145 Thr Asp Ala PCT/BOOIOO923 Arg 5 Asp Val Ala His Ser Asp Gly Arg Leu Val 165 Phe Phe Met Asn Gly Arg Phe Ala Ala Ser 150 Leu Phe Arg Phe 40 Leu Gly Arg Pro Phe 120 Leu Glu His Arg Ala Met Glu Glu Leu Lys Gly 125 Glu Pro Tyr Gly Val Gly Tyr Ala Asn Phe 110 Ser Pro Glu Phe Arg Lys Asn Arg Leu Asp Phe Cys Gly Gin Asn 175 <210> 211 <211> 2599 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (101)..(2599) <223> RXN02198 <400> 211 agactagtgg cgctttgcct gtgttgctta ggcggcgttg aaaatgaact acgaatgaaa agttcgggaa ttgtctaatc cgtactaagc tgtctacaca atg tct act tca .gtt Met Ser Thr Ser Val 1 act tca cca gcc cac aac aac gca cat tcc tcc gaa ttt ttg gat gcg Thr Ser Pro Ala His Asn Asn Ala His Ser Ser Glu Phe Leu Asp Ala 15 ttg gca aac cat gtg ttg atc ggc gac ggc gcc atg ggc acc cag ctc Leu Ala Asn His Val Leu Ile Gly Asp Gly Ala Met Gly Thr Gin Leu 300 WO 01/00843 WO 0100843PCTIBOOIOO923 30 caa ggc ttt gac ctg gac gtg gaa aag gat ttc ctt gat ctg gag ggg 259 Gin Gly Phe Asp Leu Asp Val Giu Lys Asp Phe Leu Asp Leu Glu Gly 45 tgt aat gag att ctc aac gac acc cgc cct gat gtg ttg agg cag att 307 Cys Asn Giu Ilie Leu Asn Asp Thr Arg Pro Asp Val Leu Arg Gin Ile 60 cac cgc gcc tac ttt gag gcg gga gct gac ttg gtt gag acc aat act 355 Hius Arg Ala Tyr Phe Glu Ala Gly Ala Asp Leu Val Giu Thr Asn Thr 75 80 ttt ggt tgc aac ctg ccg aac ttg gcg gat tat gac atc gct gat cgt 403 Phe Gly Cys Asn Leu Pro Asn Leu Ala Asp Tyr Asp Ile Ala Asp Arg 95 100 tgc cgt gag ctt gcc tac aag ggc act gca gtg gct agg gaa gtg gct 451 Cys Arg Giu Leu Ala Tyr Lys Gly Thr Ala Val Ala Arg Giu Val Ala 105 110 115 gat gag atg ggg ccg ggc cga aac ggc atg cgg cgt ttc gtg gtt ggt 499 Asp Giu Met Gly Pro Gly Arg Asn Gly Met Arg Arg Phe Val Vai Gly 120 125 130 tcc ctg gga cct gga acg aag ctt cca tcg ctg ggc cat gca ccg tat 547 Ser Leu Giy Pro Gly Thr Lys Leu Pro Ser Leu Gly His Ala Pro Tyr 135 140 145 gca gat ttg cgt ggg cac tac aag gaa gca gcg ctt ggc atc atc gac 595 Ala Asp Leu Arg Gly His Tyr Lys Glu Ala Ala Leu Gly Ile Ile Asp 150 155 160 165 ggt ggt ggc gat gcc ttt ttg att gag act gct cag gac ttg ctt cag 643 Gly Giy Giy Asp Ala Phe Leu Ile Giu Thr Ala Gin Asp Leu Leu Gin 170 175 180 gtc aag gct gcg gtt cac ggc gtt caa gat gcc atg gct gaa ctt gat 691 Val Lys Ala Aia Val His Gly Vai Gin Asp Ala met Ala Giu Leu Asp 185 190 195 aca ttc ttg ccc att att tgc cac gtc acc gta gag acc acc ggc acc 739 Thr Phe Leu Pro Ile Ile Cys His Val Thr Val Giu Thr Thr Gly Thr 200 205 210 atg ctc atg ggt tct gag atc ggt gcc gcg ttg aca gcg ctg cag cca 787 Met Leu Met Giy Ser Glu Ile Gly Ala Ala Leu Thr Ala Leu Gin Pro 215 220 225 ctg ggt atc gac atg att ggt ctg aac tgc gcc acc ggc cca gat gag 835 Leu Gly Ile Asp Met Ilie Gly Leu Asn Cys Ala Thr Gly Pro Asp Glu 230 235 240 245 atg agc gag cac ctg cgt tac ctg tcc aag cac gcc gat att cct gtg 883 Met Ser Glu His Leu Arg Tyr Leu Ser Lys His Ala Asp Ile Pro Val 250 255 260 tcg gtg atg cct aac gca ggt ctt cct gtc ctg ggt aaa aac ggt gca 931 Ser Val Met Pro Asn Aia Gly Leu Pro Val Leu Gly Lys Asn Gly Ala 265 270 275 WO 01/00843 WO 0100843PCT/IB00100923 gaa tac cca ctt Glu Tyr Pro Leu 280 gag gct gag gat ttg gcg cag gcg ctg gct gga ttc 979 Gly Phe gtc tcc 1027 Val Ser 295 cct gag 1075 Pro Giu 310 cag gaa 1123 Gin Giu gcc tcc 1171 Ala Ser tcg gtg 1219 Ser Val acc aac 1267 Thr Asn 375 gat tgg 1315 Asp Trp 390 gca cac 1363 Ala His gcc gat 1411 Ala Asp cca atc 1459 Pro Ile gag cac 1507 Glu His 455 gaa tat Glu Tyr Glu Ala Giu ggc ctg tcc Gly Leu Ser 300 Leu Ala Gin Ala Leu Ala gtg ggt ggt tgt Val Gly Gly Cys 305 gat gcg gtg gtt Asp Ala Val Val 320 290 tgt ggc acc aca Cys Gly Thr Thr ggt gtt cca gag Gly Val Pro Giu 325 cac atc cgt gcg gtc cgc His Ile Arg Ala Val Arg 315 acc tcc aca ctg acc aag Thr Ser Thr Leu Thr Lys atc cct Ile Pro cgc gag Arg Giu 345 cca ttg Pro Leu 330 gtg Val1 tcc Ser gag aaa gag gac Giu Lys Giu Asp 350 cag gaa acc ggc Gin Giu Thr Gly tcc Ser att Ile 335 gca ggc cct gtt gag cag Ala Gly Pro Val Glu Gin 340 gtc gcg tcg ctg tac acc Val Ala Ser Leu Tyr Thr 355 tcc atg atc ggt gag cgc Ser Met Ile Gly Glu Arg 360 tcc Ser 365 aac ggt tcc aag Asn Gly Ser Lys 380 gca Ala ttc Phe 370 cgt gag gca atg ctg tct ggc Arg Glu Ala Met Leu Ser Gly 385 gaa aag tgt gtg Glu Lys Cys Val 395 atg ctg gat ctt Met Leu Asp Leu gat att gcc aag Asp Ile Ala Lys tgt gtg gat tac Cys Val Asp Tyr 415 gca gca ctt ctt Ala Ala Leu Leu cag Gin 400 caa Gin acc cgc gat ggt Thr Arg Asp Gly 405 atg gcg Met Ala 425 atg att Met Ilie 440 410 acc Thr ttg Leu gtg gga cga gac ggc acc Val Gly Arg Asp Gly Thr 420 gct acc agc tcc act ttg Ala Thr Ser Ser Thr Leu 435 gtt att cgc aca ggc ctt Val Ile Arg Thr Gly Leu 430 gac tcc acc gag Asp Ser Thr Giu 445 gga cga agc atc Gly Arg Ser Ilie 460 cca Pro gag Giu ttg ggt Leu Gly 450 gtt aac tcc gtc aac ttt gaa gac Val Asn Ser Val Asn Phe Giu Asp 465 WO 01/00843 WO 0100843PCTJIBOO/00923 ggc gat 1555 Gly Asp 470 cag cac 1603 Gin His gca cgt 1651 Ala Arg gat atc 1699 Asp Ile ggc cct Gly Pro ggt gcg Gly Ala acc gct Thr Ala 505 acc ggc Thr Gly gag tcc Glu Ser 475 cgc tac cag cgc atc Arg Tyr Gin Arg Ile 480 gcc gtg gtt gcg ctg acc Ala Val Val Ala Leu Thr 490 495 gag cac aag gtg cgc att Giu His Lys Val Arg Ile 510 agc tac ggc ctg gat atc Ser Tyr Gly Leu Asp Ile 525 ccg atc tct act ggc cag Pro Ile Ser Thr Gly Gin att Ile gc t Ala atg aaa ctg gta aag Met LYS Leu Val Lys 485 gat gag gaa ggc cag Asp Giu Giu Gly Gin 500 aaa cga ctg att gac Lys Arg Leu Ile Asp 515 aaa gac atc gtt gtg gac Lys Asp Ile Val Val Asp 530 gaa gaa acc agg cga gat Giu Glu Thr Arg Arg Asp tgc ctg acc 1747 Cys Leu Thr 535 ggc att gaa 1795 Giy Ile Giu 550 gaa atc cac 1843 Giu Ile His t tc Phe acc atc gaa Thr Ile Glu 540 gcc atc cgc gag Ala Ile Arg Giu ggt ctg tcc aat Gly Leu Ser Asn 575 545 ctg aag Leu Lys 560 att tcc Ile Ser aag ctc tac cca Lys Leu Tyr Pro 565 ttc ggc ctg aac Phe Giy Leu Asn 580 acc acc Thr Thr 570 cct gct 1891 Pro Ala gag gct 1939 Giu Ala atg aac 1987 Met Asn 615 tat gat 2035 Tyr Asp 630 ctg ttt 2083 Leu Phe gca cgc Ala Arg 585 ggt ctg Gly Leu cag gtt ctt aac tct Gin Val Leu Asn Ser 590 gac tct gcg att gcg Asp Ser Ala Ile Ala 605 gat gat cgc cag cgc Asp Asp Arg Gin Arg gtg ttc ctc aat gag tgc att Val Phe Leu Asn Giu Cys Ile 595 cac agc tcc aag att ttg ccg His Ser Ser Lys Ile Leu Pro 610 gaa gtg gcg ttg gat atg gtc Giu Val Aia Leu Asp Met Val 600 cgc Arg att Ile 620 625 cgc cgc acc gag Arg Arg Thr Glu 635 gag ggc gtt tct Glu Gly Val Ser 650 gat tac gat ccg ctg Asp Tyr Asp Pro Leu 640 gct gcc gat gcc aag Ala Ala Asp Ala Lys 655 cag gaa ttc atg cag Gin Glu Phe Met Gin 645 gat gct cgc gct gaa Asp Ala Arg Ala Giu 660 WO 01/00843 WO 0100843PCTfiBOOIO0923 cag ctg 2131 Gin Leu gac ggc 2179 Asp Gly gag aag 2227 Giu Lys 695 aag acc 2275 Lys Thr 710 gtg ctg 2323 Val1 Leu ccg ttc 2371 Pro Phe ggc aag 2419 Gly Lys atc ggc 2467 Ilie Gly 775 gtg gtg 2515 Val Vai 790 gcg gaa 2563 Ala Giu aag tcc 2599 Lys Ser gcc gct Ala Aia 665 gat aag Asp Lys 680 tct cct Ser Pro atg cet ttg ttt gag Met Pro Leu Phe Giu 670 aat ggc ett gag gat Asn Gly Leu Giu Asp 685 att gcg atc atc aac Ile Ala Ile Ile Asn cgt ttg gca cag cgc atc atc Arg Leu Ala Gin Arg Ile Ile 675 gat ctg gaa gca ggc atg aag Asp Leu Giu Ala Gly Met Lys 690 gag gac ctt ctc aac ggc atg Giu Asp Leu Leu Asn Gly Met gtg ggt gag ctg Val Giy Giu Leu 715 caa tcg gea gaa Gin Ser Ala Giu 705 ttt ggt tcc gga eag Phe Gly Ser Gly Gin 720 ace atg aaa act gcg Thr Met Lys Thr Ala 735 atg cag ctg cca ttc Met Gin Leu Pro Phe 725 gtg gec tat ttg gaa Val Ala Tyr Leu Giu 740 atg gaa Met Giu 745 ggc aaa Gly Lys gaa gca gaa gct Giu Ala Giu Ala 750 gtc gtg gcc acc Vai Val Ala Thr ate Ile acc gga tct gcg cag gca gag Thr Gly Ser Ala Gin Ala Giu 755 gtc aag ggt gac gtg cac gat Val Lys Gly Asp Vai His Asp 770 ttg tcc aac aac ggt tac gac Leu Ser Asn Asn Giy Tyr Asp aac ttg gtg 765 gac atc att Asp Ile Ile Asn Leu Val aac ttg ggc ate Asn Leu Gly Ile 795 gaa cac aaa gca Glu His Lye Ala 810 ace gtg gtg atg Thr Val Val Met 825 780 785 aag cag cca ctg tcc gcc Lye Gin Pro Leu Ser Ala 800 atg ttg gaa gca Met Leu Giu Ala 805 gga ctt ctt gtg Gly Leu Leu Val 820 gac gtc atc ggc Asp Val Ile Gly 815 atg tcg Met Ser aag caa acc ate age gac Lys Gin Thr Ile Ser Asp 830 <210> 212 <211> 833 <212> PRT <213> Corynebacterium giutamicum WO 01/00843 <400> 212 Met Ser Thr 1.

Giu Phe Leu Met Gly Thr Leu Asp Leu Val Leu Arg Val Giu Thr Asp Ile Ala Ala Arg Glu 115 Arg Phe Val 130 Gly His Ala 145 Leu Gly Ile Gin Asp Leu Met Ala Giu 195 Giu Thr Thr 210 Thr Ala Leu 225 Thr Giy Pro Ala Asp Ile Gly Lys Asn 275 Ala Leu Ala 290 Cys Cys Giy 305 PCTIBOO/00923 Ser Asp Gin Giu Gin Asn Asp 100 Val1 Val Pro Ile Leu 180 Leu Giy Gin Asp Pro 260 Giy Gly Thr Val1 5 Ala Leu Gly Ile Thr Arg Ala Gly Tyr Asp 165 Gin Asp Thr Pro Giu 245 Val1 Aia Phe Thr Thr Leu Gin Cys His Phe Cys Asp Ser Ala 150 Gly Val1 Thr Met Leu 230 Met Ser Glu Vali Pro 310 Pro Asn Phe 40 Giu Ala Cys Giu Met 120 Gly Leu Giy Ala Leu 200 Met Ile Giu Met Pro 280 Giu His Ala Giy Giu Thr Giy Leu Gly Asn 125 Leu Lys Ile Vali His 205 Gly Leu Leu Leu Asp 285 Met Arg His Asp Lys Arg Ala Al a Thr .110 Gly Pro Giu Giu Gin 190 Val Ala* Asn Ser Pro 270 Leu Val1 Asp Ser Gly Asp Pro Asp Asp Ala Met Ser Ala Thr 175 Asp Thr Ala Cys Lys 255 Val Ala Gly Ala WO 01/00843 Val Gly Val Gly Pro Vai Ala Ser Leu 355 Met Ile Gly 370 Ala Met Leu 385 Gin Thr Arg Gly Arg Asp Thr Ser Ser 435 Ile Arg Thr 450 Vai Asn Phe 465 Met Lys Leu Asp Giu Giu Lys Arg Leu 515 Asp Ile Val 530 Giu Thr Arg 545 Lys Lys Leu Ser Phe Gly Leu Asn Giu 595 Ser Lys Ile 610 Ala Leu Asp 625 Pro Giu 340 Tyr Giu Ser Asp Gly 420 Thr Giy Glu Val Gly 500 Ile Val1 Arg Tyr Leu 580 Cys Leu Met Giu 325 Gin Thr Arg Gly Gly 405 Thr Leu Leu Asp Lys 485 Gin Asp Asp Asp Pro 565 Asn Ile Pro Vali Giu Ser Val1 Asn 375 Trp His Asp Ile His 455 Asp His Arg Ile Leu 535 Ile Ile Aia Ala Asn 615 Asp Leu Giu Gin Ser Val 395 Leu Leu Ser Arg Ser 475 Val His Tyr Ile Giu 555 Leu Vali Ser Asp Glu 635 Thr Lys Oiu Lys 380 Asp Cys Aia Thr Ser 460 Arg Val1 Lys Gly Ser 540 Al a Gly Leu Ala Arg 620 Asp Lys Giu Thr 365 Ala Ile Val Aia Giu 445 Ile Tyr Ala Val Leu 525 Thr Ile Leu Asn Ile 605 Gin Tyr PCTIIBOO/00923 Pro Ala 335 Ser Val Ile Ser Arg Giu Lys Gin 400 Tyr Val 415 Leu Ala Giu Val Asn Ser Arg Ile 480 Thr Ile 495 Ile Ala Ile Lys Gin Giu Giu Leu 560 Asn Ilie 575 Val Phe His Ser Giu Val Pro Leu 640 Gin Giu Phe Met Gin Leu Phe Giu Gly Val Ser Ala Ala Asp Ala Lys WO 01/00843 WO 0100843PCTIBOO/00923 645 650 655 Asp Ala Arg Ala Giu Gin Leu Ala Ala Met Pro Leu Phe Giu Arg Leu 660 665 670 Ala Gin Arg Ile Ile Asp Gly Asp Lys Asn Gly Leu Glu Asp Asp Leu 675 680 685 Giu Ala Gly Met Lys Giu Lys Ser Pro Ile Ala Ile Ilie Asn Giu Asp 690 695 700 Leu Leu Asn Gly Met Lys Thr Val Gly Giu Leu Phe Gly Ser Gly Gin 705 710 715 720 Met Gin Leu Pro Phe Val Leu Gin Ser Ala Giu Thr Met Lys Thr Ala 725 730 735 Vai Ala Tyr Leu Giu Pro Phe Met Giu Giu Giu Ala Giu Ala Thr Gly 740 745 750 Ser Ala Gin Ala Giu Giy Lys Gly Lys Ile Val Val Ala Thr Val Lys 755 760 765 Gly Asp Val His Asp Ile Gly Lys Asn Leu Val Asp Ile Ile Leu Ser 770 775 780 Asn Asn Gly Tyr Asp Val Val Asn Leu Giy Ile Lys Gin Pro Leu Ser 785 790 795 800 Ala Met Leu Giu Ala Ala Glu Giu His Lys Ala Asp Val Ile Gly Met 805 810 815 Ser Gly Leu Leu Val Lys Ser Thr Val Val Met Lys Gin Thr Ile Ser 820 825 830 Asp <210> 213 <211> 2578 <212> DNA <213> Corynebacteriun giutamicum <22 0> <221> CDS <222> (101)..(2578) <223> FRXA02198 <400> 213 agactagtgg cgctttgcct gtgttgctta ggcggcgttg aaaatgaact acgaatgaaa agttcgggaa ttgtctaatc cgtactaagc tgtctacaca atg tct act tca gtt 115 Met Ser Thr Ser Val 1 act tca cca gcc cac aac aac gca cat tcc tcc gaa ttt ttg gat gcg 163 Thr Ser Pro Ala His Asn Asn Ala His Ser Ser Giu Phe Leu Asp Ala 15 ttg gca aac cat gtg ttg atc ggc gac ggc gcc atg ggc acc cag ctc 211 307 WO 01/00843 WO 0100843PCTJIBOO/00923 Leu caa Gin tgt Cys cac Hius ttt Phe tgc Cys ga t Asp tcc Ser gc a Al a 150 gg t Gly gtc Val1 aca Thr atg Met c tg Leu 230 atg Met tcg Ala ggC Gly aat Asn cgc Arg gg t Gly cgt Arg gag Glu ctg Leu 135 gat Asp ggt Gly aag Lys ttc Phe c tc Leu 215 gg t Gly agc Ser g tg Asn His ttt gac Phe Asp gag att Glu Ile gcc tac Ala Tyr tgc aac Cys Asn gag ctt Glu Leu 105 atg ggg Met Gly 120 gga cct Gly Pro ttg cgt Leu Arg ggc gat Gly Asp gct gcg Ala Ala 185 ttg ccc Leu Pro 200 atg ggt Met Gly atc gac Ile Asp gag cac Giu His atg cct Val Leu Ile Gly Asp Gly Ala Met Gly ctt Leu gtg Val1 gtt Val1 gac Asp gc t Al a cg t Arg ggc Giy 145 ctt Leu cag Gin atg Met gag Glu aca Thr 225 acc Thr gcc Ala ggt Gly ga t Asp t tg Leu gag Giu a tc Ile agg Arg ttc Phe 130 cat His ggc Gly gac Asp gc t Al a ac c Thr 210 gcg Ala 9gc Giy gat Asp aaa Lys Thr Gln ctg gag Leu Glu agg cag Arg Gln acc aat Thr Asn gct gat Ala Asp 100 gaa gtg Glu Val 115 gtg gtt Val Val gca ccg Ala Pro atc atc Ile Ile ttg ctt Leu Leu 180 gaa ctt Giu Leu 195 acc ggc Thr Gly ctg cag Leu Gin cca gat Pro Asp att cct Ile Pro 260 aac ggt Asn Gly Leu ggg Giy att Ile act Thr cgt Arg gct Ala ggt Gly tat Tyr gac Asp 165 cag Gin gat Asp ac c Thr cca Pro gag Glu 245 gtg Val1 gca Ala Ser Val Met Pro Asn Ala Gly Leu Pro Val Leu WO 01/00843 WO 0100843PCT/iBOO/00923 265 Ct t Leu 270 t tg Leu gaa tac cca Glu Tyr Pro 280 gag gct gag Glu Ala Giu gat Asp 285 atg Met gcg cag gcg Ala Gin Ala gtc tcc 1027 Val Ser 295 cct gag 1075 Pro Glu 310 cag gaa 1123 Gin Glu gcc tcc 1171 Ala Ser tcg gtg 1219 Ser Val gaa tat ggc ctg Glu Tyr Gly Leu cac atc cgt gcg His Ile Arg Ala tcc Ser 300 gtg ggt ggt tgt Val Gly Gly Cys 305 275 ctg gct gga ttc Leu Ala Gly Phe 290 tgt ggc acc aca Cys Gly Thr Thr ggt gtt cca gag Gly Val Pro Giu 325 acc tcc aca Thr Ser Thr 330 cgc gag gtg Arg Giu Val gtc cgc gat Val Arg Asp acc aag atc Thr Lys Ilie aaa gag gac Lys Giu Asp gcg gtg Ala Val 320 gag gtt Val cca Pro 360 345 ttg Leu tcc cag gaa acc ggc Ser Gin Giu Thr Gly 365 350 cct gca ggc cct gtt gag cag Pro Ala Gly Pro Val Giu Gin 335 340 tcc gtc gcg tcg ctg tac acc Ser Val Ala Ser Leu Tyr Thr 355 att tcc atg atc ggt gag cgc Ile Ser Met Ile Gly Glu Arg 370 cgt gag gca atg ctg tct ggc Arg Giu Aia Met Leu Ser Gly 385 aag cag caa acc cgc gat ggt Lys Gin Gin Thr Arg Asp Gly acc aac 1267 Thr Asn 375 gat tgg 1315 Asp Trp 390 gca cac 1363 Ala His tcc aac ggt tcc aag Ser Asn Gly Ser Lys 380 gaa aag tgt gtg gat Glu Lys Cys Val Asp gca ttc Ala Phe att gcc Ile Ala gcc gat 1411 Ala Asp cca atc 1459 Pro Ile gag cac 1507 Glu His 455 atg ctg gat Met Leu Asp 410 atg gcg acc Met Ala Thr tgt gtg gat tac Cys Val Asp Tyr 415 gtg gga cga gac ggc acc Val Gly Arg Asp Gly Thr 420 400 ttg gca gca Leu Ala Ala 405 a tg Met 440 t tg Leu 425 att Ile ggt Gly ctt ctt gct acc agc tcc act ttg Leu Leu Ala Thr Ser Ser Thr Leu 430 435 cca gag gtt att cgc aca ggc ctt Pro Giu Val Ile Arg Thr Gly Leu gac tcc acc gag Asp Ser Thr Glu 445 gga cga agc atc Gly Arg Ser Ile gtt aac tcc gtc Val Asn Ser Val 465 450 aac ttt gaa gac Asn Phe Giu Asp 460 WO 01/00843 WO 0100843PCTIBOOIOO923 ggc gat 1555 Gly Asp 470 cag cac 1603 Gin Hius gca cgt 1651 Ala Arg gat atc 1699 Asp Ile tgc ctg 1747 Cys Leu 535 ggc at 1795 Gly Ile 550 gaa atc 1843 Giu Ilie cct gct 1891 Pro Ala gag gct 1939 Giu Ala atg aac 1987 Met Asn 615 tat gat 2035 Tyr Asp 630 ctg ttt 2083 Leu Phe ggc cct gag Gly Pro Glu ggt gcg gcc Gly Ala Ala 490 acc gct gag Thr Ala Giu icc cgc tac cag cgc atc Ser Arg Tyr Gin Arg Ile 475 480 gtg gtt gcg ctg acc att Vai Val Ala Leu Thr Ile 495 aig aaa ctg gta aag Met Lys Leu Val Lys 485 gat gag gaa ggc cag Asp Glu Giu Gly Gin 500 cac aag gig cgc Hius Lys Val Arg 505 acc ggc Thr Gly 520 agc tac ggc ctg Ser Tyr Gly Leu 525 att gct aaa cga ctg att gac Ile Ala Lys Arg Leu Ile Asp 515 atc aaa gac atc gtt gtg gac Ile Lys ASP Ile Val Val Asp 530 cag gaa gaa acc agg cga gat Gin Giu Giu Thr Arg Arg Asp acc ttc ccg atc Thr Phe Pro Ile gaa acc aic gaa Giu Thr Ile Giu ic t Ser 540 act Thr ggc Gly 545 cac acc acc His Thr Thr 570 gca cgc cag Ala Arg Gin gcc atc cgc Ala Ile Arg ggt ctg tcc Gly Leu Ser ctt aac tct Leu Asn Ser gag ctg Giu Leu 560 aag Lys aag cic tac cca Lys Leu Tyr Pro 565 gtt Val 585 ggt ctg Gly Leu 590 aat ait icc ttc ggc cig aac Asn Ile Ser Phe Gly Leu Asn 575 580 gtg ttc ctc aat gag tgc att Val Phe Leu Asn Giu Cys Ile 595 cac agc tcc aag ait ttg ccg His Ser Ser Lys Ile Leu Pro 610 gaa gig gcg itg gat atg gtc Giu Val Ala Leu Asp Met Val gac ict gcg ati Asp Ser Ala Ile 605 600 cgc Arg gcg Ala cgc Arg ati gat gat cgc Ile Asp Asp Arg 620 cag Gin cgc cgc acc gag Arg Arg Thr Giu 635 gag ggc gtt tct Giu Gly Val Ser 650 gat tac gat ccg Asp Tyr Asp Pro gct gcc gat gcc Ala Ala Asp Ala 655 625 ctg cag Leu Gin 640 aag gat Lys Asp gaa itc atg cag Glu Phe Met Gin 645 gct cgc gci gaa Ala Arg Ala Giu 660 WO 01/00843 WO 0100843PCT/IBOOIOO923 cag ctg 2131 Gin Leu gac ggc 2179 Asp Gly gag aag 2227 Giu Lys 695 aag acc 2275 Lys Thr 710 gtg ctg 2323 Val Leu ccg ttc 2371 Pro Phe ggc aag 2419 Gly Lys atc ggc 2467 Ile Gly 775 gtg gtg 2515 Val Val 790 gcg gaa 2563 Ala Giu aag tcc 2578 Lys Ser gcC gct Ala Ala 665 gat aag Asp Lys atg cct ttg ttt gag Met Pro Leu Phe Giu 670 aat ggc ctt gag gat Asn Gly Leu Giu Asp 685 att gcg atc atc aac Ile Ala Ile Ile Asn cgt ttg gca cag cgc atc atc Arg Leu Ala Gin Arg Ile Ile 675 gat ctg gaa gca ggc atg aag Asp Leu Glu Aia Gly Met Lys 690 gag gac ctt ctc aac ggc atg Giu Asp Leu LeU Asn Gly Met cc t Pro 700 gtg ggt gag ctg Val Gly Glu Leu 715 caa tcg gca gaa Gin Ser Ala Giu 730 atg gaa gag gaa Met Glu Giu Giu t tt Phe acc Thr 705 ggt tcc gga cag atg Gly Ser Gly Gin met 720 atg aaa act gcg gtg Met Lys Thr Ala Val cag ctg cca ttc Gin Leu Pro Phe 725 gcc tat ttg gaa Ala Tyr Leu Giu 735 .740 gca gaa Ala Giu gct acc gga tct gcg cag gca gag Ala Thr Gly Ser Ala Gin Ala Giu 750 755 745 ggc aaa Gly Lys atc gtc gtg gcc Ile Val Val Ala 765 acc gtc aag Thr Val LYS aac ttg gtg Asn Leu Val aac ttg ggc atc Asn Leu Gly Ile 795 gaa cac aaa gca Glu His Lys Ala 810 acc gtg gtg Thr Val Val 825 gac atc att ttg tcc Asp Ile Ile Leu Ser 780 aag cag cca ctg tcc Lys Gin Pro Leu Ser 800 gac gtc atc ggc atg Asp Val Ile Giy Met 815 ggt gac gtg cac gat Gly Asp Val His Asp 770 aac aac ggt tac gac Asn Asn Gly Tyr Asp 785 gcc atg ttg gaa gca Aia Met Leu Giu Ala 805 tcg gga ctt ctt gtg Ser Gly Leu Leu Val 820 <210> 214 <211> 826 <212> PRT <213> Cory-nebacteriun giutamicum WO 01/00843 PCTIIIBOO/00923 <400> 214 Met Ser Thr Ser Val Thr Ser Pro Ala H-is Asn Asn Ala His Ser Ser 1 5 Glu Met Leu Val Val

ASP

Ala Arg Gly 145 Leu Gin Met Giu Thr 225 Thr Ala Gly Ala Cys 305 Phe Gly Asp Leu Giu Ile Arg Phe 130 His Gly Asp Ala Thr 210 Ala Gly Asp Lys Leu 290 Cys Leu Thr Leu Arg Thr Ala Glu 115 Val1 Ala Ile Leu Giu 195 Thr Leu Pro Ile Asn 275 Ala Gly Asp Gin Giu Gin Asn Asp 100 Val Val Pro Ile Leu 180 Leu Giy Gin Asp Pro 260 Gly Gly Thr Ala Leu Gly Ile Thr Arg Ala Gly Tyr Asp 165 Gin Asp Thr Pro Giu 245 Val Ala Phe Thr Leu Gin Cys His Phe Cys Asp Ser Ala 150 Gly Val1 Thr Met Leu 230 Met Ser Giu Val1 Pro 310 Ala Gly Asn 55 Arg Gly Arg Giu Leu 135 Asp Gly Lys Phe Leu 215 Giy Ser Val1 Tyr Ser 295 Glu Ile Val Asp Ala Asn Lys Arg Lys 140 Tyr Leu Giy Cys Ile 220 Gly Tyr Gly Glu Ser 300 Val1 Gly Giu Thr Gly Leu Gly Asn 125 Leu Lys Ile Val1 Hius 205 Gly Leu Leu Leu Asp 285 Met Arg Asp Lys Arg Ala Ala Thr 110 Gly Pro Giu Giu Gin 190 Val Ala Asn Ser Pro 270 Leu Val Asp WO 01/00843 Val Gly Va Gly Pro Va Ala Ser Le Met Ilie Gi 370 Ala Met Le 385 Gin Thr Ar Gly Arg AS Thr Ser Se 43 Ile Arg Th 450 Val Asn Ph 465 Met Lys LE Asp Glu GI Lys Arg LE 51 Asp Ile Va 530 Giu Thr Ar 545 Lys Lys LE Ser Phe GI Leu Asn GI 59 Ser Lys Ii 610 Ala Leu As 625 Gin Giu Ph Gin Ala Ser Thr Asp 390 Ala Ala Pro Giu Giy 470 Gin Aia Asp Cys Giy 550 Giu Pro Glu Met Tyr 630 Leu Giu Ser Val Asn 375 Trp His Asp Ile His 455 Asp His Arg Ile Leu 535 Ile Ile Ala Ala Asn 615 Asp Phe Thr Arg Pro 360 Ser Giu Met Met Met 440 Leu Gly Gly Thr Thr 520 Thr Giu His Ala Gly 600 Arg Arg Glu Thr 330 Val1 Ser Gly Cys Asp 410 Thr Asp Giy Giu Ala 490 Giu Ser Pro Ile Thr 570 Gin Asp Asp Thr Val Thr Lys Giu Lys 380 Asp Cys Ala Thr Ser 460 Arg Val Lys Gly Ser 540 Ala Gly Leu Ala Arg 620 Asp Ala PCT/IBOO/00923 Pro Ala 335 Ser Val Ile Ser Arg Giu Lys Gin 400 Tyr Val 415 Leu Ala Giu Val Asn Ser Arg Ile 480 Thr Ile 495 Ile Ala Ile Lys Gin Giu Giu Leu 560 Asn Ile 575 Val Phe His Ser Giu Val Pro Leu 640 Ala Lys WO 01/00843 Asp Ala Glu Leu 705 Met Val Ser Gly Asn 785 Ala Ser Gin Asp Glu Lys 710 Val1 Pro Gly Ile Val 790 Ala Lys Leu Gly Lys 695 Thr Leu Phe Lys Gly 775 Val Glu Ser Leu Leu Ile 700 Phe Thr Ala Val1 Asp 780 Lys Asp PCT/IBOO/00923 655 Arg Leu Asp Leu Glu Asp Gly Gin 720 Thr Ala 735 Thr Gly Val Lys Leu Ser Leu Ser 800 Gly Met 815 <210> 215 <211> 621 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(598) <223'> RXN03074 <400> 215 tttgtgggca atctggtttt ttcgtaattg tgtgggatga atctcttaaa aattcacatt tagcaggaca agcatactgt tttagttcta tgctgtgggc atg act caa agt gct 115 Met Thr Gin Ser Ala 1 cca gaa ttc att gcc acc gca gac ctc gta gac atc atc ggc gac aac 163 Pro Glu Phe Ile Ala Thr Ala Asp Leu Val Asp Ile Ile Gly Asp Asn 15 gcg caa tca tgc gac act cag ttt caa aac ctt gga ggt gcc aca gaa 211 Ala Gin Ser Cys Asp Thr Gin Phe Gin Asn Leu Gly Gly Ala Thr Glu 30 WO 01/00843 ttC Phe c tg Leu gat Asp gga Gly att Ile ctt Leu gac Asp gtc c ac His aaa Lys ggc Gly ctt Leu cga Arg gga Gly gta Val1 135 tac gga Gly tcc Ser gac Asp gga Gly gac Asp ac c Thr 120 g tg Val1 gcg ata ata acc acc gtg aaa tgc ttc caa gac aac Ile Ile Thr Thr Val Lys Cys Phe Gin Asp Asn 45 agc Ser g tg Val 75 cat His gtc Val1 cgg Arg att Ile gac gag Giu cac His ggt Gly a tc Ile aaa Lys ggt Giy 140 gga Gly gat aat Asp Asn acc gcg Thr Ala tgg tcc Trp Ser ggc acc Gly Thr 110 tcc act Ser Thr 125 ggc att Giy Ile att atc Ile Ile cc t Pro c ta Leu gga Giy a tg Met aaa Lys gac Asp gtc Val ggg Gly gtt Val 80 gta Vali acc Thr act Thr t tc Phe acc Thr ctg Leu atc Ile aac Asn tgt Cys 115 ggC Giy gg t Giy cca Pro PCTIIBOOIOO923 gcc ctc 259 Ala Leu gtt atc 307 Val Ile att gca 355 Ile Ala gga gca 403 Giy Ala 100 aaa gcc 451 Lys Ala gaa cga 499 Giu Arg cat tac 547 His Tyr att aag 595 Ile Lys Vai Tyr Ala Asp Ser Asp cag Gin taatttgttt tgacgacgca gta <210> 216 <211> 166 <212> PRT <213> Corynebacterium giutamicum <400> 2i6 Met Thr Gin Ser Ala Pro Giu PheI 1 Ile Ile Gly Asp Asn Ala Gin SerC Gly Gly Ala Thr Giu Phe His Gly 40 Gin Asp Asn Ala Leu Leu Lys Ser Gly Val Leu Val Ile Asp Giy Asp 2 Gly Asp Ile Ile Ala Giy Leu Gly 1 Asp Phe Val1 Asp Thr Trp Leu Gin Lys Asn Ala Ser WO 01/00843 Ile Val Asn Phe Gly Cys 115 Gly Ser Gly 130 Ile Pro Gly 145 GiU Ala Pro PCTIBOO/00923 Ala Ile Arg Asp Ser Ala Val Ile Gly Thr Met Thr 105 110 Ala Leu Gly Thr Asn Pro Arg Lys Ser Thr Lys Thr 120 125 Arg Asp Val Val Val Ser Ile Gly Gly Ile Asp Phe 135 140 Tyr Val Tyr Ala Asp Ser Asp Gly Ile Ile Val Thr 150 155 160 Lys Gin 165 <210> 217 <21i> 621 <212> DNA <2i3> Corynebacterium giutamicum <220> <221> CDS <222> (101). .(598) <223> FRxAO2906 <400> 217 tttgtgggca atctggtttt ttcgtaattg tgtgggatga atctcttaaa aattcacatt tagcaggaca agcatactgt tttagttcta tgctgtgggc atg act caa agt gct Met Thr Gin Ser Ala cc a Pro gcg Ala t tc Phe ctg Leu ga t Asp gga Gly att Ile gcc Ala gac Asp ata Ile ctg Leu tcc Ser ga t Asp gca Ala acc Thr act Thr acc Thr agc Ser gtg Val cat His gtc Val1 gca Ala cag Gin acc Thr gag Giu cac His ggt Gly atc Ile gac Asp ttt Phe gtg Val1 ga t Asp ac c Thr tgg Trp ggc Gly c tc Leu caa Gin aaa Lys aat Asn gcg Ala t cc Ser acc Thr 110 ggc Gly gcc Ala aac Asn c tg Leu atc Ile aac Asn gac Asp ac a Thr gcc Ala gtt Val1 att Ile gga G ly 100 115 163 211 259 307 355 403 451 acc ttt ggt tgt aaa gcc Met Thr Phe Gly Cys Lys Ala 115 WO 01/00843 PCTIIBOO/00923 ctt gga acc aac ccg cgg aaa tcc act aaa act ggt tcc ggc gaa cga 499 Leu Gly Thr Asn Pro Arg Lys Ser Thr Lys Thr Gly Ser Gly Giu Arg 120 125 130 gac gta gtg gta tcg att ggt ggc att gac ttc att cct ggt cat tac 547 Asp Val Val Val Ser Ile Gly Giy Ile Asp Phe Ilie Pro Gly His Tyr 135 140 145 gtc tac gcg gac tct gac gga att atc gtc acc gag gcg cca att aag 595 Val Tyr Ala Asp Ser Asp Gly Ile Ile Val Thr Glu Ala Pro Ile Lys 150 155 160 165 cag taatttgttt tgacgacgca gta 621 Gin <210> 218 <211> 166 <212> PRT <213> Corynebacteriui giutamicum <400> 218 Met Thr Gin Ser Ala Pro Glu Phe Ilie 1 Ile Giy Gin Giy Gly Ilie Phe Giy Ilie 145 Giu Ser Gly Ser Asp Gly Asp Thr 120 Val1 Ala Cys Ile Ile Ala Lys Ser 105 Asn Val1 Asp Ala 10 Asp Ile Leu Ser Asp Ala Pro Ser Ser Thr Thr Thr Ser Vali Hius Val1 Arg Ile Asp 155 Val1 Asn Cys Pro Leu Gly Met Lys Asp Val <210> 219 <211> 1557 <212> DNA <213> Corynebacteriul glutamicum WO 01/00843 WO 0100843PCTJIBOO/00923 <220> <221> CDS <222> (101)..(1534) <223> RXN00132 <400> 219 aacagcttca atcaattcgg tgtccactcc aacatgtaga gtggtgcgcg ttaaaaaagt tttcctaatt ttcattttct taaaaggagc tcgccaggac atg gca cag gtt atg Met Ala Gin Val Met gac Asp att Ilie gaa Giu atc Ile t tg Leu ga t Asp cca Pro tac Tyr aac Asn ggt Gly 150 gat Asp gc t t tc Phe cgt Arg t tc Phe cac His ggC Giy gag Glu gct Ala tgg Trp, atg Met 135 cgc Arg tcc Ser gca aag Lys ctt Leu gc a Al a atg Met gc t Aia gc t Al a ggt Giy tgg Trp 120 atc Ile gaa Giu ga t Asp gag gt t Val gca Ala gac Asp acg Thr gag Glu gca Ala gtt Val1 105 tgc Cys ctc Leu tac Tyr gag Giu cc t gcc Ala gag Giu gag Glu gtc Val1 gtt Val1 gcg Ala cca Pro atc Ile gac Asp gag Glu tac Tyr 170 ggc Gly gat ctt tca cta Asp Leu Ser Leu tat gag atg cca Tyr Giu Met Pro 30 cag cct ttg aag Gin Pro Leu Lys 45 cag acc gcc gtg Gin Thr Ala Val cgt tgg gct tcc Arg Trp Ala Ser gct atc gtt gtc Ala Ile Val Val gta ttc gcg tgg Val Phe Ala Trp 110 aac cag atc ttc Asn Gin Ile Phe 125 gac ggc ggt gac Asp Giy Giy Asp 140 cag gct ggt ctg Gin Ala Gly Leu 155 atc gca ttc ttg le Ala Phe Leu aag tgg ggc aag Lys Trp Gly Lys gga Gly cag Gin att Ile ac c Thr ttc Phe acc Thr tca Ser gat Asp 130 gc t Ala gc a Ala cgt Arg gcc Ala cgt Arg ttg Leu gc t Ala ctc Leu tcc Ser gtc Val1 c tg Leu 115 gag Giu gtt Val gag Giu gag Glu gtt Val 195 Ala Ala Giu Pro 185 WO 01/00843 gtc acc gag Val Thr Glu 200 gaa gaa ggc Giu Giu Gly 215 acc aag tcc Thr Lys Ser 230 gac ggc atc Asp Gly Ile ctt gtc tgc Leu Val Cys gac ggc cag Asp Gly Gin 280 gct ctt cag 1027 Ala Leu Gin 295 gcc atc gag 1075 Ala Ile Giu 310 atc att tcc 1123 Ile Ile Ser ggc aac atc 1171 Giy Asn Ile cac cgc gac 1219 His Arg Asp 360 ttc acc ttc 1267 Phe Thr Phe 375 ctg ttg aac 1315 Leu Leu Asn 390 aac tct ttc 1363 acc Thr cc t Pro gat Asp 235 gcc Ala ggc Gly cgc Arg atg Met gac Asp 315 cag Gin ttt Phe acc Thr ggt Gly aac Asn 395 cag acc Thr ttc Phe 220 aac Asn act Thr gat Asp gtc Val1 ga t Asp 300 atc Ile atg Met gat Asp cgc Arg cgc Arg 380 gcc Ala acc ggt Gly 205 cca Pro aag Lys gac Asp g tc Val aag Lys 285 ggc Giy gtg Val c tc Leu aat Asn acc Thr 365 tcc Ser ac c Thr att cgc Arg aac Asn acc Thr 240 atg Met ggc Gly gaa Giu gtg Val1 gcg Ala 320 aag Lys gat Asp aag Lys gtc Val1 cca Pro 400 atc c tg Leu gtc Val1 225 cgc Arg ggc Gly tgc Cys gct Ala gtc Val 305 acc Thr gat Asp a tg Met cca Pro ctg Leu 385 tca Ser gaa c ac His gac Asp tcC Ser aag Lys gag Giu 275 cca Pro gtt Val1 aac Asn gc t Ala tcc S er 355 gtc Val1 gaa Giu gtc Val1 ttc PCTIBOO/00923 ttc gct 739 Phe Ala gct gtc 787 Ala Val ctg atc 835 Leu Ile 245 aac gtg 883 Asn Val 260 gct ttc 931 Ala Phe atc aac 979 Ile Asn gat gag Asp Giu aag gac Lys Asp 325 ctg ctg Leu Leu 340 ctg ttg Leu Leu gac gag Asp Giu ggt cgc Gly Arg atg tcc Met Ser 405 caa aac WO 01/00843 Asn Ser Phe Ala Asp Gin Thr Ile Ala 410 gaa gga cag tac gag aac gag gtc tac 1411 Giu Gly Gin Tyr Giu Asn Glu Val Tyr 425 430 gaa aag gtg gca cgc atc cac gtt gag 1459 Giu Lys Val Ala Arg Ilie His Val Glu 440 445 gaa ctg acc aag gag cag gct gag tac 1507 Glu Leu Th r Lys Glu Gin Ala Giu Tyr 455 460 cca ttc aag ccg gag cac tac cgc tac 1554 Pro Phe Lys Pro Glu His Tyr Arg Tyr 470 475 gga 1557 <210> 220 <211> 478 <212> PRT <213> Corynebacterium glutamicum <400> 220 Met Ala Gin Val Met Asp Phe Lys Val PCTIiBOO/00923 Gin Ile Giu Leu Phe Gin Asn 415 420 cgt ctg cct aag gtt ctc gac Arg Leu Pro Lys Val Leu Asp 435 gct ctc ggc ggt cag ctc acc Ala Leu Gly Gly Gin Leu Thr 450 atc ggc gtt gac gtt gca ggc Ile Gly Val Asp Val Ala Giy 465 taatgattgt cagcattgag 1 Ala Met Arg Giu Ilie Gly Giu Gly Gly Gin Ile Thr Phe Thr Ser Asp 130 Ile Giu Ile Leu 70

ASP

Pro Tyr Asn Arg Phe His 55 Gly Giu Ala Trp, Met 135 Ala Asp Thr Glu Ala Val1 105 Cys Leu Ala 10 Giu Glu Val Val1 Ala Pro Ile Asp Glu Asp Tyr Gin Gin Arg 75 Ala Val Asn Asp Gin Leu Giu Pro Thr Trp Ile Phe Gin Gly 140 Ala Ala Gly Gly Leu Cys Gly Lys Ser Ala Val1 Glu Leu Ala Ile Asn Ser Gly Trp Thr Pro Met Ala Val Ile Arg Gly Arg Giu Tyr WO 01/00843 145 Pro Ala Glu Leu Arg Glu Glu Ala Val 195 Leu Ty -r His 210 Val Asn Asp 225.

Arg His Ser Gly Gly Lys Cys Ala Glu 275 Ala Asp Pro 290 Val Thr Val 305 Thr Gly Asn Asp His Ala Met His Ser 355 Pro Gin Val 370 Leu Ser Glu 385 Ser Phe Val Giu Leu Phe Pro Lys Val 435 Gly Giy Gin 450 Val Asp Vai 465 Ala Asn Asp Ser Asp Giu Tyr Ile Ala Phe Leu G Val1 180 Lys Phe Ala Leu Asn 260 Ala Ile

ASP

Lys Leu 340 Leu Asp Gly Met Gin 420 Leu Leu Ala 165 Leu Gly Ala Val1 Ile 245 Val1 Phe Asn Giu Asp 325 Leu Leu Glu Arg Ser 405 Asn Asp Thr Giy Ala Val1 Glu Thr 230 Asp Leu Asp Al a Ala 310 Ile Gly His Phe Leu 390 Asn Giu Giu Glu Pro 470 170 Gly Thr Leu Phe Arg 250 Tyr Ala Leu Ala Giu 330 His Val1 Thr Gly Asp 410 Glu Arg Giu Giu Lys Thr Pro Asp 235 Ala Gly Arg Met Asp 315 Gin Phe Thr Giy Asn 395 Gin Asn Ile Gin His 475 Trp, Thr Phe 220 Asn Thr Asp Val1 Asp 300 Ile Met Asp Arg Arg 380 Ala Thr Giu His Ala 460 Tyr PCTIIBOO/00923 160 ly Met le Ala is Arg at Asn Ily Thr 240 au Met es Gly ftr Giu ar Val rir Ala 320 at Lys Le Asp l~e Lys L~e Val is Pro 400 Ln Ile r-g Leu La Leu L~e Gly WO 01/00843 WO 0100843PCT/IBOO/00923 <210> 221 <211> 128 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> <223> FR.XA00132 <400> 221 cac gtt gag gct ctc ggc ggt cag ctc acc His Val Glu Ala Leu Gly Gly Gin Leu Thr 1 5 10 gaa ctg acc aag Glu Leu Thr Lys gag cag Glu Gin gct gag tac atc ggc gtt gac gtt gca Ala Glu Tyr Ile Gly Val Asp Val Ala 25 ggc cca ttc aag Gly Pro Phe Lys ccg gag cac Pro Glu His tac cgc tac taatgattgt cagcattgag gga Tyr Arg Tyr <210> 222 <211> <212> PRT <213>-Corynebacteriun glutamictum <400> 222 His Val Giu Ala Leu Gly Gly Gin Leu TI Giu Leu Thr Lys Glu Gin Ala Glu Tyr Ilie Gly Val Asp Val Ala 25 Giy Pro Phe Lys Pro Glu His Tyr Arg Tyr <210> 223 <211> 1396 <212> DNA <2i3> Corynebacterium giutamicun <220> <221> <222> <223>

CDS

(101) (1396) FRXA01371 <400> 223 aacagcttca atcaattcgg tgtccactcc aacatgtaga gtggtgcgcg ttaaaaaagt tttcctaatt ttcattttct taaaaggagc tcgccaggac atg gca cag gtt atg Met Ala Gin Val Met gac ttc aag gtt gcc gat ctt tca cta gca gag gca gga cgt cac cag 163 Asp Phe Lys Val Ala Asp Leu Ser Leu Ala Giu Ala Gly Arg His Gln WO 01/00843 WO 0100843PCT11BOO/00923 15 att cgt ctt gca gag tat gag Atg cca ggt ctc atg cag ttg cgc aag 211 lie Arg Leu Ala Glu Tyr Glu Met Pro Gly Leu Met Gin Leu Arg Lys 30 gaa ttc gca gac gag cag cct ttg aag ggc gcc cga att gct ggt tct 259 Giu Phe Ala Asp Glu Gin Pro Leu Lys Gly Ala Arg Ile Ala Gly Ser 45 atc cac atg acg gtc cag acc gcc gtg ctt att gag acc ctc act gct 307 Ilie His Met Thr Val Gin Thr Ala Val LeuIle Glu Thr Leu Thr Ala 60 ttg ggc gct gag gtt cgt tgg gct tcc tgc aac att ttc tcc acc cag 355 Leu Gly Ala Glu Val Arg Trp Ala Ser Cys Asn Ile Phe Ser Thr Gin 75 80 gat gag gct gca gcg gct atc gtt gtc ggc tcc ggc acc gtc gaa gag 403 Asp Giu Ala Ala Ala Ala Ile Val Val Gly Ser Gly Thr Val Giu Giu 95 100 cca gct ggt gtt cca gta ttc gcg tgg aag ggt gag tca ctg gag gag 451 Pro Ala Gly Val Pro Val Phe Ala Trp Lys Gly Giu Ser Leu Giu Glu 105 110 115 tac tgg tgg tgc atc aac cag atc ttc agc tgg ggc gat gag ctg cca 499 Tyr Trp Trp Cys Ile Asn Gin Ile Phe Ser Trp Gly Asp Giu Leu Pro 120 125 130 aac atg atc ctc gac gac ggc ggt gac gcc acc atg gct gtt att cgc 547 Asn Met Ilie Leu Asp Asp Gly Gly Asp Ala Thr Met Ala Val Ile Arg 135 140 145 ggt cgc gaa tac gag cag gct ggt ctg gtt cca cca gca gag gcc aac 595 Gly Arg Glu Tyr Giu Gin Ala Gly Leu Val Pro Pro Ala Giu Ala Asn 150 155 160 165 gat tcc gat gag tac atc gca ttc ttg ggc atg ctg cgt gag gtt ctt 643 Asp Ser Asp Glu Tyr Ile Ala Phe Leu Gly Met Leu Arg Glu Val Leu 170 175 180 gct gca gag cct ggc aag tgg ggc aag atc gct gag gcc gtt aag ggt 691 Ala Ala Giu Pro Gly Lys Trp Gly Lys Ilie Ala Glu Ala Val Lys Gly 185 190 195 gtc acc gag gaa acc acc acc ggt gtg cac cgc ctg tac cac ttc gct 739 Val Thr Glu Glu Thr Thr Thr Gly Val His Arg Leu Tyr His Phe Ala 200 205 210 gaa gaa ggc gtg ctg cct ttc cca gcg atg aac gtc aac gac gct gtc 787 Glu Giu Gly Val Leu Pro Phe Pro Ala Met Asn Val AsnAsp Ala Val 215 220 225 acc aag tcc aag ttt gat aac aag tac ggc acc cgc cac tcc ctg atc 835 Thr Lys Ser Lys Phe Asp Asn Lys Tyr Gly Thr Arg His Ser Leu Ile 230 235 240 245 gac ggc atc aac cgc gcc act gac atg ctc atg ggc ggc aag aac gtg 883 Asp Gly Ile Asn Arg Ala Thr Asp Met Leu Met Gly Gly Lys Asn Val 250 255 260 WO 01/00843 Ott gtC tgc Leu Val Cys gac ggc cag Asp Gly Gin 280 PCT/EBOO/00923 ggt Gly 265 ggc Gly tao ggc gat gto ggc Tyr Gly Asp Val Gly aag ggo tgc gct Lys Gly Cys Ala gag got ttc 931 Giu Ala Phe 275 coa atc aao 979 Pro Ile Asn got cgc gtC Ala Arg Val aco gaa got Thr Giu Ala gac Asp 290 got Ott 1027 Ala Leu 295 gcc ato 1075 Ala Ile 310 atc att 1123 Ile Ile ggc aao 1171 Gly Asn oao ogc 1219 Hius Arg ttc aoo 1267 Phe Thr 375 otg ttg 1315 Leu Leu 390 aac tot 1363 Asn Ser gaa gga 1396 Glu Gly oag got otg atg Gin Ala Leu Met gag gao gco gao Giu Asp Ala Asp 315 too ttc gag oag Ser Phe Giu Gin 330 ato ggt cao ttt Ilie Giv His Phe tao tot gtg gtc aco gtt gat gag Tyr Ser Val Val Thr Val Asp Giu 305 ato gtg ato aoo gog Ile Val Ile Thr Ala 320 atg oto aag atg aag Met Leu Lys Met Lys 335 aoo ggo aao aag gao Thr Gly Asn Lys Asp 325 gat oao got otg otg Asp His Ala Leu Leu 340 gat aat gag Asp~ Asn Giu 345 gao gao Asp Asp 350 gtc aoo ogo aoo aog Val Thr Arg Thr Thr 365 aoo ggt ogo too ato Thr Gly Arg Ser Ile ato gat atg oat too otg ttg Ile Asp Met His Ser Leu Leu 355 atc aag coa oag gto gao gag Ile Lys Pro Gin Vai Asp Giu 370 ato gto otg too gaa ggt ogo Ile Val Leu Ser Giu Giy Arg 360 t to Phe too Ser 380 aao ott ggo aao Asn Leu Gly Asn 395 tto goc gat oag Phe Ala Asp Gin 410 oag tac gag aao Gin Tyr Giu Asn 425 gc Ala aoo gga oao oca Thr Gly His Pro 400 toa ttt gto atg too Ser Phe Val Met Ser 405 gaa otg tto oaa aao Glu Leu Phe Gin Asn 420 aoo att gog oag ato Thr Ile Ala Gin Ile 415 gag gto tao ogt otg Giu Vai Tyr Arg Leu 430 <210> 224 <211> 432 <212> PRT <213> Corynebacterium giutamioum <400> 224 WO 01/00843 Met Ala G: 1 Ala Gly A] Met Gin L( Arg Ilie A: Giu Thr L( Ile Phe Si Gly Thr Vi Giu Ser L( 1., Gly Asp G: 130 Met Ala V 145 Pro Ala G' Leu Arg G: Giu Ala Vi 1! Leu Tyr H: 210 Val Asn Ai 225 Arg His S( Gly Gly L Cys Ala G' 2 Ala Asp Pi 290 Val Thr V 305 Asp Ile Giu Ile Leu 70 Asp Pro Tyr Asn Gly 150 Asp Ala Val1 Giu Thr 230 Asp Leu Asp Ala Ala 310 Phe Arg Phe His 55 Gly Glu Ala Trp Met 135 Arg Ser Ala Thr Glu 215 Lys Gly Val Gly Leu 295 Ile Val Ala Asp Leu 10 Ala Glu Tyr Giu Asp Giu Gin Pro Thr Val Gin Thr Glu Val Arg Trp 75 Ala Ala Ala Ilie Val Pro Val Phe 105 Cys Ile Asn Gin Leu Asp Asp Gly 140 T1yr Giu Gin Ala 155 Glu Tyr Ile Ala 170 Pro Giy Lys Trp 185 Giu Thr Thr Thr Vai Leu Pro Phe 220 Lys Phe Asp Asn 235 Asn Arg Aia Thr 250 Gly Tyr Giy Asp 265 Gly Ala Arg Val Ala Leu Met Asp 300 Asp Ala Asp Ile 315 Leu Pro Lys Val1 Ser Val1 Trp 110 Phe Asp Leu Leu Lys 190 Val1 Al a Tyr Met Gly 270 Val1 Tyr Ile PCT/1B00/00923 Ala Giu Gly Leu Gly Ala Leu Ile Cys Asn Gly Ser Lys Gly Ser Trp Ala Thr Val Pro 160 Giy Met 175 Ile Ala His Arg Met Asn Gly Thr 240 Leu Met 255 Lys Gly Thr Giu Ser Val Thr Ala 320 Thr Gly Asn Lys Asp Ile Ile Ser Phe Giu Gin Met Leu Lys Met Lys WO 01/00843 WO 0100843PCTIIBOOIOO923 Asp Met Pro Leu 385 Ser Giu Gly His Phe Leu 390 Asn Giu Asn Arg Thr 375 Leu Ser Gly 330 His Val Thr Giy Asp 410 Giu 335 Ile Ile Ile His Gin 415 Arg <210> 225 <211> 2358 <212> DNA <213> Corynebacterium glutamicum <2 <221> CDS <222> (101)..(2335) <223> RXN02085 <400> 225 cacccggtga tttcgcgaac cttgaaacat cgtcagaaga gatccgcacg ttcggctcaa gcagaaagtc tttaactcac tc t Ser aag Lys ctt Leu tc t Ser atg Met ggt Gly ggc Gly cgc Arg gt.t Val1 gct Ala 75 ctt Leu tac Tyr caa Gin ccg Pro att Ile ttgccgtgcg tcctagccgg atg act tcc aac ttt Met Thr Ser Asn Phe 1 gcg aag cgt gaa ctg Ala Lys Arg Giu Leu att gaa ggt cgc gaa Ile Glu Gly Arg Giu gca tcg gat tct ttg Ala Ser Asp Ser Leu tcc tac tac gac gca Ser Tyr Tyr Asp Ala ccg gag cgt ttt gat Pro Glu Arg Phe Asp WO 01/00843 gac atc gct Asp Ile Ala tac ttt ggc Tyr Phe Giy acc aag tgg Thr Lys Trp 120 gcg gat aca Ala Asp Thr 135 cgt tgc cag Arg Cys Gin 150 cca ctg act Pro Leu Thr ttg gat cac Leu Asp His tct ttc gat Ser Phe Asp 200 gat gtt gct Asp Val Ala 215 ttg gct aag Leu Ala Lys 230 gat cag gcg Asp Gin Ala gtt gac ttg Vai Asp Leu gag gag ctg Giu Giu Leu 280 acc gac ctg 1027 Thr Asp Leu 295 ggc cca atc 1075 Gly Pro Ile 310 gat Asp gc t Ala 105 ttt Phe cgt Arg cag Gin t tc Phe c tg Leu 185 act Thr cc t Pro cgc Arg c tg Leu gtc Vai 265 ctg Leu tgt Cys gca Ala cat His gc t Ala gat Asp ttc Phe gt t Vai ctt Leu 170 cc t Pro gag Glu gag Giu gat Asp aac Asn 250 acc Thr gtt Val gc t Ala gtg Vali gaa Giu cgc Arg ac c Thr gtt Val cgt Arg 155 tcc Ser gca Ala tgg Trp gtt Vai Gly 235 act Thr cat His gcg Ala gc t Ala tc t Ser 315 aac Asn ggt Gly aac Asn t tg Leu 140 ggC Gly ctt Leu ctg Leu gtt Vai t tg Leu 220 g tg Val ctt Leu ggc Giy ggc Gly ctt Leu 300 acc Thr ga t Asp act Thr tac Tyr 125 ga t Asp gtt Val1 gc t Ala ttt Phe cag Gin 205 gag Giu ttt Phe gcg Ala gtc Val atc Ile 285 gc t Ala tc t Ser ggt Giy gag Giu 110 cac His gcg Al a aat Asn cgc Arg gag Giu 190 atc Ile cag Gin gtc Val1 Gly act Thr 270 gtt Val tcc Ser tgt Cys c tc Leu 95 acc Thr t ac Tyr tcc Ser gcc Al a acc Thr 175 gtc Val1 gat Asp gtc Val aa t Asn atc Ile 255 gag Glu ga t Asp ctg Leu tca ctg Leu cc t Pro gtg Val c tg Leu 145 cct Pro gat

ASP

gag Glu cct Pro gc t Ala 225 tac Tyr ctt Leu gc t Ala cgt Arg cgc Arg 305 tgg Trp gca Ala ccg Pro 130 att Ile gtt Val1 gg t Giy cgc Arg gcg Ala 210 ggt Gly t tc Phe ggc Gly gcg Ala a ac Asn 290 c tg Leu att Ile cag Gin 115 gag Glu gag Glu c tg Leu tcc Ser ctc Leu 195 ttg Leu tac Tyr ggc Gly gcg Ala tgg Trp 275 att Ile gca Ala PCTIBOO/00923 gac cgc 403 Asp Arg 100 gca atg 451 Ala Met ttg tct 499 Leu Ser gat ctc 547 Asp Leu gtt ggt 595 Val Gly 165 aat cct 643 Asn Pro 180 atc aag 691 Ile Lys gtc acc 739 Val Thr acc act 787 Thr Thr tct ggc 835 Ser Gly 245 att ggc 883 Ile Gly 260 aag ggt 931 Lys Giy tgg cgc 979 Trp Arg gct cgc Ala Arg ctg cac gtt cct tac Ser Leu Leu His Val Pro Tyr WO 01/00843 WO 0100843PCTIBOO/00923 acc ctc 1123 Thr Leu ttc ggc 1171 Phe Giy gcc ggc 1219 Ala Gly gct tct 1267 Ala Ser 375 cct ggC 1315 Pro Gly 390 aag tca.

1363 Lys Ser cca cag 1411 Pro Gin tcc atc 1459 Ser Ile ctg gtc 1507 Leu Vai 455 ggt gag 1555 Gly Glu 470 gac ggt 1603 Asp Gly cgc tgt 1651 Arg Cys gag Glu tcg Ser aac Asn 360 cga Arg cg t Arg ctg Leu ac c Thr act Thr 440 atc Ile cca Pro t tc Phe gtt Val1 gct Ala gag Glu 345 atc Ile cgc Arg agc Ser gag Giu cca Pro 425 ttg Leu gcc Ala gag Glu ctc Leu cgt Arg 505 gag Giu 330 aag Lys gac Asp acc Thr cgt Arg ctt Leu 410 tcc Ser gag Giu aag Lys cgc Arg tca Ser 490 cct Pro att Ile acc Thr gc t Ala cca Pro 380 tcc Ser gc t Ala cgt Arg tac Tyr gaa Glu 460 gac Asp gcc Al a gtg Val1 gag Glu gag Giu gcg Ala 365 cgc Arg ttc Phe c tg Leu tct Ser gaa Giu 445 gaa Giu atg Met aac Asn t tg Leu CC t Pro gtc Val 350 t tc Phe acc Thr gac Asp cca Pro gc t Ala 430 gag Glu ctt Leu gtt Val1 ggc Gly ttc Phe 510 gag Giu 335 aag Lys ga t Asp gca Ala act Thr acc Thr 415 cgc Arg gca Ala ggt Gly cag Gin tgg Trp, 495 gga Gly gtc Val c tg Leu gcg Al a cca Pro cgt Arg 400 acc Thr gc t Ala atg Met ctt Leu tac Tyr 480 gtc Val aac Asn cgc Arg C tt Leu gcg Ala a tc Ile 385 gtt Val1 acc Thr cgt Arg cgc Arg ga t Asp 465 t tc Phe caa Gin gtt Val1 gac Asp gcc Ala tcc Ser 370 acg Thr acg Thr att Ile ctg Leu gaa Glu 450 g tg Val tct Ser agc Ser tcc Ser ctt Leu 340 gcc Ala gca Ala gaa Giu cag Gin tc t Ser 420 aag Lys atc Ile gtt Val1 ctt Leu ggc Gly 500 cca Pro gcc Ala cta Leu att Ile ctc Leu gag Glu 405 ttC Phe gaa Giu gat Asp cac His C tc Leu 485 tcc Ser gcg Ala WO 01/00843 WO 0100843PCTIiBOO/00923 cca atg 1699 Pro Met cat gtc 1747 His Val 535 ttc gtt 1795 Phe Val 550 ctg gca 1843 Leu Ala atc atc 1891 Ile Ile gac gtc 1939 Asp Val gtc aag tgg ttc cag Val Lys Trp Phe Gin 525 tac gca cag agc ctg acc cag aag Tyr Ala Gin Ser Leu Thr Gin Lys 530 cca gtc acc atc ctt gca tgg tcc Pro Val Thr Ile Leu Ala Trp, Ser 545 aag gga atg ctc Lys Gly Met Leu cgc gat gat cag Arg Asp Asp Gin 555 ctg cgc gat gaa Leu Arg Asp Giu 570 cag gtg gat gag Gin Val Asp Giu acc ggt Thr Gly ccg ctg gct Pro Leu Aia att aac gat Ile Asn Asp cct gcg att Pro Ala Ile 590 tac ctg cag Tyr Leu Gin acc act Thr Thr 560 gct Ala gac cag gtt gca Asp Gin Val Ala 565 585 gat aag Asp Lys 600 ctc atc gag gct ggc gcg aag Leu Ile Giu Ala Gly Ala Lys 575 580 cgt gaa ctg ttg ccg cta cga Arg Giu Leu Leu Pro Leu Arg 595 tgg tcc gtg gac tcc ttc cgc Trp, Ser Val Asp Ser Phe Arg 610 gtc caa atc cac acc cac atg Val Gin Ile His Thr His Met cct gcc Pro Ala 605 ctg gcg act 1987 Leu Ala Thr 615 tgc tac tcc 2035 Cys Tyr Ser 630 gcc gat gtc 2083 Ala Asp Val gcc ggc gca Ala Gly Ala gag ttc aac Glu Phe Asn 635 acc acc atc Thr Thr Ile ccc Pro 620 gac Asp gac Asp 625 gaa gtg atc tc Giu Val Ile Ser gaa gca gca cgt Giu Ala Ala Arg 655 ggc ttc gag ctc Giy Phe Glu Leu tcg Ser 640 gtc Val atc gcg ttg gat Ile Ala Leu Asp 645 gct gct 2131 Ala Ala ctg aaa Leu Lys 665 tcc Ser tcc gac atg cag gtc ctc Ser Asp Met Gin Val Leu 660 ggc gtc gga. cct ggt gtg Gly Val Gly Pro Gly Val 675 gcg cag aaa gtg gac ggt Ala Gin Lys Val Asp Gly 670 tgg gat atc 2179 Trp Asp Ile 680 ctc ctc gag 2227 cac tcc ccg cgc gtt His Ser Pro Arg Val 685 gct gca ctg cag tcc cct tcc Pro Ser 690 gtg gat cct cgc cag ctg tgg gtc Leu Leu Giu Ala Ala Leu Gin Ser Val Asp Pro Arg Gin Leu Trp Val 695 700 705 WO 01/00843 aac cca gac 2275 Asn Pro Asp 710 tcc cta aag 2323 Ser Leu Lys PCTIBOO/00923 ggt ctg aag acc cgt gga tgg cca gaa gtg gaa gct Giy Leu Lys Thr Arg Giy Trp, Pro Giu Val Giu Ala 715 720 725 ctc gtt gag tcc gct aag cag gct cgt gag aaa atc Leu Val Giu Ser Ala Lys Gin Ala Arg Giu Lys Ile 730 735 740 taaattgggt taccgctagg aac gga gca 2358 Gly Ala act atc Thr Ile 745 <210> 226 <211> 745 <212> PRT <213> Corynebacterium giutamicum <400> 226 Met Thr Ser Asn Phe Ser Ser Thr Val 1 Ala Ile Ala Ser Pro Leu Pro Val1 Leu 145 Pro Asp Glu Lys Giu Ser Tyr Giu Trp Ala Pro 130 Ile Val1 Gly Arg Arg Gly Asp Tyr Arg Ile Gin 115 Giu Giu Leu Ser Leu 195 Lys Leu Ser Met 70 Asp Tyr Thr Ala Arg 150 Pro Leu Ser Phe Ala Gly 55 Leu Ile Phe Lys Asp 135 Cys Leu Asp Phe Ala Gin 40 Leu Asp Ala Gly Trp, 120 Thr Gin Thr His Asp 200 Leu Thr Asp Thr Asp Al a 105 Phe Arg Gin Phe Leu 185 Thr Ala 10 Glu Ala Ser Ala His Ala Asp Phe Val Leu 170 Pro Gi u Gly Gly Arg Val Ala 75 Giu Arg Thr Val1 Arg 155 Ser Ala Trp, Leu Tyr Gin Pro Ile Asn Gly Asn Leu 140 Gly Leu Leu Val1 Pro Trp Leu Phe Leu Asp Thr Tyr 125 Asp Val Ala Phe Gin 205 Arg Asn Val1 Ala Gly Gly Giu 110 His Ala Asn Arg Glu 190 Ile Ile Gly Asn Gly Val Leu Thr Tyr Ser Ala Thr 175 Val1 Asp Gly Ser Thr Arg Leu Pro Leu Leu Ala Arg 160 Thr Tyr Glu WO 01/00843 Pro Ala Leu 210 Ala Gly Tyr 225 Tyr Phe Giy Leu Gly Ala Ala Ala Trp 275 Arg Asn Ile 290 Arg Leu Ala 305 Leu His Val Arg Asp Trp Leu Ala Asp 355 Ala Ser Ala 370 Ile Thr Gin 385 Val Thr Leu Thr Ile Gly Arg Leu Arg 435 Arg Giu Giu 450 Asp Val Leu 465 Phe Ser Glu Gin Ser Tyr Vai Ser Arg 515 Ser Leu Thr Val1 Thr S er Ile 260 Lys Trp Ala Pro Leu 340 Ala Ala Glu Gin Ser 420 Lys Ile Val1 Leu Gly 500 Pro Gin Val1 Gly 235 Thr His Al a Ala Ser 315 Asn Ile Ala Ser Gly 395 Pro Ile Gin Gin Asn 475 Thr Pro Trp Leu Leu 220 Val1 Leu Gly Gly Leu 300 Thr Ile Thr Al a Pro 380 Ser Ala Arg Tyr Giu 460 Asp Ala Val1 Phe Thr PCTIBOO/00923 Val Arg Asn Thr 240 Ile Gly 255 Glu Leu Asp Gly Leu Lys Ser Leu 320 Giu Val 335 Lys Leu Asp Ala Ala Pro Thr Arg 400 Thr Thr 415 Arg Ala Ala Met Gly Leu Gin Tyr 480 Trp Val 495 Gly Asn Ala Gin Val Thr WO 01/00843 PCT/-BOO/00923 530. 535 540 Ile Leu Ala Trp, Ser Phe Val Arg Asp Asp Gin Pro Leu Ala Thr Thr 545 550 555 560 Ala Asp Gin Val Ala Leu Ala Leu Arg Asp Giu Ile Asn Asp Leu Ile 565 570 575 Giu Ala Giy Ala Lys Ile Ile Gin Val Asp Glu Pro Ala Ile Arg Giu 580 585 590 Leu Leu Pro Leu Arg Asp Val Asp Lys Pro Ala Tyr Leu Gin Trp, Ser 595 600 605 Val Asp Ser Phe Arg Leu Ala Thr Ala Gly Ala Pro Asp Asp Val Gin 610 615 620 Ilie His Thr His Met Cys Tyr Ser Giu Phe Asn Giu Vai Ile Ser Ser 625 630 635 640 Val Ilie Ala Leu Asp Ala Asp Val Thr Thr Ile Giu Ala Ala Arg Ser 645 650 655 Asp Met Gin Val Leu Ala Ala Leu Lys Ser Ser Giy Phe Giu Leu Gly 660 665 670 Val Gly Pro Gly Vai Trp, Asp Ile His Ser Pro Arg Val Pro Ser Ala 675 680 685 Gin Lys Val Asp Gly Leu Leu Glu Ala Ala Leu Gin Ser Val Asp Pro 690 695 700 Arg Gin Leu Trp Val Asn Pro Asp Cys Gly Leu Lys Thr Arg Gly Trp 705 710 715 720 Pro Giu Val Giu Ala Ser Leu Lys Val Leu Val Giu Ser Ala Lys Gin 725 730 735 Ala Arg Giu Lys Ilie Gly Ala Thr Ile 740 745 <210> 227 <211> 1923 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1900) <223> FRXA02085 <400> 227 cacccggtga tttcgcgaac cttgaaacat cgtcagaaga ttgccgtgcg tcctagccgg gatccgcacg ttcggctcaa gcagaaagtc tttaactcac atg act tcc aac ttt 115 Met Thr Ser Asn Phe 1 tct tcc act gtc gct ggt ctt cct cgc atc gga gcg aag cgt gaa ctg 163 Ser Ser Thr Val Ala Gly Leu Pro Arg Ilie Gly Ala Lys Arg Glu Leu 332 WO 01/00843 WO 0100843PCT/IEB00100923 aag ttc gcg Lys Phe Ala gaa ggc tac tgg Glu Giy Tyr Trp gga tca att gaa Gly Ser Ile Giu ggt cgc gaa Gly Arg Giu gat tct ttg Asp Ser Leu ctt gcg cag acc gcc cgc caa Leu Ala Gin Thr Ala Arg Gin tct gga ttg gat tcc gtt ccg Ser Gly Leu Asp Ser Val Pro t tg Leu gtc aac act gca Val Asn Thr Ala ttt gca gga cgt Phe Ala Gly Arg tac tac gac gca Tyr Tyr Asp Ala atg Met ctc gat acc gcc Leu Asp Thr Ala att ttg ggt gtg Ile Leu Gly Val ccg gag cgt ttt Pro Giu Arg Phe gac atc gct gat Asp Ile Ala Asp gaa aac gat ggt Giu Asn Asp Gly cca ctg tgg att Pro Leu Trp Ile gac cgc Asp Arg 100 tac ttt ggc Tyr Phe Gly acc aag tgg Thr Lys Trp 120 gct cgc ggt act Ala Arg Giy Thr acc ctg cct gca Thr Leu Pro Ala cag gca atg Gin Ala Met 115 gag ttg tct Giu Leu Ser ttt gat acc aac Phe Asp Thr Asn tac Tyr 125 cac tac ctc gtg His Tyr Leu Val gcg gat Ala Asp 135 aca cgt ttc gtt Thr Arg Phe Val gat gcg tcc gcg ASP Ala Ser Ala att gag gat ctc Ile Giu Asp Leu cgt Arg 150 tgc cag cag gtt Cys Gin Gin Val ggc gtt aat gcc Gly Val Asn Ala cct gtt ctg gtt Pro Val Leu Val ggt Gly 165 cca ctg act ttc Pro Leu Thr Phe tcc ctt gct cgc Ser Leu Ala Arg act gat ggt tcc Thr Asp Gly Ser aat cct Asn Pro 180 ttg gat cac Leu Asp His tct ttc gat Ser Phe Asp 200 cct gca ctg ttt Pro Ala Leu Phe gtc tac gag cgc Val Tyr Giu Arg ctc atc aag Leu Ile Lys 195 ttg gtc acc Leu Val Thr act gag tgg gtt Thr Giu Trp Val cag Gin 205 atc gat gag cct Ile Asp Giu Pro gat gtt Asp Val 215 gct cct gag gtt Ala Pro Giu Val gag cag gtc cgc Giu Gin Val Arg ggt tac acc act Gly Tyr Thr Thr ttg Leu 230 gct aag cgc gat Ala Lys Arg Asp gtg ttt gtc aat Val Phe Val Asn tac ttc ggc tct Tyr Phe Gly Ser ggC Gly 245 787 835 883 gat cag gcg ctg Asp Gin Ala Leu act ctt gcg ggc Thr Leu Ala Gly ggc ctt ggc gcg Gly Leu Gly Ala att ggc Ile Gly 260 WO 01/00843 gtt gac ttg Val Asp Leu gag gag ctg Glu Glu Leu 280 gtc Val1 265 ct Leu acc cat ggc gtc Thr His Gly Val PCTIBOOIOO923 aag ggt 931 Lys Giy gag ctt gct Glu Leu Ala gat ggt cgt Asp Gly Arg gcg Ala tgg Trp, 275 gtt gcg ggc Val Ala Giy aac att tgg cgc Asn Ile Trp, Arg 290 cig gca gct cgc Leu Ala Ala Arg 979 acc gac 1027 Thr Asp 295 ggc cca 1075 Gly Pro 310 acc ctc 1123 Thr Leu ttc ggc 1171 Phe Gly gcc ggc 1219 Ala Gly gci tct 1267 Ala Ser 375 cct ggc 1315 Pro Gly 390 aag tca 1363 Lys Ser cca cag 1411 Pro Gin tcc atc 1459 Ser Ilie ctg gtc 1507 ctg tgt gct gct Leu Cys Ala Ala atc gca gig ict Ilie Ala Val Ser 315 gag gct gag aac Giu Ala Giu Asn 330 icc ctg aag cgc Ser Leu Lys Arg 305 acc ici igi ica ctg Thr Ser Cys Ser Leu 320 ait gag cci gag gic Ile Giu Pro Glu Val ctg cac gtt cci tac Leu His Val Pro Tyr 325 cgc gac tgg cit gcc Arg Asp Trp Leu Ala 340 tcg gag Ser Glu 345 aac aic Asn Ile aag aic acc gag gic Lys Ile Thr Glu Val 350 gac gcg gct gcg tic Asp Ala Ala Ala Phe ctg cit gcc gac gcc cia Leu Leu Ala Asp Ala Leu 355 gat gcg gcg icc gca gca at Asp Ala Ala Ser Ala Ala Ile 370 gca cca atc acg cag gaa ctc Ala Pro Ile Thr Gin Giu Leu cga cgc acc icc Arg Arg Thr Ser cgi agc cgt gga Arg Ser Arg Gly 395 cig gag cit cca Leu Glu Leu Pro 410 acc cca tcc att Thr Pro Ser Ile acc Thr 385 tcc tic gac act cgt Ser Phe ASP Thr Arg 400 gct ctg cca acc acc Ala Leu Pro Thr Thr 415 cgt ict gct cgc gct Arg Ser Ala Arg Ala gtt acg ctg cag gag Val Thr Leu Gin Glu 405 acc att ggt tct tic Thr Ile Gly Ser Phe 420 act Thr 425 430 cgt ctg cgc aag gaa Arg Leu Arg Lys Glu 435 cgc gaa gaa aic gat Arg Giu Glu Ile Asp ttg gag cag tac gaa Leu Glu Gin Tyr Glu 445 gcc aag cag gaa gaa gag gca atg Giu Ala Met cit ggt ctt 450 gat gtg tig gtt cac WO 01/00843 Leu Val Ile 455 ggt gag cca 1555 Gly Glu Pro 470 gac ggt ttc 1603 Asp Gly Phe cgc tgt gtt 1651 Arg Cys Val cca atg act 1699 Pro Met Thr 520 cat gtc aag 1747 His Val Lys 535 ttc gtt cgc 1795 Phe Val Arg 550 ctg gca ctg 1843 Leu Ala Leu atc atc cag 1891 Gin aac Asfl 475 acc Thr cca Pro tgg Trp ctc Leu cag Gin 555 gaa Glu gag Glu 460 gac Asp gcc Ala gtg Val1 ttc Phe acc Thr 540 ccg Pro att Ile cc t Leu tac Tyr 480 gtc Val aac Asn cag Gin acc Thr act Thr 560 atc Ile gaa Giu Val tc t Ser agc Ser tcc Ser ctg Leu 530 ctt Leu gac Asp gc t Ala ttg Leu Leu gaa Giu tac Tyr cgc Arg 515 acc Thr gca Ala cag Gin Giy ccc Pro PCT/IIBOO/00923 Vai His ctt ctc Leu Leu 485 ggc tcc Gly Ser 500 cca gcg Pro Ala cag aag Gin Lys tgg tcc Trp Ser gtt gca Val Ala 565 gcg aag Ala Lys 580 gct acg Ala Thr Ile Ile Gin Val Asp Glu Pro Ala Ile Arg 585 590 aga cgt 1923 Arg Arg cga Arg 600 taagcctgcc tacctgcagt ggt <210> 228 <211> 600 <212> PRT <213> Corynebacterium giutamicui <400> 228 Met Thr Ser Asn Phe Ser Ser Thr Val Ala Gly Leu Pro Arg Ile Giy 1 5 10 Ala Lys Arg Glu Leu Lys Phe Ala Leu Giu Giy Tyr Trp Asn Gly Ser 25 WO 01/00843 Ile Giu Gly Ala Ser Asp Ser Tyr Tyr Pro Giu Arg Leu Trp Ile Pro Ala Gin 115 Val Pro Glu 130 Leu Ile Glu 145 Pro Val Leu Asp Gly Ser Glu Arg Leu 195 Pro Ala Leu 210 Ala Gly Tyr 225 Tyr Phe Gly Leu Gly Ala Ala Ala Trp 275 Arg Asn Ile 290 Arg Leu Ala 305 Leu His Val Arg Asp Trp Leu Ala Asp Arg Ser Asp Phe Asp 100 Ala Leu Asp Val1 Asn 180 Ile Val Thr Ser Ile 260 Lys Trp Ala Pro Leu 340 Ala Giu Leu Ala Asp Arg Met Ser Lau Gly 165 Pro Lys Thr Thr Gly 245 Gly Gly Arg Arg Tyr 325 Ala Lau Leu Ser Met 70 Asp Tyr Thr Ala Arg 150 Pro Leu Ser Asp Leu 230 Asp Val1 Glu Thr Giy 310 Thr Phe Ala Ala Gly 55 Leu Ile Phe Lys Asp 135 Cys Leu Asp Phe Val 215 Ala Gin Asp Glu Asp 295 Pro Leu Gly Gly Gin 40 Leu Asp Ala Gly Trp 120 Thr Gin Thr His Asp 200 Ala Lys Ala Leu Leu 280 Leu Ile Glu Ser Asn Arg Vali Aia 75 Giu Arg Thr Vali Arg 155 Ser Al a Trp Val Gly 235 Thr His Al a Ala Ser 315 Asn Ile Gin Pro Ile Asn Gly Asn Leu 140 Gly Leu Leu Val Leu 220 Val1 Leu Gly Gly Leu 300 Thr Ile Thr Leu Phe Leu Asp Thr Tyr 125 Asp Val1 Ala Phe Gin 205 Glu Phe Ala Val1 Ile 285 Ala Ser Glu Giu Val Ala Gly Gly Giu 110 His Aia Asn Arg Giu 190 Ile Gin Val Gly Thr 270 Val Ser Cys Pro Val 350 Phe PCT/1B00100923 Asn Thr Gly Arg Val Leu Leu Pro Thr Leu Tyr Leu Ser Aia Ala Arg 160 Thr Thr 175 Val Tyr Asp Glu Val Arg Asn Thr 240 Ile Gly 255 Glu Leu Asp Gly Leu Lys Ser Leu 320 Giu Val 335 Lys Leu Asp Ala Asp Ala Ala Ala WO 01/00843 WO 0100843PCT/IBOO/00923 Ala Ile 385 Vai Thr Arg Arg Asp 465 Phe Gin Val Ser Ile 545 Ala Giu Leu Ser 370 Thr Thr Ilie Leu Giu 450 Val Ser Ser Ser Leu 530 Leu Asp Ala Leu 355 Ala Gin Leu Gly Arg 435 Giu Leu Giu Tyr Arg 515 Thr Ala Gin Gly Pro 595 Ala Ile Ala Ser Arg Arg Thr Ser Pro Arg Thr Ala Pro Glu Gin Ser 420 Lys Ile Val1 Leu Gly 500 Pro Gin Trp Vali Ala 580 Ala Leu Giu 405 Phe Glu Asp His Leu 485 Ser Ala Lys Ser Ala 565 Lys Thr Pro 390 Lys Pro S er Leu G ly 470 Asp Arg Pro His Phe 550 Leu Ile Arg 375 Gly Ser Gin Ile Val1 455 Glu Gly Cys met Val 535 Val Ala Ile Arg Arg Leu Thr Thr 440 Ile Pro Phe Val1 Thr 520 Lys Arg Leu Gin Arg 600 Ser Giu Pro 425 Leu Ala Giu Leu Arg 505 Vai Gly Asp Arg Val1 585 Gly 395 Pro Ile Gin Gin Asn 475 Thr Pro Trp Leu Gin 555 Giu Glu Phe Leu Ser Giu 445 Giu Met Asn Leu Gin 525 Gly Leu Asn Ala Asp Pro Ala 430 Giu Leu Val Gly Phe 510 Tyr Pro Ala Asp Ile 590 Thr Thr 415 Arg Ala Gly Gin Trp, 495 G ly Ala Val Thr Leu 575 Arg <210> 229 <211> 603 <212> DNA <213> Corynebacteriun giutamicun <220> <221> CDS <222> (101)..(580) <223> FRXA02086 <400> 229 gatgatcagc cgctggctac cactgctgac caggttgcac tggcactgcg cgatgaaatt WO 01/00843 aacgatctca tcgaggctgg cgcgaagatc atccaggtgg atg agc ctg Met Ser Leu gtg Val tgg Trp gtC Val tcc Ser cgt Arg ctc Leu t cc Ser ga t Asp gga Gly aac Asn tcc Ser caa Gin tcg Ser tcc Ser ggc Gly gcg Al a cc t Pro tgg Trp 135 tgt Cys gtg Val a tc Ile gtc Val gac Asp gtc Val cag Gin cgc Arg 120 cca Pro tgc Cys gac Asp cac H-is atc Ile atg Met gga Giy aaa Lys cag Gin gaa Giu ccg Pro tcc Ser ac C Thr gcg Al a cag Gin cc t Pro gtg Val1 ctg Leu gtg Val cta Leu ttc Phe cac His ttg Leu gtc Val 75 gg t Gly gac Asp tgg Trp gaa Glu cga Arg cgc Arg atg Met gat Asp 60 ctc Leu gtg Val ggt Gly gtc Val gc t Ala 140 gac Asp ctg Leu tgc Cys 45 gcc Al a gc t Al a tgg Trp, ctc Leu aac Asn 125 tcc Ser aag cct Lys Pro gcc ggc Ala Gly gag ttc Giu Phe acc acc Thr Thr aaa tct Lys Ser 80 cac tcc His Ser gct gca Ala Ala tgt ggt Cys Gly gtt ctc Val Leu 145 gcc Ala gca Ala aac Asn atc Ile tcc Ser ccg Pro ctg Leu ctg Leu 130 gtt Val1 tac Tyr ccc Pro gaa Glu gaa Glu ggc Gly cgc Arg cag Gin 115 aag Lys gag Glu PCTIBOOIOO923 cga ttc 115 Arg Phe ctg cag 163 Leu Gin gac gac 211 Asp Asp gtg atc 259 Val Ilie gca gca 307 Ala Ala ttc gag 355 Phe Glu gtt cct 403 Val Pro 100 tcc gtg 451 Ser Val Acc cgt 499 Thr Arg tcc gct 547 Ser Ala aag cag gct cgt gag aaa atc gga gca act atc taaattgggt taccgctagg 600 LYS Gin Ala Arg Glu Lys Ile Gly Ala Thr Ile 150 155 160 aac 603 <210> 230 <211> 160 <212> PRT <213> Corynebacterium glutamicum <400> 230 Met Ser Leu Arg Phe Val Asn Cys Cys Pro Leu Arg Asp Val Asp Lys 1 5 10 Pro Ala Tyr Leu Gin Trp, Ser Val Asp Ser Phe Arg Leu Ala Thr Ala 25 Gly Ala Pro Asp Asp Val Gin Ile His Thr His Met Cys Tyr Ser Giu 40 WO 01/00843 WO 0100843PCT/EBOO/00923 Phe Thr Ser Ser Ala Giy Leu 145 Ser Arg Leu Ser Asp Gly Lys 150 Ser Ser Gly Ala Pro Trp 135 Gin Asp Ala Asp Leu 110 Pro Leu Ala <210> 231 <211> 1326 <212> DNA <213> Corynebacteriun glutamicui <220> <221> CDS <222> (101)..(1303) <223> RXN02648 <400> 231 atgaataaaa ttccgggtgc agtgaccgta ggtgaggtaa gagtttgata ctttctttcg acttttagat tggattttca atc Ile gat Asp ttc Phe gac Asp tcc Ser ggt Gly tct Ser tct Ser ctt Leu gg t Gly t tg Leu ggt Gly gat Asp gag Glu tgg Trp 339 acgcggttag agtcgaatga atg agc cag aac cgc Met Ser Gin Asn Arg 1 acc cca gag cta ctt Thr Pro Giu Leu Leu ggg gag gag gaa ttc Gly Giu Glu Glu Phe atc aag cgc cag gtt Ile Lys Arg Gin Val tac ggc cac gtc acc Tyr Giy His Val Thr tac tcc ttc acc cgc Tyr Ser Phe Thr Arg WO 01/00843 ctg ggc gga Leu Gly Gly gca gtg cgt Ala Val Arg cgt cgc gac Arg Arg Asp 120 ggc atc ttc Gly Ile Phe 135 cct att acc Pro Ile Thr 150 ctg aag aag Leu Lys Lys gca cta tcc Ala Leu Ser act gat gaa Thr Asp Glu 200 tac aag atc Tyr Lys Ile 215 gac ttg gca Asp Leu Ala 230 gat tac ttg Asp Tyr Leu gtg aag ggc Val Lys Gly tct tgg cac Ser Trp His 280 ggt gag atc 1027 Gly Giu Ile 295 tct cct cgt 1075 c tg Leu tcc Ser 105 cgc Arg acc Thr tac Tyr ggc Giy c ca Pro 185 gaa Giu atc Ile gaa Giu gac Asp ctt Leu 265 gga Gly c tg Leu cac ac c Thr acc Thr gca Ala ggt Gly att Ile atg Met 170 gga Gly gtc Val1 acc Thr gca Ala t gg Trp 250 cca Pro cca Pro cgc Arg gca 75 a t~g Met cct Pro t tg Leu cgc Arg ggC Gly 155 aac Asn tct Ser gtc Val gat Asp tgg Trp 235 atc Ile aag Lys cac His gca Ala cac acc Thr ggc Gly ttc Phe gc t Ala 140 cag Gin gca Ala gca Ala gca Ala gca Ala 220 gat Asp ggt Gly gaa Giu gtC Val1 gag Giu 300 gag gat Asp aac Asn agc Ser 125 tct Ser gaa Giu gcg Ala gc t Ala gca Ala 205 gg t Gly cag Gin ac a Thr cag Gin act Thr 285 gtc Val1 tgg acc Thr atc Ile 110 gaa Giu gtg Val gaa Giu gga Gly cga Arg 190 tgt Cys ctg Leu atc Ile cgC Arg ac c Thr 270 gac Asp ggt Giy cgt gac Asp 95 gag Giu gca Ala ggc Gly act Thr gc t Ala 175 t tg Leu gc t Ala acc Thr aac Asn atc Ile 255 cgc Arg atc Ile 9gc Gly gta 80 cgt Arg ctg Leu tac Tyr aac Asn cag Gin 160 ac c Thr ac c Thr ga t Asp gtt Val c ca Pro 240 ga t Asp c tg Leu cca Pro ttC Phe tgg tgg Trp acc Thr gag Giu cca Pro 145 acg Thr gac Asp aac Asn gcg Ala cag Gin 225 gag Giu gcc Ala cac Hius t tc Phe tcC Ser 305 gaa gca Ala agc Ser ga t Asp 130 gag Giu ga t Asp ggC Gly aag Lys ctt Leu 210 ctc Leu cca Pro atc Ile atc Ile ggt Gly 290 ttC Phe gaa agc Ser ttC Phe 115 cca Pro ttC Ph4 gtt Val1 ttc Phe ttC Phe 195 tcc Ser gac Asp agc Ser aac Asn tgc Cys 275 gac Asp gaa Giu a ac PCTIIBOO/00923 cag gaa 403 Gin Glu 100 tct gat 451 Ser Asp gta tct 499 Val Ser acc gga 547 Thr Gly gat ctg 595 Asp Leu 165 gtt gca 643 Val Ala 180 tac gac 691 Tyr Asp cag gaa 739 Gin Glu gca ccg 787 Ala Pro gtg aag 835 Val Lys 245 agt gca 883 Ser Ala 260 tgg ggc 931 Trp Gly atc att 979 Ile Ile ggc gca Giy Ala aag ctt WO 01/00843 Ser Pro Arg His Ala His Glu Trp 2 310 315 cct gaa ggc tct gtt atc tac cct 1123 Pro Giu Gly Ser Val Ile Tyr Pro 330 gct gtg gag cac cca cgc ctg gtt 1171 Ala Val Giu His Pro Arg Leu Val 2 345 aag ctt gtt ggc cct gag aac gtc 1219 Lys Leu Val Gly Pro Glu Asn Val 360 365 ggc gga cgt ctg cat tcc cag atc 1267 Gly Gly Arg Leu His Ser Gin Ile 2 375 380 gta gag ggc gct cgc att gca tca 1313 Val Glu Gly Ala Arg Ile Ala Ser 390 395 aacgagggtt gct 1326 <210> 232 <211> 401 <212> PRT <213> Corynebacterium glutamicum <400> 232 Met Ser Gin Asn Arg Ile Arg Thr 1 Thr Pro Giu Leu Leu Asp Ala Asn Gly Glu Giu Glu Phe Phe Gin Ile 1 40 Ile Lys Arg Gin Val Asp Leu Giy 55 Tyr Gly His Val Thr Ser Gly Ala 1 Tyr Ser Phe Thr Arg Leu Gly Giy I Trp Ala Ser Gin Glu Ala Val Arg 100 Thr Ser Phe Ser Asp Arg Arg Asp 2 115 120 Glu tct Ser atc Ile act Thr aag Lys 385 t tc Phe Giy Ser Ser Leu Gly Thr Gly Phe PCTIIBOO/00923 Giu Asn Lys Leu 325 cac tcc atc aac His Ser Ile Asn 340 gtt cag ttc gcc Val Gin Phe Ala 355 gac tgt ggt ctg Asp Cys Gly Leu 370 ctg gag tcc cta Leu Glu Ser Leu taagctagac Ser Asn Val1 Asn Ala Asp Asn Ser 125 WO 01/00843 WO 0100843PCTIiBOOIO0923 Giu Asp Pro Val Ser Gly Ile Phe Thr Gly Arg Ala Ser Val Gly Asn 130 135 140 Pro Giu Phe Thr Gly Pro Ile Thr Tyr Ile Gly Gin Giu Giu Thr Gin 145 150 155 160 Thr Asp Vai Asp Leu Leu Lys Lys Gly Met Asn Ala Ala Gly Ala Thr 165 170 175 Asp Gly Phe Val Ala Ala Leu Ser Pro Gly Ser Ala Ala Arg Leu Thr 180 185 190 Asn Lys Phe Tyr Asp Thr Asp Giu Giu Val Val Ala Ala Cys Ala Asp 195 200 205 Ala Leu Ser Gin Glu Tyr Lys Ile Ile Thr Asp Ala Gly Leu Thr Val 210 215 220 Gin Leu Asp Ala Pro Asp Leu Ala Giu Ala Trp Asp Gin Ile Asn Pro 225 230 235 240 Glu Pro Ser Val Lys Asp Tyr Leu Asp Trp Ile Gly Thr Arg Ile Asp 245 250 255 Ala Ile Asn Ser Ala Val Lys Gly Leu Pro Lys Giu Gin Thr Arg Leu 260 265 270 His Ile Cys Trp, Gly Ser Trp, His Giy Pro His Val Thr Asp Ile Pro 275 280 285 Phe Gly Asp Ile Ile Gly Giu Ile Leu Arg Ala Giu Val Gly Gly Phe 290 295 300 Ser Phe Giu Gly Ala Ser Pro Arg His Ala His Giu Trp Arg Vai Trp 305 310 315 320 Giu Giu Asn Lys Leu Pro Giu Gly Ser Val Ile Tyr Pro Gly Val Val 325 330 335 Ser His Ser Ile Asn Ala Vai Giu His Pro Arg Leu Val Ala Asp Arg 340 345 350 Ile Vai Gin Phe Ala Lys Leu Val Gly Pro Glu Asn Val Ile Ala Ser 355 360 365 Thr Asp Cys Gly Leu Gly Giy Arg Leu His Ser Gin Ile Ala Trp Ala 370 375 380 Lys Leu Glu Ser Leu Vai Giu Gly Ala Arg Ile Ala Ser Lys Giu Leu 385 390 395 400 Phe <210> 233 <211> 548 <212> DNA <213> Corynebacterium giutamicun WO 01/00843 WO 0100843PCTflIBOO/00923 <220> <221> CDS <222> <223> FRXA02648 <400> 233 gac gca ccg gac ttg Asp Ala Pro Asp Leu 1 5 gca gaa gca tgg Ala Giu Ala Trp cag atc aac cca Gin Ile Asn Pro gag cca Giu Pro agc gtg aag Ser Val Lys aac agt gca Asn Ser Ala tac ttg gac tgg Tyr Leu Asp Trp atc Ile ggt aca cgc atc Gly Thr Arg Ile gat gcc atc Asp Ala Ilie ctg cac atc Leu His Ile gtg aag ggc ctt Val Lys Gly Leu cca Pro aag gaa cag acc Lys Glu Gin Thr tgc tgg Cys Trp ggc tct tgg cac Giy Ser Trp His cca cac gtc act Pro His Val Thr atc cca ttc ggt Ile Pro Phe Giy gac Asp atc att ggt gag Ilie Ile Gly Giu ctg cgc gca gag Leu Arg Aia Glu ggt ggc ttc tcc Giy Gly Phe Ser 192 240 288 gaa ggc gca tct Giu Gly Aia Ser cgt cac gca cac Arg His Ala His tgg cgt gta tgg Trp Arg Val Trp gaa gaa Giu Giu aac aag ctt Asn Lys Leu tcc atc aac Ser Ilie Asn 115 gaa ggc tct gtt Glu Giy Ser Vai atc Ile 105 tac cct ggt gtt Tyr Pro Giy Vai gtg tct cac Vai Ser His 110 cgt atc gtt Arg Ile Vai gct gtg gag cac Ala Val Giu His cca Pro 120 cgc ctg gtt gct Arg Leu Val Aia cag ttc Gin Phe 130 gcc aag ctt gtt Aia Lys Leu Val 9gc Giy 135 cct gag aac gtc Pro Giu Asn Val gcg tcc act gac Aia Ser Thr Asp 432 480 tgt ggt ctg ggc gga cgt ctg cat tcc cag atc gca tgg gca aag ctg Cys Gly Leu Gly Gly Arg Leu His Ser Gin Ile Aia Trp Ala Lys Leu 145 150 i55 160 gag tcc cta gta Giu Ser Leu Val gag Glu 165 ggc gct cgc att Giy Ala Arg Ilie tca aag gaa ctg Ser Lys Giu Leu ttc Phe 175 taagctagac aacgagggtt gct 548 <210> 234 <211> 175 <212> PRT <213> Corynebacterium giutamicum <400> 234 Asp Ala Pro Asp Leu Ala Giu Ala Trp Asp Gin Ile Asn Pro Giu Pro WO 01/00843 WO 0100843PCTIBOOIOO923 Ser Asn Cys Asp Giu Asn Ser Gin Cys 145 Glu Tyr Lys Trp Giu Pro Giu Val1 Leu Gly Giu 165 Asp Leu Gly Leu His Ser His Gly 135 Leu Ala Trp, Pro Pro Arg Ala Val1 Pro 120 Pro His Arg Asp Leu Pro Phe Trp Val1 110 Arg Ser Ala Leu Ala His Phe Ser Glu Ser Ile Thr Lys Phe 175 <210> 235 <211> 784 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(784) <223> FRXA02658 <400> 235 atgaataaaa ttccgggtgc agtgaccgta ggtgaggtaa gagtttgata ctttctttcg acttttagat tggattttca atc agg acc act cac gtt ggt tcc ttg ccc cgt Ile Arg Thr Thr His Val Gly Ser Leu Pro Arg 15 gat gca aac atc aag cgt tct aac ggt gag att Asp Ala Asn Ile Lys Arg Ser Asn Gly Giu Ile 30 ttc cag att ctg cagtct tct gta gat gac gtg Phe Gin Ile Leu Gin Ser Ser Vai Asp Asp Val 45 acgcggttag agtcgaatga atg agc cag aac cgc 115 Met Ser Gin Asn Arg acc cca gag cta ctt Thr Pro Giu Leu Leu ggg gag gag gaa ttc Gly Giu Giu Giu Phe atc aag cgc cag gtt Ile Lys Arg Gin Vai WO 01/00843 gac ctg ggt Asp Leu Gly tcc ggt gca Ser Gly Ala ctg ggc gga Leu Gly Gly gca gtg cgt Ala Val Arg cgt cgc gac Arg Arg Asp 120 ggc atc ttc Gly Ile Phe 135 cct att acc Pro Ile Thr 150 ctg aag aag Leu Lys Lys gca cta tcc Ala Leu Ser act gat gaa Thr Asp Glu 200 atc Ile gt t Val1 ctg Leu tcc Ser 105 cgc Arg ac c Thr tac Tyr ggc Gly cca Pro 185 gaa Giu gac Asp gac Asp acc Thr acc Thr gca Ala ggt Gly att Ile atg Met 170 gga Gly gtc Val gaa Giu aac Asn 80 cgt Arg ctg Leu t ac Tyr aac Asn cag Gin 160 acc Thr acc Thr ga t Asp tac Tyr tac Tyr tgg Trp ac c Thr gag Glu cca Pro 145 acg Thr gac Asp aac Asn gcg Ala ggc Gly tcc Ser gca Ala agc Ser ga t Asp 130 gag Glu gat Asp 9gc Gly aag Lys ctt Leu 210 PCTIIBOO/00923 gtc acc 307 Val Thr acc cgc 355 Thr Arg cag gaa 403 Gin Giu 100 tct gat 451 Ser Asp gta tct 499 Val Ser acc gga 547 Thr Gly gat ctg 595 Asp Leu 165 gtt gca 643 Val Ala 180 tac gac 691 Tyr Asp cag gaa 739 Gin Giu tac aag atc atc acc gat gca. ctg acc gtt cag ctc gac gca Tyr Lys Ile Ile Thr Asp Ala Gly Leu Thr Val Gin Leu Asp Ala 215 220 225 <210> 236 <211> 228 <212> PRT <213> Corynebacterium glutamicuin <400> 236 Met Ser Gin Asn Arg Ile Arg Thr Thr His Val Gly Ser Leu Pro Arg 1 5 10 Thr Pro Giu Leu Leu Asp Ala Asn Ile Lys Arg Ser Asn Gly Giu Ile 25 Gly Giu Glu Giu Phe Phe Gin Ile Leu Gin Ser Ser Val Asp Asp Val 40 Ile Lys Arg Gin Val Asp Leu Gly Ile Asp Ile Leu Asn Giu Gly Giu 345 WO 01/00843 PCT/IBOO/00923 55 Tyr Gly His Val Thr Ser Gly Ala Val Asp Phe Gly Ala Trp Trp Asn 70 75 Tyr Ser Phe Thr Arg Leu Giy Gly Leu Thr Met Thr Asp Thr Asp Arg 90 Trp Ala Ser Gin Giu Ala Val Arg Ser Thr Pro Gly Asn Ile Glu Leu 100 105 110 Thr Ser Phe Ser Asp Arg Arg Asp Arg Ala Leu Phe Ser Giu Ala Tyr 115 120 125 Glu Asp Pro Val Ser Gly Ile Phe Thr Gly Arg Ala Ser Val Gly Asn 130 135 140 Pro Giu Phe Thr Gly Pro Ile Thr Tyr Ile Gly Gin Glu Giu Thr Gin 145 150 155 160 Thr Asp Val Asp Leu Leu Lys Lys Gly Met Asn Ala Ala Gly Ala Thr 165 170 175 Asp Gly Phe Val Ala Ala Leu Ser Pro Gly Ser Ala Ala Arg Leu Thr 180 185 190 Asn Lys Phe Tyr Asp Thr Asp Glu Giu Val Val Ala Ala Cys Ala Asp 195 200 205 Ala Leu Ser Gin Giu Tyr Lys Ile Ile Thr Asp Ala Gly Leu Thr Val 210 215 220 Gin Leu Asp Ala 225 <210> 237 <211> 408 <212> DNA <213> Corynebacteriui giutamicun <2 <221> CDS <222> (101)..(385) <223> RXC02238 <400> 237 ggcgcttagc caaaacatag agcggtaggg tatgcttatc cgattgagca acctttcccg ctcttaacac tactgtccat atacttttga aaaggtgtca gtg acc aac gtg agc 115 Val Thr Asn Val Ser 1 aac gag acc aac gcc acc aag gcc gtc ttc gat ccg cca gtg ggc att 163 Asn Giu Thr Asn Ala Thr Lys Ala Val Phe Asp Pro Pro Val Gly Ile 15 acc gct cct ccg atc gat gaa ctg ctg gat aag gtc act tcc aag tac 211 Thr Ala Pro Pro Ie Asp Giu Leu Leu Asp Lys Val Thr Ser Lys Tyr 30 WO 01/00843 gcc ctc gtg atc ttc gca gcc aag cgt Ala Leu Val Ilie Phe Ala Ala Lys Arg 45 tac cat cag gca gat gag gga gta ttc Tyr His Gin Ala Asp Glu Gly Val Phe 60 act ccg cag cca ggc gaa aag cca ctt Thr Pro Gin Pro Giy Glu Lys Pro Leu 75 aat gca ggt ctg ttg gac cac gag gaa Asn Ala Gly Leu Leu Asp His Giu Glu cac <210> 238 <211> <212> PRT <213> Corynebacterium giutamicum <400> 238 Val Thr Asn Val Ser Asn Glu Thr Asn 1 Pro Pro Val Gly Ile Thr Ala Pro Pro Val Thr Ser Lys Tyr Ala Leu Val Ile Gin Ilie Asn Ser Phe Tyr His Gin Ala 55 Ile Gly Pro Leu Val Thr Pro Gin Pro Ala Leu Arg Giu Ile Asn Ala Gly Leu PCT/1B00100923 cgc cag atc aac agc ttc 259 Arg Gin Ile Asn Ser Phe ttc atc gga cca ttg gtt 307 Phe Ile Gly Pro Leu Val att gct ctg cgt gag atc 355 Ile Ala Leu Arg Giu Ile 80 taaaagacct tataacttca 405 Lys Glu Ala Gly Lys His <210> 239 <211> 1827 <212>. DNA <213> Corynebacterium giutarnicum <220> <221> CDS <222> (101)..(1804) <223> RXCO0128 <400> 239 ccattttccg tttggtcttg cctaaagaac cgcatggaaa ttatcgtgaa gcaccgatcc cgttgatcgc tccagagaca ccgtgggaag gggagcagca gtg agt aaa att tcg 115 Val Ser Lys Ile Ser 1 WO 01/00843 acg aaa ctg aag gcc ctc acc gcg gtg ctg tct gtg acc act Thr Lys Leu Lys Ala Leu Thr Ala Val Leu Ser Val Thr Thr 1s PCT/IBOO/00923 gc t Ala t ca Ser aat Asn tat Tyr acg Thr att Ile gcg Ala cag Gln gat Asp 150 aga Arg gat Asp gag Giu cc t Pro gcg Ala 230 ggg Gly ttt Phe caa Gin ccg Pro cgc Arg ga t Asp atc Ile ccg Pro 135 ggg Gly aac Asn cc t Pro tcg S er t cg Ser 215 t cg Ser tgt Cys tcc Ser gat Asp act Thr agc Ser ctg Leu cgt Arg 120 gag Giu gag Giu ga t Asp tct Ser cag Gin 200 ccg Pro ttc Phe tcc Ser ggg Gly ccg Pro cag Gin acg Thr aac Asn 105 gga Giy aa t Asn tgg Trp ctg Leu ggC Gly 185 tcg Ser gca Ala gtg Val1 cag Gin aca Thr 45 atc Ile gcg Ala gc t Ala ggt Gly gga Gly 125 gaa Giu gc t Ala t ac Tyr gtg Vai gtg Val 205 ggt Gly ga t Asp aac Asn 30 c aa Gin cgc Arg gcg Ala gcg Ala tcg Ser 110 acg Thr gc t Ala ttg Leu act Thr 99g Gly 190 ctg Leu gtg Val1 999 Gly acg Thr gag Giu Gly aag Lys tcg Ser acg Thr ttg Leu gag Giu ccg Pro ccg Pro 175 gat Asp atg Met gtc Val1 gag Giu gat Asp ata Ile t tc Phe gcg Ala act Thr aa t Asn ctc Leu atc Ile gac Asp 160 cac His C99 Arg gcc Ala aat Asn tat Tyr 240 ccg caa Pro Gin gca ggg Ala Giy ttc agc Phe Ser tat ctg Tyr Leu cgt att Arg Ile gcg gaa Ala Giu agc ggt Ser Gly 130 acg atg Thr Met 145 ggg att Gly Ile gat gtg Asp Val cgt tgg Arg Trp ctt ctg Leu Leu 210 cag ctg Gin Leu 225 cag ttc Gin Phe gtg Val1 ccg Pro gc a Ala acg Thr ttg Leu cga Arg 115 9gC Gly cgt Arg tta Leu tat Tyr ttg Leu 195 gtt Val1 t cc Ser act Thr 163 211 259 307 355 403 451 499 547 595 643 691 739 787 835 883 gga'aat ttg gat gat gat gcg cgt ttg cgt ttc gcc gcc cag gcc gtg WO 01/00843 WO 0100843PCT/IBOO/00923 Giy Asn Leu Asp Asp 250 Asp Ala Arg Leu Phe Ala Ala Gin Ala Val 260 gtc gct Val Ala tgg acg Trp Thr gac ggc Asp Gly ttg Leu gcg Ala 265 cat gct gat gtc His Ala Asp Val ccc tac act Pro Tyr Thr ttg Leu 275 ctc gcc 1027 Leu Ala 295 ttt gcg 1075 Phe Ala 310 agt cca 1123 Ser Pro att tcc 1171 Ile Ser gag gca 1219 Giu Ala ttg agg 1267 Leu Arg 375 ggg ttg 1315 Giy Leu 390 tcg gca 1363 Ser Ala cca agg 1411 Pro Arg gcg ccg ttg ctg Ala Pro Leu Leu 280 gaa tac aac cca Glu Tyr Asn Pro ttg cag gat gga Leu Gin Asp Gly 315 cta cag ggc att Leu Gin Gly Ile 330 tcc tcc gcc aat Ser Ser Ala Asn cca acg ctc Pro Thr Leu acc Thr 290 tac acc aac acg gtg Tyr Thr Asn Thr Val 305 tcg agg gtc agt tcc Ser Arg Val Ser Ser acc gat gac Thr Asp Asp tcc acg ttg Ser Thr Leu ggc aat gtg Gly Asn Val 325 979 tcg ttg Ser Leu tgg agc ggt gga Trp Ser Gly Giy 320 gat atc gat tct gca gcg Asp Ile Asp Ser Ala Ala 340 gta cgc cac gaa aac aac Val Arg His Glu Asn Asn 355 gtg gtg gca Val Val Ala 345 gtg ctt Val Leu act gtt ggc tcc Thr Val Gly Ser 365 350 atg gaa ggc gtg act tca gat gcg Met Glu Gly Val Thr Ser Asp Ala 370 ccc acc ttt gaa tac gcg tcg agt Pro Thr Phe Giu Tyr Ala Ser Ser 385 gaa acg atc act cgt Glu Thr Ile Thr Arg 380 tgg gct gtg gtg Trp Ala Val Val 395 aca acc ggt gag Thr Thr Gly Giu 410 gat gtg acg ggt Asp Val Thr Gly gat ggg Asp Gly gag acg Giu Thr ctc gtc cag acg Leu Val Gin Thr 415 ccg atc tct gaa Pro Ile Ser Giu 430 cct gtc cga gtc gca cga Pro Vai Arg Val Ala Arg 400 405 gag gcg gag att gtg ctg Giu Ala Glu Ile Val Leu 420 ttc caa ctg tca cga act Phe Gin Leu Ser Arg Thr 435 aag gtg tac gtg ggc gtc Lys Val Tyr Val Gly Val 450 425 cgg gcc ggg gtc 1459 gcc atg atc att gaa ggc Giu Giy Giy Val Arg Ala Ala Met Ile Ile WO 01/00843 WO 0100843PCT/IBOO/00923 gta acg 1507 Val Thr 455 gtg gcg 1555 Val Ala 470 gac ggc 1603 Asp Gly cgc gtc 1651 Arg Val ctc agc 1699 Leu Ser cgt cct ggt ccg ggc Arg Pro Gly Pro Gly 460 ccg agc ttg ggc gag Pro Ser Leu Gly Glu 475 gag cgg cgc gtg aca aat atc acg gag Giu Arg Arg Val Thr Asn Ile Thr Giu 465 gcg gcg ctg tcg atc aac tgg cgc cca Ala Ala Leu Ser Ile Asn Trp Arg Pro att ttg Ile Leu gag cag Glu Gin 505 gcg ccg Ala Pro ctt gtg ggc acg tca att Leu Val Giy Thr Ser Ile 490 495 gac gga tcg gcg att tcg Asp Giy Ser Ala Ile Ser 510 gtg gtg gcg gtg gca agt Val Val Ala Val Ala Ser 525 cat gcg atg ctt cag ctg His Ala Met Leu Gin Leu gag acg ccg ctg Giu Thr Pro Leu 500 tcg atg ccg agc ggg-aat Ser Met Pro Ser Gly Asn 515 tcc gcg acg acg gtc tac Ser Ala Thr Thr Vai Tyr 530 ccg act gcc gat aat gat Pro Thr Ala Asp Asn Asp gtc act gat 1747 Vai Thr Asp 535 att tgg cgc 1795 Ile Trp Arg 550 gtt gcg tac 1827 Vai Ala Tyr tcg 540 gag gtg ccc ggt Glu Val Pro Gly ttg Leu 545 cig ggc acg cgt Leu Gly Thr Arg 560 gcg gcg ccg gtg Ala Ala Pro Val 565 tgatggagct gttcttcccg cgc <210> 240 <211> 568 <212> PRT <213> Corynebacterium glutanicun <400> 240 Val Ser Lys Ile Ser Thr Lys Leu 1 5 Val Thr Thr Leu Val Aia Gly Cys Pro Gin Vai Leu Arg Ser Phe Ser Aia Gly Pro Thr Pro Asn Gin Asp Phe Ser Ala Gly Ala Tyr Pro Thr 70 ys Ala Leu Thr Ala Val Leu Ser Ser Thr Leu Pro Gin 31y Ser Gin Ser Asn Thr Asp Gin Giu Ile Arg Giy Phe Pro Asp Leu 1ln Gin Tyr Ala Ala Lys WO 01/00843 WO 0100843PCT[IB00100923 Tyr Leu Thr Giu Gly Thr Arg Ser Thr Trp Asn Pro Ala Ala Ser Thr 90 Arg Ala Ser Thr 145 Gly Asp Arg Leu Gin 225 Gin Ala Tyr Leu Thr 305 Ser Ile Arg Vai Glu 385 Arg Ile Tyr Glu Giu 165 Phe Asn Gly Asp Leu 245 Val1 Ala Asp Leu Val1 325 Ala Asn Ala Ser Ile Ala Gin Asp 150 Arg Asp Glu Pro Ala 230 Gly Trp Asp Leu Phe 310 Ser Ile Glu Leu Gly 390 Leu Gin Glu Ile 155 Asn Val1 Ser Ser Phe 235 Asp Ala Leu Pro Gly 315 Ile Asn Val1 Ile Val 395 Pro Val1 Phe 140 Asp His Leu Thr Pro 220 Asn Ala Asp Ser Glu 300 Ser Trp Val Gly Thr 380 Asp Gly Gly 125 Glu Ala Tyr Val1 Val1 205 Gly Asp Arg Val1 Giu 285 Ala Leu Ser Val1 S er 365 Arg G ly Ser 110 Thr Ala Leu Thr Gly 190 Leu Val1 G ly Leu Ala 270 Phe Tyr Ser Gly Ala 350 Met Pro Glu Asn Leu Ile Asp 160 His Arg Ala Asn Tyr 240 Phe Pro Thr Asn Val 320 Asp Val Gly Phe Pro 400 WO 01/00843 Val Arg Val Ala Giu Ile Gin Leu Ser 435 Val Tyr Val 450 Thr Asn Ile 465 Ile Asn Trp Giu Thr Pro Met Pro Ser 515 Ala Thr Thr 530 Thr Ala Asp 545 Arg Ala Ala Giu Gly Met Pro Gly 475 Val Gly Val1 Ala Pro 555 Leu Pro Ile Giy 460 Giu Gly Ser Ala Met 540 Gly PCTJIBOO/00923 -ir Giu Lu Phe Ly Lys r-g Val ~u Ser 480 1e Pro e r Ser au Pro Ly Thr 560 <210> 241 <211> 1344 <212> DNA <213> Corynebacteriuni giutamicun <22 0> <221> CDS <222> (101)..(1321) <223> RXA02240 <400> 241 cagctagacc actgacattg cagttttaga cagcttggtc aagactattt attctcaact tcttcgaaag aagggtattt gcc gtc cgt ttg ttc acc agt gaa tct gta act Ala Val Arg Leu Phe Thr Ser Glu Ser Val Thr 15 aaa ata tgt gat gct att tcc gat acc att ttg Lys Ilie Cys Asp Ala Ilie Ser Asp Thr Ile Leu 30 aaa gat ccg cag tcg cgc gtc gca gtg gaa act Lys Asp Pro Gin Ser Arg Val Ala Val Giu Thr tatattggtt ttttgtattt gtg gct cag cca acc 115 Val Ala Gin Pro Thr 1 gag gga cat cca gac 163 Glu Gly His Pro Asp gac gcg ctg ctc gaa 211 Asp Ala Leu Leu Glu gtg gtc acc acc gga 259 Val Val Thr Thr Gly WO 01/00843 WO 0100843PCTIiBOO/00923 atc gtc Ile Val cat gtt gtt ggc His Val Val Gly gtc cgt acc agc Vai Arg Thr Ser tac gta gag atc Tyr Val Giu Ile cct Pro caa tta gtc cgc Gin Leu Val Arg aag ctc atc gaa Lys Leu Ile Giu atc Ile gga ttc aac tcc Giy Phe Asn Ser gag gtt gga ttc Giu Val Giy Phe gga cgc acc tgt Gly Arg Thr Cys gtc tca gta tcc Vai Ser Val Ser atc ggt Ile Giy 100 gag cag tcc Giu Gin Ser cag Gin 105 gaa atc gct gac Giu Ilie Aia Asp gtg gat aac tcc gac gaa gcc Vai Asp Asn Ser Asp Giu Aia 115 cgc acc aac ggc gac gtt gaa gaa gac gac cgc gca ggt gct ggc gac Arg Thr Asn Giy Asp Vai Giu Giu Asp Asp Arg Aia Giy Ala Giy Asp 120 *i25 130 cag ggc Gin Giy 135 ctg atg ttc ggc tac gcc acc aac gaa Leu Met Phe Gly Tyr Ala Thr Asn Giu 140 acc Thr 145 gaa gag tac atg Giu Giu Tyr met cct Pro 150 ctt cct atc gcg Leu Pro Ilie Ala gcg cac cga ctg Aia His Arg Leu tca Ser 160 cgt cgt ctg acc Arg Arg Leu Thr gtt cgt aaa gag Vai Arg Lys Giu atc gtt cct cac Ile Val Pro His cgt cca gac gga.

Arg Pro Asp Gly aaa acc Lys Thr 180 cag gtc acc ttc gca tac gat gcg Gin Val Thr Phe Ala Tyr Asp Ala 185 gac cgc cct agc Asp Arg Pro Ser cac ctg gat His Leu Asp 195 cgt gca tgg Arg Ala Trp acc gtt gtc Thr Val Vai 200 atc tcc acc cag Ile Ser Thr Gin cac His 205 gac cca gaa gtt Asp Pro Giu Val gac Asp 210 ttg gaa Leu Giu 215 acc caa ctg cgc Thr Gin Leu Arg cac gtc att gat His Val Ile Asp tgg Trp 225 gta atc aaa gac Vai Ile Lys Asp gca Ala 230 ggc att gag gat ctg gca acc ggt gag Giy Ile Giu Asp Leu Ala Thr Gly Giu 235 acc gtg ttg atc Thr Val Leu Ile cct tca ggt tcc Pro Ser Giy Ser t tc Phe 250 att ctg ggt ggc ccc atg ggt gat gcg Ile Leu Gly Giy Pro Met Gly Asp Ala 255 ggt ctg Gly Leu 260 acc ggc cgc Thr Gly Arg ggt ggt gga Gly Gly Gly 280 aag Lys 265 atc atc gtg gat Ile Ile Val Asp acc Thr 270 tac ggt ggc atg Tyr Gly Gly.Met gct cgc cat Aia Arg His 275 gac cgc tct Asp Arg Ser gca ttc tcc ggt aag gat cca agc Ala Phe Ser Gly Lys Asp Pro Ser 285 aag gtg Lys Vai 290 WO 01/00843 WO 0100843PCTAIBOO/00923 gct gca tac gcc atg cgt tgg 1027 Ala Ala Tyr Ala Met Arg Trp 295 300 ctt gct gat cgc gct gaa gtt 1075 Leu Ala Asp Arg Ala Glu Val 310 315 aag cca gtc gga ctt tac gtt 1123 Lys Pro Val Gly Leu Tyr Val gta gca aag aac atc gtg gca gca ggC Val Ala Lys Asn Ile Val Ala Ala Gly 305 cag gtt gca tac gcc att gga cgc gca Gin Val Ala Tyr Ala Ile Gly Arg Ala 320 gaa acc ttt Glu Thr Phe gac Asp 325 acc aac aag gaa ggc Thr Asn Lys Glu Gly 340 ctg agc 1171 Leu Ser cgt cca 1219 Arg Pro gct gac 1267 Ala Asp 375 cct tgg 1315 Pro Trp 390 ttg gcc 1344 Leu Ala gac gag Asp Giu 345 gca gca Ala Ala att cag gct gcc Ile Gin Ala Ala 350 atc cgt gag ctt Ile Arg Giu Leu 335 at t Ile gtg ttg gag gtc ttt gac ctg Val Leu Glu Val Phe Asp Leu 355 gat ctg ctt cgt ccg atc tac Asp Leu Leu Arg Pro Ile Tyr 370 ggt cgc act gat ttg gac ctt Gly Arg Thr Asp Leu Asp Leu 360 365 act gct gcc tac ggc Thr Ala Ala Tyr Gly 380 gag gct atc gac cgc Glu Ala Ile Asp Arg 395 cac ttt His Phe 385 gtt gat gaa ctt cgc Val Asp Giu Leu Arg 400 gca gcc ctc aag Ala Ala Leu Lys 405 taaaaatctg atgtagtatc ttc <210> 242 <211> 407 <212> PRT <213> Corynebacterium glutamicum <400> 242 Val Ala Gin Pro Thr Ala Val Arg Leu Phe Thr Ser 1 5 10 Giu Gly His Pro Asp Lys Ile Cys Asp Ala Ile Ser 25 Asp Ala Leu Leu Giu Lys Asp Pro Gin Ser Arg Val 40 Val Val Thr Thr Gly Ile Val His Val Val Gly Giu 55 Ala Tyr Val Glu Ile Pro Gin Leu Val Arg Asn Lys Giu Ser Val1 Thr Asp Thr Ile Leu Ala Val Glu Thr Val Arg Thr Ser Leu Ile Glu Ile WO 01/00843 Gly Phe Asn Ser Val Ser Asri Ser Asp 115 Ala Gly Ala 130 Thr Glu Giu 145 Arg Arg Leu Pro Asp Gly Pro Ser His 195 Val Asp Arg 210 Trp, Val Ile 225 Thr Val Leu Gly Asp Ala Gly Met Ala 275 Lys Val Asp 290 Ile Val Ala 305 Ala Ile Gly Thr Asn Lys Glu Val Phe 355 Leu Arg Pro 370 70 Giu Glu Arg Gin Pro 150 Val Gin Thr Leu Ala 230 Pro Thr Gly Ala Leu 310 Lys Leu Arg Ala Vai Gin Thr Gly 135 Leu Arg Val Val1 Glu 215 Gly Ser Gly Gly Ala 295 Ala Pro Ser Pro Asp 375 Thr Asp Glu 125 Ala His Pro Al a His 205 His Thr Gly Asp Lys 285 Val1 Gin Giu Ala Giu 365 His Val1 Cys Gly 110 Asp Thr Arg His Gin 190 Asp Val1 Gly Gly Thr 270 Asp Ala Vai Thr Ala 350 Leu Phe Asp PCTIIBOO/00923 Giy Val Val Asp Asp Arg Asn Glu Leu Ser 160 Leu Arg 175 Asp Arg Pro Giu Ile Asp Giu Ile 240 Pro Met 255 Tyr Gly Pro Ser Lys Asn Ala Tyr 320 Phe Asp 335 Val Leu Asp Leu Gly Arg Giu Leu 400 Thr Asp Leu Asp Leu Pro Trp Giu WO 01/00843 WO 0100843PCTIIBOO/00923 Arg Ala Ala Leu Lys Leu Ala 405 <210> <211> <212> <213> <220> <221> <222> <223> 243 669

DNA

Corynebacterium glutamicum

CDS

(101)..(646) RXA0 0780 <400> 243 ccttttaagt ccttCtttgC ccgtgaataa ttctctggat agtttccacg tgcagttaag tcacgctgtt agacttgcct gcatgctctc gacaataaaa aeg Met 1 atc cgt gaa gat Ile Arg Glu Asp ctc gca aac gct Leu Ala Asn Ala gaa cac gat cca Glu His Asp Pro gcc cga ggc gat Ala Arg Giy Asp tta gaa Leu Giu aac gca gtg Asn Ala Val tac tcc gga ctc Tyr Ser Gly Leu gcc atc egg gca Ala Ile Trp Ala cat cga gtt His Arg Val cgc gta tea Arg Val Leu gcc aac agc tgg tgg aaa tcc Ala Asn Ser Trp Trp Lys Ser gcc caa tcC acc cga etc cc Ala Gin Phe Thr Arg Phe Leu etc cgc ggc ccc Phe Arg Giy Pro acc ggc act gaa Thr Gly Ile Giu cac ccc ggt gcc His Pro Gly Ala acc Thr ate gge cgt cgc Ile Gly Arg Arg tee Phe tt ate gac cac Phe Ile Asp His aeg gga aec gec Met Gly Ile Val 211 259 307 355 403 451 499 ggc gaa acc gct Gly Glu Thr Ala aec ggc gaa ggc gtc aeg ccc eac cac Ile Gly Giu Gly Val Met Leu Tyr His ggc gtc Gly Val 100 acc ccc ggc Thr Leu Giy cag gtt cec acc Gin Val Leu Thr acc aag cgc cac Thr Lys Arg His ccc acg cec Pro Thr Leu 115 tgc gac aac geg aca gtc ggc gcg ggc gca aaa aec tea ggt ccc atc Cys Asp Asn Vai Thr Val Gly Aia Gly Ala LysIle Leu Giy Pro Ile 120 125 130 acc atc ggc gaa ggc Ccc Thr Ile Gly Giu Gly Ser gca Ala 140 ate ggc gcc aat gca get gtc acc aaa Ile Giy Ala Asn Ala Val Val Thr Lys gac Asp 150 geg ccg gca gaa cac aec gca gec gga Val Pro Ala Giu His Ile Ala Val Gly 155 ate Ile 160 ccc gcg gta gca Pro Aia Val Aia WO 01/00843 WO 0100843PCTA[BOO/00923 cca cgt ggc aag aca gag aag atc aag ctc gtc gat ccg gac tat tac 643 Pro Arg Gly Lys Thr Glu Lys Ile Lys Leu Val Asp Pro Asp Tyr Tyr 170 175 180 att taagaacagt tagcgcccta cct 669 Ile <210> 244 <211> 182 <212> PRT <213> Corynebacterium glutanicun <400> 244 Met Ile Arg Glu Asp Leu Ala Asn Ala 1 Arg Trp Pro Ilie Met Leu Arg Ile Ala 145 Pro Gly Ala Ala His Gly Tyr His Leu 130 Val Ala Asp His Arg Pro Ile His Pro 115 Gly Val1 Val1 Glu Val1 Leu Ala Ile Val1 Leu Ile Lys Arg 165 Tyr Asn Ala Ala Thr 70 Gly Thr Cys Thr Asp 150 Pro Ile Val1 Ser Phe Gly Thr Gly Asn 120 Gly Pro Gly Glu Ser Lys Phe Phe Ile Val1 Val1 Ser His 155 Glu His Gly Ser Leu Phe Gly Leu Gly Ala 140 Ile Lys ASP Pro ASP <210> 245 <211> 1056 <212> DNA <213> Corynebacterium giutamicun <2 <221> CDS <222> (101)..(1033) WO 01/00843 <223> RXA00779 <400> 245 cagaggccac ttcaagtaga tgtttcgtaa ttgtttacag PCTIIBOO/00923 cgtttacgca agcggtcgac caacaaaaac agcacttcaa tgattggagc accacccgac atg ggc aat gtg tac Met Gly Asn Val Tyr 115 aac Asn aag Lys ttC Phe gaa Glu gaa Glu gc a Ala gag Glu cca Pro gtc Val gca Ala 150 gac Asp gga Gly gta Val1 aac Asn C tC Leu aac Asn gat Asp gca Ala cgC Arg cgt Arg ggt Gly gc t Ala 135 aac Asn acc Thr acc Thr cag Gln atc I le acc Thr cca Pro gca Ala ac c Thr gga Gly cgc Arg gca Ala 120 gaa Glu cca Pro gac Asp gtc Val1 gtc Val 200 ggc Gly gca Ala aag Lys 45 gca Ala gg t Gly ctc Leu gc t Ala ggt Gly 125 gtc Val gac Asp atc Ile cag Glri cca Pro 205 cac Hius act Thr gac Asp ctg Leu atc Ile acc Thr tac Tyr 110 gca Ala ctt Leu acc Thr t tc Phe gtc Val1 190 gaa Glu acc cca ccg gca aag ccg aac Thr Pro Leu Val Lys Leu Asn gtc ctg gtc aag ctt gag tca Val Leu Val Lys Leu Glu Ser cgt atc ggt ctg gcc atc gtt Arg Ile Gly Leu Ala Ile Val aag cca ggc ggc acc atc gtt Lys Pro Gly Gly Thr Ile Val gca ctg gca atg gtc ggc gct Ala Leu Ala Met Val Gly Ala 80 atg ccg gag acc atg tcc aac Met Pro Glu Thr Met Ser Asn 100 ggt gca gag atc gtt ctt acc Gly Ala Glu Ile Val Leu Thr 115 aag gac aag gca gac gaa atc Lys Asp Lys Ala Asp Glu Ile 130 gct cgc cag ttc gag aac gag Ala Arg Gln Phe Glu Asn Glu 145 acc gcg aag gaa atc ctc gaa Thr Ala Lys Glu Ile Leu Glu 160 165 gtt gca agc ttc ggc acc ggc Val Ala Ser Phe Gly Thr Gly 175 180 ctg aag gaa aac aac gca gac Leu Lys Glu Asn Asn Ala Asp 195 gcg tcc cca ctt ctg acc gct Ala Ser Pro Leu Leu Thr Ala 210 163 211 259 307 355 403 451 499 547 595 643 691 739 WO 01/008.

ggc aag Gly Lys 215 ccc gag Pro Glu 230 aac gaa Asn Glu atc ctc Ile Leu ctt gca Leu Ala acc gac 1027 Thr Asp 295 cgc gac 1056 Arg Asp 310 43 gc Al gt Va atc Ile gtt Val1 gac gca atc gca ttc tcc c Asp Ala Ile Ala Phe Ser A 250 ggc ggt atc tcc acc ggc g Gly Gly Ile Ser Thr Gly A 265 2 gca aag cca gag aac gct g Ala Lys Pro Glu Asn Ala G 280 285 ttc ggc gag cgc tac gtc t Phe Gly Glu Arg Tyr Val S 300 taattcttag cgactgttaa cca gc rg ca la 70 gc ly cc er PCTABOO/00923 aac ttc atc 787 Asn Phe Ile acc gtc tcc 835 Thr Val Ser 245 gaa gag ggc 883 Glu Glu Gly 260 gct ctt gac 931 Ala Leu Asp 275 acc gtt gtc 979 Thr Val Val gaa gac atc Giu Asp Ile <210> 246 <211> 311 <212> PRT <213> corynebacteriun giutamicum <400> 246 Met Gly Asn Val Tyr Asn Asn Ilie I) 1 5 Leu Vai Lys Leu Asn Lys Leu ThrC Val Lys Leu Giu Ser Phe Asn Pro 1 Gly Leu Ala Ile Val Glu Asp Ala Gly Gly Thr Ile Val Glu Ala Thr 70 Ala Met Val Gly Ala Ala Arg Gly 'J Glu Thr Met Ser Asn Glu Arg Arg N 100 1 Glu Ile Val Leu Thr Pro Gly Ala 1 115 120 Thr Val Arg Lys Ala Met Gly Lys Pro Leu Ile Pro Leu Pro Ala Asp WO 01/00843 Lys Ala Asp 130 PCTIiBOO/00923 Leu Ala Arg Giu Ilie Val Ala Glu Arg Giu Asn Ala Gin 145 Lys Ser Giu Pro Gly 225 Val1 Ala Lys Ile Leu 305 Asn Leu Thr 180 Ala Thr Phe Val1 Giu 260 Leu Val1 Asp Giu Giu 165 Gly Asp Ala Ile Ser 245 Gly Asp Val Ile 135 Asn Thr Thr Gin Lys 215 Giu Glu Leu Ala Asp 295 Asp 140 Asp Ile Gin Pro 205 Ile Val1 Ser Gly Al a 285 Val1 Thr Phe Val 190 Giu Gin Leu Arg Ala 270 Gly Ser <210> 247 <211> 623 <212> DNA <213> Corynebacteriun giutainicum <220> <221> CDS <222> <223> RXN00402 <400> 247 act gac gaa aag gat gga aag cca gta ttg ccc tac ttc gtc act cca Thr Asp Giu Lys Asp Gly Lys Pro Val Leu Pro Tyr Phe Val Thr Pro 1 5 10 gat gct gct tac cac gga ttg aag tac gca gac ctt ggt gca cca gcc Asp Ala Ala Tyr His Gly Leu Lys Tyr Ala Asp Leu Gly Ala Pro Ala 25 ttc ggc ctc aag gtt cgc gtt ggc ctt cta cgc gac acc ggc tcc acc Phe Gly Leu Lys Val Arg Val Gly Leu Leu Arg Asp Thr Gly Ser Thr 40 ctc tcc gca ttc aac gca tgg gct gca gtc cag ggc atc gac acc ctt WO 01/00843 Leu Ser Ala Phe Asn Ala Trp Ala Ala Val Gln Gly Ile Asp PCT/IBOO/00923 ctg Leu ctc Leu gat Asp ggc Gly gca Ala 130 gat Asp tcc Ser cgc Arg cgc Arg aac Asn tcc Ser tc C Ser 115 ttt Phe gtt Val1 gac Asp ctg Leu ctg Leu aac Asri cc t Pro 100 gtt Val1 atc I le cgc Arg gaa Glu tcc Ser 180 gag Glu cac His tgg Trp ctc Leu gac Asp tcc Ser gct Ala 165 gtt Val1 cgC Arg gag Glu tac Tyr acc Thr gcc Ala ctc Leu 150 ggc G ly 9gc Gly cac His aag Lys gca Ala ttc Phe ctg Leu 135 gtt Val ctg Leu atc Ile aac gaa aac gcc Asn Glu Asn Ala 75 gtg gaa aag gtt Val Glu Lys Val 90 acc aag gaa aag Thr Lys Glu Lys 105 gag atc aag ggc Glu Ile Lys Gly 120 aag cta cac tcc Lys Leu His Ser gtt cac cca gca Val His Pro Ala 155 gca cgc gcg ggc Ala Arg Ala Gly 170 gag acc att gat Glu Thr Ile Asp 185 atc Ile aac Asn ctt Leu ggC Gly aac Asn 140 ac c Thr gtt Val ga t aag Lys t tc Phe ggC Gly aag Lys 125 Ct t Leu acc Thr acc Thr atc Thr gca Ala ggc Gly aag Lys gag Glu aac Asn cat His tcc Ser 175 gc t Leu gaa Glu c tg Leu tac Tyr gc t Ala atc Ile tca Ser 160 acc Thr gac Asp Ile Ile Ala Asp 190 ctc gaa ggc Leu Glu Gly 195 ggc ttt gct gca Gly Phe Ala Ala tagctttaaa tagactcacc cca <210> 248 <211> 200 <212> PRT <213> Corynebacterium glutamicum <400> 248 Thr Asp Glu Lys Asp Gly Lys Pro Val 1 5 Asp Ala Ala Tyr His Gly Leu Lys Tyr 25 Phe Gly Leu Lys Val Arg Val Gly Leu 40 Leu Ser Ala Phe Asn Ala Trp Ala Ala 55 Ser Leu Arg Leu Glu Arg His Asn Glu Leu 10 Ala Leu Val Asn Tyr Phe Val Thr Leu Gly Ala Pro Asp Thr Gly Ser Gly Ilie Asp Thr Ile Lys Val Ala WO 01/00843 Phe Leu As Lys Asp Se Thr Gly Se 11 Trp Ala Ph 130 Gly Asp Va 145 Gin Ser As Val Arg Le Leu Glu Gi Asn Pro 100 Val1 Ile Arg Giu Ser 180 Gly His Trp Leu Asp Ser Ala 165 Val Phe <210> 249 <211> 599 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (576) <223> FRXA00402 <400> 249 gta ttg ccc tac ttc gtc act cca Val Leu Pro Tyr Phe Val Thr Pro I 1 5 tac gca gac ctt ggt gca cca gcc I Tyr Ala Asp Leu Gly Ala Pro Ala 1 ctt cta cgc gac acc ggc tcc acc Leu Leu Arg Asp Thr Gly Ser Thr 1 40 gca gtc cag ggc atc gac acc ctt I Ala Val Gin Gly Ile Asp Thr Leu 55 gaa aac gcc atc aag gtt gca gaa I Glu Asn Ala Ile Lys Val Ala Glu 1 70 gaa aag gtt aac ttc gca ggc ctg Glu Lys Val Asn Phe Ala Gly Leu 1 aag gaa aag ctt ggc ctg aag tac Val1 Lys Gly Ser Ala 155 Gly Asp gc t Ala ctc Leu gca Ala cgc Arg aac Asn 75 tcc Ser tcc Asn Leu Gly Asn 140 Thr Val Asp tac Tyr aag Lys t tc Phe c tg Leu aac Asn cct Pro gtt Phe Gly Lys 125 Leu Thr Thr Ile cac His gtt Val1 aac Asn gag Giu cac His tgg Trp c tc PCTIBOOOO923 Gly Leu Lys Tyr Glu Ala Asn Ile His Ser 160 Ser Thr 175 Ala Asp gga ttg Gly Leu cgc gtt Arg Val gca tgg Ala Trp cgc cac Arg His aag Lys ggc Gly gc t Ala aac Asn gtg Val acc Thr gag WO 01/00843 Lys Giu Lys Leu Gly Leu Lys Tyr Thr 100 105 atc aag ggc ggc aag gat gag gct tgg Ile Lys Gly Gly Lys Asp Giu Ala Trp 115 120 cta cac tcc aac ctt gca aac atc ggc Leu His Ser Asn Leu Ala Asn Ile Gly 130 135 cac cca gca acc acc acc cat tca cag His Pro Ala Thr Thr Thr His Ser Gin 145 150 cgc gcg ggc gtt acc cag tcc acc gtc Arg Ala Giy Val Thr Gin Ser Thr Val 165 acc att gat gat atc atc gct gac ctc Thr Ilie Asp Asp Ile Ile Ala Asp Leu 180 185 tagctttaaa tagactcacc cca <210> 250 <211> 192 <2i2> PRT <2i3> Corynebacteriun glutamnicun <400> 250 Val Leu Pro Tyr Phe Vai Thr Pro Asp Ser ttt Phe gtt Val1 gac Asp 155 c tg Leu ggc Giy Val atc Ile cgc Arg 140 gaa Glu tcc Ser ggc Giy Leu gac Asp 125 tcc S er gc t Ala gtt Vai ttt Phe Thr 110 gcc Ala c tc Leu ggc Giy ggC Gly gc t Ala 190 PCTIBOOIOO923 Phe Glu ctg aag 384 Leu Lys gtt gtt 432 Val Val ctg gca 480 Leu Ala 160 atc gag 528 Ile Giu 175 gca atc 576 Ala Ile 1 Tyr Leu Al a Giu Giu Lys Ilie Leu His 145 Ala Leu Val Asn Lys Giu Lys His 130 Pro Asp Arg Gin Ala Val Lys Gly 115 Ser Ala Ala Thr Leu Giu Leu Tyr Ala 120 Ile Ser Phe Leu Ser Phe Lys Thr 105 Trp, Gly Gin Ala Gly Ser Leu Leu Asp Gly Ala Asp Ser Ala Leu Ala Arg Asn Ser Ser Phe Val1 Asp Tyr Lys Phe Leu Asn Pro Val1 Ile Arg 140 C iu WO 01/00843 WO 0100843PCTIBOO/00923 Arg Ala Gly Val Thr Gin Ser Thr Val Arg 165 170 Leu Ser Val Gly Ile Giu 175 Thr Ile Asp ASP Ile Ile Ala Asp Leu 180 185 Giu Gly Giy Phe Ala Ala Ile 190 <210> 251 <211> 613 <212> DNA <213> Corynebacterium <220> <221> CDS <222> (101)..(613) <223> RXS00405 <400> 251 agaataaatt tataccacac giutamicum agtctattgc aatagaccaa gctgttcagt agggtgcatg ggagaagaat ttcctaataa aaactcttaa ggacctccaa atg cca aag tac gac Met Pro Lys Tyr Asp aat tcc aat gct Asn Ser Asn Ala cag tgg ggc ttt gaa acc cgc tcc att Gin Trp, Gly Phe Giu Thr Arg Ser Ile cac gca His Ala ggc cag tca Gly Gin Ser gac gca cag acc agc gca cga aac ctt Asp Ala Gin Thr Ser Ala Arg Asn Leu ccg atc tac Pro Ile Tyr aag cag cgt Lys Gin Arg caa tcc acc gct ttc gtg ttc gac tcc gct gag cac Gin Ser Thr Ala Phe Val Phe Asp Ser Ala Giu His ttc gca Phe Ala ctt gag gat cta Leu Giu Asp Leu ggc Gly cct gtt tac tcc Pro Val Tyr Ser ctc acc aac cca Leu Thr Asn Pro ac c Thr gtt gag gct ttg Val Giu Ala Leu aac cgc atc gct tcc ctc gaa ggt ggc Asn Arg Ilie Ala Ser Leu Giu Gly Gly cac gct gta gcg His Ala Val Ala tcc tcc gga cag gcc gca acc acc aac Ser Ser Gly Gin Ala Ala Thr Thr Asn gcc att Ala Ile 100 ttg aac ctg Leu Asn Leu tac ggt ggc Tyr Gly Gly 120 gga gcg ggc gac Gly Ala Gly Asp atc gtc acc tcc Ile Val Thr Ser cca cgc ctc Pro Arg Leu 115 acc gag act cta Thr Glu Thr Leu t tc Phe 125 ctt atc act ctt aac cgc ctg ggt Leu Ile Thr Leu Asn Arg Leu Gly 130 atc gat gtt tcc ttc gtg gaa aac ccc gac gac cct gag tcc tgg cag WO 01/00843 Ile Asp Val 135 gca gcc gtt Ala Ala Val 150 aac cca cag Asn Pro Gin PCTII1BOO/00923 Giu Asn Pro Asp Asp Pro Glu Ser Trp Gin 140 145 acc aaa gca ttc ttc ggc gag act ttc gcc 595 Thr Lys Ala Phe Phe Gly Giu Thr Phe Ala 160 165 613 <210> 252 <211> 171 <212> PRT <213> Corynebacteriun giutamicun <400> 252 Met Pro Lys Tyr ASP Asn Ser Asn Ala Arg Asn His Arg Leu Thr Thr Leu Pro 145 G ly Gly Gin Phe Thr 70 His Leu Tyr Ile Ala 150 Asn Gln Ser Ala Val1 Ala Asn Gly Asp 135 Ala Pro Ser Thr Leu Glu Val1 Leu Gly 120 Val1 Val1 Gin Val Ala Glu Al a Ala Ala 105 Thr Ser Gin Al a Asp Asp Phe Asp Leu Phe Gly Giu Phe Pro Asp 170 Gin Ala Val Leu Glu 75 Ser Ala Thr Val Asn 155 Val1 Gly Thr Asp Pro Arg Gly Asp Phe 125 Asn Lys Phe Ser Ser Val1 I le Gin His 110 Leu Pro Ala Giu Ala Ala Tyr Ala Ala Ile Ile Asp Phe Thr Arg Giu Ser Ser Ala Val1 Thr Asp Phe 160 <210> 253 <211> 1812 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1789) <223> RXCO0164 WO 01/00843 WO 0100843PCTIiBOO/00923 <400> 253 ctgctttgcg ggaggttatg aaatgagtgg ggagacgtcg ggccagcctg ccgcaagtgc ggcgcgaggt ggcccggcag aaaagcatgc gctttccgtt cgg Arg gct Ala c tg Leu att Ile t tc Phe gaa Glu gat Asp gag Giu c tg Leu c aa Gin 150 tcc Ser gcg Ala cgt Arg aag Lys gcg Al a tcc Ser att Ile gtg Val1 atg Met ggc Gly tcc Ser 120 acc Thr gtg Val cac His atg Met act Thr 200 tgg Trp tcg Ser gat Asp gcg Ala gtg Val gtg Val1 tct Ser 105 gca Ala att I le ctc Leu tat Tyr gcg Ala 185 gtg Val1 tgg Trp gtg Val1 Giy gtg Val1 tcg Ser ggc Gly Gly gcg Ala gcc Ala att Ile t tg Leu 170 gag Glu cgg Arg ttt Phe c tg Leu gcg Ala gca Ala c9g Arg 75 acc Thr ga t Asp gtg Val gcg Ala cct Pro 155 agc Ser cgt Arg gcg Ala tta Leu gtg Vai cag Gin att Ile att Ile gcg Ala ttg Leu aca Thr acg Thr 140 gtc Val1 aag Lys gcg Ala tat Tyr ggC Gly ccg Pro atg Met 45 gcc Ala tc t Ser ctt Leu gtg Val1 gag Glu 125 atc Ile gtg Val gcg Ala cga Arg tcc Ser 205 ctg Leu 15 gtg Val1 gat Asp gcg Al a aag Lys ttg Leu 95 cgc Arg gtc Val1 gcg Ala gcg Al a gat Asp 175 gta Val1 gaa Glu gtg Val ctg Leu ttt Phe gtg Val1 att Ile 80 ccc Pro tcC Ser ccg Pro ctg Leu ccg Pro 160 c9g Arg ctt Leu gat Asp gtg ggt Val Gly 1 ttg ctg Leu Leu ggg cgg Gly Arg gtt gag Val Giu ctc agt Leu Ser atc gcc Ile Ala acg cac Thr His acc gat Thr Asp att tta Ile Leu 130 ttt tct Phe Ser 145 gtg tac Val Tyr tat gcg Tyr Ala gag gct Glu Ala gcc atg Ala Met 210 agt Ser att Ile 35 C tc Leu gcg Ala aat Asn cag Gin gat Asp 115 agt Ser ttg Leu tac Tyr gca Ala att Ile 195 cat His gcg Ala 20 gtg Val ag t Ser tgc Cys t tg Leu g tg Val 100 gtc Val tcc Ser gac Asp ttc Phe gaa Glu 180 cgc Arg aa t Asn ggc Gly ga t Asp gtg Val1 ggg Gly agg Arg gaa Glu tcc Ser tca Ser tgg Trp gcg Ala 165 cgc Arg ggg Gly cag Gin cgt att ccg Arg Ile Pro 115 163 211 259 307 355 403 451 499 547 595 643 691 739 787 att gat cag gcg tcg tgg tct gtg gtg gtc aag ggt att cgt gcg cgc WO 01/00843 Ile Asp Gin 215 acc acc atg Thr Thr Met 230 atg ctc gcg Met Leu Ala gcg ctg acg Aia Leu Thr ctg-cgt ggc Leu Arg Gly 280 tcc ggc tat 1027 Ser Giy Tyr 295 ccg att cct 1075 Pro Ile Pro 310 gaa ttg cgc 1123 Giu Leu Arg gac atc gac 1171 Asp Ile Asp gca tct ggc 1219 Aia Ser Giy 360 cgg gtg cca 1267 Arg Val Pro 375 cac ctc tct 1315 His Leu Ser 390 gag gtt cat 1363 Giu Vai His aaa cca gat 1411 Aia t tg Leu gtc Vali atc Ile 265 ccg Pro gcg Aia gtg Vali aac Asn atc Ile 345 gca Aia ga t Asp gac Asp gtt Vali gcc Ser at t Ile gcg Aila 250 ggc Giy atg Met tcg Ser ccc Pro gtc Vali 330 acg Thr ggt Giy caa Gin cgC Arg t tC Phe 410 tcc Val atg Met tac Tyr 255 gcc Ala cgc Arg gtg Val aaa Lys ggc Gly 335 gaa Giu gcc Aia gtc Vai cgc Arg cgc Arg 415 gcg Giy 225 ttt Phe c tg Leu ctg Leu C tc Leu gtt Val1 305 Cc t Pro tCC Ser gtc Val1 ttg Leu gac Asp 385 gcc Ala gat Asp gc t Ile gcg Aia gtc Vali atg Met gac Asp 290 gtt Val1 cag Gin t gg Trp gcg Aia C tg Leu 370 t tc Phe atg Met C tC Leu Ct t PCTIIBOO/00923 Ala Arg ttc ctc 835 Phe Leu 245 gat aat 883 Asp Asn 260 att cgt 931 Ile Arg att caa 979 Ile Gin gat ccg Asp Pro aaa gtg Lys Val 325 gtg aaa Val Lys 340 gtg ggc Vai Gly ggc ttg Gly Leu gtc tct Vai Ser agc cag Ser Gin 405 ttg gct Leu Aia 420 caa gtt WO 01/00843 Lys Pro Asp PCTIBOOOO923 aat gcc 1459 Asn Ala gtt ggt 1507 Val Gly 455 gcg ttg 1555 Ala Leu 470 gaa gcc 1603 Giu Ala gct gca 1651 Ala Ala cgg ttg 1699 Arg Leu ggg gag 1747 Gly Glu 535 Ala 425 gac Asp cga Arg Ser Asp Giu Glu tgg ttg gag agt Trp Leu Glu Ser 445 gga atc cag cta Gly Ile Gln Leu Ala His Ala Leu ctt cca gaa gga ctg gac acg gtc Leu Pro Glu Gly Leu Asp Thr Val 450 gaa cca gtg gtg gct cag cag ttg Glu Pro Val Val Ala Gln Gln Leu Gly Gin Val 435 460 gcc cgg gtg ttg Ala Arg Val Leu 475 acg gca gaa gca Thr Ala Glu Ala ttg ctc aat ccg gcg Leu Leu Asn Pro Ala 480 gga tcg gcg ggt gcc Gly Ser Ala Gly Ala 495 465 atc Ile gtc atc atg gat Val Ile Met Asp 485 agc gca ctg gaa gag Ser Ala Leu Giu Giu 500 gat gca Asp Ala 505 gat cag Aso~ Gin 490 gtg agc Val Ser aag aac cgt Lys Asn Arg 510 gca tcg cgg gct gat Ala Ser Arg Ala Asp tcc gca ttg gtg gtg gcg cac Ser Ala Leu Val Val Ala His 515 cag att ctg gtg atg gat aag Gin Ile Leu Val Met Asp Lys 530 cag gag tta ttg gat cac ggg Gin Giu Leu Leu Asp His Gly gtg gaa tcc ggt Val Glu Ser Gly 540 act cac Thr His ggt att tat cag cgt ctg tgg 1789 Gly Ilie Tyr Gin Arg Leu Trp 550 555 545 act gcg tgg agt gtc gga aga.

Thr Ala Trp Ser Val Gly Arg 560 tagttgactg ttcaatgcgt tga 1812 <210> 254 <211> 563 <212> PRT <213> Corynebacteriun giutamicun <400> 254 Val Gly Arg Ilie Pro Arg Ala Lys Trp Trp Phe Leu Gly Ala Leu Val 1 5 10 Leu Leu Ser Ala Gly Ala Tyr Ala Ser Val Leu Val Pro Gin Val Leu 25 Gly Arg Ilie Val Asp Leu Val Ser Asp Gly Ala Gin Met Arg Asp Phe 40 WO 01/00843 WO 0100843PCT/IBOO/00923 Val Glu Leu Ser Val Ile Leu Ile Ala Val Ala Ile Ala Gly Ala Val 55 Leu Ile Thr Thr Ile Phe 145 Val Tyr Giu Ala Gly 225 Phe Leu Leu Leu Val1 305 Pro Ser Val Ser Ala His Asp Leu 130 Ser Tyr Ala Ala Met 210 Ile Ala Val1 Met Asp 290 Val Gin Trp Ala Ala Cys Asn Leu Gin Val 100 Asp Val 115 Ser Ser Leu Asp Tyr Phe Ala Giu 180 Ile Arg 195 His Asn Arg Ala Giu Phe Ile Asp 260 Ilie Ile 275 Thr Ilie Ala Asp Giy Lys Ala Val 340 Leu Vai 355 Gly Arg Glu Ser Ser Trp Ala 165 Arg Gly Gin Arg Leu 245 Asn Arg Gin Pro Val1 325 Lys Gly Ser Gly Gly Ala Ala Ile Leu 170 Giu Arg Ser Ile Ala 250 Giy Met Ser Pro Val1 330 Thr Gly Arg Thr Asp Val Ala Pro 155 Ser Arg Ala Trp Leu 235 Leu Ala Asn Leu Asp 315 Ser Ile Lys Ile Ala Leu Thr Thr 140 Val Lys Ala Tyr Ser 220 Asn Val1 Val Met Ala 300 Ser Phe Asn Thr Ser Leu Val Giu 125 Ile Vai Ala Arg Ser 205 Val Met Ile Thr Phe 285 Arg Gly Ser Ser Thr 365 WO 01/00843 Leu Leu Ala 370 Asp Phe Pro 385 Ala Met Val Asp Leu Thr Ala Leu Gly 435 Gly Leu Asp 450 Val Ala Gin 465 Ile Val Ilie Ser Ala Leu Leu Val Val 515 Leu Val Met 530 Leu Leu Asp 545 Val Gly Arg Gly Val1 Ser Leu 420 Gin Thr Gin Met Giu 500 Ala Asp His Leu Ser Gin 405 Ala Val1 Val1 Leu Asp 485 Giu His Lys Gly Arg His 390 Glu Lys Asn Val Ala 470 Glu Al a Arg Gly Gly 550 Val1 375 Leu Val Pro Ala Gly 455 Leu Ala Ala Leu Glu 535 Ile Pro Ser His Asp Leu 440 Ala Ala Thr Asp Asp 520 Val1 Tyr

ASP

Asp Val1 Ala 425 Asp Arg Arg Ala Ala 505 Gin Val Gin Gly Giu 395 Ser Asp Leu Ile Leu 475 Ala Ser Ser Ser Leu 555 Gin 380 Arg Gly Glu Giu Gin 460 Leu Gly Lys Arg Gly 540 Trp Val1 Ile Thr Giu Ser 445 Leu Leu Ser Asn Ala 525 Thr Thr Leu Ala Leu Leu 430 Leu Glu Asn Ala Arg 510 Asp His Ala PCTIiBOO/00923 Val Asp Arg Leu 400 Arg Gin 415 Ala His Pro Giu Pro Vai Pro Ala 480 Gly Ala 495 Ser Ala Gin Ile Gin Giu Trp Ser 560 <210> 255 <211> 1713 <212> DNA <213> Corynebacterium giutanicun <220> <221> CDS <222> (101)..(1690) <223> RXCO1191 <400> 255 cgctgctttc acgcaactga aaccgcaccg gatcaagtta tttggggttg ttctttgtgg cgtgttggtg gccgtcgcgg ggttggtagg gccctgggcg gtg ggt gga ctc gtc 115 Val Gly Giy Leu Val 1 gat aag ctc ctt gca acc ccg agc atg cgc gac gtt gta gtg ttc gcg 163 Asp Lys Leu Leu Ala Thr Pro Ser Met Arg Asp Val Val Val Phe Ala 15 WO 01/00843 ctg Leu ggC Gly cgc Arg gaa Giu cgg Arg gcg Ala tgg Trp acg Thr cgc Arg 150 ggC Gly cgt Arg ttc Phe atc Ile gat Asp 230 cga Arg atc gtg gct Ile Val Ala gcg ctg atg Ala Leu Met gat gtg ttg Asp Val Leu gcg ggg cgc Ala Gly Arg gtg tcc act Val Ser Thr ttt acc gtg Phe Thr Val 105 ctc ggc ctt Leu Gly Leu 120 cgc gtc tat Arg Val Tyr gcc ttt ggg Ala Phe Gly gaa acc ttg Glu Thr Leu 170 gac gca gcc Asp Ala Ala 185 ttc ctc aca Phe Leu Thr 200 ctc gtg ctc Leu Val Leu gtc acc gtc Val Thr Val ttc ggt cca Phe Gly Pro 250 ggc ggc gtt gtt tcg agc ctg ggc Gly Gly Val Val Ser Ser Leu Gly 30 gcg Ala cgc Arg ggc Gly 75 gcg Ala gtg Val1 gtc Val tta Leu gtg Val 155 cgc: Arg tcC Ser tgg Trp atc Ile ggc Gly 235 atc Ile cgc Arg gcg Ala gac Asp gcg Ala att Ile ggt Gly ccc Pro 140 cgc Arg gc t Ala ggc Gly gca Ala t tg Leu 220 gca Ala ggc Gly atc 1.1e gcg Ala gcg Ala c tg Leu gcg Al a ctg Leu 130 tat Tyr ggc Gly aca Thr tcc Ser cgc Arg 210 ctg Leu ctg Leu ttc Phe PCTIIBOO/00923 acg tgg tgg 211 Thr Trp, Trp gcg ggg ctg 259 Ala Gly Leu aac acg att 307 Asn Thr Ile gat gat tcg 355 Asp Asp Ser atg gtg cag 403 Met Val Gin 100 gcg gtt gat 451 Ala Val Asp 115 tat tgg acc 499 Tyr Trp Thr acg cgt gag 547 Thr Arg Glu gca gtc gaa 595 Ala Val Glu 165 gaa tta aag 643 Glu Leu Lys 180 att tct gtt 691 Ile Ser Val 195 gcg gaa tgc 739 Ala Glu Cys gtc aac atc 787 Val Asn Ile atc ttc cac 835 Ile Phe His 245 tcc gac atc 883 Ser Asp Ile 260 caa tcc gcc agc gca tcg ctg atc cgc atg gtg ggc gtt att aac gcg WO 01/00843 Gin Ser Ala gca tcg aac Ala Ser Asn 280 PCTIIBOO/00923 Ser 265 cag Gin Ala Ser Leu Ile met Val Gly Val Ile Asn Ala 275 agc acc gct Ser Thr Ala gtc agc ggc Val Ser Gly ccg gcg tct Pro Ala Ser tta acg 1027 Leu Thr 295 aag aat 1075 LYS Asn 310 ggt gcg 1123 Giy Ala ctg ctc 1171 Leu Leu tct aac 1219 Ser Asn caa gaa 1267 Gin Giu 375 gca cgc 1315 Ala Arg 390 att ggt 1363 Ilie Gly gtg ggt 1411 Val Gly gcg ctt 1459 Ala Leu gaa gca 1507 Glu Ala 455 ctt ttc gac gtc tcc Leu Phe Asp Val Ser 300 gca tcc gtg cag ctg Ala Ser Val Gin Leu 315 acc ggc gct ggt aaa Thr Gly Ala Gly Lys 330 agc cca act tcc ggg Ser Pro Thr Ser Gly cac tat cac act gca ccc gtc atc His Tyr His Thr Ala Pro Val Ile 305 cca ggg gaa cac atc gcc att gtg Pro Glv Giu His Ile Ala Ile Val gaa 320 agc acg ctc Ser Thr Leu 335 gcc Ala ctc att gcg gca ggc Leu Ile Ala Ala Gly 340 cag gtg Gin Val gct ctc ggc gga tcg agt ttt Ala Leu Gly Giy Ser Ser Phe 355 345 gtc gaa Val Giu 350 ccg gaa gca ttg cgc Pro Giu Ala Leu Arg 365 tgc ttc cga gga tct Cys Phe Arg Gly Ser cag Gin gt t Val1 cac His 380 ccc gaa gcc acc Pro Giu Ala Thr 395 gat tcc tgg ttg Asp Ser Trp Leu gat gcg gac atc ASP Ala ASP Ile gag cgc tta ccg Giu Arg Leu Pro aag atc gcg atg gtc agc Lys Ile Ala Met Val Ser 370 tta gat aat ctt cgt atc Leu Asp Asn Leu Arg Ile 385 cac gcc gtt ctc gcc gat His Ala Val Leu Ala Asp 400 405 caa ggc ata gac acc atc Gin Giy Ile ASP Thr Ilie 420 gtg gaa aac cag atc atg Val Giu Asn Gin Ile Met 435 gca atc gtc atc ctt gat Aia Ile Val Ile Leu Asp gat ggc Asp Gly 410 415 gct ttc cgt tta acc tct Ala Phe Arg Leu Thr Ser 430 gta cat ttg gcc gac cta Val His Leu Ala Asp Leu gct Ala 440 acg Thr gct gaa tca ggc Ala Giu Ser Gly 460 445 tct Ser gat cat gca aaa Asp His Ala Lys 465 450 cag ctt gaa gat Gin Leu Giu Asp WO 01/00843 WO 0100843PCT/IBOO/00923 gca gcc ctt aaa gtc act gaa aac aga 1555 Ala Ala Leu Lys Val Thr Glu Asn Arg 470 475 cgc ctc aac caa gcg aaa acc gcc gat 1603 Arg Leu Asn Gin Ala Lys Thr Ala Asp 490 gga gaa atc ata gaa tct gga acc cat 1651 Gly Giu Ile Ilie Glu Ser Gly Thr His 505 510 ggc cga tat gaa caa ctg tgg act gcg 1700 Gly Arg Tyr Glu Gin Leu Trp Thr Ala 520 525 cccaagacca cgc 1713 <210> 256 <211> 530 <212> PRT <213> Corynebacteriun glutamTicum <400> 256 Val Gly Gly Leu Val Asp Lys Leu Leu gcc Ala 480 atc Ile gag Giu tc t Ser Thr Gly Ala Arg Gly Ala Val Val1 Leu Val 155 atc Ile atc Ile ctt Leu gcg Ala Pro Gly Arg Ala Asp Ala Ile Gly Pro 140 Arg atc gtg gct cac Ile Val Ala His 485 gtc atg gac tcc Val Met Asp Ser 500 cga gcg atc ggc Arg Ala Ile Gly 515 cgc taattagcca Arg 530 Val1 Gly Ile Ala Ala Leu Ala Leu 130 Tyr Val1 Thr Ala Asn Asp Met Ala 115 Tyr Thr Phe Trp Gly Thr Asp Val1 100 Val1 Trp Arg met Val1 Leu Val1 Ile Thr Ala 110 Val Ser Gin WO 01/00843 Val Gly Ala Asp Thr Glu Ile Ser Ile 195 Asn Arg Ala 210 Tyr Leu Val 225 Ala Leu Ilie Met Phe Ser Giy Val Ile 275 Ser Ala Ser 290 Thr Ala Pro 305 Hius Ile Ala Leu Ilie Ala Gly Gly Ser 355 Ilie Ala Met 370 Asp Asn Leu 385 Ala Val Leu Gly Ile Asp Giu Asn Gin 435 Ile Val Ile 450 Lys Gin Leu 465 Giu 165 Lys Vai Cys Ilie His 245 Ile Al a Ala Ilie Val1 325 Gly Phe Ser Ile Asp 405 Ile Met Asp Asp Gly Arg Phe Ile Asp 230 Arg Gin Ala Leu Lys 310 Gly Leu Ser Gin Ala 390 Ile Val1 Ala Giu Ala 470 Ala Ile Arg Thr 215 Leu Leu Ser Ser Thr 295 Asn Aia Leu Asn Giu 375 Arg Gly Gly Leu Ala 455 Ala Giu Asp Phe 200 Leu Val Phe Ala Asn 280 Leu Ala Thr Ser Vai 360 Ile Pro Asp Asp Ala 440 Thr Leu Thr Aia 185 Leu Val Thr Giy Ser 265 Gin Phe Ser Gly Pro 345 Giu His Giu Ser Gly 425 Arg Ala Lys PCTIBOOIOO923 Ala Giu 175 Arg Asp Arg Asn Gly Phe Thr Ala 240 Val Gly 255 Met Val Pro Ala Tyr His Gly Giu 320 Leu Aia 335 Ala Leu Gin Lys Val Leu Ile His 400 Pro Gin 415 Ser Val Leu Ala His Ala Ser Ala 480 Ile Ilie Val Ala His Arg Leu Asn Gin Ala Lys Thr Ala Asp Arg Ile WO 01/00843 WO 0100843PCT/IBOO/00923 485 Ile Vai Met Asp Ser Giy Giu Ile Ile 500 505 Leu Arg Ala Ilie Gly Giy Arg Tyr Giu 515 520 Giu Ser Giy Thr His Giu Giu 510 Gin Leu Trp Thr Ala Trp Ser Ala Arg 530 <210> <211> <212> <213> <220> 257 1392

DNA

Corynebac terium glutainicum <221> CDS <222> (101)..(1369) <223> RXA02646 <400> 257 ttggaaataa aacctatgcc aaagtaggtg caattctagg agaagattac actagtcaac catgagtgaa acatacgtgt ctgagaaaag tccaggagtg atg gct agc gga gcg Met Ala Ser Gly Ala gag ctg att cgt Giu Leu Ile Arg gcc gac att caa Ala Asp Ilie Gin gcg cag gca cga Ala Gin Ala Arg att tcc Ile Ser tcc gtc att Ser Val Ilie gaa acc gga Giu Thr Gly cca act cca ttg Pro Thr Pro Leu tat tgc cct cgt Tyr Cys Pro Arg ctt tct gag Leu Ser Giu cag gat gtt Gin Asp Val 211 259 gcg gaa atc tac Ala Giu Ile Tyr aag cgt gag gat Lys Arg Giu Asp cgt tcc Arg Ser tac aag atc cgc Tyr Lys Ilie Arg gcg ctg aac tct Ala Leu Asn Ser gcg cag ctc acc Ala Gin Leu Thr caa Gin gag cag cgc gat Giu Gin Arg Asp gca Al a 75 ggt atc gtt gcc gca tct gca ggt aac Gly Ile Val Ala Ala Ser Ala Gly Asn 80 cat His gcc cag ggc gtg Ala Gin Gly Val tat gtg tgc aag Tyr Val Cys Lys ttg ggc gtt cag Leu Gly Val Gin gga cgc Gly Arg 100 atc tat gtt Ile Tyr Val gtt cac ggc Val His Gly 120 gtg cag act cca aag caa aag cgt gac Val Gin Thr Pro Lys Gin Lys Arg Asp cgc atc atg Arg Ile Met 115 403 451 499 gga gag ttt gtc Gly Giu Phe Vai tcc Ser 125 ttg gtg gtc act ggc aat aac ttc Leu Val Val Thr Gly Asn Asn Phe 130 WO 01/00843 gac gaa gca Asp Glu Ala 135 acg ctg atc Thr Leu Ile 150 acc gtg gct Thr Val Ala gca gat cac Ala Asp His gtg gtc agc Val Val Ser 200 atc gaa cca Ile Glu Pro 215 gga cca atc Gly Pro Ile 230 gtc aaa cgt Val Lys Arg ggt cgc gtg Gly Arg Val atg ctc gat Met Leu Asp 280 gcg ctg tct 1027 Ala Leu Ser 295 gtc gtg gtg 1075 Val Val Val 310 gcg gaa atc 1123 Ala Glu Ile ttc ttg gtg 1171 Phe Leu Val tcg Ser gag Giu gct Ala gtg Val 185 tac Tyr gcg Ala act Thr gtc Val cac His 265 ctt Leu atc Ile tgc Cys gc t Ala aac Asn 345 gc t Al a cc t Pro gag Glu 170 atg Met atg Met gga Gly ttg Leu gga Gly 250 atg Met t ac Tyr gc t Ala atc Ile gag Glu 330 ttc gcg Ala 140 ga t Asp ttg Leu cca Pro gat Asp gca Ala 220 act Thr c tc Leu agc Ser aac Asn ttg Leu 300 tct Ser tcc Ser caa cat His gc t Ala tcg Ser gtc Val atg Met 205 tcc Ser gt t Val aac Asn gcg Ala gaa Glu 285 aag Lys ggt Gly t tg Leu aag gaa Glu cgc Arg cag Gin Gly 190 gca Ala atg Met gat Asp tac Tyr acc Thr 270 9gc Gly gaa Glu ggc Gly gtg Val1 cc t Pro 350 ga t Asp aac Asn ctg Leu 175 ggt Gly cc t Pro cag Gin ccc Pro acc Thr 255 gag Giu atc Ile atg Met aac Asn cgc Arg 335 ggt Gly gc a Ala ac c Thr 160 act Thr 9gc Gly dgc Arg gc t Ala tt t Phe 240 atc Ile Gly atc Ile t cc Ser aac Asn 320 cgc Arg cag Gin gag Glu 145 gtc Val tcc Ser gga G ly act Thr gca Ala 225 gt g Val1 gtg Val1 gc t Ala gcg Ala ttt Phe 305 ga t Asp ggt G ly ttg Leu cgc Arg atc Ile atg Met ctt Leu gcg Ala 210 ttg Leu gac Asp gag Glu gtg Val gag Glu 290 gca Ala gtg Val1 tta Leu cgt Arg acc Thr ggt Gly ggc Gly ctt Leu 195 atc Ile cac His ggc Gly aag Lys tgt Cys 275 cc t Pro gc t Ala ctg Leu aag Lys cac His 355 PCT/1BO0100923 ggc gca 547 Gly Ala cag ggc 595 Gin Gly 165 aag agt 643 Lys Ser 180 gca ggt 691 Ala Gly gtt ggt 739 Val Gly aat ggt 787 Asn Gly gca gca 835 Ala Ala 245 aac cag 883 Asn Gin 260 act gag 931 Thr Glu gct ggc 979 Ala Gly cgc tct Arg Ser cgt tat Arg Tyr 325 cac tac His Tyr 340 ttc ctg Phe Leu Phe Pro Gin Lys WO 01/00843 gaa gat atc ctg gga ccg gat gat gac 1219 Glu Asp Ile Leu Gly Pro Asp Asp Asp 360 365 aag cgc aac aac cgt gag acc ggt act 1267 Lys Arg Asn Asn Arg Giu Thr Gly Thr 375 380 agt gaa gca tca gga ttg gat tct ttg 1315 Ser Giu Ala Ser Gly Leu Asp Ser Leu 390 395 gca att gat tcC cgt cgc ctc gag ccg 1363 Ala Ilie Asp Ser Arg Arg Leu Glu Pro 410 ttg acc taaacatagc tgaaggccac ctc 1392 Leu Thr <210> 258 <211> 423 <212> PRT <213> Corynebacteriui giutamicun <400> 258 Met Ala Ser Gly Ala Giu Leu Ilie Arg atc Ile gcg Ala Ctg Leu ggc Gly 415 gag Giu att Ile gag Glu tac Tyr PCTJIBOO/00923 tac ctc Tyr Leu cac ttg His Leu gaa tcg Glu Ser 405 gaa tac Glu Tyr 420 1 Gin Pro Asp Gly Ser Gly Arg Thr Glu 145 Ser Giu Arg Gin Ala Ile Val1 Asp Thr 150 Ile Gly Tyr Gin Giy Vali Giy 120 Ala Ile Ala Pro Giu Ilie Asp Ala Vali Giu Ala Pro WO 01/00843 WO 0100843PCTIiBOO/00923 Val S er G ly Thr Ala 225 Val1 Val Ala Ala Phe 305 Asp Gly Leu Leu Val1 385 Arg Pro Gly Gly Leu 195 Ile His Gly Lys Cys 275 Pro Ala Leu Lys His 355 Giu I le Gi u Tyr Gin Lys 180 Ala Val1 Asn Ala Asn 260 Thr Ala Arg Arg His 340 Phe Tyr His Glu Glu 420 Gly Thr Vai 165 Ser Ala Asp Gly Vai Val Giy Ile Glu 215 Gly Gly Pro 230 Ala Val Lys 245 Gin Gly Arg Giu Met Leu Giy Ala Leu 295 Ser Val Val 310 Tyr Ala Giu 325 Tyr Phe Leu Leu Giu Asp Leu Lys Arg 375 Leu Ser Glu 390 Ser Ala Ile 405 Tyr Leu Thr Ile Val1 Ala Ala Giu 235 Asp Met Gin Gly Ile 315 Arg Pro Pro Giu Leu 395 Leu Pro Asp Ala 220 Thr Leu S er Asn Leu 300 Ser Ser Gin Asp Thr 380 Asp Ser Val Met 205 Ser Val Asn Ala Giu 285 Lys Gly Leu Lys Asp 365 Gly Ser Gin G ly 190 Ala Met Asp Tyr Thr 270 G ly Giu Gly Val1 Pro 350 Asp Thr Leu Ser Arg Arg Leu Glu Pro Giy Thr 410 415 <210> 259 <211> 966 <212> DNA <213> Corynebacterium giutamicui <220> <221> CDS <222> (101)..(943) WO 01/00843 WO 0100843PCTIBOOIOO923 <223> RXA00766 <400> 259 tatggcgctt gaaccacaaa taaagtctgc accgacgccg gtgatcttaa ttgtcgaacc ctacggcggg tccatccggc aacaaaaccc caacctacca atg gtt ttt tgg Met Val Phe Trp gat gca gcc tta acc cga ggc gat ggc atc ttt gaa aca ctc Asp Ala Ala Leu Thr Arg Gly Asp Gly Ile Phe Glu Thr Leu ctc atc Leu Ile cgc gac gga cat gcc tgc aac gtg Arg Asp Gly His Ala Cys Asn Val gca tcg gca gca cta ttg gga ctg Ala Ser Ala Ala Leu Leu Gly Leu cga cac gga gaa Arg His Gly Giu cgc ttc aaa Arg Phe Lys gaa gac tgg Giu Asp Trp cca gag ccg atc Pro Giu Pro Ile gaa aaa Giu Lys gcc acc caa atg Ala Thr Gin Met ggc Gly atc gaa tcc tgg Ile Giu Ser Trp tcc cac ccc aac Ser His Pro Asn gca Ala ggc gag gcc tca Gly Giu Ala Ser acc tgg acg ctc agc cga ggt cgt tcc Thr Trp Thr Leu Ser Arg Gly Arg Ser acg ggg ctg gcc Thr Gly Leu Ala gga tgg tta acc Gly Trp Leu Thr acc cca gtc tcc Thr Pro Val Ser tcc gac Ser Asp 100 aaa ctg gcg Lys Leu Ala gga tat tcc Giy Tyr Ser 120 cgt gaa cac ggt Arg Giu His Gly gta Val 110 tcg gtc atg acc Ser Val Met Thr agt tca aga Ser Ser Arg 115 gcc acc cga Ala Thr Arg atc gac acc ggc Ilie Asp Thr Gly ctc Leu 125 ccc gga atc gga Pro Gly Ile Gly ggc gag Gly Giu 135 cta tct aaa gtg Leu Ser Lys Val cga acc ccc gca Arg Thr Pro Ala tgg ctg aca gtc Trp Leu Thr Val 9gc Gly 150 gcc Ala gcc aaa aca cta Ala Lys Thr Leu aaa tca aac gga Lys Ser Asn Gly 170 tac gca gca aac Tyr Ala Ala Asn atg Met 160 gca gcc ctg cgc Ala Ala Leu Arg tac Tyr 165 ttc gac gat gtg Phe Asp Asp Val atc Ile 175 ttc acc gat ggc Phe Thr Asp Gly gac cgc Asp Arg 180 gtt cta gaa Val Leu Giu atc cgc acc Ilie Arg Thr 200 gcc acc tcc acc Ala Thr Ser Thr gta Val 190 gtg agt ttc aaa Val Ser Phe Lys ggc gac aaa Gly ASP Lys 195 acc acc caa Thr Thr Gin cct tca ccc ggt Pro Ser Pro Gly ggC Gly 205 gac att ctc ccc Asp Ile Leu Pro gga Gly 210 WO 01/00843 gca gca ctc ttt Ala Ala Leu Phe 215 aaa gac tta agc Lys Asp Leu Ser 230 gtg tcc tcc gtc Val Ser Ser Vai gca cac gca acc gaa aaa gga Ala His Ala Thr Glu Lys Gly 220 att gac gat ctt ttc gga gcc Ile Asp Asp Leu Phe Gly Ala 235 240 cgc gga cca gtt cgg gtg acc Arg Gly Pro Val Arg Val Thr 250 255 cca gac aat gaa aaa gaa atc Pro Asp Asn Glu Lys Glu Ile 270 tagaggctgg cgctgggact tgc cga tgt Arg Cys agc gtg Ser Val ctc gat Leu Asp gcg ctg Ala Leu 275 PCT/iBOO/00923 aaa gaa 787 Lys Glu tgg cta 835 Trp Leu 245 gga cac 883 Gly His 260 att acc 931 Ile Thr aaa Lys aaa Lys tta Leu gc t Ala <210> 260 <211> 281 <212> PRT <213> Corynebacteriun giutamicun <400> 260 Met Val Phe Trp Asp Asp Ala Ala Leu 1 Glu Gly Ile Tyr Arg Pro Met Gly Pro 145 Ala Thr Leu Arg Giu His Arg Ser Ser 115 Ala Leu Leu Gly Leu Phe Asp Pro Ser Ser 100 Ser Thr Thr Arg Asp 5 Ile Lys Trp Asn Ser Asp Arg Arg Val1 Tyr 165 Arg Arg Ala Glu Ala 70 Thr Lys Gly Gly Giy 150 Ala Val1 Asp S er Lys Gly G ly Leu Tyr Glu 135 Ala Lys Leu Gly Ala Ala Glu Leu Ala Ser 120 Leu Lys Ser Glu Thr Arg 10 Ala, Cys Leu Leu Gin Met Ser Cys Ser Gly 90 Arg Giu Asp Thr Lys Val Leu Ala 155 Gly Phe 170 Ala Thr Ile Arg Glu Ser Leu Ile Ser Gly Pro Asn Ile 175 Val1 WO 01/00843 WO 0100843PCTIIBOO/00923 Phe Pro Trp 225 Asp Arg Lys Lys Gly 210 Arg Ser Leu Ala Gly 195 Thr Cys Val Asp Leu 275 180 Asp Thr Lys Trp Gly 260 Ile Arg Ala 215 Asp Ser Leu Ala Thr 200 Leu Leu Ser Arg Leu 280 185 Pro Phe Ser Val1 Lys 265 Gly Ser Pro Gly Gly 205 Ala His Ala Thr 220 Ile Asp Asp Leu 235 Arg Gly Pro Val 250 Pro Asp Asn Glu Ile Lys Gly Val 255 Glu <210> 261 <211> 1224 <212> DNA <213> Corynebacteriun glutamicum <22 0> <221> CDS <222> (101). .(1201) <223> RXN01690 <400> 261 cctagccatt cctcaaaacc gtgagacgaa attggctatt actagtgtat ctgtcaggta gcaggtgtac cttaaaatcc ttc Phe gaa Glu gtg Val1 gtg Val tac TIyr gaa Glu ac c Thr gcc Al a gac Asp gcg Ala gca Ala aag Lys acc Thr ccg Pro aac Asn att Ile ttt Phe 75 t tc Phe acg Thr aag Lys ggc Gly cc t Pro aag Lys gaa Glu catcccataa aatggggctg atg acg tca tta gag Met Thr Ser Leu Glu 11. ccc gat cgt ctg aag Pro Asp Arg Leu Lys ttc acc gac cac atg Phe Thr Asp His Met cac aac gcc caa tta His Asn Ala Gin Leu acc acc gta ttc cac Thr Thr Val Phe His tac cgc cat tcg gac Tyr Arg His Ser Asp gcc gag cgt atg cag Ala Glu Arg Met Gin 100 WO 01/00843 WO 0100843PCTIBOO/00923 cgt tca Arg Ser att aaa Ile Lys gag tac Glu Tyr 135 acc gaa Thr Giu 150 gtc atc Val Ile gtt tcC Val Ser act ggt Thr Gly cag tcc Gin Ser 215 gcc atc Ala Ile 230 ttc atc Phe Ile tcC ggc Ser Gly gca cgc Ala Arg gag tgg 1027 Glu Trp 295 tgC ggt 1075 Cys Gly 310 cac ggc 1123 cga Arg gaa Glu gaa Giu t tg Leu cca Pro 170 c tg Leu aaa Lys gcg Ala aag Lys aac Asn 250 ctt Leu gga Gly gac Asp gc t Al a gaa atg Met c tg Leu gc t Ala ggt Gly 155 gtc Val agc Ser ttt Phe gaa Glu tac Tyr 235 ggc Gly cca Pro tac Tyr gca Al a gtt Val 315 gtg gca Ala ctg Leu tcc Ser 140 gtc Val ggc Gly gaa Giu gc t Ala aag Lys 220 atc Ile gac Asp ggC Gly gaa Glu aag Lys 300 atc Ile aac atg Met gta Val1 125 ctc Leu agc Ser gc t Ala ga t Asp ggc Gly 205 ggc Gly gaa Glu caa Gin atc Ile gta Val 285 tct Ser acc Thr aac cca Pro 110 gac Asp tac Tyr cca Pro tac Tyr tac Tyr 190 aac Asn tgt Cys gaa Giu gtC Val1 acc Thr 270 gaa Glu ggc Giy cc t Pro aa t t tg Leu ga t Asp cgc Arg ga t Asp 160 acc Thr cgc Arg gcg Ala cag Gin ggt Gly 240 cta Leu aag Lys cga Arg a tg Met ggc Gly 320 gtC cca Pro cag Gin cca Pro 145 gcc Ala ggt Gly gc t Al a gct Ala gtc Val1 225 ggc Gly gtc Val tca Ser aag Lys ac c Thr 305 acc Thr gga acc Thr ga t Asp 130 ttC Phe tac Tyr gga Gly gca Ala tct Ser 210 gta Val atg Met acc Thr ctt Leu atc Ile 290 gag Giu gtg Val1 gaa gag Giu 115 tgg Trp atg Met aag Lys atc Ile ccc Pro 195 ttg Leu tgg Trp aac Asn cct Pro cta Leu 275 acc Thr gca Ala aaa Lys atc gac Asp gt t Val1 atc Ile t tc Phe aag Lys 180 ggc Gly ctt Leu ttg Leu ctt Leu gaa Giu 260 caa Gin acc Thr ttt Phe tca Ser acg 451 499 547 595 643 691 739 787 835 883 931 979 WO 01/00843 His Gly Thr Phe Glu Val Asn Asn Asn 330 aag ctt cgt gaa acc ctc acc gga att 1171 Lys Leu Arg Giu Thr Leu Thr Gly Ile 345 350 caa aac gga tgg ctt tac cca ctg gtt 1221 Gin Asn Gly Trp Leu Tyr Pro Leu Val 360 365 ccc 1224 <210> 262 <211> 367 <212> PRT <213> Corynebacterium giutamicun <400> 262 Met Thr Ser Leu Giu Phe Thr Vai Thr PCTIBOOIOO923 Giu Val Gly Giu Ile Thr Met 335 340 cag caa gga aac gtt gaa gac Gin Gin Gly Asn Val Glu Asp 355 ggc taaatcaacc ggttttaaga Giy 1 Pro Phe His Thr Tyr Aia Pro Gin Pro 145 Ala Gly Ala

ASP

Thr Asn Thr Arg Giu Thr

ASP

130 Phe Tyr Gly Ala Arg Asp Ala Val1 His Arg Giu 115 Trp Met Lys Ile Pro Leu His Gin Phe Ser Met 100 Asp Val Ile Phe Lys 180 Gly 5 Lys Met Leu His Asp Gin Phe Pro Ser Leu 165 Pro G iy Ile Thr Pro Gly Thr Ser Lys Tyr 135 Giu Ile Ser Gly Leu Ile 40 Tyr Gin Ile Al a Ala 120 Gly Ile Ala Val1 Asp 200 Asn Phe Ser Met Gly Pro Met Val1 125 Leu Ser Ala Asp Gly 205 Pro Gly Glu Asp Ile Asp Pro 110 Asp Tyr Pro Tyr Tyr 190 Asn Thr Lys Gly Pro Lys Giu Gin Ala Leu Ala Phe 175 Val Tyr WO 01/00843 WO 0100843PCTIBOO/00923 Ala Ser Leu Leu Ala Gin Ser Gin Ala Ala Glu Lys Gly Cys Asp Gin 210 215 220 Val Val Trp Leu Asp Ala Ile Glu His Lys Tyr Ile Glu Glu Met Gly 225 230 235 240 Gly Met Asn Leu Gly Phe Ilie Tyr Arg Asn Gly Asp Gin Val Lys Leu 245 250 255 Val Thr Pro Giu Leu Ser Gly Ser Leu Leu Pro Gly Ile Thr Arg Lys 260 265 270 Ser Leu Leu Gin Val Ala Arg Asp Leu Gly Tyr Giu Val Giu Giu Arg 275 280 285 Lys Ile Thr Thr Thr Giu Trp Giu Giu Asp Ala Lys Ser Gly Ala Met 290 295 300 Thr Giu Ala Phe Ala Cys Gly Thr Ala Ala Val Ile Thr Pro Val Gly 305 310 315 320 Thr Val Lys Ser Ala His Gly Thr Phe Giu Val Asn Asn Asn Giu Vai 325 330 335 Gly Giu Ilie Thr Met Lys Leu Arg Giu Thr Leu Thr Giy Ilie Gin Gin 340 345 350 Gly Asn Vai Giu Asp Gin Asn Gly Trp, Leu Tyr Pro Leu Val Gly 355 360 365 <210> 263 <211> 1076 <212> DNA <213> Corynebacteriun giutaxnicum <22 0> <221> CDS <222> (1053) <223> FRXA01690 <400> 263 ccc gat cgt ctg aag gaa att ctt gcc gca ccg aag ttc ggt aag ttc 48 Pro Asp Arg Leu Lys Giu Ile Leu Ala Ala Pro Lys Phe Gly Lys Phe 1 5 10 ttc acc gac cac atg gtg acc att gac tgg aac gag tcg gaa ggc tgg 96 Phe Thr Asp His Met Val Thr Ilie Asp Trp Asn Giu Ser Giu Gly Trp 25 cac aac gcc caa tta gtg cca tac gcg ccg att cct atg gat cct gcc 144 His Asn Ala Gin Leu Val Pro Tyr Ala Pro Ile Pro Met Asp Pro Ala 40 acc acc gta ttc cac tac gga cag gca att ttt gag gga att aag gcc 192 Thr Thr Val Phe His Tyr Gly Gin Ala Ile Phe Giu Gly Ilie Lys Ala 55 tac cgc cat tcg gac gaa acc atc aag act ttc cgt cct gat gaa aac 240 Tyr Arg His Ser Asp Glu Thr Ile Lys Thr Phe Arg Pro Asp Giu Asn 384 WO 01/00843 WO 0100843PCT/iBOO/00923 gcc gag cgt atg Ala Glu Arg Met cgt tca gca gct Arg Ser Ala Ala atg gca atg cca Met Ala Met Pro cag ttg Gln Leu cca acc gag Pro Thr Glu cag gat tgg Gin Asp Trp, 115 ttt att aaa gca Phe Ilie Lys Ala gaa ctg ctg gta Glu Leu Leu Val gac gcg gat Asp Ala Asp 110 tac ctg cgc Tyr Leu Arg gtt cct gag tac Val Pro Giu Tyr gga gaa gct tcc Gly Giu Ala Ser ctc Leu 125 cca ttc Pro Phe 130 atg atc tcc acc Met Ile Ser Thr att ggc ttg ggt Ile Gly Leu Gly gtc Val 140 agc cca gct gat Ser Pro Ala Asp tac aag ttc ctg Tyr Lys Phe Leu atc gca tcc cca Ile Ala Ser Pro ggc gct tac ttc Gly Ala Tyr Phe ggt gga atc aag Giy Gly Ilie Lys cct Pro 165 gtt tcc gtc tgg Val Ser Val Trp agc gaa gat tac Ser Giu Asp. Tyr gtc cgc Val Arg 175 gct gca ccc Ala Ala Pro gct tct ttg Ala Ser Leu 195 gga act ggt gac Gly Thr Gly Asp aaa ttt gct ggc Lys Phe Ala Gly aac tac gcg Asn Tyr Ala 190 tgt gac cag Cys Asp Gin ctt gcc cag tcc Leu Ala Gin Ser gct gcg gaa aag Ala Ala Giu Lys ggc Giy 205 gtc gta tgg ttg gat gcc atc gag cac aag tac atc Val Val Trp Leu Asp Ala Ilie Glu His Lys Tyr Ile 210 215 220 gaa gaa atg ggt Glu Glu Met Gly ggc Gly 225 atg aac Ctt ggg Met Asn Leu Giy ttc Phe 230 atc tac cgc aac Ile Tyr Arg Asn gac caa gtc aag Asp Gin Val Lys gtc acc cct gaa Val Thr Pro Glu ctt Leu 245 tcc ggc tca cta Ser Giy Ser Leu cca ggc atc acc Pro Gly Ilie Thr cgc aag Arg Lys 255 tca ctt cta Ser Leu Leu aag atc acc Lys Ile Thr 275 gta gca cgc gac Val Ala Arg Asp gga tac gaa gta Gly Tyr Giu Vai gaa gag cga Giu Glu Arg 270 ggc gcc atg Gly Ala Met acc acc gag tgg Thr Thr Glu Trp, gaa gac gca aag Giu Asp Ala Lys tc t Ser 285 acc gag Thr Giu 290 gca ttt gct tgc Ala Phe Ala Cys act gca gct gtt Thr Ala Ala Val acc cct gtt ggc Thr Pro Val Gly acc Thr 305 gtg aaa tca gct Val Lys Ser Ala c ac His 310 ggc acc ttc gaa Gly Thr Phe Giu aac aac aat gaa Asn Asn Asn Giu WO 01/00843 WO 0100843PCTIiBOO/00923 gga gaa atc acg atg aag ctt cgt gaa acc ctc acc gga att cag caa 1008 Gly Giu Ilie Thr Met Lys Leu Arg Giu Thr Leu Thr Gly Ile Gin Gin 325 330 335 gga aac gtt gaa gac caa aac gga tgg ctt tac cca ctg gtt ggc 1053 Gly Asn Val Giu Asp Gin Asn Giy Trp Leu Tyr Pro Leu Vai Giy 340 345 350 taaatcaacc ggttttaaga ccc 1076 <210> 264 <211> 351 <212> PRT <213> Corynebacterium giutamicun <400> 264 Pro Asp Arg Leu Lys Glu Ile Leu Ala 1 Phe His Thr Tyr Ala Pro Gin Pro Aia 145 Gly Ala Ala 5 Met Leu His Asp Gin Phe Pro Ser Leu Pro 165 Giy Ala Val Val1 Tyr Giu Arg Ile Giu Thr Val1 150 Vai Thr Gin Thr Pro Gly Thr Ser Lys Tyr Giu 135 Ile Ser G ly Ser Aia Trp Pro Ile Thr Arg Glu Giu Leu Pro Leu 170 Lys Ala Lys Giu Pro Giu Arg Ala Leu Ser Val1 140 Gly Giu Ala Lys Gly Glu Asp Ile Asp Pro Asp 110 Tyr Pro Tyr Tyr Asn 190 Cys Val Val Trp, Leu Asp Ala Ile Giu His Lys Tyr Ile Giu Giu Met Giy WO 01/00843 210 Gly Met As 225 Val Thr Pi Ser Leu LE Lys Ile T1~ Thr Glu A] 290 Thr Val Lj 305 Gly Glu I] Gly Asn Ve PCT/iBOO/00923 Leu Glu Gin 260 Thr Phe Ser Thr Glu 340 Gly 235 Pro Tyr Ala Val1 Val1 315 Leu Tyr 220 Asp Gly Glu Lys Ile 300 Asn Thr Pro <210> 265 <211> 1782 <212> DNA <213> Corynebacteriun glutamicum <2 <221> CDS <222> (101)..(1759) <223> RXN01026 <400> 265 gaggtgacat ttatgcctct taatagctat actgtcccat tatagtgaga. tttacagatt tttaaaggac ggtgagttcc gag aac agc acc Glu Asn Ser Thr cgc Arg tac Tyr gac Asp ctg gac Asp atc Ile Gly gcc acc Thr aac Asn gtg Val1 ctg Leu ac c ctg Leu ggc Gly acc Thr cgc Arg gaa gaaacgatat tttaatctca atg acc agc ccc gtg Met Thr Ser Pro Val 1 gca gag aag gtg tgg Ala Glu Lys Val Trp gag ccc'gac ctc ctc Glu Pro Asp Leu Leu tca cca cag gca ttt Ser Pro Gin Ala Phe cac cca gaa ctg cac His Pro Glu Leu His ggc atC aag act ggc acc gaa gac WO 01/00843 Leu Ala Thr tca ctg ctg Ser Leu Leu ctg cgc gac Leu Arg Asp gat gtc cga Asp Val Arg 120 acc cag cca Thr Gin Pro 135 cac ggt gct His Gly Ala 150 gag cac gtc Glu His Val atg gcc att Met Ala Ile gac ctg att Asp Leu Ile 200 ggc tac gtt Gly Tyr Val 215 gat gca cgc Asp Ala Arg 230 gcc ggc atg Ala Gly Met cgc gaa atg Arg Glu Met tgg aag acc Trp Lys Thr 280 gaa atc gat 1027 Glu Ile Asp 295 Giu gaa Glu aac Asn 105 cag Gin Giy ttt Phe a tg Met gaa Giu 185 ctg Leu ctg Leu atg Met atc Ile gc a Al a 265 ctg Leu ggc Gly Asp atc Ile tgt Cys ggc Gly atg Met ggC Giy gc t Aia 170 gt t Val gcg Al a gaa Glu a cc Thr gcc Ala 250 c ca Pro cca Pro tcg Ser Glu 80 cgc Arg cgc Arg ggC Gly gac Asp atc Ile 160 ctg Leu cca Pro ggC Gly atc Ile atc Ile 240 ttc Phe gac Asp acc Thr a tc Ile Gly ctg Leu ctg Leu cca Pro t cc Ser 145 ggt G ly aag Lys ggt Giy ac c Thr cgt Arg 225 gaa Giu gac Asp gaa Glu ttt Phe acc Thr 305 PCTLBOO/00923 Thr Gly tct act 403 Ser Thr 100 atg ggt 451 Met Gly ggc gca 499 Gly Ala tcc acc 547 Ser Thr gag gtt 595 Glu Val 165 aag acc 643 Lys Thr 180 tcc aag 691 Ser Lys gga cag 739 Gly Gin tcc atg 787 Ser Met gca cgt 835 Ala Arg 245 gaa ggc 883 Glu Gly 260 gct tac 931 Ala Tyr gtc gta 979 Val Val acc aac Thr Asn WO 01/00843 WO 0100843PCT/EBOO/00923 cca ggc 1075 Pro Gly 310 ttc acc 1123 Phe Thr atg gac 1171 Met Asp gtc ttc 1219 Val Phe gcc gct 1267 Ala Ala 375 atg gtc 1315 Met Val 390 gga ctg 1363 Gly Leu ggc tgc 1411 Gly Cys gag cgc 1459 Glu Arg cag ggc ctg Gin Gly Leu aac gac aac Asn Asp Asn 330 ctg gta cca Leu Val Pro 345 ctg gga tcc Leu Gly Ser cca ctg ggc gaa tcc gta Pro Leu Gly Giu Ser Val 315 320 gac aag gca gca gcc gaa Asp Lys Ala Ala Ala Glu 335 gga acc cca ctg cgc gac Gly Thr Pro Leu Arg Asp 350 tgc acc aac gcc cgc atc Cys Thr Asn Ala Arg Ile 365 aag ggc cac aaa atc gcc Lys Gly His Lys Ile Ala cca agc cca gaa gac Pro Ser Pro Giu Asp 325 aag gca ctg cag tac Lys Ala Leu Gin Tyr 340 atc aag atc gac acc Ile Lys Ile Asp Thr 355 gaa gac ctg cag atc Glu Asp Leu Gin Ile 370 gac ggc atg cgc atg Asp Gly Met Arg Met 360 gac Asp atc ctc Ile Leu 380 385 gtg cct tcc tcc Vai Pro Ser Ser 395 gac aaa atc ttc Asp Lys Ile Phe 410 acc tgg atc aag caa Thr Trp Ile Lys Gin 400 acc gac gct ggc gct Thr Asp Ala Gly Ala gag gca gaa gcg ctc Giu Ala Glu Ala Leu 405 gaa tgg cgt acc gca Glu Trp Arg Thr Ala 420 tcc atg Ser Met 425 tcc gca Ser Ala tgc ctg ggc atg aac Cys Leu Gly Met Asn 430 ttc acc tcc aac cga Phe Thr Ser Asn Arg 445 cca gac caa ctg aag cca ggc Pro Asp Gin Leu Lys Pro Gly 435 aac ttc gaa gga cgc caa gga Asn Phe Glu Gly Arg Gin Gly 450 440 cca gga ggc 1507 Pro Gly Gly 455 gaa tcc gcg 1555 Giu Ser Ala 470 aaa caa tgg 1603 Lys Gin Trp cgc Arg acc cac ctg Thr His Leu 460 gac cct gtc ctc Asp Pro Val Leu gta tcc cca gca gtc Val Ser Pro Ala Val 465 acc tgc aga tat cta Thr Cys Arg Tyr Leu 480 cct aca ccg gcg ttg Pro Thr Pro Ala Leu 495 gca goc gcc acc Ala Ala Ala Thr agg aag gct aga Arg Lys Ala Arg 485 gcg ttc cac tgc Ala Phe His Cys 500 aaa aat Lys Asn 490 475 tta cca Leu Pro WO 01/00843 agc gat cca acg tgg aca ccg acc aga tca tcc 1651 Ser Asp Pro Thr Trp, Thr Pro Thr Arg Ser Ser 505 510 agc gcg tca ccc gga ccg gct tcg aag acg gac 1699 Ser Ala Ser Pro Gly Pro Ala Ser Lys Thr Asp 520 525 gcc aaa acg acc cca act ttg tcc tca aca ccg 1747 Ala Lys Thr Thr Pro Thr Leu Ser Ser Thr Pro 535 540 gct ccg ttc tcg tagcaggccc tgactttggc acc 1782 Ala Pro Phe Ser 550 <210> 266 <211> 553 <212> PRT <213> Corynebacterium giutamicum cag ccg tct Gin Pro Ser 515 tgt ttt cca Cys Phe Pro 530 aca cct aca Thr Pro Thr 545 PCTIB00100923 acc tca Thr Ser act ggc Thr Gly aga acg Arg Thr <400> 266 Met Thr Ser Pro Val Glu Asn Ser Thr 1 Ala Glu Ser His Gly Leu Leu Pro Ser 145 Gly Lys Asp Gin Giu Lys Val Pro 115 Leu Thr Ser Val1 Leu Ala Leu Thr Ser 100 Met Gly Ser Glu 5 Trp, Leu Phe His Gly Thr Gly Al a Thr Val Arg Tyr Asp Leu 70 S er Leu Asp Thr His 150 Glu Asp Ile Gly Ala Leu Arg Val Gin 135 Gly His His Asp Leu Thr Leu Asp Arg 120 Pro Ala Val1 Val1 Leu Arg Giu Giu Asn 105 Gin Gly Phe Met Ser 10 Val1 Gin Met Asp Ile 90 Cys Gly Met Gly Ala 170 Val1 Thr Ser Leu Thr His 75 Asn Giu Ile Thr Ser 155 Thr Thr Glu Lys Leu Gly Asn Asp Giu Val1 Ile 140 Met Gin Gly Thr Asn Val1 Leu Thr Ser Val Val Gly Gly Pro 175 Gin Leu Gly Thr Arg Glu Arg Arg Gly Asp Ile 160 Leu Pro 165 Lys Pro Phe Lys Thr Met Ala Ile Giu WO 01/00843 WO 0100843PCTIiBOO/00923 180 Ser Giy Ser Ala Giu 260 Ala Val1 Thr Giu Gin 340 Asp Gin Arg Ala Thr 420 Pro Gin Ala Al a His 500 Lys Gin Met Arg 245 Gly Tyr Val1 Asn Asp 325 Tyr Thr Ile Met Leu 405 Ala Gly Gly Thr Arg 485 Cys Ile Arg 220 Cys Asp Gly Asp Leu 300 Leu Lys Thr Thr Gly 380 Thr Thr Giy Ser Leu 460 Leu Pro Pro Ala 205 Gly Asn Gin Ala Glu 285 Thr Gly Ala Pro Asn 365 His Trp Asp Met Asn 445 Val1 Thr Pro Thr 190 Lys Glu Met Thr Asp 270 Gly Pro Giu Ala Leu 350 Al a Lys Ile Aia Asn 430 Arg Ser Cys Thr Arg 510 Ile Ala Ser Thr 255 Trp Ala Phe Ser Ala 335 Arg Arg Ile Lys Gly 415 Pro Asn Pro Arg Pro 495 Ser WO 01/00843 WO 0100843PCTJIBOO/00923 Gin Pro Ser Thr Ser Ser Ala Ser Pro 515 520 Gly Pro Ala Ser 525 Lys Thr Asp Cys Phe Pro Thr Gly Ala Lys Thr Thr Pro Thr Leu 530 535 540 Thr Pro Thr Arg Thr Ala Pro Phe Ser 545 550 Ser Ser Thr Pro <210> <211> <212> <213> <220> <221> <222> <223> 267 1625

DNA

Corynebacteriun giutanicun

CDS

(1602) FRXA01026 <400> 267 gtg tgg cgc gac cat Val Trp, Arg Asp His 1 gtc gtg tcc aag gga gaa aac ggc gag ccc gac Val Val Ser Lys Gly Glu Asn Gly Glu Pro Asp ctc ctc tac Leu Leu Tyr gac ctg cag ctg ctg cat gaa gtg acc Asp Leu Gin Leu Leu His Glu Val Thr tca cca cag Ser Pro Gin cac cca gaa His Pro Glu gca ttt gac ggc ctg cgc atg acc ggc cgt aaa ctg Ala Phe Asp Gly Leu Arg Met Thr Gly Arg Lys Leu cgc Arg ctg cac ctg gcc acc gaa Leu His Leu Ala Thr Giu cac aac gtg cca acc gaa ggc atc aag His Asn Val Pro Thr Glu Gly Ile Lys act Thr ggc tca ctg ctg Gly Ser Leu Leu gaa Glu 70 atc aac gac aag Ile Asn Asp Lys att Ile tcc cgc ctg cag Ser Arg Leu Gin 240 288 tct act ctg cgc Ser Thr Leu Arg aac tgt gaa gaa Asn Cys Giu Giu ggc gtg cgc ctg Gly Val Arg Leu cac cca His Pro atg ggt gat Met Gly Asp ggc gca acc Gly Aia Thr 115 cga cag ggc atc Arg Gin Giy Ile gtg Val 105 cac acc gtc ggc His Thr Val Giy cca cag ctc Pro Gin Leu 110 tcc cac acc Ser His Thr cag cca ggc atg Gin Pro Gly Met acc Thr 120 att gtg tgc ggt Ile Vai Cys Giy tcc acc Ser Thr 130 cac ggt gct ttt His Gly Ala Phe ggc Gly 135 tcc atg gca ttc Ser Met Ala Phe ggc Gly 140 atc ggt acc tca Ile Gly Thr Ser gag Giu 145 gtt gag cac gtc Val Glu His Val gct act caa acc Ala Thr Gin Thr cca ctg aag cct Pro Leu Lys Pro t tc Phe 160 WO 01/00843 WO 0100843PCTIIBOO/00923 aag acc Lys Thr tcc aag Ser Lys gga cag Giy Gin tcc atg Ser Met 210 gca cgt Ala Arg 225 gaa ggc Giu Gly gct tac Ala Tyr gtc gta Val Val acc aac Thr Asn 290 gaa gac Glu Asp 305 cag tac 1008 Gin Tyr gac acc 1056 Asp Thr cag atc 1104 Gin Ile cgc atg 1152 Arg Met 370 atg Met gac Asp ggc Giy 195 ga t Asp gcc Al a cgc Arg tgg Trp gaa Glu 275 cca Pro ttc Phe atg Met gtc Val1 gcc Aia 355 atg Met gcc Ala c tg Leu 180 tac Tyr gca Ala ggC Gly gaa Glu aag Lys 260 atc Ile ggc Gly acc Thr gac Asp ttc Phe 340 gc t Ala gtc Val1 ggt Gly atc Ile 185 cgc Arg tgc Cys gac Asp ggc G ly gac Asp 265 ctg Leu ctg Leu aag Lys acc Thr acc Thr 345 ggc Gly acc Thr gaa Giu 170 gcc Al a ggc Gly aac Asn caa Gin gcc Ala 250 gaa Glu acc Thr ggC Gly gca Ala cca Pro 330 aac Asn cac His tgg Trp ctg Leu aag Lys gaa Glu atg Met acc Thr 235 gac Asp ggC Giy cca Pro gaa Glu gca Ala 31.5 ctg Leu gcc Ala a aa Lys atc Ile cag Gin atc Ile gca Ala tcc Ser 220 acc Thr tgg Trp gca Aia t tc Phe tcc Ser 300 gcc Ala cgc Arg cgc Arg atc Ile aag Lys 380 528 576 624 672 720 768 816 864 912 960 WO 01/00843 WO 0100843PCTAIBOO/00923 gcg ctc 1200 Ala Leu 385 acc gca 1248 Thr Ala cca ggc 1296 Pro Gly caa gga 1344 Gin Gly gcc acc 1392 Ala Thr 450 gct aga 1440 Ala Arg 465 cac tgc 1488 His Cys acc tca 1536 Thr Ser act ggc 1584 Thr Gly aga acg 1625 Arg Thr 530 gga ctg gac Gly Leu Asp ggc tgc tcc Gly Cys Ser aaa atc ttc acc gac gct Lys Ile Phe Thr Asp Ala 390 395 atg tgc ctg ggc atg aac Met Cys Leu Gly Met Asn 410 gca ttc acc tcc aac cga Ala Phe Thr Ser Asn Arg 425 cgc acc cac ctg gta tcc Arg Thr His Leu Val Ser gag cgc Giu Arg 420 cca gga Pro Gly ggc gct gaa tgg cgt Gly Ala Giu Trp Arg 400 cca gac caa ctg aag Pro Asp Gin Leu Lys 415 aac ttc gaa gga cgc Asn Phe Glu Gly Arg 430 cca gca gtc gca gcc Pro Ala Val Ala Ala 445 aga tat cta agg aag Arg Tyr Leu Arg Lys 460 ccg gcg ttg gcg ttc Pro Ala Leu Ala Phe 480 ggC G ly 435 gaa Glu aaa Lys 440 tcc gcg gac Ser Ala Asp caa tgg aaa Gin Trp Lys cct Pro 455 gtc Val1 ctc acc tgc Leu Thr Cys 470 agc gat cca acg Ser Asp Pro Thr aat tta cca Asn Leu Pro tgg aca ccg Trp Thr Pro cct aca Pro Thr 475 agc Ser gcc Al a gcg tca ccc gga ccg gct Ala Ser Pro Gly Pro Ala 500 505 aaa acg acc cca act ttg Lys Thr Thr Pro Thr Leu acc aga tca tcc cag ccg tct Thr Arg Ser Ser Gln Pro Ser 490 495 tcg aag acg gac tgt ttt cca Ser Lys Thr Asp Cys Phe Pro 510 tcc tca aca ccg aca cct aca Ser Ser Thr Pro Thr Pro Thr 515 gct ccg ttc tcg Ala Pro Phe Ser tagcaggccc tgactttggc acc <210> 268 <211> 534 <212> PRT <213> Corynebacterium glutamicum <400> 268 Val Trp Arg Asp His Val Val Ser Lys Gly Glu Asn Gly Glu Pro Asp 1 5 10 Leu Leu Tyr Ile Asp Leu Gin Leu Leu His Glu Val Thr Ser Pro Gin WO 01/00843 WO 0100843PCTIIIBOO/00923 Ala Phe Asp Leu His Leu Thr Gly Ser Ser Thr Leu Met Gly Asp Gly Ala Thr 115 Ser Thr His 130 Glu Val Glu 145 Lys Thr Met Ser Lys Asp Gly Gin Gly 195 Ser Met Asp 210 Ala Arg Ala 225 Glu Gly Arg Ala Tyr Trp Val Val Glu 275 Thr Asn Pro 290 Glu Asp Phe 305 Gin Tyr Met Asp Thr Val Gly Leu Arg Met Thr Gly Arg Lys Leu Glu Glu Asn Gin Gly Phe Met 150 Giu Leu Leu Met Ilie 230 Ala Leu Gly Gly Asp 310 Val1 Gly Asp Ile Cys Gly Met Gly 135 Ala Val Ala Glu Thr 215 Ala Pro Pro S er Leu 295 Asn Pro Ser Arg Glu Arg Arg Gly Asp 125 Ile Leu Pro Gly Ile 205 Ile Phe Asp Thr Ile 285 Val Glu

ASP

Ile His Gly Leu Leu Pro 110 Ser Gly Lys Gly Thr 190 Arg Glu Asp Glu Phe 270 Thr Pro Lys Ile Glu 350 WO 01/00843 WO 0100843PCT/IBOO/00923 Gin Ile Ala Ala Asp Ile Leu Lys Gly His Lys Ile Ala Asp Gly Met 355 360 365 Arg Met Met Val Val Pro Ser Ser Thr Trp Ile Lys Gin Glu Ala Glu 370 375 380 Ala Leu Gly Leu Asp Lys Ile Phe Thr Asp Ala Gly Ala Giu Trp Arg 385 390 395 400 Thr Ala Gly Cys Ser Met Cys Leu Gly Met Asn Pro Asp Gin Leu Lys 405 410 415 Pro Giy Giu Arg Ser Ala Phe Thr Ser Asn Arg Asn Phe Giu Gly Arg 420 425 430 Gin Giy Pro Gly Gly Arg Thr His Leu Val Ser Pro Ala Val Ala Ala 435 440 445 Ala Thr Glu Ser Ala Asp Pro Val Leu Thr Cys Arg Tyr Leu Arg Lys 450 455 460 Ala Arg Lys Gin Trp Lys Asn Leu Pro Pro Thr Pro Ala Leu Ala Phe 465 470 475 480 His Cys Ser Asp Pro Thr Trp Thr Pro Thr Arg Ser Ser Gin Pro Ser 485 490 495 Thr Ser Ser Ala Ser Pro Gly Pro Ala Ser Lys Thr Asp Cys Phe Pro 500 505 510 Thr Gly Ala Lys Thr Thr Pro Thr Leu Ser Ser Thr Pro Thr Pro Thr 515 520 525 Arg Thr Ala Pro Phe Ser 530 <210> 269 <211> 1143 <212> DNA <213> Corynebacterium giutamicum <22 0> <221> CDS <222> (101)..(1120) <223> RXN01127 <400> 269 gccctgcatg atggggtagt gggggttgtt gggcaggtac gagctgtgat caatcagcta cactagtgaa gtccatatag tgagaaggga atcccacaac atg aaa ctt gct gtt 115 Met Lys Leu Ala Val 1 att ggt gga gat ggt atc ggc cca gag gtt act gca gaa gcc ctc aag 163 Ile Gly Gly Asp Gly Ile Gly Pro Giu Val Thr Ala Glu Ala Leu Lys 15 gtt cta aac gct gtc cgc gac gac atc gag acc acc gat tat gac ctt 211 Val Leu Asn Ala Val Arg Asp Asp Ile Giu Thr Thr Asp Tyr Asp Leu WO 01/00843 WO 0100843PCTIIBOO/00923 ggc Gly gca cgc cgt tac ctc aaa aat ggc gag ctg ctc Ala Arg Arg Tyr Leu Lys Asn Gly Giu Leu Leu 45 acc Thr gac gag gat Asp Giu Asp ctg gca Leu Ala tcc ctg cgc gag Ser Leu Arg Giu gac gcg atc ctt Asp Ala Ile Leu ggc gct atc ggt Gly Ala Ile Gly gca Ala cca ggt tcc gtc Pro Gly Ser Val cct Pro cca gga att ctc Pro Gly Ile Leu cgt ggt ttg ctg Arg Gly Leu Leu aag atg cga ttc Lys Met Arg Phe gca Ala ctg gat cac cac Leu Asp His His aac ctg cgc cca Asn Leu Arg Pro tcc aag Ser Lys 100 ctg tac gac Leu Tyr Asp ttc gtt gtg Phe Val Val 120 gtg gag tcc cca ctg cgt aac cca ggc Val Giu Ser Pro Leu Arg Asn Pro Gly 110 aag att gat Lys Ile Asp 115 ggc aac ggt Gly Asn Gly gtc cgc gaa ggt Val Arg Giu Gly gaa ggc gcc tac Giu Gly Ala Tyr act Thr 130 gga gca Gly Ala 135 atc cgc gtg gga acc cct cac gag att Ilie Arg Val Gly Thr Pro His Giu Ilie 140 aat gaa acc tcc Asn Glu Thr Ser aac act cgc tac Asn Thr Arg Tyr gct gag cgc gtt Ala Glu Arg Val cgc tac gca ttc Arg Tyr Ala Phe ctg gca cag agc Leu Ala Gin Ser cgc Arg 170 cgc aag aag ctc Arg Lys Lys Leu ctc gtg cac aag Leu Val His Lys acc aac Thr Asn 180 gtc ctg gtt Val Leu Val gca aag gaa Ala Lys Glu 200 ggt ggt ggc ctg Gly Gly Gly Leu cag cgc acc gta Gin Arg Thr Val gat gag gtt Asp Giu Val 195 atc gat gca Ile Asp Ala tac cca gag gta Tyr Pro Giu Val gtc gat tac aac Val Asp Tyr Asn gca acc Ala Thr 215 atc tat ctg gtc Ilie Tyr Leu Val gat cct tcc cgc Asp Pro Ser Arg gat gtg att gtt Asp Val Ile Val gat aac ctc ttc Asp Asn Leu Phe ggc Gly 235 gac atc ctc acc Asp Ile Leu Thr gag gca ggc gca Glu Ala Giy Ala tct ggc gga att Ser Gly Gly Ile ggc Gly 250 ctc gca gca tcc Leu Ala Ala Ser aac atc gat gcc Asn Ile Asp Ala acg ggc Thr Gly 260 acc aac cct Thr Asn Pro tcc Ser 265 atg ttc gag cca Met Phe Giu Pro cac ggc tct gca His Gly Ser Ala cca gat atc Pro Asp Ile 275 WO 01/00843 WO 0100843PCT/IBOOIOO923 gca ggc cag gga atc gca gac cca acg Ala Gly Gin Gly Ile Ala Asp Pro Thr 280 285 atg ctg ctg cgt cac tta ggt gat gaa 1027 met Leu Leu Arg His Leu Giy Asp Glu 295 300 aca gcc atc gca gct gat gtg gct ggc 1075 Thr Ala Ilie Ala Ala Asp Val Ala Giy 310 315 tct acc act gag gtg gga gac cgc atc 1120 Ser Thr Thr Glu Val Giy Asp Arg Ile 330 taaatttcaa cgccgacccc ctt 1143 <210> 270 <211> 340 <212> PRT <213> Corynebacterium glutamicum <400> 270 Met Lys Leu Ala Val Ile Gly Gly Asp gca Ala gac Asp cga Arg gtc Val 335 Gly 10 Val Tyr Arg Val1 Ala 90 Val1 Arg Val Tlyr Arg tcc Ser cgt Arg cag Gin caa Gin Glu Ile Gly Ala Ile His Leu 110 Glu His Arg Leu gc t Aia att Ile ccg Pro agc Ser 340 Val Giu Glu Ile Leu Val Arg Gly Glu Val Thr gcg 979 Ala gaa Giu att.

Ile 325 Thr Thr Leu Leu Giu Asn Asfl Ala Ile Ile 160 Leu 1 Ala Thr Leu Leu Arg Leu Pro Tyr Ala 145 Arg Leu Asp Giu Ile Leu Ser 100 Ile Asn Thr Phe 5 Lys Leu Asp Gly Leu Lys Asp Gly Ser Glu Val Gly Leu Ala 70

LYS

Leu Phe Gly Val 150 Leu Leu Ala Ala Pro Met Tyr Val1 Ala 135 Asn Ala Asn Arg Ser Gly Arg Asp Val 120 Ile Thr Gin WO 01/00843 Lys Asp 195 Ile Val Gly Ala Pro 275 Ser Arg Gin Gin Thr 180 Glu Asp Ile Ala Thr 260

ASP

Ala Ile Pro Ser 340 165 Asn Val Ala Val Val 245 Gly Ile Ala Glu Ile 325 Val Ala Ala Thr 230 Ser Thr Aia Met Thr 310 Ser 170 Giy Pro Leu Phe Gly 250 Met Ile His Ala Vali 330 Gly Giu Val Gly 235 Leu Phe Ala Leu Asp 315 Gly Gly Val Thr 220 Asp Ala Glu Asp Gly 300 Val Asp Trp, 190 Val Pro Leu Ser Val1 270 Thr Giu Giy Ile PCT/TBOO/00923 i7 Gin Arg Asp Tyr Ser Arg Thr Asp 240 Gly Asn 255 His Giy Aia Aia Asp Asn Arg Asp 320 Val Lys 335 <210> 271 <211> 403 <212> DNA <213> Corynebacterium glutamicun <22 0> <22i> CDS <222> (101)..(403) <223> FRXA01132 <400> 271 gccctgcatg atggggtagt gggggttgtt gggcaggtac gagctgtgat caatcagcta cactagtgaa gtccatatag tgagaaggga atcccacaac atg aaa ctt gct gtt 115 Met Lys Leu Ala Val 1 att ggt gga gat ggt atc ggc cca gag gtt act gca gaa gcc ctc aag 163 Ile Gly Gly Asp Gly Ile Gly Pro Glu Val Thr Ala Giu Aia Leu Lys 15 gtt cta aac gct gtc cgc gac gac atc gag acc acc gat tat gac ctt 211 Val Leu Asn Ala Val Arg Asp Asp Ile Giu Thr Thr Asp Tyr Asp Leu 30 WO 01/00843 PCTIBOO/00923 ggc gca cgc cgt tac ctc aaa aat ggc gag ctg ctc acc gac gag gat 259 Gly Ala Arg Arg Tyr Leu Lys Asn Gly Giu Leu Leu Thr Asp Glu Asp 45 ctg gca tcc ctg cgc gag cat gac gcg atc ctt ctt ggc gct atc ggt 307 Leu Ala Ser Leu Arg Glu His Asp Ala Ile Leu Leu Gly Ala Ile Gly 60 gca cca ggt tcc gtc cct cca gga att ctc gag cgt ggt ttg Ctg ctg 355 Ala Pro Gly Ser Val Pro Pro Gly Ilie Leu Glu Arg Gly Leu Leu Leu 75 80 aag atg cga ttc gca ctg gat cac cac gtg aac ctg cgc cca tcc aag 403 Lys Met Arg Phe Ala Leu Asp His His Val Asn Leu Arg Pro Ser Lys 95 100 <210> 272 <211> 101 <212> PRT <213> Corynebacteriun glutamicum <400> 272 Met Lys Leu Ala Val Ile Gly Gly Asp Gly Ile Gly Pro Glu Val Thr 1 5 10 Ala Glu Ala Leu Lys Val Leu Asn Ala Val Arg Asp Asp Ile Glu Thr 25 Thr Asp Tyr Asp Leu Gly Ala Arg Arg Tyr Leu Lys Asn Gly Glu Leu 40 Leu Thr Asp Glu Asp Leu Ala Ser Leu Arg Glu His Asp Ala Ile Leu 55 Leu Gly Ala Ile Gly Ala Pro Gly Ser Val Pro Pro Gly Ile Leu Glu 70 75 Arg Gly Leu Leu Leu Lys Met Arg Phe Ala Leu Asp His His Val Asn 90 Leu Arg Pro Ser Lys 100 <210> 273 <211> 1494 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (101)..(1471) <223> RXN00536 <400> 273 cggcgggtcc cagaggtctt aacacgaccg gcatcccgtc gcggagtttg gtgttgccgg tcgtggaccc acccaaaact ttttaagaag gttgaacaca atg tct cct aac gat 115 Met Ser Pro Asn ASP 1 WO 01/00843 WO 0100843PCTJIBOO/00923 gca tec aec tcc gca cct gcc aag atc Ala Phe Ile Ser Ala Pro Ala Lys Ilie &cc cca get ggg Thr Pro Val Gly cct cgc Pro Arg aac gaa ggc Asn Giu Gly get: aac cgc Val Asn Arg cca gca egg aae Pro Ala Trp Asn cag cgt ggc tcc tca atg cca Gin Arg Giy Ser Ser Met Pro tac aeg cct etc gag gee gag gta gaa Tyr Met Pro Phe Giu Val Glu Val Glu ate ece ceg Ile Ser Leu ccg gac Pro Asp cgc act tgg cca Arg Thr Trp Pro aaa aaa atc acc Lys Lys Ile Thr gca cct cag egg Ala Pro Gin Trp 211 259 307 355 403 gct get gac ceg Ala Val Asp Leu gac ggc aac cag Asp Gly Asn Gin gc t Ala ceg ate gat ccg Leu Ile Asp Pro ect cct gag cgt Ser Pro Glu Arg aag Lys cgc cgc aeg tee Arg Arg Met Phe ctg ceg gtt cag Leu Leu Val Gin aeg ggc Met Gly 100 tec aaa gaa Phe Lys Giu gat etc gtt Asp Phe Vai 120 gag gec ggt etc Glu Val Gly Phe eca gct tcc cag Ser Ala Ser Gin act gat ttt Thr Asp Phe 115 cgt gag atc aec Arg Glu Ile Ile gaa aag ggc atg aec cct gac gae gec Giu Lys Gly Met Ile Pro Asp Asp Val 125 130 gct cge gag cac ceg aet cgc cgt ace Ala Arg Glu His Leu Ile Arg Arg Thr acc ate Thr Ile 135 cag gte ceg gt Gin Val Leu Val gaa gct egc gaa Giu Ala Cys Giu gca aaa aac get atc geg cac etc tac Ala Lys Asn Val Ile Val His Phe Tyr 160 tcc acc tcc aec Ser Thr Ser Ilie cag gtg aagaag Gin ValLys Lys 185 gct cag gat tac Ala Gin Asp Tyr 200 cag cgc aac geg Gin Arg Asn Val etc cgc atg gac Phe Arg Met Asp aag geg Lys Val 180 ceg gct acc gat Leu Ala Thr Asp gct gaa cta atc Ala Giu Leu Ile aag acc aec Lys Thr Ile 195 tcc cce gag Ser Pro Glu 691 739 cca gac acc Pro Asp Thr egg cgc egg cag Trp Arg Trp Gin ecc eec acc ggc act gag gt Ser Phe Thr Giy Thr Giu Val 215 220 gag tac gcc aag Giu Tyr Ala Lys get geg gac gca Val Val Asp Ala gee gag gec atg Val Glu Val Met cca ace cce gag aac cca aeg aec aec Pro Thr Pro Glu Asn Pro Met Ile Ile 240 aac Asn 245 835 WO 01/00843 ctg cct tcc acc Leu Pro Ser Thr gag atg atc acc Giu Met Ile Thr cct Pro 255 cgt Arg aac gtt tac gca Asn Val Tyr Ala PCTIBOOOO923 gac tcc 883 Asp Ser 260 atc ctg 931' Ile Leu gct gag 979 Ala Giu att gaa tgg Ile Giu Trp tcc ctg cac Ser Leu His 280 ctg ggc tac 1027 Leu Gly Tyr 295 aac ggc gag 1075 Asn Gly Glu 310 atg ctg acc 1123 Met Leu Thr cgc aat cta Arg Asn Leu aac Asn 270 cgt gat tcc Arg Asp Ser att Ile 275 cac aat gac His Asn Asp atg gct ggc gct Met Ala Gly Ala 300 cgc acc ggc aac Arg Thr Gly Asn 315 ggc acc ggc gtt Gly Thr Gly Val cgc atc gaa ggc Arg Ile Giu Gly 305 tgc ctg gtc acc Cys Leu Val Thr 290 tgc ctg ttc ggc Cys Leu Phe Gly ctg gca ctg aac Leu Ala Leu Asn 325 ggc gca Gly Ala gtc Val 320 cag ggc Gin Gly 330 gtt gac cct cag Val Asp Pro Gin gtt gaa tac tgc Val Giu Tyr Cys c tg Leu 335 gac Asp ttc acc gat ata cgc Phe Thr Asp Ile Arg 340 cag atc 1171 Gin Ile cgc cac 1219 Arg His cac cag 1267 His Gin 375 cag cca 1315 Gin Pro 390 acc cga 1363 Thr Arg tcg cga 1411 Ser Arg cgg cgt 1459 Arg Arg cgc agc Arg Ser 345 cca tac Pro Tyr ac c Thr aac cag ctg cgc gtt cct gag Asn Gin Leu Arg Val Pro Glu 355 ttc acc gct ttc tcc ggt tcc Phe Thr Ala Phe Ser Gly Ser 370 360 gac Asp ggc ggt gac ctg Gly Gly Asp Leu 365 gtg aac aag ggt Val Asn Lys Gly gc t Ala ctg gac gcc atg gct gcc aag gtt Leu Asp Ala Met Ala Ala Lys Val 385 380 ggt gct agc tcc Gly Ala Ser Ser 395 atg gga ggt tcc Met Gly Gly Ser 410 cta cga ggc tgt Leu Arg Gly Cys act gaa gtt tct tgg Thr Glu Val Ser Trp 400 tta cct gcc tat cga Leu Pro Ala Tyr Arg 415 tat ccg cgt gaa ctc- Tyr Pro Arg Giu Leu gag cag ctg cgc gac Glu Gin Leu Arg Asp 405 tcc aaa gga tgt cgg Ser Lys Gly Cys Arg 420 cca gtc cgg caa ggg Pro Val Arg Gin Gly 435 tct gca gat ccc tcg Ser Ala Asp Pro Ser 450 425 tgc tta Cys Leu 440 430 cat cat gaa gac cga His His Giu Asp Arg S445 tca cgg Ser Arg WO 01/00843 WO 0100843PCTIIBOO/00923 ctc cat gca ggt tgagttctcc accgttgtcc aga 1494 Leu His Ala Gly 455 <210> 274 <211> 457 <212> PRT <213> Corynebacterium giutamicum <400> 274 Met Ser Pro Asn Asp Ala Phe Ile Ser Ala Pro Ala Lys Ile Giu Thr 1 5 10 Pro Val Gly Pro Arg Asn Giu Gly Gin Pro Ala Trp Asn Lys Gin Arg 25 Gly Ser Ser Met Pro Val Asn Arg Tyr Met Pro Phe Giu Val Giu Val 40 Giu Asp Ile Ser Leu Pro Asp Arg Thr Trp Pro Asp Lys Lys Ile Thr 55 Val Ala Pro Gin Trp Cys Ala Val Asp Leu Arg Asp Giy Asn Gin Ala 70 75 Leu Ilie Asp Pro Met Ser Pro Giu Arg Lys Arg Arg Met Phe Glu Leu 90 Leu Val Gin Met Gly Phe Lys Glu Ile Giu Val Giy Phe Pro Ser Ala 100 105 110 Ser Gin Thr Asp Phe Asp Phe Val Arg Gu Ile Ile Giu Lys Gly Met 115 120 125 Ile Pro Asp Asp Val Thr Ile Gin Val Leu Val Gin Ala Arg Giu His 130 135 140 Leu Ile Arg Arg Thr Phe Giu Ala Cys Giu Gly Ala Lys Asn Val Ile 145 150 155 160 Val His Phe Tyr Asn Ser Thr Ser Ile Leu Gin Arg Asn Val Vai Phe 165 170 175 Arg Met Asp Lys Val Gin Val Lys Lys Leu Aia Thr Asp Ala Aia Giu 180 185 190 Leu Ile Lys Thr Ilie Ala Gin Asp Tyr Pro Asp Thr Asn Trp Arg Trp 195 200 205 Gin Tyr Ser Pro Giu Ser Phe Thr Gly Thr Giu Val Giu Tyr Ala LYS 210 215 220 Glu Val Val Asp Ala Val Val Giu Val Met Asp Pro Thr Pro Glu Asn 225 230 235 240 Pro Met Ile Ile Asn Leu Pro Ser Thr Val Glu Met Ile Thr Pro Asn 245 250 255 WO 01/00843 Val1 Tyr Ali Asp Ser IlE 27! Val Gly Ali 290 Gly Cys Lei 305 Thr Leu Ali Phe Thr As; Leu Arg Va& Ala Phe Sej 370 Met Ala Ali 385 Giu Gin Lei Ser Lys Gl: Pro Val Arl 43! Ser Ala As] 450 <210> 275 <211> 1333 <212> DNA Ie Ser Leu Asn 310 Met Gin Arg His Gin 390 Thr Ser Arg Leu Giu Leu Gly 295 Gly Leu Ile His Gin 375 Pro Arg Arg Arg His 455 Trp, His 280 Tyr Giu Thr Arg Pro 360 Asp Gly Met Leu Cys 440 Ala Asn 270 Giy Arg Cys Gin Cys 350 Val Leu Val Ala Arg 430 Arg PCTJIBOO/00923 Arg Arg Thr Gly Ile Glu Leu Vai 320 Leu Asp 335 Asn Gin Phe Thr Asp Ala Ser Trp 400 Tyr Arg 415 Giu Leu Ser Arg <213> Corynebacterium glutamicumr <220> <221> CDS <222> (101)..(333) <223> FRXA00536 <400> 275 cggcgggtcc cagaggtctt aacacgaccg gcatcccgtc gcggagtttg gtgttgccgg tcgtggaccc acccaaaact ttttaagaag gttgaacaca atg tct cct aac gat 115 Met Ser Pro Asn Asp 1 gca ttc atc tcc gca cct gcc aag atc gaa acc cca gtt ggg cct cgc 163 Ala Phe Ile Ser Ala Pro Ala Lys Ilie Glu Thr Pro Val Gly Pro Arg 15 WO 01/00843 aac gaa ggc Asn Giu Gly gtt aac cgc Val Asn Arg ccg gac cgc Pro Asp Arg tgt gct gtt Cys Ala Val tct cct gag Ser Pro Giu ttc aaa gaa Phe Lys Giu gat ttc gtt Asp Phe Val 120 acc att cag Thr Ile Gin 135 ttt gaa gct Phe Giu Ala 150 tcc acc tcc Ser Thr Ser cag gtg aag Gin Val Lys gct cag gat Ala Gin Asp 200 tcc ttc acc Ser Phe Thr 215 gtt gtt gag Val Val Giu 230 ctg cct tcC Leu Pro Ser gca Ala cc t Pro c ca Pro cgt Arg 75 cgc Arg gtc Val atc Ile gt t Val ggc Gly 155 cag Gin gc t Ala gac Asp gag Giu ga t Asp 235 gag Glu tgg Trp t tc Phe ga t Asp 60 gac Asp cgc Arg ggt Gly atc Ile cag Gin 140 gca Ala cgc Arg acc Thr acc Thr gt t Val 220 cca Pro atg Met aat Asn gag Giu 45 aaa Lys ggc Gly atg Met t tc Phe gaa Giu 125 gc t Aia aaa Lys aac Asn gat Asp aac Asn 205 gag Giu act Thr atc Ile aag cag Lys Gin gtt gag Val Giu aaa atc Lys Ile aac cag Asn Gin ttt gag Phe Giu cct tca Pro Ser 110 aag ggc Lys Gly cgt gag Arg Giu aac gtt Asn Val gtg gtg Vai Val 175 gcc gct Ala Ala 190 tgg cgc Trp Arg tac gcc Tyr Ala cct gag Pro Giu acc cct Thr Pro 255 cgt Arg gta Val1 acc Thr gc t Ala 80 c tg Leu gc t Ala atg Met cac His atc Ile 160 ttc Phe gaa Giu tgg Trp aag Lys aac Asn 240 aac Asn ggc Gly gaa Giu gt t Val ctg Leu c tg Leu tcc Ser atc Ile ctg Leu 145 gtg Val cgc Arg c ta Leu cag Gin gaa Giu 225 cca Pro gtt Val1 tcc Ser gat Asp gca Ala att Ile gt t Val cag Gin cct Pro 130 att Ile c ac His a tg Met atc Ile t ac Tyr 210 gtt Val atg Met tac Tyr t ca Ser att Ile cct Pro ga t Asp cag Gin act Thr 115 gac Asp cgc Arg t tc Phe gac Asp aag Lys 195 tcc Ser gtg Val atc Ile gca Ala PCTIiBOO/00923 atg cca 211 Met Pro tct ctg 259 Ser Leu cag tgg 307 Gin Trp, ccg atg 355 Pro Met atg ggc 403 Met Gly 100 gat ttt 451 Asp Phe gat gtc 499 Asp Val cgt act 547 Arg Thr tac aac 595 Tyr Asn 165 aag gtg 643 Lys Val 180 acc atc 691 Thr Ile cct gag 739 Pro Giu gac gca 787 Asp Ala atc aac 835 Ilie Asn 245 gac tcc 883 Asp Ser 260 att gaa tgg atg cac cgc aat cta aac cgt cgt gat tcc att atc ctg WO 01/00843 Ile Glu Trp tcc ctg cac Ser Leu His 280 PCTIiBOO/00923 His Arg Asn Leu Arg Arg Asp cac aat gac His Asn Asp ctg ggc 1027 Leu Gly 295 aac ggc 1075 Asn Gly 310 atg ctg 1123 Met Leu cag atc 1171 Gin Ile cgc cac 1219 Arg His cac cag 1267 His Gin 375 cag cca 1315 Gin Pro 390 acc gaa 1333 Thr Giu tac Tyr atg gct ggc gct Met Ala Gly Ala 300 ggc acc ggc gtt Gly Thr Gly Val cgc atc gaa ggc Arg Ile Glu Gly 305 tgc ctg gtc acc Cys Leu Val Thr Ser Ile Ile Leu 275 ggc gca gct gag 979 Gly Ala Ala Giu 290 tgc ctg ttc ggc Cys Leu Phe Gly ctg gca ctg aac Leu Ala Leu Asn 325 acc gat ata cgc Thr Asp Ile Arg gag cgc acc ggc Giu Arg Thr Gly 315 acc cag ggc gtt Thr Gin Gly Val 330 cgc agc acc gtt Ara Ser Thr Val aac Asn gtc Val1 320 gac cct cag ctg Asp Pro Gin Leu 335 gac Asp t tc Phe 340 gaa tac Giu Tyr 345 cca tac Pro Tyr tgc aac cag ctg cgc gtt cct gag Cys Asn Gin Leu Arg Val Pro Giu 350 355 gtc ttc acc gct ttc tcc ggt tcc Vai Phe Thr Ala Phe Ser Giy Ser 370 ggc ggt gac ctg Gly Giy Asp Leu 365 gct gtg aac aag Ala Val Asn Lys 380 gct agc tcc act Ala Ser Ser Thr ggt ctg gac gcc atg gct gcc aag gtt Gly Leu Asp Ala Met Ala Ala Lys Val 385 gaa gtt tct tgg gag cag ctg cgc gac Giu Val Ser Trp Glu Gin Leu Arg Asp 400 405 gg t Gly 395 tgg gag gtt cct Trp Giu Val Pro 410 <210> 276 <211> 411 <212> PRT <213> Corynebacteriun giutanicun <400> 276 Met Ser Pro Asn Asp Ala Phe Ile Ser Ala Pro Ala Lys Ile Giu Thr 1 5 10 Pro Val Gly Pro Arg ASn Giu Gly Gin Pro Ala Trp Asn Lys Gin Arg 25 Gly Ser Ser Met Pro Val Asn Arg Tyr Met Pro Phe Giu Vai Giu Val WO 01/00843 WO 0100843PCTIIBOO/00923 Giu Val1 Leu Leu Ser Ile Leu 145 Val Arg Leu Gin Giu 225 Pro Val Asp Val Gly 305 Thr Phe Leu Asp Aia Ile Val Gin Pro 130 Ile His Met Ile Tyr 210 Val1 Met Tyr Ser Gly 290 Cys Leu Thr Arg Ile Pro Asp Gin Thr 115 Asp Arg Phe Asp Lys 195 Ser Val Ile Ala Ile 275 Ala Leu Ala Asp Val 355 Ser Gin Pro Met 100 Asp Asp Arg Tyr Lys 180 Thr Pro Asp Ile Asp 260 Ile Ala Phe Leu Ile 340 Pro Leu Trp, Met Gly Phe Val Thr Asn 165 Vai Ile Giu Ala Asn 245 Ser Leu Giu Gly Asn 325 Arg Glu Pro Cys 70 Ser Phe Asp Thr Phe 150 Ser Gin Ala Ser Val1 230 Leu Ile Ser Leu Asn 310 Met Gin Arg Trp Leu Lys Giu Giu Leu Giu Leu 170 Leu Pro Thr Met Val 250 Hius His Ala Thr Gly 330 Thr Gly Pro Arg Arg Val Ile Val Gly 155 Gin Ala Asp Giu Asp 235 Giu Arg Asn Gly Gly 315 Val Val Giy Asp Asp Arg Gly Ile Gin 140 Ala Arg Thr Thr Val1 220 Pro Met Asn Asp Ala 300 Asn Asp Giu Asp Lys Gly Met Phe Giu 125 Ala Lys Asn Asp Asn 205 Giu Thr Ile Leu Arg 285 Asp Val Pro Tyr Leu 365 WO 01/00843 WO 0100843PCTA~BOO/00923 Ala Phe Ser Gly Ser His Gin Asp Ala Val Asn Lys 370 375 380 Met Ala Ala Lys Val Gin Pro Gly Ala Ser Ser Thr 385 390 395 Glu Gin Leu Arg Asp Thr Glu Trp Giu Val Pro 405 410 Gly Leu Asp Ala Giu Val Ser Trp 400 <210> 277 <211> 714 <212> DNA <213> Corynebacteriun glutamicumr <220> <221> CDS <222> (101)..(691) <223> RXN02965 <400> 277 caggaggccg cacccacctg gtatccccag cagtcgcagc cgccaccgaa tccgcggacc ctgtcctcac ctgcagatat ctaaggaagg ctagaaaaca atg gaa aaa ttt Met Glu Lys Phe acc tac acc ggc Thr Tyr Thr Gly ggc gtt cca ctg cag cga tcc aac gtg Gly Val Pro Leu Gln Arg Ser Asn Val gac acc Asp Thr gac cag atc Asp Gin Ilie ttc gaa gac Phe Giu Asp cca gcc gtc tac Pro Ala Val Tyr C tC Leu aag cgc gtc acc Lys Arg Val Thr cgg acc ggc Arg Thr Gly ccc aac ttt Pro Asn Phe gga ctg ttt tcc Gly Leu Phe Ser tgg cgc caa aac Trp Arg Gln Asn gtc ctc Val Leu aac acc gac acc Asn Thr Asp Thr aag aac ggc tcc Lys Asn Gly Ser ctc gta gca ggc Leu Val Ala Gly 307 355 cct Pro gac ttt ggc acc Asp Phe Gly Thr tcc tcc cgc gag Ser Ser Arg Giu gcc gtc tgg gca Ala Val Trp Ala atg gac tac ggc Met Asp Tyr Gly cgc gct gtc ttc tcc tca cga ttc gcc Arg Ala Val Phe Ser Ser Arg Phe Ala gac atc Asp Ile 100 ttc cgc ggc Phe Arg Gly cag tcc gac Gin Ser Asp 120 tcc gga aaa gcg Ser Gly Lys Ala ggC Gly 110 atg ctc acc ggc Met Leu Thr Gly atc atg gaa Ile Met Giu 115 acc cca ggc Thr Pro Gly 451 499 atc gaa ctt ctg Ile Glu Leu Leu aag ctc atg gaa Lys Leu Met Glu caa Gin 130 ctc gaa ctg acc gtg aac ctg gaa aag cag atc gtc acc gca ggc gac WO 01/00843 Leu Glu Leu Thr Val Asn Leu Glu Lys 135 140 gta gtg atc agc ttc gaa gtt gac ccc Val Val Ile Ser Phe Glu Val Asp Pro 150 155 gaa ggc ctc gac gac gct ggc ctg acc Giu Gly Leu Asp Asp Ala Giy Leu Thr 170 gaa gac tac gag gct aag cgc cct gcg Glu Asp Tyr Giu Ala Lys Arg Pro Ala 185 190 taagtttcag tctgatagcg aaa <210> 278 <211> 197 <212> PRT <213> Corynebacterium glutamicum <400> 278 Met Glu Lys Phe Thr Thr Tyr Thr Gly Gin Ile Val Thr 145 tac atc cgc tgg Tyr Ile Arg Trp 160 ctg cgc aag ctc Leu Arg Lys Leu 175 ttt *aag cca cgc Phe Lys Pro Arg PCTIBOO/00923 Ala Gly Asp cgt ttg atg 595 Arg Leu Met 165 gat gaa att 643 Asp Giu Ile 180 act aac gct 691 Thr Asn Ala 195 1 Ser Val Asn Val Ala Arg Thr Glu Val 145 Arg Lys Asn Thr Asp Leu Val Phe Gly Gin 130 Thr Trp Leu Val Arg Pro Vai Trp Al a Ile 115 Thr Ala Arg Asp Asp Thr Asn Ala Ala Asp 100 Met Pro Gly Leu Giu 180 Thr Gly Phe G ly Leu Ile Glu Gly Asp Met 165 Ile Gin Giu Leu 55 Asp Asp Arg Ser Giu 135 Val1 Giy Asp Ile Asp 40 Asn Phe Tyr Gly Asp 120 Leu Ile Leu Tyr Ile 25 Giy Thr Giy Giy Asn 105 Ile Thr Ser Asp Glu 185 Gly Ala Phe Thr Gly Arg Gly Leu Asn Glu 155 Ala Lys Val Vali Ser Tyr Ser Aia Lys Leu Leu 140 Vai Giy Arg Pro Tyr Asn Lys Ser Val Ala Trp, 125 Glu Asp Leu Pro Leu Leu Trp Asn Arg Phe Gly 110 Lys Lys Pro Thr Ala 190 Pro Arg Thr Asn Aia WO 01/00843 WQ 0100843PCT/iBOOIO0923 195 <210> 279 <211> 936 <212> DNA <213> Corynebacterium giu tamicun <220> <221> <222> <223>

CDS

(101)..(913) RXN0 1929 <400> 279 aaaatttgac tccataacga gaacttaatc gagcaacacc cctgaacagt gaatcaaatc ggaatttatt tattctgagc tggtcatcac atctatactc atg ccc atg tca ggc Met Pro Met Ser Gly att gat gca aag Ile Asp Ala Lys atc cgc acc cgt cat ttc cgc gaa gct Ile Arg Thr Arg H-is Phe Arg Giu Ala aaa gta Lys Val aac ggc cag Asn Gly Gin gtt tcg gtt ctc Val Ser Val Leu agc tat gat gcg Ser Tyr Asp Ala ctt tcg gcg Leu Ser Ala ggt gat tcc Giy Asp Ser cgc att ttt gat gag gct ggc Arg Ile Phe Asp Glu Ala Gly gat atg ctc ctt Asp Met Leu Leu gct gcC Ala Ala aac gtt gtg ctg Asn Val Val Leu gg t Gly cgc gat acc acc Arg Asp Thr Thr tcg atc acc ttg Ser Ile Thr Leu gat Asp gag atg att gtg Giu met Ile Val c tg Leu gcc aag gcg gtg Ala Lys Ala Val atc gct acg aag Ile Ala Thr Lys gcg ctt gtg gtg Ala Leu Val Val gat ctg ccg ttt Asp Leu Pro Phe acc tat gag gtg Thr Tyr Giu Val agc cca Ser Pro 100 aat cag gcg Asn Gin Ala gct gcg gtg Ala Ala Val 120 gag tcc gcg atc cgg gtc atg cgt gaa Giu Ser Ala Ile Arg Val Met Arg Giu 110 acg ggt gcg Thr Gly Ala 115 acg att cga Thr.Iie Arg aag atc gag ggt Lys Ile Giu Gly gtg gag atc gcg Val Giu Ile Ala cgc att Arg Ile 135 gtt gat gct gga Val Asp Ala Gly att Ile 140 ccg gtt gtc ggC Pro Val Val Gly atc ggg tac acc Ile Giy Tyr Thr ccg cag tcc gag cat Pro Gin Ser Giu His tcC Ser 155 ttg ggc ggc cac gtg gtt cag ggt cgt Leu Gly Gly His Val Val Gin Gly Arg gcg agt tct gga aag ctc atc gcc gat gcc cgc gcg ttg gag cag gcg WO 01/00843 Ala Ser Ser ggt gcg ttt Gly Ala Phe gag gtt acc Giu Val Thr 200 aat ggc aca Asn Gly Thr 215 aac cgc ggc Asn Arg Gly 230 gat tcc ttg Asp Ser Leu ggt acc ttc Gly Thr Phe Gly gcg Ala 185 gag Giu ga t Asp aag Lys cac His cca Pro 265 PCTIBOO/00923 Arg Ala Leu Glu Gin Ala 180 cca gca gag gca gcg cgc 691 Pro Ala Glu Ala Ala Arg 195 atc gga atc ggt gcc ggc 739 Ile Gly Ile Gly Ala Gly 210 cag gat gcc ttc ggc ctc 787 Gin Asp Ala Phe Gly Leu 225 gag tac gcc acc ttg ggc 835 Giu Tyr Ala Thr Leu Gly 240 245 atc gcc gat atc cac gcg 883 Ile Ala Asp Ile His Ala 260 taatgcaggt agcaaccaca 933 936 aag <210> 280 <211> 271 <212> PRT <213> Corynebacterium giutamicum <400> 280 Met Pro Met Ser Gly Ile Asp Ala Lys 1 5 Arg Glu Ala Lys Val Asn Gly Gln Lys' 25 Asp Ala Leu Ser Ala Arg Ile Phe Asp 40 Leu Val Gly Asp Ser Ala Ala Asn Val' Leu Ser Ilie Thr Leu Asp Giu Met Ile' 70 Ile Ala Thr Lys Arg Ala Leu Val Val' Tyr Giu Val Ser Pro.Asn Gin Ala ValI 100 105 Arg Giu Thr Gly Ala Ala Ala Val Lys 115 120 Ala Gin Thr Ilie Arg Arg Ile Val Asp, 411 Ile Ser Ala Leu Leu Asp Ser Giu Giy Thr Leu Val1 Arg Lys Pro Ile Giy 125 Pro Arg Thr Asp Asp Ala Phe Arg 110 Val1 Val1 His Ser Met Thr Val Gly Val Giu Val WO 01/00843 130 His Ile GI 145 Val Gin GI Ala Leu GI Ala Giu Al Gly Ile GI 210 Asp Aia P1 225 Tyr Ala T1 Ala Asp I] PCT/1-BOO/00923 Thr Gly 165 Ala Arg Giy Leu Gly 245 Ala Pro 150 Ala Giy Glu Asn Asn 230 Asp Gly 135 Gin Ser Ala Vai Gly 215 Arg Ser Thr Glu Giy Ala 185 Glu Asp Lys His Pro 265 His Ala 175 Val Thr Trp Arg Tyr 255 Phe Val1 160 Arg Pro Ile Gin Giu 240 Ile <210> 281 <211> 930 <212> DNA <213> Corynebacterium giutanicum <22 0> <221> CDS <222> (101)..(907) <223> FRXA01929 <400> 281 tgactccata acgagaactt aatcgagcaa cacccctgaa tatttattct gagctggtca tcacatctat actcatgccc gca aag Ala Lys cag aaa Gin Lys ttt gat Phe Asp aac gtt Asn Val atg att acc Thr ctc Leu gtc Val cgc Arg aag cg t Arg ac c Thr gat Asp ga t Asp 60 gcg cat ttc cgc gaa His Phe Arg Glu agc tat gat gcg Ser Tyr Asp Ala atg ctc ctt gtt Met Leu Leu Val acc acc ttg tcg Thr Thr Leu Ser gtg acg atc gct 412 cagtgaatca aatcggaatt atg tca ggc att gat Met Ser Gly Ile Asp 1 gct aaa gta aac ggc Ala Lys Val Asn Gly ctt tcg gcg cgC att Leu Ser Ala Arg Ile ggt gat tcc gct gcc Gly Asp Ser Ala Ala atc acc ttg gat gag Ile Thr Leu Asp Giu acg aag cgt gcg ctt 115 163 211 259 307 355 WO 01/00843 Met Ilie Val gtg gtg gtt Val Val Val gcg gtg gag Ala Val Giu gtg aag atc Val.Lys Ile 120 gtt gat gct Val Asp Ala 135 tcc gag cat Ser Giu His 150 tct gga aag Ser Gly Lys ttt gcg gtt Phe Ala Val acc gag gat Thr Giu Asp 200 aca gat ggg Thr Asp Gly 215 ggc aag aag Gly Lys Lys 230 ttg cac gac Leu His Asp Leu gat Asp t cc Ser 105 gag Giu gga Gly tcc Ser ctc Leu gtg Val 185 ctt Leu cag Gin cca Pro gcc Ala Ala LYS 75 ctg ccg Leu Pro gcg atc Ala Ile ggt ggc Gly Gly att ccg Ile Pro ttg ggc Leu Giy 155 atc gcc Ile Ala 170 ttg gag Leu Glu tcc atc Ser Ile gtt ttg Val Leu cgc ttc Arg Phe 235 gcg cag Ala Gin 250 Val Thr Ile ggt acc tat Gly Thr Tyr 95 gtc atg cgt Val Met Arg 110 gag atc gcg Giu Ile Ala 125 gtc ggc cac Vai Giy His cac gtg gtt His Vai Val gcc cgc gcg Ala Arg Ala 175 gtt cca gca Vai Pro Ala 190 act atc gga Thr Ilie Gly 205 tgg cag gat Trp Gin Asp cgc gag tac Arg Giu Tyr tac atc gcc Tyr Ile Ala 255 Ala 80 gag Giu gaa Giu cag Gin atc Ile cag Gin 160 t tg Leu gag Giu atc Ile gcc Ala gcc Ala 240 ga t Thr gtg Val acg Thr acg Thr ggg Gly 145 gg t G ly gag Glu gca Ala gg t Gly ttc Phe 225 ac c Thr atc PCTIBOO/00923 Ala Leu aat cag 403 Asn Gin 100 gct gcg 451 Ala Ala cgc att 499 Arg Ile ccg cag 547 Pro Gin gcg agt 595 Ala Ser 165 ggt gcg 643 Gly Ala 180 gag gtt 691 Giu Val aat ggc 739 Asn Gly aac cgc 787 Asn Arg gat tcc 835 Asp Ser 245 ggt acc 883 Gly Thr 260 Asp Ile His Ala ttc cca ggc gaa gcg gag tcc ttt taatgcaggt agcaaccaca aag Phe Pro Giy-Giu Ala 265 Giu Ser Phe <210> 282 <211> 269 <212> PRT <213> Corynebacteriun giutanicun <400> 282 Met Ser Gly Ile Asp Ala Lys Lys Ilie Arg Thr Arg His Phe Arg Glu 1 5 10 WO 01/00843 Ala Lys Val Leu Ser Ala Gly Asp Ser Ile Thr Leu Thr Lys Arg Vai Ser Pro Thr Gly Ala 115 Thr Ile Arg 130 Gly Tyr Thr 145 Gly Arg Gly Giu Gin Ala Ala Ala Arg 195 Gly Ala Gly 210 Phe Gly Leu 225 Thr Leu Gly Ile His Ala Asn Arg Ala Asp Ala Asn 100 Ala Arg Pro Ala Gly 180 Giu Asn Asn Asp Gly 260 Gly Ile Ala Glu Leu Gin Al a Ile Gin Ser 165 Ala Val Gly Arg Ser 245 Thr Gin Phe Asn met 70 Val Ala Val1 Val1 Ser 150 Ser Phe Thr Thr Gly 230 Leu Phe Val Glu 40 Val Val Val Giu Ile 120 Ala His Lys Val1 Asp 200 Gly Lys Asp Gly Ser Ala Leu Leu Asp Ser 105 Glu Gly Ser Leu Val 185 Leu Gin Pro Ala Giu 265 Val1 Giy Gly Ala Leu 90 Ala Gly Ile Leu Ile 170 Leu Ser Val Arg Ala 250 Ala Leu Val Arg Lys 75 Pro Ile Gly Pro Giy 155 Ala Giu Ile Leu Phe 235 Gin Glu Thr Asp Asp Ala Phe Arg Val Vai 140 Gly Asp Met Thr Val 220 Val1 Ala Ser Ser Met Thr Val1 Gly Val1 Giu 125 Vai His Ala Val Thr 205 Trp Arg Tyr Phe PCTIIBOO/00923 Asp Ala Leu Val Leu Ser Ile Ala Tyr Giu Arg Glu Ala Gin His Ile Val Gin 160 Ala Leu 175 Ala Glu Gly Ile Asp Ala Tyr Ala 240 Ala Asp 255 <210> 283 <211> 1065 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS <222> (101)..(1042) <223> RXN01420 <400> 283 cgaagttcac gactacaggc attacctggg tgctgatttt gcgcgagtaa agctgtggct 414 WO 01/00843 WO 0100843PCTIBOO/00923 gcggaatgcg ctgctcatcc acacgtggaa tcctgattgg gtg acg ctg ggt gga Val Thr Leu Gly Gly ttg aat gta cca Leu Asn Val Pro tgg tcg ctg ggc Trp Ser Leu Gly gaa atg ctg ttc Glu Met Leu Phe tac ctg Tyr Leu acg ttc ccg Thr Phe Pro aac tgg tgg Asn Trp Trp, ttt att ccg tta gtg cgc aaa gtg aag Phe Ilie Pro Leu Val Arg Lys Val Lys ggc gtg ggc Gly Val Gly gcg ctg att Ala Leu Ile gca ttt ggc atc Ala Phe Gly Ile ttt gcc gtg agc Phe Ala Val Ser aca gtg att cac ttt tat Thr Val Ile His Phe Tyr gcg gac gga cca aag Ala Asp Gly Pro LYS att gag aac ttc Ile GiU Asn Phe gtt cct cgc ctg Val Pro Arg Leu tgg Trp gac acc aat gtg Asp Thr Asn Val ccg gtc gcg gaa Pro Val Ala Giu cac gcc gat cca His Ala Asp Pro tgg ttt atg cag Trp Phe Met Gin gaa att ccc gtg Giu Ile Pro Val ctg gaa Leu Giu 100 tct tac tgg Ser Tyr Trp tac ctc ggt Tyr Leu Giy 120 tct tac tac ttc ccg ctg acc aga ctc Ser Tyr Tyr Phe Pro Leu Thr Arg Leu 110 atc gag ttc Ile Giu Phe 115 atg ttt aaa Met Phe Lys gtg ttt ggc gcg Val Phe Gly Ala ctg gtt gct gaa Leu Val Ala Giu aac acc aac atc acc atc Asn Thr Asn Ile Thr Ile 135 ctg atc gca ctg gct gtt tct ttt gtt Leu Ile Ala Leu Ala Val Ser Phe Val 145 gc t Ala 150 act tgg ttt gtg Thr Trp Phe Val cca Pro 155 ctg gca ttc aag Leu Ala Phe Lys atg Met 160 tct gtc atc atg Ser Val Ile Met ctg cca atg gct Leu Pro Met Ala gtt gtg gca acg ctt gcg gtg aga gac Val Val Ala Thr Leu Ala Val Arg Asp 175 att gaa Ile Giu 180 ggc aag agt Gly Lys Ser att tcc ttt Ile Ser Phe 200 gaa atc gcc tcg cct cgc gca gtt ttg Glu Ile Ala Ser Pro Arg Ala Val Leu 190 ttg ggt aat Leu Gly Asn 195 gtg ttt gtg Val Phe Val gcc ttc tac atg Ala Phe Tyr Met caa ttc ccc gtc Gin Phe Pro Val cag cgc Gin Arg 215 tat ttc att gct Tyr Phe Ilie Ala aaa gaa tac ggc Lys Giu Tyr Gly t tc Phe 225 ctt ggc tgg gca Leu dly Trp Ala 787 WO 01/00843 ttc tat gca gtg gtg tgt ttc atc gtg tcg gtc att Phe Tyr Ala Vai Val Cys Phe Ile Val Ser Val Ilie 230 235 240 ctg ttt acc ttc gtt gat gat cct ttg atg aag gcc Leu Phe Thr Phe Val Asp Asp Pro Leu Met Lys Ala 250 255 aag ggg agt agg cgc ttg aag cag tcc aat att ttg Lys Gly Ser Arg Arg Leu Lys Gin Ser Asn Ile Leu 265 270 aaa gtt ctc ttc ggc aaa agc ccc gaa aag ccg tta Lys Val Leu Phe Gly Lys Ser Pro Glu Lys Pro Leu 280 285 cgc gct gag aat ctc aca gaa aac tcc gaa gct ccc 1027 Arg Ala Giu Asn Leu Thr Glu Asn Ser Giu Ala Pro 295 300 305 acc gga atc aaa tct tagggaagga aaacatatgg cta 1065 Thr Gly Ile Lys Ser 310 <210> 284 <211> 314 <212> PRT <213> Corynebacteriun glutaiicun PCT/1B00100923 tgg gtg 835 Trp, Val 245 cgc aag 883 Arg Lys 260 gac ctg 931 Asp Leu gag act 979 Glu Thr gta gct Val Ala <400> 284 Val Thr Leu Giy Leu Asn Val Pro 1 Met Val Ser Gly Pro Ile Arg Giu Ala Phe Gly Ala Glu Ala Val Ile 115 Met Ser Phe Trp, Val Val Ala Leu Thr 135 Thr Pro Trp Ile Pro Asp Trp Gly 120 Asn Trp, Leu Ala His Arg Pro Leu 105 Val Ile Phe Ser Phe Phe Phe Leu Val Ser Phe Thr Val Trp Ile Gly Tyr Trp 75 Trp Tyr Gly Ile Pro Glu Lys Val Lys Ser Giu Thr Ala Leu Met WO 01/00843 145 150 155 Ser Val Ilie met Ser Leu Pro Met Ala Phe Vai 165 170 Val Arg Asp Ile Giu Gly Lys Ser Gly Giu Ile 180 185 Vai Leu Leu Gly Asn Ilie Ser Phe Ala Phe Tyr 195 200 Val Met Val Phe Val Gin Arg Tyr Phe Ile Ala 210 215 Phe Leu Giy Trp Ala Phe Tyr Ala Val Val Cys 225 230 235 Ile Leu Ala Trp Val Leu Phe Thr Phe Val Asp 245 250 Ala Thr Ala Arg Lys Lys Gly Ser Arg Arg Leu 260 265 Leu Val Arg Asp Leu Lys Val Leu Phe Gly Lys 275 280 Leu Lys Val Giu Thr Arg Ala Giu Asn Leu Thr 290 295 Pro Aia Lys Vai Ala Thr Giy Ile Lys Ser 305 310 <210> 285 <211> 1137 <212> DNA <213> Corynebacterium glutainicum <220> <221> CDS <222> (101)..(1114) <223> RXS01145 <400> 285 taatgtagtt gtctgcccaa gcgagttaaa ctcccacgat cttttcacca aaatttttac gaaaggcgag attttctccc ctt tat gat gct gac gct gac ctc tcc ttg atc Leu Tyr Asp Ala Asp Ala Asp Leu Ser Leu Ile 15 gcc atc gtt ggc tac ggc tcc cag ggc cac gca Ala Ilie Val Gly Tyr Gly Ser Gin Gly His Ala 30 cgc gat tct ggc gtt gag gtt gtc att ggt ctg Arg Asp Ser Gly Val Glu Val Val Ile Gly Leu 45 Ala Ser Val1 205 Lys Ile Pro Gin Pro 285 Asn Thr Pro 190 Gin Giu Val Leu Ser 270 Giu Ser PCTIIBOO/00923 160 Leu Ala 175 Ax-g Ala Phe Pro Tyr Gly Ser Val 240 Met Lys 255 Asn Ile Lys Pro Giu Ala ttacagtggg gggcagacat atg gct att gaa ctg Met Ala Ile Giu Leu 1 cag ggc cgt aag gtt Gin Gly Arg Lys Val cac tcc cag aac ctc His Ser Gin Asn Leu cgc gag ggc tcc aag Arg Giu Gly Ser Lys WO 01/00843 WO 0100843PCTIIBOO/00923 tcc gca Ser Ala gag aag gca aag Glu Lys Ala Lys gca ggc ttc gag Ala Gly Phe Glu aag acc acc gct Lys Thr Thr Ala gag Glu gct gca gct tgg Ala Ala Ala Trp gac gtc atc atg Asp Val Ile Met ctg gct cca gac Leu Ala Pro Asp 307 355 403 tcc cag gca gaa Ser Gln Ala Glu ttc acc aac gac Phe Thr Asn Asp gag cca aac ctg Glu Pro Asn Leu aac gca Asn Ala 100 ggc gac gca Gly Asp Ala atc aag cca Ilie Lys Pro 120 ctg ttc ggc cac Leu Phe Gly His ctg aac att cac Leu Asn Ile His ttc gac ctg Phe Asp Leu 115 cca aag ggc Pro Lys Gly gct gac gac atc atc gtt ggc atg gtt Ala Asp Asp Ile Ile Val Gly met Val cca ggc Pro Gly 135 cac ttg gtt cgc His Leu Val Arg cgt Arg 140 cag ttc gtt gat Gln Phe Val Asp aag ggt gtt cct Lys Gly Val Pro tgc Cys 150 ctc atc gca gtc Leu Ile Ala Val gac Asp 155 cag gac cca acc Gln Asp Pro Thr acc gca cag gct Thr Ala Gln Ala c tg Leu 165 acc ctg tcc tac Thr Leu Ser Tyr gca Ala 170 gca gca atc ggt Ala Ala Ile Giy gca cgc gca ggc Ala Arg Ala Gly gtt atc Val Ile 180 cca acc acc Pro Thr Thr gct gtt ctc Ala Val Leu 200 gaa gct gag acc Glu Ala Glu Thr acc gac ctc ttc Thr Asp Leu Phe ggc gag cag Gly Giu Gln 195 ggc ttc gag Gly Phe Glu tgc ggt ggc acc Cys Gly Giy Thr gaa ctg gtc aag Glu Leu Val Lys gtt ctc Vai Leu 215 acc gaa gct ggc Thr Glu Ala Giy gag cca gag atg Glu Pro Giu Met tac ttc gag gtt Tyr Phe Glu Val cac gag ctc aag His Giu Leu Lys c tc Leu 235 atc gtt gac ctc Ile Vai Asp Leu ttc gaa ggt ggc Phe Glu Gly Gly atc Ile 245 agc aac atg aac Ser Asn Met Asn tac Tyr 250 tct gtt tct gac Ser Val Ser Asp gct gag ttc ggt Ala Glu Phe Gly ggc tac Gly Tyr 260 ctc tcc ggc cca cgc gtc atc gat Leu Ser Gly Pro Arg Val Ile Asp 265 gac acc aag tcc Asp Thr Lys Ser cgc atg aag Arg Met Lys 275 cgc ctc atc Arg Leu Ile gac atc ctg Asp Ile Leu 280 acc gat atc cag Thr Asp Ile Gin ggc acc ttc acc Gly Thr Phe Thr aag Lys 290 WO 01/00843 gca aac gt 1027 Ala Asn Vi 295 tac aac aE 1075 Tyr Asn A~c 310 atg agc t5 1124 Met Ser Ti ggc aac acc gag Gly Asn Thr Giu 300 atc gag gag acc Ile Giu Glu Thr 315 gtt gac gct cgc Val Asp Ala Arg PCTJLB00100923 ctt gag ggc ctt cgt gct tcc Leu Giu Gly Leu Arg Ala Ser 305 ggc gct aag ctc cgc gac ctc Gly Ala Lys Leu Arg Asp Leu 320 325 gca gaa acc gct taagtttcac Ala Giu Thr Ala 335 ;g gtc aag Val Lys 330 ccctttgacg gct 1137 <210> 286 <211> 338 <212> PRT <213> Corynebacterium giutamicum <400> 286 Met Ala Ile Glu Leu Leu Tyr Asp Ala 1 Gin His Arg Val Leu Pro Ile Val Gly 145 Thr Arg Ile Asp Ala Ala Gin Asp Lys G ly 135 Leu Leu Thr Val Ser Giu Ala Ala Ala Pro 120 His Ile Ser Thr Gly Gly Lys Ala Glu Leu 105 Ala Leu Ala Tyr Phe 185 Asp .10 Tyr Val Ala Trp Ile Leu Asp Val Val Ala 170 Glu Asp Ser Val Giu Asp Thr Gly Ile Arg 140 Gin Ala Glu Leu Gin Val Ala Val Asn His Ile 125 Gin Asp Ile Thr Leu His Gly Phe Met Ile Leu Gly Val Thr Gly 175 Thr WO 01/00843 Leu Phe GI is Lys Val G) 210 Ala Tyr P1 225 Phe Glu G3 Glu Phe G) Lys Ser Ai Thr Lys Ai 290 Gly Leu Ai 305 Lys Leu Ai Thr Ala Gin Giu Val1 Ile 245 Tyr Lys Ile Ser Leu 325 Vai Leu 215 His Asn Ser Ile Asn 295 Asn Ser Leu 200 Thr Giu Met Giy Leu 280 Vai Asn Trp Cys Giu Leu Asn -Pro 265 Thr Glu His Val Gly Ala Lys Tyr 250 Arg Asp Asn Pro Lys 330 Giy Gly Leu 235 Ser Val1 Ile Gly Ile 315 Val PCTIBOO/00923 Leu Val Glu Met Leu Met 240 Thr Aia 255 Asp Thr Thr Phe Leu Glu Gly Ala 320 Ala Glu 335 Asp Ala Arg <210> 287 <211> 556 <212> DNA <213> Corynebacterium giutanicum <220> <221> ODS <222> (101)..(556) <223> FRXA01145 <400> 287 taatgtagtt gtctgcccaa gcgagttaaa ctcccacgat cttttcacca aaatttttac gaaaggcgag attttctccc ctt Leu gcc Ala cgc Arg tcc tat Tyr a tc Ile ga t Asp gca gat

ASP

gtt Val1 tc t Ser gag gct Ala ggc Giy ggC G ly tcc ttg Ser Leu ggc cac Gly His 30 att ggt Ile Gly ggc ttc ttacagtggg gggcagacat atg gct att gaa ctg Met Ala Ile Giu Leu 1 cag ggc cgt aag gtt Gln Gly Arg Lys Val cac tcc cag aac ctc His Ser Gin Asn Leu cgc gag ggc tcc aag Arg Glu Giy Ser Lys gtc aag acc acc gct aag gca aag gaa WO 01/00843 Ser Ala Giu Lys Ala Lys Giu Ala Gly 60 gag gct gca gct tgg gct gac gtc atc Giu Ala Ala Ala Trp Ala Asp Val Ile 75 tcc cag gca gaa atc ttc acc aac gac Ser Gin Ala Giu Ile Phe Thr Asn Asp ggc gac gca ctg ctg ttc ggc cac ggc Gly Asp Ala Leu Leu Phe Gly His Giy 105 110 atc aag cca gct gac gac atc atc gtt Ilie Lys Pro Ala Asp Asp Ile Ilie Val 120 125 cca ggc cac ttg gtt cgc cgt cag ttc Pro Gly His Leu Val Arg Arg Gin Phe 135 140 tgc ctc atc Cys Leu Ile 150 <210> 288 <211> 152 <212> PRT <213> Corynebacterium giutainicum <400> 288 Met Ala Ile Giu Leu Leu Tyr Asp Ala 1 Gin Gly Arg Lys Val Ala Ile Val Gly His Ser Gin Asn Leu Arg Asp Ser Gly Arg Giu Gly Ser Lys Ser Ala Giu Lys 55 Val Lys Thr Thr Ala Giu Ala Ala Ala Leu Ala Pro Asp Thr Ser Gin Ala Giu Pro Asn Leu Asn Ala Gly Asp Ala Leu 100 105 Ilie His Phe Asp Leu Ile Lys Pro Ala 115 120 Val Ala Pro Lys Gly Pro Gly His Leu 130 135 Giu ctc Leu 80 gag Giu aac Asn atg Met gat Asp Val c tg Leu cca Pro att Ile gtt Val ggc Gly 145 Lys Thr gct cca Ala Pro aac ctg Asn Leu cac ttc His Phe 115 gcg cca Ala Pro 130 aag ggt Lys Gly PCTIIBOO/00923 Thr Ala gac acc 355 Asp Thr aac gca 403 Asn Ala 100 gac ctg 451 Asp Leu aag ggc 499 Lys Gly gtt cct 547 Val Pro Asp Ser Val Giu Asp Thr Gly Ile Arg 140 WO 01/00843 WO 0100843PCTIBOOIOO923 Gly Lys Gly Val Pro Cys Leu Ile 145 150 <210> 289 <211> 1350 <212> DNA <213> Corynebacterium giutaniicum <220> <221> CDS <222> (101)..(1327) <223> RXA02375 <400> 289 cttttcaggt ctacgtgtat acgatggtaa cgctatgaat gatacgcaga acacacctga aagcgttcga ttacgggata atctcccaac gccaacccaa atg gcg ccg gtg aca Met Ala Pro Val Thr ggg ctt cct gtc Giy Leu Pro Val ccc tac agc cag Pro Tyr Ser Gin gca agc atc ggt Ala Ser Ile Gly gcg agc Ala Ser ttc ccg gca Phe Pro Ala gaa tcc gga Giu Ser Gly gtg Val gat ccg gac acc Asp Pro Asp Thr gac agc gcc gca Asp Ser Ala Ala tac gga cat Tyr Gly His gtg gtg gtg Val Val Val atg cgt gag cgc atc tcc aac gct aag Met Arg Giu Arg Ile Ser Asn Ala Lys 45 cga Arg aaa att Lys Ile ggt tcg tcc tca ttg act aac gat gag Gly Ser Ser Ser Leu Thr Asn Asp Giu gga cac acc gtc Gly His Thr Val ga t

ASP

ccc aac cgc atc Pro Asn Arg Ile act att gtc aat Thr Ilie Val Asn ttg caa gca cgc Leu Gin Ala Arg gaa gct ggc tcg gac ctc atc gtt gtg Glu Ala Gly Ser Asp Leu Ile Val Val tct ggc gca gtg Ser Gly Ala Val gcc gcg Ala Ala 100 gga atg gcc Gly Met Ala aag cag gct Lys Gin Ala 120 ccg Pro 105 ctt gga ttg agc acc cgg ccc acg gaa Leu Gly Leu Ser Thr Arg Pro Thr Giu 110 ttg gca gtc Leu Ala Val 115 cac cag tgg His Gin Trp gca gca gca gtg Ala Ala Ala Val caa gtt cac ctc Gin Val His Leu a tg Met 130 gga cgt Gly Arg 135 tct ttt gcc cgg Ser Phe Ala Arg ggt cgc ccc atc Gly Arg Pro Ilie cag gtg ctt ctt Gin Val Leu Leu acc Thr 150 gca gct gat gca Ala Ala Asp Ala aag cgt gat cgt Lys Arg Asp Arg agg aat gcg cag Arg Asn Ala Gin WO 01/00843 acc atc Thr Ile aat gac Asri Asp ctt gct Leu Ala ctc agt Leu Ser 215 gcg aag Ala Lys 230 att gcc Ile Ala gtg tct Val Ser acc tct Thr Ser act gta 1027 Thr Val 295 gct ttg 1075 Ala Leu 310 gcg gtg 1123 Ala Val att act 1171 Ile Thr ttg gga 1219 Leu Gly tct gat 1267 Ser Asp 375 gac Asp acc Thr gca Ala 200 gac Asp ttt Phe ggc Gly gc t Ala gcg Ala 280 ttc Phe tat Tyr gaa Giu gaa Glu cc t Pro 360 acc Thr aag ctg cgc att ttg ggc gcg gtt cct atc gtc Lys Leu Arg Ile Leu Gly Ala Val Pro Ile Val 170 175 gca Ala gtg Val1 ga t Asp tcc Ser ggc Gly 250 cg t Arg aac Asn ccc Pro gca Ala gtg Val 330 att Ilie ggc Gly caa Gln gg t Gly ga t Asp cct Pro 225 gat Asp gg9c Gly g tg Val gac Asp gcg Al a 305 cga Arg ttg Leu gag Glu gag Glu acg Thr 385 gac Asp gc t Ala 210 act Thr ttg Leu a tg Met cct Pro gcc Ala 290 tgg Trp ctc Leu ctg Leu atc Ile gtg Val 370 cag Gin aac Asn 195 t tg Leu gat Asp aaa Lys gca Ala gtg Val 275 cag Gin aag Lys gat Asp gc t Ala gtg Val 355 tcc Ser gac Asp PCTIB00100923 aat gaa 643 Asn Giu 180 gac cga 691 Asp Arg gtg ctg 739 Val Leu ccc acc 787 Pro Thr ggt gtc 835 Gly Val 245 tca aag 883 Ser Lys 260 ctg, ttg 931 Leu Leu gtg ggC 979 Val Gly ttc tgg Phe Trp gac ggc Asp Gly 325 gtg ggc Val Gly 340 gag atc Giu Ile tac gat Tyr Asp ctt cca Leu Pro WO 01/00843 WO 0100843PCT/IBOO/00923 gat ggc atg cag cgc ccg gta gtg cat gca gat tat ctg tcc aac tac 1315 Asp Gly Met Gin Arg Pro Val Val H-is Ala Asp Tyr Leu Ser Asn Tyr 390 395 400 405 gcc agc cgc gcg taaagcgcgg gcctgctggt ggc 1350 Ala Ser Arg Ala <210> 290 <211> 409 <212> PRT <213> Corynebacterium glutainicum <400> 290 Met Ala Pro Val Thr Gly Leu Pro Val 1 Ser Ala Lys Asp Leu Gly Thr Leu Gly 145 Arg Pro Gly Asp Pro Ala Gly Gly Asn Gly Al a Ala 120 Ser Ala Asp Thr Ala 200 Asp Phe Val Met Ser Arg Ser Pro 105 Ala Phe Asp Lys Val 185 Ile Val Ile Thr 10 Asp Arg Ser Ile Asp Leu Ala Ala Ala Leu 170 Ala Va 1 Asp Ser Pro Pro Glu Ser Asn 75 Leu Gly Ala Arg Gly 155 Arg Thr Ala Gly Glu Ser Thr Ile Thr Ile Val Ser Gly 125 Gly Arg Leu Gly Leu 205 Phe Arg Glu Asp Asn Asp Asn Ser Arg Val Pro Arg Ala 175 Asn Ser Lys Gly Al a Ser Ala Glu Ala Ser Pro His Ile Ala 160 Val Phe Ala Asn Asn WO 01/00843 225 Asp Leu Ly Gly Met Al Val Pro Va 27 Asp Ala Gi 290 Ala Trp, Ly 305 Arg Leu As Leu Leu Al Glu Ilie Va Glu Val Se 370 Thr Gin As 385 Tyr Leu Se Ile Val Thr Thr Ala 310 Ala Ile Leu Ser Asp 390 Ala Aia Ser Ser Vai 295 Leu Val1 Thr Gly Asp 375 Gly Ser 235 Giy Leu Ile Lys Asp 315 Thr Giy Gin Ser Pro 395 Gly Arg 270 Ala Arg Gly Gly Gin 350 Gly Giy Hius PCTIBOOIOO923 240 Thr Giy 255 Ser Giy Leu Giu Leu Ser Lys Ile 320 Lys Ser 335 Gin Giy Arg Giy Met Gin Ala Asp 400 <210> 29i <211> 1419 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101). .(1396) <223> RXN02382 <400> 29i gagcagatgg ccaccgaggt cgatgtggtg gctggctact aggcctttta tggtgtgatc cgacactgtt gcttcttaag ggtctgtata gtgggcaacc atg agt tca acg acc 115 Met Ser Ser Thr Thr 1 cta act gat gac caa att cgc gac aat gag cgg acc gaa gtt cta gct 163 Leu Thr Asp Asp Gin Ile Arg Asp Asn Giu Arg Thr Glu Vai Leu Ala 15 aaa gca act gca gct aag aac atc gtc ccg gat att gca gtg ttg ggc 2ii Lys Ala Thr Ala Ala Lys Asri Ile Vai Pro Asp Ile Ala Vai Leu Giy 30 WO 01/00843 WO 0100843PCT/IB00100923 acc gga Thr Gly ccg aag aac gca atc ctg Pro Lys Asn Ala Ile Leu cgt gcg gcg gca gat gaa Arg Ala Ala Ala Asp Glu gca Ala ggt Gly ga t Asp ggc Gly aac Asn a tg Met gca Ala 150 gtg Val1 cgt Arg cgc Arg gtc Val gcg Ala 230 atc Ile ggc Gly cgc Arg cgc Arg gaa Glu c tg Leu ggc Gly gtc Val 135 c tc Leu cat H-is ttc Phe gga Gly a tc Ile 215 acc Thr ga t Asp aag Lys gca Ala aac Asn cgc Arg gac Asp 105 cag Gin gaa Giu tcc Ser aac Asn ctg Leu 185 gtc Val cgc Arg ccc Pro gaa Glu cgc Arg 265 gaa Giu ggc Gly att Ile cca Pro atg Met gcc Ala gga Giy acc Thr 170 cca Pro caa Gin ggc Gly acc Thr gcc Ala 250 cgc Arg atc I le 60 gaa Giu ggc Gly gg t Gly cag Gin cc t Pro 140 gta Val C tC Leu gaa Glu ttg Leu gca Ala 220 gaa Glu ctt Leu agc Ser gaa Glu gaa Glu a tc Ile gaa Glu gtc Val 125 aac Asn gc t Ala gtg Val acc Thr atc Ile 205 gga Gly acc Thr ga t Asp gtg Val gcc Ala tcc Ser gc t Ala gta Val1 110 cgt Arg gtc Val ttg Leu gaa Giu gtg Val1 190 acc Thr cta Leu ggc Gly cag Gin tgc Cys 270 aac Asn atg Met ggc Gly 95 ctg Leu gtg Val acc Thr c tg Leu atc Ile 175 cag Gin gca Ala atc Ile ac c Thr gca Al a 255 aac Asn gc t Ala att Ile 80 ggt Gly cgc Arg cc t Pro gtc Val1 cgc Arg 160 ctg Leu t tg Leu cgc Arg aac Asn ggc Gly 240 a tc Ile gc t Ala t cc Ser ga t Asp t tg Leu gga Gly t tg Leu gac Asp 145 ggt Gly cag Gin ctg Leu ggc Gly gca Ala 225 aac Asn gcc Ala act Thr ga t Asp cgc Arg cgc Arg cat His ggc Gly 130 gcc Ala t cc Ser gac Asp cct Pro ctc Leu 210 g tg Val tgc Cys atg Met gaa Glu c tc Leu gaa Glu gcc Ala gtt Val 100 atg Met a tg Met ggc G ly aca Thr c tc Leu 180 caa Gin gac Asp acc Thr ttc Phe atc Ile 260 gcg Ala gtt Val1 gcg Al a ctt Leu gc t Ala gaa Glu ggc Gly ctg Leu gc t Ala 165 gag Glu acc Thr gtg Val gg t Gly tac Tyr 245 aac Asn ctt Leu 259 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 ctc gac gcc gcc ctc agc gac tca gac aag ctt gca gtc gtc cag gcg WO 01/00843 Leu Asp Ala 280 PCTIiBOO/00923 Val Gin Ala ctc cag 1027 Leu Gin 295 gca ttc 1075 Ala Phe 310 tct gag 1123 Ser Glu gat gga 1171 Asp Gly gcg atc 1219 Ala Ile gtc gat 1267 Val Asp 375 ggg gag 1315 Gly Glu 390 ctg cat 1363 Leu His tgg att 1416 Trp Ile gaa Giu Ala Leu Ser Asp gca gga gtc aca Ala Giy Val Thr S300 cat gga cgg gtg gcc gaa ttg gaa His Gly Arg Val Ala Giu Leu Glu 305 gaa gca act gaa act gac tgg gat Giu Ala Thr Giu Thr Asp Trp Asp Asp Lys Leu Ala Vai 290 ggt gca acc Gly Ala Thr tac ctg tcc Tyr Leu Ser 330 gct ctg gca Ala Leu Ala gac Asp 315 g tg Val gtg Val1 320 ttc gat atc gct gtc Phe Asp Ilie Ala Val 335 cac atc gct aag tac His Ile Ala Lys Tyr 350 aac att gaa acc gct Asn Ile Glu Thr Ala gct gtg gtt gac ggt gtg Ala Val Val Asp Gly Val 340 agc acc aag cac acc gaa Ser Thr Lys His Thr Glu 355 cag cgc ttt gca gat cgc Gin Arg Phe Ala Asp Arg 345 gcc acc Ala Thr 360 gca gca Ala Ala caa Gin gcg gtg Ala Val 365 370 atg ata aac gca tcc acc gcc tac acc gat Met Ilie Asn Ala Ser Thr Ala Tyr Thr Asp 380 385 ggc gcg gag atc ggc att tcc acc cag aaa Giy Ala Glu Ile Gly Ile Ser Thr Gin Lys 400 405 cag tac ggc atg Gin Tyr Gly Met 395 gca cgt gga cca Ala Arg Gly Pro atg gcc ctg cca gag Met Ala Leu Pro Giu 415 gga caa att agg cct Gly Gin Ile Arg Pro 430 ctg acc tcc acc aag Leu Thr Ser Thr Lys 420 taagtttgaa gaagtaatca ctg cag Leu Gin 425 aca Thr agc 1419 <210> 292 <211> 432 <212> PRT <213> Corynebacterium glutamicum <400> 292 Met Ser Ser Thr Thr Leu Thr Asp Asp Gin Ile Arg Asp Asn Giu Arg 1 5 10 WO 01/00843 Thr Giu Val Ile Ala Val Ala Asp Giu Ser Asp Ile Asp Arg Leu Leu Arg Gin Gly His Val 115 Leu Gly Vai 130 ASP Ala Phe 145 Giy Ser Ser Gin Asp Vai Leu Pro Cys 195 Giy Leu Val 210 Ala Val Vai 225 Asn Cys His Aia Met Vai Thr Glu Thr 275 Ala Val Val 290 Val Ala Glu 305 Giu Thr Asp Leu Leu Leu Giu Ala Val1 100 Met Met Gly Thr Leu 180 Gin Asp Thr Phe Ile 260 Al a Gin Leu Trp, Al a Gly Val Ala Leu Ala Glu Gly Leu Ala 165 Glu Thr Val Gly Tyr 245 Asn Leu Ala Glu Asp 325 Al a Asn Giu Gly Ile 90 Pro Met Ala Gly Thr 170 Pro Gin Gly Thr Ala 250 Arg Leu Gly Thr Ser 330 Lys Ala Ile Met 75 Giu Val1 Lys Arg Asn 155 Lys Arg Asp Gly Ile 235 Lys Cys Ser Val1 Asp 315 Phe Asn Ile Ile Glu Gly Gly Gin Pro 140 Val Leu Giu Leu Ala 220 Giu Leu Ser Asp Thr 300 Vai Asp Ile Leu Glu Glu Ile Glu Val1 125 Asn Ala Vai Thr Ile 205 Gly Thr Asp Val Ser 285 Ile Val1 Ile PCT/1B00100923 Pro Asp Ala Ala Asn Ala Met Ile Gly Gly Leu Arg Val Pro Thr Val Leu Arg 160 Ile Leu 175 Gln Leu Ala Arg Ile Asn Thr Giy 240 Ala Ile 255 Asn Aia Lys Leu Gly Arg Ala Thr 320 Val Ala 335 Val Val Asp Gly Val Asp Giy Aia Leu Aia His Ile Ala Lys Tyr Ser WO 01/00843 WO 0100843PCT/L000100923 Thr Arg Thr 385 Ile Leu Giu Ala Ile Ala 360 Arg Val Asp Ala 375 Asp Gly Giu Gin 390 Lys Leu His Ala 405 Lys Trp Ile Leu 345 Thr Gin Asn Ile Giu 365 Ala Ala Val Met Ile 380 Tyr Gly Met Gly Ala 395 Arg Gly Pro Met Ala 410 Gin Giy Thr Giy Gin 425 Ala Ala Ile Pro 415 Arg <210> 293 <211> 724 <212> DNA <213> Corynebacteriun giutamicum <22 0> <221> CDS <222> (101)..(709) <223> FRXA02378 <400> 293 gagcagatgg ccaccgaggt. cgatgtggtg gctggctact cgacactgtt gcttcttaag ggtctgtata gtgggcaacc cta Leu aaa Lys acc Thr gca Ala gg t Gly gat Asp act Thr gca Ala gga Giy cgc Arg cgc Arg gaa Glu gat

ASP

act Thr ccg Pro agc Ser gca Ala tc t Ser gac caa Asp Gin gca gct Ala Ala aag aac Lys Asn gca gaa Ala Glu aac ggc Asn Gly cgc att Arg Ile att Ile aag Lys gca Alaatc Ile atg Met 75 gag Glu gac Asp atc Ile c tg Leu 45 gaa Giu gaa Glu atc Ile aa t Asn gtC Val1 30 cgt Arg gcc Ala tcC Ser gct Ala gag Glu i5 ccg Pro gcg Ala aac Asn atg Met 9gc Gly 95 cgg Arg ga t Asp gcg Ala gc t Ala att Ile 80 ggt Gly aggcctttta tggtgtgatc atg agt tca acg acc Met Ser Ser Thr Thr 1 acc gaa gtt cta gct Thr Giu Val Leu Ala att gca gtg ttg ggc Ile Ala Val Leu Gly gca gat gaa ctc gtt Ala Asp Glu Leu Val tcc gat atc gaa gcg Ser Asp Ile Giu Ala gat cgc ctt gcc ctt Asp Arg Leu Ala Leu ttg cgc cag gtt gct Leu Arg Gin Val Ala 100 WO 01/00843 WO 1/084 PCT/EBOO/00923 ggc ctg acc gac cca gtg ggt gaa gta ctg Gly Leu Thr Asp Pro Val Gly Giu Val Leu 105 110 aac ggc att cag atg aag cag gtc cgt gtg Asn Gly Ile Gin Met Lys Gin Val Arg Val 120 125 atg gtc tat gaa gcc cgc cct aac gtc acc Met Val Tyr Giu Ala Arg Pro Asn Val Thr 135 140 gca ctc aag tcc gga aac gta gct ttg ctg Ala Leu Lys Ser Gly Asn Val Ala Leu Leu 150 155 gtg cat tcc aac acc aag ctc gtg gaa atc Val His Ser Asn Thr Lys Leu Val Giu Ile 1 170 175 cgt ttc gag ctg cca cgc gaa acc gtg cag I Arg Phe Glu Leu Pro Arg Glu Thr Val Gin 185 190 gcg gat ccg tcc aag att tgatcaccgg acgcg Ala Asp Pro Ser Lys Ile 200 <210> 294 <2i1> 203 <212> PRT <213> Cory-nebacterium giutamicum <400> 294 Met Ser Ser Thr Thr Leu Thr Asp Asp Gin 1 5 10 Thr Glu Vai Leu Aia Lys Ala Thr Ala Ala 1 25 Ile Ala Val Leu Gly Thr Gly.Pro Lys Asn 40 Ala Asp Glu Leu Val Ala Arg Ser Ala Glu Ser Asp Ile Giu Ala Gly Arg Ala Asn Gly1 Asp Arg Leu Ala Leu Asp Glu Ser Arg Ile 90 Leu Arg Gin Val Ala Gly Leu Thr Asp Pro I 100 105 Giy His Val Met Giu Asn Gly Ile Gin Met 1 115 120 Leu Gly Val Met Gly Met Vai Tyr Giu Ala 2 gga G ly t tg Leu gac Asp 145 gg t Gly cag Gin ctg Leu cat His ggc Gly 130 gcc Ala tcc Ser gac Asp ctt Leu gtc Val1 115 g tg Val t tc Phe tcc Ser gta Val1 gcc Ala 195 atg gaa Met Giu atg ggc Met Gly ggc ctg Gly Leu aca gct Thr Ala 165 ctc gag Leu Giu 180 aaa ccc Lys Pro Asp Ile Leu Glu Giu Ile Glu Val1 125 Asn Asn Val1 Arg Ala Ser Ala Val1 110 Arg Val1 Giu Pro Ala Asn Met Gly Leu Val1 Thr WO 01/00843 PCTABOO/00923 130 135 140 Asp Ala Phe Gly Leu Ala Leu Lys Ser Gly Asn Val Ala Leu Leu Arg 145 150 155 160 Gly Ser Ser Thr Ala Val His Ser Asn Thr Lys Leu Val Glu Ile Leu 165 170 175 Gin Asp Val Leu Giu Arg Phe Glu Leu Pro Arg Glu Thr Val Gin Leu 180 185 190 Leu Leu Ala Lys Pro Ala Asp Pro SeZ- Lys Ile 195 200 <210> 295 <211> 623 <212> DNA <213> Corynebacterium glutanicun <220> <221> CDS <222> <223> FRXA02382 <400> 295 ccc Pro 1 gaa Glu cgc Arg gcc Ala gca Al a gca Ala acc Thr gcc Ala cgc Arg c tc Leu gga Gly acc Thr at t Ile aag Lys tgc Cys agc Ser gtc Val1 gac Asp gaa Giu ctt Leu agc Ser gac Asp aca Thr g tg Val1 gat Asp 100 atc Ile att Ile ggc Gly cag Gin tgc Cys gac Asp cat His 70 gaa Glu gc t Al a aag Lys ac c Thr ggc Gly atc Ile gc t Ala ctt Leu cgg Arg act Thr aag Lys gga Gly gca Ala gtc Val tac Tyr gc t Ala 135 tgc Cys 10 atg Met gaa Giu gtc Val gcc Ala act Thr gt t Val1 aag

LYS

ttt Phe cac His gtc Val acc Thr gtc Val gaa Giu 75 gac Asp gac Asp cac His gca Ala gat Asp aag Lys gac Asp cag Gin t tc Phe gag Giu gga Gly atc Ile gat Asp gcc Ala acc Thr gcc Ala gaa Giu gg t Gly tac Tyr gc t Ala gcc Ala gca Ala 48 96 144 192 240 288 336 384 432 ctg tcc ttc Leu Ser Phe ctg Leu acc Thr gca cac Ala His 115 caa aac Gin Asn 130 WO 01/00843 WO 0100843PCTIIIBOO/00923 gca Ala 145 tac Tyr cgt Arg gcg gtg Ala Val ggc atg Gly Met gga cca Gly Pro ata aac gca Ile Asn Ala 150 gcg gag atc Ala Glu Ile 165 gcc ctg cca Ala Leu Pro caa att agg Gin Ile Arg tcc Ser ggc Gly gag Glu cc t Pro 200 acc gcc tac acc gat ggg gag Thr Ala Tyr Thr Asp Gly Giu.

155 att tcc acc cag aaa ctg cat Ile Ser Thr Gln Lys Leu His 170 175 ctg acc tcc acc aag tgg att Leu Thr Ser Thr Lys Trp Ile 185 190 taagtttgaa gaagtaatca agc 480 528 576 cag ggc aca Gin Gly Thr 195 <210> 296 <211> 200 <212> PRT <213> Corynebacterium giutamicum <400> 296 Pro Thr Ilie Giu Thr Gly Thr Giy Asn 1 Giu Ala Lys Leu Arg Arg Cys Ser Ala Leu Ser Asp Ala Gly Val Thr Ala Thr Asp Val Leu Ser Phe Asp 100 Leu Ala His Ilie 115 Thr Gin Asn Ile 130 Ala Ala Val Met 145 Tyr Gly Met Gly Arg Gly Pro Met 180 Gin Cys Asp His 70 Giu Ala Lys Thr Asn 150 Giu Leu Ala Asn Lys Gly Ala Val1 Tyr Ala 135 Ala Ile Pro Ala 25 Thr Ala Val Giu Val 105 Thr Arg Thr Ilie Leu 185 Cys Met Giu Val1 Ala Thr Val1 Lys Phe Ala Ser 170 Thr His Val1 Thr Val1 Glu Asp Asp His Ala Tyr 155 Thr Ser Tyr Asn Leu Ala Giu Asp Val Giu 125 Arg Asp Lys Lys Ile Gly Leu Leu Ala Ser Asp 110 Ala Val1 Gly Leu Trp 190 Gin Gly Thr Gly Gin Ile Arg Pro WO 01/00843 WO 0100843PCT/IBOO/00923 200 <210> 297 <211> 933 <212> DNA <213> Corynebacteriun glutanicum <220> <221> CDS <222> (101)..(910) <223> RXA02499 <400> 297 cctggtcatg acgttcgggc cgctggcaat cgtcatgtaa tttgtcgttt tgggcccccg ctgctgcaaa acaggggtgg ttagtggcag tgtgggaacc atg aca aca att gct gta Val1 gcg Ala gag Glu ga t Asp aag Lys ga t Asp agc Ser cgc Arg gtc Val gac Asp 150 atc Ile gcc Ala cgc Arg aat Asn ccg Pro aac Asn atc Ile g tc Val1 acc Thr 135 t tg Leu ggc Gly aac Asn ggc Gly tcc Ser aaa Lys aac Asn gc t Ala atg Met 120 aaa Lys ttg Leu caa Gin cca Pro c tg Leu gca Ala gtc Val 75 caa Gin gaa Glu act Thr tac Tyr gt t Val 155 a tc Ile caa Gin cgt Arg gac Asp 60 gaa Giu agt Ser gaa Giu cc a Pro gtt Val1 140 gga Giy gag Giu att Ile cgc Arg gcc Aia ctc Leu gtg Val gcc Ala 110 ctc Leu gcg Ala gtc Val1 gct tta Ala Leu cgc gtc Arg Val tac ggc Tyr Gly gac gtg Asp Val tcc gaa Ser Giu 80 gtc agc Val Ser tc gcg Ser Ala gtg ggc Val Gly gaa cag Glu Gin ctc gaa Leu Glu 160 Met 1 gtc Val1 acc Thr atc Ile gtg Val atc Ile atg Met ggg Gly aag Lys t tg Leu 145 gtc Val Thr tca Ser aac Asn cc Leu t tc Phe acc Thr gcc Ala cc Leu ggc G ly 130 gaa Giu gcg Ala Thr ggt Gly cg t Arg aac Asn c tg Leu ggc Gly gca Ala ccc Pro 115 atg Met caa Gin gaa Glu Aia atc Ile gaa Glu acg Thr gtg Val t tg Leu atc Ile gtg Val1 act Thr aag Lys gac Asp 165 115 163 211 259 307 355 403 451 499 547 595 643 atc gac gca gtc acc gcg atg tcc gga tcc tcc cct gca tac ctg ttc WO 01/00843 Ilie Asp Ala Val Thr Ala Met Ser C 170 ctt gtg acc gaa gcg ctc att gag q Leu Val Thr Glu Ala Leu Ile Giu .1 185 1 cgc gcg acc gCt aaa aag ctc gct q Arg Ala Thr Ala Lys Lys Leu Ala 200 205 acc atg atg aag gaa acc ggc aaa q Thr Met Met Lys Glu Thr Gly Lys C 215 220 gtt tcc tca ccc gca ggc acc acc Val Ser Ser Pro Ala Gly Thr Thr N 230 235 gaa agc gga atc cga ggc gct ttct Glu Ser Gly Ile Arg Gly Ala Phe 250 gac cga tct gaa gaa ctc gga aag c Asp Arg Ser Giu Glu Leu Gly Lys 265 tta <210> 298 <211> 270 <212> PRT <213> Corynebacteriun glutamicun <400> 298 Met Thr Thr Ilie Ala Val Ilie GlyC 1 5 Val Ser Gly Leu Ile Ala Ala Asn 1 Thr Asn Arg Ser Glu Glu Arg Gly Ile Leu Asn Met Thr Asp Asn Ser Val Phe Leu Cys Vai Lys Pro Lys Ilie Thr Gly Thr Leu Asp Asn Asn Met Ala Ala Gly Ile Ser Ile Ala 100 Gly Leu Pro Val Val Arg Val MetI 115 120 Lys Gly Met Ser Thr Val Thr Lys ,iy [ca lia .90 [tg ral ~aa ;iu ;tc al1 :ac yr ~gc ~rg PCTJLBOOIOO923 Ser Ser Pro Ala Tyr Leu Phe 175 180 gga gtt aat cta ggc ctg ccc 691 Gly Val Asn Leu Gly Leu Pro 195 gcc tca ttc gaa ggt gct gca 739 Ala Ser Phe Giu Gly Ala Ala 210 ccc tca gaa ttg cgc gca ggc 787 Pro Ser Glu Leu Arg Ala Gly 225 gca gcc atc cga gaa ctc gaa 835 Ala Ala Ile Arg Glu Leu Giu 240 245 cgc gca gcc caa gct tgc gcc 883 Arg Ala Ala Gin Ala Cys Ala 255 260 tagaaaccgt tatttccccg 930 ;iy e t 25 1n ;ln ?he ;er kla L05 ro ;Ay 434 Giu Ile Artg Ala Leu Val1 Ala 110 Leu Al a Ala Arg Tyr Asp Ser Val1 Ser Val1 Glu WO 01/00843 130 Leu Giu Gi 145 Val Ala Gi Pro Ala Ty' Asn Leu Gi 19 Phe Glu GI 210 Giu Leu Ax 225 Ilie Arg GI Ala Gin Al PCTIiBOOIOO923 Asp 150 Ile Leu Arg Thr Val1 230 Giu Asp 135 Leu Asp Val Ala Met 215 Ser Ser Arg Asp Ser Giu Ala 205 Lys Thr Phe Lys Val1 Gly Ala 190 Val1 Giu Val Tyr Arg 270 <210> 299 <211> 1296 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (101)..(1273) <223> RXS02157 <400> 299 gggtggaatt ggCacgatgg tgctgccgga tgtttttgat caccgttttt agaaaagacg acaaggatgg ggaactgtaa att Ile ggC Gly gcg Ala gag Glu ttc acg Thr gtc Val gca Ala acc Thr agg 435 cgggagaatt atcctgaagg atg agc acg ctg gaa Met Ser Thr Leu Giu 1 acc cca cca gtt gag Thr Pro Pro Val Giu gac cag ggc aat gtc Asp Gin Gly Asn Val gcg ttg ggc cac gcc Ala Leu Gly His Ala atc ggc caa ctt ggt Ile Gly Gin Leu Gly gtc gag gtc gcc gag WO 01/00843 WO 0100843PCTJIBOOIOO923 His Val gag ctc Glu Leu acc cgg Thr Arg ttc aag Phe Lys cat ggt His Gly 135 cca gac Pro Asp 150 tac cct Tyr Pro cca acg Pro Thr ggc gtt Gly Val tgc gat Cys. Asp 215 gtt ggc Val Gly 230 ccc gat Pro Asp ggt gct Gly Ala aag cac Lys His aag gca 1027 Ser atc Ile gtt Val att Ile 120 t tc Phe aag Lys tac Tyr ga t Asp gtt Val 200 gag Glu cgt Arg gtg Val1 tgt Cys ggc G ly 280 gtg Asn Leu Phe Ala Ser Arg Pro Val Val Glu Val 7 5. 80 aag Lys ttc Phe 105 gca Ala cac His cgt Arg ggc Gly gtg Val1 185 cca Pro tac Tyr acc Thr g tg Val t tg Leu 265 acc Thr ctg cgt Arg 90 t tc Phe cgc Arg ggc Gly gaa Glu gac Asp 170 gct Ala gca Al a ggc Gly ggc Gly acc Thr 250 gc c Ala act Thr tc t ttt Phe tgc Cys t tg Leu cgc Arg gcg Ala 155 acc Thr gc t Ala cc t Pro atc Ile ga t Asp 235 atg Met act Thr ttc Phe gtt gac Asp 95 gcc Ala tcc Ser tcc Ser a tg Met cgc Arg 175 gag Glu ctc Leu ac c Thr cat His ctt Leu 255 gc t Al a cca Pro gc t gcc Ala gaa Glu cgg Arg ctc Leu cca Pro 160 aaa Lys cca Pro aag Lys ga t

ASP

cag Gin 240 9gc Gly gaa Glu gtt Val t tc acc Thr gca Ala att Ile gcg Ala 145 agc Ser atg Met atc Ile gca Ala gaa Glu 225 cac His 9gc Gly t tg Leu gc t Ala tgc ctc Leu aac Asn ctg Leu 130 ctg Leu gg t Gly gta Val cag Gin g tg Val1 210 gtc Val1 ga t Asp ggt Gly atg Met tg t Cys 290 gca gcc Ala gag Giu 115 gc t Ala act Thr gtg Val gaa Glu gg t Gly 195 cgc Arg cag Gin Gly ctt Leu acc Thr 275 gca Ala gaa Ala gcg Ala 100 gc t Ala gca Al a ggc Gly gag Glu acc Thr 180 gaa Glu gag Glu act Thr gtt Val ccc Pro 260 cca Pro gc t Ala gtt Glu caa Gin gc t Ala gtt Val cag Gin t tc Phe 165 aac Asn acg Thr ctg Leu ggc Gly gt t Val1 245 atc Ile Gly gcc Ala gcc Lys Ala Val Leu Ser Val Val Asp Asp Ala Phe Cys Ala Giu Val Ala WO 01/00843 cgc aag ggc gag ctg ttc aag gaa ctt 1075 Arg Lys Gly Glu Leu Phe Lys Giu Leu 310 315 gta gac gtc cgt ggc agg ggc ttg atg 1123 Val Asp Val Arg Gly Arg Gly Leu Met 330 gac gtc gca aag caa gct gtt ctt gat 1171 Asp Val Ala Lys Gin Ala Val Leu Asp 345 350 ttg aat gca ccg gcg gac aac att atc 1219 Leu Asn Ala Pro Ala Asp Asn Ile Ile 360 365 atc acc gac gaa gaa atc gca gac gca 1267 Ile Thr Asp Glu Glu Ile Ala Asp Ala 375 380 atc gca taaaggactc aaacttatga ctt 1296 Ile Ala 390 <210> 300 <211> 391 <212> PRT <213> Corynebacterium glutamicum <400> 300 Met Ser Thr Leu Glu Thr Trp, Pro Gin 1 5 Thr Pro Pro Val Glu Leu Val Ser Gly Asp Gin Gly Asn Val Ty'r Ile Asp Leu Ala Leu Gly His Ala His Pro Ala Ile 55 Ile Gly Gin Leu Gly His Val Ser Asn 70 Val Giu Val Ala Glu Glu Leu Ile Lys Thr Leu Ala Ala Gin Thr Arg Val Phe 100 105 Ala Asn Glu Ala Ala Phe Lys Ile Ala 115 120 ctt Leu t tg Leu 335 ggt Gly cgt Arg gtc Val1 Val Lys Leu Ile Leu Arg Phe Arg gcc Ala 320 ggC Gly ttt Phe ttg Leu aag Lys Ile Giy Ala Giu Phe 75 Phe Cys Leu aag Lys gtg Val aag Lys acc Thr gc t Ala 385 Ile Ala Gly Ala Ala Ser Asn Thr PCTIIBOO/00923 ggc gtt Gly Val 325 gag cgc Glu Arg 340 gtt att Val Ile ctg gtg Leu Val gag aca Giu Thr Gly Asp Asn Gin Val Ala Giu Arg WO 01/00843 PCTIBOOIOO923 Ala Vai His Gly Phe His Gly Arg Thr Met Gly Ser Leu Ile Ala 145 Ser Met Ilie Ala Giu 225 His Gly Leu Ala Cys 305 Lys Val1 Lys Thr Ala 385 Leu 130 Leu Gly Val Gin Val1 210 Val Asp Gly Met Cys 290 Ala Val Val1 His Pro 370 Ile Ala Thr Val1 Giu Gly 195 Arg Gin Gly Leu Thr 275 Ala Glu Asp Leu Gly 355 Pro Ala Gly Giu Thr 180 Giu Giu Thr Val Pro 260 Pro Ala Val1 Gly Giu 340 Vai Leu Glu Gin Phe 165 Asn Thr Leu Gly Val1 245 Ile Gly Ala Ala Val 325 Arg Ilie Val1 Thr 135 Asp Pro Thr Val1 Asp 215 Gly Asp Ala His Ala 295 Lys Asp Val1 Asn Thr 375 Al a 140 Lys Tyr Asp Val1 200 Giu Arg Val Cys Gly 280 Val1 Gly Val Ala Ala 360 Asp Phe Asp Ile Giu Leu 220 Phe Ala Gly Gly Val 300 Lys Gly Val1 Asn Ala 380 Leu Tyr Phe dly 205 Met Phe Lys Arg Gly 285 Asp Giu Leu Leu Ile 365 Asp Met Arg 175 Giu Leu Thr His Leu 255 Ala Pro Ala Leu Leu 335 Gly Arg Val1 <210> 301 <211> 1269 <212> DNA <213> Corynebacterium giutainicum <220> <221> CDS <222> (101)..(1246) <223> RXS02262 WO 01/00843 WO 0100843PCTJIBOO/00923 <400> 301 gcaccaattt cggacctgaa atccccgagg aaaccgtgcc cgacgccgtg caggtgggcg tcgataagca aaaaatcgct gatactcgaa aggcctcaaa atg acc gca acc tac Met Thr Ala Thr Tyr acc Thr atc Ile gaa Glu aac Asn ttt Phe ccc Pro gga Gly acc Thr tcc Ser ggC Gly 150 gga Gly cgc Arg atc Ile gcc Ala gtc Val1 ctc Leu cat His ccc Pro ggc Gly gaa Glu aac Asn ctg Leu tta Leu atg Met 170 acc Thr gca Ala a tc Ile aaa Lys t tg Leu ggC Gly aag Lys 75 ggg Gly aac Asn gac Asp tcC Ser gcg Ala 155 gcg Ala acc Thr aca Thr aa t Asn gac Asp gcg Al a gcg Ala gac Asp cga Arg aag Lys acg Thr gcc Ala 140 gtc Val1 aaa Lys a tc Ile tgg Trp ttC Phe g tc Val cag Gin a tg Met ggc Gly ttc Phe gcc Ala gtc Val1 125 t ac Tyr gcc Ala acc Thr aag Lys gcg Ala 205 t tg Leu gcg Ala ggc Gly atc Ile ccc Pro aaa Lys tca Ser 110 acc Thr cgc Arg gac Asp atc Ile atc Ile 190 t tg Leu t tg Leu tgc Cys tac Tyr t tc Phe cgc Arg 80 acc Thr ttg Leu gca Ala ggc Gly aca Thr 160 gaa Giu gga Gly aaa Lys 1 agc Ser gtc Val aaa Lys ccg Pro 65 cga Arg ggc Gly cct Pro ccg Pro gcg Ala 145 ac c Thr gcg Ala cgc Arg t tC Phe gaa Giu ga t Asp a tg Met 50 gaa Glu ttC Phe g tg Val cgc Arg aag Lys 130 gtt Val1 t tg Leu tgc Cys agc Ser ccc Pro 210 ccg Pro gtC Val 35 gcc Ala aac Asn atg Met aag Lys tcg Ser 115 gcc Ala ccc Pro gc t Ala atc Ile gaa Glu 195 gag Glu gac Asp 20 atg Met gg t Gly cca Pro gcg Ala tgg Trp 100 atc Ile atc Ile ggc Gly gtc Val1 gca Ala 180 cgc Arg atc Ile atg Met gag Giu t tg Leu gaa Glu atg Met tac Tyr cac His atg Met g tg Val gtc Val 165 gag Giu Gly gaa Glu 115 163 211 259 307 355 403 451 499 547 595 643 691 739 787 gtg gtc gcc gtc gga tct gaa gaa gac gtg gtc aaa gac gcc gac atc WO 01/00843 PCTIiBOO/00923 Val Val Ala Val Gly Ser Glu Glu Asp Val Val Lys Asp Ala Asp Ile 215 220 225 gtc atc gcc gcc acc acc acg gac gcc gcc ggc tcc tcc gcc ttc cca 835 Val Ilie Ala Ala Thr Thr Thr Asp Ala Ala Gly Ser Ser Ala Phe Pro 230 235 240 245 tac ttc aaa aaa gaa tgg ctc aag ccg ggc gca ttg ctg ctg ctt cca 883 Tyr Phe Lys Lys GiU Trp Leu Lys Pro Giy Ala Leu Leu Leu Leu Pro 250 255 260 gcc gcc ggt cgc ttc gac gac gct tat ttg ctt gac gac gcc cgc ctc 931 Ala Ala Gly Arg Phe Asp Asp Ala Tyr Leu Leu Asp Asp Ala Arg Leu 265 270 275 gtt gtt gac tac atg ggg ctc tac gaa gcc tgg gca gaa gaa tac ggc 979 Val Val Asp Tyr Met Gly Leu Tyr Giu Ala Trp Ala Giu Giu Tyr Gly 280 285 290 cca cag gcc tac caa cta ctc ggc att cca gga acc cac tgg tac gac 1027 Pro Gin Ala Tyr Gin Leu Leu Gly'Iie Pro Gly Thr His Trp Tyr Asp 295 300 305 ctg gcg ctg caa gga aaa ctc gac ctt gca aag att tcc cag att ggc 1075 Leu Ala Leu Gin Gly Lys Leu Asp Leu Ala Lys Ile Ser Gin Ile Gly 310 315 320 325 gat atc tgc tcc ggc aag cta ccc gga cgc acc aac gat gag gaa atc 1123 Asp Ilie Cys Ser Gly Lys Leu Pro Gly Arg Thr Asn Asp Giu Giu Ile 330 335 340 atc ctc tat tcc gtc ggc ggc atg cca gta gaa gac gtc gcc tgg gca 1171 Ile Leu Tyr Ser Val Gly Gly Met Pro Val Giu Asp Val Ala Trp Ala 345 350 355 acc caa gtg tat gaa aac gcc ctg gaa aaa ggc gtc ggc acc aca ttg 1219 Thr Gin Val Tyr GiU Asn Ala Leu Giu Lys Gly Val Gly Thr Thr Leu 360 365 370 aac ctg tgg gaa tca ccc gca ctg gct tgagagaaga aacaacaatg 1266 Asn Leu Trp .Giu Ser Pro Ala Leu Ala 375 380 aaa 1269 <210> 302 <211> 382 <212> PRT <213> Corynebacteriun giutanicuni <400> 302 Met Thr Ala Thr Tyr Thr Thr Giu Thr Ala Ilie Asn Phe Leu Phe Leu 1 5 10 WO 01/00843 WO 0100843PCT11B00100923 Ser Val Lys Pro Arg Gly Pro Pro Ala 145 Thr Ala Arg Phe Lys 225 Ser Leu Asp Ala Thr 305 Ile Glu Asp Met Giu Phe Val Arg Lys 130 Val Leu Cys Ser Pro 210 Asp Ser Leu Asp Glu 290 His Ser Pro Val Ala Asn Met Lys Ser 115 Ala Pro Ala Ile Glu 195 Glu Ala Ala Leu Ala 275 Giu Trp, Gin Asp Met Gly Pro Ala Trp 100 Ile Ile Gly Val1 Ala 180 Arg Ile Asp Phe Leu 260 Arg Tyr Tyr Ile Met Glu Leu Glu Met Tyr His Met Val1 Val1 165 Giu Gly Giu Ile Pro 245 Pro Leu Gly Asp Gly 325 Ile Glu Asn Phe 70 Pro Gly Thr Ser Gly 150 Gly Arg Ile Val Val1 230 Tyr Ala Val1 Pro Leu 310 Asp Val Leu His Pro Gly 90 Glu Asn Leu Leu Met 170 Thr Ala Gly Thr Giu 250 Phe Met Gin Gly Gly 330 Lys Leu Gly Lys 75 Gly Asn Asp Ser Ala 155 Ala Thr Thr Ser Thr 235 Trp Asp Gly Leu Lys 315 Lys Asp Ala Ala Asp Arg Lys Thr Ala 140 Val1 Lys Ile Trp Giu 220 Thr Leu Asp Leu Leu 300 Leu Leu Ala Gly Ile Pro Lys Ser 110 Thr Arg Asp Ile Ile 190 Leu Asp Ala Pro Tyr 270 Giu Ile Leu Giy Gin Asp Thr Asp Asn Gly Gly Thr Ala Thr 175 Lys Glu Val1 Ala Gly 255 Leu Ala Pro Ala Arg 335 Cys Tyr Phe Arg Thr Leu Ala Gly Thr 160 Glu Gly Lys Val1 Giy 240 Ala Leu Trp Gly Lys 320 Thr WO 01/00843 PCTIiBOOIOO923 Asn Asp Giu Giu Ile Ile Leu Tyr Ser Val Gly Gly Met Pro Val Giu 340 345 350 Asp Val Ala Trp Ala Thr Gin Val Tyr Giu Asn Ala Leu Giu Lys Gly 355 360 365 Vai Gly Thr Thr Leu Asn Leu Trp Glu Ser Pro Ala Leu Ala 370 375 380 <210> 303 <211> 1491 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1468) <223> RXS02970 <400> 303 aaccgacaaa acagccgttc acgtgctaaa gcagctcggc ttgatctagg gtgaggtgag ttatttaaag acttcataat attttgggga gtgaactggt ttg gca Leu Ala ttg aag ggt Leu Lys Gly tac Tyr aaa Lys cac His gcc Ala atg Met t ta Leu ccg Pro tcg Ser gcc acc Thr gaa C iu tcc Ser gaa Glu ggt Gly gtt Val1 gcc Ala a tg Met gac aac Asn gag Giu tgg Trp ggt Gly tcc Ser gag Giu ttc Phe gcc Ala 120 gcc gg t Gly Cgg Arg cag Gin c tg Leu gtc Val1 75 cag Gin ga t Asp gaa Giu cac gaa Glu gca Ala gac Asp tac Tyr 60 tcg Ser cgc Arg gtg Val ttc Phe tcc atc Ile gac

ASP

a tc Ile ttc Phe aac Asn gca Ala tct Ser 110 cac His cgc t tc Phe gat Asp ccc Pro ggc Gly 9gc G ly 80 cgg Arg gt t Val1 ttt Phe gct gga Gly cgc Arg aaa Lys aac Asn 65 cac His t tg Leu gc t Ala ttc Phe cgc gtg Val1 c ac His 35 tgg Trp ttc Phe aac Asn aac Asn aag Lys 115 aac Asn cac caa Gin gtt Val gca Ala atc Ile cc t Pro atc Ile 100 atc Ile Gly ac c gca Ala t tc Phe gc t Ala gac Asp cga Arg aac Asn gtg Val1 Gly gga 115 163 211 259 307 355 403 451 499 547 Ala Asp Ala Ile Glu His Ser Ile Arg Met Ala Arg Leu His Thr Gly WO 01/00843 WO 0100843PCT/EBOO/00923 135 aac Asn aaa att ctg Lys Ile Leu tac cgc agc Tyr Arg Ser ggc gca acc Gly Ala Thr gga Gly 165 gcg atg atg Ala Met Met ggc gaa cac Gly Glu His cgc Arg 175 gca Ala ctg ggc aac Leu Gly Asn ccc acc Pro Thr 180 acc gac cca Thr Asp Pro tca ttc ttt Ser Phe Phe 200 tac cac ttc Tyr His Phe tgg Trp 190 gaa Glu cca ttc ctg Pro Phe Leu acc acc caa Thr Thr Gin gaa tgc gaa Glu Cys Giu cac cac tcc His His Ser 195 gca ctc aag Ala Leu Lys atc gca gcg Ile Ala Ala cac ttg His Leu 215 atc gtc Ile Val gaa gat gtc atc Giu Asp Val Ile gaa ggt gct Glu Gly Ala 595 643 691 739 787 835 883 931 979 ctg gag cca Leu Giu Pro gga tca tca Gly Ser Ser ggt tac tta Gly Tyr Leu ggc gtg cgc gaa Gly Val Arg Glu ctt Leu 255 gga Gly gga atc atc Gly Ile Ile 240 tgc aac aag Cys Asn Lys ctg cca Leu Pro cca Pro 245 cac His ggc atc Gly Ile 260 ctc ttc atc Leu Phe Ile gaa gtc atg Giu Val Met ttc gga Phe Gly ctg ttt gct tac Leu Phe Ala Tyr 280 acc ttc 1027 Thr Phe 295 gtg atg 1075 Val Met 310 ggc gga 1123 Gly Gly gca gcg 1171 Ala Ala cga ctt 1219 Arg Leu gcc aag Ala Lys acc caa Thr Gin gag cat gct Giu His Ala ggt gtt aac Gly Val Asn 300 tca atc cgc Ser Ile Arg gat ttc cag Asp Phe Gin cgc acc gga aaa Arg Thr Gly Lys 275 cca gac atg atc Pro Asp Met Ile 290 ctc ggt ggc atc Leu Gly Gly Ile ggt tac gcc Gly Tyr Ala acc ttc gga Thr Phe Gly cca Pro 305 ga t

ASP

315 320 ctc acc Leu Thr ctg gag Leu Glu 345 ggc gct Giy Ala tac Tyr 330 t cc Ser gga cac cca ctt G ly His Pro Leu 335 gca Ala tca gag gca tac tcc Ser Glu Ala Tyr Ser 325 gta gca ccc gcc aag Val Ala Pro Ala Lys 340 att cca cgc gta gct Ile Pro Arg Val Ala att tac gcg gaa gga Ile Tyr Ala Glu Gly 350 gaa ctg atc gaa cct Giu Leu Ile Giu Pro gag atc Glu Ile 355 cgc ctt cgt gaa cta gcg gaa Arg Leu Arg Glu Leu Ala Glu WO 01/00843 360 PCTJIBOO/00923 365 gaa aac gta gcg atc gct gac gtg cgg 1267 Glu Asn Val Ala Ile Ala Asp Val Arg 375 380 gtg gag ttc aat gca gac gcc act gcc 1315 Val Glu Phe Asn Ala Asp Ala Thr Ala 390 395 ttc aag gaa cgc ggc gtg tgg ccg atg 1363 Phe Lys Glu Arg Gly Val Trp, Pro Met 410 atc gcg ccg ccg ctg acc acc act gat 1411 Ile Ala Pro Pro Leu Thr Thr Thr Asp 425 430 gac gcg gtg gaa gct gca gcc caa gct 1459 Asp Ala Val Giu Ala Ala Ala Gin Ala 440 445 gcg ttg ttc taagttttct agataacaag gc 1491 Ala Leu Phe 455 <210> 304 <211> 456 <212> PRT <213> Corynebacterium glutamicumr <400> 304 Leu Ala Leu Lys Gly Tyr Thr Asn Phe 1 5 Gly Ser Val Gin Ala Lys Giu Giu Giu 25 Arg Ala His Val Phe His Ser Trp Ser 40 Lys Val Trp Ala Ala Ala Giu Giy Ser 55 Asn Ala Phe Ile Asp Met Gly Ser Gin 70 His Asn Asn Pro Arg Leu Vai Giu Ala Leu Thr Asn Ilie Asn Pro Ala Phe Gly 100 105 Ala Ala Lys Ile Val Ser Met Ala Arg 444 ggC Gly atg Met atc Ile 415 gac Asp gtc Val1 a tc Ile gct Ala 400 tcc Ser gaa Giu gag Giu Gly Arg Gin Leu Val1 Gin Asp Glu gga Gly 385 gcc Ala ggC Gly t tg Leu c tg Leu Glu Ala Asp Tyr Ser Arg Val1 Phe 370 t tc Phe ggt Gly aac Asn gta Val1 acc Thr 450 Phe Phe Lys Asp Ala Gin Arg Ser ttC Phe gc t Ala cga Arg gca Ala 435 t tc Phe Ile Asp Ile Phe Asn Ala Ser 110 His tgg Trp gca Al a ttc Phe 420 ctg Leu gct Ala Glu Asn Ser Asp Leu Ala Asp Val1 WO 01/00843 WO 0100843PCTIIBOOIOO923 115 120 Phe Thr Asn Giy Gly Ala Asp Ala Ile Glu His Ser Arg 145 His Leu Phe Giu Gly 225 Ile Asn Giy Gin Pro 305 Ser Val1 Ile Arg Giy 385 Ala Gly Leu 130 Leu Gly Gly Leu Arg 210 Met Ile Lys Arg Pro 290 Leu Glu Ala Pro Glu 370 Phe Gly Asn Val1 His Ala Asn His 195 Ala Ile Leu His Thr 275 Asp Gly Ala Pro Arg 355 Leu Phe Ala Arg Ala 435 Thr Thr Pro 180 His Leu Ala Pro Gly 260 Gly Met Gly Tyr Ala 340 Val1 Ala Trp Al a Phe 420 Leu Gly Gly 165 Thr Ser Lys Ala Pro 245 Ile Lys Ile Ile Ser 325 Lys Ala Giu Al a Giu 405 His Leu Arg 150 Ser Thr Ser His Ile 230 Ala Leu Leu Thr Val 310 Gly Ala Arg Giu Val 390 Phe Ile Asp 135 Asn Ala Asp Phe Leu 215 Val1 Gly Phe Phe Phe 295 Met Gly Ala Leu Asn 375 Giu Lys Ala Ala Lys Met Pro Phe 200 Glu Leu Tyr Ile Ala 280 Ala Thr Leu Leu Gly 360 Val Phe Glu Pro Val1 440 Ser 155 Thr Tyr Thr Ile Val1 235 Gly Glu His Val1 Ile 315 Ser Tyr Leu Ala Asp 395 Val1 Thr Ala 140 Ala Gly His Gln Ala 220 Val Val Val Ala Asn 300 Arg Gly Ala Ile Asp 380 Ala Trp Thr Al a 125 Ile Tyr Glu Phe Glu 205 Phe Gly Arg Met Gly 285 Ala Asp His Gi u Giu 365 Val Thr Pro Thr Gin 445 Arg His Trp 190 Glu Giu S er Glu Val 270 Asp Gly Thr Pro Gly 350 Pro Arg Ala Met Asp 430 Ala Ser Arg 175 Ala Glu Gly Ser Leu 255 Gly Asp Tyr Phe Leu 335 Giu Arg Gly Met Ile 415 Asp Val Tyr 160 Arg Pro Cys Ala Gly 240 Cys Phe Phe Ala Gly 320 Ala Ile Leu Ile Ala 400 Ser Glu Glu Arg Met Ala WO 01/00843 WO 0100843PCTIIBO0/00923 Leu Thr Phe Ala Gly Ala Leu Phe 450 455 <210> <211> <212> <213> <220> <221> <222> <223> 305 1330

DNA

Corynebacterium

CDS

(101)..(1330) FRXA01009 giutamicum <400> 305 aaccgacaaa acagccgttc acgtgctaaa gcagctCggc ttgatctagg gtgaggtgag ttatttaaag acttcataat attttgggga gtgaactggt ttg gca ttg aag ggt Leu Ala Leu Lys Gly tac acc aac ttt Tyr Thr Asn Phe ggt gaa ttc atc gaa ttc gga tct gtg Gly Glu Phe Ile Glu Phe Gly Ser Val caa gca Gin Ala aaa gaa gag Lys Glu Glu cac tcc tgg His Ser Trp aaa cgg gca ttc gac aac gat cgc gcg Lys Arg Ala Phe Asp Asn Asp Arg Ala cac gtt ttc Hius Val Phe tgg gca gct Trp Ala Ala tcc gcg cag gac Ser Ala Gin Asp atc agc ccc aaa Ile Ser. Pro Lys gcc gaa ggt tcc acg ctg tac gac ttc gac ggc aac gcc ttc atc gac Ala Giu Gly Ser Thr Leu Tyr Asp Phe Asp Gly Asn Ala Phe Ile Asp atg Met ggt tcc caa ctt Gly Ser Gin Leu gtc Val tcg gca aac tta Ser Ala Asn Leu cac aac aac cct His Asn Asn Pro tta gtt gag gcg Leu Val Giu Ala cag cgc caa gca gcc cgg ttg acc aac Gin Arg Gin Ala Ala Arg Leu Thr Asn atc aac Ile Asn 100 ccg gcc ttc Pro Ala Phe tcg atg gcc Ser Met Ala 120 aat gat gtg cgc tct gat gtt gct gca Asn Asp Val Arg Ser Asp Val Ala Ala 110 aag atc gtg Lys Ile Val 115 cgt ggc gaa ttc tcc cac gtg ttt ttc acc aac ggc ggc Arg Gly Glu Phe Ser His Val Phe Phe Thr Asn Gly Gly gcc gac Ala Asp 135 gcc atc gag cac Ala Ile Glu His tcc Ser 140 atc cgc atg gct cgc ctg cac acc gga Ile Arg met Ala Arg Leu His Thr Gly 145 aac aaa att ctg Asn Lys Ile Leu tcc Ser 155 gca tac cgc agc Ala Tyr Arg Ser tac Tyr 160 cac ggc gca acc His Gly Ala Thr WO 01/00843 WO 0100843PCTIBOOIOO923 tcc gcg Ser Ala acc gac Thr Asp tca ttc Ser Phe cac ttg His Leu 215 atc gtc Ilie Val 230 gca ggt Ala Gly ctc ttc Leu Phe ctg ttt Leu Phe acc ttc 1027 Thr Phe 295 gtg atg 1075 Val Met 310 ggc gga 1123 Gly Gly gca gcg 1171 Ala Ala cga ctt 1219 Arg Leu gaa aac 1267 atg met cca Pro ttt Phe 200 gaa Glu ctg Leu tac Tyr atc Ile gc t Ala 280 gcc Ala acc Thr c tc Leu c tg Leu ggC Gly 360 acc Thr tac Tyr acc Thr atc Ile gtg Val 235 ggC Gly gaa G lu cat His gtt Val1 atc Ile 315 tc c Ser tac Tyr ctg Leu ggc Gly cac His caa Gin gcg Ala 220 gtg Val g tg Val1 g tc Val1 gc t Ala aacd Asn 300 cgc Arg gga Giy gcg Al a atc Ile gaa Glu ttc Phe gaa Giu 205 ttt Phe gga Gly cgc Arg atg Met ggc Gly 285 gca Ala ga t Asp cac His gaa Glu gaa Glu 365 cac His t gg Trp 190 gaa Glu gaa Glu tca Ser gaa Glu gtc Vai 270 gac Asp ggt Gly acc Thr cca Pro gga Gly 350 cc t Pro cgg aac Asn cac His 195 gca Ala atc Ile c tg Leu cac His acc Thr 275 gac Asp gg t Gly gca Ala ccc Pro cgc Arg 355 c ta Leu ccc Pro 180 c ac His c tc Leu gca Ala cca Pro ggc Gly 260 gga Gly atg Met ggc Gly tac Tyr gcc Ala 340 gta Val gcg Ala acc Thr tcc Ser aag Lys gcg Ala cca Pro 245 atc Ile aaa Lys atc Ile atc Ile tcc Se r 325 aag Lys gc t Ala gaa Glu 643 691 739 787 835 883 931 979 gta gcg atc gct gac gtg atc gga, ttc ttc tgg gca Glu Asn Val Ala Ilie Ala Asp Vai Arg Gly Ile Gly Phe Phe Trp Ala WO 01/00843 375 gtg gag ttc 1315 Val Glu Phe 390 etc aag gaa 1330 Phe Lys Glu PCTIiBOO/00923 380 385 aat gca gac gcc act gcc atg gct gcc ggt gct gca gaa Asn Ala Asp Ala Thr Ala Met Ala Ala Gly Ala Ala Glu 395 400 405 cgc ggc Arg Gly 410 <210> 306 <211> 410 <212> PRT <213> Corynebacteriun glutarnicum <400> 306 Leu Ala Leu Lys Gly Tyr Thr Asn Phe Val1 His Trp, Phe Asn Asn Lys 115 Asn His Ala Asn His 195 Ala Gin Val1 Ala Ile Pro Ile 100 Ile Giy Thr Thr Pro 180 His Leu Ala Phe Ala Asp Arg Asn Val1 Gly Gly Gly 165 Thr Ser Lys Glu Ser Glu Gly Val Ala Met Asp 135 Asn Ala Asp Phe Leu 215 Asp Lys Ala Thr Leu Ile Asn Gly Giu Leu Leu 170 Ile Thr Val Phe Phe Lys Asp Ala Gln Arg Ser 125 Ile Tyr Giu Phe Giu 205 Phe Ile Asp Ile Phe Asn Ala Ser 110 His Arg Arg His Trp, 190 Glu Glu WO 01/00843 WO 0100843PCT/EBOO/00923 Gly Met Ile Ala Ala Ilie Val Leu Giu Pro Val Val Gly Ser Ser Gly 225 230 235 240 Ilie Asn Gly Gin Pro 305 Ser Val Ile Arg Gly 385 Ala Ilie Lys Arg Pro 290 Leu Glu Ala Pro Glu 370 Phe Gly Leu His Thr 275 Asp Giy Aia Pro Arg 355 Leu Phe Ala Pro Gly 260 Gly Met Gly Tyr Ala 340 Val1 Ala Trp Ala Pro 245 Ilie Lys Ile Ile Ser 325 Lys Ala Glu Ala Glu 405 Ala Leu Leu Thr Val1 310 Gly Ala Arg Glu Val 390 Phe Gly Phe Phe Phe 295 Met Gly Ala Leu Asn 375 Glu Lys Leu Ala 265 Tyr Lys Gin Thr Giu 345 Ala Ala Asn Arg Gly Glu His Val1 Ile 315 Ser Tyr Leu Ala Asp 395 Val Val1 Ala Asn 300 Arg Gly Ala Ilie

ASP

380 Ala Arg Met Gly 285 Ala Asp His Glu Glu 365 Val1 Thr Leu 255 Gly Asp Tyr Phe Leu 335 Glu Arg Gly Met <210> 307 <211> 3579 <212> DNA <213> Corynebacterium glutarnicum <220> <221> CDS <222> (101)..(3556) <223> RXN00023 <400> 307 gggattatgt tgggggccac atctgaaaat agttgctggg atccatcaca cctacgatgc gaaacggcac ccacaccgct gatcttgaag gagaaccacc atg acg tcg atg aat 115 Met Thr Ser Met Asn 1 ctg cct att gag ttg gct acg ctg tct gac cag gct gtg gac aag gtg 163 Leu Pro Ile Giu Leu Ala Thr Leu Ser Asp Gin Ala Val Asp Lys Val 15 cgc tcc tgg ctg gag tac agc aaa aag gaa agc gtg ccc aat gcc gat 211 Arg Ser Trp Leu Glu Tyr Ser Lys Lys Giu Ser Val Pro Asn Ala Asp 30 WO 01/00843 gcg aag cgt Ala Lys Arg PCT/IBOO/00923 cta gct gca gtg ttg cag gat cct aat Leu Ala Ala Vai Leu Gin Asp Pro Asn ggt Gly ttg gaa ttc Leu Giu Phe acg gtt Thr Val ggt ttc gtg gat Gly Phe Val Asp gtg gtt cga act Val Val Arg Thr gat cgt gaa gcg Asp Arg Giu Ala gca Ala gcg cat gcg ttg Ala His Ala Leu tat Tyr gag ttg ggc aag Giu Leu Gly LYS gct ccg tcg acg Ala Pro Ser Thr tcc ttt ttg gat Ser Phe Leu Asp gcg cag att cag Ala Gin Ile Gin ggt tct ttg gtg Gly Ser Leu Val ggg cgg Gly Arg 100 gcg ttg ccg Ala Leu Pro atg gtt ggg Met Val Gly 120 gtt gtg gtt cct Val Val Val Pro gcg cgg gct cga Ala Arg Ala Arg atc cgg cag Ile Arg Gin 115 ttc gcc aag Phe Ala Lys cac atg att gtg His Met Ile Val gcc cgc gac aag Ala Arg Asp Lys cag Gin 130 gct gtc Ala Val 135 gct gag att cag Ala Ciu Ile Gin tcg Ser 140 gat ggg cac cgc Asp Gly His Arg aac atc aat ttg Asn Ile Asn Leu ggt gaa gcg gtg ttg Gly Glu Ala Val Leu 155 ggc cga aag gag Gly Arg Lys Giu gcg aag cat ttg Ala Lys His Leu gac acg gtg cgg Asp Thr Val Arg ttg cgc cgt ccg Leu Arg Arg Pro gtg gaa tat gtg Val Glu Tyr Val tcc atc Ser Ile 180 aag gtc tct Lys Val Ser acc gtt aat Thr Val Asn 200 gtg gca tcg cag att tcg atg tgg ggt Val Ala Ser Gin Ile Ser Met Trp Gly 190 ttc gaa gac Phe Giu Asp 195 ata gag gcc Ile Glu Ala tat gtt gtg gaa Tyr Val Val Glu ctg aca cct tta Leu Thr Pro Leu gcg cgg Ala Arg 215 gcg ccg aaa ggc Ala Pro Lys Gly acg Thr 220 aag ttc atc aac Lys Phe Ile Asn gac atg gag gaa Asp Met Giu Giu tac Tyr 230 cgc gat ctg cgc Arg Asp Leu Arg act atg gag gtg Thr Met Giu Val aag cgg ctg ctc Lys Arg Leu Leu aat cca gag ctg Asn Pro Glu Leu gaa cta gaa gcc Glu Leu Giu Ala att gtg ttg cag Ile Val Leu Gin gcg tac Ala Tyr 260 ctt ccc gat Leu Pro Asp ctc ggt gca atc Leu Gly Ala Ile gac ttg gcg cag Asp Leu Ala Gin ttc ggc cgc Phe Gly Arg 275 WO 01/00843 gag cgc gtc Glu Arg Val 280 PCTIiBOO/00923 aac aca ggc ggg Asn Thr Gly Gly ggc gtt aag gtt Gly Val Lys Val ggt gct 1027 Gly Ala 295 cca gtt 1075 Pro Vai 310 cgc gtc 1123 Arg Val ctg ggc 1171 Leu Gly ctc tct 1219 Leu Ser cag ggc 1267 Gin Gly 375 gag ctg 1315 Giu Leu 390 gcc att 1363 Ala Ilie aac tt~c 1411 Asn Phe aag cga 1459 Lys Arg ctc atc 1507 Leu Ilie 455 aaa gag 1555 aat ttg cct atg gag Asn Leu Pro Met Giu 300 gcc aca gaa cct tcc Ala Thr Giu Pro Ser 315 ctc tat tgg acg atg Leu Tyr Trp, Thr Met cac gtc cac gcg cag His Vai His Ala Gin 305 aaa caa gcc acc gat Lys Gin Ala Thr Asp cgc ctg gtC aag 979 Arg Leu Vai Lys 290 atc acc ggc tgg Ile Thr Gly Trp, gcc aat tac aag Ala Asn Tyr Lys 325 gag ggc ctg cgc Giu Giy Leu Arg 340 ttc gca cat ttg Phe Ala His Leu cgc aaa gaa Arg Lys Giu 335 330 gtt gcc Val Ala 345 gtg gag Val Giu ggc cac aac ctt ttc Giy His Asn Leu Phe 350 cgt ggg gta gcg gac Arg Giy Val Ala Asp 365 tcc gat cag gcg cgc Ser Asp Gin Ala Arg gac ata gca Asp Ile Aia 355 cgt gtg gag ttc gaa atg ctg Arg Val Giu Phe Giu Met Leu 370 gcc gtc agc gtt gac gtc ggt Ala Val Ser Val Asp Vai Giy gcg Ala 380 385 ctg ctt tac gta Leu Leu Tyr Val 395 tct tac ctc gtg Ser Tyr Leu Val 410 atg tcc gcc atc Met Ser Ala Ile cca gcc gtg cgc Pro Ala Val Arg cgc cgc ctc gag Arg Arg Leu Giu cca Pro 400 caa Gin gaa ttc gac gtg Giu Phe Asp Val 405 ttc gac ctc Phe Asp Leu 415 gac Asp gaa aac gcc gcg agc gaa Giu Asn Ala Ala Ser Giu 420 gcc gac aac ccg tcc ttc Ala Asp Asn Pro Ser Phe 435 425 gag gag Giu Giu agc cgc ttc cgc Ser Arg Phe Arg 445 440 gac Asp 430 gcc tcc ata tct gac ctc gcc acg Ala Ser Ile Ser Asp Leu Ala Thr 450 ccc aac cac aca caa gac cgc agc Pro Asn His Thr Gin Asp Arg Ser gtg ccc gcg ccc Val Pro Ala Pro 460 9gC Gly 465 ccc ctc gtc cca ttt acg ctt ctc gac gcc atc aac gag ccc WO 01/00843 WO 0100843PCTLEBOO/00923 Lys Glu 470 gac acc 1603 Asp Thr gtc gcc 1651 Val Ala gag gtg 1699 Glu Val gac gct 1747 Asp Ala 535 att ttg 1795 Ile Leu 550 atc tca 1843 Ile Ser ccg gaa 1891 Pro Glu gcc ctg 1939 Ala Leu gtC gtt 1987 Val Val 615 gga tcg 2035 Gly Ser 630 ccc tca 2083 Pro Ser tgg gaa 2131 Trp Glu Thr Leu Leu Asp Ala Pro Leu Val Pro Phe Ile Asn Giu Pro aac cca gcg Asn Pro Ala 490 acc gca gca Thr Ala Ala ctc atc caa aac caa Leu Ile Gin Asn Gin 495 gag ccc ggt tgg ttg Glu Pro Giy Trp Leu 505 ttg gaa Leu Glu cag tgg gcc aca aaa gcc Gin Trp Ala Thr Lys Ala 500 gaa aaa caa aca aag ccg Glu Lys Gin Thr Lys Pro 515.

cta att aac gat gtg cgc Leu Ile Asn Asp Val Arg 530 gcc cgt gaa cgc gct gag Ala Arg Glu Arg Ala Glu gag ggg gac Glu Gly Asp gcg tgg gca Ala Trp, Ala gtc gac aag Val Asp Lys 525 gcg cgc cca Ala Arg Pro gaa Glu 540 tac aag acc gcc Tyr Lys Thr Ala 555 gtg acg gcc gcg Val Thr Ala Ala 570 gag Glu gag Glu att ttg cgc gtg Ile Leu Arg Val 560 gtg ggc aaa gct Val Gly Lys Ala 545 cga cgc gga cac ctg Arg Arg Gly His Leu 565 gtg gaa caa acc gac Val Giu Gln Thr Asp 580 575 atc tct Ilie Ser 585 gaa ttg Glu Leu gaa gcc att gat ttc Glu Ala Ilie Asp Phe 590 gac gac gta gac aat Asp Asp Val Asp Asn 605 acc ccg ccc tgg aat Thr Pro Pro Trp Asn gcc cgc tac tac gcg cat ttg Ala Arg Tyr Tyr Ala His Leu 595 gcg gaa ttc acc cca gat cgc Ala Glu Phe Thr Pro Asp Arg 610 ttc ccc atc gcg atc ccc gct Phe Pro Ile Ala Ile Pro Ala 600 gtg Val1 g tg Val 620 act ttc gca Thr Phe Ala aag cct agc Lys Pro Ser 650 gcc ggc gtt Ala Gly Val gca ctc gcg gcg ggc Ala Leu Ala Ala Gly 635 caa cat tgc tcc gct Gin His Cys Ser Ala 655 ccc cgc gag gtt ctg Pro Arg Glu Val Leu 625 gct ggc Ala Gly gtg atc cac aaa Val Ile His Lys 645 gca gtg gtc gaa gcc ctc Ala Val Val Glu Ala Leu 660 cat tgc att tac cca gct His Cys Ile Tyr Pro Ala WO 01/00843 WO 0100843PCTIiBOO/00923 aat cgc 2179 Asn Arg gtc att 2227 Val Ile 695 cga cca 2275 Arg Pro 710 gtg gtc 2323 Val Val aaa tcc 2371 Lys Ser gga tgt gcg Gly Cys Ala ttg acc ggc tcc tcc Leu Thr Gly Ser Ser 700 gaa ctc acc atc aac Glu Leu Thr Ile Asn 715 670 675 ttg atc agc cat gaa cac gtc gac cgc Leu Ile Ser His Giu His Val Asp Arg 685 690 gag acc gcc gcg atg ttc tcc tcc tgg Giu Thr Ala Ala Met Phe Ser Ser Trp 705 ggc gaa acc tcc ggc aaa aac gcc atc dly Glu Thr Ser Gly Lys Asn Ala Ile 720 725 acc cca tct Thr Pro Ser 730 gcc ttc ggc Ala Phe Gly 745 ttg gta ggc Leu Val Gly gcc gac cgc gac ctc gcc Ala Asp Arg Asp Leu Ala 735 cat gca gga caa aaa tgt His Ala Gly Gin Lys Cys gtc gcc gac ctg gtg Val Ala Asp Leu Val 740 tcc gca gcc tcc ctc Ser Ala Ala Ser Leu 755 ggc atc 2419 Gly Ile ctg gta 2467 Leu Val 775 tcc gca 2515 Ser Ala 790 cac gcc 2563 His Ala cga caa 2611 Arg Gin ggc gtc 2659 Gly Val gtC ctc 2707 Val Leu 855 750 agc gtc tac gaa Ser Val Tvr Glu tca gaa cgc ttc cgg aaa cag Ser Glu Arg Phe Arg Lys Gin 770 gtc gac tgg cct acc aac ccc Val Asp Ti-p Pro Thr Asn Pro gcc gca tcc tca Ala Ala Ser Ser 780 atc Ile 785 acc gtc gga cca Thi- Val Gly Pro 795 cta acc acc ctc Leu Thr Thi- Leu 810 ctc gac gac acc Leu Asp Asp Thr ctc acc gaa ctc Leu Thr Giu Leu gaa gaa gga gaa Glu Glu Gly Giu 815 ggc cga ctc tgg Gly Arg Leu Ti-p ccc Pro 800 agc Ser gat aaa ctc cac Asp Lys Leu His 805 agc tgg ctg ctg aaa ccc Ser Trp, Leu Leu Lys Pro 820 tca ccc ggc atc aaa gaa Ser Pro Gly Ile Lys Giu 835 aca gaa gta ttc gga cca Thr Giu Val Phe Gly Pro 825 aaa cca Lys Pro 830 gga acc ttc ttc cac Gly Thr Phe Phe His ctc Leu ctg atg aaa gcc Leu Met Lys Ala 860 850 gac ctc aat gaa gcc Asp Leu Asn Giu Ala 865 atc gaa ttc Ile Glu Phe WO 01/00843 WO 0100843PCTIiBOO/00923 caa aac 2755 Gin Asn 870 gcc gac 2803 Ala Asp tac gtc 2851 Tyr Val gga ggc 2899 Gly Gly ccc aac 2947 Pro Asn 935 cac gcc 2995 His Ala 950 gga gaa 3043 Gly Glu gca tgg 3091 Ala Trp gta gaa 3139 Val Giu ctc gac 3187 Leu Asp 1015 gcc cgt 3235 Ala Arg 1030 tca gag 3283 ggc aac gac Gly Asn Asp gaa gtc cgc Giu Val Arg 890 aac cgc ggc Asn Arg Gly 905 ttc gga ctc acc ggc Phe Giy Leu Thr Gly 875 acc tgg ctt gac cac Thr Trp Leu Asp His 895 atc acc ggc gcc att Ile Thr Gly Ala Ile 910 tcc tcc gtc ggc ctc Ser Ser Val Gly Leu gga ctc caa tcc ctc gac Gly Leu Gin Ser Leu Asp 880 885 gtc gat gtc gga aac gcc Val Asp Val Gly Asn Ala 900 gtc caa cgc caa tcc ttc Val Gin Arg Gin Ser Phe 915 gga tcc aaa gcc gga gga Gly Ser Lys Ala Gly Gly tgg Trp 920 aaa aaa Lys Lys 925 tat gtc atg ctc Tyr Vai Met Leu cca cgc gaa aca Pro Arg Giu Thr atg Met 940 gga Gly acc Thr 930 tgg gcc gac gcg cca agc cac Trp Ala Asp Ala Pro Ser His 945 aac ccg ctg Asn Pro Leu ctc gaa aaa Leu Giu Lys atc agc Ile Ser 960 aaa Lys ctg gat ctc ccc Leu Asp Leu Pro 965 gag ctc gaa Giu Leu Giu 970 aac acg gaa ttc ggc agc cca Asn Thr Giu Phe Gly Ser Pro 985 990 gcc aac att ttc cgc tac cga Ala Asn Ilie Phe Arg Tyr Arg 1000 1005 gat tcc gcc aca ccc cga gaa Asp Ser Ala Thr Pro Arg Giu 1020 cgc gcc ggg gtt act ccg cga Arg Ala Gly Val Thr Pro Arg 1035 caa gtc cgc gaa gta ttg tcc gcc aac gcc agc gat gaa aca Ala Asn Ala Ser Asp Giu Thr 975 980 cgc gac ccc tcc ggc ctc gat Arg Asp Pro Ser Gly Leu Asp 995 cca gca gag gta gta ctc cga Pro Ala Glu Vai Val Leu Arg 1010 act gcc cgc gca ttg ttg gca Thr Ala Arg Ala Leu Leu Ala 1025 gtt ctt caa aca cca ggt gtt Val Leu Gin Thr Pro Gly Val 1040 1045 gct gct gga gtg agt gca gaa Ala Ala Gly Val Ser Ala Glu 1055 1060 Ser Giu Gin Val Arg Giu Val Leu 1050 WO 01/00843 PCTIIBOO/00923 aca gtc gat gat tcg gta ttt att tcc aac gtg ttg cgc ggc gaa tac 3331 Thr Val Asp Asp Ser Val Phe Ile Ser Asn Val Leu Arg Gly Glu Tyr 1065 1070 1075 gac gag aactcc agc gtc cga gtc cgc tac ctg ggc aaa gtt agc gac 3379 Asp Glu Asn Ser Ser Val Arg Val Arg Tyr Leu Gly Lys Val Ser Asp 1080 1085 1090 act gtc cgt gaa cgc cta tct gta cgg ccc gaa gtt gtt ctg ctt gac 3427 Thr Val Arg Glu Arg Leu Ser Val Arg Pro Glu Val Val Leu Leu Asp 1095 1100 1105 gat gca gta act gcc tcc ggt cga gtt gaa tta cgi tac tgg ctc aaa 3475 Asp Ala Val Thr Ala Ser Gly Arg Val Glu Leu Arg Tyr Trp Leu Lys 1110 1115 1120 1125 gaa caa gca att tcc atg acg ttg cac cgt ttt gga aac cca gtt gcg 3523 Giu Gin Ala Ile Ser Met Thr Leu His Arg Phe Gly Asn Pro Val Ala 1130 1135 1140 gcc ttc cac gag ttg gcg gag gaa ctt aaa cgt tgatcgtttt gcgcatgggt 3576 Ala Phe His Giu Leu Ala Glu Glu Leu Lys Arg 1145 1150 cgc 3579 <210> 308 <211> 1152 <212> PRT <213> Corynebacteriumr glutamicun <400> 308 Met Thr Ser Met Asn Leu Pro Ile Glu Leu Ala Thr Leu Ser Asp Gin 1 5 10 Ala Val Asp Lys Val Arg Ser Trp Leu Giu Tyr Ser Lys Lys Glu Ser 25 Val Pro Asn Ala Asp Ala Lys Arg Leu Ala Ala Val Leu Gin Asp Pro 40 Asn Gly Leu Glu Phe Thr Val Gly Phe Val Asp Arg Val Val Arg Thr 55 Glu. Asp Arg Glu Ala Ala Ala His Ala Leu Tyr Glu Leu Gly Lys Ile 70 75 Ala Pro Ser Thr Met Ser Phe Leu Asp Arg Ala Gin Ile Gin Ala Gly 90 Ser Leu Val Gly Arg Ala Leu Pro Gin Val Val Val Pro Ala Ala Arg 100 105 110 WO 01/00843 Ala Arg Ii 11 Lys Gin Ph 130 Leu Asn Ii 145 Ala Lys Hi Giu Tyr Va Trp Gly Ph 19 Leu Tyr Ii 210 Leu Asp Me 225 Lys Arg Le Val Leu Gi Ala Gin Ph 27 Val Arg Le 290 Gin Ile Th 305 Asp Ala As Met Giu Gi Ala Phe Al Glu Phe Gi 370 Ser Val As 385 Gin Glu Ph Asn Ala Al e 5 e e 5 1 5 e n 5

U

r n y a 5 u p e a Arg Ala Asn Leu Ser 180 Giu Glu Giu Leu Ala 260 Gly Val Gly Tyr Leu 340 His Met Val1 Asp Ser 420 Gin Lys Leu Asp 165 Ile Asp Ala Giu Ser 245 Tyr Arg Lys Trp Lys 325 Arg Leu Leu Gly Val 405 Glu Met Ala Leu 150 Asp Lys Thr Ala Tyr 230 Asn Leu Glu Gly Pro 310 Arg Leu Leu Gin Glu 390 Ala Asn Gly 120 Ala Gi u Val1 Ser Asn 200 Ala Asp Glu Asp Val 280 Asn Ala Leu Val Val 360 Met Leu Ser Met His Glu Ala Arg Ser 185 Tyr Pro Leu Leu Ala 265 Asn Leu Thr Tyr Ala 345 Glu Ala Leu Tyr Ser 425 Met Ile Val Leu 170 Val1 Val1 Lys Arg His 250 Leu Thr Pro Giu Trp 330 Gly Arg Ser Tyr Leu 410 Ala Ile Gin Leu 155 Leu Ala Val1 Gly Leu 235 Glu Gly Gly Met Pro 315 Thr His Gly Asp Val 395 Val1 Ile Val1 Ser 140 Gly Arg Ser Glu Thr 220 Thr Leu Ala Gly Glu 300 Ser Me~t Asn Val1 Gin 380 Pro Arg Phe Asp 125 Asp Arg Arg Gin Gin 205 Lys Met Glu Ile Ala 285 His.

Lys Arg Leu Ala 365 Ala Al a Arg Asp PCTLBOO/00923 Arg Asp His Arg Glu Ala 160 Asp Val 175 Ser Met Thr Pro Ile Asn Val Phe 240 Gly Ile 255 Asp Leu Val Lys His Ala Ala Thr 320 Giu Asn 335 Asp Ile Arg Val Ala Val Arg Pro 400 Giu Giu 415 Asp Ala Asp Asn Pro Ser Phe Lys Arg Glu Giu Ser Arg Phe Arg Ala Ser Ile WO 01/00843 PCT/IB00/00923 435 440 445 Ser Asp Leu Ala Thr Leu Ile Asp Val Pro Ala Pro Gly Pro Asn His 450 455 460 Thr Gln Asp Arg Ser Lys Glu Thr Leu Leu Asp Ala Pro Leu Val Pro 465 470 475 480 Phe Ile Asn Glu Pro Asp Thr Asn Pro Ala Leu Ile Gln Asn Gln Gln 485 490 495 Trp Ala Thr Lys Ala Val Ala Thr Ala Ala Glu Pro Gly Trp Leu Glu 500 505 510 Lys Gin Thr Lys Pro Glu Val Leu Glu Glu Gly Asp Val Asp Lys Leu 515 520 525 Ile Asn Asp Val Arg Asp Ala Ala Glu Ala Trp Ala Ala Arg Pro Ala 530 535 540 Arg Glu Arg Ala Glu Ile Leu Tyr Lys Thr Ala Glu Ile Leu Arg Val 545 550 555 560 Arg Arg Gly His Leu Ile Ser Val Thr Ala Ala Glu Val Gly Lys Ala 565 570 575 Val Glu Gin Thr Asp Pro Glu Ile Ser Glu Ala Ile Asp Phe Ala Arg 580 585 590 Tyr Tyr Ala His Leu Ala Leu Glu Leu Asp Asp Val Asp Asn Ala Glu 595 600 605 Phe Thr Pro Asp Arg Val Val Val Val Thr Pro Pro Trp Asn Phe Pro 610 615 620 Ile Ala Ile Pro Ala Gly Ser Thr Phe Ala Ala Leu Ala Ala Gly Ala 625 630 635 640 Gly Val Ile His Lys Pro Ser Lys Pro Ser Gin His Cys Ser Ala Ala 645 650 655 Val Val Glu Ala Leu Trp Glu Ala Gly Val Pro Arg Glu Val Leu His 660 665 670 Cys Ile Tyr Pro Ala Asn Arg Asp Val Gly Cys Ala Leu Ile Ser His 675 680 685 Glu His Val Asp Arg Val Ile Leu Thr Gly Ser Ser Glu Thr Ala Ala 690 695 700 Met Phe Ser Ser Trp Arg Pro Glu Leu Thr Ile Asn Gly Glu Thr Ser 705 710 715 720 Gly Lys Asn Ala Ile Val Val Thr Pro Ser Ala Asp Arg Asp Leu Ala 725 730 735 Val Ala Asp Leu Val Lys Ser Ala Phe Gly His Ala Gly Gin Lys Cys 740 745 750 Ser Ala Ala Ser Leu Gly Ile Leu Val Gly Ser Val Tyr Glu Ser Glu 755 760 765 WO 01/00843 Arg Phe Arg 770 Trp Pro Thr 785 Ser Asp Lys Trp Leu Leu Pro Gly Ilie 835 Glu Val Phe 850 Giu Ala Ilie 865 Leu Gin Ser Asp Val Gly Gin Arg Gin 915 Ser Lys Aia 930 Asp Ala Pro 945 Lys Leu Asp Ala Ser Asp Pro Ser Gly 995 Giu Vai Vai 1010 Arg Ala Leu 1025 Gin Thr Pro Lys Asn Leu Lys 820 Lys Gly Glu Leu Asn 900 S er Gly Ser Leu Giu 980 Leu Leu Leu Giy Gin Leu Vai Asp Ala 775 Pro Ser Ala Thr Val 790 His His Ala Leu Thr 805 Pro Arg Gin Leu Asp 825 Giu Gly Val Lys Pro 840 Pro Val Leu Gly Leu 855 Phe Gin Asn Gly Asn 870 Asp Ala Asp Giu Val 885 Ala Tyr Val Asn Arg 905 Phe Gly Giy Trp Lys 920 Gly Pro Asn Tyr Val 935 His His Ala Pro Arg 950 Pro Gly Giu Glu Leu 965 Thr Ala Trp Asn Thr 985 Asp Val Giu Ala Asn 1000 Arg Leu Asp Asp Ser 1015 Ala Ala Arg Arg Ala 1030 Val Ser Giu Gin Val 1045 Giu Thr Val Asp Asp Ala Ser Ser Leu Ile 780 Gly Pro Leu Thr Giu 795 Thr Leu Giu Giu Gly 810 Asp Thr Gly Arg Leu 830 Gly Thr Phe Phe His 845 Met Lys Ala Thr Asp 860 Asp Phe Giy Leu Thr 875 Arg Thr Tip, Leu Asp 890 Gly Ile Thr Gly Ala 910 Lys Ser Ser Val Gly 925 Met Leu Met Gly Thr 940 Giu Thr Asn Pro Leu 955 Giu Trp Leu Glu Lys 970 Giu Phe Gly Ser Pro 990 Ilie Phe Arg Tyr Arg 1005 Ala Thr Pro Arg Giu 1020 Gly Val Thr Pro Arg 1035 Arg Glu Val Leu Ser 1050 Ser Val Phe Ile Ser PCTJIBOO/00923 Vai Asp Leu Pro 800 Glu Ser 815 Tip Ser Leu Thr Leu Asn Gly Gly 880 His Val 895 Ile Val Leu Giy Tip, Ala Ile Ser 960 Ala Asn 975 Arg Asp Pro Ala Thr Aia Val Leu 1040 Ala Ala 1055 Asn Val Giy Val Ser Ala 1060 Leu Arg Gly Giu 1075 1065 1070 ryr Asp Giu Asn Ser Ser Val Arg Val Arg Tyr Leu 1080 1085 WO 01/00843 PCT/1B00100923 Gly Lys Val Ser Asp Thr Val Arg Glu Arg Leu Ser Val Arg Pro Glu 1090 1095 1100 Vai Val Leu Leu Asp Asp Ala Val Thr Ala Ser Gly Arg Val Glu Leu 1105 1110 1115 1120 Arg Tyr Trp Leu Lys Glu Gin Ala Ilie Ser Met Thr Leu His Arg Phe 1125 1130 1135 Gly Asn Pro Val Ala Ala Phe His Glu Leu Ala Glu Glu Leu Lys Arg 1140 1145 1150 <210> 309 <211> 476 <212> DNA <213> Corynebacterium glutarnicum <220> <221> CDS <222> (453) <223> FRXA00023 <400> 309 cat ttt ccg ctc His Phe Pro Leu 1 gcc aca ccc cga Ala Thr Pro Arg ggg gtt act ccg Gly Val Thr Pro cgc gaa gta ttg Arg Glu Val Leu tcg gta ttt att Ser Val Phe Ilie agc gtc cga gtc Ser Val Ara Val cga Arg gca Ala gtt Val1 gaa Glu tac Tyr gac Asp gac Asp aaa Lys gcg c tc Leu gcc Ala tca Ser aca Thr gat tcc Asp Ser cgc gcc Arg Ala caa gtc Gin Val gat gat Asp Asp gac gag aac Asp Glu Asn cgc Arg gcc Ala tcc gta Val1 100 cga Arg ttg act Thr gat Asp gaa Giu 125 gcc Ser Met Thr Leu His Arg Phe Gly Asn Pro Val Ala Ala Phe His. Giu WO 01/00843 PCTI/iBOO/00923 130 135 140 ttg gcg gag gaa ctt aaa cgt tgatcgtttt gcgcatgggt cgc 476 Leu Ala Giu Giu Leu Lys Arg 145 150 <210> 310 <211> 151 <212> PRT <213> Corynebacterium glutarnicum <400> 310 His Phe Pro Leu Arg Pro Ala Glu Val Val Leu Arg Leu Asp Asp Ser 1 5 10 Ala Thr Pro Arg Glu Thr Ala Arg Ala Leu Leu Ala Ala Arg Arg Ala 25 Gly Val Thr Pro Arg Val Leu Gin Thr Pro Gly Val Ser Glu Gin Val 40 Arg Glu Val Leu Ser Ala Ala Gly Val Ser Ala Glu Thr Val Asp Asp 55 Ser Val Phe Ile Ser Asn Val Leu Arg Gly Glu Tyr Asp Glu Asn Ser 70 75 Ser Val Arg Val Arg Tyr Leu Gly Lys Val Ser Asp Thr Val Arg Glu 90 Arg Leu Ser Val Arg Pro Glu Val Val Leu Leu Asp Asp Ala Val Thr 100 105 110 Ala Ser Gly Arg Val Glu Leu Arg Tyr Trp Leu Lys Glu Gin Ala Ile 115 120 125 Ser Met Thr Leu His Arg Phe Gly Asn Pro Val Ala Ala Phe His Glu 130 135 140 Leu Ala Glu Glu Leu Lys Arg 145 150 <210> 311 <211> 3124 <212> DNA <213> Corynebacterium giutamicum <2 <221> CDS <222> (101)..(3124) <223> FRXA02284 <400> 311 gggattatgt tgggggccac atctgaaaat agttgctggg atccatcaca cctacgatgc gaaacggcac ccacaccgct gatcttgaag gagaaccacc atg acg tcg atg aat 115 Met Thr Ser Met Asn 1 WO 01/00843 ctg cct att Leu Pro Ile cgc tcc tgg Arg Ser Trp gcg aag cgt Ala Lys Arg acg gtt ggt Thr Val Gly gca gcg cat Ala Ala His tcc ttt ttg Ser Phe Leu gcg ttg ccg Ala Leu Pro atg gtt ggg Met Val Gly 120 gct gtc gct Ala Val Ala 135 cta ggt gaa Leu dly Glu 150 gac acg gtg Asp Thr Val nnn ntc tct Xaa Xaa Ser acc gtt aat Thr Val Asn 200 gcg cgg gcg Ala Arg Ala 215 tac cgc gat Tyr Arg Asp 230 gct Ala tac Tyr gca Ala ga t Asp tat Tyr 75 gcg Ala gtg Val1 att Ile cag Gin ttg Leu 155 t tg Leu gca Ala gtg Val ggc Gly ctg Leu 235 acg Thr agc Ser gtg Val1 cga Arg gag Glu cag Gin gtt Val1 gtg Val1 tcg Ser 140 ggc Gly cgc Arg tcg Ser gaa Glu acg Thr 220 act Thr ctg Leu aaa Lys Ctg Leu gtg Val ttg Leu att Ile cc t Pro gat Asp 125 gat Asp cga Arg cgt Arg cag Gin cag Gin 205 aag Lys atg Met t ct Ser aag Lys cag Gin gtt Val1 Gly cag Gin gcg Ala 110 gcc Ala ggg Gly aag

LYS

ccg Pro att Ile 190 Leu ttc Phe gag Glu gac Asp gaa Glu gat Asp cga Arg aag Lys gcc Ala gcg Ala cgc Arg c ac His gaa Glu gat Asp 175 tcg Ser aca Thr atc Ile gtg Val gac Asp aat Asn t tg Leu cgt Arg tcg Ser gtg Val atc Ile 115 ttc Phe atc Ile cat His gtg Val1 t tc Phe 195 ata Ile atg Met ctg Leu PCTI.IBOO/00923 aag gtg 163 Lys Val gcc gat 211 Ala Asp gaa ttc 259 Giu Phe gaa gcg 307 Glu Ala acg atg 355 Thr Met ggg Cgg 403 Gly Arg 100 cgg cag 451 Arg Gin gcc aag. 499 Ala Lys aat ttg 547 Asn Leu ttg gat 59*5 Leu Asp 165 tcn r=n 643 Ser Xaa 180 gaa gac 691 Giu Asp gag ccc 739 Giu Pro gag gaa 787 Giu Glu ctc tcc 835 Leu Ser 245 aat cca gag ctg cat gaa cta gaa gcc gga att gtg ttg cag gcg tac WO 01/00843 WO 0100843PCTJIBOO/00923 Asn Pro Giu Leu His 250 otc Leu Glu Leu Giu Ala Gly 255 gac Asp Ile Val Leu Gin Ala Tyr 260 ott ccc gat Leu Pro Asp gag cgc gtc Giu Arg Val 280 gcc Ala 265 aac Asn ggt gca atc Giy Ala Ile ttg gcg cag Leu Ala Gin tto ggc cgc Phe Gly Arg 275 931 979 aca ggc ggg Thr Gly Giy gog Ala 285 cac His ggt got 1027 Gly Ala 295 cca gtt 1075 Pro Val 310 ogc gtc 1123 Arg Val ctg ggc 1171 Leu Gly oto tot 1219 Leu Ser cag ggc 1267 Gin Gly 375 gag ctg 1315 Glu Leu 390 gcc att 1363 Ala Ile aac ttc 1411 Asn Phe aag cga 1459 aat ttg oct atg Asn Leu Pro Met goc aca gaa cot Ala Thr Giu Pro 315 ctc tat tgg acg Leu Tyr Trp Thr 330 gtt gcc ggc cac Val Ala Gly His ggc gtt aag gtt Gly Val Lys Val gtc cac gcg cag Val His Ala Gin 305 cgc ctg gtc aag Arg Leu Val Lys 290 ato acc ggc tgg Ile Thr Gly Trp gcc aat tao aag Ala Asn Tyr Lys 325 toc aaa caa gcc acc Ser Lys Gin Ala Thr 320 atg cgc aaa gaa aac Met Arg Lys Glu Asn 335 aac ott ttc gac ata Asn Leu Phe Asp Ile gat Asp atg gag ggc ctg cgc Met Giu Gly Leu Arg 340 gca ttc gca cat ttg Ala Phe Ala His Leu 355 345 gtg gag Val Glu cgt ggg gta gcg Arg Gly Val Ala 365 tcc gat cag gcg Ser Asp Gin Ala 350 gac cgt gtg gag tto gaa atg ctg ASP Arg Val Giu Phe Glu Met Leu 370 cgc gcc gto agc gtt gac gto ggt Arg Ala Val Ser Val Asp Val Gly 385 gcg 380 ctg ctt tac gta Leu Leu Tyr Val 395 tot tac oto gtg Ser Tyr Leu Val 410 atg toc gcc atc Met Ser Ala Ilie cca gcc gtg cgc cca Pro Ala Val Arg Pro 400 cgc cgc oto gag gaa Arg Arg Leu Giu Glu caa gaa ttc gac gtg Gin Giu Phe ASP Val 405 aac gcc gcg agc gaa Asn Ala Ala Ser Giu 420 gac aac ccg too ttc Asp Asn Pro Ser Phe 435 tot gac oto gcc acg Ser Asp Leu Ala Thr 450 425 gag gag ago cgo ttc gac oto Phe Asp Leu 430 tto ogc gcc Phe Arci Ala tcc ata Ser Ile Lys Arg Giu Glu Ser Arg 440 445 WO 01/00843 WO 0100843PCT/IBOO/00923 ctc atc gac gtg ccc gcg ccc ggc ccc aac cac aca caa gac cgc agc 1507Pr Leu Ile Asp 455.

aaa gag acg 1555 Lys Giu Thr 470 gac acc aac 1603 Asp Thr Asn Val Pro Ala ctt ctc gac Leu Leu Asp 475 cca gcg ctc Pro Ala Leu 460 gcc Ala atc Ile Gly Pro Asn His ccc ctc gtc cca Pro Leu Val Pro 480 caa aac caa cag Gin Asn Gin Gin Gin Asp Arg Ser atc aac gag ccc Ile Asn Giu Pro 485 gcc aca. aaa gcc tgg 490 gtc gcc 1651 Val Ala gag gtg i6 99 Glu Val gac gct 1747 Asp Ala 535 att ttg 1795 Ile Leu 550 atc tca 1843 Ile Ser ccg gaa 1891 Pro Giu gcc ctg 1939 Ala Leu gtC gtt 1987 Val Val 615 acc gca Thr Ala 505 gca Ala gag ccc ggt tgg Glu Pro Gly Trp 495 500 ttg Leu gaa gag ggg gac gtc Giu Giu*Gly Asp Val 525 gaa gcg tgg gca gcg Giu Ala Trp Ala Ala 510 gac Asp cgc Arg ttg gaa aaa caa aca. aag ccg Leu Giu Lys Gin Thr Lys Pro 515 aag cta att aac gat gtg cgc Lys Leu Ile Asn Asp Val Arg 530 cca gcc cgt gaa cgc gct gag Pro Ala Ar g Giu Arg Ala Giu 545 tac aag acc gcc Tyr Lys Thr Ala 555 gtg acg gcc gcg Val Thr Ala Ala 570 att ttg cgc gtg Ile Leu Arg Val 560 cga Arg cgc gga cac ctg Arg Gly His Leu 565 gag gtg ggc aaa Giu Val Gly Lys 575 gct gtg gaa caa acc gac Ala Val Giu Gin Thr Asp 580 cgc tac tac gcg cat ttg Arg Tyr Tyr Ala His Leu 595 atc tct Ile Ser 585 gaa ttg Glu Leu gaa gcc att gat Giu Ala Ile Asp gac gac gta gac Asp Asp Val Asp 605 acc ccg ccc tgg Thr Pro Pro Trp t tc Phe 590 600 gcc Ala aat gcg gaa ttc acc cca gat cgc Asn Ala Giu Phe Thr Pro Asp Arg 610 aat ttc ccc atc gcg atc ccc gct Asn Phe Pro Ile Ala Ile Pro Ala 625 gtg gtg Val Val 620 act ttc gca gca ctc gga tcg 2035 gcg gcg ggc gct ggc gtg atc cac aaa Gly Ser 630 Thr Phe Ala Ala Leu Ala Ala Gly Ala Gly Val Ile His Lys 635 640 645 WO 01/00843 WO 0100843PCT/IBOO/00923 ccc tca 2083 Pro Ser tgg gaa 2131 Trp Giu aat cgc 2179 Asn Arg gtc att 2227 Val Ile 695 cga cca 2275 Arg Pro 710 gtg gtc 2323 Val Val aaa tcc 2371 Lys Ser ggc atc 2419 Gly Ilie ctg gta 2467 Leu Val 775 tcc gca 2515 Ser Ala 790 cac gcc 2563 His Ala cga caa 2611 Arg Gin aag cct agc Lys Pro Ser 650 caa cat tgc tcc gct Gin His Cys Ser Ala 655 gca gtg gtc gaa gcc ctc Ala Val Val Giu Ala Leu 660 cat tgc att tac cca gct His Cys Ile Tyr Pro Ala 675 gcc ggc Ala Gly 665 gat gtt Asp Val gtt ccc cgc gag Val Pro Arg Glu gga tgt gcg ttg Gly Cys Ala Leu gtt Val 670 ctg Leu 680 ttg Leu gaa atc agc cat gaa cac gtc gac cgc Ile Ser His Glu His Val Asp Arg 690 acc gcc gcg atg ttc tcc tcc tgg Thr Ala Ala Met Phe Ser Ser Trp 705 acc ggc tcc Thr Gly Ser ctc acc atc Leu Thr Ile tcc Ser 700 715 acc cca tct gcc Thr Pro Ser Ala 730 gcc ttc ggc cat Ala Phe Gly His 745 ttg gta ggc agc Leu Val Gly Ser 760 gac gcc gca tcc Asp Ala Ala Ser acc gtc gga cca Thr Val Gly Pro 795 cta acc acc ctc Leu Thr Thr Leu 810 ctc gac gac acc Leu Asp Asp Thr 825 aac ggc gaa acc tcc Asn Gly Glu Thr Ser 720 gac cgc gac ctc gcc Asp Arg Asp Leu Ala 735 gca gga caa aaa tgt Ala Gly Gin Lys Cys ggc aaa aac gcc atc Gly Lys Asn Ala Ile 725 gtc gcc gac ctg gtg Val Ala Asp Leu Val 740 tcc gca gcc tcc ctc Ser Ala Ala Ser Leu 750 gtc tac Val Tyr 765 tca ctc Ser Leu 780 gaa Clu tca Ser 755 gaa cgc ttc cgg aaa cag Glu Arg Phe Arg Lys Gin 770 atc gtc gac tgg cct acc aac ccc Ile Val Asp Trp Pro Thr Asn Pro 785 ctc Leu acc gaa ctc Thr Glu Leu gaa gaa gga gaa Giu Glu Gly Glu 815 ggc cga ctc tgg Gly Arg Leu Trp 830 ccc agc gat aaa ctc cac Pro Ser Asp Lys Leu His 800 805 agc tgg ctg ctg aaa ccc Ser Trp Leu Leu Lys Pro 820 tca ccc ggc atc aaa gaa Ser Pro Gly Ile Lys Glu 835 WO 01/00843 WO 0100843PCTIiBOO/00923 ggc gtc 2659 Gly Val gtc ctc 2707 Val Leu 855 caa aac 2755 Gin Asn 870 gcc gac 2803 Ala Asp tac gtc 2851 Tyr Val gga ggc 2899 Gly Giy ccc aac 2947 Pro Asn 935 cac gcc 2995 His Ala 950 gga gaa 3043 Gly Giu gca tgg 3091 Ala Trp aaa Lys 840 cca gga acc ttc Pro Gly Thr Phe ttc cac ctc aca gaa gta ttc gga cca Phe His Leu Thr Giu Val Phe Giy Pro 845 850 acc gac ctc aat gaa gcc atc gaa ttc Thr Asp Leu Asn Giu Ala Ile Giu Phe 865 ggc ctg atg aaa Gly Leu Met Lys ggc aac gac ttc Gly Asn Asp Phe 875 gaa gtc cgc acc Giu Val Arg Thr 890 aac cgc ggc atc Asn Arg Giy Ilie gga ctc acc ggc gga Gly Leu Thr Giy Giy 880 tgg ctt gac cac gtc Trp Leu Asp His Val 895 ctc caa tcc ctc gac Leu Gin Ser Leu Asp 885 gat gtc gga aac gcc Asp Val Gly Asn Ala 900 caa cgc caa tcc ttc Gin Arg Gin Ser Phe acc ggc gcc Thr Gly Ala att Ile gtc Val 905 tgg aaa Trp Lys 910 aaa tcc tcc gtc ggc Lys Ser Ser Val Gly 925 915 ctc gga tcc aaa gcc-gga gga Leu Giy Ser Lys Ala Giy Giy gtc Val1 atg ctc atg Met Leu Met 940 cca cgc gaa aca Pro Arg Giu Thr 955 gag ctc gaa tgg Giu Leu Giu Trp aac Asn .ctc gga acc tgg Giy Thr Trp, ccg ctg atc Pro Leu Ile gaa aaa gcc Glu Lys Ala aac acg Asn Thr 985 gcc aac gcc gac Ala Asp 945 930 gcg cca agc cac Ala Pro Ser His 970 975 agc aaa ctg gat ctc ccc Ser Lys Leu Asp Leu Pro 960 965 aac gcc agc gat gaa aca Asn Ala Ser Asp Giu Thr 980 gac ccc tcc ggc ctc gat Asp Pro Ser Gly Leu Asp 995 gca gaa ttc ggc agc cca cgc Giu Phe Gly Ser Pro Arg 990 att ttc cgc tac cga cca gta gaa 3124 Val Giu Ala Asn Ile Phe Arg Tyr Arg Pro Ala 1000 1005 <210> 312 <211> 1008 <212> PRT <213> Corynebacterium giutamicum WO 01/00843 PCTII1B00I00923 <400> 312 Met Thr Ser Met Asn Leu Pro Ile Giu Leu Ala Thr Leu Ser Asp Gin 1 5 10 Ala Val Asp Lys Val Arg Ser Trp, Leu Glu Tyr Ser Lys Lys Giu Ser 25 Val Pro Asn Ala Asp Ala Lys Arg Leu Ala Ala Vai Leu Gin Asp Pro 40 Asn Gly Leu Giu Phe Thr Val Gly Phe Val Asp Arg Vai Val Arg Thr 55 Giu Asp Arg Giu Ala Ala Ala His Ala Leu Tyr Giu Leu Gly Lys Ile 70 75 Ala Pro Ser Thr Met Ser Phe Leu Asp Arg Ala Gin Ile Gin Ala Giy 90 Ser Leu Val Gly Arg Ala Leu Pro Gin Val Val Val Pro Aia Ala Arg 100 105 110 Ala Arg Ile Arg Gin Met Vai Gly His Met Ile Val Asp Ala Arg Asp 115 120 125 Lys Gin Phe Ala Lys Ala Val Ala Giu Ile Gin Ser Asp Giy His Arg 130 135 140 Leu Asn Ilie Asn Leu Leu Giy Glu Ala Val Leu Gly Arg Lys Giu Ala 145 150 155 160 Ala Lys His Leu Asp Asp Thr Val Arg Leu Leu Arg Arg Pro Asp Vai 165 170 175 Glu Tyr Val Ser xaa Xaa Xaa Ser Ser Val Ala Ser Gin Ile Ser Met 180 185 190 Trp Gly Phe Giu Asp Thr Vai Asn Tyr Val Vai Giu Gin Leu Thr Pro 195 200 205 Leu Tyr Ile.Giu Pro Aia Arg Ala Pro Lys Gly Thr Lys Phe Ile Asn 210 215 220 Leu Asp Met Giu Giu Tyr Arg Asp Leu Arg Leu Thr Met Glu Val Phe 225 230 235 240 Lys Arg Leu Leu Ser Asn Pro Glu Leu His Giu Leu Giu Ala Giy Ile 245 250 255 Vai Leu Gin Ala Tyr Leu Pro Asp Ala Leu Gly Ala Ile Gin Asp Leu 260 265 270 Ala Gin Phe Gly Arg GlU Arg Val Asn Thr Gly Giy Ala Gly Vai Lys 275 280 285 Vai Arg Leu Val Lys Gly Ala Asn Leu Pro Met Glu His Val His Ala 290 295 300 Gin Ilie Thr Gly Trp Pro Vai Ala Thr Glu Pro Ser Lys Gin Ala Thr 305 310 315 320 WO 01/00843 Asp Ala Asn Met Glu Gly Ala Phe Ala 355 Giu Phe Giu 370 Ser Val Asp 385 Gin Giu Phe Asn Ala Ala Asp Asn Pro 435 Ser Asp Leu 450 Thr Gin Asp 465 Phe Ilie Asn Trp Ala Thr Lys Gin Thr 515 Ile Asn Asp 530 Arg Glu Arg 545 Arg Arg Gly Val Glu Gin Tyr Tyr Ala 595 Phe Thr Pro 610 Ile Ala Ile 625 Tyr Leu 340 His Met Val1 Asp Ser 420 Ser Aia Arg Giu Lys 500 Lys Val Aia His Thr 580 His Asp Pro Lys 325 Arg Leu Leu Giy Vali 405 Giu Phe Thr Ser Pro 485 Aia Pro Arg Giu Leu 565 Asp Leu Arg Ala Arg Leu Leu Gin Giu 390 Ala Asn Lys Leu Lys 470 Asp Val Giu Asp Ile 550 Ile Pro Ala Vai Gly 630 Tyr Ala 345 Giu Ala Leu Tyr Ser 425 Giu Val1 Leu Pro Ala 505 Giu Giu Lys Thr Ser 585 Leu Val Phe Trp 330 Gly Arg Ser Tyr Leu 410 Ala Ser Pro Leu Ala 490 Ala Giu Ala Thr Al a 570 Glu Asp Thr Al a Thr His Gly Asp Val 395 Val1 Ile Arg Ala Asp 475 Leu Giu Gly Trp Ala 555 Ala Ala Asp Pro Ala 635 Met Asn Val1 Gin 380 Pro Arg Phe Phe Pro 460 Ala Ile Pro Asp Ala 540 Giu Giu Ile Val Pro 620 Leu Arg Leu Ala 365 Ala Ala Arg Asp Arg 445 Gly Pro Gin Gly Val 525 Ala Ile Val Asp Asp 605 Trp Ala PCT/IB00100923 Giu Asn 335 Asp Ile Arg Val Ala Val Arg Pro 400 Giu Glu 415 Asp Ala Ser le Asn His Val Pro 480 Gin Gin 495 Leu Giu Lys Leu Pro Ala Arg Val 560 Lys Ala 575 Ala Arg Ala Giu Phe Pro Gly Ala 640 Gly Val Ilie His Lys Pro Ser Lys Pro Ser Gin His Cys Ser Ala Ala WO 01/00843 WO 0100843PCTIBOO/00923 Val Vai Giu Ala Leu Trp Giu Ala Gly Val Pro Arg Giu Val Leu His Cys Glu Met 705 Gly Val Ser Arg Trp 785 Ser Trp Pro Glu Giu 865 Leu Asp Gin Ser Asp 945 Lys Ile His 690 Phe Lys Ala Ala Phe.

770 Pro Asp Leu Gly Vai 850 Ala Gin Val Arg Lys 930 Ala Leu Tyr 675 Vai Ser Asn Asp Ala 755 Arg Thr

LYS

Leu Ile 835 Phe Ile Ser dly Gin 915 Ala Pro Asp 660 Pro Asp Ser Ala Leu 740 S er Lys Asn Leu Lys 820 Lys Gly Giu Leu Asn 900 Ser Gly Ser Leu Ala Arg Trp Ile 725 Val1 Leu Gin Pro His 805 Pro Glu Pro Phe Asp 885 Ala Phe Gly His Pro 965 Asp 680 Leu Glu Thr Ala Leu 760 Asp Thr Leu Leu Lys 840 Gly Gly Giu Asn Tip 920 Tyr Pro Glu Ala Ser 700 Asn Asp Ala Val Ser 780 Leu Glu Gly Phe Ala 860 Gly Trp Thr Ser Met 940 Asn Glu Trp Leu Giu Lys Ala Asn WO 01/00843 WO 0100843PCTIIBOO/00923 Ala Ser Asp Glu Thr Ala Trp Asn Thr Giu Phe Gly Ser Pro Arg Asp 980 985 990 Pro Ser Gly Leu Asp Val Glu Ala Asn Ile Phe Arg Tyr Arg Pro Ala 995 1000 1005 <210> 313 <211> 927 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (904) <223> RXC02498 <400> 313 caaggtagaa atttgtccgt gggcacttcg tgaaggtgtg atcctcacca ggatcgacaa aggactcgag taacatttac ccggaaagga gttggcgaaa atg agt gaa gag aaa Met Ser Glu Glu Lys C tC Leu acc Thr gta Val1 ccc Pro cc t Pro gtg Val1 ccg Pro aaa Lys aca Thr gat Asp tcc Ser gcg Ala gag Glu gct Ala gcg Ala gat Asp gtc Val gc t Ala gtt Val1 gag Glu gt t Val1 tcg Ser ccg Pro cca Pro 120 gc t Ala ccc Pro gc t Ala tcc Ser gt t Val gtg Val1 agc Ser 105 att Ile gag Glu cga Arg gag Glu aag Lys gag Glu gag Giu aac Asn agc Ser ctg Leu cga Arg ctg Leu cac His gcc Ala 75 ggc Gly gaa Glu t tg Leu gcc Ala 15 cgc Arg att Ile ccc Pro gag Glu aag Lys gtg Val gcg Ala gcg Al a agc Ser cct Pro atc Ile ccc Pro cag Gin ctg Leu ttc Phe 1 aaa

LYS

atc Ile gcc Ala gat Asp gcc Ala gaa Giu cgc Arg ccc Pro gag gga Glu Gly gaa gac Giu Asp gtt aag Val Lys gca cca Ala Pro gaa gaa Giu Glu acc cct Thr Pro atc gtg Ile Val 115 gtg gtt Val Val 130 gtt gcc gcc aag ccg gcg ccc gta gtg cgc gcg gag aag gac gcc gat WO 01/00843 PCTAIBOO/00923 Lys Pro Ala Pro Val Val Arg Ala Glu Lys Asp Ala Asp Val gtg Val 150 act Thr gag Glu gtc Val1 tgg Trp acc Thr 230 ggt Gly ggg Gly Ala 135 gag Giu gac Asp caa Gin gga Gly gag Giu 215 t tg Leu t tc Phe ccg Pro 140 145 gcc Ala acg Thr aac Asn 185 gtt Val ctg Leu atg Met atg Met gca Ala 265 gta Val cag Gin 170 aaa Lys ctc Leu aac Asn gtg Val gtt Val 250 aag Lys 155 atg met atg Met gg t Gly aag Lys ggc Gly 235 ctc Leu gat ttc gca Asp Phe Ala gtg gtg gaa Val Val Glu 175 gta ttc gcg Val Phe Ala 190 gtt gta ttc Val Val Phe 205 atc gtc gct Ile Val Ala atc cac gct Ile His Ala gga atc gtg Gly Ile Val gaa Giu gac Asp atg Met ttt Phe 210 gca Ala acc Thr gtC gtc Val gag Glu atg Met 195 gaa Giu gtC Val tca Ser atg Leu Val Met Thr Phe 260 atc gtc atg Ile Val Met taatttgtcg ttttgggccc ccg <210> 314 <211> 268 <212> PRT <213> Corynebacteriuni giutamicum <400> 314 Met Ser Giu Glu Lys Leu Thr Val Ala 1 Lys. Giu Gly Arg Ser Thr Asp Ala Pro 25 Ile Glu Asp Gly Gly Val Ser Val Ala 40 Ala Val Lys Giu Lys Pro Ala Giu Ser 55 Asp Ala Pro Ala Glu Pro Giu Vai Val Ala Giu Giu Val Giu Val Ala Ser Val Giu Thr Pro Glu Arg Pro Ala Pro Ser Met Arg Thr S er Pro Asp Giu Ala Arg Gly Ser Lys Val Thr Arg Arg Ser Val Pro Asp Met WO 01/00843 WO 0100843PCT/EBOO/00923 Arg Pro Giu :145 Val1 Val1 Ile Gly Leu 225 Arg Leu Ile Val1 130 Lys Glu Asp Met Phe 210 Ala Thr Val1 Val 115 Val Asp Val1 Glu Met 195 Glu Val1 Ser Met 100 Asp Pro Ala Asp Giu 180 Ala Met Gly Arg Thr 260 Glu Ala Asp Asn 165 Pro Ile Leu Val1 Asp 245 Phe Pro 120 Ala Thr Thr Giu Val1 200 Arg Gly Ser Leu 105 Ile Lys Ala Thr Asn 185 Val Leu Met Met Ala 265 Ser Pro Val1 Gin 170 Lys Leu Asn Val1 Val1 250 Ile Thr Pro 140 Ala Ala Ser Val1 Trp 220 Ile Ala Met Phe Ala Glu 160 Glu Ile Leu Val1 Leu 240 Gly <210> 315 <211> 774 <212> DNA <213> Corynebacteriun giutanicun <2 <221> CDS <222> (101)..(751) <223> RXA01491 <400> 315 aacggtgtga aacacattga agtgtcgttg ggttatccgc gggcagcatg tgtggccata tccagtgatg gaggtggaca ttg ttt cca Leu Phe Pro gtt aat tta Val Asn Leu gta gcg cac Val Ala His aat tcg gca aag ttt Asn Ser Ala Lys Phe gac cat ttc cat cag Asp His Phe His Gin tcg gtt gat att aga Ser Val Asp Ile Arg 45 tct ttc att Ser Phe Ile 15 ttg cat ccg Leu His Pro 30 aaa gca gag Lys Ala Giu gcgaatggga gcattggtct atg ctg gat gag tct Met Leu Asp Giu Ser 1 aaa act ggc gat gct Lys Thr Gly Asp Ala ttg gaa aag gca ctg Leu Glu Lys Ala Leu ttt gga gat gcc agg Phe Gly Asp Ala Arg WO 01/00843 WO 0100843PCT/IBOO/00923 tgg tgt gca cat cag gca ctc caa gct ttg gga cga gat agc ggt gat 307 Trp Cys Ala His Gin Ala Leu Gin Ala Leu Gly Arg Asp Ser Gly Asp 60 ccc att ttg cgt ggg gaa cga gga atg cca ttg tgg cct tct tcg gtg 355 Pro Ile Leu Arg Gly Glu Arg Gly Met Pro Leu Trp Pro Ser Ser Val 75 80 tct ggt tca ttg acc cac act gac gga ttc cga gct gct gtt gtg gcg 403 Ser Giy Ser Leu Thr His Thr Asp Giy Phe Arg Ala Ala Val Val Ala 95 100 cca cga ttg ttg gtg cgt tct atg gga ttg gat gcc gaa cct gcg gag 451 Pro Arg Leu Leu Val Arg Ser Met Gly Leu Asp Ala Giu Pro Ala Giu 105 110 115 ccg ttg ccc aag gat gtt ttg ggt tca atc gct cgg gtg ggg gag att 499 Pro Leu Pro Lys Asp Val Leu Gly Ser Ile Ala Arg Val Gly Giu Ile 120 125 130 cct caa ctt aag cgc ttg gag gaa caa ggt gtg cac tgc gcg gat cgc 547 Pro Gin Leu Lys Arg Leu Giu Glu Gin Gly Val His Cys Ala Asp Arg 135 140 145 ctg ctg ttt tgt gcc aag gaa gca aca tac aaa gcg tgg ttc ccg ctg 595 Leu Leu Phe Cys Ala Lys Giu Ala Thr Tyr Lys Ala Ti-p Phe Pro Leu 150 155 160 165 acg cat agg tgg ctt ggt ttt gaa caa gct gag atc gac ttg cgt gat 643 Thr His Arg Trp Leu Gly Phe Glu Gin Ala Glu Ile Asp Leu Arg Asp 170 175 180 gat ggc act ttt gtg tcc tat ttg ctg gtt cga cca act cca gtg ccg 691 Asp Gly Thr Phe Val Ser Tyr Leu Leu Val Arg Pro Thr Pro Val Pro 185 190 195 ttt att tca ggt aaa tgg gta ctg cgt gat ggt tat gtc ata gct gcg 739 Phe Ile Ser Gly Lys Trp Vai Leu Arg Asp Gly Tyr Val Ile Ala Ala 200 205 210 act gca gtg act tgaactggat ggagaggata cct 774 Thr Ala Val Thr 215 <210> 316 <211> 217 <212> PRT <213> Corynebacteriun giutanicun <400> 316 Met Leu Asp Giu Ser Leu Phe Pro Asn Ser Ala Lys Phe Ser Phe Ile 1 5 10 Lys Thr Gly Asp Ala Val Asn Leu Asp His Phe His Gin Leu His Pro 25 Leu Glu Lys Ala Leu Val Ala His Ser Val Asp Ile Arg Lys Ala Giu 40 WO 01/00843 Phe Gly Asp Ala Arg Trp Cys Ala His Gin Ala 55 Arg Asp Ser Gly Asp Pro Ile Leu Arg Giy Giu 70 75 Trp Pro Ser Ser Val Ser Giy Ser Leu Thr His 90 Ala Ala Val Val Ala Pro Arg Leu Leu Val Arg 100 105 Ala Giu Pro Ala Giu Pro Leu Pro Lys Asp Val 115 120 Arg Val Gly Giu Ile Pro Gin Leu Lys Arg Leu 130 135 His Cys Ala Asp Arg Leu Leu Phe Cys Ala Lys 145 150 155 Ala Trp Phe Pro Leu Thr His Arg Trp Leu Gly 165 170 Ile Asp Leu Arg Asp Asp Gly Thr Phe Val Ser 180 .185 Pro Thr Pro Val Pro Phe Ile Ser Gly Lys Trp 195 200 Tyr Val Ilie Ala Ala Thr Ala Val Thr 210 215 <210> 317 <211> 1287 <212> DNA <213> Corynebacterium glutanicun <22 0> <221> CDS <222> (1264) <223> RXA02155 <400> 317 tcggcgtcgc accttaaagt agcgccttaa agcggcgctt gcaaacacaa caaacacatc taattcagta ggagttccac att acc gcg ccg aaa ggc ttc gtt gct tct gca Ilie Thr Ala Pro Lys Gly Phe Val Ala Ser Ala 15 aaa gct tct ggc aat cct gac atg gcg ttg gtg Lys Ala Ser Gly Asn Pro Asp Met Ala Leu Val 30 gag ttt tcc gca gcg gcc gtg ttt aca cgt aac Giu Phe Ser Ala Ala Ala Val Phe Thr Arg Asn Leu Arg Thr Ser Leu Glu 140 Giu Phe Tyr Val Gin Gly Asp Met Gly 125 Giu Ala Glu Leu Leu 205 PCTIIBOO/00923 Leu Gly Pro Leu Phe Arg Leu Asp Ile Ala Gly Val Tyr Lys 160 Ala Giu 175 Val Arg Asp Gly caaaccaagc gccctaacca atg gca gaa aaa ggc 115 Met Ala Giu Lys Gly 1 acg acc gcg ggt att 163 Thr Thr Ala Gly Ile gtt aac cag ggt cca 211 Val Asn Gin Gly Pro cga gtt ttc gca gcg 259 Arg Val Phe Ala Ala WO 01/00843 WO 0100843PCTJIBOO/00923 cct Pro gtt Val gag Glu t tg Leu ttg Leu gag Glu acc Thr 150 act Thr gcc Ala atg Met ctg Leu gca Ala 230 gcg Ala gca Ala aac Asn gtg Val1 ttg Leu aag Lys gag Glu ct t Leu 9gc Gly 135 act Thr gtc Val acc Thr gc t Ala ga t Asp 215 tct Ser gtg Val1 gag Glu aac Asn gtg Val1 aac Asn gct Ala t cc S er 105 a tg Met t tg Leu acg Thr gga Gly ctg Leu 185 atc Ile gat Asp gct Ala gca Ala gtg Val1 265 cag Gln agc Ser gc t Ala cgt Arg ga t Asp ga t Asp gg t Gly gtg Val atg Met 170 gtc Val1 gcg Ala gga Gly agc Ser gc t Ala 250 acc Thr gcg Ala cga Arg ggt Gly gag Glu att Ile aag Lys gac Asp gat Asp 155 ggc Gly tgc Cys ctg Leu tca Ser gga Gly 235 tgt Cys aag Lys att Ile gtt Val1 aat Asn tct Ser tgt Cys 110 gca Ala gca Ala acc Thr g tg Val act Thr 190 gc t Ala acc Thr cca Pro atc Ile gct Ala 270 gc t Ala 9gC Gly aat Asn gc t Ala ggt Gly gat Asp gcc Ala 145 ttt Phe atg Met tcc S er gtt Val1 acc Thr 225 ga t Asp aag Lys gtg Val gt t Val1 cag Gin gg t Gly caa Gin ctt Leu cag Gln 130 aag Lys gct Ala gcg Ala gtt Val acg Thr 210 gtg Val1 gaa Glu ctt Leu gtg Val1 gc t Ala a tc Ile c tg Leu aat Asn att Ile 115 ctg Leu gcg Ala gat Asp ccg Pro act Thr 195 ttt Phe ttc Phe ctc Leu cag Gin gga Gly 275 cgt Arg 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 WO 01/00843 WO 0100843PCT/iBOO/00923 ttg ttc 1027 Leu Phe 295 ttg gct 1075 Leu Ala 310 tct gtg 1123 Ser Vai cct ggt 1171 Pro Gly att gat 1219 Ilie Asp gac ctg 1264 ttc ttc aat Phe Phe Asn 330 gct cgt gag Ala Arg Giu atg Met atg Met 315 ggt Gly gtg Val agt Ser tac ttt Phe 300 gc t Ala caa Gin gat Asp ggg Gly gtg gga Gly ga t Asp gca Ala ctt Leu gaa Giu 365 gag Glu tct gat cca aac Ser Asp Pro Asn 305 gct gat atg gaa Aia Asp Met Giu 320 gta tgc ctt gat Val Cys Leu Asp 335 tcc ggc gct gac Ser Gly Ala Asp 350 ggc cag gca aca Giy Gin Ala Thr atc aac tcc gcg Ile Asn Ser Ala 385 cgc Arg aag Lys ggc Gly 340 gtc Val acc Thr tct Ser gtg Val1 att Ile 325 gc t Al a cga Arg act Thr ttg Leu 360 agc 345 ggc Gly ttC Asp Leu Ser Phe Ser Tyr Val 375 380 taaaaagaaa cagcactcca act 1287 <210> 318 <211> 388 <212> PRT <213> Corynebacteriun giutamicum <400> 318 Met Ala Giu Lys Gly Ile Thr Ala Pro 1 5 Thr Thr Ala Gly Ilie Lys Ala Ser Gly Val Asn Gin Gly Pro Giu Phe Ser Ala Arg Val Phe Ala Ala Pro Val Lys Val Gly Gin Ile Arg Ala Val Leu Tyr Asn 70 Asn Gly Leu Gin Gly Giu Lys Asp Ala Ala Gin Asn Leu Gly Leu Giu Asp Ser 100 105 Ser Leu Arg Ala Ala His Ser Ala Val Asn Asp Cys Leu Thr WO 01/00843 PCT/IB00/00923 Gly Leu Ile Gly Glu Leu Leu Pro Met Asp Lys Leu Asn Ala Gly Ile 115 120 125 Asp Gin Leu Thr Ala Glu Gly Ala Leu Gly Asp Asn Gly Ala Ala Ala 130 135 140 Ala Lys Ala Ile Met Thr Thr Asp Thr Val Asp Lys Glu Thr Val Val 145 150 155 160 Phe Ala Asp Gly Trp Thr Val Gly Gly Met Gly Lys Gly Val Gly Met 165 170 175 Met Ala Pro Ser Leu Ala Thr Met Leu Val Cys Leu Thr Thr Asp Ala 180 185 190 Ser Val Thr Gin Glu Met Ala Gin Ile Ala Leu Ala Asn Ala Thr Ala 195 200 205 Val Thr Phe Asp Thr Leu Asp Ile Asp Gly Ser Thr Ser Thr Asn Asp 210 215 220 Thr Val Phe Leu Leu Ala Ser Gly Ala Ser Gly Ile Thr Pro Thr Gin 225 230 235 240 Asp Glu Leu Asn Asp Ala Val Tyr Ala Ala Cys Ser Asp Ile Ala Ala 245 250 255 Lys Leu Gin Ala Asp Ala Glu Gly Val Thr Lys Arg Val Ala Val Thr 260 265 270 Val Val Gly Thr Thr Asn Asn Glu Gin Ala Ile Asn Ala Ala Arg Thr 275 280 285 Val Ala Arg Asp Asn Leu Phe Lys Cys Ala Met Phe Gly Ser Asp Pro 290 295 300 Asn Trp Gly Arg Val Leu Ala Ala Val Gly Met Ala Asp Ala Asp Met 305 310 315 320 Glu Pro Glu Lys Ile Ser Val Phe Phe Asn Gly Gin Ala Val Cys Leu 325 330 335 Asp Ser Thr Gly Ala Pro Gly Ala Arg Glu Val Asp Leu Ser Gly Ala 340 345 350 Asp Ile Asp Val Arg Ile Asp Leu Gly Thr Ser Gly Glu Gly Gin Ala 355 360 365 Thr Val Arg Thr Thr Asp Leu Ser Phe Ser Tyr Val Glu Ile Asn Ser 370 375 380 Ala Tyr Ser Ser 385 <210> 319 <211> 1074 <212> DNA <213> Corynebacterium glutamicum WO 01/00843 WO 0100843PCTIBOOIOO923 <220> <221> CDS <222> (101)..(1051) <223> RXA02156 aaccactgac ctgagcttct cctacgtgga gatcaactcc aacagcactc caactaacaa gcagggaaaa gggcacaggc gcgtacagct cttaaaaaga atg aat gac ttg atc 11 Met Asn Asp Leu Ile 1 aaa gat tta ggc Lys Asp Leu Gly gag gtg cgc gca Giu Val Arg Ala aat Asn gtc ctc gct gag Val Leu Ala Giu gcg ttg Ala Leu cca tgg ttg Pro Trp Leu gga aac gcc Gly Asn Ala cag Gin cac ttc cgc gac His Phe Arg Asp att gtt gtc gtg Ile Val Val Val aaa tat ggc Lys Tyr Gly gct gcc gac Ala Ala Asp atg gtg gat gat Met Val Asp Asp ctc aag gct gct Leu Lys Ala Ala atg gtc Met Val ttc ttg cgc acc Phe Leu Arg Thr ggc gca aaa cca Gly Ala Lys Pro gtg gtg cac ggt Val Val His Gly ggt Gly gga cct cag att Gly Pro Gin Ilie gag atg cta aac cgt Giu Met Leu Asn Arg 80 gtg ggt ctc cag Val Gly Leu Gin ggc Gly gag ttc aag ggt Giu Phe Lys Gly ttc cgt gtg acc Phe Arg Val Thr act Thr cct gag gtc atg Pro Giu Val Met gac att Asp Ile 100 gtg cgc atg gtg ctc ttt ggt cag gtc ggt cgc gat tta Val Arg Met Val Leu Phe Gly Gin Val Gly Arg Asp Leu 105 110 gtt ggt ttg Val Gly Leu 115 gag gat gcc Giu Asp Ala atc aac tct Ile Asn Ser 120 cat ggc cct tac His Gly Pro Tyr gc t Ala 125 gtg gga acc tcc Val Gly Thr Ser ggc ctg Gly Leu 135 ttt acc gcg cag Phe Thr Ala Gin aag Lys 140 cgc atg gtc aac atc gat ggc gta ccc Arg Met Val Asn Ile Asp Gly Val Pro act Thr 150 gat att ggt ttg Asp Ilie Gly Leu gga gac atc att aat gtc gat gcc tct Gly Asp Ile Ilie Asn Val Asp Ala Ser 160 ttg atg gat atc Leu Met Asp Ilie gag gcc ggt cgc Giu Ala Gly Arg att Ile 175 cct gtg gtc tct Pro Val Val Ser acg att Thr Ile 180 gct cca ggc Ala Pro Gly gac ggc cag att Asp Gly Gin Ilie aac att aac gcc Asn Ile Asn Ala gat acc gca Asp Thr Ala 195 gca ggt gct ttg gct gca gcg att ggt gca gaa cgc ctg ctg gtt ctc WO 01/00843 WO 0100843PCTA~BOO/00923 Ala Gly Ala Leu Ala Ala Ala Ile Gly Ala Giu Arg Leu Leu Val Leu 200 205 210 acc aat gtg gaa ggt ctg tac acc Thr Asri Val Giu Gly Leu Tyr Thr 215 220 gtg tcc aag atc aag gcc acc gag Val Ser Lys Ilie Lys Ala Thr Glu 230 235 gat tcc ggc atg att cca aag atg Asp Ser Gly Met Ilie Pro Lys Met 250 ggg gga gta agc gct gct cat gtc Gly Gly Val Ser Ala Ala His Vai 265 gtg ttg ctg gag ctt ttg acc atg Val Leu Leu Giu Leu Leu Thr Met 280 285 ccg gat gtt ttt gat cgg gag aat 1027 Pro Asp Val Phe Asp Arg Glu Asn 295 300 aaa gac gac aag gat ggg gaa ctg 1074 Lys Asp Asp Lys Asp Giy Glu Leu 310 315 <210> 320 <211> 317 <212> PRT <213> Corynebacteriun giutamicur <400> 320 Met Asn Asp Leu Ilie Lys Asp Leu 1 5 Leu Ala Glu Ala Leu Pro Trp Leu Val Val Lys Tyr Giy Giy Asn Ala Ala Phe Ala Ala Asp Met Val Phe Val Val Vai His Gly Giy Giy Pro 70 Val Gly Leu Gin Gly Giu Phe Lys Giu Val Met Asp Ile Val Arg Met 100 gat Asp c tg Leu gag Giu att Ile 270 ggt Gly tgg Trp gag Glu tc t Ser 255 gac Asp gga.

Giy cct gat Pro Asp 225 gcc att Ala Ile 240 tgc ttg Cys Leu ggc Gly att Ile aag Lys ctt Leu aac Asn atc Ilie acg Thr 290 ac c agc tca ctg Ser Ser Leu ccg gga ctt Pro Gly Leu 245 gcg gtg cgt Ala Val Arg 260 gcg cac tcg Ala His Ser 275 atg gtg ctg Met Val Leu gtt ttt aga cgc Arg ggc Gly tat cct gaa ggc Tyr Pro Glu Giy Thr Val Phe Arg 305 taaatgagca cgctggaaac ttg Gly Gin Met Leu Gln Giy Val 105 Glu Phe Asp Thr Ser 75 Phe Phe Asn Ile Lys Lys Asn Thr Gly Val Val Ala Pro Arg Pro Arg WO 01/00843 Asp Leu Val 115 Ser Gly Giu 130 Ile Asp Gly 145 Val Asp Ala Val Val Ser Asn Ala Asp 195 Arg Leu Leu 210 Asp Lys Ser 225 Ile Leu Pro Leu Asn Ala Arg Ilie Ala 275 Gly Thr Met 290 Gly Thr Val 305 <210> 321 <211> 903 <212> DNA Leu Ala Pro Ser 165 Ile Ala Leu Leu Leu 245 Arg Ser Leu Arg His Thr Gly Ile Glu 185 Leu Glu Ile Met Ser 265 Giu Phe Lys Gly Ala Leu Ile 170 Asp Ala Gly Lys Ile 250 Ala Leu Asp Asp Pro Gin Val1 155 Giu Giy Ala Leu Ala 235 Pro Ala Leu Arg Giy 315 Ala 125 Arg Asp Gly Ile Ile 205 Thr Glu Met Val Met 285 Asn Leu Val1 Met Ile Arg Tyr 190 Gly Asp Leu Giu Ile 270 Gly Tyr PCTIIBOOIOO923 Gly Thr Val Asn Ile Asn 160 Ile Pro 175 Asn Ile Ala Giu Trp Pro Giu Ala 240 Ser Cys 255 Asp Giy Gly Ile Pro Giu <213> Corynebacteriun glutanicum <22 0> <221> CDS <222> (101)..(880) <223> RXN02153 <400> 321 cccacttgca gttttagctg taggtgggtt tttgcatgtc taacccgtct tttatgcaca cccccgcaat gaatcaaaaa tttatgcatg aataatttgc atg atc atg cat aac 115 Met Ilie Met His Asn 1 gtg tat ggt gta act atg aca atc aag gtt gca atc gca gga gcc agt 163 Vai Tyr Giy Val Thr Met Thr Ile Lys Val Ala Ile Ala Gly Ala Ser 15 WO 01/00843 gga tat gcc Gly Tyr Ala tat gca tct Tyr Ala Ser PCTIiBOO/00923 gga gaa atc ctt Gly Glu Ile Leu cgt Arg ctc ctt tta ggc Leu Leu Leu Gly cat cca gct His Pro Ala gca tca acc Ala Ser Thr ggt gaa cta gaa Gly Giu Leu Giu gga gca ctc acc Gly Ala Leu Thr gca ggc Ala Gly agc acg ctc ggt Ser Thr Leu Gly ttg atg cca cac Leu Met Pro His ccg cag ttg gcg Pro Gin Leu Ala gat Asp cgt gtt att caa Arg Val Ilie Gin acc aca gct gaa Thr Thr Ala Glu cta gcc ggt cat Leu Ala Gly His gat Asp gtc gta ttt cta Val Val Phe Leu ctt cca cac gga Leu Pro His Gly tct gca gaa att Ser Ala Giu Ile gca ctt Ala Leu 100 cag ctc gga Gin Leu Gly gat gtc aca gtg Asp Val Thr Val att Ile 110 gac tgt gca gct Asp Cys Ala Ala gac ttt cgt Asp Phe Arg 115 gag cac cag Glu His Gin ctg caa aat gct gca gat tgg Leu Gin Asn Ala Ala Asp Trp 120 gga aca tgg cct tat ggc att Gly Thr Trp Pro Tyr Gly Ile gag Giu 125 aag ttc tac ggc Lys Phe Tyr Gly cca gaa atg cca Pro Glu Met Pro cac cgc gag gct His Arg Giu Ala ctt Leu 150 cgt ggt gct aag Arg Gly Ala Lys gta gca gtg cca Val Ala Val Pro tgt ttc cca acc Cys Phe Pro Thr ggt Gly 165 gca acc ttg gct Ala Thr Leu Ala ctt cct gcg gtt Leu Pro Ala Val gcg gga ctt atc Ala Gly Leu Ile gag cca Giu Pro 180 gat gtt tcc Asp Val Ser gca tct gtt Ala Ser Val 200 gtg tcc atc acc Val Ser Ile Thr ggc Gly 190 gta tca ggt gca Val Ser Gly Ala ggt aag aaa Gly Lys Lys 195 ctc aag gcg Leu Lys Ala gca cta ctt ggc Ala Leu Leu Gly tcg Ser 205 gaa acc atg ggt Giu Thr Met Gly tac aac acc tcc gga aag Tyr Asn Thr Ser Gly Lys cgc cac acc ccg Arg His Thr Pro att gcc cag aac Ile Ala Gin Asn ctc Leu 230 ggc gaa gtc agc Giy Giu Val Ser aag cca gtc aag Lys Pro Val Lys agc ttc acc cca Ser Phe Thr Pro gtg Val 245 ctt gca ccg tta Leu Ala Pro Leu cgc gaa ttc tca Arg Glu Phe Ser ctg caa ccg cac Leu Gin Pro His tgaaagaagg cgttaccgca gaa WO 01/00843 WO 0100843PCT/EBOO/00923 <210> 322 <211> 260 <212> PRT <213> Corynebacterium giutaiicui <400> 322 Met Ile Met His Asn Val Tyr Gly Val 1 Ile Leu Thr Ile Leu Al a Ala Gly Gly 145 Cys Gly Gly Gly Giu 225 Ser Gin Gly Tyr Ala Asp 70 Val Gin Leu Gly Leu 150 Ala Asp Ala Tyr Leu 230 Leu Tyr Ala Gly Arg Vali Leu Gin Thr 135 Arg Thr Val Ser Asn 215 Gly Ala Thr 10 Gly Giu Leu Gin Gly Asp Ala Tyr Lys Leu 170 Val Leu Gly Ser Pro 250 Met Giu Leu Gly Asp 75 Leu Val1 Asp Gly Arg 155 Leu Ser Leu Lys

ASP

235 Arg Thr Ile Giu Giu Thr Pro Thr Trp, Ile 140 Val Pro Ile Gly His 220 Lys Glu Val1 Leu Ala Pro Giu Phe Asp Phe Met Pro Gin 175 Val1 Thr Thr Lys Pro 255 Ala Leu Leu His Thr Ser Cys Tyr Pro Gly 160 Ala Ser Met Pro Val1 240 Leu <210> 323 WO 01/00843 WO 0100843PCT/iBOO/00923 <211> <212> <213> <220> <221> <222> <223> 903

DNA

Corynebac terium glutamicum

CDS

(101)..(880) FRXA0 2153 <400> 323 cccacttgca gttttagctg taggtgggtt tttgcatgtc taacccgtct tttatgcaca dccccgcaat gaatcaaaaa tttatgcatg aataatttgc atg atc atg cat Met Ile Met His gtg tat ggt gta Val Tyr Gly Val atg aca atc aag gtt gca atc gca gga Met Thr Ile Lys Val Ala Ilie Ala Gly gcc agt Ala Ser gga tat gcc Gly Tyr Ala tat gca tct Tyr Ala Ser gga gaa atc ctt Gly Glu Ile Leu ctc ctt tta ggc Leu Leu Leu Gly cat cca gct His Pro Ala gca tca acc Ala Ser Thr ggt gaa cta gaa Gly Giu Leu Giu gga gca ctc acc Gly Ala Leu Thr gca ggc Ala Gly agc acg ctc ggt Ser Thr Leu Gly ttg atg cca cac Leu Met Pro His ccg cag ttg gcg Pro Gin Leu Ala gat cgt gtt att caa gac acc aca gct gaa act Asp Arg Val Ile Gin ASP Thr Thr Ala Glu Thr 75 cta gcc ggt cat Leu Ala Gly His gtc gta ttt cta Val Val Phe Leu ctt cca cac gga Leu Pro His Giy tct gca gaa att Ser Ala Giu Ile gca ctt Aia Leu 100 cag ctc gga Gin Leu Gly ctg caa aat Leu Gin Asn 120 gat gtc aca gtg Asp Val Thr Vai gac tgt gca gct Asp Cys Ala Aia gac ttt cgt Asp Phe Arg 115 gag cac cag Glu His Gin gct gca gat tgg Ala Ala Asp Trp gag Giu 125 aag ttc tac ggc Lys Phe Tyr Gly tca Ser 130 gga aca Giy Thr 135 tgg cct tat ggc Trp Pro Tyr Giy att Ile 140 cca gaa atg cca Pro Giu met Pro gga Gly 145 cac cgc gag gct His Arg Giu Ala ctt Leu 150 cgt ggt gct aag Arg Gly Ala Lys gta gca gtg cca Val Ala Val Pro gga tgt ttc cca acc ggt Gly Cys Phe Pro Thr Gly 160 165 gca acc ttg gct Ala Thr Leu Ala ctt cct gcg gtt Leu Pro Ala Val caa Gin 175 gcg gga ctt atc Ala Gly Leu Ile gag cca Giu Pro 180 gat gtt tcc gta gtg tcc atc acc ggc gta tca ggt gca ggt aag aaa WO 01/00843 Asp Val Ser Val Val Ser Ile Thr Gly 185 190 gca tct gtt gca cta ctt ggc tcg gaa Ala Ser Val Ala Leu Leu Gly Ser Giu 200 205 tac aac acc tcc gga aag cac cgc cac Tyr Asn Thr Ser Gly Lys His Arg His 215 220 ctc ggc gaa gtc agc gac aag cca gtc Leu Gly Giu Val Ser Asp Lys Pro Val 230 235 ctt gca ccg tta cct cgc gaa ttc tca Leu Ala Pro Leu Pro Arg Giu Phe Ser 250 tgaaagaagg cgttaccgca gaa <210> 324 <211> 260 <212> PRT <213> Corynebacterium giutainicum <400> 324 Met Ile Met His Asn Val Tyr Gly Val Ser atg Met ccg Pro gtg Val 240 ctg Leu PCTIBOO/00923 (s Lys 19 gcg 739 Y's Ala caa ccg cac Gin Pro His 1 Ile Leu Thr Ile Leu Al a Ala Gly Gly 145 Cys Ala Pro Ser Leu His Ala 100 Phe His Glu Thr 5 Ser Ala Thr Ala Asp Leu Arg Gin Ala Gly 165 Thr Ile Giu Giu Thr Pro Thr Trp Ile 140 Val1 Pro Ile Leu Ile Leu Thr His Val1 Giu 125 Pro Ala Ala Lys Arg Gly Met Ala Gly Ile 110

LYS

Giu Val1 Val1 Val1 Leu Ala Pro Giu Phe Asp Phe Met Pro Gin 175 Ala Leu Leu His Thr Ser Cys Tyr Pro Gly 160 Ala WO 01/00843 Gly Leu Il Gly Ala G1 19 Gly Ser Le 210 Glu Ile Al 225 Ser Phe Tb Gin Pro Hi Asp Val Ser Val 185 Ala Ser Val Ala 200 Tyr Asn Thr Ser 215 Leu Gly Glu Val 230 Leu Ala Pro Leu Val Leu Gly Ser Pro 250 Ser Ile Thr Gly 190 Leu Gly Ser Glu 205 Lys His Arg His 220 Asp Lys Pro Val 235 Arg Glu Phe Ser PCTIBOO/00923 Val Ser Thr met Thr Pro Lys Val 240 Pro Leu 255 <210> 325 <211> 414 <212> DNA <213> Corynebacteriun glutanicum' <220> <221> CDS <222> (101)..(391) <223> RXA02154 <400> 325 cccagaacct cggcgaagtc agcgacaagc cagtcaaggt caccgttacc tcgcgaattc tcaccactgc aaccgcacct acc gca gaa cag Thr Ala Glu Gin acc ttc gtg cat Thr Phe Val His gtt ctt ggc tcc Val Leu Gly Ser gca ggc aaa gtc Ala Gly Lys Val act gcc ggc gcc Thr Ala Gly Ala gag gca gca ggc Giu Ala Ala Gly ccttaaagcg gcg gca Ala cca Pro tgC Cys acc Thr 60 cag Gin cag Gin tat Tyr ggt Gly 30 gtg Val1 gca Ala atg Met ggc Gly gaa Glu gca Ala cag Gin atc Ile aac Asn gtc Va 1 gagcttcacc ccagtgcttg ttg aaa gaa ggc gtt 115 Leu Lys Glu Gly Val 1 ttc tat gca cag gaa 163 Phe Tyr Ala Gin Giu cca caa acc caa gca 211 Pro Gin Thr Gin Ala gaa att gat gag gaa 259 Giu Ile Asp Giu Glu aac ctc acc aag gga 307 Asn Leu Thr Lys Gly agc gtt ggt ttt gat 355 Ser Val Gly Phe Asp cct taaagtagcg 401 Pro WO 01/00843 WO 0100843PCT/IBOO/00923 <210> 326 <211> 97 <212> PRT <213> Corynebacteriun giutamicun <400> 326 Leu Lys Giu Gly Val Thr Ala Giu 1 5 Phe Tyr Ala Gin Giu Thr Phe Val 1 Pro Gin Thr Gin Ala Val Leu Gly Giu Ilie Asp Giu Giu Ala Gly Lys 1 Asn Leu Thr Lys Gly Thr Ala Gly 1 Ser Val Gly Phe Asp Giu Ala Ala Pro Tyr Gly Val Ala Met Giy Giu Al a Gin Ile Asn Val <210> 327 <211> 1296 <212> DNA <213> Corynebacteriun giutanicun <22 0> <221> CDS <222> (101)..(1273) <223> RXA02157 <400> 327 gggtggaatt ggcacgatgg tgctgccgga tgtttttgat caccgttttt agaaaagacg acaaggatgg ggaactgtaa act Thr ctg Leu tac Tyr cac His gtc att att Val Ile Ile aag ggc gca Lys Gly Ala ctc gcg ggc Leu Ala Gly atc gag gcg le Glu Ala aa t Asn acc Thr atc Ile gtc Val tac ggc Tyr Gly 15 act gat Thr Asp gtc aac Val Asn aac cag Asn Gin cgggagaatt atcctgaagg atg agc acg ctg gaa Met Ser Thr Leu Giu 1 acc cca cca gtt gag Thr Pro Pro Val Giu gac cag ggc aat gtc Asp Gin Gly Asn Val gcg ttg ggc cac gcc Ala Leu Gly His Ala atc ggc caa ctt ggt Ile Gly Gin Leu Gly 115 163 211 259 307 WO 01/00843 PCT/iBOO/00923 60 cac gtc tca aac ttg ttc gca tcc agg ccc gtc gtc gag gtc gcc gag 355 His Val Ser Asn Leu Phe Ala Ser Arg Pro Val Val Giu Val Ala Giu 75 80 gag ctc atc aag cgt ttt tcg ctt gac gac gcc acc ctc gcc gcg caa 403 Giu Leu Ile Lys Arg Phe Ser Leu Asp Asp Ala Thr Leu Ala Ala Gin 95 100 acc cgg gtt ttc ttc tgc aac tcg ggc gcc gaa gca aac gag gct gct 451 Thr Arg Val Phe Phe Cys Asn Ser Gly Ala Giu Ala Asn Giu Ala Ala 105 110 115 ttc aag att gca cgc ttg act ggt cgt tcc cgg att ctg gct gca gtt 499 Phe Lys Ile Ala Arg Leu Thr Gly Arg Ser Arg Ile Leu Ala Ala Val 120 125 130 cat ggt ttc cac ggc cgc acc atg ggt tcc ctc gcg ctg act ggc cag 547 His Gly Phe His Gly Arg Thr Met Gly Ser Leu Aia Leu Thr Giy Gin 135 140 145 cca gac aag cgt gaa gcg ttc ctg cca atg cca agc ggt gtg gag ttc 595 Pro Asp Lys Arg Giu Ala Phe Leu Pro Met Pro Ser Gly Val Glu Phe 150 155 160 165 tac cct tac ggc gac acc gat tac ttg cgc aaa atg gta gaa acc aac 643 Tyr Pro Tyr Gly Asp Thr Asp Tyr Leu Arg Lys Met Val Giu Thr Asn 170 175 180 cca acg gat gtg gct gct atc ttc ctc gag cca atc cag ggt gaa acg 691 Pro Thr Asp Val Ala Ala Ile Phe Leu Giu Pro Ile Gin Gly Giu Thr 185 190 195 ggc gtt gtt cca gca cct gaa gga ttc ctc aag gca gtg cgc gag ctg 739 Gly Val Val Pro Ala Pro Giu Gly Phe Leu Lys Ala Val Arg Glu Leu 200 205 210 tgc gat gag tac ggc atc ttg atg atc acc gat gaa gtc cag act ggc 787 Cys Asp Giu Tyr Gly Ilie Leu Met Ilie Thr Asp Glu Val Gin Thr Gly 215 220 225 gtt ggc cgt acc ggc gat ttc ttt gca cat cag cac gat ggc gtt gtt 835 Val Gly Arg Thr Gly Asp Phe Phe Ala His Gin His Asp Gly Val Val 230 235 240 245 ccc gat gtg gtg acc atg gcc aag gga ctt ggc ggc ggt ctt ccc atc 883 Pro Asp Val Val Thr Met Ala Lys Gly Leu Gly Gly Gly Leu Pro Ile 250 255 260 ggt gct tgt ttg gcc act ggc cgt gca gct gaa ttg atg acc cca ggc 931 Gly Ala Cys Leu Ala Thr Gly Arg Ala Ala Giu Leu Met Thr Pro Gly 265 270 275 aag cac ggc acc act ttc ggt ggc aac cca gtt gct tgt gca gct gcc 979 Lys His Gly Thr Thr Phe Gly Gly Asn Pro Val Ala Cys Ala Ala Ala 280 285 290 aag gca gtg ctg tct gtt gtc gat gac gct ttc tgc gca gaa gtt gcc 1027 Lys Ala Val Leu Ser Val Val Asp Asp Ala Phe Cys Ala Giu Val Ala WO 01/00843 295 300 cgc aag ggc gag ctg ttc aag gaa c 1075 Arg Lys Gly Giu Leu Phe Lys Giu 1 310 315 gta gac gtc cgt ggc agg ggc ttg e 1123 Val Asp Val Arg Gly Arg Gly Leu b 330 gac gtc gca aag caa gct gtt ctt 1171 Asp Val Ala Lys Gin Ala Val Leu 1 345 ttg aat gca ccg gcg gac aac att 1219 Leu Asn Ala Pro Ala Asp Asn Ilie 360 365 atc acc gac gaa gaa atc gca gac 1267 Ilie Thr Asp Glu Giu Ilie Ala Asp2 375 380 atc gca taaaggactc aaacttatga ctt 1296 Ilie Ala 390 <210> 328 <211> 391 <212> PRT <213> Corynebacterium glutarnicum <400> 328 Met Ser Thr Leu Glu Thr Trp Pro 1 5 Thr Pro Pro Val Glu Leu Val Ser Asp Gin Giy Asn Val Tyr Ile Asp 40 Ala Leu Gly His Ala His Pro Ala Ilie Giy Gin Leu Gly His Val Ser2 Val Giu Val Ala Glu Glu Leu Ile Thr Leu Ala Ala Gin Thr Arg Val 100 Ala Asn Giu Ala Ala Phe Lys Ilie2 PCT/1B00100923

.D

ctt Leu ttg Leu 335 gg t Gly cgt Arg gtc Val1 Val Lys Leu Ile Leu Arg Phe Arg gtt Val1 gtg Val cac His ccg Pro 370 att Ile Asn Thr Ile Val Ser Leu Ser Gly gac Asp ctg Leu ggc Gly 355 ccg Pro gcc Ala Thr Val Ala Thr Arg Asp C ly 110 Arg 31n Aly 25 eu Ile ksn ys The 487 WO 01/00843 WO 0100843PCT/IBOO/00923 115 120 125 Ile Leu Ala Ala Val His Gly Phe His Gly Arg Thr Met Gly Ser Leu 130 135 140 Ala Leu Thr Gly Gin Pro Asp Lys Arg Giu Ala Phe Leu Pro Met Pro 145 150 155 160 Ser Gly Val Giu Phe Tyr Pro Tyr Gly Asp Thr Asp Tyr Leu Arg Lys 165 170 175 Met Val Giu Thr Asn Pro Thr Asp Val Ala Ala Ile Phe Leu Glu Pro 180 185 190 Ile Gin Gly Giu Thr Gly Val Val Pro Ala Pro Giu Gly Phe Leu Lys 195 200 205 Ala Val Arg Giu Leu Cys Asp Glu Tyr Gly Ile Leu Met Ile Thr Asp 210 215 220 Giu Val Gin Thr Gly Val Gly Arg Thr Gly Asp Phe Phe Ala His Gin 225 230 235 240 His Asp Gly Val Val Pro Asp Val Val Thr Met Ala Lys Gly Leu Gly 245 250 255 Gly Gly Leu Pro Ilie Gly Ala Cys Leu Ala Thr Gly Arg Ala Ala Giu 260 265 270 Leu Met Thr Pro Gly Lys His Gly Thr Thr Phe Gly Gly Asn Pro Val 275 280 285 Ala Cys Ala Ala Ala Lys Ala Val Leu Ser Val Val Asp Asp Ala Phe 290 295 300 Cys Ala*Giu Val Ala Arg Lys Gly Giu Leu Phe Lys Giu Leu Leu Ala 305 310 315 320 Lys Val Asp Gly Val Val Asp Val Arg Gly Arg Gly Leu Met Leu Gly 325 330 335 Val Val Leu Giu Arg Asp Val Ala Lys Gin Ala Val Leu Asp Gly Phe 340 345 350 Lys His Gly Val Ile Leu Asn Ala Pro Ala Asp Asn Ile Ile Arg Leu 355 360 365 Thr Pro Pro Leu Val Ile Thr Asp Giu Giu Ile Ala Asp Ala Val Lys 370 375 380 Ala Ile Ala Giu Thr Ile Ala 385 390 <210> 329 <211> 1491 <212> DNA <213> Corynebacterium giutainicum <22 0> <221> CDS WO 01/00843 <222> (101)..(1468) <223> RXS02970 <400> 329 aaccgacaaa acagccgttc acgtgctaaa gcagctcggc PCTIBO0100923 ttgatctagg gtgaggtgag ttatttaaag acttcataat attttgggga gtgaactggt ttg gca Leu Ala ttg aag ggt Leu Lys Gly tac Tyr aaa Lys cac His gcc Ala atg Met tta Leu ccg Pro tcg Ser gcc Ala cgc Arg 150 tcc Ser acc Thr acc Thr gaa Giu t cc Ser gaa Giu ggt Gly gtt Val1 gcc Ala atg Met gac

ASP

135 aac Asn gcg Ala gac Asp aac Asn gag Giu tgg Trp gg t Gly tc c Ser gag Giu t tc Phe gcc Ala 120 gcc Ala aaa Lys atg Met cca Pro ttt Phe gaa Glu tcc Ser tcc Ser c aa Gin gcg Ala ggC Gly 105 cgt Arg atc Ile att Ilie atg Met gat Asp 185 gac Asp aaa Lys gcg Ala acg Thr ctt Leu atc Ilie aat Asn ggc Gly gag Giu ctg Leu c tc Leu 170 atc Ile ggt Gly cgg Arg cag Gin c tg Leu gtc Val1 75 cag Gin gat Asp gaa Giu cac His tcc Ser 155 acc Thr tac Tyr gaa Glu gca Ala gac Asp tac Tyr 60 tcg Ser cgc Arg gtg Val t tc Phe tcc Ser 140 gca Ala ggc Gly cac His t tc Phe t tc Phe aaa Lys 45 gac Asp gca Ala caa Gin cgc Arg tcc Ser 125 atc Ilie tac Tyr gaa Giu t tc Phe atc Ile gac Asp 30 atc Ile t tc Phe aac Asn gca Aia tct Ser 110 cac His cgc Arg cgc Arg cac His tg Trp 190 gaa gaa Glu 15 aac Asn agc Ser gac Asp tta Leu gcc Ala 95 gat Asp gtg Val atg Met agc Ser cgc Arg 175 gca Ala gaa Glu t tc Phe gat Asp ccc Pro ggc Gly ggc Gly 80 cgg Arg gt t Val1 ttt Phe gc t Ala tac Tyr 160 cgc Arg c ca Pro tgc Cys 1 gga Gly cgc Arg aaa Lys aa c Asn 65 cac His ttg Leu gct Ala ttc Phe cgc Arg 145 cac His ctg Leu ttc Phe gaa Glu caa Gin 20 gtt Val gca Ala atc Ile cc t Pro atc Ile 100 atc Ile Gly acc Thr acc Thr ccc Pro 180 cac His c tc Leu gca Ala t tc Phe gc t Ala gac Asp cga Arg aac Asn gtg Val ggc Gly gga Gly gga Gly 165 acc Thr tcc Ser aag Lys 115 163 211 259 307 355 403 451 499 547 595 643 691 739 tca ttc ttt gcc acc acc caa gaa Ser Phe Phe Ala Thr Thr Gin Giu Giu WO 01/00843 WO 0100843PCTIiBOOIO0923 cac ttg His Leu 215 atc gtc Ile Val 230 gca ggt Ala Gly ctc ttc Leu Phe ctg ttt Leu Phe acc ttc 1027 Thr Phe 295 gtg atg 1075 Val Met 310 ggc gga 1123 Gly Gly gca gcg 1171 Ala Ala cga ctt 1219 Arg Leu gaa aac 1267 Glu Asn 375 gtg gag 1315 Val Glu 390 ttc aag 1363 Phe Lys gaa Glu ctg Leu tac Tyr atc Ile gc t Ala 280 gcc Ala acc Thr ctc Leu ctg Leu ggc Gly 360 gta Val ttc Phe gaa Glu gat Asp gag Glu tta Leu gcc Ala 265 tac Tyr aag Lys c aa Gin acc Thr gag Glu 345 gc t Ala gcg Ala aat Asn cgc Arg gtc Val1 cca Pro aa t Asn 250 gac Asp gag Glu ggt Gly tca Ser tac Tyr 330 att Ile gaa Glu atc Ile gca Ala ggc Gly 410 a tc Ile gtg Val 235 ggc Gly gaa Glu cat His gt t Val atc Ile 315 tcc Ser tac Tyr ctg Leu gc t Ala gac Asp 395 gtg Val1 gcg Ala 220 gtg Val gtg Val gtc Val gct Ala aac Asn 300 cgc Arg gga Gly gcg Ala atc Ile gac Asp 380 gcc Ala tgg Trp ttt Phe gga Gly cgc Arg atg Met ggc Gly 285 gca Ala ga t Asp cac His gaa Glu gaa Glu 365 gtg Val1 act Thr ccg Pro a tc Ile ctg Leu cac His acc Thr 275 gac Asp ggt Gly gca Ala ccc Pro cgc Arg 355 cta Leu t tc Phe gct Ala cga Arg gca gcg Ala Ala cca cca Pro Pro 245 ggc atc Gly Ile 260 gga aaa Gly Lys atg atc Met Ile ggc atc Gly Ile tac tcc Tyr Ser 325 gcc aag Ala Lys 340 gta gct Val Ala gcg gaa Ala'Glu tgg gca Trp Ala gca gaa Ala Glu 405 ttc cac Phe His 420 787 835 883 931 979 WO 01/00843 atc gcg cc 1411 Ile Ala Pr gac gcg gt 1459 Asp Ala Va 44 gcg ttg tt 1491 Ala Leu Ph 455 PCTIBOO/00923 ccg ctg acc acc act gat gac gaa ttg gta gca ctg ctg Pro Leu Thr Thr Thr Asp Asp Giu Leu Val Ala Leu Leu 425 430 435 gaa gct gca gcc caa gdt gtc gag ctg acc ttc gct ggg Glu Ala Ala Ala Gin Ala Val Giu Leu Thr Phe Ala Gly 445 450 taagttttct agataacaag gcc <210> 330 <211> 456 <212> PRT <213> Corynebacterium giutanicui <400> 330 Leu Ala Leu Lys Tyr Thr Asn Phe

LYS

His Ala Met Leu Pro Ser Ala Arg 150 Ser Thr Ser Giu Ser Glu Gly Val Ala Met Asp 135 Asn Ala Asp Phe Asp Lys Ala Thr Leu Ile Asn Gly Glu Leu Leu 170 Ile Phe Phe.

Lys Asp Ala Gln Arg Ser 125 Ile Tyr Glu Phe Ile Asp Ile Phe Asn Ala Ser 110 His Arg Arg His Trp 190 Glu Asn Ser Asp Leu Ala Asp Val Met Ser Arg 175 Ala Thr Thr Gin Giu Glu Glu Cys 205 WO 01/00843 PCTIBOOIOO923 Giu Arg Ala Leu Lys His Leu Giu Asp Val Ile Ala Phe Giu Gly Ala 210 215 220 Gly Met Ile Ala Ala Ilie Val Leu Giu Pro Val Val Gly Ser Ser Gly 225 230 235 240 Ile Ilie Leu Pro Pro Ala Gly Tyr Leu Asn Giy Val Arg Giu Leu Cys 245 250 255 Asn Lys His Gly Ilie Leu Phe Ilie Ala Asp Giu Val Met Val Gly Phe 260 265 270 Gly Arg Thr Gly Lys Leu Phe Ala Tyr Giu His Ala Gly Asp Asp Phe 275 280 285 Gin Pro Asp Met Ile Thr Phe Ala Lys Gly Val Asn Ala Gly Tyr Aia 290 295 300 Pro Leu Gly Gly Ile Val Met Thr Gin Ser Ile Arg Asp Thr Phe Gly 305 310 315 320 Ser Giu Ala Tyr Ser Gly Gly Leu Thr Tyr Ser Gly His Pro Leu Ala 325 330 335 Val Ala Pro Ala Lys Ala Ala Leu Giu Ile Tyr Ala Giu Gly Giu Ile 340 345 350 Ilie Pro Arg Val Ala Arg Leu Gly Ala Giu Leu Ile Giu Pro Arg Leu 355 360 365 Arg Giu Leu Ala Glu Glu Asn Val Ala Ile Ala Asp Val Arg Gly Ile 370 375 380 Gly Phe Phe Trp Ala Val Giu Phe Asn Ala Asp Ala Thr Ala Met Ala 385 390 395 400 Ala Gly Ala Ala Giu Phe Lys Giu Arg Gly Val Trp Pro Met Ile Ser 405 410 415 Gly Asn Arg Phe His Ile Ala Pro Pro Leu Thr Thr Thr Asp Asp Glu 420 425 430 Leu Val Ala Leu Leu Asp Ala Val Giu Ala Ala Ala Gin Ala Val Giu 435 440 445 Leu Thr Phe Ala Gly Ala Leu Phe 450 455 <210> 331 <211> 1330 <212> DNA <213> Corynebacteriun giutamicum <22 0> <221> CDS <222> (1330) <223> FRXA01009 <400> 331 aaccgacaaa acagccgttc acgtgctaaa gcagctcggc ttgatctagg gtgaggtgag 492 WO 01/00843 WO 0100843PCTJLBOIO923 ttatttaaag acttcataat attttgggga gtgaactggt ttg gca ttg Leu Ala Leu t ac Tyr aaa Lys cac His gcc Ala atg Met tta Leu ccg Pro tcg Ser gcc Ala cgc Arg 150 tcc Ser acc Thr tca Ser cac His aac Asn gag Glu tgg Trp ggt Gly tcc Ser gag Glu ttc Phe gcc Ala 120 gcc Ala aaa Lys atg Met c ca Pro ttt Phe 200 gaa Glu ttt Phe gaa Glu t cc Ser t cc Ser caa Gln gcg Ala ggc Gly 105 cgt Arg atc Ile at t Ile atg Met ga t Asp 185 gcc Ala gat Asp ggt Gly cgg Arg cag Gln ctg Leu gtc Val 75 cag Gln ga t Asp gaa Glu cac His tcc Ser 155 ac c Thr tac Tyr acc Thr atc Ile gaa Glu aac Asn agc Ser gac Asp tta Leu gcc Ala gat Asp gtg Val atg Met agc Ser cgc Arg 175 gca Ala gaa Glu ggt Gly ttc Phe gat Asp ccc Pro ggc Gly ggc Gly cgg Arg gt t Val ttt Phe gc t Ala tac Tyr 160 cgc Arg cca Pro tgc Cys gc t Al a

I

gga Gly cgc Arg aaa Lys aac Asn cac His t tg Leu gc t Ala ttc Phe cgc Arg 145 c ac His ctg Leu t tc Phe gaa Glu ggc Gly 225 gtg Val cac His tgg Trp, ttc Phe aac Asn aac Asn aag Lys 115 aac Asn cac His gca Ala aac Asn cac His 195 gca Ala atc Ile aag Lys caa Gln gtt Val gca Ala atc I le cct Pro a tc Ile 100 atc Ile ggc Gly acc Thr acc Thr ccc Pro 180 cac His ctc Leu gca Ala WO 01/00843 WO 0100843PCTJLBOOIOO923 atc gtc ctg gag cca gtg gtg gga tca tca Ile Val Leu Glu Pro Val Val Gly Ser Ser gga atc atc ctg cca cca Gly Ile Ile Leu Pro Pro ggt tac tta Gly Tyr Leu gtg cgc gaa Val Arg Glu cttI Leu 5 gga Giy aac aag cac Asn Lys His ggc atc Gly Ile 260 835 883 931 979 ctc ttc Leu Phe ctg ttt Leu Phe acc ttc 1027 Thr Phe 295 gtg atg 1075 Val Met 310 ggc gga 1123 Gly Gly gca gcg 1171 Ala Ala cga ctt 1219 Arg Leu gaa aac 1 267 Glu Asn 375 gtg gag 1315 Val Glu 390 ttc aag 1330 Phe Lys atc Ile gct Al a 280 gcc Ala 265 tac Tyr gaa gtc atg Glu Val Met gtc Val1 270 gac Asp ttc gga cgc Phe Gly Arg acc gga aaa Thr Gly Lys 275 gag cat gct Glu His Ala ggc Gly 285 gat ttc cag Asp Phe Gin gcc aag ggt gtt aac Ala Lys Giy Val Asn 300 acc caa tca atc cgc Thr Gin Ser Ile Arg 315 ctc acc tac tcc gga Leu Thr Tyr Ser Gly gca ggt tac gcc cca Ala Gly Tyr Ala Pro 305 gat acc ttc gga tca Asp Thr Phe Gly Ser cca gac atg atc Pro Asp Met Ile 290 ctc ggt ggc atc Leu Gly Gly Ile gag gca tac tcc Giu Ala Tyr Ser 325 ctg gag Leu Glu 345 ggC gct Gly Ala 330 att Ile tac: gcg Tyr Ala cac cca ctt His Pro Leu 335 gaa gga gag Giu Gly Giu 350 gta gca ccc gcc aag Val Ala Pro Ala Lys 340 atc att cca cgc gta gct Ile Ile Pro Arg Val Ala 355 gaa ctg atc Glu Leu Ile gaa cct cgc ctt cgt gas cta. gcg gaa Giu Pro Arg Leu Arg Glu Leu Ala Glu 365 370 gtg cgg ggc atc gga ttc ttC tgg gca Val Arg Gly Ile Gly Phe Phe Trp Ala gcg atc gct gac Ala Ile Ala Asp 380 ttc aat gca gac Phe Asn Ala Asp 395 gcc act gcc atg gct Ala Thr Ala Met Ala 400 ggt gct gca gaa Gly Ala Ala Giu 405 gaa cgc ggc Glu Arg Gly 410 <210> 33? <211> 410 <212> PRT <213> Corynebacteriun glutaricum WO 01/00843 PCT/IB00/00923 <400> 332 Leu Ala Leu Lys Gly Tyr Thr Asn Phe Asp Gly Glu Phe Ile Glu Phe 1 5 10 Gly Ser Val Gin Ala Lys Glu Glu Glu Lys Arg Ala Phe Asp Asn Asp 25 Arg Ala His Val Phe His Ser Trp Ser Ala Gin Asp Lys Ile Ser Pro 40 Lys Val Trp Ala Ala Ala Glu Gly Ser Thr Leu Tyr Asp Phe Asp Gly 55 Asn Ala Phe Ile Asp Met Gly Ser Gin Leu Val Ser Ala Asn Leu Gly 70 75 His Asn Asn Pro Arg Leu Val Glu Ala Ile Gin Arg Gin Ala Ala Arg 90 Leu Thr Asn Ile Asn Pro Ala Phe Gly Asn Asp Val Arg Ser Asp Val 100 105 110 Ala Ala Lys Ile Val Ser Met Ala Arg Gly Glu Phe Ser His Val Phe 115 120 125 Phe Thr Asn Gly Gly Ala Asp Ala Ile Glu His Ser Ile Arg Met Ala 130 135 140 Arg Leu His Thr Gly Arg Asn Lys Ile Leu Ser Ala Tyr Arg Ser Tyr' 145 150 155 160 His Gly Ala Thr Gly Ser Ala Met Met Leu Thr Gly Glu His Arg Arg 165 170 175 Leu Gly Asn Pro Thr Thr Asp Pro Asp Ile Tyr His Phe Trp Ala Pro 180 185 190 Phe Leu His His Ser Ser Phe Phe Ala Thr Thr Gin Glu Glu Glu Cys 195 200 205 Glu Arg Ala Leu Lys His Leu Glu Asp Val Ile Ala Phe Glu Gly Ala 210 215 220 Gly Met Ile Ala Ala Ile Val Leu Glu Pro Val Val Gly Ser Ser Gly 225 230 235 240 Ile Ile Leu Pro Pro Ala Gly Tyr Leu Asn Gly Val Arg Glu Leu Cys 245 250 255 Asn Lys His Gly Ile Leu Phe Ile Ala Asp Glu Val Met Val Gly Phe 260 265 270 Gly Arg Thr Gly Lys Leu Phe Ala Tyr Glu His Ala Gly Asp Asp Phe 275 280 285 Gin Pro Asp Met Ile Thr Phe Ala Lys Gly Val Asn Ala Gly Tyr Ala 290 295 300 Pro Leu Gly Gly Ile Val Met Thr Gin Ser Ile Arg Asp Thr Phe Gly 305 310 315 320 WO 01/00843 WO 0100843PCTIBOOIOO923 Ser Val Ile Arg Gly 385 Ala Gly Ala Leu Asn 375 Giu Lys Gly His Pro Leu 335 Ala Glu Gly Glu 350 Ile Glu Pro Arg 365 Asp Val Arg Gly 380 Ala Thr Ala Met Ala Ile Leu Ile Al a 400 <210> 333 <211> 1080 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(1057) <223> RXA02158 <400> 333 aacattatcc gtttgacccc gccgctggtg atcaccgacg aaggctattg ccgagacaat cgcataaagg actcaaactt cag Gin gca Ala gag Glu act Thr gg t Gly ggc Gly gat Asp aag Lys tcc Ser t tc Phe tcc Ser gc t Ala aagaaatcgc agacgcagtc atg act tca caa cca Met Thr Ser Gin Pro 1 acc cct gca gag cag Thr Pro Ala Glu Gin gca gcg ccg ttt tcg Ala Ala Pro Phe Ser gtt ctt ttt gat aag Val Leu Phe Asp Lys ggc atc gct cat ttg Gly Ile Ala His Leu tca cag atg ggt aag Ser Gin Met Gly Lys tcc cgc tac gtg gaa Ser Arg Tyr Val Giu 100 115 163 211 259 307 355 403 WO 01/00843 gca att gtg Ala Ile Val gag acg tcC Giu Thr Ser 120 cca tgc cag Pro Cys Gin 135 cct gaa gaa Pro Giu Giu 150 ggc gat ggc Gly Asp Giy acc gcg ggc Thr Ala Gly tgt gcg gaa Arg Ala Giu 200 gcg aag gtt Ala Lys Val 215 gtt gtc atc Vai Val Ile 230 atc gat cgc Ile Asp Arg atg gcg aaa met Ala Lys tac cgt ggc Tyr Arg Gly 280 aaa gtt ttc 1027 Lys Val Phe 295 ctg gtg tgg 1077 Leu Val Trp 310 aac 1080 tgg Trp 105 act Thr att Ile Gly gac Asp a tg Met 185 t tc Phe gtt Vai acc Thr acc Thr gct.

Ala 265 aaa Lys gat Asp c tg Leu cgc Arg g tg Val ctg Leu cca Pro aac Asn 170 gat Asp gtg Val1 gtc Val1 gat Asp aca Thr 250 aac Asn gaa Giu gaa Giu ctg Leu acc tac gca cac agc Thr Tyr Ala His Ser 110 ccg ctg gtg aac tcc Pro Leu Val Asn Ser 125 gct gat ctg cag act Ala Asp Leu Gin Thr 140 gca ggc ctt aag ggt Ala Giy Leu Lys Gly 155 aac atg gcc aac tcc Asn Met Ala Asn Ser 175 att tcc atc atc gct Ile Ser Ilie Ile Ala 190 gag cgc gcg gaa aag Glu Arg Ala Glu Lys 205 acc gac agc ctc gac Thr Asp Ser Leu Asp 220 acc tgg gta tcc atg Thr Trp Val Ser Met 235 cct ttc gtt cct tac Pro Phe Val Pro Tyr 255 gac ggc gcc atc ttc Asp Gly Ala Ile Phe 270 gtg gca gcc tcc gtg Vai Ala Ala Ser Val 285 gca gaa aac cgc ctc Ala Giu Asn Arg Leu 300 gcc aac cag ccg agg Ala Asn Gin Pro Arg 315 aa t Asfl t tg Leu atc Ile aag Lys 160 tac Tyr cc t Pro cgt Arg gag Giu ggt Gly 240 cag Gin ctg Leu att Ile cac t tc Phe tcC Ser gtg Val 145 aag Lys atg Met gaa Glu ggc Gly gtt Val 225 atg Met gtc Val cac His gat Asp gct cac His ga t Asp 130 gaa Giu gct Ala att Ile ggg Gly c ag Gin 210 gcc Ala gaa Giu a ac Asn tgc Cys gga Gly 290 cag gcc Al a 115 gat Asp aac Asn gtg Val 9gc Gly t tc Phe 195 gaa Giu ggc Gly aac Asn gat Asp ctt Leu 275 cca Pro aaa PCTIiBOO/00923 atg gcg 451 Met Ala ctg cac 499 Leu His ctc agc 547 Leu Ser tac ctg 595 Tyr Leu 165 ttt gcc 643 Phe Ala 180 cag cct 691 Gin Pro acc ggc 739 Thr Gly gcc gat 787 Ala Asp gac ggc 835 Asp Gly 245 gag gtc 883 Giu Val 260 cct gcc 931 Pro Ala gcg tcc 979 Ala Ser gca ctg His Ala Gin Lys Ala Leu 305 taagacatgt cccttggctc WO 01/00843 WO 0100843PCT/EBOO/00923 <210> 334 <211> 319 <212> PRT <213> Corynebacterium giutarnicum <400> 334 Met Thr Ser Gin Pro Gin Val Arg His Phe Leu Ala Asp Asp Asp Leu 1 5 10 Thr Pro Ala Giu Gin Ala Glu Val Leu Thr Leu Ala-Ala Lys Leu Lys 25 Ala Ala Pro Phe Ser Glu Arg Pro Leu Glu Gly Pro Lys Ser Val Ala 40 Val Leu Phe Asp Lys Thr Ser Thr Arg Thr Arg Phe Ser Phe Asp Ala 55. Gly Ilie Ala His Leu Gly Gly His Ala Ile Val Val Asp Ser Gly Ser 70 75 Ser Gin Met Gly Lys Gly Giu Ser Leu Gin Asp Thr Ala Ala Val Leu 90 Ser Arg Tyr Val Giu Ala Ilie Val Trp Arg Thr Tyr Ala His Ser Asn 100 105 110 Phe His Ala Met Ala Glu Thr Ser Thr Val Pro Leu Val Asn Ser Leu 115 120 125.

Ser Asp Asp Leu His Pro Cys Gin Ile Leu Ala Asp Leu Gin Thr Ile 130 135 140 Val Glu Asn Leu Ser Pro Glu Giu Gly Pro Ala Gly Leu Lys Gly Lys 145 150 155 160 Lys Ala Val Tyr Leu Gly Asp Gly Asp Asn Asn met Ala Asn Ser Tyr 165 170 175 Met Ile Gly Phe Ala Thr Ala Gly Met Asp Ile Ser Ile Ile Ala Pro 180 185 190 Glu Gly Phe Gin Pro Arg Ala Glu Phe Val Giu Arg Ala Glu Lys Arg 195 200 205 Gly Gin Glu Thr Gly Ala Lys Val Val Val Thr Asp Ser Leu Asp Glu 210 215. 220 Val Ala Gly Ala Asp Val Val Ile Thr Asp Thr Trp Val Ser Met Gly 225 230 235 240 Met Giu Asn Asp Gly Ile Asp Arg Thr Thr Pro Phe Val Pro Tyr Gin 245 250 255 Val Asn Asp Glu Val Met Ala Lys Ala Asn Asp Gly Ala Ile Phe Leu 260 265 270 His Cys Leu Pro Ala Tyr Arg Gly Lys Giu Val Ala Ala Ser Val Ile 275 280 285 WO 01/00843 PCTfIiBO0/00923 Asp Gly Pro Ala Ser Lys Val Phe Asp Glu Ala Glu Asn Arg Leu His 290 295 300 Ala Gin Lys Ala Leu Leu Val Trp, Leu Leu Ala Asn Gin Pro Arg 305 310 315 <210> 335 <211> 1326 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (101)..(1303) <223> RXA02160 <400> 335 ttttcgagga ataccaaccc tttcaacaca ataattttct ttaaacatcc ttgctgtcca ccacggctgg caaggaactt aaaatgaagg agcacacctc atg act aac cgc atc 115 Thr Asn Arg gtt Val ctg Leu ggC Gly gcc Ala gag Giu cag Gin ctc Leu tgc Cys gac Asp ctt Leu aag Lys c ag Gin ggt G ly gag Glu tac Tyr gtt Val1 act Thr ac c Thr 135 ggc Gly gat Asp aac Asn tcC Ser C ca Pro 75 tc t Ser aag Lys aac Asn ctg Leu ggt Gly ggt Gly atg Met atc Ile acc Thr gca Ala cag Gin gac Asp gag Giu 140 c tg Leu gaa Glu gac Asp gtt Val a-tc aag.gca Ilie Lys Ala act Thr gca Al a C gc Arg gca Ala aac Asn 80 cca Pro acc Thr t tc Phe cc t Pro gca Ala tcc Ser cgt Arg gat Asp atg Met atc Ile gt t Val gtc Val1 130 cgt Arg att Ile C tc Leu gca Ala gag Giu tac Tyr gtc Val gca Ala 115 ggc Gly gac Asp cca Pro gac Asp ttg Leu t tc Phe atg Met aag Lys 100 cac His t tc Phe t tc Phe Ile tac Tyr ctg Leu gat Asp gc t Ala aag Lys cac His ggc Gly atg Met gca Ala atc Ile ttc Phe cag Gin 125 atc Ile tcc Ser aac Asn 110 gtt Val att Ile cgc Arg ggt Gly cgt Arg gca Ala tgg acc cgc gac aag gct atc gcc ttc gcc gag gag aac aac gtt cca WO 01/00843 Trp Thr Arg 150 atc gag cag Ile Glu Gin ggc cgc gct Gly Arg Ala acc aag gac Thr Lys Asp 200 cca gat gag Pro Asp Glu 215 gat ggc cgt Asp Gly Arg 230 cgt gca ggc Arg Ala Gly ctc gtg ggc Leu Val Gly gca ctg att Ala Leu Ile 280 gaa ctg gct 1027 Glu Leu Ala 295 gta tac gac 1075 Val Tyr Asp 310 ttc att gat 1123 Phe Ilie Asp ctg cac gca 1171 Leu His Ala ctg tac gac 1219

ASP

too Ser att Ile 185 atc Ile gtc Val cca Pro gca Ala ato Ile 265 aag Lys cgc Arg ggc Gly tcc Ser ggt Gly 345 t to Phe ttc Phe ctg Leu 190 gag Glu gag Glu cag Gin cgo Arg atc Ile 270 ttg Leu gtt Val cot Pro gtc Val1 aat Asn 350 tac Ala tcc Ser 175 gaa Glu gat Asp ggt Gly gc t Ala ott Leu 255 tac Tyr gaa Giu gac Asp otg Leu ac o Thr 335 ggt Gly gac PCTJIBOO/00923 Val Pro 165 gtc tgg 643 Val Trp 180 gct cca 691 Ala Pro aac gct 739 Asn Ala tcc ato 787 Ser Ile aac cgt 835 Asn Arg 245 gac cgt 883 Asp Arg 260 gca ato 931 Ala Ile gag cgc 979 Glu Arg gag gaa Glu Glu gac gog Asp Ala 325 atg gtt Met Val 340 cac too His Ser tto gac Leu Tyr Asp Phe Asn Leu Ala Thr Tyr Asp Thr Gly Asp Thr Phe Asp WO 01/00843 PCTILBOO/00923 cag acc ctg gct aag ggc ttt gtc cag ctg cac ggt ctg tcc tcc aag 1267 Gin Thr Leu Ala Lys Gly Phe Val Gin Leu His Gly Leu Ser Ser Lys 375 380 385 ato gct aac aag cgc gat cgc gaa gct ggc aac aac taagccacct 1313 Ile Ala Asn Lys Arg Asp Arg Glu Ala Gly Asn Asn 390 395 400 tttcaagcat cca 1326 <210> 336 <211> 401 <212> PRT <213> Corynebacterium giutarnicum <400> 336 Met Thr Asn Arg Ile Val Leu Aia Tyr 1 Val1 Val1 Gin Lys Gly Leu His Giu Ala 145 Giu Asp Leu Ala Ala Ser Arg Asp Met Ilie Val Vali 130 Arg Asn Gin Trp Leu 210 Lys Giy Ala Tyr Pro Giu Gly 120 Asp Arg Gin Aia Asp 200 Glu Met Gly Ala Cys Leu Ala 105 Lys Pro Asp Ser Ile 185 Ile Val Ser Ile Glu Giu Leu Vali Gly Giy Asn Lys Val 170 Giu Tyr Leu Glu Asp Val Ile Ile Phe Gin 125 Ile Ala Pro Tyr Thr 205 Thr Ala Arg Aia Asn Pro Thr Phe Pro G iu 160 Ile Asp Pro Ile Ile Ser 220 Phe Glu Giy Giy WO 01/00843 WO 0100843PCTfIiBOO/00923 Lys 225 Giu Met Ala Val Arg 305 Arg Asp Arg Gly Gly 385 Asn Pro Val Ser Ile Giu Val Pro Thr 290 Trp Ser Ile Ser Asp 370 Leu Leu Giu Gly 275 Ile Ala Leu Arg Ser 355 Thr Ser Asn Asp 260 Ala Giu Giu Asp Met 340 His Phe Ser Arg 245 Arg Ile Arg Glu Ala 325 Val Ser Asp Lys Asp 230 Arg Leu Ala Glu Val1 310 Phe Leu Leu Gin Ile 390 Ala Val Leu Leu 295 Tyr Ile His Tyr Thr 375 Ala Gly Gly Ile .280 Ala Asp Asp Ala Asp 360 Leu Asn Ala Ile 265 Lys Arg Gly Ser Gly 345 Phe Ala Lys Gly Arg Pro Val Ser Val Leu Gin Ala Ile 235 240 Gly Ser His Lys Trp 315 Gin Ile Leu G ly Asp 395 Val Arg Giu Arg 300 Phe Giu Thr Ala Phe 380 Arg Gly Giu Ala 285 Gly Giy His Ile Thr 365 Val Glu Asp Giu Asp Ala Lys 320 Gly Arg Thr His Asn 400 <210> 337 <211> 1554 <212> DNA <213> Corynebacterium giutamicun <22 0> <221> CDS <222> (101) .(1531) <223> RXN02162 <400> 337 gatcgctaac aagcgcgatc gcgaagctgg caacaactaa gccacctttt caagcatcca gactagaact tcaagtattt agaaagtaga agaacaccac atg gaa cag cac gga Met Giu Gin His Giy 1 acc aat gaa ggt gcg ctg tgg ggc ggc cgc ttc tcc ggt gga ccc tcc Thr Asn Giu Giy Ala Leu Trp Gly Gly Arg Phe Ser Gly Gly Pro Ser 15 gag gcc atg ttc gcc ttg agt gtc tcc act cat ttc gac tgg gtt ttg Giu Ala Met Phe Ala Leu Ser Val Ser Thr His Phe Asp Trp Vai Leu WO 01/00843 WO 0100843PCTIIBOO/00923 gcc cct tat Ala Pro Tyr gat gtg ttg gcc tcc aag gca cac gcc Asp Val Leu Ala Ser Lys Ala His Ala gtt ttg cac Val Leu His caa gca Gln Ala gat cta ctt tct Asp Leu Leu Ser gaa gat cta gcc Glu Asp Leu Ala atg ctg gct ggg Met Leu Ala Gly gat cag ctg ggc Asp Gln Leu Gly gat gtc gcc gac Asp Val Ala Asp gga Gly acc ttc ggt ccg Thr Phe Gly Pro cct tct gat gag Pro Ser Asp Glu gtg cac ggc gcg Val His Gly Ala gaa cgc ggt gtg Glu Arg Gly Val att gac Ile Asp 100 cgc gtt ggt Arg Val Gly aac gac cag Asn Asp Gln 120 cgc gac.atc Arg Asp Ile 135 cc t Pro 105 gag gtg ggc ggc Glu Val Gly Gly ctg cgc gct ggt Leu Arg Ala Gly cgt tcc cgc Arg Ser Arg 115 gac gca gtg Asp Ala Val gtg gca acc ctg Val Ala Thr Leu cgc atg tgg gtc Arg Met Trp Val gcg ctg gga Ala Leu Gly acc gag ctt gtc Thr Glu Leu Val gac Asp 145 gcc ctc agc gcc Ala Leu Ser Ala caa Gln 150 gct aag gca cat Ala Lys Ala His ggc gcg atc atg Gly Ala Ile Met cca Pro 160 ggc aag acc cac Gly Lys Thr His cag gca gct cag Gln Ala Ala Gln gtc ctt ctg gca Val Leu Leu Ala cag ctg ctg gca Gln Leu Leu Ala cac gca His Ala 180 cag cct ttg Gln Pro Leu ctt gcg gtg Leu Ala Val 200 cgc gat att gat Arg Asp Ile Asp atc cgt gac ctg Ile Arg Asp Leu gac aag cgt Asp Lys Arg 195 tcc tct ttg Ser Ser Leu tct cct tac ggt Ser Pro Tyr Gly ggc gca ctt gct Gly Ala Leu Ala ggt Gly 210 aag ctc Lys Leu 215 aac cct gaa gca Asn Pro Glu Ala gct gaa gaa ctc Ala Glu Glu Leu 9gc Gly 225 ttt gat tcc gca Phe Asp Ser Ala gca Al a 230 gat aac tcc att Asp Asn Ser Ile gcc acc agc tcc Ala Thr Ser Ser gat ttc gca tct Asp Phe Ala Ser acc gcc ttc gtg Thr Ala Phe Val gcg cag ctt gca Ala Gln Leu Ala gat atg tcc cgc Asp Met Ser Arg ttg gct Leu Ala 260 gaa gaa atc Glu Glu Ile atc I le 265 gca tgg tgc acc Ala Trp Cys Thr gaa ttt ggt tac Glu Phe Gly Tyr atc acc ttg Ile Thr Leu 275 WO 01/00843 tct gat tc Ser Asp Ser 280 PCTIBOOIOO923 cct gac 1027 Pro Asp 295 aac ctc 1075 Asn Leu 310 aac cgc 1123 Asn Arg cag ctc 1171 Gin Leu acc ttc 1219 Thr Phe ctt gcc 1267 Leu Ala 375 cgt gag 1315 Arg Giu 390 agg gga 1363 Arg Gly gat gca 1411 Asp Ala gtg Val acc Thr tgg tcc aca ggc Trp Ser Thr Gly gca gag ctg acc Ala Glu Leu Thr 300 ggt ctg ctg gct Giv Leu Leu Ala tca atc atg Ser Ile Met ggc aag tct Gly Lys Ser ctg aag gca Leu Lys Ala acc Thr ccg cag aag aag aac 979 Pro Gin Lys Lys Asn 290 ggt cgc ttg atc ggt Gly Arg Leu Ile Gly 305 cag cct tta gcg tac Gin Pro Leu Aia Tyr 325 gta gat tcc gtg gcg Val Asp Ser Val Ala 340 ttg gtt tcc acc ttg Leu Vai Ser Thr Leu gac ctg cag Asp Leu Gin 315 320 aac ctg Asn Leu 345 aac acc Asn Thr 330 ctg Leu gag Glu gaa gat aag gaa Giu Asp Lys Giu ctc cct gca atg Leu Pro Ala Met 350 cgc atg cgt gaa Arg Met Arg Glu cca Pro 335 act Thr atc Ile ggt Gly .355 cU: gca cca gca ggt ttc acc Leu Ala Pro Aia Giy Phe Thr 370.

gtg cgc cag ggc gtt cca ttc Vai Arg Gin Gly Val Pro Phe 365 gac ttg gct gag Asp Leu Aia Glu 380 tgg atg Trp Met gca cac gaa gca Ala His Giu Ala 395 gtg gac ctt atc Val Asp Leu Ile 410 cgt ctg acc cca Arg Leu Thr Pro 425 tC Ser gat Asp ggc gct tgc gtg Gly Ala Cys Val 400 ctc act gat gaa Leu Thr Asp Glu 385 cgg atc gcg gag tcc Arg Ile Ala Giu Ser 405 gaa ctc agt ggc gtt Glu Leu Ser Giy Val 420 ctc acc att gat ggt Leu Thr Ile Asp Giy 415 gag gta cgg gaa Giu Val Arg Giu gtg Vali gca gtg gct 1459 Ala Val Ala 440 gcg gag caa 1507 tcc cgt gca acg cgc Ser Arg Aia Thr Arg 445 cgc gca cgt gtc gat 430 435 ggt gga acc gcg ggc gtg cgg gtt Gly Giy Thr Ala Giy Val Arg Val 450 gcc gca agt acc gct cac gcg gag Ala Glu Gin Arg Ala Arg Val Asp Ala Ala Ser Thr Ala His Aia Giu 455 460 465 WO 01/00843 tgg gca cgt gcg ggg gta cgt cga taagcattag tttatggcct gig 1554 Ti-p Ala Arg Ala Gly Val Arg Arg 470 475 <210> 338 <211> 477 <212> PRT <213> Corynebacterium glutanicun PCTIiBOO/00923 <400> 338 Met Giu Gin His 1 Ser Phe Ala Thr Thr Arg Ala Val Asp 145 Gly Leu Asp Ala Gly 225 Asp Met Gly Asp Lys Met Phe Gly Gly Arg 130 Ala Lys Leu Leu Gly 210 Phe Phe Ser Gly Ti-p Val Leu Gly Val Arg 115 Asp Leu Thr Ala Asp 195 Ser Asp Ala Arg Pro Val Leu Ala Pro Ile 100 Ser Ala Ser His His 180 Lys Ser Ser Ser Lau G ly 5 Ser Leu His Gly Leu Asp Arg Val Ala Phe 165 Ala Arg Leu, Ala Glu 245 Ala Thr Asn Glu Gly Glu Ala Met Phe 25 Ala Pro Tyr Asp 40 Gin Ala Asp Leu 55 Leu Asp Gin Leu 70 Pro Ser Asp Glu Arg Val Gly Pro 105 Asn Asp Gin Val 120 Arg Asp Ile Ala 135 Gin Ala Lys Ala 150 Gin Ala Ala Gin Gin Pro Leu Leu 185 Leu Ala Val Ser 200 Lys Leu Asn Pro 215 Ala Asp Asn Ser 230 Thr Ala Phe Val Giu Gu Ile Ile Ala 10 Ala Val1 Leu G ly Asp 90 Giu Ala Leu His Pro 170 Arg Pro Giu Ile Leu 250 Ala Leu Leu.

Leu Ser Lys 75 Val Val Thr Gly Ala 155 Val Asp Tyr Ala Asp 235 Ala Ti-p Ti-p Ser Ala Asp Asp His Gly Leu Thr 140 Gly Leu Ile Gly Ile 220 Ala Gin Cys Gly Val Ser Giu Val1 Gly Gly Phe 125 Thr Ala Leu Asp Ser 205 Ala Thi- Leu Thr Gly Ser Lys Asp Ala Ala Arg 110 Arg Glu Ile Ala Arg 190 Gly Giu Ser Ala Pro Arg Thr Ala Lau Asp Met Leu Met Leu Met His 175 Ile Ala Glu Ser Val 255 Glu Phe His His Ala Gly Giu Arg Ti-p Val1 Pro 160 Gin Arg Leu Leu Arg 240 Asp Phe WO 01/00843 WO 0100843PCT/IBOO/00923 260 265 270 Gly Tyr Ile Thr Leu Ser Asp Ser Trp Ser Thr Gly Ser Ser Ilie Met 275 280 285 Pro Gin Lys Lys Asn Pro Asp Val Ala Giu Leu Thr Arg Giy Lys Ser 290 295 300 Giy Arg Leu Ilie Gly Asn Leu Thr Gly Leu Leu Ala Thr Leu Lys Ala 305 310 315 320 Gin Pro Leu Ala Tyr Asn Arg Asp Leu Gin Giu Asp Lys Giu Pro Ile 325 330 335 Val Asp Ser Val Aia Gin Leu Asn Leu Leu Leu Pro Ala Met Thr Gly 340 345 350 Leu Vai Ser Thr Leu Thr Phe Asn Thr Glu Arg Met Arg Giu Leu Ala 355 360 365 Pro Ala Gly Phe Thr Leu Aia Thr Asp Leu Ala Giu Trp Met Val Arg 370 375 380 Gin Gly Val Pro Phe Arg Giu Ala His Giu Ala Ser Gly Ala Cys Val 385 390 395 400 Arg le. Ala Glu Ser Arg Gly Val Asp Leu Ile Asp Leu Thr Asp Giu 405 410 415 Giu Leu Ser Gly Val Asp Ala Arg Leu Thr Pro Giu Vai Arg Giu Val 420 425 430 Leu Thr Ilie Asp Gly Ala Val Ala Ser Arg Ala Thr Arg Gly Giy Thr 435 440 445 Ala Gly Val Arg Val Ala Giu Gin Arg Ala Arg Val Asp Ala Ala Ser 450 455 460 Thr Ala His Ala Giu Trp, Ala Arg Ala Gly Val Arg Arg 465 470 475 <210> 339 <211> 906 <212> DNA <213> Corynebacterium glutamicum <22 0> <221> CDS <222> (101)..(883) <223> FRXA02161 <400> 339 gatcgctaac aagcgcgatc gcgaagctgg caacaactaa gccacctttt caagcatcca gactagaact tcaagtattt agaaagtaga agaacaccac atg gaa cag cac gga 115 Met Giu Gin His Gly 1 acc aat gaa ggt gcg ctg tgg ggc ggc cgc ttc tcc ggt gga ccc tcc 163 Thr Asn Giu Gly Ala Leu Trp Gly Gly Arg Phe Ser Gly Gly Pro Ser WO 01/00843 WO 0100843PCTIiBOO0923 gag gcc atg Glu Ala Met gcc cct tat Ala Pro Tyr ttc Phe gcc ttg agt gtc Ala Leu Ser Val act cat ttc gac Thr His Phe Asp 2 0 tgg gtt ttg Trp Vai Leu gtt ttg cac Val Leu His gat gtg ttg gcc tcc aag gca cac gcc Asp Val Leu Ala Ser Lys Ala His Ala caa gca Gin Ala gat cta ctt tct gat gaa gat cta gcc ASP Leu Leu Ser Asp Glu Asp Leu Ala atg ctg gct ggg Met Leu Ala Giy 307 355 ctt Leu gat cag ctg ggc Asp Gin Leu Gly aag gat gtc gcc gac gga acc ttc ggt ccg ctg Lys Asp Val Ala Asp Gly Thr Phe Gly Pro Leu 75 80 cct tct gat gag Pro Ser Asp Glu gat Asp gtg cac ggc gcg Val His Gly Ala gaa. cgc ggt gtg Glu Arg Gly Val att gac Ile Asp 100 cgc gtt ggt Arg Val Gly aac gac cag Asn Asp Gln 120 gag gtg ggc ggc Glu Val Gly Gly ctg cgc gct ggt Leu Arg Ala Giy cgt tcc cgc Arg Ser Arg 115 gac gca. gtg Asp Ala Val gtg gca acc ctg ttc cgc atg tgg gtc Vai Ala Thr Leu Phe Arg Met Trp Vai 125 499 cgc gac Arg ASP 135 atc gcg ctg gga aca acc gag ctt gtc Ile Ala Leu Gly Thr Thr Glu Leu Val 140 gcc ctc agc gcc Ala Leu Ser Ala gct aag gca cat Ala Lys Ala His gca Ala 155 ggc gcg atc atg Gly Ala Ile Met ggc aag acc cac Gly Lys Thr His cag gca gct cag Gin Ala Ala Gin ccg Pro 170 gtc ctt ctg gca Val Leu Leu Ala cag ctg ctg gca Gin Leu Leu Ala cac gca His Ala 180 cag cct ttg Gin Pro Leu ctt gcg gtg Leu Ala Val 200 cgc gat att gat Arg Asp Ile Asp atc cgt gac ctg Ile Arg Asp Leu gac aag cgt Asp Lys Arg 195 tcc tct ttg Ser Ser Leu tct cct tac ggt Ser Pro Tyr Gly ggc gca ctt gct Gly Ala Leu Ala aag ctc Lys Leu 215 aac cct gaa gca Asn Pro Giu Ala gct gaa gaa ctc Ala Glu Giu Leu ttt gat tcc gca Phe Asp Ser Ala gat aac tcc att Asp Asn Ser Ilie gat Asp 235 gcc acc agc: tcc Ala Thr Ser Ser gat ttc gca tct Asp Phe Ala Ser acc gcc ttc gtg Thr Ala Phe Val ctg Leu 250 gcg cag ctt gca Ala Gln Leu Ala gga tat gtc ccg Gly Tyr Val Pro ctt ggc Leu Giy 260 WO 01/00843 PCT/IB00/00923 tgaagaaatc atcgcatggt gca 906 <210> 340 <211> 261 <212> PRT <213> Corynebacterium glutamicum <400> 340 Met Glu Gin His Gly Thr Asn Glu Gly Ala Leu Trp Gly Gly Arg Phe 1 5 10 Ser Gly Gly Pro Ser Glu Ala Met Phe Ala Leu Ser Val Ser Thr His 25 Phe Asp Trp Val Leu Ala Pro Tyr Asp Val Leu Ala Ser Lys Ala His 40 Ala Lys Val Leu His Gin Ala Asp Leu Leu Ser Asp Glu Asp Leu Ala 55 Thr Met Leu Ala Gly Leu Asp Gin Leu Gly Lys Asp Val Ala Asp Gly 70 75 Thr Phe Gly Pro Leu Pro Ser Asp Glu Asp Val His Gly Ala Met Glu 90 Arg Gly Val Ile Asp Arg Val Gly Pro Glu Val Gly Gly Arg Leu Arg 100 105 110 Ala Gly Arg Ser Arg Asn Asp Gin Val Ala Thr Leu Phe Arg Met Trp 115 120 125 Val Arg Asp Ala Val Arg Asp Ile Ala Leu Gly Thr Thr Glu Leu Val 130 135 140 Asp Ala Leu Ser Ala Gin Ala Lys Ala His Ala Gly Ala Ile Met Pro 145 150 155 160 Gly Lys Thr His Phe Gin Ala Ala Gin Pro Val Leu Leu Ala His Gin 165 170 175 Leu Leu Ala His Ala Gin Pro Leu Leu Arg Asp Ile Asp Arg Ile Arg 180 185 190 Asp Leu Asp Lys Arg Leu Ala Val Ser Pro Tyr Gly Ser Gly Ala Leu 195 200 205 Ala Gly Ser Ser Leu Lys Leu Asn Pro Glu Ala Ile Ala Glu Glu Leu 210 215 220 Gly Phe Asp Ser Ala Ala Asp Asn Ser Ile Asp Ala Thr Ser Ser Arg 225 230 235 240 Asp Phe Ala Ser Glu Thr Ala Phe Val Leu Ala Gin Leu Ala Xaa Gly 245 250 255 Tyr Val Pro Leu Gly 260 WO 01/00843 WO 0100843PCT(1B00100923 <210> <211> <212> <213> <220> <221> <222> <223> 341 786

DNA

Corynebacterium glutamicum

CDS

(101)..(763) FRXA02 162 <400> 341 ctcggctttg attccgcagc agataactcc attgatgcca ccagctcccg cgatttcgca tctgaaaccg ccttcgtgct ggcgcagctt gcangtggat atg tcc cgc ttg Met Ser Arg Leu gaa gaa atc atc Giu Giu Ile Ile gca tgg tgc acc cca gaa ttt ggt tac atc acc ttg Ala Ti-p Cys Thr Pro Giu Phe Gly Tyr Ile Thr Leu 15 tct gat tcc Ser Asp Ser cct gac gtg Pro Asp Val tcc aca ggc agc Ser Thr Gly Ser atc atg ccg cag Ile Met Pro Gin aag aag aac Lys Lys Asn ttg atc ggt Leu Ile Gly gca gag ctg acc Ala Giu Leu Thr ggc aag tct ggt Gly Lys Ser Gly aac ctc Asn Leu acc ggt ctg ctg Thr Gly Leu Leu acc ctg aag gca Thi- Leu Lys Ala cag Gin cct tta gcg tac Pro Leu Ala Tyr cgc gac ctg cag Arg Asp Leu Gin gaa gat aag gaa cca atc gta gat tcc gtg gcg Giu Asp Lys Giu Pro Ile Val Asp Ser Vai Ala 80 cag ctc aac ctg Gin Leu Asn Leu ctc cct gca atg Leu Pro Ala Met ggt ttg gtt tcc Gly Leu Val Ser acc ttg Thi- Leu 100 acc ttc aac Thr Phe Asn ctt gcc acc Leu Ala Thr 120 gag cgc atg cgt Giu Arg Met Arg ctt gca cca gca Leu Ala Pro Ala ggt ttc acc Gly Phe Thr 115 gtt cca ttc Val Pro Phe gac ttg gct gag Asp Leu Ala Giu atg gtg cgc cag Met Vai Arg Gin cgt gag Arg Giu 135 gca cac gaa gca Ala His Glu Ala ggc gct tgc gtg Gly Ala Cys Vai Cgg Arg 145 atc gcg gag tcc Ile Ala Giu Ser gga gtg gac ctt Gly Val Asp Leu atc Ile 155 gat ctc act gat Asp Leu Thr Asp gaa Giu 160 gaa ctc agt ggc Giu Leu Ser Gly gat gca cgt ctg Asp Ala Arg Leu acc Thr 170 cca gag gta cgg Pro Giu Val Arg gtg ctc acc att Val Leu Thr Ile gat ggt Asp Gly 180 WO 01/00843PCIBO/92 PCT/EBOO/00923 gca gtg gct tcc cgt gca acg cgc ggt Ala Val Ala Ser Arg Ala Thr Arg Gly 185 190 gcg gag caa cgc gca cgt gtc gat gcc Ala Glu Gln Arg Ala Arg Val Asp Ala 200 205 tgg gca cgt gcg ggg gta cgt cga taa~ Trp Ala Arg Ala Gly Val Arg Arg 215 220 <210> 342 <211> 221 <212> PRT <213> Corynebacteriun glutarnicum <400> 342 Met Ser Arg Leu Ala Glu Glu Ile Ile gga acc gcg ggc gtg cgg gtt Gly Thr Ala Gly Val Arg Val 195 gca agt acc gct cac gcg gag Ala Ser Thr Ala His Ala Glu 210 Icattag tttatggcct gtg 1 Gly Pro Gly Gin Val1 Leu Pro Gln Arg 145 Glu Leu Ala Thr Tyr Gln Arg Pro Asp Val Ala Gly 130 I le Leu Thr Gly Ala 210 5 Leu Asn Gly Tyr Ala Leu Thr Phe Ser Val 165 Gly Val Glu Ser Pro Asn Asn 70 Gln Thr Leu Arg Arg 150 Asp Ala Ala Trp Asp Asp Leu 55 Arg Leu Phe Ala Glu 135 Gly Ala Val Glu Ala 215 Trp Ala Gly Leu Leu Thr 105 Asp His Asp Leu Ser 185 Arg Ala Ala 10 Ser Glu Leu Gin Leu 90 Glu Leu Glu Leu Thr 170 Arg Ala Gly Trp Thr Leu Leu Glu 75 Leu Arg Ala Ala Ile 155 Pro Ala Arg Val Cys Gly Thr Ala Asp Pro Met Glu Ser 140 Asp Glu Thr Val Arg 220 Pro Ser Gly Leu Glu Met Glu 110 Met Ala Thr Arg Gly 190 Ala Glu Ile Lys Lys Pro Thr Leu Val Cys Asp Glu 175 G ly Ala Phe Met Ser Ala Ile Gly Ala Arg Val G lu 160 Val Thr Ser WO 01/00843 WO 0100843PCT[IBOO/00923 <210> 343 <211> 1269 <212> DNA <213> Corynebacterium glutamicun <220> <221> <222> <223>

CDS

(101)..(1246) RXA02262 <400> 343 gcaccaattt cggacctgaa atccccgagg aaaccgtgcc cgacgccgtg caggtgggcg tcgataagca aaaaatcgct gatactcgaa aggcctcaaa atg acc gca acc Met Thr Ala Thr acc act gaa acc gcc atc aat ttc ttg Thr Thr Glu Thr Ala Ile Asn Phe Leu ttg agc gaa ccg Leu Ser Glu Pro gac atg Asp Met atc gcg gcc Ile Ala Ala gaa acg ctc Glu Thr Leu gtc aaa gac gtc Val Lys Asp Val caa tgc gtc gat Gin Cys Val Asp gtc atg gag Val Met Glu gcc ggt ttg Ala Gly Leu gtg ctc ttg gcg Val Leu Leu Ala ggc gac tac aaa Gly Asp Tyr Lys atg Met aac tcc Asn Ser aac tcg cat ggc Asn Ser His Gly atg atc acc ttc Met Ile Thr Phe gaa aac cca gaa Glu Asn Pro Glu gaa ggc atg ccc Glu Gly Met Pro aag Lys gac ggc ccc gac Asp Gly Pro Asp cga ttc atg gcg Arg Phe Met Ala ccc gca tac ctc Pro Ala Tyr Leu ggg cga ttc aaa Gly Arg Phe Lys acc ggc gtg aag Thr Gly Val Lys tgg tac Trp Tyr 100 gga tcc aac Gly Ser Asn acc ttc gtc Thr Phe Val 120 gcg gaa Ala Glu 105 aac aag gcc Asn Lys Ala ggc ttg cct cgc Gly Leu Pro Arg tcg atc cac Ser Ile His 115 gcc atc atg Ala Ile Met ctc aac gac acg Leu Asn Asp Thr acc ggt gca ccg Thr Gly Ala Pro aag Lys 130 tcc gcg Ser Ala 135 aac ctg ctg tcc Asn Leu Leu Ser gcc Ala 140 tac cgc acc ggc Tyr Arg Thr Gly gtt ccc ggc gtg Val Pro Gly Val ggc Gly 150 gtg aag cac tta Val Lys His Leu gtc gcc gac gcg Val Ala Asp Ala acc ttg gct gtc gtc Thr Leu Ala Val Val 165 gga cct ggt gtc atg gcg aaa acc atc acc gaa gcg tgc atc gca gag Gly Pro Gly Val met Ala Lys Thr Ile Thr Glu Ala Cys Ile Ala Glu WO 01/00843 cgc cca Arg Pro atc aac Ile Asn gtg gtc Val Val 215 gtc atc Val Ile 230 tac ttc Tyr Phe gcc gcc Ala Ala gtt gtt Val Val cca cag 1027 Pro Gin 295 ctg gcg 1075 Leu Ala 310 gat atc 1123 Asp Ile atc ctc 1171 Ile Leu acc caa 1219 Thr Gin aac ctg 1266 Asn Leu 375 atc Ile 185 ttt Phe gtc Val gcc Ala aaa Lys cgc Arg 265 tac Tyr tac Tyr caa Gin tcc Ser tcc Ser 345 tat Tyr gaa Glu 170 ac c Thr gca Ala gga Gly acc Thr gaa Giu 250 t tc Phe atg Met caa Gin gga Gly ggc Gly 330 gtc Val1 gaa Glu tca Ser acc Thr aca Thr tc t Ser acc Thr 235 tgg Trp, gac Asp ggg Gly cta Leu aaa Lys 315 aag Lys ggC Gly aac Asn ccc Pro atc aag atc Ile Lys Ile 190 tgg gcg ttg Trp Ala Leu 205 gaa gaa gac Giu Glu Asp 220 acg gac gcc Thr Asp Ala ctc aag ccg Leu Lys Pro gac gct tat Asp Ala Tyr 270 ctc tac gaa Leu Tyr Glu 285 ctc ggc att Leu Gly Ile 300 ctc gac ctt Leu Asp Leu cta ccc gga Leu Pro Gly ggc atg cca Gly Met Pro 350 gcc ctg gaa Ala Leu Glu 365 gca ctg gct Ala Leu Ala 380 175 aag Lys gaa Giu gtg Val1 gcc Ala ggc Gly 255 t tg Leu gcc Ala cca Pro gca Al a cgc Arg 335 gta Val aaa gga Gly aaa Lys gtc Val ggc Gly 240 gca Ala ctt Leu tgg Trp gga Gly aag Lys 320 acc Thr gaa Glu ggc cgc Arg ttc Phe aaa Lys 225 tcc Ser ttg Leu gac Asp gca Ala acc Thr 305 att Ile aac Asn gac Asp gtc agc Ser ccc Pro 210 gac Asp tcc Ser c tg Leu gac Asp gaa Glu 290 cac His tcc Ser gat Asp gtc Val ggc gaa Glu 195 gag Glu gcc Ala gcc Ala ctg Leu gcc Ala 275 gaa Glu tgg Trp cag Gin gag Giu gcc Ala 355 acc PCTIIBOO/00923 180 cgc ggc 691 Arg Gly atc gaa 739 Ile Glu gac atc 787 Asp Ile ttc cca 835 Phe Pro 245 ctt cca 883 Leu Pro 260 cgc ctc 931 Arg Leu tac ggc 979 Tyr Gly tac gac.

Tyr Asp att ggc Ile Gly 325 gaa atc Glu Ile 340 tgg gca Trp Ala aca ttg Lys Gly Val Gly Thr Thr Leu 370 tgagagaaga aacaacaatg WO 01/00843 PCTIIBOO/00923 aaa 1269 <210> 344 <211> 382 <212> PRT <213> Corynebacteriun giutamicum <400> 344 Met Thr Ala Thr Tyr Thr Thr Giu Thr Ala Ile Asn Phe Leu Phe Leu 1 5 10 Ser Giu Pro Asp Met Ile Ala Ala Gly Val Lys Asp Vai Ala Gin Cys 25 Val Asp Val Met Glu Glu Thr Leu Val Leu Leu Ala Gin Gly Asp Tyr 40 Lys Met Ala Gly Leu Asn Ser Asn Ser His Gly Ala Met Ile Thr Phe 55 Pro Glu Asn Pro Glu Phe Glu Gly Met Pro Lys Asp Gly Pro Asp Arg 70 75 Arg Phe Met Aia Met Pro Ala Tyr Leu Gly Gly Arg Phe Lys Asn Thr 90 Gly Val Lys Trp Tyr Gly Ser Asn Ala Glu Asn Lys Ala Ser Gly Leu 100 *105 110 Pro Arg Ser Ilie His Thr Phe Val'Leu Asn Asp Thr Val Thr Gly Ala 115 120 125 Pro Lys Ala Ile Met Ser Ala Asn Leu Leu Ser Ala Tyr Arg Thr Gly 130 135 140 Ala Val Pro Gly Val Gly Val Lys His Leu Ala Val Ala Asp Ala Thr 145 150 155 160 Thr Leu Ala Val Val Gly Pro Gly Val Met Ala Lys Thr Ile Thr Glu 165 170 175 Ala Cys Ile Ala Glu Arg Pro Gly Ile Thr Thr Ile Lys Ile Lys Gly 180 185 190 Arg Ser Glu Arg Gly Ile Asn Ala Phe Ala Thr Trp Ala Leu Glu Lys 195 200 205 Phe Pro Glu Ilie Giu Val Val Ala Val Gly Ser Glu Glu Asp Val Val 210 215 220 Lys Asp Ala Asp Ile Val Ile Ala Ala Thr Thr Thr Asp Ala Ala Gly 225 230 235 240 Ser Ser Ala Phe Pro Tyr Phe Lys Lys Glu Trp Leu Lys Pro Gly Ala 245 250 255 Leu Leu Leu Leu Pro Ala Ala Gly Arg Phe Asp Asp Ala Tyr Leu Leu 260 265 270 WO 01/00843 ASP ASP Al 27 Ala Glu GI 290 Thr His Tr 305 Ile Ser Gl Asn Asp Gi Asp Val Al Val Gly Th 370 Val1 Pro Leu 310 Asp Ile Thr Asn Val Gin 295 Ala Ile Leu Gin Leu 375 Gly Leu Lys 315 Lys Gly Asn Pro Leu Leu 300 Leu Leu Gly Ala Ala 380 Glu Ile Leu Gly Pro 350 Gl u Ala PCTJIB00100923 Ala Trp Pro Gly Ala Lys 320 Arg Thr 335 Val Giu Lys Gly <210> 345 <211> 1065 <212> DNA <213> Corynebacterium glutaxnicum <22 0> <221> CDS <222> (101)..(1042) <223> RXA00219 <400> 345 tttgccgtac atgcgcgagc acctcctcaa cagcccgcac cacaagcact aaaacagcat taaagaaaga aagctttttc aac Asn ggc Gly tac Tyr cat His tgg Tip tcc Ser acg tcc Thr Ser acc tat Thr Tyr acc acc Thr Thr att gtc Ile Val atc gct Ile Ala aag ctg Lys Leu gat caa Asp Gln gag ggt Glu Giy gat ggc Asp Gly ctg ggg Leu Gly acc ggc Thr Gly cgg att Arg Ile tcc Ser tcc Ser atc Ile 45 cag Gin gcg Ala c tc Leu caa gct Gin Ala 15 gtc atc Val Ile 30 agc att Ser Ile gca ctg Ala Leu ttc atc Phe Ile ggc ggc Giy Gly caccgaccaa tcacataaga gtg gcc cgt aag aaa Val Ala Arg Lys Lys 1 aac acg ccc att gct Asn Thr Pro Ile Ala ttg gag gcc gat tcc Leu Giu Ala Asp Ser ggc gtg ccc agc tct Gly Val Pro Ser Ser ttt gag tac atg cgg Phe Glu Tyr Met Arg gcg cat cag gat gca Ala His Gin Asp Ala gcg tgc acg atg gcc Ala Cys Thr Met Ala WO 01/00843 WO 0100843PCTTfiBOO/00923 agg tat ttc Arg Tyr Phe ttg gat gca Leu Asp Ala 120 gcg Ala 105 gat gtt tac ccg cag tca cgc aac act Asp Val Tyr Pro Gin Ser Arg Asn Thr 110 100 gtc gtg gaa Val Val Giu 115 gac att ccg Asp Ile Pro gag ctt gcc cgc Glu Leu Ala Arg ctg Leu 125 tcg cgt gaa tgg Ser Arg Glu Trp t tc Phe 130 cgc gcg Arg Ala 135 cca cgg gta aag Pro Arg Val Lys att Ile 140 cgt gtg gat gat Arg Val Asp Asp cga atg gtg gca Arg Met Val Ala gaa Glu 150 tct ttc act ccc Ser Phe Thr Pro gca Ala 155 agc cgc gat gtg Ser Arg Asp Val atc cgt gac gtt Ile Arg Asp Val ttt Phe 165 gcc gga gct atc Ala Gly Ala Ile acg Thr 170 ccg cag aac ttc Pro Gin Asn Phe acc Thr 175 acc gtg gag ttc Thr Val Glu Phe ttt gag Phe Glu 180 cac tgt cac His Cys His ggc gat cat Gly Asp His 200 ggc ctt gct ccc Gly Leu Ala Pro ggc Gly 190 gga ttg tac gtt Gly Leu Tyr Val gcc aac tgt Ala Asn Cys 195 gcg gga atg Ala Gly Met tcg gat ctg cgc Ser Asp Leu Arg gga G ly 205 gct aaa tct gag Ala Lys Ser Glu atg gag gtg ttc gag cac Met Glu Val Phe Glu His gcg gtc atc gcc Ala Val Ile Ala ccc ccg atg ctt Pro Pro Met Leu aaa Lys 230 ggg cgc cgt tac Gly Arg Arg Tyr ggc Gly 235 aac atc att Asn Ile Ile ttg atg ggt tca gac acc gag Leu Met Gly Ser Asp Thr Glu .240 245 ttc ttt agc tcc Phe Phe Ser Ser agc acg gaa gcg Ser Thr Giu Ala tcc Ser 255 gcg att acc cgt Ala Ile Thr Arg gag ctt Giu Leu 260 ctt ggc ggc Leu Gly Gly aaa ttc gcc Lys Phe Ala 280 gtt cca gcg cag Val Pro Ala Gin aag gat gaa tcc Lys Asp Glu Ser tgg gtg cgg Trp Val Arg 275 tct acc ctc Ser Thr Leu tcg gga gcc cag Ser Gly Ala Gin cgc cac gat ggg Arg His Asp Gly caa atg ccg agt gat act cca caa cac cct gcg gaa acg ccg gag cat 1027 Gin Met 295 tca aac 1065 Ser Asn 310 Pro Ser Asp Thr Gin His Pro Ala Giu 305 Thr Pro Giu His aca cag cca taaaaaattc cgctggcgcg tcc Thr Gin Pro WO 01/00843 <210> 346 <211> 314 <212> PRT <213> Corynebacteriun glutamicun <400> 346 Val Ala Arg Lys Lys Asn Thr Ser Asp PCT/IBOO/00923 1 Asn Leu Gly Phe Ala Ala Asn Trp Ala 145 Ile Val1 Tyr Giu Asp 225 Gly Ile Glu Thr Giu Val1 Giu His Cys Thr Phe 130 Arg Arg Glu Val1 Leu 210 Pro Ser Thr Ser Pro Ala Pro Tyr Gin Thr Val 115 Asp Met Asp Phe Ala 195 Al a Pro Asp Arg Trp 275 Tyr Thr 40 Val1 Ala Leu Phe Ala 120 Pro Phe Ala His His 200 Val Arg Ser Gly Ala 280 Glu Asp Leu Thr Arg Ala 105 Glu Arg Thr Ile Arg 185 Ser Phe Arg Ser G ly 265 Ser Gin Gly Gly Gly Gly Ile Asp Leu Val1 Pro Thr 170 Gly Asp Glu Tyr Asn 250 Val Gly Arg Tyr Leu Pro Arg His Tyr Arg Ile 140 Ser Gin Ala Arg Val 220 Asn Thr Ala Gin Ser Ser le Gin Al a Leu Pro Leu 125 Arg Arg Asn Pro Gly 205 Ala Ile Glu Gln Ala 285 Gin Gin Val Ser Ala Phe Gly Gin 110 Ser Val Asp Phe Gly 190 Ala Val1 Ile Ala Tyr 270 Arg His Ala Glu Asn Glu Asp Gly Arg Glu Asp Ile 160 Thr Leu Ser Ala Met 240 Ala Asp Asp Ala Gly Val Ser Thr Leu Gin Met Pro Ser Asp Thr Pro WO 01/00843 290 295 Giu Thr Pro Giu His Ser Asn Thr Gin Pro 305 310 <210> 347 <211> 1662 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS <222> (101)..(1639) <223> RXA01508 <400> 347 ttccgggacg tttccgtgat ctcgtggaag atcccaaact PCTIIBOO/00923 300 ccgttccggt gccgtcgtgg ccgccgtcat cttgatcgtg gtgggaaccg taaacgctgc atg tct gat tta Met Ser Asp Leu ccc atc tgg Pro Ile Trp tcg ggg ctg Ser Gly Leu aac ggt ggc Asn Gly Gly gct gcc ctt Ala Ala Leu cct gcg caa Pro Ala Gin ggt tgt tcc Gly Cys Ser tcc ctg tgg Ser Leu Trp ggc gct gaa Gly Ala Giu 120 gcc gac gcc Ala Asp Ala 135 gat tac ctt Asp Tyr Leu tgg Trp tat Tyr att Ile ctt Leu t tc Phe ctg Leu ctg Leu c ca Pro acc Thr gca ctg Leu gag Glu gta Val1 ggt Gly ctc Leu 75 gtg Val1 gtg Val1 ctg Leu aca Thr ctt t ta Leu gcc Ala acc Thr 45 ctg Leu gtg Val1 tat Tyr gcc Ala atg Met 125 tct Ser ggt Gly tcc gtc Ser Val gta tcg Val Ser ctc atc Leu Ile gtc aag Val Lys acc ctc Thr Leu acc ttc Thr Phe gct gca Ala Ala atg atc Met Ilie gtt gcc Val Ala ctg gcc Leu Ala att Ile tcc Ser gca Ala ttt Phe gga G ly acc Thr ggc Giy caa Gin 130 ttg Leu cc t Pro tgt Cys acc Thr ggt Gly ctc Leu ctt Leu atc Ile atc Ile 115 ggc Gly aat Asn ttt Phe Ala Leu Leu WO 01/00843 150 ctg ccg tgg Leu Pro Trp aac ctc gtt Asn Leu Val ctt ccg cgc Leu Pro Arg 200 atc gca gcg Ile Ala Ala 215 acc gcc cgc Thr Ala Arg 230 tct gac tac Ser Asp Tyr ctc tac ctc Leu Tyr Leu tat aca gaa Tyr Thr Giu 280 gtg tta atc 1027 Val Leu Ile 295 cga ttc cca 1075 Arg Phe Pro 310 atc gaa gta 1123 Ile Giu Val gaa gat ccc 1171 Giu Asp Pro cgc tcc ggc 1219 Arg Ser Gly 360 ctt ccc gac 1267 ggc Gly 170 gca Ala cac His gcc Ala cag Gin gac Asp 250 ggc Gly ctg Leu ggc Gly atg Met aac Asn 330 gtc Val1 aat Asn atg Met t tc Phe ttc Phe gtg Val1 220 tac Tyr gta Val ttg Leu tat Tyr ggc Gly 300 atc Ile gtg Val atc Ile ggc Gly cgc ggt Arg Gly gtg ggc Val Gly 190 gta tcc Val Ser 205 ttg gtg Leu Val cgc gac Arg Asp gtg aca Val Thr cag tat Gin Tyr 270 cca agc Pro Ser 285 gat ggc Asp Gly acc caa Thr Gin ctg cgc Leu Arg atc gtt Ile Val 350 gaa act Giu Thr 365 acc atg 160 gca gca Aia Ala gtg ctg Vai Leu gtg gcg Val Ala tcc gac Ser Asp 225 gtg atc Val Ile 240 cga ggc Arg Gly act cgt Thr Arg aat cca Asn Pro gca gca Ala Aia 305 gaa tta Giu Leu 320 gac aat Asp Asn gac gct Asp Ala gat tcc Asp -Ser agg ctg gga Gly cga Arg 195 ctt Leu atc Ile tca Ser gac Asp cag Gin 275 gca Ala gaa Giu cca Pro gga Gly acc Thr 355 atc Ile tca PCTIIBOOIOO923 165 atg atc 643 Met Ile 180 cat ttg 691 His Leu ctc gcg 739 Leu Ala gtt gcc 787 Val Ala cac caa 835 His Gin 245 cga cgc 883 Arg Arg 260 cat aga 931 His Arg gaa tcg 979 Giu Ser ctc ctc Leu Leu gaa gtc Giu Val 325 gcg atg Ala Met 340 tgg ctg Trp Leu atc gat Ile Asp gaa gag cca aac aac gac WO 01/00843 WO 0100843PCT/IBOO/00923 Leu Pro 375 ttc tac 1315 Phe Tyr 390 gtg caa 1363 Val Gin gga gca 1411 Gly Ala gtg cat 1459 Val His Asp Pro Asn Asn Asp Thr met Ala Arg Leu Tyr Ser Giu Giu acc ttg gcc cga Thr Leu Ala Arg 395 tcc tcc agc gcc Ser Ser Ser Ala 410 acc ttg aaa tcg Thr Leu Lys Ser 380 gca cga ctg aac gaa Ala Arg Leu Asn Giu 400 tac acc act cca gat Tyr Thr Thr Pro ASP 415 385 caa ggc cgc atg gtg Gin Gly Arg Met Val 405 gtg ttc tgg cga gtt Val Phe Trp Arg Val 420 gcg ggc tgt Ala Gly Cys 425 gtt ccc Val Pro 430 aca ttt ggc gac tgg Thr Phe Gly Asp Trp 445 tta gag ctt cgg gaa Leu Glu Leu Arg Giu gaa caa gtc atc cca tat cac Giu Gin Val Ile Pro Tyr His 435 ggc ttc caa ctg tgt ggc cct Gly Phe Gin Leu Cys Giy Pro 450 gac acc ccg cca ctg act ttc Asp Thr Pro Pro Leu Thr Phe gcc gac atg 1507 Ala Asp Met 455 ctt aat gat 1555 Leu Asn Asp 470 cag cct cgt 1603 Gin Pro Arg gaa Glu 460 gaa gtt ctg gtg Giu Val Leu Val 475 465 gct gct ggg gtg Ala Ala Gly Val 480 ttt Phe gag gat 1649 Giu Asp ctg Leu gaa Glu cgc Arg 505 ttg gaa cct tcc acg ctg gat cat Leu Glu Pro Ser Thr Leu Asp His 490 495 aag gga tac cgc gaa tca ggc gac Lys Gly Tyr Arg Giu Ser Gly Asp 510 ggg ttg gat aat Gly Leu Asp Asn 485 ccc cgc gtg gtg Pro Arg Val Val 500 tagc tgcaac gatgcgctgt gtg 1662 <210> 348 <211> 513 <212> PRT <213> Corynebacterium giutarnicum <400> 348 Met Ser Asp Leu Gly Pro Ile Trp Arg Trp Leu Leu Leu Val Ser Val 1 5 10 Ser Ilie Cys Ala Ala Ser Gly Leu Val Tyr Giu Leu Ala Leu Val Ser 25 Leu Ser Thr Ser Leu Asn Gly Gly Gly Ilie Val Glu Thr Ser Leu Ile 40 WO 01/00843 WO 0100843PCT/EBOOIOO923 Val Pro Leu Ala Ile Gin Thr 145 Trp Ala Leu Ala

ASP

225 Ile Gly Arg Pro Ala 305 Leu Asn Ala Gly Leu Leu Ile Ile 115 Gly Asn Phe Gly Arg 195 Leu Ile Ser Asp Gin 275 Ala Glu Pro Gly Thr 355 Tyr Asn Ile Gly 100 Leu Arg Al a Val Met 180 His Leu Val Hius Arg 260 His Glu Leu Glu Ala 340 Trp Val Trp Gly Gin Val Leu Ala Leu 165 Ile Leu Ala Ala Gin' 245 Arg Arg Ser Leu Val1 325 Met Leu Ala Pro 70 Gly Ser Gly Ala Asp 150 Leu Asn Leu Ile Thr 230 Ser Leu Tyr Val1 Arg 310 Ile Glu Arg Ala Ala Cys Leu Ala

ASP

135 Tyr Pro Leu Pro Ala 215 Ala Asp Tyr Thr Leu 295 Phe Glu Asp Ser Leu Gin Ser Trp Glu 120 Ala Leu Trp Val Arg 200 Ala Arg Tyr Leu Glu 280 Ile Pro Val1 Pro Gly 360 Gly Thr Ala Ile 105 Leu Lys G ly Leu Ala 185 Thr Leu Ala Gin Asn 265 Ser Ile Ser Ala Arg 345 Gly Leu Phe Leu 90 Leu Pro Thr Ala Gly 170 Ala His Ala Gin Asp 250 Gly Leu Gly Met Asn 330 Val1 Asn Ala Leu Gly Val Leu Tyr Ile Ala Leu Met 125 Gly Ser 140 Leu Gly Met Arg Phe Val Phe Val 205 Val Leu 220 Tyr Arg Val Val Leu Gin Tyr Pro 285 Gly Asp 300 Ile Thr Val Leu Ile Ile Gly Glu 365 Leu Giu Phe Thr 110 Thr Leu Gly Gly Gly 190 Ser Val Asp Thr Tyr 270 Ser G ly Gin Arg Val 350 Thr Val Thr Thr Ala Met Val Leu Ala 175 Cys Val1 Lys Pro Giu 255 Ser Leu Leu Val Ser 335 Asp Tyr

LYS

Leu Phe Ala Ile Ala Ala 160 Ala Val1 Val Ser Val1 240 Arg Thr Asn Ala Glu 320 Asp Asp Asp WO 01/00843 Ser Ile Ii 370 Leu Tyr Se 385 Gin Gly Ax Val Phe Tr Val Ile Pr 43 Gin Leu C) 450 Pro Pro LE 465 Phe Giy LE His Pro Ar Asp Asp Giu Vali 405 Vali His Pro Phe Asn 485 Val Leu Phe 390 Val1 Giy Val Aia Leu 470 Gin Giu Asn Arg 395 Ala Ser Phe Giu Leu 475 Giu Gly PCTIBOO/00923 Thr Met Aia Arg Arg Leu Asn Giu 400 Thr Thr Pro Asp 415 Gly Cys Giu Gin 430 Asp Trp Giy Phe 445 Arg Giu Asp Thr Aia Ala Giy Val 480 Ser Thr Leu Asp 495 Arg Giu Ser Gly 510 <210> 349 <211> 924 <212> DNA <213> Corynebacterium giutanicun <22 0> <221> CDS <222> (i0i)..(901) <223> RXA01757 <400> 349 cgcttattga acggatgcct ctcgatcaag ccaacgaggc gtaaaccacg tttccgtatt gtcttggagc cgaattcata tattgcacgt atttcagctg atg cca aca gca agc 115 Met Pro Thr Ala Ser cca att tat gat gtc gtt gtc gtc Pro Ilie Tyr Asp Val Vai Val Val gcc acg caa ctg ttg gac cgc gca Ala Thr Gin Leu Leu Asp Arg Ala gcc tgc tca aga gtt ggc ggc cga Ala Cys Ser Arg Val Gly Gly Arg 45 ttg ttc ctg gac ctc ggc gca aca gga Gly ggt Gly 30 gca Ala tgg 521 gcc ggc Ala Giy 15 cta aac Leu Asn gtg tct Val Ser ttc tgg att tct ggc ctc atc Ile Ser Gly Leu Ile atc aaa tgc ttc gaa Ile Lys Cys Phe Giu gtc caa cag tcc gat Vai Gin Gin Ser Asp ctc aac gaa cca ctt 163 211 259 307 WO 01/00843 WO 0100843PCTIIIBOO/00923 Leu Phe Leu Asp Leu Gly Ala Thr Trp, Phe Trp Leu Asn Giu Pro Leu 60 ctc Leu gat Asp aac Asn 105 ctt Leu ggg Gly aag Lys cca Pro gc t Ala 185 tgg Trp ggt Giy cat His ggc Giy gtc Val1 gcg Ala ccc Pro gcg Ala gac Asp tct1 Ser ccg Pro 170 gac Asp gca Ala ttt Phe tgg Trp, gca Ala 250 aat Asn ctt Leu ata Ile ctc Leu ccc Pro tcc Ser 155 gca Ala gtg Val aag Lys ggg Gly gca Ala 235 gtt Val1 aat ctc ggc ctc ggc aca ttc Asn Leu Giy Leu Gly Thr Phe ttt gag acg ctt gtc gac gcc Phe Giu Thr Leu Val Asp Ala 95 gac gct gct tca ggc agg ttc Asp Ala Ala Ser Gly Arg Phe 110 ggg ctt gca gcc cag ctc aag Gly Leu Ala Ala Gin Leu Lys 125 130 gtc cat tct ctc agt gag gaa Val His Ser Leu Ser Giu Glu 140 145 aaa cag att gtg agg gca aag Lys Gin Ile Val Arg Ala Lys 160 ctc gct gcc gag ttg att ggt Leu Ala Ala Glu Leu Ile Gly 175 cga aaa gca gcg cat cca caa Arg Lys Ala Ala His Pro Gin 190 gag aaa tac acc tta ccc aca Giu Lys Tyr Thr Leu Pro Thr 205 210 cat gag ctg ttc caa caa cca His Glu Leu Phe Gin Gln Pro 220 225 tca acg gaa gtt gcc act gag Ser Thr Glu Val Ala Thr Giu 240 cgt gca gga att cag gct gcg Arg Ala Gly Ile Gin Ala Ala 255 taaacctcgt attttccctg ata cct cag Pro Gin ccg agc Pro Ser 100 caa gca Gin Ala 115 cca gga Pro Gly gat ggg Asp. Gly cac gtc His. Vai ttc acc Phe Thr 180 cat ata His Ile 195 caa gcc Gin Ala ctc gga Leu Gly ttt ggt Phe Giy ctt caa Leu Gin gga ttt aat Gly Phe Asn cta aaa tct Leu Lys Ser 265 <210> 350 <211> 267 <212> PRT <213> Corynebacterium giutamicuni WO 01/00843 PCTIIBOO/00923 <400> 350 Met Pro Thr Ala Ser Pro Ile Tyr Asp Val Val Val Val Gly Ala Gly 1 5 10 Ilie Ser Gly Leu Ile Ala Thr Gin Leu Leu Asp Arg Ala Gly Leu Asn 25 Ile Lys Cys Phe Giu Ala Cys Ser Arg Val Gly Gly Arg Ala Val Ser 40 Val Gin Gin Ser Asp Leu Phe Leu Asp Leu Gly Ala Thr Trp Phe Trp 55 Leu Asn Giu Pro Leu Val Gin Gin Leu Vai Asn Asn Leu Gly Leu Gly 70 75 Thr Phe Pro Gin Aia Ilie Giu Gly Asp Ala Leu Phe Giu Thr Leu Val 90 Asp Ala Pro Ser Arg Leu Arg Giy Asn Pro Ilie Asp Ala Ala Ser Gly 100 105 110 Arg Phe Gin Ala Gly Ala Ser Ser Leu Ala Leu Gly Leu Ala Ala Gin 115 120 125 Leu Lys Pro Gly Val Leu Glu Leu Gly Asp Pro Val Hius Ser Leu Ser 130 135 140 Giu Giu Asp Gly Giu Ilie Vai Val Lys Ser Ser Lys Gin Ile Val Arg 145 150 155 160 Ala Lys His Val Ilie Ile Ala Vai Pro Pro Ala Leu Ala Ala Giu Leu 165 170 175 Ile Gly Phe Thr Leu Asp Leu Pro Ala Asp Val Arg Lys Ala Ala His 180 185 190 Pro Gin His Ilie Ala Val Met Asn Trp Ala Lys Giu Lys Tyr Thr Leu 195 200 205 Pro Thr Gin Ala Aia Ser Ala Gly Gly Phe Gly His Giu Leu Phe Gin 210 215 220 Gin Pro Leu Gly His Gly Arg Ilie His Trp Ala Ser Thr Giu Val Ala 225 230 235 240 Thr Giu Phe Gly Gly His Leu Giu Gly Ala Val Arg Ala Gly Ile Gin 245 250 255 Ala Ala Leu Gin Thr Gly Phe Asn Leu Lys Ser 260 265 <210> 351 <211> 636 <212> DNA <213> Corynebacteriun giutaxnicwn <220> <221> CDS <222> (101)..(613) WO 01/00843 WO 0100843PCTIIBOO/00923 <223> R.XA02159 <400> 351 tgatggacca gcgtccaaag ttttcgatga agcagaaaac actgctggtg tggctgctgg ccaaccagcc gaggtaagac cgcctccacg ctcagaaagc atg tcc ctt ggc tca 11! Met Ser Leu Gly Ser acc ccg tca aca Thr Pro Ser Thr ccg Pro gaa aac tta aat ccc gtg act cgc act Giu Asn Leu Asn Pro Val Thr Arg Thr 15 gca cgc Ala Arg caa gct ctc Gin Ala Leu ttg cag att ttg Leu Gin Ile Leu aaa caa aaa gtc Lys Gin Lys Val acc agc cag Thr Ser Gin atc acc cag Ile Thr Gin gta caa ctg tct gaa ttg ctg ctg gat gaa ggc atc Val Gin Leu Ser Giu Leu Leu Leu Asp Glu Gly Ile gcc acc Ala Thr ttg tcc cga gat Leu Ser Arg Asp gat gaa ctc ggt Asp Giu Leu Gly cgc aag gtt cgc Arg Lys Val Arg ccc Pro gat ggg gga cgc Asp Gly Gly Arg tac tac gcg gtc Tyr Tyr Ala Val cca gta gat agc Pro Val Asp Ser gcc cgc gaa gat Ala Arg Giu Asp c tc Leu cgg ggt ccg tcg Arg Giy Pro Ser aag ctg cgc cgc Lys Leu Arg Arg atg ctt Met Leu 100 gat gaa ctg Asp Giu Leu cgc acc ccg Arg Thr Pro 120 gtt tct aca gat Val Ser Thr Asp cat His 110 tcc ggc aac atc Ser Gly Asn Ile gcg atg ctg Ala Met Leu 115 ccg gga gct gcc Pro Gly Ala Ala cag Gin 125 tac ctg gca agt ttc atc gat agg Tyr Leu Ala Ser Phe Ile Asp Arg 130 gtg ggg Val Gly 135 ctg aaa gaa gtc Leu Lys Glu Val gtt Val 140 ggc acc atc gct Gly Thr Ile Ala gat gac acc gtt Asp Asp Thr Val ttc gtt ctc gcc cgt Phe Val Leu Ala Arg 150 gat Asp 155 ccg ctc-aca ggt Pro Leu Thr Gly aaa Lys 160 gaa cta ggt gaa Glu Leu Gly Glu tta Leu 165 ctc agc ggg cgc Leu Ser Gly Arg acc act Thr Thr 170 taaagcgccc ctagttcaag gct <210> 352 <211> 171 <212> PRT <213> Corynebacterium glutamicum <400> 352 Met Ser Leu Gly Ser Thr Pro Ser Thr Pro Glu Asn Leu Asn Pro Val WO 01/00843 Thr Arg Lys Val Ile Asp Ala Arg Pro Val Leu Arg Asn Ile Ser Phe 130 Giy -Asp 145 Giu Leu Arg Gin Gin Arg Ile Leu Leu Arg Vali Leu 165 Leu Leu Leu Giy Glu Leu Pro 120 Leu Leu Gly Ile Ser Ser Gly Asp Leu 105 Pro Lys Ala Arg Leu Glu Arg Arg Leu 90 Val Gly Giu Arg Thr 170 Ile Leu Leu Tyr Giy Thr Ala Val 140 Pro Leu Leu Asp Tyr Pro Asp Gin 125 Gly Leu PCTIIBOO/00923 Lys Gin Giu Gly Leu Gly Vai Gly Giu Lys Ser Giy Leu Ala Ile Ala Gly Lys 160 <210> 353 <211> 414 <212> DNA <213> Corynebacterium giutamicun <2 <221> CDS <222> (391) <223> RXN02154 <400> 353 cccagaacct cggcgaagtc agcgacaagc cagtcaaggt caccgttacc tcgcgaattc tcaccactgc aaccgcacct acc gca gaa cag gct cgc gca gta Thr Ala Glu Gin Ala Arg Ala Val acc ttc gtg cat gtt ctt cca gaa Thr Phe Val His Val Leu Pro Glu gtt ctt ggc tcc aac atg tgc cac Val Leu Gly Ser Asn Met Cys His 45 tat gaa gag Tyr Giu Giu 15 ggt gca cag Gly Ala Gin 30 gtg cag gta Val Gin Val gagcttcacc ccagtgcttg ttg aaa gaa ggc gtt 115 Leu Lys Giu Gly Val 1 ttc tat gca cag gaa 163 Phe Tyr Ala Gin Giu cca caa acc caa gca 211 Pro Gin Thr Gin Ala gaa att gat gag gaa 259 Glu Ile Asp Glu Giu WO 01/00843 WO 0100843PCT/IBOO/00923 gca ggc aaa gtc ctt gtt acc tcc gca atc gat aac ctc acc aag gga 307 Ala Giy Lys Val Leu Vai Thr Ser Ala Ile Asp ASn Leu Thr Lys Gly 60 act gcc ggc gcc gct gtt cag tgc atg aac tta agc gtt ggt ttt gat 355 Thr Ala Gly Ala Ala Val Gln Cys Met Asn Leu Ser Val'Giy Phe Asp 75 80 gag gca gca ggc ctg cca cag gtc ggc gtc gca cct taaagtagcg 401 Giu Ala Ala Gly Leu Pro Gin Vai Gly Val Ala Pro ccttaaagcg gcg 414 <210> 354 <211> 97 <212> PRT <213> Corynebacterium giutamicun <400> 354 Leu Lys Giu Gly Val Thr Ala Giu Gin Ala Arg Ala Val Tyr Giu Giu 1 5 10 Phe Tyr Ala Gin Giu Thr Phe Val His Val Leu Pro Giu Gly Ala Gin 25 Pro Gin Thr Gln Ala Val Leu Gly Ser Asn Met Cys His Val Gln Val 40 Glu Ile Asp Giu Giu Ala Gly Lys Val Leu Val Thr Ser Ala Ile Asp 55 Asn Leu Thr Lys Gly Thr Ala Giy Ala Ala Val Gin Cys Met Asn Leu 70 75 Ser Vai Gly Phe Asp Giu Ala Ala Gly Leu Pro Gin Vai Gly Val Ala 90 Pro <210> 355 <211> 1302 <2i2> DNA <213> Corynebacterixum giutanicun <220> <221> CDS <222> (i01)..(i279) <223> RXS00147 <400> 355 attgcatata atgcaatgaa ttgaataaac tacattcagg gttatcaacc agccaatttc ttttaaaaag gcagacacac gaaaggcgac aacagtcacc gtg agt aaa gac acc 115 Val Ser Lys Asp Thr 1 WO 01/00843 WO 0100843PCTfIIBOO/00923 acc acc tac cag Thr Thr Tyr Gin gtc acc gag atc Val Thr Giu Ile tcc gtt ccg gca Ser Val Pro Ala tac ctg Tyr Leu gtt ctt gca Val Leu Ala ggc acc acc Gly Thr Thr gga cgt acc ttc Gly Arg Thr Phe gga ttt ggc ttt Gly Phe Gly Phe gga gct atc Gly Ala Ile acc ggt tac Thr Gly Tyr ctt ggt gag gca Leu Gly Giu Ala ttc act acc gcc Phe Thr Thr Ala caa gaa Gin Giu acc atg acc gat Thr Met Thr Asp tcc tat cac cgc Ser Tyr His Arg cag Gin att gtt gtg gct Ile Val Val Ala gca cca cag atc Ala Pro Gin Ilie ggc G ly aac acc ggc tgg Asn Thr Gly Trp gat gag gac aac Asp Giu Asp Asn tcc cgc gac ggc Ser Arg Asp Gly att tgg gtt gca Ile Trp Val Ala ctt gtt atc cgc Leu Val Ile Arg gac ctc Asp Leu 100 gca gca cgt Ala Ala Arg atg gca ggc Met Ala Gly 120 gtg Val 105 tcc aac tgg cgc Ser Asn Trp Arg acc acc tcc ttg Thr Thr Ser Leu cag cag gaa Gin Gin Giu 115 acc cgc gca Thr Arg Ala cag ggc atc gtc Gin Gly Ile Val atc ggc gga atc Ile Gly Gly Ile gac Asp 130 ctg gtt Leu Val 135 cgc cac ctg cgc Arg His Leu Arg gaa ggt tcc att Giu Gly Ser Ile gcg ggc atc ttc Ala Gly Ile Phe ggc gct gac gca Giy Ala Asp Ala cag cgc cca gtt gaa gaa ctc gta gag atc gtc Gin Arg Pro Val Giu Glu Leu Val Glu Ile Val 155 *160 165 aag aat cag cca Lys Asn Gin Pro gca Ala 170 atg acc ggc gca Met Thr Gly Ala aa c Asn 175 ctc tcc gtt gag Leu Ser Val Giu gtc tct Val Ser 180 gct gat gaa Ala Asp Glu gtc gtg gcc Val Val Ala 200 tac gtc atc gaa Tyr Val Ile Giu gaa ggc gaa gag Giu Gly Giu Giu cgc cac acc Arg His Thr 195 cgt cgt ttc Arg Arg Phe tac gac ctg ggc Tyr Asp Leu Gly aag caa aac acc Lys Gin Asn Thr cca Pro 210 tct gca Ser Ala 215 cgc ggt gtt cgc Arg Gly Val Arg gtc atc gtg cct Val Ile Val Pro gaa acc cca ttc Giu Thr Pro Phe 787 835 gag Giu 230 gat atc aag cag Asp Ile Lys Gin tac Tyr 235 aac cca tca ggc Asn Pro Ser Gly gtg Val 240 ttc atc tcc aac Phe Ile Ser Asn cct ggc gat cct gca gca gca gac gtc atg gtt gat atc gtc cgc gaa WO 01/00843 Pro Gly Asp gtt ctt gaa Val Leu Giu att ctt ggc Ile Leu Giy 280 cac cgc ggc 1027 His Arg Gly 295 gac atc acc 1075 Asp Ile Thr 310 cag gaa ttc 1123 Gin Giu Phe ctc aac gac 1171 Leu Asn Asp tac tcc gtt i21i9 Tyr Ser Val 360 agc ccc ctg 1267 Ser Pro Leu 375 Pro gcc Ala 265 cgc Arg atc Ile gcc Ala gag Giu Gly 345 cag Gin ttt Phe Ala 250 gac Asp gca Ala aac Asn cag Gin ac c Thr 330 gtc Val1 tac Tyr gac Asp Ala Ala at~t cca Ile Pro ttc ggc Phe Giy gtt cca Val Pro 300 aac cac Asn His 315 gat ttc Asp Phe gtt gaa Val Giu cac cca His Pro cag ttt Vai Met 255 ttt ggc Phe Gly 270 gag acc Giu Thr aag aac Lys Asn ttc gca Phe Ala act gca Thr Ala 335 att gcg Ile Ala 350 gcc gct Ala Ala gag ctg Asp tgc Cys aag Lys atc Ile 305 aag Lys gtc Val aag Lys Gly gat Asp 385 Ile t tc Phe c tg Leu 290 acc Thr ggt Gly acc Thr tcc Ser dca Pro 370 gca Val1 Gly 275 aag Lys 9gc Gly gaa Giu cac His gga Gly 355 aat Asn gac PCTIIBOO/00923 Arg Glu 260 aac cag 931 Asn Gin ttc ggc 979 Phe Gly aag atc Lys Ile gca ggc Ala Giy 325 acc tgc Thr Cys 340 cgc gca Arg Ala gat gca Asp Ala gct cag Gin Phe Val Giu Leu Met Ala Asp Ala Gin aag aaa ggc gca taaataacat gccaaagcgt tca 1302 Lys Lys Gly Ala 390 <210> 356 <211> 393 <212> PRT <213> Corynebacterium giutamicum <400> 356 Val Ser Lys Asp Thr Thr Thr Tyr Gin Gly Val Thr Glu Ile Gly Ser 1 5 10 Val Pro Ala Tyr Leu Val Leu Ala Asp Gly Arg Thr Phe Thr Gly Phe 25 Gly Phe Gly Ala Ile Gly Thr Thr Leu Gly Giu Ala Val Phe Thr Thr 40 WO 01/00843 WO 0100843PCT/EBOO/00923 Ala Met Thr Gly Tyr Gin Giu Thr Met Thr Asp Pro Ser Tyr His Arg 55 Gin Ile Val Val Ala Thr Ala Pro Gin Ile Gly Asn Thr Gly Trp Asn 70 75 Asp Giu Asp Asn Glu Ser Arg Asp Gly Lys Ile Trp, Val Ala Gly Leu 90 Val Ile Arg Asp Leu Ala Ala Arg Val Ser Asn Trp Arg Ala Thr Thr 100 105 110 Ser Leu Gin Gin Glu Met Ala Gly Gin Gly Ile Val Gly Ile Gly Gly 115 120 125 Ile Asp Thr Arg Ala Leu Val Arg His Leu Arg Asn Giu Gly Ser Ile 130 135 140 Ala Ala Gly Ile Phe Ser Gly Ala Asp Ala Gin Arg Pro Val Giu Giu 145 150 155 160 Leu Val Giu Ile Val Lys Asn Gin Pro Ala Met Thr Gly Ala Asn Leu 165 170 175 Ser Val Glu Val Ser Ala Asp Giu Thr Tyr Val Ile Giu Ala Giu Gly 180 185 190 Giu Giu Arg His Thr Val Val Ala Tyr Asp Leu Gly Ile Lys Gin Asn 195 200 205 Thr Pro Arg Arg Phe Ser Ala Arg Gly Val Arg Thr Val Ile Val Pro 210 215 220 Ala Glu Thr Pro Phe GlU ASP Ile Lys Gin Tyr Asn Pro Ser Gly Val 225 230 235 240 Phe Ile Ser Asn Gly Pro Gly Asp Pro Ala Ala Ala Asp Val Met Val 245 250 255 Asp Ile Val Arg Giu Val Leu Giu Ala Asp Ile Pro Phe Phe Gly Ile 260 265 270 Cys Phe Gly Asn Gin Ile Leu Gly Arg Ala Phe Gly Met Giu Thr T'yr 275 280 285 Lys Leu Lys Phe Gly His Arg Gly Ile Asn Val Pro Val Lys Asn His 290 295 300 Ile Thr Gly Lys Ilie Asp Ile Thr Ala Gin Asn His Gly Phe Ala Leu 305 310 315 320 Lys Gly Giu Ala Gly Gin Giu Phe Giu Thr Asp Phe Gly Thr Ala Ile 325 330 335 Val Thr His Thr Cys Leu Asn Asp Gly Val Val Giu Gly Ile Ala Leu 340 345 350 Lys Ser Gly Arg Ala Tyr Ser Val Gin Tyr His Pro Giu Ala Ala Ala 355 360 365 WO 01/00843 PCTIIBOO/00923 Gly Pro Asn Asp Ala Ser Pro Leu Phe Asp Gin Phe Val Glu Leu Met 370 375 380 Asp Ala Asp Ala Gin Lys Lys Gly Ala 385 390 <210> 357 <211> 924 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (901) <223> RXS00905 <400> 357 cgctgcccct ctatgctgct cctagttacc cctgcacaaa tagcggtttt tctcacgcat tctgcatcga gtcgggtcga cgtatataag gtggaaaggc atg acc caa ttc Met Thr Gin Phe aac gcg Asn Ala ttt .acc Phe Thr cca aac Pro Asn aca ctg Thr Leu gga acc Gly Thr aag gta Lys Val gat ctc Asp Leu cac ggc His Gly ctg gtc Leu Val 135 caa Gin cag Gin cta Leu ac c Thr acc Thr c tg Leu tgg Trp ac c Thr 120 atg Met Ctt Leu aaa Lys gag Glu gca Ala t tc Phe tac Tyr acc Thr tgg Trp c tc Leu gag Giu att Ile tac Tyr ctC Leu 99c Gly cac His cct Pro ggc Gly gca Ala 140 aac Asn gtg Val1 gcc Ala acc Thr ac c Thr ttc Phe ttt Phe cgc Arg 125 ctg Leu atc Ile tC t Ser ggc Gly gtc Val cgc Arg gac Asp gaa Giu 110 ggc Gly cgc Arg gaa Glu 15 ttc Phe gcg Ala agc Ser aag Lys gtt Val 95 C tC Leu gcc Ala gc c Al a gag aac Asn aac Asn aaa Lys gaa Glu ggc Gly 80 gtc Val1 ac c Thr gc t Ala gtc Val1 ggc cag Gin agc S er aaa Lys gaa Glu gca Ala cct Pro 100 gc t Ala ggc Gly ggc Gly atg gaa Glu atc Ile ga t Asp gaa Giu gat Asp cca Pro ttg Leu 99c G ly aac Asn gac Asp gga acc aca ctc aac ctc acc tac gtg gtC WO 01/00843 Thr Thr LE 150 ggc gga gc Gly Gly A) gca gat gt Ala Asp Vi cca acc tt Pro Thr Lf 2( gtg gac ac Val Asp T1~ 215 gcc cca gi Ala Pro AE 230 gat gaa cz Asp Giu Hi ctg gaa g Leu Glu GJ Asn ctc Leu atc Ile 185 acc Thr ctt Leu gc t Ala gga Gly cga Arg 265 Leu agc Ser 170 ttg Leu act Thr gaa Glu gt t Val cgc Arg 250 gcc Ala Thr 155 gcg Ala att Ile acc Thr ggc Gly gc t Ala 235 ac c Thr tta Leu Tyr Val Val Glu Gly Ser Glu 160 ctc atc aag gac aag cct gag Leu Ile Lys Asp Lys Pro Glu 175 gca gac agc gga aac gct tcc Ala ASP Ser Gly Asn Ala Ser 190 ctg cgc ggt ggc gga cag gtc Leu Arg Gly Gly Gly Gin Val 205 210 gct gtt cac tcc ggc cag aac Ala Val His Ser Gly Gin Asn 220 225 gct ctc gtg cgc gtt ctg gat Ala Leu Val Arg Val Leu Asp 240 gtt atc gac ggc tgt caa cac* Val Ile Asp Gly Cys Gin His 255 tgatccagag actttccgca gcg PCTIBOO/00923 Met Gly 165 ttc gac 643 Phe Asp 180 ggc acc 691 Gly Thr gtc acc 739 Val Thr ggc gct 787 Gly Ala ttg cgc 835 Leu Arg 245 cgc aaa 883 Arg Lys 260 <210> 358 <211> 267 <212> PRT <213> Corynebacteriun giutamicun <400> 358 Met Thr Gin Phe Giu Asn Ala Gin I 1 5 Gin Arg Glu Gin Ilie Phe Thr Gin 1 Ser Val His Ser Asp Pro Asn Leu 1 Giu Trp Val Lys Glu Thr Leu Thr J Phe Ala Ala Glu Asp Gly Thr Thr 2 70 Ser Giu Gly Ala Pro Lys Val Leu 1 Pro Ser Gly Pro Leu Asp Leu TrpI 100 Glu Arg Asp Aia Giy His Gly Thr 115 120 Lys Glu Asp Gly Ile 75 Ser Asn Tyr Glu Phe Ala Ser Lys Vai Leu Ala Asn Asn Lys Giu Gly Vai Thr Ala WO 01/00843 WO 0100843PCTIJIBOO/00923 Asp Giu 145 Ser Pro Ala Gin Gin 225 Leu Gin Gly Gly Met Phe 180 Gly Val1 Gly Leu Arg 260 Leu Thr 150 Gly Ala Pro Val1 Ala 230 Asp Leu Val 135 Thr Gly Asp Thr Asp 215 Pro Giu Glu Leu Val1 Ile Asp Arg 205 Val Leu Ile Arg Val Lys Ser 190 Gly His Val1 Asp Ala Glu Asp 175 Gly Gly Ser Arg Gly 255 <210> 359 <211> 627 <212> DNA <213> Corynebacteriun giutamicun <22 0> <221> CDS <222> (101)..(604) <223> RXS00906 <400> 359 accgtggaca cccttgaagg cgctgttcac tccggccaga gctgttgctg ctctcgtgcg cgttctggat actttgcgcg gtc Val1 t tc Phe ggc Gly acc Thr acggtggcgc tgccccagat atg aac acg gac gca Met Asn Thr Asp Ala 1 tgg aag ggc gag cct Trp Lys Gly Glu Pro atc ctc gac ggt gta Ile Leu Asp Gly Val atg ctg tgg tcc agg Met Leu Trp Ser Arg cca gtg gca gaa gca Pro Val Ala Giu Ala acc Thr WO 01/00843 WO 0100843PCTIiBOO/00923 ctc aac gca gtg ccc gca acg gcg tcc gcc aag cta aac ctt cgc gtg 355 Leu Asn Ala Val Pro Ala Thr Ala Ser Ala Lys Leu Asn Leu Arg Val 75 80 cca gca ggc ctg gaa gca aac gat gtg gcc gag aag ctg aag cag cac 403 Pro Ala Gly Leu Giu Ala Asn Asp Val Ala Giu Lys Leu Lys Gin His 95 100 ctg atc aat cac aca cct tgg ggc gca aag atc acg gtg gag atc gat 451 Leu Ile Asn His Thr Pro Trp Gly Ala Lys Ile Thr Val Glu Ile Asp 105 110 115 gac att aac caa ccg ttc tcc acc gat att acc ggc cct gca atg tcc 499 Asp Ile Asn Gin Pro Phe Ser Thr Asp Ile Thr Gly Pro Ala Met Ser 120 125 130 acc ctg gcg tcc tgc ctg agc gct gcg tac gag ggc aag gat ctt gtc 547 Thr Leu Ala Ser Cys Leu Ser Ala Ala Tyr Giu Gly Lys Asp Leu Val 135 140 .145 acc gaa ggc agc ggc gga tcc att cca ctg tgt acc gaa ctg att gag 595 Thr Giu Gly Ser Gly Gly Ser Ile Pro Leu Cys Thr Glu Leu Ile Glu 150 155 160 165 gtc aac cca taagcagaat tggcactcta cgg 627 Val Asn Pro <210> 360 <211> 168 <212> PRT <213> Corynebacterium glutanicun <400> 360 Met Asn Thr Asp Ala Pro Leu Ser Thr Ala Val Asn Thr Thr Ala Asn 1 5 10 Trp Lys Gly Giu Pro Tyr Asp Pro Glu Thr Phe Arg Ser Asp Ala Gly 25 Ile Leu Asp Gly Val Asp Ile Met Gly Asp Gly Asp Asn Pro Ala Ser 40 Met Leu Trp Ser Arg Pro Ala Ile Ser Ile Thr Gly Phe Thr Ser Thr 55 Pro Val Ala GlU Ala Leu Asn Ala Val Pro Ala Thr Ala Ser Ala Lys 70 75 Leu Asn Leu Arg Val Pro Ala Gly Leu Glu Ala Asn Asp Val Ala Glu 90 Lys Leu Lys Gin His Leu Ile Asn His Thr Pro Trp, Gly Ala Lys Ile 100 105 110 Thr Val Glu Ilie Asp Asp Ile Asn Gln Pro Phe Ser Thr Asp Ile Thr 115 120 125 Gly Pro Ala Met Ser Thr Leu Ala Ser Cys Leu Ser Ala Ala Tyr Glu 533 WO 01/00843 WO 0100843PCTIiBOO/00923 Gly Lys Asp Leu Val Thr Glu Gly Ser Gly Gly Ser Ile Pro Leu Cys 145 150 155 160 Thr Glu Leu Ilie Glu Val Asn Pro 165 <210> 361 <211> 246 <212> DNA <213> Corynebacteriun glut anic ur <220> <221> <222> <223>

CDS

(101)..(223) RXS0 0907 <400> 361 cctgagcgct gcgtacgagg gcaaggatct tgtcaccgaa ggcagcggcg gatccattcc actgtgtacc gaactgattg aggtcaaccc ataagcagaa ttg gca ctc tac Leu Ala Leu Tyr 1 ggt Gly 115 gtg gaa gaa ccc Val Glu Glu Pro ccc aat gag att Pro Asn Glu Ile aac tac acc aag Asn Tyr Thr Lys ctc acc gtt atc cac tcc gct aat gaa Leu Thr Val Ile His Ser Ala Asn Glu 15 tct gtt gac Ser Val Asp cgc gat atc gcc acc gca gaa Arg Asp Ile Ala Thr Ala Glu 30 tagacccaaa agcaggcgtt aac gca ttg ttc ctg ctc Ala Leu Phe Leu Leu <210> 362 <211> 41 <212> PRT <213> Corynebacteriun glutanicun <400> 362 Leu Ala Leu Tyr Gly Val Glu Glu Pro Leu Thr Val Ile His Ser Ala 1 5 10 Asn Glu Ser Ala Leu Phe Val Asp Pro Asn Glu Ile 25 Leu Leu Asn Tyr Thr Lys Arg Asp Ile Ala Thr Ala Glu <210> 363 <211> 1281 <212> DNA <213> Corynebacteriun glutanicurn <22 0> WO 01/00843 <221> CDS <222> (101)..(1258) <223> RXS02001 <400> 363 gcggtttcgt catggataag gactgtgttc gggaccattg PCTIiBOO/00923 cgatactcgt gtcaaaaggc gatagtccag catagaccgt gctttatcga aggtgaaccc atg ccc gtt Met Pro Val atc aat Ile Asn agt Ser cat His gtg Val atc Ile ccc Pro att Ile t gc Cys ctg Leu cct Pro gtc Val 150 ccc Pro gcc Ala ggt Gly atc Ile caa Gin gcc Ala gcg Aia gtc Val acc Thr ggc Giy gcc Ala gcg Ala 135 c tc Leu ggc G ly gct Ala gcc Ala gcc Al a aac Asn gag Glu aaa Lys gtc Val1 ggC Gly cac His gag Glu 120 gaa Giu gac Asp ctg Leu gtc Val aaa Lys agt Ser ccc Pro cag Gin ac c Thr 9gc Gly gtc Val gac Asp 105 acg Thr gaa Giu cgc Arg ccg Pro gac Asp 185 ccg Pro ttt Phe gaa Glu ctg Leu ggt Gly ctg Leu gac

ASP

ggc Gly cgc Arg aac Asn ttt Phe ctt Leu 170 acg Thr cac His tcc Ser atc Ile cgc Arg atc Ile cgc Arg tat Tyr c ac His aat Asn ggc Gly gcc Ala 155 ggc Gly ttc Phe caa Gin gag Glu ttt Phe ttc Phe 45 gcc Ala gat Asp tc g Ser acc Thr gc a Ala 125 ggc Gly gcc Ala ttt Phe atc Ile cgc Arg acc Thr tgt Cys gtg Val att Ile gac Asp ac c Thr c tg Leu ac c Thr ggC Gly gtc Val acg Thr 175 att Ile ccc Pro cgg Arg act Thr att Ile cgc Arg.

ccg Pro aaa Lys gcc Ala ctg Leu 130 gtc Val ctg Leu 9gc Gly cgc Arg gca Al a cgt Arg gcg Ala c ac His gag Giu ctg Leu atg Met gcc Ala 115 atc Ile gat Asp cac His ccg Pro ggc Gly 195 gcc Ala WO 01/00843 WO 0100843PCTIIBOO/00923 200 205 210 att gtc caa gcg ttt caa acg ata gtc agc cgg aat cac aat ccg gtc 787 Ile Val Gin Ala Phe Gin Thr Ile Vai Ser Arg Asn His Asn Pro Val 215 220 225 gag gac ctt gtc gtg tcg gtc acg caa atc cac acc ggc agc gcc gat 835 Glu Asp Leu Val Val Ser Val Thr Gin Ile His Thr Gly Ser Ala Asp 230 235 240 245 aat atc atc ccc gaa acc gcc tat atc aac ggc act gtc cgc acc ttc 883 Asn Ilie Ilie Pro Giu Thr Ala Tyr Ile Asn Gly Thr Val Arg Thr Phe 250 255 260 aac aaa gac gtg cag gcc atg gtc atc acg cgg atg gaa gaa atc gtc 931 Asn Lys Asp Val Gin Ala Met Vai Ile Thr Arg Met Glu Glu Ile Vai 265 270 275 gcg ggc caa gct gca gcc tat ggg gtc gag gcg acg ctg acc tac aac 979 Ala Gly Gin Ala Ala Aia Tyr Gly Val Giu Ala Thr Leu Thr Tyr Asn 280 285 290 cgc aac tat ccc gcc acc att aac gac gcc gcc aaa gcc gcc atc gct 1027 Arg Asn Tyr Pro Ala Thr Ilie Asn Asp Ala Ala Lys Ala Ala Ile Ala 295 300 305 gcc gaa gtc gcg ggc gag gtc ggc ctc ggg gtc aac ccg aac ggc tcg 1075 Ala Glu Val Ala Gly Giu Val Gly Leu Gly Val Asn Pro Asn Gly Ser 310 315 320 325 cgc ggg atg ggg gcc gag gat ttc tcg tat ttc ctc gaa aag cgc ccg 1123 Arg Gly Met Gly Ala Giu Asp Phe Ser Tyr Phe Leu Glu Lys Arg Pro 330 335 340 ggt gcc tac ctg ttc gtc ggt aat ggc gac agc gcg ggc ctt cac aac 1171 Gly Ala Tyr Leu Phe Val Gly Asn Gly Asp Ser Ala Gly Leu His Asn 345 350 355 ccc gcc tat aat ttc aac gac gag gcc gcg ccc tac ggc gca tcg ttc 1219 Pro Ala Tyr Asn Phe Asn Asp Giu Ala Ala Pro Tyr Gly Ala Ser Phe 360 365 370 ttg gcc cgc atg gca gaa cgc ccc ttg ccg tta aag ggc tgatccatgg 1268 Leu Ala Arg Met Ala Glu Arg Pro Leu Pro Leu Lys Gly 375 380 385 cgctcgaaga tgc 1281 <210> 364 <211> 386 <212> PRT <213> Corynebacteriumf giutamicumr WO 01/00843 <400> 364 Met Pro Val 1 Trp Arg Arg Glu Thr Ala Gu Ile Hius Gly Arg Giu Leu Pro Leu Gly Lys Met Gly Ala Ala 115 Ala Leu Ile 130 Met Val Asp 145 Ala Leu His Ala Gly Pro Gly Arg Gly 195 Val Ala Ala 210 Asn His Asn 225 Thr Gly Ser Thr Vai Arg Met Glu Giu 275 Thr Leu Thr 290 Lys Ala Ala 305 PCTIiBOO/00923 Ile His Al a Thr Ala Thr His 100 Lys Phe Giu Asn Ile 180 Gly Val1 Pro Ala Thr 260 Ile Tyr Ile Ser His Val Ile Pro 70 Ile Cys Leu Pro Val1 150 Pro Ala Gly Ile Glu 230 Asn Asn Ala Arg Al a 310 Ile Gin Al a Ala 55 Val Thr Gly Al a Ala 135 Leu Gly Ala Al a Val 215 Asp Ile Lys Gly Asn 295 Glu Ala Asn Giu Lys Val Gly His Glu 120 Glu Asp Leu Val Lys 200 Gin Leu Ile Asp Gin 280 Tyr Val1 Ser Pro 25 Gin Thr Gly Val Asp 105 Thr Glu Arg Pro Asp 185 Pro Ala Val1 Pro Val1 265 Ala Pro Ala Phe 10 Giu Leu Gly Leu Asp Gly Arg Asn Phe Leu 170 Thr His Phe Val Glu 250 Gin Ala Ala Gly Ser Ile Arg Ile Arg 75 Tyr His Asn Gly Ala 155 Gly Phe Gin Gin Ser 235 Thr Ala Ala Thr Giu 315 Asp Ser Ser Ile Ala Ala Thr Phe Gly 140 Ile His Asp Thr Thr 220 Val1 Ala Met Tyr Ile 300 Val Met Asp Gly Leu Met Thr Met 110 Gly Ala Giu Met Asn 190 Asp Val1 Gin Ile Ile 270 Val1 Asp Leu Thr Cys Val Ile Asp Thr Leu Thr Gly Val Thr 175 Ile Pro Ser Ile Asn 255 Thr Glu Ala G ly Arg Val

ASP

His Ala Pro Leu Val Val Tyr 160 Thr Thr Ile Arg His 240 Gly Arg Ala Ala Val 320 WO 01/00843 WO 0100843PCTIBOO/00923 Asn Pro Asn Gly Ser 325 Arg Gly Met Gly Glu Asp Phe Ser Tyr Phe 335 Leu Glu Lys Ala Gly Leu 355 Tyr Gly Ala 370 Arg 340 Pro Gly Ala Tyr Phe Val Gly Asn Gly Asp Ser 350 Ala Ala Pro His Asn Pro Ala Asn Phe Asn Asp Ser Phe Leu Arg Met Ala Glu Arg 380 Pro Leu Pro Leu Lys Gly 385 <210> <211> <212> <213> <220> <221> <222> <223> 365 1386

DNA

Corynebacterium

CDS

(1363) RXS02101 glut amicum <400> 365 gccatggaat gctccgttga acgcaacagc cttaaataca atcccctcct ataagccaag agttttagtg tcgctgcgca ggtactctac tatctaatcc atg agc cgc att Met Ser Arg Ile 1 gaa ctt cta aac Glu Leu Leu Asn cat ggt gtt gat His Gly Val Asp tcg tgg caa gag Ser Trp Gin Giu gcc gca Ala Ala tat cag gat Tyr Gin Asp acc gca gat Thr Ala Asp cac gaa cat cct gag ctc tcc ggc ttc His Glu His Pro Glu Leu Ser Gly Phe gaa tca gag Glu Ser Giu tgt gag gtg Cys Glu Val cgc att cag aaa Arg Ile Gin Lys ctc gag cgt ttt Leu Giu Arg Phe 259 att cca Ile Pro aat gtt ggc ggt Asn Val Gly Gly ggc att ctg gcc Gly Ile Leu Ala ttc cga aat ggg Phe Arg Asn Giy aca gat cct ggt Thr Asp Pro Gly gcc Al a cct gtt gcg tta Pro Val Ala Leu cgc gca gat ttc Arg Ala Asp Phe ggc ctt ccc gtc Gly Leu Pro Val aag Lys gaa atc acc gga gtt ccg ttt gct tcc Giu Ile Thr Gly Val Pro Phe Ala Ser 95 act cgt Thr Arg 100 atg cgt ccg Met Arg Pro gat ggg gca aat gtc cat gtc atg cac Asp Gly Ala Asn Val His Val Met His 110 gca tgc ggc Ala Cys Gly 115 451 WO 01/00843 cac gat His Asp gag cgt Giu Arg 135 tcg gag Ser Giu 150 gtt gat Val Asp ccc ggt Pro Gly gcc tgc Ala Cys tcc atg Ser Met 215 gtC gtg Vai Val 230 ttc gcc Phe Ala acc att Thr Ile gac aaa Asp Lys ggt gaa 1027 Gly Giu.

295 ttc gcc 1075 Phe Ala 310 gtg cgc 1123 Val Arg tac cgg 1171 gtC Val1 120 cgc Arg gaa Giu ctg Leu gc t Ala gat Asp 200 cc t Pro cga Arg gtt Val1 cca Pro gtc Val1 280 tgC Cys cac His cct Pro tgg cac gtc acc gcg ctg ctt ggt gcg tgt gcc att His Val Thr Ala Leu Leu Gly Ala Cys Ala Ile 125 130 tgg Trp caa Gin 155 cgc Arg ac c Thr gaa Glu tcc Ser gga Gly 235 gtg Val gc t Ala aag Lys tcc Ser c tc Leu 315 gac Asp tcg ttc atc PheIle aag atg Lys Met 160 tgc ttt Cys Phe 175 atg cct Met Pro cag ggt Gin Gly act tat Thr Tyr cgc gag Arg Glu 240 cag tcg Gin Ser 255 ttg aac Leu Asn gcc atc Ala Ile gaa gaa Glu Giu acc cct Thr Pro 320 ggc gag Gly Glu 335 ccc tcc gcg Ala 145 gtC Val1 99c Gly ggc Gly cgc Arg gtt Val 225 gtt Val1 9gc Gly tgc Cys gaa Glu cCt Pro 305 gtt Val gat Asp att t tg Leu gcc Ala cag Gin ggt Gly agc Ser 210 gca Ala tct Ser aac Asn cgt Arg cgt Arg 290 gtc Val1 gtc Val1 tct Ser CC t PCTJIBOO/00923 tta gat 499 Leu Asp cag cca 547 Gin Pro ggt tta 595 Gly Leu 165 gta gtc 643 Val Val 180 ctc gct 691 Leu Ala cat ggt 739 His Gly atg att 787 Met Ile gag gat 835 Glu Asp 245 aac aac 883 Asn Asn 260 tac aac 931 Tyr Asn gtc cgt 979 Val Arg gag tac Glu Tyr gat act Asp Thr 325 gac gct Asp Ala 340 gca ttc WO 01/00843 Tyr Arg Trp Thr Ala Ser Giu Asp Phe 345 350 aac agc cct tac ctg tac tgg acg att 1219 Asn Ser Pro Tyr Leu Tyr Trp Thr Ile 360 365 tgg aca gaa gcc gta gaa aga gac cgc 1267 Trp Thr Glu Ala Val Glu Arg Asp Arg 375 380 aat cac atg gga gat ttc ctc cct gat 1315 Asn H-is Met Gly Asp Phe Leu Pro Asp 390 395 gcc acc cgc gca gcc gca gcc gcg ctg 1363 Ala Thr Arg Ala Ala Ala Ala Ala Leu 410 taatcatcta gttttctgcg acg 1386 <210> 366 <211> 421 <212> PRT <213> Corynebacterium giutamicum <400> 366 Met Ser Arg Ile Ser Glu Leu Leu Asn Pro ggt Gly gtg Val1 tat Tyr ctg Leu 415 Ile Pro Lys 355 acg ccg cgc Thr Pro Arg 370 tcg gat gtg Ser Asp Val 385 ccg acg atg Pro Thr Met tac ttg gga Tyr Leu Gly PCTLBOO/00923 Ala Phe gat cag Asp Gin cca gcc Pro Ala tcc gct Ser Ala 405 act aac Thr Asn 420 1 Trp Gly Phe Val Arg Phe Met Cys Ala Gin Phe Asp Phe Ala Ala His Ala 130 Leu Tyr Thr Ile Ser 70 Gly Met His Glu Ser Asp Asp Asn Asp Pro Pro Val 120 Arg Giu Phe Arg Val1 Pro Val His 105 His Asp Asn Asn His Ile Gly Gly Lys Asp Val Al a Ser His Giu Gin Gly Al a 75 Giu Gly Thr Trp Gin Val Pro Tyr Gly Val1 Thr Asn Leu 125 Gly Ala Asp Glu Leu Ile Ala Gly Val1 110 Leu Thr Asn Ser Ser Arg Ala Met Pro Val Ala Ile Met WO 01/00843 145 Val Ala Gly Gly Gin His Gly Gly Ala 195 Arg Ser Ala 210 Val Ala Ala 225 Val Ser Pro Gly Asn Thr Cys Arg Phe 275 Glu Arg Val 290 Pro Val Ilie 305 Val Val Phe Asp Ser Ilie Ile Pro Lys 355 Thr Pro Arg 370 Ser Asp Val 385 Pro Thr Met Tyr Leu Giy 150 Val1 Pro Ala Ser Val1 230 Phe Thr Asp Gly Phe 310 Vai Tyr Asn Trp Asn 390 Ala Asp Met Arg 205 Asp Val Thr Leu Tyr 285 Ile Asn Val Asp Thr 365 Asp Pro Val S er 190 Ile Pro Gly Leu Val1 270 Arg Glu Asn Phe Phe 350 Ile Arg Asp PCT/1B00100923 160 ys Phe et Pro in Gly hr Tyr rg Glu 240 in Ser eu Asn la Ile iu Glu hr Pro 320 iy Giu ro Ser ly Val al Ala yr Ala 400 Thr Arg Ala Ala Ala Ala Ala Leu Leu Thr 410 415 Thr Asn 420 <210> 367 <21i> 3462 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS WO 01/00843 <222> (101)..(3439) <223> RXS02234 <400> 367 acccagaggc cgctgccggc ccaaatgatg caagccccct PCT11B00100923 gtttgaccag tttgttgagc tgatggatgc agacgctcag aagaaaggcg cataaataac atg cca aag cgt tca Met Pro Lys Arg Ser ga t Asp cag Gin aag Lys atc Ilie gag Glu cac His aac Asn gtt Val cgc Arg cgt Arg 150 gaa Giu ctg Leu ggt Gly att Ile gca Ala gaa Giu atg Met ccg Pro cca Pro gca Ala gaa Giu cag Gin 135 tcc Ser c tc Leu ggc Gly ggc Gly aac Asri tgt Cys gag Giu ac c Thr gaa Giu atc Ilie gc t Al a ctc Leu 120 aag Lys cgc Arg ggc Gly tcc Ser gga Gly 200 cac His gaa Giu gga Gly gac Asp t ac Tyr gac Asp atc Ile 105 atc Ile ttc Phe gtc Val ctt Leu ggt Gly 185 ctt Leu c tc Leu gac Asp cgc Arg gaa Giu gac Asp 75 gtc Val1 ctg Leu gca Ala gat Asp c ac His 155 gta Val gca Ala gca Ala gtc Val1 tac Tyr gt c Val atg Met 60 aag Lys ctg Leu gat Asp gac Asp at t Ile 140 aac Asn gtc Val tac Tyr tc t Ser atc Ile t cc Ser acc Thr 45 gct Al a att Ile gca Ala cgc Arg atc Ilie 125 gtc Val1 atg Met gtg Val1 aac Asn cc t Pro 205 tcc Ser acc Thr atc Ile cac His gc t Ala ctt Leu ggc Gly gcc Ala acc Thr gaa Giu cca Pro 175 gaa Giu gca Ala at t Ile 20 gtg Val gca Ala c ca Pro cag Gin gca Ala 100 t ac Tyr gaa Glu tcc S er gtc Val1 ggt Gly 180 atc Ile gaa Glu ggC Gly ctg Leu acg Thr atc Ile Gly ctt Leu ggc Gly gat Asp gcg Al a gca Ala 165 ggc Gly gc t Ala gaa Glu 163 211 259 307 355 403 451 499 547 595 643 691 739 WO 01/00843 WO 0100843PCT/EBOO/00923 tcc atc Ser Ile 215 gca gac Ala Asp 230 ctt ggt tgg Leu Gly Trp aac gtt gtg Asn Val Val aag gaa Lys Giu 220 gtt atc Val Ilie 235 ttc gag ctc gag Phe Giu Leu Giu atg cgc gat acc Met Arg Asp Thr tgc tcc att Cys Ser Ile gtc gac gca Val Asp Ala c tg Leu 245 c tg Leu ggc gtg cac acc Gly Val His Thr ggc Gly 250 t tc Phe gac tct gtc acc Asp Ser Val Thr gtg Val 255 gat Asp cct gcc ctg Pro Ala Leu acc Thr 260 act gac cgt Thr Asp Arg cgc gag gtc Arg Glu Val 280 gaa Giu 265 ggc Gly cag aag atg Gin Lys Met cag ggt atc Gin Giy Ile gcc atc atc Ala Ile Ile 275 gtg gac acc Val Asp Thr tgt aac atc Cys Asn Ile cag ttc gcc atc Gin Phe Ala Ile 290 aac cca cgt gtg Asn Pro Arg Val aac cca 1027 Asn Pro 295 tct cgt 1075 Ser Arg 310 aag atg 1123 Lys met aac gac 1171 Asn Asp tac gtc 1219 Tyr Vai gct gat 1267 Ala Asp 375 tcc ctg 1315 Ser Leu 390 ctg gaa 1363 Leu Giu gtt gat ggc cgc atc Val Asp Gly Arg Ile 300 tcc tcc gct ctg gca Ser Ser Ala Leu Ala 315 gct gcc aag ctg gct Ala Ala Lys Leu Ala atc acc att gag atg Ile Thr Ile Giu Met 305 tcc aag gca acg ggc Ser Lys Ala Thr Gly 320 atc gga tac acc ctg Ile Gly Tyr Thr Leu 335 cca gct gcg ttt gag Pro Ala Ala Phe Giu ttc cca att gcc Phe Pro Ile Ala 325 gat gag atc acc Asp Giu Ile Thr 340 ccc acc atc gac Pro Thr Ile Asp 355 atc act Ilie Thr 345 gtg gtc Val Val 330 gg t Gly gaa acc Giu Thr 350 aag gcc cca cgc Lys Ala Pro Arg 365 ttg acc acc acc Leu Thr Thr Thr ttt gct ttc gag aag ttt gtc ggc Phe Ala Phe Giu Lys Phe Val Gly 370 atg aag tcc gtc ggt gag gtc atg Met Lys Ser Val Gly Giu Val Met act Thr 380 ggc cgc aac tac Gly Arg Asn Tyr 395 acc aag cag cag Thr Lys Gin Gin 410 att gca gca ctg aac Ile Ala Ala Leu Aen 400 ggt ttc tgg acc aag Gly Phe Trp Thr Lys 415 aag gca ctg cgt tcc Lys Ala Leu Arg Ser 405 cct gat gag ttc ttc Pro Asp Giu Phe Phe 420 WO 01/00843 WO 0100843PCT/IBOO/00923 gca ggg 1411 Ala Gly cgc cca 1459 Arg Pro ggc gca 1507 Gly Ala 455 ttC ctc 1555 Phe Leu 470 gac gca 1603 Asp Ala ggt ctg 1651 Gly Leu gaa gac 1699 Giu Asp ttc aag 1747 Phe Lys 535 tac cac 1795 Tyr His 550 gca cca 1843 Ala Pro aac cgc 1891 Asn Arg gag cgc Giu Arg 425 acc gaa Thr Giu gct acc gat aag gca Ala Thr Asp Lys Ala 430 ggc cgc ctc tac gac Gly Arg Leu Tyr Asp 445 gaa gaa ctc tac gaa Giu Giu Leu Tyr Giu gct gtt ctg gaa gat ctc aag Ala Val Leu Giu Asp Leu Lys 435 440 gtt gag ctg Val Giu Leu gca tct tct Ala Ser Ser agc gtg Ser Val 460 gcc gag ctt gaa gct Ala Giu Leu Giu Ala 465 ctc gtg cag ttc cgc Leu Val Gin Phe Arg 480 gat ctc ctg cgc gaa Asp Leu Leu Arg Giu gca atg cgc ctt Ala Met Arg Leu 450 att gat cct tgg Ile Asp Pro Trp cag aag ctc gtt Gin Lys Leu Val 485 gca aag ttc atg Ala Lys Phe Met 500 gag ttc gct ggc Glu Phe Ala Gly cca ttc cta Pro Phe Leu 490 tcc gac ctg Ser Asp Leu gaa Glu 505 ggc gta Gly Val 520 495 cag atc gca gcc Ctt Gin Ile Ala Ala Leu 510 acc ttg cgt ctg tcc Thr Leu Arg Leu Ser cgc cca Arg Pro cgc Arg 515 cta ggc atc cgc cca gta Leu Gly Ile Arg Pro Val 530 525 act gtg gat acc tgt Thr Val Asp Thr Cys 540 tac tcc gca tac gag Tyr Ser Ala Tyr Glu 555 cag act gag cgt gaa Gin Thr Glu Arg Glu gca Ala ctg Leu gca gag ttt gaa gct aag act ccg Ala Glu Phe Glu Ala Lys Thr Pro 545 gat cca gca gct gag tct gag gtc Asp Pro Ala Ala Glu Ser Glu Val 560 aag gtc ctg Lys Val Leu 575 atc Ile ttg ggc tcc ggt cca Leu Gly Ser Gly Pro 580 570 atc ggc Ile Gly 585 gct ctt gag ctc 1939 Ala Leu Giu Leu 600 cag ggc atc gag Gin Gly Ile Glu tcc cgc gtc ggc Ser Arg Val Gly 605 ttc gac tac tcc tgt gtt cac gca Phe Asp Tyr Ser Cys Val His Ala 590 595 tac gaa act gtc atg gtc aac tgc Tyr Giu Thr Val Met Val Asn Cys 610 WO 01/00843 WO 0100843PCTIiBOOIOO923 aac cca 1987 Asn Pro 615 ttc gag 2035 Phe Glu 630 gcg cag 2083 Ala Gin act cct 2131 Thr Pro att ggt 2179 Ile Gly ttc ggt 2227 Phe Gly 695 acc gca 2275 Thr Ala 710 tac cca 2323 Tyr Pro gag att 2371 Giu Ilie act gag 2419 Thr Glu aac gct 2467 Asn Ala 775 tac ctg 2515 Tyr Leu 790 gag acc gtg tcc acc Giu Thr Val Ser Thr 620 cca ctg acc ttc gaa Pro Leu Thr Phe Giu gac tac gac acc gct gac cgc ctg tac Asp Tyr Asp Thr Ala Asp Arg Leu Tyr 625 gac gtc atg gag gtc tac cac gct gag Asp Val Met Glu Val Tyr His Ala Giu 640 645 tcc ggc acc Ser Gly Thr 650 ctg ggc ttg Leu Gly Leu gca ggt gtt Ala Gly Val atc gtc cag ctt ggt ggc cag Ile Val Gin Leu Gly Giy Gin 655 660 aag aag gct ggc gtc cct gtc Lys Lys Ala Gly Val Pro Val gca gat cgt ttg Ala Asp Arg Leu 670 665 acc tcc Thr Ser 680 cca gag gca atc Pro Giu Ala Ile 685 gac atg gct gag gac Asp Met Ala Giu Asp 690 675 cgt ggc gag Arg Gly Glu gca ctg ctg aac cgc gag Ala Leu Leu Asn Arg Giu 700 cag ctt cct gct cca gca ttc ggc Gin Leu Pro Ala Pro Ala Phe Giy 705 cgc aca gta gcc gat gag atc agc Arg Thr Val Ala Asp Giu Ile Ser acc tct ttc gaa Thr Ser Phe Gl u 715 gtg ctg gtt cgc Vai Leu Val Arg 730 gag gct Giu Ala 720 725 cct tcc tac gtc Pro Ser Tyr Val 735 t tg Leu ga t Asp ggt ggc cgt ggc atg Gly Gly Arg Gly Met 740 tac atc aac cgc gca Tyr Ile Asn Arg Ala 755 gtc tac Vai Tyr 745 ttg tct Leu Ser 760 att gag Ile Glu gat gag gct tcc ctc Asp Giu Ala Ser Leu 750 tct gac cac cca gtg Ser Asp His Pro Val 765 gag Glu ctg gtt gac cgc ttc cta gac Leu Val Asp Arg Phe Leu Asp 770 atc gac gtc Ile Asp Val 780 gca ggc gtc atg gag Ala Gly Val Met Glu 795 gac gca ctg tgc gac ggc gac gag gtc Asp Ala Leu Cys Asp Gly Asp Giu Val 785 cac atc gag gaa gcc ggc att cac tcc His Ile Glu Giu Ala Gly Ile His Ser 800 805 WO 01/00843 WO 0100843PCTA[BOO/00923 ggt gac 2563 Gly Asp atc gag 2611 Ilie Glu gta cag 2659 Val Gin tac gtc 2707 Tyr Val 855 tcc aag 2755 Ser Lys 870 gtg ggc 2803 Val Gly gag tac 2851* Glu Tyr gaa gca 2899 Glu Ala ctg gac 2947 Leu Asp 935 ggc ttg 2995 Gly Leu 950 gcg ttt 3043 Ala Phe aac cgc 3091 Asn Arg tcc gca tgt Ser Ala Cys 810 aag gtc cgC Lys Val Arg gca ctt cct cca Ala Leu Pro Pro gaa gca acc aag Giu Ala Thr Lys 830 aac gtc cag tac Asn Val Gin Tyr 825 ggc ctg Gly Leu atg act ttg ggc gca cag gac met Thr Leu Gly Ala Gin Asp 815 820 aag ctg gct ctg ggc atc ggt Lys Leu Ala Leu Gly Ile Gly 835 gca ctc aag gac gac atc ctc Ala Leu LYS Asp Asp Ile Leu 850 tcc cgc acc gtg ccg ttc gtc Ser Arg Thr Val Pro Phe Val atg Met 845 gag gca aac cca Glu Ala Asn Pro 860 cgt gca Arg Ala gca acg ggc Ala Thr Gly gcc acc atc Ala Thr Ile 890 gac ggc ggc Asp Gly Gly 905 gtg ttg ccg Val Leu Pro gtC Val 875 aac Asn ctg gcc aag gca Leu Ala Lys Ala 880 gca tcc cgt atc gca Ala Ser Arg Ile Ala 885 ggc atg att cct acc Gly Met Ile Pro Thr 900 aag gat ctc caa Lys Asp Leu Gin tcc ttg cca ctg Ser Leu Pro Leu 910 ttc aac cgc ttc Phe Asn Arg Phe 925 tcc cca gag atg Ser Pro Giu Met ga t Asp 895 gag Glu gac gct cca atc gct gtg aag Asp Ala Pro Ile Ala Val Lys 915 cgt cgc cca gat gga aag acc Arg Arg Pro Asp Gly Lys Thr 930 aag tcc act ggc gag gtc atg Lys Ser Thr Gly Giu Val Met ctg ctt Leu Leu 940 gcc aac aac ttc Ala Asn Asn Phe 955 ggt gca ttg cca Gly Ala Leu Pro 970 gac aag cgc acc Asp Lys Arg Thr 985 ggC Gly acc Thr 945 gct gca tat gca aag Ala Ala Tyr Ala Lys 960 gct gaa gct ggc Ala Glu Ala Gly 965 gaa ggc acc Glu Gly Thr 975 ctg atc ctg cca Leu Ile Leu Pro 990 gtc ttc gtg acc gtg gct Val Phe Val Thr Val Ala 980 atc cag cgc ctg gcg ttg Ile Gin Arg Leu Ala Leu 995 WO 01/00843 WO 0100843PCTIiBOO/00923 atg ggc tac aag atc ctc gcc acc gaa ggc acc gca ggc atg ctg cgc 3139 Met Gly Tyr Lys Ilie Leu Ala Thr Giu Gly Thr Ala Gly Met Leu Arg 1000 1005 1010 cgc aac ggc att gag tgt gaa gtt gtg ctc aag gct tcc gac atc cgc 3187 Arg Asn Gly Ilie Glu Cys Giu Val Val Leu Lys Ala Ser Asp Ile Arg 1015 1020 1025 gaa ggt gta gag ggc aag tcc atc gtg gat cgt atc cgc gaa ggc gaa 3235 Giu Gly Val Giu Gly Lys Ser Ile Val Asp Arg Ile Arg Glu Gly Giu 1030 1035 1040 1045 gtt gac ctc atc ctc aac acc cca gct ggt tct gct ggc gct cgc cac 3283 Val Asp Leu Ile Leu Asn Thr Pro Ala Gly Ser Ala Gly Ala Arg His 1050 1055 1060 gat ggc tac gat atc cgc gca gca gca gtg acc gtg ggt gtt ccg ctg 3331 Asp Gly Tyr Asp Ile Arg Ala Ala Ala Val Thr Val Gly Val Pro Leu 1065 1070 1075 atc acc act gtt cag ggt gtc acc gca gct gtc cag ggc ata gag gcc 3379 Ile Thr Thr Vai Gin Gly Val Thr Ala Ala Val Gin Gly Ile Giu Ala 1080 1085 1090 ctg cgt gag ggc gtt gtc agc gtc cgc gcg ctg cag gaa ctc gac cac 3427 Leu Arg Giu Giy Val Val Ser Vai Arg Aia Leu Gin Giu Leu Asp His 1095 1100 1105 gca gtc aag gct taagccctat gacattcggc gag 3462 Ala Val Lys Ala 1110 <210> 368 <211> 1113 <212> PRT <213> Corynebacteriun giutamicun <400> 368 Met Pro Lys Arg Ser Asp Ile Asn His Val L4 1 5 10 Pro Ilie Val Ilie Gly Gin Ala Cys Giu Phe At 25 Aia Cys Arg Val Leu Lys Glu Giu Gly Leu Ai 40 Ser Asn Pro Ala Thr Ilie Met Thr Asp Pro G: 55 Tyr Val Glu Pro Ilie Giu Pro Glu Tyr Ile A! Val Ile Gly Ser Tyr Ser Gly Thr Val Thr Leu Ile Met Ala Asp His Lys Ile Phe Ala WO 01/00843 WO 0100843PCT/EBOO/00923 Glu Gly Leu Glu Gly 145 His Phe Leu Val1 Leu 225 Asn Pro Giy Ile Met 305 Gly Leu Glu Giu Val1 385 Giu Thr Lys 115 Gly Giu Thr Met Arg 195 Ile Arg Asp Leu Ala 275 Phe Pro Pro Glu Thr 355 Phe Glu His Asn Val1 Arg Arg 150 Glu Leu Gly Ser Ala 230 Gly Thr Arg Asn Ser 310 Lys Asn Tyr Ala Ser 390 Pro Ala Glu Gin 135 Ser Leu Gly Gly Ile 215 Asp Val1 Asp Giu Pro 295 Arg Met Asp Val1 Asp 375 Leu Ile Ala Leu 120 Lys Arg Gly Ser Gly 200 Leu Asn His Arg Val 280 Val Ser Ala Ile Val1 360 Asp Gly Asp Ile 105 Ile Phe Val Leu Gly 185 Leu Gly Val1 Thr Giu 265 Gly Asp Ser Ala Thr 345 Val1 Thr Arg Val1 Leu Ala Asp His 155 Val Ala Ala Lys Val 235 Asp Gin Asp Arg Leu 315 Leu Giu Ala Thr Tyr 395 Leu Asp Asp Ile 140 Asn Val1 Tyr Ser Glu 220 Ile Ser Lys Thr Ile 300 Ala Ala Thr Pro Thr 380 Ile Ala Arg Ile 125 Val Met Val Asn Pro 205 Phe Cys Val Met Giy 285 Ile Ser Ile Pro Arg 365 Thr Ala Thr Leu 110 Asp Thr Glu Arg Thr 190 Glu Giu Ser Thr Arg 270 Gly Thr Lys Giy Ala 350 Phe Met Ala Leu Gly Al a Thr Giu Pro 175 Giu Al a Leu Ile Val 255 Asp Cys Ile Ala Tyr 335 Ala Ala Lys Leu Gly Ile Ile Ile Val1 160 Ser Asp Asn Giu Giu 240 Ala Gin Asn Giu Thr 320 Thr Phe Phe Ser Asn 400 WO 01/00843 Lys Ala Le Pro Asp Gi Leu Glu As 43 Leu Ala Mc 450 Ser Ile As 465 Arg Gin Ly Giu Ala Ly Pro Giu PY 51 Gly Ile At 530 Giu Ala L) 545 Ala Giu SE Leu Gly SE Ser Cys Va Val Met Ve 610 Ala Asp Ax 625 Val Tyr Hi Gin Leu G2 Ala Gly Va Giu Asp At 690 Ala Pro Al 705 Ala Asp G] Arg Ser 405 Phe Phe 420 Leu Lys Arg Leu Pro Trp Leu Val 485 Phe Met 500 Ala Gly Pro Val Thr Pro Giu Val 565 Gly Pro 580 His Ala Asn Cys Leu Tyr Ala Giu 645 Gly Gin 660 Pro Val Gly Giu Phe Gly Ile Ser Gin 410 Ala Gly Giu Leu Leu 490 Leu Arg Asp Ala Gi'u 570 Gin Ser Val1 Thr Thr 650 Leu Pro Leu Phe Val1 Gly Asp Leu Leu 460 Ala Giu Ile Leu Cys 540 Giu Giu Ile Val Thr 620 Glu Ala Asp Ala Arg 700 Giu Pro Phe Lys Tyr 445 Tyr Leu Asp Ala Arg 525 Ala Leu Lys Glu Gly 605 Asp Asp Gly Arg Ile 685 Giu Ala Ser Trp Ala 430 Asp Glu Val1 Leu Ala 510 Leu Ala Asp Val Phe 590 Tyr Tyr Val Val Leu 670 Asp Gin Arg Tyr PCTlLBOO/00923 Thr Lys 415 Ala Val Val Glu Ala Ser Gin Phe 480 Leu Arg 495 Leu Arg Ser Leu Glu Phe Pro Ala 560 Leu Ile 575 Asp Tyr Giu Thr Asp Thr Met Giu 640 Ile Vai 655 Lys Lys Met Ala Leu Pro Thr Val 720 Val Leu WO 01/00843 Gly Gly Arg Tyr Ile Asn 755 Asp Arg Phe 770 Asp Gly Asp 785 Ala Gly Ile Leu Gly Ala Ala Leu Gly 835 Lys Asp Asp 850 Thr Val Pro 865 Ala Ser Arg Giy Met Ilie Pro Ilie Ala 915 Pro Asp Gly 930 Thr Gly Giu 945 Lys Ala Giu Phe Val Thr Gin Arg Leu 995 Ala Giy Met 1010 Ala Ser Asp 1025 Giy 740 Arg Leu Giu His Gin 820 Ile Ile Phe Ile Pro 900 Vali Lys Val1 Ala Vai 980 Al a Leu Ile 725 Met Aia Asp Val Ser 805 Asp Giy Leu Val Ala 885 Thr Lys Thr Met Gly 965 Aia Leu Arg Arg Giu Thr Asn Tyr 790 Gly Ile Val1 Tyr Ser 870 Vai Glu Glu Leu Gly 950 Ala Asn Met Arg Giu 103 Ile Val Tyr 745 Giu Leu Ser 760 Ala Ile Giu 775 Leu Ala Giy Asp Ser Ala Glu Lys Val 825 Gin Gly Leu 840 Val Ile Giu 855 Lys Ala Thr Gly Aia Thr Tyr Asp Giy 905 Ala Val Leu 920 Asp Thr Leu 935 Leu Ala Asn Phe Gly Ala Arg Asp Lys 985 Gly Tyr Lys 1000 Asn Gly Ilie 1015 Gly Val Giu 0 Asp Leu Ile Asp Glu Ala Ser Ser Asp His Pro 765 Ile Asp Val Asp 780 Val Met Glu His 795 Cys Ala Leu Pro 810 Arg Glu Ala Thr Met Asn Val Gin 845 Ala Asn Pro Arg 860 Giy Val Asn Leu 875 Ile Lys Asp Leu 890 Giy Ser Leu Pro Pro Phe Asn Arg 925 Leu Ser Pro Giu 940 Asn Phe Gly Ala 955 Leu Pro Thr Glu 970 Arg Thr Leu Ile Ile Leu Aia Thr 100 Glu Cys Giu Val 1020 Gly Lys Ser Ile 1035 Leu Asn Thr Pro 1050 PCTTIBOO/00923 735 Leu Giu Asp 750 Val Leu Val Ala Leu Cys Ile Giu Giu 800 Pro Met Thr 815 Lys Lys Leu 830 Tyr Ala Leu Aia Ser Arg Ala Lys Ala 880 Gin Asp Glu 895 Leu Asp Aia 910 Phe Arg Arg Met Lys Ser Ala Tyr Ala 960 Gly Thr Val 975 Leu Pro Ile 990 Giu Gly Thr Val Leu Lys Val Asp Arg 1040 Ala Gly Ser 1055 Ile Arg Giu Gly Giu Val 1045 WO 01/00843 WO 0100843PCT/iBOO/00923 Ala Gly Ala Arg His 1060 Asp Gly Tyr Asp Ile Arg Ala Ala Ala Val Thr 1065 1070 Val Gly Val Pro Leu Ile Thr Thr Val 1075 1080 Gin Gly Ile Giu Ala Leu Arg Glu Gly 1090 1095 Gin Giu Leu Asp His Ala Val Lys Ala 1105 1110 <210> 369 <211> 3221 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (1)..(3198) <223> FRXA02234 Gin Gly Val Thr Ala Ala Val 1085 Val Val Ser Val Arg Ala Leu 1100 <400> 369 ggc tcc aac cca Gly Ser Asn Pro 1 gca Ala 5 acg atc atg acc gac cca gaa atg gct Thr Ile Met Thr Asp Pro Giu Met Ala 10 gac cac Asp His acc tac gtg Thr Tyr Val aag gaa atc Lys Glu Ilie cca atc gag ccg Pro Ile Glu Pro tac atc gac aag Tyr Ile Asp Lys att ttc gct Ile Phe Ala gca acc ctt Ala Thr Leu gag cag ggc cac Giu Gin Gly His atc gac gcc gtc Ile Asp Ala Val ctg Leu ggt ggc Gly Gly cag act gca ctt Gin Thr Ala Leu gca gct atc cag ctg gat cgc ctc ggc Ala Ala Ile Gin Leu Asp Arg Leu Gly ctg gaa aag tac Leu Giu Lys Tyr gtt gaa ctc atc Val Giu Leu Ilie gca gac atc gat Ala Asp Ile Asp att gag cgc ggc Ilie Giu Arg Gly gaa Giu gat cgc cag aag Asp Arg Gin Lys aag gat att gtc Lys Asp Ile Val acc acc Thr Thr atc ggt ggc Ilie Gly Gly gtc cac gag Val His Giu 115 tcC gcg cgt tcc Ser Ala Arg Ser gtc tgc cac aac atg gaa gaa Val Cys His Asn Met Giu Giu 110 act gtc gca gaa Thr Val Ala Glu ggc ctt cca gta Gly Leu Pro Val gtg cgt cca Val Arg Pro tcc ttC Ser Phe 130 act atg ggt ggc Thr Met Gly Gly ggc tcc ggt ctt Gly Ser Gly Leu tac aac acc gaa Tyr Asn Thr Giu WO 01/00843 gac ctt Asp Leu 145 aac gtc Asn Val gag ctc Glu Leu gaa aac Glu Asn gca cct Ala Pro 210 cag ggt Gin Gly 225 aac atc Asn Ile gag atg Giu Met acg ggc Thr Gly acc ctg Thr Leu 290 .ttt gag Phe Giu 305 ttc gag 1008 Phe Glu tcc gtc 1056 Ser Val aac aag 1104 Asn Lys aag cct 1152 gag cgc atc gct Giu Arg Ile Ala 150 ggt ggc gga ctt gct gca tct cct Gly Gly Gly Leu Ala Ala Ser Pro 155 atc Ile cgc Arg 180 gac Asp c tg Leu gcc Ala ttc Phe cca Pro 260 cca Pro gag Giu acc Thr ttt Phe gag Glu 340 ctg Leu gag gaa Giu 165 ga t Asp gca Al a acc Thr atc Ile gcc Ala 245 cgt Arg att Ile at c Ile atc Ile gtc Val 325 gtc Val cgt Arg t tc aag Lys gtt Val gac Asp cag Gin 220 gac Asp cgc Arg c tg Leu ctg Leu gaa Giu 300 gcc Ala acc Thr tac Tyr cag Gin acc gaa Giu atc Ile tct Ser 205 aag Lys acc Thr atc Ile gca Ala gc t Ala 285 acc Thr cca Pro acc Thr att Ile ggt Giy 365 gat PCTIIBOO/00923 gaa gca 480 Giu Ala 160 gag ctc 528 Giu Leu 175 tcc att 576 Ser Ile acc gtg 624 Thr Vai cgc gat 672 Arg Asp gga tgt 720 Gly Cys 240 acc att 768 Thr Ile 255 aag gca 816 Lys Ala gga tac 864 Gly Tyr gct gcg 912 Ala Ala ttt gct 960 Phe Ala 320 atg aag Met Lys 335 gca ctg Ala Leu tgg acc Trp Thr gca gct WO 01/00843 WO 0100843PCT/IBOO/00923 Lys Pro 370 gtt ctg 1200 Val Leu 385 gag ctg 1248 Giu Leu tct tct 1296 Ser Ser ttc cgc 1344 Phe Arg cgc gaa 1392 Arg Glu 450 cgc cca 1440 Arg'Pro 465 cta ggc 1488 Leu Giy ttt gaa 1536 Phe Giu gca gct 1584 Ala Ala atc ttg 1632 Ilie Leu 530 tac tcc 1680 Tyr Ser 545 act gtc 1728 Thr Val Asp Giu Phe Phe gaa gat ctc aag Giu Asp Leu Lys 390 gca atg cgc ctt Ala Met Arg Leu Ala 375 cgc Arg Gly Giu Arg Ala cca acc gaa ggc Pro Thr.Glu Gly 395 Asp Lys Ala Ala ctc tac gac gtt Leu Tyr Asp Val 400 ggc gca agc gtg Gly Ala Ser Val 410 gaa gaa ctc tac gaa gca Glu Giu Leu Tyr Giu Ala 415 ctt gaa gct ctc gtg cag Leu Glu Ala Leu Val Gin 430 att gat Ilie Asp 420 cag aag Gin Lys tgg ttc ctc Trp Phe Leu gtt gac gca gcc Ala 425 gag Glu c tc 435 gca Ala aag Lys 440 ttc atg ggt ctg Phe Met Giv Leu cca ttc cta aac gaa gat ctc ctg Pro Phe Leu Asn Glu Asp Leu Leu 445 tcc gac ctg cag atc gca gcc ctt Ser Asp Leu Gin Ile Ala Ala Leu 455 gag ttc gct Glu Phe Ala atc cgc cca Ile Arg Pro 485 gct aag act Ala Lys Thr ggc Gly 470 gaa gac ggc gta cgc Glu Asp Gly Val Arg 475 acc ttg cgt ctg tcc Thr Leu Arg Leu Ser 480 acc tgt gca gca gag Thr Cys Ala Ala Giu 495 gta ttc aag act gtg Val Phe Lys Thr Val 490 ccg tac cac tac tcc Pro Tyr His Tyr Ser 505 gtc gca cca cag act Val Ala Pro Gin Thr 520 gat Asp gca tac gag ctg gat cca A14 Tyr Giu Leu Asp Pro 510 500 gag tct Glu Ser gag Giu gag Glu tcc ggt cca Ser Gly Pro tgt gtt cac gca Cys Val His Ala 550 atg gtc aac tgc Met Val Asn Cys aac cgc atc ggc cag Asn Arg Ile Gly Gin 535 gct ctt gag ctc tcc Ala Leu Glu Leu Ser 555 aac cca gag acc gtg Asn Pro Glu Thr Val cgt gaa aag gtc ctg Arg Giu Lys Val Leu 525 ggc atc gag ttc gac Gly Ile Glu Phe Asp 540 cgc gtc ggc tac gaa Arg Val Gly Tyr Giu 560 tcc acc gac tac gac Ser Thr Asp Tyr Asp WO 01/00843 WO 0100843PCT/iBOO/00923 acc gct 1776 Thr Ala gag gtc 1824 Glu Val gtc cag 1872 Val Gin 610 aag gct 1920 Lys Ala 625 gct gag 1968 Ala Glu cct gct 2016 Pro Ala gta gcc 2064 Val Ala ttg ggt 2112 Leu Gly 690 gat tac 2160 Asp Tyr 705 gtt gac 2208 Val Asp tgc gac 2256 Cys Asp gaa gcc 2304 Giu Ala gac cgc Asp Arg 580 tac cac Tyr His 565 c tg Leu gc t Ala tac ttc gag cca Tyr Phe Giu Pro 585 gag gcg cag tcc Giu Ala Gin Ser 570 575 ctg acc ttc gaa gac gtc atg Leu Thr Phe Glu Asp Val Met 590 ggc acc Gly Thr 595 ctt Leu ggC Giv 600 ggt ggc cag Gly Gly Gin gtc cct gtC Val Pro Val act Thr 615 cct Pro ctg ggc ttg Leu Gly Leu 630 gac cgt ggc Asp Arg Gly 645 cca gca ttc Pro Ala Phe gag Glu ggc Gly att ggt acc tcc cca Ile Gly Thr Ser Pro 635 ttc ggt gca ctg ctg Phe Gly Ala Leu Leu 650 acc gca acc tct ttc Thr Ala Thr-Ser Phe gtc gca ggt gtt atc Val Ala Gly Val Ile 605 gca gat cgt ttg aag Ala Asp Arg Leu Lys 620 gag gca atc gac atg Glu Ala Ile Asp Met 640 aac cgc gag cag ctt Asn Arg Glu Gin Leu 655 gaa gag gct cgc aca Glu Glu Ala Arg Thr 660 gat gag Asp Glu 665 atc agc tac cca gtg Ile Ser Tyr Pro Val 670 ctg gtt cgc cct tcc tac gtc Leu Val Arg Pro Ser Tyr Val 685 tac gat gag gct tcc ctc gag Tyr Asp Giu Ala Ser Leu Glu ggc cgt ggc atg gag Gly Arg Gly Met Giu 695 atc aac cgc gca act Ile Asn Arg Ala Thr att gtc Ile Val gag ttg tct tct Glu Leu Ser Ser cgc ttc cta Arg Phe Leu 725 ggc gac gag Gly Asp Glu 710 gac aac Asp Asn gct att gag Ala Ile Giu 730 715 atc Ile 700 gac cac cca gtg ctg Asp His Pro Val Leu 720 gac gtc gac gca ctg Asp Val Asp Ala Leu 735 gtc tac ctg Val Tyr Leu 740 ggc att Gly Ilie 755 gca ggc gtc atg gag cac atc gag Ala Gly Val Met Giu His Ile Giu 745 750 tcc gca tgt gca ctt cct cca atg Ser Ala Cys Ala Leu Pro Pro Met 765 cac tcc ggt gac His Ser Gly Asp 760 WO 01/00843 WO 0100843PCTJIBOO/00923 act ttg 2352 Thr Leu 770 ctg gct 2400 Leu Ala 785 ctc aag 2448 Leu Lys cgc acc 2496 Arg Thr gca gca 2544 Ala Ala gag ggc 2592 Giu Giy 850 gct cca 2640 Ala Pro 865 cgc cca 2688 Arg Pro tcc act 2736 Ser Thr gca aag 2784 Ala Lys gtc ttc 2832 Val Phe 930 atc cag 2880 Ile Gln 945 ggc gca cag gac Giy Ala Gin Asp ctg ggc atc ggt Leu Gly Ilie Gly 790 gag aag gtc cgc gaa gca acc aag aag Giu Lys Val Arg Glu Ala Thr Lys Lys 780 cag ggc ctg atg aac gtc cag tac gca Gin Gly Leu Met Asn Vai Gin Tyr Ala gta Vali 795 800 gac gac Asp Asp gtg ccg Val Pro 820 tcC cgt Ser Arg 835 atc ctc tac gtc atc gag Ile Leu Tyr Val Ilie Giu 805 810 ttc gtc tcc aag gca acg Phe Vai Ser Lys Ala Thr 825 atc gca gtg ggc gcc acc Ilie Ala Vai Gly Ala Thr .840 gca aac cca cgt gca tcc Ala Asn Pro Arg Ala Ser 815 ggc gtc aac ctg gcc aag Giy Val Asn Leu Ala Lys 830 atc aag gat ctc caa gat Ile Lys Asp Leu Gin Asp 845 ggc tcc ttg cca ctg gac Gly Ser Leu Pro Leu Asp atg att cct acc gag Met Ile Pro Thr Giu 855 tac gac ggc Tyr Asp Giy atc gct gtg aag Ile Ala Val LYS 870 gat gga aag acc Asp Gly Lys Thr 885 ggc gag gtc atg Giv Giu Val Met gaa Giu ctg 860 gca gtg ttg ccg ttc Ala Vai Leu Pro Phe 875 gac acc ctg ctt tcc Asp Thr Leu Leu Ser aac cgc ttc cgt Asn Arg Phe Arg 880 cca gag atg aag Pro Giu Met Lys 895 ggc ttg gcc Giy Leu Ala 890 900 gc t Ala 915 gtg Val gaa gct ggc gcg ttt Giu Ala Gly Ala Phe 920 acc gtg gct aac cgc Thr Vai Ala Asn Arg 935 aac aac ttc ggc gct gca tat Asn Asn Phe Gly Ala Aia Tyr 910 gca ttg cca acc gaa ggc acc Ala Leu Pro Thr Giu Gly Thr 925 aag cgc acc ctg atc ctg cca Lys Ara Thr Leu Ile Leu Pro gac Asp cgc .ctg Arg Leu gcg ttg Aia Leu 950 atg ggc Met Gly 940 tac aag atc ctc Tyr Lys Ile Leu 955 gcc acc gaa ggc Ala Thr Glu Gly 960 WO 01/00843 acc gca ggc 2928 Thr Ala Giy aag gct tcc 2976 Lys Ala Ser cgt atc cgc 3024 Arg Ile Arg 995 tct gct ggc 3072 Ser Ala Gly 1010 acc gtg ggt 3120 Thr Val Giy 1025 gtc cag ggc 3168 Val Gin Gly ctg cgc cgc aac ggc att g Leu Arg Arg Asn Gly Ile G 965 970 atc cgc gaa ggt gta gag g9 Ile Arg Giu Gly Val Giu G 985 ggc gaa gtt gac ctc atc ci Gly Glu Val Asp Leu Ile L4 1000 cgc cac gat ggc tac gat ai Arg His Asp Gly Tyr Asp I 1015 ccg ctg atc acc act gtt c( Pro Leu Ile Thr Thr Val G~ 1030 gag gcc ctg cgt gag ggc gi Giu Ala Leu Arg Giu Giy V 1045 1050 ag tgtgaa gtt Lu Cys Glu Val gc aag tcc atc ly Lys Ser Ile 990 tc aac acc cca eu Asn Thr Pro 1005 tc cgc gca gca le Arg Aia Aia 1020 ag ggt gtc acc Ln Gly Val Thr 1035 tt gtc agc gtc PCTIBOOIOO923 gtg ctc Val Leu 975 gtg gat Val Asp gct ggt Aia Giy gca gtg Ala Vai gca gct Ala Aia 1040 cgc gcg al Vai Ser Val Arg Aia 1055 ctg cag 3218 gaa ctc gac cac gca gtc aag gct taagccctat gacattcggc Leu Gin Giu Leu Asp His Ala Val Lys Ala 1060 106 gag 3221 <210> 370 <21i> i066 <212>. PRT <213> Corynebacteriun glutamicum <400> 370 Gly Ser Asn Pro Ala Thr Ile Met Thr 1 5 Thr Tyr Val Giu Pro Ile Glu Pro Giu Lys Giu Ilie Glu Gin Giy His Pro Ile Gly Gly Gin Thr Ala Leu Asn Ala Ala Ile Leu Glu Lys Tyr Gly Val Giu Leu 70 Ile Glu Arg Gly Giu Asp Arg Gin Lys 556 Asp Phe Thr Leu Asp Thr His Ala Leu Gly Ala Thr WO 01/00843 Ile Vai S er Asp 145 Asn Giu Glu Ala Gin 225 Asn Giu Thr Thr Phe 305 Phe Ser Asn Lys Val1 385 Glu Giy His Phe 130 Leu Vai Leu Asn Pro 210 Gly Ile Met G ly Leu 290 Giu Giu Val1 Lys Pro 370 Leu Leu Giy Giu 115 Thr Giu Leu Met Val1 195 Ala Ile Gin Asn Phe 275 Asp Pro Lys Giy Ala 355 Asp Giu Ala Ser Val1 Giy Ile Glu 165 Asp Ala Thr Ile Ala 245 Arg Ile Ile Ile Val 325 Val1 Arg Phe Leu Arg Ala Ala Gly Ala 150 Giu Thr Leu Leu Ile 230 Ile Val1 Ala Thr Asp 310 Gly Met Ser Phe Lys 390 Leu Asp Asn 185 Val His 200 Asp Arg Glu Val Pro Val Arg Ser 265 Met Ala 280 Asp -le Vai Vai Asp Asp Leu Gly 345 Giu Thr 360 Gly Giu Pro Thr Ala Ser 90 Val Leu Giy Leu Gly 170 Vai Thr Glu G ly Asp 250 Ser Ala Thr Val Thr 330 Arg Lys Arg Glu Val1 Cys Pro Leu Ala 155 Trp Val Gly Phe Val 235 Gly Ala Lys G ly Lys 315 Leu Asn Gin Ala Giy 395 Glu His Val1 Ala 140 Ala Lys Val Asp Gin 220 Asp Arg Leu Leu Giu 300 Al a Thr Tyr Gin Thr 380 Arg Glu Asn Val1 125 Tyr Ser Glu Ile Ser 205 Lys Thr Ile Ala Ala 285 Thr Pro Thr Ile Gly 365 Asp Leu Leu Met 110 Val1 Asn Pro Phe Cys 190 Val Met Gly Ile Ser 270 Ile Pro Arg Thr Ala 350 Phe Lys Tyr Tyr PCTIBOO/00923 Glu Glu Arg Pro Thr Glu Glu Ala 160 Glu Leu 175 Ser Ile Thr Val Arg Asp Giy Cys 240 Thr Ile 255 Lys Ala Gly Tyr Aia Ala Phe Ala 320 Met Lys 335 Ala Leu Trp Thr Ala Ala Asp Val 400 Glu Ala 415 WO 01/00843 WO 0100843PCTIBOO/00923 Ser Phe Arg Arg 465 Leu Phe Ala Ile Tyr 545 Thr Thr Giu Val Lys 625 Ala Pro Val Leu Asp 705 Val1 Ser Arg Giu 450 Pro Gly Glu Ala Leu 530 Ser Val1 Ala Val Gin 610 Ala Glu Ala Ala Gly 690 Tyr Asp Asp 420 Lys Lys Phe Arg Lys 500 S er Ser Val Val Arg 580 His Gly Val1 Arg Ala 660 Giu Arg Asn Phe Pro Leu Phe Ala Pro 485 Thr Giu Gly His Asn 565 Leu Ala Gly Pro Gly 645 Phe Ile Gly Arg Leu 725 Trp, Val1 Met Gly 470 Val Pro Val1 Pro Ala 55.0 Cys Tyr Giu Gin Val1 630 Giu Gly Ser Met Ala 710 Asp Phe Asp Gly 455 Glu Phe Tyr Ala Asn 535 Ala Asn Phe Ala Thr 615 Ile Phe Thr Tyr Giu 695 Thr Asn Leu Ala 440 Leu Asp Lys Hius Pro 520 Arg Leu Pro Giu Gin 600 Pro Gly Gly Ala Pro 680 I le Glu Ala Ala 425 Pro Ser Gly Thr Tyr 505 Gin Ile Giu Giu Pro 585 Ser Leu Thr Ala Thr 665 Val1 Val Leu Ile Leu Leu Leu Arg 475 Asp Ala Giu Gin Ser 555 Val Thr Thr Leu Pro 635 Leu Phe Val Asp Ser 715 Ile Giu Asn Gin 460 Thr Thr Tyr Arg Gly 540 Arg Ser Phe Val1 Ala 620 Giu Asn Giu Arg Giu 700 Asp Asp Ala Glu 445 Ile Leu Cys Giu Glu 525 Ile Val Thr Glu Ala 605 Asp Ala Arg Giu Pro 685 Ala His Val1 Leu 430 Asp Ala Arg Ala Leu 510 Lys Giu Gly Asp Asp 590 Gly Arg Ile Glu Ala 670 Ser Ser Pro Asp Val1 Leu Ala Leu Ala 495 Asp Val1 Phe Tyr Tyr 575 Val Val Leu Asp Gin 655 Arg Tyr Leu Val1 Ala 735 Gln Leu Leu Ser 480 Gi u Pro Leu Asp Giu 560 Asp Met Ile Lys Met 640 Leu Thr Val1 Glu Leu 720 Leu WO 01/00843 Cys Asp Gly Giu Ala Gly 755 Thr Leu Gly 770 Leu Ala Leu 785 Leu Lys Asp Arg Thr Val Ala Ala Ser 835 Giu Gly Met 850 Ala Pro Ilie 865 Arg Pro Asp Ser Thr Gly Ala Lys Ala 915 Val Phe Val 930 Ile Gin Arg 945 Thr Ala Gly Lys Ala Ser Arg Ilie Arg 995 Ser Ala Giy 1010 Thr Val Gly 1025 Val Gin Gly PCTIBOO/00923 Gly Val Met Glu His Ile Giu 750 Asp Giu Val Tyr Leu Ala 740 745 Ile Ala Gly Asp Pro 820 Arg Ile Ala Gly Giu 900 Giu Thr Leu Met Asp 980 Giu Ala Val1 Ile His Gin Ile Ile 805 Phe Ile Pro Val1 Lys 885 Val Ala Val1 Ala Leu 965 Ilie Gly Arg Pro Glu Ser Gly Asp Ser 760 Asp Ilie Giu Lys 775 Gly Val Gin Gly 790 Leu Tyr Val Ile Val Ser Lys Ala 825 Ala Val Gly Ala 840 Thr Giu Tyr Asp 855 Lys Giu Ala Val 870 Thr Leu Asp Thr Met Gly Leu Ala 905 Gly Ala Phe Gly 920 Ala Asn Arg Asp 935 Leu Met Gly Tyr 950 Arg Arg Asn Gly Arg Glu Gly Val 985 Glu Val Asp Leu 1000 His Asp Gly Tyr 1015 Leu Ile Thr Thr 1030 Ala Leu Arg Glu Leu Pro 765 Ala Thr Val Gin Pro Arg Asn Leu 830 Asp Leu 845 Leu Pro Asn Arg Pro Glu Gly Ala 910 Thr Glu 925 Leu Ile Ala Thr Giu Val Ser Ile 990 Thr Pro 1005 Ala Ala Pro Lys Tyr Ala 815 Ala Gin Leu Phe Met 895 Ala Gly Leu Giu Val1 975 Val1 Ala Ala 1020 Gin Giy Val Thr Ala Aia 1035 Gly Val Val Ser 1050 1040 Val Arg Ala 1055 1045 Leu Gin Glu Leu Asp His Ala Val Lys Ala WO 01/00843 WO 0100843PCTIiBOO/00923 <210> <211> <212> <213> <220> <221> <222> <223> 1060 371 1389

DNA

Corynebac terium

CDS

(101)..(1366) RXS0 2565 1065 giu tami cum <400> 371 ggaaattcga tacagtgcga tgacgcgata ttagaaagaa aaagatgcgc tttacgacga aaccctcacc ctccttcagg aacttatccg caacgcctgc gtg aat gat cta Val Asn Asp Leu cca gat tca ggt cag gaa att aga aac Pro Asp Ser Gly Gin Glu Ile Arg Asn gaa agc cta gaa Giu Ser Leu Giu cgt ttc Arg Phe ttt gaa gga Phe Giu Giy ccc aac gtt aaa Pro Asn Val Lys acc aag ctg gaa ccg cat ccg Thr Lys Leu Giu Pro His Pro ggc cgg acc tca att atc gtg Gly Arg Thr Ser Ile Ile Val act Thr gtt cca ggc agc Val Pro.Gly Ser cca gat gct Pro Asp Aia gag cct tta aca ctg ct Glu Pro Leu Thr Leu Leu gga Gly cat act gat gtt gtg cct gtt gat ctg His Thr Asp Val Val Pro Val Asp Leu cc C Pro aaa tgg act aaa Lys Trp, Thr Lys cca ttc ggt gcg gag att tcg gat gga Pro Phe Gly Ala Giu Ile Ser Asp Gly att tgg ggt Ile Trp Giy gcg gcc gtc Aia Aia Val ctg aca ttc Leu Thr Phe 120 aga Arg tcc gtc gat atg ctc CCC att acc gca Ser Vai Asp Met Leu Phe Ile Thr Ala acc caa Thr Gin 100 acc cgt caa gta gcc cgt gaa ggc ggc ctg cgt ggc acg Thr Arg Gin Vai Aia Arg Glu Gly Giy Leu Arg Gly Thr 105 110 115 gtt ggc gtt gct Val Gly Val Ala gatC Asp 125 gag gaa gcc cgc Giu Giu Ala Arg gga ctc gga Gly Leu Giy gcg aag tgg ctt Ccc gaa gaa cac caa aac ctc Ctc agc tgg aaa aac Aia Lys Trp Leu Ser Glu Glu His Gin Asn Leu Phe Ser Trp Lys Asn 135 140 145 ctc Ccc gaa tcc Leu Ser Giu Ser ggt Gly 155 gga Ccg cac cCC Gly Ser His Leu gtc cac gac ggc Val His Asp Gly gac gca gta gta att aac gtt gga gaa aaa ggt gca gct caa cgt cgt WO 01/00843 PCTIiBOO/00923 Val Ile Asn Val Gly Glu Lys Gly Ala Ala Gin Arg Arg Asp Ala att cac Ile Hius gac agc Asp Ser gcc gat Ala Asp 215 gcg cac Ala His 230 tcc cct Ser Pro gcg gtg Ala Val gcc atc Ala Ilie acc ctt 1027 Thr Leu 295 gct ctg 1075 Ala Leu 310 gaa gaa 1123 Giu Glu gaa aaa 1171 Glu Lys att tcc 1219 Ilie Ser gtt ggt 1267 Val Gly 375 Val1 gtc Val gc t Ala 200 ctg Leu cgt Arg gag Giu tc t Ser aa t Asn 280 cc a Pro ggc Gly gca Ala gtt Val1 tct Ser 360 tat Tyr 170 175 180 ggc Gly gtc Val gta Val1 gac Asp tac Tyr 250 cc Leu t tg Leu caa Gin ctt Leu gtg Val 330 ggt Gly ggc Gly t tt Phe gat Asp aag Lys gcc Ala cca Pro 235 gca Ala acg Thr cca Pro ac c Thr gcc Ala 315 agc Ser gat Asp tct Ser gca Ala gc t Ala atc Ile aag Lys 220 gaa Giu gag Giu atc Ile tcg Ser aat Asn 300 gat Asp cca Pro ttc Phe gac Asp gtt Vali 380 gg t Gly ggt Gly 205 gac Asp acg Thr ttc Phe gcc Aia cat His 285 gac Asp gaa Glu act Thr ttc Phe c tg Leu 365 cat His cat His 190 gaa Glu gat Asp gag Giu Gly caa Gin 270 gcg Aia tat Tyr gta Val gat Asp ccc Pro 350 cgc Arg gca Ala ggt Gly gtc Val atc Ile cag Gin gga Gly 255 act Thr tac Tyr gtt Val gaa Glu tcc Ser 335 gat Asp t t Phe cgt Arg tcc Ser gcc Aia tgg Trp gcg Ala 240 c tc Leu gtg Val1 ttg Leu gat Asp atc Ile 320 agg Arg gcg Ala ggt Gly gaa Glu t tc Phe 195 atc Ile ttc Phe agc Ser ttc Phe gca Ala 275 gat Asp ctg Leu ctc Leu aac Asn gtc Vai 355 c ta Leu tg Leu gac Asp gct Ala gtc Val1 ggg Gly gcc Ala 260 ggt Giy atc Ile cgt Arg atc Ile acc Thr 340 cca Pro ggc Gly gcg Ala WO 01/00843 PCT/iB00100923 gca atg ggg caa ctt cac tcc cat gac gag gcg ctg tac ctg gaa gat 1315 Ala Met Gly Gin Leu His Ser His Asp Giu Ala Leu Tyr Leu Glu Asp 390 395 400 405 ctt gaa ctg act gtt cgg ggt tat gac tcc gtc gtg cgt gaa ttc Cta 1363 Leu Glu Leu Thr Val Arg Gly Tyr Asp Ser Val Val Arg Glu Phe Leu 410 415 420 ggc taaaaacatg aagcaggagt ctt 1389 Gly <210> 372 <211> 422 <212> PRT <213> Corynebacterium glutanicun <400> 372 Val Asn Asp Leu Thr Pro Asp Ser Gly Gin Glu Ile Arg Asn Ala Glu 1 5 10 Ser Leu Glu Arg Phe Phe Glu Gly Thr Pro Asn Val Lys Ile Thr Lys 25 Leu Glu Pro His Pro Gly Arg Thr Ser Ile Ile Val Thr Val Pro Gly 40 Ser Asp Pro Asp*Ala Glu Pro Leu Thr Leu Leu Gly His Thr Asp Val 55 Val Pro Val Asp Leu Pro Lys Trp, Thr Lys Asp Pro Phe Gly Ala Glu 70 75 Ile Ser Asp Gly Gin Ile Trp Gly Arg Gly Ser Val Asp Met Leu Phe 90 Ilie Thr Ala Thr Gin Ala Ala Vai Thr Arg Gin Val Ala Arg Glu Gly 100 105 110 Gly Leu Arg Gly Thr Leu Thr Phe Val Gly Val Ala Asp Giu Giu Ala 115 120 125 Arg Gly Gly Leu Gly Ala Lys Trp Leu Ser Glu Giu His Gin Asn Leu 130 135 140 Phe Ser Trp Lys Asn Cys Leu Ser Giu Ser Gly Gly Ser His Leu Pro 145 150 155 160 Vai His Asp Gly Ser Asp Ala Val Val Ile Asn Val Gly Giu Lys Gly 165 170 175 Ala Ala Gln Arg Arg Ile His Val Asn Gly Asp Ala Gly His Gly Ser 180 185 190 Ile Pro Phe Asp Arg Asp Ser Ala Ile Val Lys Ile Gly Glu Val Ala 195 200 205 WO 01/00843 WO 0100843PCTIIBOOIO0923 Arg Arg Ile Ala Ala Ala Asp Leu Lys Val Ala Lys Asp Asp Ile Trp, 210 215 220 Gln 225 Leu Ser Val Glu Asp 305 Glu Leu Pro Arg Arg 385 Leu Val1 Gly Leu Arg Arg Leu 290 Thr His Tyr Val1 Arg 370 Thr Tyr Arg Phe Ser Phe Ala 275 Asp Leu Leu Asn Val 355 Leu Leu Leu Giu Val1 Gly Ala 260 Gly Ile Arg Ile Thr 340 Pro Gly Ala Giu Phe 420 Gin Thr 245 His Gin Arg Al a Ser 325 Leu Ile Gly Giu Asp 405 Leu Ala 230 Ser Ala Ala Thr Ala 310 Giu Giu Ile Val1 Aia 390 Leu Giy His Pro Val Ile Leu 295 Leu Glu Lys Ser Gly 375 Met Glu Arg Giu Ser Asn 280 Pro Giy Ala Val1 Ser 360 Tyr Gly Leu Phe Ala His 265 Vai Gly Asp Thr Leu 345 Gly Giy Gin Thr Asp Tyr 250 Leu Leu Gin Leu Val1 330 Gly Gly Phe Leu Val 410 Pro 235 Ala Thr Pro Thr Ala 315 Ser Asp Ser Ala His 395 Arg Glu Glu Ile Ser Asn 300 Asp Pro Phe Asp Val 380 Ser Gly Thr Phe Ala His 285 Asp Giu Thr Phe Leu 365 His His Tyr Gin Gly 255 Thr Tyr Val1 Glu Ser 335 Asp Phe Arg Giu Ser 415 Ala 240 Leu Val1 Leu Asp Ile 320 Arg Aia Giy Giu Ala 400 Val1 <210> 373 <211> 525 <212> DNA <213> Corynebacteriun glutainicum <22 0> <221> CDS <222> (101)..(502) <223> RXS02937 <400> 373 gctaccgcga agaactgtac tagttcttcc atcagcaccc gcagttgtct ttgcaagagt tcgaaacatc caacgcattg gtgacaccgg tgttgtcgcg gtg atc agc aat ggg 115 Val Ile Ser Asn Gly 1 gaa ggt ccg gtt gtt gcg ctt cgt ggc gac att gat gcg ttg ccc atg 163 563 WO 01/00843 Giu Gly Pro gcg gag cga Ala Giu Arg gat aac acc Asp Asn Thr gat gtg cat Asp Val His cat cgg gaa His Arg Giu gaa gag acg Giu Giu Thr ccg gga aat Pro Giy Asn gat cgt ggt Asp Arg Gly 120 Val Val tcc ggc Ser Gly 25 acc ggt Thr Gly att tca Ilie Ser ttg tgg Leu Trp gca gct Ala Ala cac tct His Ser 105 gtg gaa Val Glu Ala Leu Arg aaa gaa tac Lys Glu Tyr caa gaa act Gin Giu Thr tca ctg ttg Ser Leu Leu aac gga acg Asn Gly Thr 75 ggt gcg agg Gly Ala Arg cca gcg ttc Pro Ala Phe gcg ctg gtt Ala Leu Val 125 Leu aca Thr tgt Cys ttc Phe cag Gin gac Asp cca Pro 115 tgg Trp PCTIBOO/00923 Pro Met cag gtg 211 Gin Vai ggc cac 259 Gly His aat tct 307 Asn Ser cca gcg 355 Pro Ala aac gcg 403 Asn Ala 100 act ctt 451 Thr Leu cta gta 499 Leu Val taattggcta atgaatcctt <210> 374 <211> 134 <212> PRT <213> Corynebacteriun giutamicum <400> 374 Val Ilie Ser Asn Giy Giu Gly Pro Val 1 5 Asp Ala Leu Pro Met Ala Giu Arg Ser 25 Gly Val Thr Gin Vai Asp Asn Thr Thr His Thr Cys Giy His Asp Vai His Ile Gin Ala Phe Asn Ser His Arg Giu Leu 70 Vai Phe Gin Pro Ala Giu Giu Thr Ala Asp Gin Asp Asn Ala Pro Giy Asn His 564 Gly Ala Pro Gly Leu Met Ala Asp Ala Val Ala Met Met Pro Ile Thr Ala Val Ala Ala Asp WO 01/00843 WO 0100843PCTIIBOOIOO923 110 Val Ala Ala Met Gin Pro Thr Leu Asp Arg Gly Val Glu Ala Leu Val 115 120 125 Ser Ala Trp, Leu Val Lys 130 <210> 375 <211> 966 <212> DNA <213> Corynebacterium <22 0> <221> CDS <222> (943) <223> RXA02194 <400> 375 gaaatctccc agctcattta glutamicum ttggacccag gtcatcatgg ttgctcgcgg cctgaagcca gaagatatct acaagaacct gtaggagttt taaagcaarc atg Met 1 ttg aaa atc Leu Lys Ile gtc cca aac aaa Val Pro Asn Ly, tcg ctg tcc gag Ser Leu Ser Glu gcc arg gaa atc Ala Met Giu Ile ctc gcc Leu Ala gaa gca ggc Glu Ala Gly gat gaa gca Asp Glu Ala tac Tyr gca ggc cgr gga Ala Gly Arg Gly tcc aaa tcc ctc Ser Lys Ser Leu aac gtt ttr Asn Val Phe aaa gat atc Lys Asp Ile aac aac gtt gaa Asn Asn Val Glu rtc ttc ctt cgc Phe Phe Leu Arg gcc arc Ala Ilie tac gtt gct ggt Tyr Val.Ala Gly cag ctc gat ttg Gin Leu Asp Leu ggt Gly arc acc ggc cgc Ile Thr Gly Arg ctt gct cgc gat Leu Ala Arg Asp rcc Ser cag gct gat gtc Gin Ala Asp Val cac His 80 gaa gtt ctt tcc Giu Val Leu Ser ctc Leu ggc ttc ggt tcc Gly Phe Gly Ser act ttc cgt tac Thr Phe Arg Tyr gca cca gct gat Ala Pro Ala Asp gaa gag Giu Glu 100 rgg agc arc Trp Ser Ilie aac ctr gtt Asn Leu Val 120 gaa Glu 105 aag ctc gac ggc Lys Leu Asp Gly cgc atc gct acc Arg Ile Ala Thr tct tac ccc Ser Tyr Pro 115 cgc gat gac ctc Arg Asp Asp Leu gca Aia 125 gca cgt ggg ctt tcc gct gag gtg Ala Arg Gly Leu Ser Ala Giu Val 130 crc cgc Leu Arg 135 crc gac ggt gca Leu Asp Gly Ala gra Val1 140 gag gra tcc arc Giu Val Ser Ile aag Lys 145 ctt ggt gtc gca Leu Giy Val Ala WO 01/00843 WO 010084PCT/IBOO/00923 gat Asp 150 ggt Gly gtt Val1 cgc Arg tac Tyr ggc G ly 230 gct Ala aag Lys gcc atc gcc gat gtt gta Ala Ile Ala Asp Val Val tcc acc ggc cgc acg ctg cgt Ser Thr Gly Arg Thr Leu Arg ctt gca cct ttC Leu Ala Pro Phe 170 ggc cgc aag gat Gly Arg Lys Asp 185 cgc atc cag gga Arg Ilie Gin Gly 200 aac gtc gac cgc Asn Val Asp Arg 215 tta tcc ggc cca Leu Ser Gly Pro gta cgc gcc atg Val Arg Ala Met 250 ctt gct gga ctc gag gtt ctg Giu Val Leu aag gtc acc Lys Vai Thr 190 ttg cac gcg Leu His Ala 205 aac ctg gac Asn Leu Asp 220 gta tcc cca Val Ser Pro cca cgc agg Pro Arg Arg gct gaa gcc tct Ser cag Gin t tC Phe act Thr 225 cgc Arg aac Asn gct Ala cag caa Gin Gin 165 gtc att Vai Ile 180 ctg ctt Leu Leu ctg gat Leu Asp acc cca Thr Pro tgg gtt Trp Val 245 atg gat Met Asp 260 atc cgc Ile Arg Leu Ala Giy-Leu Gly Ala Giu Ala Ile Leu 265 270 atc tagttttaac tacccccgaa aat Ile atc gcc cgc Ile Ala Arg 280 <210> 376 <211> 281 <212> PRT <213> Corynebacteriun giutamicun <400> 376 Met Leu Lys Ilie Ala Val Pro Asn I 1 Met Giu Ilie Leu Ala Giu Ala Gly Ser Leu Asn Val Phe Asp Giu Alaj Arg Pro Lys Asp Ile Ala Ile Tyr 55 Gly Ilie Thr Gly Arg Asp Leu Ala 70 Giu Val Leu Ser Leu Gly Phe Gly Ser Gly Val Gly Ser Thr Leu Arg Giu Gly Gin Phe WO 01/00843 WO 0100843PCT1IIBO0I00923 Pro Ala Asp Glu Glu Trp, Ser Ile Giu Lys Leu Asp Gly Lys Arg Ile 100 105 110 Ala Thr Ser Tyr Pro Asn Leu Val Arg Asp Asp Leu Ala Ala Arg Gly 115 120 125 Leu Ser Ala Giu Val Leu Arg Leu Asp Gly Ala Val Glu Val Ser Ile 130 135 140 Lys Leu Gly Val Ala Asp Ala Ile Ala Asp Val Vai Ser Thr Gly Arg 145 150 155 160 Thr Leu Arg Gin Gin Giy Leu Ala Pro Phe Gly Glu Val Leu Cys Thr 165 170 175 Ser Giu Ala Val Ile Vai Giy Arg Lys Asp Giu Lys Val Thr Pro Giu 180 185 190 Gin Gin Ile Leu Leu Arg Arg Ile Gin Gly Ile Leu His Ala Gin Asn 195 200 205 Phe Leu Met Leu Asp Tyr Asn Val Asp Arg Asp Asn Leu Asp Ala Ala 210 215 220 Thr Ala Val Thr Pro Gly Leu Ser Gly Pro Thr Val Ser Pro Leu Ala 225 230 235 240 Arg Asp Asn Trp Val Ala Val Arg Ala Met Val Pro Arg Arg Ser Ala 245 250 255 Asn Ala Ilie Met Asp Lys Leu Ala Gly Leu Gly Ala Giu Ala Ile Leu 260 265 270 Ala Ser Glu Ilie Arg Ile Ala Arg Ile 275 280 <210> 377 <211> 393 <212> DNA <213> Corynebacterium giutamicum <22 0> <221> CDS <222> (101)..(370) <223> RXA02195 <400> 377 tcgagccggc aggtgtcgca aaataaaacc aggtggggga gtgaaattat tcgactaata tcctccccca aacacacatt gataactgtt gtgtggaaga atg tac cga gtg aag 115 Met Tyr Arg Val Lys 1 aca ttt gac tcg ctg tac gaa gaa ctt ctt aac cgt gct cag acc cgc 163 Thr Phe Asp Ser Leu Tyr Glu Giu Leu Leu Asn Arg Ala Gin Thr Arg 15 cct gaa ggg tct gga acc gtg gcc gcc ttg gat aaa ggc atc cat cat 211 Pro Giu Gly Ser Gly Thr Val Ala Ala Leu Asp Lys Gly Ile His His WO 01/00843 WO 0100843PCT/iBOO/00923 cta ggt Leu Gly gag tat Giu Tyr tat tgg Tyr Trp aag Lys gag Glu acc Thr atc gaa gaa gcc gga gag gtc tgg att gca gcc Ilie Glu Giu Ala Gly Giu Val Trp Ile Ala Ala 45 gaa gag cta gcc gga gaa atc tcc cag ctc att Glu Giu Leu Ala Gly Glu Ile Ser Gln Leu Ile 60 atc atg gtt gct cgc ggc ctg aag cca gaa gat Ile Met Val Ala Arg Giy Leu Lys Pro Glu Asp 75 80 taggagtttt aaagcaatca tgt 259 307 355 393 atc Ilie tac aag aac Tyr Lys Asn <210> 378 <211> <212> PRT <213> Corynebacteriun giutamicum <400> 378 Met Tyr Arg Val Lys Thr Phe Asp Ser 1 5 Arg Ala Gin Thr Arg Pro Glu Giy Ser 25 Lys Giy Ilie His His Leu Giy Lys Lys 40 Val Trp Ilie Ala Ala Glu Tyr Glu Thr 55 Ilie Ser Gin Leu Ile Tyr Trp, Thr Gin 70 Leu Lys Pro Giu Asp Ilie Tyr Lys Asn Tyr Giu Giu Leu Leu Thr Val Ala Ala Leu Ilie Giu Glu Ala Gly Giu Glu Leu Aia Gly Ile Met Val Ala Arg <210> 379 <211> 477 <212> DNA <213> Corynebacterium giutamicum <2 <221> CDS <222> (101)..(454) <223> RXA01097 <400> 379 gcgccaacgc agtgcttgcc gcgaccattt tccacttccg Cgaagtaacc atcgccgaag taaagggagc cattaaagat gcaggatttg aggtgcggaa atg agt gac aat cca 115 Met Ser Asp Asn Pro 1 WO 01/00843 caa gag ti Gin Giu T) gac Asp gtc Val gcg Ala atc Ile ctc Leu ggt Gly ctg Lgu gcc ggc ctg gCg Aia Giy Leu Val ctc atg atg gcc Leu Met Met Ala acc cgc cgt gga Thr Arg Arg Giy 'aag ggc ctg acc Lys Giy Leu Thr gac tgc gac ggc Asp Cys Asp Giy gcg tgc cac act Aia Cys His Thr 105 ga t Asp ccg Pro tgg Trp acc Thr tc t Ser gac Asp ggt Gly cga tta Arg Leu gac ggg Asp Giy cta gcc Leu Ala cgc aac Arg Asn gtc acc Vai Thr gtg aaa Val Lys gac aat aag Lys acc Thr tat Tyr PCT/iBOOIOO923 ctt aac 163 Leu Asn aac gag 211 Asn Giu act ttg 259 Thr Leu gag tac Giu Tyr gga ctt Giy Leu caa acc Gin Thr 100 gac gtt gcc cac aca Ala His Thr Cys Phe Asp Asn Asp Val Leu taaaagcaac aacgattaag gaa <210> 380 <211> 118 <212> PRT <213> Corynebacterium giutanicun <400> 380 Met Ser Asp Asn Pro Gin Giu Tyr Giu 1 5 Arg Leu Lys Leu Asn Asp Ala Giy Leu Asp Gly Thr Asn Giu Val Leu Met Met Leu Ala Tyr Thr Leu Aia Thr Arg Arg Arg Asn Giu Tyr Trp Ile Lys Giy Leu 70 Val Thr Gly Leu Ala Leu Asp Cys Asp Val Lys Gin Thr Gly Giy Ala Cys His 100 105 Asp Asn Asp Val Leu Leu Glu Gin His Arg Gin Leu Cys Lys Ala Ala Ser Glu Thr Phe WO 01/00843 WO 0100843PCTAJBOOIOO923 <210> <211> <212> <213> <220> <221> <222> <223> 381 861

DNA

Corynebac terium g lu tamicum

CDS

(101)..(838) RXA01100 <400> 381 gctgtgggct. actcaattcc acccagaaaa atcaggtgac gcaggcgcac agctactgcg aaactggatc aactacatct aacagatagg atcaatattc atg acc ttc act att Met Thr Phe Thr Ile ctt cct gca gtc Leu Pro Ala Val gat Asp gta gtt aac gga caa gca gtt cgc cta Val Val Asn Gly Gin Ala Val Arg Leu 15 gat cag Asp Gin ggc gag gcc Gly Giu Ala ctg aag tgg Leu Lys Trp, act gaa aag tct Thr Glu Lays Ser ggc acc cct ttg Gly Thr Pro Leu gaa tcc gca Giu Ser Ala gtg gac ctg Val Asp Leu cag gag cag ggt Gin Glu Gin Gly aag tgg ttg cac Lys Trp Leu His ttt Phe gac gca gcg ttc aac cgt Asp Ala Ala Phe Asn Arg tcc aac cat gag atg atg gcg gaa att Ser Asn His Glu Met Met Ala Glu Ile gtc Val ggc aag ctc gat Gly Lys Leu Asp gtt Val gat gtg gag ctc Asp Val Giu Leu ggc ggt atc cgt Gly Gly Ile Arg gat gag tct ctg Asp Glu Ser Leu cgc gcg ctg gca Arg Ala Leu Ala ggt gca cgt cgt Gly Ala Arg Arg gta aac Val Asn 100 att. ggt acc Ile Gly Thr caa cgc tat Gin Arg Tyr 120 gct ctg gag aag cca gag tgg att gct tct gcg att Ala Leu Glu Lys Pro Glu Trp Ile Ala Ser Ala Ile 110 115 ggc gag aag att Gly Glu Lys Ilie gtc gat atc gct.

Val Asp Ile Ala cgt ttg gaa.

Arg Leu Glu gat ggt Asp Gly 135 gaa tgg cgc acc Glu Trp Arg Thr cgt Arg 140 gga aac ggt tgg Gly Asn Gly Trp gtc Val1 145 tcc gat ggt ggc Ser Asp Gly Gly 499 547 595 gat Asp 150 ctg tgg gaa gtt Leu Trp Giu Val c tc Leu 155 gag cgt ttg gat Glu Arg Leu Asp tcc Ser 160 caa ggt tgt gca Gin Gly Cys Ala cgt Arg 165 ttc gtg gtt acc gat gtg tcc aag gac ggc acc ttg agt ggt cca aat WO 01/00843 Phe Val Val Thr Asp Val Ser Lys Asp 170 gtt gag ctg ctg cgt gag gtt gct gca Val Giu Leu Leu Arg Giu Val Ala Ala 185 190 gca tct ggt gga att tct gtt ttg gaa Ala Ser Gly Gly Ilie Ser Val Leu Giu 200 205 tac cag gat gag ggc att gat tcc gtc Tyr Gin Asp Giu Gly Ile Asp Ser Val 215 220 gag cac aag ttc acc ctc gaa gag gct Giu His Lys Phe Thr Leu Giu Glu Ala 230 235 ggt taatacatgg atgctcgtgg gat Gly <210> 382 <211> 246 <212> PRT <213> Corynebacteriun giutarnicum <400> 382 Met Thr Phe Thr Ilie Leu Pro Ala Val Gly 175 gct Ala gat Asp atc Ile ttg Leu Leu gac Asp t tg Leu ggc Gly 225 gca Ala Ser gca Ala gaa Giu 210 aag Lys G ly cct Pro 195 c ta Leu gca Ala PCTIIBOO/00923 -o Asn :c gtg 691 Le Val gta gaa aag Val Glu Lys 1 Val Pro His Met Giy Ala Ile Ala Val1 145 Gin Arg Leu Phe Met Gly Arg Ala Val 130 Ser Gly Leu Glu Val Ala Ile Arg Ser 115 Arg Asp Cys Asp Ser Asp Glu Arg Val1 100 Ala Leu Gly Ala Giy Leu Asp Val 70 Asp Ile Gin Asp Asp 150 Phe Giu Lys Ala 55 Gly Glu Gly Arg Gly 135 Leu Val1 Ala Trp Ala Lys Ser Thr Tyr 120 Glu Trp Val Gly Gin Phe Leu Leu Ala 105 Gly Trp Giu Thr Asp Val 10 Thr Giu Glu Gin Asn Arg Asp Val 75 Giu Arg Ala Leu Giu Lys Arg Thr Val Leu 155 Asp Val Val1 Lys Gly Gly Asp Ala Giu Ile Arg 140 Giu Ser Gin Gly Trp His Leu Thr Giu Asp Gly Asp Gly Ala Thr Leu Glu Thr Gly Trp Ile Trp Ser 160 Thr WO 01/00843 Leu Asp Leu Gly 225 Ala PCTIiBOO/00923 170 175 Val Glu Leu Leu Arg Glu Val Ala Ala Ala Thr 185 190 Ala Ser Gly Gly Ile Ser Val Leu Giu Asp Val 200 205 Tyr Gin Asp Giu Gly Ile Asp Ser Val Ile Ile 215 220 Glu His Lys Phe Thr Leu Giu Glu Ala Leu Ala 230 235 240 Gly <210> 383 <211> 756 <212> DNA <213> Corynebacterium giutaiicun <22 0> <221> CDS <222> (101)..(733) <223> RXA01101 <400> 383 atcgcagcta ggccagtgtg gtggatataa aacccttttg gggagaaaga aactcgactg cggttcttga tcctgaaagc acgtgacata aactatcggc atg acc aaa act gte Met Thr Lys Thr Val gce Ala cta Leu tgc Cys gcc Ala cag Gin cag Gin tgc Cys gac Asp gcc Ala gc t Ala aag Lys gct Ala t te Phe tgg Trp tac Tyr ggt Gly gat Asp ggt Gly ggt Gly gat Asp cc t Pro gga Gly gca Ala ggc Gly t tg Leu gga Gly 75 gaa Giu ggc Gly tct Ser gaa Giu etc Leu aaa Lys cgt Arg ggc Gly aaa Lys gga Gly gtt Val eta Leu aac Asn c ca Pro gat Asp gtg Val aac Asn ate Ile 30 gt t Val1 gtC Val1 gtg Val1 gag Glu gaa Giu eaa Gin eea Pro 35 gea Ala att Ile gtg Val1 tea Ser ege Arg 20 gaa Giu ttt Phe ate Ile gge Gly get Ala 100 gea Ala gtt Val1 gat Asp gga Gly atg Met ggt Gly ege etc eaa geg gag ate etg Arg Leu Gin Ala Giu Ile Leu WO 01/00843 WO 0100843PCT/IBOO/00923 105 110 115 cct cac atg ggg tgg aac aca ctt gaa atg cct acc aac tca cca atg 499 Pro His Met Gly Trp Asn Thr Leu Glu Met Pro Thr Asn Ser Pro Met 120 125 130 ttt gag gga att tca cct gat gag cgt ttc tac ttc gtg cac tcc tat 547 Phe Giu Gly Ile Ser Pro Asp Giu Arg Phe Tyr Phe Val His Ser Tyr 135 140 145 ggt gtg cgc aag tgg acg ttg gaa acc gac gat ctg acc acg cct cca 595 Gly Val Arg Lys Trp Thr Leu Glu Thr Asp Asp Leu Thr Thr Pro Pro 150 155 160 165 gag gtt gtg tgg gcg aag cac gaa aat gat cgt ttt gtg gca gct gtg 643 Giu Val Val Trp Ala Lys His Glu Asn Asp Arg Phe Val Ala Ala Val 170 175 180 gaa aac ggc acg ctg tgg gct act caa ttc cac cca gaa aaa tca ggt 691 Glu Asn Gly Thr Leu Trp, Ala Thr Gin Phe His Pro Glu Lys Ser Gly 185 190 195 gac gca ggc gca cag cta ctg cga aac tgg atc aac tac atc 733 Asp Ala Gly Ala Gin Leu Leu Arg Asn Trp Ile Asn Tyr Ile 200 205 210 taacagatag gatcaatatt cat 756 <210> 384 <211> 211 <212> PRT <213> Corynebacterium glutamicum <400> 384 Met Thr Lys Thr Val Ala Leu Leu Asp Tyr Gly Ser Giy Asn Leu Arg 1 5 10 Ser Ala Gin Arg Ala Leu Glu Arg Ala Gly Ala Giu Val Ile Val Ser 25 Ser Asp Pro Glu Val Cys Thr Asn Ala Asp Gly Leu Leu Val Pro Gly 40 Val Gly Ala Phe Asp Ala Cys Met Lys Gly Leu Lys Asn Val Phe Gly 55 His Arg Ilie Ilie Gly Gin Arg Leu Ala Gly Gly Arg Pro Val Met Gly 70 75 Ilie Cys Val Gly Met Gin Ile Leu Phe Asp Glu Gly Asp Glu His Gly 90 Ile Lys Ser Ala Gly Cys Gly Giu Trp Pro Gly Lys Val Giu Arg Leu 100 105 110 Gin Ala Giu Ilie Leu Pro His Met Gly Trp Asn Thr Leu Glu Met Pro 115 120 125 Thr Asn Ser Pro Met Phe Glu Gly Ile Ser Pro Asp Giu Arg Phe Tyr 130 135 140 WO 01/00843 WO 0100843PCTAlBOO00923 Phe Val His Ser Tyr Gly Val Arg Lys Trp Thr Leu Glu Thr Asp Asp 145 150 155 160 Leu Thr Thr Pro Pro Glu Val Val Trp Ala Lys His Giu Asn Asp Arg 165 170 175 Phe Vai Ala Ala Val Glu Asn Giy Thr Leu Trp Ala Thr Gln Phe His 180 185 190 Pro Glu Lys Ser Gly Asp Ala Gly Ala Gin Leu Leu Arg Asn Trp Ile 195 200 205 Asn Tyr Ile 210 <210> 385 <211> 723 <212> DNA <213> Corynebacterium giutamicun <22 0> <221> CDS <222> (700) <223> RXN01657 <400> 385 cctccgtcat tgccgacgta tcccgcggcc tgggtgaagc catggtgggc atcaacgtat ccgacgttcc agcaccacac cgactcgccg agcgcggctg gtg atc gtt gga gtt Val Ile Val Gly Val t ta Leu ctc Leu ctc Leu ctg Leu gac Asp aaa Lys gac Asp ggC Gly ac c Thr gtc Val1 ttc Phe gtt Val1 aac Asn cgt Arg ggg Giy cga Arg atc Ilie gac Asp ttc Phe 75 cca Pro cga Arg gaa cac ctc Glu His Leu cgt gtg cca Arg Val Pro ggg gaa tcc Gly Glu Ser gaa cct cta Glu Pro Leu tgc gct ggc Cys Ala Gly gga caa caa Giy Gin Gin ttt ggc gcc Phe Giy Ala 110 gcc Ala gac Asp gtg Val1 aat Asn atc Ile ttg Leu ttg Leu ctt Leu ttg Leu ctc Leu tat Tyr gcg Ala 115 163 211 259 307 355 403 451 caa cgc gaa tcc ttc Gin Arg Giu Ser Phe 115 WO 01/00843 WO 0100843PCTIIBOO/00923 gac acc acc gtg gat gtt tcc ttc gac ggt gca aca ttc ccc gga gtg 499 Asp Thr Thr Val Asp Val Ser Phe Asp Gly Ala Thr Phe Pro Gly Val 120 125 130 cag gcc tcg ttt atc cga gct ccc atc gtc act gct ttt ggt cct acg 547 Gin Ala Ser Phe Ilie Arg Ala Pro Ile Val Thr Ala Phe Gly Pro Thr 135 140 145 gta gaa gcg atc gct gct ctc aac ggt ggg gag gtg gtt ggt gta cgc 595 Val Giu Ala Ile Ala Ala Leu Asn Gly Gly Glu Vai Val Gly Val Arg 150 155 160 165 caa ggc aac atc atc gcg ctg tct ttc cat ccc gaa gaa acc ggc gat 643 Gin Gly Asn Ilie Ilie Ala Leu Ser Phe His Pro Giu Giu Thr Gly Asp 170 175 180 tac cgc atc cac caa gcc tgg ctg gac ctg gtg aga aaa cac gct gaa 691 Tyr Arg Ilie His Gin Ala Trp Leu Asp Leu Vai Arg Lys His Ala Giu 185 190 195 ctg gcg att tgatgttttc ggtagcgctc tgt 723 Leu Ala Ile 200 <210> 386 <211> 200 <212> PRT <213> Corynebacteriun giutamicun <400> 386 Vai Ilie Val Gly Val Leu Ala Leu Gin Giy Gly Val Giu Giu His Leu 1 5 10 Thr Ala Leu Giu Ala Leu Gly Ala Thr Thr Arg Lys Val Arg Val Pro 25 Lys Asp Leu Asp Gly Leu Giu Gly Ile Val Ile Pro Gly Gly Giu Ser 40 Thr Val Leu Asp Lys Leu Ala Arg Thr Phe Asp Val Val Giu Pro Leu 55 Ala Asn Leu Ilie Arg Asp Giy Leu Pro Val Phe Ala Thr Cys Ala Gly 70 75 Leu Ilie Tyr Leu Ala Lys His Leu Asp Asn Pro Ala Arg Gly Gin Gin 90 Thr Leu Ala Val Vai Asp Vai Val Val Arg Arg Asn Ala Phe Gly Ala 100 105 110 Gin Arg Giu Ser Phe Asp Thr Thr Val Asp Val Ser Phe Asp Gly Ala 115 120 125 Thr Phe Pro Gly Val Gin Ala Ser Phe Ilie Arg Ala Pro Ilie Vai Thr 130 135 140 Ala Phe Giy Pro Thr Val Giu Ala Ile Ala Ala Leu Asn Gly Gly Giu 145 150 155 160 WO 01/00843 WO 0100843PCTIBOOIOO923 Val Val Gly Val Arg Gin Gly Asn Ile Ile Ala Leu Ser Phe His Pro 165 170 175 Giu Glu Thr Gly Asp Tyr Arg Ile His Gin Ala Trp Leu Asp Leu Val 180 185 190 Arg Lys His Ala Giu Leu Ala Ile 195 200 <210> 387 <211> 601 <212> DNA <213> Corynebacteriun glutamicum <22 0> <221> CDS <222> (101)..(601) <223> FRXA01657 <400> 387 cctccgtcat tgccgacgta tcccgcggcc tgggtgaagc ccgacgttcc agcaccacac cgactcgccg agcgcggctg catggtgggc atcaacgtat gtg atc gtt gga gtt 11 Val Ile Val Gly Val tta Leu ctc Leu ctc Leu ctg Leu gac Asp aaa Lys gac Asp gac Asp ctc Leu 9c9 Ala ggc Gly cgg Arg cta Leu C tC Leu gtg Val acc Thr 120 cag Gin acg Thr atc Ile aca Thr ccc Pro gac Asp gtg Val1 105 gtg Val1 ctc Leu c ca Pro tcc Ser cta Leu ggc Gly caa Gin gcc Ala gca Ala 1 acc Thr aag Lys acc Thr gcg Ala ctg Leu ac c Thr caa Gin aca Thr gcc Ala gac Asp gtg Val aat Asn atc Ile ttg Leu cgc Arg t tc Phe 130 t tg Leu ctt Leu t tg Leu ctc Leu tat Tyr gcg Ala gaa Giu 115

CCC

Pro cag gcc tcg ttt atc cga gct ccc atc gtc act gct ttt 99t cct acg WO 01/00843 PCTIIBOO/00923 Gin Ala Ser Phe Ilie Arg Ala Pro Ile Val Thr Ala Phe Gly Pro Thr 135 140 145 gta gaa gcg atc gct gct ctc aac ggt ggg gag gtg gtt ggt gta cgc 595 Val Glu Ala Ile Ala Ala Leu Asn Gly Gly Giu Val Val Gly Val Arg 150 155 160 165 caa ggc 601 Gin Gly <210> 388 <211> 167 <212> PRT <213> Corynebacterium glutamicun <400> 388 Val Ile Val Gly Val Leu Ala Leu Gin 1 Thr Lys Thr Ala Leu Thr Gin Thr Ala 145 Val Leu Leu Leu Asp 70 Lys Asp Asp Gin Val 150 Gin Gly Giu Ala Gly His Val Thr Ala 135 Giu Gly G ly 10 Thr Val1 Phe Val1 Asn Arg Asp Ile Ala Giu Val1 Gly Val1 Thr Arg Ala Phe 125 Pro Asn Giu Arg Gly Giu Cys Gly Phe 110 Asp Ile Gly His Val Giu Pro Ala Gin Gly Gly Val Gly Leu Pro Ser Leu Gly Gin Ala Ala Thr Giu 160 <210> 389 <211> 897 <212> DNA <213> Corynebacterium giutanicum <22 0> <221> CDS <222> (101)..(874) <223> .RXA01098 WO 01/00843 WO 0100843PCTIiBOO/00923 <400> 389 aaaagggctc acgatgtgct gttaagtaag attgaaaaag ttcggttgat gcatgcagat gcaggtaatg accagtcgtt aaatgaggag tacaagtaaa atg ggc gtg gca att Met Gly Val Ala Ile cga Arg gtg Val1 aag Lys acc Thr ac c Thr agc Ser tcg Ser tcc Ser cgc Arg ac c Thr 150 aag Lys gac Asp gtt Val1 gtt Val1 aac Asn cgc Arg gcc Ala gc t Ala gaa Giu g tg Val1 aag Lys gtt Val1 135 cac His cgc Arg ggc Gly cgc Arg gac aac Asp Asn gct ggc Ala Gly gat gag Asp Glu acc atg Thr Met ctg act Leu Thr ctg cgc Leu Arg cgt cca Arg Pro 110 atc gtg Ile Val cag cca Gin Pro gaa ctt Giu Leu ggc gaa Gly Giu 175 ttt gac Phe Asp 190 gta atc Val Ilie gtt Val gtg Val1 ttc Phe gtt Val1 ggC Gly gcc Ala ctg Leu gtg Val1 130 ttt Phe atc Ile c tc Leu ctg Leu ggc Gly 210 gtt Val1 gag Giu Ctg Leu gtt Val1 ggC Gly gac Asp tca Ser 115 gat Asp gaa Giu gag Glu aac Asn ctg Leu 195 ggc Gly aag gcg gag cat ttc cca cca gct gtt gca gct ggc gcc aac gca gtg WO 01/00843 PCT/LBOO/00923 Lys Ala Glu His Phe Pro Pro Ala Val Ala Ala Gly Ala Asn Ala Val 215 220 225 ctt gcc gcg acc att ttc cac ttc cgc gaa gta acc atc gcc gaa gta 835 Leu Ala Ala Thr Ile Phe His Phe Arg Glu Val Thr Ile Ala Glu Val 230 235 240 245 aag gga gcc att aaa gat gca gga ttt gag gtg cgg aaa tgagtgacaa 884 Lys Gly Ala Ile Lys Asp Ala Gly Phe Glu Val Arg Lys 250 255 tccacaagag tat 897 <210> 390 <211> 258 <212> PRT <213> Corynebacteriun glutamicun <400> 390 Met Gly Val Ala Ile Arg Val Ile Pro Cys Leu Asp Val Asp Asn Gly 1 Arg Pro Thr Asp Gly Gly Leu Ser Gly 145 Ala Leu Glu Val1 Lys Thr Thr Ser Ser Ser Arg Thr 150 Lys Asp Val1 10 Phe Glu Asn Tyr Asp Glu Ser Lys His Asp Gin Val Glu Asp Val Asn Thr Ser 105 Arg Phe Gly 120 Pro Giu Giy Gly Gly Ser Gly Glu Glu 170 Asp Gly Thr 185 Ala Ala Val 200 Glu His Phe Ala Asp Thr Leu Leu Arg 110 I le Gin Giu Gly Phe 190 Val1 Val1 Gly Glu Met Thr Arg Pro Val1 Pro Leu Glu 175 Asp Ile Ala Ser Giy 210 Gly Ala Gly Lys WO 01/00843 WO 0100843PCTIBOO/00923 Gly Ala Asn Ala Val Leu Ala Ala Thr Ile Phe His Phe Arg Glu Val 225 230 235 240 Thr Ile Ala Glu Val Lys Gly Ala Ile Lys Asp Ala Gly Phe Glu Val 245 250 255 Arg Lys <210> 391 <211> 729 <212> DNA <213> Corynebacterium glutanicum <220> <221> CDS <222> (101)..(706) <223> RXN01104 <400> 391 cacttgcgca ctaccattgg tgtgcctgag gaaaatgatg cgtttttgga cgcagctgca gagatcatca agctgaacct gtaagagaga agaatttttc atg act gtc gca cca Met Thr Val Ala Pro aga Arg gag Glu c ca Pro gat Asp acc Thr att Ile atg Met tac ggt ac c Thr ctg Leu gac Asp gtc Val1 gat Asp aag Lys gca Ala 105 a tc Ile tac gca Ala gac Asp cac His cat His acc Thr aaa Lys tta Leu tcC Ser gca acc Thr aaa Lys 30 gca Ala gac Asp ctc Leu cg t Arg g tg Val 110 gac Asp aac agc Ser 15 gta Val1 ttc Phe atc Ile ggc Gly t tc Phe 95 gtg Val c ac His gag gac Asp gat Asp cac His gac Asp ctc Leu tgc Cys tcc Ser acc Thr 130 ttt atc Ile acc Thr ggC G ly gca Ala ctt Leu cag Gin ggt G ly 115 tcC Ser gaa acc Thr ggc Gly agt Ser cac His gac Asp ctg Leu 100 cgc Arg g tg Val1 ac c 115 163 211 259 307 355 403 451 499 547 WO 01/00843 Gly Gly His Tyr 135 gcg ctc aac tcc Ala Leu Asn Ser 150 gac cct cac cac Asp Pro His His ctg cgc ggt gcc Leu Arg Gly Ala 185 act aag gga gcg Thr Lys Gly Ala 200 Thr Val Ile Asn Giu His P1 140 1 atc acc ctc cac gtg atc t( Ile Thr Leu His Val Ile C) 155 160 acc gaa gca gag tac aag g( Thr Glu Ala Giu Tyr Lys A] 175 gag atg gat cct cgt caa a( Giu Met Asp Pro Arg Gin T1 190 tagacatgaa ctcttctccc atc PCTIIBOO/00923 Thr Leu ggc cgc 595 Gly Arg 165 cgt gcg 643 Arg Ala 180 cca tcc 691 Pro Ser <210> 392 <211> 202 <212> PRT <c213> Corynebacterium giutamicun <400> 392 Met Thr Val Ala Pro Arg Ilie Gly Thr 1 Ser Ile Val1 Ile Ala Ser Ile Ile Phe 145 Cys Ala Asp Asp His Asp Leu Cys Ser Thr 130 Phe His Val Thr Gly Ser His Asp Leu 100 Arg Val Thr Gly Arg 180 Ile Phe Leu 55 Val1 Giy Asp Phe Gly 135 Leu Pro Arg Asn Phe Lys Glu Asp Giu Val1 120 His Asn His Gly Leu 25 Asp Val Asp Lys Ala 105 Ile Tyr Ser His Ala 185 Ala 10 Asp His His Thr Lys Leu Ser Ala Arg Ile 170 Thr Gly Met Ala Ala 75 Gly Val1 Gly Thr Ile 155 Thr Arg Thx- Leu Lys Ile Ile Giu Giu Val 140 Thr Giu Thr Gly Thr Gly Val1 Arg S er Pro 125 Ile Leu Ala Thr Lys Ala Asp Leu Arg Val1 110 Asp Asn His Glu Ser Val Phe Ile Giy Phe Val1 His Giu Val Tyr 175 Giu Asp Gly Glu Gin Ala Asp Met His Ile 160 Lys Val Giu Met Asp Pro Arg Gin 190 WO 01/00843 WO 0100843PCTIIBOO/00923 Thr Gly Ile Pro Ser Thr Lys Gly Ala Leu 195 200 <210> <211> <212> <213> <220> <221> <222> <223> 393 729

DNA

Corynebacteriun glutamicum

CDS

(101)..(706) FRXA0 1104 <400> 393 cacttgcgca ctaccattgg tgtgcctgag gaaaatgatg cgtttttgga cgcagctgca gagatcatca agctgaacct gtaagagaga agaatttttc atg act gtc gca cca Met Thr Val Ala Pro aga att ggt acc Arg Ile Gly Thr acc cgc acc acc agc gaa tcc gac atc Thr Arg Thr Thr Ser Glu Ser Asp Ile acc gtc Thr Val gag atc aac Glu Ile Asn cca ttt ttc Pro Phe Phe gac ggc acc ggc Asp Gly Thr Gly gta gat atc gat Val Asp Ile Asp acc ggc ctg Thr Gly Leu ggc agt ttt Gly Ser Phe gac cac atg ctc act gca ttc ggc gtg Asp His Met Leu Thr Ala Phe Gly Val c ac His gat ctg Asp Leu aaa gtc cat gcc Lys Val His Ala ggc gac atc gag Gly Asp Ile Glu gac-gca cac cac Asp Ala His His ac c Thr gtg gaa gat acc Val Glu Asp Thr atc gtg ctc ggc Ilie Val Leu Gly gca ctc ctt gac Ala Leu Leu Asp att ggc gac aag Ilie Gly Asp Lys aaa Lys ggc atc cgc cgt Gly Ile Arg Arg gca tcc tgc cag Ala Ser Cys Gin ctg ccc Leu Pro 100 atg gat gag Met Asp Glu tac ttc gtg Tyr Phe Val 120 tta gtg gag tcc gtg gtg gat atc tcc Leu Val Glu Ser Val Val Asp Ile Ser ggt cgc cca Gly Arg Pro 115 tcc gtg atc Ser Val Ile atc tcc ggc gaa cca gac cac atg atc Ile Ser Gly Giu Pro Asp His Met Ile 125 acc Thr 130 ggt gga Gly Gly 135 cac tac gca acc gtg atc aac gag cac His Tyr Ala Thr Val Ile Asn Glu His ttt gaa acc ctc Phe Giu Thr Leu gcg Ala 150 ctc aac tcc cga Leu Asn Ser Arg acc ctc cac gtg atc tgc cac tac ggc Thr Leu His Val Ile Cys His Tyr Gly 160 WO 01/00843 gac cct cac Asp Pro His ctg cgc ggt Leu Arg Gly act aag gga Thr Lys Gly 200 PCT/EBOO/00923 cac atc His Ile 170 gcc gta Ala Val 185 gcg ctc Ala Leu acc gaa gca gag tac aag gct gtt gcc cgt gcg 643 Thr Glu Ala Glu Tyr Lys Ala Val Ala Arg Ala 175 180 gag atg gat cct cgt caa aca gga atc cca tcc 691 Glu Met Asp Pro Arg Gin Thr Gly Ile Pro Ser 190 195 tagacatgaa ctcttctccc atc 729 <210> 394 <211> 202 <212> PRT <213> Corynebacterium glutamicum <400> 394 Met Thr Val Ala Pro Arg Ile Gly Thr 1 Ser Ile Val Ile Ala Ser Ile Ile Phe 145 Cys Ala Thr Asp Asp His Asp Leu Cys Ser Thr 130 Phe His Val Gly Ile Thr Gly Ala Leu Gin Gly 115 Ser Giu Tyr Ala Ile 195 Thr Gly Ser His Asp Leu 100 Arg Val1 Thr Gly Arg 180 Pro 5 Val1 Leu Phe His Ala Pro Pro Ile Leu Arg 165 Ala Ser Glu Pro Asp Thr 70 Ile Met Tyr Gly Ala 150 Asp Leu Thr Ile Phe Leu 55 Val Gly Asp Phe Gly 135 Leu Pro Arg Lys Asn Phe 40 Lys Giu Asp Glu Val1 120 His Asn His Gly Gly 200 Ala Asp His His Thr Lys Leu Ser Ala Arg Ile 170 Val1 Leu Thr Gly Met Ala Ala Gly Val1 Gly Thr Ile 155 Thr G iu Arg Thr Leu Lys Ilie Ile Giu Giu Val 140 Thr Gi u Met Thr Gly Thr Gly Val Arg Ser Pro 125 Ile Leu Ala Asp Thr Lys Ala Asp Leu Arg Val1 110 Asp Asn His Giu Pro 190 <210> 395 WO 01/00843 WO 0100843PCTJ/IBOO/00923 <211> <212> <213> <220> <221> <222> <223> 987

DNA

Corynebacteriumr glutamicum

CDS

(101)..(964) RXN00446 <400> 395 tgctacgaag ttatctagta atgaagttag tttttcccct ggtgacggag gctacttggg gggctaatcg gtacccggat ctcccggcag cagttgatgc atg ggt gcg gtt Met Gly Ala Val ctc cgt gag gct Leu Arg Glu Ala gca gag cat tta gag gtt gag ttt gac Ala Giu His Leu Glu Val Giu Phe Asp cag gtc Gin Val acg gta ggt Thr Val Gly acg tgc gct Thr Cys Ala tgc Cys ggc tcg tct gcg ctg tgt caa cag ctg Giy Ser Ser Ala Leu Cys Gin Gin Leu 30 gtt cag gca Val Gin Ala agc ttt gag Ser Phe Giu cag ggc gat gag Gin Gly Asp Giu att ttt cca tgg Ile Phe Pro Trp, gct tat Ala Tyr cca att ttc gcg Pro Ile Phe Ala gtc gcg ggc gcc Val Ala Gly Ala act Thr cct gtt gcc att Pro Val Ala Ile ctg act gct gat Leu Thr Ala Asp aat cat gat ctt Asn His Asp Leu gcg atg gca gcc Ala Met Ala Ala atc act gat aag Ile Thr Asp Lys cgc ctc att ttc Arg Leu Ile Phe tgc aac ccc aac Cys Asn Pro Asn aat cct Asn Pro 100 tcg ggc acc Ser Gly Thr gtt cca aac Val Pro Asn 120 acc Thr 105 atc acc cag gcg Ile Thr Gin Ala ttt gat aat ttc Phe Asp Asn Phe atg gaa aag Met Giu Lys 115 ttt gag ttc Phe Giu Phe gat gtc gtt gtt Asp Val Vai Val ctg gat gag gct Leu Asp Giu Ala tat Tyr 130 aac cgc Asn Arg 135 gcg gac gac acc Ala Asp Asp Thr gtt gcc act gag Val Ala Thr Giu gaa Giu 145 atc cac cgc cac Ile His Arg His aac gtg att ggt Asn Val Ile Giy ttg Leu 155 cgc acg ttc tcc Arg Thr Phe Ser aag Lys 160 gcg tat ggc ctg Ala Tyr Giy Leu ggc ttg cgt gtt Gly Leu Arg Val tac gcc ttc gga Tyr Ala Phe Gly gca gag atc atc Ala Glu Ilie Ile gca gcg Ala Ala 180 atg aat aag gtg gct att cct ttc gcg gtg aat tca gca gct cag gcg WO 01/00843 Met Asn Lys Val Ala Ile Pro Phe Ala 185 190 gca gcg ctt gcg agt ttg aat tct gcc Ala Ala Leu Ala Ser Leu Asn Ser Ala 200 205 gag gaa acc gtc gaa aag cgt gat gct Glu Glu Thr Val Glu Lys Arg Asp Ala 215 220 gcg ccg acg cag gcc aat ttc gtc tgg Ala Pro Thr Gin Ala Asn Phe Val Ti-p 230 235 gag-ttg gcg gct aaa ttg gcc gag cac Giu Leu Ala Ala Lys Leu Ala Glu His 250 ccc gag ggt gcg cgc att tcg gtg acc Pro Glu Gly Ala Arg Ile Ser Val Thr 265 270 ctg ctg cgc gcg tgg gag gcc atc aat Leu Leu Arg Ala Trp Glu Ala Ile Asn 280 285 tgc <210> 396 <211> 288 <212> PRT <213> Corynebacterium glutamicum <400> 396 Met Giy Ala Val Glu Leu Arg Giu Ala 1 5 Glu Phe Asp Gin Val Thr Vai Gly Cys Gin Leu Val Gin Ala Thr Cys Ala Gin Trp Arg Ser Phe Giu Ala Tyr Pro Ile Thr Pro Val Ala Ile Pro Leu Thr Ala 70 Ala Met Ala Ala Ala Ile Th- Asp Lys Asn Pro Asn Asn Pro Ser Gly Thr Thr 100 105 Asn Phe Met Giu Lys Val Pro Asn Asp 115 120 Ala Tyr Phe Glu Phe Asn Arg Ala Asp Val1 ga t Asp gtg Val1 ctg Leu ggc Gly 255 aac Asn gc t Ala Asn gag Giu gtg Val ccg Pro 240 a tc Ile gcc Ala ggg Gly PCTIiBOO/00923 Ser Ala Ala Gin Ala 195 ttg atg gaa cgg gtg -739 Leu Met Glu Arg Val 210 tca gcg ctt ggt gct 787 Ser Ala Leu Gly Ala 225 ggc gag ggc gcc gct 835 Gly Glu Gly Ala Ala 245 gtg att cgc gcg ttc 883 Val Ile Arg Ala Phe 260 gag gaa act gac aag 931 Giu Glu Thr Asp Lys 275 tagtctttgg cgttttgcgg 984 Leu Gly Gly Phe Asp Thr Ile Val1

ASP

Ala Ser Asp Ala Gln Arg Thi- Val1 Thi- WO 01/00843 130 Glu Ile His 145 Ala Tyr Gly Glu Ile Ile Ser Ala Ala 195 Leu Met Glu 210 Ser Ala Leu 225 Gly Glu dly Val Ile Arg Glu Glu Thr 275 PCT/BOOIOO923 His Ala 165 Ala Ala Val1 Ala Ala 245 Phe Lys Asp 150 Gly Met Ala Glu Ala 230 Glu Pro Leu Val Arg Lys Leu 200 Thr Thr Ala dly Arg 280

I

I

I le lal lal 185 kla lal ;ln kla kla kla ~tt al1 ~cc 'hr 25 ~tg 4 eu ac yr Ltt Gly Gly 170 Ala Ser Glu Ala Lys 250 Arg Trp gtt Val 10 cc a Pro cgc Arg gcc Ala cc t Leu 155 Tyr Ile Leu Lys Asn 235 Leu Ile Glu ggg Gly gtt Val acg Thr t tc Phe t tc 140 Arg Ala Pro Asn Arg 220 Phe Ala Ser Ala c tg Leu gcc Ala ttc Phe gga Gly gcg Thr Phe Phe Ser 205 Asp Val1 Glu Val Ile 285 gat Asp act Thr tcc Ser aac Asn gtg Phe Gly Ala 190 Ala Ala Trp His Thr 270 Asn gag Glu gag Glu aag Lys gca Ala aat Ser Asn 175 Val1 Asp Val Leu Gly 255 Asn Ala gct Ala gaa Glu gcg Ala gag Glu tca <210> 397 <211> 545 <212> DNA <213> Corynebacteriui glutamicun <2 <221> CDS <222> (522) <223> FRXA00446 <400> 397 atg gaa aag gtt cca aac gat gtc q Met Glu Lys Val Pro Asn Asp Val N 1 5 ttt gag ttc aac cgc gcg gac gac a Phe Glu Phe Asn Arg Ala Asp Asp '1 cac cgc cac gac aac gtg att ggt t His Arg His Asp Asn Val Ile Gly 1 40 ggc ctg gcg ggc ttg cgt gtt ggt t Gly Leu Ala Gly Leu Arg Val Gly 55 atc gca gcg atg aat aag gtg act a tat Tyr a tc Ile tat Tyr atc Ile gca WO 01/00843 Ile Ala Ala Met Asn Lys Val Ala Ile 70 gct cag gcg gca gcg ctt gcg agt ttg Ala Gln Ala Ala Ala Leu Ala Ser Leu gaa cgg gtg gag gaa acc gtc gaa aag Glu Arg Val Glu Glu Thr Val Glu Lys 100 105 ctt ggt get gcg ccg acg cag gcc aat Leu Gly Ala Ala Pro Thr Gin Ala Asn 115 120 ggc gcc gct gag ttg gcg gct aaa ttg Gly Ala Ala Glu Leu Ala Ala Lys Leu 130 135 cgc gcg ttc ccc gag ggt gcg cgc att Arg Ala Phe Pro Glu Gly Ala Arg Ile 145 150 act gac aag ctg ctg cgc gcg tgg gag Thr Asp Lys Leu Leu Arg Ala Trp Glu 165 tagtctttgg cgttttgcgg tgc <210> 398 <211> 174 <212> PRT <213> Corynebacterium glutamicum <400> 398 Met Glu Lys Val Pro Asn Asp Val Val 1 Phe Glu Phe Asn Arg Ala Asp Asp Thr His Arg His Asp Asn Val Ile Gly Leu 40 Gly Leu Ala Gly Leu Arg Val Gly Tyr 55 Ile Ala Ala Met Asn Lys Val Ala Ile 70 Ala Gin Ala Ala Ala Leu Ala Ser Leu Glu Arg Val Glu Glu Thr Val Glu Lys 100 105 Leu Gly Ala Ala Pro Thr Gin Ala Asn 115 120 Gly Ala Ala Glu Leu Ala Ala Lys Leu Phe 75 tct Ser gat Asp gtc Val gag Glu gtg Val 155 ate Ile Ala gcc Ala gct Ala tgg Trp cac His 140 acc Thr aat Asn PCT/IBOO/00923 Ser Ala ttg atg 288 Leu Met tca gcg 336 Ser Ala ggc gag 384 Gly Glu gtg att 432 Val Ile gag gaa 480 Glu Glu 160 522 Gly Val Thr Phe Phe Ser Asp Val Glu Leu Ala Phe Gly Ala Ala Ala Trp His Asp Thr Ser Asn Val Asp Val Leu 125 Gly WO 01/00843 WO 0100843PCTJ/IBOO/00923 Arg Ala Phe Pro Giu Gly Ala Arg Ile Ser Val 145 150 155 Thr Asp Lys Leu Leu Arg Ala Trp Glu Ala Ile 165 170 Thr Asn Ala Giu Asn Ala Gly <210> 399 <211> 1221 <212> DNA <213> Corynebacterium glutamdcumf <220> <221> <222> <223>

CDS

(101)..(1198) RXA01105 <400> 399 ttgtcatcaa ctttgccaac gccgaagatc ttccagcgca cggcgaagca atccgtgcac gctttgaaaa cctccccacc accgacgagg cctaagaaaa atg acc aaa att Met Thr Lys Ile 1 ttg agc gat ttg Leu Ser Asp Leu ttg cgt gaa gaa ctg cgc ggt gag Leu Arg Glu Glu Leu Arg Gly Giu cac gct tac His Ala Tyr ggc gca ccc Gly Ala Pro cct tac cca Pro Tyr Pro ctc aac gtt gat Leu Asn Val Asp cgc ctc aac acc aac gaa 'aac Arg Leu Asn Thr Asn Giu Asn ccg tca gag gca ttg gtc gct gac ttg Pro Ser Glu Ala Leu Val Ala Asp Leu gcc acc gtg Ala Thr Val gat aag Asp Lys atc gcc acc gag Ile Ala Thr Glu aac cgc tac cca Asn Axg Tyr Pro cgc gat gct gtg Arg Asp-Ala Val ctg cgt gat gag Leu Arg Asp Giu gct gcg tac atc Ala Ala Tyr Ilie aag caa acc: ggc Lys Gin Thr Gly gct gtc acc agg Ala Val Thr Arg ga t Asp aac ctg tgg gct Asn Leu Trp Ala aat ggt tcc aat Asn Gly Ser Asn gaa att Giu Ile 100 ctg cag cag Leu Gin Gin gga ttc caa Gly Phe Gin 120 ctg cag gct ttt Leu Gin Ala Phe gga cct gga cgc Gly Pro Gly Arg acc gcg ttg Thr Ala Leu 115 aaa ggc acc Lys Gly Thr ccc agc tat tcc atg cac cca att ttg Pro Ser Tyr Ser Met His Pro Ile Leu 125 gc t Ala 130 cac act His Thr 135 gaa ttc att gcg Giu Phe Ilie Ala tcc cga ggt gct gat ttc cgc atc gat Ser Arg Gly Ala Asp Phe Arg Ile Asp 145 WO 01/00843 WO 0100843PCT/IBOO/00923 atg gat gtg gcg ctg gaa gaa att cgt gca aag cag cct gac att gtt 595 Met Asp Val Ala Leu Glu Giu Ile Arg Ala Lys Gin Pro Asp Ile Val 150 155 160 165 ttt gtc-acc acc ccg aac aac ccg acc ggt gat gtg acc tcg ctg gac 643 Phe Val Thr Thr Pro Asn Asn Pro Thr Gly Asp Val Thr Ser Leu Asp 170 175 180 gat gtt gag cgc atc atc aac gtt gcc cca ggc atc gtg atc gtg gat 691 Asp Val Giu Arg Ile Ile Asn Val Ala Pro Gly Ile Val Ile Val Asp 185 190 195 gaa gct tat gcg gaa ttc tcc cca tca cct tca gca acc act ctt ctg 739 Giu Ala Tyr Ala Giu Phe Ser Pro Ser Pro Ser Ala Thr Thr Leu Leu 200 205 210 gag aag tac cca acc aag ctg gtg gtg tcc cgc acc atg agt aag gct 787 Giu Lys Tyr Pro Thr Lys Leu Val Val Ser Arg Thr Met Ser Lys Ala 215 220 225 ttt gat ttc gca ggt gga cgc ctc ggc tac ttc gtg gcc aac cca gcg 835 Phe Asp Phe Ala Gly Gly Arg Leu Gly Tyr Phe Val Ala Asn Pro Ala 230 235 240 245 ttt atc gac gcc gtg atg cta gtc cgc ctt ccg tat cat ctt tca gcg 883 Phe Ile Asp Ala Val Met Leu Val Arg Leu Pro Tyr His Leu Ser Ala 250 255 260 ctg agc caa gca gcc gca atc gta gcg ctg cgt cac tcc gct gac acg 931 Leu Ser Gin Ala Ala Ala Ilie Val Ala Leu Arg His Ser Ala Asp Thr 265 270 275 ctg gga acc gtc gaa aag ctc tct gta gag cgt gtt cgc gtg gca gca 979 Leu Gly Thr Val Glu Lys Leu Ser Val Glu Arg Val Arg Val Ala Ala 280 285 290 cgc ttg gag gaa ctg ggc tac gct gtg gtt cca agt gag tcc aac ttt 1027 Arg Leu Glu Glu Leu Gly Tyr Ala Val Val Pro Ser Glu Ser Asn Phe 295 300 305 gtg ttc ttt gga gat ttc tcc gat cag cac gcg gca tgg cag gca ttt 1075 Val Phe Phe Gly Asp Phe Ser Asp Gin His Ala Ala Trp Gin Ala Phe 310 315 320 325 ttg gat agg gga gtg ctc atc cgc gat gtg gga atc gct ggg cac ttg 1123 Leu Asp Arg Gly Val Leu Ile Arg Asp Val Gly Ile Ala Gly His Leu 330 335 340 cgc act acc att ggt gtg cct gag gaa aat gat gcg ttt ttg gac gca 1171 Arg Thr Thr Ile Gly Val Pro Glu Glu Asn Asp Ala Phe Leu Asp Ala 345 350 355 gct gca gag atc atc aag ctg aac ctg taagagagaa gaatttttca 1218 Ala Ala Glu Ile Ile Lys Leu Asn Leu 360 365 WO 01/00843 PCT/IB00/00923 tga 1221 <210> 400 <211> 366 <212> PRT <213> Corynebacterium glutamicum <400> 400 Met Thr Lys Ile Thr Leu Ser Asp Leu Pro Leu Arg Glu Glu Leu Arg 1 5 10 Gly Glu His Ala Tyr Gly Ala Pro Gin Leu Asn Val Asp Ile Arg Leu 25 Asn Thr Asn Glu Asn Pro Tyr Pro Pro Ser Glu Ala Leu Val Ala Asp 40 Leu Val Ala Thr Val Asp Lys Ile Ala Thr Glu Leu Asn Arg Tyr Pro 55 Glu Arg Asp Ala Val Glu Leu Arg Asp Glu Leu Ala Ala Tyr Ile Thr 70 75 Lys Gin Thr Gly Val Ala Val Thr Arg Asp Asn Leu Trp Ala Ala Asn 90 Gly Ser Asn Glu Ile Leu Gin Gin Leu Leu Gin Ala Phe Gly Gly Pro 100 105 110 Gly Arg Thr Ala Leu Gly Phe Gin Pro Ser Tyr Ser Met His Pro Ile 115 120 125 Leu Ala Lys Gly Thr His Thr Glu Phe Ile Ala Val Ser Arg Gly Ala 130 135 140 Asp Phe Arg Ile Asp Met Asp Val Ala Leu Glu Glu Ile Arg Ala Lys 145 150 155 160 Gin Pro Asp Ile Val Phe Val Thr Thr Pro Asn Asn Pro Thr Gly Asp 165 170 175 Val Thr Ser Leu Asp Asp Val Glu Arg Ile Ile Asn Val Ala Pro Gly 180 185 190 Ile Val Ile Val Asp Glu Ala Tyr Ala Glu Phe Ser Pro Ser Pro Ser 195 200 205 Ala Thr Thr Leu Leu Glu Lys Tyr Pro Thr Lys Leu Val Val Ser Arg 210 215 220 Thr Met Ser Lys Ala Phe Asp Phe Ala Gly Gly Arg Leu Gly Tyr Phe 225 230 235 240 Val Ala Asn Pro Ala Phe Ile Asp Ala Val Met Leu Val Arg Leu Pro 245 250 255 Tyr His Leu Ser Ala Leu Ser Gin Ala Ala Ala Ile Val Ala Leu Arg 260 265 270 WO 01/00843 WO 0100843PCT/iBOOIOO923 His Val Ser 305 Ala Ile Ala Gly Leu 295 Phe Asp Thr Ala Thr 280 Giu Phe Arg Thr Giu 360 Ser 285 Ala Asp Arg Giu Asn 365 Vai Giu Val Val Gin His Asp Vai 335 Giu Asn 350 Leu <210> <211> 1449 <212> DNA <213> Corynebacteriun giutamicun <220> <221> CDS <222> (101)..(1426) <223> RXA01106 <400> 401 ggtaaacatg cgggcttaag aacttgtgtt gaggccgctt aagaatttcg attcaacctt ttaagggaga acttttcgcc gac Asp cca Pro gtt Vali Gly gag Giu tcg Ser tcc Ser tgg Trp cgc Arg cgc Arg aac Asn cgc Arg aag Lys tc t Ser ggc Gly ccc Pro acc Thr gtc Val1 ggattcgggc accgagctcg atg ttg aat gtc act Met Leu Asn Val Thr 1 atc cga cgt gct ttg Ile Arg Arg Ala Leu ccc ata gtg cag cct Pro Ile Vai Gin Pro gct gct ttg gat tac Ala Ala Leu Asp Tyr gtg cgg gtg cca gct Val Arg Val Pro Ala ccg ttg gtg cgt gaa Pro Leu Vai Arg Giu gtt cac gct gag caa Val His Ala Giu Gin 100 115 163 211 259 307 355 403 WO 01/00843 aag cca tc Lys Pro S( gag cgt tt Glu Arg P1 1: aat gcg gt Asn Ala Vi 135 gag gct g5 Glu Ala G] 150 cac ggt g5 His Giy G' ggt gtt gi Gly Val Ar ctg gct ti Leu Ala 1) 2( act gga c( Thr Giy Pi 215 gga gtg gt Gly Vai Vi 230 ctt gct gi Leu Ala Ai agc caa g( Ser Gin A: tcc gag ci Ser Giu G] 2E tca atc ac 1027 Ser Ile TI 295 cag agt g5 1075 Gin Ser G] 310 acc Thr att Ile tca Ser tcC Ser 155 cac His tgg Trp gac Asp atc Ile gat Asp 235 gcc Ala gat Asp aag Lys gcc Ala ctt Leu 315 act Thr gat Asp agc Ser 140 ctt Leu ccc Pro gct Ala gct Ala ttt Phe 220 tct Ser aac Asn gtc Val gac Asp gag Glu 300 gtc Val ctt Leu 110 gtg Va1 att Ile gtt Val att Ile ggc Gly 190 ggt Gly acc Thr gct Ala gtc Val gct Ala 270 aac Asn gtc Val gac Asp ctg tca Ser gga Gly atg Met gcg Ala ttg Leu 175 ggc Gly ctc Leu gct Ala ggc Gly aac Asn 255 gcg Ala agg Arg gca Ala att Ile gaa Glu ggt Gly tac Tyr act Thr 145 cct Pro gcg Ala cag Gin cct Pro aag Lys 225 aca Thr gcc Ala gtg Val atc Ile gct Ala 305 gtg Val cac His ggc acc Gly Thr 115 gtt cca Val Pro 130 gtc cca Val Pro cct cag Pro Gin tgt tcc Cys Ser gcc gtg Ala Val 195 gtg gat Val Asp 210 cgc ctg Arg Leu gaa atc Glu Ile tac gat Tyr Asp ctc atc Leu Ile 275 gag gcg Glu Ala 290 ttg cgc Leu Arg ggt atc Gly Ile act gag Thr Glu PCTiBOO/00923 gtc act 451 Val Thr ggc ggc 499 Gly Gly gct caa 547 Ala Gin gct gag 595 Ala Glu 165 atc ttg 643 Ile Leu 180 gcg ttg 69i Ala Leu atg atc 739 Met Ile gtc agg 787 Val Arg gct gtg 835 Ala Val 245 ctg atc 883 Leu Ile 260 act gac 931 Thr Asp cgt tac 979 Arg Tyr ggg gcc Gly Ala caa gta Gin Val 325 aac gcg Asn Ala gcc gat caa tac gca gcg gaa cac 1123 Ala Asp Gln Tyr Ala Ala Giu His Leu WO 01/00843 cgc gcc 1171 Arg Ala gat ttc 1219 Asp Phe gtg ctg 1267 Val Leu 375 cac acg 1315 His Thr 390 ctg aag 1363 Leu Lys ctt cca 1411 Leu Pro acc acc 1449 Thr Thr 330 gca gag Ala Glu 345 cca gta Pro Val acc tct Thr Ser ctt cgc Leu Arg gtc tcg Val Ser 410 cac ggc His Gly 425 gag gcc Glu Ala cag acc acc Gin Ile Thr cca ctg ggt Pro Leu Gly 365 gga tcc gct Gly Ser Ala 380 cca gtc aac Pro Val Asn 395 cag gtt gtc Gin Val Val gaa gca atc Glu Ala Ile aac Asn 350 ga t Asp cgt Arg ctc Leu atc Ile cg t Arg 430 335 gcg ggt Ala Gly tac tcc Tyr Ser ttc tcc Phe Ser att gaa Ile Glu 400 aac ttt Asn Phe 415 gca cgc ttc Phe 355 tcc Ser c ta Leu gag Glu gcc Ala aac PCTIBOO/00923 340 gtg ggc Val Gly aac cac Asn His tcc acg Ser Thr gct gct Ala Ala 405 gaa gat Glu Asp 420 ctc ccc Ala Arg Phe.Glu Asn Leu Pro taagaaaaat gaccaaaatt act <210> 402 <211> 442 <212> PRT <213> Corynebacterium glutamicun <400> 402 Met Leu Asn Val Thr Asp Leu Arg 1 Ile Arg Arg Ala Leu Pro Arg Gly C Pro Ilie Val Gin Pro Val Val Glu Ala Ala Leu Asp Tyr Gly Glu Lys 1 Val Arg Val Pro Ala Glu Val Ile Pro Leu Val Arg Glu Ser Ile GluC Lys Ser Gly Pro Thr Val WO 01/00843 WO 0100843PCTAiBOO/00923 Val Gly Tyr Thr 145 Pro Ala Gin Pro Lys 225 Thr Ala Val Ile Ala 305 Val His Ala Ala Ala 385 Tyr Gin Thr Gly Gin Giu 165 Leu Leu Ile Arg Val 245 Ile Asp Tyr Ala Val1 325 Ala Gly His Thr Al a 405 Lys Giu Asn Giu 150 His Gly Leu Thr Giy 230 Leu Ser Ser Ser Gin 310 Ala Arg Asp Val1 His 390 Leu Pro Arg Ala 135 Ala Gly Val Ala Gly 215 Val1 Ala Gin Giu Ile 295 Ser Asp Ala Phe Leu 375 Thr Lys Ser Glu 105 Phe Met 120 Vai Tyr Gly Val Gly Trp, Asp Giu 185 Tyr Gly 200 Pro Gly Val Gly Asp Ala Ala Glu 265 Gin Leu 280 Thr Arg Gly Ile Gin Tyr Val Ala 345 Ser Pro 360 Pro Thr Phe Leu Asp Val His Pro Pro Asn Pro 170 Val Asp Asn Thr Ser 250 His Ala Asn Val1 Ala 330 Glu Val1 Ser Arg Ser 410 Thr Ile Ser Ser 155 His Trp Asp Ile Asp 235 Ala Asp Lys Ala Leu 315 Ala Gin Pro Gly Pro 395 Gin Thr Asp Ser 140 Leu Pro Ala Al a Phe 220 Ser Asn Va 1 Asp Giu 300 Val1 Glu Ile Leu S er 380 Val Val1 Glu Arg 125 Val Val Thr Val Ala 205 Val Glu Ala Met Val1 285 Arg Asp His Thr Gly 365 Ala Asn Val1 Leu 110 Val Ile Val Ile Gly 190 Gly Thr Ala Val1 Ala 270 Asn Val Asp Leu Asn 350 Asp Arg Leu Ile Ser Gly Met Ala Leu 175 Gly Leu Ala Gly Asn 255 Ala Arg Ala Ile Giu 335 Ala Tyr Phe Ile Asn 415 Pro Leu Asn Ser 160 Ala Gly Giu Ala Pro 240 Val1 Ser Glu Giu Ser 320 Ile Gly Ser Ser Glu 400 Phe WO 01/00843 PCTIIBOO/00923 Ala Asn Ala Giu Asp Leu Pro Ala His Gly Glu Ala Ile Arg Ala Arg 420 425 430 Phe Glu Asn Leu Pro Thr Thr Asp Giu Ala 435 440 <210> 403 <211> 876 <212> DNA <213> Corynebacterium glutaiicun <2 <221> CDS <222> (101)..(853) <223> RXC00930 <400> 403 acggcgcacc acacatttgg tggtgtattg agctatctct gggctgcgtg agaaaccatt ttccggtgga tgatggaagc tagacgacga aagggagcat atg tct ggc cac tca Met Ser Gly His Ser 1 gct aac Ala Asn gcg Ala gaa Glu gg t Gly gc c Al a cg t Arg tac Tyr acc Thr aaa Lys 120 acc Thr aag Lys aag Lys ccg Pro gct Ala ggC Gly tac Tyr cgt Arg ggc Gly ggt Gly gc t Ala aac Asn cca Pro aac Asn gg t Gly ggc Gly gct Ala gag Giu gta Val1 atc Ile acg Thr ga t Asp ggc Gly gtt Val1 acc Thr tcc Ser caa Gin gac Asp gtt Val gat Asp atc Ile gac Asp atg Met gtt Val1 tcc Ser 130 ggC Gly gaa gat gac gtg ctc atg gct gtt ctt gaa gct ggt gct gaa gaa gtc WO 01/00843 Glu Asp Ac 150 aac gac ai Asn Asp Ai cag gct gt Gin Ala Vi tc t Ser gg t Gly ga t Asp 230 aca Thr Val Leu ggc gat Gly Asp 170 cgc gac Arg Asp 185 gac ttc Asp Phe aag atc Lys Ile aac gtc Asn Val gaa aac Glu Asn 250 gag Glu g tg Val1 Val Leu Glu A:

PJ

la Glu a act ro Thr aa gta Lu Val 195 g gac eu Asp L0 aa gat Lu Asp at gag sp Glu PCTIIB0O/00923 Glu Val 165 gac att 643 Asp Ile 180 gaa gat 691 Glu Asp gct gac 739 Ala Asp tcc gac 787 Ser Asp gtt ttg 835 Val Leu 245 gca tct gct cag gtc ccc ci Ala Ser Val Gin Val Pro L 2052 aag ctt gtg gac gcg ttg g Lys Leu Val Asp Ala Leu G: 220 225 acc aac atc gac ttg agc g~ Thr Asn Ile Asp Leu Ser A~ 240 tagttcgtat tttccgcact ccg <210> 404 <211> 251 <212> PRT <213> Corynebacteriun glutamicun <400> 404 Met Ser Gly His Ser Lys Trp Ala Thr 1 Asn Asp Ala Lys Arg Gly Lys Glu Phe Glu Val Ala Ala Arg Thr Gly Gly Gly Leu Asp Asp Met Ile Lys Lys Ala Lys 55 Asn Ile Glu Arg Ala Arg Lys Arg Gly 70 Ala Asp Trp Met Asn Ile Met Tyr Glu Ala Met Leu Ile Glu Cys Leu Thr Asp 100 105 Glu Val Arg Thr Ala Met Thr Lys Asn 115 120 Gly Ser Val Ser Tyr Met Phe Thr Arg 130 135 His Leu Ser Ser Glu Giy Asn Asn Val 140 Ala Ile Thr Asp Gly Val Thr Ser Gin WO 01/00843 Lys Gly Ac 145 Gly Ala GJ Ala Pro TI Ilie Glu Vi 1! Pro Leu A, 210 Leu Glu A., 225 Ser Asp G: Leu Glu Asp 180 Glu Ala Ser Val1 Asp ASn Val Ser 200 Arg Gin Leu Val Gly Arg 185 Asp Lys Asn Glu Leu Asp 170 Asp Phe Ile Val1 Asn 250 Met 155 Leu Ala Arg Phe Tyr 235 Asp PCT/iBOO/00923 Glu Ala 160 Thr Cys 175 Ala Gly Gin Val Asp Ala Asp Leu 240 <210> 405 <211> 547 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(547) <223> RXC01096 <400> 405 accgtgaaac aaaccggcgg tgcgtgccac actggtgcc gttttgctgt aaaagcaaca acgattaagg aagaaatctt c tg Leu tca Ser agt Ser gcg Ala aag Lys gtg Val ggC Gly egg Trp tct Ser gc t Ala cgg Arg ctg Leu att Ile g ta Val1 at Ile t tg Leu cgc Arg 75 tcC Ser gc t Ala atc Ile gg C Gly gct Ala att Ile CtC Leu acacatgttt cgacaatgac atg aag cca cgc gtg Met Lys Pro Arg Val 1 gtt gtc egg atc agc Val Val Trp Ile Ser gac gat aaa tca ggt Asp Asp Lys Ser Gly tcC aca gaa atc atg Ser Thr Glu Ile Met gcc gcg ttg gtg ctc Ala Ala Leu Val Leu tcg gcg ctg atc gcg Ser Ala Leu Ile Ala acc caa gac cca gac Thr Gin Asp Pro Asp WO 01/00843 gca gaa cgg gcc cga 8CC ctg ctg acc Ala Giu Arg Ala Arg Thr Leu Leu Thr 105 110 gct aat tcc gga acc ctg ctg tct gat Ala Asn Ser Gly Thr Leu Leu Ser Asp 120 125 acc acc cat cca ctg gcg gca gtg gta Thr Thr His Pro Leu Ala Ala Val Val 135 140 <210> 406 <211> 149 <212> PRT <213> Corynebacterium giutanicun <400> 406 Met Lys Pro Arg Val Leu Ser Ala Leu 1 5 Val Val Trp Ilie Ser Ser Arg Met Asn 25 Asp Asp Lys Ser Gly Ser Vai Thr Gin Ser Thr Giu Ilie Met Ala Leu Ala Leu Ala Ala Leu Val Leu Lys Arg Met Gly 70 Ser Ala Leu Ile Ala Val Gly Ala Ser Thr Gin Asp Pro Asp Ala Glu Arg Ala 100 105 Val Ala Ser Gin Lys Ala Asn Ser Gly 115 120 Gu Ile Ile Asn Thr Thr Thr His Pro 130 135 Ile Gly Cys Ala Leu 145 <210> 407 <211> 1020 <212> DNA <213> Corynebacterium glutanicun <220> <221> CDS <222> (101)..(997) <223> RXCO1656 PCTIIBOO/00923 95 100 tcc ggt gtg gcc tca cag aag 451 Ser Gly Val Ala Ser Gin Lys 115 tgg gcg gag atc atc aat 8cc 499 Trp Ala Gu Ile Ile Asn Thr 130 gcc atg att ggc tgc gcg cta 547 Ala Met Ile Gly Cys Ala Leu 145 Gly Glu Ala Ala Gly Ala Thr 110 Asp Val1 Ala Ala Thr Phe Gly Leu S er Trp, Ala Leu Phe Trp Ala Ile Leu Gly Ala Met WO 01100843 WO 0100843PCTA0B00/00923 <400> 407 tcggcaagca tggcttcatc gtcctcaaaa aagtggtcta atgcaagtga ctgaaagtgg atctaccgct agtccacttt gtggcgttgg atcatctgtc atg acc gaa act caa Met Thr Giu Thr Gin gaa act tac caa Giu Thr Tyr Gin gca Ala acc act cgt gtg Thr Thr Arg Val cgc ggc ctt gcc Arg Gly Leu Ala gac atg Asp Met ctc aag ggt Leu Lys Gly gtg atc atg gat gtg gtc acc cct gaa Val Ile Met Asp Val Val Thr Pro Giu caa gcg cgc Gin Ala Arg gag cgc gtt Giu Arg Val atc gcc gaa gat gca ggt gcc agc gca gtt atg gca Ilie Ala Giu Asp Ala Gly Ala Ser Ala Val met Ala C tC Leu ccc gcc Pro Ala gat atc cgt tct Asp Ile Arg Ser ggc ggc gtt gct Gly Gly Val Ala atg agt gat cct Met Ser Asp Pro gac Asp ctg atc gaa gga Leu Ilie Giu Gly gtc aat gcg gtc Val Asn Ala Val atc ccg gtc atg Ile Pro Val Met aaa gct cgc atc Lys Ala Arg Ile gg t Gly cac ttc gtg gaa His Phe Val Giu cag gtt ctg gaa Gin Val Leu Giu gct ctc Ala Leu 100 ggt gtt gat Gly Val Asp acg cac cac Thr His His 120 t tc Phe 105 atc gac gag tcc Ile Asp Glu Ser gtt ctc agc cct Val Leu Ser Pro gcc gac tac Ala Asp Tyr 115 gtC tgt ggc Val Cys Gly atc aac aag tgg Ile Asn Lys Trp ttc gac gtt cct Phe Asp Val Pro t tc Phe 130 gcg acc Ala Thr 135 aac ctc ggc gaa Asn Leu Gly Giu ttg cga cgc atc Leu Arg Arg Ile acc Thr 145 gaa ggc gct gca Giu Gly Ala Ala atg atc cgt tcc aag Met Ile Arg Ser Lys 150 gtc cgt cac ctg cgc Val Arg His Leu Arg 170 ggC G ly 155 gaa gcc ggc acc Giu Ala Gly Thr gat gtc tct gaa Asp Val Ser Giu acc atc cgc ggc Thr Ile Arg Gly atc aat cgc ctg Ile Asn Arg Leu cgc tcc Arg Ser 180 ctg gat gag gat gaa ctc ttc gtc Leu Asp Giu Asp Giu Leu Phe Val 185 gcc aag gaa ttc Ala Lys Giu Phe cag gca cca Gin Ala Pro 195 cct gtg gtC Pro Val Val tac gac ctg Tyr Asp Leu 200 gtc cgc gaa gtc Val Arg Giu Val tcc acc ggc aag Ser Thr Gly Lys c tc Leu 210 acc ttc gtt gca ggt ggc gtc gca acc cca gcc gac gct gca ctc gtg WO 01/00843 Thr Phe Val Ala Gly Gly Val Ala 215 220 cgc caa atg ggc gcc gaa ggc gtc Arg Gin Met Giy Ala Glu Gly Val 230 235 tcc ggc aat cca gcc gcc cgc gcc Ser Gly Asn Pro Ala Ala Arg Ala 250 ctt ttc gac gac ccc tcc gtc att Leu Phe Asp Asp Pro Ser Val Ile 265 gaa gcc atg gtg ggc atc aac gta Giu Ala Met Val Gly Ilie Asn Val 280 285 ctc gcc gag cgc ggc tgg tgatcgtt( 1020 Leu Ala Giu Arg Gly Trp 295 <210> 408 <211> 299 <212> PRT <213> Corynebacterium giutamicum rhr Pro Ala Asp Ala 225 ttt gtc ggc tcc ggc Phe Val Giy Ser Gly 240 Ica gcg atc gtc aag kia Ala Ile Val Lys 255 gcc gac gta tcc cgc kla Asp Val Ser Arg 270 tcc gac gtt cca gca Ser Asp Vai Pro Ala 290 gg agttttagct ctc PCTI[BOO/00923 Ala Leu Val <400> 408 Met Thr Glu Thr Gin Glu Thr Tyr Gin Ala Gin Giu Ser Val G lu Al a 115 Val Giy Met Arg Val1 Pro Ala Leu Tyr Gly Ala Leu Ile Pro Asp Lys Gly Thr Ala Met 150 Lys Ala Ala Leu Val1 His Thr 135 Ile Ala 10 Val Ala Arg Gly Gly 90 Ilie Asn Gly Lys Arg Asp Ser G ly Asn Val Ser Lys 125 Leu Ala Val1 Val1 Ala Gly Ala Glu Giu 110 Phe Arg Gly Lys Val Val Val Val Ala Val Asp Arg Thr WO 01/00843 Asp Val Ser Glu Ala Val Arg His Leu Arg Thr 165 170 Asn Arg Leu Arg Ser Leu Asp Glu Asp Giu Leu 180 185 Glu Phe Gin Ala Pro Tyr Asp Leu Val Arg Glu 195 200 Lys Leu Pro Val Val Thr Phe Val Ala Gly Gly 210 215 Asp Ala Ala Leu Val Arg Gin met Gly Ala Giu 225 230 235 Ser Gly Ile Phe Lys Ser Gly Asn Pro Ala Ala 245 250 Val Lys Ala Ala Thr Leu Phe Asp Asp Pro Ser 260 265 Ser Arg Gly Leu Gly Giu Ala Met Val Gly Ilie 275 280 Pro Ala Pro His Arg Leu Ala Giu Arg Gly Trp 290 295 <210> 409 <211> 1065 <212> DNA <213> Corynebacterium glutamicum <22 0> <221> CDS <222> (1042) <223> RXC01158 <400> 409 taggacgttc aaggaattgg ctgaatcaac aagcgccaag agttatctca aaaaagaaga agaagtctcc tacgggagag Gly Ala 190 Ser Thr Phe Ala Ala 270 Ser PCTIBOO/00923 Asp Ile 175 Ala Lys Thr Gly Pro Ala Val Gly 240 Ala Ile 255 Asp Val Asp Val c ac His C tc Leu cag Gin cc t Pro gag Glu acc Thr act Thr tcc Ser cga Arg atc Ilie Gly tgg Trp gtggttaagc gccctcggcg atg tcc att gtt gag Met Ser Ile Val Glu 1 gct ctg gcg ggc atc Ala Leu Ala Gly Ile gat ttc tca ttt tgg Asp Phe'Ser Phe Trp ccg tac tgt tct ctg.

Pro Tyr Cys Ser Leu gag gaa tgt cga ctg Glu Glu Cys Arg Leu WO 01/00843 WO 0100843PCTIiBOO/00923 ctc Leu gca acc ggc ccg Ala Thr Giy Pro ttt Phe gat cca ttc atg Asp Pro Phe Met cgc ctt aaa gta Arg Leu Lys Val gcg ttg gtg ggt Ala Leu Val Giy gtt ctt ggc tca Val Leu Gly Ser gtg tgg ctg agc Val Trp Leu Ser cag ctg Gin Leu 100 tgg ggc ttt Trp Gly Phe gca atc ttc Ala Ilie Phe 120 acc cca ggt ttg Thr Pro Gly Leu aag aat gag cgc Lys Asn Glu Arg cgt tac acc Arg Tyr Thr 115 ggt gct gtt Giy Aia Vai gtc acg att gct Val Thr Ile Ala gtg ctg ttt gtc Val Leu Phe Val ctt gcg Leu Ala 13 tac ttc gtc gtt Tyr Phe Val Val tat ggt ttg gag Tyr Gly Leu Glu ctc ctt acc att Leu Leu Thr Ile gga gac acc cag Giy Asp Thr Gin gca Al a 155 gcg gcc ctg act Ala Ala Leu Thr gat aag tac ttc Asp Lys Tyr Phe ttc ttg ctc gcg Phe Leu Leu Ala t tg Leu 170 ttg gcg att ttc Leu Ala Ile Phe gtg agc ttc gaa Val Ser Phe Glu gtt cca Val Pro 180 ctg gtg atc Leu Val Ilie att aaa gat Ile Lys Asp 200 atg ctc aac att Met Leu Asn Ile ggt atc ttg cct Giy Ile Leu Pro tac gat gcc Tyr Asp Ala 195 gtg ttc gct Val Phe Ala aag cga cgc atg Lys Arg Arg Met atc atg att ttg Ile Met Ile Leu ttc Phe 210 gct ttc Ala Phe 215 atg aca ccc ggc Met Thr Pro Gly cag Gin 220 gat cct ttc acc Asp Pro Phe Thr ttg gtg ttg gcg Leu Val Leu Ala ctt tca ctc acc gtt Leu Ser Leu Thr Val 230 aac gac aaa cgc cgg Asn Asp Lys Arg Arg 250 c tg Leu 235 gta gag ctt gcc Val Giu Leu Ala cag ttc tgt cgc Gin Phe Cys Arg gac aag aag cgc Asp Lys Lys Arg gaa tgg ctt gat Giu Trp Leu Asp ggc gat Gly Asp 260 gac ctc tct Asp Leu Ser cca agc cca Pro Ser Pro 280 gca Ala 265 tca cca ctg gat Ser Pro Leu Asp tct gct ggt gga Ser Ala Gly Gly gaa gat gct Giu Asp Ala 275 cgg atg ctg Arg Met Leu gtc gaa acc cca Val Giu Thr Pro gcg gtg gag cct Ala Val Giu Pro aac cca agt ggg gag gcg tcg ata agc tat aaa ccc ggg cgc gcc gac 1027 Asn Pro 295 Ser Gly Glu Ala Ser 300 Ile Ser Tyr Lys Gly Arg Ala Asp WO 01/00843 ttc ggt gac gtg ctc tagggcctag ccaggtaccc tta 1065 Phe Gly Asp Val Leu 310 <210> 410 <211> 314 <212> PRT <213> Corynebacterium giutainicum PCT/IBOO/00923 <400> 410 Met Ser Ilie Val Giu His Ile Lys Giu 1 Ala Asp Pro Glu Arg Trp, Giu Val Phe 145 Asp Ser Leu Leu Met 225 Gin Leu Phe Tyr Glu Leu Leu Arg Gly 130 Leu Lys Phe Pro Phe 210 Leu Phe Ala Ser Cys Cys Lys Ser Arg 115 Gly Leu Tyr Giu Tyr 195 Vai Val Cys Gly Phe Ser Arg Val Gin 100 Tyr Ala Thr Phe Val 180 Asp Phe Leu Arg Val Ile Ala 55 Al a Leu Gly Ile Al a 135 Gly Leu Val1 Lys Phe 215 Ser Asp Gly Pro 40 Giu Thr Val Phe Phe 120 Tyr Asp Leu Ile Asp 200 Met Leu Lys Thr 25 Thr Ser Gly Gly Ile 105 Val1 Phe Thr Ala Gly 185 Lys Thr- Thr Arg Phe Ile Leu Arg Pro Met Thr Thr Val Gin Leu 170 Met Arg Pro Val1 Arg 250 Arg Ile G ly Trp Phe 75 Val1 Pro Ile Val Ala 155 Leu Leu Arg Gly Leu 235 Asp Arg Gly Giu Ala Asp Leu Gly Ala Ala 140 Ala Ala Asn Met Gin 220 Val Lys Arg Phe Leu Met Pro Gly Leu Val1 125 Tyr Ala Ile Ile Ile 205 Asp Giu Lys Leu Ilie Leu Ser Phe Ser Met 110 Val1 Gly Leu Phe Val1 190 Ile Pro Leu Arg Ile Tyr Asp Ser Leu Val Asn Phe Glu Gly 160 Val1 Ile Ile Thr Leu 240 Glu 245 Trp Leu Asp Giy Asp Asp Leu Ser Ala Ser Pro Leu Asp Thr Ser Ala WO 01/00843 WO 0100843PCT/IBOO/00923 270 Glu Thr Pro Glu Ala Val Glu 285 Gly Gly Giu Asp Ala Pro Ser Pro Val 275 280 Pro Ser Arg Met Leu Asn Pro Ser Gly Giu Ala Ser 290 295 300 Pro Gly Arg Ala Asp Phe Gly Asp Val Leu 305 310 <210> 411 <211> 1413 <212> DNA <213> Coryriebacteriun giutaiicuxn Ile Ser Tyr Lys <220> <221> <222> <223>

CDS

(101)..(1390) RXA02458 <400> 411 ttgcggagcc acgactgatc ggtcaagtgc tatctcggtt gcatcaataa gcgaatcacc ggtggtgaac aaaacaaatc ttccacacat aacatctatt atg gtc ttt gtg tct Met Val Phe Val Ser 115 gat tcg tct atc Asp Ser Ser Ile ttg ccc att tgg Leu Pro Ile Trp gct ccg cgc gct Ala Pro Arg Ala cgc ggc Arg Gly ccc ata gtc Pro Ilie Val cgc gcc ctc Arg Ala Leu tcg Ser gac ctg gct atc Asp Leu Ala Ile ggt tcc aag tcg Gly Ser Lys Ser atc acc aac Ile Thr Asn acc atc att Thr Ile Ile atc ttg gct gcg ctc gca tca act cca Ile Leu Ala Ala Leu Ala Ser Thr Pro 259 307 gat gtc Asp Val ctt cgt agt cgt Leu Arg Ser Arg acc gat ctc atg Thr Asp Leu Met act Thr gat ggt cta cgc Asp Gly Leu Arg ctc gga atc acc att act gaa gag gca Leu Giy Ilie Thr Ile Thr Glu Giu Ala gtc gat cgc tac cgc gtt Val Asp Arg Tyr Arg Val 80 gag ccc gga cag Giu Pro Gly Gin tct gct ggc tcc Ser Ala Gly Ser gag tgt ggt ctt Glu Cys Gly Leu gct ggt Ala Gly 100 acg gtc atg Thr Val Met gtt cat ttt Val His Phe 120 ttt ttg cct cct Phe Leu Pro Pro gct gct ttc gct Ala Ala Phe Ala gat ggt cct Asp Giy Pro 115 atg acc agc Met Thr Ser 451 499 gat ggc gat cct Asp Gly Asp Pro gct cgt gtt cgt Ala Arg Val Arg WO 01/00843 att ttg gat Ile Leu Asp 135 ctg cct ttc Leu Pro Phe 150 gag att gat Giu Ile Asp tca gcg cct Ser Ala Pro cgt ctg Arg Leu tcc gca Ser Ala 215 cc:: ggt Pro Gly 230 aat gcg Asn Ala aag att Lys Ile cgt tcg Arg Ser ggt gaa 1027 Gly Giu 295 att gag 1075 Ile Glu 310 gcg ttg 1123 Ala Leu cat ctt 1171 His Leu gcg Ala act Thr gc t Ala cgt Arg 185 agc Ser att Ile a tc Ile ccg Pro cac His 265 ctt Leu tac Tyr gat Asp gcg Ala ggc Gly 345 cgt Arg aa t Asn 155 ggc Gly aaa Lys ccg Pro atc Ile ggt Gly 235 cta Leu cca Pro cgc Arg ctg Leu tcc Ser 315 gcg Ala gag Glu tcg Ser 140 gc t Ala tca Ser aat Asn cat His gaa Glu 220 cgg Arg gc t Al a atc Ile atg Met tcg Ser 300 gat Asp tcg Ser acg Thr ctt Leu gg t Gly tc t Ser 9gc Gly att Ile 205 gag Giu acc Thr gcc Ala aaa Lys ggc Gly 285 gtg Val atc Ile aca Thr gat Asp ggt Gly gag Giu cag Gin gtc Val 190 gag Giu tca Ser tgg Trp gc t Ala act Thr 270 tgc Cys act Thr ggt Gly gag Glu cgt Arg 350 gtg Val1 gtc Val ttt Phe 175 ac c Thr atg Met gaa Glu cgc Arg gcg Ala 255 act Thr gaa Glu ggt Gly gag Glu tct Ser 335 t tg Leu gag Glu cct Pro 160 gtt Val gt t Val1 acc Thr aat Asn at t Ile 240 gtc Val cag Gin gtt Val1 ccg Pro t tg Leu 320 cg t Arg gc t Ala gta Val 145 gag Glu tct Ser aag Lys gtc Val1 cag Gin 225 gag Glu act Thr cct Pro gag Glu gtt Val 305 acc Thr ttg Leu gcg Ala gac Asp gg t Gly ggt Gly cac His gat Asp 210 tgg Trp, ccg Pro ggt Gly ggc Gly ctg Leu 290 gc t Ala cc t Pro ac c Thr t tg Leu aac Asn ggc Gly ctt Leu gtc Val 195 a tg Met gtt Val1 gat Asp gga Gly gat Asp 275 gt t Val c tc Leu acc Thr ggt Gly act Thr 355 PCTIBOO/00923 aac aat 547 Asn Asn gtg gtt 595 Val Val 165 ttg ctt 643 Leu Leu 180 ggt ggt 691 Gly Gly ctt cgt 739 Leu Arg gtt cat 787 Val His ctt tct 835 Leu Ser 245 acc atc 883 Thr Ile 260gct at:: 931 Ala Ile gct cag 979 Ala Gin aag ggc Lys Gly gtg gcg Val Ala 325 att gct Ile Ala 340 gcg gag Ala Glu WO 01/00843 atc aac aaa ctt ggt gga aag tgc 1219 Ile Asn Lys Leu Gly Gly Lys Cys 360 365 att gag cct gcg tcg ctg cac ggt 1267 Ile Glu Pro Ala Ser Leu His Gly 375 380 cac cgt atg gct act gct ggt gcg 1315 His Arg Met Ala Thr Ala Gly Ala 390 395 gtt cag gtt gaa gac att aag acc 1363 Val Gin Val Giu Asp Ile Lys Thr 410 gaa aat gtt tgg gag gag atg gtt 1410 Glu Asn Val Trp Glu Glu Met Val 425 ccg 1413 <210> 412 <211> 430 <212> PRT <213> Corynebacteriun glutamicum <400> 412 Met Val Phe Val Ser Asp Ser Ser 1 5 Pro Arg Ala Arg Gly Pro Ilie Val Lys Ser Ile Thr Asn Arg Ala Leu Pro Ser Thr Ilie Ile Asp Val Leu3 Thr Asp Gly Leu Arg Ser Leu Gly 70 Asp Arg TIyr Arg Val Glu Pro Gly Cys Gly Leu Ala Gly Thr Val Met 100 Phe Ala Asp Gly Pro Val His Phe 115 120 Arg Pro Met Thr Ser Ile Leu Asp 1 130 135 PCT/IBOO/00923 gag ctt aag gat ggt ctg ttg Giu Leu Lys Asp Gly Leu Leu 370 gtg tgg cat tca tat gct gat Val Trp His Ser Tyr Ala Asp 385 att ggc ctc gcg gtt gat ggc Ile Gly Leu Ala Val Asp Gly 400 405 tcc aaa act ttc cct ggt ttt Ser Lys Thr Phe Pro Gly Phe 415 420 tagacgcagc tatgacgaat Ilie Ser 10 Ser Asp Ilie Leu krg Ser Ile Thr Uln Leu 90 krg Phe ksp Gly la Leu Asp Gly Ser Leu Ala Val1 Ala Arg Val Ala Ser Thr Met Val Glu Ala Val Glu WO 01/00843 WO 0100843PCT/iBOO/00923 Asn Asn Val Val 165 Leu Leu 180 Gly Gly Leu Arg Val His Leu Ser 245 Thr Ile 260 Ala Ilie Ala Gin Lys Gly Val Ala 325 Ile Ala 340 Ala Giu Leu Leu Aia Asp Asp Gly 405 Thr Aia Arg 185 Ser Ile Ile Pro His 265 Leu Tyr Asp Ala Giy 345 Leu Ala Ala Glu Trp 425 Ala S er Asn His Giu 220 Arg Ala Ile Met Ser 300 Asp Ser Thr Lys His 380 Gly Lys Gly Ser Gly Ile 205 Giu Thr Ala Lys Gly 285 Val Ile Thr Asp Cys 365 Gly Ala Thr Giy 420 Phe Giu Asn Val Glu Glu Met Val Gly 430 <210> 413 <211> 1266 <212> DNA <213> Corynebacterium glutainicum WO 01/00843 <220> <221> CDS <222> (101)..(1243) <223> RXA02790 <400> 413 tggatgctcg cacccgagcc cttgaaccac aatccacaga PCTfIBOO/00923 cacccaagat ttcgacgaga agggaaattt cccaggatga accaaatccg aaaccgccgg atg gag ccc gtc tac Met Giu Pro Val Tyr gta Val c tc Leu acg Thr gaa Glu gaa Giu aac Asn gaa Giu aac Asn gtc Val gca Ala 150 gat Asp cca Pro aag Lys att Ile cca Pro a tc Ile cga Arg aac Asn caa Gin cag Gin act Thr 135 gcc Ala ttg Leu ga t Asp cgc Arg atc Ile cat His cc t Pro gat.

Asp ccc Pro a tg Met gtt Vai 120 gtt Val g tg Val cag Gin tgg Trp cgc Arg ggt Gly gac Asp gaa Giu a tc Ile aac Asn aac Asn 105 gat Asp gtc Val acc Thr cag Gin gca Al a 185 caa Gin gcc Ala tat Tyr gg t Gly gtg Val1 gcg Ala gca Ala c tc Leu ggc Gly tgc Cys gtt Val 170 atc Ile att Ile tat Tyr tcc Ser 45 ata Ile aac Asn gta Val gca Ala att Ile 125 ac c Thr ggc Gly acc Thr gtg Val 1 atc Ile gcc Ala gtg Val1 Gly caa Gin ttc Phe ctg Leu gcc Ala atc Ile 145 tgc Cys gca Ala gga Gly gc t Ala acc Thr gtt Val1 cca Pro aca Thr t ac Tyr ctt Leu acc Thr 130 tac Tyr atc Ile gaa Giu act Thr ctc Leu aac Asn c tg Leu t tg Leu gcc Ala c tg Leu 100 cca Pro atg Met cag Gin gc t Ala ggt Gly 180 cca Pro cgc ctc gaa ggc ctg atc atg cct ggc caa tac gtg gtg gat cca tcc WO 01/00843 Arg Leu Glu 200 aac gac gcc Asn Asp Ala 215 cat ttc caa His Phe Gin 230 act cca tat Thr Pro Tyr cca gca gga Pro Ala Gly gcc gag cca Ala Giu Pro 280 tct gaa caa 1027 Ser Glu Gin 295 cca tgg aac 1075 Pro Trp Asn 310 gca gta tcc 1123 Ala Val Ser aac tgg ctg 1171 Asn Trp Leu aac gac acc 1219 Asn Asp Thr 360 agt ggc gtt 1266 Ser Gly Val 375 Ile atc Ile gac Asp 235 gtc Val gat Asp ctg Leu gca Ala gcc Ala 315 gca Ala gtc Val1 gag Giu agc Ser Met ctc Leu 220 atc Ile ac c Thr aag Lys caa Gin acc Thr 300 atg Met c tc Leu acc Thr cac His aac Asn 380 Giy gat Asp ggC G ly gca Ala gcc Ala 270 gac Asp gac Asp ggc Gly gcc Ala gac Asp 350 gcc PCTIIBOO/00923 Pro Ser gca aac 787 Ala Asn gga ctt 835 Gly Leu 245 gaa gca 883 Glu Ala 260 cgt ctc 931 Arg Leu ggt ctg 979 Gly. Leu gtc acc Val Thr atc gcc Ilie Ala 325 gaa gga Giu Gly 340 gtg ttc Vai Phe ttg aac Ala Asp Ile Giu Gin Ala Leu Asn 370 taaggatcag cgaataaaat tgg <210> 414 <211> 381 <212> PRT <213> Corynebacteriun glutainicum <400> 414 Met Glu Pro Val Tyr Val Lys Arg Arg Gin Arg Phe Ile Ala Val Thr 609 WO 01/00843 1 Ile Ala Se Ala Thr SE Val Vai GJ Giy Pro Gl Gin Thr A] Phe Tyr Ai Leu Leu Ac Aia Thr Le 130 Ile Tyr S( 145 Cys Ile T1 Aia Giu LE Giy Thr A~ Val Vai A~ 210 Thr Arg SE 225 Asp Ala I Ile Giu A3 Ile Leu A~ Vai Asn 7) 290 Arg Gin TI 305 Gin Thr PJ ~r Leu ~r Asn .n Leu .u Leu .a Ala :g Leu 100 ;p Pro *u Met *r Gin *r Ala u Giy 180 ;p Pro ;p Pro ar Ala le Gly ~g Giu 260 sn Arg 'r Giy ,ir Vai ro Ile 5 Ile Arg Val Giu Ser Gin Asp Asp Ile Giu 165 Val Lys Ser Asn Leu 245 Aia Leu Leu Thr Aia 325 Leu Thr Giu Giu 70 Asn Giu Asn Val Ala 150 Asp Pro Arg Asn His 230 Thr Pro Aia Ser Pro 310 Ala Ile Pro Ile Arg Asn Gin Gin Thr i3 Ala Leu Asp Leu Asp 215 Phe Pro Ala Giu Giu 295 Trp Val Ile His Pro Asp Pro Met Val 120 Val Val1 Gin Trp Giu 200 Ala Gin Tyr Gly Pro 280 Gin Asn Ser Giy 25 Asp Giu Ile Asn Asn 105 Asp Val1 Thr Gin Ala 185 G iy Gin Giu Giu.

Asp 265 Met Giu Thr Thr Ala Tyr Gly Val Ala 90 Ala Leu Gly Cys Val 170 Ile Leu Giy Thr Leu 250 Phe Gin Val Tyr Giu 330 le Giu Ser Ala 75 G iy Ala Leu Gly Thr i55 Ala Ala Ile Ile Asp 235 Val1 Asp Leu Al a Ala 315 Ala Ile Giy Ser Ser Gin Vai 110 His Arg Ser Vai Giu 190 Gly Asp Gly Ala Aia 270 Asp Asp Gly Ala PCTIBOO/00923 Gly Val Asn Gly Giu Leu Ala Phe Pro Gly Ser Ala Gly Gly Ala Gly Ala Asn 160 Ser Pro 175 Ala Arg Gin Tyr Leu Ile Arg Ala 240 Ser Leu 255 Arg Vai Ser Thr Glu Asp Leu Pro *320 Met Giu 335 WO 01/00843 WO 0100843PCT/IBOO/00923 Asn Pro Ala Giu Gly Asn Trp, Leu Tyr 340 345 Gly Thr Thr Val Phe Asn Asp Thr Phe 355 360 Phe Val Thr Ile Asp Thr Asp 350 Ala Asp Ile Glu Giu His Giu Gin Ala Leu Asn Ser Gly Val Leu Asp Ser Asn Arg 370 375 380 415 <211> 644 <212> DNA <213> Corynebacteriun glutamicum <2 <221> CDS <222> <223> RXN00954 <400> 415 gct gtg a~ Aia Val L: 1 ag tgg ttc vs Trp Phe 5 gaa gcg tcc aac Giu Ala Ser Asn acc ttc ctg ttc gca cct Thr Phe Leu Phe Ala Pro gcg tac aac Ala Tyr Asn aaa ttc ccc Lys Phe Pro gcg att gcg cat Ala Ile Ala His cag ccg gtt cgc Gin Pro Val Arg cag gcg ctg Gin Ala Leu tcc ccg gcg Ser Pro Ala acc atc ttc aac Thr Ile Phe Asn ctt gga cca ttg Leu Giy Pro Leu cgc ccg Arg Pro gag cgt cag atc atg ggc gtg gcc aat Glu Arg Gin Ilie Met Gly Val Ala Asn 55 gcc Ala aat cat gga cag Asn His Gly Gin C tC Leu atc gcc gag gtc Ile Ala Glu Val cgc gag ttg ggc Arg Glu Leu Gly aca cgc gcg ctt Thr Arg Ala Leu gtg cat ggc gca Val His Gly Ala ggc Gly acc gat gag atc Thr Asp Giu Ile gtc cac ggc acc Val His Gly Thr acc ttg Thr Leu gtg tgg gag Val Trp Giu cct gag gac Pro Giu Asp 115 aaa gaa gac ggc Lys Glu Asp Gly atc gag cat tac Ile Giu His Tyr acc atc gag Thr Ile Giu 110 ctc gta ggt Leu Vai Gly ctt ggc ctt ggc Leu Gly Leu Gly tac acc ctt gag Tyr Thr Leu Giu gat Asp 125 ggc ctc Gly Leu 130 ggc act gag aac Gly Thr Glu Asn gaa gct atg cgc Glu Ala Met Arg act ttc gcg ggc Thr Phe Ala Gly acc ggc cct gat gca Thr Giy Pro Asp Ala 145 cac His 150 cgt gat gcg ttg Arg Asp Ala Leu gcg tcc gca ggt Ala Ser Ala Gly WO 01/00843 WO 0100843PCTILBOO/00923 atg ttc tac ctc aac ggc gat gtc gac tcc ttg aaa gat ggt gca caa Met Phe Tyr Leu Asn Gly Asp Val Asp Ser Leu Lys Asp Gly Ala Gin 165 170 175 aag gcg ctt tcc ttg ctt gcc gac ggc acc acc cag gca tgg ttg gcc Lys Ala Leu Ser Leu Leu Ala Asp Gly Thr Thr Gin Ala Trp Leu Ala 180 185 190 aag cac gaa gag atc gat tac tca gaa aag gag tct tcc aat gac Lys His Glu Glu Ilie Asp Tyr Ser Giu Lys Giu Ser Ser Asn Asp 195 200 205 tagtaataat ctgcccacag tgt <210> 416 <211> 207 <212> PRT <213> Corynebacterium glutamicum <400> 416 Ala Val Lys Trp Giu Ala Ser Asn 1 Ala Lys Arg Leu Val Val1 Pro Gly Thr 145 Met Lys Lys Tyr Phe Pro Ilie His Trp Glu Leu 130 Giy Phe Ala His Asn Pro Giu Al a Gly Giu Asp 115 Giy Pro Tyr Leu Giu 195 Phe Gin Gly Ala Gly Ala Ile Thr Met Leu Ser 170 Thr Lys Thr Pro Pro Asn Arg Val Glu Leu Arg Al a 155 Leu Thr Glu Phe Val Leu Ala Thr His His Glu Ala 140 Ala Lys Gin Ser Leu Arg Leu Asn Arg Gly Tyr Asp 125 Thr Ser Asp Al a Ser 205 WO 01/00843 WO 0100843PCTIIBOO/00923 <210> 417 <211> 611 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (588) <223> FRXA00954 <400> 417 ttc ctg t Phe Leu P.

1 tc gca cct he Ala Pro 5 gcg tac aac cct gcg att gcg cat gtg Ala Tyr Asn Pro Ala Ile Ala His Val 10 cag ccg Gin Pro gtt cgc cag Val Arg Gin ttg ctg tcc Leu Leu Ser ctg aaa ttc ccc Leu Lys Phe Pro atc ttc aac acg Ile Phe Asn Thr ctt gga cca Leu Gly Pro gtg gcc aat Val Ala Asn ccg gcg cgc ccg gag cgt cag atc atg Pro Ala Arg Pro Giu Arg Gin Ile Met ggc Gly gcc aat Ala Asn cat gga cag ctc His Gly Gin Leu gcc gag gtc ttc Ala Glu Val Phe gag ttg ggc cgt Giu Leu Gly Arg cgc gcg ctt gtt Arg Ala Leu Val gtg Val cat ggc gca ggc His Gly Ala Gly gat gag atc gca Asp Giu Ile Ala cac ggc acc acc His Gly Thr Thr t tg Leu gtg tgg gag ctt Val Trp Giu Leu gaa gac ggc acc Giu Asp Gly Thr atc gag Ile Giu cat tac acc His Tyr Thr gag gat ctc Giu Asp Leu 115 gag cct gag gac Giu Pro Glu Asp ggc ctt ggc cgc Gly Leu Gly Arg tac acc ctt Tyr Thr Leu 110 gct atg cgc Aia Met Arg gta ggt ggc ctc Val Giy Gly Leu act gag aac gcc Thr Giu Asn Ala gaa Giu 125 gct act Ala Thr 130 ttc gcg ggc acc Phe Ala Gly Thr cct gat gca cac Pro Asp Ala His cgt Arg 140 gat gcg ttg gct Asp Ala Leu Ala tcc gca ggt gcg Ser Ala Gly Ala ttc tac ctc aac Phe Tyr Leu Asn ggC Gly 155 gat gtc gac tcc Asp Val Asp Ser t tg Leu 160 aaa gat ggt gca Lys Asp Gly Ala caa Gin 165 aag gcg ctt tcc Lys Ala Leu Ser ctt gcc gac ggc Leu Ala Asp Gly acc acc Thr Thr 175 cag gca tgg Gin Ala Trp gcc aag cac gaa Ala Lys His Giu atc gat tac tca Ile Asp Tyr Ser gaa aag gag GluLys Giu 190 tct tcc aat gac tagtaataat ctgcccacag tgt WO 01/00843 PCTIABOO/00923 Ser Ser Asn Asp 195 <210> 418 <211> 196 <212> PRT <213> Corynebacterium giutamicum <400> 418 Phe Leu Phe Ala Pro Ala Tyr Asn Pro Ala Ile Ala His Val Gln Pro 1 5 10 Val Arg Gin Ala Leu Lys Phe Pro Thr Ile Phe Asn Thr Leu Gly Pro 25 Leu Leu Ser Pro Ala Arg Pro Giu Arg Gin Ile Met Giy Val Ala Asn 40 Ala Asn His Gly Gin Leu Ile Ala Glu Val Phe Arg Glu Leu Gly Arg 55 Thr Arg Ala Leu Val Val His Gly Ala Gly Thi- Asp Giu Ile Ala Val 70 75 His Gly Thr.Thr Leu Val Ti-p Giu Leu Lys Glu Asp Gly Th- Ile Glu 90 His Tyr Thr Ile Giu Pro Glu Asp Leu Gly.Leu Gly Arg Tyr Thr Leu 100 105 110 Giu Asp Leu Val Gly Gly Leu Gly Thr Glu Asn Ala Giu Ala Met Arg 115 120 125 Ala Thi- Phe Ala Gly Thr Gly Pro Asp Ala His Arg Asp Ala Leu Ala 130 135 140 Ala Ser Ala Gly Ala Met Phe Tyr Leu Asn Gly Asp Val Asp Ser Leu 145 150 155 160 Lys Asp Gly Ala Gin Lys Ala Leu Ser Leu Leu Ala Asp Gly Thr Thr 165 170 175 Gin Ala Ti-p Leu Ala Lys His Giu Glu Ile Asp Tyr Ser Giu Lys Glu 180 185 190 Ser Ser Asn Asp 195 <210> 419 <211> 1677 <212> DNA <213> Corynebacteriun giutaxnicum <22 0> <221> CDS <222> (101)..(1654) <223> RXN00957 <400> 419 WO 01/00843 WO 0100843PCTAIBOO/00923 aaccggcaag ccctggatcg aatgaagctc gcagcgagta attatttgat gtttcccaga aaggcttcag ccccacaatg atttcctcgg taggtgcccc atg agc acg aat Met Ser Thr Asn 1 cat gtt ttc tcc His Val Phe Ser gat gtc cgc tat Asp Val Arg Tyr gag gat gct tct Giu Asp Ala Ser gca ttg Ala Leu ttt gcc cac Phe Ala His agc gct gat Ser Ala Asp ggt ggc aca acc Gly Gly Thr Thr gat gat gca gcc Asp Asp Ala Ala ctg ttg gaa Leu Leu Glu gcg gtg ttg Ala Val Leu atc acc acc aag Ile Thr Thr Lys ggt att tct tcc Gly Ilie Ser Ser ctc Leu aag agt Lys Ser tcg gtg cgc att Ser Val Arg Ile tgc acg ggc aac Cys Thr Gly Asn acg Thr gtg gta acg cag Val Val Thr Gin ctg acg gac tcg Leu Thr Asp Ser ggt Gly agg gca gtg gtt Arg Ala Val Val cgc cta acg cag Arg Leu Thr Gin ctt ggc cag tac Leu Gly Gin Tyr acc gca gag aac Thr Ala Giu Asn ttt agc ttc ccc Phe Ser Phe Pro gcc tca Ala Ser 100 gat gcg gtt Asp Ala Val gtg ctg cgc Val Leu Arg 120 gag cgc gag cgc Giu Arg Giu Arg acc gca cca. agc Thr Ala Pro Ser acc atc gaa Thr Ile Glu 115 gcg tcc ctg Ala Ser Leu aag ttg cag ttc Lys Leu Gin Phe tcc ggt tac agc Ser Gly Tyr Ser gac Asp 130 cca ctg Pro Leu 135 ctc atg ggc ggt Leu Met Gly Gly t tc Phe 140 gcg ttt gat ttc Ala Phe Asp Phe gaa acc ttt gaa Giu Thr Phe Giu acg Thr 150 ctc ccc gct gtc Leu Pro Ala Val gag Giu 155 gag agc gtc aac Glu Ser Val Asn tac ccc gat tac Tyr Pro Asp Tyr ttc gtc ctc gcg Phe Val Leu Ala gaa Giu 170 atc gtc ctg gac Ile Val Leu Asp aat cac cag gac Asn His Gin Asp cag acc Gin Thr 180 gcc aaa ctc Ala Lys Leu ctc aac aag Leu Asn Lys 200 ggc gtc tcc aac Gly Val Ser Asn cca ggc gag ctc Pro Gly Giu Leu gag gcc gag Giu Ala Glu 195 gca acc gaa Ala Thr Giu ctt tca ttg ctt Leu Ser Leu Leu gac gcc gcc ctc Asp Ala Ala Leu cac gcc His Ala 215 tac caa acc acc Tyr Gin Thr Thr cc t Pro 220 cac gac ggc gac His Asp Gly Asp ctt cgc gtt gtg Leu Arg Val Val WO 01/00843 gct gat att Ala Asp Ile 230 gaa aac at~t Glu Asn Ile PCTIBOOIOO923 ccc gat gct cag ttc cgc acc Pro Asp Ala Gin Phe Arg Thr atc aat gag ctg Ile Asn Glu Leu aaa Lys 245 tac Tyr aac Asn 250 tg t Cys gac atc tac Asp Ilie Tyr gtc ccg gcg Val Pro Ala cgc act Arg Thr 260 835 883 931 979 ttc acc gca Phe Thr Ala gcc acc aac Ala Thr Asn 280 ggc cgc tcc 1027 Gly Arg Ser 295 ttc acc gct 1075 Phe Thr Ala 310 cgc ccc cgt 1123 Arg Pro Arg cca Pro 265 ccg Pro cct gat gca Pro Asp Ala gct tat ctg Ala Tyr Leu cag ctg cgt Gin Leu Arg 275 tcg ccg tac Ser Pro Tyr tat gaa ctt ttt Tyr Glu Leu Phe 300 gct aac cgt gag Ala Asn Arg Giu 315 atg ttc Met Phe 285 ggc gca Gly Ala ctg cag Leu Gin tat atc cgt Tyr Ilie Arg tcc cct gag Ser Pro Glu 305 ctg tac cca Leu Tyr Pro ggc ctc aac gaa Gly Leu Asn Glu 290 tcc aac ctc aag S er Asn Leu Lys atc gca ggt acc Ile Ala Gly Thr 325 320 gga ctc Gly Leu 330 atc cgc 1171 Ile Arg cac acc 1219 His Thr gtc cca 1267 Val Pro 375 tcc cgc 1315 Ser Arg 390 gag ctt 1363 Glu Leu ttg acc 1411 Leu Thr aat gag Asn Glu 345 atg ctt Met Leu ttg Leu gtc Val aac cca gat ggc tcc Asn Pro Asp Gly Ser 335 gat atg cgc act gat Asp Met Arg Thr Asp 350 gat ctc gcc cgc aac Asp Leu Ala Arg Asn atc Ile aac gat gag cta gat Asn Asp Glu Leu Asp 340 gcc aaa gag atc gcg gag Ala Lys Glu Ile Ala Glu 355 gac ctg gcc cgc gtc tcg Asp Leu Ala Arg Val Ser 360 365 gcg tcg cgc cgg gtt Ala Ser Arg Arg Val 380 gtg atg cac ttg gtg Val Met His Leu Vai 395 gat gct ttg gac gcc Asp Ala Leu Asp Ala 410 ggc gct ccg aag ttg Gly Ala Pro Lys Leu 425 gcg gat ctt ttg cag Ala Asp Leu Leu Gin 385 tcc cgt gtg acg gcg Ser Arg Val Thr Ala 400 tat cgg gcg tgc atg Tyr Arg Ala Cys Met 370 gtg gat cgc tat Vai Asp Arg Tyr acg ttg gac cca Thr Leu Asp Pro 405 aat atg ggc acg Asn.Met Gly Thr 415 cgc gct atg Arg Ala Met 430 gag ctg ttg cgc Glu Leu Leu Arg 435 420 ggc gtc Gly Val WO 01/00843 WO 0100843PCTIiBOO/00923 gaa aag cgc agg cgt ggt tct tat ggt ggg gca gtg ggg tac ctg cgc 1459 Giu Lys Arg Arg Arg Giy Ser Tyr Gly Gly Ala Val Gly Tyr Leu Arg 440 445 450 ggc aat ggc gat atg gat aat tgc att gtt att cgt tcg gcg ttt gtc 1507 Gly Asn Giy Asp Met Asp Asn Cys Ile Val Ile Arg Ser Ala Phe Val 455 460 465 cag gat ggt gtg gct gct gtg cag gct ggt gct ggt gtg gtc cgc gat 1555 Gin Asp Gly Val Ala Ala Val Gin Ala Gly Ala Giy Val Val Arg Asp 470 475 480 485 tct aat cct caa tct gaa gcc gat gag acg ttg cac aag gcg tat gcc 1603 Ser Asn Pro Gin Ser Giu Ala Asp GlU Thr Leu His Lys Ala Tyr Ala 490 495 500 gtg ttg aat gcc att gcg ctt gct gct ggt tcc act ttg gag gtc atc 1651 Val Leu Asn Ala Ile Ala Leu Ala Ala Gly Ser Thr Leu Giu Vai Ile 505 510 515 cga tgacacacgt tgttctcatt gat 1677 Arg <210> 420 <211> 518 <212> PRT <213> Corynebacterium glutamicum <400> 420 Met Ser Thr Asn Pro His Val Phe Ser Leu Asp Val Arg Tyr His Giu 1 5 10 Asp Ala Ser Ala Leu Phe Ala His Leu Gly Gly Thr Thr Ala Asp Asp 25 Ala Ala Leu Leu Glu Ser Ala Asp Ile Thr Thr Lys Asn Gly Ile Ser 40 Ser Leu Ala Val Leu Lys Ser Ser Val Arg Ile Thr Cys Thr Gly Asn 55 Thr Val Val Thr Gin Pro Leu Thr Asp Ser Giy Arg Ala Vai Val Ala 70 75 Arg Leu Thr Gin Gln Leu Gly Gin Tyr Asn Thr Ala Glu Asn Thr Phe 90 Ser Phe Pro Ala Ser Asp Ala Val Asp Glu Arg Giu Arg Leu Thr Ala 100 105 110 Pro Ser Thr Ile Glu Val Leu Arg Lys Leu Gin Phe Glu Ser Gly Tyr 115 120 125 WO 01/00843 PT10102 PCT/EBOO/00923 Ser Leu Pro Leu Leu Met Gly Gly Phe Ala Phe Asp Phe 135 140 Phe Tyr Gin 180 Ala Thr Val Leu Arg 260 Leu Asn Leu Gly Leu 340 Ala Val Arg Asp G ly 420 Gly Giu Gin 165 Thr Giu Glu Val1 Lys 245 Thr Arg Glu Lys Thr 325 Asp Glu Ser Tyr Pro 405 Thr Val Thr 150 Phe Ala Leu His Ala 230 Glu Phe Al a G ly Phe 310 Arg Ile His Val1 Ser 390 Giu Leu Glu Leu Val Lys Asn Ala 215 Asp Asn Thr Thr Arg 295 Thr Pro Arg Thr Pro 375 Arg Leu Thr Lys Giu Giu 155 Ile Val Val Ser Leu Leu Thr Pro 220 Ala Gin 235 Gly Asp Pro Asp Pro Tyr Leu Phe 300 Arg Giu 315 Asn Pro Asp Met Asp Leu Arg Val 380 Leu Val 395 Asp Ala Lys Leu Gly Ser Ser Leu Asn Ile 205 His Phe Ile Al a Met 285 Gly Leu Asp Arg Ala 365 Ala Ser Tyr Arg Tyr 445 Asn I le 175 Pro Ala Gly Thr Gln 255 Ala Tyr Ser Leu Ser 335 Asp Asn Leu Val1 Ala 415 Met Gly Thr 160 Asn Gly Ala Asp Gin 240 Val1 Ala Ile Pro Tyr 320 Ile Ala Asp Leu Thr 400 Cys Giu Ala WO 01/00843 Val Gly T 450 Arg Ser A] 465 Gly Val Ve His Lys A] Thr Leu GI Leu Arg Phe Val Arg Asp 485 Tyr Ala 500 Val Ile PCT/iBOO/00923 Gly Asn Gly Asp Met Asp Asn Cys Ile Val Ile 455 460 Gin Asp Gly Val Ala Ala Val Gin Ala Gly Ala 470 475 480 Ser Asn Pro Gin Ser Giu Ala Asp Giu Thr Leu 490 495 Val Leu Asn Ala Ile Ala Leu Ala Ala Gly Ser 505 510 Arg <210> 421 <211> 1151 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (1128) <223> FRXA00957 <400> 421 gat ttc tta gaa a Asp Phe Leu Giu T 1 aac act tac ccc g Asn Thr Tyr Pro A atc aat cac cag g Ilie Asn His Gin A cca ggc gag ctc g Pro Gly Giu Leu G gcc gcc ctc ccc g Ala Ala Leu Pro A ggc gac act ctt c Gly Asp Thr Leu A acc cag atc aat g Thr Gin Ilie Asn G 100 caa gtt gtc ccg g Gin Val Val Pro A 115 cc ttt gaa hr Phe Giu 5 at tac cag .sp Tyr Gin ac cag acc .sp Gin Thr ag gcc gag iu Ala Giu ca acc gaa la Thr Giu gc gtt gtg .rg Val Val ag ctg aaa iu Leu Lys cg cgc act la Arg Thr acg ctc ccc Thr Leu Pro 10 gtc gag gag agc gtc Val Giu Giu'Ser Val gtc Val 25 aaa Lys aac Asn gcc Ala ga t Asp aac Asn 105 ac c Thr ctc Leu ctc Leu aag Lys tac Tyr att Ilie 90 att Ile gca Ala aac atc Ile gtc Val t tg Leu acc Thr gc t Ala ggt Gly cct Pro 125 ccg Pro gtc Vai tcc Ser ctt Leu cc t Pro cag Gin gac Asp 110 gat Asp tac Tyr ctg Leu aac Asn atc Ile cac His ttc Phe atc Ile gca Ala atg Met gct gct tat ctg cag ctg cgt gcc acc Ala Ala Tyr Leu Gip Leu Arg Ala Thr Asn Pro Ser WO 01/00843 PCTJIBOO/00923 130 135 140 tat atc cgt ggc ctc aac gaa ggc cgc tcc tat gaa ctt ttt ggc gca 480 Tyr Ilie Arg Gly Leu Asn Giu Gly Arg Ser Tyr Glu Leu Phe Gly Ala 145 150 155 *160 tcc cct gag tcc aac ctc aag ttc acc gct gct aac cgt gag ctg cag 528 Ser Pro Giu Ser Asn Leu Lys Phe Thr Ala Ala Asn Arg Giu Leu Gin 165 170 175 ctg tac cca atc gca ggt acc cgc ccc cgt gga ctc aac cca gat ggc 576 Leu Tyr Pro Ile Ala Gly Thr Arg Pro Arg Gly Leu Asn Pro Asp Gly 180 185 190 tcc atc aac gat gag cta gat atc cgc aat gag ttg gat atg cgc act 624 Ser Ile Asn Asp Giu Leu Asp Ilie Arg Asn Giu Leu Asp Met Arg Thr 195 200 205 gat gcc aaa gag atc gcg gag cac acc atg ctt gtc gat ctc gcc cgc 672 Asp Ala Lys Giu Ilie Ala Giu His Thr Met Leu Val Asp Leu Ala Arg 210 215 220 aac gac ctg gcc cgc gtc tcg gtc cca gcg tcg cgc cgg gtt gcg gat 720 Asn Asp Leu Ala Arg Val Ser Val Pro Ala Ser Arg Arg Val Ala Asp 225 230 235 240 ctt ttg cag gtg gat cgc tat tcc cgc gtg atg cac ttg gtg tcc cgt 768 Leu Leu Gin Val Asp Arg Tyr Ser Arg Val Met Hius Leu Val Ser Arg 245 250 255 gtg acg gcg acg ttg gac cca gag ctt gat gct ttg gac gcc tat cgg 816 Val Thr Ala Thr Leu Asp Pro Giu Leu Asp Ala Leu Asp Ala Tyr Arg 260 265 270 gcg tgc atg aat atg ggc acg ttg acc ggc gct ccg aag ttg cgc gct 864 Ala Cys Met Asn Met Gly Thr Leu Thr Gly Ala Pro Lys Leu Arg Ala 275 280 285 atg gag ctg ttg cgc ggc gtc gaa aag cgc agg cgt ggt tct tat ggt 912 Met Giu Leu Leu Arg Gly Val Giu Lys Arg Arg Arg Gly Ser Tyr Gly 290 295 300 ggg gca gtg ggg tac ctg cgc ggc aat ggc gat atg gat aat tgc att 960 Gly Ala Val Gly Tyr Leu Arg Gly Asn Gly Asp Met Asp Asn Cys Ile 305 310 315 320 gtt att cgt tcg gcg ttt gtc cag gat ggt gtg gct gct gtg cag gct 1008 Val Ilie Arg Ser Ala Phe Val Gin Asp Gly Val Ala Ala Val Gin Ala 325 330 335 ggt gct ggt gtg gtc cgc gat tct aat cct caa tct gaa gcc gat gag 1056 Gly Ala Gly Val Val Arg Asp Ser Asn Pro Gin Ser Giu Ala Asp Giu 340 345 350 acg ttg cac aag gcg tat gcc gtg ttg aat gcc att gcg ctt gct gct 1104 Thr Leu His Lys Ala Tyr Ala Val Leu Asn Ala Ile Ala Leu Ala Ala 355 360 365 WO 01/00843 PCTIIBOO/00923 ggt tcC act ttg gag gtc atc cga tgacacacgt tgttctcatt gat 11521 Gly Ser Thr Leu Glu Val Ile Arg 370 375 <210> 422 <211> 376 <212> PRT <213> Corynebacteriun glutaxnicum <400> 422 Asp Phe Leu Giu Thr Phe Giu Thr Leu Pro Ala Val Giu Glu Ser Val 1 5 10 Asn Thr Tyr Pro Asp Tyr Gin Phe Val Leu Ala Glu Ile Val Leu Asp 25 Ile Asn His Gin Asp Gin Thr Ala Lys Leu Ala Gly Val Ser Asn Ala 40 Pro Gly Giu Leu Giu Ala Glu Leu Asn Lys Leu Ser Leu Leu Ile Asp 55 Ala Ala Leu Pro Ala Thr Giu His Ala Tyr Gln Thr Thr Pro His Asp 70 75 Gly Asp Thr Leu Arg Val Val Ala Asp Ilie Pro Asp Ala Gin Phe Arg 90 Thr Gin Ilie Asn Giu Leu Lys Glu Asn Ile Tyr Asn Gly Asp Ile Tyr 100 105 110 Gin Val Val Pro Ala Arg Thr Phe Thr Ala Pro Cys Pro Asp Ala Phe 115 120 125 Ala Ala Tyr Leu Gin Leu Arg Ala Thr Asn Pro Ser Pro Tyr Met Phe 130 135 140 Tyr Ilie Arg Gly Leu Asn Glu Gly Arg Ser Tyr Giu Leu Phe Gly Ala 145 150 155 160 Ser Pro Glu Ser Asn Leu Lys Phe Thr Ala Ala Asn Arg Giu Leu Gin 165 170 175 Leu Tyr Pro Ile Ala Gly Thr Arg Pro Arg Giy Leu Asn Pro Asp Gly 180 185 190 Ser Ilie Asn Asp Glu Leu Asp Ile Arg Asn Glu Leu Asp Met Arg Thr 195 200 205 Asp Ala Lys Glu Ile Ala Glu His Thr Met Leu Val Asp Leu Ala Arg 210 215 220 Asn Asp Leu Ala Arg Val Ser Val Pro Ala Ser Arg Arg Val Ala Asp 225 230 235 240 Leu Leu Gin Val Asp Arg Tyr Ser Arg Val Met His Leu Val Ser Arg 245 250 255 Val Thr Ala Thr Leu Asp Pro Glu Leu Asp Ala Leu Asp Ala Tyr Arg WO 01/00843 WO 0100843PCT/IBOO/00923 260 265 Ala Cys Met Asn Met Gly Thr Leu Thr Gly Ala 275 280 Met Glu Leu Leu Arg Gly Val GlU Lys Arg Arg 290 295 Gly Ala Val Gly Tyr Leu Arg Giy Asn Gly Asp 305 310 315 Val Ilie Arg Ser Ala Phe Val Gin Asp Gly Val 325 330 Gly Ala Gly Val Val Arg Asp Ser Asn Pro Gin 340 345 Thr Leu His Lys Ala Tyr Ala Val Leu Asn Ala 355 360 Gly Ser Thr Leu Giu Val Ile Arg 370 375 <210> 423 <211> 1068 <212> DNA <213> Corynebacterium giutanicum <220> <221> CDS <222> (101)..(1045) <223> RX.A02687 <400> 423 caaaaccatg ctcggcgggg gagagttggg ccaagcggtt tggacaccca ccttagttcg gcgggttaag ctgtgtaacc Arg Tyr Cys Gin 335 Asp Ala act Thr C tc Leu cag Gin Gly ccc Pro cgctaattga gcaacagaca atg agc gac gca cca Met Ser Asp Ala Pro 1 ttc acc gaa gaa gcc Phe Thr Giu Giu Ala gac ggt gag atc gag Asp Gly Giu Ile Giu gac gcc gtc cgc cac Asp Ala Val Arg His aac ttc gtc gac ggc Asn Phe Val Asp Gly ggc tcc aac: gtg caa Gly Ser Asn Val Gin cta cca Leu Pro acc gcc Thr Ala gtc acc Val Tbr WO 01/00843 WO 0100843PCT/iBOO/00923 atc atc Ile Ile cca ggg Pro Gly ggg tac Giy Tyr atg tat Met Tyr 135 gaa gga Giu Giy 150 ttc gga Phe Gly cgc acc Arg Thr acc ggc Thr Gly ggc agc Gly Ser 215 ctc acc Leu Thr 230 cgc tic Arg Phe gaa gcc Giu Ala ggc tcc Gly Ser caa cta 1027 Gin Leu 295 gaa gga 1068 gcc gaa gaa Ala Giu Giu act tcg ct Thr Ser Leu 105 caa caa gig Gin Gin Val 120 cit ica gca Leu Ser Ala acc gcc gac Thr Ala Asp cig gaa cgc Leu Giu Arg 170 cgc tic gt Arg Phe Val 185 cac gac cgc His Asp Arg 200 cic gig cgc Leu Val Arg cgc aic gaa Arg Ilie Glu cac cig gac His Leu Asp 250 cic cgc gca Leu Arg Ala 265 igg ccc icc Trp, Pro Ser 280 gct aag cia Ala Lys Leu agg aaa ct gaa cic gac aic gcc iii icc Giu Leu Asp Ile Ala Phe Ser aic atg gtc cgg Ile Met Val Arg 95 .100 gcc gac Ala Asp aaa aac Lys Asn agc icc Ser Ser 140 gca gcc Ala Ala 155 cti gt Leu Val gci gic Ala Val acc icc Thr Ser gcc cic Ala Leu 220 icc cgc Ser Arg 235 ata icc Ile Ser cic cac Leu His aac cgi Asn Arg cac aag His Lys 300 aac tag gic Val tgg Trp 125 aac Asn gca Ala gat Asp caa Gin gic Val1 205 aac Asn ccc Pro gga Gly c ic Leu gcg Ala 285 gcg aaa Lys 110 atg Met ggc Gly gcg Ala ga t Asp gcc Ala 190 att Ile gaa Giu acc Thr cat His c aa Gin 270 gaa Giu gac acc Thr gca Ala gcc Ala ccc Pro gtc Val1 175 caa Gin ttc Phe tc Phe cgc Arg aic Ile 255 gcc Ala gac Asp gaa c ic Leu acc Thr ggc Gly tcc Ser 160 gcc Ala gca Ala icc Ser gcc Ala aaa Lys 240 cgc Arg gaa Glu agc Ser igg gcc acc Ala Thr acc at Thr Ile 130 gca caa Ala Gin 145 cgc gca Arg Ala gac gtc Asp Val gcc git Ala Val cta ccg Leu Pro 210 aic cgc Ile Arg 225 gtc tic Vai Phe gat aic Asp Ile gaa cic Giu Leu acg ccc Thr Pro 290 gii cgc Val Arg 305 403 451 499 547 595 643 691 739 787 835 883 931 979 Ala Asp Glu Trp ccatggc cggccggait at Giu Gly Arg Lys Leu Asn 310 315 WO 01/00843 WO 0100843PCTLBOOIOO923 <210> 424 <211> 315 <212> PRT <213> Corynebacteriun glutamicum <400> 424 Met Ser Asp Ala Pro Thr Val Val Ala Tyr Leu Gly Pro Ala Gly Thr 1 5 10 Phe Thr Giu Giu Ala Leu Tyr Lys Phe Ala Asp Ala Gly Vai Phe Gly 25 Asp Gly Glu Ile Giu Gin Leu Pro Ala Lys Ser Pro.Gin Giu Ala Val 40 Asp Ala Val Arg His Gly Thr Ala Gin Phe Ala Val Val Ala Ile Glu 55 Asn Phe Val Asp Gly Pro Val Thr Pro Thr Phe Asp Ala Leu Asp Gin 70 75 Gly Ser Asn Val Gin Ile Ile Ala Giu Giu Glu Leu Asp Ile Ala Phe 90 Ser Ile Met Val Arg Pro Gly Thr Ser Leu Ala Asp Val Lys Thr Leu 100 105 110 Ala Thr His Pro Vai Gly Tyr Gin Gin Val Lys Asn Trp, Met Ala Thr 115 120 125 Thr Ile Pro Asp Ala Met Tyr Leu Ser Ala Ser Ser Asn Gly Ala Gly 130 135 140 Ala Gin Met Val Ala Giu Gly Thr Ala Asp Ala Ala Ala Ala Pro Ser 145 150 155 160 Arg Ala Ala Glu Leu Phe Gly Leu Glu Arg Leu Val Asp Asp Val Ala 165 170 175 Asp Val Arg Gly Ala Arg Thr Arg Phe Val Aia Val Gin Ala Gin Ala 180 185 190 Ala Val Ser Glu Pro Thr Gly His Asp Arg Thr Ser Val Ile Phe Ser 195 200 205 Leu Pro Asn Val Pro Gly Ser Leu Val Arg Ala Leu Asn Glu Phe Ala 210 215 220 Ile Arg Gly Val Asp Leu Thr Arg Ile Glu. Ser Arg Pro Thr Arg Lys 225 230 235 240 Val Phe Gly Thr Tyr Arg Phe His Leu Asp Ile Ser Gly His Ilie Arg 245 250 255 Asp Ile Pro Val Ala Glu Ala Leu Arg Ala Leu His Leu Gin Ala Giu 260 265 270 Giu Leu Val Phe Val Gly Ser Trp Pro Ser Asn Arg Ala Glu Asp Ser 275 280 285 WO 01/00843 WO 0100843PCT/BOOIOO923 Thr Pro Gin Thr Asp Gin Leu Ala Lys Leu His Lys Ala Asp Giu Trp, 290 295 300 Val Arg Ala Ala Ser Giu Gly Arg Lys Leu Asn 305 310 315 <210> 425 <211> 1353 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(1330) <223> RXN01698 <400> 425 cgctagtgcc tgcagttatg tcgctttttg acgtcgaaaa gcaattttaa aaagcccttg gggtgggctg gatacgtctt ctgcggcctt tcgtgcgata atg cta ggc atg Met Leu Gly Met 1 cga tgg act aca Arg Trp Thr Thr ggt gaa tcc cac Gly Glu Ser His cag gcg ctt atc Gin Ala Leu Ile gcc acg Ala Thr gtt gaa cac Val Glu His tat caa ttg Tyr Gin Leu atg Met cca gca ggc gtg Pro Ala Gly Val gtg act aaa gat Val Thr*Lys Asp gag gtc tcg Giu Val Ser gct cgc atg Ala Arg Met gcg cgc cga cgc Ala Arg Arg Arg gga tat ggt cgc Gly Tyr Gly Arg ggC Gly aag ttt Lys Phe gag caa gac gcg Giu Gin Asp Ala acc ttc ctc acc Thr Phe Leu Thr ggc Gly atc cgc cac ggc Ile Arg His Gly act ttg ggt agc Thr Leu Gly Ser atc tca atc atg Ile Ser Ile Met ggc aac act gag Gly Asn Thr Glu gat aag tgg acc Asp Lys Trp Thr atc atg tcc tct Ilie Met Ser Ser gct ttg gac atg Ala Leu Asp Met gaa gac Giu Asp 100 cca gat aac Pro Asp Asn gtt Val1 105 gcg gcg atg tct Ala.Ala Met Ser ggt cgg ggc gca Gly Arg Gly Ala aaa ctg act Lys Leu Thr 115 aag tac gga Lys Tyr Gly cgt ccg cgt cca ggc cac gct gat Arg Pro Arg Pro Gly His Ala Asp 120 125 tac gca ggc atg Tyr Ala Gly Met c tc Leu 130 ttc gat Phe Asp 135 gat gcc cgc aac Asp Ala Arg Asn ctg gag cgt tct Leu Giu Arg Ser gcc cgt gag acg Ala Arg Giu Thr WO 01/00843 gca gca cgc Ala Ala Arg 150 acc ttg ggc Thr Leu Gly gca acc gtt gcg Ala Thr Val Ala gag cct Glu Pro gat gat Asp Asp atc gcg Ilie Ala 215 atc gtg Ile Val 230 att tct Ilie Ser ggc att Gly Ilie gct cgt Ala Arg aac ggc 1027 Asn Gly 295 atg acc 1075 Met Thr 310 tct act 1123 Ser Thr gca gca 1171 Ala Ala gcc ggt 1219 ctt Leu gag Glu gca Ala gca Ala 220 gaa Glu ctc Leu ggc Gly gaa Glu acc Thr 300 c tg Leu c tg Leu c ag Gin gca tcc Ser cca Pro t tc Phe 205 aag Lys ggc Gly gat Asp gtg Val1 gcc Ala 285 aac Asn cgc Arg aaa Lys cgt Arg atg cac His acc Thr 190 ggt Gly aaa Lys ctg Leu gcg Ala gaa Glu 270 cac Hius cgt Arg gtt Val1 acc Thr tcc Ser 350 gtc tcc Ser tcc Ser gat Asp gct Al a ga t Asp 225 ggt Gly gca Ala gac Asp gtg Val1 ggc Gly 305 ggc Gly atg Met tgc Cys gtt PCTIBOO/00923 cgt gaa 595 Arg Glu 165 gcg tcc 643 Ala Ser 180 gca atc 691 Ala Ile tcc atg 739 Ser Met ggt ggc 787 Gly Gly tca cac 835 Ser His 245 ctc atg 883 Leu Met 260 gaa gaa 931 Glu Glu gat gac 979 Asp Asp ggc ggc Gly Gly cca att Pro Ile 325 ggc aag Gly Lys 340 cca gcc Pro Ala cgc gca Ala Gly Val Val Ala Glu Ala Met Val Thr Leu Val Leu Ala Arg Ala 360 365 370 WO 01/00843 gtc ctg ca 1267 Val Leu Gi 375 att gac ac 1315 Ile Asp Th 390 tta gag ga 1353 Leu Glu As g aaa n Lys c tac .r Tyr ttc Phe c tc Leu PCTIBOO/00923 ggc ggt gac tcc ctg agc gaa acc aag agc aac Giy Gly Asp Ser Leu Ser Giu Thr Lys Ser Asri 380 385 aaa aac att gag gaa cga atg aaa ttc gaa ggt Lys Asn Ile Giu Glu Arg Met Lys Phe Glu Gly 395 400 405 taatgaagtg aatgatcaaa ttc t gga gcg p Gly Ala 410 <210> 426 <211> 410 <212> PRT <213> Corynebacteriun giutamicum <400> 426 Met Leu Giy Met Leu Arg Tip Thr Thr 1 5 Ala Leu Ilie Ala Thr Vai Glu His Met Lys Asp Glu Val Ser Tyr Gin Leu Ala 40 Arg Gly Ala Arg Met Lys Phe Glu Gin 55 Gly Ile Arg His Gly Leu Thr Leu Gly 70 Gly Asn Thr Giu Trp Asp Lys Trp Thr Leu Asp Met Giu Asp Pro Asp Asn Vai 100 105 Gly Ala Lys Leu Thr Arg Pro Arg Pro 115 120 Met Leu Lys Tyr Gly Phe Asp Asp Ala 130 135 Ser Ala Arg Glu Thr Ala Ala Arg Vai 145 150 Ser Phe Leu Arg Glu Thr Leu Gly Val 165 Ser Ile Gly Ala Ser Giu Pro Tyr Thr 180 185 Asp Ile Gin Ala Ile Asp Asp Ser Pro 195 200 Ala Pro Arg Asp Se r Thr Ala Gly Arg Al a Giu 170 Gly Val1 Gly Ala Arg Ala Pro Ile Ala His Asn Ala 155 Val Ala Arg Glu G ly Arg Leu Ile Met Met Ala Val 140 Ala Leu Giu Ala Ser Val Leu Thr Ser Ser Ser Asp 125 Leu Thr Ser Pro Phe 205 WO 01/00843 Ala Glu G2 210 Asp Thr LE 225 Gly Leu GI Ala Ala A] Asp Gly P12 Val Phe LE 290 Gly Leu GI 305 Gly Met Lj Met Glu Asc Cys Ala Ve Val Leu A] 370 Glu Thr L 385 Met Lys P1 Ser G ly S er Leu 260 Glu Asp Gly Pro Gly 340 Pro Arg Ser Giu Met Gly His 245 Met Giu Asp Gly Ile 325 Lys Ala Ala Asn Gly 405 Ala 215 Val1 Ser Ile Arg Gly 295 Thr Thr Ala Gly Leu 375 Asp Glu Glu Glu Gly Gin Arg 280 Val Asn Val Thr Val 360 Gin Thr Asp Ala Ala 220 Val Giu 235 Arg. Leu Lys Gly Ser Glu Asn Thr 300 Thr Leu 315 Ala Leu His Gin Glu Ala Gly Gly 380 Lys Asn 395 Lys Gly Asp Val Ala 285 Asn Arg Lys Arg Met 365 Asp Ile Lys Leu Ala Glu 270 His Arg Val Thr Ser 350 Val Ser Glu PCT/IBOO/00923 Ala Gly Pro Ile 240 Gin Ile 255 Ile Gly Asp Glu Ala Gly Arg Ala 320 Ilie Asp 335 Asp Val Thr Leu Leu Ser Giu Arg 400 <210> 427 <211> 1013 <212> DNA <213> Corynebacterium giutanicun <220> <221> CDS <222> (990) <223> FRXA01698 <400> 427 ggc aac act gag tgg gat aag tgg acc acc atc atg tcc tct gac gct Gly Asn Thr Glu Trp Asp Lys Trp Thr Thr Ilie Met Ser Ser Asp Ala 1 5 10 ttg gac atg gaa gac cca gat aac gtt gcg gcg atg tct tcg ggt cgg Leu Asp Met Giu Asp Pro Asp Asn Val Ala Ala Met Ser Ser Gly Arg 25 ggc gca aaa ctg act cgt ccg cgt cca ggc cac gct gat tac gca ggc Gly Ala Lys Leu Thr Arg Pro Arg Pro Gly His Ala Asp Tyr Ala Gly WO 01/00843 PCTIIBOO/00923 40 atg ctc aag tac gga ttc gat gat gcc cgc aac gtg ctg gag cgt tct 192 Met Leu Lys Tyr Gly Phe Asp Asp Ala Arg Asn Val Leu Giu Arg Ser 55 tca gcc cgt gag acg gca gca cgc gtg gca gca gca acc gtt gcg cgt 240 Ser Ala Arg Glu Thr Ala Ala Arg Val Ala Ala Ala Thr Val Ala Arg 70 75 tcc ttc ctg cgt gaa acc ttg ggc gtg gaa gtg ctt tcc cac gta att 288 Ser Phe Leu Arg Giu Thr Leu Gly Val Glu Val Leu Ser His Val Ile 90 tcc att ggt gcg tcc gag cct tac act ggc gcg gag cca acc ttt gca 336 Ser Ile Gly Ala Ser Glu Pro Tyr Thr Gly Ala Glu Pro Thr Phe Ala 100 105 110 gat att caa gca atc gat gat tcc cca gtt cgt gca ttc ggt aaa gac 384 Asp Ile Gin Ala Ile Asp Asp Ser Pro Val Arg Ala Phe Gly Lys Asp 115 120 125 gct gaa gaa tcc atg atc gcg gaa atc gag gcc gca aag aaa gcc ggc 432 Ala Glu Giu Ser Met Ile Ala Glu Ile Glu Ala Ala Lys Lys Ala Gly 130 135 140 gat acc ctc ggt ggc atc gtg gaa gtg att gtt gaa ggc ctg ccc atc 480 Asp Thr Leu Gly Gly Ile Val Giu Val Ilie Val Glu Gly Leu Pro Ile 145 150 155 160 ggt ttg ggc tca cac att tct ggc gaa gat cgc ctc gat gcg cag atc 528 Gly Leu Gly Ser His Ile Ser Gly Giu Asp Arg Leu Asp Ala Gin Ile 165 170 175 gca gct gca ctc atg ggc att cag gcc atc aag ggc gtg gaa atc ggt 576 Ala Ala Ala Leu Met Gly Ile Gin Ala Ile Lys Gly Val Glu Ile Gly 180 185 190 gac ggt ttc gaa gaa gct cgt cga cgt ggc tcc gaa gcc cac gat gaa 624 Asp Gly Phe Glu Glu Ala Arg Arg Arg Gly Ser Glu Ala His Asp Glu 195 200 205 gtg ttc ctg gat gac aac ggc gta tac cgc aac acc aac cgt gca ggt 672 Val Phe Leu Asp Asp Asn Gly Val Tyr Arg Asn Thr Asn Arg Ala Gly 210 215 220 ggc ctc gaa ggc ggc atg acc aac ggt gaa acc ctg cgc gtt cgt gct 720 Gly Leu Glu Gly Gly Met Thr Asn Gly Glu Thr Leu Arg Val Arg Ala 225 230 235 240 ggc atg aag cca att tct act gtg cct cgc gcc ctg aaa acc att gat 768 Gly Met Lys Pro Ilie Ser Thr Val Pro Arg Ala Leu Lys Thr Ile Asp 245 250 255 atg gaa aac ggc aag gca gca acc gga atc cac cag cgt tcc gac gtg 816 Met Giu Asn Gly Lys Ala Ala Thr Gly Ile His Gin Arg Ser Asp Val 260 265 270 tgc gct gtt cca gcc gcc ggt gtc gtt gca gaa gca atg gtc acc ctg 864 Cys Ala Val Pro Ala Ala Gly Val Val Ala Glu Ala Met Val Thr Leu 275 280 285 WO 01/00843 WO 0100843PCTJIBOO/00923 gtt ctc gcc cgc gca gtc ctg cag aaa ttc ggc ggt gac tcc ctg agc Val Leu Ala Arg Ala Val Leu Gin Lys Phe Gly Gly Asp Ser Leu Ser 290 295 300 gaa acc aag agc aac att gac acc tac ctc aaa aac att gag gaa cga Giu Thr Lys Ser Asn Ile Asp Thr Tyr Leu Lys Asn Ile Giu Giu Arg 305 3.O315 320 atg aaa ttc gaa ggt tta gag gat gga gcg taatgaagtg aatgatcaaa 1010 Met Lys Phe Giu Gly Leu Giu Asp Gly Ala 325 330 ttc 1013 <210> 428 <211> 330 <212> PRT <213> Corynebacteriun giutaxnicum 912 960 <400> 428 Gly Asn Thr Glu Trp, Asp Lys Trp Thr 1 Leu Gly Met Ser Ser Ser Asp Al a Asp 145 Gly Ala Pro Arg Phe Ala 70 Thr Glu Asp Ilie Ile 150 Ile Gly Asn Arg Asp Arg Gly Tyr Ser 120 Giu Giu Gly Gin Val Pro Ala Val Val Thr 105 Pro Ile Val Glu Ala 185 Thr 10 Ala Gly Arg Ala Glu Gly Val Giu Ile Asp 170 Ile Ile Ala His Asn Ala 75 Val Ala Arg Ala Val 155 Arg Lys Met Met Ala Val Ala Leu Glu Ala Ala 140 Glu Leu G ly Ser Ser Tyr Giu Val His Thr 110 Gly Lys Leu Ala Glu 190 His Asp Gly Ala Arg Ala Val Phe Lys Ala Pro Gin 175 Ile Asp Ala Arg Gly Ser Arg Ile Ala Asp Gly Ile 160 Ile Gly Glu Asp Gly Phe Glu Giu Ala Arg Arg Arg Gly Ser Glu Ala WO 01/00843 WO 0100843PCT/EBOO/00923 Val1 Gly 225 Gly Met Cys Val1 Giu 305 Met Phe- 210 Leu Met Giu Ala Leu 290 Thr Lys 195 Leu Giu Lys Asn Val1 275 Ala Lys Phe 200 Val Asn Val Thr Val 280 Gin Thr Asp Tyr Gly Pro Gly 265 Val Lys Tyr Gly Arg Glu Arg 250 Ile Ala Phe Leu Ala 330 Thr 220 Leu Leu Gin Ala Gly 300 Asn Gly Ala 240 Asp Val Leu Ser Arg 320 <210> 429 <211> 906 <212> DNA <213> Corynebacteriun giutamicum <22 0> <221> CDS <222> (101)..(883) <223> RXA01095 <400> 429 gaaaccccag gtcaaagcta gggtgtggca ccctgatttc ataaccttaa acacagcatt ggttggaagg aggttggggc aac Asn acg Thr acg Thr g tc Val1 gat cgc Arg ctt Leu gc g Ala gta Val1 cat atg Met gg t Gly gc t Ala gtc Val gtg.

ttg Leu tt t Phe t tc Phe gcc Al a gac gcg Ala act Thr 30 gtc Val gca Ala gtt 631 ata Ile tct Ser ttt Phe aat Asn ggt tttcgccatg tgtgttcggg atg gtt gca aca gag Met Val Ala Thr Glu 1 tcg gct cgg gaa gca Ser Ala Arg Glu Ala cga tca gca ggt ctc Arg Ser Ala Gly Leu tcg tct gga tgt aat Ser Ser Gly Cys Asn tgg tct gat cat tcg Trp Ser Asp His Ser ttt ggc gca tca atg WO 01/00843 WO 0100843PCT/EBOO/00923 Asp His Val Asp Tyr Ala Gin Val Ala Phe Gly Ala Ser ctt gca tat acg Leu Ala Tyr Thr cgc agg gga cag Arg Arg Gly Gin gat acc gca gta Asp Thr Ala Val cgc gat Arg Asp 100 atc agg gac Ilie Arg Asp ccc atc atc Pro Ilie Ile 120 aaa tct gaa gta Lys Ser Glu Val att ccc att ctg Ile Pro Ile Leu ctt cat gat Leu His Asp 115 atg ggc atc Met Gly Ile gat ccg tat caa Asp Pro Tyr Gin cac gaa gcc cgc His Giu Ala Arg gac gct Asp Ala 135 ctt caa ttc ccc Leu Gin Phe Pro tgg gcg atg gaa Trp Ala Met Glu gct cga ctg gaa Ala Arg Leu Glu ttg gtg gac cgc acc gaa tca ttg ggc Leu Val Asp Arg Thr Giu Ser Leu Gly 155 a tg Met 160 aca gcc atc gtg Thr Ala Ile Val gtg cga aac cac Val Arg Asn His gaa Glu 170 gaa gcg cat cgt Glu Ala His Arg gtg gac gca gga Val Asp Ala Gly gcg aca Ala Thr 180 gtg gta gca Val Val Ala gaa gcg ttt Glu Ala Phe 200 att Ilie 185 gat att act ggt Asp Ile Thr Gly acc ggc tca ctc Thr Gly Ser Leu act ttg cct Thr Leu Pro 195 gta gcc cgc Val Ala Arg tcg ggt atc acc Ser Gly Ilie Thr ttc atg ccc aaa Phe Met Pro Lys gag Glu 210 att gtg Ile Val 215 ctc gga ggt Leu Gly Gly tgc. agc Cys Ser 220 agc cct aaa gaa Ser Pro Lys Glu atg cgg ttt gca Met Arg Phe Ala cga Arg 230 cat tct gca gac gcc atc ttt gtt cca His Ser Ala Asp Ala Ilie Phe Val Pro gca gac ctc gcc Ala Asp Leu Ala aca aaa tct ctt gtg aca gca ggt atg Thr Lys Ser Leu Val Thr Ala Gly Met 250 tgaagaggtg ctctgtggtc agc <210> 430 <211> 261 <212> PRT <213> Corynebacteriun giutamicun <400> 430 Met Val Ala Thr Glu Asn Arg Met Leu 1 5 Ser Ala Arg Giu Ala Thr Leu Gly Phe 25 cca gcg tgc cca Pro Ala Cys Pro tcg cgt Ser Arg 260 Glu Ile Ala Ala Glu Ile Gin Glu Val Lys Thr Lys Ser WO 01/00843 WO 0100843PCTJIBOO/00923 Arg Ser Ala Gly Leu Thr Ala Ala Phe Asp Ile Ala Ser Val Phe Phe 40 Ser Ser Gly Cys Asn Val Val Val Ala Phe Asp Arg Phe Ala Ser Asn 55 Trp Ser Asp His Ser Asp His Val Asp Tyr*Ala Ala Gin Val Ala Gly 70 75 Phe Gly Ala Ser Met Leu Ala Tyr Thr Val Arg Arg Gly Gin Phe Asp 90 Thr Ala Val Arg Asp Ile Arg Asp Ile Lys Ser Giu Val Asp Ile Pro 100 105 110 Ilie Leu Leu His Asp Pro Ile Ile Asp Pro Tyr Gin Ile His Glu Ala 115 120 125 Arg Val Met Gly Ile Asp Ala Leu Gin Phe Pro Val Trp Ala Met Giu 130 135 140 Gin Ala Arg Leu Giu Ser Leu Val Asp Arg Thr Glu Ser Leu Gly Met 145 150 155 160 Thr Ala Ile Val Ser Val Arg Asn His Giu Glu.Ala His Arg Ala Val 165 170 175 Asp Ala Gly Ala Thr Val Val Ala Ile Asp Ile Thr Gly Tyr Thr Gly 180 185 190 Ser Leu Thr Leu Pro Giu Ala Phe Ser Gly Ile Thr Gin Phe Met Pro 195 200 205 Lys Giu Val Ala Arg Ile Val Leu Gly Gly Cys Ser Ser Pro Lys Giu 210 215 220 Leu Met Arg Phe Ala Arg His Ser Ala Asp Ala Ile Phe Val Pro His 225 230 235 240 Ala Asp Leu Ala Thr Thr Lys Ser Leu Val Thr Ala Gly Met His Pro 245 250 255 Ala Cys Pro Ser Arg 260 <210> 431 <211> 1545 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS <222> (101)..(1522) <223> RXA00955 <400> 431 gatggtgcac aaaaggcgct ttccttgctt gccgacggca ccacccaggc atggttggcc aagcacgaag agatcgatta ctcagaaaag gagtcttcca atg act agt aat aat 115 633 WO 01/00843 WO 0100843PCTIIBOOIOO923 Met 1 Thr Ser ASn ctg ccc aca gtg Leu Pro Thr Val ttg Leu gaa agc atc gtC Giu Ser Ile Val ggt cgt cgc gga Gly Arg Arg Gly cac ctg His Leu gag gaa att Giu Glu Ilie gct cgc atc gct Ala Arg Ile Ala gtg gat gtg gat Val Asp Val Asp gcg ctt cca Ala Leu Pro agg gga ggg Arg Gly Gly aaa tcc acc cgt tct ctg ttt gat tcc ctc aac cag Lys Ser Thr Arg Ser Leu Phe Asp Ser Leu Asn Gin gcg cgt Ala Arg ttc atc atg gag Phe Ile Met Giu aag tcc gca tcg Lys Ser Ala Ser cct Pro tct ttg gga atg Ser Leu Gly Met cgt gag cac tac Arg Giu His Tyr ccg ggt gaa atc Pro Gly Giu Ile cgc gtg tac tct Arg Vai Tyr Ser tac gcc agc ggc Tyr Ala Ser Gly at t Ile tcc gtg ctg tgc Ser Val Leu Cys ccg gat cgt ttt Pro Asp Arg Phe ggt ggc Gly Gly 100 gat tac gat Asp Tyr Asp ctg tgc aaa Leu Cys Lys 120 ctc gct acc gtt Leu Ala Thr Val gct acc tct cat ctt ccg gtg Ala Thr Ser His Leu Pro Vai 115 gac ttc atc att Asp Phe Ile Ilie cct gtc cag gta Pro Val Gin Val gcg gcg cgt Aia Ala Arg tac ttt Tyr Phe 135 ggt gct gat gcc atc ctg ctc atg ctc Gly Ala Asp Ala Ile Leu Leu Met Leu tct Ser 145 gtg ctt gat gat Val Leu Asp Asp gag tac gca gca ctc gct gcc gag gct Giu Tyr Ala Ala Leu Ala Aia Giu Ala 155 cgt ttt gat ctg Arg Phe Asp Leu atc ctc acc gag Ile Leu Thr Giu gtt Val1 170 att gat gag gag Ilie Asp Giu Giu gtc gcc cgc gcc Vai Ala Arg Ala atc aag Ile Lys 180 ctg ggt gcg Leu Gly Aia tcc att gat Ser Ilie Asp 200 atc ttt ggc gtc aac cac cgc aac ctg Ile Phe Gly Vai Asn His Arg Asn Leu 190 cat gat ctg His Asp Leu 195 att cca gca Ile Pro Ala ttg gat cgt tca Leu Asp Arg Ser cgc ctg tcc aag Arg Leu Ser Lys C tC Leu 210 gat gcc Asp Ala 215 gtg ctc gtg tct Val Leu Val Ser gag Giu 220 tct ggc gtg cgc Ser Giy Vai Arg acc gaa acc gtc Thr Glu Thr Val cgc cag cta ggt ggg cac Arg Gin Leu Giy Gly His tcc aat gca Ser Asn Ala ttc ctc gtt ggc tcc cag ctg Phe Leu Val Gly Ser Gin Leu WO 01/00843 230 acc agc cag Thr Ser Gin ccc aac aaa Pro Asn Lys cgc gca gcg Arg Ala Ala 280 cca cgc aat 1027 Pro Arg Asn 295 ccc aac ctg 1075 Pro Asn Leu 310 gat ttg ctt 1123 Asp Leu Leu cag gac agc 1171 Gin Asp Ser gag gtt gga 1219 Glu Val Gly 360 ccc ttg ggg 1267 Pro Leu Gly 375 cta att ctt 1315 Leu Ile Leu 390 gct acg gtg 1363 Ala Thr Val atc tct ccg 1411 Ile Ser Pro ttg gac atc 1459 gaa Giu gtc Val 265 ggt Gly gtt Val cgc Arg g tc Val gtc Val 345 ccg Pro gct Ala gat Asp ccg Pro gac Asp 425 aac aac Asn 250 tgc Cys gcg Ala tca Ser tac Tyr gac Asp 330 gaa Glu cag Gin gaa Giu gcc Ala gcc Al a 410 aac Asn tct gcc Ala 255 cca Pro C tc Leu caa Gin cgt Arg gta Val 335 ttg Leu cgc Arg gtg Val agc Ser aag Lys 415 ctc Leu ccc 240 cgc Arg agt Ser atc Ile aaa Lys cgc Arg 320 caa Gin atc Ile gcg Ala gag Glu ggg Gly 400 tc t Ser gc t Ala gcc gaa Glu gca Ala t tc Phe atc Ile 305 acc Thr atc Ile gcc Ala atc Ile ggt Gly 385 gaa Glu ttg Leu gtg Val ggt tta Leu gca Ala gaa Giu 290 atc Ile tcc Ser cac His gcc Ala tcg Ser 370 gac Asp gta Val c tc Leu ggc Gly gca gtc Val caa Gin 275 gag Glu gcc Al a 99g Gly gcc Ala gtt Val1 355 atg Met gtc Val ttc Phe gcg Ala tgc Cys 435 ggC PCT/1B00100923 245 tac ggc 883 Tyr Gly 260 acc gct 931 Thr Ala gca tcg 979 Ala Ser gca gag Ala Giu tac aag Tyr Lys 325 cca ctg Pro Leu 340 cgt gaa Arg Giu tcc agc Ser Ser gat aag Asp Lys gac tgg Asp Trp 405 gga ggc Gly Gly 420 gca ggt Ala Gly acg tgg WO 01/00843 PCTIIBOO/00923 Leu Asp Ile Asn Ser Gly Val Giu Tyr Pro Ala Gly Ala Gly Thr Trp 440 445 450 gct ggg gcg aaa gac gcc ggc gcg ctg ctg aaa att tta gcg acc atc 1507 Ala Gly Ala Lys Asp Ala Gly Ala Leu Leu Lys Ile Leu Ala Thr Ile 455 460 465 tcc aca ttc cat tac taaaggttta aataggatca tga 1545 Ser Thr Phe His Tyr 470 <210> 432 <211> 474 <212> PRT <213> Corynebacterium glutaxnicum <400;, 432 Met Thr Ser Asn Asn Leu Pro Thr Val 1 Arg Val Gin Pro Arg Asp Ser Val Ser 145 Arg Ala Asn His Leu Giy Gly Ser Gly 100 Pro Ala Asp Leu Ile 180 Asp 5 Leu Pro Gly Met Arg Gly Val Arg Asp Asp 165 Lys Leu Glu Lys Al a Ile Tyr Asp Leu Tyr Glu 150 Ile Leu Ser Leu 10 Ala Ser Met Tyr Ilie 90 Leu Phe Asp Ala Val1 170 Ile Asp Val Ile Ala Asp Lys Gly Leu Val Asp 125 Leu Ala Glu Val1 Arg 205 Ser Val His Ser Ser Glu Cys Ala 110 Pro Leu Glu Glu Asn 190 Arg Gly Giu Val Leu Al a Ile Glu Ala Val Met Ala Glu 175 His Leu Val Lys Leu 210 Ile Pro Ala Asp Ala Val Leu 215 WO 01/00843 WO 0100843PCTIBOO/00923 Asp Thr Glu Thr Val Arg Gin Leu Gly Gly His Ser Asn Ala Phe Leu 225 230 235 240 Val Gly Ser Gin Leu Thr Ser Gin Giu Asn Val Asp Leu Ala Ala Arg 245 250 255 Glu Leu Val Tyr Gly Pro Asn Lys Val Cys Gly Leu Thr Ser Pro Ser 260 265 270 Ala Ala Gin Thr Ala Arg Ala Ala Gly Ala Val Tyr Gly Gly Leu Ile 275 280 285 Phe Giu Glu Ala Ser Pro Arg Asn Val Ser Arg Giu Thr Leu Gin Lys 290 295 300 Ilie Ile Ala Ala Glu Pro Asn Leu Arg Tyr Val Ala Val Ser Arg Arg 305 310 315 320 Thr Ser Gly Tyr Lys Asp Leu Leu Val Asp Gly Ile Phe Ala Val Gin 325 330 335 Ilie His Ala Pro Leu Gin Asp Ser Val Giu Ala Glu Lys Ala Leu Ile 340 345 350 Ala Ala Val Arg Giu Giu Val Gly Pro Gin Val Gin Val Trp Arg Ala 355 360 365 Ile Ser Met Ser Ser Pro Leu Gly Ala Giu Val Ala Ala Ala Val Glu 370 375 380 Gly Asp Val Asp Lys Leu Ile Leu Asp Ala His Glu Gly Gly Ser Gly 385 390 395 400 Glu Val Phe Asp Trp Ala Thr Val Pro Ala Ala Val Lys Ala Lys Ser 405 410 415 Leu Leu Ala Gly Gly Ilie Ser Pro Asp Asn Ala Ala Gin Ala Leu Ala 420 425 430 Val Gly Cys Ala Gly Leu Asp Ile Asn Ser Gly Val Giu Tyr Pro Ala 435 440 445 Gly Ala Gly Thr Trp Ala Gly Ala Lys Asp Ala Gly Ala Leu Leu Lys 450 455 460 Ilie Leu Ala Thr Ilie Ser Thr Phe His Tyr 465 470 <210> 433 <211> 494 <212> DNA <213> Corynebacterium giutanicun <220> <221> CDS <222> <223> RXA02814 <400> 433 WO 01/00843 gcc Ala 1 atc Ile ctg Leu ac c Thr cac His c tc Leu ggc Gly tqc Cys gga Gly acc Thr aaa Lys c tg Leu acc Thr C tC Leu ccc Pro atc Ile tgg Trp gcc Ala ggc Gly 130 gcg Ala cta gaa gaa cac tcc tac gtg gtc aac cac ctg Leu Glu Glu His Ser Tyr Val Val Asn His Leu 5 10 cca Pro ac c Thr tac Tyr ccc Pro gaa Glu gac Asp 100 gta Val1 gtc Val1 tgc Cys gaa Glu cac His atc Ile 70 gaa Glu acc Thr gaa Giu gaa Glu tca Ser atg Met atc Ile 55 tgt Cys tcc Ser gga Gly gac Asp tca Ser 135 ttc gac Phe Asp 25 cac ctc His Leu gca cta Ala Leu acc ccc Thr Pro cga aac Arg Asn ggc gaa Gly Glu 105 acc tgg Thr Trp gcc caa Ala Gin cca Pro aca Thr c ta Leu gac Asp t ac Tyr atg Met aga Arg gag Glu 140 aca Thr ccc Pro gcc Ala gcc Ala gcc Ala gta Val1 gca Ala 125 ttt Phe gtt Val1 atc Ile ata Ile gcc Ala gga Gly gcc Ala 110 tgg Trp ga t Asp PCTIBOOOO923 cgc acc 48 Arg Thr cct gaa 96 Pro Glu gtt ggc 144 Val Gly cga aca 192 Arg Thr gaa gcc 240 Glu Ala gca gcc 288 Ala Ala atc cgc 336 Ile Arg gca ggc 384 Ala Gly gaa acc 432 Glu Thr aag ctc caa acc atc atg cgc tcg ctt Lys Leu Gin Thr Ile Met Arg Ser Leu ggt ttg tgagatgtgg Gly Leu tcttaaaaca ccg <210> 434 <211> 157 <212> PRT <213> Corynebacteriun giutamicum' <400> 434 Ala Lys Asn Leu Glu Glu His Ser Tyr 1 5 Ile Leu Glu Pro Leu Cys Ser Gin Phe 25 Leu Thr Lys Thr Asn Glu Met Trp His 40 Thr Leu Lys Tyr Pro His Ile Thr Ala 55 Val Val Asn His Leu Arg Thr 10 Asp Ala Pro Thr Val Pro Glu Leu Ala Thr Pro Ile Val Gly Leu Glu Leu Ala Ile Arg Thr WO 01/00843 His Pro Thr Pro Ala Ile Cys Gly 1 70 Leu Ile Ile Giu Ala Giu Ser Pro 1 Giy Trp Cys Asp Ser Thr Giy AspC 100 Cys Ala Giu Val Ser Giu Asp Gly 115 120 Giy Gly Ilie Val Ala Glu Ser Asp 130 135 Thr Ala Lys Leu Gin Thr Ilie Met 145 <210> 435 <211> 803 <212> DNA <213> Corynebacterium giutanicun PCT1B00100923 Ala Giu Ala Giy Ala Ala Ala Ile Arg 110 Trp Ala Gly Asp Giu Thr <220> <221> CDS <222> <223> RXA00229 <400> 435 gag gcg Giu Ala 1 gac acg Asp Thr gac gcc Asp Ala tac aag Tyr Lys acc caa Thr Gin acc acc Thr Thr gaa ggc Giu Gly gcg cag gga cgt gcg Ala Gin Gly Arg Ala acg gtg tac agg cgc atc Thr Val Tyr Arg Arg Ile ggg Gly agc Ser ctt Leu g tc Val1 tcc Ser gat Asp 105 caa Gin Gly ggc Gly gt t Val1 gga Gly tcc Ser gat Asp ctg Leu gaa Giu atg Met ttt Phe gtc Val ctc ggc Leu Gly ggc cac Gly His ctc ccc Leu Pro 100 ggc ggc gta gaa aac gca gtg gca tac Gly Gly Val Glu Asn Ala Val Ala Tyr 115 120 gcc ctg gtc Ala Leu Val WO 01/00843 WO 0100843PCTAlBOO/00923 cag aaa ctt cag gtc gct gac ctc gac act tcc cgc gcg cag gca ctg .432 Gin Lys Leu Gin Val Ala Asp Leu Asp Thr Ser Arg Ala Gin Ala Leu 130 135 140 gca gat gtc atc aac aac gca gtc ggc cgt gaa gcc gtc gtg gga gta 480 Ala Asp Val Ile Asn Asn Ala Val Gly Arg Giu Ala Val Val Gly Val 145 150 155 160 gac gcc cgc ggc atc gaa gac gtc atc gcc gcc gcc gac gga gta gtc 528 Asp Ala Arg Gly Ilie Giu Asp Val Ile Ala Ala Ala Asp Gly Val Val 165 170 175 aac gca acc ccc atg gga atg cca gca cac ccc ggc acc gcc ttt gat 576 Asn Ala Thr Pro Met Gly Met Pro Ala His Pro Gly Thr Ala Phe Asp 180 185 190 gtc agc tgc ctc acc aag gat cac tgg gtt ggc gac gtc gtg tac atg 624 Val Ser Cys Leu Thr Lys Asp His Trp Val Gly Asp Val Val Tyr Met 195 200 205 ccc atc gaa act gaa ctt ctc aaa gcc gcc cgt gcc ctc ggc tgc gaa 672 Pro Ile Giu Thr Giu Leu Leu Lys Ala Ala Arg Ala Leu Gly Cys Giu 210 215 220 acc ctc gac gga acc cgc atg gca atc cac caa gcc gtc gat gcc ttc 720 Thr Leu Asp Gly Thr Arg Met Ala Ile His Gin Ala Val Asp Ala Phe 225 230 235 240 cga ctg ttc acc ggc ctc gaa ccc gac gtc tcc cgc atg cgg gaa act 768 Arg Leu Phe Thr Gly Leu Giu Pro Asp Val Ser Arg Met Arg Glu Thr 245 250 255 ttc ctg tcc ctc taaaagagtc agtaaaacct cga 803 Phe Leu Ser Leu 260 <210> 436 <211> 260 <212> PRT <213> Corynebacteriui glutainicum <400> 436 Giu Ala Lys Gly Leu Ala Gin Gly Arg Ala Thr Val Tyr Arg Arg Ile 1 5 10 Asp Thr Leu Gly Ser Arg Ala Ser Gly Gin Asp Leu Asn Thr Leu Leu 25 Asp Ala Ala Leu Tyr Leu Gly Phe Ser Gly Leu Asn Ile Thr His Pro 40 Tyr Lys Gin Ala Val Leu Pro Leu Leu Gly Giu Val Ser Glu Gin Ala 55 Thr Gin Leu Gly Ala Val Asn Thr Val Val Met Asp Ala Thr Gly His 70 75 Thr Thr Gly His Asn Thr Asp Val Ser Gly Phe Gly Arg Gly Met Giu 90 WO 01/00843 WO 0100843PCTJLBOOIOO923 Giu Gly Gin Ala 145 Asp Asn Val Pro Thr 225 Arg Phe Pro 100 Glu Gin Ile Gly Pro 180 Leu Thr Gly Thr Leu 260 Asn Asn Val1 Asn Ile 165 Met Thr Glu Thr Gly 245 Ala Aia Aia Asn 150 Giu Gly Lys Leu Arg 230 Leu Leu Ala 120 Leu Val Vai Pro His 200 Lys Ala Pro Asp 105 Tyr Asp Gly Ile Ala 185 Trp Ala Ile Asp Ser Ala Thr Arg Ala 170 His Val1 Ala His Val 250 Val Leu Ser Gi u 155 Ala Pro Gly Arg Gin 235 Ser Val 110 His Gin Val Gly Ala 190 Val Gly Asp Arg Gly Gly Ala G ly Val 175 Phe Tyr Cys Ala Giu 255 <210> 437 <211> 927 <212> DNA <213> Corynebacterium glutainicum <2 <221> CDS <222> (101)..(904) <223> RXA02093 <400> 437 ggcaggaatt tcccgaaaac ttccaccaat aatcaagcca ttgctcttcc tttgctccgc atgagtataa aatcactgtc gac agg gaa aca acc ctg tgc atc tct ctc gct Asp Arg Giu Thr Thr Leu Cys Ile Ser Leu Ala 15 cat ggt gtt cgt ttc cac aac tgg ctt tac gct His Gly Val Arg Phe His Asn Trp Leu Tyr Ala 30 tac ctg tac aag gct gtt gcc cca gca gat atc 641 tatcccacac aatcaggcat atg gtc aac tac gtc 115 Met Val Asn Tyr Val 1 gct cgt cca tcc aac 163 Ala Arg Pro Ser Asn gaa ctt gga ttg aac 211 Glu Leu Gly Leu Asn acc gct gca gtc gca 259 WO 01/00843 Tyr Leu Tyr Lys Ala Vai Ala Pro Ala Asp Ile Thr 45 ggt Gly aag Lys cgc Arg gga Gly cgc Arg gcc Ala gtc Val 150 gg t Gly aa t Asn ttt Phe gca Ala aag Lys 230 cag Gin atc Ile agc Ser ata Ile tac Tyr gtg Val aat Asn 135 gtt Vai tgg Trp gta Vai ggt Gly ttc Phe 215 c aa Gin t tc Phe cgt Arg gat Asp cgt Arg aac Asn aac Asn 120 gc t Al a gcc Ala gaa Glu acc Thr gag Glu 200 ccc Pro ac c Thr cac His ggt Giy gtc Val tct Ser acc Thr 105 ccc Pro gtt Val cgc Arg t ac Tyr cca Pro 185 gat Asp gtc Val atc Ile ctc Leu gag G iu 265 ctg Leu atc Ile gtt Val1 gac Asp aat Asn gt t Val1 aac Asn tcc Ser 170 atg Met gaa Giu gag Giu gac Asp tac Tyr 250 aac Asn cca Pro 75 aac Asn tac Tyr gca Ala gc a Aia cac His 155 gca Ala gga Gly gta Val acc Thr ggc Gly 235 acc Thr att Ile 60 c tc Leu acc Thr act Thr cga Arg gct Ala 140 acc Thr act Thr atg Met gac Asp cca Pro 220 gga Gly gga Gly ggt Gly t tg Leu 80 aat Asn c ac His aag Lys tat Tyr gcg Ala 160 gac Asp gac Asp gta Val1 ttg Leu gc t Ala 240 acc g tc Val cat His gac Asp ctc Leu gga Gly ggt Gly 145 cta Leu gc a Ala c aa Gin atc Ile gcc Ala 225 ctt Leu aac PCTLBOO/00923 Ala Ala Val Ala tcc atg cca tac 307 Ser Met Pro'Tyr cct tcc gca gag 355 Pro Ser Ala Glu gga cac ctt gtc 403 Gly His Leu Val 100 ctt gaa gaa cac 451 Leu Giu Giu His 115 tcc ggc ggc atg 499 Ser Gly Gly Met 130 ctg agt ggc acc 547 Leu Ser Gly Thr gct tcc cgt tac 595 Ala Ser Arg Tyr 165 aaa att ttg gtt 643 Lys Ile Leu Val 180 gac gtt gta tct 691 Asp Val Val Ser 195 ttt gac tgc gta 739 Phe Asp Cys Val 210 aag gaa aag ggt 787 Lys Glu Lys Gly cag gca gca gag 835 Gin Ala Ala Giu 245 gac cag atc att 883 Asp Gin Ile Ile 260 Val Leu Pro Thr Asn Ala Ala Giu <210> 438 ttc tcc aag taaatttctc tcccctattt tta Phe Ser Lys WO 01/00843 <211> 268 <212> PRT <213> Corynebacteriul glutamicum <400> 438 Met Val Asn Tyr Val Asp Arg Glu Thr PCTIIBOO/00923 1 Ala Giu Thr Val His Asp Leu Gly Gly 145 Leu Ala Gin Ile Ala 225 Leu Asn Arg Leu Ala Ser Pro Gly Leu Ser 130 Leu Ala Lys Asp Phe 210 Lys Gin Asp Pro Gly Ala Met Ser His Giu 115 Gly Ser Ser Ile Val 195 Asp Giu Ala Gin Ser Leu Val Pro Ala Leu 100 Giu Gly Gly Arg Leu 180 Val Cys Lys Ala Ile 260 5 Asn Asn Ala Tyr Glu Val His Met Thr Tyr 165 Val Ser Val1 Gly Glu 245 Ile His Tyr Gly Lys Arg Gly Arg Ala Val1 150 G ly Asn Phe Ala Lys 230 Gin Al a Gly Leu Ile Ser Ile Tyr Val Asn 135 Val Trp Val Gly Phe 215 Gin Phe Ala Thr 10 Phe Ala Leu Ile Val 90 Asp Asn Val Asn Ser 170 Met Glu Giu Asp Tyr 250 Phe Ser Leu Ala Gly Asp Val Val 110 Ala Al a Gly Pro Gly 190 Al a Ile Val1 Leu Leu Tyr Asp Ala Giu Asn Tyr Ile Glu Ser Glu 175 Pro Asp Lys Ala Pro 255 <210> 439 <211> 951 <212> DNA <213> Corynebacteriun glutamicum WO 01/00843 WO 0100843PCTIiBOO/00923 <220> <221> CDS <222> (101) (928) <223> RXA02791 <400> 439 accgtgttca acgacacctt cgaagagcac gaagccgaca ttgagcaagc tttgaacagt ggcgttctag acagcaaccg ataaggatca gcgaataaaa ttg ggt tct cac Leu Gly Ser His* act cac cgg gcg gcc gta ctc ggc tca ccc atc gag cat tcc Thr His Arg Ala Ala Val Leu Gly Ser Pro Ile Giu His Ser aaa tcc Lys Ser cca gtc ctc Pro Val Leu gaa tac gac Giu Tyr Asp cac His aac acc ggc tat Asn Thr Gly Tyr gcc ctc gga ctg Ala Leu Gly Leu gac caa tgg Asp Gin Trp ggc atc gtc Gly Ile Val cgc ttt gag tgc Arg Phe Glu Cys ggc gac atg ctc Gly Asp Met Leu tcc ggc Ser Gly gct gat gaa acc Ala Asp Giu Thr tgc gga ttc tcc Cys Gly Phe Ser acc atg ccg tct Thr Met Pro Ser aaa Lys ttc gca gct ctt Phe Ala Ala Leu ttc gcc gac gaa Phe Ala Asp Glu acc gaa cgc gcc Thr Giu Arg Ala gcc atc ggc tcc Ala Ilie Gly Ser aac acc ttg ctg Asn Thr Leu Leu acg gcc acc gga Thr Ala Thr Gly tgg cgc Trp, Arg 100 gcc gac aac Ala Asp Asn ctc ggc ggc Leu Gly Gly 120 gac gtc gac ggc Asp Val Asp Gly atc Ile 110 agg gga gct ctc Arg Gly Ala Leu ggt gaa ctc Gly Giu Leu 115 atc ggc tcc Ile Gly Ser gca tca ctg gcc Ala Ser Leu Ala 99c Gly 125 aaa cac gcc atc LYS His Ala Ile ggc ggc Gly Gly 135 acc gca cgc ccc Thr Ala Arg Pro gcc Ala 140 atc tgg gca ctc Ile Trp Ala Leu atc Ile 145 gaa gcc ggg gtc Giu Ala Gly Val cgg atc acg gtg' Arg Ile Thr Val c tc Leu 155 aac cgc tcc gat Asn Arg Ser Asp acc gcc gaa ctg Thr Ala Glu Leu acg ctt ttc gac Thr Leu Phe Asp gaa Giu 170 acc ccc acc acc Thr Pro Thr Thr gcc tac gcc ccg Ala Tyr Ala Pro ctc gag Leu Giu 180 cat ctc cac His Leu His gaa gcc gac gtc Glu Ala Asp Val gtc tct aca gtg Val Ser Thr Val ccc tcc gca Pro Ser Ala 195 WO 01/00843 gca atc gca ggc ctc gaa gac acc ctt.

Ala Ile Ala Gly Leu Giu Asp Thr Leu 200 205 gtc atc tac gac ccc tgg cca aca cca Val Ilie Tyr Asp Pro Trp Pro Thr Pro 215 220 aaa ggc ctc aaa gct gtc gga ggc cac Lys Gly Leu Lys Ala Val Gly Gly His 230 235 tac gga cag ttt gaa caa ttc acc gga Tyr Gly Gin Phe Giu Gin Phe Thr Gly 250 atg cgt gag gct ttg gaa gag tct tta Met Arg Glu Ala Leu Giu Glu Ser Leu 265 270 taagtccccg ccacctcctc aac <210> 440 <211> 276 <212> PRT <213> Corynebacteriun giutanicuin <400> 440 Leu Gly Ser His Ilie Thr His Arg Ala gcg Ala c tc Leu gtc Vai atg Met 255 ggc Giy atc Ile g ta Val1 atg Met 240 ga t Asp gcc cca Ala Pro 210 gaa gtc Glu Val 225 ctg gca Leu Aia gca ccc Ala Pro PCTIIBOO/00923 gtc ctc gac 739 Val Leu Asp aca cga gcc 787 Thr Arg Ala cac cag tcc 835 His Gin Ser 245 cgc gat gcc 883 Arg Asp Ala 260 gaa cac 928 Glu His atc tca gaa Ile Ser Giu 1 Glu Gly Leu Val1 Thr Ala Ala Ile Ilie 145 Thr 5 Ser Trp Val Ser Cys Arg Leu Ser Val1 Gin 165 Val Tyr Gly Phe Ile Asp Gly Giy 135 Arg Leu Leu Asp Ala Al a Gly Asn Gly 120 Thr Ile Phe Ala Asn Phe Glu Leu Al a Asp Ser Arg Val Glu 170 Val Thr Glu Thr Glu 75 Asn Val1 Leu Pro Leu 155 Thr Leu Gly Cys Tyr Phe Thr Asp Ala Ala 140 Asn Pro Giy Tyr Thr Cys Ala Leu Gly Gly 125 Ile Arg Thr Ile Leu Met Ser Val1 Thr Gly Al a Leu Arg 160 Ala WO 01/00843 WO 0100843PCTIiBOO/00923 Tyr Thr Ala Giu 225 Leu Ala Ser Pro Pro 195 Val Thr His Arg Glu 275 His Ala Val1 Lys 230 Tyr Met Leu Ile Ile 215 Gly Gly Arg His Ala 200 Tyr Leu Gin Giu Ile 185 Gly Asp Lys Phe Ala 265 Ala Giu Trp, Val1 235 Gin Giu Val1 Ala Leu Val Met 255 Gly <210> 441 <211> 693 <212> DNA <213> Corynebacterium glutamicun <22 0> <221> CDS <222> (101)..(670) <223> RXA01699 <400> 441 ctgcagaaat tcggcggtga ctccctgagc gaaaccaaga aaaaacattg aggaacgaat gaaattcgaa ggtttagagg gtg Val tgc Cys tcc Ser gac Asp gtg Val cac aat Asn tcc Ser acc Thr tcc Ser ttc Phe gtg gat caa att Asp Gin Ile tgc ccg atc Cys Pro Ile att gga cgt Ilie Gly Arg gac gaa ctg Asp Glu Leu agc gag ctc Ser Giu Leu gcc gaa gca tta Leu gtt Vai t ta Leu gag Giu 60 gag Glu aaa gat Asp ctt Leu gcg Ala 45 cgc Arg cca Pro tCC gcaacattga cacctacctc atg gag cgt aat gaa Met Glu Arg Asn Glu 1 gat gac acc tct gaa Asp Asp Thr Ser Glu cca gga gct gga aaa Pro Gly Ala Gly Lys aac act gaa ctc gtc Asn Thr Giu Leu Val aaa gcc tgt ggc gcc Lys Ala Cys Gly Ala gag ctc gag gcc atc Giu Leu Giu Ala Ile gtg agc ttg gga ggc WO 01/00843 His Val Ala Giu Ala Leu Lys Ser Ser gga tct gtg ctg aca gaa tcc acc cgt Gly Ser Val Leu Thr Giu Ser Thr Arg 105 110 gtg gtc tgg atc gac gtg cca gta gaa Vai Val Ti-p Ilie Asp Vai Pro Val Giu 120 125 aac gag cgt tcc cgc ccc gtg ctg caa Asn Giu Arg Ser Arg Pro Val Leu Gin 135 140 tac cgc aac ctg gtg aaa gtg cgc acc Tyr Arg Asn Leu Val Lys Val Arg Thr 150 155 acc tac cga ctt cgc acc aac aac cgc Thr Tyr Arg Leu Arg Thr Asn Asn Arg 170 gca gtg ttg cat cat cta gaa atc gat Ala Val Leu His His Leu Giu Ile Asp 185 190 tta <210> 442 <211> 190 <212> PRT <213> Corynebacterium glutamicun <400> 442 Met Giu Arg Asn Giu Vai Asn Asp Gin 1 Asp Asp Thr Ser Giu Cys Ser Cys Pro Pro Gly Ala Giy Lys Ser Thr Ilie Gly 40 Asn Thr Giu Leu Val Asp Ser Asp Glu Lys Ala Cys Gly Ala Val Phe Ser Giu Glu Leu Giu Ala Ile His Val Ala Glu Val Ser Leu Gly Gly Gly Ser Val Leu 100 105 Leu Lys Gly Gin Asp Val Val Ti-p Ilie 115 120 Ile Arg Arg Thr Ala Asn Giu Arg Ser PCTIIBOO/00923 Gly Vai Val Ser Leu Gly Gly 95 100 gaa ctg ctc aaa ggc cag gac 451 Giu Leu Leu Lys Gly Gin Asp 115 gaa ggc atc agg cgc acc gca 499 Glu Gly Ile Arg Arg Thr Ala 130 gcc gcc gac ccc gcc gag cac 547 Ala Ala Asp Pro Ala Giu His 145 ccg ttg tac gaa gag gtg gca 595 Pro Leu Tyr Glu Giu Val Ala 160 165 agc ccc cag caa gtg gtg gca 643 Ser Pro Gin Gin Val Val Ala 175 180 taattaaacc gggcacctga 690 Asp Leu Ala Arg Pro Ser Thi- Val 125 Leu WO 01/00843 WO 0100843PCTIiBOO/00923 135 Asp Pro Ala Giu His Tyr Arg Asn Leu Val Lys 145 150 155 Tyr Glu Glu Val Ala Thr Tyr Arg Leu Arg Thr 165 170 Gin Gin Val Val Ala Ala Val Leu His His Leu 180 185 <210> 443 <211> 959 <212> DNA <213> Corynebacterium giutamicumr Val Arg Thr Pro Asn Asn Arg Ser Pro 175 Glu Ile Asp 190 <220> <221> <222> <223>

CDS

(97)..(936) RXA0 09 52 <400> 443 catcctcgtc tccctatccg gccgtggcga caaggacgtt gaccacgtgc gccgcaccct cgaagaaaat ccagaactga tcctgaagga caaccgatg agc cgt tac gad gat Met Ser Arg Tyr Asp Asp ctt ttt gca Leu Phe Ala atc atg ctg Ile Met Leu cgc Arg ctc gac acg gca Leu Asp Thr Ala gag ggc gcc ttt Glu Gly Ala Phe gtt ccc ttc Val Pro Phe atc atc tcc Ile Ile Ser agc gac cct tca Ser Asp Pro Ser gag gag gct ttc Giu Glu Ala Phe cag Gin aca gca Thr Ala atc gaa gct ggc Ile Glu Aia Gly gat gca ctg gaa Asp Ala Leu Glu ggc gta cct ttc Gly Val Pro Phe tcc Ser gac cca gtt gcc Asp. Pro Val Ala ggc ccc acc gtc Gly Pro Thr Val gaa tcc cac ctc Glu Ser His Leu gca ctc gac ggc Ala Leu Asp Gly gcc acc gta gac Ala Thr Val Asp gca ctc gag cag Ala Leu Glu Gin atc aag Ile Lys cgc gtg cgc Arg Val Arg ggc aac gtt Giy Asn Val 105 gcc tac cca gag Ala Tyr Pro Glu ccc atc gga atg Pro Ile Gly Met ctc atc tac Leu Ile Tyr 100 caa gag ttc Gin Glu Phe cct ttc acc cgt Pro Phe Thr Arg ttg gat cgc ttc Leu Asp Arg Phe tac Tyr 115 gct gaa Ala Giu 120 gct ggc gca gac Ala Gly Ala Asp atc ctc ctg cca Ilie Leu Leu Pro gtc cca gtc cga Val Pro Val Arg WO 01/00843 gaa ggc gca Giu Gly Ala 135 att tac atc Ilie Tyr Ilie tcc gcc gca Ser Ala Ala acc ggc acc Thr Gly Thr 185 gac aac atc Asp Asn Ile 200 atc tca tcc Ilie Ser Ser 215 ggt gcg atc Gly Ala Ilie gaa ggt gag Glu Gly Giu aag aag gat Lys Lys Asp 265 PCTIIBOO/00923 ccg ttt tct Pro Phe Ser 140 gct ccg gcc Ala Pro Ala 155 tca aag ggc Ser Lys Gly 170 gaa cgt gaa Glu Arg Glu aag aaa ttt Lys Lys Phe cct cag cac Pro Gin His 220 acg ggt tcc Thr Giy Ser 235 cac ccg aac His Pro Asn 250 ctc act gag gca gca gct Ala Ala Ala gca gca gcc gga att Ala Ala Ala Giy Ile 145 gag Giu 160 gcc Al a 94C Asp ccc Pro gcg Ala aag Lys 240 att Ile gcg Ala aaa Lys atc Ile Gly atc Ile att Ile 225 atc Ile cga Arg atg Met gat Asp ggt Gly 165 ggc Gly gtg Val ttc Phe gct Ala cac His 245 ggt Gly acc Thr ccc Pro 150 gtc Val gtc Vai gtg Val1 99c Gly tcc Ser 230 tgc Cys ttg Leu aag Lys 546 594 642 690 738 786 834 882 930 959 Leu Thr Glu Phe Ilie Ser aag gtt taggccttta aatgtggcaa tgt Lys Val 280 <210> 444 <211> 280 <212> PRT <213> Cory-nebacteriui giutaricun <400> 444 Met Ser Arg Tyr Asp Asp Leu Phe Ala 1 5 Gly Aia Phe Val Pro Phe Ilie Met Leu 25 Ala Phe Gin Ile Ile Ser Thr Ala Ile 40 Giu Leu Gly Val Pro Phe Ser Asp Pro 55 Ala Glu Ser His Leu Arg Ala Leu Asp 70 Arg Leu Asp Thr Ala Gly Glu 10 Ser Asp Pro Ser Pro Glu Glu Giu Ala Gly Ala Asp Ala Leu Val Ala Asp Gly Pro Thr Val Gly Gly Ala Thr Val Asp Ser 75 WO 01/00843 WO 0100843PCTIBOO/00923 Ala Leu Glu Gin Ile Lys Arg Val Arg Ala Ala Tyr Pro Glu Val Pro 90 Ile Arg Pro Ala 145 Lys Ile Gly Ile Ile 225 Ile Arg Met Gly Phe Asp 130 Ala Thr Ser Leu Leu 210 Al a Ile Asp Lys Met Tyr 115 Vai Gly Leu Arg Ser 195 Leu Ala Ala Met Ala 275 Leu 100 Gin Pro Ile Glu Asp 180 Ala Gly Gly Ser Asp 260 Ala Ile Glu Val Asp Gly 165 Gly Val1 Phe Ala His 245 Giy Thr Gly Ala Giu 135 Ile Ser Thr Asp Ile 215 Gly Giu Lys Lys Asn Glu 120 Gly Tyr Ala Gly Asn 200 Ser Ala Gly Lys Val1 280 Val 105 Ala Al.a Ile Ala Thr 185 Ile Ser Ile Glu Asp 265 Phe Ala Phe Pro 155

LYS

Arg Lys Gin Gly 235 Pro Thr Arg Gly 110 Ser Ile 125 Ala Ala Asn Ala Tyr Ile Ser Ser 190 Asp Gly 205 Val Ala Ala le Pro Ser Phe Ilie 270 Leu Leu Ala Ser Tyr 175 Thr Ala Asp Thr Thr 255 Ser Asp Leu Ala Glu 160 Ala Asp Pro Ala Lys 240 Ile Ala <210> 445 <211> 1237 <212> DNA <213> Corynebacteriun glutanicun <22 0> <221> CDS <222> (101)..(1237) <223> RXN00956 <400> 445 gccggtgcag gcacgtgggc tggggcgaaa gacgccggcg cgctgctgaa aattttagcg accatctcca cattccatta ctaaaggttt aaataggatc atg act gaa aaa gaa 115 Met Thr Glu Lys Giu 1 aac ttg ggc ggc tcc acg ctg ctg cct gca tac ttc ggt gaa ttc ggc 163 Asn Leu Gly Gly Ser Thr Leu Leu Pro Ala Tyr Phe Gly Glu Phe Gly 15 WO 01/00843 ggc cag ttc Giy Gin Phe PCT/iBOOIOO923 gcg gaa tcc ctc Ala Glu Ser Leu ctg Leu cct gct ctc gac Pro Ala Leu Asp cag ctg gag Gin Leu Giu gaa gaa ctc Giu Giu Leu aag gcc ttc gtt gac gcg acc Lys Ala Phe Val Asp Ala Thr agc cca gag ttc Ser Pro Glu Phe ggc ggC Gly Gly tac ctc cgc gat Tyr Leu Arg Asp tac Tyr ctc ggc cgc cca Leu Gly Arg Pro ccg ctg acc gaa Pro Leu Thr Giu tcc aac ctg cca Ser Asn Leu Pro gca ggc gaa ggc Ala Gly Glu Gly ggc ttt gcg cgg Gly Phe Ala Arg ttc ctc aag cgc Phe Leu Lys Arg gac ctc gtc cac Asp Leu Vai His ggt gca cac aaa Gly Ala His Lys act aac Thr Asn 100 cag gtg atc Gin Val Ilie atc atc gca Ile Ilie Ala 120 cag gtg ctg ctt Gin Val Leu Leu aag cgc atg ggc Lys Arg Met Gly aaa acc cgc Lys Thr Arg 115 acc gct ctc Thr Ala Leu 259 307 355 403 451 499 547 595 643 gag acc ggc gca Giu Thr Gly Ala ggc Gly 125 cag cac ggc acc Gin His Gly Thr gca tgt Ala Cys 135 gcg ctc atg ggc Ala Leu Met Gly gag tgc gtt gtc Giu Cys Val Val atg ggc gcc aag Met Gly Ala Lys gac Asp 150 gtt gcc cgc cag Val Ala Arg Gin ccc aac gtc tac Pro Asn Val Tyr cgc Arg 160 atg cag ctg cac Met Gin Leu His ggc Giy 165 gcg aag gtc atc Ala Lys Val Ile gtg gaa tct ggt Val Giu Ser Gly tcc Ser 175 ggc acc ctg aag Gly Thr Leu Lys gac gcc Asp Ala 180 gtg aat gaa Val Asn Glu tac ctt ctc Tyr Leu Leu 200 ctg cgc gat tgg Leu Arg Asp Trp gca acc ttc cac Ala Thr Phe His gag tcc cac Glu Ser His 195 acc atc gtg Thr Ile Val ggc acc ccc gcc Gly Thr Pro Ala ccg cac cca ttc Pro His Pro Phe cgt gaa Arg Glu 215 ttc cac aag gtg Phe His Lys Val tct gag gaa gcc Ser Giu Giu Ala aag Lys 225 gca cag atg cta Ala Gin Met Leu cgc acc ggc aag Arg Thr Gly Lys ccc gac gtt gtg Pro Asp Val Val gcc tgt gtc ggt Ala Cys Val Gly ggc tcc aac gcc Gly Ser Asn Ala ggc atg ttc gca Gly Met Phe Ala ttc att gac gat Phe Ile Asp Asp gaa ggt Glu Gly 260 WO 01/00843 gta gag ctc gtc ggc gct gag cca Val Glu Leu Val Gly Ala Giu Pro 265 aag cac ggc gca acc atc acc aac Lys His Gly Ala Thr Ile Thr Asn 280 285 acc cgt tcc tac ctg atg cgc aac 1027 Thr Arg Ser Tyr Leu Met Arg Asn 295 300 tac tcc atc tcc gcc gga ctt gat 1075 Tyr Ser Ilie Ser Ala Gly Leu Asp 310 315 gca cac ctg cac gcc acc ggc cgc 1123 Ala His Leu His Ala Thr Gly Arg 330 gcc gaa gcc ctc caa gca ttc cag 1171 Ala Giu Ala Leu Gin Ala Phe Gin 345 atc ccc gca ctg gaa tcc tca cac 1219 Ilie Pro Ala Leu Giu Ser Ser His 360 365 gcc aag acc gcc gaa gta 1237 Ala Lys Thr Ala Giu Val 375 <210> 446 <211> 379 <212> PRT <213> Corynebacteriun giutamicun <400> 446 Met Thr Giu Lys Giu Asn Leu Gly C 1 5 Phe Gly Giu Phe Gly Gly Gin Phe N Leu Asp Gin Leu Giu Lys Ala Phe N Phe Arg Giu Giu Leu Gly Giy Tyr I Thr Pro Leu Thr Giu Cys Ser Asn I 70 Gly Phe Ala Arg Ile Phe Leu Lys jcc 270 49 t 3iy :cc 3er :ac ['yr ;cc U.a tac ['yr 350 Icg gaa Giu atc Ile Gly ggc Gly 320 tac Tyr gcc Ala gcc ggc Gly ggC Giy caa Gin 305 gtc Val gt t Vai cgc Arg tac ctc Leu atc Ile 290 gtg Val ggc Gly ggt Gly tac Tyr gca PCTIBOOIOO923 tcc ggc 931 Ser Gly cac ggc 979 His Giy gag tcc Giu Ser cag cac Gin His 325 acc gac Thr Asp 340 ggc atc Gly Ile aag cgc kla Phe. Ala Tyr Ala Leu Lys Arg ;iy Tal tal jeu ~eu rg 652 Ser Ala Asp Arg Pro Glu Tyr Ala Giu Pro Lys Gly WO 01/00843 Ala Met Thr Tyr 145 Met Thr Phe Phe Lys 225 Ala Ile Gly Gly Gin 305 Val1 Val Arg Tyr His Giy Ala 130 Met Gin Leu His Pro 210 Ala Cys Asp Leu Ile 290 Val Giy Gly Tyr Ala 370 Thr 100 Thr Ala Ala His Asp 180 Ser Ile Met Gly Giu 260 Ser His Glu Gin Thr 340 Gly Lys Gin Ile Ala Asp 150 Ala Val1 Tyr Arg Giu 230 Gly Val Lys Thr Tyr 310 Al a Ala Ile Ala Val1 Ile Cys 135 Val Lys Asn Leu Glu 215 Arg Ser Giu His Arg 295 Ser His Giu Pro Lys 375 Ile Ala 120 Aia Ala Val1 Giu Leu 200 Phe Thr Asn Leu Gly 280 S er Ile Leu Ala Ala 360 Thr Leu Gly 125 Giu Asn Ser Trp Gly 205 Ser Asp Phe Pro Asn 285 Asn Asp Arg Gin His 365 Ala 110 Gin Cys Val1 Gly Thr 190 Pro Giu Val Ala Ala 270 Gly Ser Tyr Ala Tyr 350 Ala PCT/iBOOIOO923 Lys Arg His Gly Vai Val Tyr Arg 160 Ser Gly 175 Ala Thr His Pro Giu Ala Val Val 240 Asp Phe 255 Gly Giu Gin Ile Asp Gly Pro Gly 320 Thr Tyr 335 Leu Ala Phe Ala <210> 447 <211> 1231 <212> DNA <213> Corynebacteriun glutarnicum WO 01/00843 WO 0100843PCT/IBOO/00923 <220> <221> CDS <222> (101)..(1231) <223> FRXA00956 <400> 447 gccggtgcag gcacgtgggc tggggcgaaa gacgccggcg cgctgctgaa aattttagcg accatctcca cattccatta ctaaaggttt aaataggatc atg act gaa aaa Met Thr Giu Lys aac ttg ggc ggc tcc acg ctg ctg cct gca tac ttc ggt gaa Asn Leu Giy Gly Ser Thr Leu Leu Pro Ala Tyr Phe Gly Glu ttc ggc Phe Gly ggc cag ttc gtc gcg gaa tcc ctc Gly Gin Phe Val Ala Giu Ser Leu cct gct ctc gac Pro Ala Leu Asp cag ctg gag Gin Leu Giu gaa gaa ctc Giu Giu Leu aag gcc ttc Lys Ala Phe gtt gac gcg acc aac agc cca gag ttc Val Asp Ala Thr Asn Ser Pro Giu Phe 211 259 307 355 ggc ggC Gly Gly tac ctc cgc gat Tyr Leu Arg Asp tac Tyr ctc ggc cgc cca Leu Gly Arg Pro ccg ctg acc gaa Pro Leu Thr Glu tgc Cys tcc aac ctg cca Ser Asn Leu Pro gca ggc gaa ggc Ala Gly Giu Gly ggc ttt gcg cgg Gly Phe Ala Arg ttc ctc aag cgc Phe Leu Lys Arg gac ctc gtc cac ggc ggt gca cac aaa Asp Leu Val His Gly Gly Ala His Lys act aac Thr Asn 100 cag gtg atc Gin Val Ile atc atc gca Ile Ile Ala 120 cag gtg ctg ctt gcc aag cgc atg ggc Gin Val Leu Leu Ala Lys Arg Met Gly aaa acc cgc Lys Thr Arg 115 451 499 gag acc ggc gca Giu Thr Gly Ala ggC Gly 125 cag cac: ggc acc gcc acc gct ctc Gin His Gly Thr Ala Thr Ala Leu 130 gca tgt Ala Cys 135 gcg ctc atg ggc Ala Leu Met Giy ctc Leu 140 gag tgc gtt gtc Giu Cys Val Val atg ggc gcc aag Met Gly Ala Lys gac Asp 150 gtt gcc cgc cag Val Ala Arg Gin ccc aac gtc tac Pro Asn Val Tyr atg cag ctg cac Met Gin Leu His gcg aag gtc atc Ala Lys Val Ile gtg gaa tct ggt Val Glu Ser Gly ggc acc ctg aag Gly Thr Leu Lys gac gcc Asp Ala 180 gtg aat gaa Val Asn Giu ctg cgc gat tgg Leu Arg Asp Trp gca acc ttc cac Ala Thr Phe His gag tcc cac Giu Ser His 195 WO 01/00843 tac ctt ctc ggc acc ccc gcc ggc Tyr Leu Leu Gly Thr Pro Ala Gly 200 205 cgt gaa ttc cac aag gtg atc tct Arg Giu Phe His Lys Val Ile Ser 215 220 gag cgc acc ggc aag ctt ccc gac 4 Giu Arg Thr Gly Lys Leu Pro Asp' 230 235 ggc tcc aac gcc atc ggc atg ttc Gly Ser Asn Ala Ile Gly Met Phe 250 gta gag ctc gtc ggc gct gag cca Val Glu Leu Val Gly Ala Giu Pro 265 aag cac ggc gca acc atc acc aac Lys His Gly Ala Thr Ile Thr Asn 280 285 acc cgt tcc tac ctg atg cgc aac 1027 Thr Arg Ser Tyr Leu Met Arg Asn 295 300 tac tcc atc tcc gcc gga ctt gat 1075 Tyr Ser Ile Ser Ala Gly Leu Asp 310 315 gca cac ctg cac gcc acc ggc cgc 1123 Ala His Leu His Ala Thr Giy Arg 330 gcc gaa gcc ctc caa gca ttc cag1 1171 Ala Glu Ala Leu Gin Ala Phe Gin 345 atc ccc gca ctg gaa tcc tca cac 1219 Ile Pro Ala Leu Giu Ser Ser His 360 365 gcc aag acc gcc 1231 Ala Lys Thr Ala 375 <210> 448 <211> 377 <212> PRT <213> Corynebacterium giutaxnicum <400> 448 cca Pro gcc Ala gtc Val 240 ttc Phe gaa Glu atc Ile Gly ggc Gly 320 tac Tyr gcc Ala gcc t tc Phe aag Lys 225 gcc Ala att Ile ggC Gly ggc Gly caa Gin 305 gtc Val gtt Val cgc Arg tac PCTfBOO/00923 atc gtg 739 Ile Val atg cta 787 Met Leu ggt ggt 835 Gly Gly 245 gaa ggt 883 Glu Gly 260 tcc ggc 931 Ser Gly cac ggc 979 His Gly gag tcc Glu Ser cag cac Gin His 325 acc gac Thr Asp 340 ggc atc Gly Ile aag cgc Ala Phe Ala Tyr Ala Leu Lys Arg 370 WO 01/00843 WO 0100843PCT/IBOO/00923 Met Thr Giu Lys Glu Asn Leu Gly Gly Ser Thr Leu Leu Pro Ala Tyr 1 5 10 Phe Gly Leu Asp Phe Arg Thr Pro Gly Phe Ala His Met Giy Thr Ala 130 Tyr Met 145 Met Gin Thr Leu Phe His Phe Pro 210 Lys Ala 225 Ala Cys Ilie Asp Gly Leu Gly Ile 290 Gln Val 305 Gi u Gl n Glu Leu Ala Lys Lys 115 Thr Gly Leu Lys Giu 195 Thr Gin Va I Asp Asp 275 Leu Giu Phe Leu Glu Thr Arg Thr 100 Thr Ala Ala His Asp 180 S er Ile Met Gly Glu 260 Ser His Glu Gly Gly Giu Lys Leu Gly Glu Cys 70 Ile Phe Asn Gin Arg Ile Leu Ala Lys Asp 150 Giy Ala 165 Ala Vai His Tyr Vai Arg Leu Giu 230 Gly Gly 245 Gly Val Giy Lys Gly Thr Ser Tyr 310 Gin Ala Giy 55 Ser Leu Val Ile Cys 135 Val Lys Asn Leu Giu 215 Arg Ser Giu His Arg 295 Ser Phe Val 25 Phe Vai 40 Tyr Leu Asn Leu Lys Arg Ile Gly 105 Ala Giu 120 Ala Leu Aia Arg Val Ile Giu Ala 185 Leu Gly 200 Phe His Thr Gly Asn Ala Leu Val 265 Gly Ala 280 Ser Tyr Ile Ser Ala Asp Arg Pro Giu 90 Gin Thr Met Gin Pro 170 Leu Thr Lys Lys Ile 250 Gly Thr Leu Ala Giu Ala Asp Leu 75 Asp Val1 Gly Gly Gin 155 Val1 Arg Pro Val Leu 235 Gly Ala Ile Met Gly 315 Ser Thr Tyr Ala Leu Leu Ala Leu 140 Pro Giu Asp Ala Ile 220 Pro Met Giu Thr Arg 300 Leu Gly Leu Leu Asn Ser Leu Gly Gly Glu Val His Leu Ala 110 Gly Gin 125 Giu Cys Asn Val Ser Gly Trp Thr 190 Gly Pro 205 Ser Glu Asp Val Phe Ala Pro Ala 270 Asn Gly 285 Asn Ser Asp Tyr Arg Ala Pro Pro Arg Gly Gly Lys His Val Tyr Ser 175 Ala His Giu Val Asp 255 Gly Gin Asp Pro Thr Ala Giu Pro Lys Gly Arg Gly Val1 Arg 160 Gly Thr Pro Ala Val 240 Phe Glu Ile Gly Gly 320 Tyr Vai Giy Pro Gin His Ala His Leu His Ala Thr WO 01/00843 WO 0100843PCTIiBOO0923 325 Val Gly Ilie Thr ASP Ala Giu Ala Leu 340 345 Arg Tyr Giu Gly Ile Ile Pro Ala Leu 355 360 Tyr Ala Leu Lys Arg Ala Lys Thr Ala 370 375 330 Gin Ala Phe Gin Tyr Leu Ala 350 Ala Phe Ala Giu Ser Ser <210> 449 <211> 1401 <212> DNA <213> Corynebacterium glutamricun <220> <221> <222> <223>

CDS

(101)..(1378) RXA0 0064 <400> 449 tcccctaaaa cgtctagagt agtggcttga ggtcactgct ctttttttgt gccctttttt ggtccgtcta ttttgccacc acatgcggag gtacgcagtt atg agt tca gtt Met Ser Ser Val 1 ctg cag gat ttt Leu Gin Asp Phe gca gag cga att Ala Glu Arg Ile ttg ttc cac gag Leu Phe His Giu gac att Asp Ile aag cgc aag Lys Arg Lys act cgc ggt Thr Arg Gly gat gag ctc aag Asp Glu Leu Lys aaa aat ctg aag Lys Asn Leu Lys ctg gat ctt Leu Asp Leu gat gag ttg Asp Glu Leu aag cct tcg tcg Lys Pro Ser Ser cag ttg gat ttc Gin Leu Asp Phe ttg gcg Leu Ala ttg cct ggt aag Leu Pro Gly Lys gat ttc aag gct Asp Phe Lys Ala gat ggt act gat Asp Gly Thr Asp gtc Val cgt aac tat ggc Arg Asn Tyr Gly ctg gat ggc atc Leu Asp Gly Ile gtt gat att cgc cag att Val Asp Ilie Arg Gin Ilie 80 tgg gcg gat ttg Trp Ala Asp Leu ggt gtt cct gtg Gly Val Pro Val cag gtc ttg gcg Gin Val Leu Ala ggg gat Gly Asp 100 gct tcg agc Ala Ser Ser ttc ggt aac Phe Gly Asn 120 ttg Leu 105 aac atc atg ttt Asn Ile Met Phe gtg atc agc tgg Val Ile Ser Trp, tcg tac att Ser Tyr Ile 115 gaa acc gtt Glu Thr Val aat gat tcg gtt Asn Asp Ser Val cct tgg tcg aag Pro Trp Ser Lys gaa Glu 130 WO 01/00843 aag tgg att tgc cct gtt ccg ggc tat gat cgc cat ttc tcc Lys Trp Ile Cys Pro Vai Pro Gly Tyr Asp Arg His Phe Ser 135 140 145 gag cgt Giu Arg 150 cct gat Pro Asp ggc atg Gly Met aca gaa Thr Giu gac ttc Asp Phe 215 gat gaa Asp Giu 230 ggc aac Gly Asn ctc gcg Leu Ala aag tgg Lys Trp gtc aat 1027 Val Asn 295 cgc gcg 1075 Arg Ala 310 aag gtt 1123 Lys Val cag tgg 1171 Gin Trp ttc Phe atg Met tgg Trp, gac Asp 200 cgc Arg ttC Phe ccg Pro ggt Gly tac Tyr 280 cag Gin gtg Val1 ctg Leu act Thr cca Pro 160 aag Lys ccg Pro atg Met gcc Al a ggg Giy 240 tc t Ser acc Thr ggc Gly ggc Gly ttg Leu 320 gct Ala att Ile a tg Met aat Asn act Thr gaa Giu gtt Val1 225 ctt Leu act Thr tct Ser att Ile gat Asp 305 gct Aia gag Giu tcc Ser gaa Giu cag Gin ttc Phe 195 gca Ala acg Thr gag Giu aag Lys gag Giu 275 cc t Pro gag Giu aag Lys ggt Gly gat Asp 355 PCTIBOOIOO923 atc acg 547 Ile Thr gac ggc 595 Asp Giy 165 gtt aag 643 Vai Lys 180 acg gtg 691 Thr Vai gct ccg 739 Ala Pro ctg acc 787 Leu Thr gcc gct 835 Ala Ala 245 atc act 883 Ile Thr 260 aac cgc 931 Asn Arg aac aag 979 Asn Lys gga gtg Gly Val ttc aac Phe Asn 325 gtc gcg Val Ala 340 gtg gtt Vai Val WO 01/00843 cct ggt acg 1219 Pro Gly Thr 360 gcg ttg acg 1267 Ala Leu Thr 375 aac aaa aat 1315 Asn Lys Asn 390 gag gtt gcc 1363 Giu Val Ala gag cat tac 1401 Giu His Tyr tct cgc gtg gct gag ttg gct ai Ser Arg Val Ala Glu Leu Ala L] 365 gcg ggt tct tct tac ccg ctg cc Ala Gly Ser Ser Tyr Pro Leu Ai 380 3 cgt ttg gca ccg tcg ctg cct C( Arg Leu Ala Pro Ser Leu Pro Pi 395 400 gat ggc gtg gct acc tgt gtg ci Asp Gly Val Ala Thr Cys Val D~ 410 415 aac taaaagtgaa tacagcggag aca Asn gcc Ala gat Asp gag Glu gca Ala pCTIIBOOIOO923 ggc atc Gly Ile ccg gag Pro Giu gaa ctt Glu Leu 405 gca gcg Ala Ala 420 <210> 450 <211> 426 <212> PRT <213> Corynebacteriun glutamicun <400> 450 Met Ser Ser Val Ser Leu Gin Asp Phe 1 Phe Leu Phe Ala Asp Val Ser Lys His 145 His Lys Ala Asp Ile Leu Trp, Giu 130 Phe Glu Leu Asp Gly Arg Ala Ser 115 Glu Ser Asp Asp Glu Thr Gln Gly 100 Tyr Thr Ile 5 Ile Leu Leu Asp Ile Asp Ile Val Thr Arg Arg Ala 55 Arg Ala S er Gly Trp 135 Arg Lys Gly 40 Leu Asn Asp Ser Asn 120 Ile Phe Asp 10 Asp Pro Gly Gly Leu 90 Asn Asp Pro Phe Gi u Leu Ser Gly Leu Val1 Met Val Pro 140 Met Arg Lys Glu Asp Asp Pro Phe Gin 125 Gly Ile Gly Lys Leu Lys Ile Glu Val Trp Asp Val WO 01/00843 PCT/IB00/00923 Met Asn Glu Asp Gly Pro Asp Met Asp Ala Val Glu Glu Leu Val Lys 165 170 175 Asn Pro Gin Val Lys Gly Met Trp Val Val Pro Val Phe Ser Asn Pro 180 185 190 Thr Gly Phe Thr Val Thr Glu Asp Val Ala Lys Arg Leu Ser Ala Met 195 200 205 Glu Thr Ala Ala Pro Asp Phe Arg Val Val Trp Asp Asn Ala Tyr Ala 210 215 220 Val His Thr Leu Thr Asp Glu Phe Pro Glu Val Ile Asp Ile Val Gly 225 230 235 240 Leu Gly Glu Ala Ala Gly Asn Pro Asn Arg Phe Trp Ala Phe Thr Ser 245 250 255 Thr Ser Lys Ile Thr Leu Ala Gly Ala Gly Val Ser Phe Phe Leu Thr 260 265 270 Ser Ala Glu Asn Arg Lys Trp Tyr Thr Gly His Ala Gly Ile Arg Gly 275 280 285 Ile Gly Pro Asn Lys Val Asn Gin Leu Ala His Ala Arg Tyr Phe Gly 290 295 300 Asp Ala Glu Gly Val Arg Ala Val Met Arg Lys His Ala Ala Ser Leu 305 310 315 320 Ala Pro Lys Phe Asn Lys Val Leu Glu Ile Leu Asp Ser Arg Leu Ala 325 330 335 Glu Tyr Gly Val Ala Gin Trp Thr Val Pro Ala Gly Gly Tyr Phe Ile 340 345 350 Ser Leu Asp Val Val Pro Gly Thr Ala Ser Arg Val Ala Glu Leu Ala 355 360 365 Lys Glu Ala Gly Ile Ala Leu Thr Gly Ala Gly Ser Ser Tyr Pro Leu 370 375 380 Arg Gin Asp Pro Glu Asn Lys Asn Leu Arg Leu Ala Pro Ser Leu Pro 385 390 395 400 Pro Val Glu Glu Leu Glu Val Ala Met Asp Gly Val Ala Thr Cys Val 405 410 415 Leu Leu Ala Ala Ala Glu His Tyr Ala Asn 420 425 <210> 451 <211> 1143 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1120) WO 01/00843 <223> RXN00448 <400> .451 ccatttacct tcaactaagg tagccgtaac tgcaaagctc catagagata accgtagtag gtatgtgcca cacttgtcag PCT/IBOO/00923 aggccatcct cttcagtgtt att Ile ctc Leu cgc Arg tcc Ser ctc Leu gcc Ala gta Val cgt Arg agc Ser ttc Phe 150 atc Ile gtc Val cat His tcc Ser ctc Leu tca Ser gcc Ala atc Ile gtc Val1 aaa Lys tat Tyr gca Ala 135 gac Asp tgg Trp cca Pro tta Leu cca Pro gac Asp tcC Ser ctt Leu ctc Leu acc Thr gcc Al a 105 gga Gly atg Met ctt Leu gac Asp cga Arg 185 cac His gta Val ctc Leu ggc Gly gaa Glu gcg Ala c ac His gtc Val tcc Ser gac Asp t tc Phe gtc Val 170 gtt Val atc Ile tgc Cys cat His gct Ala gca Ala gtc Val1 75 gca Ala tac Tyr cac His gga Gly gac Asp 155 gtc Val1 ggc Gly c tg Leu atc Ile gca Al a aaa Lys acc Thr 60 ccc Pro c ca Pro gac Asp ccc Pro c tg Leu 140 ggC Gly caa Gin cca Pro gct Ala g tg Val 1 ctc Leu gtc Val gaa Glu gcc Ala gat Asp ac c Thr cgc Arg gcc Al a gt a Val 145 tcc Ser gtg Val1 gca Ala atc Ile act acc aaa Thr Thr Lys ggc Gly ggc Gly ttc Phe gaa Glu ctt Leu gtc Val atg Met 1.15 tcc Ser gtg Val tgg Trp gct Ala cga Arg 195 ggt Gly gga Gly tac Tyr gac Asp gat Asp c tc Leu gta Val1 100 caa Gin Gly gtc Val1 atc Ile gaa Glu 180 gtg Val gac Asp gac Asp tcc Ser aac Asn 4tt Val gcg Ala gac Asp tcc Ser cac His tgg Trp acc Thr agc Ser 165 gt t Val tct Ser aac Asn WO 01/00843 PCTIIBOO/00923 gcc gct ggc agc tac cgc gac tcc 787 Ala Ala Gly Ser Tyr Arg Asp Ser 225 ggt Gly ac c Thr 230 agc Ser ctc Leu gca Al a gtt Vai gcc Al a gaa Giu gcc Ala 280 cag Gin cca Pro atc ctg tct ctc tct tta Leu Ser Leu Ser Leu 220 gca ggc acc gac cca Ala Giy Thr Asp Pro 235 ggc cca ctg gtc aaa Gly Pro Leu Val Lys 250 gcc cgc gaa ggc ctc Ala Arg Giu Gly Leu 265 gac aac ggc tac cga Asp Asn Giy Tyr Arg 285 cga cgc gcc aaa gaa Arg Arg Ala Lys Giu 300 gtg ctc cgt ctc cac Vai Leu Arg Leu His 315 cac gct gaa acc ctc caa cru Gin Leu tcc ggc 1027 Ser Gly 295 tcc agg 1075 Ser Arg 310 cag ctc 1120 ctc Leu c tc Leu 255 gca Ala cgc Arg gtt Val ggc Gly gca gtc Val 240 gac Asp gaa Giu atc Ile agc Ser aca Thr 320 cgg cgc Arg gaa Glu cag Gin cgc Arg cct Pro 305 cca Pro atc Gin Leu Ile His Ala Giu Thr Leu Giy Ala Arg Ile Giu Val Phe tagttttatc ggctgatgat tct 1143 <210> 452 <211> 340 <212> PRT <213> Corynebacterium giutanicum <400> 452 Val Thr Thr Lys Asp Ile Ser Arg I 1 5 Leu Ile Gly Gly Ser Leu Leu Arg .1 Val Phe Gly Tyr Asn Arg Ser Arg Giu Gly Phe Asp Val Ser Ala Asp I Ala Ala Giu Asp Ala Leu Ilie Val I 70 Asp Ser Leu Leu Asp Ala Val His Val1 10 Leu Gly Gi u Ala His Leu His Val Arg Ala Gly Giy Ser Asp Ala Ile Phe WO 01/00843 WO 0100843PCT/IBOO/00923 90 Thr Asp Val Val Ser Val Lys Thr Ala Val Tyr Asp Ala Val Lys Ala 100 105 110 Arg Asn Met Gin His Arg Tyr Val Gly Ser His Pro Met Ala Gly Thr 115 120 125 Ala Asn Ser Gly Trp Ser Ala Ser Met Asp Gly Leu Phe Lys Arg Ala 130 135 140 Val Trp Val Val Thr Phe Asp Gin Leu Phe Asp Gly Thr Asp Ile Asn 145 150 155 160 Ser Thr Trp Ile Ser Ilie Trp, Lys Asp Val Val Gin Met Ala Leu Ala 165 170 175 Val Gly Ala Glu Val Val Pro Ser Arg Val Gly Pro His Asp Ala Ala 180 185 190 Ala Ala Arg Val Ser His Leu Thr His Ile Leu Ala Glu Thr Leu Ala 195 200 205 Ile Val Gly Asp Asn Gly Gly Ala Leu Ser Leu Ser Leu Ala Ala Gly 210 215 220 Ser Tyr Arg Asp Ser Thr Arg Val Ala Gly Thr Asp Pro Gly Leu Val 225 230 235 240 Arg Ala Met Cys Glu Ser Asn Ala Gly Pro Leu Vai Lys Ala Leu Asp 245 250 255 Glu Ala Leu Ala Ile Leu His Giu Ala Arg Giu Gly Leu Thr Ala Glu 260 265 270 Gin Pro Asn Ile Glu Gin Leu Ala Asp Asn Gly Tyr Arg Ser Arg Ile 275 280 285 Arg Tyr Giu Ala Arg Ser Gly Gin Arg Arg Ala Lys Giu Ser Val Ser 290 295 300 Pro Thr Ilie Thr Ser Ser Arg Pro Val Leu Arg Leu His Pro Gly Thr 305 310 315 320 Pro Asn Trp, Glu Lys Gin Leu Ilie His Ala Glu Thr Leu Gly Ala Arg 325 330 335 Ile Giu Val Phe 340 <210> 453 <211> 689 <212> DNA <213> Corynebacteriun glutarnicum <220> <221> CDS <222> <223> FRX.A00448 WO 01/00843 <400> 453 tat gtg gq Tyr Val GI 1 gca tcc at Ala Ser ME gac cag ct Asp Gin Le tgg aaa gz Trp Lys A~c cca tcc c Pro Ser Ai tta aca ci Leu Thr Hj ggc gca ct Gly Ala L( cgc gtt g( Arg Val A] 1] aac gcc g5 Asn Ala G] 130 cac gaa gc His Giu A] 145 ctt gcc gi Leu Ala A., ggc cag c5 Gly Gin Ai agg cca gt Arg Pro Vi 1! ctc atc cz PCT/EB00100923 tcc cac ccc atg Ser His Pro Met gca ggc acc gcc aac tcc ggc Ala Gly Thr Ala Asn Ser Gly gac Asp t tc Phe gtc Val gt t Val1 atc Ile tct Ser 100 ggc Gly cca Pro cgc Arg aac Asn cgc Arg 180 ctc Leu gct ctg Leu ggc Gly caa Gin cca Pro 70 gc t Ala tct Ser gac Asp gtc Val ggc Gly 150 tac Tyr aaa Lys ctc Leu acc aaa Lys gac Asp gca Ala ga t Asp ac c Thr gcc Ala gga Gly 120 gcc Ala ac C Thr tcc Ser tcc Ser ccg Pro 200 ggc Gly cga Arg atc Ile ctc Leu gca Ala ctc Leu gc t Ala 105 C tC Leu C tC Leu gca Ala cgc Arg gtt Val 185 ggc Gly gca Ala gca Ala aac Asn gcc Ala gca Ala gcc Ala ggc Gly gtc Val gac Asp gaa Giu 'atc Ile 170 agc Ser aca Thr cgg Arg tgg Trp acc Thr ggc Gly gca Ala gtc Val tac Tyr gcc Ala gca Ala 140 cca Pro tac Tyr acc Thr aac Asn gaa Glu 220 agc Ser t tC Phe atc Ile gtc Val cat His ggt Gly ac c Thr agc Ser C tC Leu caa Gin 160 tcc Ser tcc Ser cag Gin 48 96 144 192 240 288 336 384 432 480 528 576 624 Leu Ile His Ala Glu Thr Leu 210 215 tagttttatc ggctgatgat tct 689 <210> 454 WO 01/00843 <211> 222 <212> PRT <213> Corynebacteriun giutamicum <400> 454 Tyr Val Gly Ser His Pro Met Ala Gly PCTIBOO/00923 1 Ala Asp Trp, Pro Leu Gly Arg Asn His 145 Leu Gly Arg Leu Ser Gin Lys Ser Thr Ala Val Al a 130 Giu Al a Gin Pro Ile 210 5 Gly Asp Val1 Gly Leu Leu Thr Leu Glu Gly 165 Ala Arg Leu Giy Gin Pro '70 Ala Ser Asp Val Gly 150 Tyr Lys Leu Phe Thr Met 55 His Giu Leu Pro Lys 135 Leu Arg Giu His Lys Asp Ala Asp Thr Ala Gly 120 Ala Thr S er Ser Pro 200 Arg Ile Leu Ala Leu Ala 105 Leu Leu Ala Arg Val 185 Gly Thr 10 Al a Asn Ala Ala Al a 90 Giy Val Asp Giu Ile 17 0 Ser Thr Ala Vai Ser Val1 Ala 75 Ile Ser Arg Giu Gin 155 Arg Pro Pro Asn Trp, Thr Gly Ala Val Tyr Ala Ala 140 Pro Tyr Thr Asn Ser Val1 Trp Ala Arg Gly Arg Met 125 Leu Asn Giu Ile Trp 205 Gly Val Ile Giu Val Asp Asp 110 Cys Ala Ile Ala Thr 190 Glu Trp Thr Ser Val Ser Asn Ser Giu Ile Glu Arg 175 Ser Lys Ser Phe Ile Val His Gly Thr S er Leu Gin 160 Ser Ser Gin Ala Giu Thr Leu Gly Ala Arg Ile Giu Val Phe <210> 455 <211> 346 <212> DNA <213> Corynebacteriun giutanicum <22 0> <221> CDS <222> (346) <223> FRXA00452 <400> 455 ccatttacct tcaactaagg tagccgtaac tgcaaagctc aggccatcct cttcagtgtt WO 01/00843 catagagata accgtagtag gtatgtgcca cacttgtcag att tcc cgc cca gta tgc atc ctg ggc ctc ggc Ile Ser Arg Pro Val Cys Ile Leu Gly Leu Gly 15 ctc ctc cgc gac ctc cat gca gcc aac cac tcc Leu Leu Arg Asp Leu His Ala Ala Asn His Ser 30 cgc tca cgc tcc ggc gct aaa tca gcc gtc gac Arg Ser Arg Ser Gly Ala Lys Ser Ala Val Asp 45 tcc gcc gat ctt gaa gca acc ctc cag cgt gca Ser Ala Asp Leu Glu Ala Thr Leu Gin Arg Ala ctc atc gtc ctc gcg gtc ccc atg acc gca atc Leu Ile Val Leu Ala Val Pro Met Thr Ala Ile 75 <210> 456 <211> 82 <212> PRT <213> Corynebacteriun glutaxnicum <400> 456 Val Thr Thr Lys Asp Ile Ser Arg Pro Val Cys 1 5 10 Leu Ile Giy Gly Ser Leu Leu Arg Asp Leu His 25 Val Phe Gly Tyr Asn Arg Ser Arg Ser Gly Ala 40 Giu Gly Phe Asp Val Ser Ala Asp Leu Glu Ala 55 Ala Ala Giu Asp Ala Leu Ile Val Leu Ala Val 70 75 Asp Ser act Thr atc Ile t tc Phe ggc Gly gcc Ala tcg Ser PCTABOO/00923 acc aaa gac 115 Thr Lys Asp ggc gga tcc 163 Gly Gly Ser ggc tac aac 211 Gly Tyr Asn ttc gac gtt 259 Phe Asp Val gaa gat gcg 307 Giu Asp Ala 346 Ile Leu Gly Leu Gly Ala Ala Asn His Ser Lys Ser Ala Val Asp Thr Leu Gin Arg Ala Pro Met Thr Ala Ile <210> <211> <212> <213> <220> <221> <222> <223> <400> 457 1248

DNA

Corynebacteriun glutarnicum

CDS

(101)..(1225) RXA0 0584 457 WO 01/00843 WO 0100843PCT[IBOO/00923 tagttgtgcc acctaaaacg cgaacagaac cggagtcgag cagcacctcc ccgcaagggt agaggggctg cttttttgtt tcctaaattc accccatccc atg cat agc Met His Ser 1 ctc gaa aac gcg Leu Giu Asn Ala cct gaa Pro Giu gcg tca Ala Ser agg caa gaa aaa atg agt tct cca gtc Arg Gin Giu Lys Met Ser Ser Pro Val tca Ser acc agc aac Thr Ser Asn cgc gtc gtg gct Arg Vai Val Ala t tc Phe cac gag ctg cct agc cct aca His Giu Leu Pro Ser Pro Thr gat ctc Asp Leu gag cag Glu Gin atc gcc gca aac cca ctg Ilie Ala Ala Asn Pro Leu aca cca aag cag Thr Pro Lys Gin tcc aag gtg Ser Lys Val 259 gat cgc cag gac Asp Arg Gin Asp gct gat atc ttc Ala Asp Ile Phe ggc gac gat gac Gly Asp Asp Asp cgc Arg ctc gtt gtc gtt Leu Val Val Val gga cct tgc tca gtt cac gat cct gaa Gly Pro Cys Ser Val His Asp Pro Giu 80 gca Ala gcc atc gat tac Aia Ile Asp Tyr aac cgc ctg gct Asn Arg Leu Ala ctg gca aag cgc Leu Aia Lys Arg ctt gat Leu Asp 100 cag gac ctc Gin Asp Leu atc gtc gga Ile Vai Gly 120 -aag Lys 105 att gtc atg cgc Ile Val Met Arg gtg Val 110 tac ttc gag aag Tyr Phe Giu Lys cct cgc acc Pro Arg Thr 115 aac gaa acc Asn Glu Thr tgg aag gga ttg Trp Lys Gly Leu atc Ile 125 aat gat cct cac Asn Asp Pro His tac gac Tyr Asp 135 atc cca gag ggc Ile Pro Giu Gly cgc att gcg cgc Arg Ilie Ala Arg gtg ctt atc gac Val Leu Ile Asp 355 403 451 499 547 595 643 691 739 gtt Vai 150 gtg aac ctt gat Val Asn Leu Asp cca gtc ggc tgc Pro Val Gly Cys gaa Giu 160 ttc ctc gaa cca Phe Leu Giu Pro agc cct cag tac Ser Pro Gin Tyr gcc gac act gtc Ala Asp Thr Val gca Ala 175 tgg gga gca atc Trp Gly Ala Ile ggc gct Gly Ala 180 cgt acc acc Arg Thr Thr atg cca att Met Pro Ile 200 tct cag gtg cac Ser Gin Val His cgc Arg 190 cag ctg gct tct Gin Leu Ala Ser ggg atg tct Gly Met Ser 195 cag gtt gca Gin Val Ala ggt ttc aag aac Gly Phe Lys Asn gga Gly 205 act gac gga aac Thr Asp Gly Asn gtc gac Val Asp 215 gcg gta cag gct Ala Val Gin Ala gcc Ala 220 cag aac cca cac Gin Asn Pro His ttc ttc gga acc Phe Phe Gly Thr WO 01/00843 WO 0100843PCTIBOOIOO923 tcc gac gac ggc gcg ctg agc gtc gtg gag acc gca ggc aac agc aac 835 Ser Asp Asp Gly Ala Leu Ser Val Vai Giu Thr Ala Gly Asn Ser Asn 230 235 240 245 tcc cac atc att ttg cgc ggc ggt acc tcc ggc ccg aat cat gat gca 883 Ser His Ile Ilie Leu Arg Gly Gly Thr Ser Gly Pro Asn His Asp Ala 250 255 260 gct tcg gtg gag gcc gtc gtc gag aag ctt ggt gaa aac gct cgt ctc 931 Ala Ser Val Giu Ala Val Val Glu Lys Leu Gly Glu Asn Ala Arg Leu 265 270 275 atg atc gat gct tcc cat gct aac tcc ggc aag gat cat atc cga cag 979 Met Ilie Asp Ala Ser His Ala Asn Ser Gly Lys Asp His Ile Arg Gin 280 285 290 gtt gag gtt gtt cgt gaa atc gca gag cag att tct ggc ggt tct gaa 1027 Val Glu Val Val Arg Giu Ile Ala Giu Gin Ile Ser Gly Gly Ser Giu 295 300 305 gct gtg gct gga atc atg att gag tcc ttc ctc gtt ggt ggc gca cag 1075 Ala Vai Ala Gly Ilie Met Ile Glu Ser Phe Leu Val Gly Giy Ala Gin 310 315 320 325 aac ctt gat cct gcg aaa ttg cgc atc aat ggc ggt gaa ggc ctg gtg 1123 Asn Leu Asp Pro Ala Lys Leu Arg Ilie Asn Gly Giy Giu Giy Leu Val 330 335 340 tac gga cag tct gtg acc gat aag tgc atc gat att gac acc acc atc 1171 Tyr Giy Gin Ser Val Thr Asp Lys Cys Ile Asp Ile Asp Thr Thr Ile 345 350 355 gat ttg ctc gct gag ctg gcc gca gca gta agg gaa cgc cga gca gca 1219 Asp Leu Leu Ala Glu Leu Ala Ala Ala Val Arg Giu Arg Arg Ala Ala 360 365 370 gcc aag taattaaggg cgctagactg tta 1248 Ala Lys 375 <210> 458 <211> 375 <212> PRT <213> Corynebacteriun giutamicun <400> 458 Met His Ser Pro Giu Arg Gin Giu Lys Met Ser Ser Pro Val Ser Leu 1 5 10 Glu Asn Ala Ala Ser Thr Ser Asn Lys Arg Val Val Ala Phe His Glu 25 Leu Pro Ser Pro Thr Asp Leu Ile Ala Ala Asn Pro Leu Thr Pro Lys WO 01/00843 WO 0100843PCTIBOOIOO923 Gin Ala His Ala.

Giu His Lys 145 Phe Gly Ala Asn Phe 225 Ala Pro Giu Asp Ser 305 Val Gly Ile Ala Gly Asp Lys Lys Leu 130 Val1 Leu Ala Ser Ile 210 Phe Gly Asn Asn His 290 Gly Gly Giu Asp Giu Arg 70 Ala Gin Ile Tyr Val1 150 Ser Arg Met Val1 Ser 230 Ser Ala Met Val Ala 310 Asn Tyr Asp Gin Leu Ile Asp Val1 Asp 135 Val1 Pro Thr Pro Asp 215 Asp His Ser Ile Glu 295 Val Leu Gly Leu Asp Val1 Asp Leu Gly 120 Ile Asn Gin Thr Ile 200 Ala Asp Ile Val Asp 280 Val Ala Asp Gin Leu Gin Asp Val Val 75 Ala Asn Ile Val Lys Giy Giu Giy Asp Leu 155 Tyr Ala 170 Ser Gin Phe Lys Gin Ala Ala Leu 235 Leu Arg 250 Ala Vai Ser His Arg Giu Ile Met 315 Ala Lys 330 Val Thr Glu Leu Asp Cys Aia Val1 110 Asn Ile Gly Val1 Arg 190 Thr Asn Val1 Thr Lys 270 Ser Giu Ser Ile Cys 350 Ala Ile Ser Pro Tyr Asp Ala Cys Ala 175 Gin Asp Pro Giu Ser 255 Leu Giy Gin Phe Asn 335 Ile Val Phe Val1 Leu Phe Pro Arg Giu 160 Trp Leu Giy His Thr 240 Gly Giy Lys Ile Leu 320 Gly Asp Arg 365 WO 01/00843 WO 0100843PCTIIBOO/00923 Glu Arg Arg Ala Ala Ala Lys 370 375 <210> <211> <212> <213> <220> <221> <222> <223> 459 1983

DNA

Corynebacterium

COS

(101) (1960) RXA00579 glut aric ur <400> 459 tcgtctaagt ttttctttga gttttcatat gtagaaggca tcgtcggctt cggcctggcg gtgcttttct cgttgttttg tggttttgtc agaggatgtc atg cgc gtt tta Met Arg Vai Leu att gat aat tat Ile Asp Asn Tyr tct ttc acg ttt aat ctc gcc acc tat Ser Phe Thr Phe Asn Leu Ala Thr Tyr gtg gaa Val Giu gag gtt acg Glu Val Thr gat gag atg Asp Glu Met ggt Giy cag gca cct gtg Gin Ala Pro Val gtg cct aat gat caa gaa ata Val Pro Asn Asp Gin Glu Ile ctt ttc gac gcc Leu Phe Asp Ala atc ctc tca cct Ile Leu Ser Pro ggC Gly ccg ggc cac Pro Gly His gcc ggc Ala Gly gtt gcg gct gat Val Ala Ala Asp ggt atc tgt gca Gly Ile Cys Ala ggc Gly gtc att gag cgt Val Ile Giu Arg gca Ala cgc gtt ccg att Arg Val Pro Ile ttg ggt Leu Gly gtg tgt tta Val Cys Leu cac cag ggc att His Gin Gly Ile ttg gcc tat ggc Leu Ala Tyr Gly gat gtt gat ttg Asp Val Asp Leu ccc agg ccg gtc Pro Arg Pro Val cac ggt His Gly 100 gag gtt tcg Giu Val Ser cct gaa acg Pro Giu Thr 120 cag Gln 105 atc acc cat gat Ile Thr His Asp tca ggt tta ttt gca ggc atc Ser Gly Leu Phe Ala Gly Ile 115 ttt gag gcg gtg Phe Giu Ala Val tat cac tcg atg Tyr His Ser Met gtg Val 130 gca acc cgc Ala Thr Arg ttg ccg Leu Pro 135 gag tca ttg aaa Glu Ser Leu Lys aca gct acc agc Thr Ala Thr Ser ga t Asp 145 gat ggt ttg atc Asp Gly Leu Ile atg Met

ISO

gca ttg gca cat Ala Leu Ala His gtg ctt ccg cag Val Leu Pro Gin ggt gtg caa ttt Gly Val Gin Phe WO 01/00843 WO 0100843PCT/iBOO/00923 ccg gaa Pro Glu ctt aat Leu Asn ccg ctc Pro Leu tcc tcc Ser Ser 215 ggt gat Gly Asp 230 gag ggg Giu Gly gtt gcg Val Ala tat gag Ty'r Giu ctt ccg 1027 Leu Pro 295 tcg gat 1075 Ser Asp 310 gaa gaa 1123 Giu Giu gcg tcc 1171 Ala Ser ctc gac 1219 Leu Asp tat gaa 1267 tct att ggt Ser Ile Gly 170 tta gcg cgc Leu Ala Arg 185 agc gtt gat Ser Val Asp 200 cat gct ttt His Ala Phe gcc agc ggt Ala Ser Gly gat ttc ttc Asp Phe Phe 250 ccc ggt caa Pro Gly Gin 265 ctt aaa gcg Leu Lys Ala 280gat gcg cac Asp Ala His cag gtt cgg Gin Val Arg acc atc aag Thr Ile Lys 330 gga cac ctc Gly His Leu 345 aaa att cgc Lys Ile Arg 360 atc tgc ctg gga caa ttc Gly Gin Phe aca Thr tca Ser tgg Trp, cct Pro 235 acc Thr ggt Gly gaa Glu ctc Leu ttg Leu 315 aag Lys gc t Ala aga Arg acc tat Tyr gca Ala ctc Leu 220 ctc Leu tgg Trp ttt Phe gct Ala att Ile 300 c tg Leu ctg Leu t tg Leu gcc Ala aca cgc Arg gcg Ala 205 gat Asp gca Ala cta Leu cgt Arg ggc Gly 285 ttt Phe gcg Ala cat His cag Gin cag Gin 365 aaa ggc Giy tgg Trp 190 gt t Val1 gat Asp cgc Arg aag Lys ct t Leu 270 gca Ala gcc Ala ttg Leu aat Asn gtt Vai 350 gag Giu ctt cat cag atc att aag His Gin Ile Ile Lys 175 caa Gin t tt Phe gcc Ala aca Thr gag Giu 255 ggc Gly cgg Arg gat Asp ggg Gly ct t Leu 335 cga Arg ctg Leu cag Ctc Leu gaa Glu caa Gin aaa Lys 240 gat Asp tgg Trp, gc t Ala cgc Arg gag Glu 320 gtc Val gat Asp att Ile ggc gag Giu t tc Phe 210 acc Thr cat His gcc Ala ggt Gly cac His 290 atc Ile gac Asp ccg Pro aaa Lys cgc Arg 370 act aaa Lys 195 ttt Phe agc Ser aat Asn gcc Ala tac Tyr 275 act Thr gca Ala gag Giu cgg Arg gat Asp 355 ggc Giy gat aac Asn 180 act Thr gc c Ala tat Tyr gtc Val1 aac Asn 260 gtt Val tcg Ser gtg Val1 tgg Trp, ata Ile 340 gag Giu gaa Giu gtg t tc Phe att Ile cat His ctt Leu ggc Gly 245 tca Ser ggt Gly ag t Ser gaa Giu ttt Phe 325 cct Pro tat Tyr tcg Ser gcc 643 691 739 787 835 883 931 979 Tyr Glu Ile Cys Leu Thr Thr Lys Leu Gin Gly Thr Thr Asp Val Ala WO 01/00843 WO 0100843PCTIBOO/00923 375 cct ctg 1315 Pro Leu 390 ggt gcg 1363 Gly Ala gag cgg 1411 Glu Arg att aaa 1459 Ile Lys gct gcc tat cta Ala Ala Tyr Leu 395 tat ctt cag ctg Tyr Leu Gin Leu 410 380 gca Ala ggg Gly ctg cgt ggg gcc Leu Arg Gly Ala 400 gat acc tct att Asp Thr Ser Ile 385 aat ccc acc gca tat Asn Pro Thr Ala Tyr 405 ttg agt tcc tcg ccg Leu Ser Ser Ser Pro 420 415 ttc atc Phe Ile 425 ggc acc Gly Thr acc att gat tcg gca Thr Ile Asp Ser Ala 430 agg ccg cgt ggg cga Arg Pro Arg Gly Arg 445 ctg cgc agt aat cct Leu Arg Ser Asn Pro 460 ggg Gly tat gtg gaa tca aag ccc Tyr Val Giu Ser Lys Pro 435 440 aca gcg caa gaa gac caa gaa Thr Ala Gin Giu Asp Gin Giu 450 aaa gat cgt gca gaa aac ttg Lys Asp Arg Ala Giu Asn Leu atc att gct 1507 Ile Ile Ala 455 atg atc gtg 1555 Met Ile Val 470 acc aca gtt 1603 Thr Thr Val gag Giu gat ttg gtc Asp Leu Val 475 aaa aca tcc Lys Thr Ser cgc aac gac tta gcc Arg Asn Asp Leu Ala 480 aag ctt ttc gac gtc Lys Leu Phe Asp Val 495 acc gtc tct gca gag Thr Val Ser Ala Giu cgc ggc gct ttg ccc Arg Gly Ala Leu Pro 485 gaa acc tac gcc aca Giu Thr Tyr Ala Thr 500 ttg ggg cca cgc agt Leu Gly Pro Arg Ser 515 gtc cac 1651 Val H-is ccg att 1699 Pro Ile gcc cca 1747 Ala Pro 535 cct cgc 1795 Pro Arg 550 gca gtt 1843 Ala Val caa ctt Gin Leu 505 gag tgc Giu Cys 490 gtc Val agc Ser 510 gtg cgc gca gca Val Arg Ala Ala 525 520 aag Lys ttc Phe atc Ile ccc ggt ggt tcg atg act ggt Pro Gly Gly Ser Met Thr Gly 530 atc gat gag ctg gag gca gct Ile Asp Giu Leu Giu Ala Ala ctg cgc acc atg Leu Arg Thr Met 540 gag Glu ggt att tac tca Gly Ile Tyr Ser 555 gat ctc tcc atg Asp Leu Ser Met 570 ggt ggc ttg gga Gly Gly Leu Gly gtg atc aga act Val Ile Arg Thr 575 545 tat ttt Tyr Phe 560 ctc gtC Leu Val tcc ctc gac ggc Ser Leu Asp Gly 565 atc cag aac aat Ile Gin Asn Asn 580 WO 01/00843 WO 0100843PCT/IBO0100923 cac gtg gag tac gga gtg ggc ggt gca 1891 His Val Giu Tyr Gly Val Gly Gly Ala 585 590 gag gct gag tgg gag gaa atc cgc gtt 1939 Glu Ala Glu Trp Giu Glu Ile Arg Val 600 605 ttg ttt ggg gtt gaa ttc cca tgacgta 1983 Leu Phe Gly Val Glu Phe Pro 615 620 <210> 460 <211> 620 <212> PRT <213> Corynebacterium giutamicun <400> 460 Met Arg Vai Leu Ile Ile Asp Asn Tyr ctt ctt gct ctg tct gat ccg Leu Leu Ala Leu Ser Asp Pro 595 aaa tca cgg cct ctg ctg aat Lys Ser Arg Pro Leu Leu Asn 610 cct cgtgtgggac ggt 1 Ala Asn Pro Gly His Arg Leu Met Asp 145 Giy Ile Thr Tyr Gin Pro Ile Gly Val Ala 115 Ala Giy Gin Lys Lys 195 Val Giu Gly Giu Ile His 100 Gly Thr Leu Phe Asn 180 Thr Giu Ile His Arg Aia Giy Ile Arg Ile His 165 Phe Ile Giu Asp Ala Ala 70 Leu Giu Pro Leu Met 150 Pro Leu Pro Val Giu Gly Arg Ala Val Giu Pro 135 Ala Glu Asn Leu Thr Met Val1 Vai Tyr Ser Thr 120 Giu Leu Ser Leu Ser 200 Gly 25 Leu Ala Pro Gly Gin 105 Phe Ser Aia Ile Ala 185 Val1 Phe Pro Ala Phe Gly Val1 His Val1 Ala 140 Val Gin Tyr Ala Phe Val1 Ile Ile Cys Leu Gly 110 Tyr Ala Pro Giy Trp 190 Val Leu Pro Ser Ala Gly Pro Gly Ser Ser Trp 160 Gin Leu Glu WO 01/00843 WO 0100843PCT/EBOO/00923 Thr Gly 225 Thr Leu Val1 Ala Ala 305 Gin Ala Ser Thr Thr 385 Asn Leu Val1 Gin Arg 465 Arg Giu Leu Phe 210 Thr His Ala Gly His 290 Ile Asp Pro Lys Arg 370 Thr Pro Ser Giu Giu 450 Ala Gly Thr Gly Phe Ser Asn Ala Tyr 275 Thr Ala Giu Arg Asp 355 Gly Asp Thr Ser Ser 435 Asp Giu Ala Tyr Pro 515 Ala Tyr Val Asn 260 Val Ser Val Tip Ile 340 Giu Glu Val Ala Ser 420 Lys Gin Asn Leu Ala 500 Arg His Leu Gly 245 Ser Gly Ser Giu Phe 325 Pro Tyr Ser Ala Tyr 405 Pro Pro Giu Leu Pro 485 Thr S er Ser Gly 230 Giu Val1 Tyr Leu Ser 310 Giu Ala Leu Tyr Pro 390 Gly Glu Ile Ile Met 470 Thr Val Pro His Ala Asp Pro Leu 280 Asp Gin Thr Giy Lys 360 Ile Ala Tyr Phe Gly 440 Ala Val1 Val Gin Giu 520 Ala Ser Phe Gly 265 Lys Ala Val Ile His 345 Ile Cys Ala Leu Ile 425 Thr Glu Asp Lys Leu 505 Cys Phe Gly Phe 250 Gin Ala His Arg Lys 330 Leu Arg Leu Tyr Gin 410 Thr Arg Leu Leu Thr 490 Val Val1 Trp Pro 235 Thr Gly Giu Leu Leu 315 Lys Ala Arg Thr Leu 395 Leu Ile Pro Arg Val 475 Ser Ser Arg Leu 220 Leu Tip Phe Ala Ile 300 Leu Leu Leu Al a Thr 380 Ala Gly Asp Arg Ser 460 Arg Lys Thr Ala Asp Ala Leu Arg Gly 285 Phe Ala His Gin Gin 365 Lys Leu Asp Ser Giy 445 Asn Asn Leu Val1 Ala 525 Ala Thr Giu 255 Gly Arg Asp Gly Leu 335 Arg Leu Gin Gly Ser 415 Gly Thr Lys Leu Asp 495 Ala Pro Gin Lys 240 Asp Trp Ala Arg Giu 320 Val1 Asp Ile Gly Ala 400 Ile Tyr Ala Asp Ala 480 Val Glu Gly WO 01/00843 Gly Ser Me 530 Glu Leu Gi 545 Ala Pro 550 Lys Gly Phe Ser Leu Asp Gly Ala Val Asp Leu Ser Met 565 570 Val Ile Gin Asn Asn His Val Giu Tyr Gly Val 580 585 Ala Leu Ser Asp Pro Giu Ala Glu Trp Glu Giu .595 600 Arg Pro Leu Leu Asn Leu Phe Gly Val Glu Phe 610 615 <210> 461 <211> 747 <212> DNA <213> Corynebacterium giutamicum <22 0> <221> CDS <222> (101)..(724) <223> RXA00958 <400> 461 attctaatcc tcaatctgaa gccgatgaga cgttgcacaa Met 540 Gly Val Gly Ile Pro 620 PCTIIBOO/00923 Ile Ile Asp Leu Gly Tyr 560 Arg Thr Leu 575 Ala Leu Leu 590 Val Lys Ser ggcgtatgcc gtgttgaatg ccattgcgct tgctgctggt tccactttgg aggtcatccg atg aca cac gtt gtt Met Thr His Val Val ctc att gat Leu Ilie Asp gcc Ala gaa Giu cct Pro aca Thr ctc Leu ggC cac His tat Tyr gca Ala gcc Al a att Ile cac His aac gat Asp aag Lys gcc Ala gat Asp cc t Pro ggC dly atg tct Ser tgc Cys aac Asn gcg Al a tta Leu ggc Gly atc 1 ctg Leu aa t Asn tgc Cys gcg Ala ctc Leu tgt Cys ggt gat Asp gtg Val tca Ser atc Ilie tac Tyr cct Pro cag gcg Ala cca Pro cct Pro gag Glu cag Gin gtg Val 100 agc 115 163 211 259 307 355 403 451 WO 01/00843 Gly Thr Thr Asp Asn Met Ile Leu Thr 105 110 gtt ttt gca ggt ctt gcc act gat gtt Val Phe Ala Gly Leu Ala Thr Asp Val 120 125 cca ggc cgc aag gtt cca att ggc cgt Pro Gly Arg Lys Vai Pro Ile Gly Arg 135 140 gtt gcc cca gac ggt att gaa tca cta Val Ala Pro Asp Gly Ile Giu Ser Leu 150 155 ggt gat gtc atc atg gcg gca cgc acc Giy Asp Val Ile Met Ala Ala Arg Thr 170 ctg cag ttt cac cct gag tca gtg cta Leu Gin Phe His Pro Giu Ser Vai Leu 185 190 ttg tcc cgc tgt gtc gaa cag ctt ctc Leu Ser Arg Cys Vai Giu Gin Leu Leu.

200 205 tca <210> 462 <211> 208 <212> PRT <213> Corynebacteriun giutamicum <400> 462 Met Thr His Val Val Leu Ile Asp Asn 1 Leu Val Asp Aia Phe Ala Val Ala Gly 25 Asn Thr Val Pro Val Giu Thr Ilie Leu Cys Leu Ser Pro Gly Pro Giy Tyr Pro Ala Leu Ile Giu Arg Thr Leu Gly Gin 70 Leu Gly Tyr Gin Ala Leu Ilie Giu Tyr Cys Gly Pro Val His Gly Thr Thr Asp 100 .105 Gly Val Gin Ser Pro Val Phe Ala Gly 115 120 Asp His Pro Giu Ile Pro Gly Arg Lys Asp gag Giu tat Tyr ggt Gly acc Thr 175 agc Ser gcg Ala Ala cc t Pro cac His acc Thr 160 gat Asp cca Pro aac Asn PCTIIBOO/00923 Giy Val Gin Ser Pro 115 gat cat cca gaa atc 499 Asp His Pro Giu Ile 130 tca ctg ggc tgc gtg 547 Ser Leu Gly Cys Val 145 tgt tcc tcg gag att 595 Cys Ser Ser Giu Ile 165 gga aag gcc att ggc 643 Gly Lys Ala Ile Gly 180 acg ggt cct gtc att 691 Thr Gly Pro Val Ile 195 taataaaaaa aggatttgat 744 Val Val Asp Asn Gly Val Thr 110 Val1 Arg Asn Arg Ile Met Cys Pro Ala Pro His WO 01/008 130 Ser Leu 145 Cys Ser Gly Lys Thr Gly 43 43 PCTJIBOO/00923 135 140 Gly Cys Val Val Ala Pro Asp Gly Ile Giu Ser Leu Gly 150 155 Ser Giu Ile Gly Asp Val Ile Met Ala Ala Arg Thr Thr 165 170 175 Ala Ile Gly Leu Gin Phe His Pro Glu Ser Val L eu Ser 180 185 190 Pro Val Ile Leu Ser Arg Cys Val Giu Gin Leu Leu Ala 195 200 205 <210> 463 <211> 469 <212> DNA <213> Corynebacteriun glutaxnicum <220> <221> CDS <222> (469) <223> RXN03007 <400> 463 cagtttcacc ctgagtcagt gctaagccca acgggtcctg tcattttgtc ccgctgtgtc gaacagcttc tcgcgaacta ataaaaaaag gatttgattc atg act tct cca gca Met Thr Ser Pro Ala aca Thr gag Giu gac Asp cag Gin cgt Arg gg t Gly a tc Ile aaa Lys gca Al a cac His gc t Ala ttc Phe gac Asp gca Ala gtt ctc Val Leu att gag Ile Glu atc gca Ile Ala gat att Asp Ilie ccg att Pro Ile ggt gcc Gly Ala tcc ggt Ser Gly gcc Ala ttc Phe c tg Leu ggC Giy 9gC Gly ac c Thr gtg Val1 1 ccc Pro g tg Val1 cgt Arg ttc Phe gat Asp acc Thr cac act Thr ggt Gly act Thr ctc Leu tcc Ser ggc Gly 99c Lys Leu Val Lys His Gly Asn Arg Ser WO 01/00843 WO 0100843PCTIIBOOIOO923 105 -110 115 gtg agc tcc aag tcc ggc 469 Val Ser Ser Lys Ser Gly 120 <210> 464 <211> 123 <212> PRT <213> Corynebacteriun giutaiicun <400> 464 Met Thr Ser Pro Ala Thr Leu Lys Val Leu Asn Ala Tyr Leu Asp Asn 1 5 10 Pro Thr Pro Thr Leu Glu Glu Ala Ile Giu Val Phe Thr Pro Leu Thr 25 Val Gly Glu Tyr Asp Asp Val His Ile Ala Ala Leu Leu Ala Thr Ile 40 Arg Thr Arg Gly Glu Gln Phe Ala Asp Ile Ala Giy Ala Ala Lys Ala 55 Phe Leu Ala Ala Ala Arg Pro Phe Pro Ile Thr Gly Ala Gly Leu Leu 70 75 Asp Ser Ala Gly Thr Gly Gly Asp Gly Ala Asn Thr Ile Asn Ile Thr 90 Thr Gly Ala Ser Leu Ilie Ala Ala Ser Gly Gly Val Lys Leu Val Lys 100 105 110 His Gly Asn Arg Ser Val Ser Ser Lys Ser Gly 115 120 <210> 465 <211> 564 <212> DNA <213> Corynebacteriun glutamicum <22 0> <221> CDS <222> (101)..(541) <223> RXN02918 <400> 465 agattgtcgc ttttcccatt tctccgggtt ttctggaact ttttgggcgt atgctgggaa tgattctatt attgccaaat cagaaagcag gagagacccg atg agc gaa atc cta 115 Met Ser Glu Ile Leu 1 gaa acc tat tgg gca ccc cac ttt gga aaa acc gaa gaa gcc aca gca 163 Giu Thr Tyr Trp Ala Pro His Phe Gly Lys Thr Giu Glu Ala Thr Ala 15 ctc gtt tca tac ctg gca caa gct tcc ggc gat ccc att gag gtt cac 211 Leu Val Ser Tyr Leu Ala Gin Ala Ser Gly Asp Pro Ile Glu Val His WO 01/00843 WO 0100843PCT/IBOO/00923 30 acc ctg ttc ggg gat tta ggt tta gac gga ctc tct gga aac tac acc 259 Thr Leu Phe Gly Asp Leu Gly Leu Asp Gly Leu Ser Gly Asn Tyr Thr 45 gac act gag att gac ggc tac ggc gac gca ttc ctg ctg gtt gca gcg 307 Asp Thr Glu Ilie Asp Gly Tyr Gly Asp Ala Phe Leu Leu Val Ala Ala 60 cta tcc gtg ttg atg gct gaa aac aaa gca aca ggt ggc gtg aat ctg 355 Leu Ser Val Leu Met Ala Glu Asn Lys Ala Thr Gly Gly Val Asn Leu 75 80 ggt gag ctt ggg gga gct gat aaa tcg atc cgg ctg cat gtt gaa tcc 403 Gly Giu Leu Gly Gly Ala Asp Lys Ser Ile Arg Leu His Val Giu Ser 95 100 aag gag aac acc caa atc aac acc gca ttg aag tat ttt gcg ctc tcc 451 Lys Giu Asn Thr Gin Ilie Asn Thr Ala Leu Lys Tyr Phe Ala Leu Ser 105 110 115 cca gaa gac cac gca gca gca gat cgc ttc gat gag gat gac ctg tct 499 Pro Giu Asp His Ala Ala Ala Asp Arg Phe Asp Giu Asp Asp Leu Ser 120 125 130 gag ctt gcc aac ttg agt gaa gag ctg cgc gga cag ctg gac 541 Giu Leu Ala Asn Leu Ser Giu Giu Leu Arg Gly Gin Leu Asp 135 140 145 taattgtctc ccatttaagg agt 564 <210> 466 <211> 147 <212> PRT <213> Corynebacteriun giutamicum <400> 466 Met Ser Glu Ilie Leu Giu Thr Tyr Trp Ala Pro His Phe Gly Lys Thr 1 5 10 Giu Glu Ala Thr Ala Leu Val Ser Tyr Leu Ala Gin Ala Ser Gly Asp 25 Pro Ile Giu Vai His Thr Leu Phe Gly Asp Leu Gly Leu Asp Gly Leu 40 Ser Gly Asn Tyr Thr Asp Thr Giu Ile Asp Gly Tyr Gly Asp Ala Phe 55 Leu Leu Val Ala Ala Leu Ser Val Leu Met Ala Giu Asn Lys Ala Thr 70 75 Gly Gly Val Asn Leu Giy Giu Leu Giy Gly Ala Asp Lys Ser Ile Arg 90 Leu His Val Giu Ser Lys Glu Asn *Thr Gin Ile Asn Thr Ala Leu Lys 100 105 110 Tyr Phe Ala Leu Ser Pro Giu Asp His Ala Ala Ala Asp Arg Phe Asp WO 01/00843 WO 0100843PCT/EB00100923 125 Giu.Leu Arg Gly Glu Asp Asp Leu Ser Glu LeU Ala Asn Leu Ser Giu 130 135 140 Gin Leu Asp 145 <210> 467 <211> 735 <212> DNA <213> Corynebacteriun giutanicuxn <220> <221> <222> <223>

COS

(712) RXN0 1116 <400> 467 tgccaggaat ttacgtcaac cgcgtcgtcc acgttggacc gcaggaaacc ggaatcgaaa acaggacggt gtctaactaa tgacttggga tcataaccaa atg gca gcc cgc Met Ala Ala Arg 1 gcc cag gaa ctt Ala Gin Glu Leu gac ggc cag tac Asp Gly Gin Tyr aac ctc ggc atc Asn Leu Gly Ile ggc atg Gly Met cct aca ctt Pro Thr Leu cac tcc gaa Hius Ser Glu ccc ggc tac ctg Pro Gly Tyr Leu gag gga cta gag gtt atc ctt Glu Gly Leu Glu Val Ile Leu aac ggt gtg ctg Asn Gly Val Leu ggc Gly 45 gtt gga cct tac Val Gly Pro TIyr cca Pro act gaa gag Thr Glu Glu gaa ctt Glu Leu gat cct gag ctg Asp Pro Glu Leu aac gcc ggc aag Asn Ala Gly Lys acc atc acg gtt Thr Ile Thr Val gca Aila cct ggc gca tcc Pro Gly Ala Ser ttc tcc tct tct gat tct ttc gcc atg Phe Ser Ser Ser Asp Ser Phe Ala Met cgc tcc aag tct Arg Ser Lys Ser gac gtt gca gtc Asp Val Ala Val ggc gtt atg gaa Gly Vai Met Giu gtC tcc Val Ser 100 cag tac ggc Gin Tyr Gly ggt atg ggt Gly Met Gly 120 gac Asp 105 ctg gcc aac tgg Leu Ala Asn Trp atg Met 110 att ccc ggc aag ctg gtc aag Ile Pro Gly Lys Leu Val Lys 115 ggc gca atg gat Gly Ala Met Asp ctg Leu 125 gtg cac ggc gca Val His Gly Ala t cc Ser 130 aag atc atc Lys Ile Ile gcc atg acc gat cac atc Ala Met Thr Asp His Ilie 135 aag aag ggc gct Lys Lys Gly Ala ccg Pro 145 aag atc ctt aag Lys Ile Leu Lys WO 01/00843 WO 0100843PCTIiBOO/00923 tgt cgc ctc cca Cys Arg Leu Pro acc acc cac Thr Thr His atc gag tgc Ile Giu Cys gaa gcc gat Glu Ala Asp 200 ggc gcg aag tgc gtg gac atg att gtc Gly Ala Lys Cys Val Asp Met Ile Val 160 165 gtg gac cct gaa gaa ggc ctc acg ctc Val Asp Pro Glu Glu Gly Leu Thr Leu 175 180 acc gtt gag gaa ctc cgc gaa atc acc Thr Val Glu Glu Leu Arg Glu Ile Thr 190 195 taagcaaacg ctgcgcaatt aag 595 643 691 735 <210> 468 <211> 204 <212> PRT <213> Corynebacterium glutamicun <400> 468 Met Ala Ala Arg Val Ala Gin Glu Leu 1 Leu Leu Tyr Glu Ser Val1 Gly Ala Pro 145 Val1 Glu Leu Gly Glu Pro Thr Phe Met Lys Ser 130 Lys Asp Gly Arg Ile Gly Val Ilie Thr Glu Ile Thr Ala Met Glu Val 100 Leu Val 115 Lys Ilie Ile Leu Met Ilie Leu Thr 180 Glu Ile Glu Asp 10 Pro Gly Gly Val Giu Leu Ser Tyr 75 Val Asp Leu Aia Ala Met His Ile Pro Leu 155 Val Phe 170 Asp Gly Lys Val Gly Tyr Leu Ile Phe Val Asn Asp Thr 140 Thr Ser Val Ala Val1 Giu Gly Gly Ser Leu Ile His Gly Lys Pro 175 Glu Asn Giy Pro Lys Asp Gly Pro Giy Ala Cys 160 Giu Glu WO 01/00843 WO 0100843PCT/IBOO/00923 195 <210> 469 <211> 876 <212> DNA <213> Corynebacterium 200 glutaicurn <220> <221> <222> <223>

CDS

(101)..(853) RXN0 1115 <400> 469 ctcaccgcat gcagcgtgaa aacactcagt acggactggc caccatgtgc atcggtggcg gccagggtct tgcagctgtc tttgaaaagg agaactaaaa atg gct att ttg Met Ala Ile Leu 1 agc gtt tcc tac Ser Val Ser Tyr act tcc gac aac Thr Ser Asp Asn ttg gtg ttc att Leu Val Phe Ile ggc tcg Gly Ser ttg ggt tcc Leu Gly Ser aag gat ttc Lys Asp Phe acc gac atg tgg Thr Asp Met Trp cca cag ctg gat.

Pro Gin Leu Asp gcc ttg cat Ala Leu His cgc gtc atc gct Arg Val Ile Ala gtt gat cac cgc gga cat ggt ctg tct Val Asp His Arg Gly His Gly Leu Ser 45 act gtg gcg gat ctg gcg cag gat gtg Thr Val Ala Asp Leu Ala Gin Asp Val gaa ctc Glu Leu atc gaa ggc acc Ilie Glu Giy Thr gat acc ctc gat Asp Thr Leu Asp ctg ggt gtc gga Leu Gly Val Gly aac Asn ttc ggc gtc atc Phe Giy Val Ile cta tct ctc ggc Leu Ser Leu Gly gcg gtt gca caa Ala Val Ala Gin ttg gcg gcc acc Leu Ala Ala Thr tct gat Ser Asp 100 cgt gtc acc aag gca gca ttc atg tgt Arg Val Thr Lys Ala Ala Phe Met Cys 105 110 acc gct gca aaa Thr Ala Ala Lys ttc ggc gag Phe Gly Giu 115 aac ggc act Asn Gly Thr ccc cag ggc Pro Gln Gly 120 tgg cta gat cgc Trp Leu Asp Arg gca gcg tgc cgc Ala Ala Cys Arg ggt tct Gly Ser 135 ctg tcc gaa gct Leu Ser Glu Ala atc cag cgc tgg Ile Gln Arg Trp ttc Phe 145 tcc ccc act tgg Ser Pro Thr Trp gag aac aac cca Glu Asn Asn Pro tcc cgc gag cac Ser Arg Giu His gaa gcc atg gtt Glu Ala Met Val ggc acc cca tct gag ggt tac gcg ctg tgc tgc gag gcg ttg gca acc WO 01/00843 Gly Thr Pr tgg gat tt Trp Asp Ph atc gca gg Ile Ala Gi 2C att gcc ge Ile Ala As 215 gcc gcg ca Ala Ala Hi 230 cta gca ca Leu Ala GI Ser Glu 170 acc gat Thr Asp 185 gcc gat Ala Asp ggc gtt Giv Val Tyr Ala Leu Cys Cys Giu Ala 175 ctg gga gaa atc acc gtg cca Leu Gly Giu Ile Thr Val Pro 190 ccc tcc act cct cca gca acc Pro Ser Thr Pro Pro Ala Thr 205 210 ggc gag tcc cgc gca gag gtc Gly Glu Ser Arg Ala Giu Val 220 225 gtg gaa cgt cca aac gag gta Val Giu Arg Pro Asn Glu Val 240 taatgttgta ggcatgttca caa PCTIBOO/00923 Ala Thr 180 ctc. acc 691 Leu Thr cag atc 739 Gin Ile agc cca 787 Ser Pro gaa ctg 835 Giu Leu 245 cca Pro t tc Phe 250 <210> 470 <211> 251 <212> PRT <213> Corynebacterium glutanicun <400> 470 Met Aia Ile Leu His Ser Val Ser Tyr Gly Thr Ser Asp Asn Thr Leu Gly Leu Leu Asp Ile Ser 100 Giy Giy Thr Ser His Ser Val Giy Asp Glu Thr Trp 10 Thr Val Giy Asp Gly Aia Leu Glu Pro Trp Val Thr Gly Ala Met Ala 125 Ile Arg Leu Asp Val Vali Gin Cys 110 Aia Gin Glu Giu Aia Met Val Ala Gly Thr Pro Ser Giu Gly Tyr Ala Leu Cys Cys WO 01/00843 165 Thr Thr Ile Pro Leu 245 Trp Asp Phe Thr 185 Ile Ala Gly Ala 200 Ile Ala Asp Gly 215 Ala Ala His Val 230 Leu Ala Gin His Arg Leu Gly Giu 190 Asp Pro Ser Thr 205 Gly Gly Glu Ser 220 Thr Val Glu Arg 235 Ala PCTIIBOO/00923 175 Ile Thr Pro Pro Arg Ala Pro Asn 240 <210> 471 <211> 1284 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (101)..(1261) <223> RXS00116 <400> 471 cgcggcacgc acgctggggg caagcgtcga caagcacaaa ctttttgctt aattgaatcc tttgcgcacc aatcaatggg ggatcaaata tagtagctgc atg agt aat gac ttc Met Ser Asn Asp Phe gtc Val1 acc Thr cct Pro at t Ile ttg Leu tac Tyr at t Ile tc t Ser cga Arg gag Glu ggg Gly gca Ala cc t Pro gcg Ala aga Arg gag Giu cc t Pro aat Asn gc t Ala gag Glu ttg Leu ccc Pro gcg Ala cgt Arg cag Gin cgt Arg gtg Val ggt Gly ttt Phe ggt Gly c9g Arg 45 tat Tyr gat Asp ttg Leu t tg Leu gaa Giu atc Ile tta Leu gcg Ala t tg Leu acg Thr gag Glu 1 att Ile ctt Leu atc Ile cgt Arg agg Arg ggg Gly ggg Gly ttt Phe ggt Gly gcg Ala ggg Gly ttt Phe gcc Ala gat Asp gca Ala cag Gin tcg Ser ga t Asp gat Asp act Thr gaa Glu acc Thr ggc Gly gag Glu gc t Ala ctg Leu gag Glu 100 gtg Val1 115 163 211 259 307 355 403 451 WO 01/00843 WO 0100843PCTlIBOO/00923 gtt ttg gaa Val Leu Giu 120 ccg tat tac gat Pro Tyr Tyr Asp tat gcg gcg gct Tyr Ala Ala Ala att Ile 130 gcg ttg gcg Ala Leu Ala ggg gcg Gly Ala 135 acg cgg gtg gcg Thr Arg Val Ala cct ttg cag gag Pro Leu Gin Giu g tg Val1 145 gag aac tcg tgg Glu Asn Ser Trp gat Asp 150 gtg gat gtc gat Val Asp Val Asp ttg cat gcg gcg Leu His Ala Ala act aag aag acg Thr Lys Lys Thr atg att atc gtt Met Ile Ile Val tcg ccg cat aat Ser Pro His Asn acg ggt tcg gtg Thr Gly Ser Val ttt tct Phe Ser 180 aag aag gcg Lys Lys Ala ttg gtg ttg Leu Val Leu 200 aag cag ttg gcg Lys Gin Leu Ala gtt gct cgt gcg Val Ala Arg Ala tat gac ttg Tyr Asp Leu 195 gat gat cag Asp Asp Gin tca gat gag gtg Ser Asp Giu Val gag cat ctt gtt Giu His Leu Val aag cat Lys His 215 gtg agt gtc gcg Val Ser Val Ala ctg ccc ggt atg Leu Pro Gly Met tgg Trp 225 gat cgc acg gtg Asp Arg Thr Val acg Thr 230 gtg tcg tcg gcg Val Ser Ser Ala aaa acg ttc aat Lys Thr Phe' Asn 4tg Val 240 act ggt tgg aag Thr Gly Trp Lys ggg tgg gcg ttg Gly Trp Ala Leu ccg gag ccg ttg Pro Giu Pro Leu gag gcg gtg ttg Glu Ala Val Leu aag gcg Lys Ala 260 aag cag ttt Lys Gin Phe gcg cat gcg Ala His Ala 280 tct tat gtg ggg Ser Tyr Val Gly aca cct ttt cag Thr Pro Phe Gin ccg gct gtg Pro Ala Val 275 atg tct aag Met Ser Lys att gaa cat gag Ile Giu His Glu aag tgg gtg tca Lys Trp Val Ser aag Lys 290 ggg ctt gag ctc aag cgg gat att ttg cgt act gcg tta gat aag gcg 1027 Gly Leu 295 ggg ctg 1075 Gly Leu 310 att ggg 1123 Ile Gly Glu Leu Lys Arg Ile Leu Arg Thr Ala 305 Leu Asp Lys Ala aag act cat gac agt atg ggc acg tat ttc atc gtt gcg gat Lys Thr His Asp 315 Ser Met Gly Thr Tyr 320 Phe Ile Val Ala Asp 325 gat cgt gat ggt gcg gag ttc tgt ttt gag ttg att gag aag Asp Arg Asp 330 Gly Ala Glu Phe Phe Glu Leu Ile Giu Lys 340 WO 01/00843 gtt ggg gtg gcg gcg att ccg gtg cag gcg ttt 1171 Val Gly Val Ala Ala Ile Pro Vai Gin Ala Phe 345 350 aag tgg tcg tcg aag gtt cgt ttt gcg ttt tgc 1219 Lys Trp Ser Ser Lys Val Arg Phe Ala Phe Cys 360 365.

ctc cgc gaa gct gcg gag cgt ctc aag ggg att 1261 Leu Arg Giu Aia Ala Giu Arg Leu Lys Giy Ile 375 380 tagtttgaac aggttgttgg ggg 1284 <210> 472 <211> 387 <212> PRT <213> Corynebacterium giutamicux PCTIBOO/00923 gat cat ccg aag Asp His Pro Lys 355 aaa gaa gag acg Lys Giu Giu Thr 370 aaa cta Lys Leu <400> 472 Met Ser Asn Asp Phe Val Val Ser Arg Thr Gly Glu Ala Leu Glu 100 Val Leu Ser Thr Phe 180 Met Phe Gin Ser Glu Ala Ile Ala Trp Arg 165 Ser Leu 10 Val Gly Asn Val Ser 90 Va1 Tyr Val Asp Asn 170 Lys Arg Pro Glu Ala Pro Arg Asn Gin Ala Arg Glu Val Leu Gly Tyr Asp Ala Vai 140 Lys Leu 155 Ser Pro Gin Leu Phe Gly Arg Tyr Asp Leu Leu Ala 125 Pro His His Ala Gly Ala Met Ser His Ile Val 110 Tyr Leu Ala Asn Gly 190 Glu Ile Leu Ala Leu Thr Glu Ala Gin Ala Pro 175 Val Thr Asn Glu Gly Glu Val Pro Ala Glu Val 160 Thr Ala WO 01/00843 PCTIBOO/00923 Arg Ala Tyr Asp Leu Leu Val Leu Ser Asp Giu Val Tyr Giu His Leu 195 200 205 Val Phe Asp Asp Gin Lys His Val Ser Val Ala Lys Leu Pro Gly Met 210 215 220 Trp Asp Arg Thr Val Thr Val Ser Ser Ala Ala Lys Thr Phe Asn Val 225 230 235 240 Thr Gly Trp Lys Thr Gly Trp Ala Leu Ala Pro Giu Pro Leu Leu Glu 245 250 255 Ala Val Leu Lys Ala Lys Gin Phe Met Ser Tyr Val Gly Ala Thr Pro 260 265 270 Phe Gin Pro Ala Val Ala His Ala Ile Giu His Giu Gin Lys Trp Vai 275 280 285 Ser Lys Met Ser Lys Giy Leu Giu Leu Lys Arg Asp Ile Leu Arg Thr 290 295 300 Ala Leu Asp Lys Ala Gly Leu Lys Thr His Asp Ser Met Gly Thr Tyr 305 310 315 320 Phe Ile Val Ala Asp Ile Gly Asp Arg Asp Gly Ala Giu Phe Cys Phe 325 330 335 Giu Leu Ilie Glu Lys Val Gly Val Ala Ala Ile Pro Val Gin Ala Phe 340 345 350 Val Asp Hius Pro Lys Lys Trp Ser Ser Lys Val Arg Phe Ala Phe Cys 355 360 365 Lys Lys Giu Giu Thr Leu Arg Giu Ala Ala Glu Arg Leu Lys Gly Ile 370 375 380 Lys Lys Leu 385 <210> 473 <211> 607 <212> DNA <213> Corynebacteriui giutanicun <220> <221> CDS <222> (101)..(607) <223> FR.XA00116 <400> 473 tttgcgcacc aatcaatggg ggatcaaata tagtagctgc atgagtaatg acttcgtcgt ttctaggctt agaccctttg gtgaaacgat ttttgcaacc atg acc cag cga gct 115 Met Thr Gin Arg Ala 1 gtt gag gcg ggt gca atc aat ctt ggt cag ggc ttt cct gat gag gat 163 Val Giu Ala Gly Ala Ile Asn Leu Gly Gin Gly Phe Pro Asp Giu Asp 15 WO 01/00843 ggt cct cgt cgg atg tta gag atc gcg tcg PCT/IBOO/00923 Giy Pro Arg Met Leu Giu Ile Ala Ser aat Asn gtg Val1 tcg Ser gtg Val1 tat Tyr gtg Val1 ga t Asp aa t ASn 150 aag Lys gcg Ala t tg Leu acg Thr 75 gag Glu gcg Al a cag Gin gcg Ala ccg Pro 155 ggg Gly gag Giu gtg Val1 cct Pro gcg Ala gag Giu gtg Val 140 acg Thr gag cag att ctc ggg Giu Gin Ile Leu Gly gct tcg ttg agg gca Ala Ser Leu Arg Ala ctg gag tac aac cct Leu Giu Tyr Asn Pro gag gcg att acg gcg Giu Ala Ile Thr Ala gtg atc gtt ttg gaa Val Ile Vai Leu Giu 100 ttg gcg ggg gcg acg Leu Aia Gly Aia Thr 115 tcg tgg gat gtg gat Ser Trp Asp Val Asp 130 acg cgg atg att atc Thr Arg Met Ile Ile 145 ttt tct aag aag gcg Phe Ser Lys Lys Ala 160 cgg Arg gtc Val1 gtt Val1 ttg Leu 165 <210> 474 <211> 169 <212> PRT <213> Corynebacterium giutamicumr <400> 474 Met Thr Gin Arg Ala Val Giu Ala Gly 1 5 Phe Pro Asp Giu Asp Gly Pro Arg Arg 25 Gin Ilie Leu Gly Gly Asn Asn Gin Tyr 40 Ser Leu Arg Ala Ala Val Ala Arg Asp 55 Giu Tyr Asn Pro Asp Ser Glu Val Leu 70 Ile Asn Leu Giy Gin Leu Giu Ile Aia Ser Ala Giy Arg Gly Asp Leu Giu Arg Phe Asp Thr Val Gly Ala Thr WO 01/00843 WO 0100843PCTIBOO/00923 Ala Ile Ala Trp Arg 145 Ser Thr Pro Arg Val1 Val1 Leu 165 Pro Gly Asp Giu Ala Ala Ile Ala 110 Giu Val Giu Asn 125 Val Thr Lys Lys 140 Thr Gly Ser Val Val Leu Ser Thr Phe 160 <210> 475 <211> 843 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(820) <223> RXS00391 <400> 475 atcttgtaga tcccaccgca attctgggag atctcgagga tttcctcccc atccctgtac aagataaaac ccgtgcacag caa Gin act Thr aat Asn ggc Giy gtg Val ctc Leu gtt Val ggt Gly gtg Vai tgg Trp ctt Leu aca Thr ggc Gly tct Ser agt Ser ttg Leu cga Arg ggt Gly cgcaatctct ggaagaaaac ttg ctg cgc gat tct Leu Leu Arg Asp Ser 1 gct ttg gtg atg gcc Ala Leu Vai Met Ala cag ctc act ccg ttg Gin Leu Thr Pro Leu ttt tta ggt ggc gaa Phe Leu Gly Gly Giu att gca ggc atg cag Ile Ala Gly Met Gin cca cta gct att gat Pro Leu Ala Ile Asp ggc gcc gcg gca gaa Gly Ala Ala Ala Glu 100 WO 01/00843 ctg aaa aat Leu Lys Asn tta ctt aaa Leu Leu Lys 120 ttt gat gtc Phe Asp Val 135 att tct gcg Ile Ser Ala 150 tca gag act Ser Giu Thr gcg aaa gtc Ala Lys Val att gcg cag Ile Ala Gin 200 gag ggt tgg Giu Gly Trp 215 ggc Gly atg Met ctt Leu cag Gin ggt Giy 170 att Ile tac Tyr acc Thr tcc ttg act cca Ser Leu Thr Pro 115 att gaa gcc ctg Ile Giu Ala Leu 130 ttg tct aag cag Leu Ser Lys Gin 145 gtc acc acc tac Val Thr Thr Tyr 160 ggc aag ccc att Giy Lys Pro Ile gag ttg ggt ggc Giu Leu Gly Giy 195 cat ccg gat ttc His Pro Asp Phe 210 gaa ctc cac gac Giu Leu His Asp 225 PCTIBOOIOO923 atg cag 451 Met Gin aaa ctt 499 Lys Leu gcc cga 547 Ala Arg ggc tcc 595 Gly Ser 165 ccc ggc 643 Pro Gly 180 ccg atg 691 Pro Met gcc aac 739 Ala Asn ggg att 787 Giy Ile ctc acc gtg act ggt cgc gtg gat Leu Thr Vai Thr Gly Arg Vai Asp 230 235 tgc <210> 476 <211> 240 <212> PRT <213> Corynebacteriun giutanicum <400> 476 Leu Leu Arg Asp Ser Gin Arg Val 1 Ala Leu Vai Met Ala Thr Ser Gly Gin Leu Thr Pro Leu Asn Leu Val 40 Phe Leu Giy Gly Giu Giy Gin Trp 55 Ile Ala Gly Met Gin Val Leu Leu Pro Leu Ala Ile Asp Leu Ser Thr acc cgt I'hr Arg tgattccggt ggattgaagt 840 3iy Ser Ser .jeu krg 3iY 690 Ilie Thr Asp Met Ile Ile Asp Pro Ala Pro Ala Asp WO 01/00843 Gly Leu Giu Ser 145 Thr Lys Leu Pro Leu 225 Ala Thr Al a 130 Lys Thr Pro Gly Asp 210 His Ala Ala 100 Pro Met 115 Leu Lys Gin Ala Tyr Gly Ilie Pro 180 Gly Pro 195 Phe Ala Asp Gly.

Giu Gin Leu Arg Ser 165 Gly Met Asn Ile Leu Leu Phe Ile 150 Ser Ala Ile Giu Leu 230 Lys Leu Asp 135 Ser Glu Lys Ala Gly 215 Thr Asn Lys 120 Val1 Ala Thr Vali Gin 200 Trp, Val Tyr 110 Gin Ala Asn Tyr Arg 190 Pro Ser Thr PCTILBOOIOO923 Thr Ser Gly Ile Ala Leu Ile Val 160 Asp Gly 175 Ile Glu Glu His Gly Glu Arg His 240 <210> 477 <211> 1017 <212> DNA <213> Corynebacteriun glutamicun <22 0> <221> CDS <222> (101)..(994) <223> RXS00393 <400> 477 tctattcatt tcacaatagc gtttcacact cccccatagc aattgcgcga tcgagtatgt gatggggaaa gatagaggtt ccc cag ccg Pro Gin Pro ccg cgt acc Pro Arg Thr gtc gcc gct Val Ala Ala aat tct gta act ttg Asn Ser Vai Thr Leu tgg gca aat gcg ttc Trp, Ala Asn Ala Phe ttt cat gat ggt ttt Phe His Asp Gly Phe 45 gat tgg Asp Trp i5 cct gtc Pro Vai tgg tgg Trp Trp, ctgccgaacg tatttcaagc atg tct cac acg gaa Met Ser His Thr Giu 1 att caa ggc gca cgc Ile Gin Gly Ala Arg att gcc ggt tca ggt Ile Ala Gly Ser Gly aag gcc ttg ctg gcg Lys Aia Leu Leu Ala WO 01/00843 WO 0100843PCTJ1B00/00923 ctt gtc Leu Val gtg gcg tgg gct Val Ala Trp, Ala atc atc ggt gtg Ile Ile Gly Val tac gcc aat gat Tyr Ala Asri Asp tac Tyr cga Arg tct gat ggc att Ser Asp Gly Ilie ctc act ggt tct Leu Thr Gly Ser ggc acc gat gaa Gly Thr Asp Glu cgc acc ggt cct Arg Thr Gly Pro ggg ttg gct gag Gly Leu Ala Glu aag aaa gtg aaa Lys Lys Val Lys gct gcg Ala Ala 100 gcg ttt att Ala Phe Ilie ctg ttg agc Leu Leu Ser 120 ttc ggt atc gca Phe Gly Ilie Ala ggt Gly 110 gtc gcc ggc acc Val Ala Gly Thr gcg ctg agc Ala Leu Ser 115 tgt gtg ctg Cys Val Leu gcg tgg tgg ctg Ala Trp Trp Leu atc Ile 125 ctc atc ggc atc Leu Ilie Gly Ilie ggc gcg Gly Ala 135 tgg ttc tac acc Trp Phe Tyr Thr ggt aaa aat cct Gly Lys Asn Pro tat Tyr 145 ggt tac cgc ggg Gly Tyr Arg Gly ggc gag att gct gtg ttc atc ttc ttc Gly Glu Ile Ala Val Phe Ile Phe Phe 155 9gC Gly 160 ctc gtc gcg gtc Leu Val Ala Val gga acg cag ttc Gly Thr Gin Phe caa acc ggt tcc Gin Thr Gly Ser agc tgg gcc ggt Ser Trp Ala Gly ttg gcc Leu Ala 180 gcc gca gtt Ala Ala Val aat att cgc Asn Ile Arg 200 gtg ggg tcg atg Val Gly Ser Met gct ggc gtg aac Ala Gly Val Asn ttg gcc aac Leu Ala Asn 195 att acc ctc Ile Thr Leu gat att cca acc gat Asp Ilie Pro Thr Asp 205 agc aag acc gga Ser Lys Thr Gly aaa Lys 210 gcg gtc Ala Val 215 cgc ctg ggc gat Arg Leu Gly Asp ggt gct cgt aag Gly Ala Arg Lys ctg Leu 225 ttc ctc gcg ctg Phe Leu Ala Leu att Ile 230 tcc acg ccg ttc Ser Thr Pro Phe atg tcc atc tgc Met Ser Ile Cys c tg Leu 240 gcg ttt gtc gcc Ala Phe Val Ala 787 835 883 cca gcg ctg atc Pro Ala Leu Ilie atc atc gtt ttc Ile Ile Val Phe ctg gca ctg aaa Leu Ala Leu Lys gcc gca Ala Ala 260 ggg ccg atc Gly Pro Ile ggc tca aca Gly Ser Thr 280 aac aac gcc acc Asn Asn Ala Thr aag gat ctc atc Lys Asp Leu Ile ccc gtc atc Pro Val Ilie 275 acg ggc ctg Thr Gly Leu ggg cgc gcc atg Gly Arg Ala Met ttg tgg gcc gtg Leu Trp Ala Val ctc Leu 290 WO 01/00843 PCTIIBOO/00923 gca tta gcg ttt agc taaaacgctt ttcgacgctc CCC 1017 Ala Leu Ala Phe Ser 295 <210> 478 <211> 298 <212> PRT <213> Corynebacterium giutamicum <400> 478 Met Ser His Thr Glu Pro Gin Pro Asn Ser Val Thr Leu Ser Asp Tip 1 5 10 Ilie Gin Gly Ala Arg Pro Arg Thr Tip Ala Asn Ala Phe Ala Pro Val 25 Ilie Ala Gly Ser Gly Val Ala Ala Phe His Asp Gly Phe Val Trp Tip 40 Lys Ala Leu Leu Ala Leu Val Val Ala Trp Ala Leu Ile Ile Gly Val 55 Asn Tyr Ala Asn Asp Tyr Ser Asp Gly Ilie Arg Gly Thr Asp Glu Asp 70 75 Arg Thr Gly Pro Leu Arg Leu Thr Gly Ser Gly Leu Ala Glu Pro Lys 90 Lys Val Lys Ala Ala Ala Phe Ilie Ser Phe Gly Ile Ala Gly Val Ala 100 105 110 Gly Thr Ala Leu Ser Leu Leu Ser Ala Trp Tip Leu Ile Leu Ile Gly 115 120 125 Ilie Leu Cys Val Leu Gly Ala Tip Phe Tyr Thr Gly Gly Lys Asn Pro 130 135 140 Tyr Gly Tyr Arg Gly Leu Gly Giu Ile Ala Val Phe Ile Phe Phe Gly 145 150 155 160 Leu Val Ala Val Met Gly Thr Gin Phe Thr Gin Thr Gly Ser Val Ser 165 170 175 Tip Ala Gly Leu Ala Ala Ala Val Gly Val Gly Ser Met Ser Ala Gly 180 185 190 Val Asn Leu Ala Asn Asn Ile Arg Asp Ile Pro Thr Asp Ser Lys Thr 195 200 205 Gly Lys Ilie Thr Leu Ala Val Arg Leu Gly Asp Ala Gly Ala Arg Lys 210 215 220 Leu Phe Leu Ala Leu Ile Ser Thr Pro Phe Ile Met Ser Ile Cys Leu 225 230 235 240 Ala Phe Val Ala Tip Pro Ala Leu Ile Ala Ile Ile Val Phe Pro Leu 245 250 255 Ala Leu Lys Ala Ala Gly Pro Ile Arg Asn Asn Ala Thr Gly Lys Asp WO 01/00843 WO 0100843PCTI/iBOO/00923 270 Arg Ala Met Ala Leu Trp, Ala 285 Leu Ilie Pro Val Ilie Gly Ser Thr Gly 275 280 Val Leu Thr Gly Leu Ala Leu Ala Phe Ser 290 295 <210> 479 <211> 1005 <212> DNA <213> Coryriebacteriun giutanicum <220> <221> <222> <223>

CDS

(101)..(982) FRXA00393 <400> 479 tctattcatt tcacaatagc gtttcacact cccccatagc ctgccgaacg tatttcaagc aattgcgcga tcgagtatgt gatggggaaa gatagaggtt atg tct cac acg Met Ser His Thr 1 115 ccc cag ccg aat Pro Gin Pro Asn tct Ser gta act ttg tcc Val Thr Leu Ser tgg att caa ggc Trp Ile Gin Gly gca cgc Ala Arg ccg cgt acc Pro Arg Thr gtc gcc gct Vai Aia Ala gca aat gcg ttc Ala Asn Ala Phe cct gtc att gcc Pro Val Ile Ala ggt tca ggt Gly Ser Gly ttg ctg gcg Leu Leu Ala ttt cat gat ggt Phe His Asp Gly gtg tgg tgg aag Val Trp Trp Lys ctt gtc Leu Val gtg gcg tgg gct Val Ala Trp Ala atc atc ggt gtg Ile Ilie Gly Val tac gcc aat gat Tyr Ala Asn ASP 211 259 307 355 403 tac Tyr tct gat ggc att Ser Asp Gly Ile ggc acc gat gaa Gly Thr Asp Giu cgc acc ggt cct Arg Thr Giy Pro cga ctc act ggt Arg Leu Thr Gly tct Ser ggg ttg gct gag ccg aag aaa gtg aaa Gly Leu Ala Giu Pro Lys Lys Val Lys 95 gct gcg Ala Ala 100 gcg ttt att Ala Phe Ile ctg ttg agc Leu Leu Ser 120 ggC gcg tgg Gly Ala Trp 135 ttc ggt atc gca Phe Gly Ile Ala gtc gcc ggc acc Val Ala Giy Thr gcg ctg agc Ala Leu Ser 115 tgt gtg ctg Cys Val Leu 451 gcg tgg tgg ctg atc ctc atc ggc atc Ala Trp Trp Leu Ile Leu Ilie Gly Ile ttc tac acc Phe Tyr Thr ggc Gly 140 ggt aaa aat cct Gly Lys Asn Pro tat Tyr 145 ggt tac cgc ggg Gly Tyr Arg Gly WO 01/00843 WO 0100843PCT/iBOO/00923 ctc Leu 150 gga Gly gcc Ala aat Asn gcg Ala att Ile 230 cca Pro ggg Gly gct Ala ggc gag att gct Gly Giu Ile Ala acg cag ttc acc Thr Gin Phe Thr 170 gca gtt ggc gtg Ala Val Gly Val 185 att cgc gat att Ile Arg Asp Ilie 200 gtC cgC ctg ggc Val Arg Leu Gly 215 tcc acg ccg ttc Ser Thr Pro Phe gcg ctg atc gcg Ala Leu Ile Ala 250 ccg atc cgc aac Pro Ilie Arg Asn 265 caa cag ggc gcg Gin Gin Gly Ala gtg Val 155 caa Gin ggg Giy cca Pro gat Asp atc Ile 235 a tc Ile aac Asn cca Pro gtc Val gcc Ala aac Asn aa a Lys 210 t tC Phe ttt Phe ctg Leu atc Ile tca Ser 290 280 cat tagcgtttag ctaaaacgct ttt 1005 His <210> 480 <211> 294 <212> PRT <213> Corynebacteriun glutamicun <400> 480 Met Ser His Thr Glu Pro Gin Pro Asn 1 5 Ilie Gin Gly Ala Arg Pro Arg Thr Trp 25 Ilie Ala Gly Ser Gly Val Ala Ala Phe 40 Lys Ala Leu Leu Ala Leu Val Val Ala 55 Ser Val Thr Leu Ser Asp Trp 10 Ala Asn Ala Phe Ala Pro Val His Asp Gly Phe Val Trp, Trp Trp Ala Leu Ile Ile Gly Val WO 01/00843 WO 0100843PCTIBOOIOO923 Asn Tyr Ala Asn Asp Tyr Ser Asp Gly Ilie Arg Gly Thr Asp Giu 70 75 Arg Thr Lys Val Gly Thr Ile Leu 130 Tyr Gly 145 Leu Val Trp Ala Val Asn Gly Lys 210 Leu Phe 225 Ala Phe Ala Leu Leu Ilie Cys Ser 290 Pro Ala 100 Leu Val1 Arg Val1 Leu 180 Ala Thr Ala Ala Ala 260 Ser Ala Leu Ala Ser Leu Gly Met 165 Al a Asn Leu Leu Trp 245 Al a Ser Trp Arg Ala Leu Gly Leu 150 Gly Ala Asn Ala Ile 230 Pro Gly Ala His Leu Phe Leu Ala 135 Gly Thr Ala Ile Val 215 Ser Ala Pro Gin Thr Ile Ser 120 Trp Giu Gin Val1 Arg 200 Arg Thr Leu Ile Gin 280 Gly Gly Trp Thr Val1 155 Gin Gly Pro Asp Ile 235 Ile Asn Pro Leu Ile Leu Gly 140 Phe Thr Ser Thr Ala 220 Met Ile Ala Trp Ala Ala Ile 125 Gly Ilie Gly Met Asp 205 Gly Ser Val1 Thr Arg 285 Glu Gly 110 Leu Lys Phe S er Ser 190 Ser Ala Ile Phe Gly 270 Cys Pro Val1 Ile Asn Phe Val1 175 Ala Lys Arg Cys Pro 255 Lys Gly Asp Lys Ala Gly Pro Gly 160 Ser Gly Thr Lys Leu 240 Leu Asp Pro <210> 481 <211> 987 <212> DNA <213> Corynebacteriun glutarnicum <220> <221> CDS <222> (101)..(964) <223> RXS00446 <400> 481 tgctacgaag ttatctagta atgaagttag tttttcccct ctcccggcag cagttgatgc ggtgacggag gctacttggg gggctaatcg gtacccggat atg ggt gcg gtt gag 115 Met Gly Ala Val Glu WO 01/00843 ctc cgt gag gct ctt gca gag cat tta Leu Arg Glu Ala Leu Ala Glu His Leu acg Thr acg Thr gct Ala ccg Pro atc Ile tcg Ser gtt Val aac Asn gac Asp 150 ggc Gly atg Met gca Al a gag Glu gta Val tgc Cys tat Tyr c tg Leu act Thr ggc Gly cca Pro cgc Arg 135 aac Asn t tg Leu aat Asn gcg Ala gaa Glu 215 ggt Gly gc t Ala cca Pro act Thr ga t Asp acc Thr aac Asn 120 gcg Ala gtg Val1 cgt Arg aag Lys ctt Leu 200 acc Thr tgC Cys cag Gin att Ile gc t Ala aag Lys acc Thr 105 gat Asp gac Asp at t Ile gtt Val gtg Val1 185 gcg Ala gtc Val1 ggc Gly ggc Gly ttC Phe gat Asp acc Thr a tc Ile gtc Val gac Asp ggt Gly ggt Gly 170 gc t Ala agt Ser gaa Giu tcg Ser gat Asp gcg Ala cag Gin 75 cgc Arg ac c Thr gt t Val ac c Thr t tg Leu 155 tac Tyr att Ile ttg Leu aag Lys tct Ser gag Giu cag Gin 60 aat Asn c tc Leu cag Gin gtt Val cca Pro 140 cgc Arg gcc Ala cc t Pro aat Asn cgt Arg 220 gcg Ala gtC Val1 45 gtc Val cat His att Ile gcg Ala ggg Gly 125 gtt Val1 acg Thr ttC Phe t tC Phe tct Ser 205 gat Asp ctg Leu 30 att Ile gcg Al a gat Asp t tC Phe cag Gin 110 ctg Leu gcc Al a t tc Phe gga Gly gcg Al a 190 gcc Ala gct Al a tgg Trp gag gtt gag Giu Val Glu 15 tgt caa cag Cys Gin Gin ttt cca tgg Phe Pro Trp ggc gcc act Gly Ala Thr ctt gat gcg Leu Asp Ala 80 atc tgc aac Ile Cys Asn 95 ttt gat aat Phe Asp Asn gat gag gct Asp Giu Ala act gag gaa Thr Glu Glu 145 tcc aag gcg Ser Lys Ala 160 aac gca gag Asn Ala Giu 175 gtg aat tca Val Asn Ser gat gag ttg Asp Glu Leu gtg gtg tca Val Val Ser 225 ctg ccg ggc Leu Pro Gly 240 ttt Phe ctg Leu cgc Arg cct Pro atg Met ccc Pro ttC Phe tat Tyr 130 atc Ile tat Tyr atc Ile gca Ala atg Met 210 gcg Ala gag Glu gac Asp gtt Val1 agc Ser gt t Val1 gca Ala aac Asn atg Met 115 ttt Phe cac His ggc Gly atc Ile gct Ala 195 gaa Giu ctt Leu ggc Gly PCTIBOO/00923 cag gtc 163 Gin Val cag gca 211 Gin Ala ttt gag 259 Phe Glu gcc att 307 Ala Ile gcc gcg 355 Ala Ala aat cct 403 ASn Pro 100 gaa aag 451 Giu Lys gag ttc 499 Glu Phe cgc cac 547 Arg His ctg gcg 595 Leu Ala 165 gca gcg 643 Ala Ala 180 cag gcg 691 Gin Ala cgg gtg 739 Arg Val ggt gct 787 Gly Ala gcc gct 835 Ala Ala 245 gcg ccg acg cag gcc Ala Pro Thr Gin Ala 230 aat ttc gtc Asn Phe Val 235 WO 01/00843 PCT/IB00/00923 gag ttg gcg gct aaa ttg gcc gag cac ggc ate gtg att cgc gcg ttc 883 Glu Leu Ala Ala Lys Leu Ala Glu His Gly Ile Val Ile Arg Ala Phe 250 255 260 ccc gag ggt gcg cgc att tcg gtg acc aac gcc gag gaa act gac aag 931 Pro Glu Gly Ala Arg Ile Ser Val Thr Asn Ala Glu Glu Thr Asp Lys 265 270 275 ctg ctg cgc gcg tgg gag gcc ate aat gct ggg tagtctttgg cgttttgcgg 984 Leu Leu Arg Ala Trp Glu Ala Ile Asn Ala Gly 280 285 tgc 987 <210> 482 <211> 288 <212> PRT <213> Corynebacterium glutamicum <400> 482 Met Gly Ala Val Glu Leu Arg Glu Ala Leu Ala Glu His Leu Glu Val 1 5 10 Glu Phe Asp Gin Val Thr Val Gly Cys Gly Ser Ser Ala Leu Cys Gin 25 Gin Leu Val Gin Ala Thr Cys Ala Gin Gly Asp Glu Val Ile Phe Pro 40 Trp Arg Ser Phe Glu Ala Tyr Pro Ile Phe Ala Gin Val Ala Gly Ala 55 Thr Pro Val Ala Ile Pro Leu Thr Ala Asp Gin Asn His Asp Leu Asp 70 75 Ala Met Ala Ala Ala Ile Thr Asp Lys Thr Arg Leu Ile Phe Ile Cys 90 Asn Pro Asn Asn Pro Ser Gly Thr Thr Ile Thr Gin Ala Gin Phe Asp 100 105 110 Asn Phe Met Glu Lys Val Pro Asn Asp Val Val Val Gly Leu Asp Glu 115 120 125 Ala Tyr Phe Glu Phe Asn Arg Ala Asp Asp Thr Pro Val Ala Thr Glu 130 135 140 Glu Ile His Arg His Asp Asn Val Ile Gly Leu Arg Thr Phe Ser Lys 145 150 155 160 Ala Tyr Gly Leu Ala Gly Leu Arg Val Gly Tyr Ala Phe Gly Asn Ala 165 170 175 Glu Ile Ile Ala Ala Met Asn Lys Val Ala Ile Pro Phe Ala Val Asn 180 185 190 Ser Ala Ala Gin Ala Ala Ala Leu Ala Ser Leu Asn Ser Ala Asp Glu 195 200 205 WO 01/00843 WO 0100843PCTIBOO/00923 Leu Met 210 Giu Arg Val Giu Thr Val Giu Lys Arg 220 Asp Ala Val Val Ala Leu Gly Ala Pro Thr Gin Ala Asn 235 Phe Val Trp Leu Pro 240 Gly Glu Gly Ala Ala 245 Glu Leu Ala Ala Leu Ala Glu His Gly Ile 255 Val Ilie Arg Glu Glu Thr 275 Ala 260 Phe Pro Glu Gly Arg Ile Ser Val Thr Asn Ala 270 Asn Ala Gly Asp Lys Leu Leu Arg 280 Ala Trp Giu Ala Ile 285 <210> <211> <212> <213> <220> <221> <222> <223> 483 545

DNA

Corynebacteriun glutainicum

CDS

FRXA00446 <400> 483 atg gaa aag gtt cca aac gat gtc gtt Met Glu Lys Val Pro Asn Asp Val Val 1 5 ggg ctg gat gag Gly Leu Asp Glu gct tat Ala Tyr ttt gag ttc Phe Glu Phe cgc gcg gac gac Arg Ala Asp Asp cca gtt gcc act Pro Val Ala Thr gag gaa atc Glu Glu Ile aag gcg tat Lys Ala Tyr cac cgc cac gac aac gtg att His Arg His Asp Asn Val Ile ttg cgc acg ttc Leu Arg Thr Phe ggc ctg Gly Leu gcg ggc ttg cgt Ala Gly Leu Arg ggt tac gcc ttc Gly Tyr Ala Phe gga Gly aac gca gag atc Asn Ala Glu Ile 144 192 240 gca gcg atg aat Ala Ala Met Asn gtg gct att cct Val Ala Ile Pro gcg gtg aat tca Ala Val Asn Ser gct cag gcg gca gcg ctt gcg agt ttg Ala Gin Ala Ala Ala Leu Ala Ser Leu tct gcc gat gag Ser Ala Asp Glu ttg atg Leu Met gaa cgg gtg Glu Arg Val ctt ggt gct Leu Gly Ala 115 gag Glu 100 gaa acc gtc gaa Glu Thr Val Giu cgt gat gct gtg Arg Asp Ala Val gtg tca gcg Val Ser Ala 110 ccg ggc gag Pro Gly Glu gcg ccg acg cag Ala Pro Thr Gin aat ttc gtc tgg Asn Phe Val Trp ctg Leu 125 WO 01/00843 WO 0100843PCTIBOO/00923 ggc gcc gct gag ttg gcg gct aaa ttg Gly Ala Ala Glu Leu Ala Ala Lys Leu 130 135 cgc gcg ttc ccc gag ggt gcg cgc att Arg Ala Phe Pro Glu Gly Ala Arg Ile 145 150 act gac aag ctg ctg cgc gcg tgg gag Thr Asp Lys Leu Leu Arg Ala Trp Glu 165 tagtctttgg cgttttgcgg tgc <210> 484 <211> 174 <212> PRT <213> Corynebacteriun glutamicun <400> 484 Met Glu Lys Val Pro Asn Asp Val Val gcc gag cac ggc atc gtg att Ala Glu His Gly Ile Val Ile 140 tcg gtg acc aac gcc gag gaa Ser Val Thr Asn Ala Glu Glu 155 160 gcc atc aat gct ggg Ala Ile Asn Ala Gly 170 432 480 522 545 1 Phe His Gly Ile Al a Glu Leu Gly Arg 145 Thr Glu Arg Leu Ala Gln Arg Gly Ala 130 Ala Asp Gly Val1 Thr Phe Phe Ser

ASP

Val Glu Val 155 Ile Leu Ala Phe Gly Ala Ala Ala Trp His 140 Thr Asn <210> 485 <211> 1230 <212> DNA <213> Corynebacterium~ glutamicum WO 01/00843 WO 0100843PCT/JIBOO/00923 <220> <221> CDS <222> (101)..(1207) <223> RXS00618 gctgcattag agggtcgtat ctcgatctaa aagcagtacg gaagccaagc actagaagca atgttcagcc gtttcgcgtc cagataggct tgtctcttat atg cag atg ttg gac 11: Met Gin Met Leu Asp 1 cga gtc cac cgt Arg Val His Arg agg cgc gaa ggc Arg Arg Giu Gly gac acc tta atg Asp Thr Leu Met ttc tgc Phe Cys gct ggc cag ccg tca act ggt gcg Ala Gly Gin Pro Ser Thr Gly Ala gaa gca gtc atc Glu Ala Val Ile gaa gaa gca Glu Glu Ala gag atc gct Giu Ile Ala ctt cgc tcg ggt Leu Arg Ser Gly ttg gga tac acc gag gtg att ggt Leu Gly Tyr Thr Giu Val Ile Gly gat cgt Asp Arg gag ttc cgt gaa Giu Phe Arg Giu cgc atc gcc gat tgg Arg Ile Ala Asp Trp gac aat gtt att gtc Asp Asn-Val Ilie Val tct gct act tat Ser Ala Thr Tyr gta gac acc aac Val Asp Thr Asn acc acc ggt tct Thr Thr Gly Ser ggt gga ttc gtg Gly Gly Phe Val tcg ttt atc gcc Ser Phe Ile Ala ttg gat cac ggg Leu Asp His Gly gat tat Asp Tyr 100 gtg gca atg Val Ala Met tct ttg ggg Ser Leu Gly 120 cc t Pro 105 acc ccg ggg tac Thr Pro Gly Tyr gca tat cgc aat Ala Tyr Arg Asn att ctg gaa Ile Leu Giu 115 gag act cgt Giu Thr Arg gcg aag gtt ctg Ala Lys Val Leu ctg cgc tgt act Leu Arg Cys Thr gca Ala 130 ttc cag Phe Gin 135 cca acc gct caa atg ttg gag gaa ctg Pro Thr Ala Gin Met Leu Giu Giu Leu 140 cca Pro 145 cac aag ccg aag His Lys Pro Lys gtt att gtc acc Val Ile Val Thr cca gga aac cca Pro Gly Asn Pro ggc acc atc att Gly Thr Ile Ile ccg gaa gag cta Pro Glu Glu Leu cgc atc gcc aag Arg Ile Ala Lys tgc gat gac aat Cys Asp Asp Asn gat gct Asp Ala 180 gtt ctt atc Val Leu Ile tct Ser 185 gat gag gac tac Asp Giu Asp Tyr ggc atg agc ttt Gly Met Ser Phe ggt cgt ccg Gly Arg Pro 195 ctg gca act gcg cat cag ttt tcc aag aac gcc atc gtg gtg ggt acc WO 01/00843 Leu Ala Thr 200 ttg tcc aag Leu Ser Lys 215 gtt cca gat Val Pro Asp 230 tcc ttg tgt Ser Leu Cys act ttg gag Thr Leu Glu gag gcc cgg Glu Ala Arg 280 act ttc gcc 1027 Thr Phe Ala 295 gca tac acc 1075 Ala Tyr Thr 310 gcg ggc gtg 1123 Ala Gly Val cac aag tgg 1171 His Lys Trp gaa ggt gtg 1217 Giu Gly Val 360 Ala tac Tyr gag Giu gc t Ala gct Ala 26 gag Glu gac Asp gat Asp gcc Ala att Ile 345 cgc Arg His t tc Phe c tg Leu cct Pro 250 ggg Gly gtg Val ccg Pro gat Asp gtc Val 330 cgt Arg aaa Lys Ser Lys 205 acg ggt Thr Gly ccg att Pro Ile ggg cag Gly Gin ctt gat Leu Asp 270 gat aag Asp Lys 285 ggc ctg Gly Leu gaa tgg Glu Trp ggt gtt Gly Val ctg tgc Leu Cys 350 gaa ttc Glu Phe 365 Ala cgc Arg aac Asn 240 gcg Ala cac His cct Pro ttg Leu t tg Leu 320 ttt Phe tca Ser aaa Lys Ile gtg Val 225 ctg Leu gga Gly gtt Val gaa Glu tgg Trp 305 cgt Arg gat Asp aag Lys aaa Lys Val Val Gly 210 ggt tgg atc Gly Trp Ile cag gct tct Gin Ala Ser cgc gca gcc Arg Ala Ala 260 gaa gcg tat Glu Ala Tyr 275 atc ggg ctt Ile Gly Leu 290 gtc gat gtt Val Asp-Val ttg ctc gat Leu Leu Asp cct gag gaa Pro Glu Glu 340 gaa gac acc Glu Asp Thr 355 tagcagcgac CTA1B00100923 Thr atc 787 Ile ctt 835 Leu 245 ttc 883 Phe cgc 931 Arg ggc 979 Gly tct Ser gaa Glu 325 ggc Gly att Ile taggttagtt tcg 1230 <210> 486 <211> 369 <212> PRT <213> Corynebacterium giutainicum <400> 486 Met Gin Met Leu Asp Arg Val His Arg Arg Arg Arg Glu Gly Lys ASP 1 5 10 WO 01/00843 Thr Leu Met Val Ile Glu Thr Glu Val His Ser Ala Thr Thr Gly Asp His Gly Arg Asn Ile 11*5 Thr Ala Giu 130 Pro His Lys 145 Gly Thr Ile Asp Asp Asn Ser Phe Gly 195 Ile Val Val 210 Vai Giy Trp 225 Leu Gin Ala Gly Arg Ala Vai Giu Ala 275 Glu Ile Gly 290 Trp Val Asp 305 Arg Leu Leu Asp Pro Giu Phe Glu Ile Thr Ser Asp 100 Leu Thr Pro Ile Asp 180 Arg Gly Ile Ser Ala 260 Tyr Leu Val Asp Glu Cys Al a Giy Tyr Ser Tyr Giu Arg Lys Asp 165 Al a Pro Thr Ile Leu 245 Phe Arg Giy Ser Giu 325 Gly Ala Giu Asp Asp 70 Giy Vai Ser Phe Ala 150 Pro Val1 Leu Leu Val 230 Ser Thr Glu Thr Ala 310 Ala His Gly Ile Arg 55 Vai Giy Aia Leu Gin 135 Val Giu Leu Aia Ser 215 Pro Leu Leu Aia Phe 295 Tyr Giy Lys Gin Al a Giu Asp Phe Met Gly 120 Pro Ile Glu Ile Thr 200 Lys Asp Cys Giu Arg 280 Aia Thr Val Trp Pro Leu Phe Thr Vai Pro 105 Ala Thr Vai Leu Ser 185 Ala Tyr Giu Ala Ala 265 Giu Asp Asp Aia Ile Ser Arg Arg Asn Ala 90 Thr Lys Aia Thr Giu 170 Asp His Phe Leu Pro 250 Gly Vai Pro Asp Val 330 Arg Thr Ser Giu Pro 75 Ser Pro Val Gin Ser 155 Arg Giu Gin Ser Vai 235 Ala Ala Phe Asp Ser 315 Ala Leu Gly Gly Arg Asp Phe Gly Leu Met 140 Pro Ile Asp Phe Met 220 Thr Ile Glu Val Gly 300 Glu Pro Ser PCTJIBOO/00923 Giu Ala Gly Tyr Asp Trp, Ile Val Thr Leu Ala TPyr Arg Cys Glu Leu Pro Thr 160 Trp Cys 175 Gly Met Asn Ala Trp Arg Glu Asn 240 Ala Ala 255 Ala His Leu Pro Tyr Leu Ala Leu 320 Asp Phe 335 Ala Ser WO 01/00843 WO 0100843PCTIIBOO/00923 345 Lys Glu ASP Thr Ile Giu Gly Val Arg Lys Ile Gly Glu Phe Ile Lys 355 360 365 Lys <210> 487 <211> 657 <212> DNA <213> Corynebacteriun glutamicum <220> <221> <222> <223>

CDS

(101). (634) FR.XA00618 <400> 487 cccaacgggc accatcattg atccggaaga gctagagcgc atcgccaagt ggtgcgatga caatgatgct gttcttatct ctgatgagga ctaccacggc atg Met 1 agc ttt ggt Ser Phe Gly ccg ctg gca act gcg cat cag ttt tcc Pro Leu Ala Thr Ala His Gin Phe Ser aac gcc atc gtg Asn Ala Ile Val gtg ggt Val Gly acc ttg tcc Thr Leu Ser tac ttc tcc atg Tyr Phe Ser Met ggt tgg cgc gtg Gly Trp Arg Val ggt tgg atc Gly Trp Ile cag gct tct Gin Ala Ser atc gtt cca gat gag ctg gtc Ilie Val Pro Asp Glu Leu Val ccg att gaa aac Pro Ile Glu Asn ctt tcC Leu Ser ttg tgt gct cct Leu Cys Ala Pro atc ggg cag gct Ile Gly Gin Ala gcg Ala gga cgc gca gcc Gly Arg Ala Ala ttC Phe act ttg gag gct Thr Leu Glu Ala gcc gaa ctt gat Ala Giu Leu Asp cac gtt gaa gcg His Val Glu Ala cgc gag gcc cgg Arg Glu Ala Arg gtg ttc gtc gat Val Phe Val Asp ctc cct gaa atc Leu Pro Glu Ile ggg ctt Gly Leu 100 ggc act ttc Gly Thr Phe tct gca tac Ser Ala Tyr 120 gac ccg gat ggc Asp Pro Asp Gly ctg tat ttg tgg Leu Tyr Leu Trp gtc gatgtt Val Asp Val 115 ttg ctc gat Leu Leu Asp acc gat gat tca Thr Asp Asp Ser gaa tgg gca ttg Glu Trp Ala Leu gaa gcg Glu Ala 135 ggc gtg gcc gtc Gly Val Ala Val gcg Ala 140 ccg ggt gtt gat Pro Gly Val Asp ttt Phe 145 gat cct gag gaa Asp Pro Glu Glu WO 01/00843 WO 0100843PCTIBOO/00923 ggc cac aag tgg att cgt ttg agc ctg tgc gCg tca aag gaa gac acc Gly His Lys Trp Ile Arg Leu Ser Leu Cys Ala Ser Lys Glu Asp Thr 150 155 160 165 att gaa ggt gtg cgc aaa atc gga gaa ttc atc aaa aaa tagcagcgac Ile Glu Gly Val Arg Lys Ile Gly Glu Phe Ile Lys Lys 170 175 taggttagtt tcg <210> 488 <211> 178 <212> PRT <213> Corynebacterium glutamicun <400> 488 Met Ser Phe Gly Arg Pro Leu Ala Thr 1 Ala Arg Asn Ala His Pro Leu Leu Phe 145 Ser Lys Val1 Gly Gin Arg Glu Ile Val 115 Leu Pro Glu Val Trp Ala Ala Ala Gly 100 Asp Leu Giu Asp Thr Ilie Leu Phe 70 Arg Gly Ser Giu Gly 150 Ile Leu Val1 Ser Thr Glu Thr Ala Ala 135 His Glu Ser Pro 40 Leu Leu Al a Phe Tyr 120 Gly Lys Gly Ala 10 Tyr Glu Ala Ala Glu Asp Asp Ala Ile Arg 170 His Phe Leu Pro Gly 75 Val1 Pro Asp Val Arg 155 Lys Gln Ser Val1 Ala Ala Phe Asp Ser Ala 140 Leu Ile Phe Met Thr Ile Glu Val Gly Glu 125 Pro Ser Gly Lys Gly Ile Gin Asp Lys Leu Trp Val Cys Phe 175 Asn Trp Giu Ala Ala Leu Tyr Ala Asp Ala 160 Ile <210> 489 <211> 385 <212> DNA <213> Corynebacterium glutanicun <22 0> WO 01/00843 <221> CDS <222> (101)..(385) <223> FRXA00627 <400> 489 gctgcattag agggtcgtat ctcgatctaa aagcagtacg gaagccaagc actagaagca atgttcagcc gtttcgcgtc PCT11B00100923 cga Arg gc t Al a gag Glu ga t Asp gac Asp ggt Gly agg Arg act Thr tcg Ser gaa Glu cct Pro 75 tcg Ser cgc Arg ggt Gly gg t Gly cgc Arg gac Asp ttt Phe aaa Lys gaa Glu gga Gly gat Asp at t Ile acc Thr Arg 10 Ser Arg Arg Asn Ala 90 gac Asp gca Ala tac Tyr tgg Trp gtc Val 80 Arg Thr Ser Glu Pro 75 Ser cagataggct tgtctcttat atg cag atg ttg gac Met Gln Met Leu Asp 1 acc tta atg ttc tgc Thr Leu Met Phe Cys gtc atc gaa gaa gca Val Ile Glu Glu Ala acc gag gtg att ggt Thr Glu Val Ile Gly cac tct gct act tat His Ser Ala Thr Tyr acc acc ggt tct tca Thr Thr Gly Ser Ser <210> 490 <211> <212> PRT <213> Corynebacteriun glutaiicun <400> 490 Met Gln Met Leu Asp Arg Val His I 1 5 Thr Leu Met Phe Cys Ala Gly Gln 1 Val Ile Glu Glu Ala Glu Ile Ala 1 Thr Glu Val Ile Gly Asp Arg Glu 1 His Ser Ala Thr Tyr Asp Val Asp 70 Thr Thr Gly Ser Ser Gly Gly Phe 1 Gly Pro Leu Ala Val1 Ala Lys Asp Glu Ala Gly Tyr Asp Trp Ile Val Thr WO 01/00843 WO 0100843PCTIBOOIOO923 <210> 491 <211> 1221 <212> DNA <213> Corynebacterium glutamicun <220> <221> <222> <223>

CDS

(101)..(1198) RXS0 1105 <400> 491 ttgtcatcaa ctttgccaac gccgaagatc ttccagcgca cggcgaagca atccgtgcac gctttgaaaa cctccccacc accgacgagg cctaage ttg agc gat ttg cca ttg cgt gaa gaa ctg Leu Ser Asp Leu Pro Leu Arg Giu Glu Leu 15 ~aaa atg acc aaa att Met Thr Lys Ile 1 cgc ggt gag cac gct Arg Gly Giu His Ala tac Tyr ggc gca ccc cag ctc aac Gly Ala Pro Gin Leu Asn gtt gat att Val Asp Ile cgc ctc aac acc Arg Leu Asn Thr aac gaa aac Asn Giu Asn gcc acc gtg Ala Thr Val cct tac cca Pro Tyr Pro ccg tca gag gca Pro Ser Glu Ala ttg gtc Leu Val gct gac ttg Ala Asp Leu gat aag atc gcc acc gag Asp Lys Ile Ala Thr Glu aac cgc tac cca Asn.Arg Tyr Pro gag Glu cgc gat gct gtg Arg Asp Ala Val 211 259 307 355 403 gaa Glu ctg cgt gat gag Leu Arg Asp Giu gct gcg tac atc Ala Ala Tyr Ile aag caa acc ggc Lys Gin Thr Gly gct gtc acc agg Ala Val Thr Arg aac ctg tgg gct Asn Leu Trp Ala aat ggt tcc aat Asn Gly Ser Asn gaa att Glu Ile 100 ctg cag cag Leu Gin Gin gga ttc caa Gly Phe Gin 120 ctg cag gct tC Leu Gin Ala Phe ggt Gly 110 gga cct gga cgc Gly Pro Gly Arg acc gcg ttg Thr Ala Leu 115 aaa ggc acc Lys Gly Thr ccc agc tat tcc Pro Ser Tyr Ser cac cca att ttg His Pro Ile Leu cac act His Thr 135 gaa ttc ate gcg gtg tcc cga ggt gct Glu Phe Ile Ala Val Ser Arg Gly Ala 140 ga t Asp 145 ttc cgc atc gat Phe Arg Ile Asp atg Met 150 gat gtg gcg ctg Asp Val Ala Leu gaa att cgt gca Glu Ile Arg Ala cag cct gac at Gin Pro Asp Ile tc gtc acc acc Phe Val Thr Thr ccg Pro 170 aac aac ccg acc Asn Asn Pro Thr ggt gat Gly Asp 175 gtg acc tcg Val Thr Ser ctg gac Leu Asp 180 WO 01/00843 gat gtt gag cgc atc atc aac gtt gcc Asp Val Glu Arg Ile Ile Asn Val Ala 185 190 gaa gct tat gcg gaa ttc tcc cca tca Giu Ala Tyr Ala Glu Phe Ser Pro Ser 200 205 gag aag tac cca acc aag ctg gtg gtg Giu Lys Tyr Pro Thr Lys Leu Val Val 215 220 ttt gat ttc gca ggt gga cgc ctc ggc Phe Asp Phe Ala Gly Gly Arg Leu Gly 230 235 ttt atc gac gcc gtg atg cta gtc cgc Phe Ilie Asp Ala Val Met Leu Val Arg 250 ctg agc caa gca gcc gca atc gta gcg Leu Ser Gin Ala Ala Ala Ile Val Ala 265 270 ctg gga acc gtc gaa aag ctc tct gta Leu Giy Thr Val Glu Lys Leu Ser Val 280 285 cgc ttg gag gaa ctg ggc tac gct gtg 1027 Arg Leu Glu Giu Leu Gly Tyr Ala Val 295 300 gtg ttc ttt gga gat ttc tcc gat cag 1075 Val Phe Phe Gly Asp Phe Ser Asp Gin 310 315 ttg gat agg gga gtg ctc atc cgc gat 1123 Leu Asp Arg Gly Val Leu Ile Arg Asp 330 cgc act acc att ggt gtg cct gag gaa 1171 Arg Thr Thr Ile Gly Val Pro Glu Giu 345 350 gct gca gag atc atc aag ctg aac ctg 1218 Ala Ala Glu Ile Ilie Lys Leu Asn Leu 360 365 tga 1221 <210> 492 <211> 366 <212> PRT <213> Corynebacteriun glutainicum PCTIBOOIOO923 cca ggc atc gtg atc gtg gat 691 Pro Gly Ile Val Ile Val Asp 195 cct tca gca acc act ctt ctg 739 Pro Ser Ala Thr Thr Leu Leu 210 tcc cgc acc atg agt aag gct 787 Ser Arg Thr Met Ser Lys Ala 225 tac ttc gtg gcc aac cca gcg 835 Tyr Phe Val Ala Asn Pro Ala 240 245 ctt ccg tat cat ctt tca gcg 883 Leu Pro Tyr His Leu Ser Ala 255 260 ctg cgt cac tcc gct gac acg 931 Leu Arg His Ser Ala Asp Thr 275 gag cgt gtt cgc gtg gca gca 979 Giu Arg Val Arg Val Ala Ala 290 gtt cca agt gag tcc aac ttt Val Pro Ser Giu Ser Asn Phe 305 cac gcg gca tgg cag gca ttt His Ala Ala Trp Gin Ala Phe 320 325 gtg gga atc gct ggg cac ttg Val Gly Ile Ala Gly His Leu 335 340 aat gat gcg ttt ttg gac gca Asn Asp Ala Phe Leu Asp Ala 355 taagagagaa gaatttttca WO 01/00843 <400> 492 Met Thr Lys PCTlIBOO/00923 Ile Thr Leu Ser Asp Leu Pro Leu Arg Giu Giu Leu Arg 1 5 10 Gly Asn Leu Glu Lys Giy Gly Leu Asp 145 Gin Val Ile Ala Thr 225 Val Tyr His Val1 Ser 305 Giu Thr Val Arg Gin Ser Arg Ala 130 Phe Pro Thr Val1 Thr 210 Met Ala His Ser Arg 290 Giu His Asn Ala Asp Thr Asn Thr 115 Lys Arg Asp Se r Ile 195 Thr Ser Asn Leu Ala 275 Val1 Ser Ala Giu Thr Ala Gly Glu 100 Ala Gly Ile Ile Leu 180 Val Leu Lys Pro Ser 260 Asp Ala Asn Tyr Asn Val Val Val Ile Leu Thr Asp Val 165 Asp Asp Leu Ala Ala 245 Ala Thr Al a Phe Gin 25 Pro Ala Asp Arg Leu 105 Pro Phe Ala Thr Arg 185 Al a Pro Ala Al a Ala 265 Val Glu Gly Leu Ser Thr Giu Asp Leu Ser Ile Leu Pro 170 Ile Giu Thr Gly Val 250 Ala Giu Leu Asp Asn Glu Glu Leu 75 Asn Gin Tyr Ala Giu 155 Asn Ile Phe Lys Gly 235 Met Ala Lys Gly Phe 315 Val1 Ala Leu Ala Leu Ala Ser Val 140 Glu Asn Asn Ser Leu 220 Arg Leu Ile Leu Tyr 300 Ser Ile Val Arg Tyr Ala Gly 110 His Arg Arg Thr Ala 190 Ser Val Gly Arg Ala 270 Val Val1 Gin Leu Asp Pro Thr Asn Pro Ile Ala Lys 160

ASP

Gly Ser Arg Phe 240 Pro Arg Arg Pro Al a 320 WO 01/00843 WO 0100843PCTIiBOO/00923 Ala Trp Gin Ala Phe Leu Asp Arg Gly Val Leu Ile Arg Asp Val Gly 325 330 335 Ilie Ala Giy His Leu Arg Thr Thr Ile Gly Vai Pro Giu Giu Asn Asp 340 345 350 Ala Phe Leu Asp Ala Ala Ala Glu Ile Ile Lys Leu Asn Leu 355 360 365 <210> 493 <211> 1752 <212> DNA <213> Corynebacterium giutainicum <220> <221> CDS <222> (i01)..(1729) <223> RXS02315 <400> 493 cgtttggaaa cgcttgctgc cagcaaagat aggcgtgatt ggtggtttga gcgcgtgcgt gaatcgtatc cgtacctgga gacgatctag actgttgtgc atg tcc agc acg cca Met Ser Ser Thr Pro gc t Ala act Thr gag Giu cgc Arg c cg Pro cc t Pro tct Ser att Ile caa Gin gac Asp ttg Leu agc Ser gtg Vai gct Aia gc t Ala aac Asn gat Asp gtg Val c tg Leu gcc Ala gc t Al a gt t Vai gac Asp cag Gin 120 cgc Arg t gc Cys cag Gin ttg Leu atg Met 75 gct Aia gca Ala att Ile gcc Ala cca Pro gat

ASP

gcg Ala 60 acc Thr gcg Ala cat His ttt Phe gat Asp agg Arg gtc Vai cta Leu acg Thr cat His gga Gly ctt Leu tcc Ser aac Asn cat His gcg Ala gct Ala atc Ile acg Thr gca Ala 1 c tc Leu tca Ser gtg Val cgt Arg gta Val ccg Pro ggg Gly ccg Pro cca Pro t tg Leu atc Ile cag Gin aac Asn at t Ile agc Ser 115 gtc Val1 cac His tcg S er gac Asp gcc Ala tgc Cys gtg Val1 100 caa Gin ggt Gly act gag ctg gat cag gta gcg cag att gct gaa agc ctt gct cag ggg WO 01/00843 Thr Giu Le 135 gct tcc ca Ala Ser GI 150 gtt gct cc Val Ala Px tgg acg ca Trp Thr Hi gag cac ac Glu His TY, 2C ctg gaa ga Leu Glu As 215 aat ccg gt Asn Pro Ve 230 tcc gcg ga Ser Ala G2 gtg gga ca Val Gly Hi cct ggc at Pro Gly I] 2E ccc ggc c 1027 Pro Gly Az 295 acc cag ge 1075 Thr Gin G] 310 gcg gCC ge 1123 Ala Ala As 8CC ggc ct 1171 Thr Gly Le gat act ct 1219 Asp att Ile gaa Glu cgc Arg 185 ctc Leu gtg Val cac His ggc Gly

CCC

Pro 265 888 Lys cat His aag Lys ggt: Gly cat His 345 ttt Gin ccg Pro c tg Leu 170 tgg Trp gtg Val

CCC

Pro cca Pro tat Tyr 250 acg Thr tta Leu gCC Ala Cag Gin gtg Val 330 gt t Val gc t Val cgt Arg 155 cca Pro atc Ile att Ile aCC Thr Ctg Leu 235 gtg Val Ctg Leu act Thr gat Asp tgg Trp 315 cgt Arg gcc Ala gca Ser 145 ctt Leu gca Ala 8CC Thr tgg Trp gca Ala 225 ctg Leu gat Asp aag Lys gat Asp gtg Val1 305 gca Ala C 88 Gin 8CC Thr cgt Leu gat Asp gtt Val g tg Val gaa Glu 210 cca Pro aag Lys cat His ttg Leu atc Ile 290 888 Lys gca Ala gaa Glu tta Leu gac PCTIBOO/00923 Gin Gly cct ttg 595 Pro Leu 165 gca tca 643 Ala Ser 180 ctg ggg 691 Leu Gly gaa ggg 739 Giu Gly cct tat 787 Pro Tyr cag gtc 835 Gin Val 245 atc gtg 883 Ile Val 260 tca gat 931 Ser Asp act gat 979 Thr Asp aCC ggc Thr Gly gaa ctt Glu Leu 325 ggt ttC Gly Phe 340 acc ggc Thr Gly tcc ctg WO 01/00843 Asp Thr Leu 360 gtg ggt atg 1267 Val Gly Met 375 gca ggc att 1315 Ala Gly Ilie 390 gtg cag tcc 1363 Val Gin Ser ctt ctg ggc 1411 Leu Leu Gly ggc cct gat 1459 Gly Pro Asp 440 gac aac ggt 1507 Asp Asn Gly 455 ctc cgc ccc 1555 Leu Arg Pro 470 atc gcg gat 1603 Ilie Ala Asp aac ctc caa 1651 Asn Leu Gin gga ttc acc 1699 Gly Phe Thr 520 caa cag caa 1749 Gin Gin Gin 535 gcg 1752 Phe cc t Pro gat Asp cgc Arg ga t Asp 425 gaa Giu Gly aac Asn ctc Leu gac Asp 505 att Ile gc t Ala Ser Asn Ser 365 gtg gat acc Vai Asp Thr gct caa gca Aia Gin Ala gaa atc cgc Glu Ile Arg 415 ctt cac gat Leu His Asp 430 gaa aac ctc Giu Asn Leu 445 gaa ctc cta Giu Leu Leu gct ttc ggt Aia Phe Giy tac ggc att Tyr Gly Ile 495 gcg cta gtt Aia Leu Val 510 tcg acc gtc Ser Thr Val 525 acg gtg cac Thr Vai His Ile ttc Phe atc Ile 400 gcg Ala att Ile acc Thr gaa Glu acc Thr 480 gaa Glu gat Asp cga Arg t cc -Ser 385 ggc Gly cca Pro ggc Gly atc Ile acc Thr 465 cca Pro cac His acc Thr gat PCTIIBOO/00923 Ser Leu ggt gtc Gly Vai ctt gct Leu Ala 405 gtg gcc Val Ala 420 ctc atc Leu Ile tcc aac Ser Asn gat ggt Asp Gly gcg tcc Ala Ser 485 gta gac Val Asp 500 gta tcc Vai Ser cgt gca Arg Asp Thr Arg Arg Ala 530 taaatggagt ggtaccaagt WO 01/00843 WO 0100843PCT/LLBOO/00923 <210> 494 <211> 543 <212> PRT <213> Corynebacteriun giutaxnicum <400> 494 Met Ser Ser Thr Pro Ala Gin Asp Leu Ala Arg Ala Val Ile Asp Ser 1 5 10 Leu Ala Pro His Val Thr Asp Val Val Leu Cys Pro Gly Ser Arg Asn 25 Ser Pro Leu Ser Leu Giu Leu Leu Ala Arg Gin Asp Leu Arg Val Hius 40 Val Arg Ile Asp Glu Arg Ser Ala Ser Phe Leu Ala Leu Ser Leu Ala 55 Arg Thr Gin Ala Arg Pro Val Ala Val Val Met Thr Ser Gly Thr Ala 70 75 Val Ala Asn Cys Leu Pro Ala Val Ala Giu Ala Ala His Ala His Ile 90 Pro Leu Ile Val Leu Ser Ala Asp Arg Pro Ala His Leu Val Gly Thr 100 105 110 Gly Ala Ser Gin Thr Ile Asn Gin Thr Gly Ile Phe Gly Asp Leu Ala 115 120 125 Pro Thr Val Gly Ile Thr Giu Leu Asp Gin Val Alai Gin Ile Ala Giu 130 135 140 Ser Leu Ala Gin Gly Ala Ser Gin Ile Pro Arg His Phe Asn Leu Ala 145 150 155 160 Leu Asp Val Pro Leu Val Ala Pro Giu Leu Pro Giu Leu His Gly Giu 165 170 175 Ala Val Gly Ala Ser Trp Thr His Arg Trp Ile Asn His Gly Giu Val 180 185 190 Thr Val Asp Leu Gly Giu His Thr Leu Val Ile Ala Gly Asp Giu Ala 195 200 205 Trp Giu Val Giu Gly Leu Giu Asp Val Pro Thr Ile Ala Giu Pro Thr 210 215 220 Ala Pro Lys Pro Tyr Asn Pro Val His Pro Leu Ala Ala Giu Ile Leu 225 230 235 240 Leu Lys Giu Gin Val Ser Ala Giu Gly Tyr Val Val Asn Thr Arg Pro 245 250 255 Asp His Val Ile Val Val Gly His Pro Thr Leu His Arg Gly Val Leu 260 265 270 Lys Leu Met Ser Asp Pro Gly Ile Lys Leu Thr Val Leu Ser Arg Thr 275 280 285 WO 01/00843 Asp Ile Ile 290 Val Lys Val 305 Ala Ala Ser Gin Giu Phe Thr Leu Gly 355 Arg Asp Leu 370 Ser Pro Arg 385 Gly Thr Ser Pro Arg Thr Giy Gly Leu 435 Ilie Val, Val 450 Thr Gly Aia 465 Pro His Asp His Gin Val Thr Thr Giu 515 Asp Thr Arg 530 Gly 295 Gin Ala Gly Thr Gly 375 Gly Gin Leu Pro Asn 455 Arg Ala Leu Phe Gin 535 Arg Giu Asp Leu Leu 360 Met Ile Ser Gly Asp 440 Gly Pro Asp Gin Thr 520 Gin His Lys Gly His 345 Phe Pro Asp Arg Asp 425 Giu Gly Asn Leu Asp 505 Ile Ala Ala Gin Val1 330 Val Ala Phe Gly His 410 Leu Pro Gly Phe Cys 490 Leu Ile Leu Asp Trp 315 Arg Al a Ala Asp Ser 395 Pro Ser Arg Ile Giu 475 Ala Ile Giu Met Gly Ile Leu Val1 350 Asn Asp Gin Ile His 430 Asn Leu Phe Gly Leu 510 Thi7 Val PCTIIBOO/00923 Ser Thr Cys Ser 320 Asp Asn 335 Ala Asp Ser Ile Thr Phe Ala Ile 400 Arg Ala 415 Asp Ile Leu Thr Leu Giu Gly Thr 480 Ile Giu 495 Val Asp Val Arg His <210> 495 <211> 1434 <212> DNA <213> Corynebacterium giutamicum <22 0> <221> CDS <222> (101)..(1411) <223> RXS02550 <400> 495 gatcttaggc agccgtggga ttacaccctt ttagagctag aacagtaaaa attcacccaa WO 01/00843 WO 0100843PCTflBOO/00923 tagctttcaa ctacgcacac aaagtggcaa cattgagcgg cgc Arg gat

ASP

cag Gin gcc Ala ctc Leu att Ile acc Thr gaa Giu Gly aac Asn 150 act Thr gat Asp atc Ilie acg Thr aaa Lys cag Gin tcg Ser gcg Ala aac Asn atg Met t cC Ser gtt Vai aac Asn 135 gac Asp gcc Ala gag Glu tca Ser gga Gly 215 acc Thr gca Ala gag Giu gag Giu acg Thr cgt Arg aaa Lys gtg Val 120 ggt Gly ggc Gly gca Ala gaa Glu gag Giu 200 gc t Ala tct Ser gcg Ala aag Lys gcg Ala gga Gly gac Asp ggc Gly 105 ccc Pro gtc Val gat Asp ac c Thr gat Asp 185 aaa Lys gtc Val1 aag Lys cg t Arg atg Met gaa Giu aat Asn atg Met att Ilie gga Gly tca Ser gaa Giu t cc Ser 170 gac Asp acc Thr tac Tyr ac c Thr ccc Pro aag Lys cgc Arg cca Pro atc Ile att Ile t tc Phe gaa Giu gt t Val 155 ctg Leu tgg Trp aaa Lys ccg Pro acc Thr act Thr gac Asp atg Met gcc Ala gcc Ala ccg Pro ccc Pro cta Leu 140 ctt Leu gct Ala aac Asn gc t Ala cgc Arg 220 act aca gac Thr Thr Asp gct gtg ggc Ala Val Gly cgc atc ttc Arg Ile Phe cgt ggc ccg Arg Gly Pro aac atc tta Asn Ile Leu gcc ccc gac Ala Pro Asp caa ggg tat Gin Gly Tyr 100 gtc acc cgc Val Thr Arg 115 gat gtg ttc Asp Val Phe 130 caa gca ctc Gin Ala Leu tac cca ctg Tyr Pro Leu cac tac ctc His Tyr Leu 180 atc aag tcc Ile Lys Ser 195 ccc aac aac Pro Asn Asn 210 atc gtc gag Ile Val Giu WO 01/00843 WO 0100843PCTIBOOIOO923 gca cgc Ala Arg 230 gag cat gac Glu His Asp ctg Leu 235 gcc Al a ctg att ttg gcc Leu Ile Leu Ala gaa atc tac gac Giu Ile Tyr Asp cgc Arg 245 att ctc tac gat Ile Leu Tyr Asp gat Asp 250 gag cac atc Giu His Ile gca acc ctt Ala Thr Leu gca cca Ala Pro 260 cgc gtc Arg Val gat ctC ctt Asp Leu Leu gca gga tac Ala Gly Tyr 280 tgc Cys 265 cga Arg atc aca tac aac Ile Thr Tyr Asn tcc aag gca Ser Lys Ala tac Tyr 275 aag Lys gct ggc tgg Ala Gly Trp ttg act gga Leu Thr Gly cca Pro 290 caa tac 979 Gin Tyr gca cgt 1027 Ala Arg 295 tgc cca 1075 Cys Pro 310 cgc cag 1123 Arg Gin cag cgc 1171 Gin Arg tgt gtg 1219 Cys Val aac gtg 1267 Asn Val 375 cgt gcc 1315 Arg Ala 390 cat cac 1363 His His gaa aac 1411 Giu Asn gga ttt att gag Gly Phe Ilie Giu aat gtc cca gct Asn Val Pro Ala 315 tcc atc tac gac Ser Ile Tyr Asp 330 aac atg gca tgg Asn Met Ala Trp gaa ctc ctc gca ggc act cga ctc Giu Leu Leu Ala Gly Thr Arg Leu 305 gct att cag gta gct ctg ggt gga Ala Ile Gin Val Ala Leu Giy Giy 320 325 cag cac Gin His ctc act ggc gaa cac Leu Thr Gly Giu His 335 ggc cga ctc ctg gaa Gly Arg Leu Leu Giu 340 acg aaa Thr Lys 345 aaa cca Lys Pro atg gga gct cta Met Gly Ala Leu 365 atc cac gac gac Ilie His Asp Asp ctc aac gaa atc cc& ggt gtc agc Leu Asn Giu Ile Pro Giy Val Ser 350 355 tac gcg ttc ccc aag ctc gac ccc Tyr Ala Phe Pro Lys Leu Asp Pro 370 acc caa ctc atg ctg gat ctt ctc Thr Gin Leu Met Leu Asp Leu Leu 385 cag ggc act ggc ttc aac tgg cca Gin Gly Thr Gly Phe Asn Trp Pro gaa Glu 380 gag aaa atc ctc GiU Lys Ile Leu 395 gat cac ttc cga Asp His Phe Arg 410 gca att gag cgc Ala Ile Giu Arg 425 atg Met gtt Val 400 405 gtg gtc acc ctg Val Vai Thr Leu 415 ctg ggt aac ttc Leu Gly Asn Phe 430 cca tgg gca tc cag ttg Pro Trp Ala Ser Gin Leu 420 ctg tcc act tao aag cag Leu Ser Thr Tyr Lys Gin 435 WO 01/00843 tagtagttgt taggattcac cac 1434 <210> 496 <211> 437 <212> PRT <213> Corynebacterium giutamicum <400> 496 Val Thr Thr Asp Lys Arg Lys Thr Ser PCT/BOOIOO923 1 Lys Arg Ile His Asp Ser Val Asp Thr 145 Asp Val1 Asp Asn Gin 225 Giu Ala Vali Ile Giy Ile Pro Gly Thr 115 Val1 Ala Pro Tyr Lys 195 Asn Vali Tyr Leu Giy Phe Pro Leu Asp 100 Arg Phe Leu Leu Leu 180 Ser Asn Giu Asp Ala 260 5 Ala Asp Val Lys Val Ser Tyr Leu Leu Trp 165 Cys Lys Pro Ile Arg 245 Pro Asp Gin Ala Leu 70 Ile Thr Glu Gly Asn 150 Thr Asp Ile Thr Ala 230 Ile Gin Ser Ala Asn Met Ser Vai Asn 135 Asp Ala Glu Ser Gly 215 Arg Leu Ala Gi u Giu Thr Arg Lys Val1 120 Gly Gly Ala Giu Glu 200 Aia Giu Tyr Lys 10 Arg Met Giu Asn Met 90 Ile Giy Ser Giu Ser 170 Asp Thr Tyr Asp

ASP

250 Thr Thr Asp Met Aia Ala Pro Pro Leu 140 Leu Ala Asn Ala Arg 220 Leu Glu Thr Arg Leu Leu Phe Leu Arg 110 Phe Thr Pro Gly Ser 190 Val Vai Leu Ile Ala Thr Tyr Asp Gly Pro Arg Asp Met Ala Lys 175 Ile Val Leu Ala Ser Asn Thr Giu Gly Phe Thr Ala Val Thr Pro 160 Pro Giu Ile Giu

ASP

240 Leu 255 Asp Leu Leu Ile Thr Tyr Asn Gly Leu Ser 270 WO 01/00843 Lys Ala Ty 27 Giy Pro Ly 290 Ala Gly Th 305 Val Ala Le Giy Arg Le Ile Pro Gi Pro Lys LE 370 Met Leu As 385 Gly Phe As Trp Ala Se Ser Thr Tv' Arg Val Gin Tyr Arg Leu Gly Gly 325 Leu Giu 340 Val Ser Asp Pro Leu Leu Trp Pro 405 Gin Leu 420 Lys Gin Ala Ala Cys 310 Arg Gin Cys Asn Arg 390 His Giu Tyr 280 Giy Asn Ser Asn Lys 360 Tyr Glu Asp Ala Arg Phe Val Ilie Met 345 Pro Giu Lys His Ile 425 PCTIiBOOIOO923 Leu Thr Leu Leu Ile Gin 320 Giu His 335 Asn Giu Ala Phe Gin Leu Giy Thr 400 Leu Pro 415 Phe Leu <210> 497 <211> i080 <212> DNA <213> Corynebacterium giutainicum <220> <221> CDS <222> (101)..(1057) <223> RXS02319 <400> 497 atgtgggtga gataaccgac cgtgatgtcg ccctagcaaa agaccttggc catttcggca gaggcttaag gttaaagatt acc gac aac cct ttt gat ccc acc caa tgg gcc Thr Asp Asn Pro Phe Asp Pro Thr Gin Trp Ala 15 gaa gaa ttc acc gac atc acc tac cac cgc cac Giu Giu Phe Thr Asp Ilie Thr Tyr His Arg His 30 gcc gat ggc atc gtg cgc atc gcc ttc gac cgc Ala Asp Gly Ile Val Arg Ile Ala Phe Asp Arg agtcatcgac gcccacgcca atg agc aac tac agc Met Ser Asn Tyr Ser 1 acc gtt cca ggt ttt Thr Val Pro Gly Phe gtg ggc acc acc cgc Vai Giy Thr Thr Arg ccc gaa gtt cgc aat Pro Giu Vai Arg Asn 115 163 211 259 WO 01/00843 WO 0100843PCTIIBOOIOO923 gc t Ala gcg Ala

CCC

Pro cgc Arg gac Asp gtt Val 8CC Thr 150 ggt Gly caa Gin gct Ala gcc Ala caa Gin 230 aaa Lys ggg Gly atg Met ttc Phe cgc Arg agc Ser atc Ile ga t Asp gaa Giu 135 atg Met ggg Gly gaa Giu ggc Gly cgc Arg 215 caa Gin gaa Giu caa Gin gga Giy cgc Arg cgg Arg gaa Glu cgc Arg gcc Ala 120 ggc Gly CC t Pro cac His gca Ala tac Tyr 200 gaa Giu atg Met gcc Ala cgC Arg caa Gin 280 cac His cca Pro gac Asp 90 cgc Arg gc t Ala.

cgc Arg gtt Val1 ctc Leu 170 ttC Phe tcC Ser ttc Phe gca Ala caa Gin 250 ctg Leu gtC Val acc Thr gat Asp 75 ggt Gly tcC S er gat Asp ctc Leu gtc Val 155 cat His aag Lys gcc Ala t tc Phe gtc Val 235 gca Ala aaa Lys t tC Phe gtc Val 60 gt t Val ggC Gly ggc Gly gtC Val cac His 140 atc Ile gtc Val caa Gin tac Tyr ctc Leu 220 aac Asn gcc Ala ttc Phe gcc Ala 45 gac Asp gga Gly tgg Trp tac Tyr t tC Phe 125 att Ile gca Ala gtt Val acc Thr cta Leu 205 gga Gly atc Ile cgc Arg gcc Ala ggc Gly 285 gag Glu acc Thr gcg Ala caa Gin 110 acg Thr t tg Leu gta Val tgc Cys gac Asp 190 gcg Ala cgc Arg gtg Val gaa Giu ttc Phe 270 gaa Glu ct t Leu atc Ile ttc Phe 95 tac Tyr gta Val gaa Glu gtc Val gac Asp 175 gct Ala aaa Lys 8CC Thr gcc Ala atc Ile 255 aat Asn gcc Ala tac Tyr ctg Leu 80 tgc Cys gcC Ala gat Asp gtc Val aac Asn 160 ctc Leu gac Asp atg Met tac Tyr gac Asp 240 aaC Asn ctc Leu 8CC Thr gcc Ala 8CC Thr ggc Gly gaa Glu gcc Ala 130 cgc Arg tgg Trp atc Ile gga Gly gga Gly 210 gcC Ala ggC Gly 888 Lys gac Asp ctg Leu 290 Ctc Leu ggC Gly ggc Gly cac His 115 cgc Arg ctc Leu gca Ala gct Ala tcc Ser 195 cag Gin gaa Giu gac Asp tCC Ser gat Asp 275 gcc Ala gac Asp aac Asn gac Asp 100 gcg Ala 8cc Thr atc Ile gcc Ala tC Ser 180 ttC Phe 888 Lys cgC Arg c ta Leu ccc Pro 260 ggc Gly tac Tyr cac His ggc Gly caa Gin cgc Arg 888

LYS

cgc Arg ggc Gly 165 cgc Arg gac Asp aac Asn atg Met gaa Glu 245 8CC Thr ctc Leu atg Met 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 WO 01/00843 WO 0100843PCTI/iBOO/00923 acg gat gaa gcc gta gag ggt aag gaa gca ttc cta gaa aag cgc gaa 1027 Thr Asp Giu Ala Val Glu Giy Lys Glu Ala Phe Leu Giu Lys Arg Giu 295 300 305 ccc aac tgg aat gaa ttc cct tac tac tac tagtgagttc atggggtcct 1077.

Pro Asn Trp Asn Glu Phe Pro Tyr Tyr Tyr 310 315 aaa 1080 <210> 498 <211> 319 <212> PRT <213> Corynebacterium glutamicum <400> 498 Met Ser Asn Tyr Ser Thr Asp Asn Pro 1 Thr Val Pro Gin Leu Ser Thr Ile Gin 145 Gly Thr Val 5 Phe Arg Asn His Gly Gin Arg Lys Arg Gly 165 Arg Asp Giu Ala Ala Ala 70 Pro Arg Asp Val Thr 150 Gly Gin Ala Phe Gly Arg Arg Glu Arg Al a 120 Gly Pro His Ala Tyr 200 Phe 10 Asp Val His Pro Asp 90 Arg Ala Arg Val Leu 170 Phe Ser Gln His Phe Glu Thr Ala Gin 110 Thr Leu Val Cys Asp 190 Ala Trp Arg Asp Leu Ile Phe Tyr Val Giu Val Asp 175 Ala Lys Ala His Arg Tyr Leu Cys Ala Asp Val Asn 160 Leu Asp Met Val Gly Gin Lys Asn Ala Arg Glu Ile Phe Phe Leu Gly Arg Thr Tyr WO 01/00843 210 215 Asp Ala Giu Arg Met Gin Gin Met Gly Ala Val 225 230 235 His Gly Asp Leu Giu Lys Giu Ala Ile Gin Ala 245 250 Thr Lys Ser Pro Thr Gly Gin Arg Met Leu Lys 260 265 Thr Asp Asp Giy Leu Met Gly Gin Gin Val Phe 275 280 Arg Leu Ala Tyr Met Thr Asp Glu Ala Val Glu 290 295 Leu Glu Lys Arg Giu Pro Asn Trp Asn Giu Phe 305 310 315 <210> 499 <211> 384 <212> DNA <213> Corynebacterium giutamicum <220> <221> iCDS <222> (101)..(361) <223> RXS02908 <400> 499 gccaacgagg gttggtttac cacctctgat tcaggtgaac gtgactggtc gcgtggatac ccgtcattga ttccggtgga PCTIBOOIOO923 220 Asn Ala Phe Ala Gly 300 Pro gag gta ctg gaa cgt gcc atc gca gi Glu Val Leu Glu Arg Ala Ile Ala A! tgt gtt gtg ggt att ccc gat ccc c Cys Val Val Gly Ile Pro Asp Pro Ai 251 gcg tac tcc gga tcg atc agt ccg tc Ala Tyr Ser Gly Ser Ile Ser Pro SE 45 gat cta cct cgt tgg cag ctt ccc az Asp Leu Pro Arg Trp Gin Leu Pro L) 60 ttg ccc agc att ggt cct gga aaa g( Leu Pro Ser Ile Gly Pro Giy Lys A] 75 ctg ttt tagtcttcat tcttgctggc tgc Leu Phe aaa Lys ggc Gly gtt Val1 ctg Leu cga Arg tccacgacgg gattctcacc ttg aag ttg cac cca Leu Lys Leu His Pro 1 ggt gtc acc gcg gcg Gly Val Thr Ala Ala caa gca att gtg gcc Gin Ala Ilie Val Ala att gaa ggc ctc gac Ile Glu Gly Leu Asp aag cat ctg gaa tct Lys His Leu Glu Ser cg t Arg gct atc gcg Ala Ile Ala WO 01/00843 WO 0100843PCTIIBOO/00923 <210> 500 <211> 87 <212> PRT <213> Corynebacterium glutanicun <400> 500 Leu Lys Leu His Pro Giu Val Leu Giu Arg 1 5 10 Gly *Vai Thr Ala Ala Cys Val Val Gly Ile 25 Gin Ala Ilie Val Ala Ala Tyr Ser Gly Ser 40 Ilie Glu Giy Leu Asp Asp Leu Pro Arg Trp 55 Lys His Leu Giu Ser Leu Pro Ser Ile Gly 70 Arg Ala Ile Ala Lys Leu Phe Ala Pro Ilie Gin Pro 75 <210> 501 <211> 775 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(775) <223> RXS03003 <400> 501 tcgatgatct gggctcccca ggtggtgcgg ctaagcttgg caatgatcgc tgcgctgccg cctcaggcat aatctaacgc Ile Ala Asp Ile Lys Asp Pro Arg Leu Gly Ser Pro Ser Giu Val Leu Pro Lys Arg Leu Gly Lys Ala Asp Arg accacaagat tttgatcacc atg acc tct cgc acc Met Thr Ser Arg Thr 1 gat tcg ctc gct tcc Asp Ser Leu Ala Ser atc gtg aat ctt tct Ile Val Asn Leu Ser att cag atc gcg ttg Ile Gin Ile Ala Leu acc atc ggc acc ccg Thr Ile Gly Thr Pro cgg cgc tac aac atg gt t Val gcc Al a act Thr gca Ala cgc tc t Ser aaa Lys ccg Pro gcg Ala gca ttt Phe ccg Pro 30 gcg Ala tac Tyr gcg 722 tgg Trp ggg Gly agc Ser caa Gin gag WO 01/00843 Glu Leu Arg Ala Ala Ile 75 PCTJl[BOOIOO923 Arg Gly Ala Leu Glu Arg Arg Tyr aca aag ctt gtc gac gcc tcc ctc ctc Thr Lys Leu Val Asp Ala Ser Leu Leu gca att gcc ctt ctt cca ttc gcg ttg Ala Ilie Ala Leu Leu Pro Phe Ala Leu 105 110 atc cca gag att gcg tac cca acc tac Ile Pro Glu Ilie Ala Tyr Pro Thr Tyr 120 125 gga tgc acc gtg ttg cgt tct gat tcg Giy Cys Thr Val Leu. Arg Ser Asp Ser 135 140 atc ccg tcg atg atg ttt atc aac tca Ilie Pro Ser Met Met Phe Ile Asn Ser 150 155 gtt ctg ggc atc cca cac ttg cgc aag Val Leu Gly Ile Pro His Leu Arg Lys 170 aac aac gtg atc ctc gca gct gat gaa Asn Asn Val Ilie Leu Ala Ala Asp Glu 185 190 gac gat gaa aac cca ccg atc tca att Asp Asp Glu Asn Pro Pro Ile Ser Ile 200 205 ggc gac cac acc aac ttg atc gcc att Gly Asp His Thr Asn Leu Ile Ala Ile 215 220 <210> 502 <211> 225 <212> PRT <213> Corynebacterium glutainicum <400> 502 Met Thr Ser Arg Thr Pro Leu Val Ser 1 5 Asp Ser Leu Ala Ser Ala Lys Ala Lys 25 Ile Val Asn Leu Ser Val Gly Thr Pro 40 Ilie Gin Ilie Ala Leu Ala Giu Ala Ala 55 Thr Ilie Gly Thr Pro Glu Leu Arg Ala 70 gtg Val1 t cc Ser gc t Ala aag Lys 145 aac Asn aag Lys ttg Leu c ca Pro ctg Leu 225 Pro Asp Phe Pro Trp Ser His Pro Asp Gly Pro Val Ala Pro Ser Ser Gly Tyr Pro Gin Arg Gly Ala Leu Giu WO 01/00843 Arg Arg Tyr Asn Met Thr Lys Leu Val Asp Ala 90 Val Gly Thr Lys Glu Ala Ile Ala Leu Leu Pro 100 105 Ser Gly Thr Val Val Ile Pro Giu Ile Ala Tyr 115 120 Ala Val Val Ala Ala Gly Cys Thr Val Leu Arg 130 135 Lys Leu Gly Pro Gin Ilie Pro Ser met Met Phe 145 150 155 Asn Pro Thr Gly Lys Val Leu Gly Ile Pro Hius 165 170 Lys Trp Ala Gin Glu Asn Asn Val Ile Leu Ala 180 185 Leu Gly Leu Giy Trp Asp Asp Giu Asn Pro Pro 195 200 Pro Arg Val Cys Asp Gly Asp His Thr Asn Leu 210 215 Leu 225 <210> 503 <211> 390 <212> DNA <213> Corynebacteriun glutamicun <220> <221> CDS <222> (101)..(367) <223> RXS03026 <400> 503 gttggcggcg cagtatcgtg aggtgcggga cctcgagcgg cgaactagcc ccccaacaac aattagaaat ggaacctaaa Ser Phe Pro Ser 140 Ile Leu Ala Ile Ile 220 Leu Leu 110 Tyr Ser Ser Lys Giu 190 Ile Ile PCTIiBOO/00923 Pro Val Gly Ile Glu Val Leu Phe Pro Ser 160 Val Val 175 Cys Tyr Leu Asp His Ser ggaatcccaa actagcatcc atg cct gga aaa att C tc Leu gac Asp gct Ala c ac ctt Leu att Ilie gag Glu caa ctc Leu tac Tyr gc t Ala ggt ctg Leu ttg Leu ctt Leu 45 gcg aac Asn gaa Glu gag Glu ctg 724 Met Pro Gly Lys Ile 1 atg ctg ggc aaa cgc gag cct Met Leu Gly Lys Arg Giu Pro gac gtc gtc gcg ctg gca acc Asp Val Val Ala Leu Ala Thr gtt gag gcg ctg cag agc aat Val Glu Ala Leu Gin Ser Asri cac aac gct cgc ggg acc cac 115 163 211 259 307 WO 01/00843 PCTIIBOO/00923 Hius Gin Gly Glu Leu Ile Asp Ala Leu His Asn Ala Arg Gly Thr His 60 atc ggt tgc gtg att aac ccc 99c ggc ctg act aca ctt cgg tgg cgc 355 Ile Giy Cys Val Ile Asn Pro Gly Gly Leu Thr Thr Leu Arg Trp Arg 75 80 ttt tgg atg ctg tgaaggcgtc tgagcttcct acc 390 Phe Trp Met Leu <210> 504 <211> 89 <212> PRT <2i3> Corynebacterium giutamicun <400> 504 Met Pro Gly Lys Ile Leu Leu Leu Asn 1 5 Gly Lys Arg Giu Pro Asp Ile Tyr Gly 25 Vai Ala Leu Ala Thr Ala Giu Aia Ala 40 Ala Leu Gin Ser Asn His Gin Giy Giu 55~ Ala Arg Giy Thr His Ilie Gly Cys Vai 70 Thr Leu Arg Trp Arg Phe Trp Met Leu Giy 10 His Lys Leu Ile Pro Asn Leu Asn Met Asp Thr Leu Giu Asp His Giy Leu Giu Vai Ile Asp Ala Leu His Asn Pro Gly Gly Leu 505 <211> 62i <212> DNA <213> Corynebacteriuni giutanicum <2 <221> CDS <222> (101)..(598) <223> RXS03074 <400> 505 tttgtgggca atctggtttt ttcgtaattg tgtgggatga atctcttaaa aattcacatt tagcaggaca agcatactgt tttagttcta tgctgtgggc atg act caa agt gct 115 Met Thr Gin Ser Aia 1 cca gaa ttc att gcc acc gca gac ctc gta gac atc atc ggc gac aac 163 Pro Glu Phe Ile Ala Thr Ala Asp Leu Val Asp Ile Ile Giy Asp Asn 15 gcg caa tca tgc gac act cag ttt caa aac ctt gga ggt gcc aca gaa 211 Ala Gin Ser Cys Asp Thr Gin Phe Gin Asn Leu Gly Giy Ala Thr Glu 30 WO 01/00843 WO 0100843PCTIiBOO/00923 ttc cac gga ata ata acc acc gtg aaa tgc ttc caa gac aac gcc ctc 259 Phe His Gly Ile Ile Thr Thr Val Lys Cys Phe Gin Asp Asn Ala Leu 45 ctg aaa tcc atc ctg agc gag gat aat cct ggg gga gtg ctg gtt atc 307 Leu Lys Ser Ile Leu Ser Glu Asp Asn Pro Gly Gly Val Leu Val Ile 60 gat ggc gac gca tcc gtg cac acc gcg cta gtt ggc gac atc att gca 355 Asp Gly Asp Ala Ser Val His Thr Ala Leu Val Gly Asp Ile Ile Ala 75 80 gga ctt gga aaa gat cat ggt tgg tcc gga gta att gtc aac gga gca 403 Gly Leu Gly Lys Asp His Gly Trp Ser Gly Val Ile Val Asn Gly Ala 95 100 att cga gac tcc gca gtc atc ggc acc atg acc ttt ggt tgt aaa gcc 451 Ile Arg Asp Ser Ala Val Ile Giy Thr Met Thr Phe Gly Cys Lys Ala 105 110 115 ctt gga acc aac ccg cgg aaa tcc act aaa act ggt tcc ggc gaa cga 499 Leu Gly Thr Asn Pro Arg Lys Ser Thr Lys Thr Giy Ser Gly Giu Arg 120 125 130 gac gta gtg gta tcg att ggt ggc att gac ttc att cct ggt cat tac 547 Asp Vai Val Val Ser Ile Gly Giy Ile Asp Phe Ile Pro Giy His Tyr 135 140 145 gtc tac gcg gac tct gac gga att atc gtc acc gag gcg cca att aag 595 Vai Tyr Ala Asp Ser Asp Giy Ile Ile Val Thr Glu Ala Pro Ile Lys 150 155 160 165 cag taatttgttt tgacgacgca gta 621 Gin <210> 506 <211> 166 <212> PRT <213> Corynebacteriun giutanicun <400> 506 Met Thr Gin Ser Ala Pro Giu Phe Ile Ala Thr Ala Asp Leu Val Asp 1 5 10 Ile Ile Gly Asp Asn Ala Gin Ser Cys Asp Thr Gin Phe Gin Asn Leu 25 Gly Giy Ala Thr Giu Phe His Gly Ile Ilie Thr Thr Val Lys Cys Phe 40 Gin Asp Asn Ala Leu Leu Lys Ser Ile Leu Ser Giu Asp Asn Pro Giy 55 Giy Val Leu Val Ile Asp Giy Asp Ala Ser Val His Thr Ala Leu Val 70 75 Gly Asp Ile Ile Ala Gly Leu Giy Lys Asp His Giy Trp Ser Gly Vai 90 WO 01/00843 WO 0100843PCTIIBOO/00923 Ile Phe Gly Ile 145 Giu Asn Cys 115 Gly Gly Pro Ala Ala Arg Tyr Lys 165 Ile Arg Asp Ser Ala Val Ile Gly Thr Met 105 110 Leu Gly Thr Asn Pro Arg Lys Ser Thr Lys 120 125 Asp Val Val Val Ser Ile Gly Gly Ilie Asp 135 140 Val Tyr Ala Asp Ser Asp Gly Ilie Ile Val 150 155 Gin <210> 507 <211> 3075 <212> DNA <213> Corynebacterium glutanicun <22 0> <221> CDS <222> (101)..(3052) <223> RXC01434 <400> 507 ggtttcctgc gcaccgtgat gattggtgcg gcgctgtcgc cggccatcgc ttcggcgttc aacactgcca acacgctgcc caacctgatc actggaaatc gtg ttg ggt gcg gtg Val Leu Gly Ala Val c tg Leu gcc Ala acg Thr ctg Leu tcc Ser ctg Leu ccc Pro tcg Ser ggC Gly ctg Leu Cgg Arg gcc Ala gcc Ala 9c9 Ala ctg Leu ggt Gly ggt Gly atg Met ttt Phe c tg Leu t99 Trp 105 9c9 Ala c tc Leu atc Ile gtc Val1 at t Ile cgt Arg atc Ile gaa Giu acc Thr ggc Gly aac Asn ttc Phe gaa Glu acc Thr WO 01/00843 ctg ggc gtg Leu Gly Val 120 PCT/iBOO/00923 tac atg gtg ctg cct gcg cgt ttg Tyr met Val Leu Pro Ala Arg Leu cac ccg cat His Pro His 125 130 gtg ggc Val Gly 135 ctt ggt Leu Gly 150 gcg ggc Ala Gly caa ttc Gin Phe cag ttc Gin Phe gct gcg Ala Ala 215 tat ggc Tyr Gly 230 tcc cgc Ser Arg cag ttg Gin Leu ttc ttc Phe Phe ggc caa 1027 Gly Gin 295 ttc tct 1075 Phe Ser 310 cgt gtg 1123 Arg Val att Ile gtg Val1 att Ile ggt Gly ggt Gly 200 ccg Pro atc Ile aac Asn gg t Gly acc Thr 280 ttc Phe gct Ala tt t Phe ttt Phe gtt Val gat Asp ggc Gly 185 tac Tyr ttt Phe atc Ile gcg Ala tcc Ser 265 gcc Ala gat Asp t tc Phe tat Tyr ga t Asp gca Ala atg Met 170 atg Met atc Ile att Ile Gly gca Ala 250 aag Lys t tc Phe gcc Ala acg Thr gcg Ala 330 cag Gin 140 tgt Cys cc t Pro atg Met acc Thr cag Gin 220 acc Thr ggc Gly acc Thr gtc Val1 gcc Ala 300 att Ile gaa Glu atc Ile cta Leu ctg Leu gcg Ala act Thr 205 cag Gin t tg Leu ga t Asp t tc Phe cct Pro 285 gcc Ala cct Pro gag Glu ttc Phe atg Met gg t Gly 175 atc Ile att Ile tgg Trp acc Thr agg Arg 255 gca Ala gcc Ala atc Ile gc t Ala tgg Trp 335 ctc Leu att Ile 160 atc Ile gtg Val gcg Ala atg Met gcg Ala 240 gca Ala ctg Leu aat Asn ctt Leu t tg Leu 320 acc Thr gag Giu acc Thr cgt Arg t tg Leu 180 atc Ile gac Asp gtt Vai cga Arg gac Asp 260 gtg Val gcc Ala ctg Leu cat His atc Ile 340 cag Gin acc Thr cgc Arg 165 aag Lys tcc Ser gat Asp cc t Pro c tg Leu 245 ct t Leu gtg Val tac Tyr agc Ser ctg Leu 325 atc Ile 499 547 595 643 691 739 787 835 883 931 979 WO 01/00843 WO 0100843PCT/EBOO/00923 gcc ggc 1171 Ala Gly ctg tcg 1219 Leu Ser ggt ttc 1267 Gly Phe 375 aac aaa 1315 Asn Lys 390 tgg gcg 1363 Trp, Ala ggg tgg 1411 Gly Trp agc tcc 1459 Ser Ser atc ttc 1507 Ile Phe 455 caa aac 1555 Gin Asn 470 att cgc 1603 Ile Arg cag gat 1651 Gin Asp atc acc Ile Thr 345 agc tcc Ser Ser gcc acc aag gtc gtg Ala Thr Lys Val Val 350 ccg gag cgt gtg gtg Pro Giu Arg Val Vai 365 ctt tcc ctg ttg gca ccg ctg Leu Ser Leu Leu Ala Pro Leu 355 gtg ctt ctt ggt gcg gcc aac Val Leu Leu Gly Ala Ala Asn 370 atc ggc gcg tat ctg ttg cgc Ile Gly Ala Tyr Leu Leu Arg 360 agt ttc atc acc ggc Ser Phe Ilie Thr Gly 380 ctc ggc ctg ttg ggt Leu Gly Leu Leu Gly 395 ttg ggc tct gcg gcg Leu Gly Ser Ala Ala 410 ctg att caa gcc gtc Leu Ile Gin Ala Val gcg gtc Ala Val atg cgc tct Met Arg Ser gtt ggt gca Val Gly Ala 415 385 ttg gct aaa acc tcc ctg Leu Ala Lys Thr Ser Leu 400 405 gca gca gca tgg gcg ttg Ala Ala Ala Trp Ala Leu 420 gtg Val 425 gta ggc Val Gly tac ttg ttg aac Tyr Leu Leu Asn 445 ggc atc gtg ttg Gly Ile Val Leu ggc gat ttc ttg ctg ggc act cta Gly Asp Phe Leu Leu Gly Thr Leu 430 435 ctg gct gtg ttg ggt gtc ttc ttc Leu Ala Val Leu Gly Val Phe Phe 450 tca cgt tct ggt ttg ccg gag gtc Ser Arg Ser Giy Leu Pro Giu Val acc Thr 460 465 ttg ggc cag gca Leu Giy Gin Ala 475 ccg aat acc aag Pro Asn Thr Lys 490 ttc tcc acc cag Phe Ser Thr Gin ctg acc cgc atc cca Leu Thr Arg Ile Pro 480 atc tct ttg gat gtc Ile Ser Leu Asp Val 495 ctg gtc gcg cca agc Leu Val Ala Pro Ser ggt Gly agc cgg ttt Ser Arg Phe 485 ggc gaa gtc tcc gag Gly Giu Val Ser Glu 500 gag ttc gca gca acc Glu Phe Ala Ala Thr 505 ccg cca 510 ccg atg tcc gcc ggt cct gtg 1699 att gtc cgc Ile Val Arg 515 cct cgc ctg Pro Arg Leu Pro Val Pro 520 Pro Pro Met Ser Ala 525 Gly WO 01/00843 gtt ccc ggc 1747 Val Pro Gly 535 cac ggc ggc 1795 His Gly Gly 550 aCC ggc aag 1843 Thr Gly Lys PCTAOBOO/00923 gcc cca gtc ggc Ala Pro Val Gly 540 gtc caa ggc gcg Val Gin Gly Ala gac ggt cgt ttc cgt ctg ctc gcc gat Asp Gly Arg Phe Arg Leu Leu Ala Asp 545 c;gt ttc tgg cag gcc cgc gag atc gcc Arg Phe Trp Gin Ala Arg Glu Ile Ala 560 565 gaa gtc Giu Val 570 cca ctg Pro Leu ctg atc ttt gtg Leu Ile Phe Val 575 tcg gca gcc gca Ser Ala Ala Ala gat act tcc ggc aac gcc Asp Thr Ser Gly Asn Ala 580 gcg ggc atc gcc tac gag Ala Gly Ile Ala Tyr Glu 595 cca ttt 1891 Pro Phe gtg cag 1939 Val Gin gcc ccc 1987 Ala Pro 615 gcc gat 2035 Ala Asp 630 gcc gat 2083 Ala Asp atc ggc 2131 Ilie Gly tgc cga 2179 Cys Arg gcg att 2227 Ala Ile 695 tcg gcc 2275 Ser Ala 710 gcg Ala tct Ser 585 cgc cgc Arg Arg 590 acc aag aag ctg Thr Lys Lys Leu 605 gcc Ala agc ttg ggc agc ttg gcg gta Ser Leu Gly Ser Leu Ala Val 610 atc cac tcc gag Ile His Ser Glu 620 tgg gtg cct ggc Trp Val Pro Gly 635 ccc cgc gcc gcc Pro Arg Ala Ala tcc Ser gcg Ala gcg tac cgc aac ggt Ala Tyr Arg Asn Gly 625 agc ttg agc gcc gtc Ser Leu Ser Ala Val 640 ttc gcg ctc gcg gaa Phe Ala Leu Ala Giu tgc ctc att gtg Cys Leu Ile Val gcg gaa tcc ggt Ala Giu Ser Gly 645 cta act gaa 8CC Leu Thr Glu Thr 660 gag gcc Glu Ala 665 att cgt Ile Arg gag atg ggt atc Glu Met Gly Ile 670 aac 8CC gac ggc Asn Thr Asp Gly 655 atc Ile ccg gcc ggc ttg gac aac aag Pro Ala Gly Leu Asp Asn Lys 675 cat gcc gtc ctc gcc ttg ccg His Ala Val Leu Ala Leu Pro 690 cgc gac gcc aag tcc ctg gcc Arg Asp Ala Lys Ser Leu Ala

CCC

Pro 685 gat gcc tca gag Asp Ala Ser Giu ctc Leu 700 gcc gag atg Ctt Ala Glu Met Leu 715 atc gac Ile Asp 705 gcg 8CC ctC gct Ala Thr Leu Ala 720 ccc agc gac gtc Pro Ser Asp Val 725 WO 01/00843 WO 0100843PCT/EBOO/00923 aag gca 2323 Lys Ala gat tac 2371 Asp Tyr gag gaa 2419 Glu Glu gcg aca 2467 Ala Thr 775 gca gcc 2515 Ala Ala 790 ggt acc 2563 Gly Thr tcC ccg 2611 Ser Pro gtg ggg 2659 Val Gly gac ggt 2707 Asp Gly 855 acc gca 2755 Thr Ala 870 acg cct 2803 Thr Pro gac agc 2851 Asp Ser atg gtc act Met Val Thr 730 gca tca ctt Ala Ser Leu 745 gaa gcc cag ggg Glu Ala Gin Gly gcc atg gcg atg Ala Met Ala Met 750 ctt gtg gtg aag Leu Val Val Lys cta gct aca gaa gac aat ccc Leu Ala Thr Glu Asp Asn Pro 735 .740 cgc acc tgc gga ctg ttc acc Arg Thr Cys Gly Leu Phe Thr 755 aag gaa aag aca cca aag cct Lys Glu Lys Thr Pro Lys Pro 770 gac tac acc gtc aag ggc atg Asp Tyr Thr Val Lys Gly Met cca Pro 760 cgt Arg acc cac Thr His gat ggt ttc Asp Gly Phe 765 ggt gcc tcc Gly Ala Ser atc gcc gct gtg Ile Ala Ala Val 795 gcg ttc ctc acc Ala Phe Leu Thr 810 780 785 gtg atc atc ttg gtt tcc Val Ile Ile Leu Val Ser 800 ctg gtg gcc gcc Leu Val Ala Ala 805 agc ttc ttc ggc Ser Phe Phe Gly ttg gcc Leu Ala 825 cca ccg Pro Pro tct gtt gaa gcc acc Ser Val Glu Ala Thr 830 gtc tac ctg gat ctg Val Tyr Leu Asp Leu 845 aca gat gtc acc gac Thr Asp Val Thr Asp agc agc acc aac gaa caa Ser Ser Thr Asn Glu Gln 820 tct gca aca cca gaa. cct Ser Ala Thr Pro Glu Pro 835 caa gcc cgc acg tgg gat Gln Ala Arg Thr Trp Asp gat

ASP

gga Gly gtc acc gac Val Thr Asp 860 tgg acc tcc acc Trp Thr Ser Thr 875 gcc cgc ctc gac Ala Arg Leu Asp 890 aac gtg acc tcg Asn Val Thr Ser 905 ggc Gly cgc Arg 865 ggc gac ggc ctc cta Gly Asp Gly Leu Leu 880 850 ggc aac acc tcc Gly Asn Thr Ser gtt gac ctg tcc Val Asp Leu Ser 885 gtc atc ttg Val Ile Leu 895 acc gtg aag atc Thr Val Lys Ile 910 acc acc ggc acc ggc tcc Thr Thr Gly Thr Gly Ser 900 tac gca ttc aac gac gcc Tyr Ala Phe Asn Asp Ala 915 WO 01/00843 tca cca ca 2899 Ser Pro Hi 92 tcc ggc cg 2947 Ser Gly Ar 935 ggt cag gt 2995 Gly Gin Va 950 caa acc tc 3043 Gin Thr Se gtt ggt tg 3075 Val Gly Tr tcc ctg tcg gaa ggc atc gag Ser Leu Ser Giu Gly Ile Giu 925 agt ctc agc cac agc atc cgc Ser Leu Ser His Ser Ile Arg 940 gaa tcc gtg gtg att ctg gtc Giu Ser Val Val Ile Leu Val 955 gac acc aat cca cag atg cag Asp Thr Asn Pro Gin Met Gin 970 975 taaattacgc gtttgtgatt gac PCTIIBOO/00923 gtg gat tat Vai Asp Tyr aag ctt ccg Lys Leu Pro cgt tcc tca Arg Ser Ser 965 gta caa ctt Vai Gin Leu 980 508 <2ii> 984 <2i2> PRT <2i3> Corynebacterium giutamicum <400> 508 Vai Leu Giy Aia Vai Leu Thr Ser Leu Aia Leu Ile Vali Ile Arg Ile His Giy 145 Giu Thr Giy Asn Phe Giu Thr Pro 130 Val Giy Leu Arg Aia Aia Aia Vai 120 Ile Val Giy Giy Met Phe Leu Trp 105 Tyr Phe Vali Vali S er Giy Met Aia Phe Aila Met Asp Aila Ile Giy Vai Leu Tyr Met Pro Vali Pro Gin 155 Leu Arg Leu Giu Leu Leu Asn Aia Ile Ile WO 01/00843 WO 0100843PCT/iBOOIOO923 Pro Asp Tyr Ser Leu 225 Ile Val1 Ile Gly Gly 305 Val Pro Leu Leu Ala 385 Ala Ala Leu Leu Gly 465 Tyr Ala Val Ile 210 Leu Met Val Pro Leu 290 Trp Leu Thr Leu Gly 370 Tyr Lys Ala Leu Gly 450 Leu Leu Arg Ala 195 Ala Gin Pro Ser Ile 275 Phe Thr Leu Phe Ala 355 Ala Leu Thr Trp Gly 435 Val Pro Arg Leu 180 Ile Asp Val Arg Asp 260 Val1 Ala Leu His Ile 340 Pro Ala Leu Ser Ala 420 Thr Phe Glu Arg 165 Lys Ser Asp Pro Leu 245 Leu Val Tyr Ser Leu 325 Ile Leu Asn Arg Leu 405 Leu Leu Phe Val Asp Gly 185 Tyr Phe Ile Ala Ser 265 Ala Asp Phe Tyr Thr 345 Ser Phe Gly G ly Ile 425 Gly Thr G ly Met 170 Met Ile Ile Gly Ala 250 Lys Phe Ala Thr Ala 330 Ala Pro Ile Leu Ser 410 Gin Tyr Gly Gin Arg Ala Ile Tyr Val 235 Asp Leu Giy Asn Leu 315 Arg Thr Glu Thr Leu 395 Ala Ala Leu Ile Ala 475 Pro Met Thr Gin 220 Thr Gly Thr Val Ala 300 Ile Glu Lys Arg Gly 380 Gly Ala Val Leu Val 460 Leu Trp Ile 190 Arg His Leu Asp Ile 270 Ile Asn Tyr Val Val1 350 Val Val1 Arg Gly Gly 430 Leu Ser Arg Ile Val Ala Met Ala 240 Al a Leu Asn Leu Leu 320 Thr Ser Leu Gly Leu 400 Ala Phe Val1 Ser Pro 480 WO 01/00843 Gly Leu Ser Gly Glu Val Glu Phe Ala 515 Arg Gly Pro 530 Arg Leu Leu 545 Ala Arg Glu Thr Ser Gly Gly Ile Ala 595 Gly Ser Leu 610 Gly Cys Leu 625 Val Ala Giu Giu Leu Thr Gly Leu Asp 675 Val Leu Ala 690 Ala Lys Ser 705 Ala Pro Ser Thr Glu Asp Cys Gly Leu 755 Lys Thr Pro 770 Thr Val Lys 785 Ser Leu Val Ile Gln Pro Val His 550 Thr Pro Val1 Ala Ala 630 Ala Ile Cys Ala Ser 710 Lys Asp Glu Ala Ala 790 Gly Arg Asp Val Pro 535 Gly Gly Phe Gin Pro 615 Asp Asp Gly Arg Ile 695 Ala Ala Tyr Giu Thr 775 Ala Thr Pro Phe Pro 520 Gly Gly Lys Ala Arg 600 Asn Trp Pro Glu Ile 680 Leu Ala Met Ala Pro 760 Arg Ile Ala Asn Ser 505 Pro Ala Val Giu Pro 585 Arg Ile Val Arg Ala 665 Arg Pro Giu Val1 Ser 745 Thr Asp Ala Phe Thr 490 Thr Pro Pro Gin Val 570 Leu Thr His Pro Ala 650 His Ile Asp Met Thr 730 Leu His Gly Ala Leu Lys Gin Met Val1 Gly 555 Ala Ser Lys Ser Gly 635 Ala Giu Asn Ala Leu 715 Glu Ala Leu Phe Val 795 Thr Ile Leu Ser Gly 540 Ala Leu Ser Lys Glu 620 Ser Ala Met Thr Se r 700 Ile Al a Met Val1 Gly 780 Val1 Ser Leu Ala 510 Gly Gly Phe Phe Al a 590 Ala Tyr Leu Al a Ile 670 Giy Leu Ala Gly Met 750 Lys Ser Ile Phe PCTIBOOIOO923 Asp Vai 495 Pro Ser Ile Val Arg Phe Trp Gin 560 Val Asp 575 Ala Ala Ser Leu Arg Asn Ser Ala 640 Leu Ala 655 Pro Ala His Ala Arg Asp Thr Leu 720 Leu Ala 735 Arg Thr Lys Giu Asp Tyr Leu Val 800 Gly Ser WO 01/00843 WO 0100843PCTIBOOIOO923 805 810 815 Ser Thr Asn Glu Gin Ser Pro Leu Ala Ser Val Giu Ala Thr Thr Ser 820 825 830 Ala Thr Pro Glu Pro Val Gly Pro Pro Val Tyr Leu Asp Leu Asp Gin 835 840 845 Ala Arg Thr Trp, Asp Asp Gly Ala Gly Thr Asp Val Thr Asp Val Thr 850 855 860 Asp Gly Asn Thr Ser Thr Ala Trp Thr Ser Thr Gly Gly Asp Gly Leu 865 870 875 880 Leu Val Asp Leu Ser Thr Pro Ala Arg Leu Asp Arg Val Ile Leu Thr 885 890 895 Thr Gly Thr Gly Ser Asp Ser Asn Val Thr Ser Thr Val Lys Ilie Tyr 900 905 910 Ala Phe Asn Asp Ala Ser Pro His Ser Leu Ser Glu Gly Ile Giu Ile 915 920 925 Gly Thr Val Asp Tyr Ser Gly Arg Ser Leu Ser His Ser Ilie Arg Asp 930 935 940 Ser Ser Lys Leu Pro Gly Gin Val Glu Ser Val Val Ile Leu Val Asp 945 950 955 960 Giu Val Arg Ser Ser Gin Thr Ser Asp Thr Asn Pro Gin Met Gin Ile 965 970 975 Ala Giu Val Gin Leu Val Gly Trp 980 <210> 509 <211> 930 <212> DNA <213> Corynebacterium glutamicum <22 0> <221> CDS <222> (907) <223> RXC02080 <400> 509 cgtaaattcg aagcgagctt ctaattctag caagcttggt gatggagtat cctgccaaaa tttgtcctgt tgcttattgt gcaggaattc ggaggcggac atg tca atc gag tgg 115 Met Ser Ile Giu Trp 1 tta caa att gtt gaa tta gga gcg atc ttt ggt gca ggt ttc ctc gca 163 Leu Gin Ilie Val Giu Leu Gly Ala Ile Phe Gly Ala Gly Phe Leu Ala 15 gga agc atc aat gta att gtc gga gca gga aca tta gtg tcg ttt cct 211 Gly Ser Ile Asn Val Ile Val Gly Ala Gly Thr Leu Val Ser Phe Pro 30 WO 01/00843 att ctc gtg Ile Leu Val acc atc ggc Thr Ilie Gly cgt gaa cta Arg Giu Leu tca atc ctc Ser Ilie Leu gca gat gtt Ala Asp Vai ctg ttg gtt Leu Leu Val 120 act tca ggt Thr Ser Gly 135 cga acc acc Arg Thr Thr 150 tat gga ggg Tyr Gly Gly ctt ggc atc Leu Gly Ilie aac ctc aca Asn Leu Thr 200 atc atc tcc Ile Ile Ser 215 ctt ggc tca Leu Gly Ser 230 ctt cgc ccc Leu Arg Pro acg gtc atc Thr Val Ile ctg Leu gtt Val gcc Ala ggg Gly ac a Thr gca Ala atc Ile atc Ile ttc Phe 170 tca Ser gcg Ala gag Giu tta Leu gt t Val 250 ggc Giy cct Pro cat His 75 atc Ile gca Ala gg t Giy tc t Ser gtc Val 155 agc Ser acg Thr gca Ala ttg Leu gg t Gly 235 ttt Phe ctt Leu gga Giy 60 gta Val1 acc Thr gta Val1 cca Pro gct Ala 140 tca Ser gca Ala ctg Leu gtt Val atc Ile 220 gga Gly aga Arg cca ccg ttg Pro Pro Leu 45 agt att tcg Ser Ile Ser aaa acc atc Lys Thr Ilie ggc gcc tcg Gly Ala Ser 95 att ccc tgg Ile Pro Trp 110 tca att aag Ser Ile Lys 125 ggg ttt agg Gly Phe Arg gta tgt ggt Val Cys Gly gct caa ggc Ala Gin Gly 175 cag atg cag Gin Met Gin 190 aat ctc atc Asn Leu Ile 205 tcc tgg ccg Ser Trp Pro tac atc ggc Tyr Ile Gly gca ttt gtg Ala Phe Val 255 PCTIBOOIOO923 act gcc acc atc gcc aac 259 Thr Ala Thr Ile Ala Asn ggt gtg gtt gct tat aga 307 Gly Val Val Ala Tyr Arg aga ttt ctg ctg cca gca 355 Arg Phe Leu Leu Pro Ala 80 ctc ttg ctg cat ttc tcc 403 Leu Leu Leu His Phe Ser 100 ctg att gga ttc ggc acg 451 Leu Ile Gly Phe Gly Thr 115 aag cat gtt ggc gct cat 499 Lys His Val Gly Ala His 130 caa ttg cct ttc ccg agc 547 Gin Leu Pro Phe Pro Ser 145 gcc ctg ttg ctg ggc atg 595 Ala Leu Leu Leu Gly met 160 165 att ctt ctc atc gca ttg 643 Ile Leu Leu Ile Ala Leu 180 gaa ctc aac gcc atc aaa 691 Giu Leu Asn Ala Ile Lys 195 gca gcc agt gtt ttt ata 739 Ala Ala Ser Val Phe Ile 210 acc gtt gcc tta atc gcg 787 Thr Val Ala Leu Ile Ala 225 gga cgg tac gcc cgc cga 835 Gly Arg Tyr Ala Arg Arg 240 245 gtc atc gtc gga atc acc 883 Val Ile Val Gly Ile Thr 260 atg acg atc ggt Met Thr Ile Gly taatgcagca gactagtaac ccc WO 01/00843 <210> 510 <211> 269 <212> PRT <213> Corynebacterium glutamicum <400> 510 met Ser Ile Giu Trp Leu Gin Ile Val PCTIBOO/00923 1 Ala Leu Ala Val Phe Leu Ile His Leu 145 Leu Leu Leu Ala Vai 225 Arg Gly Val Thr Val Leu Leu Gly Val 130 Pro Leu Leu Asn Ser 210 Ala Tyr Phe Ser Ile Ala Leu His Phe 115 Gly Phe Leu Ile Ala 195 Val1 Leu Al a Giu 10 Val Leu Val1 Ala Gly Thr Ala Ile Ile Phe 170 Ser Ala Giu Leu Val 250 Leu Ile Gly Pro His 75 Ile Ala Gly Ser Val 155 Ser Thr Ala Leu Gly 235 Phe Gly Val Leu Gly Val Thr Val1 Pro Ala 140 Ser Ala Leu Vai Ile 220 Gly Arg Ala Gly Pro Ser Lys Gly Ile Ser 125 Gly Val Ala Gin Asn 205 Ser Tyr Ala Ile Ala Pro Ile Thr Ala Pro 110 Ile Phe Cys Gin Met 190 Leu Trp Ile Phe Phe Gly Leu Ser Ile S er Trp Lys Arg Gly Gly 175 Gin Ile Pro Gly Val 255 Gly Thr Thr Gly Arg Leu Leu Lys Gin Aia 160 Ile Glu Ala Thr Gly 240 Val Ile Val Gly Ile Thr Thr Val Ile Val Met Thr Ile Gly <210> 511 <211> 669 <212> DNA WO 01/00843 WO 0100843PCTIIBOOIOO923 <213> Corynebacteriun glutamicum <220> <221> CDS <222> (101)..(646) <223> RXC02789 <400> 511 ttcttccgta cggctatgct taaaagctaa atttgtcttt ttcttaacac taaacaatgg aaaggtaagc gggtttttct gtgccttgca acacattaat atg aag gtt tcc gcc ii! Met Lys Val Ser Ala gat aca ccc ggt Asp Thr Pro Gly gat gat cca ggc Asp Asp Pro Gly ggc cgg cgc ctt Gly Arg Arg Leu ggc tta Gly Leu gat gtc ggc Asp Val Gly aag ctt gcc Lys Leu Ala gtg cgc atc gga Val Arg Ile Gly gca gcc tct gac Ala Ala Ser Asp cgc gat gcc Arg Asp Ala gga ttc aaa Gly Phe Lys atg cct gtg gaa acc gtt ccg cgg gaa Met Pro Val Glu Thr Val Pro Arg Glu 45 act Thr ggg cca gac ctg gcc gat att gat cgg ttg gtc gcc atc gtt gag gaa Gly Pro Asp Leu Ala Asp Ile Asp Arg Leu Val Ala Ile Val Glu Glu tac Tyr aac gcc gtg gaa Asn Ala Val Glu gtc Val att gtt ggt cta Ile Val Gly Leu aca gat ctg cag Thr Asp Leu Gin aat ggc tcc gcc Asn Gly Ser Ala agt Ser gtg aag cat gca Val Lys His Ala gaa att gct ttc Glu Ile Ala Phe cgc gtc Arg Val 100 cgt cgg cgc Arg Arg Arg gac gaa cgc Asp Giu Arg 120 acc aat gct gga Thr Asn Ala Gly aac att ccg gta Asn Ile Pro Val cgg ctt ggc Arg Leu Gly 115 cgg gcc tca Arg Ala Ser ctc acc acc gtc gtg gcc acc caa gcc Leu Thr Thr Val Val Ala Thr Gln Ala ttg Leu 130 gga gtc Gly Val 135 agc gaa aaa gcg Ser Glu Lys Ala cgt aaa gtt att Arg Lys Val Ile caa gct gcc gca Gin Ala Ala Ala gta Val 150 gaa atc ctt caa acc tgg ttg gat gct Giu Ile Leu Gin Thr Trp Leu Asp Ala 155 acc cga gcc ctt Thr Arg Ala Leu cca caa tcc aca Pro Gln Ser Thr gac Asp 170 acc caa gat ttc Thr Gln Asp Phe gag aag gga aat Giu Lys Gly Asn ttc cca Phe Pro 180 tgaaccaaat ccgaaaccgc cgg WO 01/00843 WO 0100843PCT/iBOO/00923 <210> 512 <211> 182 <212> PRT <213> Corynebacteriui glutamicui <400> 512 Met Lys Val Ser Ala Asp Thr Pro Gly His Asp Asp Pro Gly Pro Gly 1 Arg Ser Giu Ala Thr Ie Pro Ala Asp 145 Thr Lys 5 Leu Ala Lys Glu Gly Val Gly Ser Al a Gi u 165 Pro Thr Val Met Pro Leu Ala Val Glu Ala Ser Leu Thr 105 Leu Thr Glu Lys Leu Glri Thr Asp 170 Ile Glu Ile Ile Lys Al a Val Gly 140 Trp, Gin Gly Thr Asp Val His Gly Val 125 Arg Leu Asp <210> 513 <211> 903 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(880) <223> RXC02295 <400> 513 ttcccatgct aatccgcgaa aatggcactt caacagccat atactttaaa caacacaaat gatgcaagac tgataccggg atgtgatagg agcgcaccac atg ggg ttg gaa tta 115 Met Gly Leu Glu Leu 1 WO 01/00843 gca gct agc ggg tgg ggc atc ctc atc gca ggc gcc gcc gta PCTJIBOO/00923 Ala Ala Ser Gly t99 Trp atc Ile aac Asri gtc Val1 gtg Val gac Asp ggC Gly gcc Ala ctc Leu 150 ctc Leu gca Ala att Ile ctg Leu ctt Leu 230 atc Ile ctc Leu aa a Lys agg Arg gca Ala aaa Lys ctg Leu ctg Leu 135 atc Ile atc Ile gcc Ala gta Val1 gca Ala 215 ggt Gly gac Asp gcg Ala ctg Leu cgc Arg gc t Ala caa Gin atc Ile 120 ccc Pro gca Ala atg Met atg Met ttc Phe 200 gtc Val1 ggC Gly gca Al a gtc Val1 gca Ala gtc Val gtg Val1 atc Ile 105 gtg Val acc Thr gcc Ala gcg Ala gcg Ala 185 atc Ile gcc Al a gg t Gly Trp gta Val a tg Met gcc Ala aaa Lys tgc Cys a tg Met gtg Val1 gga Gly tac Tyr ttc Phe 170 aag Lys atc Ile aat Asn acc Thr Gly Ile Leu Ile Ala Gly Ala Ala Val atc Ile ccg Pro gtc Val1 ccc Pro 75 tcc Ser cga Arg ttc Phe t99 Trp gac Asp 155 acc Thr gtc Val ggC Gly gtc Val agg Arg 235 ggt Gly ctc Leu 45 ggc Gly aaa Lys gca Ala ctg Leu cc a Pro 125 cgc Arg atc Ile ctg Leu a ac Asn c ac His 205 ggc Gly att Ile ggc Gly 30 gca Ala acg Thr aaa Lys ggc Giy atc Ile 110 aac Asn tgg Trp ttc Phe ctc Leu acc Thr 190 a tg Met gca Ala aga Arg 15 gga Gly cc t Pro gca Ala ctg Leu gcc Ala 95 atc Ile ttc Phe gcc Ala gga Gly tcc Ser 175 gca Al a tgg Trp caa Gin tac TPyr c tc Leu gtg Val1 tcg Ser ctt Leu 80 c tg Leu gtg Val1 gga Gly gcc Ala ccc Pro 160 caa Gln aca Thr tgg Trp ctc Leu gca Ala 240 gtc Val aca Thr 9c9 Ala 9c9 Ala gc t Ala ttg Leu acc Thr atc Ile 145 gga Gly aa t Asn aac Asn acc Thr gg t Gly 225 cta Leu gcc Ala ccg Pro gcc Ala acc Thr gtt Val1 Ctc Leu 100 gtc Val agc Ser gtc Val1 atg Met tcc Ser 180 9c9 Ala ctc Leu acg Thr ctg Leu gga Gly ctg Leu tcc Ser ctg Leu ctg Leu att Ile gtt Val1 aaa Lys gga G ly ttc Phe 165 tcc Ser cta Leu gtg Val gtg Val1 gtt Val1 245 163 211 259 307 355 403 451 499 547 595 643 691 739 787 835 WO 01/00843 PCTILBOO/00923 gtc gtc atg agc gtc tac Ctc acc tgg caa caa atc caa gga atg 880 Val Val Met Ser Vai Tyr Leu Thr Trp Gin Gin Ile Gin Gly Met 250 255 260 tagataagtc ggggcaaact cta 903 <210> 514 <211> 260 <212> PRT <213> Corynebacterium giutamicum <400> 514 Met Gly Leu Giu Leu Ala Ala Ser Gly Trp Gly Ilie Leu Ile Ala Gly 1 5 10 Ala Ala Val Ala Gly Trp Ile Asp Ala Val Ile Gly Gly Giy Gly Leu 25 Val Leu Ilie Pro Leu Ile Leu Ala Val Met Pro Gin Leu Ala Pro Val 40 Thr Ala Leu Ala Ser Asn Lys Leu Ala Ala Val Thr Gly Thr Ala Ser 55 Ala Ala Phe Thr Leu Val Arg Arg Val Lys Pro Asp Lys Lys Leu Leu 70 75 Ala Leu Tyr Val Leu Vai Aia Ala Val Cys Ser Gly Ala Gly Ala Leu 90 Ala Ala.Ser Leu Ilie Asp Lys Gin Ile Met Arg Pro Leu Ile Ile Val 100 105 110 Leu Met Leu Val Val Gly Leu Ilie Val Val Phe Lys Pro Asn Phe Giy 115 120 125 Thr Gly Giu Ser Lys Ala Leu Pro Thr Gly Trp Lys Arg Trp Ala Ala 130 135 140 Ile Val Ala Val Gly Leu Ile Ala Ala Tyr Asp Gly Ile Phe Gly Pro 145 150 155 160 Gly Thr Gly Met Phe Leu Ile Met Ala Phe Thr Ala Leu Leu Ser Gin 165 170 175 Asn Phe Leu Ser Ser Ala Ala Met Ala Lys Val Val Asn Thr Ala Thr 180 185 190 Asn Leu Gly Ala Leu Ilie Val Phe Ile Ile Gly Gly His Met Trp Trp 195 200 205 Thr Leu Gly Leu Val Leu Ala Val Ala Asn Val Ala Gly Ala Gin Leu 210 215 220 Gly Ala Arg Thr Val Leu Gly Gly Gly Thr Arg Leu Ile Arg Tyr Ala 225 230 235 240 Leu Leu Thr Leu Val Val Val Met Ser Val Tyr Leu Thr Trp Gin Gin 245 250 255 WO 01/00843 WO 0100843PCTIBOOIOO923 Ilie Gin Gly Met 260 <210> 515 <211> 1132 <212> DNA <213> Corynebacteriun glutamicun <220> <221> <222> <223>

CDS

(1132) RXN0 3063 <400> 515 tgtctacaac ttcgccctca tggacatatt aacttcgttg cctcggagag aatactcatc acctagacaa cagtttgtat ctcacctcac aggaggaacc gtg gaa gat Val Giu Asp 1 ctc tca Leu Ser ggg ccg Gly Pro tac cgc atc ccg Tyr Arg Ilie Pro tcg cgc acc gtg Ser Arg Thr Val gag cag gtg cca Glu Gin Val Pro aag tcg aaa Lys Ser Lys ctt gca ccg Leu Ala Pro gcg Ala ctg gat gag cgt Leu Asp Giu Arg cga caa gca gca gta gca cga gca Arg Gin Ala Ala Val Ala Arg Ala 30 gtg gtg gac gca gac ggt ggc atc Val Val Asp Ala Asp Gly Gly Ilie ggt ctg cct gga Gly Leu Pro Gly ttg gct Leu Ala gac gcg gac ggc Asp Ala ASP Gly aac cgt ttc atc gac ctg gcc tcc ggc atc Asn Arg Phe Ile Asp Leu Ala Ser Gly Ile 60 gcc Ala gtg acc acg gtc Val Thr Thr Val gga tcc aac gcg Gly Ser Asn Ala gcc Ala gtc gcg aaa gcc Val Ala Lys Ala ggc gcc gca gct Gly Ala Ala Ala cgc ttc acc cac Arg Phe Thr His tgc ttc atg gtc Cys, Phe Met Val tca cct Ser Pro 100 tat gaa act Tyr Glu Thr ggc gat cac Gly Asp His 120 tac Tyr 105 gtg gcc atg gcg Val Ala Met Ala gag Glu 110 aga ctc aac gcc Arg Leu Asn Ala ttg act cca Leu Thr Pro 115 gcc gaa gcc Ala Glu Ala gac aag aag agc Asp Lys Lys Ser gcg Ala 125 ctg ttt aac tct Leu Phe Asn Ser ggc Gly 130 gtg gaa Val Glu 135 aac gcc gtc aag Asn Ala Val Lys gca cgc gcc tac Ala Arg Ala Tyr acc Thr 145 ggc aag ggc gcg Gly Lys Gly Ala gtC Val 150 gtg gtg ttc gac Val Val Phe ASP gcg tac cac gga Ala Tyr His Gly cgg Arg 160 acc aac ctc acc Thr Asn Leu Thr WO 01/00843 WO 0100843PCT/EBOO/00923 gcg atg acc gcg Ala Met Thr Ala aag Lys 170 aac cgc cca tac Asn Arg Pro Tyr tcc gga ttc gga Ser Gly Phe Gly cca cta Pro Leu 180 gcc gca gac Ala Ala Asp ctg tcc ggc Leu Ser Gly 200 gtc Val 185 tac cgt gca cca Tyr Arg Ala Pro tct tac cca ctg Ser Tyr Pro Leu cgc gac gga Arg Asp Gly 195 atc gaa tcc Ile Glu Ser ccg gaa gcc gca Pro Glu Ala Ala cgc gcg atc tcc Arg Ala Ile Ser cag gtc Gln Val 215 gga gcc gaa aac Gly Ala Glu Asn gcc tgc gtg gtc Ala Cys Val Val att Ile 225 gaa ccg atc cag Glu Pro Ile Gln ggc Gly 230 gaa ggc gga ttc Glu Gly Gly Phe gtc ccc gca cca gga ttc ctc gca gcc Val Pro Ala Pro Gly Phe Leu Ala Ala 240 tcc acc tgg tgc Ser Thr Trp Cys gag aac gac gtg Glu Asn Asp Val ttc atc gcc gat Phe Ile Ala Asp gaa atc Glu Ile 260 caa tct ggc Gln Ser Gly ggt gtg atc Gly Val Ile 280 ttc Phe 265 ctg cgc acc ggc Leu Arg Thr Gly tgg ttc gcc agc Trp Phe Ala Ser gac gca gaa Asp Ala Glu 275 gcc ggc ggc Ala Gly Gly ccc gac gtc atc Pro Asp Val Ile acc gca aaa ggc Thr Ala Lys Gly atg cca cta tcc gca gtg acc ggc cgc gca gaa atc atg gac gca ccc 1027 Met Pro 295 ggc ccc 1075 Gly Pro 310 gcc gcg 1123 Ala Ala Leu Ser Ala Val Gly Arg Ala Glu Ile 305 Met Asp Ala Pro ggc gcg ctc ggc gga acc tac ggc gga aac ccc gtt gct tgc Gly Ala Leu Gly 31 5 Gly Thr Tyr Gly Gly 320 Asn Pro Val Ala Cys 325 gca ctt gca gcc att gaa gtg atg gaa caa gcc gac ctt aag Ala Leu Ala 330 Ala Ile Glu Val Glu Gln Ala Asp Leu Lys 340 acc cgc gcg 1132 Thr Arg Ala <210> 516 <211> 344 <212> PRT <213> Corynebacteriun glutamicum <400> 516 Val Glu Asp Leu Ser Tyr Arg Ile Pro Gin Ser Arg Thr Val Ala Glu WO 01/00843 WO 0100843PCT/IB00100923 Gin Ala Ala Leu Val1 Phe Asn Ser Thr 145 Thr Gly Pro Ser Ile 225 Phe Ile Ala Gly Ile 305 Asn Val Val1 Asp Ala Ala Met Ala Gly 130 Gly Asn Phe Leu Val1 210 Glu Leu Ala Ser Ile 290 Met Pro Lys Leu Leu Ala 70 Gly Tyr Gly Val Val 150 Ala Ala Leu Gin Gly 230 Ser Gin Giy Met Gly 310 Ala Ser Ala Ala 55 Val Ala Giu Asp Glu 135 Val1 Met Ala Ser Val 215 Glu Thr Ser Val Pro 295 Pro Ala Lys Pro Asp Thr Ala Thr His 120 Asn Val Thr Asp Gly 200 Gly Giy Trp Gly Ile 280 Leu Gly Ala Ala Gly Ala Thr Ala Tyr 105 Asp Ala Phe Ala Val 185 Pro Ala Gly Cys Phe 265 Pro Ser Ala Leu Leu Leu Asp Val Ala Val1 Lys Val Asp Lys 170 Tyr Glu Glu Phe Arg 250 Leu Asp Ala Leu Ala 330 Asp Pro Gly Gly 75 Arg Al a Lys Lys Asn 155 Asn Arg Ala Asn Ile 235 Glu Arg Val Val Gly 315 Ala Glu Gly Asn Gly Phe Met Ser Val 140 Ala Arg Ala Ala Leu 220 Val Asn Thr Ile Thr 300 Gly Ile Gin Val1 Ile Ala Thr Arg Phe Ala Gly Lys 175 Ser Ala Val Pro Val1 255 Trp Ala Ala Gly Met 335 Ala Asp Asp Ala Cys Leu Asn Tyr Arg 160 Ser Tyr Ile Val1 Gly 240 Phe Phe Lys Giu G ly 320 Glu WO 01/00843 WO 0100843PCT/EBOO/00923 Gin Ala Asp Leu Lys Thr Arg Ala 340 <210> 517 <211> 1491 <212> DNA <213> Corynebacterium <220> <221>. CDS <222> (101)..(1468) <223> RXN02970 <400> 517 aaccgacaaa acagccgttc giutanicun acgtgctaaa gcagctcggc ttgatctagg gtgaggtgag ttatttaaag acttcataat attttgggga gtgaactggt ttg gca ttg aag Leu Ala Leu Lys tac acc aac ttt gac ggt gaa ttc atc Tyr Thr Asn Phe Asp Gly Giu Phe Ile aaa gaa gag gaa aaa cgg gca ttc gac Lys Giu Giu Giu Lys Arg Ala Phe Asp 30 ttc gga tct gtg Phe Gly Ser Val caa gca Gin Ala aac gat cgc gcg Asn Asp Arg Ala cac gtt ttc His Val Phe tgg gca gct Trp Ala Ala cac tcc tgg His Ser Trp tcc gcg cag gac Ser Ala Gin Asp atc agc ccc aaa Ile Ser Pro Lys gcc gaa Ala Glu ggt tcc acg ctg Gly Ser Thr Leu gac ttc gac ggc Asp Phe Asp Gly aac Asn gcc ttc atc gac Ala Phe Ile Asp a tg Met ggt tcc caa ctt Gly Ser Gin Leu tcg gca aac tta Ser Ala Asn Leu cac aac aac cct His Asn Asn Pro tta gtt gag gcg Leu Val Giu Ala cag cgc caa gca Gin Arg Gin Ala cgg ttg acc aac Arg Leu Thr Asn atc aac Ile Asn 100 ccg gcc ttc Pro Ala Phe tcg atg gcc Ser Met Ala 120 ggc Giy 105 aat gat gtg cgc Asn Asp Val Arg gat gtt gct gca Asp Val Ala Ala aag atc gtg Lys Ile Val 115 aac ggc ggc Asn Gly Gly cgt ggc gaa ttc Arg Gly Giu Phe cac gtg ttt ttc His Val Phe Phe acc Thr 130 gcc gac Aia Asp 135 gcc atc gag cac Ala Ilie Giu His aaa att ctg tcc Lys Ile Leu Ser 155 tcc Ser 140 atc cgc atg gct Ile Arg Met Ala ctg cac acc gga Leu His Thr Gly cgc Arg 150 aac Asn gca tac cgc agc Ala Tyr Arg Ser cac ggc gca acc His Gly Ala Thr WO 01/00843 tcc gcg atg atg ctc acc ggc Ser Ala Met Met Leu Thr Gly 170 gaa cac cgc cgc ctg Giu His Arg Arg Leu 175 acc gac Thr Asp tca ttc Ser Phe cac ttg His Leu 215 atc gtc Ile Val 230 gca ggt Ala Gly ctc ttc Leu Phe ctg ttt Leu Phe acc ttc 1027 Thr Phe 295 gtg atg 1075 Val Met 310 ggc gga 1123 Gly Gly gca gcg 1171 Ala Ala cga ctt 1219 Arg Leu gaa aac 1267 Giu Asn 375 cc a Pro ttt Phe 200 gaa Giu ctg Leu tac Tyr atc Ile gc t Ala 280 gcc Al a ac c Thr ctc Leu ctg Leu ggc Gly 360 gta Val1 gat Asp 185 gcc Ala gat Asp gag Glu tta Leu gcc Ala 265 tac Tyr aag Lys caa Gin acc Thr gag Glu 345 gct Ala gcg 'Ala tac Tyr acc Thr atc Ile gtg Val 235 9gc Gly gaa Glu cat His gtt Val atc Ile 315 tcc Ser tac Tyr ctg Leu gc t Ala cac His caa Gin gcg Ala 220 gtg Val gtg Val1 gtc Val gct Ala aac Asn 300 cgc Arg gga Gly gcg Ala atc Ile gac Asp t tc Phe gaa Giu 205 ttt Phe gga Gly cgc Arg atg Met ggc Gly 285 gca Ala gat Asp cac His gaa Giu gaa Glu 365 gtg Val tgg Trp 190 gaa Glu gaa Giu tca Ser gaa Giu gtc Val1 270 gac Asp ggt Gly acc Thr cca Pro gga Gly 350 cc t Pro cgg Arg gca Ala gaa Giu ggt Gly tca Ser ct t Leu 255 gga Gly gat Asp t ac Tyr ttc Phe ct t Leu 335 gag Giu cgc Arg ggc Giy cca Pro tgc Cys gc t Ala gga Gly 240 tgc Cys ttc Phe ttc Phe gcc Ala gga Gly 320 gca Ala atc Ile ctt Leu atc Ile PCTIIBOO/00923 ggc aac ccc acc 643 Gly Asn Pro Thr 180 ctg cac cac tcc 691 Leu His His Ser 195 cgc gca ctc aag 739 Arg Ala Leu Lys 210 atg atc gca gcg 787 Met Ile Ala Ala atc ctg cca cca 835 Ile Leu Pro Pro 245 aag cac ggc atc 883 Lys His Gly Ile 260 cgc acc gga aaa 931 Arg Thr Giy Lys 275 cca gac atg atc 979 Pro Asp Met Ile 290 ctc ggt ggc atc Leu Gly Gly Ile gag gca tac tcc Giu Ala Tyr Ser 325 gca ccc gcc aag Ala Pro Ala Lys 340 cca cgc gta gct Pro Arg Val Ala 355 gaa cta gcg gaa Giu Leu Ala Giu 370 ttc ttc tgg gca Phe Phe Trp Ala WO 01/00843 WO 0100843PCT[IBOO/00923 gtg gag ttc aat gca gac gcc act gcc at 1315 Val Glu Phe Asn Ala Asp Ala Thr Ala Mi 390 395 ttc aag gaa cgc ggc gtg tgg ccg atg at 1363 Phe Lys Glu Arg Gly Val Trp Pro Met I 410 41 atc gcg ccg ccg ctg acc acc act gat gi 1411 Ile Ala Pro Pro Leu Thr Thr Thr Asp A., 425 430 gac gcg gtg gaa gct gca gcc caa gct gi 1459 Asp Ala Val Giu Ala Ala Ala Gin Ala Vi 440 445 gcg ttg ttc taagttttct agataacaag gcc 1491 Ala Leu Phe 455 <210> 518 <211> 456 <212> PRT <213> Corynebacterium glutamicui <400> 518 Leu Ala Leu Lys Gly Tyr Thr Asn Phe A.

1 5 Gly Ser Val Gin Ala Lys Giu Glu Giu L] 25 Arg Ala His Val Phe His Ser Trp Ser A: 40 Lys Val Trp Ala Ala Ala Giu Giy Ser TI Asn Ala Phe Ile Asp Met Gly Ser Gin LD His Asn Asn Pro Arg Leu Val Glu Ala I: Leu Thr Asn Ile Asn Pro Ala Phe Gly A! 100 105 Ala Ala Lys Ilie Val Ser Met Ala Arg G' 115 120 Phe Thr Asn Gly Gly Ala Asp Ala Ile G', 130 135 :g gct ?t Ala 400 le tcc Ser gaa Giu gcc ggt Ala Gly ggc aac Gly Asn ttg gta Leu Val gag ctg acc Glu Leu Thr 450 gct Ala cga Arg gca Ala 435 ttc Phe Ile Asp Ile Phe Asn Ala Ser 110 His Arg gca Ala ttc Phe 420 ctg Leu gc t Ala Glu Asn Ser Asp Leu Ala Asp Val1 Met Phe Phe Lys Asp Ala Gin Arg Ser 125 Ile Arg Leu His Thr Gly Arg Asn Lys Ile Leu Ser Ala Tyr Arg Ser Tyr WO 01/00843 145 His Gly A] Leu Gly A) Phe Leu H: Giu Arg A: 210 Gly Met I] 225 Ile Ile L Asn Lys H: Giy Arg TI Gin Pro A) 290 Pro Leu G~ 305 Ser Giu A: Vai Ala P) Ile Pro A) 3! Arg Giu L 370 Giy Phe P1 385 Ala Giy A: Giy Asn A) Leu Val A: 4: Leu Thr P1 450 <210> 519 La Ls La le Ala au Pro Ls Giy 260 ~r Gly sp Met Ly Gly la Tyr ro Ala 340 ~g Val 55 au Ala 'ie Trp la Ala rg Phe 420 la Leu 35 ,ie Ala Ala Asp Phe Leu 215 Val1 Gly Phe Phe Phe 295 Met Gly Ala Leu Asn 375 Glu Lys Ala Ala Leu 455 Met

ASP

185 Ala Asp Giu Leu Ala 265 Tyr

LYS

Gin Thr Giu 345 Ala Ala Asn Arg Pro 425 Glu Leu i7 0 Ile Thr Val1 Pro Asn 250 Asp Giu Gly Ser Tyr 330 Ile Giu Ile Ala Giy 410 Leu Ala 155 Thr Tyr Thr Ile Val 235 Gly Giu His Vali Ile 315 Ser Tyr Leu Ala Asp 395 Val Thr Ala Gly His Gin Ala 220 Val Val Vai Aia Asn 300 Arg Giy Ala Ile Asp 380 Ala Trp Thr Ala Giu Phe Giu 205 Phe Gly Arg Met Gly 285 Ala Asp His Giu Glu 365 Vai Thr Pro Thr Gin 445 PCTIBOO/00923 160 Arg Arg 175 Ala Pro Giu Cys Giy Ala Ser Giy 240 Leu Cys 255 Gly Phe Asp Phe Tyr Ala Phe Gly 320 Leu Ala 335 Giu Ile Arg Leu, Gly Ile Met Ala 400 Ile Ser 415 Asp Giu Val Giu WO 01/00843 <211> 1330 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1330) <223> FRXA01009 <400> 519 aaccgacaaa acagccgttc acgtgctaaa gcagctcggc PCTIIBOO/00923 ttgatctagg gtgaggtgag ttatttaaag acttcataat attttgggga gtgaactggt ttg gca Leu Ala tac Tyr aaa Lys cac His gcc Ala atg Met tta Leu ccg Pro tcg Ser gcc Ala cgc Arg 150 t cc Ser aac Asn gag Glu tgg Trp ggt Gly tcc Ser gag Giu ttc Phe gcc Ala 120 gcc Ala aaa Lys atg Met gac Asp aaa Lys gcg Ala acg Thr ctt Leu atc Ile aa t Asn ggc Gly gag Giu ctg Leu ctc Leu 170 ggt Gly cgg Arg cag Gin ctg Leu gtc Val 75.

cag Gin gat Asp gaa Glu cac His tcc Ser 155 acc Thr gaa Giu gca Ala gac Asp tac Tyr tcg Ser cgc Arg gtg Val t tc Phe t cc Ser 140 gca Ala ggc Gly ttc Phe ttc Phe aaa Lys 45 gac Asp gca Ala caa Gin cgc Arg tcc Ser 125 atc Ile tac Tyr gaa Glu atc Ile gac Asp atc Ile t tc Phe aac Asn gca Ala tct Ser 110 cac His cgc Arg cgc Arg cac His gaa Giu 15 aac Asn agc Ser gac

ASP

tta Leu gcc Ala 95 gat Asp gtg Val atg Met agc Ser cgc Arg 175 t tc Phe gat Asp ccc Pro 99c Gly ggc Gly 80 c9g Arg gt t Val1 ttt Phe gc t Ala tac Tyr 160 cgc Arg 1 gga Gly cgc Arg aaa Lys aac Asn cac His t tg Leu gc t Ala ttc Phe cgc Arg 145 cac His ctg Leu ttg aag ggt Leu Lys Gly gtg caa gca Val Gin Ala cac gtt ttc His Val Phe tgg gca gct Trp Ala Ala ttc atc gac Phe Ile Asp aac cct cga Asn Pro Arg aac atc aac Asn Ile Asn 100 aag atc gtg Lys Ile Val 115 aac ggc ggc Asn Gly Gly cac acc gga His Thr Gly gca acc gga Ala Thr Gly 165 aac ccc acc Asn Pro Thr 180 acc gac cca gat atc tac cac ttc tgg gca cca ttc ctg cac cac tcc WO 01/00843 PCTIBOO/00923 le.Tyr His Phe Trp Ala Pro Phe Leu His His Ser 190 195 Thr Asp tca ttc Ser Phe cac ttg His Leu 215 ate gtc Ile Val 230 gca ggt Ala Gly ctc tte Leu Phe ctg ttt Leu .Phe ace ttc 1027 Thr Phe 295 gtg atg 1075 Val Met 310 ggc gga 1123 Gly Gly gca gcg 1171 Ala Ala cga ctt 1219 Arg Leu gaa aac 1267 Giu Asn 375 gtg gag 1315 Val Giu 390 Pro Asp 185 ttt gcc Phe Ala 200 gaa gat Giu Asp ctg gag Leu Glu tac tta Tyr Leu ate gcc Ile Ala 265 gct tac Ala Tyr 280 gcc aag Ala Lys acc caa Thr Gin etc ace Leu Thr ctg gag Leu Giu 345 ggc get Gly Ala 360 gta gcg Val Ala ttc aat Phe Asn :ace r Thr -ate L Ile 5L gtg SVal 235 Sggc iGly gaa SGlu ;cat .x His gtt SVal 3L atc r- Ile 315 tee r, Ser ctac B Tyr A ctg .i Leu get SAla 3t gac a ASP .395 caa Gin geg Ala 220 gtg Val gtg Val gte Val1 gct Ala aac Asn 300 egc Arg gga Gly gcg Ala atc Ile gac Asp 380 gcc Ala gaa Giu gaa Glu tca Ser gaa Giu gte Val 270 gac Asp ggt Gly acc Thr cca Pro gga Gly 350 cet Pro cgg Arg gcc Ala gca Ala atc Ile c tg Leu cac His acc Thr 275 gac Asp ggt Gly gca Ala ccc Pro cgc Arg 355 cta Leu t tc Phe get ctc Leu gca Ala cca Pro ggc Gly 260 gga Gly atg met Gly tac Tyr gcc Ala 340 gta Val gcg Ala tgg Trp gca aag Lys gcg Ala cca Pro 245 ate Ile aaa Lys atc Ile atc Ile tcc Ser 325 aag Lys get Ala gaa Giu gca Ala gaa 739 787 835 883 931 979 Met Ala Ala Gly Ala Ala Glu WO 01/00843 WO 0100843PCTILBOO/00923 ttc aag gaa cgc ggc 1330 Phe Lys Glu Arg Gly 410 <210> 520 <211> 410 <212> PRT <213> Corynebacterium giutamicun <400> 520 Leu Ala Leu Lys Gly Tyr Thr Asn Phe Asp Gly Glu Phe Ile Glu Phe 1 5 10 Gly Ser Val Gin Ala Lys Giu Giu Giu Lys Arg Ala Phe Asp Asn Asp 25 Arg Ala His Val Phe His Ser Trp Ser Ala Gin Asp Lys Ile Ser Pro 40 Lys Val Trp Ala Ala Ala Glu Gly Ser Thr Leu Tyr Asp Phe Asp Gly 55 Asn Ala Phe Ilie Asp Met Gly Ser Gin Leu Vai Ser Ala Asn Leu Gly 70 75 His Asn Asn Pro Arg Leu Val Glu Ala Ile Gin Arg Gin Ala Ala Arg 90 Leu Thr Asn Ile Asn Pro Ala Phe Gly Asn Asp Vai Arg Ser Asp Vai 100 105 110 Ala Ala Lys Ile Val Ser Met Ala Arg Gly Giu Phe Ser His Val Phe 115 120 125 Phe Thr Asn Gly Gly Ala Asp Ala Ile Glu His Ser Ile Arg Met Ala 130 135 140 Arg Leu His Thr Gly Arg Asn Lys Ile Leu Ser Ala Tyr Arg Ser Tyr 145 150 155 160 His Gly Ala Thr Gly Ser Ala Met Met Leu Thr Gly Giu His Arg Arg 165 170 175 Leu Gly Asn Pro Thr Thr Asp Pro Asp Ile Tyr His Phe Trp Ala Pro 180 185 190 Phe Leu His His Ser Ser Phe Phe Ala Thr Thr Gin Glu Glu Giu Cys 195 200 205 Giu Arg Ala Leu Lys His Leu Giu Asp Val Ile Ala Phe Glu Gly Ala 210 215 220 Gly Met Ile Ala Ala Ile Val Leu Glu Pro Val Val Gly Ser Ser Gly 225 230 235 240 Ile Ilie Leu Pro Pro Ala Gly Tyr Leu Asn Gly Val Arg Glu Leu Cys 245 250 255 WO 01/00843 Asn Lys His Gly Arg Thr 275 Gi~n Pro Asp 290 Pro Leu Gly 305 Ser Glu Ala Val Ala Pro Ile Pro Arg 355 Arg Giu Leu 370 Gly Phe Phe 385 Ala Gly Ala Asp Giu Gly Ser Tyr 330 Ile Giu Ile Aia Giy 410 Giu His Vali Ile 315 Ser Tyr Leu Ala Asp 395 Val1 270 Asp Gly Thr Pro Gly 350 Pro Arg Ala PCT/iBOO/00923 Gly Phe Asp Phe Tyr Ala Phe Gly 320 Leu Ala 335 Giu Ile Arg Leu Gly Ile Met Ala 400 <210> 521 <211> 1998 <212> DNA <213> Corynebacterium giutanicum <22 0> <221> CDS <222> (1975) <223> RXA01551 <400> 521 cactacgtag gcgtacccca ccatcaccgc gtcg agggggtaac ttttacgcgc acgcgtgcaa cgcg aaaagc gccctctctt aacccccgca ctagtt ttg aag gca gtc ccc Leu Lys Ala Val Pro 1 cg ctg att gct gcg cga gca la Leu Ile Ala Ala Arg Ala cg aag gaa gag agg aat ggt la Lys Glu Giu Arg Asn Gly ac ccg aaa cat agc tac tcc is Pro Lys His Ser Tyr Ser cg gag acc gaa atc cgc ctc acg ggc Thr Gly ccg ttt Pro Phe gca atg Ala Met ccc ate gcc Ala gaa Giu acg Thr cgc cgg gct gag atc g Arg Ala Giu Ile A tta Leu aa t Asn ctc ccg Pro atc Ile gtc 752 aag gac ggt WO 01/00843 WO 0100843PCTABOO/00923 Pro Ile Arg Lys Asp Giy Giu Val Pro Giu Thr Giu Ile Arg Leu ga t Asp gac tcg cca agc Asp Ser Pro Ser ggC Gly 75 ccc aac gaa ccc Pro Asn Glu Pro cgc atc tac cgc Arg Ile Tyr Arg acc Thr cgt ggc cca gaa Arg Giy Pro Giu aac ccc aag cag Asn Pro Lys Gin ctt ccg cgg ctg Leu Pro Arg Leu cgc gag Arg Giu 100 tca tgg atc Ser Trp Ilie cgt ttg ctt Arg Leu Leu 120 gcc cgc ggc gac Aia Arg Gly Asp gcc acc tat cag Ala Thr Tyr Gin ggg cgc gag Giy Arg Giu 115 ggt caa gct Gly Gin Ala atc gac gac ggc Ile Asp Asp Giy tcg gca atg cgt Ser Ala Met Arg cga Arg 130 tcg gct Ser Aia 135 gag tgg aaa ggc Giu Trp Lys Giy aaa cca gct cct Lys Pro Aia Pro ttg Leu 145 aag gcg cta cct Lys Aia Leu Pro aaa aga gtc acc LYS Arg Val Thr caa Gin 155 atg gcc tat gca Met Ala Tyr Ala gct ggc gtg att Aia Gly Val Ile cgt gaa atg gag ttt gta gcg ctg cgc gaa cac gtt gat gcg gag ttt Arg Glu Met Giu Phe Vai Ala Leu Arg Giu His Val Asp Ala Giu Phe 170 175 180 gtg cgc tct Val Arg Ser aac cac ccc Asn His Pro 200 gtg gcg cgc ggt Vai Ala Arg Gly gcc att att ccc Ala Ile Ile Pro aac aac gtc Asn Asn Val 195 ttt ttg acc Phe Leu Thr gaa tct gaa ccg Glu Ser Giu Pro att att ggt cgc Ile Ile Gly Arg aaa Lys 210 aaa atc Lys Ilie 215 aac gcc aat att Asn Ala Asn Ile ggc Gly 220 aat tct gcg gtc Asn Ser Ala Val tct tca atc gag Ser Ser Ile Giu gag gtg tcc aag Giu Val Ser Lys ctg Leu 235 cag tgg gcc acg Gin Trp Ala Thr tgg ggt gcc gat Trp Gly Ala Asp gtg atg gat cta Val Met Asp Leu acc ggc gat gat Thr Gly Asp Asp cac acc acc cgc His Thr Thr Arg gaa tgg Glu Trp 260 att atc cgc Ilie Ilie Arg gcg ctg gaa Ala Leu Giu 280 tcc ccc gtt cct Ser Pro Val Pro ggc acc gtc ccg Gly Thr Val Pro atc tac caa Ilie Tyr Gin 275 tgg gaa gta Trp, Giu Val aaa gta aat ggc Lys Val Asn Gly gcc gca gac ctt Ala Ala Asp Leu ttc cgc gat acc atc att gag cag tgt gaa caa ggc gtg gac tat atg 1027 WO 01/00843 WO 0100843PCT/EBOO/00923 Phe Arg 295 acc atc 1075 Thr Ile 310 cgt gtc 1123 Arg Val tgt ctg 1171.

Cys Leu ctg tgc 1219 Leu Cys ggc cta 1267 Gly Leu 375 gcc gag 1315 Ala Giu 390 gat gta 1363 Asp Val atc cag 1411 Ile Gin ttt tac 1459 Phe Tyr cac atc 1507 His Ile 455 gcc atg 1555 Ala Met 470 cgt gac 1603 Arg Asp Asp Thr Ilie Ilie cac gcc ggc gtc His Ala Gly Val 315 acc ggc att gtc Thr Gly Ile Val 330 Glu 300 ctg Leu Gin Cys Giu Gin ctg gct tat atc Leu Ala Tyr Ilie 320 Val Asp Tyr Met ctg act acc cgt Leu Thr Thr Arg 325 tcc cgc ggc gga Ser Arg Gly Gly 335 tcc att atg gcc ggt tgg Ser Ile Met Ala Gly Trp 340 tac gag cat ttc gac gag Tyr Giu His Phe Asp Giu 355 gcg cat Ala His 345 gaa atc Glu Ile cac cgc gaa tca ttc His Arg Glu Ser Phe 350 ttt gca caa tat gac Phe Ala Gin Tyr Asp 365 gga tcg ctt gcc gat Gly Ser Leu Ala Asp ctc Leu gtc gca ttc tcc ctc ggt gat Val Ala Phe Ser Leu Gly Asp 370 gcc aac gac gcc gcg caa ttc Ala Asn Asp Ala Ala Gin Phe ccc Pro 380 385 ctg aaa acc att Leu Lys Thr Ile 395 caa gta atg gtc Gin VJal Met Val ggt gag ctc acc caa Gly Giu Leu Thr Gin 400 gaa gga cct gga cac Glu Gly Pro Gly His cgc gcc tgg gaa tac Arg Ala Trp Glu Tyr 405 gtg cca cta aac atg Val Pro Leu Asn Met 420 gaa aac Glu Asn 425 act ctt Thr Leu 410 aac gag ctg gaa caa Asn Glu Leu Glu Gin 430 gga cca cta gtt acc Gly Pro Leu Vai Thr 415 aag Lys tgg gca gcg gac gca cct Trp Ala Ala Asp Ala Pro 435 440 act Thr tct gcc att ggt Ser Ala Ile Gly 460 445 gca gct Ala Ala gac atc gct cca ggt tat gac Asp Ile Ala Pro Gly Tyr Asp 450 cac atc gcc atg ggt ggc acc His Ile Ala Met Gly Giy Thr 465 gaa cac ctt ggc ctg ccc aac Glu His Leu Gly Leu Pro Asn ctg tgt tat gtc acc ccg aaa Leu Cys Tyr Val Thr Pro Lys 475 gac gtc aaa acc ggc gta atc Asp Val Lys Thr Gly Val Ile 480 acc tac Thr Tyr 485 aag ctc gct gcc cac Lys Leu Ala Ala His WO 01/00843 WO 0100843PCT/IBOO/00923 gca gca 1651 Ala Ala gcc atg 1699 Ala Met ctc tcc 1747 Leu Ser 535 ccg gca 1795 Pro Ala 550 aag ttc 1843 Lys Phe gat caa 1891 Asp Gin gct gtt 1939 Ala Val gaa ttt 1985 Giu Phe 615 gat gtg Asp Val 505 agt aaa Ser Lys aag ggt cat ccc Lys Gly His Pro 510 cgt ttt gaa ttc Arci Phe Glu Phe 495 500 ggc gcg cgt gcc tgg gac gac Gly Ala Arg Ala Trp Asp Asp 515 cgt tgg aat gat cag ttt gcg Arg Trp Asn Asp Gin Phe Ala 530 gcg Ala 520 ctc Lau gac ccc gac Pro Asp 525 Asp act gca atc gct tat cac gac gaa acc ctg Thr Ala Ile Ala Tyr His Asp Giu Thr Leu 540 545 acc gca cac ttc tgt tca atg tgt ggc ccg Thr Ala His Phe Cys Ser Met Cys Gly Pro 560 565 gag cct gcg aaa Glu Pro Ala Lys 555 tgc tcc atg cga Cys Ser Met Arg 570 atc gcg gaa ttg Ile Ala Glu Leu att agc cag Ile Ser Gin ggg atg cct Gly Met Pro 590 gca cgg gag Ala Arg Giu gac att cgc gat atg ttt ggc Asp Ile Arg Asp Met Phe Giy 575 580 ggg gtt ggg gat tct tct agt Gly Val Gly Asp Ser Ser Ser 595 ggg atg gct gag aaa tcc cgg Gly Met Ala Giu Lys Ser Arg 610 tat cgg cgt tagacagagc gc t Ala 600 at t Ile agt ggg Ser Gly gct Ala 605 ggt ggt gcg gag Gly Gly Ala Glu gtt Val Tyr Arg Arg 625 620 gatcccagat agc 1998 <210> 522 <211> 625 <212> PRT <213> Corynebacteriun glutamicum <400> 522 Leu LyS Ala Val Pro Thr Gly Ala Arg 1 5 Ile Ala Ala Arg Ala Pro Phe Glu Pro 25 Giu Glu Arg Asn Gly Ala Met Thr Pro 40 Ala Arg Ala Giu Ile Ala Leu 10 Val Arg Leu Ala Pro Ala Lys Thr Gin Asn Giu Ile His Pro WO 01/00843 Lys His Ser Thr Giu Ilie Arg Ile Tyr Pro Arg Leu Tyr Gin Gly 115 Arg Arg Gly 130 Leu Lys Aia 145 Ala Giy Val Vai Asp Ala Ile Pro Asn 195 Arg Lys Phe 210 Thr Ser Ser 225 Trp Giy Ala Thr Thr Arg Vai Pro Ilie 275 Leu Asn Trp 290 Gly Val Asp 305 Pro Leu Thr Ile Met Ala Giu His Phe 355 Tyr Arg Arg Arg 100 Arg Gin Leu Ile Giu 180 Asn Leu Ile Asp Glu 260 Tyr Giu Tyr Thr Gly 340 Asp Ser Leu Thr Giu Giu Al a Pro Thr 165 Phe Val1 Thr Glu Thr 245 Trp Gin Val1 Met Arg 325 Trp Glu Pro Asp 70 Arg Ser Arg Ser Gly 150 Arg Val1 Asn Lys Giu 230 Val Ile Ala Phe Thr 310 Arg Cys Leu Ile 55 Asp Giy Trp Leu Aia 135 Lys Glu Arg His Ile 215 Giu Met Ile Leu Arg 295 Ile Val Leu Cys Arg Lys Ser Pro Pro Giu Ilie Thr 105 Leu Ile 120 Giu Trp, Arg Val Met Giu Ser Glu 185 Pro Glu 200 Asn Ala Val Ser Asp Leu Arg Asn 265 Giu Lys 280 Asp Thr His Ala Thr Gly Ala His 345 Giu Ile 360 Arg Pro Asp Ser Thr 90 Ala Asp Lys Thr Phe 170 Val1 Ser Asn Lys Ser 250 Ser Val Ile Gly Ile 330 His Phe Giy Giy Gly 75 Asn Arg Asp Gly Gin 155 Val1 Ala Glu Ile Leu 235 Thr Pro Asn Ile Val 315 Val1 Arg Ala Ser Leu Pro Pro Gly Gly Gin 140 Met Ala Arg Pro Gly 220 Gin Gly Val1 Gly Giu 300 Leu Ser Giu Gin Leu Giu Asn Lys Asp Arg 125 Lys Ala Leu Gly Met 205 Asn Trp Asp Pro Val1 285 Gin Leu Arg Ser Tyr 365 Ala Val Giu Gin Val 110 Ser Pro Tyr Arg Arg 190 Ile Ser Ala Asp Ile 270 Ala Cys Ala Gly Phe 350 Asp Asp PCTIIBOO/00923 Pro Giu Pro Phe Gly Leu Ala Thr Ala Met Ala Pro Arg 160 His Ile Gly Val1 Arg 240 His Thr Asp Gin Ile 320 Ser Tyr Ala Asn Phe Ser Leu Gly Asp Giy Leu WO 01/00843 370 Asp Ala Al 385 Arg Ala Tr Val Pro Le Ala Ala As 43 Ala Pro G1 450 PCTIIBOO/00923 a y Gin Giu Asn 420 Ala Tyr Phe Tyr 405 Met Pro Asp Ala 390 Asp Ile Phe His 375 Giu Val Gin Tyr Ile 455 4

I

Ala Met Gly Gly Thr Ala Met Leu C 465 470 Leu Gly Leu Pro Asn Arg Asp Asp 485 Lys Leu Ala Ala His Ala Ala Asp N 500 Arg Ala Trp Asp Asp Ala met Ser 1 515 520 Asn Asp Gin Phe Ala Leu Ser Leu 530 535 His Asp Giu Thr Leu Pro Ala Giu 545 550 Ser Met Cys Gly Pro Lys Phe Cys 565 Arg Asp Met Phe Gly Asp Gin Ile 1 580 Gly Asp Ser Ser Ser Ala Val Ala 595 600 Ala Giu Lys Ser Arg Giu Phe Ilie 610 615 Arg 625 <210> 523 <211> 1013 <212> DNA <213> Corynebacteriun giutanicuin <220> <221> CDS <222> .(990) <223> RXA01019 Jy5 al1 ~sn L25 jeu ;er ~ys tal al1 ~05 .ys Ls p ?ro ~er ~85 3er Thr Met 410 Asn Gly Ala Tyr Lys 490 Ala Ala Pro Ala Met 570 Giu Ser Gly 380 Gly Giu Leu Leu Gly 460 Thr Gly Gly Phe Thr 540 Thr Ile Gly Ala Ala 620 Leu Pro Gin 430 Thr Ala Lys Ile Pro 510 Phe Ile His Gin Pro 590 Giu Val Thr Gly 415 Lys Asp His Giu Thr 495 Gly Arg Ala Phe Asp 575 Gly G ly Tyr Gin 400 His Trp Ile Ile His 480 Tyr Ala Trp Tyr Cys 560 Ile Val Met Arg WO 01/00843 PCTlIBOO/00923 <400> 523 act ttg gcg cac tca ctt tca ttc ccg gat tcg ctt cgc gac ggg cca 48 Thr Leu Ala His Ser Leu Ser Phe Pro Asp Ser Leu Arg Asp Gly Pro 1 5 10 acc gtt gga gac ctc ggg gaa ttt gaa gtg att cgg gta atc acg gag 96 Thr Val Gly Asp Leu Gly Giu Phe Glu Val Ile Arg Val Ile Thr Glu 25 caa gct gga tca tct ctc aac ggc gat gac gca gct gtg ctt cgg cat 144 Gin Ala Gly Ser Ser Leu Asn Gly Asp Asp Ala Ala Val Leu Arg His 40 gca tca ccc aat tcc agg gct gtt gtc acc acc gac atg ttg gtt gcg 192 Ala Ser Pro Asn Ser Arg Ala Val Val Thr Thr Asp Met Leu Val Ala 55 ggt agg cat ttc caa ttg gat tgg tcc acc ccg gaa caa ata ggg cag 240 Gly Arg His Phe Gin Leu Asp Trp Ser Thr Pro Giu Gin Ile Gly Gin 70 75 aag gcg att gtg cag aac ttt gct gat att gag gcg atg ggt gca cgt 288 Lys Aia Ile Val Gin Asn Phe Ala Asp Ile Giu Ala Met Gly Aia Arg 90 cca gta gcc gca ttg ttg gcg att tcc gcc ccc aca cac acc ccc gtg 336 Pro Val Aia Ala Leu Leu Ala Ile Ser Ala Pro Thr His Thr Pro Val 100 105 110 gag ttt gtc cga ggc tta gcc cgt ggc atg aat caa cgc ttg gag gag 384 Glu Phe Val Arg Gly Leu Aia Arg Gly Met Asn Gin Arg Leu Glu Glu 115 120 125 tac tcc gcg gaa ctt gtt ggc gga gac atc acc agc ggg gac tcc ttg 432 Tyr Ser Ala Giu Leu Val Gly Gly Asp Ile Thr Ser Gly Asp Ser Leu 130 135 140 gtt att gct gta act gca att ggt caa ctg ggt gga tcc ttg cca gag 480 Val Ile Ala Val Thr Ala Ile Giy Gin Leu Gly Giy Ser Leu Pro Glu 145 150 155 160 ctg acg ttg gga cgt gcc cga cca gga cag acc ctg gtg gcc cac gga 528 Leu Thr Leu Gly Arg Ala Arg Pro Gly Gin Thr Leu Vai Aia His Gly 165 170 175 aag atc ggt tac tcc gca gca ggc ctt gct cta ctg cag cac ttt ggt 576 Lys Ile Giy Tyr Ser Ala Ala Gly Leu Aia Leu Leu Gin His Phe Gly 180 185 190 cca gac aac gtt cca gag cac ctt cgc ccc ttg gtg gat gca cac tgc 624 Pro Asp Asn Val Pro Giu His Leu Arg Pro Leu Val Asp Ala His Cys 195 200 205 gca cca gtt ctc acc cca ggc cga ggc atg gtg gca cgc gct gct gga 672 Ala Pro Val Leu Thr Pro Gly Arg Gly Met Val Ala Arg Ala Ala Gly 210 215 220 gcg acc gcc atg act gat aac tcg gac gga ctg att gtg gat ctt aac 720 Ala Thr Ala Met Thr Asp Asn Ser Asp Gly Leu Ile Val Asp Leu Asn 225 230 235 240 WO 01/00843 caa atg gcc atg Gin Met Ala Met atc agc ccc gat Ile Ser Pro Asp 260 gac gcc tgg cga Asp Ala Trp Arg 275 tct acg acg ttt Ser Thr Thr Phe 290 gtc acc aaa aca Val Thr Lys Thr 305 ccc gca ttt tcc 1010 Pro Ala Phe Ser aag Lys 245 gaa Giu tgg Trp ggc G ly cgc Arg gat Asp 325 tc t Ser ctc Leu atc Ilie gat Asp cac His 310 gga Giy ggt gtg cgc Gly Val Arg ct t Leu t ta Leu gc c Ala 295 gaa Giu tgg Trp atc Ile 250 gcc Ala ggg Gly gga Gly gtc Val ttc Phe 330 PCTLBOO/00923 gat gtg gat tcc tgt agc 768 Asp Vai Asp Ser Cys Ser 255 gct tcc gta ctc gga aca 816 Ala Ser Val Leu Giy Thr 270 gaa gac cac acc ctg ctc 864 Glu Asp His Thr Leu Leu 285 ttc cgc acc atc ggc caa 912 Phe Arg Thr Ile Giy Gin 300 acc gta gat aag aaa acc 960 Thr Val Asp Lys Lys Thr 315 320 taatgaccaa caccctatgg aa t 1013 <210> 524 <211> 330 <212> PRT <213> Corynebacterium giutarnicum <400> 524 Thr Leu Ala His Ser Leu Ser Phe I 1 5 Thr Val Gly Asp Leu Gly Giu Phe( Gin Ala Gly Ser Ser Leu Asn Gly 2 Ala Ser Pro Asn Ser Arg Ala Val 1 Gly Arg His Phe Gin Leu Asp Trp 70 Lys Ala Ilie Val Gin Asn Phe Ala 2 Pro Val Ala Ala Leu Leu Ala Ile 100 Giu Phe Val Arg Gly Leu Ala Arg 115 120 Tyr Ser Ala Giu Leu Val Gly Gly 2 130 135 Asp Ile Leu Leu Ile Gly Thr 110 Leu Asp G ly Thr Arg Val1 Gly Ala Pro Giu Ser Pro Giu His Ala Gin Arg Val Giu Leu WO 01/00843 WO 0100843PCT/IBOO/00923 Val Ilie Ala Val Thr Ala Ile Gly Gin Leu Gly Gly Ser Leu Pro Glu 145 150 155 160 Leu Thr Leu Gly Arg Ala Arg Pro Gly Gin Thr Leu Val Ala His Gly 165 170 175 Lys Ilie Gly Tyr Ser Ala Ala Gly Leu Ala Leu Leu Gin His Phe Gly 180 185 190 Pro Asp Asn Val Pro Glu His Leu Arg Pro Leu Val Asp Ala His Cys 195 200 205 Ala Pro Val Leu Thr Pro Gly Arg Gly Met Val Ala Arg Ala Ala Gly 210 215 220 Ala Thr Ala Met Thr Asp Asn Ser Asp Gly Leu Ilie Val Asp Leu Asn 225 230 235 240 Gin met Ala Met Lys Ser Gly Val Arg Ile Asp Val Asp Ser Cys Ser 245 250 255 Ilie Ser Pro Asp Giu Leu Leu Ser Giu Ala Ala Ser Val Leu Gly Thr 260 265 270 Asp Ala Trp, Arg Trp Ilie Leu Ser Gly Gly Glu Asp His Thr Leu Leu 275 280 285 Ser Thr Thr Phe Gly Asp Ala Pro Ser Giy Phe Arg Thr Ile Gly Gin 290 295 300 Val Thr Lys Thr Arg His Giu Asp Leu Val Thr Val Asp Lys Lys Thr 305 310 315 320 Pro Ala Phe Ser Asp Gly Trp Arg Ser Phe 325 330 <210> 525 <211> 706 <212> DNA <213> Corynebacterium giutanicum <220> <221> CDS <222> (101)..(706) <223> RXA01352 <400> 525 gtgcccaatg cattgggctg agattgcgcg ctgttgctgc gcgggaccgt tcgaacctgt ctggttaaca ccagcgaagg aagcgaggat tgattgtccc gtg ttt gaa aat cgt 115 Val Phe Glu Asn Arg 1 ttt gac ctg cgt tgt tat gtt gtg act ggt gcg ggc tcg gtg gat gag 163 Phe Asp Leu Arg Cys Tyr Val Val Thr Gly Ala Gly Ser Val Asp Glu 15 gtt gtg cac act gcg tct gct gcg gct cgt ggt ggc gcg ggt gtg gtg 211 Val Val His Thr Ala Ser Ala Ala Ala Arg Gly Gly Ala Gly Val Val WO 01/00843 WO 0100843PCTA030O/00923 cag Gin tca Ser ctt Leu ctc Leu gag Giu gga Gly gat Asp ggt Gly 150 tcc Ser cgc Arg ttt Phe gtg Val1 aag Lys atc Ile ccg Pro gc t Ala acc Thr tac Tyr 135 cgg Arg aag Lys gc t Ala tct Ser cgt tca aag Arg Ser Lys gtt gcg ctt Val Ala Leu gac gac cac Asp Asp His att cac ggt Ile His Gly cgt gag ttg Arg Giu Leu 105 cta gaa ctt Leu Giu Leu 120 atc ggt gct Ile Gly Ala cca ccg att Pro Pro Ile gtg ccg atc Val Pro Ile 170 ctc agc gca Leu Ser Ala 185 gaa tct gat Giu Ser Asp 200 cct att tcg cca gaa gcg atg agg gag Ser 45 gcg Ala gtt Val1 ctt Leu cc t Pro gcg Ala 125 ttt Phe gcg Ala att Ile Pro cgg Arg gct Ala 9gg Gly gag Giu 110 gcg Ala Cgg Arg ggt Gly gg t Gly Giu Ala Met tgc Cys tct Ser cag Gin 95 gcg Ala aat Asn aag Lys tat Tyr gat Asp 175 agc Ser tcc Ser 80 gat Asp atc Ile gag Glu act Thr ccc Pro 160 gtc Val cca Pro tta Leu gat Asp att Ile ctg Leu ccc Pro 145 cc t Pro acc Thr Arg Giu aca acg Thr Thr atg cgc Met Arg atg tcg met Ser ggg ttg Gly Leu 115 tcc gat Ser Asp 130 acc aag Thr Lys ttg gtg Leu Val cct gcc Pro Ala t tg Leu agg Arg gaa Giu gtg Val 100 act Thr gtg Val1 gat Asp gaa Glu gat Asp 180 gca Ala gtg Val gga Gly crtt Leu act Thr ttg Leu tca Ser ttg Leu 165 gtg Val1 259 307 355 403 451 499 547 595 643 691 acc ggt gtg gct Thr Gly Val Ala 190 ggc gtt gcc atg gtg c99 gct Gly Val Ala Met Val Arg Ala 195 <210> 526 <211> 202 <212> PRT <213> Corynebacterium giutamicum <400> 526 Val Phe Glu Asn Arg Phe Asp Leu Arg Cys Tyr Val Val Thr Gly Ala 1 5 10 Gly Ser Val Asp Giu Val Val His Thr Ala Ser Ala Ala Ala Arg Gly 25 Gly Ala Gly Val Val Gin Val Arg Ser Lys Pro Ile Ser Pro Giu Ala 40 WO 01/00843 WO 0100843PCT/1BOO/00923 Met Arg Glu Leu Ala Ser Lys Val Ala Leu Glu Val Ala Arg Cys Ser 55 Pro Thr Thr Arg Val Leu Ilie Asp Asp His Leu His Val Ala Ser Ser 70 75 Leu Met Arg Glu Gly Leu Pro Ile His Gly Val His Leu Gly Gin Asp 90 Asp Met Ser Val Leu Glu Ala Arg Glu Leu Leu Gly Pro Glu Ala Ile 100 105 110 Ile Gly Leu Thr Thr Gly Thr Leu Giu Leu Val Ala Ala Ala Asn Glu 115 120 125 Leu Ser Asp Val Leu Asp Tyr Ile Gly Ala Gly Pro Phe Arg Lys Thr 130 135 140 Pro Thr Lys Asp Ser Gly Arg Pro Pro Ile Gly Leu Ala Gly Tyr Pro 145 150 155 160 Pro Leu Val Glu Leu Ser Lys Val Pro Ile Val Ala Ilie Gly Asp Val 165 170 175 Thr Pro Ala Asp Val Arg Ala Leu Ser Ala Thr Gly Val Ala Gly Val 180 185 190 Ala Met Val Arg Ala Phe Ser Glu Ser Asp 195 200 <210> 527 <211> 944 <212> DNA <213> Corynebacterium glutamicum <22 0> <221> CDS <222> (921) <223> RXA01381 <400> 527 tcc gca ggc gtt gga acc atc acg gtc atc gat gac gac acc gtc gac 48 Ser Ala Gly Val Gly Thr Ile Thr Val Ilie Asp Asp Asp Thr Val Asp 1 5 10 att tcc aac att cac cgc caa atc ctc ttc ggc gca agc gat gtc ggt 96 Ilie Ser Asn Ilie His Arg Gin Ile Leu Phe Gly Ala Ser Asp Val Gly 25 cga ccc aag gtc gag gtt gcc gcc gag cgc ctc aaa gaa ctc caa cca 144 Arg Pro Lys Val Glu Val Ala Ala Glu Arg Leu Lys Glu Leu Gln Pro 40 gac atc acc gtc aac gcg ttg cac gaa cgg atc act cca gaa aac gcc 192 Asp le. Thr Val Asn Ala Leu His Glu Arg Ile Thr Pro Glu Asn Ala 55 tgc gag ctg ctc aat tcc gtg gac ctc gtc tta gac ggc tcc gat tct 240 Cys Glu Leu Leu Asn Ser Val Asp Leu Val Leu Asp Gly Ser Asp Ser WO 01/00843 ttc tcc aca Phe Ser Thr ccc ctc arc Pro Leu Ile ttc aac tct Phe Asn Ser 115 ccc gaa caa Pro Giu Gin 130 gtt ctt ggc Vai Leu Giy 145 gcc atc gga Aia Ile Gly ctc tcc tac Leu Ser Tyr gcc gac ccg Aia Asp Pro 195 gca gcg cgc Aia Aia Arg 210 tcc ctc acc Ser Leu Thr 225 gac ctc ccc Asp Leu Pro acc tcg gac Thr Ser Asp art rrg gtc Ile Leu Val 275 aac tac tcc aaa Lys tgg Trp, 100 ggc Gly ccc Pro gcc Ala ttt Phe gac Asp 180 gcg Ala acc Thrgcc Ala gag Glu agc Ser 260 rac Tyr cac gtg tct gat Vai Ser Asp gta ctg cgc Vai Leu Arg 105 cac cgc gga His Arg Gly 120 gat ttc gtc ASP Phe Val 135 gcc acc atc Ala Thr Ile gaa atc ggc Giu Ile Gly ccc gcc gcc Pro Aia Ala 185 ctg gtc acc Leu Vai Thr 200 act tcg ctt Thr Ser Leu 215 atc cga gag Ile Arg Giu.

acg tca ctg Thr Ser Leu tta gag gca Leu Giu Ala 265 tcg gga arc Ser Gly Ile 280 cac aaa ttt His Lys Phe 295 gcc Ala trr Phe gtc Val1 cc t Pro ggc Gly gac Asp 170 acg Thr cgc Arg atc Ile cca Pro aag Lys 250 ctg Leu cgc Arg gtg Val 75 gcc Ala cac His ggc Giy gac

ASP

gca Ala 155 gtc Val cgc Arg ctc Leu gac

ASP

cat His 235 ctc Leu tct Ser agt Ser aat Asn gaa Giu ggc Gly ctg Leu tgc Cys 140 crc Leu caa Gin agc Ser cgc Arg gcc Ala 220 gaa Giu ccc Pro gga Gly tcc Ser ctt Leu PCTIIBOO/00923 gga act 288 Gly Thr gca crc 336 Ala Leu gtc ttc 384 Val Phe gct ggt 432 Ala Gly act cac 480 Thr His 160 aca atc 528 Thr Ile 175 gtc tcc 576 Val Ser rac gag 624 Tyr Giu aac ggc 672 Asn Gly crc aaa 720 Leu Lys 240 cag arc 768 Gin Ile 255 ggc gac 816 Gly Asp arc gac 864 Ile Asp ggg gtc 912 Gly Val Asn Tyr Ser His Leu 290 aac gcg ctg Asn Ala Leu 305 tagctgtcaa trraagaggc cag WO 01/00843 WO 0100843PCT/EBOO/00923 <210> 528 <211> 307 <212> PRT <213> Corynebacterium glutamicum <400> 528 Ser Ala Gly Val Gly Thr Ile Thr Val Ile Asp Asp Asp Thr Vai Asp 1 5 10 Ile Ser Asn Ile His Arg Gin Ilie Leu Phe Gly Ala Ser Asp Val Gly 25 Arg Pro Lys Val Giu Val Ala Ala Giu Arg Leu Lys Giu Leu Gin Pro 40 Asp Ile Thr Val Asn Ala Leu His Glu Arg Ile Thr Pro Giu Asn Ala 55 Cys Giu Leu Leu Asn Ser Val Asp Leu Val Leu Asp Giy Ser Asp Ser 70 75 Phe Ser Thr Lys Tyr Leu Val Ser Asp Ala Ala Giu Ile Thr Gly Thr 90 Pro Leu Ile Trp Ala Thr Val Leu Arg Phe His Gly Giu Leu Ala Leu 100 105 110 Phe Asn Ser Gly Pro Asp His Arg Gly Vai Giy Leu Arg Asp Vai Phe 115 120 125* Pro Glu Gin Pro Ser Ala Asp Phe Vai Pro Asp Cys Aia Thr Ala Gly 135 140.

Vai Leu Gly Aia Thr Thr Ala Thr Ile Giy Ala Leu Met Aia Thr His 145 150 155 160 Ala Ilie Giy Phe Leu Thr Giu Ile Gly Asp Vai Gin Pro Gly Thr Ile 165 170 175 Leu Ser Tyr Asp Aia Phe Pro Aia Aia Thr Arg Ser Phe Arg Vai Ser 180 185 190 Ala Asp Pro Ala Arg Pro Leu Vai Thr Arg Leu Arg Ala Ser Tyr Glu 195 200 205 Ala Aia Arg Thr Asp Thr Thr Ser Leu Ile Asp Ala Thr Leu Asn Giy 210 215 220 Ser Leu Thr Ala Leu Asp Ile Arg Glu Pro His Glu Val Leu Leu Lys 225 230 235 240 Asp Leu Pro Glu Gly Aia Thr Ser Leu Lys Leu Pro Leu Ser Gln Ile 245 250 255 Thr Ser Asp Ser Asp Ile Leu Giu Ala Leu Ser Giy Ile Asp Gly Asp 260 265 270 Ile Leu Val Tyr Cys Aia Ser Gly Ile Arg Ser Ser Asp Phe Ile Asp 275 280 285 WO 01/00843 WO 0100843PCTAIBOO/00923 Asn Tyr Ser His Leu Gly His Lys Phe Val Asn Leu Pro Gly Gly Val 290 295 300 Asn Ala Leu 305 <210> <211> <212> <213> <220> <221> <222> <223> 529 259

DNA

Corynebacteriun giutamicum

CDS

(101) (259) RXA01360 <400> 529 gtggcaatca acgccgcggt cgtacccaga tcccagtggt cacgcgccat ttgtgacaac gattccgtag aagttctcac cgcaattcag ggaggttaaa gat aaa act ttc gat tcc cac ctc atc atg ggc Asp Lys Thr Phe Asp Ser His Leu Ile Met Gly 15 atg ctg cat att Met Leu His Ile acc ggc gga Thr Giy Gly gcc acc Ala Thr tct cag gcg Ser Gin Ala ttg ctg gag Leu Leu Giu gaa tcc ctt gtc gcc agt gga Glu Ser Leu Val Ala Ser Gly 30 act caa ttg Thr Gin Leu acc acc gtg gcg atg cgt cga Thr Thr Val Ala Met Arg Arg cac caa His Gin gca acc acc Ala Thr Thr agc gga gaa 259 Ser Gly Giu <210> 530 <211> 53 <212> PRT <213> Corynebacteriun giutamicum <400> 530 Met Leu His Ile Ala 1 5 Asp Lys Thr Phe Asp Ser His Leu Ile 10 Met Giy Thr Gly Gly Ser Gly Thr Aia Thr Ser Gin Ala Leu Leu Giu Giu Ser 25 Gin Leu Thr Thr Val Ala Met Arg Arg His 40 Leu Vai Ala Gin Ala Thr Thr Ser Ser Gly Giu <210> 531 <211> 629 <212> DNA <213> Corynebacterium giutamicun WO 01/00843 WO 0100843PCTIBOOIOO923 <220> <221> CDS <222> <223> RXA01361 <400> 531 gcc gac g Ala Asp A 1 ct gtg atc la Val Ile 5 tct att gat ggc Ser Ile Asp Gly gat ccg tgt ttg Asp Pro Cys Leu acc gtg Thr Val acg atg aat Thr Met Asn gcg gcg ggc Ala Ala Gly ggg gtg agg gtt Gly Val Arg Val tcg aaa agc gtt Ser Lys Ser Val gtt gtt ttg Val Val Leu ctg ggc gcc gca Leu Gly Ala Ala att ccc ggc tgg ttt gag ggc gcg Ile Pro Gly Trp Phe Glu Gly Ala aac cca Asn Pro ttg cag ttg agg Leu Gin Leu Arg ccg Pro 55 gtg tac ggc gat Val Tyr Gly Asp at t Ile gtg cgc gtg cgc Vai Arg Val Arg ccg gag cga ctg Pro Giu Arg Leu ccg atg gtc acc Pro Met Val Thr gtg gtg cgc ggg Vai Val Arg Gly gtg gaa gat cgt Vai Glu Asp Arg cag Gin att tat atc att Ile Tyr Ile Ile cgt acc gat ggc Arg Thr Asp Gly acc ctc Thr Leu gcg atc ggc Ala Ilie Giy gca gtg cat Ala Val His 115 gcg Ala 100 aca agc cgt gag Thr Ser Arg Giu cac ccg caa cct His Pro Gin Pro cga acg ggc Arg Thr Giy 110 ccg ggc att Pro Gly Ile gat ttg cta cgc Asp Leu Leu Arg gat Asp 120 gct atc cgt ttg Ala Ile Arg Leu att Ile 125 gaa gaa Glu Giu 130 acc gaa ttt atc Thr Giu Phe Ile gaa Giu 135 gtc acc tgc ggc Val Thr Cys Giy gcc cgc ccc ggc acc Ala Arg Pro Gly Thr 140 tcc aat gtg att gcg Ser Asn Val Ile Ala gat gac ctg ccg Asp Asp Leu Pro ctg gga tgg gtt Leu Giy Trp Val tcc aca gga tat Ser Thr Gly Tyr ttC Phe 165 cgc cat gga att Arg His Gly Ile ctg tca gcc ctt Leu Ser Ala Leu ggt gca Giy Ala 175 cgc gct gcc Arg Ala Ala gat gtg tgc Asp Val Cys 195 gat atg gca acc Asp Met Ala Thr cag cca ctg ttc Gin Pro Leu Phe ccc act ctt Pro Thr Leu 190 gat ccg ttt cgc Asp Pro Phe Arg cac caa att His Gin Ile 200 taaggatttt tcacaagtga WO 01/00843 WO 0100843PCTIIBOO/00923 <210> 532 <211> 202 <212> PRT <213> Corynebacterium glutamicum <400> 532 Ala Asp Ala Val Ilie Ser Ile Asp Gly 1 Thr Ala Asn Val1 Val Ala Ala Glu Pro 145 Ser Arg Asp Met Ala Pro Pro Glu Ile Val Giu 130 Asp Thr Ala Val 5 Gly Gly Leu Leu Gin Thr Leu Phe Pro Phe 165 Asp Pro Vai Ala Arg Gin 70 Ile Ser Leu Ile Tyr 150 Arg Met Phe, Arg Al a Pro Pro Tyr Arg Arg Glu 135 Leu His Ala Arg Vai Ser Vai Met Ile Glu Asp 120 Val Gly Gly Thr His 200 Ala Ile Tyr Val1 Ile Asp 105 Ala Thr Trp Ile Asn 185 Gin His 10 Ser Pro Gly Thr Pro 90 His Ile Cys Val1 Leu 170 Gln Ile Leu Val Giu Arg Arg Giy Arg 110 Pro Pro Val Leu Pro 190 Thr Val1 Gly Val1 Gly Thr Thr Gly Gly Ile Gly 175 Thr Vai Leu Ala Arg Phe Leu Giy Ile Thr Ala 160 Ala Leu <210> 533 <211> 927 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (101)..(904) <223> RXA01208 <400> 533 tactaggcac ggggtgccaa ccggatggaa aaattccgga ggctgagaaa acacccgttg aacctgctct agctcgtact agcgaaggga tggccttaac gtg gct aac tcg ttt 115 767 WO 01/00843 t tg Leu t tg Leu gcg Ala t tc Phe gcg Ala gc t Ala gag Glu at t Ilie ggt Giy gcg Ala 150 ggt Gly t cg Ser ggc Gly tcc Ser ggC ga t Asp acc Thr ggt Gly gcc Ala gag Giu Gly agg Arg cgt Arg ggg Gly 135 gcg Ala gcg Ala ggg Gly gcg Ala gcg Ala 215 cag tct Ser aac Asn gcg Ala gct Ala caa Gin aca Thr acc Thr gga Gly 120 cgc Arg caa Gin gag Giu ga t Asp ctg Leu 200 ca c His att gtt Val atg Met a tg Met gga Gly atg Met t ta Leu gag Giu gag Giu gcg Ala 140 aag Lys gtg Val cag Gin tat Tyr t tg Leu 220 aag cga Arg caa Gin gtg Val1 45 gtg Val1 acc Thr gac Asp gga Gly gtc Val1 125 ac c Thr cgc Arg tct Ser c tg Leu cta Leu 205 gc t Ala gca caa Gin t tc Phe gat Asp C tc Leu aag Lys cca Pro atc Ile 110 gtg Vai ga t Asp act Thr gcg Aia gtg Val1 190 ggC Gly gcg Ala tcg aac Asn acg Thr act Thr atc Ile gcc Ala gtt Val1 gtc Val gcg Al a t CC Ser ggt Gly ga t Asp 175 att Ile gcc Ala cat His gcg Ala ctt Leu gtg Val gaa Glu gga Gly ggt Gly ggt Gly cag Gin ggg Gly 130 gtg Val gtg Val acg Thr gga Gly gac Asp 210 gtg Val agc Asn gt t Val ttg Leu tcg Ser act Thr gca Al a ggg Gly cct Pro 115 ctc Leu gcg Ala gc t Ala tgg Trp, tgc Cys 195 tca Ser gg t Gly ttt PCT/iBOO/00923 Ser Phe cag tgt 163 Gin Cys ctt gcc 211 Leu Ala gca gaa 259 Ala Giu cct tct 307 Pro Ser cga aaa 355 Arg Lys ttg tcg 403 Leu Ser 100 gcc gca 451 Ala Ala ggt gcc 499 Gly Ala ttg gag 547 Leu Giu gtc tct 595 Val Ser 165 ttg cgt 643 Leu Arg 180 tct ttg 691 Ser Leu gat att 739 Asp Ile gct gct 787 Ala Ala gcg gtg 835 Ala Val Gly Gin Ilie Ala Ala Gin Lys Ala Ser Ala Pro Gly Ser Phe WO 01/00843 230 gcg ttt att Ala Phe Ilie ttg gtt gat Leu Val Asp gat Asp gtg Val 265 PCTIBOOIOO923 240 245 tat gac gtg gat gcc cag gct gtg gcc tcg 883 Tyr Asp Val Asp Ala Gin Ala Val Ala Ser 255 260 gcc tgaaaagtac gtgactgatt ttt 927 Ala <210> 534 <211> 268 <212> PRT <213> Corynebacterium glutamicun <400> 534 Val Ala Asn Ser Phe Leu Asp Ser Leu 1 Pro Asn Ala Ala Glu Val Lys Ala Giu 145 Val Val Thr Val His 225 Val1 Leu Ser Thr Ala Gly Pro 115 Leu Al a Ala Trp Cys 195 Ser Gly Gin Leu Ala Pro Arg Leu 100 Ala Gly Leu Val Leu 180 Ser Asp Ala 5 Cys Ala Glu Ser Lys Ser Ala Ala Glu Ser 165 Arg Leu Ile Ala Leu Ala Phe Ala 70 Ala Glu Ile Gly Ala 150 Gly Ser Gly Ser Gly Thr Gly Ala Giu Gly Arg A rg Gly 135 Ala Ala Gly Ala Ala 215 Gin Thr 10 Val1 Pro Ala Gin Trp, 90 Tyr Ala Val1 Leu Leu 170 Met Ala Ala Ala Leu Val Ala Asn Gly 75 Val1 Ala Ser Asp Al a 155 Ile Leu Ala Val Gin 235 Val1 Met met Gly Met Leu Giu Glu Ala 140 Lys Val1 Gin Tyr Leu 220 Lys Arg Gin Val Val1 Thr Asp Gly Val1 125 Thr Arg Ser Leu Leu 205 Ala Ala Gin Phe Asp Leu Lys Pro Ile 110 Val Asp Thr Ala Val1 190 Gly Ala Ser Asn Thr Thr Ile Ala Val Val Ala Ser Gly Asp 175 Ile Ala His Ala Thr Ala Pro Asn Ile Ala Asp Leu Val Gly 160 Arg Gly Thr Ala Pro 240 WO 01/00843 WO 0100843PCTJIBOO/00923 Gly Ser Phe Ala Val Ala Phe Ile Asp Ala 245 250 Leu Tyr Asp Val Asp Ala 255 Gln Ala Val Ala Ser Leu Val Asp Val 260 265 Arg Giu Ala <210> 535 <211> 1023 <212> DNA <213> Corynebacterium glutamicun <220> <221> <222> <223>

CDS

(101)..(1000) RXA0 0838 <400> 535 tcgtctaata gtgctgccaa tccaccggcc attgatgact cctttgtaga gaaggggtag tgcttacaaa tcttatctgt gctcaggcaa gatagcaggt atg aaa att gcg atc Met Lys Ile Ala Ile gtt ggc gct ggt Val Gly Ala Gly gtt ggt gga tat Val Gly Gly Tyr gga gcg ttg tta Gly Ala Leu Leu caa gaa Gin Glu tct ggt gca Ser Gly Ala ttg aag tct Leu Lys Ser atc acg atg gtt Ile Thr Met Val cgt gga cga aca Arg Gly Arg Thr tta gaa gcc Leu Giu Ala gaa cgc tac Glu Arg Tyr 211 259 aaa gga ctc cac Lys Gly Leu His atc Ile 45 aac gat gca aga Asn Asp Ala Arg ggc Gly gta cca Val Pro att cct gca gtt Ile Pro Ala Val agc gtg caa gaa Ser Val Gin Giu aaa gat gca gat Lys Asp Ala Asp gta Val gtg atg att gct Val Met Ile Ala aaa gca tta tcg Lys Ala Leu Ser cgg tct Arg Ser 80 tta gat Leu Asp ctc gct Leu Ala act cag Thr Gin 100 gaa ctt ttg ggt Giu Leu Leu Gly ata cct gcg aat Ile Pro Ala Asn gtg gtc gcg att Val Val Ala Ile aat tcg att Asn Ser Ile cgt gtg tgg Arg Val Trp, 120 gaa Glu 105 tct gct gat cta Ser Ala Asp Leu gcg aag agt atc Ala Lys Ser Ile ggt gct gat Gly Ala Asp 115 gag ggg cca Giu Gly Pro cct ggt gtg gtt Pro Gly Val Val ggg ttc ttt gtt Gly Phe Phe Val cat His 130 gcc tca Ala Ser 135 gtg tca tac aag Val Ser Tyr Lys ggc cca ctg tcc Gly Pro Leu Ser acg ttt ggt gat Thr Phe Gly Asp WO 01/00843 tct ggt gaa ctt tct agg caa, Ser Giy Giu Leu Ser Arg Gin 150 155 att gac gga gtt ctg cat ccc Ile Asp Gly Val Leu His Pro 170 gcc atg ttc gta gag gtt ttc Ala Met Phe Val Giu Val Phe 185 caa tta ggt acc ttg cgt acg Gin Leu Gly Thr Leu Arg Thr 200 atg gaa gag gtg gct gag gtg Met Giu Giu Val Aia Glu Val 215 220 agc gat gcg gtg gag cgc acc Ser Asp Ala Val Giu Arg Thr 230 235 aat tcg acg agt tcg atg cag Asn Ser Thr Ser Ser Met Gin 250 gag ctt gag gct cag aca ggt Giu Leu Glu Ala Gin Thr Giy 265 ggt gtg aaa act ccg ctt cat Giy Val Lys Thr Pro Leu His 280 aaa gaa gag gaa aat tca ctt 1023 Lys Giu Giu Giu Asn Ser Leu 295 300 <210> 536 <211> 300 <212> PRT <213> Corynebacterium glutan <400> 536 Met Lys Ile Ala Ile Val Gly 1 5 Ala Leu Leu Gin Giu Ser Gly Arg Thr Leu Glu Ala Leu Lys Arg Giy Glu Arg Tyr Val Pro 55 Leu Lys Asp Ala Asp Val Vai gca Ala at t I le ggg Gly 190 ttt Phe cgc Arg aat Asn gat Asp att Ile 270 ct t agc Ser t tg Leu 175 ttg Leu agg Arg gcg Ala ttt Phe ttg Leu 255 gtg Val1 att gaa cag Giu Gin gtg tgg Val Trp, ttc gtc Phe Val 195 ctg gaa Leu Glu 210 gtt gcg Val Ala cgg atg Arg Met gga gtg Gly Val gcg cac Ala His 275 ggt ctt Gly Leu 290 PCTIBOO/00923 gcc ggt 595 Ala Gly 165 gag aaa 643 Giu Lys 180 gaa aag 691 Giu Lys gcc ttg 739 Ala Leu ttg ccg 787 Leu Pro cct gag 835 Pro Giu 245 gct agt 883 Ala Ser 260 aaa gtg 931 Lys Val aag ctg 979 Lys Leu Asp Leu Ile Tyr Ala tagggataga atcaagatcc atg Gly Asp 25 Lys Pro Ilie 771 Ala Val Gly Gly Tyr Phe Gly 10 Ile Thr Met Val Ala Arg Gly Gly Leu His Ile Asn Asp Ala Ala Val Ala Ser Val Gln Glu Ala Thr Lys Ala Leu Ser Arg WO 01/00843 Ser Leu As Val Ala Il Ser Ile G1 11 Val His G1 130 Tyr Thr Ph 145 Leu Glu Gi Asp Val Tr Ala Phe Va 19 Ser Leu Gi 210 Gly Val Al 225 Asp Arg Me Ala Gly Va Ala Ala Hi 27 Leu Thr 100 Ala Gly Gly Ala Glu 180 Glu Ala Leu Pro Ala 260 Lys Ala Gin Asp Pro Asp Gly 165 Lys Lys Leu Pro Glu 245 S er Val Pro Ala Asn Asp Val1 Gly 140 Gin Pro Phe Thr Val 220 Thr Gln Gly His Leu Arg 125 Gly Phe Asp Gly His 205 Ala Met Arg Ala Asp 285 PCTIIBOO/00923 Ser Val Ala Lys Phe Phe Leu Ser Ser Thr 160 Leu Val 175 Leu Gly Arg Ala Ala Ala Phe Ala 240 Leu Ala 255 Val Arg Ile Tyr Ala Gly Leu Lys Leu Lys Glu Glu Glu Asn Ser Leu 290 295 300 <210> 537 <211> 693 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (101)..(670) <223> RXA02400 <400> 537 aggtgccccc aacgttgcct gttgactgca aattttccga aagaatccat aaactacttc tttaagtcgc cagattaaag tcgtcaatga aaggacatac atg tct att tcc cgc 115 Met Ser Ile Ser Arg 1 WO 01/00843 WO 0100843PCTIBOOIOO923 acc gtc ttc ggc Thr Val Phe Gly gca gcc acc gca Ala Ala Thr Ala gcc ctg Ala Leu tct gca gct Ser Ala Ala ctc gtt Leu* Val gcg tgt tct Ala Cys Ser cct cac cag cag Pro His Gin Gin tcc cca gtc cag Ser Pro Val Gin cgc acc aat Arg Thr Asn gag atc ttg act act tct cag Giu Ile Leu Thr Thr Ser Gin acc tct tcc gca acg act act Thr Ser Ser Ala Thr Thr Thr aac Asn 45 cca act tct gcg Pro Thr-Ser Ala agc agc acc tca Ser Ser Thr Ser gaa gag gac gta Giu Giu Asp Val tcc tca gct cct Ser Ser Ala Pro gag Glu atc gtt gtt tca Ile Val Val Ser gca gcg ttg gtg Ala Ala Leu Val ggt gag cag gtt Gly Giu Gln Val ttc gaa atc tct Phe Glu Ile Ser ctt gat cca gag Leu Asp Pro Glu ggc tac tac gca Gly Tyr Tyr Ala gcg atc Ala Ile 100 tgc gat tcc Cys Asp Ser gaa atg gct Glu Met Ala 120 gcg aac cct ggt Ala Asn Pro Gly aac Asn 110 cca gtt cct tct Pro Val Pro Ser tgc acc ggc Cys Thr Gly 115 tcc cag ccc Ser Gin Pro gat ttc acg tcc Asp Phe Thr Ser cag Gin 125 gca tgg ttg agc Ala Trp Leu Ser ggc gcg act gta gag atc Gly Ala Thr Val Glu Ile 135 gaa gac ggc acc gca act gtg gag ctt Giu Asp Gly Thr Ala Thr Val Glu Leu 145 gaa Giu 150 gct acc gca acc Ala Thr Ala Thr act ggc ttg gac Thr Gly Leu Asp acc act cag gct Thr Thr Gin Ala gta gcg aag gtc Val Ala Lys Val ggc gat cat acc Gly Asp His Thr gaa Glu 175 ggt ttc cgc gat Gly Phe Arg Asp gtt gct Val Ala 180 595 643 690 gaa gtc cca Giu Val Pro act ttc gca gcc Thr Phe Ala Ala gct taagttttct taaaacgcac Ala 190 tca <210> 538 <211> 190 <212> PRT <213> Corynebacteriui giutamicum <400> 538 Met Ser Ile Ser Arg Thr Val Phe Gly 1 5 Ile Ala Ala Thr Ala Ala Leu 10 WO 01/00843 Ser Ala Ala Val Gin Arg Ala Ser Ser Val Giu Giu Gly Glu Gin Tyr Tyr Ala Pro Ser Cys 115 Ser Asn Ser 130 Ala Thr Val 145 Thr Thr Gin Phe Arg Asp Leu Thr Thr Asp Val Ala 100 Thr Gin Giu Ala Val 180 Ala Giu Thr Glu 70 Phe Cys Glu Gly Glu 150 Val1 Glu Cys Ile Ser 55 Ile Glu Asp met Ala 135 Aia Ala Val1 Ser Leu 40 Ser Vai Ile Ser Ala 120 Thr Thr Lys Pro Pro Thr Ala Val Ser Val 105 Asp Val Ala Val Val 185 Gin Gin Thr Ala Asp Pro Ser Ala 140 Thr

ASP

Ala Gin Asn Ser Ala Pro Gly Gin 125 Giu Gly Hius Ala PCTIiBOO/00923 Ser Pro Thr Ser Ala Pro Val ASP Gly Gly Pro Vai Trp Leu Gly Thr ASP Cys 160 Glu Gly 175 <210> 539 <211> 1528 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1528) <223> RXN01209 <400> 539 cagattgcag cacagaaggc atcggcgcca ggcagctttg ctttatgacg tggatgccca ggctgtggcc tcgttggttg gaa aag tac gtg act gat ttt tct ttg Glu Lys Tyr Val Thr Asp Phe Ser Leu ttg ggt ggc ggg cca aaa aaa gta gct Leu Gly Gly Gly Pro Lys Lys Val Ala 30 tcc ggc gga gtt tct gtg gtg cag ctg Ser Gly Gly Val Ser Val Val Gin Leu tat ctg Tyr Leu 15 gga att Giy Ilie cgc gat Arg Asp cggtggcgtt tattgatgcg atg tgc gag agg cct Met Cys Giu Arg Pro 1 gtc acc gat ccc gtt Val Thr Asp Pro Val gtt gac agc gca att Val Asp Ser Ala Ile aag aac tca ggc gtg Lys Asn Ser Gly Val WO 01/00843 WO 0100843PCTJIBOO/00923 ctc tgc gat gct Leu Cys Asp Ala gaa gat Giu Asp gtt cgt gcg gca Val Arg Ala Ala aag gag ctg aaa Lys Giu Leu Lys cgc Arg ggg gtg gcg ctt Gly Val Ala Leu gt~c aac gat tac Val Asn Asp Tyr gat atc gcc gtt Asp Ile Ala Val 307 355 403 451 ctg ggt ctt cac Leu Gly Leu His cac att ggt caa His Ile Gly Gin gat aca cct tat Asp Thr Pro Tyr acg caa Thr Gin 100 gca cgg gag Ala Arg Giu aac ctg gat Asn Leu Asp 120 ctt cca gct cat ctt gaa ttg ggt Leu Pro Ala His Leu Giu Leu Gly 110 ttg agc att gaa Leu Ser Ile Giu 11 caa ttg cat gct Gin Leu His Ala atc gcg cag tgc Ile Ala Gin Cys gcc Ala 130 gag act ggt Giu Thr Gly gtg gca Val Ala 135 ttg ccc gat gtg att ggc att ggt ccg Leu Pro Asp Val Ile Gly Ile Gly Pro gtg Val 145 gcc tct act gcg Ala Ser Thr Ala aaa cca gat gcg gca ccc gca ttg ggt Lys Pro Asp Ala Ala Pro Ala Leu Gly 155 gtg Val 160 gag ggc atc gct Glu Gly Ile Ala atc gcc gct gta Ile Ala Ala Val caa gac cac ggc Gin Asp His Giy gca tca gta gct Ala Ser Val Ala att gga Ile Gly 180 ggc gtt ggt Gly Val Gly ggt ctg tgc Gly Leu Cys 200 cgc aac gcg gcc Arg Asn Ala Ala ctc gct gct acg Leu Ala Ala Thr ccc atc gac Pro Ile Asp 195 cca gca gct Pro Ala Ala 643.

6 91 739 gtg gtc tct gaa Val Val Ser Giu atg acc gcc gcc Met Thr Ala Ala gcg gca Ala Ala 215 act cgc ctg cgg Thr Arg Leu Arg gct ttt caa cct Ala Phe Gin Pro ttc tcg cct gaa Phe Ser Pro Glu act Thr 230 caa act gaa ctc Gin Thr Giu Leu caa aca gaa ctc Gin Thr Giu Leu gga gcc ttc gtg Gly Ala Phe Val aa t Asn 245 tcg cct tct gcc Ser Pro Ser Ala cgt gtg ttg tct att gca ggc act gat Arg Val Leu Ser Ile Ala Gly Thr Asp 255 ccc aca Pro Thr 260 ggt ggt gca Gly Gly Ala ggc tac ggc Gly Tyr Gly 280 gg t Gly 265 att cag gct gat Ile Gin Ala Asp aag tcc att. gca Lys Ser Ile Ala gca ggt ggc Ala Gly Gly 275 aac acc cac Asn Thr His atg tgc gtt gtg Met Cys Val Val acc Thr 285 tcg ctg gtc gcg Ser Leu Val Ala WO 01/00843 WO 0100843PCTJLBOOI00923 ggc gtC 1027 Gly Val 295 ctg gaa 1075 Leu Glu 310 atg ttg 1123 Met Leu tcc cac 1171 Ser His agc ggt 1219 Ser Gly ctg gcc 1267 Leu Ala .375 gtg ttg 1315 Val Leu 390 cag gct 1363 Gin Ala ggt gga 1411 Gly Gly gac ggc 1459 Asp Gly tcc cat 1507 Ser His 455 gcc gcc 1528 Ala Ala 470 aac acg atc cac Asn Thr Ile His gcg gtc ttt tcc Ala Val Phe Ser 315 acc cca ccc ttg acc ttt ttg gaa gaa cag Thr Pro Pro Leu Thr Phe Leu Glu Glu Gin 300 305 gat gtc acc gtc gat gcc atc aag ctc ggc Asp Vai Thr Val Asp Ala Ile Lys Leu Gly 320 325 ggc tct gcc Giy Ser Ala gag cac Giu His 345 gat cgc Asp Arg 330 gg t Gly c ta gac acc gtc gat ctg Asp Thr Val Asp Leu 335 ccc gtg gtg ctt gat Pro Val Val Leu Asp 350 ctg gat gcg agc gct Leu Asp Ala Ser Ala ccc gct tca tgg ctt ggt Ala Ser Trp Leu Gly 340 gtc atg atc gcc acc Val Met Ile Ala Thr 355 gaa gaa tcg ctg Giu Glu Ser Leu cgc cgc Arg Arg 365 370 gtg cac gtc gat gtg Val His Val Asp Val 380 tgc gac agt gct cct Cys Asp Ser Ala Pro gtc acc ccg aat atc ccc gaa ctt gcc Val Thr Pro Asn Ile Pro Glu Leu Ala 385 gcc atc acc atg gat gag gcc att gct Ala Ile Thr Met Asp Giu Ala Ile Ala cag gga ttt Gin Giy Phe 410 cat ctg act His Leu Thr 395 gcg Ala cgg act cat gac Arg Thr His Asp 415 atc gtc att gtc aag Ile Val Ile Val Lys 420 425 tcg gtg Ser Val ggc gcg ctt Gly Ala Leu cag gtg gaa Gin Val Glu 445 t tc Phe gct gat aac gct gtc gtg cgc ccc Ala Asp Asn Ala Val Vai Arg Pro 430 435 aac ctg cgt gtc aac acc acc aac Asn Leu Arg Val Asn Thr Thr Asn 450 tct gcg tca ctt gcc acc aag atc Ser Ala Ser Leu Ala Thr Lys Ile aca ggc tgt tcg ctc Thr Giy Cys Ser Leu 460 ggc gaa agc gtg gaa Gly Glu Ser Val Glu 475 WO 01/00843 WO 0100843PCT/iBOO/00923 <210> 540 <211> 476 <212> PRT <213> Corynebacterium giutanicun <400> 540 Met Cys Glu Arg Pro Giu 1 Val Val1 Lys Giu Asp Thr Giy Cys Val1 145 Glu Ser Ala Ala Thr 225 Gly Gly Ile Thr Asp Asn Leu Ile Pro Leu Al a 130 Ala Gly Val1 Thr Asn 210 Phe Ala Thr Ala Asp Ser Ser Cys Ala Tyr Ser 115 Glu Ser Ile Ala Pro 195 Pro Ser Phe Asp Ala 275 5 Val1 Ile Val1 Ala Giu Gin Giu Gly Ala Glu 165 Gly Asp Ala Giu Asn 245 Thr Gly Lys Gly Gly Asp 55 Gly Gly Arg Leu Ala 135 Lys Ala Val Leu Ala 215 Gin Pro Gly Tyr Tyr Val Thr 10 Gly Gly Pro Giy Val Ser 40 Val Arg Ala Val Ala Leu Leu His Leu 90 Giu Leu Leu 105 Asp Gin Leu 120 Leu Pro Asp Pro Asp Ala Ala Val Ala 170 Gly Leu Arg 185 Cys Val Vai 200 Thr Arg Leu Thr Giu Leu Ser Ala Pro 250 Ala Gly Ile 265 Gly Met Cys 280 Asp Lys Val Al a Val 75 His Pro His Val Ala 155 Gin Asn Ser Arg Ser 235 Arg Gin Val Phe Lys Val Ala Val Ile Ala Ala Ile 140 Pro Asp Ala Giu Thr 220 Gin Val Ala Val1 Ser Val Gin Lys Asn Gly His Val 125 Gly Ala His Ala Ile 205 Ala Thr Leu Asp Thr 285 Leu Al a Leu Giu Asp Gin Leu 110 Ile Ile Leu Gly Giu 190 Met Phe Glu Ser Leu 270 Ser Tyr Gly Arg Leu Tyr Gly Giu Ala Gly Gly Ile 175 Leu Thr Gin Leu Ile 255 Lys Leu Leu Ile Asp Lys Leu Asp Leu Gln Pro Val1 160 Ala Ala Ala Pro Gin 240 Ala Ser Val1 WO 01/00843 PCT[IB0OOO923 Ala Gin Asn Thr His Gly Val Asn Thr Ile His Thr Pro Pro Leu Thr 290 295 300 Phe Leu Glu Glu Gin Leu Giu Ala Val Phe Ser Asp Val Thr Val Asp 305 310 315 320 Ala Ile Lys Leu Gly Met Leu Gly Ser Ala Asp Thr Val Asp Leu Val 325 330 335 Ala Ser Trp Leu Giy Ser His Giu His Giy Pro Val Val Leu Asp Pro 340 345 350 Val Met Ile Ala Thr Ser Giy Asp Arg Leu Leu Asp Ala Ser Ala Glu 355 360 365 Giu Ser Leu Arg Arg Leu Ala Val His Val Asp Val Val Thr Pro Asn 370 375 380 Ile Pro Glu LeU Ala Val Leu Cys Asp Ser Ala Pro Ala Ile Thr Met 385 390 395 400 Asp Glu Ala Ile Ala Gin Ala Gin Gly Phe Ala Arg Thr His Asp Thr 405 410 415 Ilie Val Ile Val Lys Gly Gly His Leu Thr Gly Ala Leu Ala Asp Asn 420 425 430 Ala Val Val Arg Pro ASP Gly Ser Val Phe Gin Val Giu Asn Leu Arg 435 440 445 Val Asn Thr Thr Asn Ser His Gly Thr Gly Cys Ser Leu'Ser Ala Ser 450 455 460 Leu Ala Thr Lys Ile Ala Ala Gly Giu Ser Val Giu 465 470 475 <210> 541 <211> 1528 <212> DNA <213> Corynebacterium giutamicum <2 <221> CDS <222> (101)..(1528) <223> FRXA01209 <400> 541 cagattgcag cacagaaggc atcggcgcca ggcagctttg cggtggcgtt tattgatgcg ctttatgacg tggatgccca ggctgtggcc tcgttggttg atg tgc gag agg cct 115 Met Cys Giu Arg Pro 1 gaa aag tac gtg act gat ttt tct ttg tat ctg gtc acc gat ccc gtt 163 Giu Lys Tyr Val Thr Asp Phe Ser Leu Tyr Leu Val Thr Asp Pro Val 15 ttg ggt ggc ggg cca aaa aaa gta gct gga att gtt gac agc gca att 211 Leu Gly Gly Gly Pro Lys Lys Val Ala Gly Ile Val Asp Ser Ala Ile 30 WO 01/00843 WO 0100843PCTIBOOIOO923 *tcc ggc gga gtt tct gtg gtg cag ctg cgc gat aag Ser Gly Gly Val Ser Vai Val Gin Leu Arg Asp Lys 45 gaa Giu cgc Arg ctg Leu gca Ala aac Asn gtg Val acc Thr 150 atc Ilie ggc Gly ggt Gly gcg Ala act Thr 230 t cg Ser ggt Gly gat Asp ggg Gly gg t Gly cgg Arg ctg Leu gca Ala 135 aaa Lys gcc Aia gt t Val ctg Leu gca Ala 215 caa Gin cct Pro ggt Gly cgt Arg gcg Ala cac His ctg Leu 105 caa Gin ccc Pro gat Asp gta Val cta Leu 185 gtg Val cgc Arg gaa Giu gcc Ala gg t Gly 265 gcg Ala ctt Leu ctg Leu ct t Leu t tg Leu gat Asp gcg Ala gct Ala 170 cgc Arg gtc Val ctg Leu c tc Leu cca Pro 250 att Ile gca Ala gtt Val cac His c ca Pro cat His gtg Val1 gca Ala 155 caa Gin aac Asn tc t Ser cgg Arg tct Ser 235 cgt Arg cag Gin gaa Giu ga t Asp aca Thr ggt Gly tgc Cys gtg Val 145 gag Giu t ca Ser gc t Ala gcc Ala act Thr 225 gga Gly ggc Gly att Ile aac tca ggc Asn Ser Gly ctc tgc gat Leu Cys Asp atc gcc gtt Ile Ala Val cct tat acg Pro Tyr Thr 100 ttg agc att Leu Ser Ile 115 gcc gag act Ala Giu Thr 130 gcc tct act Ala Ser Thr ggc atc gct Gly Ile Ala gta gct att Val Ala Ile 180 acg ccc atc Thr Pro Ile 195 aat cca gca Asn Pro Ala 210 ttc tcg cct Phe Ser Pro gcc ttc gtg Ala Phe Val act gat ccc Thr Asp Pro 260 gca gca ggt Ala Ala Gly 275 WO 01/00843 WO 0100843PCTA03OO/00923 ggc tac Gly Tyr ggc gtc 1027 Gly Val 295 ctg gaa 1075 Leu Glu 310 atg ttg 1123 Met Leu tcc cac 1171 Ser His agc ggt 1219 Ser Gly ctg gcc 1267 Leu Ala 375 gtg ttg 1315 Val Leu 390 cag gct 1363 Gin Ala ggt gga 1411 Gly Gly gac ggc 1459 Asp Gly tcc cat 1507 Ser His 455 gcc gcc 1528 ggc atg tgc gtt Gly Met Cys Val acc tcg ctg gtc gcg caa aac acc cac 979 Thr Ser Leu Val Ala Gin Asn Thr His' aac acg atc cac acc Asn Thr Ile His Thr 300 gcg gtc ttt tcc gat Ala Val Phe Ser Asp cca ccc ttg acc ttt Pro Pro Leu Thr Phe 305 gtc acc gtc gat gcc Val Thr Vai Asp Ala 290 ttg gaa gaa cag Leu Giu Giu Gin atc aag ctc ggc Ile Lys Leu Gly 325 tca tgg ctt ggt Ser Trp Leu Gly 340 atg atc gcc acc ggc tct gcc Gly Ser Ala 330 gag cac ggt Giu His Gly acc gtc gat ctg Thr Val Asp Leu 335 gtg gtg ctt gat Val Val Leu Asp ccc Pro ccc gtc 345 gat cgc Asp Arg 350 355 cta ctg gat gcg Leu Leu Asp Ala 365 gtc gat gtg gtc Val Asp Val Val agc gct gaa gaa tcg ctg cgc cgc Ser Ala Giu Glu Ser Leu Arg Arg 370 acc ccg aat atc ccc gaa ctt gcc Thr Pro Asn Ile Pro Glu Leu Ala cac His 380 tgc gac agt gct Cys Asp Ser Ala 395 cag gga ttt gcg Gin Gly Phe Ala 410 cct gcc atc acc atg Pro Ala Ile Thr Met 400 cgg act cat gac acc Arg Thr His Asp Thr 415 385 gat gag Asp Glu gcc att gct Ala Ile Ala 405 atc gtc att gtc Ile Val Ile Val 420 aag Lys cat ctg His Leu 425 tcg gtg Ser Val 440 ggc aca Gly Thr act ggc gcg ctt gct Thr Gly Ala Leu Aia 430 ttc cag gtg gaa aac Phe Gin Val Glu Asn 445 ggc tgt tcg ctc tct Gly Cys Ser Leu Ser gat aac Asp Asn ctg cgt Leu Arg gct gtc gtg cgc ccc Ala Val Vai Arg Pro 435 gtc aac acc acc aac Val Asn Thr Thr Asn 450 gcg tca ctt gcc acc aag atc Ala Ser Leu Ala Thr Lys Ile 465 460 ggc gaa agc gtg gaa WO 01/00843 Ala Ala Gly Glu Ser Val Glu 470 475 <210> 542 <211> 476 <212> PRT <213> Corynebacteriun glutamicum <400> 542 Met Cys Glu Arg Pro Giu Lys Tyr Val PCT/IBOO/00923 1 Vai Val1 Lys Glu Asp Thr Gly Cys Val1 145 Glu Ser Ala Ala Thr 225 Gly Gly Leu Ser Giu Arg 70 Leu Ala Asn Val Thr 150 Ile Gly Gly Ala Thr 230 Ser Gly Gly Gly Asp Giy Gly Arg Leu Ala 135 Lys Ala Val Leu Ala 215 Gin Pro Gly Gly Val Arg Ala His Leu 105 Gin Pro Asp Val Leu 185 Val Arg Glu Ala Gly 265 Thr 10 Pro Ser Ala Leu Leu Leu Leu Asp Ala Ala 170 Arg Val1 Leu Leu Pro 250 Ile Ser Val Gin Lys Asn Gly His Val 125 Gly Ala His Ala Ile 205 Ala Thr Leu Asp Leu Ala Leu Giu Asp Gin Leu 110 Ile Ile Leu Gly Giu 190 Met Phe Glu Ser Leu 270 Tyr Gly Arg Leu Tyr Gly Glu Ala Gly Gly Ile 175 Leu Thr Gin Leu Ile 255 Lys Leu Ile Asp Lys Leu Asp Leu Gin Pro Val1 160 Ala Ala Ala Pro Gin 240 Ala Ser WO 01/00843 PCT/IB00100923 Ilie Ala Ala Giy Gly Gly Tyr Giy Met Cys Val Vai Thr Ser Leu Val 275 280 285 Ala Gin Asn Thr His Gly Val Asn Thr Ile His Thr Pro Pro Leu Thr 290 295 300 Phe Leu Giu Giu Gin Leu Giu Ala Val Phe Ser Asp Val Thr Val Asp 305 310 315 320 Ala Ile Lys Leu Gly Met Leu Gly Ser Ala Asp Thr Val Asp Leu Val 325 330 335 Ala Ser Trp Leu Gly Ser His Giu His Gly Pro Val Vai Leu Asp Pro 340 345 350 Val Met Ile Ala Thr Ser Gly Asp Arg Leu Leu Asp Ala Ser Ala Giu 355 360 365 Giu Ser Leu Arg Arg Leu Ala Val His Val Asp Val Val Thr Pro Asn 370 375 380 Ile Pro Giu Leu Ala Val Leu Cys Asp Ser Ala Pro Ala Ile Thr Met 385 390 395 400 Asp Giu Ala Ilie Ala Gin Ala Gin Gly Phe Ala Arg Thr His Asp Thr 405 410 415 Ile Val Ilie Val Lys Gly Gly His Leu Thr Gly Ala Leu Ala Asp Asn 420 425 430 Ala Val Val Arg Pro Asp Gly Ser Val Phe Gin Val Giu Asn Leu Arg 435 440 445 Val Asn Thr Thr Asn Ser His Gly Thr Gly Cys Ser Leu Ser Ala Ser 450 455 460 Leu Ala Thr Lys Ile Ala Ala Gly Glu Ser Val Glu 465 470 475 <210> 543 <211> 723 <212> DNA <213> CorynebacteriumT glutanicuxn <220> <221> CDS <222> (101)..(700) <223> RXN01413 <400> 543 tttgccctat tggaagaagt aaattccaca ccttcacttt ccaatacttc ttttggtgag cgggtttcct cagcgtttaa ccatctgaaa ccatctgaga ttg acc cat ctg ttc 115 Leu Thr His Leu Phe 1 tta gaa ctc gat gag cgt tta gta ctg ggt gtt cag caa gat ggt tac 163 Leu Glu Leu Asp Giu Arg Leu Val Leu Gly Val Gin Gin Asp Gly Tyr 15 WO 01/00843 caa tgg act Gin Trp, Thr tcg ccc aat Ser Pro Asn PCT/IB00100923 cat ttg ttc cgg His Leu Phe Arg cca ctg caa cat Pro Leu Gin His ctc cgt aac Leu Arg Asn gaa ctt tat Giu Leu Tyr 211 259 gac ctg cag gga ASP Leu Gin Gly ttg Leu aag ata cga tgg Lys Ile Arg Trp tcc aca Ser Thr acg ggg aaa gat Thr Giy Lys Asp caa Gin ggg gta gaa ctt Giy Vai Giu Leu cct caa gca acc Pro Gin Ala Thr gtt acc cca aat Val Thr Pro Asn ttc gag gct tcc acc ctc tcc ggc ctt Phe Giu Aia Ser Thr Leu Ser Giy Leu 355 aag ctt gag acc Lys Leu Giu Thr gag gac ctc aag Giu Asp Leu Lys gct gcc cgc ctc Ala Aia Arg Leu att tat Ile Tyr 100 gag caa ggc Giu Gin Giy ggc gag aac Gly Giu Asn 120 ccc Pro 105 cag tac gta gtc Gin Tyr Vai Val gtc Val 110 aag ggt ggc atg Lys Gly Gly Met gac ttc ccc Asp Phe Pro 115 gcc gtg gat gtg Ala Vai Asp Vai ctt Leu 125 ttc gac gga tcc tcc tac cac gtc Phe Asp Gly Ser Ser Tyr His Vai 130 499 ttc tct Phe Ser 135 gag cca aag att Giu Pro Lys Ile gg t Gly 140 gaa gag cgc gtt Giu Glu Arg Val tcc S er 145 ggc gca gtc tgc Gly Ala Vai Cys acc Thr 150 ttc gca gct gtt Phe Ala Ala Val acc gca gag cta gca aag ggt gct gag Thr Ala Glu Leu Ala Lys Gly Ala Giu 160 gta gac cca gtg Vai Asp Pro Val gca Ala 170 acc gca aag cgt Thr Ala Lys Arg gtc acc cgt gcg Val Thr Arg Aia gtt caa Vai Gln 180 gat gct gtt Asp Ala Val gac aac aag ASP Asn Lys 200 gca Ala 185 tcc aac gca cct Ser Asn Ala Pro ttt Phe 190 acc tcc gta tgg Thr Ser Val Trp ctt gct gag Leu Ala Glu 195 tagagtttta aaataccgat caa <210> 544 <211> 200 <212> PRT <213> Corynebacteriun giutamicum <400> 544 Leu Thr His Leu Phe Leu Glu Leu Asp 1 Gin Gin Asp Gly Tyr Gin Trp Thr Glu 25 Glu Arg Leu Vai Leu Gly Vai His Leu Phe Arg Leu Pro Leu WO 01/00843 Gin His Leu Trp Cys Giu Leu Pro Gin Leu Ser Giy Ala Arg Leu Gly Met Asp 115 Ser Ser Tyr .130 Ser Gly Ala 145 Lys Gly Ala Thr Arg Ala Asn Tyr Thr Giu Tyr Pro Val Cys Val 165 Gin Gly Gin Phe Asp Vai Val1 Gly 140 Thr Ala Ala Leu Gly Giu Leu Val1 Leu 125 Giu Ala Lys Pro Lys Val1 Ala Lys Val1 110 Phe Glu Giu Arg Phe 190 PCT/I1BOO/00923 Ile Arg Glu Leu Ser Thr Giu Ala Lys Gly Asp Gly Arg Val Leu Ala 160 Val Vai 175 Thr Ser Vai Trp Leu Ala Giu Asp Asn Lys 195 200 545 <211> 795 <212> DNA <213> Corynebacterium glutainicum <22 0> <221> CDS <222> (101)..(772) <223> RXN01617 <400> 545 tcagaagcta ccggcggcgc cggcatccag gttgatttga tgtttatggc attgggtcca tcacatgctt gggtggcctt act gga atc acc gtt ttg tcc cgg ttt gat gcg Thr Gly Ilie Thr Val Leu Ser Arg Phe Asp Ala 15 cag att gag gcc gcc acc gca gcg cac gat ctt Gin Ilie Giu Ala Ala Thr Ala Ala His Asp Leu 30 ggt atg ttg ggt act cct gca acg atc gat act Gly Met Leu Gly Thr Pro Ala Thr Ile Asp Thr 784 agaccttcca gcatttaaga ttg atc cta aag aca Leu Ile Leu Lys Thr 1 cag gtt atc gct aat Gin Val Ile Ala Asn gat gtg gtg aag atc Asp Val Val Lys Ilie gtg gca acc gct ttg Val Ala Thr Ala Leu WO 01/00843 WO 0100843PCTIiBOO/00923 gta ctg atc tgc Val Leu Ile Cys gag gaa Glu Glu aac agc ttc aag Asn Ser Phe Lys gtt gtc cta gac Val Vai Leu Asp aag Lys ggc cag gag ccc Gly Gin Giu Pro gcg gca ctt gat Ala Ala Leu Asp act Thr gac act gcc ctt Asp Thr Ala Leu gcg aag gtg ctg Ala Lys Val Leu cag gca acc gtg Gin Ala Thr Val gtt act Val Thr cca aac aac Pro Asn Asn ttc gag Phe Glu 100 gcc acc acc Ala Thr Thr aag gaa gca Lys Giu Ala 120 ctg Leu 105 tct ggc cta gac Ser Gly Leu Asp ctg gag acc atc Leu Glu Thr Ile gac gac ctg Asp Asp Leu 115 gcc cgc ctc att Ala Arg Leu Ile cat gag caa gga cct cag tac gtc gtt His Glu Gin Gly Pro Gin Tyr Val Val 125 130 cca ggc gac aac gct gtg gac gta ctt Pro Gly Asp Asn Ala Val Asp Val Leu 145 gtt aag Val Lys 135 ggt ggc atc gac Gly Gly Ilie Asp gac ggc acc gac Asp Gly Thr Asp cac gtg ttc tct His Val Phe Ser gaa G lu 160 cca aag atc ggc Pro Lys Ile Gly gag cgc gtc tcc Giu Arg Val Ser gct ggc tgt acc Ala Gly Cys Thr gca gct gtc atc Ala Ala Val Ilie acc gca Thr Ala* 180 gag ctg gcc Glu Leu Ala cgc gta gtc Arg Val Val 200 ggc aac tct gcc Gly Asn Ser Ala gat gca gtg acc Asp Ala Val Thr acc gct aag Thr Ala Lys 195 aac gca ccg Asn Ala Pro acc cgc gca gtg Thr Arg Ala Vai gac gct gtc gca Asp Ala Val Ala tcc Ser 210 ttt acc Phe Thr 215 cct tct gtg tgg ttg Ser Val Trp Leu gaa gac aac aag Giu Asp Asn Lys tagaaatctt aaacaagctc 792 <210> 546 <211> 224 <212> PRT <213> Corynebacteriun giutamicun <400>. 546 Leu Ile Leu Lys Thr Thr Gly Ile Thr Val Leu Ser Arg 1 5 10 Gin Val Ile Ala Asn Gin Ile Glu Ala Ala Thr Ala Ala 25 Phe Asp Ala His Asp Leu WO 01/00843 PCTIiBOO/00923 Lys Ile Gly Met Leu Gly Thr Pro Ala Thr Ile Asp Thr 40 Asp Val Pro Asp Pro Thr Pro Ala 145 Pro Ala Val1 Val Ala Val1 Thr Asn Ilie Gin 130 Val Lys Val Thr Val Thr Leu Ala Asn Asp 115 Tyr Asp Ilie Ile Thr 195 Ala Leu Ile Cys Leu Arg Phe Giu 100 Asp Leu Val Val Val Leu Giy Asp 165 Thr Ala 180 Ala Lys Giu Lys 70 Ala Ala Lys Vai Phe 150 Giu Giu Arg Giu 55 Gly Lys Thr Glu Lys 135 Asp Arg Leu Val1 Asn Gin Val1 Thr Ala 120 Gly Gly Val Ala Val 200 Ser Glu Leu Leu 105 Ala Gly Thr Ser Lys 185 Thr Phe Pro Pro 90 Ser Arg Ile Asp Gly 170 Giy Arg Lys Gly Gin Gly Leu Asp Tyr 155 Ala Asn Ala His Ala Ala Leu Ile Phe 140 His Gly Ser Val Val1 Ala Thr Asp His 125 Pro Val Cys Ala Lys 205 Val Leu Val1 Lys 110 Giu Gly Phe Thr Val 190 Asp Leu Asp Val Leu Gin Asp Ser Phe 175 Asp Ala Ala Ser Asn Ala Pro Phe Thr Ser Val Trp Leu Ala Giu Asp Asn Lys <210> 547 <211> 638 <212> DNA <213> Corynebacterium glutanicum <22 0> <221> CDS <222> <223> FRXA01617 <400> 547 gct aat cag att gag gcc gcc acc gca gcg cac gat ctt gat gtg gtg Ala Asn Gin Ile Glu Ala Ala Thr Ala Ala His Asp Leu Asp Val Val 1 5 10 aag atc ggt atg ttg ggt act cct gca acg atc gat act gtg gca acc Lys Ile Gly Met Leu Gly Thr Pro Ala Thr Ile Asp Thr Val Ala Thr 25 gct ttg gag gaa aac agc ttc aag cac gtt gtc cta gac ccg gta ctg Ala Leu Glu Glu Asn Ser Phe Lys His Val Val Leu Asp Pro Val Leu 48 96 144 WO 01/00843 WO 0100843PCTIiBOO/00923 40 atc tgc aag ggc cag gag ccc ggc Ile Cys Lys Gly Gin Giu Pro Gly 55 ctt cgc gcg aag gtg ctg cca cag Leu Arg Ala Lys Val Leu Pro Gin 70 ttc gag gcc acc acc ctg tct ggc Phe Giu Ala Thr Thr Leu Ser Gly gac ctg aag gaa gca gcc cgc ctc Asp Leu Lys Glu Ala Ala Arg Leu 100 gtc gtt gtt aag ggt ggc atc gac Val Val Val Lys Gly Gly Ile Asp 115 120 gta ctt ttc gac ggc acc gac tac Val Leu Phe Asp Gly Thr Asp Tyr 130 135 ggc gac gag cgc gtc tcc ggc gct Gly Asp Giu Arg Val Ser Gly Ala 145 150 acc gca gag ctg gcc aag ggc aac Thr Ala Giu Leu Ala Lys Gly Asn 165 gct aag cgc gta gtc acc cgc gca Ala Lys Arg Val Val Thr Arg Ala 180 gca ccg ttt acc tct gtg tgg ttg Ala Pro Phe Thr Ser Val Trp Leu 195 200 aaacaagctc cct <210> 548 <211> 205 <212> PRT <213> Corynebacterium glutamicun <400> 548 Ala Asn Gin Ilie Glu Ala Ala Thr 1 5 Lys Ile Gly met Leu Gly Thr Pro Ala Leu Giu Giu Asn Ser Phe Lys 40 Ile Cys Lys Gly Gin Giu Pro Gly gcg gca ctt gat act Ala Ala Leu Asp Thr gca acc gtg gtt act Ala Thr Val Val Thr 75 cta gac aag ctg gag Leu Asp Lys Leu Glu 90 att cat gag caa gga Ile His Giu Gin Gly 105 ttc cca ggc gac aac Phe Pro Gly Asp Asn 125 cac gtg ttc tct gaa His Val Phe Ser Glu 140 ggc tgt acc ttc gca Gly Cys Thr Phe Ala 155 tct gcc gtt gat gca Ser Ala Val Asp Ala 170 gtg aag gac gct gtc Val Lys Asp Ala Val 185 gcg gaa gac aac aag Ala Glu Asp Asn Lys act Thr aac Asn atc Ile cag Gin gtg Val aag Lys gtc Val acc Thr 175 tcc Ser gcc Ala aac Asn gac Asp tac Tyr gac Asp atc Ile atc Ile 160 acc Thr aac Asn 192 240 288 336 384 432 480 528 576 625 tagaaatctt Ala Ala His Asp Leu Asp Val Val 10 Ala Thr Ile Asp Thr Val Ala Thr 25 His Val Val Leu Asp Pro Val Leu Ala Ala Leu Asp Thr Asp Thr Ala 787 WO 01/00843 Leu Arg Ala Phe Glu Ala Asp Leu Lys Val Val Val 115 Val Leu Phe 130 Gly Asp Glu 145 Thr Ala Glu Ala Lys Arg Ala Pro Phe 195 PCTIBOO/00923

LYS

Thr Glu 100 Lys Asp Arg Leu Val1 180 Thr Val Thr Ala Gly Gly Val Ala 165 Val1 Ser Val Leu Gln Asp Ser 140 Phe Asp Al a Asn Thr Glu Gly Asn 125 Glu Ala Ala Val Lys 205 Asn Ile Gln Val Lys Val1 Thr 175 Ser Asn Asp Tyr Asp Ile Ile 160 Thr Asn <210> 549 <211> 915 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (101)..(892) <223> RXS01807 <400> 549 gctcaccgag ctggacacca agctccgcgc agtgcaggaa gcagtggctg gaactcggcg aggaaatcga gggctagttc gag Glu gcc Ala ctc Leu gc t Ala att tt Ilie Uf gtg 9t Val Vi gct gc Ala AJ ccc ac Pro T1 La cgc Arg ggc Gly gtg Val tta Leu gaacacggcg agctggaaat atg ccg tcg gca ggc Met Pro Ser Ala Gly 1 ttt gat cag cgc cgc Phe Asp Gln Arg Arg tat ggt tcc gtg ggg Tyr Gly Ser Val Gly ggc ctc cgc gtg gtc Gly Leu Arg Val Val cgt tat gca agt tct Arg Tyr Ala Ser Ser ~c gtg gtg ~r Val Val WO 01/00843 WO 0100843PCT/1BO0100923 gac gcg ctg caa Asp Ala Leu Gin cac His cgc cag cog atg Arg Gin Pro Met tcg Ser gac caa tgg oto Asp Gin Trp Leu ctg gtg gat ctg Leu Val Asp Leu att ato gat gag gtt too 8CC att tcc Ile Ile Asp Giu Val Ser Thr Ile Ser acc ggc Thr Gly 100 tat ttt acc Tyr Phe Thr aaa ato cgc Lys Ilie Arg 120 got tct cag gtg Ala Ser Gin Val gtg gtc got gcg Val Val Ala Ala tgg ctg cag Trp Leu Gin 115 gat coo atc Asp Pro Ile gaa acc cat ccg Giu Thr His Pro gtg cgc atc gtg Val Arg Ile Val atg ggg Met Gly 135 gac agt gac gtg Asp Ser Asp Val att tat gtc gcc Ile Tyr Val Ala gag atc gca acc Giu Ile Ala Thr gcc Ala 150 atc tgo cag gao Ilie Cys Gin Asp tta Leu 155 tgc cct otg got Cys Pro Leu Ala ac 0 Thr 160 gga. ato att ccc Gly Ile Ile Pro got tto gag oto too cac atg gtt ggc Ala Phe Giu Leu Ser His Met Val Gly 170 too ggo Ser Gly 175 gat cog ogo Asp Pro Arg tog otg Ser Leu 180 oto ggo cog Leu Gly Pro gtg ggc aco Val Gly Thr 200 ggc gag tgg ato ato ato aco ago gc Gly Giu Trp Ile Ile Ilie Thr Ser Ala 190 act gaa act Thr Giu Thr 195 gto cag gaa Val Gin Giu aco gto aco ogo Thr Val Thr Arg ato Ile 205 gtc aoo ogt gao Val Thr Arg Asp ato goc too goc aco gtc Ile Ala Ser Ala Thr Val 215 tao goc gca goa tta ato Tyr Ala Ala Ala Leu Ile 230 235 gat Asp 220 aoc aog goc aaa Thr Thr Ala Lys ggg Gly 225 goa ggc gao gtc Ala Gly Asp Val goc goc ctg oat Ala Ala L6U His aaa Lys 240 gat ttt tog ott Asp Phe Ser Leu ato Ile 245 gao goc goo Asp Ala Ala aaa gog ott Lys Ala Leu ago cac Ser His 250 goa too aao 800 Ala Ser Asn Thr tgo goo ggo otg Cys Ala Gly Leu cag 800 Gin Thr 260 taggtttogt ccgtctctga. oag <210> 550 <211> 264 <212> PRT <213> Corynebaoteriun giutamioun <400> 550 WO 01/00843 Met Pro Ser 1 Phe Asp Gin Tyr Gly Ser Gly Leu Arg Arg Tyr Ala Asp Ala Leu Thr Ilie Ser Ala Ala Trp 115 Val Val Asp 130 Asp Giu Ilie 145 Gly Ile Ile Asp Pro Arg Ser Ala Thr 195 Asp Ser Vai 210 Gly Ala Gly 225 Asp Phe Ser Ala Giy Leu Ala Arg Val1 Val Ser Gin Thr 100 Leu Pro Ala Pro Ser 180 Giu Gin Asp Leu Gin 260 Glu Asp Ser Val 55 Arg Val1 Phe Ile Gly 135 Ilie Phe Gly Giy Al a 215 Ala Ala Ala Gin Ile Pro Val Ser 75 Ile Ser His Val Leu 155 His Glu Thr Val Ile 235 Ala Arg Gly Val Leu Asp Ile Gin Pro Gly 140 Cys Met Trp Arg Asp 220 Ala Ser PCTIBOO/00923 Leu Giu Vai Val Asn Giu Met Pro Leu Ala Val Ser Val Vai Arg Ile Val Ala Ala Thr 160 Ser Gly 175 Ile Thr Thr Arg Ala Lys His Lys 240 Val Cys 255 <210> 551 <211> 622 <212> DNA <213> Corynebacterium giutarnicum <22 0> <221> CDS <222> (101)..(622) <223> RXCO1021 WO 01/00843 WO 0100843PCTIiBOO/00923 <400> 551 cgagaggctt ttttggctct aagcctttta gtcgtgcgaa cgaaatctta agcagcctcg gtgccaccga gatcgattgg tcgctgtaag gtatctgatt atg tcc agt tcc gaa Met Ser Ser Ser Glu agc tcg cgt tcc Ser Ser Arg Ser ggc tcg cag cca Gly Ser Gin Pro ccg tct gta cag Pro Ser Val Gin cct gaa Pro Giu cgc cgt gct Arg Arg Ala tcc caa caa Ser Gin Gin tca acg ggg gct Ser Thr Gly Ala gcg gca gct tcc Ala Ala Ala Ser aag gaa gct Lys Giu Ala agg acc gct Arg Thr Ala atg gac gct gcc Met Asp Ala Ala gtt ctt gag tgg Val Leu Giu Trp gtc gag Val Giu cag ctt tct gaa Gin Leu Ser Giu cgt gca gag atc Arg Ala Giu Ile gca ctg aat gtc Ala Leu Asn Val cct gtt cca gat Pro Val Pro Asp gca Ala gac act gga tca Asp Thr Gly Ser atg acc tac acc Met Thr Tyr Thr .aca gct gcg ttg Thr Ala Ala Leu gat Asp gaa gcg ctg aaa Giu Ala Leu Lys ggg gag ttg ggt Gly Glu Leu Gly gat gtc Asp Val 100 gca agg att Ala Arg Ile gga aat tct Gly Asn Ser 120 gag gct ttg gct Giu Ala Leu Ala ggt tct gtg cgt Gly Ser Val Arg gga gcc cga Gly Ala Arg 115 att gct cag Ile Ala Gin gga gta gtc ctt Gly Val Val Leu cag gtc ctt cgc Gin Val Leu Arg gct.

Ala 130 gca gct Ala Ala 135 gct gac ggg gtt Ala Asp Gly Val gat ggc cac aca Asp Gly His Thr caa gaa gcg cta Gin Giu Ala Leu att gct cgc tcc Ile Ala Arg Ser cta Leu 155 gtt gat cgc gca Val Asp Arg Ala aca gat cct gtg Thr Asp Pro Val ggc act gtt gtc Gly Thr Val Val act Thr 170 gtg ttg cgt tct 622 Val Leu Arg Ser <210> 552 <211> 174 <212> PRT <213> Corynebacterium giutanicum <400> 552 Met Ser Ser Ser Giu Ser Ser Arg Ser Giu Gly Ser Gin Pro Ala Pro WO 01/00843 WO 0100843PCTIIBOO/00923 Ser Ala Trp Asn Met Giu Val1 Arg Ile 145 Thr Giu Ala Ala Val Met Val1 Arg Gin Leu Giu 165 Arg Ser Vai Phe Thr Ala Gly Ala Ser 150 Gly Ala Gly Arg Thr Leu Ala Ser 125 Asp Asp Arg Pro Val Ala G ly Lys Val 110 Gin Gly Arg Ser <210> 553 <211> 1107 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1084) <223> RXN02246 <400> 553 tgccgacgct ggcgtggatg ccttcgttgc aggttccgct caacaaggcg atccaggagt tgcgagcact cgcgcagtaa gcg tta gat Ala Leu Asp agc cct aat Ser Pro Asn gtc gtt ggc Val Val Gly ctg gcc cac cac gtg Leu Ala His His Val ccg cca gtc ggc gct Pro Pro Val Gly Ala gtt ggc gcc acg gca Val Gly Ala Thr Ala 45 tca gat caa Ser Asp Gin 15 gtc att ttg Val Ile Leu 30 cct cct ggt Pro Pro Gly gtgtacggcg ctgaggatcc atg gat gtt gcg cac Met Asp Val Ala His 1 gtc cga ggc acc acc Val Arg Gly Thr Thr gac gcc gac ggc gag Asp Ala Asp Gly Giu ggc ccg cac gcc gaa Gly Pro His Ala Giu WO 01/00843 WO 0100843PCTIiBOO/00923 gtg gtg gcg ctt gca gct Val Val Ala Leu Ala Ala gga gtg cgt gcc Gly Val Arg Ala ggg ggc acg gcg Gly Gly Thr Ala g tg Val gtc acc ctc gag Val Thr Leu Glu tgc aac cat tac Cys Asn His Tyr ggc Gly cgc acg ggt cca Arg Thr Gly Pro tcc aag gcg ctt Ser Lys Ala Leu ctc Leu gac gcc ggg atc Asp Ala Gly Ile cac gtg ttt tac His Val Phe Tyr gcc aat Ala Asn 100 gcg gat ccc Ala Asp Pro gcg ggc gtc Ala Gly Val 120 ccg tca gcc gct Pro Ser Ala Ala ggc ggt gcc ttt Gly Gly Ala Phe ttg gcg gag Leu Ala Glu 115 gca ctg gag Ala Leu Glu gat acg cat ttt tta gat gag cgg atc Asp Thr His Phe Leu Asp Glu Arg Ile 125 agg Arg 130 ccc tgg Pro Trp 135 ctg gtt gcg acg cgt ctg ggc agg ccc Leu Val Ala Thr Arg Leu Gly Arg Pro 140 gtc acg ttg aag Val Thr Leu Lys gcg tcc acc gtg Ala Ser Thr Val ggt ttt gct ggt Gly Phe Ala Gly gcc acc gat ggc Ala Thr Asp Gly 160 ttt gtg cac gaa Phe Val His Glu acc Thr cag tgg att acc Gin Trp Ile Thr ggg Gly 170 ccg gat gcg cgg Pro Asp Ala Arg gat cga Asp Arg 180 agt aaa aga Ser Lys Arg aat ccc tcc Asn Pro Ser 200 gcg atc atc gtg Ala Ile Ile Val acc ggt act gcg Thr Gly Thr Ala ttg act gat Leu Thr Asp 195 gaa aat caa Glu Asn Gln ttg acg gcg cgg Leu Thr Ala Arg acc Thr 205 gat acg ggt ctt Asp Thr Gly Leu ccc agg Pro Arg 215 cgc gtt gtt att Arg Val Val Ile tcc cgc gag gtt Ser Arg Glu Val gca gat tcc aac Ala Asp Ser Asn gct cgc ttg gga Ala Arg Leu Gly tat 235 gag cag tac gcg Glu Gin Tyr Ala gga Gly 240 ata cca gag gct Ile Pro Glu Ala t ta Leu 245 tca gcg ctg tgg Ser Ala Leu Trp gat Asp 250 aaa ggg tgc cga Lys Gly Cys Arg gac Asp 255 att tta atc gaa Ile Leu Ile Glu ggt ggc Gly Gly 260 cca acg tta Pro Thr Leu cag gcc tat Gln Ala Tyr 280 ggg gca gcg ctg Gly Ala Ala Leu tta ggc att gtt Leu Gly Ile Val gat cag gtg Asp Gln Val 275 tca gtg att Ser Val Ile gtt gcc ccc gct Val Ala Pro Ala ttg ggc gct gga Leu Gly Ala Giy WO 01/00843 PCT/1B00100923 aac tgg cca caa gaa acc acg atg gat cag att atg cgt ttt gac acc 1027 Asn Trp Pro Gin Giu Thr Thr Met Asp Gin Ile Met Arg Phe Asp Thr 295 300 305 acg tcc gtg aga cag ttg ggt tca gat gta ttg ata gaa atg atg aga 1075 Thr Ser Val Arg Gin Leu Giy Ser Asp Val Leu Ilie Glu Met Met Arg 310 315 320 325 aag gaa cac taaatgttca caggtattgt cga 1107 Lys Giu His <210> 554 <211> 328 <212> PRT <213> Corynebacterium giutamicun <400> 554 met Asp Val Ala*His Ala Leu Asp Leu 1 Val Asp Giy Asn Arg Val Ala Ile His 145 Thr Val Thr 5 Thr Giu Giu Ala Cys Asn Giu Giu Lys Ser 165 Arg Asp Ser Val Val Val 70 Ser Ala Ala Pro Phe 150 Gin Ser Asn Asn Gly Aia Thr Ala Pro Val 120 Leu Ser Ile Arg Ser 200 Pro Val1 Leu Leu Leu Phe 105 Asp Val Thr Thr Asp 185 Leu Ala Pro Giy Aia diu Leu Pro Thr Ala Val1 Gly 170 Ala Thr His Val1 Ala Ala Pro Asp Ser His Thr Asp 155 Pro Ile Ala His Gly Thr Ala Cys Ala Ala Phe Arg 140 Gly Asp Ile Arg Val Ala Ala Gly Asn Gly Ala Leu 125 Leu Phe Ala Val1 Thr 205 Ser Val Pro Val His Ile Gly 110 Asp G ly Ala Arg Gly 190 Asp WO 01/00843 Leu Tyr Giu 210 Pro Ala Asp 225 Ilie Pro Giu Leu Ile Giu Ilie Val Asp 275 Giy Arg Ser 290 Met Arg Phe 305 Ile Giu Met Gin Asn Leu 245 Gly Val Ile Thr Arg 325 Pro Leu 230 Ser Pro Gin Asn Thr 310 Lys Vai Gly Asp 250 Gly Ala Giu Gin Ile Tyr 235 Lys Ala Pro Thr Leu 315 Arg Tyr Arg Arg 270 Leu Asp Asp PCTIBOOIOO923 Giu Val Ala Giy 240 Asp Ile 255 Leu Giy Giy Ala Gin Ile Vai Leu 320 <210> 555 <211> 1107 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (101)..(1084) <223> FRXA02246 <400> 555 tgccgacgct ggcgtggatg ccttcgttgc aggttccgct caacaaggcg atccaggagt tgcgagcact cgcgcagtaa gcg Ala agc Ser gtC Val1 gtg Vali gtg Val1 tta Leu cc t Pro gtt Val1 gtg Val1 gec Val gat Asp aat Asn ggC Giy gcg Ala acc Thr tca Ser gtc Val cc t Pro gtg Val cat Hius gat Asp att Ile cct Pro cgt Arg tac Tyr gtgtacggcg ctgaggatcc atg gat gtt gcg cac Met Asp Val Ala His 1 gtc cga ggc acc acc Val Arg Giy Thr Thr gac gcc gac ggc gag Asp Ala Asp Gly Giu ggc ccg cac gcc gaa Giy Pro His Ala Giu aac ggg ggc acg gcg Asn Gly Giy Thr Ala cgc acg ggt cca tgt Arg Thr Gly Pro Cys 115 163 211 259 307 355 WO 01/00843 PCT/IBOO/00923 tcc aag gcg ctt ctc gac gcc ggg atc gca cac gtg ttt tac gcc aat 403 Ser Lys Ala Leu Leu Asp Ala Gly Ile Ala His Val Phe Tyr Ala Asn 95 100 gcg gat ccc ttc ccg tca gcc gct ggg ggc ggt gcc ttt ttg gcg gag 451 Ala Asp Pro Phe Pro Ser Ala Ala Gly Gly Gly Ala Phe Leu Ala Glu 105 110 115 gcg ggc gtc gat acg cat ttt tta gat gag cgg atc agg gca ctg gag 499 Ala Gly Val Asp Thr His Phe Leu Asp Giu Arg Ile Arg Ala Leu Glu 120 125 130 ccc tgg ctg gtt gcg acg cgt ctg ggc agg ccc cat gtc acg ttg aag 547 Pro Trp Leu Val Ala Thr Arg Leu Gly Arg Pro His Val Thr Leu Lys 135 140 145 ttt gcg tcc acc gtg gac ggt ttt gct ggt gcc acc gat ggc acc agc 595 Phe Ala Ser Thr Val Asp Gly Phe Ala Gly Ala Thr Asp Gly Thr Ser 150 155 160 165 cag tgg att acc ggg ccg gat gcg cgg gcg ttt gtg cac gaa gat cga 643 Gin Trp Ile Thr Gly Pro Asp Ala Arg Ala Phe Val His Giu Asp Arg 170 175 180 agt aaa aga gat gcg atc atc gtg ggt acc ggt act gcg ttg act gat 691 Ser Lys Arg Asp Ala Ile Ile Val Gly Thr Gly Thr Ala Leu Thr Asp 185 190 195 aat ccc tcc ttg acg gcg cgg acc gat acg ggt ctt tat gaa aat caa 739 Asn Pro Ser Leu Thr Ala Arg Thr Asp Thr Gly Leu Tyr Giu Asn Gin 200 205 210 ccc agg cgc gtt gtt att ggc tcc cgc gag gtt cca gca gat tcc aac 787 Pro Arg Arg Val Val Ile Gly Ser Arg Giu Val Pro Ala Asp Ser Asn 215 220 225 ttg gct cgc ttg gga tat gag cag tac gcg gga ata cca gag gct tta 835 Leu Ala Arg Leu Gly Tyr Giu Gin Tyr Ala Gly Ile Pro Giu Ala Leu 230 235 240 245 tca gcg ctg tgg gat aaa ggg tgc cga gac att tta atc gaa ggt ggc 883 Ser Ala Leu Trp Asp Lys Gly Cys Arg Asp Ile Leu Ile Giu Giy Gly 250 255 260 cca acg tta gct ggg gca gcg ctg cgc tta ggc att gtt gat cag gtg 931 Pro Thr Leu Ala Gly Ala Ala Leu Arg Leu Giy Ile Val Asp Gin Val 265 270 275 cag gcc tat gtt gcc ccc gct ttg ttg ggc gct gga cga tca gtg att 979 Gin Ala Tyr Val Ala Pro Ala Leu Leu Gly Ala Gly Arg Ser Val Ile 280 285 290 aac tgg cca caa gaa acc acg atg gat cag att atg cgt ttt gac acc 1027 Asn Trp Pro Gin Giu Thr Thr Met Asp Gin Ile Met Arg Phe Asp Thr 295 300 305 acg tcc gtg aga cag ttg ggt tca gat gta ttg ata gaa atg atg aga 1075 Thr Ser Val Arg Gin Leu Gly Ser Asp Val Leu Ile Giu Met Met Arg WO 01/00843 PCTI/iBOO/00923 310 315 320 325 aag gaa cac taaatgttca caggtattgt cga 1107 Lys Glu His <210> 556 <211> 328 <212> PRT <213> Corynebacteriun glutamicum~ <400> 556 Met Asp Val Ala His Ala Leu Asp Leu Ala His His Val Ser Asp Gin 1 Val1 Asp Gly Asn Arg Val Ala Ile His 145 Thr Val Thr Leu Pro 225 Ile Arg Ala Pro Gly Thr Phe Phe Arg 130 Val Asp His Ala Tyr 210 Ala Pro Gly Asp His Gly Gly Tyr Leu 115 Al a Thr Gly Glu Leu 195 Glu Asp Glu Thr Gly Ala Thr Pro Ala 100 Ala Leu Leu Thr Asp 180 Thr Asn Ser Ala Pro Val1 Val 55 Val Lys Asp Gly Trp 135 Ala Trp Lys Pro Arg 215 Ala Ala Asn Gly 40 Ala Thr Ala Pro Val 120 Leu Ser Ile Arg Ser 200 Arg Arg Leu Pro Val Leu Leu Leu Phe 105 Asp Val Thr Thr Asp 185 Leu Val Leu Trp 10 Pro Gly Ala Glu Leu Pro Thr Ala Val Gly 170 Ala Thr Val Gly Asp 250 Val1 Al a Ala Pro 75 Asp Ser His Thr Asp 155 Pro Ile Ala Ile Tyr 235 Lys Gly Thr Ala Cys Ala Ala Phe Arg 140 Gly Asp Ile Arg Gly 220 Glu Gly Ala Ala Gly Asn Gly Ala Leu 125 Leu Phe Ala Val Thr 205 Ser Gin Cys Ile Pro Arg Tyr Ala Gly Glu Arg Gly Ala 175 Thr Thr Glu Ala Asp 255 Leu Gly Ala Gly His Gly Arg Pro Ala 160 Phe Gly Gly Val Gly 240 Ile WO 01/00843 WO 0100843PCTIIB0O/00923 Leu Ile Gly Met 305 Ile Pro Thr Leu Ala Gly Ala Ala Leu Arg 265 270 Gin Ala Tyr Val Ala Pro Ala Leu Leu 280 285 Asn Trp Pro Gin Giu Thr Thr Met Asp 295 300 Thr Ser Val Arg Gin Leu Gly Ser Asp 310 315 Lys Giu His <210> 557 <211> 756 <212> DNA <213> Corynebacterium giutamicun <220> <22i> CDS <222> (101)..(733) <223> RXA02247 <400> 557 acaagaaacc acgatggatc agattatgcg ttttgacacc acgtccgtga gacagttggg ttcagatgta ttgatagaaa tgatgagaaa ggaacactaa atg ttc aca.

Met Phe Thr ggt att Gly Ile gtc Val atc Ile ggg Giy ggc Gly agc Ser gcc Ala gtt Val tcC Ser tcC Ser gtc Vai act Thr tta Leu 75 ggc Giy tcg Ser gtt Vai gcg Ala aat Asn gca Ala tcc Ser cgt Arg ctg Leu ggc Gly acc Thr gtg Val ctc Leu ggt Gly ggt Gly aag Lys 110 gaa Giu tta Leu ttg Leu cag Gin aaa Lys cac His acc Thr 1 cat His gag Giu aca Thr gaa Giu gtc Val1 atc Ile agc Ser gga Gly gtg Val gcg Ala tta Leu ctt Leu caa Gin gag Giu 115 115 163 211 259 307 355 403 451 WO 01/00843 WO 0100843PCT/EBOO/00923 gat gtt ctg cgt ttt gag ctg cca gct gat ttg gct cgc tat gtg gtg 499 Asp Val Leu Arg Phe Glu Leu Pro Ala Asp Leu Ala Arg Tyr Val Val 120 125 130 gaa-aaa ggc tcc atc gca ctc aat ggc aca tcc ttg act gta tcg tct 547 Giu Lys Giy Ser Ile Ala Leu Asn Giy Thr Ser Leu Thr Val Ser Ser 135 140 145 ttg ggt gat gat tgg ttt gag gtt tcc ctg att ccc acc acc ttg cgc 595 Leu Giy Asp Asp Trp Phe Giu Val Ser Leu Ile Pro Thr Thr Leu Arg 150 155 160 165 gac acc acc cac ggc gaa ctg gcg gta ggg gat atc gta aac att gag 643 Asp Thr Thr His Gly Giu Leu Ala Val Gly Asp Ile Vai Asn Ile Giu 170 175 180 gtt gat gtg atc gct aag tac gtc gaa cgc atg atg acg cgc ggc gtg 691 Val Asp Val Ilie Ala Lys Tyr Val Giu Arg Met Met Thr Arg Gly Vai 185 190 195 gct gga aac act ccc aat gac tac acc gat ttc acg aga gac 733 Ala Gly Asn Thr Pro Asn Asp Tyr Thr Asp Phe Thr Arg Asp 200 205 210 taggttagac aacgtgagtg aac 756 <210> 558 <211> 211 <212> PRT <213> Corynebacterium glutanicun <400> 558 Met Phe Thr Gly Ile Val Glu Glu Leu Gly Ser Val Ala Gly Val Giu 1 5 10 His Leu Gly Asp Ser Ilie Arg Met Gin Ile Ser Ala Ser Thr Val Leu 25 Giu Gly Val His Leu Gly Asp Ser Ile Ser Val Asn Gly Val Cys Leu 40 Thr Val Ala Ser Phe Giy Giu Gly His Phe Thr Ala Asp Leu Met Gin 55 Giu Thr Leu Asp Arg Ser Ser Leu Gly Ala Leu Ser Thr Gly Ser Lys 70 75 Val Asn Leu Giu Arg Ala Met Ala Ala Asp Gly Arg Leu Gly Giy His 90 Ilie Met Gin Gly His Val Asp Ala Thr Thr Ser Leu Ile Lys Arg Thr 100 105 110 Ser Ser Giu Asn Trp Asp Val Leu Arg Phe Giu Leu Pro Ala Asp Leu 115 120 125 Ala Arg Tyr Val Vai Giu Lys Gly Ser Ile Ala Leu Asn Gly Thr Ser 130 135 140 WO 01/00843 Leu Thr Val Ser Ser Leu Gly Asp Asp Trp Phe 145 150 155 Pro Thr Thr Leu Arg Asp Thr Thr His Gly Glu 165 170 Ile Val Asn Ile Giu Val Asp Val Ile Ala Lys 180 185 Met Thr Arg Gly Val Ala Gly Asn Thr Pro Asn 195 200 Thr Arg Asp 210 <210> 559 <211> 1389 <212> DNA <213> Corynebacteriui giutanicun <22 0> <221> CDS <222> (101)..(1366) <223> RXN02248 <400> 559 gatgtgatcg ctaagtacgt cgaacgcatg atgacgcgcg PCTIIBOO/00923 Giu Val Ser Leu Ile 160 Leu Ala Val Gly Asp 175 Tyr Val Glu Arg Met 190 Asp Tyr Thr Asp Phe 205 gcgtggctgg aaacactccc aatgactaca ccgatttcac gagagactag gttagacaac gtg agt gaa cat gag Val Ser Giu His Glu 115 cag Gin gct Ala gaa Giu gc t Ala gca Ala cag Gin cac His ggt Gly gac Asp atg Met gat Asp gcc Ala caa Gin gcc Ala atc Ile cgt Arg gat Asp ggC Gly gat Asp gtg Val gcc Ala tcC Ser ctt Leu gc t Ala tct gtt Ser Vai gtg gta Val Val gcc gaa Ala Giu 45 tcg gga Ser Gly gat ctg ASP Leu tac acc Tyr Thr aca gac Thr Asp 110 gaa Giu 15 gat Asp tta Leu tac Tyr cc t Pro gtg Vali cgc Arg 1 gcc Ala gaa Giu act Thr tgt Cys atg Met gt t Val cac His ggc acc aca ggc att tct gca Gly Thr Thr Gly Ilie Ser Ala 105 WO 01/00843 ctt gct gat Leu Ala Asp 120 gtt gtg cca Val Val Pro 135 cac acc gaa His Thr Giu 150 gca ggt gtt Ala Gly Val gct cgg gtt Ala Arg Val ctg atc tct Leu Ile Ser 200 ttg gtg gag Leu Val Glu 215 ttc aag gct Phe Lys Ala 230 gcc att gtt Ala Ile Val gtt cga. gtc Val Arg Val cgc tgc gac Arg Cys Asp 280 gaa gct ggt 1027 Giu Ala Gly 295 ggc att ggt 1075 Gly Ile Gly 310 ggt gcc gac 1123 Gly Ala Asp gcc cgc gaa 1171 cca Pro c tg Leu gca Ala atc Ile cct Pro 185 att Ile cgc Arg gtt Val gcc Ala cac His 265 tgt Cys cgg Arg ctg Leu acc Thr ttt gaa gcC Glu Ala cgt gct Arg Ala gct gtc Ala Val 155 tgc gaa Cys Giu 170 gag ctg Glu Leu gag cag Giu Gin cag gtg Gin Val ggt tac Gly Tyr 235 ggc gac Gly Asp 250.

tct gag Ser Glu gga cag Gly Gin gga gta Gly Val ctc gcc Leu Ala 315 gtc gat Val Asp 330 ggc acc gac Asp cgt Arg 140 gat Asp gtg Val cgc Arg ctc Leu gaa Giu 220 cgt Arg gtg Val tgC Cys cag Gin gtg Val1 300 aag Lys gcc Ala agc cgc Arg 125 gaa Gi u ttg Leu gt C Val cgc Arg at t Ile 205 act Thr tcc Ser gca Ala ttg Leu ctg Leu 285 gtg Val cta Leu aac Asn gcc acg Thr ggt Gly gc t Ala agt Ser t tc Phe 190 gag Glu gtg Val atc Ile tcc Ser act Thr 270 c ac His tac Tyr cgc Arg ctc Leu cag gat Asp 9gC Gly cgc Arg gaa Glu 175 tgc Cys tgg Trp ctg Leu atc Ile gac Asp 255 ggt Gly gag Giu atg Met gcc Ala gca Ala 335 at t ttc Phe gtc Val gct Ala 160 gag Giu gat Asp cgt Arg cc t Pro gat Asp 240 ggt Gly gat Asp tct Ser cgt Arg tac Tyr 320 ctt Leu c tc acc Thr ttg Leu 145 gca Ala gac Asp gag Giu cgc Arg ac c Thr 225 ggc Gly ggc Gly gtt Val ttg Leu ggg Gly 305 caa Gin ggt Gly tac PCT/EB00100923 gga cac 499 Gly His gct gga 547 Ala Gly cgc cca 595 Arg Pro 165 ggc atg 643 Gly Met 180 ctg aag 691 Leu Lys gaa att 739 Giu Ile ggc acg 787 Gly Thr ctt gtt 835 Leu Val 245 gtC ctg 883 Val Leu 260 tcc cgg 931 Ser Arg atc cag 979 Ile Gin gga cga Gly Arg gat gaa Asp Giu 325 gcc gat Ala Asp 340 ggt gtg WO 01/00843 Ala Arg Giu Phe Gly Thr Ser Ala 345 cgc tcg ctc aac ttg atc agc aac 1219 Arg Ser Leu Asn Leu Ile Ser Asn 360 365 gaa ggc cac ggc att tcc att. gcc 1267 Glu Gly His Gly Ile Ser Ilie Ala 375 380 gtt cat gaa gac aat gtt cga tac 1315 Val His Glu Asp Asn Val Arg Tyr 390 395 gga cat gac ctc cca gat gtc gca 1363 Gly His Asp Leu Pro Asp Val Ala 410 aac taaggagcac aacaatggct. aaa 1389 Asn Gin 350 Ile Leu Tyr aac cca gcc aag Asn Pro Ala Lys agc cga acc ccc Ser Arg Thr Pro 385 ctg aaa acc aag Leu Lys Thr Lys 400 ctg tgg gaa caa Leu Trp Giu Gin 415 Leu 355 gtg Val cc t Pro gac Asp cac His PCTIIBOO/00923 Giy Val gga ctt Gly Leu gtt gct.

Val Ala cgc atg Arg Met 405 cca gaa Pro Giu 420 <210> 560 <211> 422 <212> PRT <213> Corynebacterium giutamicun <400> 560 Vai Ser Glu His Giu Gin Ala His Ser 1 5 Ala Ile Ala Asp Ile Ala Ala Gly Lys Glu Asp Arg Glu Asn Glu Gly Asp Ile 40 Thr Pro Giu Leu Val Ala Phe Met Val 55 Cys Ala Pro Leu Thr Ala Lys Asp Ala 70 Met Thr Ala His Asn Gin Asp Ala Arg Val Asp Ala Asn Thr Gly Thr Thr Gly 100 105 His Thr Leu Arg Leu Leu Ala Asp Pro 115 120 Thr Arg Pro Gly His Val Val Pro Leu 130 135 Asp Val Ala Ser Leu Ala Ala Asp Arg 140 Ser Val Ala Ser Asp Tyr Thr Arg 125 Glu Glu Asp Leu Tyr Pro Val Arg Asp Gly Glu Asp Ala Ile Pro Thr Ala Phe Val1 WO 01/00843 WO 0100843PCTIiBOOIO0923 Leu Val Arg Ala Gly His Thr Giu Ala Ala Val Asp Leu Ala Arg Ala 145 150 155 160 Ala Giy Leu Arg Pro Ala Gly Val Ile Cys Glu Val Val Ser Glu Glu 165 170 175 Asp Pro Thr Gly Met Ala Arg Val Pro Giu Leu Arg Arg Phe Cys Asp 180 185 190 Giu His Asp Leu Lys Leu Ilie Ser Ile Giu Gin Leu Ile Glu Trp Arg 195 200 205 Arg Lys Asn Glu Ilie Leu Val Glu Arg Gin Val Giu Thr Val Leu Pro 210 215 220 Thr Asp Phe Gly Thr Phe Lys Ala Val Gly Tyr Arg Ser Ile Ile Asp 225 230 235 240 Gly Thr Giu Leu Val Ala Ile Val Ala Gly Asp Val Ala Ser Asp Gly 245 250 255 Gly Glu Asn Val Leu Val Arg Val His Ser Giu Cys Leu Thr Gly Asp 260 265 270 Val Phe Gly Ser Arg Arg Cys Asp Cys Giy Gin Gin Leu His Giu Ser 275 280 285 Leu Arg Leu Ile Gin Giu Ala Gly Arg Gly Val Val Vai Tyr Met Arg 290 295 300 Giy His Giu Gly Arg Giy Ile Gly Leu Leu Ala Lys Leu Arg Ala Tyr 305 310 315 320 Gin Leu Gin Asp Giu Gly Ala Asp Thr Val Asp Ala Asn Leu Aia Leu 325 330 335 Gly Leu Pro Ala Asp Ala Arg Glu Phe Giy Thr Ser Ala Gin Ile Leu 340 345 350 Tlyr Asp Leu Gly Val Arg Ser. Leu Asn Leu Ile Ser Asn Asn Pro Ala 355 360 365 Lys Lys Val Gly Leu Glu Gly His Gly Ilie Ser Ilie Ala Ser Arg Thr 370 375 380 Pro Ile Pro Val Ala Val His Glu Asp Asn Val Arg Tyr Leu Lys Thr 385 390 395 400 Lys Arg Asp Arg Met Gly His Asp Leu Pro Asp Vai Ala Leu Trp Giu 405 410 415 Gin Glu His Pro Glu Asn 420 <210> 561 <211> 1389 <212> DNA <213> Corynebacterium glutamicun WO 01/00843 <220> <221> CDS <222> (101)..(1366) <223> FRXA02248 <400> 561 gatgtgatcg ctaagtacgt cgaacgcatg atgacgcgcg PCTIIBOO/00923 gcgtggctgg aaacactccc aatgactaca ccgatttcac gagagactag gttagacaac gtg agt gaa cat gag Val Ser Giu His Giu cag Gin gct Ala gaa Giu gct Ala gca Ala cag Gin Gly ctt Leu gtt Val1 cac His 150 gca.

Ala gct Al a agc Ser aaa Lys atc Ile gtg Val gca Ala cgc Arg ggc Gly 105 cca Pro ctg Leu gca Ala atc Ile cct Pro 185 caa Gin gc c Ala atc Ile cgt Arg gat Asp ggC Gly att Ile gaa Giu cg t Arg gc t Ala tgc Cys 170 gag Giu tta Leu gtc Val ttt Phe tat Tyr cgt Arg acc Thr t Ct Ser gcc Ala gct Ala gtc Val1 155 gaa Giu ctg Leu tc t Ser gtg Val gcc Ala tcg Ser gat Asp tac Tyr aca Thr cgc Arg i2 5 gaa Giu ttg Leu gtc Vai cgc Arg gtt Vai gta Val gaa Glu gga Gly ctg Leu acc Thr gac Asp 110 acg Thr ggt Giy gc t Aia ag t Ser t tc Phe 190 gaa Glu i5 gat Asp tta Leu tac Tyr cct Pro gtg Val cgc Arg ga t Asp ggc Gly cgc Arg gaa Giu 175 tgc Cys 1 gcc Ala gaa Glu act Thr tgt Cys atg Met gtt Val cac His ac C Thr ttg Leu 145 gca Ala gac Asp gag Giu atc Ile ga t Asp cca Pro gcg Ala acc Thr gat Asp act Thr cgt Arg 130 gtg Val ggc Gly ccc Pro c ac His ctg atc tct att gag cag ctc att gag tgg cgt cgc aag aat gaa att WO 01/00843 Leu Ile Ser 200 ttg gtg gag Leu Val Giu 215 ttc aag gct Phe Lys Ala 230 gcc att gtt Ala Ile Val gtt cga gtc Val Arg Val cgc tgc gac Arg Cys Asp 280 gaa gct ggt 1027 Giu Ala Gly 295 ggc att ggt 1075 Gly Ile Gly 310 ggt gcc gac 1123 Gly Ala Asp gcc cgc gaa 1171 Ala Arg Giu cgc tcg ctc 1219 Arg Ser Leu 360 gaa ggc cac 1267 Glu Gly His 375 gtt cat gaa 1315 Val His Glu 390 gga cat gac 1363 Ile cgc Arg gtt Val gcc Ala cac His 265 tgt Cys cgg Arg ctg Leu acc Thr ttt Phe 345 aac Asn ggc Gly gac Asp C tC Glu cag Gin ggt Gly ggc Gly 250 tct Ser gga Gly gga Gly ctc Leu gtc Val 330 ggc Gly ttg Leu att Ile aat Asn cca Giu gtg Val atc Ile tcC Ser act Thr 270 cac His tac Tyr cgc Arg ctc Leu cag Gin 350 aac Asn agc Ser ctg Leu ctg Trp ctg Leu atc Ile gac Asp 255 ggt Gly gag Giu atg Met gcc Ala gca Ala 335 att Ile cca Pro cga Arg aaa Lys tgg Arg cc t Pro gat Asp 240 ggt Gly gat Asp tct Ser cgt Arg tac Tyr 320 ctt Leu ctc Leu gcc Ala acc Thr acc Thr 400 gaa Arg acc Thr 225 99c Gly ggc Gly gtt Val t tg Leu 9gg Gly 305 caa Gin ggt Gly tac Tyr aag Lys ccc Pro 385 aag Lys caa Gin PCTIIBOO/00923 Giu Ile ggc acg 787 Gly Thr ctt gtt 835 Leu Val 245 gtc ctg 883 Vai Leu 260 tcc cgg 931 Ser Arg atc cag 979 Ile Gin gga cga Gly Arg gat gaa Asp Giu 325 gcc gat Ala Asp 340 ggt gtg Gly Val gga ctt Gly Leu gtt gdt Val Ala cgc atg Arg Met 405 cca gaa Pro Giu Gly His Asp Leu Pro Asp Val Ala Leu Trp Giu WO 01/00843 WO 0100843PCT/EBOO/00923 410 415 420 aac taaggagcac aacaatggct aaa 1389 Asn <210> 562 <211> 422 <212> PRT <213> Corynebacterium giutamicun <400> 562 Val Ser Giu His Giu Gin Ala His Ser Gin Leu Asp Ser Val Giu Glu 1 5 10 Ala Ilie Ala Asp Ile Ala Ala Gly Lys Ala Val Val Val Val Asp Asp 25 Giu Asp Arg Giu Asn Giu Gly Asp Ile Ile Phe Ala Ala Giu Leu Ala 40 Thr Pro Giu Leu Val Ala Phe Met Val Arg Tyr Ser Ser Giy Tyr Ile 55 Cys Ala Pro Leu Thr Ala Lys Asp Ala Asp Arg Leu Asp Leu Pro Pro 70 75 Met Thr Ala His Asn Gin Asp Ala Arg Gly Thr Ala Tyr Thr Val Thr 90 Val Asp Ala Asn Thr Gly Thr Thr Gly Ilie Ser Ala Thr Asp Arg Ala 100 105 110 His Thr Leu Arg Leu Leu Ala Asp Pro Giu Ala Asp Arg Thr Asp Phe 115 120 125 Thr Arg Pro Gly His Val Val Pro Leu Arg Ala Arg Giu Gly Gly Val 130 135 140 Leu Vai Arg Ala Gly His Thr Giu Ala Ala Val Asp Leu Ala Arg Ala 145 150 155 160 Ala Gly Leu Arg Pro Ala Gly Vai Ile Cys Glu Val Val Ser Glu Glu 165 170 175 Asp Pro Thr Gly Met Ala Arg Val Pro Giu Leu Arg Arg Phe Cys Asp 180 185 190 Glu His Asp Leu Lys Leu Ile Ser Ile Giu Gin Leu Ile Glu Trp Arg 195 200 205 Arg Lys Asn Giu Ile Leu Val Giu Arg Gin Val Glu Thr Val Leu Pro 210 215 220 Thr Asp Phe Gly Thr Phe Lys Ala Val Gly Tyr Arg Ser Ile Ile Asp 225 230 235 240 Gly Thr Giu Leu Val Ala Ile Val Ala Gly Asp Val Ala Ser Asp Gly 245 250 255 WO 01/00843 WO 0100843PCT/IBOO/00923 Gly Vai Leu Gly 305 Gin Gly Tyr Lys Pro 385 Lys Gin Val1 260 Ser Ile Gly Asp Ala 340 Gly Gly Val1 Arg Pro 420 Val Arg Glu Gly 310 Gly Ala Arg Giu Val 390 Gly Asn Ser Gly Gly Leu Val 330 G1y Leu Ile Asn Pro 410 Thr 270 His Tyr Arg Leu Gin 350 Asn Ser Leu Leu Gly Glu Met Al a Ala 335 Ile Pro Arg Lys Trp 415 <210> 563 <211> 600 <212> DNA <213> Corynebacteriui giutamicum <22 0> <221> CDS <222> (101)..(577) <223> RXN02249 <400> 563 atgttcgata cctgaaaacc aagcgtgacc gcatgggaca tgtgggaaca agagcaccca gaaaactaag gagcacaaca ttg ccg gca gtc gaa ctc ccc gac gcc agc gga Leu Pro Ala Val Glu Leu Pro Asp Ala Ser Gly 15 gtc acc gca cgg tgg aac gca gaa atc tgc gac Val Thr Ala Arg Trp Asn Ala Glu Ilie Cys Asp 30 gca gta gat gcg gga cgt gca gca gga gca acg 807 tgacctccca gatgtcgcac atg gct aaa gaa gga Met Ala Lys Glu Gly 1 tta aaa gtc gcc gta Leu Lys Val Ala Val cgc ctg cac aag cac Arg Leu His Lys His gtg agc gaa tac cgc WO 01/00843 Ala Val Asp gtc atc ggc Val Ile Gly acc cat gac Thr His Asp cca cac ttt Pro His Phe att gct ctt Ile Ala Leu aac acc gaa Asn Thr Giu 120 gag gac aaa Glu Asp Lys 135 Gly ctg Leu gta Val tac Tyr act Thr caa Gin gca Ala Arg gaa Glu gtt Val 75 gtg Val tcc Ser gcc Ala gag Glu Val1 caa Gin gt t Val1 gaa Giu ggt Giy gga Gly ctc Leu 145 PCTIIBOO/00923 Tyr Arg gca cgc 307 Ala Arg ggc acc 355 Giy Thr acc cgc 403 Thr Arg 100 act acc 451 Thr Thr tct gta 499 Ser Vai gcc ctc 547 Ala Leu ctt tct caa att cgt Leu Ser Gin Ile Arg 155 gca act gag ggt Ala Thr Glu Giy tagactgttc tttaaaaggt ttg <210> 564 <211> 159 <212> PRT <213> Corynebacteriun giutaiicun <400> 564 Met Ala Lys Giu Gly Leu Pro Ala Val 1 Leu Lys Vai Ala Vai Vai Thr Ala Arg 25 Arg Leu His Lys His Ala Val Asp Ala 40 Val Ser Giu Tyr Arg Val Ile Gly Ala Gin Giu Leu Ala Arg Thr His Asp Ala Val Arg Gly Gly Thr Pro His Phe Asp Glu Gly Leu Thr Arg Ile Ala Leu Asp 100 105 Asp Glu Ala Pro Leu Asp Pro Al a Ile Gly Val1 Gly Ser Ile 110 WO 01/00843 PCTIBOO/00923 Gly Val Leu Thr Thr Asn Thr Giu Giu Gin Ala Val Giu Arg Ser Gly 115 120 125 Gly Giu Gly Ser Val Giu Asp Lys Gly Ala Glu Ala Met Val Ala Ala 130 135 140 Leu Asp Thr Ala Leu Val Leu Ser Gin Ile Arg Ala Thr Giu Gly.

145 150 155 <210> 565 <211> 600 <212> DNA <213> Corynebacteriui glutamicun <22 0> <221> CDS <222> (101)..(577) <223> FRXA02249 <400> 565 atgttcgata cctgaaaacc aagcgtgacc gcatgggaca tgacctccca gatgtcgcac tgtgggaaca agagcaccca gaaaactaag gagcacaaca atg gct aaa gaa gga Met Ala Lys Glu Gly ttg Leu gtc Val gca Ala gtc Val1 acc Thr cca Pro at t Ile aa c Asn gag ccg Pro acc Thr gta Val atc Ile cat His cac His gc t Ala acc Thr gac gaa ctc Glu Leu tgg aac Trp Asn gga cgt Gly Arg ctg gaa Leu Glu gta gtt Val Val 75 tac gtg Tyr Val act tcc Thr Ser caa gcc Gin -Ala gca gag ccc Pro gca Ala gca Ala ctt Leu 60 gcc Ala tgc Cys acc Thr gtg Val gca gcc Ala atc Ile gga Gly gtc Val ggc Gly tct Ser atc Ile 110 cgc Arg gtc gga Gly gac Asp acg.

Thr gtg Val gtc Val acc Thr aac Asn ggt Gly 1 tta Leu cgc Arg gtg Val1 caa Gin gtt Val1 gaa Glu ggt Gly gga Gly aaa Lys ctg Leu agc Ser gaa Giu cgt Arg ggc Gly gtg Val1 gaa Glu 130 gtc Val cac His gaa Glu ctg Leu 9gc Gly c tc Leu ttg Leu 115 ggc Gly 115 163 211 259 307 355 403 451 499 547 gct gca ctc gat act gcc ctc Glu Asp Lys Gly Ala Giu Ala Met Val Ala Ala Leu Asp Thr Ala Leu WO 01/00843 PCTJIBO0/00923 135 140 145 gtg Ctt tct caa att cgt gca act gag ggt tagactgttc tttaaaaggt 597 Val Leu Ser Gin Ile Arg Ala Thr Glu Giy 150 155 ttg 600 <210> 566 <211> 159 <212> PRT <213> Corynebacteriui giutarnicum <400> 566 Met Ala Lys Giu 1 Leu Lys Vi Arg Leu H: Val Ser G' Gin Glu Lq Val Arg G' Glu Giy Li Giy Val Li 1: Gly Glu G' 130 Leu Asp T1 145 <210> 567 <211> 702 <212> DNA Ala Lys Tyr Ala Giy Thr 100 Thr S er Ala Gly Val1 His Arg Arg Thr Arg Thr Val Leu Leu Pro Ala Val Pro Aia Ala Leu Ala Cys Thr Val Ala 140 Ala Asp Glu Ala Pro Leu Asp Pro Giu 125 Met Thr <213> Corynebacteriun glutamicum <2 <221> CDS <222> (101)..(679) <223> RXA02250 <400> 567 caactgaggg ttagactgtt ctttaaaagg tttgacgcct cggtttaatg cctagcagga tgcgcgccac cgctaattag ttctaaggat ttgtgaaatc gtg aca acc aac gcc 115 Val Thr Thr Asn Ala WO 01/00843 WO 0100843PCTIIBOO/00923 ccc gac gg4 gca Pro Asp Gly Ala aac aac atc Asn Asn Ile aac aac gca cat tcg ggc Asn Asn Ala His Ser Gly gct gtc Ala Val ggg aag cca Gly Lys Pro gca gct ttc Ala Ala Phe aag Lys gta cag ctc agc Val Gin Leu Ser gcg gaa att cag gaa tac acc Ala Giu Ile Gin Giu Tyr Thr gct ggc acc acc aca acc aag cca tgg Ala Gly Thr Thr Thr Thr Lys Pro Trp ctg gag gtg Leu Glu Val acc aca Thr Thr aag ttt ctg aaa Lys Phe Leu Lys atc gcg tgg gta Ile Ala Trp Val gcc Ala gtg gtt gtc atc Val Val Val Ile atg Met gcc gtt cac atc Ala Val His Ile atg ggt gcc gtg Met Gly Ala Val gtg Val gac gtc gat ttc Asp Val Asp Phe ggt gca gcg gtc Gly Ala Ala Val ttt gtt gac act Phe Val Asp Thr gca ttc cca gcg Ala Phe Pro Ala ttg ggc Leu Gly 100 atc atc ttc Ile Ile Phe cgt gcc aac Arg Ala Asn 120 t cc Ser 105 gtt ctt gtg ttc ttg gga ctg act cgc Val Leu Val Phe Leu Gly Leu Thr Arg 110 cct cgc gtg Pro Arg Val 115 gga act cgt Gly Thr Arg gaa gac ggc gtt Glu Asp Gly Val gtg cgt aac ttc Val Arg Asn Phe atc Ile 130 499 ttc tac Phe Tyr 135 cca tgg gtt gtc atc tac ggc atg tct ttc ccc aag ggc agc Pro Trp Val Val Ile Tyr Gly Met Ser Phe Pro Lys Gly Ser 140 145 gtg gca cgt ttg Val Ala Arg Leu ctt cca gac ttt Leu Pro Asp Phe gaa Glu 160 ttc gtt ccc atg Phe Val Pro Met gct ttc cag tcc Ala Phe Gin Ser gat gga gaa gat Asp Gly Glu Asp gtg cgc gcg gtt Val Arg Ala Val gcg acc Ala Thr 180 ttc cgc gac ctc gaa aac aag tac Phe Arg Asp Leu Giu Asn Lys Tyr 185 atg cca gag gac Met Pro Glu Asp 190 taattaagct gtggctgatc caa <210> 568 <211> 193 <212> PRT <213> Corynebacteriun glutamicum <400> 568 Val Thr Thr Asn Ala Pro Asp Gly Ala 1 5 Thr Asn Asn Ile Asn Asn Ala 10 WO 01/00843 WO 0100843PCTJIBOO/00923 His Ser Gly Ala Val Gly Lys Pro Lys Val Gin Leu Ser Asp Ala Giu 25 Ile Gin Giu Tyr Thr Ala Ala Phe Ala Gly Thr Thr Thi- Thi- Lys Pro 40 Ti-p Giu Leu Giu Val Thi- Thr Lys Phe Leu Lys Lys Ile Ala Ti-p Val 55 Ala Val Val Val Ile Met Ala Val His Ile Phe Met Gly Ala Val Val 70 75 Asp Val Asp Phe Thr Gly Ala Ala Val Thr Phe Val Asp Thr Leu Ala 90 Phe Pro Ala Leu Gly Ile Ile Phe Ser Val Leu Val Phe Leu Gly Leu 100 105 110 Thr Arg Pro Arg Val Arg Ala Asn Giu Asp Gly Val Giu Val Arg Asn 115 120 125 Phe Ile Gly Thr Arg Phe Tyr Pro Ti-p Val Vai Ile Tyr Gly Met Ser 130 135 140 Phe Pro Lys Giy Ser Ser Vai Ala Arg Leu Giu Leu Pro Asp Phe Giu 145 150 155 160 Phe Val Pro Met T-p, Ala Phe Gin Ser Arg Asp Giy Giu Asp Val Val 165 170 175 Arg Ala Val Ala Thi- Phe Arg Asp Leu Giu Asn Lys Tyr Met Pro Giu 180 185 190 Asp <210> 569 <211> 1146 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (101). .(1123) <223> RXA01489 <400> 569 gtcatgggat gtcattccgg cgggcttgtc gacgatcacg agtccaggtt taggggcagg agcattcatg tctgttgagt ctatgccgta gtctaaaaca gtg gat att tgg agt 115 Val Asp Ile Ti-p Ser 1 gga cta gac agc gtt ccg gct gat ctt caa gga tca gta gtc acc att 163 Gly Leu Asp Ser Val Pro Ala Asp Leu Gin Gly Ser Val Val Thr Ile 15 ggt gtg ttt gat ggg ctc cac cgg ggg cat caa agt tta atc ggc gag 211 Gly Val Phe Asp Gly Leu His Arg Gly His Gin Ser Leu Ile Gly Glu 812 WO 01/00843 WO 0100843PCTIIBOO/00923 atg gtg acc Met Val Thr gcc aag aag Ala Lys Lys cag gcc gag gag Gin Ala Giu Giu ggt gtg cct tgt Gly Val Pro Cys gt c Val ttt gac Phe Asp ccg cat ccg atc Pro His Pro Ile gtg ttt ttg cca Val Phe Leu Pro aaa gag cca acc Lys Glu Pro Thr cgt Arg ttg gct cct ttg Leu Ala Pro Leu tat cgc ctt aat Tyr Arg Leu Asn gct gcg gaa tgt Ala Ala Glu Cys gtc gat gct gcg Val Asp Ala Ala ttg Leu gtt att gat ttc Val Ile Asp Phe aaa gaa ctc gca Lys Glu Leu Ala ggt ctg Gly Leu 100 agc gct gaa Ser Ala Glu cgt tca gtt Arg Ser Val 120 tat ttc aca acc Tyr Phe Thr Thr atc gtg gat acg ctg cat gcg Ile Val Asp Thr Leu His Ala 115 gtg gtg ggg gag Val Val Gly Glu ttc acc ttc ggt Phe Thr Phe Gly gtc Val1 130 aat ggc gct Asn Gly Ala ggc act Gly Thr 135 gag tcc acg atg Glu Ser Thr Met gaa ttg gga caa Giu Leu Gly Gin ttt ggc gtg aat Phe Gly Val Asn gt c Vai 150 acg att gct ccg ctg ctg cat gat gat Thr Ile Ala Pro Leu Leu His Asp Asp 155 cag cgt att tgc Gin Arg Ile Cys acc ttg gtg cgc Thr Leu Val Arg gat Asp 170 tac ttg gat cag Tyr Leu Asp Gin gag gtt gag cgc Glu Val Giu Arg gcg aac Ala Asn 180 tgg gcg ctt Trp Ala Leu gct ggc cgt Ala Gly Arg 200 cga cgc tat gcc Arg Arg Tyr Ala cgc ggc gaa gtt Arg Giy Glu Val gtc cgt ggt Val Arg Gly 195 aat ctc tac Asn Leu Tyr ggc ggc aaa gaa Gly Gly Lys Glu ggc tat ccc acc Gly Tyr Pro Thr gcg Ala 210 ctg ccg Leu Pro 215 acc tct gtg gcg Thr Ser Val Ala ccc gcc gat ggc Pro Ala Asp Gly tat gca ggc tgg Tyr Ala Gly Trp acc atc acc gat Thr Ilie Thr Asp gac Asp 235 cgc gaa atc gac Arg Glu Ile Asp gaa atc tcc cgc Giu Ile Ser Arg atc gac ggc acc Ile Asp Gly Thr atg Met 250 gtt cca ggc gtg Val Pro Gly Val cgt tac caa act gcc att tcc Arg Tyr Gin Thr Ala Ile Ser 255 260 gtg ggc acc Val Gly Thr ccc acc ttc ggc Pro Thr Phe Gly gag cga cgc agc Giu Arg Arg Ser gtc gag gca Vai Giu Ala 275 WO 01/00843 WO 0100843PCT/EBOO/00923 ttc atc ctc gac cag gaa gcc gac ctg tac ggt cac cat gtc atg gtg 979 Phe Ilie Leu Asp Gin Giu Ala Asp Leu Tyr Gly His His Val Met Val 280 285 290 gaa ttc gtg gga cac ttg cgc gac atg gtc aaa ttc aac ggc gtc gac 1027 Giu Phe Val Gly His Leu Arg Asp Met Val Lys Phe Asn Gly Val Asp 295 300 305 gag cta cta gac gcc atg gcc cga gat gtc acc aac gcc cgc gac atc 1075 Glu Leu Leu Asp Ala Met Ala Arg Asp Val Thr Asn Ala Arg Asp Ile 310 315 320 325 ctt gcc aaa gac aaa ttg ctt ctc gac gcc gac acc cag ccc agc gct 1123 Leu Ala Lys Asp Lys Leu Leu Leu Asp Ala Asp Thr Gin Pro Ser Ala 330 335 340 taaggccggt caccggccat caa 1146 <210> 570 <211> 341 <212> PRT <213> Corynebacterium giutamicun <400> 570 Val Asp Ile Trp Ser Giy Leu Asp Ser Val Pro Ala Asp Leu Gin Gly 1 5 10 Ser Vai Vai Thr Ile Giy Val Phe Asp Giy Leu His Arg Giy His Gin 25 Ser Leu Ilie Gly Glu Ala Lys Lys Gin Ala Giu Giu Leu Giy Val Pro 40 Cys Val Met Val Thr Phe Asp Pro His Pro Ile Ala Val Phe Leu Pro 55 Gly Lys Glu Pro Thr Arg Leu Ala Pro Leu Asp Tyr Arg Leu Asn Leu 70 75 Ala Ala Giu Cys Gly Val Asp Ala Ala Leu Val Ile Asp Phe Thr Lys 90 Glu Leu Ala Gly Leu Ser Ala Glu Glu Tyr Phe Thr Thr Met Ile Val 100 105 110 Asp Thr Leu His Ala Arg Ser Val Val Val Gly Glu Asn Phe Thr Phe 115 120 125 Gly Val Asn Gly Ala Giy Thr Giu Ser Thr Met Arg Glu Leu Gly Gin 130 135 140 Lys Phe Gly Val Asn Val Thr Ile Ala Pro Leu Leu His Asp Asp Asp 145 150 155 160 Gin Arg Ilie Cys Ser Thr Leu Val Arg Asp Tyr Leu Asp Gin Gly Giu WO 01/00843 WO 0100843PCTJIBOO/00923 165 170 175 Val Glu Arg Ala Asn Ti-p Ala Leu Gly Arg Arg Tyr Ala Val Arg Gly 180 185 190 Giu Val Val Arg Gly Ala Gly Arg Gly Gly Lys Giu Leu Gly Tyr Pro 195 200 205 Thr Ala Asn Leu Tyr Leu Pro Thr Ser Val Ala Leu Pro Ala Asp Gly 210 215 220 Val Tyr Ala Gly Ti-p Phe Thr Ile Thr Asp Asp Arg Giu Ile Asp Lys 225 230 235 240 Glu Ile Ser Arg Asp Ilie Asp Gly Thr Met Val Pro Gly Val Arg Tyr 245 250 255 Gin Thr Ala Ile Ser Vai Gly Thr Asn Pro Thr Phe Gly Asp Glu Arg 260 265 270 Arg Ser Val Giu Ala Phe Ile Leu Asp Gin Glu Ala Asp Leu Tyr Gly 275 280 285 His His Val Met Val Glu Phe Val Gly His Leu Arg Asp Met Val Lys 290 295 300 Phe Asn Gly Val Asp Glu Leu Leu Asp Ala Met Ala Arg Asp Vai Thi- 305 310 315 320 Asn Ala Arg Asp Ile Leu Ala Lys Asp Lys Leu Leu Leu Asp Ala Asp 325 330 335 Thr Gin Pro Ser Ala 340 <210> 571 <211> 1197 <212> DNA <213> Corynebacterium giutamicun <22 0> <221> CDS <222> (101)..(1174) <223> RXA02135 <400> 571 cgtgtcgcag cgatttgcga gagggttgtc ttcgtggttg ctggtctgcc actagagttg aaaacgtttt aagaaaacag tcggtttgaa ggagttgtta atg gtt cca gca gag 115 Met Val Pro Ala Glu 1 ctt ttt gcg cgt gtg gaa ttt ccg gat cat aaa atc ctg gct cag acg 163 Leu Phe Ala Arg Val Glu Phe Pro Asp His Lys Ilie Leu Ala Gin Thr 15 aag gat ttc cat gac tcc ctc acc aag cca ccc gga tct ttg ggc aag 211 Lys Asp Phe His Asp Ser Leu Thr Lys Pro Pro Gly Ser Leu Gly Lys 30 WO 01/00843 ttg gag cag atc ggc tgt ttc att tcc gca tgc Leu Giu Gin Ile Gly Cys Phe Ile Ser Ala Cys 45 cca Pro ggc Gly cag Gin gca Ala cac His gat Asp atc Ile 150 tta Leu ctc Leu ggC Gly cgc Arg atc Ilie 230 att Ile gta Vali cgt Arg gtt Vai atg Met cgc Arg gaa Glu gtt Vai 135 ggt Giy atc Ile gt t Val1 ac c Thr gac Asp 215 gcc Ala gcc 'Aia gtc Val aa c Asn aaa Lys aac Asn ggc Gly ggC Gly gcc Ala att Ile gat Asp 170 ttc Phe gac Asp t tc Phe atc Ile gca Ala 250 gca Ala aac Asn ggC Giy 75 att Ile acg Thr gac Asp atg Met gcg Ala 155 tta Leu acc Thr gat Asp cgc Arg tct Ser 235 gtt Val1 gcc Ala tca Ser 60 gtg Val aca Thr t cc Ser gag Glu acc Thr 140 gat Asp gga Gly ctc Leu gaa Giu gcc Ala 220 tcc Ser cga Arg ctc Leu PCTIBOO/00923 cag ggc cag att ccg 259 Gin Gly Gin Ile Pro ttc gct ggc gat cac 307 Phe Ala Gly Asp His tcc tca gta agc ttg 355 Ser Ser Val Ser Leu gcc atc aac gtg att 403 Ala Ile Asn Vai Ile 100 gat acc tcc ctc gac 451 Asp Thr Ser Leu Asp 115 tcc tgc gga tcc atc 499 Ser Cys Giy Ser Ile 130 gaa cgc gr-a ctg aag 547 Giu Arg Ala Leu Lys 145 gca ggc gcc gac att 595 Ala Gly Ala Asp Ile 165 acc acc gcc gct gcc 643 Thr Thr Ala Ala Ala 180 gtt gtc gta ggc cgc 691 Val Val Val Gly Arg 195 aaa gtc tcc gcg atc 739 Lys Vai Ser Ala Ile 210 caa gac ccc atc gcc 787 Gin Asp Pro Ile Ala 225 gcc atg gca gca ttc 835 Ala Met Ala Ala Phe 245 ctt ctc gac ggc gtt 883 Leu Leu Asp Gly Val 260 ctg gcc cca ggt gcc 931 Leu Ala Pro Gly Ala 275 gaa cca gcg cat tcc 979 agg cgt tgg ttc atc gca gga cac cgc tcc acc WO 01/00843 Arg Arg Trp Phe Ile Ala Gly His 280 285 gta gct cta aac gca ctg gcc ctt 1027 Val Ala Leu Asn Ala Leu Ala Leu 295 300 tcc ctt ggc gaa ggc tcc ggc gca 1075 Ser Leu Gly Giu Gly Ser Gly Ala 310 315 att gcc gtt gac ctg atg aac gac 1123 Ilie Ala Val Asp Leu Met Asn Asp 330 gtc gat gga ccc cta aac gcc tct 1171 Val Asp Gly Pro Leu Asn Ala Ser 345 gag taactttcta agcgatgtcc ggc 1197 Glu 3 <210> 572 <211> 358 <212> PRT <213> Corynebacterium giutamicun <400> 572 Met Val Pro Ala Giu Leu Phe Ala I 1 5 Ilie Leu Ala Gin Thr Lys Asp Phe 1 Gly Ser Leu Gly Lys Leu Glu Gin Gin Gly Gin Ile Pro Pro Arg Pro I 55 Phe Ala Gly Asp His Gly Val Ala 'J 70 Ser Ser Val Ser Leu Gin Met Ala C Ala Ile Asn Val Ile Ala Arg Thr I] 1001 Asp Thr Ser Leu Asp His Giu Ala 115 120 Ser Cys Gly Ser Ilie Asp Val Giu 130 135 rg ~at ~sp ;cc l1a Ltg ~et :cc er ~50 ~rg is5 25 le ~eu rhr ;lu hr .05 ~rp ~sp Ser ccc Pro acc Thr t cg Ser 335 gaa Giu Val Asp Gly Asn Lys Asn Gly Gly Ala Giu ctg Leu 305 ctc Leu ttt Phe ccc Pro Phe Leu Phe Ser Val1 Thr Ser Giu Thr 140 Ala ctt Leu ctg Leu tcc Ser caa Gin 355 Asp Lys Ser Ile Ala Gly Arg 110 Val Glu PCTIBOO/00923 His Ser gga atg Gly Met gtc aag Val Lys 325 gcc ggc Ala Gly 340 aac acg Asn Thr Lys Pro Cys Val Pro Ala Ile Arg Val WO 01/00843 PCT/IB00/00923 Glu Arg Ala Leu Lys Ile Gly Lys Arg Ile Ala Asp Gin Glu Val Asp 145 150 155 160 Ala Gly Ala Asp Ile Leu Ile Pro Gly Asp Leu Gly Ile Gly Asn Thr 165 170 175 Thr Thr Ala Ala Ala Leu Val Gly Thr Phe Thr Leu Ala Glu Pro Val 180 185 190 Val Val Val Gly Arg Gly Thr Gly Ile Asp Asp Glu Ala Trp Lys Leu 195 200 205 Lys Val Ser Ala Ile Arg Asp Ala Met Phe Arg Ala Arg Asp Leu Arg 210 215 220 Gin Asp Pro Ile Ala Ile Ala Arg Lys Ile Ser Ser Pro Asp Leu Ala 225 230 235 240 Ala Met Ala Ala Phe Ile Ala Gin Ala Ala Val Arg Arg Thr Pro Val 245 250 255 Leu Leu Asp Gly Val Val Val Thr Ala Ala Ala Leu Leu Ala Asn Lys 260 265 270 Leu Ala Pro Gly Ala Arg Arg Trp Phe Ile Ala Gly His Arg Ser Thr 275 280 285 Glu Pro Ala His Ser Val Ala Leu Asn Ala Leu Ala Leu Asp Pro Ile 290 295 300 Leu Glu Leu Gly Met Ser Leu Gly Glu Gly Ser Gly Ala Ala Thr Ala 305 310 315 320 Leu Pro Leu Val Lys Ile Ala Val Asp Leu Met Asn Asp Met Ser Thr 325 330 335 Phe Ser Ser Ala Gly Val Asp Gly Pro Leu Asn Ala Ser Ser Glu Ala 340 345 350 Pro Glu Gin Asn Thr Glu 355 <210> 573 <211> 1146 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1123) <223> RXA01489 <400> 573 gtcatgggat gtcattccgg cgggcttgtc gacgatcacg agtccaggtt taggggcagg agcattcatg tctgttgagt ctatgccgta gtctaaaaca gtg gat att tgg agt 115 Val Asp Ile Trp Ser 1 WO 01/00843 .gga cta gac agc gtt ccg gct gat ctt caa gga Gly Leu Asp Ser Val Pro Ala Asp Leu Gin Gly 15 PCTJ/iBOOI00923 tca gta gtc Ser Val Val ggt Gly gcc Ala ttt Phe cgt Arg gtc Val1 agc Ser cgt Arg ggc Gly gtc Val 150 ac c Thr tgg Trp gc t Ala ctg Leu ttc Phe 230 gtg Val aag Lys gac Asp t tg Leu gat Asp gct Ala tca Ser act Thr 135 acg Thr t tg Leu gcg Ala ggc Gly ccg Pro 215 acc Thr ttt Phe aag Lys ccg Pro gct Ala gc t Ala gaa Giu gt t Val 120 gag Giu at t Ile gtg Val ctt Leu cgt Arg 200 acc Thr atc Ile ctc Leu gag Giu atc Ilie gat Asp 75 gt t Val1 ttc Phe ggg Gly atg Met c tg Leu 155 t ac Tyr cgc Arg aaa Lys gcg Ala gac Asp 235 cac His gag Glu gc t Ala tat Tyr att Ile aca Thr gag Giu Arg 140 ctg Leu t tg Leu tat Tyr gaa Glu ctg Leu 220 cgc Arg cgg Arg ctg Leu gtg Val cgc Arg gat Asp ac c Thr aac Asn 125 gaa Giu cat His gat Asp gcc Al a ttg Leu 205 ccc Pro gaa Glu ggg Gly ggt Gly ttt Phe ct t Leu ttc Phe atg Met 110 ttc Phe ttg Leu gat Asp cag Gin gtg Val 190 ggc Gly gcc Ala atc Ile cat His gtg Val1 ttg Leu aa t Asn act Thr atc Ile acc Thr gga Gly gat Asp ggc Gly 175 cgc Arg tat Tyr gat Asp gac Asp caa Gin cc t Pro cca Pro ttg Leu 80 aaa Lys gtg Val1 ttc Phe caa Gin gac Asp 160 gag Giu Gly ccc Pro ggc Gly aag Lys 240 atc Ile atg Met gag Giu gaa Giu gca Ala ctg Leu 115 aat Asn ggc Gly att Ile cgc Arg gtc Val 195 aa t Asn gca Ala tcc Ser acc Thr ggc Gly gtg Val cca Pro tgt Cys ggt Gly 100 cat His ggc Gly gtg Val tgc Cys gcg Ala 180 cgt Arg c tc Leu ggc Gly cgc Arg att Ile gag Glu ac c Thr acc Thr ggc Gly ctg Leu gcg Ala gc t Ala aat Asn tcc Ser 165 aac Asn ggt Gly tac Tyr tgg Trp gat Asp 245 163 211 259 307 355 403 451 499 547 595 643 691 739 787 835 883 atc gac ggc acc atg gtt cca ggc gtg cgt tac caa act gcc att tcc WO 01/00843 Ile Asp Gly Thr Met Val Pro Gly Val 250 gtg ggc acc aat ccc acc ttc ggc gat Val Gly Thr Asn Pro Thr Phe Gly Asp 265 270 ttc atc ctc gac cag gaa gcc gac ctg Phe Ilie Leu Asp Gin Glu Ala Asp Leu 280 285 gaa ttc gtg gga cac ttg cgc gac atg 1027 Giu Phe Vai Giy His Leu Arg Asp Met 295 300 gag cta cta gac gcc atg gcc cga gat 1075 Giu Leu Leu Asp Ala Met Ala Arg Asp 310 315 ctt gcc aaa gac aaa ttg ctt ctc gac 1123 Leu Ala Lys Asp Lys Leu Leu Leu Asp 330 taaggccggt caccggccat caa 1146 <210> 574 <211> 341 <212> PRT <213> Corynebacterium giutainicum <400> 574 Val Asp Ile Trp Ser Gly Leu Asp Ser 1 5 Ser Val Val Thr Ile Gly Val Phe Asp 25 Ser Leu Ilie Gly Giu Ala Lys Lys Gin Cys Val Met Val Thr Phe Asp Pro His 55 Gly Lys Giu Pro Thr Arg Leu Ala Pro 70 Ala Ala Giu Cys Gly Val Asp Ala Ala Giu Leu Ala Gly Leu Ser Ala Giu Glu 100 105 Asp Thr Leu His Ala Arg Ser Val Val 115 120 Gly Val Asn Gly Ala Gly Thr Giu Ser Tyr cga Arg ggt Gly aaa Lys acc Thr 320 gac Asp Pro Leu Glu Ile Asp 75 Val Phe Giy Met Gin cgc Arg cac His ttc Phe 305 aac Asn ac c Thr Al a His Glu Ala Tyr Ile Thr Giu Arg Ala gtc Val 275 gtc Val ggc Gly cgc Arg ccc Pro Leu Gly Gly Phe Leu Phe Met 110 Phe Leu PCT/EB00100923 Ile Ser 260 gag gca 931 Glu Ala atg gtg 979 Met Val gtc gac Val Asp gac atc Asp Ile 325 agc gct Ser Ala 340 Gin His Val1 Leu Asn Thr Ile Thr Gly Gly Gin Pro Pro Leu Lys Val Phe Gin WO 01/00843 WO 0100843PCTIiBOO/00923 Lys 145 Gin Val1 Giu Thr Val 225 Giu Gin Arg H-i s Phe 305 Asn Thr Gly Ile Arg Vai 195 Asn Ala Ser Aia Vali 275 Val1 Giy Arg Pro Val Asn Val Thr Ile Aia Pro Leu Leu His Asp Asp Asp Cys Aia 180 Arg Leu Gly Arg Ilie 260 Giu Met Val1 Asp Ser 340 Ser 165 Asn Gly Tyr Trp Asp 245 Ser Aia Vai Asp Ile 325 Ala 150 Thr Trp Ala Leu Phe 230 Ile Vai Phe Glu Glu 310 Leu Leu Tyr Glu Leu 220 Arg Pro Phe Ala Arg 300 Aia Leu Asp Ala Leu 205 Pro Giu Gly Gly Asp 285 Asp Arg Leu Giy 175 Arg Tyr Asp Asp Arg 255 Giu Tyr Val1 Vali Ala 335 <210> 575 <211> 805 <212> DNA <2i3> Corynebacteriun giutainicum <22 0> <221> CDS <222> (87)..(782) <223> RXN01712 <400> 575 gccggcttag gctcctccga ccagaacccc actggcacct ttggataaca ccattccgtg taaacaagct ggcctagact tgaaacatg gtc gat atc ttg gaa ctc atc ggt 113 Met Vai Asp Ile Leu Giu Leu Ile Giy 1 ccc cta cct ttt gtg tct acg cca gag tta agg gca att gtc gtg act 161 Pro Leu Pro Phe Val Ser Thr Pro Glu Leu Arg Ala Ile Val Val Thr WO 01/00843 gcc att, aa Ala Ilie As aat tcc ac Asn Ser Th gtg gtg tt Vai Vai Le 6 gtg gag gt Val Glu Va gaa gcg at Glu Ala Il gtg gat ac Vai Asp Tb acg gat aa Thr Asp As gct cag gt Ala Gin Va 14 aag ctt aq Lys Leu Ax 155 acg ttg ta Thr Leu T-y 170 cac acg at His Thr Ii aag ggc gg Lys Gly Gi gtc gat ga 15 acc Thr gaa Glu tcg Ser gaa Glu att Ile 95 ttc Phe caa Gin gtg Val1 ggt Giy atg Met 175 acg Thr gcg Aia acc act Thr ctt Leu agc Ser atc Ile gcg Ala ctt Leu gca Ala gag Giu tac Tyr 160 t tg Leu t tg Leu ccg Pro cta att Ile ctg Leu acg Thr cag Gin gtg Val gag Glu aag Lys acg Thr 145 gcc Ala gcc Ala tcg Ser cgc Arg ttc Phe aat Asn ttg Leu gtg Val aag Lys atg Met gcc Ala cag Gin 130 ttg Leu cgc Arg gcc Ala ggc Gly cga Arg 210 t tg Leu PCTIIBOO/00923 ctt gga 209 Leu Gly tcg gac 257 Ser Asp ggt ggc 305 Gly Gly ggt cag 353 Gly Gin ttt gat 401 Phe Asp 105 atc atc 449 Ile Ile 120 gct ggg 497 Ala Gly gtc gaa 545 Val Glu ggt gca 593 Gly Ala tgg cat 641 Trp His 185 acg gtg 689 Thr Val 200 gtc ttt 737 Val Phe aag 782 Lys Val Asp Asp Asp Ser Thr Leu 220 tgagtgttgg actctccgga tct <210> 576 <211> 232 <212> PRT <213> Corynebacterium giutanicwn WO 01/00843 WO 0100843PCTIiBOO/00923 <400> 576 Met Val Asp Ile Leu Glu Leu Ile Gly Pro Leu Pro Phe Val Ser Thr 10 Pro Glu Leu Arg Ala Ile Val Val Thr Ala Ile Asn Gly Ser Thr Thr 25 Ile Asn Gly Thr Ser Gly Gin Leu Gly Asn Ser Thr Asp Thr Giu Leu 40 Leu Leu Ala Leu Arg Arg Trp Ser Asp Val Val Leu Val Gly Ser Ser 55 Thr Val Lys Ala Giu Asn Tyr Gly Gly Val Giu Val Ser Pro Giu Ile 70 75 Gin Lys Gin Arg Gin Giu Leu Giy Gin Giu Ala Ile Pro Pro Ile Ala 90 Val Met Ser Gly Ser Leu Asn Phe Asp Val Asp Thr Arg Phe Phe Leu 100 105 110 Giu Ala Giu Val Pro Pro Ile Ile Ile Thr Asp Asn Ser Asp Gin Ala 115 120 125 Lys Gin Gin Arg Leu Val Asp Ala Gly Ala Gin Val Ile Giu Val Giu 130 135 140 Thr Leu Thr Ala Giu Val Gly Val Giu Lys Leu Arg Ser Leu Gly Tyr 145 150 155 160 Ala Arg Ile Asp Cys Giu Gly Gly Ala Thr Leu Tyr Gly Gin Met Leu 165 170 175 Ala Ala Asp Leu Vai Asp Val Trp His His Thr Ile Asp Pro Thr Leu 180 185 190 Ser Gly Ser Val Giu Arg Pro Thr Val Lys Gly Gly Asp Asp Ala Pro 195 200 205 Arg Arg Phe Ala Leu Giu His Val Phe Val Asp Asp Asp Ser Thr Leu 210 215 220 Phe Leu Arg Tyr Lys Arg Ala Lys 225 230 <210> 577 <211> 578 <212> DNA <213> Corynebacterium giutarnicum <22 0> <221> CDS <222> (55)..(555) <223> FRXA01712 <400> 577 cttctgttgg cgcttcgcag gtggtcggac gtggtgttgg ttgggtcgag cacggtg 57 Val WO 01/00843 WO 0100843PCTIIBOO/00923 1 aag gct gaa aat tat ggt ggc gtg gag gtt tcg cct gaa atc cag aag 105 Lys Ala Giu Asn Tyr Gly Gly Val Glu Val Ser Pro Giu Ile Gin Lys 10 caa cgc cag gag ttg ggt cag gaa gcg att ccg ccg att gcg gtg atg 153 Gin Arg Gin Giu Leu Gly Gin Giu Ala Ile Pro Pro Ile Ala Val Met 25 tca ggg tcg ttg aat ttt gat gtg gat act cgc ttt ttc ctt gag gcc- 201 Ser Gly Ser Leu Asn Phe Asp Val Asp Thr Arg Phe Phe Leu Giu Ala 40 gaa gtg ccg ccg atc atc atc acg gat aat tcc gat caa gca aag cag 249 Giu Val Pro Pro Ile Ile Ile Thr Asp Asn Ser Asp Gin Ala Lys Gin 55 60 cag cgg ctt gtg gat gct ggg gct cag gtt att gag gtg gag acg ttg 297 Gin Arg Leu Val Asp Ala Gly Ala Gin Val Ile Giu Val Giu Thr Leu 75 acg gcg gag gtt ggc gtc gaa aag ctt agg tct ttg ggt tac gcc cgc 345 Thr Ala Giu Val Gly Val Giu Lys Leu Arg Ser Leu Gly Tyr Ala Arg 90 att gat tgt gag ggc ggt gca acg ttg tat ggg cagr atg ttg gcc gcc 393 Ilie Asp Cys Giu Gly Gly Ala Thr Leu Tyr Gly Gin Met Leu Ala Ala 100 105 110.

gat ctt gtt gat gtg tgg cat cac acg att gat ccg acg ttg tcg ggc 441 Asp Leu Val Asp Val Trp His His Thr Ile Asp Pro Thr Leu Ser Gly 115 120 125 agc gtg gag cgc ccc acg gtg aag ggc ggc gat gat gcg ccg cgc cga 489 Ser Val Giu Arg Pro Thr Val Lys Gly Gly Asp Asp Ala Pro Arg Arg 130 135 140 145 ttc gcg ttg gag cac gtc ttt gtc gat gat gac agc acc cta ttc ttg 537 Phe Ala Leu Giu His Val Phe Val Asp Asp Asp Ser Thr Leu Phe Leu 150 155 160 cgg tat aag cgc gcc aag tgagtgttgg actctccgga tct 578 Arg Tyr Lys Arg Ala Lys 165 <210> 578 <211> 167 <212> PRT <213> Corynebacteriun giutanicun <400> 578 Val Lys Ala Giu Asn Tyr Gly Gly Val Giu Val Ser Pro Glu Ilie Gin 1 5 10 Lys Gin Arg Gin Glu Leu Giy Gin Giu Ala Ile Pro Pro Ile Ala Val 25 Met Ser Gly Ser Leu Asn Phe Asp Val Asp Thr Arg Phe Phe Leu Giu 40 WO 01/00843 WO 0100843PCTJIBOO/00923 Ala Gin Leu Arg Ala Giy Arg 145 Leu Pro Leu Giu Cys 100 Val Giu Leu Lys Pro Val1 Val Giu Asp Arg Giu Arg 165 Asn Val1 Arg Tyr Ile Gly Asp 155 S er Ile Ser Gly Asp Asp 140 Asp <210> 579 <211> 831 <212> DNA <213> Corynebacteriun glutainicum <220> <221> CDS <222> (101)..(808) <223> RXN02384 <400> 579 ctgatgaggc ggatatccgc aacatcgaca gcattgatga aatacgagcc tggctacgag gacgattaag aggtagtcct att Ilie cag Gin gcc Ala agc Ser c tg Leu cga Arg ttg Leu gcc Ala ctt Leu gcg Ala actcccacct ttgccagctg gtg act cgt cgc ctg Val Thr Arg Arg Leu 1 gcc acg tcc cga atg Ala Thr Ser Arg Met ggc ttt caa cag gcg Gly Phe Gin Gin Ala atc acc cat gtg ttc Ile Thr His Val Phe agc gcg gtt gcg gcg Ser Ala Val Ala Ala ctt cgg gaa acg cat Leu Arg Giu Thr His tca gtg ctg Ser Val Leu tcc Ser gag Glu WO 01/00843 75 ttg ggt gag tgg cag gcc aaa acc cac Leu Gly Glu Trp Gin Ala Lys Thr His cca ggt gcg cgc gct caa tgg cgc cac Pro Gly Ala Arg Ala Gin Trp Arg His 105 110 ggc ggc gaa tcg cgc gtg gat gtt gcg Gly Gly Giu Ser Arg Val Asp Val Ala 120 125 gac gag ttg atg gtg tcg ctt gat gat Asp Glu Leu Met Val Ser Leu Asp Asp 135 140 atc gtg gct cac ggt ggc acg att aat Ile Val Ala His Gly Gly Thr Ile Asn 150 155 gac ctg gcg tat gat cag tac ccc atg Asp Leu Ala Tyr Asp Gin Tyr Pro Met 170 tgt tgg gca caa ttg acc gcc cga cct Cys Trp, Ala Gin Leu Thr Ala Arg Pro 185 190 aac cca gaa gat gac ctc aagatt tct' Asn Pro Giu Asp Asp Leu Lys Ilie Ser 200 205 cat ttt gag ggc aac aat gtg gaa aac His Phe Giu Giy Asn Asn Val Glu Asn 215 220 tgg aac atg ggt gtt acg cag taaagaa Trp Asn met Gly Val Thr Gin 230 235 <210> 580 <211> 236 <212> PRT <213> Corynebacteriun giutainicum <400> 580 Vai Thr Arg Arg Leu Ile Leu Leu Arg 1 5 Ala Thr Ser Arg Met Gin Gly Gin Leu .25 Gly Phe Gin Gin Ala Ala Ser Ala Ala 40 Ile Thr His Val Phe Ser Ser Asp Leu 55 gtg Val cag Gin gca Ala gaa Glu 145 ac c Thr gga Gly tat Tyr gtt Val1 tgg Trp 225 gat tcc gaa Asp Ser Glu 100 tgg gca cca Trp Ala Pro 115 cgc caa gtt Arg Gin Vai 130 ggc acc gtg Gly Thr Val tcg aat ctt Ser Asn Leu ctt gga aat Leu Gly Asn 180 gca ggt agt Ala Gly Ser 195 tcc aac agc Ser Asn Ser *210 tat ctt gac Tyr Leu Asp TIiBOO/00923 tat 403 Tyr ccc 451 Pro gtc 499 Val ctc 547 Leu ttg 595 Leu 165 acc 643 Thr gag 691 Glu cct 739 Pro ggc 787 Gly 831 Ala Gin ;at ggcaataaaa atg His Gly Gln Thr Giu Tyr Asn 10 Asp Thr Giu Leu Ser Asp Leu Ser Vai Leu Val Gin Lys Asn Ser Arg Aia Phe Asn Thr Ala WO 01/00843 Ser Ala Va Leu Arg Gi Val Asp Se Gin Trp Al 11 Ala Arg GI 130 Giu Gly Tlh 145 Thr Ser As Gly Leu GI Tyr Ala GI 19 Val Ser As 210 Trp Tyr Ire 225 <210> 581 <211> 453 <212> DNA Ile Gly Gly Gly Giu 135 Val Leu Trp Pro Phe 215 Asn Asp His His 110 Ala Asp Asn Met Pro 190 Ser Asn PCTIBOO/00923 Lys Arg Thr Giu Asp Pro Arg Arg Trp Asp Ala Leu 160 Phe Ser 175 Arg Tyr Ser Ala Ala Gin <213> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(430) <223> RXN01560 <400> 581 atgggagcaa ggctcattta gctacttcga cgtggaagcg cacatcgttg agttgattcc tgcatcagta agcgatgcgt tttaggcaca tctgcgatcc gtg ggc gtg tcc tac Val Gly Val Ser Tyr 1 atc atc gcc ggc gat gag cag ctg gat atg gca gaa gcc gtt cgc aaa Ilie Ile Ala Gly Asp Glu Gin Leu Asp Met Ala Giu Ala Val Arg Lys 15 att ggg gag acc ttt aaa act gag gaa att atc ctt ggt ggc gga gga Ilie Gly Glu Thr Phe Lys Thr Glu Glu Ilie Ilie Leu Giy Gly Gly Gly 30 acc ctg aac tgg tcc atg ctc cgc gac ggt ttg tgc gac gag gtt agc.

Thr Leu Asn Trn Ser Met~ Len Ama Aern Gl Leu Cvs Asn Giu Val Ser 115 163 211 259 827 WO 01/00843 WO 0100843PCT/EBOO/00923 45 atc gtg atg atg cca atc gcc gat Ilie Val Met Met Pro Ile Ala Asp 60 ttc gaa gcc gat gaa aaa tac tca Phe Glu Ala Asp Glu Lys Tyr Ser 75 ctc gcc agc gtt gaa cca cta gaa Leu Ala Ser Val Glu Pro Leu Giu ggg gtc aat ggc cca gtg gac gcg Gly Val Asn Gly Pro Val Asp Ala 105 ttt <210> 582 <211> 110 <212> PRT <213> Corynebacterium glutamicun <400> 582 Val Gly Val Ser Tyr Ile Ile Ala 1 .5 Glu Ala Val Arg Lys Ile Gly Giu Leu Gly Gly Gly Gly Thr Leu Asn 40 Cys Asp Glu Val Ser Ile Val Met 55 His Thr His Ser Leu Phe Glu Ala 70 Pro Ile Gly Phe Ser Leu Ala Ser Val Trp, Met Arg Tyr Gly Val Asn 100 ggt gaa aag cac acc cac tct Gly Glu Lys His Thr His Ser gca ccg ttg ccg atc ggt ttt Ala Pro Leu Pro Ile Gly Phe 80 gat gga agc gtt tgg atg cgt Asp Gly Ser Val Trp Met Arg 95 100 aac taggtagcaa atactcgctc Asn 110 t tg Leu t ca Ser tac Tyr Gly Thr 25 Trp Met Asp Val Gly 105 Asp Phe Ser Pro Giu Glu Pro Giu Lys Met Ile

LYS

Pro Val Gin Thr Leu Ala Tyr Leu Asp Leu Glu Arg Asp Ser Giu Ala Asp Glu Asp Gly Ala Asp Asn 110 <210> 583 <211> 1137 <212> DNA <213> Corynebacteriun giutaxnicum <220> <221> CDS <222> (101)..(1114) <223> RXN00667 <400> 583 ggcatcagtg tttgaaggga aaagcaggtc aaacaaggtg cggctgattt gagcgatcac WO 01/00843 WO 0100843PCTAIBOO/00923 agcaccgaga tctgtgctga agaattcaat gattgggttg atg att gcg ttg Met Ile Ala Leu 1 tcc atg tct aac Ser Met Ser Asn gta gta caa aag Val Val Gin Lys aaa atg aaa gcg Lys Met LYS Ala ccg cta Pro Leu ccc atc cgc Pro Ilie Arg gcg gcg ccg Ala Ala Pro gac Asp ggc ctc: aac cct Gly Leu Asn Pro cgt gtg cgc ttg Arg Vai Arg Leu ccg ctc gac Pro Leu Asp att tcc acg Ile Ser Thr atc cgc gcc atc Ile Arg Ala Ilie ttt gtt gaa tac Phe Val Giu Tyr cag cgc Gin Arg cac cgc aat ccg His Arg Asn Pro gcc Ala 60 gac aac gcc gaa Asp Asn Ala Giu gcg Ala ctt caa gcg cgt Leu Gin Ala Arg gac gcc gac ctt Asp Ala Asp Leu gtc aac cac tac Val Asn His Tyr gag ccc tac gcc Giu Pro Tyr Ala gac acc atg gtt Asp Thr Met Val ccc gac gac gac Pro Asp Asp Asp tgg ttc tac cgc Trp Phe Tyr Arg atg ccc Met Pro 100 gcc gcc gaa Ala Ala Giu gat gac atc Asp Asp Ile 120 cgg Arg 105 ccg atc cct tac Pro Ile Pro Tyr att cat gtc att cac gaa gac Ile His Val Ile His Giu Asp 115 ctc gtc att gac Leu Val Ile Asp aag Lys 125 cca ccc tac cta Pro Pro Tyr Leu acc atg cct Thr Met Pro cgt ggc Arg Gly 135 cgc cac atc acc Arg His Ile Thr gaa Giu 140 acc gct ctg gtg Thr Ala Leu Val aaa Lys 145 atg cgt gtg ctg Met Arg Val Leu act Thr 150 gga aac aac gat Gly Asn Asn Asp ctc Leu 155 acc cca gct cac Thr Pro Ala His ctc gat cgc ctg Leu Asp Arg Leu tcc ggt gtg tta Ser Gly Val Leu atg gtg aaa aaa Met Val Lys Lys gaa ctc cgt ggc Giu Leu Arg Gly gct tac Ala Tyr 180 caa acc ttg Gin Thr Leu gca gaa ttc Ala Giu Phe 200 gcc cga cgt gag Ala Arg Arg Giu tcc aaa acc tat Ser Lys Thr Tyr gag gca atc Giu Ala Ile 195 att tgg gaa Ile Trp Giu gtt cca ggg cta Val Pro Giy Leu gat gat ggt ccc Asp Asp Gly Pro tcc cgc Ser Arg 215 atc gaa aaa gaa Ilie Giu Lys Giu cgc Arg 220 ggc atc gtg caa Gly Ile Val Gin gcc Ala 225 ttc gtc gtg gaa Phe Val Val Glu WO 01/00843 ggc ccc gtc aac gca cgc act gaa c Gly Pro Val Asn Ala Arg Thr Giu I 230 235 gac gcc gaa cag agc atc ctc gaa c Asp Ala Giu Gin Ser Ile Leu Giu 250 caa gcg cgc tac gtt cta gcc cccI Gin Ala Arg Tyr Val Leu Ala Pro 265 cgc atc cac atg cgc gac ttc gca c Arg Ile His Met Arg Asp Phe Ala 2 280 285 tac ccc gtc ctc cac gca gtc gac c 1027 Tyr Pro Val Leu His Ala Val Asp 295 300 cac ctc atc gcc cgc acg cta acc1 1075 His Leu Ilie Ala Arg Thr Leu Thr 1 310 315 gaa cgt acc ttt gtg agt aat cga 1124 Giu Arg Thr Phe Val Ser Asn Arg 1 330 agacatcacc cag 1137 <210> 584 <211> 338 <212> PRT <213> Corynebacterium giutanicum <400> 584 met Ile Ala Leu Lys Ser Met Ser I 1 5 Met Lys Ala Pro Leu Pro Ile Arg 2 Arg Leu Pro Leu Asp Ala Ala Pro 40 Tyr Leu Ile Ser Thr Gin Arg His 1 Ala Leu Gin Ala Arg Phe Asp Ala 1 Giu Pro Tyr Ala Pro Asp Thr Met 1 Phe Tyr Arg Met Pro Ala Ala Giu 100 gtg Val atg Met 255 aca Thr ccc Pro gag Giu gtg Val acg Thr 335 Arg 10 Gly Arg Asn Leu Gin 90 Pro acc Thr cca Pro acc Thr ggC Gly 290 acc Thr caa Gin t tg Leu Gin Pro Asp Asp Asn Asp Tyr PCTABOO/00923 cca gtc gaa 835 Pro Val Giu 245 ctc ccc cgc 883 Leu Pro Arg 260 cac cag ctg 931 His Gin Leu 275 gac ccc ctc 979 Asp Pro Leu acc cca atg Thr Pro Met acc aac gag Thr Asn Giu 325 taggcctcgt Lys Ser Phe Asn His Asp Lys 110 Pro Arg Val Ala Tyr Ile Ile WO 01/00843 WO 0100843PCTIBOOIOO923 Val1 Leu Lys 145 Leu Leu Thr Pro Ala 225 Val Gly Lys Leu Tyr 305 Pro Ser Ile Ala 130 Met Asp Arg Tyr Ala 210 Phe Thr Pro Thr Gly 290 Thr Gin Leu His 115 Thr Arg Arg Gly Giu 195 Ile Val Pro Leu His 275 Asp Thr Thr Giu Asp Asp Asp Ile Leu Val Ile Asp Lys Pro Pro Tyr Met Val Leu Ala 180 Ala Trp Val Val Pro 260 Gin Pro Pro Asn Pro Leu Thr 165 Tyr Ilie Glu Giu Glu 245 Arg Leu Leu Met Giu 325 Arg Thr 150 Ser Gin Ala Ser Gly 230 Asp Gin Arg Tyr His 310 Giu Gly 135 Gly Giy Thr Giu Arg 215 Pro Aia Ala Ile Pro 295 Leu Arg 120 Arg Asn Val Leu Phe 200 Ile Val Giu Arg His 280 Val Ile Thr 125 His Asn Leu Phe 185 Val1 Giu Asn Gin Tyr 265 Met Leu Ala Phe Ile Asp Val 170 Ala Pro Lys Ala Ser 250 Val Arg His Arg Val 330 Thr Leu 155 Met Arg Gly Giu Arg 235 Ile Leu Asp Ala Thr 315 Ser Glu 140 Thr Val Arg Leu Arg 220 Thr Leu Ala Phe Val 300 Leu Asn Thr Pro Lys Giu Leu 205 Gly Giu Glu Pro Ala 285 Asp Thr Arg Ala Leu Ala His Lys Pro 175 Ala Ser 190 Asp Asp Ile Val Leu Val Glu Met 255 Ser. Thr 270 Ala Pro Asp Glu Phe Val Pro Thr 335 Val1 Arg 160 Glu Lys Gly Gin Ser 240 His Gly Ile Asp Asp 320 Gly.

<210> 585 <211> 1281 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1258) <223> RXCO1711 <400> 585 tctcgtgagt ttctccccgg tagcaccttc tatatcagcc cccacgccgc gtcggagcag gtgggatagc atcggcaacg cggttgcatg gccgttggcc atg ttg ttg atg gcg 115 831 WO 01/00843 WO 0100843PCT/IBOO/00923 Met Leu Leu Met 1 cat cgc ttc ttc His Arg Phe Phe ctt gcg att aac Leu Ala Ile Asn ggc Gly gca gtc acc gac Ala Val Thr Asp gat ttc Asp Phe acg acg gtt Thr Thr Val tac aac gag Tyr Asn Giu tat Tyr agt gct tta cga Ser Ala Leu Arg ttc gtt gaa ggt Phe Val Giu Gly att ccg gtc Ile Pro Val tat aac ccg Tyr Asn Pro gtc tac cac ttc gtc gat ccg cac tac Val Tyr His Phe Val Asp Pro His Tyr ggc gcc Gly Ala acc ctc cta ttg Thr Leu Leu Leu cca ttg gga tat Pro Leu Gly Tyr acc cat ttc acg Thr His Phe Thr gct cgg tgg atg Ala Arg Trp Met atc gcg gtg Ile Ala Val aac ctc ctt gcc att gtt Asn Leu Leu Ala Ile Val gcg ttc ggg ctg Ala Phe Gly Leu acc aga ctc tcc Thr Arg Leu Ser ggt Gly tgg gcg ctg cgc Trp Ala Leu Arg agc atg Ser Met 100 gtg tgg ccg Val Trp Pro aac acc ctc Asn Thr Leu 120 gcg atc gcc ttg Ala Ile Ala Leu atg ctg aca gaa Met Leu Thr Glu acc gtg caa Thr Val Gin 115 att ttc tcc aac Ile Phe Ser Asn aac ggc atc ctg ctg ctc atg ttg Asn Gly Ile Leu Leu Leu Met Leu 130 gcg att Ala Ile 135 ttc ctg tgg tgc Phe Leu Trp Cys gtg cac aaa aaa Val His Lys Lys tcc Ser 145 tgg ttg ggc gga Trp Leu Gly Gly gtc att ggt ttg Val Ilie Giy Leu att ttg atc aaa Ile Leu Ile Lys atg ttc ctg cca Met Phe Leu Pro ctt Leu 165 499 54.7 595 643 691 739 ctc ttc cta cct Leu Phe Leu Pro gtg aaa aag caa Vai Lys Lys Gin tgg T rp 175 gga tcg ctc atc Gly Ser Leu Ile ct-c ggc Leu Gly 180 att tta acc Ile Leu Thr gga gca tct Gly Ala Ser 200 gtg att ttc aat Val Ilie Phe Asfl gca Ala 190 gtg gcc tgg ttc Val Ala Trp Phe tta gtt ccg Leu Val Pro 195 ggt gaa act Gly Giu Thr gaa tac gtc acc Glu Tyr Val Thr acg atg ccc tac Thr Met Pro Tyr cga gat Arg Asp 215 ttt gcc aac agc Phe Ala Asn Ser tca Ser 220 ctc cca ggc ttg Leu Pro Gly Leu gcc Aia 225 atc tat ttc gga Ile Tyr Phe Gly atg ccc acc tgg atg gaa atc acc tgg ttc ctc atc ttc ggc gca atg 'Met Pro Thr Trp, met Glu Ile Thr Trp Phe Leu Ile Phe Gly Ala Met 835 WO 01/00843 PCTIBOOIOO923 230 235 240 245 gtc ggc ctc gca gtg ctg gca ctc ctg aga ttc cgt aac acc gag cca 883 Val Gly Leu Ala Val Leu Ala Leu Leu Arg Phe Arg Asn Thr Glu Pro 250 255 260 tac ttc tgg gca gca acc acc acc ggt gta ctc ctg act ggc gta ttc 931 Tyr Phe Trp Ala Ala Thr Thr Thr Gly Val Leu*Leu Thr Gly Val Phe 265 270 275 ttc ctg tcc tca ctg gga cag atg tac tac tcc atg atg atc ttc cct 979 Phe Leu Ser Ser Leu Gly Gin Met Tyr Tyr Ser Met Met Ile Phe Pro 280 285 290 atg atc ttc acc ctg ctc gga agc cga tcc gta ttc cac aac tgg gtt 1027 Met Ile Phe Thr Leu Leu Gly Ser Arg Ser Val Phe His Asn Trp Val 295 300 305 gcc tgg gtc gcc gcc tac ttc tta cta tcc cct gac act ttc acc tcc 1075 Ala Trp Val Ala Ala Tyr Phe Leu Leu Ser Pro Asp Thr Phe Thr Ser 310' 315 320 325 cag cga cta ccc gat gta gcc cgc tgg atg gaa. ttt ttc agc gcg acc 1123 Gin Arg Leu Pro Asp Val Ala Arg Trp Met Glu Phe Phe Ser Ala Thr 330 335 340 gtt ggt tgg gga cta ttg ata gtg gtt aca ttt gtc tcg gcg cta atc 1171 Val Gly Trp Gly Leu Leu Ile Val Val Thr Phe Val Ser Ala Leu Ilie 345 350 355 tgg ttt att ggt gat atc cga gcc aag gga act ccg agc tca ccc att 1219 Trp Phe Ile Gly Asp Ile Arg Ala Lys Gly Thr Pro Ser Ser Pro Ile 360 365 370 acc act gat cca acg cac gac cat ctt gag agg aca gca tgacagactt 1268 Thr Thr Asp Pro Thr His Asp His Leu Glu Arg Thr Ala 375 380 385 caaactcatc agc 1281 <210> 586 <211> 386 <212> PRT <213> Corynebacterium giutamicun <400> 586 Met Leu Leu Met Ala His Arg Phe Phe Val Leu Ala Ile Asn Gly Ala 1 5 10 Val Thr Asp Asp Phe Thr Thr Val Tyr Ser Ala Leu Arg Arg Phe Val 25 Giu Gly Ilie Pro Val Tyr Asn Giu Val Tyr His Phe Val Asp Pro His 833 WO 01/00843 WO 0100843PCT/IBOO/00923 Tyr Ile Leu Ala Thr Leu Ser 145 Met Ser Trp Tyr Ala 225 Ile Arg Leu Met Phe 305 Asp Phe Leu Thr Ala Leu Glu Leu 130 Trp Phe Leu Phe Leu 210 Ile Phe Asn Thr Met 290 His Thr Phe Tyr His Ilie Arg Thr 115 Leu Leu Leu Ilie Leu 195 Gly Tyr Gly Thr Gly 275 Ilie Asn Phe Ser Ala 55 Ala Phe Trp, Thr Ile 135 Val1 Phe Leu Al a Asp 215 Pro Gly Phe Leu Ile 295 Trp Arg Gly Thr Arg Gly Pro Leu 120 Phe Ile Leu Thr Ser 200 Phe Thr Leu Trp Se r 280 Phe Val Leu Trp Leu Met Leu Ala Phe Trp Leu Leu 170 Val Tyr Asn Met Val 250 Ala Leu Leu Ala Asp 330 Leu Asp Ala Ile Arg Ala Asn Val 140 Ile Lys Phe Thr Ser 220 Ile Ala Thr Gin Gly 300 Phe Ala Ile Arg Leu Val Ser Ala 110 Asn His Ile Gin Ala 190 Thr Pro Trp Leu Gly 270 Tyr Arg Leu Trp Val 350 Lys Gly Asn Gly Met Gly Lys Lys Trp 175 Val1 Met Gly Phe Arg 255 Val Tyr Ser Ser Met 335 Thr Gly Tyr Leu Trp Leu Ile Lys Pro 160 Gly Ala Pro Leu Leu 240 Phe Leu Ser Val1 Pro 320 Giu Phe Thr Val Ser Ala 355 Leu Ilie Trp Phe Ile Gly 360 WO 01/00843 WO 0100843PCTIiBOO/00923 Pro Ser Ser Pro Ile Thr Thr Asp Pro Thr His Asp His Leu Giu Arg 370 375 380 Thr Ala 385 <210> 587 <211> 777 <212> DNA <213> Corynebacteriun <22 0> <221> CDS <222> (101)..(754) <223> RXC02380 <400> 587 gcagattttt gacgaagtcg giutamicun catcgtggat tgaagctgcc taaaaactcg cgaggacgca tgctcagcaa ggtggtgtgc gaacactata ctgtccactc atg act aca acg Met Thr Thr Thr aaa cgc cgc gct cgc att. ggc atc atg Lys Arg Arg Ala Arg Ilie Gly Ile Met ggc aca ttt gac Gly Thr Phe Asp ccc att Pro Ile cat aat ggt His Asn Gly ctt gat ctg Leu Asp Leu ctt gtg gcg ggc Leu Val Ala Gly gag gta gcg gat cga ttc gat Giu Val Ala Asp Arg Phe Asp gtg gtg tac gtt Val Val Tyr Val acc gga cag cca Thr Gly Gin Pro caa aag gcg Gin Lys Ala aac aag Asn Lys aaa gtc agc cca gcg gaa gat cgt tac Lys Val Ser Pro Ala Giu Asp Arg Tyr atg acg gtg atc Met Thr Val Ile act gcc tct aat Thr Ala Ser Asn cgg ttt atg gta Arg Phe Met Val tcg Ser cgg gtt gat att Arg Val Asp Ile cgg gga ggg gat Arg Gly Gly Asp tac acg atc gat Tyr Thr Ile Asp ctg caa gat ttg Leu Gin Asp Leu agc aag Ser Lys 100 caa tac ccg Gin Tyr Pro gca cag atc Ala Gin Ile 120 gac Asp 105 gcc cag ctg tac Ala Gin Leu Tyr atc acc ggt gcc Ile Thr Gly Ala gat gca ctg Asp Ala Leu 115 gaa ctt gcc Giu Leu Ala gtg acg tgg cgc Val Thr Trp Arg tgg gag aaa acc Trp Glu Lys Thr ttc Phe 130 cac ttc His Phe 135 gtt gga gtg act Val Gly Val Thr ccc ggt tat gaa Pro Gly Tyr Giu ttg Leu 145 gat gga aac atc Asp Gly Asn Ile WO 01/00843 att ccg gaa atg cac caa gat cga Ilie Pro Glu Met His Gin Asp Arg 150 155 atg gct att tcc tcc acg gac tgc Met Ala Ile Ser Ser Thr Asp Cys 170 cct gtt tgg tat ctt gtc cct gat Pro Val Trp Tyr Leu Val Pro Asp 185 cgc caa ctc tat cga cct gaa gga Arg Gin Leu Tyr Arg Pro Giu Gly 200 205 ggc caa aac caa gca taaatctagt c~ Gly Gin Asn Gin Ala 215 <210> 588 <211> 218 <212> PRT <213> Corynebacterium giutanicun gtc tca ttg g lTai Ser Leu V 160 aga gaa cgc t krg Glu Arg S 175 9gC gtg gtg c Giy Val Val G 190 tcc gat aag g Ser Asp Lys A aaaagtttc aac tg al cc er aa in at sp gat Asp agc Ser tac Tyr atg Met 210 PCTIBOOOO923 atc ccc gcc 595 Ile Pro Ala 165 gaa gaa cgc 643 Giu Glu Arg 180 att gcc aaa 691 Ile Ala Lys 195 gat ccc aag 739 Asp Pro Lys <400> 588 Met Thr Thr Thr Val Lys Arg Arg Ala Thr Phe Ala Asp Pro Trp Leu Met Arg Val Gin Asp Gly Ala Thr Phe 130 Leu Asp 145 Vai Asp Pro Phe Lys Val1 Ilie Ser 100 Al a Leu Asn Pro Arg Leu Val Ser Asn Thr Ala Thr Val His Ser 170 Gly Ala Val Ala Arg Thr Leu Arg Arg 140 Asp Asp Ile Gly Pro Giu Phe Ile Tyr Asp 125 Pro Arg Cys Met Ser Thr Asp Met Asp Phe 110 Trp, Gly Val Arg Ser Ser Giu Glu Arg Pro Val Trp Tyr Leu Val Pro Asp Gly Val Val WO 01/00843 WO 0100843PCT/EBOO/00923 180 185 190 Gin Tyr Ile Ala Lys Arg Gin Leu Tyr Arg Pro Giu Gly Ser Asp Lys 195 200 205 Asp Met Asp Pro Lys Gly Gin Asn Gin Ala.

210 215 <210> 589 <211> 777 <212> DNA <213> Corynebacterium giutanicun <220> <221> CDS <222> (101)..(754) <223> FRXA02380 <400> 589 gcagattttt gacgaagtcg catcgtggat tgaagctgcc taaaaactcg cgaggacgca tgctcagcaa ggtggtgtgc gaacactata ctgtccactc atg act aca acg gtg Met Thr Thr Thr Val aaa Lys cat His ctt Leu aac Asn gcc Ala cgg Arg caa Gin gca Ala cgc Arg aat Asn gat Asp aag Lys act Thr gga Gly tac Tyr cag Gin cgc Arg ggt Gly ctg Leu aaa Lys gcc Ala ggg Gly ccg Pro atc Ile 120 gct Ala cac His gtg Val gtc Val1 tc t Ser gat Asp gac Asp 105 gtg Val cgc Arg ctt Leu gtg Val1 agc Ser aat Asn act Thr gcc Ala acg Thr ggc atc Gly Ile gcg ggc Ala Gly gtt ccc Val Pro 45 gcg gaa Ala Giu 60 cgg ttt Arg Phe acg atc Thr Ile ctg tac Leu Tyr cgc gat Arg Asp 125 ggt Gly 15 gag Giu gga Gly cgt Arg gta Val acc Thr 95 atc Ile 1 ggc aca Gly Thr gta gcg Val Ala cag cca Gin Pro tac ctg Tyr Leu 65 tcg cgg Ser Arg 80 ctg caa Leu Gin acc ggt Thr Gly aaa acc Lys Thr ttt Phe gat Asp tgg Trp 50 atg Met gtt Val1 gat Asp gcc Ala ttc Phe 130 ccc Pro 20 t tc Phe aag Lys gtg Val att Ile agc Ser 100 gca Ala ctt Leu att Ile gat Asp gcg Ala atc Ile gat Asp aag Lys ctg Leu gcc Ala 115 163 211 259 307 355 403 451 499 547 gat Asp ttc Phe 110 tgg gag Trp Glu ggt tat cac ttc gtt gga gtg act cga ccc gaa ttg gat gga aac atc His Phe Val Gly Vai Thr 135 Pro Gly Tyr Giu Leu Asp Gly Asn Ile 145 WO 01/00843 WO 0100843PCT/EBOO/00923 att Ilie 150 atg Met cc t Pro cgc Arg cac His tc c Ser 170 ctt Leu cga Arg caa gat cga gtc tca ttg gtg Gin Asp Arg Val Ser Leu Val 155 160 acg gac tgc aga gaa cgc tcc Thr Asp Cys Arg Giu Arg Ser 175 gtc cct gat ggc gtg gtg caa Val Pro Asp Gly Vai Val Gin 190 cct gaa gga tcc gat aag gat Pro Giu Giy Ser Asp Lys Asp 205 taaatctagt caaaagtttc aac ggc caa aac caa gca Gly Gin Asn Gin Ala 215 <210> 590 <211> 218 <212> PRT <213> Corynebacteriun giutanicun <400> 590 Met Thr Thr Thr Val Lys Arg Arg Ala 1 Thr Ala Pro Leu Arg Gin Gly Thr Leu 145 Val1 Phe Asp Trp Met Val1 Asp Ala Phe 130 Asp Asp Asp Arg Gin Thr Asp Leu Asp 115 Giu Gly Ile Pro Phe Lys Val1 Ilie Ser 100 Ala Leu Asn Pro 5 Ilie Asp Ala Ile Asp Lys Leu Ala Ile Ala 165 Asn Asp Lys 55 Thr Gly Tyr Gin Phe 135 Pro Ala Arg Leu Val1 Ser Asn Thr Ala Thr Val His Ser 170 Gly Ala Val1 Ala Arg Thr Leu Arg Arg 140 Asp Asp gat Asp agc Ser tac Tyr atg Met 210 Ile Gly Pro Giu Phe Ile Tyr Asp 125 Pro Arg Cys atc ccc gcc Ile Pro Ala 165 gaa gaa cgc Giu Glu Arg 180 att gcc aaa Ile Ala Lys 195 gat ccc aag Asp Pro Lys 595 643 691 739 777 Met Ser Thr Asp Met Asp Phe 110 Trp Gly Val1 Arg WO 01/00843 WO 0100843PCTIiBOO/00923 Ser Ser Glu Giu Arg Pro Val Trp Tyr 180 185 Gin Tyr Ile Ala Lys Arg Gin Leu Tyr 195 200 Leu Val Pro Asp Gly Val Val 190 Ser Asp Lys Arg Pro Glu Gly 205 Asp Met Asp Pro Lys Giy Gin Asn Gin Ala 210 215 <210> 591 <211> 594 <212> DNA <213> Corynebacteriun <22 0> <221> CDS <222> (101)..(571) <223> RXC02921 <400> 591 glutamicun tggaaaactg ggaagggttg acgttgcgga atctctccgc agcgtcggtt cggaccctaa aaaagggtga ggaaccacat gagctgtttt aaggaatttt gtg tct gca ctt gaa Val Ser Ala Leu Glu gag tcg atc cgc atc gcg acc atc gcg Glu Ser Ile Arg Ile Ala Thr Ilie Ala aaa gca gcg gat Lys Ala Ala Asp gaa aag GiU LYS aag gcc gat Lys Ala Asp acc gat tgc Thr Asp Cys atc gct gtc atc Ile Ala Val Ile gtc ict gac atg atc gca atc Val Ser Asp met Ile Ala Ile ttt gtt gtt gca Phe Val Val Ala gct gac aat gag Ala Asp Asn Glu cag gtg ggc Gin Val Gly gcc att Ala Ile gtt gag gag atc Val Giu Giu Ile gat gag atg acc aag gct ggt ttc gag Asp Giu Met Thr Lys Ala Gly Phe Glu aag cgc cgt gaa Lys Arg Arg Giu aac cgc gaa aac Asn Arg Giu Asn tgg gtt ctC Ctt Trp Val Leu Leu tac gga ttg gtt Tyr Gly Leu Val atc cac gtt cag Ile His Val Gin cag gca gag cgc Gin Ala Giu Arg gag ttc Giu Phe 100 tac gga ctg Tyr Gly Leu gga ctt gaa Gly Leu Giu 120 gat cgt ctg tac cgc gac tgc cca ctc att gaa att gaa Asp Arg Leu Tyr Arg Asp Cys Pro Leu Ile Glu Ile Glu 105 110 115 acc ttc aag cgt Thr Phe Lys Arg tcc tcc tgg tct Ser Ser Trp Ser gag gcg gat Giu Ala Asp atc cgc aac atc gac agc att gat gaa ctc cca cct ttg cca gct gaa lie Arg Asn Ilie Asp Ser Ilie Asp Giu Leu Pro Pro Leu Pro Ala Glu WO 01/00843 135 140 145 tac gag cct ggc tac gag gac gat taagaggtag tcctgtgact cgt Tyr Glu Pro Gly Tyr Glu Asp Asp 150 155 <210> 592 <211> 157 <212> PRT <213> Corynebacterium giutanicuxn PCTIiBOOIOO923 <400> 592 Val Ser Ala Leu Giu Giu Ser Ile Arg 1 Ala Asp Giu Lys Trp Ala Leu Ser Pro 145 Ala Met Arg Ala Val Giu Ile Asp 130 Leu Asp Ile Gin Gly Leu Arg Giu 115 Giu Pro 5 Lys Ile Gly Giu Asp Phe Glu Asp Giu Lys Thr Ala Pro 70 Tyr Tyr Gly Ile Tyr 150 Ala Asp Ile 55 Lys Gly G ly Leu Arg 135 Glu Asp Cys Val Arg Leu Leu Giu 120 Asn Pro Asp Phe Giu Arg Vai Asp 105 Thr Ile Gly Ile 10 Ile Val1 Giu Glu Val 90 Arg Phe Asp Tyr Thr Val Ala Giu Asn His Tyr Arg Ile 140 Asp Ala Asp Ala Giu Giu Gin Asp 110 Ser Glu Ala Val Asp Met Asn Arg Cys Ser Leu Lys Ser Asn Thr Arg Gin Pro Trp Pro <210> 593 <211> 3075 <212> DNA <213> Corynebacteriun giutanicun <22 0> <221> CDS <222> (101)..(3052) <223> RXC01434 <400> 593 ggtttcctgc gcaccgtgat gattggtgcg gcgctgtcgc cggccatcgc ttcggcgttc aacactgcca acacgctgcc caacctgatc actggaaatc gtg ttg ggt gcg gtg 115 Val Leu Gly Ala Val 1 WO 01/00843 ctg aca tcg ctg gtt att ccg gtc ctt acc cgc gcg gaa aaa Leu Thr Ser Leu Val Ile Pro Val Leu Thr Arg Ala Glu Lys PCT/EBOOIOO923 gcc Ala acg Thr ctg Leu t cc Ser ctg Leu ccc Pro ctg Leu gtg Val ctt Leu 150 gcg Ala caa Gin cag Gin gct Ala tat Tyr.

230 t cc tcC Ser gg t Gly atg Met gcg Ala t tc Phe gca Ala atg Met gat Asp gca Ala atg Met 170 atg Met atc Ile att Ile ggc Gly gca ggg Gly gtc Val ctg Leu tat Tyr atg Met cc t Pro gtg Val1 ccg Pro cag Gin 155 cgC Arg gcg Ala atc Ilie tat Tyr gtC Val1 235 gac cgg Arg tcg Ser gga Gly cca Pro aac Asn 95 aat Asn cgt Arg ttc Phe atg Met ggt Gly 175 atc Ile at t Ile tgg Trp acc Thr agg acc Thr ggc Gly aac Asn ttC Phe gaa Giu acc Thr C cg Pro 130 g tg Val1 tac Tyr gcg Ala gtg Val att Ile 210 ctg Leu atg Met gtc ctg Leu gcg Ala gtg Val ttc Phe gtg Val1 ttg Leu 115 cat His ggC Gly ctg Leu cgt Arg gca Ala 195 gca Ala caa Gin ccg Pro tct gaa Giu tcg Ser ccg Pro gtC Val t ac Tyr ttC Phe 100 ac c Thr gag Giu acc Thr cgt Arg t tg Leu 180 atc Ile gac Asp gtt Val cga Arg gac WO 01/00843 WO 0100843PCT/IBOO/00923 Ser Arg Asn Ala Ala 250 aag Lys Asp Gly Asp Asp Arg 255 gca Ala Ala Val Val Ser Asp Leu 260 cag ttg ggt Gln Leu Gly ttc ttc acc Phe Phe Thr 280 tcc Ser 265 gcc Ala cta acc ttc Leu Thr Phe atc Ile 270 ctg atc ccc Leu Ile Pro atc gtg gtg Ile Val Val 275 931 979 ttc ggt gtc Phe Gly Val cct Pro 285 gcc Ala ggc caa 1027 Gly Gln 295 ttc tct 1075 Phe Ser 310 cgt gtg 1123 Arg Val gcc ggc 1171 Ala Gly ctg tcg 1219 Leu Ser ggt ttc 1267 Gly Phe 375 aac aaa 1315 Asn Lys 390 tgg gcg 1363 Trp Ala ggg tgg 1411 Gly Tr-p agc tcc 1459 Ser Ser ttc gat gcc aac Phe Asp Ala Asn gct ttc acg ctg Ala Phe Thr Leu 315 ttt tat gcg cgt Phe Tyr Ala Arg 330 gcc Ala 300 att gcc aat ggc Ile Ala Asn Gly aac atc ctt ggt Asn Ile Leu Gly 305 ctt ttt gcc tac Leu Phe Ala Tyr 290 tgg act ctg agc Trp Thr Leu Ser ctg cta cat ctg Leu Leu His Leu 325 att cct tac gct ttg Ile Pro Tyr Ala Leu 320 gaa gag gtc tgg ac Glu Glu Val Trp Thr 335 aag gtc gtg ctt tcc Lys Val Val Leu Ser gtg Val cca acc ttc atc atc Pro Thr Phe Ile Ile 340 ctg ttg gca ccg ctg Leu Leu Ala Pro Leu atc acc Ile Thr 345 agc tcc Ser Ser 360 gcc acc Ala Thr 350 ccg gag cgt gtg gtg Pro Glu Arg Val Val gtg ctt ctt ggt Val Leu Leu Gly 370 355 gcg gcc aac Ala Ala Asn agt ttc atc acc ggc Ser Phe Ile Thr Gly 380 ctc ggc ctg ttg ggt Leu Gly Leu Leu Gly 395 365 gcg Ala atg Met gtc atc ggc gcg tat ctg ttg cgc Val Ile Gly Ala Tyr Leu Leu Arg 385 cgc tct ttg gct aaa acc tcc ctg Arg Ser Leu Ala Lys Thr Ser Leu 400 ttg ggc tct Leu Gly Ser 410 ctg att caa Leu Ile Gln gcg gcg gtt ggt gca Ala Ala Val Gly Ala 415 gcc gtc gtg ggc gat Ala Val Val Gly Asp 430 ttg ttg aac ctg gct Leu Leu Asn Leu Ala gca Ala 405 gca gca tgg gcg ttg Ala Ala Trp Ala Leu 420 425 gta ggc Val Gly 440 ttc ttg ctg ggc act cta Phe Leu Leu Gly Thr Leu 435 gtg ttg ggt gtc ttc ttc Val Leu Gly Val Phe Phe 450 tac 445 WO 01/00843 atc ttc gt 1507 Ile Phe Va 455 caa aac tt 1555 Gin Asn Le 470 att cgc cc 1603 Ile Arg Pr cag gat tt 1651 Gin Asp Ph cct gtg cc 1699 Pro Val Pr 52 gtt ccc gg 1747 Val Pro Gi 535 cac ggc gg 1795 His Gly Gi 550 acc ggc aa 1843 Thr Gly Ly cca ttt gc 1891 Pro Phe Al gtg cag cg 1939 Vai Gin Ar gcc ccc aa 1987 Ala Pro As 615 gcc gat tg 2035 Ala Asp Tr 630 atc Ile gca Ala 475 aag Lys cag Gin atg Met gtc Val1 Gly 555 gcg Ala tct Ser aag Lys tcc Ser ggc Gly 635 gtg Val 460 ctg Leu atc Ile c tg Leu tcc Ser ggc Giy 540 gcg Ala ctg Leu tcg S er aag Lys gag Glu 620 tcc Ser tca Ser cgc Arg ttg Leu gcg Ala 510 ggt G ly ggt Gly ttc Phe ttt Phe gcc Ala 590 gcc Ala tac Tyr ttg Leu cgt Arg atc Ile gat Asp 495 cca Pro att Ile cgt Arg tgg Trp, gtg Val1 575 gca Ala agc Ser cgc Arg agc Ser tc t Ser cca Pro 480 gtc Val1 agc Ser gtc Val t tc Phe cag Gin 560 ga t Asp gcg Ala t tg Leu aac Asn gcc Ala 640 ccg Pro agc Ser gtc Val1 gca Ala 515 cct Pro ctc Leu gag Glu ggc Gly gcc Ala 595 ttg Leu ctc Leu gaa Glu PCTIBOO/00923 gag gtc Giu Val cgg ttt Arg Phe 485 tcc gag Ser Glu 500 gca acc Ala Thr cgc ctg Arg Leu gcc gat Ala Asp atc gcc Ile Ala 565 aac gcc Asn Ala 580 tac gag Tyr Glu gcg gta Ala Val att gtg Ile Val tcc ggt Ser Gly 645 WO 01/00843 WO 0100843PCT/IiB00I00923 gcc gat 2083 Ala Asp atc ggc 2131 Ile Gly tgc cga 2179 Cys Arg gcg att 2227 Ala Ile 695 tcg gcc 2275 Ser Ala 710 aag gca 2323 Lys Ala gat tac 2371 Asp Tyr gag gaa 2419 Glu Glu gcg aca 2467 Ala Thr 775 gca gcc 2515 Ala Ala 790 ggt acc 2563 Gly Thr tcc ccg 2611 Ser Pro ccc cgc gcc Pro Arg Ala 650 gag gcc cac Glu Ala His gcc gcg ttc gcg ctc Ala Ala Phe Ala Leu 655 gag atg ggt atc ccg Glu Met Gly Ile Pro gcg gaa cta act gaa acc Ala Glu Leu Thr Glu Thr 660 gcc ggc ttg gac aac aag Ala Gly Leu Asp Asn Lys 675 665 att cgt Ile Arg atc aac acc gac Ile Asn Thr Asp 685 cat gcc gtc ctc gcc ttg ccg His Ala Val Leu Ala Leu Pro 690 680 ttg ccc gat gcc tca Leu Pro Asp Ala Ser 700 gcc gag atg ctt atc Ala Glu Met Leu Ile gag ctc cgc gac Glu Leu Arg Asp gac gcg acc ctc Asp Ala Thr Leu 720 cag ggg cta gct Gln Gly Leu Ala gcc Ala 705 aag tcc ctg gcc Lys Ser Leu Ala atg gtc act Met Val Thr 730 715 gaa Glu gct ccc agc gac gtc Ala Pro Ser Asp Val 725 aca gaa gac aat ccc Thr Glu Asp Asn Pro 740 gcc Ala 735 gca tca Ala Ser 745 cca acc Pro Thr ctt gcc atg gcg Leu Ala Met Ala cac ctt gtg gtg His Leu Val Val 765 ggt ttc ggt gcc Gly Phe Gly Ala atg cgc acc tgc gga ctg ttc acc Met Arg Thr Cys Gly Leu Phe Thr 750 755 aag aag gaa aag aca cca aag cct Lys Lys Glu Lys Thr Pro Lys Pro 770 tcc gac tac 8CC gtc aag ggc atg Ser Asp Tyr Thr Val Lys Gly Met 785 760 cgt Arg gat Asp 780 atc gcc gct gtg Ile Ala Ala Val 795 gcg ttc ctc acc Ala Phe Leu Thr 810 ttg gcc tct gtt Leu Ala Ser Val 825 gtg Val atc atc ttg Ile Ile Leu agc ttc ttc ggc Ser Phe Phe Gly 815 gaa gcc acc acc Glu Ala Thr Thr 830 gtt tcc ctg gtg gcc gcc Val Ser Leu Val Ala Ala 800 805 agc agc acc aac gaa caa Ser Ser Thr Asn Glu Gin 820 tct gca aca cca gaa cct Ser Ala Thr Pro Glu Pro 835 WO 01/00843 WO 0100843PCT/IBOO/00923 gtg ggg 2659 Val Gly gac ggt 2707 Asp Gly 855 acc gca 2755 Thr Ala 870 acg cct 2803 Thr Pro gac agc 2851 Asp Ser tca cca 2899 Ser Pro tcc ggc 2947 Ser Gly 935 ggt cag 2995 Giy Gin 950 caa acc 3043 Gin Thr cca Pro 840 gca Ala tgg Trp ccg gtc tac ctg gat Pro Val Tyr Leu Asp 845 gga aca gat gtc acc Gly Thr Asp Val Thr 860 acc tcc acc ggc ggc Thr Ser Thr Gly Gly ctg gat caa gcc cgc acg tgg gat Leu Asp Gin Ala Arg Thr T-p, Asp 850 gac gtc acc gac ggc aac acc tcc Asp Val Thr Asp Gly Asn Thr Ser 865 gac ggc ctc cta gtt gac ctg tcc Asp Gly Leu Leu Val Asp Leu Ser 880 885 atc ttg acc acc ggc acc ggc tcc Ile Leu Thr Thr Gly Thr Gly Ser 895 900 aag atc tac gca ttc aac gac gcc Lys Ile Tyr Ala Phe Asn Asp Ala 875 gcc cgc ctc gac Ala Arg Leu Asp cgc gtc Arg Val aac gtg Asn Val 905 cac tcc His Ser tcg acc gtg Ser Thr Val 910 915 ctg tcg gaa ggc Leu Ser Giu Gly 925 atc gag atc ggc acc gtg gat tat Ile Giu Ile Gly Thr Vai Asp Tyr 930 arc cgc gat tcc tcc aag ctt ccg Ile Arg Asp Ser Ser Lys Leu-Pro cgc agt ctc agc Arg Ser Leu Ser gtg gaa tcc gtg Val Giu Ser Val cac His 940 agc Ser gtg att ctg gtc gat Val Ile Leu Val Asp 960 cca cag atg cag atc Pro Gin Met Gin Ile 975 gag gtt cgt tcc tca Glu Val Arg Ser Ser 965 gct gaa gta caa ctt Ala Giu Val Gin Leu 980 tca gac acc Ser Asp Thr 970 gtt ggt tgg 3075 Val Gly Trp taaattacgc gtttgtgatt gac <210> 594 <211> 984 <212> PRT <213> Cory-nebacteriun giutanicun <400> 594 Val Leu Gly Ala Val Leu Thr Ser Leu Val Ile Pro Val Leu Thr Arg 1 5 10 Ala Glu Lys Giu Asp Ala Asp Gly Gly Ser Gly Phe Phe Arg Arg Leu WO 01/00843 PCT/IB00/00923 25 Leu Thr Leu Ser Val Thr Leu Leu Gly Gly Val Thr Ile Leu Ser Ile 40 Ile Gly Ala Pro Leu Leu Thr Arg Met Met Leu Ser Ser Glu Gly Gin 55 Val Asn Val Val Met Ser Thr Ala Phe Ala Tyr Trp Leu Leu Pro Gin 70 75 Ile Phe Phe Tyr Gly Leu Phe Ala Leu Phe Met Ala Val Leu Asn Thr 90 Arg Glu Val Phe Lys Pro Gly Ala Trp Ala Pro Val Val Asn Asn Val 100 105 110 Ile Thr Leu Thr Val Leu Gly Val Tyr Met Val Leu Pro Ala Arg Leu.

115 120 125 His Pro His Glu Gin Val Gly Ile Phe Asp Pro Gin Ile Ile Phe Leu 130 135 140 Gly Val Gly Thr Thr Leu Gly Val Val Ala Gin Cys Leu Ile Met Ile 145 150 155 160 Pro Tyr Leu Arg Arg Ala Gly Ile Asp Met Arg Pro Leu Trp Gly Ile 165 170 175 Asp Ala Arg Leu Lys Gin Phe Gly Gly Met Ala Met Ala Ile Ile Val 180 1p5 190 Tyr Val Ala I-le Ser Gin Phe Gly Tyr Ile Ile Thr Thr Arg Ile Ala 195 200 205 Ser Ile Ala Asp Asp Ala Ala Pro Phe Ile Tyr Gin Gin His Trp Met 210 215 220 Leu Leu Gin Val Pro Tyr Gly Ile Ile Gly Val Thr Leu Leu Thr Ala 225 230 235 240 Ile Met Pro Arg Leu Ser Arg Asn Ala Ala Asp Gly Asp Asp Arg Ala 245 250 255 Val Val Ser Asp Leu Gin Leu Gly Ser Lys Leu Thr Phe Ile Ala Leu 260 265 270 Ile Pro Ile Val Val Phe Phe Thr Ala Phe Gly Val Pro Ile Ala Asn 275 280 285 Gly Leu Phe Ala Tyr Gly Gln Phe Asp Ala Asn Ala Ala Asn Ile Leu 290 295 300 Gly Trp Thr Leu Ser Phe Ser Ala Phe Thr Leu Ile Pro Tyr Ala Leu 305 310 315 320 Val Leu Leu His Leu Arg Val Phe Tyr Ala Arg Glu Glu Val Trp Thr 325 330 335 Pro Thr Phe Ile Ile Ala Gly Ile Thr Ala Thr Lys Val Val Leu Ser 340 345 350 WO 01/00843 WO 0100843PCT/IBOO/00923 Leu Leu Ala 385 Ala Ala Leu Leu Gly 465 Gly Gly Glu Arg Arg 545 Ala Thr Gly Gly Gly 625 Val Glu Pro Ala Leu Ser Ala 420 Thr Phe Glu Arg Ser 500 Ala Arg Ala Ile Asn 580 Tyr Ala Ile Ser Glu 660 Ser Phe 375 Lys Ala Trp Ser Phe 455 Asn Arg Asp Val1 Pro 535 Gly Gly Phe Gln Pro 615 Asp Asp Gly Ser 360 Ser Leu Leu Leu Val1 440 Val Leu Pro Phe Pro 520 Gly Gly Lys Ala Arg 600 Asn Trp Pro Glu Ser Phe Gly Gly Ile 425 Gly Thr Gly Asn Ser 505 Pro Ala Val Glu Pro 585 Arg I le Val1 Arg Ala 665 Val1 Val1 Arg Gly Gly 430 Leu Ser Arg Leu Ala 510 Gly Gly Phe Phe Ala 590 Ala Tyr Leu Ala Ile 670 Val1 Ile Ser Ala 415 Asp Ala Arg Ile Asp 495 Pro Ile Arg Trp Val 575 Ala Ser Arg Ser Leu 655 Pro Leu Gly Leu 400 Ala Phe Val Ser Pro 480 Val Ser Val1 Phe Gln 560 Asp Ala Leu Asn Ala 640 Ala Ala WO 01/00843 Gly Leu Asp 675 Val Leu Ala 690 Ala Lys Ser 705 Ala Pro Ser Thr Glu Asp Cys Gly Leu 755 Lys Thr Pro 770 Thr Val Lys 785 Ser Leu Val Ser Thr Asn Ala Thr Pro 835 Ala Arg Thr 850 Asp Gly Asn 865 Leu Val Asp Thr Gly Thr Ala Phe Asn 915 Gly Thr Val 930 Ser Ser Lys 945 Glu Val Arg Ala Glu Val PCT/iBOO/00923 Asn Leu Leu Asp Asn 740 Phe Lys Gly Ala Glu 820 Glu Trp Thr Leu Gly 900 Asp Asp Leu Ser Gln 980 Lys Pro Ala Val 725 Pro Thr Pro Met Al a 805 Gln Pro Asp Ser Ser 885 Ser Ala Tyr Pro Ser 965 Leu Ile 680 Leu Ala Met Ala Pro 760 Arg I le Ala Leu Pro 840 Ala Trp Ala Asn His 920 Arg Val1 Ser Trp Arg Pro Glu Val1 Ser 745 Thr Asp Ala Phe Ala 825 Pro Gly Thr Arg Val 905 Ser Ser Glu Asp I le Asp Met Thr 730 Leu His Gly Ala Leu 810 Ser Val1 Thr Ser Leu 890 Thr Leu Leu Ser Thr 970 Thr Ser 700 I le Ala Met Val Gly 780 Val Ser Glu Leu Val 860 Gly Arg Thr Glu His 940 Val Pro Asp 685 Glu Asp Gln Ala Val1 765 Ala Ile Phe Ala Asp 845 Thr Gly Val Val1 Gly 925 Ser Ile Gln Ala Asp Leu 720 Ala Thr Glu Tyr Val1 800 Ser Ser Gln Thr Leu 880 Thr Tyr Ile Asp Asp 960 Ile WO 01/00843 WO 0100843PCTAEBOO/00923 <210> 595 <211> 915 <212> DNA <213> Corynebacterium glutamicumt <220> <221> <222> <223>

CDS

(101)..(892) RXA0 1807 <400> 595 gctcaccgag ctggacacca agctccgcgc agtgoaggaa gaacacggcg agctggaaat gcagtggctg gaactcggcg aggaaatcga gggotagtto atg ccg tog goa Met Pro Ser Ala ggc Gly gag gag att tta Giu Giu Ile Leu cag cgc gca cag ctg gag ttt gat cag Gin Arg Ala Gin Leu Giu Phe Asp Gin cgc ogc Arg Arg gcc gat gtg Ala Asp Val cto agt gct Leu Ser Ala gtg Val atg ato ggc agc Met Ile Gly Ser gtg gtt tat ggt Val Val Tyr Gly too gtg ggg Ser Val Gly ogo gtg gtc Arg Val Val gcc att cog gtg Ala Ilie Pro Val cao aao gaa ggo His Asn Giu Gly got gto Ala Val 000 aoo gtg gtg tta agt too atg oog Pro Thr Val Val Leu Ser Ser Met Pro 60 ogt Arg tat goa agt tot Tyr Ala Ser Ser ogo cag oog atg tog gac oaa tgg oto Arg Gin Pro Met Ser Asp Gin Trp, Leu gao gog otg caa Asp Ala Leu Gin otg gtg gat otg Leu Val Asp Leu att ato gat gag Ile Ile Asp Giu too aco att tco Ser Thr Ile Ser aoo ggo Thr Gly 100 tat ttt aoo Tyr Phe Thr aaa ato ogo Lys Ile Arg 120 too Ser 105 got tot cag gtg Ala Ser Gin Val gtg gto got gog Val Val Ala Ala tgg otg cag Trp Leu Gin 115 gat 000 ato Asp Pro Ile gaa aco oat ocg oat gtg ogo ato gtg Giu Thr His Pro His Vai Arg Ile Val 125 g tg Val1 130 atg ggg Met Gly 135 gac agt gac gtg gga att tat gtc gc Asp Ser Asp Val Gly Ilie Tyr Val Ala 140 gao Asp 145 gag atc goa acc Giu Ilie Ala Thr ato tgo oag gao Ile Cys Gin Asp tgo cot otg got CYs Pro Leu Ala gga ato att coo Gly Ile Ile Pro gct ttc gag oto Ala Phe Giu Leu too Ser 170 oac atg gtt ggc His Met Val Gly ggc gat cog ogo Gly Asp Pro Arg tog ctg Ser Leu 180 wo 01/008 ctc ggc Leu Gly gtg ggc Val Gly atc gcc Ilie Ala 215 tac gcc Tyr Ala 230 gac gcc Asp Ala aaa gcg Lys Ala :43 ccg ttt ggc gag t99 atc atc at Pro Phe Gly Glu Trp Ile Ile I) 185 190 acc acc gtc acc cgc atc gtc ac Thr Thr Val Thr Arg Ile Val T1 200 205 tcc gcc acc gtc gat acc acg gc Ser Ala Thr Val Asp Thr Thr A) 220 gca gca tta atc gcc gcc ctg cz Ala Ala Leu Ilie Ala Ala Leu Hi 235 gcc agc cac gca tcc aac acc gt Ala Ser His Ala Ser Asn Thr Vz 250 2 ctt taggtttcgt ccgtctctga cag Leu ir Lt PCT/IB00100923 ia act 691 Lu Thr ig gaa 739 In Giu Ic gtc 787 3p Val atc 835 au Ile 245 ag acc 883 Ln Thr gca ggc Ala Gly ttt tcg Phe Ser ggc ctg Gly Leu 915 <210> 596 <211> 264 <212> PRT <213> Corynebacterium giutamicum <400> 596 Met Pro Ser Ala 1 Phe Asp Gin Arg Tyr Gly Ser Val Gly Leu Arg Val Arg Tyr Ala Ser Asp Ala Leu Gin Thr Ile Ser Thr 100 Ala Ala Trp Leu 115 Val Val Asp Pro Vi0 Glu Giu Ilie Leu Giu Gin Arg Ala Gin Leu Giu Asp Ser Val1 Arg Val1 Phe Ile Gly 135 Val1 Ala Pro Gin

ASP

Thr Arg 120 Asp Val Ala Thr Pro Leu Ser 105 Giu Ser Met Ilie Val met Gly Ala Thr Asp Val Giu Pro Ala Ser Val Ile Ala Glu Ilie Ala Thr Ala Ile Cys Gin Asp Leu Cys Pro Leu Ala Thr WO 01/00843 Gly Asp Ser Asp Gly 225 Asp Ala Glu Pro Thr 200 Ser Ala Ala Leu Leu Phe 185 Thr Ala Ala Ser Ser 170 Gly Val1 Thr Leu His 250 Val1 Ile Ile 205 Thr Al a Asn PCTIBOO/00923 Ser Gly 175 Ile Thr Thr Arg Ala Lys Hius Lys 240 Val Cys 255 <210> 597 <211> 1461 <212> DNA <213> Corynebacteriun giutamicum <22 0> <221> CDS <222> (101)..(1438) <223> RXN02754 <400> 597 attatgaagc catcggagtt ggtgtggcct acaagggtga agttcactgt agctcccgct gattccgtag aatcaacaga tct Ser gag Glu ccc Pro tac Tyr ttt Phe cgt Arg cca acg Ser Thr ggt gtc Gly Val gta ttc Val Phe acc ctg Thr Leu cct gag Phe Glu gtc gca Val Ala aca gag Thr Glu gaa tac Giu Tyr cgt tca Arg Ser gca gcg Ala Ala ttt agc Phe Ser 45 aca gca Thr Ala 60 caa ctc Gin Leu cgc aac Arg Asn aca Thr ctc Leu cgc Arg cga Arg gcc Ala tac Tyr 851 tcatgcgtgg atagtggtgg gtg aat acc aat ccg Val Asn Thr Asn Pro 1 ctt act gat aaa. tat Leu Thr Asp Lys Tyr ggt tct gca gaa cgc Gly Ser Ala Glu Arg ccc aac gag cgc cga Pro Asn Glu Arg Arg aag gcg att cgt gac Lys Ala Ile Arg Asp gac ttt tta gac gac Asp Phe Leu Asp Asp acc ggc caa gtt gat Thr Gly Gin Val Asp WO 01/00843 ggc tac Gly Tyr gtg cgt Val Arg tcc atc Ser Ile 135 gtc acc Vai Thr 150 acc cac Thr His gga ttc Gly Phe cca gca Pro Ala gat gac Asp Asp 215 tcc ctc Ser Leu 230 caa ggt Gin Gly gta cgt Val Arg aag cag Lys Gin tcc gac 1027 Ser Asp 295 gac gtc 1075 Asp Vai 310 ttc Phe 110 gtC Vali gtc Val1 atc Ile gca Ala gag Glu 190 gca Ala qca Ala c tg Leu gtt Val ggt Gly 270 cac His gcg Aila gtt Val1 ccg Pro atc Ile qct Ala atc Ile tcc Ser 175 gcg Ala tgg Trp gct Ala gta Val gca Ala 255 gtg Val aac Asn gg t Gly gtC Val1 cag Gin cta Leu tcc Ser gaa Giu 160 cgc Arg gcC Aia act Thr t tc Phe gat Asp 240 ggt Gly ctt Leu acc Thr ctt Leu aca Thr 320 PCTIiBOOIOO923 100 ctg act 451 Leu Thr att ttg 499 Ile Leu cgc atg 547 Arg Met agg cgc 595 Arg Arg 165 ctc gct 643 Leu Ala 180 gga att 691 Gly Ile atc aac 739 Ile Asn gtt gaa 787 Val Giu atc acc 835 Ile Thr 245 ggt ggc 883 Gly Gly 260 gtc cgc 931 Val Arg gtc tcc 979 Val Ser cca gtt Pro Vai gca cca Ala Pro 325 WO 01/00843 WO 0100843PCT/EBOO/00923 acc gct 1123 Thr Ala gcc aag 1171 Ala Lys gtg cgc 1219 Val Arg cca ttc 1267 Pro Phe 375 ctg acc 1315 Leu Thr 390 act ttg 1363 Thr Leu cca tgg 1411 Pro Trp cgt ttc 1458 ggc ctc gtg Gly Leu Val 330 cgt tcc cga Arg Ser Arg tac aag atc Tyr Lys Ile aac aag-gaa.

Asn Lys Glu ggg gaa Gly Glu 335 agc tac Ser Tyr gtt gcc ggt cac cct gtg Val Ala Gly His Pro Val 340 ggt ggt ggc aag aag gct Gly Gly Gly Lys Lys Ala 355 atc gaa gaa atc gtc tac Ile Giu Giu Ile Val Tyr 345 acc cac Thr His cgc aag tcc Arg Lys Ser gaa gca cca Giu Ala Pro ggt Gly 365 350 acc Thr gca Ala gcc Ala 380 atc cca ttg atg Ile Pro Leu Met 395 gaa gat tcc cga Giu Asp Ser Arg 410 cgc Arg gcg Ala gat act gga aag Asp Thr Gly Lys gac ggt gaa atc Asp Gly Giu Ile 400 tat ttg gcc aag Tyr Leu Ala Lys gtt cca ggt ttg cct Val Pro Gly Leu Pro 405 caa ttg gtc tct tta Gin Leu Val Ser Leu 420 cct gtt ttg cac act Pro Val Leu His Thr 435 ctc Leu 385 370 gac act ttg agc Asp Thr Leu Ser 415 gaa ggc Glu Giy 425 gtg ggt ctt gca ctg tct cgc Leu Ala Leu Ser Arg 430 ttc ccg ccg gcc gct gat Asp gag Giu tagacaattc ggtctcacca Arg Phe Val Gly Phe Pro Pro Ala Ala aac 1461 <210> 598 <211> 446 <212> PRT <213> Corynebacteriun glutamicum <400> 598 Val Asn Thr Asn Pro Ser Giu Phe Ser Ser Asn Arg 1 5 10 Leu Thr Asp Lys Tyr Glu Leu Thr Met Leu Gin Ala 25 Gly Ser Ala Glu Arg Pro Ser Thr Phe Giu Val Phe Pro Asn Giu Arg Arg Tyr Gly Val Vai Ala Gly Thr 55 Ser Thr Ala Leu Ala Leu Ala Asp Arg Arg Leu Arg Val Leu WO 01/00843 WO 0100843PCTJIBOO/00923 Lys Asp Thr Ser Glu Ala 145 Met Ala Tyr Leu Lys 225 Thr Pro Ala Lys Arg 305 Gly Ala Gly Glu Ala Phe Gly Pro Thr 130 Ala Gly Ala Arg Leu 210 Ala Tyr Asp Arg I le 290 Gly Ser Gly Gly Glu 370 Ile Arg Asp Leu Asp Asp Gin Val Asp 100 Leu Leu Thr 115 Val Ilie Leu Ala Arg Met Ser Arg Arg 165 Tyr Leu Ala 180 Tyr Gly Ilie 195 His Ilie Asn Gin Val Giu Asp Ilie Thr 245 Leu Gly Gly 260 Lys Val Arg 275 Val Val Ser Giu Pro Val Gly Ala Pro 325 His Pro Val 340 Lys Lys Ala 355 Ile Val Tyr Phe Val Phe Thr Glu Glu Gin Leu Ala Asp Leu 70 Arg Gly Val Ser Val1 150 Thr Gly Pro Asp Ser 230 Gin Val Lys Ser Asp 310 Thr Aia Val1 Pro 75 Thr Tyr Arg I le 135 Thr His Phe Al a Asp 215 Leu Gly Arg Gln Asp 295 Val1 Ala Lys Arg Phe 375 Leu Arg Gly 120 Met Ala Glu Ser Ser 200 Gly Gly Val Ile Leu 280 Leu Phe Gly Arg Thr 360 Asn Giu Giu 105 Thr Asn Ala Tyr Thr 185 Gly Thr Val Ala Asp 265

ASP

Asp Gly Leu Ser 345 His Ala Tyr 90 Gly Phe Ala Asp Ser 170 Thr Thr Pro Asp Thr 250 Ser Asp Giu Val Val 330 Arg Arg Glu Leu Glu Al a

ASP

Gly 155 Ala Ser Ser Asn Thr 235 Ala Gly Leu Phe Gly 315 Tyr Asn Lys Ala Arg I le Glu Ser 140 Arg Val1 Asn Ala Glu *2 Thr I le Asp Asn Ala 300 Thr Lys Lys Ser Pro 380 Asn Tyr Tyr Phe 110 Cys Val 125 Ala Val Pro Ile Thr Ala Leu Glu 190 His Ala 205 Ala Ala Leu Leu Glu Val Leu Gly 270 Ala His 285 Ile Ala Ser Val Ile Gly Glu Ser 350 Gly Thr 365 Asp Thr Arg Pro Ile Ala Ile S er 175 Ala Trp, Ala Val Ala 255 Val Asn Gly Val1 Giu 335 Tyr Ala Gly Phe Gln Leu Ser Glu 160 Arg Ala Thr Phe Asp 240 Gly Leu Thr Leu Thr 320 Val1 Gly Ile Lys WO 01/00843 PCTJIBOO/00923 Leu Asp Thr Leu Ser Leu Thr Ile Pro Leu Met Arg Asp Gly Giu Ile 385 390 395 400 Vai Pro Gly Leu Pro Thr Leu Giu Asp Ser Arg Ala Tyr Leu Ala Lys 405 410 415 Gin Leu Val Ser Leu Pro Trp Giu Gly Leu Ala Leu Ser Arg Asp Giu 420 425 430 Pro Val Leu His Thr Arg Phe Val Gly Phe Pro Pro Ala Ala 435 440 445 <210> 599 <211> 871 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (101)..(871) <223> FRXA02405 <400> 599 attatgaagc catcggagtt ggtgtggcct acaagggtga tcatgcgtgg atagtggtgg agttcactgt agctcccgct gattccgtag aatcaacaga gtg aat acc aat ccg Val Asn Thr Asn Pro tc t Ser gag Giu ccc Pro tac Tyr ttt Phe cgt Arg ggc Gly gaa Giu ctg Leu tca Ser ggt Gly gta Val1 acc Thr tac Tyr ttc Phe acc Thr acg Thr gtc Val1 ttc Phe ctg Leu cgc Arg cgt Arg gca Ala ttt Phe aca Thr caa Gin cgc Arg atc Ile gct Ala gct Ala cgc Arg gtg Val1 gat Asp cga Arg ccg Pro at c Ile ctc Leu ga t Asp ctc Leu ctg Leu ctt Leu t tc Phe cag Gin cta Leu act gat Thr Asp tct gca Ser Ala aac gag Asn Giu gcg att Ala Ile ttt tta Phe Leu ggc caa Gly Gin cct* ctt Pro Leu 115 act gtc Thr Val 130 gtg cgt ggc acg ttt gca gaa tgc Val Arg Gly Thr Phe Ala Giu Cys WO 01/00843 WO 0100843PCTIBOOIOO923 tcc atc atg aat gca gat tct gcc gtc gct tcc gcc gct gcg cgc atg 547 Ser Ile Met Asn Ala Asp Ser Ala Val Ala Ser Ala Ala Ala Arg Met 135 140 145 gtc acc gca gct gat ggt cgc ccc atc atc gaa atg gga tcc agg cgc 595 Val Thr Ala Ala Asp Gly Arg Pro Ile Ile Glu Met Gly Ser Arg Arg 150 155 160 165 acc cac gaa tac tcg gca gtc acc gca tcc cgc gca gca tac ctc gct 643 Thr His Glu Tyr Ser Ala Vai Thr Ala Ser Arg Ala Ala Tyr Leu Ala 170 175 180 gga ttc tcc acc acc tcc aac ctc gag gcg gcc tac cgc tac gga att 691 Gly Phe Ser Thr Thr Ser Asn Leu Glu Ala Ala Tyr Arg Tyr Gly Ile 185 190 195 cca gca tcc gga acc tcc gcc cac gca tgg act ttg ctg cac atc aac 739 Pro Ala Ser Gly Thr Ser Ala His Ala Trp Thr Leu Leu His Ilie Asn 200 205 210 gat gac ggc acc ccc aac gaa gca gca gct ttc aaa gca cag gtt gaa 787 Asp Asp Gly Thr Pro Asn Glu Ala Ala Ala Phe Lys Ala Gin Val Giu 215 220 225 tcc ctc ggc gtg gac acc acc ttg ctg gta gat act tat gac atc acc 835 Ser Leu Gly Val Asp Thr Thr Leu Leu Val Asp Thr Tyr Asp Ilie Thr 230 235 240 245 caa ggt gtg gcc acc gcc att gaa gtt gca ggt cca 871 Gin Gly Val Ala Thr Ala Ile Giu Val Ala Gly Pro 250 255 <210> 600 <211> 257 <212> PRT <213> Corynebacteriun glutamicun <400> 600 Val Asn Thr Asn.Pro Ser Giu Phe Ser Ser Asn Arg Ser Thr Ala Leu 1 5 10 Leu Thr Asp Lys Tyr Giu Leu Thr Met Leu Gin Ala Ala Leu Ala Asp 25 Gly Ser Ala Giu Arg Pro Ser Thr Phe Glu Val Phe Ser Arg Arg Leu 40 Pro Asn Glu Arg Arg Tyr Gly Vai Val Ala Gly Thr Ala Arg Val Leu 55 Lys Ala Ilie Arg Asp Phe Vai Phe Thr Glu Giu Gin Leu Ala Asp Leu 70 75 Asp Phe Leu Asp Asp Arg Thr Leu Giu Tyr Leu Arg Asn Tyr Arg Phe 90 Thr Gly Gin Val Asp Gly Tyr Arg Giu Gly Giu Ile Tyr Phe Pro Gin 100 105 110 WO 01/00843 Ser Pro Leu 115 Giu Thr Val 130 Ala Ala Ala 145 Met Gly Ser Ala Ala Tyr Tyr Arg Tyr 195 Leu Leu His 210' Lys Ala Gin 225 Thr Tyr Asp Pro Leu Ile Arg Arg Leu 180 G ly Ile Val Ile Thr Leu Met Arg 165 Ala Ile Asn Glu Thr 245 Gly 120 Met Ala Giu Ser Ser 200 Gly Giy Val1 3 3

C

3 hr ~sn Lyr rhr fly Lhr Tal l.a :cc ;er It t al1 25 :ca ?ro ;tg Pal c t la Phe Ala Asp Ser 170 Thr Thr Pro Asp Thr 250 tcc Ser gac Asp acc Thr gcc Al a gtg Val1 Al a

ASP

Gly 155 Ala Ser Ser Asn Thr 235 Ala gac

ASP

gtc Val gc t Ala aag Lys cgc Arg 75 Giu Ser 140 Arg Vai Asn Ala Giu 220 Thr Ile ctg Leu ttt Phe ggc Gly cgt Arg acc Thr Cys 125 Ala Pro Thr Leu His 205 Ala Leu Giu gat Asp ggc Gly ctc Leu tcc S er cac His Val Val1 Ile Ala Glu 190 Ala Ala Leu Val1 gaa Glu gtt Val1 gtg Val1 cga Arg cgc Arg PCTIiBOO/00923 Ile Leu Ala Ser Ile Glu 160 Ser Arg 175 Ala Ala Trp Thr Ala Phe Val Asp 240 Ala Gly 255 <210> 601 <211> 509 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> <223> FRXA02754 <400> 601 ccc aca aac acc aag att gtg gtc Pro Thr Asn Thr Lys Ile Val Val 1 5 atc gcg ggt ctt cgc ggc gaa cca Ile Ala Gly Leu Arg Gly Glu Pro N tcc gtt gtc aca ggt tct ggc gca c Ser Val Val Thr Gly Ser Gly Ala 40 atc ggg gaa gtt gcc ggt cac cct Ile Gly Giu Val Ala Gly His Pro N 55 gaa agc tac ggt ggt ggc aag aag Giu Ser Tyr Gly Gly Gly Lys Lys 70 gcc Ala acc Thr aag Lys aag Lys t cc Ser WO 01/00843 WO 0100843PCTA0BO0/00923 ggt Gly gat Asp gac Asp tat Tyr tct Ser 145 gcc Ala gca atc gaa gaa atc gtc tz Ala Ile Glu Giu Ile Val T) gga aag ctc gac act ttg a5 Gly Lys Leu Asp Thr Leu S( 100V gaa atc gtt cca ggt ttg c( Giu Ile Val Pro Gly Leu P3 115 120 gcc aag caa ttg gtc tct tt Ala Lys Gin Leu Val Ser LE 135 gat gag cct gtt ttg cac ac Asp Glu Pro Val Leu His TI 150 tagacaattc ggtctcacca aac act ttg gaa Thr Leu Glu gcc Ala cca Pro gat Asp 125 ggc Gly ggt Gly gaa Glu ttg Leu 110 tcc Ser ctt Leu t tc Phe gca cca Ala Pro atg cgc Met Arg cga gcg Arg Ala gca ctg Ala Leu ccg ccg Pro Pro 160 <210> 602 <211> 162 <212> PRT <213> Corynebacterium glutamicun <400> 602 Pro Thr Asn Thr Lys Ile Val Val Ser 1 Ile Ser Ile Glu Gly Asp Asp Tyr Ser 145 Gly Ser Gly Gly Glu Asp Pro Leu Val1 150 Giu G ly His Lys Ile Thr Gly Val1 135 Leu Ser 10 Asp Thr Ala Val1 Pro Leu Thr Pro Arg Asp Gly Leu Ser His Ala Pro Asp 125 Gly Gly Glu Val1 Val1 Arg Arg Glu Leu 110 Ser Leu Phe Phe Gly Tyr Asn Lys Ala Met Arg Ala Pro WO 01/00843 WO 0100843PCTIIBOOIOO923 Ala Ala <210> 603 <211> 960 <212> DNA <213> Corynebacterium giutami cuxn <220> <221> <222> <223>

CDS

(101)..(937) RXA0 2112 <400> 603 tgaatccgtg gcggcaccgg cgcgagtcgc tttggaaagg atgctatctg ttgttccagc agctcctgtt actcctagct cctcgaagga tgcgtaattt atg act acc cat Met Thr Thr His gac cgc atc gtt Asp Arg Ile Val gca gcg tta tcc Ala Ala Leu Ser gat gcg cca tgg Asp Ala Pro Trp ggc gac Gly Asp att acc tcc Ilie Thr Ser gtt gtt gcc Val Val Ala act ttt atc cca Thr Phe Ilie Pro gga Gly tcg gcg cag ctg Ser Ala Gin Leu agc gcc aag Ser Ala Lys ctt tta gac Leu Leu Asp cgg gag cca ggt Arg Giu Pro Gly ttc agc ggg cag Phe Ser Gly Gin 211 259 307 355 gcc tcc Ala Ser ttc cgg ctc gtc Phe Arg Leu Val cct agg ata aac gca tcc ctt aag gtg Pro Arg Ile Asn Ala Ser Leu Lys Val gct gat ggt gac agc ttt gaa acc ggg gac atc cta gga aca att acc Ala Asp Gly Asp Ser Phe Giu Thr Gly Asp Ile Leu Gly Thr Ile Thr 75 80 ggc agt gct aga Gly Ser Ala Arg atc ctc cgt tca Ilie Leu Arg Ser cgc att gct ctc Arg Ile Ala Leu aac ttc Asn Phe 100 att cag agg Ile Gin Arg gag gtt aaa Giu Val Lys 120 acg Thr 105 tcc ggc atc gct Ser Gly Ile Ala aca Thr 110 ttg aca tcg tgc Leu Thr Ser Cys tat gtt gca Tyr Val Ala 115 aaa acc aca Lys Thr Thr ggc acc aaa gcc Gly Thr Lys Ala att gtt gat acc Ile Val Asp Thr cgg Arg 130 451 499 547 595 ccc ggc Pro Gly 135 ctg cgc atc att gaa cgc caa gct gtc Leu Arg Ilie Ile Giu Arg Gin Ala Val cgt Arg 145 gac ggt ggc gga Asp Gly Gly Gly ttt Phe 150 aat cac cga gcc acc ttg tcc gat gct Asn His Arg Ala Thr Leu Ser Asp Ala 155 atg gtg aaa gat Met Val Lys Asp aac Asn 165 WO 01/00843 WO 0100843PCT/11BOO/00923 cat ctc gca gcc atc gca tcc cag ggg ctc agc atc act gaa gcg ctg 643 His Leu Ala Ala Ile Ala Ser Gin Gly Leu Ser Ile Thr Giu Ala Leu 170 175 180 tcg aat atg aaa gct aaa ctc ccc cac acc acc cat gtg gaa gtc gaa 691 Ser Asn Met Lys Ala Lys Leu Pro His Thr Thr His Val Glu Val Giu 185 190 195 gtt gat cat ata gag cag atc gaa cca gtt ctt gct gct ggt gtg gac 739 Vai Asp His Ilie Glu Gin Ilie Giu Pro Val Leu Ala Ala Gly Val Asp 200 205 210 acc atc atg ttg gat aat ttc acc att gat cag ctc atc gaa ggc gtt 787 Thr Ile Met Leu Asp Asn Phe Thr Ilie Asp Gin Leu Ile Glu Gly Val 215 220 225 gat ctc att ggt gga cgt gca ctg gtg gaa gca tct ggc gga gtc aac 835 Asp Leu Ilie Gly Giy Arg Ala Leu Val Giu Ala Ser Gly Giy Vai Asn 230 235 240 245 ctc aac acc gcg gga aag att gca tca acc ggt gtc gac gtc att tcc 883 Leu Asn Thr Ala Gly Lys Ilie Ala Ser Thr Giy Val Asp Val Ile Ser 250 255 260 gtt gga gcg ctt acc cat tct gtg cat gca ctt gac cta gga ctc gat 931 Val Giy Ala Leu Thr His Ser Val His Ala .Leu Asp Leu Gly Leu Asp 265 270 275 att ttc taatgctcta ccttgataat gca 960 Ile Phe <210> 604 <2i1> 279 <212> PRT <213> Corynebacteriun giutamicum <400> 604 Met Thr Thr His Ile Asp Arg Ile Vai Gly Ala Ala Leu Ser Glu Asp 1 5 10 Ala Pro Trp Gly Asp Ilie Thr Ser Asp Thr Phe Ile Pro Gly Ser Ala 25 Gin Leu Ser Ala Lys Val Val Ala Arg Giu Pro Gly Val Phe Ser Gly 40 Gin Ala Leu Leu Asp Ala Ser Phe Arg Leu Val Asp Pro Arg Ile Asn 55 Ala Ser Leu Lys Val Ala Asp Gly Asp Ser Phe Giu Th~r Gly Asp Ile 70 75 Leu Gly Thr Ilie Thr Gly Ser Ala Arg Ser Ile Leu Arg Ser Giu Arg 90 Ilie Ala Leu Asn Phe Ile Gin Arg Thr Ser Gly Ile Ala Thr Leu Thr 100 105 110 WO 01/00843 Ser Thr Arg 145 Met Ile His Ala Leu 225 Ser Val Asp Cys Arg 130 Asp Val1 Thr Val1 Ala 210 Ile Gly Asp Leu Val1 Gly 135 Asn Leu Asn Asp Ile 215 Leu Asn Gly Phe Lys 120 Leu His Al a Met His 200 Met Ile Thr Ala Gly Arg Arg Ala Lys 185 Ile Leu Gly Ala Leu 265 Ile Gin Asp Gly His 190 Pro Ile Val1 Ser His 270 PCTIiBOO/00923 Val Asp Ala Val Ala Val 160 Leu Ser 175 Thr Thr Val Leu Asp Gin Glu Ala 240 Thr Gly 255 Ala Leu <210> 605 <211> 1407 <212> DNA <213> Corynebacterium glutanicun <220> <221> CDS <222> (101)..(1384) <223> RXA02111 <400> 605 gcttgcggga acaccgcacc gcccacccaa actgttcaga gctcatztcca gcccaccgtt tagaagaaaa gaccccaatc acc cca tct gtc aac ctt gca ttg aaa aat gcc Thr Pro Ser Val Asn Leu Ala Leu Lys Asn Ala 15 gaa ctc aaa gac gga ccc tgg ttc ctc gac cag Glu Leu Lys Asp Gly Pro Trp Phe Leu Asp Gin 30 gtc tac ggc ccc ggc gcg tca caa aac gat ccg Val Tyr Gly Pro Gly Ala Ser Gin Asn Asp Pro 861 ttccaaagat aaattctgac atg acc acc tca atc 115 Met Thr Thr Ser Ile aat agc tgc aac agt Asn Ser Cys Asn Ser ccc gga atg ccg gat Pro Gly Met Pro Asp atc cct gcg cat gct Ile Pro Ala His Ala WO 01/00843 WO 0100843PCT/IBOO/00923 ccg cgc Pro Arg cag cag gtt ctc Gin Gin Val Leu gag gag tac cag Glu Giu Tyr Gin gca agr gat gac Ala Ser Asp Asp gaa Giu ctg cat cgt agg Leu His Arg Arg cgg gaa gcg aaa Arg Giu Ala Lys acc crg ggt gac Thr Leu Gly Asp gtg gtt arc cta Val Val Ile Leu cac ttc tac cag His Phe Tyr Gin cgc Arg gat gaa gtt arc Asp Giu Val Ile caa cac Gin His 100 gca gat ttt gtt ggt gac tct ttc Ala Asp Phe Vai Gly Asp Ser Phe 105 ctt gcc cgc gct gcc aaa acc Leu Ala Arg Ala Ala Lys Thr 115 cga ccc gag Arg Pro Giu 120 gcg gaa gcg at Ala Glu Ala Ile ttc tgc ggt gtg Phe Cys Gly Val cac His 130 ttc atg gct Phe Met Ala gaa acc Giu Thr 135 gct gat ctg rra tcc acg gat gaa caa Ala Asp Leu Leu Ser Thr Asp Glu Gin 140 tca Ser 145 grg atc ctc ccc Val Ile Leu Pro ctt gcc gca ggt Leu Ala Ala Gly tgc Cys 155 tcc atg gca gac Ser Met Ala Asp atg Met 160 gct gac ctt gat Ala Asp Leu Asp grc gaa gac rgc Val Giu Asp Cys rgg Trp 170 gag caa crc acc Giu Gin Leu Thr rca Ser 175 atr tat ggc gat Ile Tyr Gly Asp gac acc Asp Thr 180 ctg atc cct Leu Ilie Pro gtg ggt gag Val Gly Giu 200 acc tac arg aat Thr Tyr Met Asn tct gca gcg crc Ser Ala Ala Leu aaa ggt ttc Lys Gly Phe 195 gca cgt tcc Ala Arg Ser cac ggc gga at His Gly Gly Ile rgc acc tcc rca Cys Thr Ser Ser aat Asn 210 gra ttg Val Leu 215 gag tgg gcg ttt Giu Trp Ala Phe gaa Giu 220 cgc ggc caa cga Arg Gly Gin Arg gtc Val 225 ctg ttc ttc ccc Leu Phe Phe Pro ga t Asp 230 cag cac rtg ggt Gin His Leu Gly cga Arg 235 aac acc gcg aaa Asn Thr Ala Lys gcc Al a 240 atg ggc att ggg Met Gly Ile Gly gar caa atg ccc Asp Gin Met Pro ctg Leu 250 tgg aat ccc aac Trp, Asn Pro Asn cca ctg ggt ggc Pro Leu Gly Gly aac acc Asn Thr 260 gtt tcc gag Val Ser Glu tct gra cac Ser Val His 280 cra Leu 265 gaa aac gca aag Glu Asn Ala Lys ctg crc tgg cat Leu Leu Trp His ggt ttc tgc Gly Phe Cys 275 gcc cgc gcc Ala Arg Ala aag cgc ttt act Lys Arg Phe Thr gag cag arc aac Glu Gin Ile Asn WO 01/00843 WO 0100843PCT/BOO/00923 gag tac 1027 Giu Tyr 295 gtt gtt 1075 Val Val 310 gcc att 1123 Ala Ile atc aac 1171 Ile Asn ttc tgc 1219 Phe Cys cac cct 1267 His Pro 375 gtg att 1315 Val Ile 390 gtc gct 1363 Val Ala cct agc 1407 Pro Ser ccc gac gtt cac gtc Pro ASP Val His Val 300 gac gcc gcc gac tca Asp Ala Ala Asp Ser 315 caa gca gca ccg gca Gin Ala Ala Pro Ala 330 ttg gtt cag cgc ctg Leu Val Gin Arg Leu 345 ctc gac cct gtc atc Leu Asp Pro Val Ile 360 ggt tac ctg gcc tgg Gly Tyr Leu Ala Trp 380 atc gtg cac cct gaa tcc ccc atg cca Ile Val His Pro Giu Ser Pro Met Pro 305 tcc gga tcc act gac ttc att gtg aaa Ser Gly Ser Thr Asp Phe Ile Val Lys gga tct Gly Ser gca gcc Ala Aia 350 tgc cca Cys Pro 365 gca ctt Ala Leu acc Thr 335 gcg atc ggc acc Ala Ile Gly Thr 340 cag tac ccg cag cac acc atc Gin Tyr Pro Gin His Thr Ile 355 tgc tcc acc atg tat cgc att Cys Ser Thr Met Tyr Arg Ile 370 gag gag ttg gtg gct gga aac Giu Giu Leu Val Ala Gly Asn 385 aac cag att tct Asn Gin Ile Ser 395 ttg gaa agg atg Leu Giu Arg Met 410 tcc tcg aag gat Ser Ser Lys Asp 425 gtc tct gaa tcc gtg Val Ser Giu Ser Val 400 cta tct gtt gtt cca Leu Ser Val Val Pro 415 gcg gca ccg gcg cga Ala Ala Pro Ala Arg 405 gca gct cct gtt act Ala Ala Pro Val Thr 420 taatttatga ctacccatat tga <210> 606 <211> 428 <212> PRT <213> Corynebacterium giutamicum <400> 606 Met Thr Thr Ser Ilie Thr Pro Ser Val Asn Leu Ala Leu Lys Asn Ala 1 5 10 Asn Ser Cys Asn Ser Giu Leu Lys Asp Gly Pro Trp Phe Leu Asp Gin 25 Pro Gly Met Pro Asp Val Tyr Gly Pro Gly Ala Ser Gin Asn Asp Pro 40 WO 01/00843 WO 0100843PCTIiBOO/00923 Ile Arg Thr Glu Arg Val1 Ser 145 Ala Tyr Ala Ser Val 225 Met Leu Trp Asn Glu 305 Asp Ala Pro Ala His Ala Asp Asp Gin 100 Lys Met Leu Asp Asp 180 Gly Arg Phe Gly Asn 260 Phe Arg Met Val Thr 340 Thr Asp Lys His Thr Ala Pro Ser 165 Thr Phe Ser Pro Ile 245 Thr Cys Al a Pro Lys 325 Giu Ile Pro Glu 70 Val1 Ala Arg Giu Asn 150 Val1 Leu Val Val Asp 230 Asp Val1 Ser Glu Val1 310 Ala Ile Phe Gin Val Leu Arg Arg Ile Leu Gly His Val Gly Asp 105 Ala Glu Ala Asp Leu Leu Ala Gly Cys 155 Cys Trp Giu 170 Val Thr Tyr 185 His Gly Gly Trp Ala Phe Leu Gly Arg 235 Pro Leu Trp 250 Leu Glu Asn 265 Lys Arg Phe Asp Val His Pro Arg Phe Ser Ile Ser 140 Ser Gin Met Ile Gi u 220 Asn Asn Ala Thr Val1 300 Ser Ala Leu Ile Giu Giu Tyr Phe Val1 125 Thr Met Leu Asn Val1 205 Arg Thr Pro Lys Val 285 Ile Ser Gly Ala Cys 365 Giu Ala Gin Gin 110 Phe Asp Ala Thr Ser 190 Cys Gly Ala Asn Val 270 Glu Val1 Gly Ser Al a 350 Pro WO 01/00843 Thr Met Tyr 370 Leu Val Ala 385 PCTIiBOO/00923 Arg Ile His Giy ASn Vai 390 Gly Tyr Leu Ala Ala Leu Glu Giu Ile Asn Gin Ile Ser 395 Val Ser Giu Ser Ala Ala Pro Ala Val Ala Leu Giu Arg 410 Met Leu Ser Val Val Pro 415 Ala Ala Pro Thr Pro Ser Ser Ser 425 Lys Asp Ala <210> 607 <211> 954 <212> DNA <213> Corynebacteriun glutarnicum <220> <221> <222> <223>

CDS

(101)..(931) RXA01073 <400> 607 taaccgactc cagcactaaa ctccaaaccc ttggcccgca ccgccaaagt ttagcgcgcc ccaagacacc accgcgccat gtttgcctag gattaggtac atg aca aac act Met Thr Asn Thr 1 acc gag atc att Thr Gu Ile Ile gaa cta aag gtg Giu Leu Lys Val cca gca atc gac Pro Ala Ile Asp gtg gcc Val Ala aag gaa gtt Lys Giu Val tcc cat aca Ser His Thr ttc cgt gtg cag Phe Arg Val Gin t tc Phe ctc gtc gat tac Leu Val Asp Tyr ctg cgg gct Leu Arg Ala cag gat tcc Gin Asp Ser aaa ggc ttt gtt Lys Gly Phe Vai ggt att tca ggt Gly Ile Ser Gly act ctt Thr Leu gcg gga cga ctc Ala Gly Arg Leu cag ctg gca gta gag cgc att cgt gcg Gin Leu Ala Val Giu Arg Ile Arg Ala gaa Giu gaa aac agc acg Giu Asn Ser Thr tat gtc ttc tac Tyr Val Phe Tyr gtt cgc ctc ccc Val Arg Leu Pro gcg atc cag gca Ala Ile Gin Ala gag gac gat gcg Giu Asp Asp Ala caa Gin gtt gca ttg gaa Val Ala Leu Giu ttc atc Phe Ile 100 gca cct gac Ala Pro Asp acc gaa gca Thr Giu Ala 120 agc gtg acc gtc Ser Val Thr Val aac gtt aaa gac gca Asn Val Lys Asp Ala 110.

acg gac gcc Thr ASP Ala 115 ctg acc gac Leu Thr Asp act gtt gca gct Thr Val Ala Ala ttg gaa ctt cct Leu Giu Leu Pro gag Giu 130 WO 01/00843 WO 0100843PCTIiBOO/00923 ttc aat cgg ggc aat att aaa gct cgc caa cgc atg Phe Asn Arg Gly Asn Ile 135 gca Al a 150 gct Ala gac Asp Cctg Leu gct Ala ggt Gly 230 gtc Val1 cag atc gca ggc cag Ile Ala Gly Gin gaa aac gtc acg Glu Asn Val Thr 170 ctg ctt cct ttg Leu Leu Pro.Leu 185 gag cac ctg ggt Giu His Leu Gly 200 gat ttg gaa gag Asp Leu Giu Giu 215 gtg tcg tat gcg Val Ser Tyr Ala agt gaa aaa gcc Ser Giu Lys Ala 250 cac aag cgc cac Lys 140 ggC Gly ttC Phe ggt Gly Cct Pro cgc Arg 220 atc Ile cag Gin cct Ala Arg Gin Arg Met ttg Leu ttc Phe ttg Leu tca Ser 205 cca Pro gat Asp cgc Arg gct Ala att Ile 160 ttC Phe cgt Arg tgg Trp cca Pro ctg Leu 240 cac His cag Gin 145 ggc act gat Gly Thr Asp ggt gat ggc Gly Asp Gly caa gga gct Gin Giy Ala 195 acc aag gtt Thr Lys Val 210 gat gag gaa Asp Giu Giu 225 gaa aac aag Giu Asn Lys ctg tgg aag Leu Trp Lys gaa aat tgg Giu Asn TrD gtt gcC Val Ala cag Gin cac His gca Ala 180 gcc Ala cct Pro Gin His Lys Arg His Leu Pro 265 taatccaaca gtttgagtgt cgc <210> 608 <211> 277 <212> PRT <213> Corynebacteriun giutanicun <400> 608 Met Thr Asn Thr Gin Thr Giu Ile 1 5 Ala Ilie Asp Val Ala Lys Giu Val Asp Tyr Leu Arg Ala Ser His Thr 40 Gly Gly Gin Asp Ser Thr Leu Ala 55 Giu Arg Ile Arg Ala Giu Giu Asn 70 Leu Lys Val Ser Pro Val Gin Phe Leu Val Val Leu Gly Ile Ser Thr Gin Leu Ala Val Tyr Val Phe Tyr Ala WO 01/00843 PCTIiBOO/00923 a Gin Val Val1Arg Leu Pro Tyr Ala Ile Gin Ala Asp Giu Asp Asp Al 90 Al a Asp Pro Met 145 Giy Gly Gin Thr Asp 225 Glu Leu Glu Leu Al a Giu 130 Val1 Thr Asp Giy Lys 210 Giu Asn Trp Asn Giu Thr 115 Leu Ala Asp Gly Ala 195 Val Giu Lys Lys Trp 275 Phe 100 Asp Thr Gin Hils Al a 180 Ala Pro Ala Pro Val 260 Trp Ala Thr Phe Ala 150 Al a Asp Leu Al a Gly 230 Val1 Gin Asp Ala 120 Arg Ala Asn Leu His 200 Leu Ser Giu Lys

LYS

105 Thr G ly Gly Val Pro 185 Leu Giu Tyr Lys Arg 265 Ser Val1 Asn Gin Thr 170 Leu Gly Giu Ala Ala 250 His Thr Ala Lys 140 Gly Phe Gly Pro Arg 220 Ile Gin Pro Val Ala 125 Ala Leu Phe Leu Ser 205 Pro Asp Arg Ala Asn 110 Leu Arg Leu Thr Ser 190 Ser Ala Asn Ile Thr 270 Val1 Giu Gin Val1 Lys 175 Lys Thr Leu Tyr Giu 255 Pro

LYS

Leu Arg Ile 160 Phe Arg Trp Pro Leu 240 His Gin <210> 609 <211> 1461 <212> DNA <213> Corynebacteriui giutamicui <22 0> <221> CDS <222> (101)..(1438) <223> RXN02754 <400> 609 attatgaagc catcggagtt ggtgtggcct acaagggtga tcatgcgtgg atagtggtgg agttcactgt agctcccgct gattccgtag aatcaacaga gtg aat acc aat ccg Val Asn Thr Asn Pro 1 tct gaa ttc tcc tca aac cgt tca aca gct ctc ctt act gat aaa tat Ser Giu Phe Ser Ser Asn Arg Ser Thr Ala Leu Leu Thr Asp Lys Tyr 15 WO 01/00843 gag citg ac Glu Leu Tb ccc tca ac Pro Ser Tb 4 tac ggt gi: Tyr Gly Va ?ztt gta tt Phe Val Ph cgt acc ci: Arg Thr Le ggc tac cg Gly Tyr Ar gtg cgi: gg Val Arg Gi 12 tcc atc at Ser Ile Me 135 gtc acc gc Val Thr Al 150 acc cac ga Thr His Gi gga ttc tc Gly Phe Se cca gca tc Pro Ala Se 2C gat gac gg ASP ASP GI 215 tcc ctc gG Ser Leu GI 230 caa ggt gi: Gin Gly Va gca Ala ittt Phe aca Thr caa Gin cgc Arg atc Ile gaa Glu tct Ser 140 cgc Arg gtc Val aac Asn gcc Ala gaa Glu 220 acc Thr att Ile gca Ala gag Giu att Ile t ta Leu caa Gin ctt Leu 115 gtc Val1 gcg Ala tcc Ser tac Tyr tac Tyr 195 cac His cag Gin gac Asp ctt Leu PCT/BOOIOO923 gaa cgc 211 Glu Arg cgc cga 259 Arg Arg cgt gac 307 Arg Asp gac gac 355 Asp Asp gtt gat 403 Val Asp 100 ctg act 451 Leu Thr ati: ttg 499 Ile Leu cgc atg 547 Arg Met agg cgc 595 Arg Arg 165 ctc gct 643 Leu Ala 180 gga att 691 Gly Ile atc aac 739 Ile Asn gtt gaa 787 Val Glu atc acc 835 Ile Thr 245 ggt ggc 883 Gly Gly 260 gta cgt atc gac tcc ggc gac cta ggt gtg ctt gcc cga aag gtc cgc WO 01/00843 Val Arg Ile aag cag ctc Lys Gin Leu 280 PCT/IBOO/00923 Asp 265 gac Asp Ser Gly Asp Leu gat ctc aac Asp Leu Asn Giy Vai Leu Aia 270 cac aac acc aag His Asn Thr Lys gcg ggt ctt cgc Aia Giy Leu Arg 305 Arg Lys Val Arg 275 att gtg gtc tcc Ile Vai Vai Ser 290 ggc gaa cca gtt Giy Giu Pro Vai 979 tcc gac 1027 Ser Asp 295 gac gtc 1075 Asp Vai 310 acc gct 1123 Thr Aia gcc aag 1171 Aia Lys gtg cgc 1219 Vai Arg ctg gat gaa ttc Leu Asp Giu Phe ttt ggc gtt ggc Phe Giy Vai Gly gcc Ala 300 acc tcc gtt gtc aca Thr Ser Val Vai Thr 320 aag atc ggg gaa gtt Lys Ile Giy Glu Val 335 ggt tct ggc gca cca Giy Ser Giy Ala Pro 325 gcc ggt cac cct gtg Ala Giy His Pro Vai 340 ggc ctc gtg Gly Leu Vai 330 cgt tcc cga Arg Ser Arg aac Asn aag gaa agc Lys Giu Ser 345 acc cac Thr His 360 350 cgc aag tcc ggt acc Arg Lys Ser Giy Thr tac ggt ggt ggc aag aag gct Tyr Giy Giy Giy Lys Lys Ala 355 gca atc gaa gaa atc gtc tac Ala Ile Giu Glu Ile Val Tyr 370 gga aag ctc gac act ttg agc Gly Lys Leu Asp Thr Leu Ser cca ttc aat 1 267 Pro Phe Asn 375 ctg acc at~c 1315 Leu Thr Ilie 390 act ttg gaa 1363 Thr Leu Giu gcc gaa gca cca Ala Giu Aia Pro 380 cca ttg atg cgc Pro Leu Met Arg 395 act Thr gac Asp 385 ggt gaa atc gtt Giy Giu Ile Val cca ggt ttg cct Pro Gly Leu Pro 405 400 gat tcc Asp Ser 410 cca tgg 1411 Pro Trp, cgt ttc 1458 Arg Phe gaa ggc Giu Giy 425 gtg ggt Val Gly ctt Leu t tc cga gcg tat ttg Arg Ala Tyr Leu gca ctg tct cgc Ala Leu Ser Arg 430 ccg ccg gcc gct Pro Pro Ala Ala gcc aag caa ttg gtc tct tta Ala Lys Gin Leu Val Ser Leu 415 420 gat gag cct gtt ttg cac act Asp Glu Pro Val Leu His Thr 435 tagacaattc ggtctcacca 440 445 aac 1461 WO 01/00843 WO 0100843PCTAJBOOIOO923 <210> 610 <211> 446 <212> PRT <213> Corynebacteriun glutanicun <400> 610 Val Asn Thr Asn Pro Ser Glu Phe Ser 1 Leu Gly Pro Lys Asp Thr Ser Glu Ala 145 Met Ala Tyr Leu Lys 225 Thr Pro Ala Thr Ser Asn Ala Phe Gly Pro Thr 130 Ala Gly Ala Arg Leu 210 Ala Tyr Asp Arg Asp Ala Glu Ile Leu Gln Leu 115 Val1 Ala Ser Tyr Tyr 195 His Gin Asp Leu Lys 275 5 Tyr Arg Arg Asp Asp Asp Thr Leu Met Arg 165 Al a I le Asn Glu Thr 245 Gly Arg Ser Leu Glu Ala Glu Tyr Gly Phe Ala Asp Ser 170 Thr Thr Pro Asp Thr 250 Ser Asp Asn Gln Val Gly Giu 75 Leu Glu Ala Asp Gly 155 Ala Ser Ser Asn Thr 235 Ala Gly Leu Arg Ala Phe Thr Gin Arg I le Glu Ser 140 Arg Val Asn Ala Glu 220 Thr Ile Asp Asn Thr Leu Arg Arg Ala Tyr Phe 110 Val1 Val1 Ile Ala Glu 190 Ala Ala Leu Val1 Gly 270 His Leu Asp Leu Leu Leu Phe Gln Leu Ser Glu 160 Arg Ala Thr Phe Asp 240 Gly Leu Thr Lys Ile Val Val Ser Ser Asp Leu Asp Glu Phe Ala Ile Ala Gly Leu WO 01/00843 290 Arg Gly Giu 305 Giy Ser Gly Ala Gly His Gly Gly Lys 355 Giu Glu Ile 370 Leu Asp Thr 385 Val Pro Giy Gin Leu Vai Pro Val Leu 435 PCT/IBOO/00923 Pro Ala Pro 340 Lys Val Leu Leu Ser 420 His Phe Gly Arg Thr 360 Asn Ile Giu Giu Val1 440 Gly Leu Ser 345 His Ala Pro Asp Gly 425 Gly 300 Thr Lys Lys Ser Pro 380 Arg Ala Leu Pro Ser Ile Glu Gly 365 Asp Asp Tyr Ser Ala 445 Thr 320 Val Gly Ile Lys Ile 400 Lys Glu <210> 611 <211> 531 <212> DNA <213> Corynebacteriun giutamicun <220> <22i> CDS <222> (101)..(508) <223> RXA02299 <400> 611 acgcgggggt tgttgccgga tcgaaatatt cctttccttg taaaacttgc aggacaaccc ccataaggac accacaggac ctc Leu tat Tyr ttg Leu cgt Arg agt Ser ggC Gly gaa Giu gaa Glu cga Arg atc Ile gtt Val1 att Ile gcc act gtc act Ala Thr Val'Thr gac gcc gac ctg Asp Ala Asp Leu gcc atc gta gac Ala Ile Val Asp 45 gtg ggc gac gcc Val Gly Asp Ala 871 tcatctcacg ctatgatttc atg ctg cgc acc atc Met Leu Arg Thr Ile 1 caa gct gat cta gat Gin Ala Asp Leu Asp gtt cac gcc gcc gga Val His Ala Ala Giy atc acc aac ggc gct Ile Thr Asn Gly Ala gga acg ggc aat att Gly Thr Gly Asn Ile 115 163 211 259 307 WO 01/00843 tgc atc aa Cys Ilie As atc atc at Ile Ile Me PCT/1-BOO/00923 ggt gcc gct gca cac ctt a Gly Ala Ala Ala H-is Leu I 75 agc tac ctt caggca act g, Ser Tyr Leu Gin Ala Thr A ctt gtg Leu Val gcg tat Ala Tyr 100 gcg ctc Ala Leu acg tcg Thr Ser gag cca aag att gtg cac gtg gac Giu Pro Lys Ile Vai His Val Asp 105 ggc aac gat ctt gcg gaa gca ctac Giy Asn Asp Leu Ala Glu Ala Leu 120 125 aga agc att tagcgtttta gctcgccaal Arg Ser Ilie 135 <210> 612 <211> 136 <212> PRT <213> Corynebacteriun glutainicum <400> 612 Met Leu Arg Thr Ile Leu G~ly Ser I 1 Gin Ala Asp Leu Asp Tyr Val Gly! Vai His Ala Ala Gly Leu Ilie Glu 40 Ile Thr Asn Gly Ala Arg Leu Glu Gly Thr Gly Asn Ile Cys Ilie Asn( 70 Pro Gly Asp Leu Val Ilie Ilie Met! Glu Ala Lys Ala Tyr Glu Pro LysI 100 Arg Ile Val Ala Leu Gly Asn Asp 1 115 120 Giy Leu Leu Thr Ser Arg Ser Ile 130 135 <210> 613 <211> 960 <212> DNA <213> Corynebacteriun glutamicun ;cc g, kla A :ct g, Pro G tatt Ala Asp Ala Val His Ala Asp Leu 125 Thr Leu Asp Al a Asn Ala Asn Ser WO 01/00843 WO 0100843PCTI[BOO/00923 <220> <221> CDS <222> (101)..(937) <223> RXA01928 <400> 613 gcgagtacgc caccttgggc gattccttgc acgacgccgc tccacgcggg taccttccca ggcgaagcgg agtcctttta gcaggcctac atcgccgata atg cag gta gca acc ii! Met Gin Val Ala Thr aca aag cag gcg Thr Lys Gin Ala atc gac gcc ctc ctc cac cac aaa tcc Ile Asp Ala Leu Leu His His Lys Ser gtc ggg Val Gly ctc gtc ccc Leu Val Pro aaa gca gca Lys Ala Ala atg ggt gcg cta Met Gly Ala Leu agc gga cac gcc Ser Gly His Ala tcg ttg gtt Ser Leu Val att ttt gtc Ile Phe Val 211 259 cgc gct gaa aac Arg Ala Giu Asn act gtt gta gcc Thr Val Val Ala aat ccc ctg cag ttt gaa Asn Pro Leu Gin Phe Giu ctc ggt gat tgc Leu Gly Asp Cys gat tac cgc aac Asp Tyr Arg Asn ccc cgc caa ctc Pro Arg Gin Leu gac Asp gcc gat tta gca Ala Asp Leu Ala ctt gaa gag gca Leu Giu Giu Ala gtg gat att gtg Val Asp Ile Val ttc Phe gca ccc gat gtg Ala Pro Asp Val gaa atg tac ccc Giu Met Tyr Pro ggt ggc Gly Giy 100 ttg cca cta Leu Pro Leu ggt gcc agc Gly Ala Ser 120 tgg gcg cgc acc Trp Ala Arg Thr tcc atc gga aca Ser Ile Gly Thr aaa ttg gag Lys Leu Giu 115 gtg gtg gcg Val Val Ala 451 499 agg cct ggc cat Arg Pro Gly His gat ggt gtg gct Asp Gly Val Ala aag ctg ttc aat ttg gtg Lys Leu Phe Asn Leu Val cgc Arg 140 tct gat cgt gca Pro Asp Arg Ala ttt gga caa aaa Phe Gly Gin Lys gct cag cag gtt Ala Gin Gin Val gcg Ala 155 gtg att cgg cga Val Ile Arg Arg gtt gcc gat cta Val Ala Asp Leu att ccc gtg gag Ile Pro Vai Giu cgt ccc gtt ccg att att cgt ggc gcc Arg Pro Val Pro Ile Ile Arg Giy Ala 175 gat ggc Asp Gly 180 tta gcc gaa Leu Ala Giu agc cgc aat caa cgt ctt tct gcg gat Ser Arg Asn Gin Arg Leu Ser Ala Asp 190 cag cga gcg Gin Arg Ala 195 WO 01/00843 caa gct ctg gtg ctg ccg cag gtg ttg Gin Ala Leu Val Leu Pro Gin Vai Leu 200 205 gca gct ggt gaa gcg cta gat atc caa Ala Ala Giy Giu Ala Leu Asp Ile Gin 215 220 agc gcc gac ggc gtg cgc ttg gat cac Ser Ala Asp Gly Val Arg Leu Asp His 230 235 acc ctc gaa cca tta gaa atc gac ggc Thr Leu Giu Pro Leu Giu Ile Asp Gly 250 gtg gtc ggc gcg att ttc gtg ggg ccg Val Val Gly Ala Ile Phe Val Gly Pro 265 270 gag ctc tagtaccaac cctgcgttgc agc Giu Leu <210> 614 <211> 279 <212> PRT <213> Corynebacterium giutamicun <400> 614 Met Gin Val Ala Thr Thr Lys Gin Ala PCT/iBOO/00923 ira aaa 739 ggg ttg Gly Leu gcg cgc Ala Arg 225 gaa att Giu Ile 240 ctc acc Leu Thr cgg ttg Arg Leu Val1 Leu Phe Arg Ala Gly 100 Leu Val Gin G iy Val1 Val Asn Gly Giy Giu Ala Lys Leu Val Pro Thr Lys Ala Ala Arg Asn Pro Leu Gin Tyr Pro Arg Gin Val Asp Ile Val Leu Pro Leu Val 105 Gly Ala Ser Arg 120 Lys Leu Phe Asn 135 Asp Ala Gin Gin 150 Leu Met Al a Phe Leu Phe Trp Pro Leu Val Ile Gly Giu Giu Asp Ala Al a Gly Val1 Al a 155 Asp Ala Asn Ala Ala Pro Arg His Arg 140 Val1 WO 01/00843 WO 010084PCT[IBOO/00923 Val1 Arg Ala Leu Arg 225 Ile Thr.

Leu Leu Asp 180 Arg Arg Leu Pro Ala 260 Asn Asp 165 Gly Ala Lys Al a Ala 245 Leu Ile Val1 Giu Leu 200 Gly Asp Giu Gly Giu Ser 185 Val1 Giu Gly Pro Ala 265 Val1 Gin Val 205 Ile Asp Asp Gly Pro Arg 190 Leu Gin His Giy Pro 270 <210> 615 <211> 936 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(913) <223> RXN01929 <400> 615 aaaatttgac tccataacga gaacttaatc gagcaacacc ggaatttatt tattctgagc tggtcatcac atctatactc att gat Ile Asp aac ggc Asn Gly cgc att Arg Ile gct gcc Ala Ala gat gag Asp Giu aaa arc Lys Ile gtt tcg Val Ser gag gct Giu Ala gtg ctg Val Leu gtg ctg Val Leu 75 cgc Arg gtt Val1 ggC Gly gg t Gly 60 gcc Ala cgt Arg acc Thr gat Asp gat Asp gcg Ala cctgaacagt gaatcaaatc atg ccc atg tca ggc Met Pro Met Ser Giy 1 cgc gaa gct aaa gra Arg Giu Ala Lys Val gat gcg ctt tcg gcg Asp Ala Leu Ser Ala ctt gtt ggt gat tcc Leu Val Gly Asp Ser ttg tcg atc acc ttg Leu Ser Ile Thr Leu atc gct acg aag cgt le.Ala Thr Lys Arg 115 163 211 259 307 355 WO 01/00843 WO 0100843PCT/EBOO/00923 gcg ctt gtg gtg Ala Leu Val Val gtt Val gat ctg ccg ttt Asp Leu Pro Phe acc tat gag gtg Thr Tyr Giu Val agc cca Ser Pro 100 403 451 aat cag gcg Asn Gin Ala gct gcg gtg Ala Ala Val 120 gag tcc gcg atc Giu Ser Ala Ile Cgg Arg 110 gtc atg cgt gaa acg ggt gcg Val Met Arg Giu Thr Gly Ala 115 aag atc gag ggt Lys Ile Glu Gly gtg gag atc gcg Val Giu Ile Ala cag Gin 130 acg att cga Thr Ile Arg cgc att Arg Ile 135 gtt gat gct gga Val Asp Ala Gly ccg gtt gtC ggc Pro Val Val Giy cac His 145 atc ggg tac acc Ile Gly Tyr Thr cag tcc gag cat Gin Ser Giu His ttg ggc ggc cac Leu Giy Gly H-is gtt cag ggt cgt Val Gin Gly Arg ggc Gly 165 gcg agt tct gga Ala Ser Ser Gly ctc atc gcc gat Leu Ile Ala Asp cgc gcg ttg gag Arg Ala Leu Glu cag gcg Gin Ala 180 ggt gcg ttt Gly Ala Phe gag gtt acc Giu Vai-Thr 200 gtt gtg ttg gag atg gtt cca gca gag Val Val Leu Giu Met Val Pro Ala Giu 190 gca gcg cgc Ala Ala Arg 195 ggt gcc ggc Gly Ala Gly gag gat ctt tcc Glu Asp Leu Ser atc Ile 2.05 acc act atc gga Thr TIhr Ilie Gly aat ggc Asn Gly 215 aca gat ggg cag Thr Asp Gly Gin ttg gtg tgg cag gat gcc ttc ggC ctc Leu Val Trp Gin Asp Ala Phe Gly Leu 225 aac Asn 230 cgc ggc aag aag Arg Gly Lys Lys cgc ttc gtc cgC Arg Phe Val Arg tac gcc acc ttg Ty~r Ala Thr Leu ggC Gly 245 gat tcc ttg cac gac gcc gcg cag gcc tac Asp Ser Leu His Asp Ala Ala Gin Ala Tyr 250 255 atc gcc gat atc Ile Ala Asp Ile cac gcg His Ala 260 ggt acc ttc Gly Thr Phe ggc gaa gcg gag Gly Giu Ala Giu tcc ttt Ser Phe 270 taatgcaggt agcaaccaca aag <210> 616 <211> 271 <212> PRT <213> Corynebacterium giutanicun <400> 616 Met Pro Met Ser Gly Ile Asp Ala Lys 1 5 Lys Ile Arg Thr Arg His Phe 10 WO 01/00843 WO 0100843PCTJLBOOIOO923 Arg Asp Leu Leu Ile Tyr Arg Ala His 145 Val Ala Ala Gly Asp 225 Tyr Al a Lys Val Asn Gly Gin Lys 25 Val Ser Val Leu Thr Ser Tyr Ser Asp Thr Lys Ser 100 Gly Ile Tyr Arg Gin 180 Ala Ala Gly Leu His 260 Phe Asn Met Val1 Ala Val 120 Val1 Ser Ser Phe Thr 200 Thr Gly Leu Phe Asp Val1 Ile Val Val 105 Lys Asp Glu Gly Ala 185 Glu Asp Lys His Pro 265 Gly Gly Ala Leu Ala Giy Ile 140 Leu Ile Leu Ser Val1 220 Arg Ala Ala Met Thr Val1 Gly Val Giu Val His Ala 175 Val1 Thr Trp Arg Tyr 255 Phe Leu Thr Thr Thr Met Ile Gly Val 160 Arg Pro Ile Gin Glu 240 Ile <210> 617 <211> 930 <212> DNA <213> Corynebacterium giutanicun <220> <221> CDS <222> (101)..(907) <223> FRXA01929 <400> 617 tgactccata acgagaactt aatcgagcaa cacccctgaa cagtgaatca aatcggaatt 877 WO 01/00843 WO 0100843PCT/EB00/00923 tatttattct gagctggtca tcacatctat actcatgccc atg tca ggc att Met Ser Gly Ile 1 gca aag aaa atc Ala Lys Lys Ile acc cgt cat ttc Thr Arg His Phe gaa gct aaa gta Giu Ala Lys Vai aac ggc Asn Gly cag aaa gtt Gin Lys Vai gtt ctc acc agc tat gat gcg ctt tcg Vai Leu Thr Ser Tyr Asp Ala Leu Ser gcg cgc att Aia Arg Ile tcc gct gcc Ser Ala Ala ttt gat gag gct ggc gtc gat Phe Asp Glu Ala Gly Vai Asp atg Met ctc ctt gtt ggt Leu Leu Vai Giy aac gtt gtg ctg ggt cgc Asn Val Val Leu Giy Arg gat Asp acc acc ttg tcg Thr Thr Leu Ser acc ttg gat gag Thr Leu Asp Giu atg Met att gtg ctg gcc Ile Val Leu Ala gcg gtg acg atc gct acg aag cgt gcg Ala Val Thr Ile Ala Thr Lys Arg Ala gtg gtg gtt gat Val Val Vai Asp ccg ttt ggt acc Pro Phe Giy Thr gag gtg agc cca Glu Val Ser Pro aat cag Asn Gin 100 gcg gtg gag Ala Vai Giu gtg aag atc Val Lys Ilie 120 gcg atc cgg gtc Ala Ile Arg Val atg Met 110 cgt gaa acg ggt Arg Giu Thr Gly gcg gct gcg Ala Ala Ala 115 cga cgc att Arg Arg 11e gag ggt ggc gtg Glu Gly Giy Val gag Giu 125 atc gcg cag acg Ile Ala Gin Thr gtt gat Val Asp 135 gct gga att ccg Ala Gly Ilie Pro gtt Val1 140 gtc ggc cac atc ggg tac acc ccg cag Val Gly His Ile Gly Tyr Thr Pro Gin 145 tcc Ser 150 gag cat tcc ttg Giu His Ser Leu ggc cac gtg gtt Gly His Val Val cag Gin 160 ggt cgt ggc gcg Gly Arg Gly Ala tct gga aag ctc atc gcc gat gcc cgc Ser Gly Lys Leu Ile Ala Asp Ala Arg gcg Ala 175 ttg gag cag gcg Leu Giu Gin Ala ggt gcg Gly Ala 180 ttt gcg gtt Phe Ala Val acc gag gat Thr Giu Asp 200 gtg Val1 185 ttg gag atg gtt cca gca gag gca gcg Leu Giu Met Val Pro Ala Giu Ala Ala 190 cgc gag gtt Arg Giu Val 195 ggc aat ggc Gly Asn Gly ctt tcc atc acc Leu Ser Ilie Thr act Thr 205 atc gga atc ggt Ilie Gly Ile Gly aca gat ggg cag gtt ttg Thr Asp Gly Gin Val Leu 215 tgg cag gat gcc Trp Gin Asp Ala ttc Phe 225 ggc ctc aac cgc Gly Leu Asn Arg WO 01/00843 ggc aag aag Gly Lys Lys 230 ttg cac gac Leu His Asp ttc cca ggc Phe Pro Gly PCTIIBOO/00923 cca cgc Pro Arg gcc gcg Ala Ala 250 gaa gcg Glu Ala 265 ttc Phe 235 cag Gin gag Giu cgc gag tac gcc acc ttg ggc gat tcc Arg Glu Tyr Ala Thr Leu Gly Asp Ser 240 245 tac atc gcc gat atc cac gcg ggt acc Tyr Ile Ala Asp Ile His Ala Gly Thr 255 260 ttt taatgcaggt agcaaccaca aag Phe <210> 618 <211> 269 <212> PRT <213> Corynebacterium giutarnicum <400> 618 Met Ser Gly Ile Asp Ala Lys Lys Ile 1 Ala Leu Gly Ile Thr Val1 Thr Thr Gly 145 Gly Giu Ala Gly Lys Ser Asp Thr Lys Ser Gly Ile 130 Tyr Arg Gin Ala Ala 210 Val Al a Ser Leu Arg Pro Ala 115 Arg Thr Gly Ala Arg 195 Gly Lys Asp Val1 55 Ile Val Val Lys Asp 135 Giu Gly Ala Giu Asp 215 Val1 Glu Val Val1 Val Glu Ile 120 Ala His Lys Val Asp 200 Gly Ser Ala Leu Leu Asp Ser 105 Glu Gly Ser Leu Val1 185 Leu Gin Arg 10 Val Gly Gly Ala Leu Ala Gly Ile Leu Ile 170 Leu Ser Thr Leu Val Arg Lys 75 Pro Ile Gly Pro G ly 155 Ala Giu Ile Arg Thr Asp Asp Al a Phe Arg Val Val1 140 Gly Asp Met Thr Phe Tyr Leu Thr Thr Thr Met 110 Ile Gly Val1 Arg Pro 190 Ile Arg Asp Leu Leu Ile Tyr Arg Ala His Val1 Ala 175 Ala Giy Giu Ala Val Ser Ala Giu Giu Gin Ile Gin 160 Leu Giu Ile Vai Leu Val Trp Gin Asp Ala 220 WO 01/00843 WO 0100843PCTAiB0O/00923 Phe Gly Leu Asn Arg Gly Lys Lys Pro Arg Phe 225 230 235 Thr Leu Gly Asp Ser Leu His Asp Ala Ala Gin 245 250 Ilie His Ala Gly Thr Phe Pro Gly Giu Ala Giu 260 265 Val Arg Giu Tyr Ala Tyr Ile Ala Asp 255 Ser Phe <210> <211> <212> <213> <220> <221> <222> <223> 619 921

DNA

Corynebacteriun giutani curn

CDS

(101)..(898) RXA0 1521 <400> 619 accccggcag gcaacgcctt ttccgggatt tggcgcgcag gcaggcagag atttcccgcg cgcaaga tat tgagctgtgg gcaattcaga aggaggaccg ttg agt ttc acg Leu Ser Phe Thr ggt cag ggc aga Gly Gin Gly Arg ttt gat acc gtc gag cag atc cgc atg Phe Asp Thr Val Giu Gin Ile Arg Met ttc ggc Phe Gly agc gcc ctg Ser Ala Leu aat ggc ctc Asn Gly Leu aaa acc ggc aaa Lys Thr Gly Lys gtg gtg ctc gta Val Val Leu Val ccc ttg gga Pro Leu Gly gca aaa cgc Ala Lys Arg cac gca ggc cat His Ala Gly His gcg ctc atc cgc Ala Leu Ile Arg atc ccc Ilie Pro ggt gcg gtg gtc Gly Ala Val Val gtc gcc tat gcc Val Ala Tyr Ala ggc Gly ccg gaa tcg gat Pro Glu Ser Asp cac His gca cgt tta agg Ala Arg Leu Arg gaa Giu gag ctt atc gac Giu Leu Ile Asp atc ttc ccg ttc Ile Phe Pro Phe ccc gaa acg cta Pro Glu Thr Leu cct cac ggc atc Pro His Gly Ile gtg gaa gtt aca Val Glu Val Thr ggt ggc Gly Gly 100 cca aca ctt Pro Thr Leu ttg gga atc Leu Gly Ile 120 cca caa ggt gcg Pro Gin Gly Ala gaa Giu 110 gta acc aag gtg Val Thr Lys Val ctg ggg ctg Leu Gly Leu 115 aag gac tat Lys Asp Tyr acc gga gca act Thr Gly Ala Thr gat Asp 125 gtg gtg ctc ggt Val Val Leu Gly gag ctg gtg gtt cta gtc cag cgc gcc ctt aat gat ctg cat att cca Giu Leu Val Val Leu Val Gin Arg Ala Leu Asn Asp Leu His Ilie Pro WO 01/00843 WO 0100843PCT/IBOO/00923 135 140 145 gta aaa ctg cat tct gtt cca acc gtg cgc atg cca gat gga cta gcc 595 Val Lys Leu His Ser Val Pro Thr Val Arg Met Pro Asp Gly Leu Ala 150 155 160 165 att tcc ctg cgt aat att tca gtg ccc gaa gac tcc cgc gaa acg gca 643 Ilie Ser Leu Arg Asn Ile Ser Val Pro Giu Asp Ser Arg Glu Thr Ala 170 175 180 ttg agc ctg gca gca gcc ctc acc gcc ggt gcg cat tcg gca gaa cac 691 Leu Ser Leu Ala Ala Ala Leu Thr Ala Gly Ala His Ser Ala Giu His 185 190 195 ggc gag gca gtg gtt aaa gaa aca gtc acg caa gtg ctc aaa gcc gca 739 Gly Glu Ala Val Val Lys Glu Thr Val Thr Gin Val Leu Lys Ala Ala 200 205 210 ggc gtg acc ccc gat tat gta gaa atc cgt ggc ctg gat ctt gga cca 787 Gly Val Thr Pro Asp Tyr Val Glu Ilie Arg Gly Leu Asp Leu Gly Pro 215 220 225 gcc ccc gaa atc gga gac gcc cga ctc ttc gca gcc atc acg ctt ggc 835 Ala Pro Glu Ile Gly Asp Ala Arg Leu Phe Ala Ala Ile Thr Leu Gly 230 235 240 245 gat gtc caa ctc cac gac aac gtc ggc cta ccc ctt gga atc ggc ttc 883 Asp Val Gin Leu His Asp Asn Val Gly Leu Pro Leu Gly Ile Gly Phe 250 255 260 aaa aac atc gaa ggc tgatcccggt ttacccagtt cgc 921 Lys Asn Ilie Glu Gly 265 <210> 620 <211> 266 <212> PRT <213> Corynebacteriun glutamicum <400> 620 Leu Ser Phe Thr His Gly Gin Gly Arg Val Phe Asp Thr Val Giu Gin 1 5 10 Ile Arg Met Phe Gly Ser Ala Leu Arg Lye Thr Gly Lys Pro Val Val 25 Leu Val Pro Leu Gly Asn Gly Leu His Ala Gly His Ile Ala Leu Ile 40 Arg Ala Ala Lye Arg Ile Pro Gly Ala Val Val Val Val Ala Tyr Ala 55 Gly Pro Glu Ser Asp His Ala Arg Leu Arg Glu Glu Leu Ile Asp Ala 70 75 Ile Phe Pro Phe Asn Pro Glu Thr Leu Trp, Pro His Gly Ile Arg Val 90 Glu Val Thr Gly Gly Pro Thr Leu Thr Pro Gin Gly Ala Giu Val Thr 100 105 110 881 WO 01/00843 WO 0100843PCTIBOO/00923 Lys Gly Asp 145 Pro Ser His Val1 Leu 225 Ala Leu Leu Glu Val1 150 Ile Leu G ly Gly Ala 230 Asp Lys Gly Leu 135 Lys Ser Ser Giu Val1 215 Pro Val1 Asn Val1 Ala Val1 Pro Ala 190 Vai Ile Leu Gly Val Leu Arg Glu 175 Gly Thr Arg Phe Leu 255 <210> 621 <211> 1137 <212> DNA <213> Corynebacterium giutamicui <220> <221> CDS <222> (1114) <223> RXS01145 <400> 621 taatgtagtt gtctgcccaa gcgagttaaa ctcccacgat cttttcacca aaatttttac gaaaggcgag attttctccc ctt tat gat gct gac gct gac ctc tcc ttg atc Leu Tyr Asp Ala Asp Ala Asp Leu Ser Leu Ile 15 gcc atc gtt ggc tac ggc tcc cag ggc cac gca Ala Ile Val Gly Tyr Gly Ser Gin Gly His Ala 30 cgc gat tct ggc gtt gag gtt gtc att ggt ctg Arg Asp Ser Gly Val Glu Val Val Ile Gly Leu 45 ttacagtggg gggcagacat atg gct att gaa ctg 115 Met Ala Ile Glu Leu 1 cag ggc cgt aag gtt 163 Gin Gly Arg Lys Val cac tcc cag aac ctc 211 His Ser Gin Asn Leu cgc gag ggc tcc aag 259 Arg Glu Gly Ser Lys WO 01/00843 WO 0100843PCTIBOOIOO923 tcc gca Ser Ala gag aag gca aag Giu Lys Ala Lys gca ggc ttc gag Ala Gly Phe Glu aag acc acc gct Lys Thr Thr Ala gct gca gct tgg Ala Ala Ala Trp gac gtc atc atg Asp Vai Ile Met ctg gct cca gac Leu Ala Pro Asp tcc cag gca gaa Ser Gin Ala Giu ttc acc aac gac Phe Thr Asn Asp gag cca aac ctg Glu Pro Asn Leu aac gca Asn Ala 100 ggc gac gca Gly Asp Ala atc aag cca Ile Lys Pro 120 ctg Leu 105 ctg ttc ggc cac Leu Phe Gly His ggC Gly 110 ctg aac att cac ttc gac ctg Leu Asn Ile His Phe Asp Leu 115 gct gac gac atc Ala Asp Asp Ilie gtt ggc atg gtt Val Gly Met Vai gcg Ala 130 cca aag ggc Pro Lys Gly cca ggc Pro Gly 135 cac ttg gtt cgc His Leu Val Arg cag ttc gtt gat Gin Phe Val Asp ggc Giy 145 aag ggt gtt cct Lys Giy Val Pro tgc ctc atc gca gtc gac cag gac cca acc Cys Leu Ile Ala Val Asp Gin Asp Pro Thr 150 155 acc ctg tcc tac gca gca gca atc ggt ggc Thr Leu Ser Tyr Aia Ala Ala Ile Giy Gly 170 175 gga Gly 160 acc gca cag gct Thr Ala Gin Ala c tg Leu 165 gca cgc gca ggc Ala Arg Ala Giy gtt atc Val Ile 180 cca acc acc Pro Thr Thr gct gtt ctc Ala Val Leu 200 t tc Phe 185 gaa gct gag acc Glu Ala Giu Thr acc gac ctc ttc Thr Asp Leu Phe ggc gag cag Gly Giu Gln 195 ggc ttc gag Gly Phe Giu tgc ggt ggc acc Cys Giy Gly Thr gaa ctg gtc aag Glu Leu Vai Lys gt t Val 210 gtt ctc Val Leu 215 acc gaa gct ggc Thr Giu Ala Gly gag cca gag atg Glu Pro Giu met tac ttc gag gtt Tyr Phe Glu Val ctt cac gag ctc aag Leu His Glu Leu Lys 230 agc aac atg aac tac Ser Asn Met Asn Tyr 250 ctc Leu 235 atc gtt gac ctc Ile Val Asp Leu ttc gaa ggt ggc Phe Giu Giy Gly tct gtt tct gac Ser Val Ser Asp gct gag ttc ggt Ala Glu Phe Gly ggc tac Gly Tyr 260 ctc tcc ggc Leu Ser Gly gac atc ctg Asp Ile Leu 280 cca Pro 265 cgc gtc atc gat Arg Val Ile Asp gac acc aag tcc Asp Thr Lys Ser cgc atg aag Arg Met Lys 275 cgc ctc atc Arg Leu Ile acc gat atc cag Thr Asp Ile Gin ggc acc ttc acc Gly Thr Phe Thr aag Lys 290 WO 01/00843 gca aac gtt 1027 Ala Asn Val 295 tac aac aac 1075 Tyr Asn Asn 310 atg agc tgg 1124 Met Ser Trp PCT/IBOO/00923 gag aac Glu Asn cac cca His Pro gtc aag Val Lys 330 ggc aac acc gag Gly Asn Thr Glu 300 atc gag gag acc Ile Glu Giu Thr 315 gtt gac gct cgc Val Asp Ala Arg gag ggc ctt cgt gct tcc Glu Gly Leu Arg Ala Ser 305 gct aag ctc cgc gac ctc Ala Lys Leu Arg Asp Leu 320 325 gaa acc gct taagtttcac Glu Thr Ala ccctttgacg gct 1137 <210> 622 <211> 338 <212> PRT <213> Corynebacteriun glutanicun <400> 622 Met Ala Ile Glu Leu Leu Tyr Asp Ala Arg Gin Gly Thr Pro Leu Phe 115 Pro Gly Gin Gly Lys Asn Ser Thr

ASP

Asn 100 Asp Lys Val1 Al a Val1 180 Val Leu Lys Ala Thr Ala Leu Gly Pro Leu 165 Ile Ala Arg Ser Glu Ser Gly Ile Pro Cys 150 Thr Pro Gly Gly Lys Ala Glu Leu 105 Ala Leu Ala Tyr Phe 185 Asp Ser Val1 Glu Asp Thr Gly Ile Arg 140 Gin Ala Glu Leu Gin Val Ala Val Asn His Ile 125 Gin Asp Ile Thr WO 01/00843 WO 0100843PCTA~BOO/00923 Leu Lys Ala 225 Phe Glu Lys Thr Gly 305 Lys Thr Gly 195 Gly Phe Gly Gly Arg 275 Arg Arg Arg Glu Phe Giu Gly Gly 260 Met Leu Ala Asp Gly Gly Leu 235 Ser Val1 Ile Gly Ile 315 Val Thr Tyr 220 Ile Val1 Ile Gin Asn 300 Giu Asp <210> 623 <211> 556 <212> DNA <213> Corynebacterium glutainicum <220> <221> CDS <222> (101)..(556) <223> FRXA01145 <400> 623 taatgtagtt gtctgcccaa gcgagttaaa ctcccacgat cttttcacca aaatttttac gaaaggcgag attttctccc ctt tat gat gct gac gct gac ctc tcc ttg atc Leu Tyr Asp Ala Asp Ala Asp Leu Ser Leu Ile 15 gcc atc gtt ggc tac ggc tcc cag ggc cac gca Ala Ile Val Gly Tyr Gly Ser Gin Gly His Ala 30 cgc gat tct ggc gtt gag gtt gtc att ggt ctg Arg Asp Ser Gly Val Giu Val Vai Ile Gly Leu 45 tcc gca gag aag gca aag gaa gca ggc ttc gag 885 ttacagtggg gggcagacat atg gct att gaa ctg 115 Met Ala Ile Glu Leu 1 cag ggc cgt aag gtt 163 Gin Gly Arg Lys Val cac tcc cag aac ctc 211 His Ser Gin Asn Leu cgc gag ggc tcc aag 259 Arg Glu Gly Ser Lys gtc aag acc acc.gct 307 WO 01/00843 WO 0100843PCT/EBOO/00923 Ser Ala Glu Lys Ala Lys Glu Ala Gly Phe Glu Val Lys Thr Thr Ala 60 gag gct gca gct tgg gct gac gtc atc atg ctc ctg gct cca gac acc 355 Giu Ala Ala Ala Trp Ala Asp Val Ile Met Leu Leu Ala Pro Asp Thr 75 80 tcc cag gca gaa atc ttc acc aac gac atc gag cca aac ctg aac gca 403 Ser Gln Ala Glu Ile Phe Thr Asn Asp Ile Glu Pro Asn Leu Asn Ala 95 100 ggc gac gca ctg ctg ttc ggc cac ggc ctg aac att cac ttc gac ctg 451 Gly Asp Ala Leu Leu Phe Gly His Gly Leu Asn Ile His Phe Asp Leu 105 110 115 atc aag cca gct gac gac atc atc gtt ggc atg gtt gcg cca aag ggc 499 Ile Lys Pro Ala Asp Asp Ilie Ilie Val Gly Met Val Ala Pro Lys Gly 120 125 130 cca ggc cac ttg gtt cgc cgt cag ttc gtt gat ggc aag ggt gtt cct 547 Pro Gly His Leu Val Arg Arg Gln Phe Val Asp Gly Lys Gly Val Pro 135 140 145 tgc ctc atc 556 Cys Leu Ile 150 <210> 624 <211> 152 <212> PRT <213> Corynebacteriun glutamicum <400> 624 Met Ala Ile Giu Leu Leu Tyr Asp Ala Asp Ala Asp Leu Ser Leu Ile 1 5 10 Gin Gly Arg Lys Val Ala Ilie Val Gly Tyr Gly Ser Gin Gly His Ala 25 His Ser Gin Asn Leu Arg Asp Ser Gly Val Giu Val Val Ile Gly Leu 40 Arg Giu Giy Ser Lys Ser Ala Glu Lys Ala Lys Giu Ala Gly Phe Giu 55 Val Lys Thr Thr Ala Giu Ala Ala Ala Trp Ala Asp Val Ile Met Leu 70 75 Leu Ala Pro Asp Thr Ser Gin Ala Glu Ile Phe Thr Asn Asp Ile Giu 90 Pro Asn Leu Asn Ala Gly Asp Ala Leu Leu Phe Gly His Gly Leu Asn 100 105 110 Ilie His Phe Asp Leu Ile Lys Pro Ala Asp Asp Ile Ile Val Gly Met 120 125 Val Ala Pro Lys Gly Pro Giy His Leu Val Arg Arg Gin Phe Val Asp 130 135 140 WO 01/00843 WO 0100843PCT/IBOO/00923 Gly Lys Gly Val Pro Cys Leu Ile 145 150 <210> <211> <212> <213> <220> <221> <222> <223> 625 1389

DNA

Corynebacterium giutamicun

CDS

(101) (1366) RxA02239 <400> 625 ctatgcacag cggaggtcga gggagagcgt ggggtggcgt cgaaaagcat cttgtagagt gtgtgggaac ccagccagcc tcttactttg aaggattgtt gtg ccc atg act Val Pro Met Thr 1 gtt tca agc ccc Val Ser Ser Pro tcc Ser gca ccc cgc aac Ala Pro Arg Asn gtt gtt ggt gtt Val Val Gly Val gcc ggc Ala Gly gga atc gca Gly Ile Ala gcg ggc gat Ala Gly Asp tac aag gcg tgt Tyr Lys Ala Cys atc gtg cgc gcg Ile Val Arg Ala ttt aaa gaa Phe Lys Giu ttg aag ttt Leu Lys Phe aat gtg cgg gtg gtt cct acg gaa tcc Asn Val Arg Val Val Pro Thr Glu Ser gcg Ala gtg ggg Val Gly aag gcg acg ttt Lys Ala Thr Phe gcg ttg tct ggc Ala Leu Ser Gly ccg gtg tct aca Pro Val Ser Thr acg Thr gtg ttt gat gcg Val Phe Asp Ala gtg Val1 gat tcg gtg cag Asp Ser Val Gin gtg aaa gtt ggc Val Lys Val Gly gaa gct gat ttg Glu Ala Asp Leu gtg att gcg ccg Val Ile Ala Pro aca gcc gat ttg Thr Ala Asp Leu atg gcg Met Ala 100 cgt gtg gtg Arg Val Val gtg gca acg Val Ala Thr 120 ggt ctc ggt gac Gly Leu Gly Asp ga t Asp 110 ctg ttg gcg gcg Leu Leu Ala Ala acg ctg ctg Thr Leu Leu 115 tgc ccc gtg gtt Cys Pro Val Val att Ile 125 gcg ccg gcc atg cat acg gag atg Ala Pro Ala Met His Thr Giu Met 130 tgg ttt Trp Phe 135 aat ccg gct acc Asn Pro Ala Thr gcc aat gtg gca Ala Asn Val Ala ctg agg cag cgg Leu Arg Gin Arg 499 547 595 ggg Gly 150 att acc gtg att Ile Thr Val Ile cct gcg cat ggt Pro Ala His Gly ctc acc ggt aaa Leu Thr Gly Lys gat Asp 165 WO 01/00843 aca ggc cct ggc cgg ctg ccg gat cca gag cag att gtt gat Thr Gly Pro Gly Arg Leu Pro Asp Pro Glu Gin Ile Val Asp 170 175 aat gcg Asn Ala aaa gtg Lys Val cgc ttt Arg Phe 215 gaa atc Glu Ilie 230 gct gcg Ala Aia tcc aca Ser Thr gat ttc Asp Phe cag gcg 1027 Gin Ala 295 ttg agc 1075 Leu Ser 310 gtg aag 1123 Val Lys ggt ttt 1171 Giy Phe gcg cgc 1219 Ala Arg gc c Al a act Thr aat Asn cag Gin ccc Pro 250 atg Met atg Met aag Lys t cg Ser gaa Glu 330 gaa Giu ctg Leu ggg Gly gct Ala agt Ser cga Arg 235 act Thr ttt Phe gcg Ala ttg Leu ttg Leu 315 gca Ala act Thr cag Gin gcg Ala ggt Gly tcC Ser 220 ggt Gly ccg Pro gat Asp gca Ala aag Lys 300 gtg Val gga Giy gga Gly aag Lys gtg t tg Leu 190 acg Thr ggC Gly cat His ggc Giy gtc Val 270 gta Val ggc Gly aac Asn ctg Leu gag Giu 350 ggc Gly ggg Gly cag gat Gin Asp gag cat Giu His caa ggt Gin Gly 225 agc atc Ser Ile 240 gag atc Giu Ile gaa cga Giu Arg gat ttc Asp Phe gat tct Asp Ser 305 gat att Asp Ile 320 agt aat Ser Asn acc acc Thr Thr gac ctc Asp Leu aag cac gcg Ala 195 gat Asp gcg Ala gcg Ala ccg Pro ggc Gly 275 ccc Pro gaa Glu gc t Ala gtc Vali ttg Leu 355 atg Met gag PCT/TBOO/00923 tta gcc 643 Leu Aia 180 ggc aag 691 Gly Lys cct gtg 739 Pro Val ttg ggt 787 Leu Gly gga aat 835 Gly Asn 245 gtg gtg 883 Val Val 260 caa tct 931 Gin Ser gca tcg 979 Aia Ser gac gca Asp Ala acc acg Thr Thr 325 atc gtg Ile Val 340 gag tat Giu Tyr tgt aat Cys Asn ggc tgg gag gtg ggc atg ggc aaa 1267 Giu Vai Gly Met Gly Lys Val Phe Gin Lys His Asn Glu Gly Trp 385 WO 01/00843 WO 0100843PCT/IBOO/00923 att ttg gat gct cac ggt ggg gta gtc gat gtg gag cac ggc agc aaa 1315 Ile Leu Asp Ala His Gly Gly Val Val ASP Val Glu His Gly Ser Lys 390 395 400 405 atc gag gtt gct gcg caa att tgg gac gcg gca ctg gcg tat cgc gaa 1363 Ile Glu Val Ala Ala Gin Ile Trp Asp Ala Ala Leu Ala Tyr Arg Glu 410 415 420 gtc tagaaaaatc cagctagacc act 1389 Val <210> 626 <211> 422 <212> PRT <213> Corynebacteriun glutanicun <400> 626 Val Pro Met Thr His Val Ser Ser Pro 1 Val Gly Val Ala Arg Ala Phe Lys Ser Ala Leu Lys Asn Pro Val Ser Val Lys Val Gly Ala Asp Leu Met 100 Ala Ala Thr Leu 115 Met His Thr Glu 130 Thr Leu Arg Gin 145 Leu Thr Gly Lys Ile Val Asp Leu 180 Gly Ala Val1 Thr 70 Glu Arg Val1 Trp Gly 150 Thr Asn Ile Gly G ly Val Ala Val1 Ala Phe 135 Ilie G ly Ala Al a Asp 40 Lys Phe Asp Val1 Thr 1-20 As n Thr Pro Val Ala 25 Asn Ala Asp Leu Ala 105 Cys Pro Val Gly His 185 Ser 10 Tyr Val Thr Ala Ile Gly Pro Al a Ile Arg 170 Ala Thr Ala Lys Arg Phe Val 75 Val Leu Val Thr Glu 155 Leu Gly Ala Arg Cys Val Ala Ser.

Ala Asp Ile 125 Ala Ala Asp Arg Gly 205 Asn His Pro Leu Val1 Pro Asp 110 Ala Asn His Pro Leu 190 Thr Val Ile Thr Ser Gin Ala Leu Pro Val G ly Glu 175 Pro His Val1 Val Glu Gly His Thr Leu Ala Ala Arg 160 Gin Gin Glu Asp Leu Ala Gly Lys Lys Vai Leu Ile WO 01/00843 WO 0100843PCTIiBOO/00923 His Ile Asp Pro Val Arg Phe Ile Gly Asn Ser Ser Ser Gly Arg Gin 210 215 220 Gly Phe Ala Leu Gly*Glu Ile Ala Ala Gin Arg Gly Ala His Val Ser 225 230 235 240 Ile Val Ala Gly Asn Ala Ala Giu Leu Pro Thr Pro Ala Gly Ala Giu 245 250 255 Ilie Val Pro Val Val Ser Thr Gin Asp Met Phe Asp Ala Val Gin Giu 260 265 270 Arg Ala Gly Gin Ser Asp Phe Ilie Val Met Ala Ala Ala Val Ala Asp 275 280 285 Phe Thr Pro Ala Ser Gin Ala Thr Ser Lys Leu Lys Lys Gly Ser Asp 290 295 300 Ser Asp Glu Asp Ala Leu Ser Thr Ile Ser Leu Val Glu Asn Pro Asp 305 310 315 320 Ile Leu Ala Thr Thr Val Lys Arg Arg Giu Ala Gly Giu LeU Asp Ser 325 330 335 Asn Pro Val Ilie Val Giy Phe Ala Ala Giu Thr Gly Asp Giu His Thr 340 345 350 Thr Ala Leu Giu Tyr Ala Arg Lys Lys Leu.Gin Lys Lys Gly Cys Asp 355 360 365 Leu Leu Met Cys Asn Giu Val Gly Met Gly Lys Val Phe Gly Gin Lys 370 375 380 His Asn Giu Gly Trp Ile Leu Asp Ala His Gly Gly Val Val Asp Val 385 390 395 400 Glu His Gly Ser Lys Ile Giu Val Ala Ala Gin Ile Trp, Asp Ala Ala 405 410 415 Leu Ala Tyr Arg Giu Val 420 <210> 627 <211> 1092 <212> DNA <213> Corynebacterium giutamicui <220> <221> CDS <222> (101)..(1069) <223> RXA00581 <400> 627 gcatgagttt actcacgtgc ccacgtcttt tagccaccca ttgaagtgaa aaaataaccc cgatcacact agtggagtag ctaaggtgca caatggattc atg gca gag caa aac 115 Met Ala Giu Gin Asn 1 WO 01/00843 gct gca agc aca act ggt gtg aaa cct tcc cca cgc aca cca Ala Ala Ser Thr Thr Gly Val Lys Pro Ser Pro Arg Thr Pro PCTIiBOO/00923 agc Ser tca Ser atc Ile ctg Leu gca Ala ttt Phe gcc Al a gtg Val1 atc Ile 150 caa Gin gaa Giu ggc Gly tta Leu gac Asp 230 ccc Pro atg Met gga Gly tcc Ser gcc Ala gtc Val cga Arg gac Asp 135 cgc Arg cgt Arg gtc Val gaa Giu aac Asn 215 t tc Phe tac Tyr cct Pro gaa Giu cgt Arg acc Thr att Ile ctc Leu 120 ctc Leu cgc Arg gca Ala aac Asn t tc Phe 200 gtc Val1 agc Ser t tc Phe ctg Leu gac Asp cac His 75 ttC Phe gcg Ala gtt Val acc Thr atg Met 155 cgc Arg gtc Val1 gtc Val1 act Thr gta Val 235 gac Asp acc Thr ctc Leu 60 ctc Leu c tc Leu gga Gly c *tg Leu gac Asp 140 tcc Ser ttt Phe tac Tyr cgc Arg ggC Gly 220 ga t Asp 15 caa Gin gas Giu gtg Val gcg Ala tcc S er gcc Al a cgc Arg ctc Leu gga Gly gac Asp 175 ac c Thr gac Asp ttg Leu acc Thr tgg Trp gtc Val1 gca Ala gcc Ala 80 ccc Pro gtc Val1 tgg Trp, tat Tyr t tc Phe 160 gta Val gcg Ala att Ile atg Met gaa Giu 240 gat Asp aga Arg cga Arg ctt Leu ctt Leu gtg Val1 100 acc Thr Ccc Pro gaa Glu tac Tyr aaa Lys 180 gtt Val1 gaa Glu Ct t Leu aaa Lys ttc Phe aac Asn ggC Gly ccg Pro act Thr ccg Pro acc Thr cgc Arg cta Leu gac Asp 165 cc Leu cca Pro ggc Gly ttC Phe tgg Trp 245 tac atc gac cgc ttc ctc aaa ctc cgc gac act gca ttc cgt cgc ccc WO 01/00843 WO 0100843PCTJIBOO/00923 Tyr Ilie Asp Arg Phe Leu Lys Leu Arg Asp Thr Ala Phe Arg Arg Pro 250 255 260 ggt gcc cac ttc tcc cat tac gcc gac atg gct gat cca gag tcc atc 931 Gly Ala His Phe Ser His Tyr Ala Asp Met Ala Asp Pro Glu Ser Ile 265 270 275 gcc gtc gct cga gaa ctg tgg caa tcg atc aac ctg ccc aac ttg gtg 979 Ala Val Ala Arg Giu Leu Trp Gin Ser Ile Asn Leu Pro Asn Leu Val 280 285 290 gag aat att ctt ccc acc cga gtt cgc gcg tcg ttg gta ctg aaa aaa 1027 Giu Asn Ilie Leu Pro Thr Arg Val Arg Ala Ser Leu Val Leu Lys Lys 295 300 305 ggt agc gat cac ttg gtg gaa cgg gtg agg atg cgc aag atc 1069 Gly Ser Asp His Leu Val Giu Arg Val Arg Met Arg Lys Ile 310 315 320 taggggttct tgctggtttt gag 1092 <210> 628 <211> 323 <212> PRT <213> Corynebacteriun giutamicum <400> 628 Met Ala Giu Gin Asn Ala Ala Ser Thr Thr Gly Val Lys Pro Ser Pro 1 5 10 Arg Thr Pro Asp Phe Ser Pro Tyr Leu Asp Phe Asp Arg Ala Gin Trp 25 Arg Giu Leu Arg Asn Ser Met Pro Gin Val Leu Thr Gin Lys Giu Val 40 Ile Giu Leu Arg Gly Ile Gly Giu Asn Ile Asp Leu Ala Giu Val Ala 55 Giu Val Tyr Leu Pro Leu Ser Arg Leu Ile His Leu Gin Val Ala Ala 70 75 Arg Gin Gin Leu Thr Ala Ala Thr Giu Thr Phe Leu Gly Thr Ser Pro 90 Ser Ile Ser Val Pro Phe Val Ile Gly Val Ala Gly Ser Val Ala Val 100 105 110 Gly Lys Ser Thr Thr Ala Arg Leu Leu Gin Val Leu Leu Gin Arg Trp 115 120 125 Asn Ser His Pro Arg Val Asp Leu Val Thr Thr Asp Gly Phe Leu Tyr 130 135 140 Pro Gly Ala Giu Leu Ile Arg Arg Gly Leu Met Ser Arg Lys Gly Phe 145 150 155 160 WO 01/00843 Pro Giu SE Lys Ser GI Tyr Asp Ax is Leu Ile Va 210 Val Ser As 225 Asp Ile G1 Ala Phe Ax Asp Pro Gi 27 Leu Pro As 290 Leu Val Le 305 Arg Lys Ii Gin Gi u Giy Leu Asp 230 Tyr Giy Ala Giu Gly 310 Arg Val Giu Asn 215 Phe Ile Ala Val Asn 295 Ser Aia Asn Phe 200 Val Ser Asp His Ala 280 Ile Asp Arg Val1 Val Thr Val1 235 Leu His Leu Thr Val1 315 Thr His 190 Pro Thr Arg Arg Asp 270 Ser Arg Val1 PCT/1B00100923 Asp Vai 175 Thr Ala Asp Ile Leu Met Thr Giu 240 Asp Thr 255 Met Ala Ile Asn Ala Ser Arg Met 320 <210> 629 <211> 1023 <212> DNA <213> Corynebacterium glutamicun <22 0> <221> CDS <222> (101)..(1000) <223> RXS00838 <400> 629 tcgtctaata gtgctgccaa tccaccggcc attgatgact tgcttacaaa tcttatctgt gctcaggcaa gatagcaggt gtt ggc gct ggt gca gtt ggt gga tat ttc gga Val Gly Ala Gly Ala Val Gly Gly Tyr Phe Gly 15 tct ggt gca gat atc acg atg gtt gca cgt gga Ser Gly Ala Asp Ile Thr Met Val Ala Arg Gly 30 ttg aag tct aaa gga ctc cac atc aac gat gca Leu Lys Ser Lys Gly Leu His Ile Asn Asp Ala 893 cctttgtaga gaaggggtag atg aaa att gcg atc 115 Met Lys Ile Ala Ile 1 gcg ttg tta caa gaa 163 Ala Leu Leu Gin Glu cga aca tta gaa gcc 211 Arg Thr Leu Glu Ala aga ggc gaa cgc tac 259 Arg Gly Glu Arg Tyr WO 01/00843 gta cca att Val Pro Ile gta gtg atg Val Val Met gaa ctt ttg Giu Leu Leu aat tcg att Asn Ser Ile cgt gtg tgg Arg Val Trp 120 gcc tca gtg Ala Ser Val 135 tct ggt gaa Ser Giy Giu 150 att gac gga Ile Asp Gly gcc atg ttc Ala Met Phe caa tta ggt Gin Leu Gly 200 atg gaa gag Met Giu Glu 215 agc gat gcg Ser Asp Ala 230 aat tcg acg Asn Ser Thr gag ctt gag Giu Leu Glu ggt gtg aaa Gly Vai Lys 280 PCTIBOO/00923 gca Ala g'c t Ala ggg Gly tct Ser ggt Gly tac Tyr tct Ser ctg Leu 170 gag Giu ttg Leu gct Ala gag Giu tcg Ser 250 cag Gin ccg Pro gtt Val1 act Thr 75 ata Ile gct Ala gtg Val1 aag Lys agg Arg 155 cat His gtt Val1 cgt Arg gag Glu cgc Arg 235 atg Met aca Thr ctt Leu 45 agc Ser gca Ala gcg Ala cta Leu cgt Arg 125 ggc Giy ttc Phe gat Asp ggc Gly cat His 205 gct Ala a tg Met cgt Arg gca Ala gac Asp 285 cta Leu 65 tct Ser gtc Val1 agt Ser gtt Val1 tac Tyr 145 ctt Leu ga t Asp gc t Ala tcc Ser gg t Gly 225 ga t Asp gcg Ala gca Ala gct Ala aaa Lys tta Leu gcg Ala a tc Ile cat His 130 acg Thr gaa Giu gtg Val ttc Phe ctg Leu 210 gt t Val1 Arg gga Gly gcg Ala ggt Gly 290 gat Asp ga t Asp at t Ile ggt Gly 115 gag Glu ttt Phe cag Gin tgg Trp gtc Val1 195 gaa Glu gcg Ala atg Met gtg Val1 cac His 275 ctt Leu WO 01/00843 PCT/IB00/00923 aaa gaa gag gaa aat tea ctt tagggataga atcaagatcc atg 1023 Lys Glu Glu Glu Asn Ser Leu 295 300 <210> 630 <211> 300 <212> PRT <213> Corynebacterium glutamicum <400> 630 Met Lys Ile Ala Ile Val Gly Ala Gly Ala Val Gly Gly Tyr Phe Gly 1 5 10 Ala Leu Leu Gin Glu Ser Gly Ala Asp Ile Thr Met Val Ala Arg Gly 25 Arg Thr Leu Glu Ala Leu Lys Ser Lys Gly Leu His Ile Asn Asp Ala 40 Arg Gly Glu Arg Tyr Val Pro Ile Pro Ala Val Ala Ser Val Gin Glu 55 Leu Lys Asp Ala Asp Val Val Met Ile Ala Thr Lys Ala Leu Ser Arg 70 75 Ser Leu Asp Leu Ala Glu Leu Leu Gly Gly Ile Pro Ala Asn Ser Val 90 Val Ala Ile Thr Gin Asn Ser Ile Glu Ser Ala Asp Leu Ala Ala Lys 100 105 110 Ser Ile Gly Ala Asp Arg Val Trp Pro Gly Val Val Arg Gly Phe Phe 115 120 125 Val His Glu Gly Pro Ala Ser Val Ser Tyr Lys Gly Gly Pro Leu Ser 130 135 140 Tyr Thr Phe Gly Asp Ser Gly Glu Leu Ser Arg Gin Phe Ala Ser Thr 145 150 155 160 Leu Glu Gin Ala Gly Ile Asp Gly Val Leu His Pro Asp Ile Leu Val 165 170 175 Asp Val Trp Glu Lys Ala Met Phe Val Glu Val Phe Gly Gly Leu Gly 180 185 190 Ala Phe Val Glu Lys Gin Leu Gly Thr Leu Arg Thr His Phe Arg Ala 195 200 205 Ser Leu Glu Ala Leu Met Glu Glu Val Ala Glu Val Ala Arg Ala Ala 210 215 220 Gly Val Ala Leu Pro Ser Asp Ala Val Glu Arg Thr Met Asn Phe Ala 225 230 235 240 Asp Arg Met Pro Glu Asn Ser Thr Ser Ser Met Gin Arg Asp Leu Ala 245 250 255 WO 01/00843 WO 0100843PCT/IBOO/00923 Ala Gly Val Ala Ser Glu Leu Glu Ala 260 265 Ala Ala His Lys Val Gly Val Lys Thr 275 280 Ala Gly Leu Lys Leu Lys Glu Glu Glu 290 295 Gln Thr Gly Ala Ile Val Arg 270 Leu Ile Tyr Pro Leu His Asn Ser Leu 300 <210> 631 <211> 408 <212> DNA <213> Corynebacterium glutanicun <220> <222> <223>

CDS

(385) RXC 02238 <400> 631 ggcgcttagc caaaacatag agcggtaggg tatgcttatc cgattgagca acctttcccg ctcttaacac tactgtccat atacttttga aaaggtgtca gtg acc aac gtg Val Thr Asn Val aac gag acc aac Asn Glu Thr Asn acc aag gcc gtc ttc gat ccg cca gtg ggc att Thr Lys Ala Val Phe Asp Pro Pro Val Gly Ile acc gct cct Thr Ala Pro gcc ctc gtg Ala Leu Val atc gat gaa ctg Ile Asp Giu Leu ctg Leu gat aag gtc act Asp Lys Val Thr tCc aag tac Ser Lys Tyr atc ttc gca gcc Ile Phe Ala Ala aag cgt gcg cgc cag atc aac agc ttc Lys Arg Ala Arg Gln Ile Asn Ser Phe 45 tac cat Tryr His cag gca gat gag Gin Ala Asp Glu gta ttc gag ttc Val Phe Glu Phe gga cca ttg gtt Gly Pro Leu Val act Thr ccg cag cca ggc Pro Gin Pro Gly aag cca ctt tct att gct ctg cgt gag Lys Pro Leu Ser Ile Ala Leu Arg Giu aat gca ggt ctg Asn Ala Gly Leu gac cac gag gaa Asp His Glu Glu ggt Gly taaaagacct tataacttca <210> 632 <211> <212> PRT <213> Corynebacteriun glutamicun <400> 632 Val Thr Asn Val Ser Asn Glu Thr Asn Ala Thr Lys Ala Val Phe Asp WO 01/00843 WO 0100843PCT/IBOO/00923 1 Pro Val1 Gin Ile Ala Pro Thr Ile Giy Leu 10 Thr Ala Pro Pro Ile Asp Giu Leu Leu Ala Leu Val Ile Phe Ala Ala Lys Arg Tyr His Gin Ala Asp Giu Giy Val Phe 55 Thr Pro Gin Pro Gly Giu Lys Pro Leu 70 Asn Ala Gly Leu Leu Asp His Giu Giu Asp Ala Giu Ser Gly <210> 633 <211> 606 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (i0i)..(583) <223> R.XN03058 <400> 633 acgagcttcc gctctgcaca agccgctaga agccccgcat gcccatttgg aatcacaaca ccgcatatcg gccatggctg agccctaatg tagagctcat tca aag ctc aaa Ser Lys Leu Lys 115 ggC Gly gac Asp att Ile atc Ile aac Asn ggc Gly caa tca Ser ctg Leu aat Asn acg Thr aaa Lys c tc Leu gct tcg ctt Ser Leu ggc cgc Gly Arg acc ttt Thr Phe atc gcg Ile Ala gtc cga Val Arg ggC Gly cat His cag Gin cac His cc t Pro tcc Ser agc tcC Ser i5 gtc Val: ccg Pro atg Met ggc Giy gac Asp ctc acc caa agc Thr Gin Ser aac gcg gaa Asn Ala Giu ggc aag ctc Gly Lys Leu tgg atc atc Trp Ile Ile atc aat agg t tC Phe cct Pro gat Asp gga Gly atc Ile gga Giy 100 caa Gin Ala Phe Leu Ser Arg Pro Ile Ser Leu Ile Asn Arg Giu Gin Gin WO 01/00843 WO 0100843PCTJIBOO/00923 105 110 115 atc tac ccc ttc cct acc aaa gaa ccc tca gag agt ctc cac gaa ata Ile Tyr Pro Phe Pro Thr Lys Giu Pro Ser Giu Ser Leu His Giu Ile 120 125 130 cga caa ctc acc aag cag ctc ctc cct cac cgc cgt att cgc cgt gga Arg Gin Leu Thr Lys Gin Leu Leu Pro His Arg Arg Ile Arg Arg Gly 135 140 145 atc cac ttc cga tac ctc ctc gag tgg aca aag cct taaacagccc Ilie His Phe Arg Tyr Leu Leu Giu Trp Thr Lys Pro 150 155 160 tataaaccaa aaa <210> 634 <211> 161 <212> PRT <213> Corynebacteriun glutainicum <400> 634 Vai Ser Lys Leu Lys Gly Ser Arg Ser 1 Asp Val1 Gin Al a Lys Ilie Asn Ser Arg 145 Pro Ser Asp Tyr Giu Leu Ser Giu 115 His Arg 5 Ala Leu Ala Leu Val1 Leu Gin Ile Giy Asp Ile Ile Asn 70 Gly Gin Ile Arg Ile 150 Gly Thr Ile Val1 Lys Leu 105 Phe Thr Arg Leu 10 Arg Phe Al a Arg Asn 90 Ser Pro Lys Tyr Leu Gin Giu Ser Ile 75 Lys Arg Thr Gin Leu 155 Val Ala Phe His Asn Ala Ile Giu 125 Leu Glu Gly His Gin His Pro Ser Ser 110 Pro Pro Trp, Ser Val1 Pro Met Giy Asp Leu Ser His Thr Gly Asp Thr Asn Gly Trp Ile Giu Arg Lys 160 <210> 635 <211> 606 <212> DNA <213> Corynebacteriun giutamicum WO 01/00843 WO 0100843PCTIBOOIOO923 <220> <221> CDS <222> (101). .(583) <223> FRXA02903 acgagcttcc gctctgcaca agccgctaga agccccgcat gcccatttgg aatcacaaca ccgcatatcg gccatggctg agccctaatg tagagctcat gtg tca aag ctc aaa 11 Val Ser Lys Leu Lys ggc tca aga tcg Gly Ser Arg Ser ctc gac gtc ggc Leu Asp.Vai Gly ggc gat cac tcc Gly Asp His Ser ttc gcc Phe Ala gac ctg gcc Asp Leu Ala cgc cag gtc gcg Arg Gin Val Ala gtc gat gtc gtg Val Asp Val Vai gat cct ctt Asp Pro Leu tac gat gcc Tyr Asp Ala att aat aca acc ttt gaa gaa ttc cag ccg acc caa Ile Asn Thr Thr Phe Giu Giu Phe Gin Pro Thr Gin atc acg Ile Thr ttc atc gcg tcc ctc cat cac atg aac Phe Ile Ala Ser Leu His His Met Asn gaa gaa gga ctt Giu Giu Giy Leu aac Asn aaa gca gtc cga Lys Aia Vai Arg ctc aat cct ggc Leu Asn Pro Giy aag ctc ctc atc Lys Leu Leu Ile ggc ctc gcc aaa Giy Leu Aia Lys aaa acc gcc tcc Lys Thr Ala Ser tgg atc atc tcc Trp Ilie Ile Ser gga cta Giy Leu 100 355 403 451 499 caa gct ttt Gin Ala Phe atc tac ccc Ilie Tyr Pro 120 tcc cga cca atc agc ctc atc aat agg Ser Arg Pro Ile Ser Leu Ile Asn Arg gaa caa caa Giu Gin Gin 115 ttc cct acc aaa Phe Pro Thr Lys gaa Giu 125 ccc tca gag agt ctc cac gaa ata Pro Ser Giu Ser Leu His Giu Ile 130 cga caa Arg Gin 135 ctc acc aag cag Leu Thr Lys Gin ctc Leu 140 ctc cct cac cgc cgt att cgc cgt gga Leu Pro His Arg Arg Ilie Arg Arg Gly 145 cac ttc cga tac His Phe Arg Tyr ctc gag tgg aca Leu Giu Trp Thr aag cct Lys Pro 160 taaacagccc tataaaccaa aaa <210> 636 <211> 161 <212> PRT <213> Corynebacterium giutamicum 606 WO 01/00843 <400> 636 Val Ser Lys 1 Asp His Ser Val Val Asp Gin Ser Tyr Ala Glu Giu Lys Leu Leu Ile Ile Ser Asn Arg Giu 115 Ser Leu His 130 Arg Ilie Arg 145 Pro PCT/IBOOIOO923 Leu Phe Pro Asp Giy Ile Gly 100 Gin Giu Arg Giy Asp Ile Ile Asn 70 Giy Gin Ile Arg Ile 150 Arg Ala Thr 40 Phe Ala Al a Phe Pro 120 Leu Phe Ser Gly Thr Ile Val1 Lys Leu 105 Phe Thr Arg Leu Gin Glu Ser Ile 75 Lys Arg Thr Gin Leu 155 Val1 Ala Phe His Asn Ala Ile Giu 125 Leu Glu Ser Val1 Pro Met Giy Asp Leu Ser His Thr Gly Asp Thr Asn Giy Trp Ile Giu Arg Lys 160 <210> 637 <211> 783 <212> DNA <213> Corynebacterium giutainicum <220> <221> CDS <222> (101)..(760) <223> RXA00166 <400> 637 ggcgtttagc gatcttcaac atcgagcaac cagcgccagc gacttatcac gatgtccgac ctggatatcc ggctgaggcc ggg ttt ggc cga gtc ctg gat gtc ggt gca ggt Gly Phe Giy Arg Val Leu Asp Val Gly Ala Gly 15 agt gag cta aca gct gat cag gtc cta gcc ctt Ser Giu Leu Thr Ala Asp Gin Val Leu Ala Leu 30 atg ttg cgg gtg ttt cgc tcc gcg ctt ccg gcg 900 gcttttaccc aaggcagcac gtg gag tta gcc cgt 115 Val Giu Leu Ala Arg 1 acc gga aaa cta acc 163 Thr Gly Lys Leu Thr gat cca agc atg gac 211 Asp Pro Ser Met Asp gtt ccc tgc tgg caa 259 WO 01/00843 PCTJIBOO/00923 met Leu Arg Val Phe Arg Ser Ala Leu Pro Ala Val Pro Cys Trp Gin 45 gcg aca gca gaa cac aca gga ata cgt gac aac gcg gtt gat ctg att 307 Ala Thr Ala Glu His Thr Gly Ilie Arg Asp Asn Ala Val Asp Leu Ile 60 acg tgc gca caa acg tgg cat tgg gtt gac gtg acg gct gcc tca gcg 355 Thr Cys Ala Gin Thi- Trp, His Ti-p Val Asp Val Thr Ala Ala Ser Ala 75 80 gaa ttt gat cgg gtg att gca cct gag ggt gca gtc ctg ctc gtg tgg 403 Giu Phe Asp Arg Val Ilie Ala Pro Giu Gly Ala Val Leu Leu Val Ti-p 95 100 aat aac ctg gac acc tcc atc gcg tgg gta cac cga ctc agt cgc att 451 Asn Asn Leu Asp Thr Ser Ile Ala Trp, Val His Arg Leu Ser Arg Ile 105 110 115 atg cat gcc ggc gat gta ctc aag ccg gga ttc acc cca gaa acc gca 499 Met His Ala Gly Asp Val Leu Lys Pro Gly Phe Thr Pro Giu Thr Ala 120 125 130 gct ccc tgg ata att gat cga gaa att cgc acc acg tgg aat cag cac 547 Ala Pro Trp Ilie Ile Asp Arg Giu Ile Arg Thr Thr Trp Asn Gin His 135 140 145 ctc acc cct gaa gaa atc atc cag ctc gct cac acg agg tcc tac tgg 595 Leu Thr Pro Giu Gu Ilie Ile Gin Leu Ala His Thr Arg Ser Tyr Trp 150 155 160 165 tta aac gcg tca gag aaa atc aaa gag cgt gtt gat cag aac ctt cag 643 Leu Asn Ala Ser Giu Lys Ile Lys Giu Arg Val Asp Gin Asn Leu Gin 170 175 180 tgg tat ctc tac gag cat ttg ggt ttc agt ccc gac aat cca gtg gaa 691 Ti-p Tyr Leu Tyr Glu His Leu Gly Phe Ser Pro Asp Asn Pro Val Giu 185 190 195 ctt ccc tat cgc tgt gat gca ttt tta ctt tca cgt tcc ggt acc ctg 739 Leu Pro Tyr Arg Cys Asp Ala Phe Leu Leu Ser Arg Ser Gly Thi- Leu 200 205 210 gca ggc aga tct tcc aat ctt taggagccct cgccatgtac ctg 783 Ala Gly Arg Ser Ser Asn Leu 215 220 <210> 638 <211> 220 <212> PRT <213> Corynebacteriun glutamicum <400> 638 Val Giu Leu Ala Arg Gly Phe Gly Arg Val Leu Asp Val Gly Ala Gly 1 5 10 Thi- Gly Lys Leu Thi- Ser Giu Leu Thr Ala Asp Gin Val Leu Ala Leu 25 Asp Pro Ser Met Asp Met Leu Arg Val Phe Arg Ser Ala Leu Pro Ala 901 WO 01/00843 WO 0100843PCT/iBOO/00923 40 Val Pro Cys Trp Gin Ala Thr Ala Giu His Thr Giy Ilie Arg Asp Asn 55 Ala Val Asp Leu Ilie Thr Cys Ala Gin Thr Trp His Trp, Val Asp Val 70 75 Thr Ala Ala Ser Ala Giu Phe Asp Arg Val Ile Ala Pro Giu Gly Ala 90 Val Leu Leu Val Trp Asn Asn Leu Asp Thr Ser Ilie Ala Trp, Val His 100 105 110 Arg Leu Ser Arg Ilie Met His Ala Gly Asp Val Leu Lys Pro Gly Phe 115 120 125 Thr Pro Glu Thr Ala Ala Pro Trp Ile Ile Asp Arg Giu Ile Arg Thr 130 135 140 Thr Trp Asn Gin His Leu Thr Pro Glu Gu Ile Ile Gin Leu Ala His 145 150 155 160 Thr Arg Ser Tyr Trp Leu ASP Ala Ser Giu LYS Ilie Lys Glu Arg Val 165 170 175 Asp Gin Asn Leu Gin Trp Tyr Leu Tyr Glu His Leu Giy Phe Ser Pro 180 185 190 Asp Asn Pro Val Giu Leu Pro Tyr Arg Cys Asp Ala Phe Leu Leu Ser 195 200 205 Arg Ser Gly Thr Leu Ala Gly Arg Ser Ser Asn Leu 210 215 220 <210> 639 <211> 1392 <212> DNA <213> Corynebacterium giutanicun <2 <221> CDS <222> (101)..(1369) <223> RXA00633 <400> 639 aaagctgcgg taattaaaaa cacttagcgc caaaaattga acactgttca attaacctat tacactgcag atatacatcc aaaccaagtg acggaggaaa atg gaa aac ccc agc 115 met Giu Asn Pro Ser 1 ttg cgc gag ctt gat cac cga aac atc tgg cac ccg tat gcc gcg ccg 163 Leu Arg Glu Leu Asp His Arg Asn Ile Trp His Pro Tyr Ala Ala Pro 15 ggc gtg cgc aat aga ctc gtc acc aaa acc gat gga gtg ttt ttg acg 211 Gly Val Arg Asn Arg Leu Val Thr Lys Thr Asp Gly Val Phe Leu Thr 30 WO 01/00843 ctg gaa gat Leu Giu Asp PCTLBOO/00923 ggc agc acc gtg att gac gcg atg agc Gly Ser Thr Val Ile Asp Ala Met Ser tgg tgg tcg Trp Trp Ser gca att Ala Ile cat gga cac gga cac ccc cga ctg aaa His Gly His Gly His Pro Arg Leu Lys 60 gct Ala gcc gcc caa aaa Ala Ala Gin Lys caa Gin atc gac acc atg Ile Asp Thr Met agt Ser cac gtc atg ttt His Val Met Phe gga cta acc cac Gly Leu Thr His ccc gcc att aag Pro Ala Ilie Lys ctc Leu acc cac aaa ctc Thr His Lys Leu aat ctc act gga Asn Leu Thr Giy aat tcc Asn Ser 100 ttt gac cac Phe Asp His gcc atc aaa Ala Ilie Lys 120 ttt tat tcc gat Phe Tyr Ser Asp ggc tcg gtc tca Gly Ser Vai Ser gtg gag gtc Val Giu Vai 115 cac ccg gaa His Pro Giu atg gca ctg cag Met Ala Leu Gin tcc aaa gga caa Ser Lys Gly Gin ggc Giy 130 cgg aca Arg Thr 135 aaa ctc ctc acc Lys Leu Leu Thr cgg tcc ggc tac Arg Ser Giy Tyr cac His 145 gga gac aca ttc Gly Asp Thr Phe acc Thr 150 gcg atg agc gtg tgc gac cca gaa aat Ala Met Ser Val Cys Asp Pro Giu Asn 155 ggc Gly 160 atg cat agc ctc Met His-Ser Leu aaa ggc aca ctc Lys Gly Thr Leu gag cag att ttc Giu Gin Ilie Phe ccc gcc cca cca Pro Ala Pro Pro gtt cgg Val Arg 180 ggg tca tcg Gly Ser Ser ctt atc gac Leu Ile Asp 200 ccg Pro 185 cag gcg att tcc Gin Ala Ilie Ser tac ctg cgc agc Tyr Leu Arg Ser atg gaa ttg Met Giu Leu 195 atc gtc caa Ile Val Gin gag gcg gtc tcc Giu Ala Val Ser atc atc atc gaa Ile Ile Ile Giu ccg Pro 210 ggc gct Gly Ala 215 gga ggc atg cgc Gly Gly Met Arg cac gat gtc gca His Asp Val Ala ctc Leu 225 att gaa gga gtc Ile Giu Gly Val aca ctg tgc aag Thr Leu Cys Lys cac gat cgt ttc His Asp Arg Phe ttg Leu 240 atc gtc gat gaa Ile Val Asp Giu att Ile 245 gcc act ggt ttc Ala Thr Gly Phe cgc acc ggt gaa Arg Thr Gly Giu cta Leu 255 ttt gcc acg tta Phe Ala Thr Leu agc aat Ser Asn 260 ggc cta caa Gly Leu Gin gac atc atg tgt Asp Ile met Cys gtg Val1 270 ggc aag gcc ctc Gly Lys Ala Leu acc ggt gga Thr Gly Gly 275 ttc atg tcc ttc gcc gct act tta tgc acg gac aag gtg gct caa tta WO 01/00843 Phe Met Ser 280 PCTIiBOO/00923 atc agc 1027 Ile Ser 295 atg gct 1075 Met Ala 310 att gag 1123 Ile Giu ctt atc 1171 Leu Ile gtc cgg 1219 Val1 Arg aat gtc 1267 Asn Val 375 cgc ccc 1315 Arg Pro 390 tca gag 1363 Ser Giu ggg aaa 1392 Gly Lys acc Thr Phe Ala Ala Thr cca aat ggc gga Pro Asn Gly Gly 300 Cys Thr Asp gcg ctg atg Ala Leu Met gtt tcg cat Lys cac His 305 Val1 290 ggc Gly Ala Gin Leu ccc act ttt Pro Thr*Phe aat cct ctg gcc Asn Pro Leu Ala 315 acc ggc atg tgg Thr Gly Met Trp 330 gca ggc ctt tcc Ala Gly Leu Ser tgt gcg Cys Ala 320 cag aaa cag gta Gin Lys Gin Val 335 cca ctt caa cac Pro Leu Gin His aaa Lys ctt Leu gct tca tta gaa atc Ala Ser Leu Giu Ile 325 aga atc gaa gcc gaa Arg Ile Giu Ala Giu 340 cca ggg gtt gcc gat Pro Gly Val Ala Asp 345 gtt ctc Val Leu 360 ggc gcg att ggt Gly Ala Ile Gly 365 350 355 gtc atc gaa atg gaa caa aat gtc Val Ile Giu Met Giu Gin Asn Val gaa gaa gct act Giu Giu Ala Thr ttt gga cgc ttg Phe Gly Arg Leu 395 cag tgc gca cag Gin Cys Ala Gin 410 gct gca tta gat cac ggt gtg tgg atc Ala Ala Leu Asp His Gly Val Trp Ile 385 tat gtc atg cct cca tat atc acc acg Tyr Val Met Pro Pro Tyr Ile Thr Thr ctc Leu 400 atc tgc act gcg ctt Ile Cys Thr Ala Leu 415 cat gct gca gtt His Ala Ala Val 420 405 aaa Lys taaaccatgc catttttatt tgt <210> 640 <211> 423 <212> PRT <213> Corynebacterium glutamicun <400> 640 Met Glu Asn Pro Ser Leu Arg Giu Leu Asp His Arg Asn Ile Trp His 1 5 10 Pro Tyr Ala Ala Pro Gly Val Arg Asn Arg Leu Val Thr Lys Thr Asp 25 WO 01/00843 Gly Val Phe Leu Ser Ser Trp Trp Ala Ala Ala Gin Gly Leu Thr His Leu Thr Gly Asn 100 Val Ser Val Giu 115 Gin Gly His Pro 130 His Gly Asp Thr 145 Met His Ser Leu Ala Pro Pro Val 180 Arg Ser Met Giu 195 Glu Pro Ilie Val 210 Leu Ile Giu Gly 225 Ilie Val Asp Giu Ala Thr Leu Ser 260 Ala Leu Thr Gly 275 Lys Val Ala Gin 290 His Gly Pro Thr 305 Ala Ser Leu Giu Arg Ilie Giu Ala 340 PCTIBOO/00923 Thr Ser Lys Giu Ser Val1 Giu Phe Trp 165 Arg Leu Gin Val1 Ile 245 Asn Gly Leu Phe Ile 325 Giu Leu Ala Gin 70 Pro Phe Ala Arg Thr 150 Lys Giy Leu Gly Ala 230 Ala Giy Phe Ile Met 310 Ile Leu Giu Ile Ile Ala Asp Ile Thr 135 Aia Gly Ser Ilie Ala 215 Thr Thr Leu Met Ser 295 Ala Giu Ile Asp 40 His Asp Ile His Lys 120 Lys Met Thr Ser Asp 200 Gly Leu Gly Gin Ser 280 Thr Asn Thr Ala Giy Gly Thr Lys Val1 105 Met Leu Ser Leu Pro 185 Giu Gly Cys Phe Pro 265 Phe Pro Pro Gly Gly 345 Ser His Met Leu 90 Phe Ala Leu Val1 Pro 170 Gin Ala Met Lys Gly 250 Asp Ala Asn Leu Met 330 Leu Thr Gly Ser 75 Thr Tyr Leu Thr Cys 155 Giu Al a Val Arg Lys 235 Arg Ile Ala Gly Ala 315 Trp, Ser Ile Pro Val1 Lys Asp Ala 125 Arg Pro Ile Ser Ala 205 His Asp Gly Cys Leu 285 Gly Ala Lys Leu Asp Arg Met Leu Ser 110 Ser Ser Giu Phe Giu 190 Ile Asp Arg Giu Val1 270 Cys Ala Val1 Gin Gin 350 Ala Leu Phe Leu Gly Lys Gly Asn Ala 175 Tyr Ilie Val Phe Leu 255 Gly Thr Leu Ser Val 335 His Met Lys Giy Asn Ser Gly Tyr Gly 160 Pro Leu Ile Ala Leu 240 Phe Lys Asp Met His 320 Lys Leu Pro Gly Val Ala Asp Val Arg Val Leu Gly Ala Ile Gly Val Ile Giu WO 01/00843 WO 0100843PCTIAiB00I00923 355 360 365 Met Glu Gin Asn Val Asn Val Giu Giu Ala Thr Gin Ala Ala Leu Asp 370 375 380 His Gly Vai Ti-p Ile Arg Pro Phe Gly Arg Leu Leu Tyr Val Met Pro 385 390 395 400 Pro Tyr Ilie Thr Thr Ser Glu Gin Cys Ala Gin Ile Cys Thr Ala Leu 405 410 415 His Ala Ala Val Lys Gly Lys 420 <210> 641 <211> 795 <212> DNA <213> Corynebacterium giutamicun <22 0> <221> CDS <222> (101)..(772) <223> RXA00632 <400> 641 tggacgcttg ctctatgtca tgcctccata tatcaccacg tcagagcagt gcgcacagat ctgcactgcg cttcatgctg cagttaaagg gaaataaacc atg cca ttt tta ttt Met Pro Phe Leu Phe gtc Val1 ttg Leu cta Leu gaa Giu cc t Pro cag Gin cgc Arg agc Ser gtt Val gtc Val1 cgc Arg ctt Leu ttt Phe atc Ile ggg Giy gga Gly ggc Gly gag Giu cga Arg ct t Leu ggt Gly 110 aaa Lys cac His gag Giu gaa Giu cga Arg cgt Arg ggc Gly acc Thr gat Asp gga Gly ttt Phe gag Giu ggt Gly ctg Leu 115 163 211 259 307 355 403 451 499 gaa gat ttc acc ctg gca gat gtt gcc tcc gct ttg aat gca ccc tta WO 01/00843 WO 0100843PCT/EB00100923 Giu Asp Phe Thr Leu Ala Asp Vai Ala 120 125 gtg att gtg aca agc acc gga ttg gga Val Ile Val Thr Ser Thr Gly Leu Gly 135 140 agc gtt gag gca gca aac cgc cga gga Ser Val Giu Ala Ala Asn Arg Arg Gly 150 155 ggc ggt tcg atc cct caa aat cct gat Gly Gly Ser Ile Pro Gln Asn Pro Asp 170 gaa gaa ttt gag aga gtc acc ggc gtg Giu Giu Phe Giu Arg Val Thr Gly Val 185 190 gaa ggg ttg tca cgg gtg gag ggg ttc Giu Gly Leu Ser Arg Val Giu Gly Phe 200 205 gcc ctt gat gcc ttt aag aaa ccg ccg Ala Leu Asp Ala Phe Lys Lys Pro Pro 215 220 tcg <210> 642 <211> 224 <212> PRT <213> Corynebacterium giutarnicum <400> 642 Met Pro Phe Leu Phe Val Ser Gly Thr 1 Phe Ser Thr Ala Val Leu Val Arg Tyr Val Leu Pro Val Lys Leu Val Gin Thr Asp Ilie Phe Asn Ile Giu Arg Leu Thr Ala Arg Phe Lys Asp Pro Leu Ala Pro 70 Gly Val Giu Pro Ile Gin Phe Asp Gin Phe Asp Asp Pro Asp Arg Ile Ile Val 100 105 Leu Val Arg Leu Gly Giu Asp Phe Thr 115 120 Leu Asn Ala Pro Leu Val Ile Val Thr 907 Ala ctc Leu aca Thr 160 gct Ala ttt Phe gaa Giu agg Arg Leu Asn Ala Pro Leu 130 aac gct gct gaa tta 547 Asn Ala Ala Giu Leu 145 gtg ttg gga gtc ctc 595 Val Leu Gly Val Leu 165 acg atg ctt aat ctc 643 Thr Met Leu Asn Leu 180 tgg gga gct ttg ccg 691 Trp Gly Ala Leu Pro 195 aag caa tct tit ccg 739 Lys Gin Ser Phe Pro 210 tgatcgtgaa caccgtgcct 792 Thr Ala Giu Ile Leu Ile Giu Ala Thr WO 01/00843 130 Asn Ala Ala Giu Leu Ser 145 150 Val Leu Gly Val Leu Gly 165 Thr Met Leu Asn Leu Giu 180 Trp Giy Ala Leu Pro Giu 195 Lys Gin Ser Phe Pro Ala 210 PCTJIBOO/00923 Glu Ala Ala Asn 155 Ser Ile Pro Gin 170 Phe Glu Arg Val 185 Leu Ser Arg Vai 200 Asp Aia Phe Lys 140 Arg Arg Gly Asn Pro Asp Thr Gly Vai 190 Giu Giy Phe 205 Lys Pro Pro 220 Thr 160 Ala Phe Giu Arg <210> 643 <211> 1125 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(1102) <223> RXA00295 <400> 643 gtttaaggca cgtgtcattt tgcatgcgcg tgcccaattc ttcttaagtg tgctcaattt ttaagcgcgc agttattgac aaccagcctc taggagatcc atg acc atc ccc ggc acc Thr ctt Leu atc Ilie ggg Gly tgc Cys ccg Pro gac Asp cag Gin ttg Leu atc Ile gat Asp tcg Ser acc Thr cag Gin atg Met gag Giu tgt Cys gtg Val1 Met gaa Giu ttg Leu Cgg Arg ctc Leu ggg Giy ctg Leu Thr cag Gin cc t Pro ttg Leu aaa Lys ttg Leu gt t Val Giy ggc Gly caa Gin tgt Cys ggt Gly tcg Ser gct Ala WO 01/00843 aaa cag acc Lys Gin Thr PCTJIBOO/00923 gca aaa act Ala Lys Thr gct acc gaa ttc tgt atc Ala Thr Glu Phe Cys Ile 105 110 gtc aag Val Lys ctc gcg Leu Ala 135 acg tta Thr Leu 150 cgc tac Arg Tyr gtc acc Val Thr gca gaa Ala Glu gaa act Giu Thr 215 gat ccg Asp Pro 230 cca ttt Pro Phe att ggt Ile Gly ggc ggt Gly Gly ctg gga 1027 Leu Gly 295 ggc cgc 1075 Gly Arg 310 ccc atc 1122 ggg G ly 120 at t Ile aat Asn aac Asn act Thr gct Ala 200 tta Leu cac His gcc Ala gcg Ala cgc Arg 280 ggc Gly cca Pro aaa cct Pro cac His aag Lys cat His cat Hius 185 gga Gly gag Giu gaa Glu gat Asp t tc Phe 265 gag Glu atc Ile atg Met gtc ga t Asp tct Ser gaa Glu aat Asn 170 aca Thr atg Met cag Gin gtc Val agg Arg 250 cgc Arg ctg Leu aat Asn gaa Glu ctt gag Glu gaa Glu cag Gin 155 ttg Leu tgg Trp, gaa Giu cgc Arg ccc Pro 235 gaa Glu ctt Leu act Thr gcg Ala gat Asp 315 aat agg Arg gtt Val 140 gtg Val gaa Giu gaa Giu gtc Val gcc Ala 220 atg Met ttg Leu gcg Ala ttg Leu atg Met 300 gac Asp aag atg acc cag Met Thr Gin att gaa gtc Ile Glu Val cgc ctc gct Arg Leu Ala 160 gcg cgt tcc Ala Arg Ser 175 cgc cgc gaa Arg Arg Giu 190 tcc ggc gga Ser Gly Gly ttt gcc gtg Phe Ala Val ttc ctt gat Phe Leu Asp 240 gac agc cgt Asp Ser Arg 255 cct cac acc Pro His Thr 270 gac aag ggt Asp Lys Gly gtc gga aac Val Gly Asn gac atg atg Asp Met Met 320 atc taagaag( ctg Leu gca Ala 145 gct Ala tat Tyr act Thr atc Ile cag Gin 225 cc t Pro gac Asp atg Met tcc Ser t ac Tyr 305 gat gag Glu 130 gca Ala gcc Ala ttc Phe t tg Leu tta Leu 210 ctg Leu cgc Arg gc t Al a ct t Leu gag Glu 290 ctg Leu cgt gtc gcc Val Ala 115 gaa gca Glu Ala tcg atc Ser Ile ggc gtg Gly Val cct gaa Pro Glu 180 cgc ctg Arg Leu 195 gga atg Gly Met gcg gag Ala Giu ccg ggc Pro Gly ctg cgc Leu Arg 260 cgt ttt Arg Phe 275 caa gcc Gln Ala act acg Thr Thr ctc cag gca Al a gtc Val1 gga Gly cac His 165 gtt Val1 gtg Val1 ggc Gly ctt Leu acc Thr 245 tct Ser gct Ala c tc Leu ctc Leu ctg Asp Arg Leu Gln Leu :ac gcgcatgaac WO 01/00843 WO 0100843PCT/IBOO/00923 Pro Ile Lys Val Leu Asn Lys Val Ile 330 gac 1125 <210> 644 <211> 334 <212> PRT <213> Corynebacterium giutamicun <400> 644 Met Thr Ilie Pro Gly Thr Ile Leu Asp Thr Ala Arg Thr Gin Val Leu 1 5 10 Glu Gin Gly Ile Gly Leu Asn Gin Gin Gin Leu Met Giu Vai Leu Thr 25 Leu Pro Giu Giu Gin Ile Pro Asp Leu Met Giu Leu Ala His Gin Vai 40 Arg Leu Lys Trp Cys Giy Giu Giu Ile Glu Vai Giu Gly Ile Ile Ser 55 Leu Lys Thr Giy Giy Cys Pro Glu Asp Cys His Phe Cys Ser Gin Ser 70 75 Giy Leu Phe Giu Ser Pro Val Arg Ser Val Trp Leu Asp Ile Pro Asn 90 Leu Vai Giu Ala Ala Lys Gin Thr Ala Lys Thr Gly Ala Thr Giu Phe .100 105 110 Cys Ilie Vai Ala Ala Vai Lys Gly Pro Asp Glu Arg Leu Met Thr Gin 115 120 125 Leu Giu Glu Ala Val Leu Ala Ilie His Ser Giu Val Giu Ile Glu Val 130 135 140 Ala Ala Ser Ilie Gly Thr Leu Asn Lys Giu Gin Val Asp Arg Leu Ala 145 150 155 160 Ala Ala Gly Val His Arg Tyr Asn His Asn Leu Giu Thr Ala Arg Ser 165 170 175 Tyr Phe Pro Giu Vai Val Thr Thr His Thr Trp Giu Giu Arg Arg Giu 180 185 190 Thr Leu Arg Leu Val Ala Giu Ala Gly Met Glu Val Cys Ser Gly Gly 195 200 205 Ile Leu Gly Met Giy Giu Thr Leu Giu Gin Arg Ala Giu Phe Ala Val 210 215 220 Gin Leu Ala Giu Leu Asp Pro His Giu Val Pro Met Asn Phe Leu Asp 225 230 235 240 Pro Arg Pro Gly Thr Pro Phe Ala Asp Arg Giu Leu Met Asp Ser Arg 245 250 255 WO 01/00843 Asp Ala Leu Met Leu Arg 275 Ser Glu Gin 290 Tyr Leu Thr 305 Asp Arg Leu Ser Ala Leu Leu Leu 325 Ile Gly Ala Phe 265 Gly Gly Arg Glu 280 Leu Gly Gly Ile 295 Gly Arg Pro Met 310 Pro Ile Lys Val Arg Leu Asn Glu Leu 330 PCTAIBOO/00923 Leu Ala Met Pro His Thr 270 Thr Leu Gly Asp Lys Gly 285 Ala Met Ile Val Gly Asn 300 Asp Asp Leu Asp Met Met 315 320 Asn Lys Val Ile <210> 645 <211> 1212 <212> DNA <213> Corynebacteriun giutamicun <22 0> <221> CDS <222> (101)..(1189) <223> RXA00223 <400> 645 gcgacctctt tgacatcgcc cctgcgctca tcgaagagat caacaagcgc aagtaggagt tttgaacact ttttatctgg 4ccatgcagc caccacacca atg cgt gag gtg gcc Met Arg Giu Val Ala gca gct gcg Ala Ala Ala tac ggt tcg Tyr Gly- Ser gaa att gct Giu Ile Ala gcg tcc ggc Ala Ser Gly gca tcg cct Ala Ser Pro att ctg gaa Ile Leu Glu ctc-atg ccg Leu Met Pro atg Met cgt Arg t tg Leu gag Glu aat Asn gtc Val1 ggt Gly cag Gin agc Ser gaa Glu ctc Leu tc t Ser gaa Glu cga Arg 110 1 aat Asn gat Asp gag *Giu cag Gin atc Ile ggg Gly tcc Ser ccc Pro tcg Ser gt t Val1 ggg Gly gag Giu gca Ala ttc Phe gcg Ala gc t Ala gtg Val1 t ta Leu cac His gag Glu gaa Glu 115 agt Ser cgt Arg ttt Phe ttc Phe ccc Pro gcg Ala 100 gcg Ala 115 163 211 259 307 355 403 451 WO 01/00843 gac aag cct gcc gcg gtt gcc act atg atg tgg gcg Asp Lys Pro Ala Ala Val Ala Thr Met Met Trp Ala PCTfBOO/00923 120 125 ggc gcg Gly Ala 135 ggc acg Gly Thr 150 gtc aat Val Asn aaa ttc Lys Phe cca gca Pro Ala cgt cca Arg Pro 215 tta cgc Leu Arg 230 gga ctt Gly Leu gta ctg Val Leu tcc ttc Ser Phe ttg cgg 1027 Leu Arg 295 cgt gcc 1075 Arg Ala 310 cgt ggt 1123 Arg Gly atc aag 1171 att Ile cc a Pro ttt Phe gg t Gly cct Pro 200 ggc Gly gaa Glu aaa Lys gtc Val1 cca Pro 280 atc Ile agc Ser gcc Ala gca gtt Val1 atc Ile 155 ctc Leu cgt Arg gta Val1 gat Asp gcc Ala 235 ctt Leu acc Thr gaa Glu tcc Ser ctt Leu 315 t tc Phe gcc tct Ser 140 gat Asp ggc Gly ggc G ly ttg Leu gtc Val 220 gag Glu ctc Leu gaa Glu ggc Gly aca Thr 300 ttg Leu acc Thr gtg gag Glu gcg Ala gcc Ala gtc Val1 cac His 205 gcc Ala ctt Leu gac Asp cca Pro gat Asp 285 ggt Gly gcc Ala ctc Leu atc t tc Phe gtt Val1 acc Thr ggc Gly 190 gga Gly ggc Gly gac Asp gcc Ala tcc Ser 270 agt Ser tcg Ser atg Met gga Gly gaa atc Ile cag Gin act Thr 175 ctg Leu ggt Giy gca Ala ggc Gly atc Ile 255 ctg Leu ttg Leu gcc Ala gga Gly aga Arg 335 gac gcc Ala gtc Val1 160 t tg Leu ctg Leu gg t Gly gc t Ala gaa Glu 240 ctc Leu cca Pro a tc I le tgc Cys att I le 320 acc Thr gta gcc Ala 145 gtt Val gct Ala ttg Leu cag Gin gcc Ala 225 gcc Alacac His gga Gly atg Met tcc Ser 305 tcc Ser acc Thr gtg aac aat gag Asn Asn Glu 130 gcg cag gcg Ala Gin Ala ggc cat ctg Gly His Leu gcc tcc gcg Ala Ser Ala 180 gtg agg cgc Val Arg Arg 195 gag cgc ggc Giu Arg Gly 210 acc gca gcc Thr Ala Ala acc cgc ctg Thr Arg Leu acc atc ccc Thr Ile Pro 260 cac ctg cat His Leu His 275 ctg ctc gac Leu Leu Asp 290 aac ggt gta Asn Gly Val gaa acc gac Giu Thr Asp act gaa gaa Thr Glu Glu 340 acc agg gct acc Thr t cc Ser ccg Pro 165 cac His tca Ser atc Ile gca Ala cgc Arg 245 aac Asn ctc Leu tcc Ser aac Asn gcc Ala 325 tcc Ser cgt 499 WO 01/00843 PCTIIBOO/00923 Ile Lys Ala Val Ile Ala Val Ile Glu ASP Val Val Thr Arg Ala Arg 345 350 355 act gcg gga atg gct ttt tagcgaccgt aaatcgcata gtg 1212 Thr Ala Gly Met Ala Phe 360 <210> 646 <211> 363 <212> PRT <213> Corynebacterium glutamicun <400> 646 Met Arg Glu Val Ala Ala Ala Ala Trp 1 Asn Asp Glu Gin Ile Gly Ser Ala Ala 145 Val Ala Leu Gin Ala 225 Ala Pro Ser Val1 Gly Giu Ala Phe Asn 130 Ala Gly Ala Val1 Glu 210 Thr Thr Ala Ala Val1 Leu His Giu Giu 115 Asn Gin His Ser Arg 195 Arg Ala Arg Ser Arg Phe Phe Pro Ala 100 Ala Giu Ala Leu Ala 180 Arg Gly Ala Leu 5 Gin Giu Thr His Gly Giu Leu Thr Ser Pro 165 His Ser Ile Ala Arg Tyr Giu Ala Ala 70 Ile Leu Asp G ly Gly 150 Val1 Lys Pro Arg Leu 230 Gly Gly Ser Ile Ala 40 Ser Gly 55 Ser Pro Leu Giu Met Pro Lys Pro 120 Ala Ile 135 Thr Pro Asn Phe Phe Gly Ala Pro 200 Pro Gly 215 Arg Giu Leu Lys Gly 25 Ser Thr Leu Thr- Ile 105 Ala Gin Thr Asp Gly 185 Ser Thr Ala Lys 913 Met 10 Arg Leu Giu Asn Val1 90 Gly Ala Pro His Giu 170 Pro Ala Leu Val1 Met Giu Lys Leu Ala Arg 75 Lys Pro Val1 Val1 Ile 155 Leu Arg Val1 Asp Ala 235 Leu Asn Ala Gly Asp Ile Ala Asp Ala Ser 140 Asp Gly Gly Leu Val1 220 G iu Leu Ala Arg Cys Asn Ile Leu Gly Thr 125 Giu Ala Ala Val His 205 Ala Leu Asp Gin Ser Giu Leu Ser Giu Arg 110 Met Phe Val1 Thr Gly 190 Gly Gly Asp Ala Ala Val Pro Ala Thr Leu Val1 Met Ile Gin Thr 175 Leu G ly Ala Gly Ile Leu Ala Ile Val1 Pro Gly Ser Trp, Ala Val1 160 Leu Leu Gly Ala Glu 240 Leu WO 01/00843 His Gly Met Ser 305 Ser Thr Val1 Ile Leu 275 Leu Gly Thr Glu Arg 355 Pro 260 His Asp Val1 Asp Glu 340 Ala 245 Asn Leu Ser Asn Ala 325 Ser Arg Val1 Pro 280 Ile Ser Ala Ala Gly 360 250 Thr Al a Ala Val1 Arg 330 Ile Ala Thr Glu Ser Leu 315 Phe Ala Phe PCTIIBOO/00923 255 Leu Pro Lieu Ile Ala Cys Gly Ile 320 Axg Thr 335 Asp Val <210> 647 <211> 1197 <212> DNA <213> Coryriebacterium glutamicum <22 0> <221> CDS <222> (101)..(1174) <223> RXN00262 <400> 647 acaccgcggg aaagattgca tcaaccggtg tcgacgtcat attctgtgca tgcacttgac ctaggactcg atattttcta aat Asn cct Pro gga Gly cgc Arg tca S er cgc Arg ggc Gly ctg Leu aca Thr atc Ile gca Ala tca Ser gct Ala acg Thr gcg Ala 80 ttccgttgga gcgcttaccc atg ctc tac ctt gat Met Leu Tyr Leu Asp 1 ctt gag gcc atg tgg Leu Glu Ala Met Trp, agt ccc cat gag gtg Ser Pro His Glu Val cga act cgg gtg gcc Arg Thr Arg Val Ala ttt acg tcg ggt gga Phe Thr Ser Gly Gly tgc tta gct aat cct Cys Leu Ala Asn Pro WO 01/00843 cgt ggc cgg Arg Giy Arg gaa act got Glu Thr Ala cta too coo Leu Ser Pro* 120 goa gtc agg Ala Val Arg 135 gag gtg gga Giu Val Giy 150 acg oct ttt Thr Pro Phe ttg gga gtt Leu Gly Vai aaa ggg att Lys Gly Ile 200 cat ggc ggc His Gly Giy 215 gcg ggg got Ala Gly Ala 230 tcc tat cca Ser Tyr Pro gga gca cac Gly Ala His tot ttt ctc Ser Phe Leu 280 gaa cgc caa 1027 Glu Arg Gin 295 gag gta tcc 1075 Giu Vai Ser 310 oac His got Ala 105 gat Asp ocg Pro acc Thr cao His gao Asp 185 gga Gly ggc Gly atc Ile gat Asp otg Leu 265 t to Phe ggo Gly oat His ato Ile ott Leu act Thr ao 0 Thr cag Gin 155 gat Asp t ta Leu tta Leu gaa Giu ttt Phe 235 ggc Gly gga G ly ago Ser gtg Val1 ttg Leu 315 acc Thr gaa Giu ggg Giy aca Thr 140 cog Pro gca Ala agt Ser tgg Trp aaa Lys 220 gc Ala gaa Glu cat His ata Ile tgc Cys 300 ctg Leu agt Ser ato Ile 115 oto Leu gc Ala gta Val1 aco Thr ggt Gly 195 cog Pro gaa Giu agg Arg aoct Thr gga Gly 275 otg Leu ggt Gly PCTIIBOO/00923 gto ota 403 Val Leu 100 acc tao 451 Thr Tyr ogo aaa 499 Arg Lys aao aat 547 Asn Asn ago agt 595 Ser Ser 165 ttt gao 643 Phe Asp 180 gog cot 691 Ala Pro gta ato 739 Val Ile aao gtt 787 Asn Val gog gaa 835 Ala Giu 245 ato cog 883 Ile Pro 260 cac gca 931 His Ala gaa ttg 979 Giu Leu too gga Ser Gly gag gag gat caa gca Gly Leu Giu Giu Asp Gin Ala 320 325 WO 01/00843 WO 0100843PCT/IBOO/00923 cga acg gct gtg cgc tgt act ttt agt aca aca cac agc cgt gaa gat 1123 Arg Thr Ala Val Arg Cys Thr Phe Ser Thr Thr His Ser Arg Glu Asp 330 335 340 gcg ctc gtg gca gcc tct gct ctt aaa tcc gcg gtc gcc tta atc aga 1171 Ala Leu Val Ala Ala Ser Ala Leu Lys Ser Ala Val Ala Leu Ile Arg 345 350 355 ggg tgacgctagt cagaggttta cgg 1197 Gly <210> 648 <211> 358 <212> PRT <213> Corynebacterium glutamicum <400> 648 Met Leu Tyr Leu Asp Asn Ala Ala Thr Thr Ser Val Arg Asn Glu Ala 1 5 10 Leu Glu Ala Met Trp Pro Tyr Leu Thr Gly Ala Phe Gly Asn Pro Ser 25 Ser Pro His Glu Val Gly Arg Leu Ala Ser Ala Gly Leu Giu Asp Ala 40 Arg Thr Arg Val Ala Arg Ilie Ile Gly Gly Arg Pro Thr Gin Val Thr 55 Phe Thr Ser Gly Gly Ser Glu Ala Asn Asn Leu Ala Ile Lys Gly Ala 70 75 Cys Leu Ala Asn Pro Arg Gly Arg His Leu Ile Thr Thr Pro Ile Giu 90 His Asp Ser Val Leu Glu Thr Ala Ala Tyr Leu Glu Arg Phe His Asp 100 105 110 Phe Glu Ile Thr Tyr Leu Ser Pro Asp His Thr Gly Leu Ile Ser Pro 115 120 125 Glu Gly Leu Arg Lys Ala Val Arg Pro Asp Thr Thr Leu Ile Ser Ile 130 135 140 Gly Tyr Ala Asn Asn Glu Val Gly Thr Ile Gin Pro Ile Ala Glu Leu 145 150 155 160 Ala Ala Val Ser Ser Thr Pro Phe His Thr Asp Ala Val Gin Ala Ala 165 170 175 His Leu Thr Phe Asp Leu Gly Val Asp Ala Leu Ser Leu Ser Gly His 180 185 190 Lys Phe Gly Ala Pro Lys Gly Ile Gly Val Leu Trp Ser Lys Leu Pro 195 200 205 WO 01/00843 WO 0100843PCT/IBOO/00923 Leu Glu Pro Val Ile His Gly Gly Gly Gin Glu Lys Gly Arg Arg Ser 210 215 220 Gly Thr Giu Asn Val Ala Gly Ala Ile Ala Phe Ala Thr Ala Leu Glu 225 230 235 240 Leu Ala Arg Ala Glu Ser Tyr Pro Asp Leu Gly Glu Phe Ile Glu Glu 245 250 255 Val Leu Thr Ile Pro Gly Ala His Leu Thr Gly His Pro Arg Met Arg 260 265 270 Ilie Asp Gly His Ala Ser Phe Leu Phe Asp Ser Ile Gly Ser Giu Thr 275 280 285 Val Leu Leu Glu Leu Giu Arg Gin Gly Ile Val Cys Ser Pro Gly Ser 290 295 300 Ala Cys Gly Ser Gly Giu Val Ser His Val Leu Leu Ala Leu Gly Leu 305 310 315 320 Glu Giu Asp Gin Ala Arg Thr Ala Val Arg Cys Thr Phe Ser Thr Thr 325 330 335 His Ser Arg Glu Asp Ala Leu Val Ala Ala Ser Ala Leu Lys Ser Ala 340 345 350 Val Ala Leu Ile Arg Gly 355 <210> 649 <211> 920 <212> DNA <213> Corynebacterium glutamicuxn <22 0> <221> CDS <222> (897) <223> FRXA00262 <400> 649 cacacaggtg acgtttacgt cgggtggatc agaagccaac aacctcgctt atcaaaggag cgtgcttagc taatcctagtg gcc ggg cac ctc atc acc acc ccg atc gag ill Val Ala Gly His Leu Ile Thr Thr Pro Ile Glu 1 5 cat gac agt gtc cta gaa act gct gct tat ctt gaa agg ttt cat gat 159 His Asp Ser Val Leu Giu Thr Ala Ala Tyr Leu Glu Arg Phe His Asp 20 ttc gag atc acc tac cta tcc ccc gat cac act ggg ctg atc tcc ccg 207 Phe Giu Ile Thr Tyr Leu Ser Pro Asp His Thr Gly Leu Ile Ser Pro 35 gag ggt ctc cgc aaa gca gtc agg ccg gac acc aca ttg atc agc att 255 Giu Gly Leu Arg Lys Ala Val Arg Pro Asp Thr Thr Leu Ile Ser Ile 50 WO 01/00843 WO 0100843PCT/IBOO/00923 ggt Gly tat gcc aac aat Tyr Ala Asn Asn gag Giu gtg gga acc att Val Gly Thr Ile ccg ata gct gag Pro Ile Ala Giu gcg gcg gta agc Ala Ala Val Ser acg cct ttt cac Thr Pro Phe His gat gca gtg caa Asp Ala Val Gin gct gca Ala Ala cat tta acc His Leu Thr aaa ttc ggt Lys Phe Gly 110 gac ttg gga gtt Asp Leu Gly Val gcg tta agt ttg Ala Leu Ser Leu tcg ggt cat Ser Gly His 105 gcg cct aaa ggg att gga gtg tta tgg Ala Pro Lys Gly Ile Gly Val Leu Trp tca aag ctt ccc Ser Lys Leu Pro 120 ggg cgg cgt agt Gly Arg Arg Ser ctg gag Leu Glu 125 ccg gta atc cat Pro Val Ile His ggc Gly 130 ggc ggc cag gaa Gly Gly Gin Glu ggc Gly 140 acg gaa aac gtt Thr Glu Asn Val gcg Ala 145 ggg gct atc gcc Gly Ala Ile Ala gcc act gcc ttg Ala Thr Ala Leu ttg gcc agg gcg Leu Ala Arg Ala tcc tat cca gat Ser Tyr Pro Asp ggc gaa ttc atc Gly Giu Phe Ile gag gaa Glu Glu 170 gtt ctc act Val Leu Thr att gat gga Ile Asp Gly 190 ccg gga gca cac Pro Gly Ala His act gga cat cct Thr Gly His Pro agg atg cgc Arg Met Arg 185 tct gaa act Ser Glu Thr cac gca tct ttt His Ala Ser Phe ctc Leu 195 ttc gac agc ata Phe Asp Ser Ile gtt ctt Val Leu 205 ctg gaa ttg gaa Leu Glu Leu Glu caa ggc att gtg Gin Gly Ile Val tgc tcc cct ggt Cys Ser Pro Gly 215 ctg gcg ttg ggg Leu Ala Leu Gly tct Ser ctt Leu 235 gcc Ala 220 tgt ggt tcc gga Cys Gly Ser Gly gta tcc cat gtg Val Ser His Val ttg Leu 230 gag gag gat caa Glu Giu Asp Gin cga acg gct gtg Arg Thr Ala Val cgc Arg 245 tgt act ttt agt Cys Thr Phe Ser aca aca Thr Thr 250 cac agc cgt His Ser Arg gtc gcc tta Vai Ala Leu 270 gat gcg ctc gtg Asp Ala Leu Val gca Ala 260 gcc tct gct ctt Ala Ser Ala Leu aaa tcc gcg Lys Ser Ala 265 atc aga ggg Ile Arg Gly tgacgctagt cagaggttta cgg <210> <211> <212> <213> 650 273

PRT

Corynebacteriui giutamicum WO 01/00843 WO 0100843PCTEBOOIOO923 <400> 650 Val Ala Gly His Leu Ilie Thr Thr Pro Ile Glu His Asp Ser Val Leu 1 5 10 Giu Thr Ala Ala Tyr Leu Glu Arg Phe His Asp Phe Glu Ile Thr Tyr 25 Leu Ser Pro Asp His Thr Gly Leu Ile Ser Pro Glu Gly Leu Arg Lys 40 Ala Val Arg Pro Asp Thr Thr Leu Ile Ser Ile Gly Tyr Ala Asn Asn 55 Glu Val Gly Thr Ile Gin Pro Ile Ala Giu Leu Ala Ala Val Ser Ser 70 75 Thr Pro Phe His Thr Asp Ala Val Gin Ala Ala His Leu Thr Phe Asp 90 Leu Giy Val Asp Ala Leu Ser Leu Ser Gly His Lys Phe Gly Ala Pro 100 105 110 Lys Gly Ile Gly Val Leu Trp Ser Lys Leu Pro Leu Giu Pro Val Ile 115 120 125 His Gly Gly Gly Gin Giu Lys Gly Arg Arg Ser Gly Thr Giu Asn Vai 130 135 140 Ala Giy Ala Ile Ala Phe Ala Thr Ala Leu Glu Leu Ala Arg Ala Giu 145 150 155 160 Ser Tyr Pro Asp Leu Gly Glu Phe Ile Giu Giu Val Leu Thr Ile Pro 165 170 175 Gly Ala His Leu Thr Gly His Pro Arg Met Arg Ile Asp Gly His Ala 180 185 190 Ser Phe Leu Phe Asp Ser Ile Gly Ser Giu Thr Val Leu Leu Giu Leu 195 200 205 Giu Arg Gin Gly Ile Val Cys Ser Pro Gly Ser Ala Cys Gly Ser Gly 210 215 220 Giu Val Ser His Val Leu Leu Ala Leu Gly Leu Giu Giu Asp Gin Ala 225 230 235 240 Arg Thr Ala Val Arg Cys Thr Phe Ser Thr Thr His Ser Arg Giu Asp 245 250 255 Ala Leu Val Ala Ala Ser Ala Leu Lys Ser Ala Val Ala Leu Ile Arg 260 265 270 Gly <210> 651 <211> 1296 <212> DNA <213> Corynebacterium glutaxnicum WO 01/00843 WO 0100843PCTJJ!BOO/00923 <220> <221> CDS <222> (101)..(1273) <223> RXN00435 <400> 651 cgacaggtga attcatgcac gtttgagtgt cccgtgtgtg gggtaatgtt gtccaagaga gtggaaggaa atgctgtggc ggttgaaagg agtgcctttc gtg ggt ttt gat gtg Val Gly Phe Asp Val gcc agg gtt cgg Ala Arg Val Arg ctt tat acc tct ttg ggc gat ggc tgg Leu Tyr Thr Ser Leu Giy Asp Gly Trp acg tac Thr Tyr ctt aat tca Leu Asn Ser caa att ccg cag Gin Ile Pro Gin ccg gag cgg gtg Pro Glu Arg Val gcg tcg gga Ala Ser Gly gtg acg tcg Val Thr Ser gtt gcg Val Ala gcg gct ttc cgc acg cat gcg cag att tct Ala Ala Phe Arg Thr His Ala Gin Ile Ser cag ccg att gcg gtg gat Gin Pro Ile Ala Val Asp ttg gag gct gct Leu Giu Ala Ala gag gca gtt gcg Glu Ala Val Ala tcg Ser ttg gcg ggt gtg Leu Ala Gly Val gat Asp 7 5 ccg gac tgt gtt Pro Asp Cys Val ctg ggt ccc acg Leu Gly Pro Thr agg Arg 307 355 403 cag ttt ttg gct Gin Phe Leu Ala aca ttg gcg cgc Thr Leu Ala Arg ttg ggt ggg ttt Leu Gly Gly Phe gta cgt Val1 Arg 100 cga aaa gcg Arg Lys Ala ggc Gly 105 gtg gtg ttg tcg Val Val Leu Ser cgc Arg 110 gcg gac gcg gac Ala Asp Ala Asp tgg ctg acc Trp Leu Thr 115 gag ccc gat Giu Pro Asp gcg ccg ttc cgc tcc ctc gac Ala Pro Phe Arg Ser Leu Asp ggc Gly 125 gtt ttt agc tgg Val Phe Ser Trp ttg ggc Leu Gly 135 acc ggc atg ctg Thr Gly Met Leu ccg Pro 140 gat tgg cag tac Asp Trp Gin Tlyr gag Giu 145 aag ctt gtt gac Lys Leu Val Asp ggc Gly 150 tcg acg cgc ctt Ser Thr Arg Leu gtg ctc agc gcc Val Leu Ser Ala gcg Ala 160 cac ccg ctg ctc His Pro Leu Leu ggc Gly 165 acg gtc gcc cca Thr Val Ala Pro ggc aag att gtg gat aaa gtg cgg gcg Gly Lys Ile Val Asp Lys Val Arg Ala 175 cgt tcg Arg Ser 180 cgt gcc tgg Arg Ala Trp, ctt gtc gac gcc Leu Val Asp Ala acc Thr 190 acc tac gca gcc Thr Tyr Ala Ala tac cgc ccc Tyr Arg Pro 195 WO 01/00843 ctg cgc cta Leu Arg Leu 200 gag ttg ggc Giu Leu Gly 215 atg ttc ccg Met Phe Pro 230 tcc ctg ccg Ser Leu Pro ctg gga aac Leu Gly Asn gag atg gcg Giu Met Ala 280 ctc gaa gga 1027 Leu Glu Gly 295 gca ggt caa 1075 Ala Gly Gin 310 acc atg gaa 1123 Thr Met Glu aat cgt ttg 1171 Asn Arg Leu atg ggt gtg 1219 Met Gly Val 360 ttt agc acc 1267 gac Asp ggc Gly cgc Arg cat Hius c tg Leu 265 aaa Lys ctt Leu gac Asp ggc Gly atc Ile 345 act Thr tac tgg Trp, cag Gin gat Asp 235 c tg Leu gaa Glu cac His gcg Ala ccg Pro 315 ccc Pro acc Thr gct Ala gaa gcc Ala 205 tcg Ser acc Thr ggc Gly gcc Ala gga Gly 285 cat His ctg Leu gat

ASP

agc Ser gga Gly 365 gat gtc Val1 att Ile ctc Leu 240 ccc Pro gtc Vai gag Glu gga Gly gtg Val 320 tac Tyr gac Asp act Thr acc Thr atg Met ttc Phe 225 gaa Giu aac Asn gtt Val1 cat His att Ile 3TO ccc Pro cgc Arg ccg Pro atc Ile agg Arg PCTIIBOOIOO923 ctc ggc 739 Leu Gly acc tcg 787 Thr Ser gca agc 835 Aia Ser 245 cgg cac 883 Arg His 260 atg ggg 931 Met Gly gaa tcg 979 Giu Ser gat gcc Asp Ala acc ttc Thr Phe 325 gtg gac Val Asp 340 gaa gca Giu Ala agc ccg Ser Pro gca tcg Ala Ser Phe Ser Thr Tyr Tyr Giu Val Asp Gin 375 380 ctt gcc taaaccgcaa gcacgagctt gcc 1296 Leu Ala 390 <210> 652 WO 01/00843 WO 0100843PCTIBOOIOO923 <211> 391 <212> PRT <213> Corynebacterium giutainicum <400> 652 Val Gly Phe Asp Val Ala Arg Val Arg Gly Leu Tyr Thr Ser Leu Gly 1 5 10 Asp Gly Trp Thr Tyr Leu Asn Ser His Gin Ile Pro Gin Val Pro Glu 25 Arg Val Ala Ser Giy Val Ala Ala Ala Phe Arg Thr His Ala Gin Ile 40 Ser Giu Val Thr Ser Gin Pro Ile Ala Vai Asp Gin Leu Giu Ala Ala 55 Arg Giu Ala Val Ala Ser Leu Ala Gly Val Asp Pro Asp Cys Val Val 70 75 Leu Gly Pro Thr Arg Gln Phe Leu Ala His Thr Leu Ala Arg Gly Leu 90 Gly Gly Phe Val Arg Arg Lys Ala Gly Val Val Leu Ser Arg Ala Asp 100 105 110 Ala Asp Trp Leu Thr Ala Pro Phe Arg Ser Leu Asp Gly Val Phe Ser 115 120 125 Trp Ala Giu Pro Asp Leu Gly Thr Gly Met Leu Pro Asp Trp, Gin Tyr 130 135 140 Giu Lys Leu Val Asp Gly Ser Thr Arg Leu Val Val Leu Ser Ala Ala 145 150 155 160 His Pro Leu Leu Gly Thr Val Ala Pro Val Gly Lys Ile Val Asp Lys 165 170 175 Val Arg Ala Arg Ser Arg Ala Trp, Val Leu Val Asp Ala Thr Thr Tyr 180 185 190 Ala Ala Tyr Arg Pro Leu Arg Leu Asp Giu Trp Glu Ala Asp Ile Val 195 200 205 Met Leu Asp Leu Gly Glu Leu Gly Gly Pro Gin Ile Ser Ala Leu Ile 210 215 220 Phe Arg Asp Thr Ser Met Phe Pro Arg Leu Asp Arg Thr Val Pro Leu 225 230 235 240 Giu Leu Pro Ala Ser Ser'Leu Pro His Gly Leu Leu Gly Gly Val Pro 245 250 255 Asn Leu Val Arg His Leu Gly Asn Leu Asp Glu Asn Ala Pro Ser Val 260 265 .270 Val Glu Ala Met Gly Giu Met Ala Lys Phe His Lys Gly Leu Phe Giu 275 280 285 His Leu Val Glu Ser Leu Glu Gly Leu His Ala Val His Ile Val Gly 290 295 300 WO 01/00843 WO 0100843PCT/IBOO/00923 Ile Ser Gly Asp Ala Ala Gly Gin Asp Ala Pro 305 310 315 Pro Arg Leu Thr Phe Thr Met Glu Gly Val Pro 325 330 Arg Arg Leu Val Asp Asn Arg Leu Ile Thr Thr 340 345 Pro Leu Leu Giu Ala Met Gly Val Thr Glu Ala 355 360 Ilie Gly Leu Ser Pro Phe Ser Thr Tyr Tyr Glu 370 375 Arg Val Leu Ala Ser Leu Ala 385 390 <210> 653 <211> 638 <212> DNA <213> Corynebacteriun glutamicum Phe Leu Asp Arg Ala Asp Met Val 335 Val Ser Pro Ala 350 Gly Gly Ser Ile 365 Val Asp Gin Leu 380 Val1 320 Tyr Asp Thr Thr <220> <221> CDS <222> <223> FRXA00435 <400> 653 gtc gac go Val Asp A: 1 tgg gaa go Trp, Giu A: cag att to Gin Ilie So gat cgc a4 Asp Arg TI ctg ctc gc Leu Leu G'.

gaa aac go Glu Asn A:' cac aag g His Lys G'.

acc Thr 5 atc Ile t tg Leu cca Pro g tg Val tcc Ser ttt Phe tac Tyr gtc Val1 att Ile ctc Leu ccc Pro 70 gtc Val gag Glu tac Tyr gat Asp gat Asp ccc Pro gtg Val1 gcg Ala gtg Val1 105 cgc ccc Arg Pro 10 ctc ggc Leu Gly acc tcg Thr Ser gca agc Ala Ser cgg cac Arg His 75 atg ggg Met Gly 90 gaa tcg Glu Ser gat gcc Asp Ala cta Leu ggc Gly ccg Pro ccg Pro aac Asn gcg Ala gga Gly 110 caa Gin gac Asp ggc Gly cgc Arg cat His ctg Leu aaa Lys ctt Leu gac Asp gag Giu ccg Pro ctg Leu ggg Gly gat Asp ttc Phe cac His gc Ala gcg gtg cat atc Ala Val His Ilie gtg gga att tcc ggc Val Gly Ile Ser Gly WO 01/00843 WO 0100843PCTTBOO/00923 115 120 ccg ttc ctg gat cga gtg ccc cgc ttg Pro Phe Leu Asp Arg Val Pro Arg Leu 130 135 ccc gca gat atg gtg tac cgc cga ttg Pro Ala Asp Met Val Tyr Arg Arg Leu 145 150 acc gtc agc cct gct gac ccg ctg ctc Thr Vai Ser Pro Ala Asp Pro Leu Leu 165 gct ggc gga tcg atc act atc gga cta Ala Giy Gly Ser Ile Thr Ile Gly Leu 180 185 gaa gtg gat cag ctg acc agg gtg Ctg Glu Val Asp Gin Leu Thr Arg Val Leu 195 200 gcacgagctt gcc <210> 654 <211> 205 <212> PRT <213> Corynebacteriun glutamicum <400> 654 Val Asp Ala Thr Thr Tyr Ala Ala Tyr acc ttc Thr Phe gtg gac Val Asp 155 gaa gca Giu Aia 170 agc ccg Ser Pro gca tcg Ala Ser 125 atg Met cgt Arg ggt Giy agc Ser gcc Ala 205 Arg Leu Phe Leu Gly Met Giu Gly 125 Met Arg gaa ggc gtg Giu Gly Vai ttg atc act Leu Ile Thr 160 gtg act gaa Vai Thr Glu 175 acc tac tat Thr Tyr Tyr 190 taaaccgcaa Giu Ile Arg Leu Asn Lys Val Phe 130 Ala Ala Ser Thr Gly Ala Giy His 115 Leu Asp Val1 Ile Leu Pro Val1 Giu Gly Val1 Tyr 150 Arg Leu Thr Ala Arg Met Glu Asp Thr Val Leu Gly Pro Pro Asn Ala Gly 110 Gin Glu Leu Asp Giy Arg His Leu Lys Leu Asp Giy Ile WO 01/00843 WO 0100843PCTIiBOO/00923 Thr Val Ser Pro Ala Asp Pro Leu Leu Giu 165 170 Ala Met Gly Val Thr Giu 175 Ala Gly Gly Ser Ile Thr Ile Gly Leu 180 185 Giu Val Asp Gin Leu Thr Arg Val Leu 195 200 Ser Pro Phe Ser Thr Tyr Tyr 190 Ala Ser Leu Ala 205 <210> <211> <212> <213> <220> <221> <222> <223> 655 535

DNA

Corynebac terium giutarnicum

CDS

(535) FR.xA02 801 <400> 655 cgacaggtga attcatgcac gtttgagtgt cccgtgtgtg gggtaatgtt gtccaagaga gtggaaggaa atgctgtggc ggttgaaagg agtgcctttc ggt ttt gat Gly Phe Asp gcc agg gtt cgg Ala Arg Val Arg ctt tat acc tct Leu Tyr Thr Ser ggc gat ggc tgg Gly Asp Gly Trp acg tac Thr Tyr ctt aat tca Leu Asn Ser gtt gcg gcg Val Ala Ala caa att ccg cag Gin.Ilie Pro Gin ccg gag cgg gtg Pro Giu Arg Val gcg tcg gga Ala Ser Gly gtg acg tcg Val Thr Ser gct ttc cgc acg Ala Phe Arg Thr gcg cag att tct Ala Gin Ile Ser cag ccg Gin Pro att gcg gtg gat Ilie Ala Vai Asp ttg gag gct gct Leu Giu Ala Ala cgc Arg gag gca gtt gcg Giu Ala Val Ala tcg Ser ttg gcg ggt gtg Leu Ala Gly Val ccg gac tgt gtt Pro Asp Cys Val ctg ggt ccc acg Leu Gly Pro Thr cag ttt ttg gct Gin Phe Leu Ala cat His aca ttg gcg cgc Thr Leu Ala Arg ttg ggt ggg ttt Leu Gly Gly Phe gta cgt ValI Arg 100 cga aaa gcg Arg Lys Ala gcg ccg ttc Ala Pro Phe 120 ggC Gly 105 gtg gtg ttg tcg Val Val Leu Ser gcg gac gcg gac Ala Asp Ala Asp tgg ctg acc Trp Leu Thr 115 gag ccc gat Giu Pro Asp cgc tcc ctc gac Arg Ser Leu ASP gtt ttt agc tgg Val Phe Ser Trp gcc Ala 130 ttg ggc acc ggc atg ctg ccg gat tgg cag tac cag 535 Leu Gly Thr Gly Met Leu Pro Asp Trp Gin Tyr Gin WO 01/00843 PCT/IBO0100923 135 140 145 <210> 656 <211> 145 <212> PRT <213> Corynebacterium giutamicun <400> 656 Val Gly Phe Asp Val Ala Arg Val Arg Gly Leu Tyr Thr Ser Leu Gly 1 5 10 Asp Gly Trp Thr Tyr Leu Asn Ser His Gin Ile Pro Gin Val Pro Giu 25 Arg Val Ala Ser Gly Val Ala Ala Ala Phe Arg Thr His Ala Gin Ile 40 Ser Glu Val Thr Ser Gin Pro Ile Ala Val Asp Gin Leu Giu Ala Ala 55 Arg Glu Ala Val Ala Ser Leu Ala Gly Val Asp Pro Asp Cys Val Val 70 75 Leu Gly Pro Thr Arg Gin Phe Leu Ala His Thr Leu Ala Arg Gly Leu 90 Gly Gly Phe Val Arg Arg Lys Ala Gly Val Val Leu Ser Arg Ala Asp 100 105 110 Ala Asp Trp Leu Thr Ala Pro Phe Arg Ser Leu Asp Gly Val Phe Ser 115 120 125 Trp Ala Giu Pro Asp Leu Gly Thr Gly Met Leu Pro Asp Trp Gin Tyr 130 135 140 Gin 145 <210> 657 <211> 1386 <212> DNA <213> Corynebacterium glutamicun <2 <221> CDS <222> (101)..(1363) <223> RXA02516 <400> 657 ttcattcacg ttttcgcgaa tggccagatt gtgaccaccg gtggcgctga gcttgctgac aagctcgagg ctgacggcta cgaccagttc atcaagtaac atg tcc gat ttc ctc 115 Met Ser Asp Phe Leu 1 aat gca gat gga. tcc ctc aat gtg gat aag gtg cgg gaa gaa ttc cca 163 Asn Ala Asp Gly Ser Leu Asn Val Asp Lys Val Arg Glu Giu Phe Pro 15 WO 01/00843 atc ctg aag Ile Leu Lys tca ggt gcg Ser Gly Ala cac ttt gtg His Phe Val ctg gct gag Leu Ala Glu gct gcc ttt Ala Ala Phe gca act gaa Ala Thr Glu tcc ggt aag Ser Gly Lys 120 cta gag cac Leu Giu His 135 acc ggt gcg Thr Gly Ala 150 gat ctc gat Asp Leu Asp act cac cag Thr His Gin gtt cgt cgt Val Arg Arg 200 cag tct gtt Gin Ser Val 215 ttc tct gca Phe Ser Ala 230 gtt gtg tat Val Val Tyr act Thr tcg Ser cac His gca Ala ggt Gly ctc Leu cgt Arg gca Ala t tg Leu ctc Leu 170 aat Asn aag Lys cat His tct Ser aag Lys 250 gt t Val1 cag Gin acc Thr acg Thr 75 gcc Ala aat Asn gtc Val1 aac Asn aag Lys 155 gag Glu gtg Val1 gct Ala a tg Met ggc G ly 235 tcc Ser gct Ala cgt Arg ggt Gly cgc Arg ttC Phe ggt Gly gtc Val1 130 ctg Leu gat Asp gtc Val1 gtt Val1 ctt Leu 210 ctg Leu gca Ala cca Pro tac Tyr gca Ala gcc Ala gag Glu act Thr gat Asp 115 atc Ile tgc Cys ggt Gly gt t Val1 cca Pro 195 gat Asp gat Asp ggc Gly cca Pro PCTIBOOIOO923 ctg gac 211 Leu Asp gag gag 259 Giu Giu tac caa 307 Tyr Gin aag atc 355 Lys Ile aag aat 403 Lys Asn 100 gac cgt 451 Asp Arg acg gag 499 Thr Glu cgt cga 547 Arg Arg cgc att 595 Arg Ile 165 gcc ttc 643 Ala Phe 180 gag ttg 691 Glu Leu gcg tgc 739 Ala Cys gta gat 787 Val Asp gtg ggc 835 Val Gly 245 ttt ttg 883 Phe Leu 260 act ggt ggt tcc atg att gaa gtt gtc acc atg gag ggt tcc acc tac WO 01/00843 Thr Gly Gly PCT/EBOO/00923 Met Ile Giu Val Thr Met ggc acg Gly Thr gct gcc gca cct Ala Ala Ala Pro 280 caa cgt ttt Gin Arg Phe ggt gct gcc Giy Ala Ala 300 gtt gtg 1027 Vai Val 295 gaa gca 1075 Glu Ala 310 aag ctc 1123 Lys Leu gag cag 1171 Glu Gin cac gat 1219 His Asp ggc cac 1267 Giy His 375 aca gca 1315 Thr Ala 390 cgc ctc 1363 Arg Leu ggc ttg Gly Leu gac atg ctg Asp Met Leu atc gca gcg cat Ilie Ala Ala His 315 acg gca att aag Thr Ala Ilie Lys 330 gag cac gca ttg act Glu His Ala Leu Thr 320 gga cta acc att gct Gly Leu Thr Ile Ala 335 atc agc ttc ggt gtc Ile Ser Phe Gly Val Glu Gly Ser Thr Tyr 275 cag atg acc agc cag Gin Met Thr Ser Gin 290 aat gaa atc ggt atg Asn Giu Ile Gly Met 305 gct tac gcg ttg gaa Ala Tyr Ala Leu Giu 325 ggt cct ttg act gca Gly Pro Leu Thr Ala 340 gag ggc att cac cca Giu Gly Ile His Pro 355 gtg aat atc cgc gtc Val Asn Ile Arg Val 979 cgc ggc Arg Gly 345 ggt gca Gly Ala 350 cta Leu 360 cac His ggc caa gtg ctt gac Gly Gin Val Leu Asp 365 tgc gcg tgg ccc gtg Cys Ala Trp Pro Val gat Asp cac His cag ggc Gin Gly 370 cgc agc atg aac gta caa tcg Ara Ser Met Asn Val Gin Ser aga gca tct ttc Arg Ala Ser Phe 395 ctc Leu 385 tat aac acc ttc Tyr Asn Thr Phe 400 gaa gaa atc gac Giu Giu Ile Asp 405 ttt gga gtt gag Phe Gly Val Giu 420 gcg gca gcg atc gag aag gcc aag Ala Ala Ala Ile Giu Lys Ala Lys 410 415 caa ttc Gin Phe taatgaacct tgagcagatg tac 1386 <210> 658 <211> 421 <212> PRT <213> Corynebacteriun giutamicum <400> 658 Met Ser Asp Phe Leu Asn Ala Asp Gly Ser Leu Asn Val Asp Lys Val 1 5 10 Arg Giu Giu Phe Pro Ilie Leu Lys Arg Thr Val Arg Asp Gly Lys Pro WO 01/00843 WO 0100843PCTflIBOO/00923 Leu Trp Arg Ala Ala Leu Val1 G lu 145 Giu Lys

ASP

Val1 Giu 225 Pro Leu Giu Gin Asn 305 Ala Gly Ala Arg Gly Arg Phe Gly Val 130 Leu Asp Val Val Leu 210 Leu Ala Pro Gly Met 290 Glu Tyr Pro Tyr Ala Al a Glu Thr Asp 115 Ile Cys Gly Val Pro 195

ASP

Asp Gly Pro Ser 275 Thr Ile Ala Leu Ser His Leu 70 Ala Ala Ser Leu Thr 150 Asp Thr Val1 Gin Phe 230 Val1 Thr Ala Val Glu 310 Lys Glu Gly Phe Al a Ala Thr Gly Glu 135 Gly Leu His Arg Ser 215 Ser Val1 G ly Ala Val1 295 Ala Leu Gln Arg Val1 Glu Giu Tyr Asp Trp Va 1 Thr 175 Val1 Leu Phe Leu Asp 255 Thr Gly Met Leu Ile 335 Gly Val His Gly Ile Thr Thr Gin Thr 160 Val Ala Thr His Gly 240 G iu Met Thr Leu Thr 320 Ala Val1 WO 01/00843 WO 0100843PCTIiBOO/00923 Giy Asn 370 Asn Giu Phe Pro His Asp Leu Gly Gin Val Leu Asp Asp 360 365 Val Gly His His Cys Ala Trp, Pro Val His 375 380 Ser Thr Ala Arg Ala Ser Phe Tyr Leu Tyr 390 395 Asp Arg Leu Ala Ala Ala Ile Giu Lys Ala 405 410 Giu Gin Gly Arg Ser Asn Thr 400 Lys Gin 415 <210> 659 <211> 570 <212> DNA <213> Corynebacteriui glutamicun <22 0> <221> CDS <222> (101)..(547) <223> RXA02517 <400> 659 cgacagcaag agcatctttC tatctctata acaccttcga agaaatcgac cgcctcgcgg cagcgatcga gaaggccaag caattctttg gagttgagta atg aac ctt gag cag Met Asn Leu Giu Gin atg Met ggc Gly tgt Cys ac c Thr gcc Ala aag Lys ggt Gly gtg Val1 cct Pro ttg Leu gtC Val1 gtt Val1 aag Lys ggc Gly atc Ile t tc Phe act Thr tcC Ser atg Met 75 ctc Leu gat Asp ctg Leu gat Asp ctg Leu tac Tyr gcc Ala aca Thr gaa Giu gac Asp gct Al a cgc Arg gaa Glu gag Glu gaa Glu gat Asp cac His gag Glu gtg Val1 gca Ala gag Glu ttt Phe ctc Leu 110 tac Tyr 15 gtt Val1 aag Lys gtt Val1 atc Ile gag Glu 95 atc Ile aaa Lys cac His ctg Leu ggt Giy gtg Val1 80 aag Lys gga Gly

I

aac Asn cac His tct Ser tgc Cys 65 ggc Gly atg Met gat Asp cag Gin aac Asn 35 gac Asp atc Ile ccc Pro gtt Val1 gt t Val1 115 cac His 20 cc t Pro ggc Gly agc Ser gtc Val1 tcc Ser 100 gct Ala aag Lys tct Ser tcc Ser cag Gin gac Asp cgc Arg t tc Phe WO 01/00843 WO 0100843PCT/IBOO/00923 tcc gga gtc gcc aag tac ccg gca cgc gtg aag tgc gcg ctg ctt ggg 499 Ser Gly Val Ala Lys Tyr Pro Ala Arg Val Lys Cys Ala Leu Leu Gly 120 125 130 tgg aag gct ttC cag gcg gca acc gct gac gct gtt gcg cac gca cat 547 Trp Lys Ala Phe Gln Ala Ala Thr Ala Asp Ala Val Ala His Ala His 135 140 145 tagcccgctg tattaattgg agg 570 <210> 660 <211> 149 <212> PRT <213>*Corynebacteriun glutamicun <400> 660 Met Asn Leu Glu Gln Met Tyr Gln Glu Val Ilie Leu Asp His Tyr Lys 1 5 10 Asn Pro Gin His Lys Gly Leu Arg Asp Pro Phe Asp Ala Glu Val His 25 His Val Asn Pro Ser Cys Gly Asp Glu Leu Thr Leu Arg Val Lys Leu 40 Ser Glu Asp Gly Ser Thr Val Glu Asp Val Ser Tyr Glu Ala Val Gly 55 Cys Ser Ile Ser Gin Ala Ser Thr Ser Val Met Ala Glu Glu Ile Val 70 75 Gly Gin Pro Val Asp Lys Ala Leu Giu Lys Leu Thr Glu Phe Glu Lys 90 Met Ile Val Ser Arg Gly Gin Phe Val Gly Asp Glu Asp Leu Ile Gly 100 105 110 Asp Gly Val Ala Phe Ser Gly Val Ala Lys Tyr Pro Ala Arg Val Lys 115 120 125 Cys Ala Leu Leu Gly Trp Lys Ala Phe Gin Ala Ala Thr Ala Asp Ala 130 135 140 Val Ala His Ala His 145 <210> 661 <211> 1167 <212> DNA <213> Corynebacteriun glutainicum <220> <221> CDS <222> (101)..(1144) <223> RXA01747 <400> 661 cgaagtagag ccgattgcag aatcggcgga atgagacgtc gaaaagcgtt taagctttcc 931 WO 01/00843 WO 0100843PCTI~IBOOIOO923 ctaaaaatat cactaactcg aaagatgtaa ggttgcattt gtg act atc gca Val Thr Ile Ala gaa gga cga cga Giu Gly Arg Arg cta cgc gtc gaa Leu Arg Val Giu cga aac tca gaa Arg Asn Ser Giu acc ccg Thr Pro att gag acg Ile Giu Thr gag tat cag Giu Tyr Gin aag Lys cct cga tgg att Pro Arg Trp Ile aga Arg 30 aac cag gtc aaa Asn Gin Vai Lys aac gga cct Asn Gly Pro gat atg aag gaa Asp Met Lys Giu gtc gct ggc gca tca cta cac act Vai Ala Giy Aia Ser Leu His Thr gtg tgt Vai Cys cag gag gct ggc Gin Glu Aia Gly cct aat atc cat Pro Asn Ile His tgt tgg gaa tcc Cys Trp, Giu Ser cgt Arg gag gca acc ttc Giu Ala Thr Phe att ggt ggc gcc Ile Giy Giy Ala tgc tct cgc cgc Cys Ser Arg Arg gat ttc tgc atg Asp Phe Cys Met atc Ile aac tcg gct cgc cct gag cca ctc gac Asn Ser Ala Arg Pro Giu Pro Leu Asp 95 cgc ggt Arg Gly 100 gag cca ctg Giu Pro Leu tcc acc atc Ser Thr Ile 120 gtc gct gag tct Val Ala Giu Ser gtt Val1 110 cgt gag atg cag ctg aat tac Arg Glu Met Gin Leu Asn Tyr 115 acc ggt gtt acc Thr Gly Val Thr cgt Arg 125 gat gat ctg gat Asp Asp Leu Asp gaa ggc gca Giu Gly Ala tgg ctg Trp Leu 135 tac tca gaa gtg Tyr Ser Giu Val cgt aag atc cac gag ctg aac cca cac Arg Lys Ile His Giu Leu Asn Pro His 145 ac c Thr 150 ggt gtg gaa aac Gly Val Glu Asn gtg cct gat ttc Val Pro Asp Phe tcC Ser 160 ggc aag aag gat Gly Lys Lys Asp ctg cag gaa gtt Leu Gin Giu Val gaa tcc cgc cca Giu Ser Arg Pro gtt ttc gct cac Val Phe Ala His aac gtg Asn Val 180 gaa act gtg Giu Thr Vai gag cgt tca Giu Arg Ser 200 cca Pro 185 cgt att ttc aag Arg Ile Phe LYS cgc Arg 190 att cgc cca gca Ile Arg Pro Ala ttc cgc tac Phe Arg Tyr 195 ggt ctg gtg Gly Leu Vai ctt gat gtg atc Leu Asp Val Ile cg t Arg 205 cag gct cgc gat Gin Aia Arg Asp t tc Phe 210 acc aag Thr Lys 215 tcc aac ctg att Ser Asn Leu Ile ggc atg ggt gaa Giy Met Gly Giu acc Thr 225 aag gaa gaa atc Lys Giu Giu Ile WO 01/00843 PCTIIBOO/00923 tc acc 835 acc Thr 230 a tc Ile tgg gtg Trp Val atg ggc Met Gly cgt gca 1027 Arg Al a 295 atc cca 1075 Ile Pro 310 gcc cag 1123 Ala Gin acc cca 1167 Thr Pro ctg cag gat Leu Gin Asp 235 tac ctg cgt Tyr Leu Arg 250 cct gag gag Pro Giu Giu 265 gct gct gtt Ala Ala Val cgt ctg tac Arg Leu Tyr cac ctc gcg His Leu Ala 315 gca tct aca Ala Ser Thr 330 gtg tcc ttc Val Ser Phe 345 gac Asp ccc Pro gc t Ala cgt Arg 290 cgt Arg gga Gly gct atc Ile atc Ile gca Aia 275 tcc Ser ggc Gly gga Gly t cc gcg cag gcc acg Ala Gin Ala Met 300 cac ctg aag gat His Leu Lys Asp ctt ctg gag cgt Leu Leu Giu Arg 335 Tyr Gly Ala Ser Glu Asp aac Asn taagcccgaa gttttttaac cgc 662 <211> 348 <212> PRT <213> Corynebacterium giutarnicum <400> 662 Val Thr Ile Ala Pro Glu Gly Arg Arg Leu 1 5 Asn Ser Giu Thr Pro Ile Glu Thr Lys Pro 25 Val Lys Asn Gly Pro Glu Tyr Gin Asp Met Ala Ser Leu His Thr Val Cys Gin Giu Ala Glu Cys Trp Glu Ser Arg Glu Aia Thr'Phe Cys Ser Arg Arg Cys Asp Phe Cys Met Ile Giu Arg Val1 Asn Gly Arg WO 01/00843 PCT/IB00/00923 Pro Leu Asp Arg Gly Glu Pro Leu Arg Val Ala Glu Ser Val Arg Glu 100 105 110 Met Gin Leu Asn Tyr Ser Thr Ile Thr Gly Val Thr Arg Asp Asp Leu 115 120 125 Asp Asp Glu Gly Ala Trp Leu Tyr Ser Glu Val Val Arg Lys Ile His 130 135 140 Glu Leu Asn Pro His Thr Gly Val Glu Asn Leu Val Pro Asp Phe Ser 145 150 155 160 Gly Lys Lys Asp Leu Leu Gin Glu Val Phe Glu Ser Arg Pro Glu Val 165 170 175 Phe Ala His Asn Val Glu Thr Val Pro Arg Ile Phe Lys Arg Ile Arg 180 185 190 Pro Ala Phe Arg Tyr Glu Arg Ser Leu Asp Val Ile Arg Gin Ala Arg 195 200 205 Asp Phe Gly Leu Val Thr Lys Ser Asn Leu Ile Leu Gly Met Gly Glu 210 215 220 Thr Lys Glu Glu Ile Thr Glu Ala Leu Gin Asp Leu His Asp Ala Gly 225 230 235 240 Cys Asp Ile Ile Thr Ile Thr Gin Tyr Leu Arg Pro Gly Pro Leu Phe 245 250 255 His Pro Ile Glu Arg Trp Val Lys Pro Glu Glu Phe Leu Glu His Ala 260 265 270 Asp Ala Ala Lys Glu Met Gly Phe Ala Ala Val Met Ser Gly Pro Leu 275 280 285 Val Arg Ser Ser Tyr Arg Ala Gly Arg Leu Tyr Ala Gin Ala Met Glu 290 295 300 Phe Arg Gly Glu Glu Ile Pro Ala His Leu Ala His Leu Lys Asp Thr 305 310 315 320 Ser Gly Gly Ser Thr Ala Gin Glu Ala Ser Thr Leu Leu Glu Arg Tyr 325 330 335 Gly Ala Ser Glu Asp Thr Pro Val Val Ser Phe Asn 340 345 <210> 663 <211> 876 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(853) <223> RXA01746 <400> 663 aaaccgccac tcccctttca ctggggagtg gcggttttgt cgtttcatgc atgcagtgtg 934 WO 01/00843 WO 0100843PCT/IiBOOI00923 tgacttatca accttgttag ggctagggtg gatatctatc atg act gca cca Met Thr Ala Pro gat cct ttt ttc Asp Pro Phe Phe ccc Pro gca gat ctt tct Ala Asp Leu Ser cgc gcg tct gca Arg Ala Ser Ala gag ccc Glu Pro att gaa att Ile Glu Ile tat caa gca Tyr Gin Ala cgg ttg ggt ttg Arg Leu Gly Leu gat tat caa gag Asp Tyr Gin Giu gcc tgg gat Ala Trp Asp atc cct gat Ile Pro Asp gag ctt gct acc Giu Leu Ala Thr agg gct aat gat Arg Ala Asn Asp cag ctg Gin Leu ctt att ttg gag Leu Ilie Leu Giu ccg tcg gtg tat acc gca ggt aag cgc Pro Ser Val Tyr Thr Ala Gly Lys Arg cag ccg gaa gat Gin Pro Glu Asp ccc acc aac gga Pro Thr Asn Gly ctg Leu ccg gtg atc aat Pro Val Ile Asn gat cgt ggt ggt Asp Arg Giy Giy cgc Arg atc acg tgg cat Ile Thr Trp, His ggt Giy 95 cct ggc caa ttg Pro Gly Gin Leu gtg atc Val Ile 100 tat ccg atc Tyr Pro Ilie aga cgc ctc Arg Arg Leu 120' atc Ile 105 aaa tta gcc gat Lys Leu Ala Asp atc gat gtg gtt Ile Asp Val Val gat tac gta Asp Tyr Val 115 atg ggt gtt Met Gly Val gag gaa gcg ctc Glu Giu Ala Leu caa gtt gtc ggc Gin Val Val Gly gcc ggc Aia Giy 135 gct ggg cgc att Ala Gly Arg Ilie ggg cgt tcg ggt Gly Arg Ser Gly gtg Vai 145 tgg gtg cca gct Trp Val Pro Ala gat ggt tgg gtg Asp Gly Trp Val agc aag gtt gcg Ser Lys Val Ala gcc Ala 160 atc ggc att cga Ile Gly Ile Arg act cgt ggt gtt Thr Arg Gly Val gca.

Ala 170 atg cac ggt gtg Met His Gly Vai atc aac tgc aac Ile Asn Cys Asn aac acg Asn Thr 180 ttg gat ttc Leu Asp Phe ttg agc aca Leu Ser Thr 200 tat Tyr 185 gag cac atc att Glu His Ile Ile tgt ggc att gct Cys Giy Ile Ala gat gca ggc Asp Aia Gly 195 gtt gag gaa.

Val Giu Glu ctc tcg agg gaa Leu Ser Arg Glu ctg Leu 205 aaa agg gac gtt Lys Arg Asp Val tta gtc Leu Val 215 gag cca tcg atc Glu Pro Ser Ile cgc Arg 220 gca ttg gat gat gct ttg gct ggt cgg Ala Leu Asp Asp Aia Leu Ala Gly Arg 225 WO 01/00843 WO 0100843PCT/IBOO/00923 ctg gtt gtt tct gat cat tct ttc ggc agc gcg ccc gac cca act aag 835 Leu Val Val Ser Asp His Ser Phe Gly Ser Ala Pro Asp Pro Thr Lys 230 235 240 245 aat ctc cct aaa cgg ggg tagtacgagg aattttgtcg gtg 876 Asn Leu Pro Lys Arg Gly 250 <210> 664 <211> 251 <212> PRT <213> Corynebacterium glutamicun <400> 664 Met Thr Ala Pro Asp Pro Phe Phe Ser Glu Glu Ala Val1 Gin Val Asp 130 Trp Gly Cys Ala Ser 210 Leu Al a Ala Ile Gly Ile Leu Asp 115 Met Val1 Ile Asn Asp 195 Val1 Ala Pro Arg Leu Leu Asp Arg Lys Glu Arg Val1 Al a 170 Glu Ser Ser Asp Ala Leu Ala Glu Leu Ile Leu Ala Ile Asp 155 Met His Arg Ile His 235 Leu Leu Arg Pro Thr Trp Asp Ile 125 Gly Lys Gly Ile Leu 205 Al a Phe Arg Tyr Asn Tyr Leu Pro Asp Val Gly Ala 160 Ile Gly Asp Asp Ala 240 Pro Asp Pro Thr Lys Asn Leu Pro Lys Arg Gly WO 01/00843 WO 0100843PCT/IBO0100923 245 <210> 665 <211> 1179 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1156) <223> RXA02106 <400> 665 ggtcgccgct tttcgacgcc cgcctgcggc gggatgactg tgatggaggg gcgcgtcgac aagcaaaatc tctttagcaa attcggttac tgtggggcgc atg aat aac cat ttt Met Asn Asn His Phe gag Giu gat Asp gaa Glu tcg Ser ttg Leu att Ile gat Asp aac Asn cgt Arg atc Ile 150 gga Gly gac Asp t tc Phe gat

ASP

gtt Val Cgg Arg c tg Leu C tC Leu att Ile 140 tat Tyr aag Lys gtg Val1 gcc Ala cgt Arg gag Glu gca Ala cac His ctt Leu 125 tgg Trp gtc Val Ct t Leu aag Lys ctt Leu ttg Leu cta Leu gtc Val cca Pro 110 ttg Leu gat Asp aat Asn gtc Val at t Ile Gly cag Gin cac His acc Thr ggg Gly gac Asp tgg Trp gaa Glu gtc Vai tcc Ser cgg Arg gca Ala 9gC Gly 80 ggc Gly gtg Val caa Gin gat Asp atc Ile 160 1 gtt Val1 Giy gcg Aia aag Lys ttt Phe gcg Ala ctt Leu gt t Val1 gat Asp 145 aac Asn gat Asp gat Asp ctg Leu ttg Leu agc Ser caa Gin c~t Pro gcc Ala 130 cgc Arg caa Gin acc Thr t tC Phe ggg G ly g~a Ala gcg Ala t tc Phe 100 cca Pro ggt Gly aca Thr ggc Gly acc Thr ctc Leu gcg Ala gcg Ala gat Asp acc Thr ctt Leu cag Gin gtg Val gtt Val 165 163 211 259 307 355 403 451 499 547 595 643 aat gaa cat ggt gtg acc gtg gta cga cgc atg tct ggt ggc ggt gca WO 01/00843 Asn Giu His PCT/iBOO/00923 Gly Ala 180 Gly Val Thr Val Val Arg Arg met Ser Gly Gly 170 175 atg ttt Met Phe gaa tct Glu Ser gat cgt Asp Arg 215 tac gtg Tyr Val 230 gct gca Ala Ala tcc tat Ser Tyr aag gtg Lys Val gat cct 1027 Asp Pro 295 ace eta 1075 Thr Leu 310 cte age 1123 Leu Ser tac gee 1176 atg Met ctc Leu 200 tgg Trp cc t Pro cag Gin gac Asp aag Lys 280 ctg Leu aag Lys gat Asp acc gag Glu 185 gt t Val1 gtg Val1 ate Ile aaa Lys ate Ile 265 att Ilie ege Arg tee Ser gaa Glu gag gge Gly get Ala att Ile aat Asn cgt Arg 250 gat Asp tee Ser ege Arg aea Thr gat Asp 330 gag tge Cys age Ser 205 ctg Leu ac Thr gge Gly atg Met ggt Giy 285 ggt Gly get Ala gea Ala aag tat Tyr eag Gin eac Hius gge Gly 240 ete Leu eag Gin age Ser egt Arg gge Gly 320 gae Asp tee S er tee Ser gat Asp 225 gga Gly eac His gtg Val gea Ala gag Glu 305 geg Ala eta Leu etg Leu tat Tyr 210 gtt Val1 aa a Lys cae His t tg Leu aag Lys 290 e aa Gin eaa Gin gta Val1 gtt eaa tagtttetat ggatctgeae Tyr Ala Thr Giu Glu Trp Thr Lys Arg Val Gin aag 1179 <210> 666 <211> 352 <212> PRT <213> Corynebaeterium glutaicjum <400> 666 WO 01/00843 WO 0100843PCT/EBOO/00923 Met Asn Asn His Phe Giu Leu Lys Val Pro Gly Gly Lys Leu 1 5 10 Val Val Val1 Giy Ala Lys Phe Ala Leu Val Asp 145 Asn Ser Ser Ser Asp 225 Gly His Val1 Ala Giu 305 Asp Asp Leu Leu Ser Gin Pro Ala 130 Arg Gin Gly Leu Tyr 210 Val Lys His Leu Lys 290 Gin Val Phe Gin Asp Thr Asp Thr 115 Ser Ala Giu Gly Tyr 195 Giu Asp Ile Val Arg 275 Lys Ile Thr Phe Gly Ala Ala Phe 100 Pro Gly Thr Gly Gly 180 Ala Tyr Ala Gly Thr 260 Ile Arg Ile Asp Giu Ser Leu 70 Ile Asp Asn Arg Ile 150 Asn Met Glu Asp Tyr 230 Ala Ser Lys Asp Thr 310 Ser Ile Glu Ala Asp Asn Tyr Asp Ala Val Trp, Glu 105 Leu Asp Thr Met Phe Gin Gly Val 170 Giu Gly 185 Vai Ala Val Ile Ile Asn Lys Arg 250 Ile Asp 265 Ile Ser Arg Arg Ser Thr Ala Phe Thr Asp Arg Ile Glu Arg Ser 155 Thr Gly Gly Ala Asp 235 Arg Ala Asp Gln Phe 315 Asp Phe Asp Val Arg Leu Leu Ile 140 Tyr Val1 Asn Leu Ala 220 Ile Ser Asp Lys Thr 300 Ser Val1 Ala Arg Glu Ala His Leu 125 Trp Val Val Cys Ser 205 Leu Thr Gly Met Gly 285 G ly Ala Ala Gin Glu Val Giu Leu Ser Asp Giu Asp Phe Ala Ala Gly His Asp WO 01/00843 WO 0100843PCTIBOO/00923 Leu Val Lys Thr Lys Tyr Ala Thr Giu Giu Trp Thr Lys Arg Val Gin 340 345 350 <210> 667 <211> 403 <212> DNA <213> Corynebacteriun glutanicun <220> <221> <222> <223>

CDS

(101)..(403) R.XS 01183 <400> 667 cttgatatga cccgaacacc acacatcaca aattgaatcg gtatcctttg gggtattagt ttccgtttta acgacacgac ttgcgaggag tcttaaaata atg Met 1 gcg ttc tcc Ala Phe Ser gag atg ccc gag Giu Met Pro Glu ggc gaa tca gta Gly Giu Ser Val gaa ggc acg atc Giu Gly Thr Ile acc cag Thr Gin tgg ttg aag Trp Leu Lys gag gtc tca Giu Val Ser tc t Ser gtt ggt gac act Val Giy Asp Thr gag gta gat gag ccg ttg ctc Giu Vai Asp Glu Pro Leu Leu act gac aag gtc Thr Asp Lys Vai gac Asp 45 acc gag att ccc Thr Giu Ile Pro tct Ser cct gtC gcc Pro Val Ala ggt gtc Gly Vai atc cta gag att Ile Leu Giu Ile gct gaa gag gat Ala Giu Giu Asp acc gtc gac gtC Thr Val Asp Val ggc Giy ggt gtc att gca Gly Val Ile Ala atc ggc gat gct Ilie Giy Asp Ala gag act cct gcc Glu Thr Pro Ala gaa gct cct gcc Giu Ala Pro Ala gag gca cca gct Giu Ala Pro Ala gcc gaa gag gaa Ala Giu Giu Glu gaa. cca Giu Pro 100 <210> 668 <211> 101 <212> PRT <213> Corynebacteriun giutamicum <400> 668 Met Ala Phe Ser Val Glu Met Pro Giu Gly Giu Ser Val Thr Giu Gly Thr Ilie Thr Gin Trp, Leu Lys Ser Val Gly Asp Thr Val Giu Vai WO 01/00843 WO 0100843PCTIIBOO/00923 Asp Glu Pro Ser Asp Thr Glu Thr Glu Glu Pro Pro Val1 Pro Giu Leu Glu Val Ser Thr Asp Lys Val Asp Thr 40 Ala Gly Val Ile Leu Glu Ile Lys Ala Glu 55 Val Gly Gly Val Ile Ala Ile Ile Gly Asp 70 Asn Glu Ala Pro Ala Asp Glu Ala Pro Ala Pro <210> 669 <211> 1305 <212> DNA <213> Corynebacterium glutamicum <220>' <221> CDS <222> (101) (1282) <223> RXS01260 <400> 669 ctaaacgtgg gctgcattcc ttccaaagtc tctgatcaaa ctttacccat gagaagaaga ccttcggcat caatggcgaa aacgctgaag ttgcccatac gtg Val acc ttc aac tat Thr Phe Asn Tyr gag gat gct Glu Asp Ala ggc gtt cat Gly Val His ctt gga Leu Gly gat gct Asp Ala tcg gta Ser Vai tct ttt Ser Phe att gtt Ile Val aag Lys t tg Leu aag Lys acc Thr acc Thr cag Gin ggc tcc Ser aag Lys gc t Ala ac c Thr 60 cgt Arg ctt Leu att cgt Arg aag Lys aag Lys ttt Phe ggc Gly aac Asn gga ggc gtt Gly Val '15 aac aag Asn Lys act ctt Thr Leu gat gac Asp Asp gtt gac Val Asp cct gtt Pro Val 95 atg gaa 1 c aag atc gtt p LYS Ile Val 20 c gaa att cat e Glu Ile His 35 c acc gac ggt 1 Thr Asp Gly 50 c atc gca acc e Ile Ala Thr 5 a gag aac gtt r Glu Asn Val a aag aag atg o Lys Lys Met 100 gga Gly ggt Gly aag Lys ggt Gly gtg Val1 gtc Val1 ggt Gly 115 163, 211 259 307 355 403 451 ttc gcc tac gtt ctt Giy Ala Gly Ala Ile Gly Met Glu Phe Ala Tyr Val Leu WO 01/00843 WO 0100843PCTIBOO/00923 105 110 115 aac tac ggt gta gat gta acc gtc atc gag ttc atg gat cgt gtg ctt 499 Asn Tyr Gly Val Asp Val Thr Val Ile Giu Phe Met Asp Arg Val Leu 120 125 130 cca aat gaa gat gct gaa gtc tcc aag gtt att gca aag gcc tac aag 547 Pro Asn Giu Asp Ala Giu Val Ser Lys Val Ile Ala Lys Ala Tyr Lys 135 140 145 aag atg ggc gtt aag ctt ctt cct ggc cat gca acc act gct gtt cgg 595 Lys Met Gly Val Lys Leu Leu Pro Gly His Ala Thr Thr Ala Val Arg 150 155 160 165 gac aac ggt gac ttt gtc gag gtt gat tac cag aag aag ggc tct gac 643 Asp Asn Gly Asp Phe Val Giu Val Asp Tyr Gin Lys Lys Gly Ser Asp 170 175 180 aag aca gag act ctt act gtt gat cga gtc atg gtt tcc gtt ggt ttc 691 Lys Thr Giu Thr Leu Thr Val Asp Arg Val Met Val Ser Val Gly Phe 185 190 195 cgt cca cgc gtt gag gga ttt ggt ctt gaa aac act ggc gtt aag ctc 739 Arg Pro Arg Val Giu Gly Phe Gly Leu Giu Asn Thr Gly Val Lys Leu 200 205 210 acc gag cgt ggc gca atc gag atc gat gat tac atg cgt acc aac gtc 787 Thr Glu Arg Gly Ala Ile Giu Ile Asp Asp Tyr Met Arg Thr Asn Val 215 220 225 gat ggc att tac gcc atc ggt gac gtg acc gcc aag ctt cag ctt gct 835 Asp Gly Ilie Tyr Ala Ile Giy Asp Val Thr Ala Lys Leu Gin Leu Ala 230 235 240 245 cac gtc gca gaa gca cag ggc att gtt gcc gca gag act att gct ggt 883 His Vai Ala Giu Ala Gin Gly Ile Val Ala Ala Glu Thr Ile Ala Gly 250 255 260 gca gaa act cag act ctt ggt gat tac atg atg atg cca cgt gca acc 931 Ala Giu Thr Gin Thr Leu Gly Asp Tyr Met Met Met Pro Arg Ala Thr 265 270 275 ttc tgc aac cca cag gtt tct tcc ttt ggt tac acc gaa gag cag gcc 979 Phe Cys Asn Pro Gin Val Ser Ser Phe Gly Tyr Thr Giu Giu Gin Ala 280 285 290 aag gag aag tgg cca gat cgt gag atc aag gtt gct tcc ttc cca ttc 1027 Lys Giu Lys Trp Pro Asp Arg Giu Ile Lys Vai Ala Ser Phe Pro Phe 295 300 305 tct gca aac ggt aaa gca gtt ggc ctg gca gaa act gat ggt ttc gca 1075 Ser Ala Asn Gly Lys Ala Val Gly Leu Ala Giu "Thr Asp Gly Phe Ala 310 315 320 325 aag atc gtt gct gat gca gaa ttc ggt gag ctg ctc ggt gca cac ctg 1123 Lys Ile Val Ala Asp Ala Giu Phe Gly Giu Leu Leu Gly Ala His Leu 330 335 340 WO 01/00843 WO 0100843PCTIBOOIOO923 gtt gga gca aat gca tca gag ctc atc aat gaa ttg gtg ctt gct cag 1171 Val Gly Ala Asn Ala Ser Giu Leu Ile Asn Giu Leu Val Leu Ala Gin 345 350 355 aac tgg gat ctc acc act gaa gag atc tct cgt agc gtc cat att cac 1219 Asn Trp, Asp Leu Thr Thr Glu Glu Ile Ser Arg Ser Val His Ile His 360 365 370 cca acg cta tct gag gca gtt aag gaa gct gca cac ggt atc tct gga 1267 Pro Thr Leu Ser Glu Ala Val Lys Giu Ala Ala His Gly Ilie Ser Gly 375 380 385 cac atg atc aac ttc tagaatccac ctcgttggcc ctg 1305 His Met Ile Asn Phe 390 <210> 670 <211> 394 <212> PRT <213> Corynebacteriun glutainicum <400> 670 Val Thr Phe Asn Tyr Glu Asp Ala His Lys Arg Ser Arg Gly Val Ser 1 5 10 Asp Lys Ile Val Gly Gly Val His Tyr Leu Met Lys Lys Asn Lys Ilie 25 Ile Glu Ilie His Giy Leu Gly Asn Phe Lys Asp Ala Lys Thr Leu Glu 40 Val Thr Asp Gly Lys Asp Ala Gly Lys Thr Ile Thr Phe Asp Asp Cys 55 Ilie Ile Ala Thr Gly Ser Val Val Asn Thr Leu Arg Gly Vai Asp Phe 70 75 Ser Giu Asn Val Val Ser Phe Glu Giu Gin Ile Leu Asn Pro Vai Ala 90 Pro Lys Lys Met Val Ile Vai Gly Ala.Gly Ala Ile Gly Met Giu Phe 100 105 110 Ala Tyr Val Leu Gly Asn Tyr Gly Val Asp Val Thr Val Ile Giu Phe 115 120 125 met Asp Arg Val Leu Pro Asn Glu Asp Ala Giu Vai Ser Lys Val Ile 130 135 140 Ala Lys Ala Tyr Lys Lys Met Gly Val Lys Leu Leu Pro Giy His Ala 145 150 155 160 Thr Thr Ala Val Arg Asp Asn Gly Asp Phe Vai Giu Val Asp Tyr Gin 165 170 175 Lys Lys Gly Ser Asp Lys Thr Glu Thr Leu Thr Vai Asp Arg Val Met 943 WO 01/00843 WO 0100843PCTJLBOO/00923 180 185 190 Val Ser Val Gly Phe Arg Pro Arg Val Glu Gly Phe Gly Leu Glu Asn 195 200 205 Thr Gly Val Lys Leu Thr Giu Arg Gly Ala Ile Giu Ile Asp Asp Tyr 210 215 220 Met Arg Thr Asn Val Asp Giy Ile Tyr Ala Ile Giy Asp Vai Thr*Ala 225 230 235 240 Lys Leu Gin Leu Ala His Val Ala Giu Ala Gin Gly Ile Val Ala Ala 245 250 255 Giu Thr Ile Ala Gly Ala Glu Thr Gin Thr Leu Gly Asp Tyr Met Met 260 265 270 Met Pro Arg Ala Thr Phe Cys Asn Pro Gin Val Ser Ser Phe Gly Tyr 275 280 285 Thr Glu Glu Gin Ala Lys Giu Lys Trp Pro Asp Arg Giu Ile Lys Val 290 295 300 Ala Ser Phe Pro Phe Ser Ala Asn*Gly Lys Ala Vai Gly Leu Ala Glu 305 310 315 320 Thr Asp Giy Phe Ala Lys Ile Val Ala Asp Ala Giu Phe Giy Giu Leu 325 330 335 Leu Gly Ala His Leu.Vai Gly Ala Asn Ala Ser Giu Leu Ile Asn Giu 340 345 350 Leu Vai Leu Ala Gin Asn Trp Asp Leu Thr Thr Giu Giu Ile Ser Arg 355 360 365 Ser Vai His Ile His Pro Thr Leu Ser Glu Ala Val Lys Giu Ala Aia 370 375 380 His Giy Ilie Ser Gly His Met Ile Asn Phe 385 390 <210> 671 <211> 294 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(271) <223> RXS01261 <400> 671 gtgggtgttt ttcattttct tccactctaa aattaagtat ggaaaaccaa ccgcacccgg atgcacgaca atgacccact aaacacgtat ccttgaatgc gtg act gaa cat tat 115 Val Thr Giu His Tyr 1 gac gta gta gta ctc gga gcc ggc ccc ggt ggc tat gtc tcc gcc atc 163 Asp Val Val Val Leu Gly Ala Gly Pro Gly Gly Tyr Val Ser Ala Ile 944 WO 01/00843 WO 0100843PCTI/iBOO/00923 cgt gca gcg cag ctt ggc aag aag gtt gct gta att gag aag cag tac Arg Ala Ala Gin Leu Gly Lys Lys Val Ala Val Ile Glu Lys Gin Tyr 30 tgg ggt ggt Trp, Giy Giy gtt tgc cta aac gtg ggc tgc att cct Val Cys Leu Asn Vai Giy Cys Ile Pro aaa gtc tct Lys Val Ser gat caa aaa cgc Asp Gin Lys Arg tgaagttgcc cataccttta ccc <210> 672 <211> 57 <212> PRT <213> Corynebacterium giutamicun <400> 672 Vai Thr Giu His Tyr 1 5 Asp Vai Vai Vai Giy Ala Giy Pro Giy Gly Tyr Vai Ser Ilie Giu Lys Pro Ser Lys Ilie Arg Ala Ala Leu Giy Lys Lys Vai Aia Vai Gly Cys Ile Gin Tyr Trp Giy Val Cys Leu Asn Vai Ser Asp Gin Lys Arg <210> 673 <21i> 1005 <212> DNA <213> Corynebacteriun <220> <22i> CDS <222> (101)..(982) <223> RXA02717 <400> 673 aggcatgtcc ctaacgaaca giutamicum tcccagcctc atctcaatgg gcaattagcg acgttttgaa gcgtccttca cccggccgag tacctttttc tgtcgagttt gat gca gct gaa gag cgt ctt tac cgc gca gca Asp Ala Ala Giu Giu Arg Leu Tyr Arg Ala Ala 15 atg cca ccc cgc gac Met Pro Pro Arg Asp 1 gag gtc ttc cat gac Giu Vai Phe His Asp gct ggc gga tca acc Ala Gly Gly Ser Thr ctc ggt gca tcg ttt gtc tcc gtg Leu Gly Ala Ser Phe Val Ser Val act tat ggt Thr Tyr Giy 30 cgt gag aga acc tca cgt att gct cga cga tta gcg aaa caa ccg ttg Arg Giu Arg Thr Ser Arg Ile Ala Arg Arg Leu Ala Lys Gin Pro Leu WO 01/00843 WO 0100843PCTIiBOO/00923 acc act Thr Thr ctg gtg cac ctg Leu Val His Leu acc Thr ctg gtt aac cac Leu Val Asn His cgc gaa gag atg Arg Glu Glu Met gca att ctt cgg Ala Ilie Leu Arg tac cta gag ctg Tyr Leu Giu Leu tta aca aac ctg Leu Thr Asn Leu gcg ctt cga gga Ala Leu Arg Gly gat Asp ccg cct gga gac Pro Pro Gly Asp tta ggc gat tgg Leu Gly Asp Trp gtg agc Val Ser 100 acc gat gga Thr Asp Gly tcc act cct Ser Thr Pro 120 ctg aac tat gcc Leu Asn Tyr Ala tc t Ser 110 gag ctc atc gat Giu Leu Ile Asp ctt att aag Leu Ile Lys 115 tcc ttc ccc Ser Phe Pro gag ttc cgg gaa Giu Phe Arg Giu t tc Phe 125 gac ctc ggt atc Asp Leu Gly Ile gaa ggg Giu Gly 135 cat ttc cgg gcg His Phe Arg Ala aaa Lys 140 act cta gaa gaa Thr Leu Giu Giu acc aaa tac act Thr Lys Tryr Thr ctg Leu 150 gcg aag ctg cgt Ala Lys Leu Arg gga Gly 155 ggg gca gag tac Gly Ala Giu Tyr atc acg cag atg Ile Thr Gin Met ttt gat gtg gaa Phe Asp Vai Giu gac Asp 170 tac ctg cga ctt Tyr Leu Arg Leu gat cgc ctt gtc Asp Arg Leu Val gct gca Ala Ala 180 gac ccc att Asp Pro Ilie acg agc ctg Thr Ser Leu 200 ggt gcg aag cca atc att cct ggc atc Gly Ala Lys Pro Ilie Ile Pro Gly Ile 190 atg ccc att Met Pro Ile 195 ggt gct caa Gly Aia Gin cgg tct gtg cgt Arg Ser Val Arg cga Arg 205 cag gtc gaa ctc Gin Val Giu Leu tc t Ser 210 ttg ccg Leu Pro 215 agc caa cta gaa Ser Gin Leu Glu gaa Giu 220 tca ctt gtt cga Ser Leu Val Arg gca aac ggc aat Ala Asn Gly Asn gaa Giu 230 gaa gcg aac aaa Giu Ala Asn Lys gac Asp 235 gag atc cgc aag Giu Ile Arg Lys ggc att gaa tat Gly Ile Glu Tyr tcc Ser 245 acc aat atg gca Thr Asn Met Ala gag Giu 250 cga ctc att gcc Arg Leu Ile Ala gaa Glu 255 ggt gcg gaa gat Gly Ala Giu Asp ctg cac Leu His 260 ttc atg acg Phe Met Thr ctt ggc atg Leu Gly Met 280 aac ttc acc cgt Asn Phe Thr Arg gca Ala 270 acc caa gaa gtg Thr Gin Giu Val ttg tac aac Leu Tyr Asn 275 gac gcg gtg Asp Ala Val gcg cct gct tgg Ala Pro Ala Trp gga Gly 285 gca gag cac ggc Ala Giu His Gly WO 01/00843 WO 0100843PCTIBOO/00923 cgt taagccctct taggaatcat gaa 1005 Arg <210> 674 <211> 294 <212> PRT <213> Corynebacteriun giutamicun <400> 674 Met Pro Pro Arg Asp Asp Ala Ala Glu Glu Arg Leu Tyr Arg Ala Ala 1 5 10 Glu Val Phe His Asp Leu Gly Ala Ser Phe Val Ser Val Thr Tyr Gly 25 Ala Gly Gly Ser Thr Arg Glu Arg Thr Ser Arg Ile Ala Arg Arg Leu 40 Ala Lys Gin Pro Leu Thr Thr Leu Vai His Leu Thr Leu Val Asn His 55 Thr Arg Giu Giu Met Lys Ala Ile Leu Arg Glu Tyr Leu Giu Leu Gly 70 75 Leu Thr Asn Leu Leu Ala Leu Arg Giy Asp Pro Pro Giy Asp Pro Leu 90 Gly Asp Trp Val Ser Thr Asp Gly Gly Leu Asn Tyr Ala Ser Giu Leu l00 105 110 Ilie Asp Leu Ilie Lys Ser Thr Pro Giu Phe Arg Giu Phe Asp Leu Gly 115 120 125 Ile Ala Ser Phe Pro Glu Gly His Phe Arg Ala Lys Thr Leu Giu Giu 130 135 140 Asp Thr Lys Tyr Thr Leu Ala Lys Leu Arg Gly Gly Ala Giu Tyr Ser 145 150 155 160 Ile Thr Gin Met Phe Phe Asp Val Giu Asp Tyr Leu Arg Leu Arg Asp 165 170 175 Arg Leu Val Ala Ala Asp Pro Ile His Gly Ala Lys Pro Ile Ile Pro 180 185 190 Gly Ile Met Pro Ile Thr Ser Leu Arg Ser Val Arg Arg Gln Val Glu 195 200 205 Leu Ser Gly Ala Gin Leu Pro Ser Gin Leu Giu Giu Ser Leu Val Arg 210 215 220 Ala Ala Asn Gly Asn Giu Giu Ala Asn Lys Asp Glu Ile Arg Lys Val 225 230 235 240 Gly Ile Glu Tyr Ser Thr Asn met Ala Giu Arg Leu Ile Ala Giu Gly 245 250 255 WO 01/00843 WO 0100843PCT/EBOO/00923 Ala Glu Asp Leu His Phe Met Thr Leu Asn Phe Thr Arg Ala Thr Gin 260 265 270 Giu Val Leu Tyr Asn Leu Gly Met Ala Pro Ala Trp Gly 275 280 285 Ala Giu His Gly Gin Asp Ala Val Arg 290 <210> 675 <211> 601 <212> DNA <213> Corynebacterium glutamicum <220> <221> <222> <223>

CDS

(101)..(601) RXN0 2027 <400> 675 tcacgtgctc atcgataggc aaactcatgg aaccaagagt acctgccccc gcaaccccaa tcgtcgtacc cctccgaaaa taacggttat ccttagattt atg agc caa act aag Met Ser Gin Thr Lys cag gaa ctg cgc acg aag ctt cgg gaa gcg cgc acc aat atg Gin Glu Leu Arg Thr Lys Leu Arg Glu Ala Arg Thr Asn Met gat gag Asp Giu gct tct cgc Ala Ser Arg tac atc cgc Tyr Ile Arg acg cga gaa aac gca gcc atc att gcc aac Thr Arg Glu Asn Ala Ala Ilie Ile Ala Asn 30 tca aag cag cca aaa agg att gcc gct tac Ser Lys Gin Pro Lys Arg Ilie Ala Ala Tyr gtt tct tat Val Ser Tyr gtg ccg gtg Val Pro Val cgt acc Arg Thr gaa cct ggt ggg Glu Pro Gly Gly ttg ctt ctt gac Leu Leu Leu Asp ctc cac gcc gaa Leu His Ala Glu 211 259 307 355 403 act Thr tcc gcg ctt att Ser Ala Leu Ile ttg Leu cca gtc tcc ctc Pro Val Ser Leu gat cga cgc ctc Asp Arg Arg Leu tgg gct ctt tat Trp Ala Leu Tyr ggc cca acc agc Gly Pro Thr Ser gtt cct ggc gca Val Pro Giy Ala ttt ggc Phe Gly 100 atc cag gaa Ile Gin Glu tgc gac ctt Cys Asp Leu 120 ccc ggt ggc act cgc ctt gga cct gaa gct ctc aac ttc Pro Gly Gly Thr Arg Leu Gly Pro Glu Ala Leu Asn Phe 105 110 115 gtc atc gcc ccc Val Ile Ala Pro gca Ala 125 ctg gca tgc acc Leu Ala Cys Thr ccc Pro 130 agt gga atc Ser Gly Ile agg cta ggt aaa ggt ggc ggt ttc tac gac cgc gct cta gcc acc gga Arg Leu Gly Lys Gly Gly Gly Phe Tyr ASP Arg Ala Leu Ala Thr Gly WO 01/00843 PCTIIBOO/00923 135 140 145 gtc aag gct gac gtg att act ttg ctc ttc aac gga gaa atc cgc gac 595 Val Lys Ala Asp Vai Ile Thr Leu Leu Phe Asn Gly Giu Ile Arg Asp 150 155 160 165 gat atc 601 ASP Ile <210> 676 <211> 167 <212> PRT <213> Corynebacterium giutamicum <400> 676 Met Ser Gin Thr Lys Gin Glu Leu Arg Asn Asn Tyr *Leu Arg Giy Ala Pro 130 Leu Giu Met Val Val1 His Arg Ala Leu 115 Ser Ala Ile Asp Ser Pro Ala Leu Phe 100 Asn Gly Thr Arg Thr Arg Lys Giy Ile Giu Giy Ile Gly Val Lys Giu Gin G ly Leu Gly Gly Ala Gly Ile 155 <210> 677 <211> 595 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101). .(595) <223> FRXA02027 WO 01/00843 WO 0100843PCTJBOOI00923 <400> 677 tcacgtgctc atcgataggc aaactcatgg aaccaagagt tcgtcgtacc cctccgaaaa taacggttat ccttagattt acctgccccc gcaaccccaa atg Met 1 agc caa act Ser Gin Thr cag gaa ctg cgc Gin Glu Leu Arg aag ctt cgg gaa Lys Leu Arg Glu cgc acc aat atg Arg Thr Asn Met gat gag Asp Glu gct tct cgc Ala Ser Arg tac atc cgc Tyr Ile Arg acg Thr cga gaa aac gca Arg Giu Asn Ala atc att gcc aac gtt tct tat Ile Ile Ala Asn Val Ser Tyr tca aag cag cca Ser Lys Gin Pro agg att gcc gct Arg Ile Ala Ala gtg ccg gtg Val Pro Val cgt acc Arg Thr gaa cct ggt ggg Glu Pro Gly Gly ttg ctt ctt gac Leu Leu Leu Asp gcc Ala ctc cac gcc gaa Leu His Ala Glu act Thr tcc gcg ctt att Ser Ala Leu Ile cca gtc tcc ctc Pro Val Ser Leu gat cga cgc ctc Asp Arg Arg Leu tgg gct ctt tat Trp Ala Leu Tyr gaa Glu ggc cca acc agc Gly Pro Thr Ser gtt cct ggc gca Val Pro Gly Ala ttt ggc.

Phe Gly 100 atc cag gaa Ile Gin Glu tgc gac ctt Cys Asp Leu 120 ggt ggc act cgc Gly Gly Thr Arg gga cct gaa gct Gly Pro Glu Ala ctc aac ttc Leu Asn Phe 115 agt gga atc Ser Gly Ile gtc atc gcc ccc Vai Ilie Ala Pro ctg gca tgc acc Leu Ala Cys Thr ccc Pro 130 agg cta Arg Leu 135 ggt aaa ggt ggc Gly Lys Gly Gly ttc tac gac cgc Phe Tyr Asp Arg cta gcc acc gga Leu Ala Thr Gly aag gct gac gtg Lys Ala Asp Val att Ile 155 act ttg ctc ttc Thr Leu Leu Phe gga gaa atc cgc Gly Giu Ile Arg gac Asp 165 <210> 678 <211> 165 <212> PRT <213> Corynebacterium glutainicum <400> 678 Met Ser Gin Thr Lys Gin Glu Leu Arg Thr 1 5 10 Lys Leu Arg Giu Ala Arg Thr Asn Met Asp Glu Ala Ser Arg Thr 25 Arg Glu Asn Ala Ala Ile Ile Ala Asn Val Ser Tyr Tyr Ile Arg Ser Lys Gin Pro Lys Arg Ile Ala WO 01/00843 Ala Tyr Val Ala Leu His Asp Arg Arg Pro Gly Ala Glu Ala Leu 115 Thr Pro Ser 130 Ala Leu Ala 145 Gly Glu Ile PCT/EBOO/00923- Gly Leu Gly Gly Ala Gly Ile 155 <210> 679 <211> 579 <212> DNA <213> Corynebacterium glutamicum <22 0> <221> CDS <222> (101)..(556) <223> RXA00106 <400> 679 tggagctcaa caaggcagcg tacatgtttg agtacagctt gctacgatcc acacccattg atccgcggca aggtcgccgt tgg Trp, tgg Trp, cag Gin ccg Pro gac ggc Gly ccg Pro atc Ile Gly gcc cgt Arg gaa Glu atg Met cgc Arg ggC a tc Ile cac His 30 acg Thr att Ile gtc 951 cgatgacatc accgtgtccg atg atc ggt gcg att Met Ile Gly Ala Ile 1 ggc acc gac atg ccc Gly Thr Asp Met Pro aaa acc acc atg ggc Lys Thr Thr Met Gly tct ttg ccg ttc aag Ser Leu Pro Phe Lys tca cgc gag ccc ggc Ser Arg Glu Pro Gly atc cct aaa agc ggc WO 01/00843 WO 0100843PCTAiBOO/00923 Asp Trp Ser Ala Gly Gly Thr Val 75 tgg atc atg ggc ggc ggc gag gtc Trp Ile Met Gly Gly Gly Giu Val gac gtt tta gaa ata acg ctt atc Asp Val Leu Giu Ile Thr Leu Ile 105 ccc gtc tac gca ccc gaa atc ccg Pro Val Tyr Ala Pro Giu Ilie Pro 120 125 tcc gag tgg ttt acc tca ggc gag Ser Giu Trp, Phe Thr Ser Gly Giu 135 140 atc aag gtt taaggagcaa acaacatga Ile Lys Val 150 <210> 680 <211> 152 <212> PRT <213> Corynebacteriun giutaniicum Val Thr Giu Ile tac aag gcc acc Tyr Lys Ala Thr 95 gac gcc acc ttc Asp Ala Thr Phe 110 gcg aac ttc aac Ala Asn Phe Asn tat cgt tac aag T'yr Arg T1yr Lys 145 g caa Pro Lys Ser agc Ser 100 tcc Ser gac Asp cgc Arg <400> 680 Met Ilie Gly Ala Ilie Trp Ala Gin Gly 1 Gly Lys Ser Ser Ilie Thr Phe Asn Lys 145 Asp Thr Pro Giu Lys Gly Val1 115 Asp Gin Trp Gin Pro Asp 70 Trp Asp Pro Ser Ile 150 Arg Giu Met Arg Giy Gly Ile Pro Thr Asp Gly Ilie Ile Gly Asp Asp Gly Giu Gly Gly Thr Giu Ser Leu Arg Asn Thr Giu Leu Ile Gly 140 Lys Arg Phe Val1 Val1 Ile Pro 125 Glu His Thr Ile Vai Tyr Asp 110 Ala Tyr WO 01/00843 WO 0100843PCTIBOO/00923 <210> 681 <211> 1044 <212> DNA <213> Corynebacterium glutamicum <220> <221> <222> <223>

ODS

(101)..(1021) RXN01321 <400> 681 ggagggtgta gtcaaggatt tgggccatgg tggagcggga aatcgtcata tccataccct acttagacct gacttagtgt gggaaaattt ccagggtaga atg caa cga atg acc Met Gin Arg Met Thr ccg agt tct cct Pro Ser Ser Pro gtt cgt aat cgt Val Arg Asn Arg agc gct gcg cct Ser Ala Ala Pro gaa gag Glu Glu cgt cag ttt Arg Gin Phe gcg aag ttg Ala Lys Leu gtg Val1 ctc act ttt ggc Leu Thr Phe Gly cct gac tcc act Pro Asp Ser Thr gga att gtg Gly Ile Val att act gag Ile Thr Glu tcg tcg ttc cta gct gag cgt ggg ggt Ser Ser Phe Leu Ala Glu Arg Gly Gly gct gga Ala Gly tat ttc acg gat Tyr Phe Thr Asp gat tcg aat tgg Asp Ser Asn Trp ttc Phe ttt ac-t cgt cag Phe Thr Arg Gin gcg Ala att cgc gct gag tcg att gat acc acg Ile Arg Ala Glu Ser Ilie Asp Thr Thr gag cag ttg cgg Giu Gin Leu Arg gag ttc gct ccg Glu Phe Ala Pro gcg gag gag ttc Ala Glu Giu Phe ccg agg gct aag Pro Arg Ala Lys tgg agt Trp, Ser 100 ttc act gac Phe Thr Asp gag ggc cac Glu Gly His 120 act Thr 105 gcg cag gtg aag Ala Gin Val Lys gct gtg ttg ttg Ala Val Leu Leu tgc ttg cac gat ttg tta ggt cgt gtg Cys Leu His Asp Leu Leu Gly Arg Val 125 gct Ala 130 gtg tct aag Val Ser Lys 115 gag aat gat Glu Asn Asp aac ttg cgt Asn Leu Arg tat ccg Tyr Pro 135 atg gaa gtt gtt gcg gtt gtg ggt aac Met Giu Val Val Ala Val Val Gly Asn 140 cat gag His Giu 145 tat Tyr 150 att gcg gag aac Ile Ala Glu Asn aat gtt ccg ttt Asn Val Pro Phe cat gtg ccg ttt His Val Pro Phe aag gat gcg gtt Lys Asp Ala Val ggt Gly 170 aag cgg aag gcg Lys Arg Lys Ala ttt Phe 175 gac cag gtc gct Asp Gin Val Ala gag att Glu Ile 180 WO 01/00843 gtg aat ggt Val Asn Gly PCTIBOO/00923 att ttg Ile Leu att cat Ile His 215 cag gcg Gin Ala 230 gcg act Ala Thr cgt gtg Arg Val gat gcg Asp Ala gac cgg 1021 Asp Arg 295 tat Tyr 185 ccg Pro agt Ser agc Ser ga t Asp cat His 265 aag Lys c tg ccg Pro t tg Leu ttg Leu gg t Gly 235 gat Asp gat Asp gtg Val tac gat Asp tgt Cys ccg Pro 220 gtg Val ga t Asp acg Thr ctg Leu ggt Gly 300 gcg Al a gag Giu 205 tcg Ser aag Lys ggt Gly ccg Pro gc t Ala 285 aa c Asn a tg Met ttg Leu tat Tyr cat His gtt Val 260 ggc Gly t tg Leu Pro Pro Arg Asn Thr Gly Val Leu Val Tyr taaggctttt tgcttttcga cgc 1044 <210> 682 <211> 307 <212> PRT <213> Corynebacterium glutanicum <400> 682 Met Gin Arg Met Thr Pro Ser Ser Pro 1 5 Ala Ala Pro Glu Glu Arg Gin Phe Val Ser Thr Gly Ilie Val Ala Lys Leu Ser Gly Trp Ilie Thr Glu Ala Gly Tyr Phe Phe Phe Thr Arg Gin Ala Ile Arg Ala Giu Gin Leu Arg Glu Glu Phe Ala Pro WO 01/00843 WO 0100843PCT/EBOO/00923 Arg Leu Val1 His 145 His Gin Ala Gly Ala 225 Ala Giu Gin Arg Val1 305 Ala Leu Ala 130 Glu Val1 Vai Arg Arg 210 Arg Thr Gin Arg Phe 290 Phe Lys Val1 115 Giu Asn Pro Ala Phe 195 Val1 Pro Cys Asp Leu 275 His Asp Trp Ser Phe Thr Asp Thr Ala Gin Val Lys Lys Ala Val 100 105 110 Ser Asn Leu Phe Giu 180 Met Leu Tyr His Val 260 G ly Leu Lys Asp Arg Pro 165 Ile Gin Asn His Tyr 245 Ile Arg Glu His Val1 His 155 Lys Pro Leu Leu Gly 235 Asp Asp Val1 Tyr Asp Ala 140 Asn Arg Asp Cys Pro 220 Val Asp Thr Leu Gly 300 Leu 125 Val Val1 Lys Ala Giu 205 Ser Lys G ly Pro Ala 285 Asn Leu Val1 Pro Ala Ile 190 Met Phe Leu Pro Thr 270 Arg Arg Gly Arg Gly Asn Phe Phe 160 Phe Asp 175 Val Leu Trp, Ala Met Gly Ile Giy 240 Ile Ile 255 Giu Met Gly Leu Thr Val <210> 683 <211> 582 <212> DNA <213> Corynebacterium giutainicum <22 0> <221> CDS <222> (23)..(559) <223> FRXA01321 <400> 683 cttgcacgat ttgttaggtc gtgtg gct gag aat gat tat ccg atg gaa gtt 52 Val Ala Giu Asn Asp Tyr Pro Met Giu Val 1 5 gtt gcg gtt gtg ggt aac cat gag aac ttg cgt tat att gcg gag aac 100 Val Ala Val Val Gly Asn His Giu Asn Leu Arg Tyr Ile Ala Giu Asn 20 WO 01/00843 WO 0100843PCTIBiOO/00923 cat aat gtt His Asn Val aag Lys ccg Pro t tg Leu ttg Leu gg t Giy gat Asp ga t Asp gtg Val1 155 aag Lys gcg Ala gag Giu tcg Ser aag Lys ggt Gly 125 ccg Pro gct Ala ccg Pro gcg Ala att Ile atg Met ttt Phe t tg Leu 110 ccg Pro act Thr cgc Arg ttt ttc cat gtg Phe Phe His Val ccg ttt cct Pro Phe Pro 35 gct gag att Ala Giu Ile ttt atg cag Phe Met Gin gtg ttg aat Val Leu Asn ccg tat cat Pro T[yr His 100 tgc cat tat Cys His Tyr 115 gat gtt att Asp Vai Ile ttg ggc cgc Leu Gly Arg cac ttg gag His Leu Giu 165 gtg Val att Ile att Ile cag Gin gcg Ala cgt Arg gat Asp 150 gac aag gat Lys Asp aat Asn t tg Leu cat His gcg Ala act Thr gtg Val 135 gcg Ala C99 gcg Aia gg t Gly ccg Pro cac His tat Tyr ggg Gly 120 acg Thr gag Glu gtg gtt Val1 tat Tyr ccg Pro agt Ser agc Ser 105 ga t Asp cat His aag Lys ctg Asp Arg Vai Leu Vai tac ggt aac cgc acg gtt gtc ttt gat taaggctttt tgcttttcga Tyr Gly Asn Arg Thr Vai Val Phe Asp 175 cgc <210> 684 <211> 179 <212> PRT <213> Corynebacterium giutanicun <400> 684 Vai Ala Giu Asn Asp Tyr Pro Met Giu Val Val Ala Vai Val Giy Asn 1 5 10 His Giu Asn Leu Arg Tyr Ile Ala Giu Asn His Asn Val Pro Phe Phe 25 His Val Pro Phe Pro Lys Asp Ala Val Gly Lys Arg Lys Ala Phe Asp 40 Gin Val Ala Glu Ile Val Asn Giy Tyr Asp Pro Asp Aia Ile Vai Leu 55 WO 01/00843 WO 0100843PCT/IBOO/00923 Ala Gly Ala Ala Giu Gin 145 Arg Val1 Arg Arg Arg Thr Gin 130 Arg Phe Phe Phe Val1 Pro Cys 115 Asp Leu His Asp Met Leu Tyr 100 His Val Gly Leu Gin Asn His Tyr Ile Arg Giu 165 Pro His Tyr Gly 120 Thr Giu Val1 Pro Ser Ser 105 Asp His Lys Leu Asp Phe Arg Leu Lys Gin Val 170 Cys Pro Val1 Asp Thr 140 Leu Gly Ala Gly Gly Ile Met Leu 160 Val1 <210> 685 <211> 975 <212> DNA <213> Corynebacteriun giutamicun <220> <221> CDS <222> (952) <223> RXA00461 <400> 685 tgttgggagg gatgacagga ttgtcgaaga taacgtgaag tgattgtaag gccttggaaa agggtggaat aatagcgggc ctt gat Leu Asp cgc gtt Arg Val gtg Val cat His cct Pro tac Tyr aag Lys gac Asp cag Gin cag Gin cgc Arg gag Giu cca Pro att Ile gaa Glu gaa Giu ggg Gly agc Ser gtg Val ctt Leu 957 tgggtgttcc ggcatgtgtt gtg act gca atc aaa Val Thr Ala Ile Lys 1 gcc gac ttg gaa cag Ala Asp Leu Giu Gin ccg ggg ctt gcc acc Pro Gly Leu Ala Thr tac gtg aag atg aag Tyr Val Lys Met Lys atc cgt aag gat ctg Ile Arg Lys Asp Leu gtc atc gat gaa ctg Val Ile Asp Giu Leu WO 01/00843 WO 0100843PCTAIB0O/00923 aac aac gat gat Asn Asn Asp Asp tgc act ggt tac Cys Thr Gly Tyr gtg cag ctt cct Val Gin Leu Pro ttg cct Leu Pro 100 aag cac ttg Lys His Leu gaa aac gct gtg Giu Asn Ala Val gag cgc att gat Glu Arg Ile Asp cca gct aag Pro Ala Lys 115 gtg ctc aac Val Leu Asn gat gct gat ggc ctg cac cct gta aac ctg ggc aag Asp Aia Asp Gly Leu His Pro Val Asn Leu Gly Lys gag cca Giu Pro 135 gct cca ctg cca Ala Pro Leu Pro tgc Cys 140 acc ccg aat ggt Thr Pro Asn Giy atc agc ttg ttg Ile Ser Leu Leu cgt cgt ttc ggc gtt gag ctt gat ggc gcg aag gtt gtt gtc att ggc Arg Arg Phe Gly Val Glu Leu Asp Gly Ala Lys Val Val Val Ile Gly 150 155 160 165 cgt ggc gtc acc Arg Gly Val Thr gtt Val 170 ggt cgc cca att Gly Arg Pro Ilie ctg atg ctg acc Leu Met Leu Thr cgc cgt Arg Arg 180 tcc gag aac Ser Glu Asn gct gcg gag Ala Ala Glu 200 acg gtt act ttg Thr Val Thr Leu cac act ggc acg His Thr Gly Thr aag gat ctg Lys Asp Leu 195 gct ggt cag Ala Gly Gin acc cgt gcg gct Thr Arg Ala Ala gac Asp 205 gtc atc att gct Val Ile Ilie Ala ccg cac Pro His 215 atg ctg acc gca Met Leu Thr Ala gac Asp 220 atg gtc aag cca Met Val Lys Pro ggC Gly 225 gca gcg gtg ctc Ala Ala Val Leu gat Asp 230 gtc ggc gtc tcc Val Gly Val Ser cgc Arg 235 aag gac ggc aag Lys Asp Gly Lys ctt ggc gac gtc Leu Gly Asp Val cac His 245 ccc gac gtg tgg Pro Asp Val Trp gaa Giu 250 gtc gcc ggc gcg Val Ala Gly Ala gtc Val1 255 tca cca aac cca Ser Pro Asn Pro ggc ggc Gly Gly 260 gtt ggc cct Val Gly Pro gct gaa aag Ala Giu Lys 280 acc cgt gca ttc Thr Arg Ala Phe t tg Leu 270 gtg cac aat gtt Val His Asn Val gtc gag cgc Val Glu Arg 275 ctg gct gga ctc Leu Ala Gly Leu taaaaacaca tgactaatcc cgg <210> 686 <211> 284 <212> PRT <213> Corynebacterium giutamicum <400> 686 WO 01/00843 PCT/1BOO/00923 Ile Phe Val1 Ala Pro Tyr Ile Val Gin Ile Lys Ser 145 Val1 Met Gly Ala Gly 225 Leu Pro Asn Thr

ASP

G ly Val1 Arg Ile Leu Asp Leu 130 Ile Val1 Leu Thr Ala 210 Ala Gly Asn Val1 Ala Leu Leu Lys Lys Asp Pro Pro 115 Val Ser Val Thr Lys 195 Ala Ala Asp Pro Val1 275 Ile Lys Leu Asp Giy Asn Leu Tyr Arg Gly Glu 10 Glu Ala Met Asp Glu Leu 100 Ala Leu Leu Ile Arg 180

ASP

Gly Val Val Gly 260 Glu Gin Thr Lys Leu Leu Pro Lys Asn Leu Gly 165 Arg Leu Gin Leu His 245 Gly Arg Arg Val His Pro 70 Asn Lys Asp Giu Arg 150 Arg Ser Ala Pro Asp 230 Pro Val Ala Val Leu Arg 55 Ala Asn His Ala Pro 135 Arg Gly Glu Ala His 215 Val Asp Giy Glu Al a Val1 40 Asp Asp Asp Leu Asp 120 Ala Phe Val1 Asn Giu 200 Met Gly Val Pro Lys 280 Ala 25 G ly Cys Val1 Asp Asp 105 Gly Pro Gly Thr Ser 185 Thr Leu Val1 Trp Leu 265 Leu Leu Asp Giu Thr Ser 90 Glu Leu Leu Val1 Val1 170 Thr Arg Thr Ser Giu 250 Thr Ala Giu Pro Ile Giu Thr Ala Pro Cys 140 Leu Arg Thr Ala Asp 220 Lys Ala Ala Leu Lys Ala Gly Glu Gly Val Val 125 Thr Asp Pro Leu Asp 205 Met Asp Gly Phe Gly Ser Val1 Leu Tyr Leu 110 Asn Pro Gly Ile Cys 190 Val1 Val Gly Ala Leu 270 Ile His Asn Phe Ile Glu Leu Asn Ala Gly 175 His Ile Lys Lys Val1 255 Val1 Val1 Ser Ser Ala Val Arg Gly Gly Lys 160 Leu Thr Ile Pro Leu 240 Ser His <210> 687 <211> 711 <212> DNA <213> Corynebacterium giutamicum <220> WO 01/00843 WO 0100843PCTA~BOO/00923 <221> CDS <222> (101)..(688) <223> RXA01514 <400> 687 accacagaaa tgcctgtcgt tccagatcag cccatcgatg gtgattccgg gaagtccgct gagggcacac aggagaatcc ggaaaatgaa ggagacaacc gtg gat aac cac Val Asp Asn His gca gtt cgc gag Ala Val Arg Giu t tc Phe gat gag gag cgc gca aca gct gcg att Asp Giu Giu Arg Ala Thr Ala Ala Ile cgt gag Arg Giu ttg ctc atc gct gtg ggt gag gat Leu Leu Ile Ala Val Gly Glu Asp cca gat cgc gaa ggc ctg ttg gaa Pro Asp Arg Glu Gly Leu Leu Glu 30 115 163 211 259 307 355 acc cca gct cga gtg gct agg Thr Pro Ala Arg Val Ala Arg cat gag gat ccc acc act gtg His Glu Asp Pro Thr Thr Val gcg Ala tac aag gaa act Tyr Lys Glu Thr gcg ggt ctg Ala Gly Leu ctg gag aag acg Leu Giu Lys Thr tct gag ggC cat Ser Giu Gly His gaa Giu gag ttg gtt ctg Glu Leu Val Leu gtt cgt gag atc ccg att tac tcc atg tgt gag Val Arg Glu Ile Pro Ile Tyr Ser Met Cys Giu 75 80 cac cac ttg gtg His His Leu Val ttc ttt ggc gtg Phe Phe Gly Val cac att ggt tac His Ile Gly Tyr att. ccg Ile Pro 100 ggt aag tcc Gly Lys Ser gat atg ttt Asp Met Phe 120 aag gtg act ggc Lys Val Thr Gly ctg Leu 110 tcc aag ctg gcg Ser Lys Leu Ala cgt tta gcg Arg Leu Ala 115 acc tcc caa Thr Ser Gin gct aag cga cct Ala Lys Arg Pro cag Gin 125 gtt cag gag cgc Val Gin Giu Arg att gcg Ile Ala 135 gat gct ctc gtc Asp Ala Leu Val aag ctt gat gcc cag gcc gtg gcc gtg Lys Leu Asp Ala Gin Ala Val Ala Val 145 gtg att gaa gct gag Val Ilie Giu Ala Giu 150 ctg tgc atg gcc Leu Cys Met Ala cgc gga atc cgt Arg Gly Ile Arg cct ggt gct gtg Pro Gly Ala Val acg acg tct gcg Thr Thr Ser Ala cgc ggC ggt ttt Arg Gly Gly Phe aag aac Lys Asn 180 aac gct gcc Asn Ala Ala cgc gct gag gtg Arg Ala Giu Val tcc ctg att cgg Ser Leu Ile Arg ggg cac Gly His 195 taaatgaacg tatcctcttt gac WO 01/00843 WO 0100843PCTIBOOIOO923 <210> 688 <211> 196 <212> PRT <213> Corynebacterium giutaniicum <400> 688 Vai Asp Asn His Ala Ala Vai Arg Glu 1 Ala Glu Thr Phe Tyr Ilie Leu Arg Gin 145 Arg Gly Ile Arg Leu Giy Giy Cys Ile 100 Leu Ser Ala Arg Lys 180 His 5 Glu Glu Leu His Glu Pro Ala Gin Val1 Lys 165 Asn Leu Thr His Glu 70 His Giy Asp Ile Vai 150 Pro Asn Leu Pro Glu Giu His Lys Met Ala 135 Ile Giy Ala Ile Ala Asp Leu Leu Ser Phe 120 Asp Giu Ala Ala Aia 25 Arg Pro Val Vai Giy 105 Ala Ala Ala Val Ser 185 Phe 10 Val1 Val1 Thr Leu Pro 90 Lys Lys Leu Giu Thr 170 Arg Asp Gly Ala Thr Val1 75 Phe Val1 Arg Val1 His 155 Thr Ala Giu Giu Arg Val1 Arg Phe Thr Pro Giu 140 Leu Thr Glu Arg Pro Tyr Glu Ile Val1 Leu 110 Val1 Leu Met Aia Phe 190 Ala Asp Lys Lys Pro Ala Ser Gin Asp Ala Val1 175 Ser Thr Arg Giu Thr Ile His Lys Giu Ala Met 160 Arg Leu <210> 689 <211> 513 <212> DNA <213> Corynebacterium giutaiicun <220> <221> CDS <222> (490) <223> RXA01516 <400> 689 tctgcacata tgggagcatg gggtgtgcgc gtgcacgatg tcccagtatc aagggacgct gttgatgttg ccgcattgtg gcgaagtgga ggaactcacc atg gct gat cgt att 115 961 WO 01/00843 gaa Glu gaa Glu gat Asp tac Tyr agg Arg gc t Ala aaa Lys cg t Arg ctt Leu aaa Lys t tc Phe ggc Gly gat Asp aaa Lys gca Ala tcc Ser aaa Lys gag Glu ga t Asp gcg Ala ttg Leu ttt Phe ccg Pro agg Arg ggc Gly c aa Gin gcc Ala t tg Leu atc Ile gat Asp atc Ile 105 aaa Lys ttc Phe ttc Phe agc Ser gtt Val1 gcc Ala gcg Ala ttt Phe gga Gly att Ile gat Asp gc t Ala acg Thr g tg Val1 gc t Ala 110 gga Gly cac cac His His gtg gat Val Asp gac ctt Asp Leu gaa atc Glu Ile gaa tct Glu Ser 80 gaa gta Glu Val gac gtc Asp Val Met 1 ggt Gly gtc Val tcc Ser gtg Val gcg Ala acc Thr gcg Ala PCTIIBOO/00923 Arg le gac ttt 163 Asp Phe tgg atg 211 Trp Met gta gat 259 Val Asp cca tcc 307 Pro Ser gtg atg 355 Val Met aag ccc 403 Lys Pro 100 gcc cga 451 Ala Arg tcc atg gct gct Ser Met Ala Ala agg agc aac gcc taatgcatgc Arg Ser Asn Ala 130 120 125 agftttgtcc atc <210> 690 <211> 130 <212> PRT <213> Corynebacterium glutamicum <400> 690 Met Ala Asp Arg Ile Glu Leu Lys Gly 1 5 Gly Val Phe Asp Phe Glu Lys Glu Gin Val Thr Cys Trp Met Asp Phe Asp Ala 40 Ser Asp Thr Val Asp Tyr Gly Ala Leu Val Glu Gly Pro Ser Arg Asp Leu Ile 70 Ala Asp Ala Val Met Ala Lys Phe Asp Leu 10 Gly Al a Ala Glu Ala 90 Glu Gin Gly Leu Thr Leu Phe Phe Ser Val1 Ala Ala His Val Asp Glu Glu Glu WO 01/00843 WO 0100843PCT/IBOO/00923 Thr Ile His Lys Pro Lys Ala Pro Ile Pro Arg Thr Phe Ala Asp Val 100 105 110 Ala Val Val Ala Arg Arg Ser Arg Lys Ser Met Ala Ala Gly Arg Ser 115 120 125 Asn Ala 130 <210> 691 <211> 975 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (101)..(952) <223> R.XA01515 <400> 691 taagcctggt gctgtgacca cgacgtctgc ggtgcgcggc ggttttaaga acaacgctgc ctcccgcgct gaggtgttct ccctgattcg ggggcactaa atg aac gta tcc tct 11 Met Asn Val Ser Ser t tg Leu gag Glu atc Ile gtc Val gtg Val1 ggc G ly gcc Ala gct Ala acc Thr gat Asp gcg Ala ggc Gly gaa Glu a tc Ile gcg Ala gat Asp ttg Leu ggC Gly gtg Val1 cc t Pro ccg Pro acc Thr atg Met gtc Val 125 gtc Val aag Lys gc t Ala ggg G ly gtc Val a tg Met atc Ile 110 atg Met 1 att Ile gac Asp gcc Ala cgc Arg 65 gcg Ala tcc Ser gtc Val1 gcg Ala act Thr gcg Ala gat Asp t cc Ser gcc Ala gct Ala ttg Leu gtg Val1 tgt ttg atg cac tgg cgc acc ctc caa ttc ggt gat gcc gca ggt cag WO 01/00843 Cys Leu Met 135 gca gat cac Ala Asp His 150 gat ctt gtc Asp Leu Val gtg ctt gat Val Leu Asp cgt ttg ctg Arg Leu Leu 200 ctg gtg gga Leu Val Giy 215 cgt ggc cta Arg Giy Leu 230 gtg acc gca Val Thr Ala gat gtc cca Asp Val Pro Trp gga Gly cgc Arg 170 ggt Giy gca Ala tcc Ser gtc Val1 tct Ser 250 tca Ser Arg gac Asp 155 gcc Ala t tg Leu c tg Leu cgg Arg acc Thr 235 gca Ala agg Arg Thr Leu Gin 140 gtt gta gcc Val Val Ala acc gct gct Thr Ala Ala ggt ttt gcc Gly Phe Ala 190 ccc gag ttt Pro Glu Phe 205 aag cga ttc Lys Arg Phe 220 ccc att gat Pro Ilie Asp cat atg gga His Met Gly gac gct gtt Asp Ala Val 270 Phe gat Asp ggt Gly 175 aaa Lys att Ile c tg Leu gcc Ala gca Ala 255 gat Asp 145 cac His gcc Ala cgt Arg gga Gly ggc G ly 225 c ca Pro ggt Gly gcc PCTLBOOIOO923 Gly Gin ctt gat 595 Leu Asp 165 cag atc 643 Gin Ile 180 aac tgg 691 Asn Trp ccc atc 739 Pro Ile aaa gac 787 Lys Asp gca gcg 835 Ala Ala 245 gtg cac 883 Val His 260 tgg cga 931 Trp Arg 975 Asp Val Ala Ala Leu agt gga gga act cac cat ggc tgatcgt Ser Gly Gly Thr His His Gly 280 <210> 692 <211> 284 <212> PRT <213> Corynebacterium giutamicum <400> 692 Met Asn Val Ser Ser Leu Thr Ile Pro 1 5 Ile Val Asn Val Thr Glu Asp Ser Phe 25 Asp Val Asp Gin Ala Ilie Ala His Ala 40 Ala Asp Met Ilie Asp Val Gly Gly Giu 55 Arg Val Asp Ala Ser Val Giu Arg Asp 70 att gaacttaaag gcc Gly Arg Cys Leu Val met Gly 10 Ser Asp Gly Gly Lys Tyr Ile Lys Glu Leu Val Ala Aia Gly Ser Thr Arg Pro Gly Ala Val Arg Val Val Pro Val Ile Lys 75 WO 01/00843 Ala Leu His Ser Val Ala Val Ser Gly 115 Ala Gin Ile 130 Asp Ala Ala 145 His Ala Vai Ala Glu Asn Arg Glu Asp 195 Gly Pro Phe 210 Gly Val Arg 225 Pro'Ala Thr Gly Val Arg Ala Ala Leu 275 PCTIIBOO/00923 Ala Ala Leu Val1 Gln Asp 165 Ile Trp Ile Asp Ala 245 His Arg Gly Ala Ala Cys Al a 150 Asp Val Arg Leu Arg 230 Val1 Asp Ser His Gly Pro 120 Met His Val1 Asp Leu 200 Gly Leu Ala Pro Gly 280 Ser Gly Met Trp Gly Arg 170 Gly Ala Ser Val Ser 250 Ser His Met Ile 110 Met Gin Ala Ala Ala 190 Phe Phe Asp Gly Val1 270 <210> 693 <211> 859 <212> DNA <213> Corynebacterium glutainicum <22 0> <221> CDS <222> (859) <223> RXA02024 <400> 693 cactgatgac ctggatcagg ccgtcaaatt catcgtcgat gcacacgctg gattggacgt agcgcgtctc cacaattaag cagtggctac attaggtgtt atg agt tct ttg ccg 115 Met Ser Ser Leu Pro 1 gtc atc atg gcc atc gtc aat cgc acc ccg gat tct ttc tat gac aag 163 Val Ile Met Ala Ile Val Asn Arg Thr Pro Asp Ser Phe Tyr Asp Lys 15 WO 01/00843 ggt gcg aca Gly Ala Thr att gaa caa Ile Glu Gin PCTIIBOO/00923 gag gac acc gct Glu Asp Thr Ala cta aac agg gca Leu Asn Arg Ala gcg gag gtc Ala Glu Val ggc gcc ggc att Gly Ala Gly Ile gtc gat atc ggt ggg gtg aaa gcc ggc Val Asp Ile Gly Gly Val Lys Ala Gly 45 ccg ggg Pro Gly gat ttc gtg tcg gcg gag gaa gag atc Asp Phe Val Ser Ala Glu Glu Glu Ile cgc gtg gtg cca Arg Val Val Pro atc gct gcg gtg Ile Ala Ala Val cga Arg gaa cgt ttt cct Giu Arg Phe Pro att gat att tct Ile ASP Ile Ser gat acc tgg cgg Asp Thr Trp Arg tcg gtg gct gat Ser Val Ala Asp gca gtg gcg cat Ala Val Ala His gga gca Gly Ala 100 acg ctg atc Thr Leu Ilie gta gca ggg Val Ala Gly 120 gac act tgg gcc Asp Thr Trp Ala cat gat cat gag His Asp His Glu ttg gtg cag Leu Val Gin 115 acc ggc ggg Thr Gly Gly cag cac aag gtg Gin His Lys Val tat gtc tgc tcg Tyr Val Cys Ser cac His 130 gtg att cca aga acg cga cca tat cgg gtg cat ttc Val Ile Pro Arg Thr Arg Pro Tyr Arg Val His Phe 135 140 145 gat gac atc gtg Asp Asp Ile Val gat gta att acg Asp Val Ile Thr gag Giu 155 acc acc aaa ttg Thr Thr Lys Leu gag caa gct gtt Glu Gin Ala Val gcc ggg gtg cca Ala Gly Val Pro gag Glu 170 gaa cgg gtg ttt Glu Arg Val Phe gat ccc acc cat Asp Pro Thr His gat ttc Asp Phe 180 ggg aaa aac Gly Lys Asn gtg gtt gcc Val Val Ala 200 ttc cac gga ctg Phe His Gly Leu ctt tta cga cgg Leu Leu Arg Arg atc gat gag Ile Asp Glu 195 aat aag gat Asn Lys Asp acg ggc tgg ccg Thr Gly Trp, Pro gtg Val1 205 ctg atg gcc ttg Leu Met Ala Leu ag t Ser 210 ttc att Phe Ile 215 ggg gaa act ttg Gly Glu Thr Leu gaa Glu 220 agg ggc gtc gat Arg Gly Val ASP cgt gtt gct ggc Arg Val Ala Gly ctt gct gcc act Leu Ala Ala Thr gcc Ala 235 tgg gcg gcg gcg Trp Ala Ala Ala ggc gtt gcg gct Gly Val Ala Ala cgc gtg cat gaa Axg Val His Giu gtt Val1 250 gcg gaa acc 859 Ala Glu Thr WO 01/00843 PCTIiBOO/00923 <210> 694 <211> 253 <212> PRT <213> Corynebacterium glutamicui <400> 694 Met Ser Ser Leu Pro Val Ile Met Ala Ile Val Asn Arg Thr Pro Asp 1 5 10 Ser Phe Tyr Asp Lys Gly Ala Thr Phe Giu Asp Thr Ala Ala Leu Asn 25 Arg Ala Ala Glu Val Ile Giu Gin Gly Ala Gly Ile Val Asp Ile Gly 40 Gly Val Lys Ala Gly Pro Gly Asp Phe Val Ser Ala Giu Glu Glu Ile 55 Asp Arg Val Val Pro Ile Ile Ala Ala Val Arg Giu Arg Phe Pro Asp 70 75 Ile Asp Ile Ser Val Asp Thr Trp Arg Ala Ser Val Ala Asp Val Ala 90 Val Ala His Gly Ala Thr Leu Ile Asn Asp Thr Trp Ala Gly His Asp 100 105 110 His Glu Leu Val Gin Val Ala Gly Gin His Lys Val Gly Tyr Val Cys 115 120 125 Ser His Thr Gly Giy Val Ile Pro Arg Thr Arg Pro Tyr Arg Val His 130 135 140 Phe Asp Asp Ile Val Ala Asp Val Ile Thr Giu Thr Thr Lys Leu Ala 145 150 155 160 Glu Gin Ala Val Arg Ala Gly Val Pro Giu Glu Arg Val Phe Ile Asp 165 170 175 Pro Thr His Asp Phe Gly Lys Asn Thr Phe His Gly Leu Giu Leu Leu 180 185 190 Arg Arg Ile Asp Glu Val Val Ala Thr Gly Trp Pro Val Leu Met Ala 195 200 205 Leu Ser Asn Lys Asp Phe Ile Gly Glu Thr Leu Glu Arg Gly Val Asp 210 215 220 Lys Arg Val Ala Gly Thr Leu Ala Ala Thr Ala Trp Ala Ala Ala Arg 225 230 235 240 Gly Val Ala Ala Phe Arg Val His Glu Val Ala Glu Thr 245 250 <210> 695 <211> 579 <212> DNA <213> Corynebacteriun glutaricum <22 0> WO 01/00843 WO 0100843PCT/IBOO/00923 <221> CDS <222> (101). .(556) <223> RXA00106 <400> 695 tggagctcaa caaggcagcg tacatgtttg agtacagctt gctacgatcc acacccattg atccgcggca aggtcgccgt tgg gca caa ggc cgt gac ggc atc atc ggc gac Trp Ala Gin Gly Arg Asp Gly Ilie Ile Gly Asp 15 cgatgacatc accgtgtccg atg atc ggt gcg Met Ile Gly Ala 1 ggc acc gac atg Gly Thi- Asp Met ccc Pro tgg cac atc Ti-p His Ile cag ccg gtc Gin Pro Val ccg Pro gaa gac ctc aaa Giu Asp Leu Lys ttc aag aaa acc Phe Lys Lys Thr acc atg ggc Thr Met Giy atc atg ggt cgt Ile Met Gly Arg acg tgg gag tct ttg ccg ttc aag Thr Ti-p Giu Ser Leu Pro Phe Lys ccg ctt Pro Leu ccc ggc cgc gag Pro Gly Arg Giu ttc att ctc tcc Phe Ile Leu Ser tca Ser cgc gag ccc ggc Arg Giu Pro Gly gac Asp tgg tcc gcc ggc Ti-p Ser Ala Gly aca gtg gtc acc Thi- Val Val Thr atc cct aaa agc Ile Pro Lys Ser tgg atc atg ggc Ti-p Ile Met Gly ggc gag gtc tac Giy Giu Val Tyr gcc acc gtc ggc Ala Thi- Val Gly agc gcc Ser Ala 1 00 gac gtt tta Asp Val Leu ccc gtc tac Pro Vai Tyr 120 gaa Giu 105 ata acg ctt atc Ile Thr Leu Ile gcc acc ttc gat Ala Thi- Phe Asp gtt tcc act Val Ser Thi- 115 gat gac gaa Asp Asp Giu gca ccc gaa atc Ala Pro Giu Ile gcg aac ttc aac Ala Asn Phe Asn ctc Leu 130 tcc gag Ser Giu 135 tgg ttt acc tca Ti-p Phe Thr Ser gag tat cgt tac Giu Tyr Arg Tyr ttc cag cgc tac Phe Gin Ai-g Tyr atc aag gtt Ilie Lys Val 150 taaggagcaa acaacatgag caa <210> 696 <211> 152 <212> PRT <213> Corynebacterium giutamicum <400> 696 Met Ilie Gly Ala Ile Ti-p Aia Gin Giy Arg Asp Gly Ilie Ile Giy Asp 1 5 10 WO 01/00843 Gly Thr Asp Met Pro Trp His Ile Lys Thr Thr Met Gly Gin Pro Vai Ser Leu Pro Phe Lys Pro Leu Pro Ser Arg Giu Pro Gly Asp Trp Ser 70 Ile Pro Lys Ser Giy Trp Ile Met Thr Val Giy Ser Ala Asp Val LeuC 100 Phe Asp Val Ser Thr Pro Val Tyr 115 120 Asn Leu Asp Asp Glu Ser Giu Trp 130 135 Lys Phe Gin Arg Tyr Ile Lys Val 145 150 <210> 697 <211> 1556 <212> DNA <213> Corynebacterium giutanicuni <22 0> <221> CDS <222> (1)..(1533) <223> RXA00989 <400> 697 gga att ggc cta gtg gct aac aac Gly Ile Gly Leu Val Ala Asn Asn 3 1 5 gat gac atc acc gta ggc gat gtc z Asp Asp Ile Thr Vai Gly Asp Val ttg ccc atc gat ctc gcc ggt gag q Leu Pro Ile Asp Leu Ala Giy Giu 40 atc acc caa gaa gat ttg ctg cgc c Ile Thr Gin Giu Asp Leu Leu Arg I 55 gat caa cgt tgg ttg gaa acc aaa z Asp Gin Arg Trp Leu Giu Thr Lys I agc tac atg atg gat ctc atg ggc c Ser Tyr Met Met Asp Leu Met Gly C ?ro Ile ;1y kla ;iy ?he ;ac ~sp icg 'hr ~ta Ta1 :tt jeu itt le :aa ;in 969 Giu Met Arg G ly Gly 90 Ile Pro Thr gga Gly t tg Leu gaa Giu gcc Ala ga t Asp cca Pro PCTIBOO/00923 ae Lys rp Giu au Ser ,,ir Glu Ala La Thr 3n Phe r~g Tyr ttt gat Phe Asp gag act Giu Thr cct gcc Pro Ala gtg gaa Val Giu act ttc Thr Phe aat tcc Asn Ser WO 01/00843 WO 0100843PCT/IBOO/00923 att cac gtg Ile His Val ggc acc aac ggt Gly Thr Asn Gly acc tcc acc acc Thr Ser Thr Thr cgc atg atc Arg Met Ile 110 acc acc agc Thr Thr Ser gag tcg ttg ctg cgc gca ttc Glu Ser Leu Leu Arg Ala Phe cac His 120 cgc cgc acc ggc Arg Arg Thr Giy ccg cac Pro His 130 ctg cag ctg gta Leu Gin Leu Val acc Thr 135 gaa cgc atc gcg Giu Arg Ile Ala gat ggc aag ccc Asp Giy Lys Pro atc Ile 145 cac ccg cgt gat His Pro Arg Asp gtg cgg atc tac Vai Arg Ile Tyr gag att aag ccc Giu Ile Lys Pro tac Tyr 160 atg gag atg acc Met Giu Met Thr gcc tgg tca gag Ala Trp Ser Giu gcc Ala 170 gag ggc gga ccg Giu Gly Gly Pro aag atg LYS Met 175 agc aag ttt Ser Lys Phe gct cct gtt Ala Pro Val 195 gca ctc gtg gcc Ala Leu Val Ala ctc Leu 185 gct tac gca ggt Ala Tyr Ala Gly ttt gcc gac Phe Aia Asp 190 gga cgc tgg Gly Arg Trp gac gtc gcc gtc Asp Val Ala Val gt t Val1 200 gag gtt ggt ctt Giu Val Gly Leu ggc Gly 205 gat gcc Asp Ala 210 act aac gtg atc Thr Asn Val Ile aac Asn 215 gca gct gtt tcc Ala Ala Val Ser atc acc ccg gtg Ile Thr Pro Val ggc Gly 225 atg gac cac gtg Met Asp His Val gat Asp 230 cgc ctg ggc aac Arg Leu Gly Asn att ggt gaa atc Ile Gly Giu Ile gc t Ala 240 ggt gaa aag gcc ggc atc atc aag gct cgt Gly Giu Lys Ala Gly Ile Ile Lys Ala Arg 245 250 cct gca tct gag Pro Ala Ser Glu gat ggc Asp Gly 255 acc gag cct Thr Giu Pro gca atg aac Ala Met Asn 275 gag Glu 260 ggc aac gtt gtc Gly Asn Val Val a tc Ile 265 gtg ggc aag cag Val Gly Lys Gin gag cca gaa Glu Pro Giu 270 gca gct gtt Ala Ala Val gtg att ctg cag Val Ile Leu Gin caa Gin 280 gcc gtg gac gtg Ala Val Asp Val gac Asp 285 gct cgt Ala Arg 290 ttg aac atg gaa Leu Asn Met Giu t tc Phe 295 ggc gtg gtg gaa Gly Val Val Giu gcc att gcc gtt Ala Ile Ala Val gg t Gly 305 gga cag cag ctc Gly Gin Gin Leu acc Thr 310 ctg aag ggt ttg Leu Lys Gly Leu ggc gaa tac acc Gly Glu Tyr Thr gac Asp 320 atc ttc ctc cca ctg tct ggc gcg cac caa gca gat aat gcc gcg gtt 1008 Ilie Phe Leu Pro Leu Ser Gly Ala His Gin Ala Asp Asn Ala Ala Val WO 01/00843 WO 0100843PCTIiBOO/00923 gct ctc 1056 Ala Leu ttg gat 1104 Leu Asp ggt cgc 1152 Gly Arg 370 gct cac 1200 Ala His 385 gac ttt 1248 Asp Phe aag gat 1296 Lys Asp att gtg 1344 Ile Val tta gct 1392 Leu Ala 450 caa gaa 1440 Gin Giu 465 gac acc 1488 Asp Thr gca gca Ala Ala 340 325 gtg Val1 gaa gca ttt tcC Glu Ala Phe Phe 345 330 335 ggt gca Ccc gcc gga cgc cca Gly Ala Ser Ala Gly Arg Pro 350 tcC gca caa gtt cag tcc cca Phe Ala Gin Val Gin Ser Pro 365 atc Ile gac acg gtg cgc Asp Thr Val Arg gag cgc ctg cgc Giu Arg Leu Arg gaa Glu 360 ggC Giy tcC gca cca acc gtg tcC atc gac gca Ser Ala Pro Thr Val Phe Ile Asp Ala 380 gca gca CCC ggt gca gca cta gac cgt Ala Ala Leu Gly Ala Ala Leu Asp Arg aac cca cac Asn Pro His gag tcC cgC Glu Phe Arg ggt Gly 390 gcc cgc Ala Arg 420 Cgc acc Cys Thr cgc ctc atc Arg Leu Ile atc Ctg gaa Ile Leu Glu act gcc tca Thr Ala Ser ggC gtc Gly Val 410 395 atc Ile cag Gln 400 gga gtg ctc Cgc gac Gly Val Leu Cys Asp 415 Cca ctt gag cca Cac ctg cat gaa Ser Leu Giu Pro Tyr Leu His Glu 425 430 gag cgc gca Ctg gac gca Cac gaC Glu Arg Ala Leu Asp Ala Tyr Asp 445 Cac ggc gaC gag cgC gtg cac gtc Tyr Gly Asp Giu Arg Val His Val 435 gaa Glu 440 tat gct cga gag Tyr Ala Arg Giu 455 atc I le gat CCC gct ggc Asp Leu Ala Gly 470 gat gta cag tcc Asp Val Gln Ser 485 gcc ggc gaC gcg Ala Gly Asp Ala 500 gcg Ala gta gaa ctc gcC Val Glu Leu Ala 475 att gaa cta gca gaa Ile Giu Leu Ala Giu 480 atc acc ggC Cca atc Ile Thr Gly Ser Ile gga Cca ggC gCC Gly Ser Gly Val 490 cgc acg ctg CCC Arg Thr Leu Phe 505 gtg Val gtg acc 1533 Val Thr gga aag gaa cct Gly Lys Glu Pro 510 495 gca Ala Cgagcaagcg Cgaagaatca atC 1556 WO 01/00843 <210> 698 <211> 511 <212> PRT <213> Corynebacteriui giutamicum <400> 698 Gly Ile Gly Leu Val Ala Asn Asn Asp PCTIBOO/00923 1 Asp Leu Ile Asp Ser Ile Glu Pro Ile 145 Met Ser Ala Asp Gly 225 Gly Thr Ala Ala Ile Ile Gin Arg Met Val1 Leu 115 Leu Pro Met Phe Val1 195 Thr Asp Lys Pro Asn 275 Leu Gly Ala Leu Giu 70 Leu Thr Ala Val1 Phe 150 Ala Leu Ala Ile Asp 230 Ile Asn Leu Glu Asp Gly Leu Thr Met Asn Phe Thr 135 Val Trp Val1 Val1 Asn 215 Arg Ile Val1 Gin Phe Val Glu 40 Arg Lys Gly Gly His 120 Giu Arg Ser Al a Val1 200 Ala Leu Lys Vai Gin 280 G ly Thr Val Leu Ile Gin Lys 105 Arg Arg Ile Giu Leu 185 Giu Ala Gly Aia Ile 265 Ala Val Giy 10 Leu Giu Al a Asp Pro Thr Arg Ile Tyr Ala 170 Ala Val1 Vali Asn Arg 250 Val Val Val Ile Gly Ala Gin Pro 75 Gin Ser Thr Ala Glu 155 Giu Tyr Gly Ser Thr 235 Pro Gly Asp Glu Phe Giu Pro Val Thr Asn Thr Gly Ile 140 Glu Gly Al a Leu Val1 220 Ile Ala Lys Val Ser Asp Thr Ala Giu Phe Ser Thr Arg 125

ASP

Ile Gly Gly Gly 205 Ile Gly Ser Gin Asp 285 Ala Ser Gly Ser Ala Arg Phe Arg 110 Thr Gly Lys Pro Phe 190 Gly Thr Giu Giu Glu 270 Ala Ile Glu Leu Glu Glu Arg Pro Met Thr Lys Pro Lys 175 Ala Arg Pro Ile Asp 255 Pro Ala Ala Asn Ser Giu Leu Met Ala Ile Ser Pro Tyr 160 Met Asp Trp Val Ala 240 Gly Glu Val Val WO 01/00843 290 PCTJIBOO/00923 Gly 305 Ile Ala Leu Gly Al a 385 Asp Lys Ile Leu Gin 465 Asp Val Gly Gin Gin Leu Thr Leu Lys Gly Leu Phe Leu Leu Ala Asp Ile 355 Arg Leu 370 His Asn Phe Giu Asp Ala Val Cys 435 Ala Giu 450 Giu Asp Thr Asp Thr Ala Pro Ala 340 Asp Giu Pro Phe Arg 420 Thr Tyr Leu Val1 Giy 500 Gin 330 Giy Phe Pro Leu Val1 410 Leu Arg Gly Leu Vali 490 Phe Gly 315 Ala Ala Ala Thr Gly 395 Ile Glu Ala Asp Ala 475 Val1 Gly Gly Giu Tyr Thr Asp <210> 699 <211> 600 <212> DNA <213> Corynebacteriun glutanicun <22 0> <221> CDS <222> (577) <223> RXA01517 <400> 699 tccataagcc caaagcaccg atcccacgta cttttgctga cgtcgcggtg gttgcccgac gttccaggaa atccatggct gctggaagga gcaacgccta atg cat gca gtt ttg 115 Met His Ala Val Leu 1 tcc atc ggt tcc aac atg gat gat cgc tac gcg ctg ctc aac aca gtg 163 Ser Ile Gly Ser Asn Met Asp Asp Arg Tyr Ala Leu Leu Asn Thr Val WO 01/00843 atc Ile acc Thr ctc Leu caa Gin cca Pro gag Giu tgg Trp gcc Ala aa a Lys ggC Gly gtt Val1 gaa Giu ga t Asp gag Giu t tc Phe ggc Gly gat Asp att Ile gaa Giu gcc Al a 75 gtg Val1 gat Asp gtg Val1 acg Thr 15 gcg cag tct Ala Gin Ser gat gaa ttc Asp Giu Phe atc gag ttg Ilie Giu Leu gtc cgc gtc Val Arg Vai 80 cag atc att Gin Ile Ile 95 acc ttg cca Thr Leu Pro tgg ttg gaa Trp Leu Giu gaa cat gtg Giu His Val 145 gcg Ala ctc Leu c tg Leu cgc Arg aaa Lys cac His gca Ala 130 gat atc Ile aac Asn cgc Arg aaa Lys gat Asp cc t Pro 115 gaa Giu aa t PCTIIBOO/00923 tac tca 211 Tyr Ser gca gtg 259 Ala Vai cgt ggc 307 Arg Gly tgg ggg 355 Trp Gly ggg gaa 403 Gly Giu 100 tgg gct 451 Trp Ala cct gat 499 Pro Asp ctt gat 547 Asp Asn Leu Asp ccc aca gac att gaa ggt gtc acc Pro Thr Asp Ile Giu Gly Val Thr 150 155 gca <210> 700 <211> 159 <212> PRT <213> Cory-nebacterium giutamicum <400> 700 Met His Ala Val Leu Ser Ile Gly 1 5 Leu Leu Asn Thr Vai Ilie Giu Giu 1 Ser Ala Ilie Tyr Ser Thr Pro Pro 'J 40 Phe Leu Asn Ala Vai Leu Val Val 55 Leu Leu Arg Arg Gly Gin Lys Leu 70 iag att Ile taaggagtcg tggctttcat Asn Met Asp Asp Arg Tyr Ala 10 Lys Asp Giu Ile Vai Aia Gin Gly Ile Giu Asp Gin Asp Giu Val Giu Giu Thr Pro Ile Giu Giu Ala Ala Giu Arg Val Arg 75 s0 WO 01/00843 WO 0100843PCTIIBOOIOO923 Val Arg Lys Trp Gly Pro Arg Thr Leu Asp Val 90 Ile Lys Asp Gly Giu Giu Ilie Leu Ser Giu Asp 100 105 Pro His Pro Trp Ala Trp Gin Arg Ala Phe Vai 115 120 Giu Ala Giu Pro Asp Ala Val Leu His Gly Thr 130 135 Val Asp Asn Leu Asp Pro Thr Asp Ile Giu Gly 145 150 155 <210> 701 <211> 1983 <212> DNA <213> Corynebacteriun glutamicun <22 0> <221> CDS <222> (1960) <223> RXA00579 <400> 701 tcgtctaagt ttttctttga gttttcatat gtagaaggca Asp Ile Val Pro Giu Leu 110 Leu Ile Pro 125 ThrIle Ala 140 Val Thr Lys Gin Ile Thr Leu Trp Leu Giu His Ile tcgtcggctt cggcctggcg gtgcttttct cgttgttttg tggttttgtc agaggatgtc atg cgc gtt tta att Met Arg Val Leu Ile att Ile gag Giu gat Asp gcc Ala gca Ala ttg Leu gag Glu gat Asp gtt Val gag Giu Gly cgc Arg gcc Ala gtt Val aat Asn acg Thr atg Met gtt Val gtt Val1 tat Tyr tcg Ser tat Tyr gg t Gly ctt Leu gcg Ala ccg Pro ggc Gly cag Gin 105 gat Asp cag Gin ttc Phe gct Ala att Ile ggt Gly atc Ile ttc acg Phe Thr cct gtg Pro Val gcc gtc Ala Val 45 ttt ggt Phe Gly 60 ggt gtg Gly Val gtt gat Val Asp cat gat His Asp aat Asn gtg Val ctc Leu tgt Cys tta Leu gcg Al a tca Ser c tc Leu cc t Pro tca Ser gca Ala 9gc G ly 80 ccc Pro ggt Gly 1 gcc Ala aat Asn cc t Pro ggc Gly cac His agg Arg t ta Leu acc Thr gat Asp ggc Gly gtc Val1 cag Gin ccg Pro ttt Phe tat Tyr caa Gin ccg Pro att Ile Gly gtc Val gca Ala 115 gaa Giu ata Ile cac His cgt Arg gcg Ala ggt Gly atc Ile 115 163 211 259 307 355 403 451 WO 01/00843 WO 0100843PCTIEBOO/00923 cct gaa Pro Glu ttg ccg Leu Pro 135 atg gca Met Ala 150 ccg gaa Pro Glu ctt aat Leu Asn ccg ctc Pro Leu tcc tcc Ser Ser 215 ggt gat Gly Asp 230 gag ggg Giu Gly gtt gcg Val Ala tat gag Tyr Giu ctt ccg 1027 Leu Pro 295 tcg gat 1075 Ser Asp 310 gaa gaa 1123 Giu Giu acg Thr 120 gag Giu t tg Leu tct Ser tta Leu agc Ser 200 cat His gcc Ala ga t Asp ccc Pro ctt Leu 280 ga t Asp cag Gin acc Thr ttt Phe tca Ser gca Ala att Ile gcg Ala 185 gtt Val1 gct Ala agc Ser ttc Phe gg t Gly 265 aaa Lys gcg Ala gtt Val atc Ile gag Giu t tg Leu cat His ggt Gly 170 cgc Arg gat Asp ttt Phe ggt Gly ttc Phe 250 caa Gin gcg Ala cac His cgg Arg aag Lys 330 tat Tyr gct Ala ccg Pro ggc Gly tgg Trp 190 gtt Val gat Asp cgc Arg aag Lys ctt Leu 270 gca Ala gcc Ala t tg Leu aat Asn cac His acc Thr cag Gin cat His 175 caa Gin ttt Phe gcc Ala aca Thr gag Giu 255 ggc Gly cgg Arg ga t Asp ggg Gly ctt Leu 335 a tg Met gat Asp 145 gg t Gly atc Ile acg Thr aca Thr gga Gly 225 acc Thr ctc Leu gtt Val1 gcg Ala gcc Ala 305 cag Gin gcc Ala gtg Val 130 gat Asp gtg Val att Ile gag Giu ttc Phe 210 ac c Thr cat His gcc Al a gg t Gly cac His 290 atc Ile gac Asp ccg Pro gca Ala gg t Gly caa Gin aag Lys aaa Lys 195 ttt Phe agc Ser aat Asn gcc Ala tac Tyr 275 act Thr gca Ala gag Giu cgg Arg cgc Arg atc Ile cat His 165 ttc Phe att Ile cat His ctt Leu ggc Gly 245 tca Ser ggt Gly agt Ser gaa Giu ttt Phe 325 cc t Pro 499 547 595 643 691 739 787 835 883 931 979 WO 01/00843 WO 0100843PCT/BOOIOO923 gcg tcc 1171 Ala Ser ctc gac 1219 Leu Asp tat gaa 1267 Tyr Giu 375 cct ctg 1315 Pro Leu 390 ggt gcg 1363 Gly Ala gag cgg 1411 Giu Arg gga cac Gly His 345 aaa att Lys Ile 360 atc tgc Ilie Cys ctc gct ttg cag Leu Ala Leu Gin cgc aga gcc cag Arg Arg Ala Gin 365 ctg acc aca aaa Leu Thr Thr Lys cga gat tcc aaa gat gag tat Arg Asp Ser Lys Asp Glu Tyr 355 ctg att act cgc ggc gaa tcg Leu Ile Thr Arg Giy Giu Ser gag Giu 370 ctt cag ggc acc act Leu Gin Gly Thr Thr 385 gat gtg gcc Asp Val Ala gct gcc tat cta Ala Aia Tyr Leu 380 tat ctt cag Tyr Leu Gin 410 ttc atc acc Phe Ile Thr gca ctg cgt ggg gcc Ala Leu Arg Giy Ala 400 ggg gat acc tct att Gly Asp Thr Ser Ile 415 gat tcg gca ggg tat Asp Ser Ala Gly Tyr aat ccc acc gca tat Asn Pro Thr Ala Tyr 405 ttg agt tcc tcg ccg Leu Ser Ser Ser Pro 420 gtg gaa tca aag ccc Val Glu Ser Lys Pro 435 at t Ile att aaa 1459 Ile LYS atc att 1507 Ile Ilie 455 atg atc 1555 Met Ilie 470 acc aca 1603 Thr Thr gtc cac 1651 Val His ccg att 1699 425 ac c Thr agg Arg ccg cgt ggg Pro Arg Gly 445 430 cga aca gcg Arg Thr Ala caa Gin gag ctg cgc agt Giu Leu Arg Ser 460 aat cct aaa gat cgt Asn Pro LYS Asp Arg 465 aac gac tta gcc cgc Asn Asp Leu Ala Arg gtg gat ttg gtc Val Asp Leu Vai 475 gtt aaa aca tcc Val Lys Thr Ser 490 caa ctt gtc agc Gin Leu Val Ser cgc Arg gaa gac caa gaa Giu Asp Gln Giu 450 gca gaa aac ttg Aia Giu Asn Leu ggc gct ttg ccc Gly Ala Leu Pro 485 acc tac gcc aca Thr Tyr Ala Thr 500 ggg cca cgc agt Gly Pro Arg Ser aag ctt ttc gac Lys Leu Phe Asp 495 acc gtc tct gca Thr Val Ser Ala gag ttg Glu Leu 505 gag tgc 515 ccc ggt ggt tcg atg act ggt gtg cgc gca gca Pro Ilie Giu Cys Val Arg Ala Ala Phe Pro Gly Gly Ser Met Thr Gly WO 01/008 gcc cca 1747 Ala Pro 535 cct cgc 1795 Pro Arg 550 gca gtt 1843 Ala Val cac gtg 1891 His Val 43 aag ctg Lys Leu ggt att Gly Ile gat ctc Asp Leu gag tac Giu Tyr 585 acc Thr tca Ser 555 atg Met gtg Val1 gag Giu Gly arc i Ile I Gly 3 ltc arc gat gag ctg gag Ile Ile Asp Giu Leu Giu 545 :tg gga tat ttt tcc ctc eu Gly Tyr Phe Ser Leu 560 ~ga act ctc gtc atc cag ~rg Thr Leu Val Ile Gin 575 ;ca ctt ctt gct ctg tct la Leu Leu Ala Leu Ser ~90 595 Itt aaa tca cgg cct ctg lal Lys Ser Arg Pro Leu 610 ;tacct cgtgtgggac ggt PCTIB00100923 gca gct Aia Ala gac ggc Asp Gly 565 aac aat Asn Asn 580 gat ccg Asp Pro ctg aat Leu Asn gag gct gag tgg gag gaa atc cgc 1939 Giu Ala Giu Trp Giu Giu Ile Arg 600 605 ttg ttt ggg gtt gaa ttc cca tgac5 1983 Leu Phe Gly Val Giu Phe Pro 615 620 <210> 702 <2i1> 620 <212> PRT <213> Corynebacterium giutamicum <400> 702 Met Arg Vai Leu Ilie Ile Asp Asn 1 5 Ala Thr Tyr Val Giu Giu Val ThrC Asn Asp Gin Glu Ilie Asp Giu Met I Pro Gly Pro Gly His Ala Gly Val 3 Gly Val Ilie Giu Arg Ala Arg Val 70 His Gin Gly Ile Ala Leu Ala Tyr Arg Pro Val His Gly Giu Val Ser 100 Leu Phe Ala Gly Ile Pro Giu ThrI 115 120 ;ly 25 jeu ?ro ;iy ;in ?he Asp 10 Gin Phe Ala Ile Gly 90 Ile Glu Ser Ala Asp Asp Leu Asp Thr Ala Phe Val1 Ile Ile Cys Leu Gly 110 Tyr Asn Val Leu Cys Leu Aia Ser His WO 01/00843 Met Val Ala 130 Asp Asp Gly 145 Gly Val Gin Ilie Ile Lys Thr Giu Lys 195 Thr Phe Phe 210 Gly Thr Ser 225 Thr His Asn Leu Ala Ala Val Gly Tyr 275 Ala His Thr 290 Ala Ile Ala 305 Gin Asp Giu Ala Pro Arg Ser Lys Asp 355 Thr Arg Gly 370 Thr Thr Asp 385 Asn Pro Thr Leu Ser Ser Val Giu Ser 435 Leu Met 150 Pro Leu Pro Ser Gly 230 Giu Val1 Tyr Leu Ser 310 Giu Ala Leu Tyr Pro 390 Gly Giu Ile Pro 135 Ala Glu Asn Leu Ser 215 Asp Gly Ala Giu Pro 295 Asp Glu Ser Asp Glu 375 Leu Aia Arg Lys Glu Leu Ser Leu Ser 200 His Aia Asp Pro Leu 280 Asp Gin Thr Giy Lys 360 Ile Ala Tyr Phe Gly 440 Ser Ala Ile Ala 185 Val Ala Ser Phe Gly 265 Lys Ala Val Ile His 345 Ilie Cys Ala Leu Ile 425 Thr Leu His Gly 170 Arg Asp Phe Gly Phe 250 Gin Ala His Arg Lys 330 Leu Arg Leu Tyr Gin 410 Thr Arg Lys Giu 155 Gly Thr Ser Trp Pro 235 Thr Gly Glu Leu Leu 315 Lys Ala Arg Thr Leu 395 Leu Ile Pro PCTIBOOIOO923 Thr Ser Gin Trp 160 His Gin 175 Gin Leu Phe Glu Ala Gin Thr Lys 240 Giu Asp 255 Gly Trp Arg Ala Asp Arg Gly Giu 320 Leu Val 335 Arg Asp Leu Ile Gin Giy Gly Ala 400 Ser Ile 415 Gly Tyr Thr Ala Gin Giu Asp Gin Gu Ilie Ile Ala Glu Leu Arg Ser Asn Pro Lys Asp WO 01/00843 450 Arg Ala GI 465 Arg Gly A2 Glu Thr T Leu Gly Pi 51 Gly Ser Me 530 Glu Leu GI 545 Phe Ser LE Val Ile GI Ala Leu SE Arg Pro LE 610 <210> 703 <211> 747 PCTIBOO/00923 Asn Leu Ala 500 Arg Thr Ala Asp Asn 580 Asp Leu Leu Pro 485 Thr Ser Gly Al a Gly 565 Asn Pro Asn Met 470 Thr Val Pro Ala Pro 550 Ala His Glu Leu 455 Ile Thr His Ile Pro 535 Arg Val Val Ala Phe 615 Val Val1 Gin Glu 520 Lys Gly Asp Glu Glu 600 Gly Val 475 Ser Ser Arg Thr Ser 555 Met Val Glu Phe 460 Arg

LYS

Thr Ala Met 540 Gly Val Gly Ile Pro 620 Asn Leu Val1 Ala 525 Glu Gly Ile Gly Arg 605 Asp Phe Ser 510 Phe Ile Leu Arg Ala 590 Val Leu

ASP

495 Ala Pro Ile G ly Thr 575 Leu Lys <212> DNA <213> Corynebacterium glutainicum <22 0> <221> CDS <222> (101)..(724) <223> RXA00958 <400> 703 attctaatcc tcaatctgaa gccgatgaga cgttgcacaa ccattgcgct tgctgctggt tccactttgg aggtcatccg ctc att gat aat cac gat tct ttt gtc tac aac Leu Ile Asp Asn His Asp Ser Phe Val Tyr Asn 15 gcc gtg gcc ggt tat aag tgc acg gtg ttc cgc Ala Val Ala Gly Tyr Lys Cys Thr Val Phe Arg 30 gaa acc att ttg gca gcc aac ccg gac ctg atc Glu Thr Ile Leu Ala Ala Asn Pro Asp Leu Ile 45 ggcgtatgcc gtgttgaatg atg aca cac gtt gtt 115 Met Thr His Val Val ctg gtg gat gcg ttc Leu Val Asp Ala Phe aat acg gtg cca gtg Asn Thr Val Pro Val tgc ctt tca cct gga Cys Leu Ser Pro Gly WO 01/00843 WO 0100843PCTIiBOO/00923 cc t Pro aca Thr ctc Leu tac cct gcc gat Tyr Pro Ala Asp ggc aac atg atg Gly Asn Met Met ggc acc acc Gly Thr Thr gt t Val1 cca Pro gtt Val1 150 gg t Gly ctg Leu ttt Phe ggc Gly 135 gcc Ala gat Asp cag Gin ctg Leu aag Lys ctt.

Leu ga t Asp 125 ggc Gly tca Ser cgc Arg gtg Val att Ile gag Giu gat

ASP

gag Giu tat Tyr gg t Gly ac c Thr 175 agc Ser gcg ctg Ala Leu ctc ggc Leu Gly tgt ggc Cys Gly ggt gtg Gly Val gat cat Asp His 130 tca ctg Ser Leu 145 tgt tcc Cys Ser gga aag Gly Lys acg ggt Thr Gly atc gag cgc Ile Giu Arg tac cag gca Tyr Gin Ala cct gtg cac Pro Vai His 100 cag agc cct Gin Ser Pro 115 cca gaa atc Pro Giu Ile ggc tgc gtg Gly Cys Val tcg gag att.

Ser Giu Ile 165 gcc att ggc Ala Ile Gly 180 cct gtc att Pro Val Ilie 195 ttg tcc cgc tgt gtc gaa cag ctt ctc gcg aac taataaaaaa aggatttgz Leu Ser Arg Cys Vai Glu Gin Leu Leu Ala Asn 200 205 tca <210> 704 <211> 208 <212> PRT <213> Corynebacterium giutamicum <400> 704 Met Thr His Val Val Leu Ilie Asp Asn His Asp Ser Phe Val Tyr Asn 1 5 10 Leu Val Asp Ala Phe Ala Val Ala Gly Tyr Lys Cys Thr Val Phe Arg 25 Asn Thr Val Pro Val Giu Thr Ile Leu Ala Ala Asn Pro Asp Leu Ile 40 Cys Leu Ser Pro Gly Pro Gly Tyr Pro Ala Asp Ala Gly Asn Met Met 55 It 744 WO 01/00843 WO 0100843PCTIBOOIOO923 Ala Leu Cys Gly Asp Ser 145 Cys Gly Thr Arg Ala His Pro Ile Val Ile 165 Gly Ile Thr Leu Gly Val1 Pro Val1 150 Gly Leu Leu Leu Lys Leu Asp 125 Gly Ser Arg Val1 Leu 205 Gly Val1 Thr 110 Val1 Arg Leu Thr Leu 190 Leu <210> 705 <211> 1266 <212> DNA <213> Cor-ynebacteriui glutamicum <220> <221> CDS <222> (101)..(1243) <223> RXA02790 <400> 705 tggatgctcg cacccgagcc cttgaaccac aatccacaga agggaaattt cccaggatga accaaatccg aaaccgccgg gta aag cgc cgc caa cgg ttt att gcc Val Lys Arg Arg Gin Arg Phe Ile Ala ctc att atc ggt gcc atc atc tat atc Leu Ile Ile Gly Ala Ile Ile Tyr Ile 30 acg cca cat gac tat gaa ggc tcc gga Thr Pro His Asp Tyr Giu Gly Ser Gly 45 gtg acg Val Thr 15 ggt gta Gly Vai aac ggt Asn Gly cacccaagat ttcgacgaga atg gag ccc gtc tac 115 Met Glu Pro Val Tyr 1 atc gct tca ctc atc 163 Ile Ala Ser Leu Ile gcc acc tca aac cgg 211 Ala Thr Ser Asn Arg gtg gtt cag ctg gtc 259 Val Val Gin Leu Vai WO 01/00843 WO 0100843PCTA~BOO/00923 gaa atc Glu Ile cct gaa ggt tcc Pro Glu Gly Ser ata tca gag ctc Ile Ser Giu Leu cca gag ttg gaa Pro Giu Leu Giu gaa Giu cga gat atc gtg Arg Asp Ile Val acc aac rca gcg Thr Asn Ser Ala r tc Phe caa aca gcg gcc Gin Thr Ala Ala aac aac ccc aac Asn Asn Pro Asn gcg Ala ggt agt gta Cag Giy Ser Val Gin ggt ttc tac cgt Giy Phe Tyr Arg ctg cag Leu Gin 100 gaa caa atg Glu Gin Met aac cag gtt Asn Gin Val 120 gca gca gct gca Ala Ala Ala Ala rcg gct ctg ctt gat cca gac Ser Ala Leu Leu Asp Pro Asp 115 gat ctc ctc gac Asp Leu Leu Asp cac ggc ggc gcc His Gly Gly Ala acc Thr 130 ttg arg gac Leu Met Asp grc act gtt gtc ggc gga aac acc cgc gcg gga Val Thr Val Val Giy Gly Asn Thr Arg Ala Giy 135 140 gca gcc gtg acc tgc acc gaa ggc tcc gcc aac Ala Aia Val Thr Cys Thr Giu Gly Ser Ala Asn arc Ile 145 rac tcc cag atc Tyr Ser Gin Ile tgc atc acc gct Cys Ile Thr Ala gat ttg cag cag gtt gcc tcc acc gtg tcg Asp Leu Gin Gin Val Ala Ser Thr Val Ser 170 175 cct gca gaa rtg ggt gtc Pro Ala Giu Leu Giy Val .180 cca gat tgg Pro Asp Trp cgc ctc gaa Arg Leu Giu 200 arc gct gct gtg Ile Ala Ala Val gct cgc gga act Ala Arg Gly Thr gat cca aag Asp Pro Lys 195 gar cca tcc Asp Pro Ser ggc crg arc atg Gly Leu Ile Met cct Pro 205 ggc caa tac gtg Gly Gin Tyr Val aac gac Asn Asp 215 gcc cag gga arc Ala Gin Giy Ile crc Leu 220 acc gat ctg atc Thr Asp Leu Ile cga. rca gca aac Arg Ser Aia Asn cat His 230 ttc caa gaa acc Phe Gln Giu Thr gac

ASP

235 arc acg ggc cgt Ile Thr Giy Arg gca gat gcc arc gga ctt Ala Asp Ala Ile Gly Leu 240 245 act cca tat gag Thr Pro Tyr Giu gtc acc gca gca Val Thr Ala Ala tct Ser 255 tta atc gag cgc Leu Ile Giu Arg gaa gca Giu Ala 260 cca gca gga Pro Ala Gly gcc gag cca Ala Giu Pro 280 ttt gar aag gtc Phe Asp Lys Vai cgc gtc arc ttg Arg Vai Ile Leu aac cgt crc Asn Arg Leu 275 rac ggr ctg Tyr Giy Leu 931 979 arg cag ctg caa Met Gin Leu Gin ttc Phe 285 gac tcc acc gtc Asp Ser Thr Val WO 01/00843 tct gaa caa gaa gta gca acc acc C 1027 Ser Giu Gin Giu Val Ala Thi- Thi- 1 295 300 cca tgg aac act tac gcc atg gac C 1075 Pro Ti-p Asn Thr Tyr Ala Met AspC 310 315 gca gta tcc acc gaa gca ctc caa 1123 Ala Val Ser Thr Giu Ala Leu Gin 1 330 aac tgg ctg tac ttt gtc acc atc C 1171 Asn Ti-p Leu Tyr Phe Vai Thr Ilie 1 345 aac gac acc ttc gaa gag cac gaa 1219 Asn Asp Thi- Phe Giu Giu His Giu2 360 365 agt ggc gtt cta gac agc aac cga 1266 Ser Giy Val Leu Asp Ser Asn Arg 375 380 <210> 706 <211> 381 <212> PRT <213> Corynebacterium giutamicum <400> 706 Met Giu Pro Vai Tyr Vai Lys Arg J 1 Ile Ala Ser Leu Ile Leu Ile Ile( Ala Thr Ser Asn Arg Thi- Pro His J 40 Val Val Gin Leu Val Giu Ilie Pro( 55 Gly Pro Giu Leu Giu Giu Arg Asp Gin Thr Ala Ala Ser Asn Asn Pro 2 Phe Tyr Arg Leu Gin Giu Gin Met 100 Leu Leu Asp Pro Asp Asn Gin Val 2 115 120 PCT/EBOO/00923 ~ac gaa gac cgt cag acc gtc acc ~sp Giu Asp Arg Gin Thr Val Thr 305 ;gc ctg cca caa acc ccc atc gcc ;iy Leu Pro Gin Thr Pro Ile Ala 320 325 ;cc atg gaa aac cct gca gaa gga l.a Met Giu Asn Pro Ala Giu Giy 335 340 jac acc gat gga acc acc gtg ttc ~sp Thr Asp Gly Thr Thr Val Phe 50 355 Icc gac att gag caa gct ttg aac kla Asp Ile Giu Gin Ala Leu Asn 370 taaggatcag cgaataaaat tgg Phe Ile Gly Ser Thr Ser Ala Asp Ile Tyr Ser Ile Asn Val Ala Ile 125 WO 01/00843 PCTLBOO/00923 Ala Thr Leu Met Asp Val Thr Val Vai Gly Giy Asn Thr Arg Ala Gly 130 135 140 Ile Tyr Ser Gln Ilie Ala Ala Val Thr Cys Thr Glu Gly Ser Ala Asn 145 150 155 160 Cys Ile Thr Ala Giu Asp Leu Gin Gin Val Ala Ser Thr Val Ser Pro 165 170 175 Ala Giu Leu Giy Val Pro Asp Trp Ala Ile Ala Ala Val Giu Ala Arg 180 185 190 Gly Thr Asp Pro Lys Arg Leu Giu Gly Leu Ile Met Pro Gly Gin Tyr 195 200 205 Val Val Asp Pro Ser Asn Asp Ala Gin Gly Ile Leu Thr Asp Leu Ile 210 215 220 Thr Arg Ser Ala Asn H-is Phe Gin Giu Thr Asp Ile Thr Gly Arg Ala 225 230 235 240 Asp Ala Ilie Gly Leu Thr Pro Tyr Giu Leu Val Thr Ala Ala Ser Leu 245 250 255 Ilie Glu Arg Giu Ala Pro Ala Gly Asp Phe Asp Lys Val Ala Arg Val 260 265 270 Ilie Leu Asn Arg Leu Ala Giu Pro Met Gin Leu Gin Phe Asp Ser Thr 275 280 285 Val Asn Tyr Gly Leu Ser Glu Gin Giu Val Ala Thr Thr Asp Giu Asp 290 295 300 Arg Gin Thr Val Thr Pro Trp Asn Thr Tyr Ala Met Asp Gly Leu Pro 305 310 315 320 Gin Thr Pro Ile Ala Ala Val Ser Thr Giu Ala Leu Gin Ala Met Giu 325 330 335 Asn Pro Ala Giu Gly Asn Trp Leu Tyr Phe Val Thr Ile Asp Thr Asp 340 345 350 Gly Thr Thr Val Phe Asn Asp Thr Phe Giu Giu His Giu Ala Asp Ile 355 360 365 Giu Gin Ala Leu Asn Ser Gly Val Leu Asp Ser Asn Arg 370 375 380 <210> 707 <211> 579 <212> DNA <213> Corynebacterium giutaxnicum <22 0> <221> CDS <222> (556) <223> RXA00106 <400> 707 tggagctcaa caaggcagcg tacatgtttg agtacagctt cgatgacatc accgtgtccg WO 01/00843 WO 0100843PCTIBOOIOO923 gctacgatcc acacccattg atccgcggca aggtcgccgt atg atc ggt Met Ile Giy tgg Trp tgg Trp cag Gin ccg Pro gac

ASP

tgg Trp, gac Asp ccc Pro tcc Ser atc Ile 150 gca Al a cac His ccg Pro ctt Leu tgg Trp atc Ile gtt Val gtc Val gag Glu 135 aag Lys caa Gin atc Ile gtc Val ccc Pro tcc Ser atg Met tta Leu tac Tyr 120 tgg Trp gt t Val1 ggc Gly ccg Pro a tc Ile Giy gcc Ala ggc Gly gaa Glu 105 gca Aia t tt cgt Arg gaa Glu atg Met cgc Arg ggc Giy Giy ata Ile ccc Pro acc gac Asp gac Asp gg t Gly gag Glu ggc Gly 75 ggC Gly acg Thr gaa Glu tca ggc Giy ctc Leu cgt Arg aac Asn 60 aca Thr gag Giu ctt Leu atc Ile ggc G iy 140 atc Ile aaa Lys cgc Arg 45 t tc Phe gtg Val1 gtc Val1 atc Ile ccg Pro 125 gag ggc Giy 15 ttc Phe tgg Trp ctc Leu acc Thr aag Lys 95 gcc Ala aac Asn cgt gac Asp aag Lys gag Giu tcc Ser gaa Giu 80 gcc Ala acc Thr ttc Phe tac Tyr ggc Giy aaa Lys tct Ser tca Ser atc Ile acc Thr ttc Phe aac Asn aag Lys 145 acc Thr acc Thr t tg Leu cgc Arg cct Pro gtc Val gat Asp ctc Leu 130 t tc Phe gac Asp acc Thr ccg Pro gag Giu aaa Lys ggc G ly gtt Val 115 gat Asp cag Gin gcg Ala atg Met atg Met t tc Phe ccc Pro agc Ser agc Ser 100 t cc Ser gac Asp cgc Arg att Ile ccc Pro ggc Gly aag Lys ggc Gly ggc Gly gcc Ala act Thr gaa Giu tac Tyr 115 163 211 259 307 355 403 451 499 547 Phe Thr Ser Giu Tyr Arg taaggagcaa acaacatgag caa <210> 708 <211> 152 <212> PRT <213> Corynebacteriun giutanicum <400> 708 Met Ile Gly Ala Ile Trp Ala Gin Gly Arg Asp Gly Ile Ile Gly Asp 1 5 10 Giy Thr Asp Met Pro Trp His Ile Pro Glu Asp Leu Lys Hius Phe Lys 25 Lys Thr Thr Met Gly Gin Pro Val Ile Met Gly Arg Arg Thr Trp Giu 40 WO 01/00843 Ser Leu Pro Phe Lys Pro Leu Pro Gly Arg Glu 55 Ser Arg Glu Pro Gly Asp Trp, Ser Ala Gly Gly 70 75 Ilie Pro Lys Ser Gly Trp, Ile Met Gly Gly Gly 90 Thr Val Gly Ser Ala Asp Val Leu Glu Ile Thr 100 105 Phe Asp Val Ser Thr Pro Val Tyr Ala Pro Glu 115 120 Asn Leu Asp Asp Glu Ser Giu Trp, Phe Thr Ser 130 135 Lys Phe Gin Arg Tyr Ilie Lys Val 145 150 <210> 709 <211> 2599 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (101)..(2599) <223> RXN02198 <400> 709 agactagtgg cgctttgcct gtgttgctta ggcggcgttg agttcgggaa ttgtctaatc cgtactaagc tgtctacaca Asn Thr Glu Leu Ile Gly 140 PCTJIBOO/00923 Leu Ser Thr Glu Lys Ala Ala Thr Asn Phe Arg Tyr act Thr ttg Leu caa Gin tgt Cys cac His ttt Phe gcc Al a cat His gac Asp at t Ile tac Tyr aac Asn cac His gtg Val1 ctg Leu ctc Leu ttt Phe ctg Leu cat His gac Asp 30 aag Lys cgc Arg gc t Ala gcg Al a 987 tcc Ser gcc Ala t tc Phe gat Asp ttg Leu 80 tat Tyr aaaatgaact acgaatgaaa atg tct act tca gtt Met Ser Thr Ser Val 1 gaa ttt ttg gat gcg Giu Phe Leu Asp Ala atg ggc acc cag ctc Met Gly Thr Gin Leu ctt gat ctg gag ggg Leu Asp Leu Glu Gly gtg ttg agg cag att Val Leu Arg Gin Ile gtt gag acc aat act Val Giu Thr Asn Thr gac atc gct gat cgt Asp Ile Ala Asp Arg WO 01/00843 WO 0100843PCT[IBOO/00923 tgc cgt gag ctt gcc tac aag ggc Cys Arg Giu Leu Ala Tyr Lys Gly 105 gca gtg gct agg gaa gtg gct Ala Val Ala Arg Glu Val Ala 115 gat gag atg Asp Giu Met 120 ggg ccg ggc cga Gly Pro Giy Arg ggc atg cgg cgt Gly Met Arg Arg t tc Phe 130 gtg gtt ggt Val Val Gly tcc ctg Ser Ldu 135 gga cct gga acg Gly Pro Giy Thr ctt cca tcg ctg Leu Pro Ser Leu cat gca ccg tat His Ala Pro Tyr gca Ala 150 gat ttg cgt ggg cac tac aag gaa gca Asp Leu Arg Gly His Tyr Lys Glu Ala 155 ctt ggc atc atc Leu Giy Ile Ile ggt ggt ggc gat Giy Gly Giy Asp gcc Ala 170 ttt ttg att gag Phe Leu Ile Glu gct cag gac ttg Ala Gin Asp Leu ctt cag Leu Gin 180 gtc aag gct Val Lys Ala aca ttc ttg Thr Phe Leu 200 gtt cac ggc gtt Vai His Gly Val gat gcc atg gct Asp Ala Met Ala gaa ctt gat Giu Leu Asp 195 acc ggc acc Thr Gly Thr ccc att att tgc Pro Ile Ile Cys gtc acc gta gag Val Thr Val Glu acc Thr 210 atg ctc Met Leu 215 atg ggt tct gag Met Gly Ser Giu ggt gcc gcg ttg Giy Ala Ala Leu gcg ctg cag cca Ala Leu Gin Pro ggt atc gac atg Gly Ile Asp Met att Ilie 235 ggt ctg aac tgc Gly Leu Asn Cys acc ggc cca gat Thr Gly Pro Asp atg agc gag cac Met Ser Giu His ctg Leu 250 cgt tac ctg tcc Arg Tyr Leu Ser cac gcc gat att His Ala Asp Ile cct gtg Pro Val 260 tcg gtg atg Ser Val Met gaa tac cca Giu Tyr Pro 280 aac gca ggt ctt Asn Aia Gly Leu gtc ctg ggt aaa Vai Leu Gly Lys aac ggt gca Asn Gly Ala 275 gct gga ttc Ala Giy Phe ctt gag gct gag Leu Glu Ala Giu gat Asp 285 ttg gcg cag gcg Leu Ala Gin Ala gtc tcc 1027 Val Ser 295 cct gag 1075 Pro Giu 310 gaa tat ggc ctg tcc atg gtg ggt ggt tgt tgt ggc acc aca Giu Tyr Gly Leu Met Val Gly Gly Cys Gly Thr Thr cac atc cgt gcg gtc cgc gat gcg gtg gtt ggt gtt cca gag His Ile Arg Ala 315 Val Arg Asp Ala Val Gly Val Pro WO 01/00843 WO 0100843PCTIiBOO/00923 cag gaa 1123 Gin Glu gcc tcc 1171 Ala Ser tcg gtg 1219 Ser Val acc aac 1267 Thr Asn 375 gat tgg 1315 Asp Trp 390 gca cac 1363 Ala His gcc gat 1411 Ala Asp cca atc 1459 Pro Ile gag cac 1507 Giu His 455 ggc gat 1555 Gly Asp 470 cag cac 1603 Gin His gca cgt 1651 Ala Arg acc tcc aca Thr Ser Thr 330 cgc gag gtg Arg Glu Val ctg acc aag atc cct Leu Thr Lys Ile Pro 335 gag aaa gag gac tcc Glu Lys Giu Asp Ser 350 cag gaa acc ggc att Gin Glu Thr Giy Ile gca ggc cct gtt gag cag Ala Gly Pro Val Giu Gin 340 gtc gcg tcg ctg tac acc Val Ala Ser Leu Tyr Thr 355 tcc atg atc ggt gag cgc Ser Met Ile Gly Giu Arg 345 cca ttg Pro Leu tcc Ser 365 tcc aac ggt tcc Ser Asn Gly Ser gaa aag tgt gtg Glu Lys Cys Val aag Lys 380 gca Ala ttc cgt gag gca Phe Arg Giu Ala 385 atg ctg gat Met Leu Asp 410 atg gcg acc Met Ala Thr gat att gcc aag cag Asp Ile Ala Lys Gin 400 tgt gtg gat tac gtg Cys Val Asp Tyr Val 415 gca gca ctt ctt gct Ala Ala Leu Leu Ala caa Gin gga Glv 370 atg ctg tct ggc Met Leu Ser Gly acc cgc gat ggt Thr Arg Asp Gly 405 cga gac ggc acc t tg Leu 420 acc agc tcc act ttg Thr Ser Ser Thr Leu 435 425 atg att Met Ile 430 gac tcc acc gag Asp Ser Thr Glu 445 440 t tg Leu cca Pro gtt Val gag gtt att cgc aca ggc ctt Glu Val Ile Arg Thr Gly Leu 450 aac tcc gtc aac ttt gaa gac Asn Ser Val Asn Phe Glu Asp ggt gga cga agc Gly Gly Arg Ser 460 a tc Ile 465 ggc cct gag tcc Gly Pro Glu Ser 475 ggt gcg gcc gtg Gly Ala Ala Val 490 acc gct gag cac Thr Ala Giu His 505 cgc Arg tac cag cgc atc Tyr Gin Arg Ile 480 atg aaa Met Lys ctg gta aag Leu Val Lys 485 gtt gcg ctg acc Val Ala Leu Thr 495 aag gtg cgc att Lys Val Arg Ile 510 att gat gag gaa ggc cag Ile Asp Giu Glu Gly Gin 500 gct aaa cga ctg att gac Ala Lys Arg Leu Ile Asp 515 WO 01/00843 WO 0100843PCTIBOO/00923 gat atc 1699 Asp Ile tgC ctg 1747 Cys Leu 535 ggc att 1795 Gly Ile 550 gaa atc 1843 Glu Ilie cct gct 1891 Pro Ala gag gct 1939 Giu Ala atg aac 1987 Met Asn 615 tat gat 2035 Tyr Asp 630 ctg ttt 2083 Leu Phe cag ctg 2131 Gin Leu gac ggc 2179 Asp Gly gag aag 2227 Giu Lys 695 ggc agc tac ggc ctg Gly Ser Tyr Gly Leu 525 ttc ccg atc tct act Phe Pro-Ilie Ser Thr gat atc aaa gac atc gtt gtg gac Asp Ile Lys Asp Ile Val Val Asp 530 ggc cag gaa gaa acc agg cga gat Gly Gin Giu Giu Thr Arg Arg Asp 545 cgc gag ctg aag aag ctc tac cca Arg Giu Leu Lys Lys Leu Tyr Pro 560 565 gaa acc atc gaa Giu Thr Ile Giu 555 cac acc acc ctg His Thr Thr Leu 570 gca cgc cag gtt Ala Arg Gin Val atc Ile ggt ctg tcc aat Gly Leu Ser Asn 575 ctt aac tct gtg Leu Asn Ser Val att tcc ttc ggc ctg aac Ile Ser Phe Giy Leu.Asn 580 ttc ctc aat gag tgc att Phe Leu Asn Giu Cys Ile 595 585 ggt ctg Glv Leu 590 gac tct gcg att Asp Ser Ala Ilie 605 gcg cac agc Aia His Ser cgc gaa gtg Arg Giu Vai tcc aag att ttg ccg Ser Lys Ile Leu Pro 610 gcg ttg gat atg gtc Ala Leu Asp Met Vai att gat gat cgc Ile Asp Asp Arg 620 cag Gin cgc cgc acc gag Arg Arg Thr Giu 635 gag ggc gtt tct Giu Giy Vai Ser 650 gcc gct atg cct Ala Ala Met Pro gat tac gat ccg ctg Asp Tyr Asp Pro Leu 640 gct gcc gat gcc aag Ala Ala Asp Ala Lys 655 625 cag Gin ga t Asp gaa ttc atg cag Giu Phe Met Gin 645 gct cgc gct gaa Ala Arg Ala Giu 660 ttg ttt gag Leu Phe Giu cgt ttg gca cag cgc atc atc Arg Leu Ala Gin Arg Ile Ile 675 665 gat aag Asp Lys aat ggc ctt Asn Gly Lieu 670 gag gat gat ctg gaa gca ggc atg aag Giu Asp Asp Leu Giu Aia Gly Met Lys 685 690 atc aac gag gac ctt ctc aac ggc atg Ile Asn Giu Asp Leu Leu Asn Giy Met 705 cct att gcg atc Pro Ile Aia Ile 700 WO 01/00843 WO 0100843PCT/IBOO/00923 aag acc 2275 gtg ggt gag ctg ttt ggt tcc gga cag atg cag ctg cca ttc Lys Thr Val Gly Glu Leu Phe Gly Ser Gly Gin Met Gin Leu Pro Phe 710 gtg ctg 2323 Val Leu ccg ttc 2372.

Pro Phe ggc aag 2419 Gly Lys atc ggc 2467 Ile Gly 775 gtg gtg 2515 Val Val 790 gcg gaa 2563 Ala Giu aag tcc 2599 Lys Ser caa Gin atg Met ggc Gly 760 aag Lys aac Asn gaa Glu acc Thr tcg Ser gaa Glu 745 aaa Lys aac Asn t tg Leu cac His gtg Val 825 gca Aia 730 gag Glu atc Ile ttg Leu 9gc Giy a aa

LYS

810 gtg Val 715 gaa Giu gaa Glu gtc Vai gtg Val1 atc Ile 795 gca Ala atg Met a tg Met gaa Giu gcc Ala 765 atc Ile cag Gin gtC Vali caa Gin act Thr 735 ac c Thr gtc Val t tg Leu ctg Leu ggc Giy 815 atc Ile 720 gcg Ala gga Gly aag Lys tcc Ser tcc Ser 800 a tg Met agc Ser gtg Val tct Ser ggt Giy aac Asn 785 gcc Ala tcg Ser gac Asp 725 gaa Glu gag Glu ga t Asp gac Asp gca Aia 805 g tg Val1 <210> 710 <211> 833 <212> PRT <213> Corynebacterium glutamicum <400> 710 Met Ser Thr Ser Val Thr Ser Pro Ala 1 5 Giu Phe Leu Asp Ala Leu Ala Asn His 25 Met Gly Thr Gin Leu Gin Gly Phe Asp 40 Leu Asp Leu Giu Gly Cys Asn Giu Ile 55 Vai Leu Arg Gin Ile His Arg Ala Tyr 70 Asn Ala His Ser Ser Ile Gly Asp Gly Ala Val Giu Lys Asp Phe Asp Thr Arg Pro Asp Ala Gly Ala Asp Leu WO 01/00843 WO 0100843PCTIBOO/00923 Val Asp Ala Arg Gly 145 Leu Gin Met Giu Thr 225 Thr Ala Gly Ala Cys 305 Val Gly Ala Met Ala 385 Thr Asn Thr Ala Asp Arg 100 Giu Val Ala 115 Val Val Gly Ala Pro Tyr Ile Ile Asp 165 Leu Leu Gin 180 Giu Leu Asp 195 Thr Gly Thr Leu Gin Pro Pro Asp Glu 245 Ile Pro Val 260 Asn Gly Ala 275 Ala Gly Phe Gly Thr Thr Val Pro Glu 325 Val Giu Gin 340 Leu Tyr Thr 355 Gly Glu Arg Leu Ser Gly Phe Gly Cys Asn Leu Pro Asn Leu Ala Asp Tyr 90 Cys Asp Ser Al a 150 Gly Val1 Thr Met Leu 230 Met Ser Giu Val Pro 310 Gin Ala Ser Thr Asp 390 Arg Giu Leu 135 Asp Gly Lys Phe Leu 215 Gly Ser Val1 Tyr Se r 295 Glu Giu Ser Val1 Asn 375 Trp Giu Met 120 Gly Leu Gly Ala Leu 200 Met Ile Giu Met Pro 280 Glu His Thr Arg Pro 360 Ser Glu Tyr Gly Thr His 155 Phe His Ile Giu Ile 235 Arg Ala Ala Leu Ala 315 Leu Glu Gin Ser Val 395 Lys Arg Lys 140 Tyr Leu Gly Cys Ile 220 Gly Tyr Gly Giu Ser 300 Val Thr Lys Giu Lys 380 Asp Thr 110 Gly Pro Giu Giu Gin 190 Val1 Ala Asn Ser Pro 270 Leu Val Asp Ile Asp 350 Gly Phe Al a Ala Met Ser Ala Thr 175 Asp Thr Ala Cys Lys 255 Val1 Ala Gly Ala Pro 335 Ser Ile Arg Lys Val1 Arg Leu Al a 160 Ala Ala Val Leu Ala 240 His Leu Gin Gly Val 320 Ala Val1 Ser Giu Gin 400 WO 01/00843 Gin Thr Arg Giy Arg Asp Thr Ser Ser 435 Ile Arg Thr 450 Val Asn Phe 465 Met Lys Leu Asp Glu Glu Lys Arg Leu 515 Asp Ile Vai 530 Glu Thr Arg 545 Lys Lys Leu Ser Phe Gly Leu Asn Glu 595 Ser Lys Ilie 610 Ala Leu Asp 625 Gin Giu Phe Asp Aia Arg Aia Gin Arg 675 Giu Ala Giy 690 Leu Leu Asn 705 Gly 405 Thr Leu Leu Asp Lys 485 Gin Asp Asp Asp Pro 565 Asn Ile Pro Val Gin 645 Giu Ile Lys Met Aila Aila Pro Giu Giy 470 Gin Aia Asp Cys Gly 550 Glu Pro Giu Met Tyr 630 Leu Gin Asp Giu Lys 710 His Asp Ile His 455 Asp His Arg Ile Leu 535 Ile Ile Ala Ala Asn 615 Asp Phe Leu Gly Lys 695 Thr Met Met Met 440 Leu Gly Giy Thr Thr 520 Thr Giu His Ala Giy 600 Arg Arg Giu Ala Asp 680 Ser Val Leu Asp 410 Ala Thr 425 Ile Asp Giy Giy Pro Giu Ala Ala 490 Ala Giu 505 Gly Ser Phe Pro Thr Ile Thr Thr 570 Arg Gin 585 Leu Asp Ile Asp Arg Thr Giy Val 650 Ala Met 665 Lys Asn Pro Ile Gly Giu Leu Leu Ser Arg Ser 475 Val His Tyr Ile Giu 555 Leu Val Ser Asp Giu 635 Ser Pro Giy Ala Leu 715 Cys Al a Thr Ser 460 Arg Val1 Lys Gly Ser 540 Ala Gly Leu Ala Arg 620 Asp Ala Leu Leu Ile 700 Phe Thr Val Ala Giu 445 Ile Tyr Ala Val1 Leu 525 Thr Ile Leu Asn Ile 605 Gin Tyr Ala Phe Giu 685 Ile Gly Met Asp Leu 430 Pro Vai Gin Leu Arg 510 Asp Gly Arg Ser Ser 590 Ala Arg Asp Asp Giu 670 Asp Asn Ser Lys PCT/IBOO/00923 Tyr Val 415 Leu Ala Giu Val Asn Ser Arg Ile 480 Thr Ile 495 Ile Ala Ile Lys Gin Giu Giu Leu 560 Asn Ile 575 Val Phe His Ser Giu Val Pro Leu 640 Ala Lys 655 A rg Leu Asp Leu Giu Asp Giy Gin 720 Thr Ala Met Gin Leu Pro Phe Val Leu Gin Ser Ala Giu WO 01/00843 Val Ser Gly Asn 785 Ala Ser Asp Leu 740 Ala His Tyr Glu Leu 820 Glu Glu Asp Asp Ala 805 Val1 Giu 745 Lys Asn Leu His Val 825 730 Glu Ile Leu Gly Lys 810 Val Ala 750 Thr Ile Pro Ile Thr 830 PCTTBOO/00923 735 Thr Gly Val Lys Leu Ser Leu Ser 800 Gly Met 815 Ile Ser <210> 711 <211> 2578 <212> DNA <213> Corynebacteriui glutamicum <220> <221> CDS <222> (101)..(2578) <223> FRXA02198 <400> 711 agactagtgg cgctttgcct gtgttgctta ggcggcgttg agttcgggaa ttgtctaatc cgtactaagc tgtctacaca act Thr ttg Leu caa Gin tgt Cys cac His tca Ser gca Ala ggc Gly aat Asn cgc Arg aac Asn t tg Leu gac Asp aac Asn gag Glu 75 gca cat Ala His ggc gac Gly Asp gaa aag Giu Lys acc cgc Thr Arg gga gct Giy Ala tcc Ser Gly gat Asp cct Pro gac Asp tcc Ser gcc Al a ttc Phe gat Asp t tg Leu 80 aaaatgaact acgaatgaaa atg tct act tca gtt Met Ser Thr Ser Val 1 gaa ttt ttg gat gcg Glu Phe Leu Asp Ala atg-ggc acc cag ctc Met Gly Thr Gin Leu Ctt gat ctg gag ggg Leu Asp Leu Glu Gly gtg ttg agg cag att Val Leu Arg Gin Ile gtt gag acc aat act Val Giu Thr Asn Thr 115 163 211 259 307 355 WO 01/00843 WO 0100843PCTIIBOO/00923 ttt ggt Phe Gly tgc cgt Cys Arg gat gag Asp Glu tcc ctg Ser Leu 135 gca gat Ala Asp 150 ggt ggt Gly Gly gtc aag Val Lys aca ttc Thr Phe atg ctc Met Leu 215 ctg ggt Leu Gly 230 atg agc Met Ser tcg gtg Ser Val gaa tac Glu Tyr gtc tcc 1027 Val Ser 295 cct gag 1075 Pro Glu 310 tgc aac ctg ccg aac ttg gcg gat tat gac Cys Asn Leu Pro Asn Leu Ala ASP Tyr ASP 95 gag Glu atg Met 120 gga Gly t tg Leu ggc Gly gc t Al a ttg Leu 200 atg Met atc Ile gag Glu atg Met cca Pro 280 gaa Glu cac His ctt Leu 105 ggg Gly cc t Pro cgt Arg gat Asp gcg Ala 185 ccc Pro ggt Gly gac

ASP

cac His cct Pro 265 ctt Leu tat Tyr atc Ile gcc Ala ccg Pro gga Gly ggg Gly gc c Ala 170 gt t Val1 at t Ile tct Ser atg Met ctg Leu 250 aac Asn gag Glu ggc Gly cgt Arg tac Tyr ggc, Giy acg Thr cac His 155 t tt Phe cac His att Ile gag Glu att Ile 235 cgt Arg gca Ala gc t Al a ctg Leu gcg Ala 315 aag Lys cga Arg aag Lys 140 tac Tyr ttg Leu ggc Gly tgc Cys atc Ile 220 ggt Gly tac Tyr ggt Gly gag Glu tcc Ser 300 gtc Val ggc Gly aac Asn 125 ctt Leu aag Lys att Ile gtt Val cac His 205 ggt G ly ctg Leu c tg Leu ct t Leu gat Asp 285 atg Met cgc Arg act Thr 110 ggc Gly cca Pro gaa Glu gag Glu c aa Gin 190 gtc Val gcc Ala aac Asn tcc Ser cct Pro 270 t tg Leu gtg Vai gat Asp gca Ala atg Met tcg Ser gca Ala act Thr 175 gat Asp acc Thr gcg Ala tgc Cys aag Lys 255 gtc Val1 gcg Ala ggt Gly gcg gtg Val cgg Arg ctg Leu gcg Ala 160 gc t Ala gcc Al a gta Vai ttg Leu gcc Ala 240 cac His ctg Leu cag Gin ggt Gly gtg gct Ala cgt Arg ggc Gly 145 ctt Leu cag Gin atg Met gag Glu aca Thr 225 acc Thr gcc Al a gg t Gly gcg Ala tgt Cys 305 atc gct gat Ile Ala Asp 100 agg gaa gtg Arg Giu Val 115 ttc gtg gtt Phe Val Val 130 cat gca ccg His Ala Pro ggc atc atc Gly Ile Ile gac ttg ctt Asp Leu Leu 180 gct gaa ctt Ala Glu Leu 195 acc acc ggc Thr Thr Gly 210 gcg ctg cag Ala Leu Gin ggc cca gat Gly Pro Asp gat att cct Asp Ile Pro 260 aaa aac ggt Lys Asn Gly 275 ctg gct gga Leu Ala Gly 290 tgt ggc acc Cys Gly Thr cgt Arg gc t Ala ggt Gly tat Tyr gac

ASP

165 cag Gin gat Asp acc Thr cca Pro gag Glu 245 gtg Vai gca Ala t tc Phe aca Thr gtt ggt gtt cca gag Val Gly Val Pro Glu 325 Ala Val 320 WO 01/00843 WO 0100843PCTIBOO/00923 cag gaa 1123 Gin Giu gcc tcc 1171 Ala Ser tcg gtg 1219 Ser Val acc tcc aca Thr Ser Thr 330 cgc gag gtg Arg Giu Val ctg acc aag atc cct Leu Thr Lys Ile Pro 335 gag aaa gag gac tcc Giu Lys Giu Asp Ser 350 cag gaa acc ggc att Gin Giu Thr Gly Ile gca ggc cct gtt gag cag Ala Gly Pro Vai Giu Gin 340 gtc gcg tcg ctg tac acc Val Ala Ser Leu Tyr Thr 355 tcc atg atc ggt gag cgc Ser Met Ile Gly Giu Arg 370 345 cca ttg Pro Leu tcc Ser 360 365 acc aac tcc 1267 Thr Asn Ser 375 gat tgg gaa 1315 Asp Trp Giu 390 gca cac atg 1363 Ala His Met aac gg~t tcc Asn Gly Ser aag tgt gtg Lys Cys Vai aag Lys 380 gca Al a ttc cgt gag gca atg ctg tct ggc Phe Arg Glu Aia Met Leu Ser Gly 385 gat att gcc aag cag Asp Ile Aia Lys Gin 400 caa acc cgc gat ggt Gin Thr Arg Asp Gly 405 ctg gat Leu Asp 410 gcg acc Ala Thr 395 ctt Leu tgt gtg Cys Val gcc gat 1411 Aia Asp cca atc 1459 Pro Ile gag cac 1507 Giu His 455 ggc gat 1555 Gly Asp 470 cag cac 1603 Gin His gca cgt 1651 Ala Arg atg Met ttg gca gca Leu Ala Ala 425 atg att Met Ile gac tcc acc gag Asp Ser Thr Giu 445 gat tac gtg gga cga gac ggc acc Asp Tyr Val Giy Arg Asp Gly Thr 415 420 ctt ctt gct acc agc tcc act ttg Leu Leu Ala Thr Ser Ser Thr Leu 430 435 cca gag gtt att cgc aca ggc ctt Pro Giu Vai Ile Arg Thr Gly Leu 450 gtt aac tcc gtc aac ttt gaa gac Val Asn Ser Val Asn Phe Glu Asp 465 cag cgc atc atg aaa ctg gta aag Gin Arg Ile Met Lys Leu Val Lys 480 485 440 ttg Leu ggt gga cga agc Gly Giy Arg Ser 460 atc Ile tac Tyr ggc cct gag tcc Gly Pro Giu Ser 475 cgc Arg ggt gcg Giy Ala acc gct Thr Ala 505 gcc gtg gtt gcg ctg acc Aia Val Vai Ala Leu Thr 490 495 gag cac aag gtg cgc att Giu His Lys Val Arg Ile 510 att gat gag gaa ggc cag Ile Asp Giu Giu Gly Gin 500 gct aaa cga ctg att gac Ala Lys Arg Leu Ile Asp 515 WO 01/00843 WO 0100843PCTIBOO/00923 gat atc 1699 Asp Ilie tgC ctg 1747 CyS Leu 535 ggc att 1795 Gly Ile 550 gaa atc 1843 Giu Ile cct gct 1891 Pro Ala gag gct 1939 Giu Ala atg aac 1987 Met Asn 615 tat gat 2035 Tyr Asp 630 ctg ttt 2083 Leu Phe cag ctg 2131 Gin Leu gac ggc 2179 Asp Gly acc Thr 520 ggc agc tac ggc ctg gat Gly Ser Tyr Giy Leu Asp 525 atc aaa gac atc gtt gtg gac Ile Lys Asp Ile Val Val Asp 530 cag gaa gaa acc agg cga gat Gin Glu Giu Thr Arg Arg Asp acc ttc ccg atc tct Thr Phe Pro Ile Ser 540 gaa acc atc gaa gcc Giu Thr Ile Glu Ala 555 cac acc acc ctg ggt His Thr Thr Leu Gly 570 gca cgc cag gtt ctt Aia Arg Gin Val Leu act ggc Thr Gly atc cgc gag ctg Ile Arg Giu Leu 560 ctg tcc aat att Leu Ser Asn Ile 575 aac tct gtg ttc Asn Ser Val Phe 545 aag

LYS

aag ctc tac cca Lys Leu Tyr Pro 565 tcc ttc ggc ctg aac Ser Phe Gly Leu Asn 580 ctc aat gag tgc att Leu Asn Giu Cys Ile 595 585 ggt ctg Gly Leu gac tct gcg att Asp Ser Aia Ile 605 gat gat cgc cag Asp Asn Arci Gin 590 600 cgc Arg gcg cac agc tcc aag att ttg ccg Ala His Ser Ser Lys Ile Leu Pro 610 cgc gaa gtg gcg, ttg gat atg gtc Arg Giu Val Ala Leu Asp Met Val at t 620 cgc cgc acc gag Arg Arg Thr Giu 635 gag ggc gtt tct Glu Giy Vai Ser 650 gat tac gat ccg Asp Tyr Asp Pro gct gcc gat gcc Ala Aia Asp Ala 655 ttg ttt gag cgt Leu Phe Giu Arg ctg Leu 640 625 cag Gin gaa ttc atg cag Giu Phe met Gin 645 gcc gct Aia Ala 665 atg cct Met Pro aag gat gct cgc gct gaa Lys Asp Ala Arg Ala Glu 660 ttg gca cag cgc atc atc Leu Ala Gin Arg Ilie Ilie 675 ctg gaa gca ggc atg aag Leu Giu Ala Gly Met Lys 670 ga t Asp 680 gag aag tct 2227 Giu Lys Ser 695 aag aat ggc ctt gag Lys Asn Gly Leu Giu 685 cct att gcg atc atc Pro Ilie Ala Ile Ile 700 gat gat Asp Asp 690 aac gag gac ctt ctc aac ggc atg Asn Giu Asp Leu Leu Asn Gly Met 705 WO 01/00843 aag acc gtg 2275 Lys Thr Val 710 gtg ctg caa 2323 Vai Leu Gin ccg ttc atg 2371 Pro Phe Met ggc aag ggc 2419 Giy Lys Gly 760 atc ggc aag 2467 Ile Gly Lys 775 gtg gtg aac 2515 Val Val Asn 790 gcg gaa gaa 2563 Ala Glu Glu ctg Leu 715 gaa Giu gaa Giu gtc Val gtg Val atc Ile 795 gca Al a ttt Phe ac c Thr gca Ala gtg Val gac Asp 780 aag Lys gac Asp cag ctg Gin Leu gcc tat Ala Tyr gcg cag Ala Gin 755 gac gtg Asp Val 770 aac ggt Asn Gly atg ttg Met Leu gga. ctt Gly Leu PCTIBOO/00923 cca. ttc Pro Phe 725 ttg gaa Leu Giu 740 gca gag Ala Giu cac gat His Asp tac gac Tyr Asp gaa gca Giu Ala 805 ctt gtg Leu Val 820 aag tcc 2578 Lys Ser acc gtg Thr Val 825 <210> 712 <211> 826 <212> PRT <213> Corynebacteriun glutamicum <400> 712 met Ser Thr Ser Val Thr Ser Pro 2 1 5 Giu Phe Leu Asp Ala Leu Ala Asn 1 Met Giy Thr Gin Leu Gin Gly Phe 2 40 Leu Asp Leu Giu Gly Cys Asn Glu 55 Val Leu Arg Gin Ile His Arg Ala 70 His Asn Asn Ala His Ser 10 Val Leu Ile Gly Asp Gly Leu Asp Val Glu Lys Asp Leu Asn Asp Thr Arg Pro Phe Giu Ala Giy Ala Asp WO 01/00843PC(iO/02 PCT/IBOO/00923 Val

ASP

Ala Arg Gly 145 Leu Gin Met Glu Thr 225 Thr Al a Gly Ala Cys 305 Val1 Gly Ala Met Ala 385 Glu Ile Arg Phe 130 His Gly Asp Ala Thr 210 Ala Gly Asp Lys Leu 290 Cys Gly Pro Ser Ile 370 Met Thr Arg Ala Gly Tyr Asp 165 Gin Asp Thr Pro Glu 245 Val Ala Phe Thr Giu 325 Gin Thr Arg G ly Phe Cys

ASP

Ser Ala 150 Gly Val Thr Met Leu 230 Met Ser Glu Val1 Pro 310 Gin Ala Ser Thr Asp 390 Cys Giu Met 120 Gly Leu Gly Ala Leu 200 Met Ile Glu Met Pro 280 Giu His Thr Arg Pro 360 Ser Glu Asn Leu 105 Gly Pro Arg Asp Ala 185 Pro Gly Asp His Pro 265 Leu Tyr Ile Ser Giu 345 Leu Asn Lys Pro Tyr Gly Thr His 155 Phe His Ile Glu Ile 235 Arg Ala Ala Leu Al a 315 Leu Glu Gin Ser Val1 395 Leu Gly Asn 125 Leu Lys Ile Val1 His 205 Gly Leu Leu Leu Asp 285 Met Arg Lys Glu Thr 365 Al a Ile Ala Thr 110 Giy Pro dlu Giu Gin 190 Val Ala Asn Ser Pro 270 Leu Val1 Asp Ile Asp 350 Gly Phe Ala Asp Ala Met Ser Ala Thr 175 Asp Thr Ala Cys Lys 255 Val Al a Gly Al a Pro 335 Ser Ile Arg Lys Tyr Val Arg Leu Ala 160 Ala Ala Val Leu Ala 240 His Leu Gin Gly Val1 320 Ala Val Ser Glu Gin 400 WO 01/00843 Gin Thr Arg Gly Arg Asp Thr Ser Ser 435 Ilie Arg Thr 450 Val Asn Phe 465 Met Lys Leu Asp Giu Giu Lys Arg Leu 515 Asp Ilie Val 530 Giu Thr Arg 545 Lys Lys Leu Ser Phe Gly Leu Asn Glu 595 Ser Lys Ile 610 Ala Leu Asp 625 Gin Glu Phe Asp Ala Arg Ala Gin Arg 675 Giu Ala Giy 690 Leu Leu Asn 705 Giy 405 Thr Leu Leu Asp Lys 485 Gin Asp Asp Asp Pro 565 Asn Ile Pro Val Gin 645 Giu Ile Lys Met Aia Ala Pro Giu Gly 470 Gin Aia Asp Cys Giy 550 Giu Pro Giu Met Tyr 630 Leu Gin Asp Glu Lys 710 His Asp Ilie His 455 Asp His Arg Ile Leu 535 Ile Ile Ala Ala Asn 615 Asp Phe Leu Gly Lys 695 Thr Leu Leu Ser Arg Ser 475 Vai His Tyr Ile Giu 555 Leu Val Ser Asp Giu 635 Ser Pro Gly Ala Leu 715 Glu Val Ala Giu 445 Ile Tyr Aia Val Leu 525 Thr Ile Leu Asn Ile 605 Gin Tyr Aia Phe Giu 685 Ile Gly Met Asp Leu 430 Pro Vali Gin Leu Arg 510 Asp Gly Arg Ser Ser 590 Ala Arg Asp Asp Giu 670 Asp Asn Ser Lys PCTABOOIOO923 Tyr Val 415 Leu Aia Giu Val Asn Ser Arg Ile 480 Thr Ile 495 Ile Ala Ile Lys Gin Giu Giu Leu 560 Asn Ile 575 Val Phe His Ser Giu Vai Pro Leu 640 Ala Lys 655 Arg Leu Asp Leu Giu Asp Gly Gin 720 Thr Ala Met Gin Leu Pro Phe Val Leu Gin Ser Ala 1000 WO 01/00843. WO 0100843PCTIIBOOIOO923 Val1 Ser Gly Asn 785 Ala Ser Ala Ala Asp 770 Asn Met Gly 725 Glu Glu Asp Asp Ala 805 Val Phe Lys Gly 775 Val Glu Ser Met Gly 760 Lys Asn Glu Thr Glu 745 Lys Asn Leu His Val1 825 730 Glu Glu Ala Glu Ala 750 Ile Val Val Ala Thr 765 Leu Val Asp Ile Ile 780 Gly Ile Lys Gin Pro 795 Lys Ala Asp Val Ilie 810 Val 735 Thr Val Leu Leu Gly 815 Giy Lys Ser Ser 800 Met <210> 713 <211> 2358 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(2335) <223> RXN02085 <400> 713 cacccggtga tttcgcgaac cttgaaacat cgtcagaaga gatccgcacg ttcggctcaa gcagaaagtc tttaactcac tc t Ser aag Lys ctt Leu tct Ser atg Met gac Asp ggt ctt cct cgc Gly Leu Pro Arg ggc Gly cgc Arg gt t Val gc t Ala 75 gaa Glu t ac Tyr caa Gin ccg Pro att Ile aac Asn ttgccgtgcg tcctagccgg atg act tcc aac ttt Met Thr Ser Asn Phe 1 gcg aag cgt gaa ctg Ala Lys Arg Glu Leu att gaa ggt cgc gaa Ile Glu Gly Arg Glu gca tcg gat tct ttg Ala Ser Asp Ser Leu tcc tac tac gac gca Ser Tyr Tyr Asp Ala ccg gag cgt ttt gat Pro Glu Arg Phe Asp ctg tgg att gac cgc Leu Trp Ile Asp Arg 100 WO 01/00843 WO 0100843PCTJIBOO/00923 tac ttt ggc gct Tyr Phe Gly Ala 105 gct cgc ggt Ala Arg Gly act gag acc ctg cct gca Thr Glu Thr Leu Pro Ala 110 acc aag Thr Lys gcg gat Ala Asp 135 cgt tgc Arg Cys 150 cca ctg Pro Leu ttg gat Leu Asp tct ttc Ser Phe gat gtt Asp Val 215 ttg gct Leu Ala 230 gat cag Asp Gln gtt gac Val Asp gag gag Glu Glu acc gac 1027 Thr Asp 295 ggc cca 1075 Gly Pro 310 acc ctc 1123 tgg Trp, 120 ac a Thr cag Gin act Thr cac His gat Asp 200 gc t Al a aag Lys gcg Ala ttg Leu ctg Leu 280 ctg Leu atc Ile gag t tt Phe cgt Arg cag Gln t tC Phe ctg Leu 185 act Thr cc t Pro cgc Arg ctg Leu gtc Val 265 ctg Leu tgt Cys gca Ala gc t gat Asp t tc Phe gtt Val ctt Leu 170 cct Pro gag Glu gag Glu gat Asp aac Asn 250 acc Thr gtt Val gct Ala gtg Val gag acc Thr gtt Val cgt Arg 155 tcc Ser gca Ala tgg Trp gtt Val ggc Gly 235 act Thr cat His gcg Ala gct Ala tct Ser 315 aac aac Asn ttg Leu 140 ggc Gly ctt Leu ctg Leu gtt Val ttg Leu 220 gtg Val ctt Leu 9gc Gly 9gc Gly ct t Leu 300 acc Thr at t tac Tyr 125 gat Asp gt t Val1 gc t Ala tt t Phe cag Gln 205 gag Gl u ttt Phe gcg Ala gtc Val1 atc Ile 285 gct Al a tct Ser gag gtg Val1 ctg Leu 145 cct Pro ga t Asp gag Glu cc t Pro gc t Ala 225 tac Tyr ctt Leu gc t Ala cgt Arg cgc Arg 305 ctg Leu cgc ccg Pro 130 att Ile gtt Val ggt Gly cgc Arg gcg Ala 210 ggt Gly ttc Phe ggc Gly gcg Ala aac Asn 290 ctg Leu c ac His gac cag gca Gin Ala 115 gag ttg Glu Leu gag gat Giu Asp ctg gtt Leu Val tcc aat Ser Asn 180 ctc atc Leu Ile 195 ttg gtc Leu Val tac acc Tyr Thr ggc tct Gly Ser gcg att Ala Ile 260 tgg aag Trp Lys 275 att. tgg Ile Trp gca gct Ala Ala gtt cct Val Pro tgg ctt 1002 WO 01/00843 WO 0100843PCTIiBOO/00923 Thr Leu ttc ggc 1172.

Phe Gly gcc ggc 1219 Ala Gly gct tct 1267 Ala Ser 375 cct ggc 1315 Pro Gly 390 aag tca 1363 Lys Ser cca cag 1411 Pro Gin tcc atc 1459 Ser Ile ctg gtc 1507 Leu Val 455 ggt gag 1555 Giy Giu 470 gac ggt 1603 Asp Giy cgc tgt 1651 Arg Cys cca atg 1699 Pro Met Giu Aia Glu 330 tcg gag aag Ser Giu Lys 345 aac atc gac Asn Ile Asp Asn Ile Giu Pro atc acc gag gtc Ile Thr Glu Val 350 gcg gct gcg ttc Ala Aia Aia Phe 365 tcc cca cgc acc Ser Pro Arg Thr Giu 335 aag Lys Vai Arg Asp Trp, Leu Ala 340 ctg ctt gcc gac gcc cta Leu Leu Aia Asp Ala Leu 355 360 cga Arg gat gcg gcg tcc gca gca att Asp Aia Ala Ser Ala Ala Ile 370 gca cca atc acg cag gaa ctc Ala Pro Ile Thr Gin Glu Leu cgc acc Arg Thr 380 385 cgt agc cgt gga Arg Ser Arg Gly 395 ctg gag ctt cca Leu Giu Leu Pro 410 acc cca tcc att Thr Pro Ser Ile tcc ttc gac act cgt Ser Phe Asp Thr Arg 400 gct ctg cca acc acc Ala Leu Pro Thr Thr 415 cgt tct gct cgc gct Arg Ser Ala Arg Ala gtt acg ctg cag gag Val Thr Leu Gin Giu 405 acc att ggt tct ttc Thr Ile Gly Ser Phe 420 cgt ctg cgc aag gaa Arg Leu Arg Lys Giu 435 425 act ttg Thr Leu gag cag tac gaa Giu Gin Tyr Giu 445 430 gag gca atg cgc gaa gaa atc gat Glu Ala Met Arg Giu Giu Ile Asp 450 gcc aag cag gaa Ala Lys Gin Glu 460 gag cgc aac gac Giu Arg Asn Asp cca Pro gaa ctt ggt ctt Giu Leu Gly Leu atg gtt cag tac Met Val Gin Tyr 480 aac ggc tgg gtc Asn Giy Trp Val gat Asp 465 gtg ttg gtt cac Val Leu Val His 475 ttc ctc Phe Leu gtt cgt Val Arg 505 act gtc Thr Val tca Ser 490 acc Thr gcc Ala ttc tct gaa ctt ctc Phe Ser Giu Leu Leu 485 caa agc tac ggc tcc Gin Ser Tyr Giy Ser 500 gtt tcc cgc cca gcg Val Ser Arg Pro Ala 495 cct cca gtg ttg ttc Pro Pro Val Leu Phe 510 aag tgg ttc cag tac Lys Trp Phe Gin Tyr gga aac Gly Asn 515 gca cag agc ctg acc cag aag Ala Gin Ser Leu Thr Gin Lys 1003 WO 01/00843 WO 0100843PCT/EBOO/00923 cat gtc 1747 His Val 535 ttc gtt 1795 Phe Val 550 ctg gca 1843 Leu Ala atc atc 1891 Ile Ile gac gtc 1939 Asp Val ctg gcg 1987 Leu Ala 615 tgc tac 2035 Cys Tyr 630 gcc gat 2083 Ala Asp gct gct 2131 Ala Ala tgg gat 2179 Ti-p Asp ctc ctc 2227 Leu Leu 695 aac cca 2275 Asn Pro 710 gga atg ctc acc Gly Met Leu Thr 540 525 ggt Gly cca gtc acc Pro Val Thr cgc gat gat cag Arg Asp Asp Gin 555 ctg cgc gat gaa Leu Arg Asp Giu 570 cag gtg gat gag Gin Vai Asp Giu ccg ctg gct acc act Pro Leu Ala Thr Thr 560 att aac gat ctc atc Ile Asn Asp Leu Ile 575 530 atc ctt gca tgg tcc Ile Leu Ala Ti-p Ser 545 gct gac cag gtt gca Ala Asp Gin Val Aia 565 gag gct ggc gcg aag Giu Ala Giy Ala Lys 580 cct gcg Pro Ala .585 gat aag Asp Lys cct gcc tac ctg Pro Ala Tyr Leu 605 att cgt gaa ctg ttg ccg cta cga Ile Arg Giu Leu Leu Pro Leu Arg 590 595 cag tgg tcc gtg gac tcc ttc cgc Gin Trp Ser Val Asp Ser Phe Arg 610 gac gtc caa atc cac acc cac atg Asp Val Gin Ile His Thr His Met gcc ggc gca ccc Ala Gly Ala Pro 620 gac Asp tcc gag ttc aac Ser Giu Phe Asn 635 gtc acc acc atc Val Thr Thr Ile 650 ctg aaa tct tcc Leu Lys Ser Ser gaa Giu gaa 625 gtg atc tcc tcg gtc Val Ile Ser Ser Val 640 atc gcg ttg gat Ile Ala Leu Asp 645 gca gca cgt Ala Ala Ara 655 ggc ttc gag ctc Gly Phe Giu Leu 670 tcc gac atg cag gtc ctc Ser Asp Met Gin Val Leu 660 ggc gtc gga cct ggt gtg Gly Val Gly Pro Giy Val 675 665 atc cac Ilie His tcc ccg cgc gtt Ser Pro Arg Val 685 cct tcc gcg cag aaa gtg gac ggt Pro Ser Ala Gin Lys Val Asp Giy 690 gct gca ctg Ala Ala Leu cag tcc gtg gat cct cgc cag ctg tgg gtc Gin Ser Val Asp Pro Arg Gin Leu Ti-p Val 700 705 aag acc cgt gga tgg cca gaa gtg gaa gct Lys Thr Arg Giy Trp Pro Giu Vai Giu Ala 720 725 gac tgt ggt ctg Asp Cys Gly Leu 715 1004 WO 01/00843 tcc cta aag 2323 Ser Leu Lys gga gca act 2358 Gly Ala Thr PCTIBOOIOO923 gtt ctc gtt gag tcc gct aag cag gct cgt gag aaa atc Val Leu Val Giu Ser Ala Lys Gin Ala Arg Glu Lys Ile 730 735 740 atc taaattgggt taccgctagg aac Ile 745 <210> 714 <211> 745 <212> PRT <213> Corynebacteriun glutanicun <400> 714 Met Thr Ser Asn Phe Ser Ser Thr Val 1 Ala Ile Ala Ser Pro Leu Pro Val Leu 145 Pro Asp Glu Pro Lys Glu Ser Tyr Glu Trp Ala Pro 130 Ile Val Gly Arg Ala 210 Arg Gly Asp Tyr Arg Ile Gin 115 Giu Giu Leu Ser Leu 195 Leu Giu Arg Ser Asp Phe Asp 100 Ala Leu Asp Val Asn 180 Ile Val 5 Leu Glu Leu Ala Asp Arg Met Ser Leu Gly 165 Pro Lys Thr Phe Al a Gly 55 Leu Ile Phe Lys Asp 135 Cys Leu Asp Phe Val 215 Gly Gly Arg Val Ala 75 Giu Arg Thr Val Arg 155 Ser Ala Trp Val1 Leu Tyr Gin Pro Ile Asn Gly Asn Leu 140 Gly Leu Leu Val Leu 220 Pro Trp Leu Phe Leu Asp Thr Tyr 125 Asp Val1 Ala Phe Gin 205 Glu Arg Asn Val Ala Gly Gly Glu 110 His Al a Asn Arg Glu 190 Ile Gin Val1 Ile Gly Asn Gly Val Leu Thr Tyr Ser Ala Thr 175 Val Asp Val1 Asn Gly Ser Thr Arg Leu Pro Leu Leu Ala Arg 160 Thr Tyr Glu Arg Thr Ala Gly Tyr Thr Thr Leu Ala Lys Arg Asp Gly Val Phe 1005 WO 01/00843 225 Tyr Phe GI Leu Gly Al Ala Ala Tr Arg Asn Il 290 Arg Leu Al 305 Leu His Va Arg Asp Tr Leu Ala As Ala Ser Al 370 Ile Thr Gi 385 Val Thr Le Thr Ile Gi Arg Leu Ax 43 Arg Giu Gi 450 Asp Val Le 465 Phe Ser Gi Gin Ser Ty Val Ser Ar 51 Ser Leu Th 530 Ile Leu Al 545 230 Asp Val Giu Thr Gly 310 Thr Phe Al a Ala Pro 390 Lys Pro Ser Leu Gly 470 Asp Arg Pro His Phe 550 Gin Asp Giu Asp 295 Pro Leu Gly Gly Ser 375 Gly Ser Gin Ile Val1 455 Giu Gly Cys Met Val 535 Val1 Ala Leu Leu 280 Leu Ile Glu Ser Asn 360 Arg Arg Leu Thr Thr 440 Ile Pro Phe Val1 Thr 520 Lys Arg Leu Vai 265 Leu Cys Aia Ala Giu 345 Ile Arg Ser Giu Pro 425 Leu Ala Giu Leu Arg 505 Val1 Giy Asp 235 Thr His Ala Al a Ser 315 Asn Ile Aia Ser Gly 395 Pro Ile Gin Gin Asn 475 Thr Pro Trp Leu Gin 555 Giy Thr 270 Val S er Cys Pro Val 350 Phe Thr Asp Pro Ala 430 Giu Leu Vali Gly Phe 510 Tyr Pro Ala PCTIIBOO/00923 240 Ile Giy 255 Giu Leu Asp Giy Leu Lys Ser Leu 320 Giu Val 335 Lys Leu Asp Ala Ala Pro Thr Arg 400 Thr Thr 415 Arg Ala Ala met Gly Leu Gin Tyr 480 Trp Vai 495 Gly Asn Ala Gin Val Thr Thr Thr 560 1006 WO 01/00843 WO 0100843PCTIIBOOIOO923 Ala Asp Gin Val Ala Leu Ala Leu Arg Asp Giu Ile Asn Asp Leu Ile 565 570 575 Giu Ala Gly Ala Lys Ile Ile Gin Val Asp Giu Pro Ala Ile Arg Giu 580 585 590 Leu Leu Pro Leu Arg Asp Val Asp Lys Pro Ala Tyr Leu Gin Trp Ser 595 600 605 Val Asp Ser Phe Arg Leu Ala Thr Ala Gly Ala Pro Asp Asp Val Gin 610 615 620 Ile His Thr His Met Cys Tyr Ser Giu Phe Asn Giu Val Ile Ser Ser 625 630 635 640 Val Ilie Ala Leu Asp Ala Asp Val Thr Thr Ile Giu Ala Ala Arg Ser 645 650 655 Asp Met Gin Val Leu Ala Ala Leu Lys Ser Ser Gly Phe Giu Leu Gly 660 665 670 Val Gly Pro Gly Val Trp Asp Ile His Ser Pro Arg Val Pro Ser Ala 675 680 685 Gin Lys Val Asp Gly Leu Leu Giu Ala Ala Leu Gin Ser Val Asp Pro 690 695 700 Arg Gin Leu Trp Val Asn Pro Asp Cys Gly Leu Lys Thr Arg Gly Trp 705 710 715 720 Pro Giu Val Giu Ala Ser Leu Lys Val Leu Val Giu Ser Ala Lys Gin 725 730 735 Ala Arg Giu Lys Ile Gly Ala Thr Ile 740 745 <210> 715 <211> 1923 <212> DNA <213> Corynebacterium giutamicun <22 0> <221> CDS <222> (101)..(1900) <223> FRXA02085 <400> 715 cacccggtga tttcgcgaac cttgaaacat cgtcagaaga ttgccgtgcg tcctagccgg gatccgcacg ttcggctcaa gcagaaagtc tttaactcac atg act tcc aac ttt 115 Met Thr Ser Asn Phe 1 tct tcc act gtc gct ggt ctt cct cgc atc gga gcg aag cgt gaa ctg 163 Ser Ser Thr Val Ala Gly Leu Pro Arg Ile Gly Ala Lys Arg Giu Leu 15 aag ttc gcg ctc gaa ggc tac tgg aat gga tca att gaa ggt cgc gaa 211 Lys Phe Ala Leu Giu Gly Tyr Trp, Asn Gly Ser Ile Giu Gly Arg Giu 1007 WO 01/00843 WO 0100843PCTIIIBOO/00923 30 ctt gcg cag acc gcc cgc caa ttg gtc aac act gca tcg gat tct ttg 259 Leu Ala Gin Thr Ala Arg Gln Leu Val Asn Thr Ala Ser Asp Ser Leu 45 tct gga ttg gat tcc gtt ccg ttt gca gga cgt tcc tac tac gac gca 307 Ser Gly Leu Asp Ser Val Pro Phe Ala Gly Arg Ser Tyr Tyr Asp Ala 60* atg ctc gat acc gcc gct att ttg ggt gtg ctg ccg gag cgt ttt gat 355 Met Leu Asp Thr Ala Ala Ile Leu Gly Val Leu Pro Glu Arg Phe Asp 75 80 gac atc gct gat cat gaa aac gat ggt ctc cca ctg tgg att gac cgc 403 Asp Ile Ala Asp His Glu Asn Asp Gly Leu Pro Leu Trp Ile Asp Arg 95 100 tac ttt ggc gct gct cgc ggt act gag acc ctg cct gca cag gca atg 451 Tyr Phe Gly Ala Ala Arg Gly Thr Giu Thr Leu Pro Ala Gin Ala Met 105 110 115 acc aag tgg ttt gat acc aac tac cac tac ctc gtg ccg gag ttg tct 499 Thr Lys Trp Phe Asp Thr Asn Tyr His Tyr Leu Val Pro Giu Leu Ser 120 125 130 gcg gat aca cgt ttc gtt ttg gat gcg tcc gcg ctg att gag gat ctc 547 Ala Asp Thr Arg Phe Val Leu Asp Ala Ser Ala Leu Ilie Glu Asp Leu 135 140 145 cgt tgc cag cag gtt cgt ggc gtt aat gcc cgc cct gtt ctg gtt ggt 595 Arg Cys Gin Gin Val Arg Gly Val Asn Ala Arg Pro Vai Leu Val Giy 150 155 160 165 cca ctg act ttc ctt tcc ctt gct cgc acc act gat ggt tcc aat cct 643 Pro Leu Thr Phe Leu Ser Leu Ala Arg Thr Thr Asp Gly Ser Asn Pro 170 175 180 ttg gat cac ctg cct gca ctg ttt gag gtc tac gag cgc ctc atc aag 691 Leu Asp His Leu Pro Ala Leu Phe Giu Val Tyr Glu Arg Leu Ile Lys 185 190 195 tct ttc gat act gag tgg gtt cag atc gat gag cct gcg ttg gtc acc 739 Ser Phe Asp Thr Giu Trp Vai Gin Ile Asp Giu Pro Ala Leu Val Thr 200 205 210 gat gtt gct cct gag gtt ttg gag cag gtc cgc gct ggt tac acc act 787 Asp Val Ala Pro Giu Val Leu Giu Gin Val Arg Ala Gly Tyr Thr Thr 215 220 225 ttg gct aag cgc gat ggc gtg ttt gtc aat act tac ttc ggc tct ggc 835 Leu Ala Lys Arg Asp Giy Val Phe Vai Asn Thr Tyr Phe Gly Ser Gly 230 235 240 245 gat cag gcg ctg aac act ctt gcg ggc atc ggc ctt ggc gcg att ggc 883 Asp Gin Ala Leu Asn Thr Leu Ala Gly Ile Gly Leu Gly Ala Ile Gly 250 255 260 gtt gac ttg gtc acc cat ggc gtc act gag ctt gct gcg tgg aag ggt 931 Val Asp Leu Val Thr His Gly Val Thr Glu Leu Ala Ala Trp Lys Gly 265 270 275 1008 WO 01/00843 gag gag ctg Glu Giu Leu 280 PCT/IBOO/00923 ctg gtt gcg ggc Leu Val Ala Gly atc Ile 285 gct Ala acc gac 1027 Thr Asp 295 ggc cca 1075 Gly Pro 310 acc ctc 1123 Thr Leu ttc ggc 1171 Phe Gly gcc ggc 1219 Ala Gly gct tct 1267 Ala Ser 375 cct ggc 1315 Pro Gly 390 aag tca 1363 Lys Ser cca cag 1411 Pro Gin tcc atc 1459 Ser Ilie ctg gtc 1507 Leu Val 455 ctg tgt gct gct ctt Leu Cys Ala Ala Leu 300 atc gca gtg tct acc Ile Ala Val Ser Thr 315 gag gct gag aac att Giu Ala Giu Asn Ile gtt gat ggt Vai Asp Giy tcc ctg aag Ser Leu Lys tct tgt tca ctg Ser Cys Ser Leu 320 gag cct gag gtc Giu Pro Glu Val 335 gag gtc aag ctg Glu Val Lys Leu cgt aac att tgg cgc 979 Arg Asn Ile Trp Arg 290 cgc ctg gca gct cgc Arg Leu Ala Ala Arg 305 ctg cac gtt cct tac Leu His Val Pro Tyr 325 cgc gac tgg ctt gcc Arg Asp Trp Leu Ala 340 ctt gcc gac gcc cta Leu Ala Asp Ala Leu 330 tcg gag Ser Glu 345 aac atc Asn Ilie aag atc acc Lys Ile Thr 350 gac gcg gct gcg Asp Ala Ala Ala 365 t tc Phe acc Thr 355 gat gcg gcg tcc gca gca att Asp Ala Ala Ser Ala Ala Ile 370 gca cca atc acg cag gaa ctc Ala Pro Ile Thr Gin Glu Leu cga cgc acc tcc cca Arg Arg Thr Ser Pro 380 cgt agc cgt gga tcc Arg Ser Arg Giy Ser cgc Arg ttc gac act cgt Phe Asp Thr Arg ctg gag ctt Leu Giu Leu 410 acc cca tcc Thr Pro Ser cca gct ctg cca acc Pro Ala Leu Pro Thr 415 att cgt tct gct cgc Ile Arg Ser Ala Arg 430 385 gtt acg ctg cag gag Val Thr Leu Gin Glu 405 acc att ggt tct ttc Thr Ile Gly Ser Phe 420 cgt ctg cgc aag gaa Arg Leu Arg Lys Glu gc t Aila act Thr 425 435 ttg gag cag tac gaa Leu Giu Gin Tyr Glu 445 gcc aag cag gaa gaa Ala Lys Gin Glu Glu 460 gag gca atg cgc gaa gaa atc gat Giu Ala Met Arg Glu Glu Ile Asp 450 ctt ggt ctt gat gtg ttg gtt cac Leu Gly Leu Asp Val Leu Val His 465 1009 WO 01/00843 ggt gag cca 1555 Gly Glu Pro 470 gac ggt ttc 1603 Asp Giy Phe cgc tgt gtt 1651 Arg Cys Val cca atg act 1699 Pro Met Thr 520 cat gtc aag 1747 His Val Lys 535 ttC gtt cgc 1795 Phe Val Arg 550 ctg gca ctg 1843 Leu Ala Leu atc atc cag 1891 Ile Ile Gin PCTJIBOO/00923 gag cgc aac Giu Arg Asn 475 ctc tca acc Leu Ser Thr 490 cgt cct cca Arg Pro Pro 505 gtc aag tgg Vai Lys Trp gac atg gtt cag tac Asp Met Val Gin Tyr 480 gcc aac ggc tgg gtc Ala Asn Gly Trp Vai 495 gtg ttg ttc gga aac Vai Leu Phe Giy Asn ttc tct gaa ctt ctc Phe Ser Giu Leu Leu 485 caa agc tac ggc tcc Gin Ser Tyr Gly Ser 500 gtt tcc cgc cca gcg Val Ser Arg Pro Ala 515 510 ttc cag Phe Gin tac Tyr gca cag agc ctg acc cag aag Ala Gin Ser Leu Thr Gin Lys 530 gga atg ctc acc Giy Met Leu Thr 525 gat gat cag Asp Asp Gin 555 cgc gat gaa Arg Asp Glu ggt cca gtc acc atc Gly Pro Val Thr Ile 545 ctg gct acc act gct Leu Ala Thr Thr Ala 560 aac gat ctc atc gag Asn Asp Leu Ile Giu ctt gca tgg tcc Leu Ala Trp, Ser gac cag gtt gca Asp Gin Val Ala 565 gct ggc gcg aag Ala Giy Aia Lys att Ile 570 gtg gat 'Iai Asp 585 575 gag cct gcg att cgt Giu Pro Ala Ile Arg 590 580 gaa ctg ttg ccc gct acg Glu Leu Leu Pro Ala Thr 595 aga cgt 1923 Arg Arg cga Arg 600 taagcctgcc tacctgcagt ggt <210> 716 <211> 600 <212> PRT <213> Corynebacteriun giutamicum* <400> 716 Met Thr Ser Asn Phe Ser Ser.Thr Val 1 5 Ala Lys Arg Giu Leu Lys Phe Ala Leu 25 Ile Giu Gly Arg Giu Leu Ala Gin Thr 40 Ala Gly Leu Pro Arg Ile Gly 10 Giu Gly Tyr Trp Asn Gly Ser Ala Arg Gin Leu Val Asn Thr 1010 WO 01/00843 Ala Ser As Ser Tyr TY Pro Glu Ar Leu Trp Il Pro Ala Gi 11 Val Pro Gi 130 Leu Ilie Gi 145 Pro Val Le Asp Gly Se Glu Arg Le 19 Pro Ala Le 210 Ala Gly Ty 225 Tyr Phe Gi Leu Gly Al.

Ala Ala Tr 27 Arg Asn Il 290 Arg Leu Al 305 Leu His Va Arg Asp Tr Leu Ala As Ala Ser Al p e n 5

U

U

U

r u 5

U

r y a p 5 e a 1 p p 5 a Ser Asp Phe Asp 100 Ala Leu Asp Val1 Asn 180 Ile Val Thr Ser Ile 260 Lys Trp Al a Pro Leu 3 40 Al a Al a Leu Ala Asp Arg Met Ser Leu Gly 165 Pro Lys Thr Thr Gly 245 Gly Gly Arg Arg Tyr 325 Ala Leu le Ser Met 70 Asp Tyr Thr Ala Arg 150 Pro Leu Ser Asp Leu 230 Asp Val Glu Thr Gly 310 Thr Phe Ala Ala Gly 55 Leu I le Phe Lys Asp 135 Cys Leu

ASP

Phe Val 215 Ala Gin Asp Glu Asp 295 Pro Leu Gly Gly Ser Leu Asp Ala Gly Trp 120 Thr Gin Thr His Asp 200 Ala Lys Ala Leu Leu 280 Leu Ile Glu Ser Asn 360 Arg

ASP

Thr Asp Ala 105 Phe Arg Gln Phe Leu 185 Thr Pro Arg Leu Val 265 Leu Cys Ala Ala Glu 345 Ile Arg Ser Ala His 90 Ala Asp Phe Val Leu 170 Pro Glu Glu Asp Asn 250 Thr Val Ala Val1 Gl u 330 Lys Asp Val Ala 75 Glu Arg Thr Val Arg 155 Ser Ala Trp Val Gly 235 Thr His Ala Ala Ser 315 Asn Ile Ala Pro Ile Asn Gly Asn Leu 140 G ly Leu Leu Val Leu 220 Val Leu G ly Gly Leu 300 Thr Ile Thr Al a Phe Leu Asp Thr Tyr 125 Asp Val1 Ala Phe Gin 205 Glu Phe Al a Val1 I le 285 Al a Ser Glu Glu Al a 365 Ala Gly Gly Glu 110 His Al a Asn Arg Glu 190 I le Gin Val Gly Thr 270 Val Ser Cys Pro Val 350 Phe PCT/1B00100923 Gly Arg Val Leu Leu Pro Thr Leu Tyr Leu Ser Ala Ala Arg 160 Thr Thr 175 Val Tyr Asp Glu Val Arg Asn Thr 240 Ile Gly 255 Glu Leu Asp Gly Leu Lys Ser Leu 320 Giu Val 335 Lys Leu Asp Ala Thr Ser Pro Arg Thr Ala Pro 1011 WO 01/00843 370 Ile Thr Gi 385 Val Thr Le Thr Ile Gi Arg Leu Ax 43 Arg Giu Gi 450 Asp Val Le 465 Phe Ser Gi Gin Ser T-j Val Ser Ar 51 Ser Leu Tli 530 Ilie Leu Al 545 Ala Asp Gi Giu Ala Gi Leu Leu Pr PCT/EBO0100923 Arg Leu Thr Thr 440 Ile Pro Phe Val Thr 520 Lys Arg Leu Gin Arg 600 Ser Giu Pro 425 Leu Ala Glu Leu Arg 505 Val Gly Asp Arg Val 585 Arg Leu 410 Ser Giu Lys Arg Ser 490 Pro Lys Met Asp Asp 570 Asp Gly 395 Pro Ile Gin Gin Asn 475 Thr Pro Trp Leu Gin 555 Giu Glu 380 Ser Ala Arg Tyr Giu 460 Asp Ala Val Phe Thr 540 Pro Ile Pro <210> 717 <211> 603 <212> DNA <213> Corynebacteriui giutamicui <220> <221> CDS <222> (101)..(580) <223> FRXA02086 <400> 717 gatgatcagc cgctggctac cactgctgac caggttgcac tggcactgcg cgatgaaatt aacgatctca tcgaggctgg cgcgaagatc atccaggtgg atg agc ctg cga ttc 115 Met Ser Leu Arg Phe 1 1012 WO 01/00843 WO 0100843PCTIBOOIOO923 gtg Val1 tgg Trp gtc Val tcc Ser cgt Arg ctc Leu tcc Ser gat Asp gga Gly aac Asn tcc Ser caa Gin tcg Ser tc c Ser ggc Gly gcg Al a cc t Pro tgg Trp 135 tgt Cys gtg Val atc Ile gtc Val1 gac

ASP

gtc Val cag Gin cgc Arg 120 cca Pro tgc Cys gac Asp cac His atc Ile atg Met gga Gly aa a Lys 105 cag Gin gaa Glu gat Asp act Thr tcc Ser gtc Val ctg Leu atc Ile gag Glu gac Asp aag Lys aag cct gcc tac Lys Pro Ala Tyr gcc ggc gca ccc Ala Gly Ala Pro gag ttcaac gaa Glu Phe Asn Glu acc acc atc gaa Thr Thr Ile Glu aaa. tct tcc ggc Lys Ser Ser Gly 80 cac tcc ccg cgc His Ser Pro Arg gct gca ctg cag Ala. Ala Leu Gin 115 tgt ggt ctg aag Cys Gly Leu Lys 130 gtt ctc gtt gag Val Leu Val Glu 145 ctg cag Leu Gin gac gac Asp Asp gtg atc Val Ile gca gca Ala Ala ttc gag Phe Glu gtt cct Val Pro 100 tcc gtg Ser Val acc cgt Thr Arg tcc gct Ser Ala 163 211 259 307 355 403 451 499 547 aag cag gct cgt gag aaa atc gga gca act atc taaattgggt taccgctagg 600 Lys Gin Ala Arg Glu Lys Ile Gly Ala Thr Ile 150 155 160 aac 603 <210> 718 <211> 160 <212> PRT <213> Corynebacterium giutanticum <400> 718 Met Ser Leu Arg Phe Val Asn Cys Cys Pro Leu Arg Asp Val Asp Lys 1 5 10 Pro Ala Tyr Leu Gln Trp Ser Val Asp Ser Phe Arg Leu Ala Thr Ala 25 Gly Ala Pro Asp Asp Val Gin Ile His Thr His Met Cys Tyr Ser Glu 40 Phe Asn Glu Val Ile Ser Ser Val Ile Ala Leu Asp Ala Asp Val Thr 55 1013 WO 01/00843 WO 0100843PCT/IBOO/00923 Thr Ser Ser Al a Gly Leu 145 Giu Gly Arg Gin 115 Lys Giu Asp Val1 Gin Arg 120 Pro Ala Met Gly Lys 105 Gin Giu Arg Gin Pro Val Leu Val Glu Val1 75 Gly Asp Trp Glu Lys 155 Leu Ile Giu Asp Lys Thr Lys His Al a Cys Val1 Ile 160 <210> 719 <211> 1326 <212> DNA <213> Corynebacterium glutamicun <220>- <221> CDS <222> (101)..(1303) <223> RXN02648 <400> 719 atgaataaaa ttccgggtgc agtgaccgta ggtgaggtaa gagtttgata ctttctttcg acttttagat tggattttca atc Ile gat Asp ttc Phe gac Asp tcc Ser ctg ggt Gly tct Ser tct Ser ctt Leu ggt Gly acc tcc Ser aac Asn gta Val aac Asn gca Ala gat ccc Pro gag Giu gac Asp ggc Gly tgg Trp gac acgcggttag agtcgaatga atg agc cag aac cgc Met Ser Gin Asn Arg 1 acc cca gag cta ctt Thr Pro Giu Leu Leu ggg gag gag gaa ttc Gly Giu Giu Glu Phe atc aag cgc cag gtt Ile Lys Arg Gin Val tac ggc cac gtc acc Tyr Gly His Val Thr tac tcc ttc acc cgc Tyr Ser Phe Thr Arg tgg gca agc cag gaa 115 163 211 259 307 355 403 1014 WO 01/00843 Leu Gly Gly gca gtg cgt Ala Val Arg cgt cgc gac Arg Arg Asp 120 ggc atc ttc Gly Ilie Phe 135 cct att acc Pro Ile Thr 150 ctg aag aag Leu Lys Lys gca cta tcc Ala Leu Ser act gat gaa Thr Asp Glu 200 tac aag atc Tyr.Lys Ilie 215 gac ttg gca Asp Leu Ala 230 gat tac ttg Asp Tyr Leu gtg aag ggc Val Lys Gly tct tgg cac Ser Trp His 280 ggt gag atc 1027 Giy Glu Ile 295 tct cct cgt 1075 Ser Pro Arg 310 Leu tcc Ser 105 cgc Arg ac c Thr tac Tyr ggc Gly cca Pro 185 gaa Giu atc Ile gaa Glu gac Asp ctt Leu 265 gga Giy ctg Leu cac His Thr acc Thr gc a Ala ggt Gly att Ile atg Met 170 gga Giy gtC Val1 ac c Thr gca.

Al a tgg Trp 250 cca Pro cca Pro cgc Arg gca Ala Met cc t Pro t tg Leu cgc Arg ggC Gly 155 aac Asn tc t Ser gtc Val1 gat Asp tgg Trp 235 atc Ile aag Lys cac His gca Ala cac His 315 Thr ggC Gly ttc Phe gc t Ala 140 cag Gin gca Ala gca Ala gca Al a gca Ala 220 gat Asp ggt Gly gaa Glu gtc Val gag Glu 300 gag Glu Asp aac Asn agc Ser 125 tct Ser gaa Glu gcg Ala gct Ala gca Ala 205 ggt Gly cag Gin aca Thr cag Gin act Thr 285 gtc Val tgg Trp Thr atc Ile 110 gaa Giu gtg Val gaa Giu gga Gly cga Arg 190 tgt Cys c tg Leu atc Ile cgc Arg acc Thr 270 gac Asp ggt Gly cgt Arg Asp 95 gag Glu gca Ala ggc Gly act Thr gct Ala i7 5 ttg Leu gct Ala acc Thr aac Asn atc Ile 255 cgc Arg atc Ile ggc Gly gta Arg ctg Leu tac Tyr aac Asn cag Gin 160 acc Thr acc Thr gat Asp gtt Val cca Pro 240 gat Asp ctg Leu cca Pro ttc Phe tgg Trp acc Thr gag Giu c ca Pro 145 acg Thr gac Asp aac *Asn gcg Ala cag Gin 225 gag Glu gcc Ala c ac His ttc Phe tcc Ser 305 Al a agc Ser gat Asp 130 gag Glu ga t Asp ggc Gly aag Lys ctt Leu 210 ctc Leu cca Pro atc Ile atc Ile ggt Giy 290 ttc Phe PCTIIBOO/00923 Ser Gln Giu 100 ttc tct gat 451 Phe Ser ASP 115 cca gta tct 499 pro Val Ser ttc acc gga 547 Phe Thr Gly gtt gat ctg 595 Val Asp Leu 165 ttc gtt gca 643 Phe Val Ala 180 ttc tac gac 691 Phe Tyr Asp 195 tcc cag gaa 739 Ser Gin Giu gac gca ccg 787 Asp Ala Pro agc gtg aag 835 Ser Val Lys 245 aac agt gca 883 Asn Ser Ala 260 tgc tgg ggc 931 Cys Trp Gly 275 gac atc att 979 Asp Ile Ile gaa ggc gca Giu Gly Ala gaa gaa aac aag ctt Glu Glu Asn Lys Leu Val Trp 320 1015 WO 01/00843 cct gaa ggc tct gtt atc tac cct ggt 1123 Pro Giu Gly Ser Val Ile Tyr Pro Gly 330 gct gtg gag cac cca cgc ctg gtt gct 1171 Ala Val Giu His Pro Arg Leu Val Ala 345 350 aag ctt gtt ggc cct gag aac gtc att 1219 Lys Leu Val Gly Pro Glu Asn Val Ile 360 365 ggc gga cgt ctg cat tcc cag atc gca 1267 Gly Gly Arg Leu His Ser Gin Ile Ala 375 380 gta gag ggc gct cgc att gca tca aag 1313 Val Glu Gly Ala Arg Ile Ala Ser Lys 390 395 aacgagggtt gct 1326 <210> 720 <211> 401 <212> PRT <213> Corynebacteriui giutanicui <400> 720 Met Ser Gin.Asn Arg Ile Arg Thr Thr 1 Thr Pro Giu Leu Leu Asp Ala Asn Ile 25 Giy Glu Glu Giu Phe Phe Gin Ile Leu 40 Ilie Lys Arg Gin Val Asp Leu Gly Ile 55 Tyr Gly His Val Thr Ser Gly Ala Val 70 Tyr Ser Phe Thr Arg Leu Gly Gly Leu Trp.Ala Ser Gin Giu Ala Val Arg Ser 100 105 Thr Ser Phe Ser Asp Arg Arg Asp Arg 115 120 Glu Asp Pro Val Ser Gly Ile Phe Thr 130 135 gtg Val cgt Arg tcc Ser gca Al a ctg Leu 400 Val1 Axg Ser Ile Phe Met Pro Leu Arg tct Ser atc Ile act Thr aag Lys 385 t tc Phe Gly Ser Ser Leu Gly Thr Gly Phe Ala 140 PCTIBOOIOO923 cac tcc atc aac His Ser Ile Asn 340 gtt cag ttc gcc Val Gin Phe Ala 355 gac tgt ggt ctg Asp Cys Gly Leu 370 ctg gag tcc cta Leu Giu Ser Leu taagctagac Ser Asn Val Asn Al a Asp Asn Ser 125 Ser Leu Gly Asp Giu Trp Thr Ile 110 Giu Val1 1016 WO 01/00843 WO 0100843PCTJIBOOIOO923 Pro Glu Phe Thr Gly Pro Ile Thr Tyr Ile Gly Gin Glu Giu Thr Gin 145 150 155 160 Thr Asp Vai Asp Leu Leu Lys Lys Gly Met Asn Ala Ala Gly Ala Thr 165 170 175 Asp Gly Phe Val Ala Ala Leu Ser Pro Gly Ser Ala Ala Arg Leu Thr 180 185 190 Asn Lys Phe Tyr Asp Thr Asp Giu Giu Val Val Ala Ala Cys Ala Asp 195 200 205 Ala Leu Ser Gin Giu Tyr Lys Ile Ile Thr Asp Ala Gly Leu Thr Val 210 215 220 Gin Leu Asp Ala Pro Asp Leu Ala Giu Ala Trp Asp Gin Ile Asn Pro 225 230 235 240 Giu Pro Ser Val Lys Asp Tyr Leu Asp Trp Ile Gly Thr Arg Ile Asp 245 250 255 Ala Ile Asn Ser Ala Val Lys Gly Leu Pro Lys Giu Gin Thr Arg Leu 260 265 270 His Ile Cys Trp Gly Ser Trp His Gly Pro His Val Thr Asp Ile Pro 275 280 285 Phe Gly Asp Ile Ile Gly Gu Ile Leu Arg Ala Giu Val Gly Gly Phe 290 295 300 Ser Phe Glu Gly Ala Ser Pro Arg His Ala His Giu Trp Arg Val Trp 305 310 315 320 Giu Giu Asn Lys Leu Pro Giu Gly Ser Val Ile Tyr Pro Gly Val Val 325 330 .335 Ser His Ser Ile Asn Ala Val Giu His Pro Arg Leu Val Ala Asp Arg 340 345 350 Ilie Val Gin Phe Ala Lys Leu Val Gly Pro Glu Asn Val Ile Ala Ser 355 360 365 Thr Asp Cys Gly Leu Gly Gly Arg Leu His Ser Gin Ile Ala Trp Ala 370 375 380 Lys Leu Glu Ser Leu Val Giu Gly Ala Arg Ile Ala Ser Lys Giu Leu 385 390 395 400 Phe <210> 721 <211> 548 <212> DNA <213> Corynebacteriun glutamicum <2 <221> CDS <222> 1017 WO 01/00843 PCTIBOOIOO923 <223> FRXA02648 <400> 721 gac gca ccg gac ttg gca gaa gca tgg gat cag atc aac cca gag cca 48 Asp Ala Pro Asp Leu Ala Glu Ala Trp Asp Gin Ile Asn Pro Giu Pro 1 5 10 agc gtg aag gat tac ttg gac tgg atc ggt aca 6gc atc gat .gcc atc 96 Ser Val Lys Asp Tyr Leu Asp Trp Ile Gly Thr Arg Ilie Asp Ala Ile 25 aac agt gca gtg aag ggc ctt cca aag gaa cag acc cgc ctg cac atc 144 Asn Ser Ala Val Lys Gly Leu Pro Lys Giu Gin Thr Arg Leu His Ile 40 tgc tgg ggc tct tgg cac gga cca cac gtc act gac atc cca ttc ggt 192 Cys Trp Giy Ser Trp His Gly Pro His Val Thr Asp Ile Pro Phe Gly 55 gac atc att ggt gag atc ctg cgc gca gag gtc ggt ggc ttc tcc ttc 240 Asp Ilie Ilie Gly Giu Ilie Leu Arg Ala Giu Val Gly Giy Phe Ser Phe 70 75 gaa ggc gca tct cct cgt cac gca cac gag tgg cgt gta tgg gaa gaa 288 Giu Gly Ala Ser Pro Arg His Ala His Giu Trp Arg Val Trp, Giu Giu 90 aac aag ctt cct gaa ggc tct gtt atc tac cct ggt gtt gtg tct cac 336 Asn Lys Leu Pro Giu Gly Ser Val Ile Tyr Pro Gly Val Val Ser His 100 105 110 tcc atc aac gct gtg gag cac cca cgc ctg gtt gct gat cgt atc gtt 384 Ser Ile Asn Ala Val Glu His Pro Arg Leu Val Ala Asp Arg Ile Val 115 120 125 cag ttc gcc aag ctt gtt ggc cct gag aac gtc att gcg tcc act gac 432 Gin Phe Ala Lys Leu Val Gly Pro Giu Asn Val Ile Ala Ser Thr Asp 130 135 140 tgt ggt ctg ggc gga cgt ctg cat tcc cag atc gca tgg gca aag ctg 480 Cys Gly Leu Gly Gly Arg Leu His Ser Gin Ile Ala Trp, Ala-Lys Leu 145 150 155 160 gag tcc cta gta gag ggc gct cgc att gca tca aag gaa ctg ttc 525 Giu Ser Leu Val Giu Gly Ala Arg Ilie Ala Ser Lys Giu Leu Phe 165 170 175 taagctagac aacgagggtt gct 548 <210> 722 <211> 175 <212> PRT <213> Corynebacteriun giutamicum <400> 722 Asp Ala Pro Asp Leu Ala Giu Ala Trp, Asp Gin Ile Asn Pro Glu Pro 1 5 10 Ser Val Lys Asp Tyr Leu Asp Trp Ile Gly Thr Arg Ile Asp Ala Ile 25 1018 WO 01/00843 WO 0100843PCT/EBOO/00923 Asn Cys Asp Giu Asn Ser Gin Cys 145 Giu Val Ser Gly Ser Pro 100 Ala Lys Gly Val Lys Trp Giu Pro Giu Val1 Leu Gly Glu 165 Gly Hius Ile 70 Arg Gly Glu Val1 Arg 150 Gly Thr Asp Gly Arg Giy Ala Ile 140 Ala Lys Arg Ile Gly Val Val Asp 125 Ala Trp Glu Leu Pro Phe Trp Val1 110 Arg Ser Ala Leu His Phe Ser Giu Ser Ile Thr Lys Phe 175 Ile Gly Phe Giu His Val Asp Leu 160 <210> 723 <211> 784 <212> DNA <213> Corynebacteriun giutamicum <22 0> <221> CDS <222> (101)..(784) <223> FRXA02658 <400> 723 atgaataaaa ttccgggtgc agtgaccgta ggtgaggtaa gagtttgata ctttctttcg acttttagat tggattttca atc agg Ile Arg gat gca Asp Ala ttc cag Phe Gin gac ctg Asp Leu acc act Thr Thr aac atc Asn Ile att ctg Ile Leu ggt atc Gly Ile cac gtt His Vai aag cgt Lys Arg cag tct Gin Ser gac atc Asp Ile tcc ttg ccc Ser Leu Pro 15 aac ggt gag Asn Gly GlU 30 gta gat gac Val Asp Asp 45 aac gag ggc Asn Giu Gly acgcggttag agtcgaatga atg agc cag aac cgc Met Ser Gin Asn Arg 1 acc cca gag cta ctt Thr Pro Giu Leu Leu ggg gag gag gaa ttc Gly Glu Glu Giu Phe atc aag cgc cag gtt Ile Lys Arg Gin Val tac ggc cac gtc acc Tyr Giy His Vai Thr 115 163 211 259 307 1019 WO 01/00843 WO 0100843PCT/BOO/00923 tcc ggt gca gtt gac ttc Ser Gly Ala Val Asp Phe gg t Gly gca tgg tgg aac Ala Trp Trp Asn ctg ggc gga ctg acc atg acc gat Leu Gly Gly Leu Thr Met Thr Asp gca gtg cgt tcc acc cct ggc aac Ala Val Arg Ser Thr Pro Gly Asn 105 cgt cgc gac cgc gca ttg ttc agc Arg Arg Asp Arg Ala Leu Phe Ser 120 125 ggc atc ttc acc ggt cgc gct tct Gly Ilie Phe Thr Gly Arg Ala Ser 135 140 cct ,att acc tac att ggc cag gaa Pro Ilie Thr Tyr Ile Gly Gln Glu 150 155 ctg aag aag ggc atg aac gca gcgI Leu Lys Lys Gly Met Asn Ala Ala 170 gca cta tcc cca gga tct gca gct Ala Leu Ser Pro Gly Ser Ala Ala 185 act gat gaa gaa gtc gtc gca gca I Thr Asp Giu Giu Val Val Ala Ala 200 205 tac aag atc atc acc gat gca ggt Tyr Lys Ile Ile Thr Asp Ala Gly 1 215 220 <210> 724 <211> 228 <212> PRT <213> Corynebacteriun glutainicum .<400> 724 Met Ser Gin Asn Arg Ile Arg Thr 1 5 Thr Pro Giu Leu Leu Asp Ala Asn Giy Giu Giu Giu Phe Phe Gin Ile 1 40 Ilie Lys Arg Gin Val Asp Leu Gly 55 Tyr Gly His Val Thr Ser Gly Ala I tac tcc ttc acc Tyr Ser Phe Thr tgg gca agc cag Trp Ala Ser Gin 100 acc agc ttc tct Thr Ser Phe Ser 115 gag gat cca gta Glu Asp Pro Val 130 cca gag ttc acc Pro Giu Phe Thr 145 acg gat gtt gat Thr Asp Val Asp gac ggc ttc gtt Asp Gly Phe Val 180 aac aag ttc tac Asn Lys Phe Tyr 195 gcg ctt tcc cag Ala Leu Ser Gin 210 cag ctc gac gca Gin Leu Asp Ala His Vai Giy Ser Leu Pro Arg 10 Lys Arg Ser Asn Gly Giu Ile Gin Ser Ser Val ASP ASP Val Asp Ile Leu Asn Glu Gly Glu Asp Phe Gly Ala Trp Trp Asn 1020 WO 01/00843 70 Tyr Ser Phe Thr Arg Leu Gly Gly Trp Ala Ser Gin Giu Ala Val Arg 100 Thr Ser Phe Ser Asp Arg Arg Asp 115 120 Glu Asp Pro Val Ser Gly Ile Phe 130 135 Pro Giu Phe Thr Gly Pro Ile Thr 145 150 Thr Asp Val Asp Leu Leu Lys Lys 165 Asp Gly Phe Vai Ala Ala Leu Ser 180 Asn Lys Phe Tyr Asp Thr Asp Giu 195 200 Ala Leu Ser Gin Giu Tyr Lys Ile 210 215 Gin Leu Asp Ala 225 <210> 725 <211> 551 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> <223> RXS02197 <400> 725 gcc gaa cgc atg cgc ttt agc ttcc Ala Glu Arg Met Arg Phe Ser Phe 1 5 ttg tgc atc gcg gat ttc att cgc Leu Cys Ilie Ala Asp Phe Ile Arg ggc caa gtg gac gtc atg cca ttc Gly Gin Val Asp Val Met Pro Phe( 40 att gct gat ttc gcc aac gag ttg Ilie Ala Asp Phe Ala Asn Glu Leu 55 tac ttg gaa gtt cac ggc atc ggc eu ;er

LOS

krg Phr r'yr fly ?ro

LBS

flu Ile :cac ?ro ~ca ?ro2 ~ag ;lnI :tc ?he ;tg 1021 ['hr 90 ['hr kl a fly Ilie 4et 170 fly /a 1 ['hr ~gc krg -gc krg :tg eu Ica kla ~ag 75 Met Pro Leu Arg Gly 155 Asn Ser Val1 Asp cag Gin gag Glu gtc Val1 gcc Ala ctc Thr Gly Phe Ala 140 Gin Ala Al a Al a Ala 220 cag Gln caa Gin acc Thr aat Asn acc Asp Asn Ser 125 Ser Glu Ala Ala Ala 205 Gly cgc Arg gc t Ala atg Met gaa Glu gaa PCTIBOO/00923 sp Arg lu Leu la Tyr ly Asn hr Gin 160 la Thr eu Thr la Asp hr Val 48 96 144 192 240 WO 01/00843 Tyr Leu Glu Val His Gly Ile Gly Val 70 gag tac tgg cac tcc cga gtg cgc agc Giu Tyr Trp His Ser Arg Val Arg *Ser gga tct gtc gct gat ttt gat cca gaa Gly Ser Val Ala Asp Phe Asp Pro Glu 100 105 ctg gat tac cgc ggc gcc cgc ttc tcc Leu Asp Tyr Arg Gly Ala Arg Phe Ser 115 120 gat ctg gaa gac cgc gca aag ctg gtg Asp Leu Giu Asp Arg Ala Lys Leu Val 130 135 ato ggc gtg gag ttg tcc gag gaa ctc Ile Gly Val Giu Leu Ser Glu Giu Leu 145 150 aca gac gcg ttt gtg ctc tac cac cca Thr Asp Ala Phe Val Leu Tyr His Pro 165 taacaccttt gagagggaaa act <210> 726 <211> 176 <212> PRT <213> Corynebacterium giutamicun <400> 726 Ala Glu Arg Met Arg Phe Ser Phe Pro 1 5 Leu Cys Ile Ala Asp Phe Ile Arg Pro 25 Gly Gin Val Asp Val Met Pro Phe Gin 40 Ile Ala Asp Phe Ala Asn Giu Leu Phe 55 Tyr Leu Glu Val His Gly Ile Gly Val 70 Giu Tyr Trp His Ser Arg Vai Arg Ser Gly Ser Val Ala Asp Phe Asp Pro Giu 100 105 Leu Asp Tyr Arg Gly Ala Arg Phe Ser 115 120 Asp Leu Glu Asp Arg Ala Lys Leu Val Gin gaa Giu 90 gac Asp ttt Phe gaa Giu cag Gin gag Giu 170 Leu 75 ctc Leu aag Lys ggt Gly ttg Leu ctg Leu 155 gca Ala Thr aag Lys acc Thr tac Tyr ctc Leu 140 cac His aag Lys Glu ctg Leu aag Lys ggt Gly 125 gag Gi u cca Pro tac Tyr Ala aac Asn ttc Phe 110 tct Ser cca Pro gag Giu ttt Phe PCTIBOO/00923 Leu Ala gac ggt 288 Asp Gly ttc gac 336 Phe Asp tgc cct 384 Cys Pro ggc cgt 432 Gly Arg cag tcc 480 Gin Ser 160 aac gtc 528 Asn Val 175 Gin Giu Val Ala Leu 75 Leu Lys Gly Leu Gin Gin Thr Asn Thr Lys Thr Tyr Leu Arg Ala Met Glu Glu Leu Lys Giy 125 Giu Gly Val1 Giy Tyr Al a Asn Phe 110 Ser Pro Arg Lys Asn Arg Leu Asp Phe Cys Gly Phe Asp Pro Glu Ala Giy Asp Pro Arg 1022 WO 01/00843 WO 0100843PCT/IBOO/00923 130 135 Ile Gly Val Giu Leu Ser Giu Giu Leu Gin Leu 145 150 155 Thr ASP Ala Phe Val Leu Tyr His Pro Giu Ala 165 170 His Pro Glu Gin Lys Tyr Phe Asn Val 175 <210> <211> <212> <213> <220> <221> <222> <223> 727 546

DNA

Corynebacterium giutani cui

CDS

(101) (523) RXCO 0988 <400> 727 tagcagaaga caccgatgta cagtccggat caggtgttgt gatcaccggt tcaatcgtga ccgccggcga tgcgcgcacg ctgtttggaa aggaacctgc atg agc aag cgt Met Ser Lys Arg 1 gaa tca att gag Glu Ser Ile Glu gga cca tta ggc Gly Pro Leu Gly ggc cac gat cca Gly His Asp Pro tta aag Leu Lys gat ccc atg Asp Pro Met gaa gca atc Glu Ala Ilie ggt atc cga ggt Gly Ile Arg Gly atg gcc ggc acc Met Ala Gly Thr tta gtg atg Leu Val Met cgc gtg gac Arg Val Asp acc tta ggt ctt Thr Leu Gly Leu ctc acc gtg att Leu Thr Val Ile gac ggc Asp Gly atc tac tgg acc Ile Tyr Trp Thr ttc aac tgg gtc Phe Asn Trp Val gta tca gca gtc Val Ser Ala Val atc gca cac ttt Ile Ala His Phe gt t Val1 gct gca ttt ctg Ala Ala Phe Leu agg ttt agt tgg Arg Phe Ser Trp atc ccg atg aac Ile Pro Met Asn gtg ctg cag gtt ctt gca ctt gcc ggt Val Leu Gin Val Leu Ala Leu Ala Gly ttc ttt Phe Phe 100 gtt cac ccc Val His Pro gcg tac ctg Ala Tyr Leu 120 atg ggc ttc gcc Met Gly Phe Ala atc atc ttc atc Ile Ile Phe Ile atc gcg tgg Ile Ala Trp, 115 atg aaa cgc Met Lys Arg ttc tac ctg cgc tct aat ctg att gat Phe Tyr Leu Arg Ser Asn Leu Ilie Asp 125 1023 WO 01/00843 PCTILBOO/00923 ggg ctg ctt acc acg cag cac agc taagctttaa ggccctccgg ggc 546 Giy Leu Leu Thr Thr Gin His Ser 135 140 <210> 728 <211> 141 <212> PRT <213> Corynebacteriun giutainicum <400> 728 Met Ser Lys Arg Glu Giu Ser Ile Giu Tyr Gly Pro Leu Gly Lys Giy 1 5 10 His Asp Pro Leu Lys Asp Pro Met Lys Gly Ile Arg Gly Val Met Ala 25 Giy Thr Leu Val Met Giu Ala Ile Thr Leu Gly Leu Val Leu Thr Val 40 Ilie Leu Arg Val Asp Asp Gly Ile Tyr Trp Thr Thr Phe Asn Trp Val 55 Tyr Vai Ser Aia Vai Ala Ile Ala His Phe Val Ala Ala Phe Leu Gin 70 75 Arg Phe Ser Trp, Ser Ile Pro Met Asn Ile Val Leu Gin Val Leu Ala 90 Leu Ala Gly Phe Phe Val His Pro Ala Met Gly Phe Ala Ala Ile Ile 100 105 110 Phe Ile Ilie Ala Trp, Ala Tyr Leu Phe Tyr Leu Arg Ser Asn Leu Ile 115 120 125 Asp Arg Met Lys Arg Giy Leu Leu Thr Thr Gin His Ser 130 135 140 <210> 729 <211> 597 <212> DNA <213> Corynebacterium glutanicumr <220> <221> CDS <222> (101)..(574) <223> RXCO1518 <400> 729 agcagaacct gatgccgtcc tgcacggcac gaccattgca gaacatgtgg ataatcttga tcccacagac attgaaggtg tcaccaagat ttaaggagtc gtg gct ttc atg cag 115 Val Ala Phe Met Gin 1 aaa acg tca gcg ggt tgg ttg atc gca acg gga ggt ttc ctc gcc gct 163 Lys Thr Ser Ala Gly Trp Leu Ile Ala Thr Gly Gly Phe Leu Ala Ala 15 gtg tcc gcc att ttg acg tgg cgt ttt tat gga tcc atg acc tct att 211 1024 WO 01/00843 Val Ser Ala tcc atc acg Ser Ilie Thr ttc gca ggt Phe Ala Gly cag gac aaa Gin Asp Lys ctg ggt cga Leu Gly Arg gtg gga att Val Gly Ilie gca tcg aat Ala Ser Asn 120 gca ctc tca Ala Leu Ser 135 Leu tcc Ser aag Lys caa Gin tg t Cys agt Ser ctt Leu gcc Ala Thr a tc Ile gtc Val atg Met 75 gcg Aia tat Tyr ccg Pro gga G iy Tyr Giy Ser Met ttg ttg gcc gtg Leu Leu Ala Val ctc gat gag ggg Leu Asp Giu Giy acc att gcc tat Thr Ile Ala Tyr 80 gca att ttc ggc Ala Ile Phe Gly 95 cgc gcc ggt gag Arg Ala Gly Glu gcc tgt gcg ctg Ala Cys Ala Leu 130 gag cga agc tgt Giu Arg Ser Cys 145 PCTJIBOOIOO923 Thr Ser Ile gtg tgt ggt 259 Val Cys Gly ctg atc ggc 307 Leu Ile Gly ctg gcc atg 355 Leu Ala Met ggc gtt tat 403 Giy Val Tyr 100 ttg tcc gca 451 Leu Ser Ala 115 ggc gga atc 499 Giy Giy le gag gct ccg 547 Glu Ala Pro cct ccc caa tct ggc gaa gcg atc agc Pro Pro Gin Ser Giy Giu Ala Ile Ser 150 155 aag <210> 730 <211> 158 <212> PRT <213> Corynebacterium giutanicun <400> 730 Val Ala Phe Met Gin Lys Thr Ser Ala 1 Gly Phe Leu Ala Ala Val Ser Ala Ile Ser Met Thr Ser Ile Ser Ile Thr Val 40 Ala Val Val Cys Gly Phe Ala Gly Val Giu Gly Leu Ile Gly Gin Asp Lys Ser Ala Tyr Leu Ala Met Leu Gly Arg Ala tagattggaa ttcatgaatc Ile Arg Phe Gly Pro Gly 1025 WO 01/00843 WO 0100843PCTIIBOO/00923 Phe Gly Gly Val Tyr Val Gly Ile Gly Ser Tyr Val Ile Pro Arg Ala 100 105 110 Gly Glu Leu Ser Ala Ala Ser Asn Asp Leu Pro Giy Val Ile Ala Cys 115 120 125 Ala Leu Gly Gly Ile Ala Leu Ser Ala Ala Gly Leu Tyr Leu Giu Arg 130 135 140 Ser Cys Glu Ala Pro Pro Pro Gin Ser Gly Giu Ala Ile Ser 145 150 155 <210> 731 <211> 723 <212> DNA <213> Corynebacteriun giutamicun <220> <221> CDS <222> (101)..(700) <223> RXC01942 <400> 731 gccgcgaaat tcggtgaaat tgaaggtatt cctgcagatc aggcaaattc ttccacga ct gtgatcaagg tcaacggcaa gaacgagtaa cctgggatcc ac a Thr tct Ser gtC Val caa Gin gcc Ala acc Thr gaa Glu gaa Glu gga Gly gaa Giu gaa Giu gac Asp aa a Lys cac His ga t Asp aaa Lys ggg Gly gga Gly ccc Pro atc Ile gca Al a cct Pro caa Gin 105 ggc Gly tct Ser gcg Ala gca Ala act Thr caa Gin tca Ser gtt Val1 gac Asp acc Thr gac Asp gag Giu cta Leu aca Thr gct Ala tat Tyr ctt Leu atg ttg cgc att gga 115 Met Leu Arg Ile Gly 1 gtt gcc gat ctt. ttg 163 Val Ala ASP Leu Leu caa gtt gcc cgc gat 211 Gin Val Ala Arg Asp cta gct gaa gct ttt 259 Leu Ala Giu Ala Phe gac cgc gcg gga tta 307 Asp Arg Ala Gly Leu gcg ctg ctc aat gcc 355 Ala Leu Leu Asn Ala cgt cga ttc aac gaa 403 Arg Arg Phe Asn Giu 100 gac atg cct ttg ctt 451 Asp Met Pro Leu Leu 115 gtc gtc gta gtt gat 499 Val Val1Val 1026 WO 01/00843 WO 0100843PCTTBOO/00923 gac Asp 135 gag Giu aga Arg gac Asp 120 125 gta gag gaa cgc gtc cgc aga ctt Val Giu Giu Arg Val Arg Arg Leu 140 gac gac gtg cgg cgt cga atc gct Asp Asp Val Arg Arg Arg Ile Ala 155 ctt aaa gcc gct gac atc gtt gtg Leu Lys Ala Ala Asp Ile Val Val 170 175 ctt cat gct gaa gca agc aag ctg Leu His Ala Glu Ala Ser Lys Leu 185 190 aat tagcactaaa acatcgtcaa agt gtg Val1 tct Ser 160 gac Asp att Ile cgt Arg ccc Pro ggc Gly att Ile 195 cgC gtg Arg Val Asn 200 <210> 732 <211> 200 <212> PRT <213> Corynebacterium glutamicun <400> 732 Met Leu Arg Ilie 1 Val Ala Asp Leu Gin Val Ala Arg Leu Ala Giu Ala Asp Arg Ala Gly Ala Leu Leu Asn Arg Arg Phe Asn 100 Asp Met Pro Leu 115 Val Val Val Val 130 Giu Lys Arg Gly 145 Gin Val Pro Aso Leu Thr Gly Gly Ser Ser Giu Gly 25 Ile Val GJlu Pro Gly Gin Asp Ile 55 Ala Ala Lys Ala 70 Ile Thr His Pro Ala Giu Asp Gin 105 Val Giu Lys Gly 120 Val Asp Val Giu 135 Thr Giu Asp Asp 150 Val Arg Leu Lys Ile 10 Phe Gly Leu Phe Arg Gly Leu Giu Val1 Ala 170 Gly Leu Gin Lys Val 75 Ile Ala Asp Arg Arg 155 Ala Ser Ile Pro Pro Ser Ala Lys Arg Val 140 Arg Asp Lys Asp Leu Gly Giu Glu Ala 110 Met Arg Ile Val1 Ser Ala Ala Thr Gin Ser Val Asp Leu Ala Val1 175 Thr Asp Giu Leu Thr Ala Tyr Leu Val1 Ser 160 Asp 1027 WO 01/00843 WO 0100843PCTIBOO/00923 Asn Asn Gly Thr Leu Glu Asp Leu His Ala Glu Ala Ser Lys Leu Ile 180 185 190 Ala Giu Ilie Leu Ser Arg Val Asn 195 200 <210> 733 <211> 1194 <212> DNA <213> Corynebacteriun g lutamicum <220> <221> <222> <223>

CDS

(101)..(1171) RXN0 2802 <400> 733 ccttcgccgc ctgctccgac ctcgccgacg ccgtcaaagc ccaggtcccg atctggaaag agcaaacgcg cctcgacggc tccaccgatt gggtcggcct gtg aaa aac ctc Val Lys Asn Leu atc gcc cgc tac Ile Ala Arg Tyr cgc Arg cgc caa att atg ctc ggc gaa atc ggc Arg Gin Ile Met Leu Gly Glu Ile Gly 15 cag caa Gin Gin aaa caa caa Lys Gin Gin ggc ctc ggg Giy Leu Giy tcg Ser ctt ttc gac gct Leu Phe Asp Ala gtc tcc gtc atc Val Ser Val Ile ggc gca ggc Gly Ala Gly ggc gtc ggc Gly Val Gly tca ccc gcc ctg Ser Pro Ala Leu tac ctt gct ggc Tyr Leu Ala Gly gct Ala cac atc cac atc att gac His Ilie His Ilie Ile Asp gac ctc gtc gac Asp Leu Val Asp tcc aac ctc cac Ser Asn Leu His cag gtc att cac Gin Val Ile His acc gct ggc gtt Thr Ala Giy Val aca ccc aag gcc Thr Pro Lys Ala tcc gcg cgc gaa Ser Ala Arg Glu gca Ala atg ctg gca ctg Met Leu Ala Leu cct tcc gtt aaa Pro Ser Val Lys gtg acg Va.l Thr 100 gtt tct gtc Val Ser Val gat tcc gat Asp Ser Asp 120 cga ctg gac tgg tca aat gca ctt tct Arg Leu Asp Trp Ser Asn Ala Leu Ser 110 gag ctg gca Glu Leu Ala 115 acc cga cac Thr Arg His gtg att ttg gat Val Ile Leu Asp tcc gat aac ttc Ser Asp Asn Phe gac Asp 130 ctc gca tcc tgg gcc gcc Leu Ala Ser Trp, Ala Ala 135 aaa ctt ggc atc Lys Leu Gly Ile cac gtc tgg gca His Val Trp Ala 1028 WO 01/00843 tcc atc ctg Ser Ile Leu 150 ggc ccc atc Giy Pro Ile gtc cca tca Val Pro Ser atg ggc tcc Met Gly Ser 200 ggc aca ccc Gly Thr Pro 215 acc tgg gaa Thr Trp Giu 230 gtg ctt ggg Vai Leu Gly gtg ctc gat Vai Leu Asp atc gac gtc Ile Asp Val 280 gcg cac aac 1027 Ala His Asn 295 tcc gtt tcc 1075 Ser Val Ser 310 cgc tcc gca 1123 Arg Ser Ala atg agc agc 1171 gac Asp 155 gac Asp caa Gin gcc Ala gga Gly ccc Pro 235 ggt Gly cga Arg ccc Pro c tg Leu aaa Lys 315 atc Ile ggc c aa Gin ttc Phe ggc Gly gaa Giu 205 c tc Leu gtc Vai tcg Ser tcc Ser gaa Giu 285 gcc Al a gtt Val1 at t Ile atc Ile ctc Leu ccc Pro gtt Va 1 190 gcc Al a ggc Gly ggt Giy ggg Gly gcg Ala 270 ttc Phe atc Ile atc Ile tta Leu gaa Giu gtc Val1 160 ccg Pro ggt Gly aaa Lys t ac Tyr ccg Pro 240 tct -Ser gtt Val1 gcc Ala gaa Giu tac Tyr 320 tcc Ser tgg Trp cac His cca Pro gtt Val atc Ile 210 tcc Ser gtg Val ggt Gly ggC Gly tcc Ser 290 gcc Ala gca Ala ggC Gly gat Asp gcc Ala ccc Pro gtc Val 195 acc Thr c tc Leu ctg Leu ttt Phe gtt Val1 275 atc Ile atc Ile gc t Ala tac Tyr tcc Ser 355 PCTIBOO/00923 ggc cac 595 Gly His 165 gga tcc 643 Gly Ser 180 ggc gta 691 Gly Val ggc gtg 739 Gly Val gac ggc 787 Asp Gly gaa cgg 835 Giu Arg 245 ggt gag 883 Gly Giu 260 tcg ctc 931 Ser Leu ccc ggc 979 Pro Gly cca ccc Pro Pro ggt gtc Gly Val 325 acc gga Thr Gly 340 cta ggg Leu Gly Met Ser Ser Leu Asp Gly Gly 345 taaaaccaag gcgttgtgcc acc 1194 <210> 734 1029 WO 01/00843 PCTIiBOO/00923 <211> 357 <212> PRT <213> Corynebacteriun glutamicumr <400> 734 Val Lys Asn Leu Asp Ile Ala Arg Tyr Arg Arg Gin Ile Met Leu Gly 1 5 10 Glu Ilie Gly Gin Gin Lys Gin Gin Ser Leu Phe Asp Ala Lys Val Ser 25 Val Ilie Giy Ala Gly Gly Leu Giy Ser Pro Ala Leu Leu Tyr Leu Ala 40 Gly Ala Gly Val Giy His Ile His Ile Ile Asp Asp Asp Leu Val Asp 55 Leu Ser Asn Leu His Arg Gin Val Ile His Thr Thr Ala Gly Val Gly 70 75 Thr Pro Lys Ala Glu Ser Ala Arg Glu Ala Met Leu Ala Leu Asn Pro 90 Ser Val Lys Val Thr Val Ser Val Arg Arg Leu Asp Trp Ser Asn Ala 100 105 110 Leu Ser Glu Leu Ala Asp Ser Asp Val Ile Leu Asp Gly Ser Asp Asn 115 120 125 Phe Asp Thr Arg His Leu Aia Ser Trp Ala Ala Ala Lys Leu Gly Ile 130 135 140 Pro His Val Trp Ala Ser Ile Leu Giy Phe Asp Ala Gin Leu Ser Val 145 150 155 .160 Phe His Ala Gly His Gly Pro Ile Tyr Glu Asp Leu Phe Pro Thr Pro 165 170 175 Pro Pro Pro Gly Ser Val Pro Ser Cys Ser Gin Ala Gly Val Leu-Gly 180 185 190 Pro Val Val Gly Val Met Gly Ser Ala Met Ala Met Glu Ala Leu Lys 195 200 205 Ile Ile Thr Gly Val Gly Thr Pro Leu Ile Gly Lys Leu Gly Tyr Tyr 210 215 220 Ser Ser Leu Asp Gly Thr Trp Glu Tyr Ile Pro Val Val Gly Ser Pro 225 230 235 240 Glu Val Leu Glu Arg Val Leu Gly Ser Ala Gly Vai Ser Gly Ile Ser 245 250 255 Gly Gly Phe Gly Glu Val Leu Asp Val Pro Arg Val Ser Ala Leu Val 260 265 270 Asp Gly Val Ser Leu Ile Asp Val Arg Glu Pro Ser Glu Phe Ser Ala 275 280 285 Tyr Ser Ile Pro Gly Ala His Asn Thr Pro Leu Ser Ala Ile Arg Glu 290 295 300 1030 WO 01/00843 WO 0100843PCT/IBOO/00923 Gly Ala Ile Pro Pro Ser 305 310 Val Ser Ala Gly Lys 315 Glu Val Ile Vai Cys Ala Ala Gly Val1 325 Arg Ser Ala Gin Ala 330 Ile Ala Ile Leu Giu Ser 335 Ala Gly Tyr Thr 340 Gly Met Ser Ser Asp Gly Gly Ile Giu Gly Trp 350 Leu Asp Ser Leu Gly 355 <210> 735 <211> 497 <212> DNA <213> Corynebacterium giutamicun <220> <221> <222> <223>

CDS

(474) FRXA02 802 <400> 735 tccgcgatg gcc atg gaa Met Ala Met Giu 1 gcc ctg aaa atc atc acc ggc gtg ggc aca ccc 51 Ala Leu Lys Ile Ile Thr Gly Val Gly Thr Pro ttg atc gga aaa Leu Ile Gly Lys ggc tac tac tcc Gly Tyr Tyr Ser ctc gac ggc acc Leu Asp Gly Thr tgg gaa Trp Giu, tac atc ccc Tyr Ilie Pro tct gct ggt Ser Ala Gly gtc ggt tcg ccg gag gtg ctg gaa cgg Val Gly Ser Pro Giu Val Leu Giu Arg gtg ctt ggg Val Leu Giy gtg ctc gat Val Leu Asp gtt tcg ggg att Val Ser Gly Ilie ggc ggt ttt ggt Gly Gly Phe Gly gag Glu 195 gtt cct Val Pro cga gtt tcc gcg Arg Val Ser Ala gtt gac ggc gtt Val Asp Gly Val ctc atc gac gtc Leu Ile Asp Vai cgc Arg gaa CCC tcc gaa Giu Pro Ser Glu ttc Phe tcc gcc tac tcc Ser Ala Tyr Ser ccc ggc gcg cac Pro Gly Ala His acc cca ctg tcc Thr Pro Leu Ser atc cgc gaa ggc gcc atc cca ccc tcc Ile Arg Giu Gly Ala Ile Pro Pro Ser 105 gtt tcc Val Ser 110 339 gca ggt aaa Ala Giy Lys caa gcc atc Gin Ala Ile 130 gtt atc gtc tac Val Ile Val Tyr gca gct ggt gtc Ala Ala Gly Val cgc tcc gca Arg Ser Ala 125 atg agc agc Met Ser Ser gca att tta gaa tcc gca ggc tac acc Ala Ile Leu Giu Ser Ala Gly Tyr Thr 135 gga Gly 140 WO 01/00843 PCTIBOOIOO923 ctc gac ggc gga atc gaa ggc tgg cta gat tcc cta ggg taaaaccaag 484 Leu Asp Gly Gly Ile Glu Gly Trp Leu Asp Ser Leu Gly 145 150 155 gcgttgtgcc acc 497 <210> 736 <211> 156 <212> PRT <213> Corynebacterium giutamicum <400> 736 Met Ala Met Glu Ala Leu Lys Ile Ile Thr Gly Val Gly Thr Pro Leu 1 5 10 Ile Gly Lys Leu Gly Tyr Tyr Ser Ser Leu Asp Gly Thr Trp Glu Tyr 25 Ilie Pro Val Val Gly Ser Pro Glu Val Leu Glu Arg Val Leu Gly Ser 40 Ala Gly Val Ser Gly Ile Ser Gly Gly Phe Gly Glu Val Leu Asp Val 55 Pro Arg Val Ser Ala Leu Val Asp Gly Val Ser Leu Ile Asp Val Arg 70 75 Glu Pro Ser Glu Phe Ser Ala Tyr Ser Ile Pro Gly Ala His Asn Thr 90 Pro Leu Ser Ala Ile Arg Glu Gly Ala Ile Pro Pro Ser Val Ser Ala 100 105 110 Giy Lys Glu Vai Ile Val Tyr Cys Ala Ala Giy Val Arg Ser Ala Gin 115 120 125 Ala Ilie Ala Ile Leu Glu Ser Ala Gly Tyr Thr Gly Met Ser Ser Leu 130 135 140 Asp Gly Giy Ile Glu Gly Trp Leu Asp Ser Leu Gly 145 150 155 <210> 737 <211> 535 <212> DNA <213> Corynebacteriun glutamicun <220> <221> CDS <222> (101)..(535) <223> FRXA00438 <400> 737 ccttcgccgc ctgctccgac ctcgccgacg ccgtcaaagc ccaggtcccg atctggaaag agcaaacgcg cctcgacggc tccaccgatt gggtcggcct gtg aaa aac ctc gac 115 Val Lys Asn Leu Asp 1 1032 WO 01/00843 atc gcc cgc tac cgc cgc caa att Ilie Ala Arg Tyr Arg Arg Gin IleI aaa caa caa tcg ctt ttc gac gct Lys Gin Gin Ser Leu Phe Asp Ala ggc ctc ggg tca ccc gcc ctg ctc I Gly Leu Gly Ser Pro Ala Leu Leu 45 cac atc cac atc att gac gat gac His Ile His Ile Ile Asp Asp Asp 1 60 cgc cag gtc att cac acc acc gct5 Arg Gin Val Ilie His Thr Thr Ala 75 tcc gcg cgc gaa gca atg ctg gca Ser Ala Arg Giu Ala Met Leu Ala gtt tct gtc agg cga ctg gac tgg I Vai Ser Val Arg Arg Leu Asp Trp 105 gat tcc gat gtg att ttg gat ggcI Asp Ser Asp Val Ilie Leu Asp Gly 120 125 ctc gca tcc tgg gcc gcc gca aaa Leu Ala Ser Trp Ala Ala Ala Lys 135 140 <210> 738 <211> 145 <212> PRT <213> Corynebacteriun giutanicuni <400> 738 Val Lys Asn Leu Asp Ilie Ala Arg 1 Giu Ile Gly Gin Gin Lys Gin Gin Val Ilie Gly Ala Gly Gly Leu Gly Gly Ala Gly Val Gly His Ile His 55 Leu Ser Asn Leu His Arg Gin Val Thr Pro Lys Ala Glu Ser Ala Arg gaa Glu gtc Val ggc Gly ctc Leu aca Thr tcc Ser ctt Leu ttc Phe ccc Pro 145 Gin Asp Leu Asp Thr Leu atc Ile atc Ile gct Ala tcc Ser ccc Pro gt t Val tct Ser gac Asp 130 PCTIiBOO/00923 ag caa 163 Ln Gin :a ggc 2ii La Gly -c ggc 259 il Gly :c cac 307 au His c gag 355 la Giu :g acg 403 al Thr )o :g gca 451 !u Ala ja cac 499 r-g His 1033 WO 01/00843 PCTIBOOIOO923 Ser Val Lys Val Thr Val Ser Val Arg Arg Leu Asp Trp Ser Asn Ala 100 105 110 Leu Ser Glu Leu Ala Asp Ser Asp Val Ile Leu Asp Gly Ser Asp Asn 115 120 125 Phe Asp Thr Arg His Leu Ala Ser Trp Ala Ala Ala Lys Leu Gly Ile 130 135 140 Pro 145 <210> 739 <211> 579 <212> DNA <213> Corynebacteriun glutamicun <22 0> <221> CDS <222> (101)..(556) <223> RXN00437 <400> 739 ttcatcatgg cgctgcccgg ctccacgggt gcggcgcgcg atgccaccgc tgtcctcgac ccactcattg atcacatcac tggaactctg caaggccacc atg aac act Met Asn Thr gct Ala acc Thr aca Thr cac His acc Thr cac His atc Ile gac Asp gaa Glu gaa Glu ggC Gly gcc Ala gtc Val cgc Arg gcc Ala gcc Al a caa Gln ccg Pro 9c9 Ala cgc Arg ctt Leu 75 ctc Leu ttc Phe t gc Cys ggc Gly C tC ILeu gtc Val acc Thr gct Ala acc Thr gtc Val gac Asp 125 aaa Lys gac Asp acc Thr t cc Ser gtc Val gcg Ala gtc Val 110 C tC Leu 1 gac Asp aaa Lys ggc Gly t ac Tyr tcc Ser acc Thr t cc Ser git t Ala aac Asn atc Ile acc Thr atc Ile ggc Gly gcc Ala ttc Phe ggc Gly gtc Val1 9c9 Al a gt t Val1 gcc Ala cac His 115 gac ccc Asp Pro ctc acc Leu Thr gtc tgc Val Cys cgc gac Arg Asp cat ccc His Pro gaa aaa Glu Lys ttg aaa Leu Lys 100 cgc gcc Arg Ala 115 163 211 259 307 355 403 451 499 gcc gac gcc gtc aaa gcc cag Ala Asp Ala Val Lys Ala Gin 130 1034 WO 01/00843 WO 0100843PCT/EB00100923 gtc ccg atc tgg aaa gag caa acg cgc ctc gac ggc tcc acc gat tgg 547 Val Pro Ile Trp Lys Giu Gin Thr Arg Leu Asp Gly Ser Thr Asp Trp 135 140 145 gtc ggc ctg tgaaaaacct cgacatcgcc cgc 579 Vai Gly Leu 150 <210> 740 <211> i52 <2i2> PRT <213> Corynebacteriun giutanicun <400> 740 Met Asn Thr Asp Pro Ala Tyr Val Ala Giu Gin Thr Gly Lys Leu Ile 1 5 10 Asp Ala Phe Leu Thr Thr Asp Pro Leu Giu Pro Leu Leu Asp Ala Ala 25 Lys Asn Gly Val Cys Thr Giu Ala Met Gly Ala Leu Val Thr Phe Giu 40 Gly Ile Val Arg Asp His Asp Gly Giy Ala Arg Val Thr Ser Leu Thr 55 Tyr Thr Ala His Pro Thr Ala Pro Gin Val Leu Ser Ala Val Ala Asp 70 75 Ser Ile Val Giu Lys His Pro Arg Thr Arg Leu Trp, Thr Ala His Arg 90 Thr Gly Ala Leu Lys Ile Giy Asp Ala Ala Phe Leu Val Val Ala Ala 100 105 110 Ser Ala His Arg Ala Asp Ala Phe Ala Ala Cys Ser Asp Leu Ala Asp 115 120 125 Ala Val Lys Ala Gin Val Pro Ile Trp Lys Glu Gin Thr Arg Leu Asp 130 135 140 Gly Ser Thr Asp Trp Val Gly Leu 145 150 <210> 741 <211> 383 <212> DNA <213> Corynebacteriun giutamicun <22 0> <221> CDS <222> <223> FRXA00437 <400> 741 aaa aac ggc gtc tgc aca gag gcg atg ggc gcg ctg gtc. acc ttc gaa 48 Lys Asn Gly Val Cys Thr Giu Ala Met Gly Ala Leu Val Thr Phe Giu 1 5 10 1035 WO 01/00843 WO 0100843PCTI~IBOO/00923 ggc atc gtc cgc gac cac gac ggc ggc gcc cgc gtg acc tcc ctg acc 96 Gly Ile Val Arg Asp His Asp Gly Gly Ala Arg Val Thr Ser Leu Thr 25 tac acc gcg cat ccc acc gcg ccg cag gtc ctt tct gct gtc gcg gac 144 Tyr Thr Ala His Pro Thr Ala Pro Gin Val Leu Ser Ala Val Ala Asp 40 tcc atc gtt gaa aaa cac ccg cgc acc cgc ctc tgg acc gcg cac cgc 192 Ser Ile Val Glu Lys His Pro Arg Thr Arg Leu Trp Thr Ala His Arg 55 acc ggc gcc ttg aaa atc ggt gac gcc gcc ttc ctc gtc gtc gcc gcc 240 Thr Gly Ala Leu Lys Ile Gly Asp Ala Ala Phe Leu Val Val Ala Ala 70 75 tcc gcc cac cgc gcc gac gcc ttc gcc gcc tgc tcc gac ctc gcc gac 288 Ser Ala His Arg Ala Asp Ala Phe Ala Ala Cys Ser Asp Leu Ala Asp 90 gcc gtc aaa gcc cag gtc ccg atc tgg aaa gag caa acg cgc ctc gac 336 Ala Val Lys Ala Gin Val Pro Ilie Trp Lys Giu Gin Thr Arg Leu Asp 100 105 110 ggc tcc acc gat tgg gtc ggc ctg tgaaaaacct cgacatcgcc cgc 383 Gly Ser Thr Asp Trp Val Gly Leu 115 120 <210> 742 <211> 120 <212> PRT <213> Corynebacterium giutanicum <400> 742 Lys Asn Gly Val Cys Thr Glu Ala Met Gly Ala Leu Val Thr Phe Glu 1 5 10 Gly Ilie Val Arg Asp His Asp Gly Gly Ala Arg Val Thr Ser Leu Thr 25 Tyr Thr Ala His Pro Thr Ala Pro Gin Val Leu Ser Ala Val Ala Asp 40 Ser Ilie Val Giu Lys His Pro Arg Thr Arg Leu Trp Thr Ala His Arg 55 Thr Gly Ala Leu Lys Ile Gly Asp Ala Ala Phe Leu Val Val Ala Ala 70 75 Ser Ala His Arg Ala Asp Ala Phe Ala Ala Cys Ser Asp Leu Ala Asp 90 Ala Val Lys Ala Gin Val Pro Ilie Trp Lys Giu Gin Thr Arg Leu Asp 100 105 110 Gly Ser Thr Asp Trp Val Gly Leu 115 120 1036 WO 01/00843 WO 0100843PCT/IBOO/00923 <210> <211> <212> <213> <220> <221> <222> <223> 743 591

DNA

Corynebacteriun glut aiic ur

CDS

(101)..(568) RXN00439 <400> 743 ctgccaccgc agactgtctg atcaggatcc cggcgcggac tacggtggag gaaaacgaca tcgttaagat ttacccattc aactaacagg agttaattta atg agc gag ctc Met Ser Giu Leu 115 163 cac gtt His Val cga gca gac ggt tcc gca cat atg gtg gat gtg acg ggc aaa Arg Ala Asp Gly Ser Ala His Met Val Asp Val Thr Gly Lys 15 aat gaaaca tcg aga act gct gtt Asn Giu Thr Ser Arg Thr Ala Val ggg gac gtc gta aag cag ctt ttt Gly Asp Val Val Lys Gin Leu Phe gcc Al a 30 gaa ggg ttt gtg Giu Gly Phe Val aag atg agg Lys Met Arg aaa ggc gac Lys Gly Asp agt gct ggt ctg Ser Ala Gly Leu gcg cta cct gtg gcg cgg att gcg ggt atc atg ggt gcg aag aag acg Ala Leu Pro Val Ala Arg Ile Ala Gly Ile Met Gly Ala Lys Lys Thr 60 gac att atc cct Asp Ile Ile Pro tgc cac ccg ttg Cys His Pro Leu ctg ggc aaa att Leu Gly Lys Ile gtg gat ttt ttt Val Asp Phe Phe ctt act gat ggt Leu Thr Asp Giy cgg att gag gct Arg Ile Glu Ala tcg gtg Ser Val 100 aaa acg cgt Lys Thr Arg agc act gcg Ser Thr Ala 120 ggg Gly 105 gtt act ggt gtg Val Thr Gly Val gaa Glu 110 atg gag gcg ttg Met Glu Ala Leu acg gcc gtg Thr Ala Val 115 gtg gat aag Val Asp Lys gcg ctg acg gta Ala Leu Thr Val gac atg atc aag Asp Met Ile Lys atg gcc Met Ala 135 gtg att gat ggc Val Ile Asp Gly att Ile 140 cgt gtg ctg tcg Arg Val Leu Ser act ggc ggt aaa Thr Gly Gly Lys tc t Ser 150 ggg gat tgg tct Gly Asp Trp Ser gtt cag Val Gin 155 tgacagctct ggttatcgtt gcg <210> 744 <211> 156 <212> PRT 1037 WO 01/00843 PCTIIBOO/00923 <213> Corynebacteriun glutamicuni <400> 744 Met Ser Glu Leu Thr His Val Arg Ala Asp Gly Ser Ala His Met Val 1 5 10 Asp Val Thr Gly Lys Asn Glu Thr Ser Arg Thr Ala Val Ala Glu Gly 25 Phe Val Lys Met Arg Gly Asp Val Val Lys Gln Leu Phe Ser Ala Gly 40 Leu Pro Lys Gly Asp Ala Leu Pro Val Ala Arg Ile Ala Gly Ile Met 55 Gly Ala Lys Lys Thr Pro Asp Ile Ile Pro Leu Cys His Pro Leu Pro 70 75 Leu Gly Lys Ile Thr Val Asp Phe Phe Glu Leu Thr Asp Gly Val Arg 90 Ile Glu Ala Ser Val Lys Thr Arg Gly Val Thr Gly Val Glu Met Glu 100 105 110 Ala Leu Thr Ala Val Ser Thir Ala Ala Leu Thr Val Tyr Asp Met Ile 115 120 125 Lys Ala Val Asp Lys Met Al a Val Ile Asp Gly Ile Arg Val Leu Ser 130 135 140 Lys Thr Gly Gly Lys Ser Gly Asp Trp Ser Val Gln 145 150 155 <210> 745 <211> 218 <212> DNA <213> Corynebacteriun glutamicum <22 0> <221> CDS <222> <223> FR.XA00439 <400> 745 act gat ggt gtt cgg att gag gct tcg gtg aaa acg cgt ggg gtt act 48 Thr Asp Gly Val Arg Ile Glu Ala Ser Val Lys Thr Arg Gly Val Thr 1 5 10 ggt gtg gaa atg gag gcg ttg acg gcc gtg agc act gcg gcg ctg acg 96 Gly Val Glu Met Glu Ala Leu Thr Ala Val Ser Thr Ala Ala Leu Thr 25 gta tac gac atg atc aag gct gtg gat aag atg gcc gtg att gat ggc 144 Val Tyr Asp Met Ilie Lys Ala Val Asp Lys Met Ala Val Ilie Asp Gly 40 att cgt gtg ctg tcg aaa act ggc ggt aaa tct ggg gat tgg tct gtt 192 Ile Arg Val Leu Ser Lys Thr Gly Gly Lys Ser Gly Asp Trp Ser Val 55 1038 WO 01/00843 PCTIIB cag tgacagctct ggttatcgtt gcg Gin <210> 746 <211> <212> PRT <213> Corynebacterium giutamicun <400> 746 Thr Asp Gly Val Arg Ile Giu Ala Ser Val Lys Thr-Arg Gly Val Thr 1 5 10 Giy Vai Giu met Giu Ala Leu Thr Ala Vai Ser Thr Ala Ala Leu Thr 25 Val Tyr Asp Met Ilie Lys Ala Val Asp Lys Met Ala Val Ile Asp Gly 40 Ilie Arg Val Leu Ser Lys Thr Gly Gly Lys Ser Gly Asp Trp Ser Val 55 Gin <210> 747 <211> 358 <212> DNA <213> Corynebacterium giutamicun <22 0> <221> CDS <222> (101)..(358) <223> FRXA00442 <400> 747 ctgccaccgc agactgtctg atcaggatcc cggcgcggac tacggtggag gaaaacga tcgttaagat ttacccattc aactaacagg agttaattta atg agc gag ctc acc 00/00923 218 ca gtt cga Val Arg gaa aca Giu Thr gac gtc Asp Val cta cct Leu Pro gac att gca Ala tcg Ser gta Val1 gt g Val1 atc cat His I gcc Ala 30 agt Serj Gly ccg 1039 Met Ser Glu Leu Thr 1 gat gtg acg ggc aaa Asp Val Thr Gly Lys ttt gtg aag atg agg Phe Val Lys Met Arg ctg cct aaa ggg gac Leu Pro Lys Gly Asp ggt gcg aag aag acg Gly Ala Lys Lys Thr ctg ggc aaa att act WO 01/00843 PCTIIBOO/00923 Pro Asp Ile Ile Pro Leu Cys His Pro Leu Pro Leu Gly Lys Ile Thr 75 80 gtg 358 Val1 <210> 748 <211> 86 <212> PRT <213> Corynebacteriun glutamicuir <400> 748 Met Ser Glu Leu Thr His Val Arg 1 5 Asp Val Thr Gly Lys Asn Giu Thr Phe Val Lys Met Arg Gly Asp Val 40 Leu Pro Lys Giy Asp Ala Leu Pro 55 Gly Ala Lys Lys Thr Pro Asp Ile 70 Leu Gly Lys Ilie Thr Val Ala Asp Gly Ser Ala His Met 10 Ser Arg Thr Ala Val Ala Glu 25 Val Lys Gin Leu Phe Ser Ala Val Ala Arg Ile Ala Gly Ile Ile Pro Leu Cys His Pro Leu 75 Val1 Gly Gly Met Pro <210> 749 <211> 582 <212> DNA <213> Corynebacteriun glutamicun <220> <221> CDS <222> (101)..(559) <223> RXA00440 <400> 749 cggtatacga catgatcaag gctgtggata agatggccgt tgtcgaaaac tggcggtaaa tctggggatt ggtctgttca atc gtt gcg tcc act cgc gcc gct gcc ggg gtg Ile Val Ala Ser Thr Arg Ala Ala Ala Gly Val 15 ggc cca att ttg gtg tcg tgg ctg cgt gca aaa Gly Pro Ile Leu Val Ser Trp Leu Arg Ala Lys 30 gcc ccc gtg atc gtg gcg gac gcc aac ctg ccc Ala Pro Val Ile Val Ala Asp Ala Asn Leu Pro gattgatggc attcgtgtgc gtg aca gct ctg gtt Val Thr Ala Leu Val 1 tat gag gat cgc tct Tyr Glu Asp Arg Ser ggt ttt gac aca ccc Giv Phe Asn Thr Pro gca Ala ctg gac gag Leu Asp Glu 1040 WO 01/00843 WO 0100843PCT/iBOO/00923 ctg gaa ttt ccg cag gta gta ctt Leu Giu Phe Pro Gin Val Val Leu 60 cct gat gac atc acc gtg gac act Pro Asp Asp Ilie Thr Val Asp Thr atc ccc ggc atc gcc cac gct ttt Ilie Pro Giy Ile Ala His Ala Phe ccg acc gca gta ttg tcg cgc acc Pro Thr Ala Val Leu Ser Arg Thr 105 ttc atc atg gcg ctg ccc ggc tcc Phe Ilie Met Ala Leu Pro Gly Ser 120 125 gct gtc ctc gac cca ctc att gat Ala Val Leu Asp Pro Leu Ile Asp 135 140 caccat gaa cac tgaccccgct tacgtf His His Gl'u His 150 <210> 75.0 <211> 153 <212> PRT <213> Corynebacterium glutamicum <400> 750 Vai Thr Ala Leu Val Ilie Vai Ala 1 Tyr Giu Asp Arg Ser Gly Pro Ile Gly Phe Asp Thr Pro Ala Pro Val Ala Phe Leu Asp Glu Leu Glu Phe 55 Gly Thr Gly Leu Thr Pro Asp Asp 70 Arg Leu Asp Lys Gu Ile Pro Gly Ser Met Asp Ala Val Pro Thr Ala 100 Thr Ile Gly Gly Ser Phe Ile Met 115 120 Ala Arg Asp Ala Thr Ala Val Leu att tca gi Ilie Ser G tta atc ci Leu Ile P.

tgg aat t~ rrp Asn 7I' 95 9tc gcg gi Val Ala G.

110 acg ggt gi rhr Gly A cac atc a His Ile T~ cgccg aac gc ly cg ac hr ggc Gly cgc Arg agc Ser acc Thr gcg Ala gga Gly 145 307 355 403 451 499 547 Ser Lieu IlieI Pro Ilie Ilie 2 Jtal 1 kla I ksp 1 1041 Arg Ser Ala Val1 Val1 His Ser Pro Leu Ala Trp Asp Val1 Asp Ala Arg Gly Ile WO 01/00843 WO 0100843PCT/EB00100923 135 Gly Thr Leu Gin Gly His His Glu His 145 150 <210> 751 <211> 1287 <212> DNA <213> Corynebacteriun glut amicun <220> <221> <222> <223>

CDS

(101)..(1264) R.XN0 0441 <400> 751 agccttggga gcggtgatta tgcttttggc tgtctatgtc ctcatcattg gagccatcgg agcgttacga ttgttttcca aggtgagaaa ggtttaattg atg tct cgt tcg ccg Met Ser Arg Ser Pro gag caa cat ttg gca gaa att tca gcg ctg ctt ccc cca caa Giu Gin His Leu Ala Giu Ile Ser Ala Leu Leu Pro Pro Gin aag tcc Lys Ser acg ttc gtg Thr Phe Val ctg cgc gaa gcg ttg gga cgc cgc acg Leu Arg Glu Ala Leu Gly Arg Arg Thr ttt tca gcg Phe Ser Ala gtc act gcg cag tgg gat tcg cca cgt ttt gat aat tcc caa atg gat Val Thr Ala Giln Trp Asp Ser Pro Arg Phe Asp Asn Ser Gin Met Asp ggc ttc Gly Phe gcg ctt ggc ccc Ala Leu Gly Pro t ca Ser cat ctt aac ggt His Leu Asn Gly acc ttc gca gtc Thr Phe Ala Val ggt Gly cca acc att ccc Pro Thr Ile Pro ggt cat gat cct Gly His Asp Pro cag tgg tac cca Gin Trp Tyr Pro ggc atc gaa aaa Gly Ile Glu Lys atc gcg ccg att atg acg ggt gcg cgc Ile Ala Pro Ilie Met Thr Gly Ala Arg ctt cct Leu Pro 100 aaa aac acc Lys Asn Thr ttc gac gcc Phe Asp Ala 120 gcc Ala 105 gcg atc att cct Ala Ile Ile Pro gtg Val 110 gag aaa acc aca Glu Lys Thr Thr ccg gga aat Pro Gly Asn 115 cca cag gta gaa Pro Gin Val Giu atc ccc gcc acc ccg caa ggt cag ttc Ile Pro Ala Thr Pro Gin Gly Gin Phe, 125 130 att act gcc ggc gac gag atc att cca Ile Thr Ala Gly Asp Glu Ile Ile Pro ata cgg Ile Arg 135 ttg cag ggt tcg Leu Gin Gly Ser gat Asp 140 gca ggt acg gag ctt aac tcg gtg cac atc ggg gtg ttg gct agt cag Ala Gly Thr Glu Leu'Asn Ser Val His Ile Gly Val Leu Ala Ser Gin 1042 WO 01/00843 150 tcg atc aa Ser Ile Ly acc ggc gg Thr Gly Gi gat gcc aa Asp Ala As gag gtc at Giu Val Ii 215 ttt gaa ct Phe Glu Le 230 acc tct gg Thr Ser Gi ctc gaa gg Leu Giu Gi ggc ggt cc Gly Gly Pr 28 ctt ccc gg 1027 Leu Pro Gi 295 gcg cca gc 1075 Ala Pro Al 310 acc gct cc 1123 Thr Ala Pr ggc acc at 1171 Gly Thr 11 ggg cac ca 1219 Gly His Gi 36 gat cag ga 1264 gca Ala gaa Glu cgt Arg 205 acc Thr gac Asp gg t Gly tgg Trp act Thr 285 ac c Thr ccg Pro c aa Gin ggg Gly ggt Gly 365 tac 160 c ca Pro gga Gly tgc Cys gat Asp caa Gin 240 gag Giu cat His gaa Giu agt Ser cac His 320 cgc Arg cct Pro tgc Cys gga ctc Leu acg Thr 195 aac Asn cga Arg gtc Val agg Arg cag Gin .275 gtc Val c tt Leu gcc Ala ttc Phe gcc Ala 355 aga Arg cat PCTLLBOO/00923 165 atc atc 643 Ile Ile 180 atc cct 691 Ile Pro aat atc 739 Asn Ile ctc cgc 787 Leu Arg atc atc 835 Ile Ile 245 cag atc 883 Gln Ile 260 cag cct 931 Gin Pro att tca 979 Ile Ser ttg gtc Leu Val cgc atc Arg Ile 325 ctt cgc Leu Arg 340 tct cct Ser Pro ctg tct Leu Ser cgt 1043 WO 01/00843 PCT/IB00/00923 Asp Gin Asp Pro Gly Ala Asp Tyr Gly Gly Gly Lys Arg His Arg 375 380 385 taagatttac ccattcaact aac 1287 <210> 752 <211> 388 <212> PRT <213> Corynebacterium glutamicum <400> 752 Met Ser Arg Ser Pro Glu Gln His Leu Ala Glu Ile Ser Ala Leu Leu 1 5 10 Pro Pro Gin Lys Ser Thr Phe Val Asn Leu Arg Glu Ala Leu Gly Arg 25 Arg Thr Phe Ser Ala Val Thr Ala Gin Trp Asp Ser Pro Arg Phe Asp 40 Asn Ser Gin Met Asp Gly Phe Ala Leu Gly Pro Ser His Leu Asn Gly 55 Gly Thr Phe Ala Val Gly Pro Thr Ile Pro Ala Gly His Asp Pro Asp 70 75 Gin Trp Tyr Pro Arg Gly Ile Glu Lys Asp Ile Ala Pro Ile Met Thr 90 Gly Ala Arg Leu Pro Lys Asn Thr Ala Ala Ile Ile pro Val Glu Lys 100 105 110 Thr Thr Pro Gly Asn Phe Asp Ala Pro Gin Val Glu Ile Pro Ala Thr 115 120 125 Pro Gin Gly Gin Phe Ile Arg Leu Gin Gly Ser Asp Ile Thr Ala Gly 130 135 140 Asp Glu Ile Ile Pro Ala Gly Thr Glu Leu Asn Ser Val His Ile Gly 145 150 155 160 Val Leu Ala Ser Gin Ser Ile Lys Ser Ile Glu Val Ala Ala Lys Pro 165 170 175 Arg Val Leu Ile Ile Thr Gly Gly Ser Glu Ile Ser Glu Gin His Gly 180 185 190 Pro Ala Thr Ile Pro Asp Ala Asn Gly Pro Leu Leu Arg Ser Leu Cys 195 200 205 Ala Arg Asn Asn Ile Glu Val Ile Ala Gly Leu His Thr Asn Asp Asp 210 215 220 Pro Glu Arg Leu Arg Phe Glu Leu Glu Asn Ala Ile Asp Gin Tyr Gin 225 230 235 240 Pro Asp Val Ile Ile Thr Ser Gly Gly Ile Ser His Gly Lys Phe Glu 245 250 255 1044 WO 01/00843 Val Phe Arg Gin Ile Leu Giu Gly Th 260 26 Val Asp Gin Gin Pro Gly Gly Pro Gi 275 280 Thr Pro Val Ile Ser Leu Pro Gly As 290 295 Phe Thr Leu Leu Val Ala Pro Ala Le 305 310 Leu Asp Ala Arg Ile Thr Ala Pro Va 325 Giu Gin Phe Leu Arg Gly Thr Ile Se 340 34 Arg His Ala Ser Pro Gly His Gin Ph 355 360 His Arg Arg Leu Ser Asp Gin Asp Pr 370 375 Lys Arg His Arg 385 <210> 753 <211> 815 <212> DNA <213> Corynebacterium giutatnicum <22 0> <221> CDS <222> (792) <223> FRXA00441 <400> 753 atc ccc gcc acc ccg caa ggt cag tt Ilie Pro Ala Thr Pro Gin Gly Gin Ph 1 5 att act gcc ggc gac gag atc att cc Ile Thr Ala Gly Asp Gu Ile Ile Pr 2 gtg cac atc ggg gtg ttg gct agt ca Val His Ilie Gly Val Leu Ala Ser Gi gca gca aag cca cgt gtc ctc atc at Ala Ala Lys Pro Arg Val Leu Ile Ii 55 gaa cag cac gga ccc gcc acg atc cc Giu Gin His Gly Pro Ala Thr Ilie Pr 70 cgt tcc ctg tgc gcc cgc aac aat at Arg Ser Leu Cys Ala Arg Asn Asn Ii Pro Gly Pro Asn Gin 330 Tyr Pro Gly ata Ile 10 gca Ala tcg Ser ac c Thr gat Asp gag Glu Asn Ile Ile Arg 315 Gly Arg Pro Ala cgg Arg ggt Gly atc Ile ggc Gly gcc Ala 75 gtC Val1 Ser Ser S er 300 Gin Leu Asn Ala Asp 380 ttg Leu acg Thr aag Lys ggg Gly aac Asn atc Ile Phe 270 Phe Leu Leu Asp Pro 350 Ser Gly ggt Gly ctt Leu att Ile gaa Giu cct Pro gga Gly PCTIIBOO/00923 Gly His Ala Giu Val Ser Arg His 320 Asn Arg 335 Arg Pro Ser Cys Gly Gly

C

a *0 5 g n

C

e t '0 c e tcg Ser aa c Asn gaa Giu att Ile c tg Leu c tg Leu gat Asp tcg Ser gtc Val tca Ser ctt Leu cac His 48 96 144 192 240 288 WO 01/00843 acc Thr gac Asp ggt Gly tgg Trp 145 act Thr ac c Thr ccg Pro c aa Gin ggg Gly 225 ggt Gly tac Tyr aac Asn cag Gin aaa Lys 130 ttt Phe ttt Phe ttg Leu ctc Leu gac Asp 210 cca Pro tca Ser ggt Gly gac Asp tat Tyr 115 ttt Phe gga Gly gct Ala gtg Val cgc Arg 195 aat Asn cgt Arg agc Ser gga Gly gat Asp 100 caa Gin gag Giu cat His gaa Glu agt Ser 180 cac His cgc Arg cct Pro tgc Cys gga Gly 260 cc t Pro ccg Pro gtg Val1 gtc Val act Thr 165 ttc Phe ctc Leu gag Glu cgc Arg c ac His 245 gaa Giu gat Asp ttt Phe ga t

ASP

150 cct Pro aca Thr gat Asp caa Gin cac His 230 cgc Arg cga Arg gtc Val agg Arg 135 cag Gin gtc Val ctt Leu gcc Ala t tc Phe 215 gcc Ala aga Arg ctc cgc ttt Leu Arg Phe 105 atc atc acc Ile Ile Thr 120 cag atc ctc Gin Ile Leu cag cct ggc Gin Pro Gly att tca ctt Ile Ser Leu 170 ttg gtc gcg Leu Val Ala 185 cgc atc acc Arg Ile Thr 200 ctt cgc ggc Leu Arg Gly tct cct ggg Ser Pro Gly ctg tct gat Leu Ser Asp 250 gaa Giu tct Ser gaa Giu ggt Gly 155 ccc Pro cca Pro gc t Ala acc Thr cac His 235 cag ctg Leu ggc Gly ggc Gly 140 cct Pro gga Gly gcg Ala c cg Pro atc Ile 220 cag Gin gat gaa Giu ggt Gly 125 acc Thr caa Gin aat Asn ctc Leu gtc Val1 205 agt Ser ttc Phe ccc PCT/1-B00100923 aac gcc att 336 Asn Ala Ile 110 atc agc cac. 384 Ile Ser His ccg aac tcc 432 Pro Asn Ser ggc atc tcc 480 Gly Ile Ser 160 ccg att tcc 528 Pro Ile Ser 175 aac cgc cag 576 Asn Arg Gin 190 cag ggc ttg 624 Gin Gly Leu tac cgc aac 672 Tyr Arg Asn cca cct gct 720 Pro Pro Ala 240 ggc gcg gac 768 Giy Ala Asp 255 Gin Asp Pro aaa cga cat cgt Lys Arg His Arg taagatttac ccattcaact aac <210> 754 <211> 264 <212> PRT <213> Corynebacterium giutamicum <400> 754 Ile Pro Ala Thr Pro Gin Gly Gin 1 5 Ile Thr Ala Val His Ile Asp Glu Ile Ile I Ser C 40 ?he Ile Arg Leu Gin Gly Ser Asp *10 'ro Ala Gly Thr Giu Leu Asn Ser fln Ser Ile Lys Ser Ile Giu Val Gly Val Leu Ala 1046 WO 01/00843 WO 0100843PCTIB0OO923 Ala Ala Lys Pro Arg Val Leu Ile Ile Thr Gly Gly Ser Giu Ile Ser 55 Giu Gin His Gly Pro Ala Thr Ile Pro Asp Ala Asn Gly Pro Leu Leu 70 75 Arg Ser Leu Cys Ala Arg Asn Asn Ile Giu Val Ile Ala Gly Leu His 90 Thr Asn Asp Asp Pro Giu Arg Leu Arg Phe Giu Leu Giu Asn Ala Ile 100 105 110 Asp Gin Tyr Gin Pro Asp Val Ile Ile Thr Ser Gly Gly Ile Ser His 115 120 .125 Gly Lys Phe Giu Val Phe Arg Gin Ile Leu Giu Gly Thr Pro Asn Ser 130 135 140 Trp Phe Gly His Val Asp Gin Gin Pro Gly Gly Pro Gin Gly Ile Ser 145 150 155 160 Thr Phe Ala Giu Thr Pro Val Ile Ser Leu Pro Gly Asn Pro Ile Ser 165 170 175 Thr Leu Val Ser Phe Thr Leu Leu Val Ala P ro Ala Leu Asn Arg Gin 180 185 190 Pro Leu Arg His Leu Asp Ala Arg Ilie Thr Ala Pro Val Gin Gly Leu 195 200 205 Gin Asp Asn Arg Giu Gin Phe Leu Arg Gly Thr Ile Ser Tyr Arg Asn 210 215 220 Gly Pro Arg Pro Arg His Ala Ser Pro Gly His Gin Phe Pro Pro Ala 225 230 235 240 Gly Ser Ser Cys His Arg Arg Leu Ser Asp Gin Asp Pro Gly Ala Asp 245 250 255 Tyr Gly Gly Gly Lys Arg His Arg 260 <210> 755 <211> 2358 <212> DNA <213> Corynebacteriun giutamicun <22 0> <221> CDS <222> (101)..(2335) <223> RXN02085 <400> 755 cacccggtga tttcgcgaac cttgaaacat cgtcagaaga ttgccgtgcg tcctagccgg gatccgcacg ttcggctcaa gcagaaagtc tttaactcac atg act tcc aac ttt 115 Met Thr Ser Asn Phe 1 1047 WO 01/00843 tct tcc ac Ser Ser Th aag ttc gc Lys Phe Al ctt gcg ca Leu Ala Gi 4 tct gga tt Ser Gly Le atg ctc ga Met Leu As gac atc gc Asp Ilie Al tac ttt gg Tyr Phe GI acc aag tg Thr Lys Tx 12 gcg gat ac Ala Asp TI! 135 cgt tgc ca Arg Cys GI 150 cca ctg ac Pro Leu T1, ttg gat ce Leu Asp Hi tct ttc ga Ser Phe AE 2C gat gtt gc Asp Val A] 215 ttg gct ae Leu Ala LN 230 ggt Gly ggc Gly cgc Arg gtt Val1 gc t Ala 75 gaa Glu cgc Arg acc Thr gtt Val cgt Arg 155 tcc Ser gca Ala tgg Trp gtt Val1 Gly 235 ctt Leu tac Tyr caa Gin ccg Pro att Ile aac Asn ggt Gly aac Asn t tg Leu 140 ggc Gly ctt Leu c tg Leu gtt Val ttg Leu 220 gtg Val cgt Arg gg t Gly ga t Asp tac Tyr cgt Arg att Ile cag Gin 115 gag Glu gag Giu ctg Leu tcC Ser ctc Leu 195 ttg Leu tac Tyr ggC Gly PCTIIBOO/00923 gaa ctg 163 Glu Leu cgc gaa 211 Arg Giu tct ttg 259 Ser Leu gac gca 307 Asp Ala ttt gat 355 Phe Asp gac cgc 403 Asp Arg 100 gca atg 451 Ala Met ttg tct 499 Leu Ser gat ctc 547 Asp Leu gtt ggt 595 Val Gly 165 aat cct 643 Asn Pro 180 atc aag 691 Ile Lys gtc acc 739 Val Thr acc act 787 Thr Thr tct ggc 835 Ser Gly 245 gat cag gcg ctg aac act ctt gcg ggc atc ggc ctt ggc gcg att ggc 883 1048 WO 01/00843 WO 0100843PCTIIBOO/00923 Asp Gin Ala Leu Asn 250 ac c Thr Thr Leu Ala Gly Ile 255 Gly Leu Gly Ala Ile Gly 260 gtt gac ttg Val Asp Leu gag gag ctg Glu Giu Leu 280 cat ggc gtc His Gly Val gag ctt gct Glu Leu Ala gat ggt cgt Asp Gly Arg gtt gcg ggc Val Ala Gly acc gac 1027 Thr Asp 295 ggc cca 1075 Gly Pro 310 acc ctc 1123 Thr Leu ttc ggc 1171 Phe Gly gcc ggc 1219 Ala Gly gct tct 1267 Ala Ser 375 cct ggc 1315 Pro Gly 390 aag tca 1363 Lys Ser cca cag 1411 Pro Gin tcc atc 1459 Ser Ile ctg tgt gct gct Leu Cys Ala Ala atc gca gtg tct Ile Ala Val Ser ctt Leu 300 tcc ctg aag cgc Ser Leu Lys Arg 305 gcg tgg aag ggt Ala Trp Lys Gly 275 aac att tgg cgc Asn Ile Trp Arg 290 ctg gca gct cgc Leu Ala Ala Arg cac gtt cct tac His Val Pro Tyr 325 gag gct gag Glu Ala Glu 330 tcg gag aag Ser Glu Lys acc tct tgt tca Thr Ser Cys Ser att gag cct gag le Giu Pro Glu 335 acc gag gtc aag Thr Giu Val Lys ctg Leu 320 c tg Leu atc Ile gtc cgc gac tgg ctt gcc Val Arg Asp Trp, Leu Ala 340 ctg ctt gcc gac gcc cta Leu Leu Ala Asp Ala Leu 355 gcg gcg tcc gca gca att Ala Ala Ser Ala Ala Ile aac Asn 345 350 atc gac gcg gct Ile Asp Ala Ala cgc acc tcc cca Arg Thr Ser Pro gcg Ala 365 t tC Phe gat 370 cgc acc gca cca atc acg cag gaa ctc Arg Thr Ala Pro Ile Thr Gin Giu Leu 385 ttc gac act cgt gtt acg ctg cag gag Phe Asp Thr Arg Val Thr Leu Gin Giu 400 405 cgt agc cgt gga Arg Ser Arg Gly 395 ctg gag ctt cca Leu Giu Leu Pro 410 gct ctg cca Ala Leu Pro acc cca Thr Pro 425 act ttg Thr Leu 440 tcc att cgt tct gct Ser Ile Arg Ser Ala 430 gag cag tac gaa gag Glu Gin Tyr Glu Giu 445 acc acc acc att ggt tct ttc Thr Thr Thr Ile Gly Ser Phe 415 420 cgc gct cgt ctg cgc aag gaa Arg Ala Arg Leu Arg Lys Giu 435 gca atg cgc gaa gaa atc gat Ala Met Arg Giu Glu Ile Asp 450 1049 WO 01/00843 WO 0100843PCTIBOO/00923 ctg gtc 1507 Leu Val 455 ggt gag 1555 Gly Giu 470 gac ggt 1603 Asp Gly cgc tgt 1651 Arg Cys cca atg 1699 Pro Met cat gtc 1747 His Val 535 ttc gtt 1795 Phe Val 550 ctg gca 1843 Leu Ala atc atc 1891 Ile Ile gac gtc 1939 Asp Val ctg- gcg 1987 Leu Ala 615 tgc tac 2035 Cys Tyr 630 atc gcc aag cag Ile Ala Lys Gin cca gag cgc aac Pro Glu Arg Asn 475 ttc ctc tca acc Phe Leu Ser Thr 490 gtt cgt cct cca Vai Arg Pro Pro gaa ctt ggt ctt gat gtg ttg gtt cac Giu Leu Gly Leu Asp Val Leu Vai His 465 atg gtt cag tac ttc tct gaa ctt ctc Met Vai Gin Tyr Phe Ser Glu Leu Leu gac Asp 480 gcc aac ggc tgg gtc Ala Asn Giy Trp Vai 495 caa agc tac ggc tcc Gin Ser Tyr Gly Ser 500 gtg ttg ttc Val Leu Phe 505 act gtc Thr Val 510 aag tgg ttc cag tac Lys Trp Phe Gin Tyr 525 atg ctc acc ggt cca Met Leu Thr Gly Pro gga aac gtt tcc cgc cca gcg Gly Asn Vai Ser Arg Pro Ala 515 gca cag agc ctg acc cag aag Ala Gin Ser Leu Thr Gin Lys 530 gtc acc atc ctt gca tgg tcc Vai Thr Ile Leu Ala Trp Ser gga Gly 540 545 cgc gat gat cag Arg Asp Asp Gin 555 ctg cgc gat gaa Leu Arg Asp Giu 570 ccg ctg gct acc Pro Leu Ala Thr att aac gat ctc Ile Asn Asp Leu act Thr 560 gc t Ala gac cag gtt gca Asp Gin Val Ala 565 cag gtg Gin Val 585 gat aag Asp Lys gat gag cct gcg att Asp Giu Pro Ala Ile 590 cct gcc tac ctg cag Pro Ala Tyr Leu Gin 605 ggc gca ccc gac gac Gly Ala Pro Asp Asp atc gag gct ggc gcg aag Ile Giu Ala Gly Ala Lys 580 gaa ctg ttg ccg cta cga Glu Leu Leu Pro Leu Arg 595 tcc gtg gac tcc ttc cgc Ser Val Asp Ser Phe Arg tgg Trp gcc Al a 610 gtc caa atc cac acc cac atg Val Gin Ile His Thr His Met tcc Ser 620 gag ttc aac gaa Glu Phe Asn Glu 625 gtg atc tcc tcg gtc Val Ile Ser Ser Val 640 atc gcg ttg gat Ile Ala Leu Asp 645 635 1050 WO 01/00843 gcc gat gtc 2083 Ala Asp Val gct gct ctg 2131 Ala Ala Leu tgg gat atc 2179 Trp Asp Ile 680 ctc ctc gag 2227 Leu Leu Giu 695 aac cca gac 2275 Asn Pro Asp 710 tcc cta aag 2323 Ser Leu Lys gga gca act 2358 Gly Ala Thr acc acc Thr Thr 650 aaa tct Lys Ser 665 cac tcc His Ser gct gca Ala Ala tgt ggt Cys Gly gtt ctc Val Leu 730 atc taa Ile 745 tcc gac Ser Asp ggc gtc Gly Val gcg cag Ala Gin cct cgc Pro Arg 705 tgg cca Trp Pro 720 cag gct Gin Ala PCTJIBOO/00923 gtc ctc Val Leu 660 ggt gtg Gly Val gac ggt Asp Gly tgg gtc Trp Val gaa gct Giu Ala 725 aaa atc Lys Ile 740 Val Giu Ser Ala attgggt taccgctagg aac <210> 756 <211> 745 <212> PRT <213> Corynebacteriun giutamicum <400> 756 Met Thr Ser Asn Phe Ser Ser Thr Val Ala 1 5 Ala Lys Arg Giu Leu Lys Phe Ala Leu Giu Ile Giu Gly Arg Giu Leu Ala Gin Thr Ala 40 Ala Ser Asp Ser Leu Ser Gly Leu Asp Ser 55 Ser Tyr Tyr Asp Ala Met Leu Asp Thr Ala Pro Giu Arg Phe Asp Asp Ile Ala Asp His Leu Trp Ilie Asp Arg Tyr Phe Gly Ala Ala 1051 Leu Tyr Gin Pro Ile Asn Gly Pro Trp Leu Phe Leu Asp Thr WO 01/00843 WO 0100843PCTIBOO/00923 Pro Ala Gin Val Leu 145 Pro Asp Glu Pro Ala 225 Tyr Leu Ala Arg Arg 305 Leu Arg Leu Ala Ile 385 Val1 Thr Pro 130 Ile Vai Gly Arg Ala 210 Gly Phe Gly Ala Asn 290 Leu His Asp Ala Ser 370 Thr Thr Ile 115 Glu Giu Leu Ser Leu 195 Leu Tyr Gly Ala Trp 275 Ilie Ala Val Trp Asp 355 Ala Gin Leu Gly 100 Ala Leu Asp Vai Asn 180 Ile Vai Thr S er Ile 260 Lys Trp, Ala Pro Leu 340 Ala Ala Glu Gin Ser 105 Phe Asp 110 His Tyr Leu Ser Leu Gly 165 Pro Lys Thr Thr Gly 245 Gly Gly Arg Arg Tyr 325 Ala Leu Ile Leu Glu 405 Phe Ala Arg 150 Pro Leu Ser Asp Leu 230 Asp Val Glu Thr Gly 310 Thr Phe Al1a Ala Pro 390 Lys Pro Met Thr Lys Trp Thr Asn Tyr Asp 135 Cys Leu Asp Phe Val 215 Ala Gin Asp Giu Asp 295 Pro Leu Gly Gly Se r 375 Gly Ser Gin 120 Thr Arg Gin Gin Thr Phe His Leu 185 Asp Thr 200 Ala Pro Lys Arg Ala Leu Leu Val 265 Leu Leu 280 Leu Cys Ile Ala Glu Ala Ser Glu 345 Asn Ile 360 Arg Arg Arg Ser Leu Giu Thr Pro 125 Phe Val Leu 170 Pro Giu Giu Asp Asn 250 Thr Val Al a Val Glu 330 Lys Asp Thr Arg Leu 410 Ser Val1 Arg 155 Ser Ala Trp, Val Gly 235 Thr His Ala Ala Ser 315 Asn Ile Ala Ser Gly 395 Pro Ile Leu 140 Gly Leu Leu Val1 Leu 220 Val1 Leu Gly Gly Leu 300 Thr Ile Thr Ala Pro 380 Ser Ala Arg Asp Val Al a Phe Gin 205 Glu Phe Ala Val Ile 285 Ala Ser Glu Glu Al a 365 Arg Phe Leu Ser Ala Asn Arg Glu 190 Ile Gin Val Gly Thr 270 Val Ser Cys Pro Val1 350 Phe Thr Asp Pro Ala 430 Ser Ala Ala Arg 160 Thr Thr 175 Val Tyr Asp Glu Val Arg Asn Thr 240 Ile Gly 255 Glu Leu Asp Gly Leu Lys Ser Leu 320 Glu Val 335 Lys Leu Asp Ala Ala Pro Thr Arg 400 Thr Thr 415 Arg Ala 1052 WO 01/00843 WO 0100843PCT/IBO0100923 Arg Arg Asp 465 Phe Gin Val Ser Ile 545 Ala Glu Leu Val1 Ile 625 Val Asp Val1 Gin Arg 705 Pro Al a Lys Ile Val Leu Gly 500 Pro Gin Trp Val Ala 580 Leu Phe His Leu Val 660 Gly Asp Trp Giu Lys 740 Giu Asp His Leu 485 Ser Ala Lys Ser Ala 565 Lys Arg Arg Met Asp 645 Leu Val Gly Val1 Al a 725 Ile Ser Leu Gly 470 Asp Arg Pro His Phe 550 Leu Ile Asp Leu Cys 630 Al a Ala Trp Leu Asn 710 Ser Gly Ile Val 455 Glu Gly Cys Met Val 535 Val1 Ala Ile Val Ala 615 Tyr Asp Ala Asp Leu 695 Pro Leu Ala Glu 460 Asp Ala Val1 Phe Thr 540 Pro Ile Pro Tyr Pro 620 Giu Giu Gly Arg Gin 700 Lys Glu Giu 445 Giu Met Asn Leu Gin 525 Gly Leu Asn Ala Leu 605 Asp Vai Ala Phe Val1 685 Ser Thr Ser Glu Leu Val Giy Phe 510 Tyr Pro Ala Asp Ile 590 Gin Asp Ile Ala Glu 670 Pro Vai Arg Ala Aia Gly Gin Trp 495 Giy Al a Vai Thr Leu 575 Arg Trp Vai Ser Arg 655 Leu Ser Asp Gly Lys 735 Met Leu Tyr 480 Val1 Asn Gin Thr Thr 560 Ile Glu S er Gin Ser 640 Ser Gly Ala Pro Trp 720 Gin 1053 WO 01/00843 WO 0100843PCT/IBOO/00923 <210> 757 <211> 1923 <212> DNA <213> Corynebacterium glutamicum <220> <221> <222> <223>

CDS

(101)..(1900) FRXA02 085 <400> 757 cacccggtga tttcgcgaac cttgaaacat cgtcagaaga ttgccgtgcg tcctagccgg gatccgcacg ttcggctcaa gcagaaagtc tttaactcac atg act tcc aac Met Thr Ser Asn 115 163 ict tcc act gtc gct ggt ctt cct cgc Ser Ser Thr Val Ala Gly Leu Pro Arg gga gcg aag cgt Gly Ala Lys Arg gaa ctg Giu Leu aag ttc gcg Lys Phe Ala ctt gcg cag Leu Ala Gin gaa ggc tac tgg Giu Gly Tyr Trp gga tca att gaa Gly Ser Ile Glu ggt cgc gaa Gly Arg Giu gat tct ttg Asp Ser Leu acc gcc cgc caa Thr Ala Arg Gin gtc aac act gca Val Asn Thr Ala tct gga Ser Gly ttg gat tcc gtt Leu Asp Ser Val ttt gca gga cgt Phe Ala Gly Arg tcc Ser tac tac gac gca Tyr Tyr Asp Ala ctc gat acc gcc gct att ttg ggt gtg Leu Asp Thr Ala Ala Ile Leu Gly Val ccg gag cgt ttt Pro Glu Arg Phe gac atc gct gat Asp Ile Ala Asp gaa aac gat ggt Glu Asn Asp Gly cca ctg tgg att Pro Leu Trp Ile gac cgc Asp Arg 100 tac ttt ggc Tyr Phe Gly acc aag tgg Thr Lys Ti-p 120 gct cgc ggt act Ala Arg Gly Thr acc ctg cct gca cag gca atg Thr Leu Pro Ala Gin Ala Met 115 ttt gat acc aac Phe Asp Thr Asn cac tac ctc gtg His Tyr Leu Val cog Pro 130 gag ttg tct Giu Leu Ser gcg gat Ala Asp 135 aca cgt ttc gtt Thr Arg Phe Val ttg Leu 140 gat gcg tcc gcg Asp Ala Ser Ala att gag gat otc Ile Giu Asp Leu tgc cag cag gtt Cys Gin Gin Val cgt Arg 155 ggc gtt aat gcc Gly Val Asn Ala Oct gtt ctg gtt Pro Val Leu Val cca ctg act ttc Pro Leu Thr Phe ctt Leu 170 too ctt gct cgc Ser Leu Ala Arg act gat ggt too Thr Asp Gly Ser aat Oct Asn Pro 180 1054 WO 01/00843 WO 0100843PCT/EB00100923 ttg gat Leu Asp tct ttc Ser Phe gat gtt Asp Val 215 ttg gct Leu Ala 230 gat cag Asp Gin gtt gac Val Asp gag gag Giu Glu acc gac 1027 Thr Asp 295 ggc cca 1075 Gly Pro 310 acc ctc 1123 Thr Leu ttc ggc 1171 Phe Gly gcc ggc 1219 Ala Gly gct tct 1267 Ala Ser 375 cct ggc 1315 cc t Pro gag Glu gag Giu gat Asp aac Asn 250 acc Thr gtt Val gct Ala gtg Val1 gag Giu 330 aag Lys gac Asp ac c Thr cgt ctg ttt Leu Phe gtt cag Val Gin 205 ttg gag Leu Glu 220 gtg ttt Val Phe ctt gcg Leu Ala ggc gtc Gly Val ggc atc Gly Ile 285 ctt gct Leu Ala 300 acc tct Thr Ser att gag Ile Glu acc gag Thr Giu gct gcg Ala Ala 365 cca cgc Pro Arg 380 tcc ttc gag Giu cc t Pro gct Ala 225 tac Tyr ctt Leu gct Ala cgt Arg cgc Arg 305 ctg Leu cgc Arg ctt Leu gcg Ala atc Ile 385 gtt cgc Arg gcg Al a 210 gg t Gly ttc Phe ggc Gly gcg Al a aac Asn 290 ctg Leu cac His gac Asp gcc Ala t cc Ser 370 acg Thr acg 1055 WO 01/00843 WO 0100843PCTIIBOOIOO923 Pro Gly 390 aag tca 1363 Lys Ser cca cag 1411 Pro Gin tcc atc 1459 Ser Ile ctg gtc 1507 Leu Val 455 ggt gag 1555 Gly Giu 470 gac ggt 1603 Asp Giy cgc tgt 1651 Arg Cys cca atg 1699 Pro Met cat gtc 1747 His Val 535 ttc gtt 1795 Phe Val 550 ctg gca 1843 Leu Ala atc atc 1891 Ile Ile Arg Ser Arg ctg gag ctt Leu Giu Leu 410 Ser Phe Asp Thr gct ctg cca acc Ala Leu Pro Thr 415 Arg 400 acc Thr Val Thr Leu Gin acc att ggt tct ttc Thr Ile Gly Ser Phe 420 acc cca Thr Pro 425 act ttg Thr Leu tcc att cgt tct gct Ser Ile Arg Ser Ala 430 gag cag tac gaa gag Giu Gin Tyr Giu Giu 445 aag cag gaa gaa ctt Lys Gin Giu Glu Leu cgc gct cgt ctg cgc aag gaa Arg Ala Arg Leu Arg Lys Giu 435 gca atg cgc gaa gaa atc gat Ala Met Arg Glu Giu Ile Asp 450 ggt ctt gat gtg ttg gtt cac Giy Leu Asp Val Leu Val His 440 atc Ile gcc Ala 460 465 cca gag cgc aac Pro Giu Arg Asn 475 ttc ctc tca acc Phe Leu Ser Thr 490 gac atg gtt cag tac Asp Met Vai Gin Tyr 480 gcc aac ggc tgg gtc Aia Asn Gly Trp Val 495 ttc tct gaa ctt ctc Phe Ser Giu Leu Leu 485 caa agc tac ggc tcc Gin Ser Tyr Gly Ser 500 gtt cgt Vai Arg 505 act gtc Thr Val cct cca Pro Pro gtg ttg ttc Val Leu Phe 520 510 aag tgg ttc cag tac Lys Trp Phe Gin Tyr 525 atg ctc acc ggt cca met Leu Thr Gly Pro gga aac gtt tcc cgc cca gcg Gly Asn Val Ser Arg Pro Ala 515 gca cag agc ctg acc cag aag Ala Gin Ser Leu Thr Gin Lys 530 gtc acc atc ctt gca tgg tcc Val Thr Ile Leu Ala Trp Ser aag gga Lys Gly 540 545 cgc gat gat cag Arg Asp Asp Gin 555 ctg cgc gat gaa Leu Arg Asp Giu 570 cag gtg gat gag Gin Val Asp Giu ccg Pro ctg gct acc act Leu Ala Thr Thr 560 gct Ala gac cag gtt gca Asp Gin Val Ala 565 att aac gat ctc Ile Asn Asp Leu 575 cct gcg att cgt Pro Ala Ile Arg atc gag-gct ggc gcg aag Ile Giu Ala Gly Ala Lys 580.

gaa ctg ttg ccc gct acg Giu Leu Leu Pro Ala Thr 1056 WO 01/00843 WO 0100843PCT/EBOO/00923 585 590 595 aga cgt cga taagcctgcc tacctgcagt ggt 1923 Arg Arg Arg 600 <210> 758 <211> 600 <212> PRT <213> Corynebacterium giutainicum <400> 758 Met Thr Ser ASn Phe Ser Ser Thr Val Ala Gly Leu Pro Arg Ile Gly 1 5 10 Ala Lys Arg Giu Leu Lys Phe Ala Leu Glu Gly Tyr Ti-p Asn Gly Ser 25 Ile Glu Gly Arg Glu Leu Ala Gin Thr Ala Arg Gin Leu Val Asn Thr 40 Ala Ser Asp Ser Leu Ser Gly Leu Asp Ser Val Pro Phe Ala Gly Arg 55 Ser Tyr Tyr Asp Ala Met Leu Asp Thr Ala Ala Ile Leu Gly Val Leu 70 75 Pro Glu Arg Phe Asp Asp Ile Ala Asp His Glu Asn Asp Gly Leu Pro 90 Leu Trp Ile Asp Arg Tyr Phe Gly. Ala Ala Arg Gly Thi- Giu Thr Leu 100 105 110 Pro Ala Gin Ala Met Thr Lys Ti-p Phe Asp Tiri Asn Tyr His Tyr Leu 115 120 125 Val Pro Giu Leu Ser Ala Asp Thr Arg Phe Val Leu Asp Ala Ser Ala 130 135 140 Leu Ile Giu Asp Leu Arg Cys Gin Gin Val Arg Gly Val Asn Ala Arg 145 150 155 160 Pro Val Leu Val Gly Pro Leu Thi- Phe Leu Ser Leu Ala Arg Thi- Thi- 165 170 175 Asp Gly Ser Asn Pro Leu Asp His Leu Pro Ala Leu Phe Giu Val Tyr 180 185 190 Giu Arg Leu Ile Lys Ser Phe Asp Thr Giu Ti-p Val Gin Ile Asp Giu 195 200 205 Pro Ala Leu Val Thi- Asp Val Ala Pro Giu Val Leu Giu Gin Val Arg 210 215 220 Ala Gly Tyr Thi- Thi- Leu Ala Lys Arg Asp Gly Val Phe Val Asn Thr 225 230 235 240 Tyr Phe Gly Ser Gly Asp Gin Ala Leu Asn Thr Leu Ala Gly Ile Gly 245 250 255 1057 WO 01/00843 WO 0100843PCTILBOO/00923 Leu Ala Arg Arg 305 Leu Arg Leu Ala Ile Val Thr Arg Arg.

Asp 465 Phe Gin Val Ser Ile 545 Ala Gly Al a Asn 290 Leu His Asp Ala Ser 370 Thr Thr Ile Leu Glu 450 Val Ser Ser Ser Leu 530 Leu Asp Ala Trp 275 Ile Ala Val1 Trp Asp 355 Ala Gin Leu Gly Arg 435 Giu Leu Glu Tyr Arg 515 Thr Ala Gin Ile 260 Lys Trp Ala Pro Leu 340 Ala Ala Glu Gin Ser 420 Lys Ile Val1 Leu Gly 500 Pro Gin Trp Val Gly Gly Arg Arg Tyr 325 Ala Leu Ile Leu Giu 405 Phe Giu Asp His Leu 485 Ser Ala Lys Ser Ala 565 Leu Leu 280 Leu Ile Glu Ser Asn 360 Arg Arg Leu Thr Thr 440 Ile Pro Phe Val Thr 520 Lys Arg Leu Thr Val Ala Val Giu 330 Lys Asp Thr Arg Leu 410 Ser Gi u Lys Arg Ser 490 Pro Lys Met Asp Asp 570 His Ala Ala Ser 315 Asn Ile Ala Ser Gly 395 Pro Ile Gin Gin Asn 475 Thr Pro Trp Leu Gin 555 Glu Gly Gly Leu 300 Thr Ile Thr Ala Pro 380 Ser Ala Arg Tyr Giu 460 Asp Ala Val Phe Thr 540 Pro Ile Val Ile 285 Ala Ser Glu Glu Al a 365 Arg Phe Leu Ser Glu 445 Giu Met Asn Leu Gin 525 Gly Leu Asn Thr 270 Val Ser Cys Pro Val 350 Phe Thr Asp Pro Ala 430 Glu Leu Val Gly Phe 510 Tyr Pro Ala Asp Glu Asp Leu Ser Giu 335 Lys Asp Ala Thr Thr 415 Arg Ala Gly Gin Trp 495 Gly Ala Val Thr Leu 575 1058 WO 01/00843 WO 0100843PCT/EBOO/00923 Glu Ala Gly Ala Lys Ile Ile Gin Val Asp Giu Pro Ala Ile Arg Giu 580 585 590 Leu Leu Pro Ala Thr Arg Arg Arg 595 600 <210> <211> <212> <213> <220> <221> <222> <223> 759 603

DNA

Corynebactzerium giutainicum

CDS

(101)..(580) FRXA0 2086 <400> 759 gatgatcagc cgctggctac cactgctgac caggttgcac tggcactgcg cgatgaaatt aacgatctea tcgaggctgg cgcgaagate atccaggtgg atg age ctg cga Met Ser Leu Arg 1 gtg aac tgt tgc Val Asn Cys Cys ecg Pro eta cga gac gtc Leu Arg Asp Val aag cet gcc tac Lys Pro Ala Tyr ctg eag Leu Gin tgg tee gtg Trp Ser Val gtc caa atc Val Gin Ile tce ttc cgC ctg Ser Phe Arg Leu act gcc ggc gca.

Thr Ala Gly Ala ccc gac gac Pro Asp Asp gaa gtg atc Giu Val Ile cac acc cae atg His Thr His Met tac tec gag tte Tyr Ser Giu Phe aac Asn tcc tcg Ser Ser gtc atc gcg ttg Val Ile Ala Leu gcc gat gtc ace Ala Asp Val Thr atc gaa gca gca Ile Giu Ala Ala cgt Arg tee gac atg cag Ser Asp Met Gin gtc Val etc gct get ctg Leu Ala Ala Leu tet tee ggc tte Ser Ser Gly Phe cte ggc gtc gga Leu Gly Val Gly ggt gtg tgg gat Gly Val Trp Asp cac tee ceg ege His Ser Pro Arg gtt ect Val Pro 100 tee geg cag Ser Ala Gin gat cet ege Asp Pro Arg 120 gtg gae ggt etc etc gag get gca etg Val Asp Gly Leu Leu Giu Ala Ala Leu 110 eag tee gtg Gin Ser Val 115 aag ace egt Lys Thr Arg cag etg tgg gte Gin Leu Trp Val eca gae tgt ggt Pro Asp Cys Gly gga tgg Gly Trp 135 eca gaa gtg gaa Pro Giu Val Giu get Ala 140 tee eta aag gtt Ser Leu Lys Val etc Leu 145 gtt gag tee get Val Giu Ser Ala aag eag get egt gag aaa ate gga gca act ate taaattgggt tacegctagg 600 1059 WO 01/00843 Lys Gin Ala Arg Glu Lys Ile Gly Ala Thr Ile 150 155 160 aac <210> 760 <211> 160 <212> PRT <213> CorynebacteriumT giutaxnicum PCTJIBOO/00923 603 <400> 760 Met Ser Leu Arg Phe Val Asn Cys Cys 1 Pro Giy Phe Thr Ser Ser Ala Gly Leu 145 5 Gin Asp Ile Ala Giu Pro Val1 Arg Ala Trp Val Ser Arg 70 Leu Ser Asp Giy Lys 150 Val1 Ilie Val1 Asp Vai Gin Arg 120 Pro Ala Leu Phe Hius Leu Vai 75 Gly Asp Trp, Giu Lys 155 Asp Leu Cys Ala Ala Trp Leu Asn 125 Ser Gly Val Ala Tyr Asp Al a Asp Leu 110 Pro Leu Ala Asp Thr Ser Val Leu Ile Giu Asp Lys Thr Lys Ala Glu Thr Lys His Ala Cys Val Ile 160 <210> 761 <211> 1326 <212> DNA <213> Corynebacterium giutamicun <22 0> <221> CDS <222> (101)..(1303) <223> RXN02648 <400> 761 atgaataaaa ttccgggtgc agtgaccgta ggtgaggtaa acgcggttag agtcgaatga gagtttgata ctttctttcg acttttagat tggattttca atg agc cag aac cgc 115 Met Ser Gin Asn Arg 1060 WO 01/00843 atc Ile gat Asp ttc Phe gac Asp tcc Ser c tg Leu gca Ala cgt Arg ggc Gly cc t Pro 150 c tg Leu gca Ala act Thr tac Tyr gac

ASP

230 acc Thr aac Asn att Ile ggt Gly gca Al a gga Gly cgt Arg gac Asp 120 ttc Phe acc Thr aag Lys tcc Ser gaa Glu 200 atc Ile gca Ala act Thr atc Ile ctg Leu atc Ile gtt Val ctg Leu tcc Ser 105 cgc Arg acc Thr tac Tyr ggc Gly cca Pro 185 gaa Glu atc Ile gaa Glu cac His aag Lys cag Gin gac

ASP

gac Asp acc Thr acc Thr gca Ala ggt Gly at t Ile a tg Met 170 gga Gly gtc Val1 acc Thr gca Ala gtt Val cgt Arg tct Ser atc Ile t tc Phe 75 atg Met cct Pro ttg Leu cgc Arg ggc Gly 155 aac Asn tc t Ser gtc Val gat Asp tgg Trp 235 1 ttg ccc cgt acc Leu Pro Arg Thr 15 ggt gag att ggg Gly Glu Ile Gly gat gac gtg atc Asp Asp Val Ile gag ggc gaa tac Glu Gly Giu Tyr tgg tgg aac tac Trp Trp, Asn Tyr 80 acc gac cgt tgg Thr Asp Arg Trp atc gag ctg acc Ile Glu Leu Thr 110 gaa gca tac gag Glu Ala Tyr Giu gtg ggc aac cca Val Gly Asn Pro 145 gaa act cag acg Giu Thr Gin Thr 160 gga gct acc gac Gly Aia Thr Asp 175 cga ttg acc aac Arg Leu Thr Asn 190 tgt gct gat gcg Cys Ala Asp Ala ctg acc gtt cag Leu Thr Vai Gin 225 atc aac cca gag Ile Asn Pro Giu 240 cc a Pro gag Giu aag Lys ggc Gly tcc Ser gca Ala agc Ser gat Asp 130 gag Giu gat Asp ggc Gly aag Lys ctt Leu 210 ctc Leu PCTIIBOO/00923 a ctt 163 au Leu 3a ttc 211 lu Phe gig gtt 259 1n Val c acc 307 al Thr cgc 355 qr Arg ag gaa 403 1n Giu :t gat 451 ar Asp t a tct 499 31 Ser c gga 547 .ir Gly It ctg 595 sp Leu 165 :t gca 643 31 Ala 5ic gac 691 Orr Asp 3g gaa 739 Ln Giu :a ccg 787 La Pro cca agc gtg aag Pro Ser Val Lys WO 01/00843 WO 0100843PCT/EBOO/00923 gat tac ttg gac tgg Asp Tyr Leu Asp Trp 250 cca Pro atc ggt Ile Gly aag gaa Lys Giu aca cgc atc gat gcc atc aac agt gca Thr Arg Ile Asp Ala Ile Asn Ser Ala 255 260 cag acc cgc ctg cac atc tgc tgg ggc Gin Thr Arg Leu His Ile Cys Trp Gly gtg aag ggc Val Lys Giy tct tgg cac Ser Trp His 280 270 gac Asp cca cac gtc Pro His Val act Thr 285 gtc Val1 atc cca ttc Ile Pro Phe ggt gac atc att 979 Gly Asp Ilie Ile 290 ttc gaa ggc gca Phe Giu Giy Ala ggt gag 1027 Gly Giu 295 tct cct 1075 Ser Pro 310 cct gaa 1123 Pro Giu gct gtg 1171 Ala Vai aag ctt 1219 Lys Leu atc ctg cgc gca gag Ilie Leu Arg Ala Giu 300 cgt cac gca cac gag Arg His Ala His Giu 315 ggc tct gtt atc tac Giy Ser Val Ile Tyr 330 gag cac cca cgc ctg Glu His Pro Ara Leu ggt ggc ttc tcc Gly Gly Phe Ser .305 tgg cgt gta tgg Trp Arg Val Trp, 320 cct ggt gtt gtg Pro Gly Val Val 335 gtt gct gat cgt Val Ala Asp Arg gaa gaa aac aag ctt Giu Giu Asn Lys Leu 325 tct cac tcc atc aac Ser His Ser Ile Asn 340 345 gtt ggc Val Gly cct gag aac gtc Pro Giu Asn Val 365 cat tcc cag atc His Ser Gin Ilie 350 att gcg tcc Ilie Ala Ser atc gtt cag ttc gcc Ile Val Gin Phe Ala 355 act gac tgt ggt ctg Thr Asp Cys Giy Leu ggc gga cgi ctE 1267 Gly Gly Arg Le~ 375 gta gag ggc gcl 1313 Val Giu Giy Al 390 aacgagggtt gct 1326 .1 370 gca tgg gca aag ctg Ala Trp Ala Lys Leu gag tcc cta Giu Ser Leu 380 t cgc att gca a Arg Ile Ala 395 tca aag gaa ctg Ser Lys Giu Leu 400 taagctagac <210> 762 <211> 401 <212> PRT <213> Corynebacterium giutamicui <400> 762 Met Ser Gin Asn Arg Ilie Arg Thr Thr 1 5 His Val Giy Ser Leu Pro Arg 10 1062 WO 01/00843 Thr Pro Giu Gly Glu Giu Ilie Lys Arg Tyr Gly His Tyr Ser Phe Trp Ala Ser Thr Ser Phe 115 Glu Asp Pro 130 Pro Glu Phe 145 Thr Asp Val Asp Gly Phe Asn Lys Phe 195 Ala Leu Ser 210 Gin Leu Asp 225 Giu Pro Ser Ala Ilie Asn His Ilie Cys 275 Phe Gly Asp 290 Ser Phe Giu 305 Giu Glu Asn Leu Giu Gin Val Thr Gin 100 Ser Val Thr Asp Val 180 Gin Ala Val Ser 260 Trp Ile G ly Lys Leu Phe Val Thr Arg Giu Asp Ser Gly Leu 165 Ala Asp Giu Pro Lys 245 Ala Gly Ile Ala Leu 325 Asp Phe Asp Ser 70 Leu Ala Arg Gly Pro 150 Leu Ala Thr Tyr Asp 230 Asp Val1 Ser Gly Ser 310 Pro Ile 25 Leu Ile Val1 Leu Ser 105 Arg Thr Tyr Gly Pro 185 Glu Ile Giu Asp Leu 265 Gly Leu His Ser Lys Gin Asp Asp Thr 90 Thr Ala Gly Ile Met 170 Gly Val Thr Ala Trp 250 Pro Pro Arg Ala Val1 330 Arg Ser Ile Phe 75 Met Pro Leu Arg Gly 155 Asn Ser Val Asp Trp 235 Ile Lys His Ala His 315 Ile Ser Ser Leu Giy Thr Gly Phe Ala 140 Gin Al a Ala Ala Al a 220 Asp Gly Giu Val1 Giu 300 Glu Tyr Asn Val1 Asn Ala Asp Asn Ser 125 Ser Glu Ala Ala Ala 205 Gly Gin Thr Gin Thr 285 Val Trp Pro Giy Asp Giu Trp Thr Ilie 110 Glu Val Glu Gly Arg 190 Cys Leu Ile Arg Trhr 270 Asp Gly Arg Gly PCTIIBOO/00923 Glu Ile Asp Val Giy Glu Trp Asn Asp Arg Giu Leu Ala Tyr Gly Asn Thr Gin 160 Ala Thr 175 Leu Thr Ala Asp Thr Val Asn Pro 240 Ile Asp 255 Arg Leu Ile Pro Gly Phe Val Trp 320 Val Val 335 Ser His Ser Ile Asn Ala Val Glu His Pro Arg Leu Val Ala Asp Arg 1063 WO 01/00843 WO 0100843PCT/EBOO/00923 340 345 350 Ile Val Gin Phe Ala Lys Leu Val Giy Pro Giu Asn Val Ile Ala Ser 355 360 365 Thr Asp Cys Gly Leu Gly Gly Arg Leu His Ser Gin Ile Ala Trp Ala 370 375 380 Lys Leu Giu Ser Leu Val Giu Gly Ala Arg Ile Ala Ser Lys Giu Leu 385 390 395 400 Phe <210> 763 <211> 548 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (525) <223> FRXA02648 <400> 763 gac gca ccg gac Asp Ala Pro Asp 1 agc gtg aag gat Ser Val Lys Asp aac agt gca gtg Asn Ser Ala Val tgc tgg ggc tct Cys Trp Gly Ser gac atc att ggt Asp Ile Ile Gly gaa ggc gca tct Giu Gly Ala Ser aac aag ctt cct Asn Lys Leu Pro 100 tcc atc aac gct Ser Ile Asn Ala 115 gaa gca tgg gat Giu Ala Trp Asp 10 gac tgg atc ggt Asp Trp Ilie Gly ctt cca aag gaa Leu Pro Lys Giu gga cca cac gtc Gly Pro His Val 55 ctg cgc gca gag Leu Arg Ala Giu cac gca cac gag His Ala His Giu tct gtt atc tac Ser Val Ile Tyr 105 cac cca cgc ctg His Pro Arg Leu 120 ggc cct gag aac Gly Pro Giu Asn 135 cag Gin aca Thr cag Gin act Thr gtc Val 75 tgg Trp cct Pro gtt Val gtc Val atc Ile cgc Arg acc Thr gac Asp ggt Gly cgt Arg ggt Gly gc t Ala att Ile 140 gag Giu gcc Ala cac Hius ttc Phe tcc S er gaa Giu tct Ser atc Ile act Thr c ca Pro atc Ile atc Ile ggt Gly t tc Phe gaa Giu cac His gtt Val gac

ASP

cag ttc gcc aag ctt gtt Gin Phe Ala Lys Leu Val 130 1064 WO 01/00843 WO 0100843PCT/iBOOI00923 tgt ggt ctg ggc gga cgt ctg cat tcc cag atc gca tgg gca aag ctg Cys Gly Leu Gly Gly Arg Leu His Ser Gin Ile Ala Trp Ala Lys Leu 145 150 155 160 gag tcc cta gta gag ggc gct cgc att gca tca aag gaa ctg ttc Giu Ser Leu Val Giu Gly Ala Arg Ile Ala Ser Lys Glu Leu Phe 165 170 175 taagctagac aacgagggtt gct <210> 764 <211> 175 <212> PRT <213> Corynebacterium giutaxnicum <400> 764 Asp Ala Pro Asp Leu Ala Giu Ala Trp Asp Val1 Ser Gly Ser Pro 100 Ala Lys Gly Tyr Lys Trp Giu Pro Giu Val Leu Gly Leu Gly His Ile Arg Gly Giu Val1 Arg 150 Asp Leu Gly Leu His Ser His Gly 135 Leu Trp, Pro Pro Arg Ala Val Pro 120 Pro His Ile Lys His Ala His Ile 105 Arg Glu Ser Asp 10 Gly Glu Val Glu Giu 90 Tyr Leu Asn Gin Ala 170 Gin Thr Gin Thr Val 75 Trp Pro Val Val1 Ile 155 Ile Arg Thr Asp Gly Arg Gly Ala Ile 140 Ala Asn Ile Arg Ile Gly Val Val Asp 125 Ala Trp, Giu Ala His Phe Ser Gi u Ser Ile Thr Lys Pro Ile Ile Gly Phe Giu His Val Asp Leu 160 Val Giu 165 Gly Ala Arg Ile Ser Lys Giu Leu <210> 765 <211> 784 <212> DNA <213> Corynebacterium giutamicum <22 0> <221> CDS <222> (101)..(784) <223> FRXA02658 1065 WO 01/00843 WO 0100843PCTA~BOO/00923 <400> 765 atgaataaaa ttccgggtgc agtgaccgta ggtgaggtaa acgcggttag agtcgaatga gagtttgata ctttctttcg acttttagat tggattttca atg agc cag aac cgc Met Ser Gin Asn atc Ile gat Asp ttc Phe gac Asp tcc Ser ctg Leu gca Al a cgt Arg ggC Gly cc t Pro 150 c tg tLeu gca Ala act Thr ca c His aag Lys cag Gin gac Asp gac Asp acc Thr acc Thr gca Ala ggt Gly att Ile atg Met 170 gga Gly gtc Val gtt Vai cgt Arg tct Ser atc Ile ttC Phe 75 a tg Met cc t Pro ttg Leu cgc Arg ggC Gly 155 aac Asn tc t Ser gtc Val ggt Gly tct Ser tct Ser ctt Leu ggt Gly acc Thr ggc Gly ttc Phe gct Ala 140 cag Gin gca Ala gca Ala gca Ala tcc Ser aac Asn gta Val1 aac Asn gca Ala gat Asp aac Asn agc Ser 125 tc t Ser gaa Giu gcg Ala gc t Ala gca Ala 205 ttg Leu ggt Gly gat Asp gag Giu tgg Trp ac C Thr atc Ile 110 gaa Giu gtg Val1 gaa Giu gga Gly cga Arg 190 tgt Cys

CCC

Pro 15 gag Giu gac Asp ggc Gly tgg Trp gac Asp 95 gag Giu gca Ala ggc Gly act Thr gct Ala 175 t tg Leu gc t Ala 1 acc Thr ggg Gly atc Ile t ac Tyr tac Tyr tgg Trp acc Thr gag Giu c ca Pro 145 acg Thr gac Asp aac Asn gcg Ala cca Pro gag Giu aag Lys ggc Gly tcc Ser gca Ala agc Ser gat Asp 130 gag Glu gat Asp ggc Gly aag Lys ct t Leu 210 gag Glu gag Glu cgc Arg cac His ttc Phe agc Ser ttc Phe 115 cca Pro t tc Phe gtt Val t tc Phe ttc Phe 195 tcc Ser c ta Leu gaa Giu cag Gin gtc Val acc Thr cag Gin 100 tc t Ser gta Val acc Thr ga t Asp gtt Val1 180 tac Tyr cag Gin Arg ctt Leu t tc Phe gtt Val acc Thr cgc Arg gaa Glu gat Asp tct Ser gga Gly Ctg Leu 165 gca Al a gac Asp gaa Glu 115 163 211 259 307 355 403 451 499 547 595 643 691 739 tac aag atc atc acc gat gca ggt ctg acc gtt cag ctc gac gca 1066 WO 01/00843 PCTIIBOO/00923 Tyr Lys Ile Ile Thr Asp Ala Gly Leu Thr Val Gin Leu Asp Ala 215 220 225 <210> 766 <211> 228 <212> PRT <213> Corynebacterium giutainicum <400> 766 Met Ser Gin Asn Arg Ilie Arg Thr Thr 1 Thr Pro G: Gly Giu G.' Ile Lys A: Tyr Gly H: Tyr Ser P1 Trp Ala Si Thr Ser P1 1: Giu Asp Pi 130 Pro Giu P1 145 Thr Asp Vi Asp Gly P) Asn Lys P) Ala Leu So 210 Gin Leu A! 225 <210> 767 <211> 513 <212> DNA Leu Giu Gin Val Thr Gin 100 Ser Val1 Thr Asp Val1 180 Tyr Gin Ala 5 Leu Phe Vai Thr Arg Giu Asp Ser Gly Leu 165 Ala Asp Glu Ala Gin Leu 55 Gly Gly Val1 Arg Ile 135 Ile Lys Leu Asp Lys 215 Ile Leu Ile Val Leu Ser 105 Arg Thr Tyr Gly Pro 185 Giu Ile His Lys Gin Asp Asp Thr Thr Ala Giy Ile Met 170 Gly Vai Thr Val Arg Ser Ile Phe Met Pro Leu Arg Gly 155 Asn Ser Vai Asp Gly Ser Ser Leu Gly Thr Gly Phe Ala 140 Gin Ala Ala Ala Al a 220 Ser Asn Val Asn Ala Asp Asn Ser 125 Ser Giu Ala Ala Ala 205 Giy <213> Corynebacteriun giutamicum 1067 WO 01/00843 <220> <221> CDS <222> (490) <223> RXA01516 <400> 767 tctgcacata tgggagcatg gggtgtgcgc gtgcacgatg PCTIBOOIOO923 tcccagtatc aagggacgct gttgatgttg ccgcattgtg gcgaagtgga ggaactcacc atg gct gat cgt att Met Ala Asp Arg Ile gaa Giu gaa Glu gat Asp tac Tyr agg Arg gc t Al a aaa Lys cgt Arg ctt Leu aaa Lys ttc Phe ggc Gly gat Asp aaa.

Lys gca Ala tcc Ser aaa Lys gag Giu gat Asp gcg Ala ttg Leu ttt Phe ccg Pro agg Arg ggc ctt gaa tgc Gly Leu Glu Cys caa ggc cag ccc Gin Giy Gin Pro gcc gca ggt gcc Ala Ala Gly Ala ttg gca ttg ttg Leu Ala Leu Leu 60 atc gag acg gtg Ile Glu Thr Val 75 gat gcg ctt cat Asp Ala Leu His atc cca cgt act Ile Pro Arg Thr 105 aaa tcc atg gct Lys Ser Met Ala ttc Phe t tc Phe agc Ser 45 gtt Val gcc Ala gcg Ala ttt Phe gga Gly att Ile 30 gat Asp gct Ala acg Thr gtg Val1 gct Ala 110 1 ggt Gly gtc Val1 tcc Ser gtg Val1 65 gcg Ala ac c Thr gcg Ala gac Asp 20 tgg Trp gta Val1 cca Pro gtg Val aag Lys 100 gcc Ala ttt Phe atg Met ga t Asp t cc Ser a tg Met ccc Pro cga Arg 163 211 259 307 355 403 451 500 gct gga agg agc aac gcc taatgcatgc Ala Gly Arg Ser Asn Ala 125 130 120 agttttgtcc atc <210> 768 <211> 130 <212> PRT <213> Corynebacterium giutamicwn <400> 768 Met Ala Asp Arg Ile Giu Leu Lys Gly Leu Glu Cys Phe Gly His His 1 5 10 Gly Val Phe Asp Phe Glu Lys Giu Gin Gly Gin Pro Phe Ile-Val Asp 25 Val Thr Cys Trp Met Asp Phe Asp Ala Ala Gly Ala Ser Asp Asp Leu 40 1068 WO 01/00843 WO 0100843PCTIiBOO/00923 Ser Asp Thr Val Asp Tyr Gly Ala Leu Ala Leu 55 Val Glu Gly Pro Ser Arg Asp Leu Ile Giu Thr 70 Ala Asp Ala Val Met Ala Lys Phe Asp Ala Leu 90 Thr Ile His Lys Pro Lys Ala Pro Ile Pro Arg 100 105 Ala Val Val Ala Arg Arg Ser Arg Lys Ser Met 115 120 Asn Ala 130 <210> 769 <211> 975 <212> DNA <213> Corynebacterium giutanicun <220> <221> CDS <222> (101)..(952) <223> RXA015i5 <400> 769 taagcctggt gctgtgacca cgacgtctgc ggtgcgcggc ctcccgcgct gaggtgttct ccctgattcg ggggcactaa Leu Val1 His Thr Ala Ala Glu Thr Giu Val Giu Ala Asp 110 Giy Arg t tg Leu gag Giu atc I le gtC Val gtg Val1 ggc Gly ccg Pro tt t Phe gcc Al a gag Giu gac Asp act Thr tgt Cys ggt Gly ttg Leu Cgg Arg gtg Val1 gac Asp ttg Leu 9gC Gly gtg Val cct Pro ccg Pro acc Thr gtc Val aag Lys 30 gc t Ala 9gg Giy gtc Val atg Met atg Met tac Tyr gct Ala gca Ala att Ile cgg Arg ggttttaaga acaacgctgc atg aac gta tcc tct Met Asn Vai Ser Ser 1 att gtc aat gtc act Ile Vai Asn Val Thr gac gtt gat cag gcg Asp Val Asp Gin Ala gcc gac atg att gat Ala Asp Met Ile Asp cgc gtc gac gcg tcc Arg Val Asp Ala Ser gcg ctt cac gac gcc Ala Leu His Asp Ala tcc gtg gcg cag gct Ser Val Aia Gin Ala 100 115 163 211 259 307 355 403 1069 WO 01/00843 WO 0100843PCTIIBOO/00923 gcc Ala gc t Ala tgt Cys gca Ala 150 gat Asp gtg Val cg t Arg ctg Leu cgt Arg 230 gtg Val gat: Asp agt Ser gcg Ala gat Asp ttg Leu 135 gat: Asp ct t Leu ctt Leu tt g Leu gtg Val 215 ggc Gly acc Thr gtc Val gga Gly ggc gct Gly Ala 105 ccl: gag Pro Glu 120 atg cac Met His cac ggt His Gly gtC gcc Val1 Ala gat: cca Asp Pro 185 ctg caa Leu Gin 200 gga gca Gly Ala cta gat: Leu Asp gca gtg Ala Val cca gta Pro Val 265 gga act Giy Thr 280 ggc gtc tcc atg atc aac gac gtc tcl: Giy Val Ser Met Ile Asn Asp Val Ser 110 tltt Phe cgc Arg gac Asp 155 gcc Ala ttg Leu ctg Leu cgg Arg acc Thr 235 gca Ala agg Arg tc t Ser ac c Thr 140 gtt Vai acc Thr ggt Gly ccc Pro aag Lys 220 ccc Pro cat His gac Asp gtC Val 125 ctc Leu gta Val1 gc t Ala ttt Phe gag Giu 205 cga Arg att Ile atg Met gct a tg Met caa Gin gcc Ala gct Ala gcc Ala 190 ttt Phe ttc Phe gat Asp gga Gly gtt gcg Ala t tc Phe gat Asp ggt Gly 175 aaa Lys att Ile ctg Leu gcc Ala gca Ala 255 gal: gaa Glu ggt Gly gtg Val 160 g tg Val1 tca Ser tc t Ser gct Aia gac Asp 240 tgg Trp gtt gcg Ala gat: Asp 145 cac His gcc Ala cgt Arg gga Gly ggc Gly 225 cca Pro ggt Gly gcc ggc ggt Gly Giy 115 altt ccc Ile Pro gca ggt Ala Gly gtg ctt Vai Leu aac cag Asn Gin 180 gac aac Asp Asn 195 ttc ccc Phe Pro cgc aaa Arg Lys acc gca Thr Ala cgc gtg Arg Vai 260 ttg tgg Leu Trp t tg Leu gtg Val cag Gin ga: Asp 165 atc Ile tgg Trp atc Ile gac Asp gcg Ala 245 cac His cga Arg 499 547 595 643 691 739 787 835 883 931 Ala Val Asp Vai Ala Ala cac cat ggc His His Giy tgatcgtatt gaacttaaag gcc 770 <2i1> 284 <2i2> PRT <2i3> Corynebacterium giutanicum <400> 770 Mel: Asn Val Ser Ser Leu Thr Ile Pro Giy Arg Cys Leu Val Mel: Gly 1 5 10 Ile Val Asn Val Thr Giu Asp Ser Phe Ser Asp Gly Gly Lys Tyr Ile 25 1070 WO 01/00843 Asp Val Asp Ala Asp Met Arg Val Asp Ala Leu His Ser Val Ala Val Ser Gly 115 Ala Gln Ile 130 Asp Ala Ala 145 His Ala Val Ala Giu Asn Arg Giu Asp 195 Gly Pro Phe 210 Gly Val Arg 225 Pro Ala Thr Gly Val Arg Ala Ala Leu 275 Gin Ile Ala Asp Gin 100 Gly Pro Gly Leu Gin 180 Asn Pro Lys Ala Val 260 Trp Ile Val Val 70 Gly Ala Ala Cys Ala 150 Asp Val Arg Leu Arg 230 Val

ASP

Ser Ala Gly 55 Giu Ile Ala Asp Leu 135 Asp Leu Leu Leu Val 215.

Gly Thr Val Gly His Gly Arg His Gly Pro 120 Met His Val Asp Leu 200 Gly Leu Ala Pro Gly 280 Ala Glu Asp Thr Ala 105 Giu His Gly Ala Pro 185 Gin Al a Asp Val Val1 265 Thr Lys Ser Arg Ser Gly Met Trp Gly Arg 170 Gly Ala Ser Val Ser 250 Ser His Glu Thr Val1 75 Val1 Val Phe Arg Asp 155 Ala Leu Leu Arg Thr 235 Ala Arg His Leu Arg Val Asp Ser Ser Thr 140 Val1 Thr Gly Pro Lys 220 Pro His Asp Gly Ala Gly Val Met Ile 110 Met Gin Ala Ala Ala 190 Phe Phe Asp Gly Val 270 PCT/IBOO/00923 Ala Gly Ala Val Ile Lys Arg Ala Asn Asp Ala Glu Phe Gly Asp Val 160 Gly Val 175 Lys Ser Ile Ser Leu Ala Ala Asp 240 Ala Trp 255 Asp Val <210> 771 <211> 859 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(859) <223> RXA02024 <400> 771 cactgatgac ctggatcagg ccgtcaaatt catcgtcgat gcacacgctg gattggacgt WO 01/00843 WO 0100843PCTJIBOOIOO923 agcgcgtctc cacaattaag cagtggctac attaggtgtt atg agt tct ttg Met Ser Ser Leu gtc atc atg gcc atc gtc aat cgc acc Val Ilie Met Ala Ile Vai Asn Arg Thr ggt gcg aca ttt gag gac acc gct gcg Gly Ala Thr Phe Giu Asp Thr Ala Ala 30 gat tct ttc tat Asp Ser Phe Tyr gac aag Asp LYS 115 163 211 259 cta aac agg gca gcg gag gtc Leu Asn Arg Ala Ala Giu Val att gaa caa Ilie Giu Gin ggc gcc ggc att Gly Ala Gly Ilie gat atc ggt ggg Asp Ilie Gly Gly aaa gcc ggc Lys Ala Gly ccg ggg Pro Gly gat ttc gtg tcg Asp Phe Val Ser gag gaa gag atc Giu Glu Giu Ile cgc gtg gtg cca Arg Val Val Pro atc Ilie atc gct gcg gtg Ilie Ala Ala Val gaa cgt ttt cct Giu Arg Phe Pro gac Asp att gat att tct Ile Asp Ile Ser gat acc tgg cgg Asp Thr Trp Arg tcg gtg gct gat Ser Val Ala Asp gca gtg gcg cat Ala Val Ala His gga gca Gly Ala 100 acg ctg atc Thr Leu Ile gta gca ggg Val Ala Gly 120 aat Asn 105 gac act tgg gcc Asp Thr Trp Ala ggc cat gat cat gag ttg gtg cag Gly His Asp His Giu Leu Val Gin 110 115 tat gtc tgc tcg cac acc ggc ggg Tyr Val Cys Ser His Thr Gly Giy 130 cag cac aag gtg Gin His Lys Val gtg att Val Ile 135 cca aga acg cga cca tat cgg gtg cat Pro Arg Thr Arg Pro Tyr Arg Val His ttc Phe 145 gat gac atc gtg Asp Asp Ilie Val gcc Ala 150 gat gta att acg gag acc acc aaa ttg Asp Vai Ilie Thr Giu Thr Thr Lys Leu 155 gca Ala 160 gag caa gct gtt Glu Gin Ala Val gcc ggg gtg cca Ala Giy Vai Pro gag Giu 170 gaa cgg gtg ttt att gat ccc acc cat Giu Arg Val Phe Ilie Asp Pro Thr His 175 gat ttc Asp Phe 180 ggg aaa aac Gly Lys Asn gtg gtt gcc Val Val Ala 200 acc Thr 185 ttc cac gga ctg Phe His Giy Leu ctt tta cga cgg Leu Leu Arg Arg atc gat gag Ile Asp Giu 195 aat aag gat Asn Lys Asp acg ggc tgg ccg Thr Gly Trp Pro gtg Vai 205 ctg atg gcc ttg Leu Met Ala Leu agt Ser 210 ttc att Phe Ile 215 ggg gaa act ttg Gly Giu Thr Leu gaa Giu 220 agg ggc gtc gat Arg Gly Val Asp aag Lys 225 cgt gtt gct ggc Arg Val Ala Gly 1072 WO 01/00843 acg ctt gct Thi- Leu Ala 230 cgc gtg cat Arg Val His gcc act Ala Thr gaa gtt Glu Val 250 PCTILBOO/00923 gcc tgg gcg gcg gcg cgc ggc gtt gcg gct ttt 835 Ala T-p, Ala Ala Ala Arg Gly Val Ala Ala Phe 235 240 245 gcg gaa acc 859 Ala Glu Thi- <210> 772 <211> 253 <212> PRT <213> Corynebacterium giutamicum <400> 772 Met Ser Ser Leu Pro Val Ile Met Ala 1 S er Arg Gly Asp Ile Val1 His Ser Phe 145 Glu Pro Arg Leu Lys 225 Giy Phe Ala Val Arg Asp Ala Glu His 130 Asp Gin Thi- Arg Ser 210 Arg Val Tyr Al a Lys Val1 Ile His Leu 115 Thr Asp Ala His Ile 195 Asn Val Ala Gly Ile Pro Ile 70

ASP

Thi- Val Val Ala 150 Ala Gly Val Phe Thi- 230 Arg Al a Glu Gly Ile Thi- Leu Ala Ile 135 Asp Gly Lys Val Ile 215 Leu Val1 Thi- Gin Asp Ala Ti-p Ile Gly 120 Pro Val Val Asn Ala 200 Gly Ala His Phe Gly Phe Ala Arg Asn 105 Gin Arg Ile Pro Thi- 185 Thr Giu Ala Glu Ile 10 Giu Ala Val Val1 Ala Asp His Thi- Thr Glu 170 Phe Gly Thi- Thr Val1 Val Asp Gly Ser Arg 75 Ser Thi- Lys Arg Glu 155 Giu His Ti-p Leu Ala 235 Al a Th- Ala Asp Giu Phe Asp Gly 110 Tyr Arg Lys Phe Giu 190 Leu Gly Ala Pro Leu Ile Giu Pro Val His Val1 Val Leu Ile 175 Leu Met Val Ala Asp Asn Gly Ile Asp Ala Asp Cys His Ala 160 Asp Leu Ala Asp Arg 240 1073 WO 01/00843 WO 0100843PCTA~BOO/00923 245 <210> 773 <211> 684 <212> DNA <213> Corynebacteriun giutainicum <220> <221> <222> <223>

CDS

(101) (661) RXA01719 <400> 773 ccatatggtc ggtgcactgg ctggaagtgg cggtattgca ctggtgactt cgtcgattgc cgaggacggt ctggttgatg ttgtgctggg gagaatgtaa atg aat atc atc att Met Asn Ile Ile Ile 115 ctt gct ggt ggc Leu Ala Gly Gly ggt aaa cgc atg Gly Lys Arg Met ggt Gly ggg gtg gat aag Gly Val Asp Lys gct gct Ala Ala gtg gcg gtg gat ggt cgc acg ctg Val Ala Val Asp Gly Arg Thr Leu gat cca gaa gat gac gtc gtg gtg Asp Pro Giu Asp Asp Val Val Val gat atc ctg ctt Asp Ile Leu Leu tca cag. ctg Ser Gin Leu atc gac gga Ile Asp Gly gtt tcc ccc gcg Val Ser Pro Ala atc acg Ile Thr act gtc tgc gag Thr Val Cys Giu cct ccg ctt ggc Pro Pro Leu Gly ccg gtc gcg gga Pro Val Ala Gly gag gca ggg ctg Giu Ala Gly Leu tct ttt gag cac Ser Phe Giu His gc c Ala cat gaa ttc act His Giu Phe Thr att ctt gcc gtg Ile Leu Ala Val gcg cct tat tct Ala Pro Tyr Ser gca Ala gcg atg ctg ccc Ala Met Leu Pro cta ctt Leu Leu 100 cag gca cag Gin Ala Gin ggc tgg gta Gly Trp, Val 120 ggc aaa gcc gat Gly Lys Ala Asp gcc gta acc ctt gct gcc gat Ala Val Thr Leu Ala Ala Asp 115 caa ccg ttg tgc Gin Pro Leu Cys ctg tgg aga agt Leu Trp Arg Ser agc cta gaa Ser Leu Giu gcg gtg Ala Val 135 att cac agc ctg ggc gag act aga aat cga ccg gca aaa gcg Ile His Ser Leu Gly Giu Thr Arg Asn Arg Pro Ala Lys Ala 140 145 ctg aag caa gcg Leu Lys Gin Ala cac att gtg gaa His Ile Val Giu gtg Val1 160 ggt ggc gat ggc Gly Gly Asp Gly gaa aaa gac tac gat acg gtg gct gaa ctg gag gta ttg ggc aac gta 1074 WO 01/00843 PCT/IBOO/00923 Glu Lys Asp Tyr Asp Thr Val Ala Giu Leu Glu Val Leu Gly Asfl Val 170 175 180 acg ctc cct aaa gcc cac tgatgagaaa cgtgagctct gct 684 Thr Leu Pro Lys Ala His 185 <210> 774 <211> 187 <212> PRT <213> Corynebacterium giutainicum <400> 774 Met Asn Ile Ile Ile Leu Ala Giy Gly 1 Val Leu Ala Gly His Met Thr Ser Arg 145 Giy Val1 Lys Ser Ile Val Phe Pro Ala 115 Ser Ala Asp Giy Ala Gin Asp Ala Thr Leu 100 Ala Leu Lys Gly Asn 180 Val1 Asp Ile Ile 70 Ile Gin Gly Ala Leu 150 Glu Thr Ala Pro Thr Glu Leu Ala Trp Val 135 Leu Lys Leu Val Giu Thr Ala Ala Gin Val 120 Ilie Lys Asp Pro Asp 25 Asp Val Gly Val Ile 105 Gin His Gin Tyr Lys 185 Giu 10 Gly Asp Cys Leu Asp Gly Pro Ser Ala Asp 170 Ala Gly Arg Val Glu Asn 75 Ala Lys Leu Leu Gly 155 Thr His Lys Thr Val1 Glu Ser Pro Ala Cys Gly 140 His Val Met Leu Val1 Pro Giu Ser Val1 110 Leu Thr Val Giu Gly Asp Ser Leu His Ala Ala Trp Arg Giu Leu 175 Gly Ile Pro Gly Ala Ala Val Arg Asn Val1 160 Giu <210> 775 <211> 1332 <212> DNA <213> Corynebacterium glutainicum <220> <221> CDS <222> (101)..(1309) <223> RXA01720 1075 WO 01/00843 <400> 775 acaccCgttt gagaatctca acctcttaaa acagcgcgaa ctatatattt gagtgtttat tgtcgaaaaa ggggtttcaa PCTLBOO/00923 ctattgattt ggcaactacc gca cag caa cgc Ala Gin Gin Arg agc Ser c cg Pro gca Ala gc t Ala ccg Pro gcg Ala gtc Val aat Asn c ta Leu gaa Glu 150 gtg Val1 tgc Cys aat Asn cta Leu gtc Val1 cca Pro gag Giu gtt Val tgg Trp agc Ser cca Pro gc t Ala cca Pro 135 ggc Gly tcg Ser cc t Pro ccc Pro aaa Lys gac.

Asp aaa Lys gtt Val gg t Gly ttt Phe aat Asn ac c Thr 105 gtg Val gag Glu att Ile gtg Val gt t Val 185 ggt Gly gtg Val tat Tyr acc Thr gtc Val1 tac Tyr ctt Leu aa t Asn aac Asn ga t Asp agg Arg gcg Ala att Ile 170 gcg Ala atc Ile ggg Gly ctt Leu cc t Pro gcg Ala gcc Ala gtg Val1 75 gga Gly aag Lys cac Hius aat Asn gt t Val 155 tca Ser gtg Val cc t Pro att Ile tcc Ser atc Ile aca Thr att Ile 9gc Gly aaa Lys gac Asp tcg Ser aat Asn 140 gct Ala gtt Val atc Ile aat Asn aag att Ile ctc Leu att Ile ttg Leu 99t Gly gcc Ala atg Met ctt Leu 125 at t Ilie gcg Ala ggc Gly act Thr t cc Ser 205 ga t ttg Leu ggC Gly 30 cct Pro aaa Lys gat Asp atc Ile atc Ile 110 cc t Pro cgc Arg ggg Gly cat His ac c Thr 190 aa t Asn ccg ata Ile 15 gcg Ala atc Ile gaa Giu act Thr cgt Arg 95 gtg Val1 aca Thr cat His acg Thr gac Asp 175 ggt Gly ggg Gly cag Gin gca Al a agt Ser 35 act Thr ggc Gly tc t Ser ggt Gly gag Giu 115 atc Ile cat His gcg Ala gcc Ala aac Asn 195 gtt Val tcc Ser ccg Pro 20 cac His aat Asn agc Ser gcg Ala gga Gly 100 c tc Leu aat Asn ctt Leu ggt Gly cat His 180 cag Gin gcg Al a gat Asp ctt Leu ctt Leu tct Ser 9gg Gly ccg Pro ccc Pro acc Thr gag Giu aaa Lys act Thr 165 cct Pro gga Gly gag Giu gat Asp 115 163 211 259 307 355 403 451 499 547 595 643 691 739 787 Lys Asp Pro 1076 WO 01/00843 215 tct gag act Ser Giu Thr 230 gat gtg atc Asp Val Ilie gtc aaa gcc Val Lys Ala gcg atg aag Ala Met Lys 280 aaa gtg gtg 1027 Lys Vai Val 295 agg ctc ttt 1075 Arg Leu Phe 310 ctg gcg tcg 1123 Leu Ala Ser gcg ctg aag 1171 Ala Leu Lys gat tgg gag 1219 Asp Trp, Glu 360 gtc ggt gca 1267 Val Gly Ala 375 att gcc gag 1309 PCT/IBOO/00923 ttg Leu act Thr 250 gga Gly ggt Gly ctg Leu gt t Vai agc Ser 330 cgg Arg aga Arg gc t Ala ggt agg Arg 235 gtg Val act Thr aaa Lys ccg Pro ccg Pro 315 gaa Giu gag Giu atg Met gga Gly ctg 220 gag Giu ggt Gly aag Lys ccg Pro gga Gly 300 gta Val ctc Leu ggc Gly gca Ala agt Ser 380 gtt acg Thr ggg Gly act Thr c aa Gin 285 aac Asn att Ile cc t Pro ccc Pro aa t Asn 365 ggc Gly gat Asp gca Ala gcg Ala ttc Phe tgg Trp 290 tgg Trp gg t Gly cgc Arg ccg Pro cga Arg 370 gtg Val aga Arg gag Giu t tc Phe t tC Phe 275 ggc Gly gtt Val gga Gly tcg Ser gtg Val1 355 tcc Ser act Thr atg Met Ilie Ala Giu Asp Gly Leu Val 400 taaatgaata tcatcattct tgc 1332 <210> 776 <211> 403 <212> PRT <213> Corynebacteriun giutainicum 1077 WO 01/00843 PCT/IB00/00923 <400> 776 Val Ala Gin Gin Arg Ser Val Asp Asp Tyr Leu Ser Ile Leu Ile Asp 1 5 10 Ser Val Ala Pro Leu Pro Pro Val Lys Thr Pro Ile Leu Gly Ala His 25 Pro Leu Ser His Leu Ala Glu Asp Val Val Ala Thr Ile Pro Ile Pro 40 Lys Phe Thr Asn Ser Ala Val Asp Gly Tyr Ala Ile Leu Lys Glu Asp 55 Ile His Gly Ser Gly Pro Trp Thr Phe Leu Val Gly Gly Asp Thr Pro 70 75 Ala Gly Ser Ala Pro Ala Ser Ile Asn Asn Gly Lys Ala Ile Arg Val 90 Met Thr Gly Gly Pro Val Pro Ser Thr Asn Lys Asp Met Ile Val Val 100 105 110 Pro Val Glu Leu Thr Asn Ala Pro Val Asp His Ser Leu Pro Thr Glu 115 120 125 Ile Thr Ile Asn Glu Leu Pro Gly Glu Arg Asn Asn Ile Arg His Ala 130 135 140 Gly Glu His Leu Lys Glu Gly Glu Ile Ala Val Ala Ala Gly Thr Ala 145 150 155 160 Phe Asp Ala Gly Thr Val Ser Thr Val Ile Ser Val Gly His Asp Thr 165 170 175 Val Lys Ala His Pro Cys Pro Arg Val Ala Val Ile Thr Thr Gly Asp 180 185 190 Glu Leu Asn Gin Gly Asn Pro Trp Gly Ile Pro Asn Ser Asn Gly Pro 195 200 205 Met Leu Val Ala Glu Leu Lys Arg Val Gly Ile Lys Asp Pro Gin His 210 215 220 Phe His Ser Asp Asp Ser Glu Thr Ala Leu Arg Glu Thr Leu Asp Lys 225 230 235 240 Pro Ala Glu Val Ala Asp Val Ile Ile Thr Val Gly Gly Ile Ser Ala 245 250 255 Gly Ala Phe Asp Val Val Lys Ala Val Gly Thr Lys Thr Gly Gly Phe 260 265 270 Glu Phe Phe Pro Ile Ala Met Lys Pro Gly Lys Pro Gin Gly His Gly 275 280 285 Gin Trp Gly Asp Ala Lys Val Val Cys Leu Pro Gly Asn Pro Val Ala 290 295 300 Ala Trp Val Ser Phe Arg Leu Phe Val Val Pro Val Ile Glu Arg Leu 305 310 315 320 1078 WO 01/00843 WO 0100843PCTIIBOO/00923 Gly Leu Ile His Leu 385 Gly Lys Arg 325 Asn Arg 340 Ala Ile His Met Ser Ser Leu Ala Asp Val Ile 390 Ala Ser Ile Ser 330 Leu Lys Ala Arg 345 Trp, Giu Lys Arg 360 Gly Ala Leu Ala 375 Ala Giu Asp Gly Leu Pro Val Val 335 Gly Pro Val Leu 350 Ala Asn Ser Gin 365 Ser Gly Gly Ile 380 Val Asp Val Val <210> 777 <211> 1237 <212> DNA <213> Corynebacterium glutanicun <22 0> <221> CDS <222> (1207) <223> RXS03223 <400> 777 tcca gag cca gtg cgt att gct att gca gag gca ctg ggt ttg atg tgc Pro Giu Pro Val Arg Ilie Ala Ilie Ala Giu Ala Leu Giy Leu Met Cys 1 5 10 gcg gaa gag gtt caa gct agt cgt gct ttg ccg ttc gcg caa Ala gcg Ala tcg Ser gtg Vai cct Pro gcg Ala gta Val Ala gcg Ala c tg Leu gc t Ala atg Met ccc Pro cci: Pro Ala ggc Gly tcc Ser cgc Arg ccg Pro cgc Arg 110 cgc Arg Gin gag Giu ctg Leu ctg Leu atg Met aaa Lys aaa Lys 1079 WO 01/00843 WO 0100843PCTJIBOO/00923 ggc gat Giy Asp 130 gac atc caa ccg Asp Ile Gin Pro gac atc gca gtc age gcc ggc gcg gte Asp Ile Ala Val Ser Ala Gly Ala Val 140 t ta Leu 145 ggc cct gee eaa Giy Pro Ala Gin ggt ttg etc gca Gly Leu Leu Ala gc t Ala 155 gtt ggt cge tee Val Gly Arg Ser gtg ttg gtg tac Val Leu Val Tyr cgc cca ege atg Arg Pro Arg Met gtt atc tee gta Val Ile Ser Val ggc get Gly Ala 175 gaa ett gtt Giu Leu Val gtc aat tee Val Asn Ser 195 gat Asp 180 att gat cgc cag Ile Asp Arg Gin ca Pro 185 ggc etc ggc cag Gly Leu Giy Gin gtt tat gat Vai Tyr Asp 190 ggc gca gat Giy Aia Asp tat tct ctg get Tyr Ser Leu Aia gcc ggt agg gaa Aia Gly Arg Giu gtg tac Val Tyr 210 ege tac gge att Arg Tyr Giy Ile gec ggt gaa ect Ala Giy Giu Pro cgt Arg 220 ege atc aaa gag Arg Ilie Lys Giu ate Ilie 225 att gaa tee eag Ile Giu Ser Gin etg ege tcg gaa Leu Arg Ser Giu ate gte ate ace Ile Val Ile Thr get gtt ggc ggt get ggt tca get ggc Ala Vai Giy Giy Ala Gly Ser Ala Gly 245 cgc cag gtt etc Arg Gin Val Leu aac gag Asn Giu 255 eta gge gat Leu Gly Asp caa gga tte Gin Gly Phe 275 atc Ile 260 gac ace gaa cgc Asp Thr Giu Arg gca atg cae ccc Ala Met His Pro ggt tet gtc Gly Ser Val 270 tte ett etg Phe Leu Leu ggt etg ctc ggc Gly Leu Leu Gly aac aag att cca Asn Lys Ile Pro tgc Cys 285 cet tee Pro Ser 290 aat ccg gtg geg tcg tta gtt att ttt Asn Pro Val Ala Ser Leu Val Ile Phe 295 acc ttc gtc cgc Thr Phe Val Arg gte gtg cgc atg Val Val Arg Met age Ser 310 ctg ggc aag agc Leu Gly Lys Ser geg gcg cgc cgg Ala Ala Arg Arg gtt ega 1009 Val Arg ggt ttc 1057 Gly Phe gct ega gcg etc aac eac gtt gtg tct gtg gcg ggt cga aaa Ala Arg Ala 325 Leu Asn His Val Val Ser Val Ala Gly Arg Lys 335 ate agg tee agg etc atg cge gat gca gaa acc cag gac tac Ile Arg 340 Ser Ax-g Leu Met Asp Ala Giu Thr Gin Asp Tyr 350 1080 WO 01/00843 ctc gtg gag gct ttg ggt ggt gca acg ggc, 1105 Leu Val Glu Ala Leu Gly Gly Ala Thr Gly, 355 360 gca gga ttg tcc gaa gca aac ggt atg atc, 1153 Ala Gly Leu Ser Glu Ala Asn Gly Met Ilie, 370 375 aca gaa atc cga ccg gga gat gtc gtg gac 1201 Thr Giu Ile Arg Pro Gly Asp Val Val Asp' 385 390 ggt cga tagttcgatg cgtaatgcac cgtcaggtcc 1237 Gly Arg PCTIBOO/00923 cca tcg cac cta ttg Pro Ser His Leu Leu 365 att cca gaa gat gtc Ile Pro Giu Asp Val 380 atc ttc ctt gcc caa Ile Phe Leu Ala Gin 400 <210> 778 <211> 402 <212> PRT <213> Corynebacterium glutamicum <400> 778 Pro Giu Pro Val Arg Ile Ala Ilie Ala i Ala Ala Ser Val Pro Ala Val Gly Leu 145 Val Glu 5 Gln Tyr Gln Val Val1 Leu Arg Gin Gin Pro 165 Ile Al a Ala Leu Ala Met Pro Pro Pro Ile 150 Arg Asp Glu 10 Leu Val Pro Gin Giy 90 Ser Gly Al a Ala Ser 170 Gly Leu Gly Val Giu Pro Pro Arg Phe Ser 140 Val Ile Gly Leu Al a Gly Ser Arg Pro Arg 110 Arg Gly Arg Val Val Met is Gln Giu Leu Leu Met Lys Lys Ala Ser Gly 175 Tyr WO 01/00843 WO 0100843PCTIiBOO/00923 180 185 190 Val Asn Ser Tyr Ser Leu Ala Ala Ala Gly Arg Glu Ala Gly Ala Asp 195 200 205 Val Tyr Arg Tyr Gly Ilie Ala Ala Gly Glu Pro Arg Arg Ilie Lys Giu 210 215 220 Ile Ilie Glu Ser Gin Met Leu Arg Ser Giu Ile Ile Val Ile Thr Gly 225 230 235 240 Ala Val Gly Gly Ala Gly Ser Ala Gly Val Arg Gin Val Leu Asn Giu 245 250 255 Leu Gly Asp Ilie Asp Thr Giu Arg Vai Ala Met His Pro Gly Ser Val 260 265 270 Gin Gly Phe Gly Leu Leu Gly Glu Asn Lys Ile Pro Cys Phe Leu Leu 275 280 285 Pro Ser Asn Pro Val Ala Ser Leu Val Ile Phe Giu Thr Phe Val Arg 290 295 300 Pro Val Val Arg Met Ser Leu Gly Lys Ser Asn Ala Ala Arg Arg Val 305 310 315 320 Val Arg Ala Arg Ala Leu Asn His Val Val Ser Val Ala Gly Arg Lys 325 330 335 Gly Phe Ilie Arg Ser Arg Leu Met Arg Asp Ala Glu Thr Gin Asp Tyr 340 345 350 Leu Val Glu Ala Leu Gly Gly Ala Thr Gly Ala Pro Ser His Leu Leu 355 360 365 Ala Gly Leu Ser Glu Ala Asn Giy Met Ile Arg Ile Pro Glu Asp Val 370 375 380 Thr Giu Ilie Arg Pro Gly Asp Val Val Asp Val Ilie Phe Leu Ala Gin 385 390 395 400 Gly Arg <210> 779 <211> 1229 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (1)..(1206) <223> FRXA01970 <400> 779 cca gag cca gtg cgt att gct att gca gag gca ctg ggt ttg atg tgc 48 Pro Giu Pro Val Arg Ilie Ala Ile Ala Glu Ala Leu Gly Leu Met Cys 1 5 10 gcg gaa gag gtt caa gct agt cgt gct ttg ccg ggt ttc gcg caa gca 96 1082 WO 01/00843 Ala Glu Giu Val Gin Ala Ser Arg Ala Leu Pro Gly Phe Ala PCT/IBOO/00923 25 geg Ala teg Ser gtg Val1 CC t Pro geg Ala gta Val gge Gly t ta Leu 145 gtg Val gaa Giu gtc Val gtg Val ate Ile 225 get Ala cta Lau gat Asp agc Ser ggt Gly eaa Gin geg Ala geg Ala 115 gae Asp cc t Pro gtg Val gt t Val1 tee Ser 195 egc Arg gaa Glu gge Gly ga t Asp ggt Gly eag Gin gaa Glu gea Ala g tg Val 100 eag Gin ate Ilie gee Ala tae Tyr gat Asp 180 tat Tyr tae Tyr tee Ser ggt Gly ate Ile tat Tyr eaa Gin gta Val1 gte Val etg Leu ega Arg eaa Gin eaa Gin e ea Pro 165 att Ile tet Ser 9ge Gly eag Gin get Ala 245 gac Asp gtt Val ceg Pro geg Ala gte Val atg Met gtg Val1 gga Gly 135 ggt Gly eca Pro egc Arg get Ala get Ala 215 e tg Leu tea Ser gaa Glu ega gea Arg Ala 40 gtt get Val Ala ggt tet Gly Ser eae ace His Thr geg tgg Ala Trp 105 ege tet Arg Ser 12.0 gac ate Asp Ile ttg etc Leu Leu ege atg Arg Met eag eca Gin Pro 185 gee gee Ala Ala 200 gee ggt Ala Gly ege teg.

Arg Ser get gge Ala Gly ege gte Arg Val gte Val1 ect Pro eag Gin gg t Gly 90 tea Ser ggC Gly gea Al a gca Ala teg Ser 170 gge Gly ggt Gly gaa Giu gaa Giu gtg Val 250 gea Ala gat gte Asp Val ceg gaa Pro Giu 60 eag ceg Gin Pro 75 geg eca Ala Pro gat egt Asp Arg gag ttt Giu Phe gte age Val Ser 140 get gtt Ala Val 155 gtt ate Val Ilie etc ggc Leu Gly agg gaa Arg Giu cet egt Pro Arg 220 ate ate Ile Ile 235 ege eag Arg Gin atg eac Met His gge Gly 45 aaa Lys t tg Leu e tg Leu gge Gly gtg Val1 125 gee Ala ggt Gly tee Ser eag Gin geg Ala 205 ege Arg gte Val gt t Val1 ec Pro gge Gly tee Ser ege Arg ceg Pro ege Arg 110 ege Arg gge Gly ege Arg gta Val gtt Val 190 gge Gly ate Ile ate Ile etc Leu ggt Gly Gin gag Glu c tg Leu ctg Leu atg Met aaa Lys aaa Lys geg Ala tee Ser gge Gly 175 tat Tyr gea Ala aaa Lys ace Thr aac Asn 255 tet Ser Ala aag Lys ccc Pro cag Gin ett Leu ega Arg gaa Giu gte Val aaa Lys 160 get Ala gat Asp gat Asp gag Glu gga Gly 240 gag Giu gte Val 144 192 240 288 336 384 432 480 528 576 624 672 720 768 816 1083 WO 01/00843 WO 0100843PCTIBOOIOO923 260 265 270 caa gga ttc ggt ctg ctc ggc gag aac aag att cca tgc ttc Ctt ctg 864 Gin Gly Phe Gly Leu Leu Gly Giu Asn Lys Ile Pro Cys Phe Leu Leu 275 280 285 cct tcc aat ccg gtg gcg tcg tta gtt att ttt gaa acc ttc gtc cgc 912 Pro Ser Asn Pro Val Ala Ser Leu Val Ilie Phe Giu Thr Phe Val Arg 290 295 300 ccg gtc gtg cgc atg agc ctg ggc aag agc aat gcg gcg cgc cgg gtt 960 Pro Val Val Arg Met Ser Leu Gly Lys Ser Asn Ala Ala Arg Arg Val 305 310 315 320 gtt cga gct cga gcg ctc aac cac gtt gtg tct gtg gcg ggt cga aaa 1008 Val Arg Ala Arg Ala Leu Asn His Val Vai Ser Val Aia Gly Arg Lys 325 330 335 ggt ttc atc agg tcc agg ctc atg cgc gat gca gaa acc cag gac tac 1056 Giy Phe Ile Arg Ser Arg Leu Met Arg Asp Aia Glu Thr Gin Asp Tyr 340 345 350 ctc gtg gag gct ttg ggt ggt gca acg ggc gca cca tcg cac cta ttg 1104 Leu Val Glu Aia Leu Gly Giy Ala Thr Gly Ala Pro Ser His Leu Leu 355 360 365 gca gga ttg tcc gaa gca aac ggt atg atc cgc att cca .gaa gat gtc 1152 Ala Gly Leu Ser Glu Ala Asn Gly Met Ilie Arg Ile Pro Giu Asp Val 370 375 380 aca gaa atc cga ccg gga gat gtc gtg gac gtg atc ttc ctt gcc caa 1200 Thr Giu Ile Arg Pro Gly Asp Val Val Asp Val Ile Phe Leu Ala Gin 385 390 395 400 ggt cga tagttcgatg cgtaatgcac cgt 1229 Gly Arg <210> 780 <211> 402 <212> PRT <213> Corynebacterium giutamicui <400> 780 Pro Glu Pro Val Arg Ile Ala Ile Ala Giu Ala Leu Gly Leu Met Cys 1 5 10 Ala Glu Giu Val Gin Ala Ser Arg Ala Leu Pro Gly Phe Ala Gin Ala 25 Ala Ilie Asp Gly Tyr Ala Val Arg Ala Val Asp Val Gly Gly Giu Lys 40 Ser Phe Ser Gin Gin Leu Pro Val Ala Pro Pro Glu Lys Ser Leu Pro 1084 WO 01/00843 Val Val Gi Pro Lys Gi PCTIBOO/00923 Ala Vai Gly Leu 145 Val Giu Val Val Ile 225 Al a Leu Gin Pro Pro 305 Vai Gly Leu Ala Asp Thr Asp 130 Giy Leu Leu Asn Tyr 210 Ile Val Giy Giy Ser 290 Val Arg Phe Val1 Giy 370 Giu Al a Val1 100 Gin Ile Ala Tyr Asp 180 Tyr Tyr Ser Gly Ile 260 Giy Pro Arg Arg Arg 340 Ala Ser Val Val Leu Arg Gin Gin Pro 165 Ile Ser Gly Gin Ala 245

ASP

Leu Val1 Met Ala 325 Ser Leu Glu Ala 70 Met Pro Pro Pro Ile 150 Arg Asp Leu Ile Met 230 Gly Thr Leu Ala Ser 310 Leu Arg Gly Ala 55.

Ala Val Met Val1 Gly 135 Gly Pro Arg Aia Ala 215 Leu Ser Glu Gly Ser 295 Leu Asn Leu Gly Asn 375 Gly His Ala Arg 120 Asp Leu Arg Gin Ala 200 Ala Arg Ala Arg Glu 280 Leu Gly His Met Ala 360 Gly Gin Ala Asp Glu Val Ala 155 Val1 Leu Arg Pro Ile 235 Arg Met Ile Phe Asn 315 Ser Ala Ala Arg Pro Pro Arg Phe Ser 140 Val Ile Gly Glu Arg 220 Ile Gin His Pro Giu 300 Ala Val1 Glu Pro Ile 380 Leu Met Lys Lys Ala Ser Gly *17 Tyr Al a Lys Thr Asn 255 Ser Leu Val Arg Arg 335 Asp Leu Asp 1085 WO 01/00843 WO 0100843PCTIBOOOO923 Thr Glu Ile Arg Pro Gly Asp Val Val Asp Val Ile Phe Leu Ala Gin 385 390 395 400 Gly Arg <210> <211> <212> <213> <220> <221> <222> <223> 781 708

DNA

Corynebacterium giutaicurn

CDS

(685) RXA0 2629 <400> 781 tacctcgcag agccgggagg tagaggttac tctgacgagt gagtaggttt aaaagagtta atctgcatct aatcaagtag ccaagtatga gtgaggaaca atg agc aag gat cca Met Ser Lys Asp Pro 1 ttg gga agt ctt acc gat gtt gta gac Leu Gly Ser Leu Thr Asp Val Val Asp cga gtt ccg ctt Arg Val Pro Leu ccg gat Pro Asp gtt gaa ccg Val Giu Pro atg gca tcc Met Ala Ser ccg gag ttc ctg Pro Giu Phe Leu gct acg gaa aaa Ala Thr Giu Lys gaa ttc cac Giu Phe His gat cat gtc Asp His Val cag aag cgc gct ctt gtt gtc ctg gtg Gin Lys Arg Ala Leu Val Val Leu Val gct gag Ala Giu gca gat ggg act Ala Asp Gly Thr cgt ttg gtt acg Arg Leu Val Thr gag Giu ctg ctc tta gag Leu Leu Leu Giu tct ggc ttc aac gtg gac gct gtg gtc agc Ser Gly Phe Asn Val Asp Ala Val Val Ser cag att agg caa gct att gaa acc gca gtt Gin Ile Arg Gin Ala Ile Giu Thr Ala Val 95 aag tct aag aag Lys Ser Lys Lys gtt ggc ggc gct Val Gly Gly Ala gac ctt Asp Leu 100 gtg ctg acc Val Leu Thr gag gca acc Giu Ala Thr 120 atc Ile 105 ggc gga gtg Gly Gly Val ggc gtt Gly Val 110 gac cag Asp Gin 125 ggt cct cgg gat Gly Pro Arg Asp aaa act cct Lys Thr Pro 115 atc gcg cag Ile Ala Gin agc gct gtg ttg Ser Ala Val Leu gac gtc cca Asp Val Pro gga Gly 130 gcg ctt cgt tcc tcc ggt ttg gcc tgt ggc gcg gtg gat gca agt gtt Ala Leu Arg Ser Ser Gly Leu Ala Cys Gly Ala Val Asp Ala Ser Val 135 140 145 1086 WO 01/00843 PCTJIBOO/00923 tcc cga ggc gta gcg ggc gta tcc ggc tca acc gtg gtg gtc aac ctc 595 Ser Arg Gly Val Ala Gly Vai Ser Gly Ser Thr Val Val Val Asn Leu 150 155 160 165 gct gag tct cgt tcg gca att cgt gat ggc atg gca act ctg aca ccg 643 Ala Glu Ser Arg Ser Ala Ile Arg Asp Gly Met Ala Thr Leu Thr Pro 170 175 180 ttg gtt gat ttt gtt gta gat cag ctt cgc act tcc gtg gtt 685 Leu Val Asp Phe Val Val Asp Gin Leu Arg Thr Ser Val Val 185 190 195 tgagttggtc gggtgtgagt aga 708 <210> 782 <211> 195 <212> PRT <213> Corynebacteriun giutamicum <400> 782 Met Ser Lys Asp Pro Leu Gly Ser Leu Thr Asp Val Val Asp Thr 1 Val1 Glu Val Glu Lys Gly Arg Pro Val 145 Val Ala Ser Pro Lys Gly Leu Ser Gly Asp Gly 130 Asp Val Thr Val1 Leu Glu Asp Leu Lys Ala Lys 115 Ile Ala Val Leu Val 195 Pro Phe His Leu Lys Asp 100 Thr Ala Ser Asn Thr 180 5 Asp His Val Giu Ser Leu Pro Gin Val Leu 165 Pro Val Met Ala Ser 70 Gin Val Giu Ala Ser 150 Ala Leu Glu Ala Glu 55 Gly Ile Leu Ala Leu 135 Arg Gi u Val Pro Ser 40 Ala Phe Arg Thr Thr 120 Arg Gly Ser Asp Phe Ala Gly Al a Giu Val Leu Leu 140 Val1 Ile Asp Leu Leu Arg Val Thr Gly Asp 125 Ala Ser Arg Gin Lys Val Leu Val Ala Val 110 Gin Cys Gly Asp Leu 190 Ala Val Val Ser Val Gly

ASP

Gly Ser Gly 175 Arg Arg Thr Leu Thr Val Val Pro Val1 Ala Thr 160 Met Thr 1087 WO 01/00843 <210> 783 <211> 402 <212> DNA <213> Corynebacterium glutarnicum <220> <221> CDS <222> (101)..(379) <223> RX.A02318 <400> 783 PCT/iBOO/00923 gatatacgcc acggtaccgc gtacaaaatc cagtaagcgc atgtgcgcca ttttaaggce gac Asp ttt Phe gcc Ala tat Tyr acc Thr ac c Thr tcc Ser acc Thr tcc Ser ttt Phe cgt Arg gcc Ala cca Pro gga Gly gca Ala gtg Val1 gat Asp att Ile tcC Ser act Thr aac Asn 45 ctc Leu gca Ala gct Ala ccgaatatga tcatggccaa aggaggtcag agatggggcc atg aat tcg ctt ttc Met Asn Ser Leu Phe 1 gcc aac gcc aag ctc acc gca cat Ala Asn Ala Lys Leu Thr Ala His ggc atg aaa ttt gtc aac ctc att Gly Met Lys Phe Val Asn Leu Ile 30 cac cac ccc gat atc ctt ctc acc Hius His Pro Asp Ile Leu Leu Thr acc agc cac gat gtg ggt gag ata Thr Ser His Asp Val Gly Glu Ile aaa gtc atc gac gcc cac gcc aag Lys Val Ile Asp Ala His Ala Lys 80 taaggttaaa gattatgagc aac <210> 784 <211> 93 <212> PRT <213> Corynebacterium glutalnicum <400> 784 Met Asn Ser Leu Phe Asp Val Ser Pro 1 5 Lys Leu Thr Ala His Phe Asn Thr Gly 25 Phe Val Asn Leu Ile Ala Asp Ser Ala 40 Asp Ile Leu Leu Thr Tyr Gly Phe Val 55 Asp Val Gly Glu Ile Thr Asp Arg Asp His Trp Ser Ser Ala Asn Ala 10 Lys Phe Ser Thr Gly Met Lys Giu Glu Ala Asn His His Pro Glu Ile Thr Leu Thr Ser His Val Ala Leu Ala Lys Val Ile 1088 WO 01/00843 WO 0100843PCT/IBO0100923 Asp Ala His Ala Lys Thr Leu Ala Ile Ser Ala Glu Ala <210> <211> <212> <213> <220> <221> <222> <223> 785 600

DNA

Corynebacterium

'CDS

(101). .(577) RXA0 1517 giutamicum <400> 785 tccataagcc caaagcaccg atcccacgta cttttgctga cgtcgcggtg gttgcccgac gttccaggaa atccatggct gctggaagga gcaacgccta atg cat gca gtt ttg Met His Ala Vai Leu tcc atc ggt tcc Ser Ile Gly Ser atg gat gat cgc tac gcg ctg ctc aac Met Asp Asp Arg Tyr Ala Leu Leu Asn aca gtg Thr Vai atc gag gaa Ile Glu Giu aaa gat gag atc gtg gcg cag tct gcg Lys Asp Giu Ile Val Ala Gin Ser Ala atc tac tca Ile Tyr Ser aac gca gtg Asn Ala Val acc cca ccg tgg ggc att gag gat cag gat gaa ttc Thr Pro Pro Trp Gly Ile Giu Asp Gin Asp Giu Phe ctc gtt Leu Val gtt gag gtt gaa Val Giu Val Giu acc ccc atc gag Thr Pro Ile Giu ctg cgc cgt ggc Leu Arg Arg Gly C aa.

Gin aaa ctc gaa gaa.

Lys Leu Giu Giu gcc Ala 75 gcc gag cgg gtc Ala Giu Arg Val cgc gtc cgc aaa tgg ggg Arg Val Arg Lys Trp Gly 80 cca cgc acc ctc Pro Arg Thr Leu gtg gat atc gtg cag atc att aaa gat Val Asp Ile Val Gin Ile Ile Lys Asp ggg .gaa Gly Giu 100 gag atc ctt Glu Ile Leu tgg cag cgt Trp Gin Arg 120 tct Ser 105 gag gat ccc gaa Giu Asp Pro Giu acc ttg cca cac Thr Leu Pro His cct tgg gct Pro Trp Ala 115 gaa cct gat Giu Pro Asp 451 499 gcc ttc gtg ttg Ala Phe Val Leu atc Ile 125 cct tgg ttg gaa Pro Trp Leu Giu gcc gtc Ala Val 135 ctg cac ggc acg Leu His Giy Thr acc Thr 140 att gca gaa cat Ile Ala Glu His gat aat ctt gat Asp Asn Leu Asp ccc aca gac att gaa ggt gtc acc aag att Pro Thr Asp Ile Giu Gly Vai Thr Lys Ile taaggagtcg tggctttcat 1089 WO 01/00843 150 155 gca <210> 786 <211> 159 <212> PRT <213> Corynebacteriun glutamicum PCTItB0O/00923 600 <400> 786 Met His Ala Val Leu Ser Ile Gly Ser Asn Met Asp Asp Arg 1 Leu Ser Phe Leu Val Ile Pro Glu Val 145 Leu Ala Leu Leu Arg Lys His Ala 130 Asp Asn Ile Asn Arg Lys Asp Pro 115 Glu Asn Lys Giy Val Glu Asp Glu Phe Gly Asp Ile Glu Ala Val Asp Val1 Thr Glu Glu Glu Al a Asp Pro Leu Thr 140 Leu Asp Pro Thr Asp Ile Glu Gly Val Thr Lys Ile <210> 787 <211> 609 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (101)..(586) <223> RXN01304 <400> 787 atgcaggtaa acgaatttgt gcttatatca acattcgtga ttcggcaaaa ttaattaaac tgaaaaaggg gattaattac ccccacttga ggagaaattg atg ccc gca cag aac 115 Met Pro Ala Gin Asn 1 aaa aac ctc cca gga tcc gtc atc gtt gtg tct gat cgg att aaa tcg 163 Lys Asn Leu Pro Gly Ser Val Ilie Val Val Ser Asp Arg Ile Lys Ser 1090 WO 01/00843 gga Gly gaa Giu gaa Giu gtc Val c cg Pro acg Thr tc t Ser atc Ile gtg Val1 150 att Ile gtg Val cat His acc Thr aca Thr ttg Leu 105 ctg Leu gcg Ala gat Asp gag Giu caa Gin atc Ile gaa Giu ttc Phe gtg Val1 ccg Pro aaa Lys att Ilie gaa Giu ggc Gly ccg Pro tc t Ser ggc Giy agt Ser gca Ala tcC Ser t tg Leu gga Giy cac His gga Giy t tg Leu tct Ser 140 15 gta Vai ac c Thr gcg Ala gtg Val acg Thr 95 aat Asn cgc Arg ggc Gly gta Vai gtg Val gcg Ala cct Pro ctg Leu gcg Ala tcc Ser cgc Arg 145 PCT/IB00100923 ctt cag 211 Leu Gin gac Asp gag Giu cgc Arg aga Arg ccg Pro cag Gin acg Thr 130 gac Asp ctt Leu gag Giu cgg Arg aat Asn ggt Gly gcg Al a 115 gcc Ala gcg Ala ggc Gly gat Asp cgg Arg ctg Leu 100 ggg Gly gcg Ala ctc Leu ccg ctt ttc ggt tcc att ttt gag cgt ctt Pro Leu Phe Giy Ser Ile Phe Giu Arg Leu taaaagattt ttgcttatcg acg <210> 788 <211> 162 <212> PRT <213> Corynebacteriun glutamicun <400> 788 Met Pro Ala Gin Asn Lys Asn Leu Pro 1 5 Asp Arg Ile Lys Ser Gly Giu Arg Ilie 25 Vai Asp Leu Leu Gin Giu Ser Gly Vai 40 Vai Giu Giu Gly Phe Giu Pro Vai His 55 Ala Arg Arg Asp Arg Vai Ile Ile Thr 70 Ser Vai Ile Vai Vai Ser Lys Ala Gly Pro Val Ala Ile Ser Thr Phe Thr Val Giu Leu Vai Lys Ala Leu Gly Giy Thr Gly Val Gly 75 1091 WO 01/00843 WO 0100843PCT1IiBO00923 Pro Leu Ala Ser Arg 145 Arg Asfl Arg Thr Gly Leu Met 100 Ala Giy Leu 115 Ala Ala Leu Ala Leu Gly Giu Ala Thr Glu 90 Gin Ile Leu Phe 105 Arg Gly Leu Val 120 Val Asn Ala Pro 135 Val Cys Pro Leu His Ile Asp Thr Giy Leu Ser Asn 110 Leu Ser Ala Arg 125 Ser Ser Gly Gly 140 Gly Ser Ile Phe <210> 789 <211> 1281 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(1258) <223> RXS02556 <400> 789 tgccatcata ttaaggccaa attgcttgga tcctgggatt gtagaaatta gcccatgaag catggaaagg cgaaaacccc cag Gin atc Ile ccg Pro aac Asn ctg Leu gc t Ala ccc Pro aaa Lys gtt Val acc Thr gcg Ala cct Pro att Ile gca Ala t tc Phe ttc Phe gc t Ala gac Asp act Thr acg Thr tac Tyr aac Asn tcg Ser cc a Pro ga t Asp ctt Leu aac Asn cgt Arg att Ile gtt Val tcg Ser i5 Gly gct Ala caa Gin acc Thr cgc Arg 95 tatttaatta gattaaatcc ttg atc gtt tcc acc Leu Ile Val Ser Thr 1 gaa cac gca gag gtg Glu His Ala Giu Vai att aat gag atc acg Ile Asn Giu Ile Thr cca gaa ttg atc gct Pro Giu Leu Ile Ala gat cag cag aag gcg Asp Gin Gin Lys Ala ctc gtt act cct gat Leu Val Thr Pro Asp ggc cac aag cac gtg Gly His Lys His Val 100 1092 WO 01/00843 tcc ctc ggc att act gct gat cag tac gac att gtt cac PCT/IiBOO/00923 Ser Leu Gly Ilie Thr Ala Asp Gin 105 ttc gcc Phe Ala gtc gct Val Ala 135 atc ggt Ile Giy 150 gac gtc Asp Val acc gtc Thr Val ggt cag Gly Gin ctg cgc Leu Arg 215 gcg gtt Ala Val 230 tcc gtt Ser Vai ctt aac Leu Asn tcc acc Ser Thr gtt gtg 1027 Val Val 295 gag gaa 1075 Giu Giu 310 atc ttc 1123 Ile Phe gca Ala 120 gaa Giu tt t Phe Zttc Phe tgg Trp tac Tyr 200 cag Gin gag Glu ggt G ly aag Lys cca Pro 280 gt t Val1 gtg Val1 tac Tyr atc Ile gcC Ala gag Giu cgc Arg gaa Giu 185 acc Thr tac Tyr gat.

Asp gac Asp gac Asp 265 atg Met ttg Leu cag Gin cgc Arg gtt Val1 tgg Trp aac Asn gaa Giu 170 tac Tyr tcc Ser agc Ser aac Asn aag Lys 250 acc Thr gtg Val1 cat His ggg Gly gac Asp gag Giu gat Asp aac Asn 155 gtc Val1 acc Thr atc Ilie t tg Leu ggc Gly 235 att Ile aat Asn ggC Giy gct Aia ctc Leu 315 aac Asn gtt Val1 gc t Ala 140 ctt Leu acc Thr ctg Leu gga Gly ctc Leu 220 gag Giu gaa Glu cca Pro atg Met gac Asp 300 gtc Val gac Asp t tg Leu 125 g tc Val1 tat Tyr gtg Val1 gca Ala gta Val1 205 ggc Gly gt t Val1 gcc Ala gt t Val1 ctc Leu 285 cag Gin gaa Giu cag Gin Tyr 110 gga Giy tac Tyr gc t Ala acc Thr ggt Giy 190 gtg Val ggC Gly tc t Ser acc Thr gtg Vai 270 gca Ala gcc Ala aag Lys tgg Asp Ilie Val His gcg gaa Ala Giu tgg atc Trp Ile tcc aac Ser Asn 160 gcg aag Ala Lys 175 gag ctg Glu Leu ctt gac Leu Asp tcc gac Ser Asp gga ttc Gly Phe 240 atc gcg Ile Ala 255 ctg att Leu Ile ggt atg Giy Met gag tcc Giu Ser ctc cct Leu Pro 320 gtc Val1 130 gca Ala ctg Leu cag Gin gcc Ala ggc Gly 210 gag Glu cgt Arg 9gC Gly cag Gin cct Pro 290 tac Tyr gct Ala gag cac Giu His 115 acc gca Thr Ala aat gtg Asn Val gag cct Glu Pro ctc agc Leu Ser 180 cca gag Pro Giu 195 gcc cgc Ala Arg tac cgc Tyr Arg gat cgc Asp Arg gac ctg Asp Leu 260 ggc atc Gly Ile 275 gaa cgt Giu Arg gcg cag Ala Gin gcg ttt Ala Phe c tg Leu cct Pro ctg Leu ggc Gly 165 gca Ala cca Pro cag Gin at t Ile gta Val1 245 gt t Val1 Gly gac Asp gtg Val gaa Giu 325 ctc gag gtc gct ggc cgc att Leu Giu Val Ala Gly Arg Ile Trp 1093 WO 01/00843 330 cca tca ggt gcg tcc gtg tac ctg 1171 Pro Ser Giy Ala Ser Val Tyr Leu 345 aac gtg cgt gag cag atc gag gcg 1219 Asn Val Arg Giu Gin Ile Giu Ala 360 365 aac ttc gag ctc ttc gca cca aac 1268 Asn Phe Giu Leu Phe Ala Pro Asn 375 380 ccccagaact tcc 1281 <210> 790 <211> 386 <212> PRT <213> Corynebacteriun giutamicum <400> 790 tgc 350 ctc Leu gac Asp Leu Ile Val Ser Thr Gin 1 Giu Ile Pro Asp Leu Gly Val1 Thr Met 145 Asp Lys' Giu Ile Ile Lys Pro His 100 His Ala Val1 Pro Ser Ile Pro Asn Leu 70 Ala Ser Phe Val1 Ile 150 Asp Thr Pro Lys Val1 Thr Ala Pro Leu Ala Ala 135 G ly Val1 Val1 Ile Thr Ala Thr 25 Phe Tyr Phe Asn Ala Ser Asp Pro Gly Ilie 105 Ala Ile 120 Giu Ala Phe Giu Phe Arg Trp Giu ggC Gly gag Giu ctg Leu Arg Pro Lys Gly Ala Gin Ala Giu Asp Asn 155 Val Thr Ser Leu Met Asn Thr Leu Asp Val1 Ala 140 Leu Thr Leu Ala Val Phe Gin Phe Leu Gin Leu 125 Val Tyr Val1 Ala PCTTBOO/00923 340 gtg gaa ttc ttg aag Val Giu Phe Leu Lys 355 cag cct cgc gac gta Gin Pro Arg Asp Val 370 att tcc taagcccaca Ile Ser 385 Leu Gly Ala Lys Ala Ser Tyr 110 Gly Tyr Ala Thr Gly Ser Gly Ala Gin.

Thr Arg Asp Ala Trp Ser Ala 175 Glu 1094 WO 01/00843 WO 0100843PCTIBOOIOO923 180 185 190 Vai Ala Pro Giu Pro Giy Gin Tyr Thr Ser Ile Gly Val Val Leu Asp 195 200 205 Asp Gly Ala Arg Gin Leu Arg Gin Tyr Ser Leu Leu Giy Gly Ser Asp 210 215 220 Thr Giu Tyr Arg Ile Ala Val Giu Asp Asn Gly Giu Val Ser Gly Phe 225 230 235 240 Leu Arg Asp Arg Vai Ser Val Gly Asp Lys Ilie Giu Aia Thr Ile Ala 245 250 255 Ala Gly Asp Leu Val Leu Asn Lys Asp Thr Asn Pro Val Val Leu Ile 260 265 270 Ser Gin Gly Ilie Giy Ser Thr Pro Met Val Giy Met Leu Ala Giy Met 275 280 285 Asn Pro Giu Arg Asp Val Val Val Leu His Ala Asp Gin Ala Giu Ser 290 295 300 Thr Tyr Ala Gin Val Giu Giu Val Gin Gly Leu Val Giu Lys Leu Pro 305 310 315 320 Lys Ala Ala Phe Giu Ile Phe Tyr Arg Asp Asn Asp Gin Trp Leu Giu 325 330 335 Val Ala Gly Arg Ile Pro Ser Giy Ala Ser Val Tyr Leu Cys Gly Gly 340 345 350 Val Giu Phe Leu Lys Asn Vai Arg Giu Gin Ile Giu Ala Leu Asp Giu 355 360 365 Gin Pro Arg Asp Val Asn Phe Giu Leu Phe Ala Pro Asn Asp Trp Leu 370 375 380 Ile Ser 385 <210> 791 <211> 990 <212> DNA <213> Corynebacterium giutamicum <2 <221> CDS <222> (101)..(967) <223> RXS02560 <400> 791 ttggggcaag ccagctaacg catttcttgt ggaaaccgca gacattgagg ccgcccacgc ggaacttcta agagcagtgg aatgaaataa tccggtgctg atg cag ggc aac tcg 115 Met Gin Gly Asn Ser 1 ctt aat ctg gca gac aac agc gag aga aag aag ccc atg ccg tca cca 163 Leu Asn Leu Ala Asp Asn Ser Glu Arg Lys Lys Pro Met Pro Ser Pro 1095 WO 01/00843 WO 0100843PCTIBOOIOO923 gga gaa ctt Gly Giu Leu ccg cag tgg Pro Gin Trp, gcc gcc cgc tac Ala Ala Arg Tyr gga Gly caa cct gca acc Gin Pro Ala Thr tgg acg cca Trp, Thr Pro cga tca gtt Arg Ser Val aat gag acg ctt Asn Giu Thr Leu gtc att cac cag Val Ile His Gin cgc agg Arg Arg tgg ttg gat aaa Trp Leu Asp Lys gtt gat gat gac acc atc cgc acc att Val Asp Asp Asp Thr Ile Arg Thr Ile tcc gcc gca caa Ser Ala Ala Gin gct gga acc tct Ala Gly Thr Ser aat aag cag gtc Asn Lys Gin Val 211 259 307 355 403 451 499 tct gtc atc gtg Ser Val Ilie Val aaa gat cct gag Lys Asp Pro Giu agg aaa ggc ctc Arg Lys Gly Leu gcg ggg Ala Gly 100 atc act cgc Ile Thr Arg att tgg ttg Ilie Trp Leu 120 cag Gin 105 atg ttt ccg cac Met Phe Pro His ctt Leu 110 gag cag gtt ccc gcg gtg ctg Glu Gin Val Pro Ala Val Leu att gat tat tcc Ile Asp Tyr Ser atc agt gcg gtg gca gcc aga gaa Ile Ser Ala Val Ala Ala Arg Giu 130 gat ctc Asp Leu 135 cca aca ggg gct ctt gat tat ctc gat Pro Thr Gly Ala Leu Asp Tyr Leu Asp 140 gag Giu 145 gcc gcg tgg ggg Ala Ala Trp Gly ctc gac gcc gga Leu Asp Ala Gly gca gct caa aac Ala Ala Gin Asn gc t Ala 160 gca att gct gcg Ala Ilie Ala Ala tca ctt gga ttg Ser Leu Gly Leu acg ctc tat ttg Thr Leu Tyr Leu tcg gtg cgc aac Ser Val Arg Asn gat gcg Asp Ala 180 gaa gcc gtg Giu Ala Val gtg ggc ttg Val Gly Leu 200 cac His 185 aaa ttg ctt ggc Lys Leu Leu Gly cca cct gag atc Pro Pro Glu Ile gtg cct gtc Val Pro Val 195 gaa atg ggg cat Giu Met Gly His gcg gat ccg cct gaa cct gcc gga att Ala Asp Pro Pro Giu Pro Ala Gly Ile 205 210 aaa cct Lys Pro 215 ccc ctg cca caa Pro Leu Pro Gin gcc att gtt cac Ala Ile Val His tgg Trp 225 gat acc tac acc Asp Thr Tyr Thr aaa aac ctc gaa Lys Asn Leu Giu ctt Leu 235 atc gat tcc tac Ilie Asp Ser Tyr cgc gcc ctc gac Arg Ala Leu Asp act Thr 245 tac tat tct cgc Tyr Tyr Ser Arg tac Tyr 250 ggc cag cac cag Gly Gin His Gin tgg tcg aag cag Trp Ser Lys Gin acg gcg Thr Ala 260 1096 WO 01/00843 WO 0100843PCTJIBOO/00923 cat agg gcg gcg tcg aaa agc ttt tca aaa acc aac agg cag ttc ctt His Arg Ala Ala Ser Lys Ser Phe Ser Lys Thr Asn Arg Gin Phe Leu 265 270 275 agg ggc gtg ttt gag cgc gcc ggg ttt ggg ctg aga taaaagcatg Arg Gly Val Phe Giu Arg Ala Gly Phe Gly Leu Arg 280 285 attatggacg cct <210> 792 <211> 289 <212> PRT <213> Corynebacteriun giutamicun <400> 792 Met Gin Gly Asn Ser Leu Asn Leu Ala 1 Pro Ala Gin Thr Asn Lys Val1 Val Giu 145 Ala Val1 Giu Glu Met Thr His Ile Lys Gly Pro Ala 130 Ala Ile Arg Ile Pro 210 5 Pro Pro Val Ile Ile G ly Leu Giu Gly Giu 165 Ala Val Ile Gly Pro Arg Ile 70 Ser Ile Ile Asp Phe 150 Ser Giu Val Lys Giu Gin Arg Ser Val1 Thr Trp Leu 135 Leu Leu.

Ala Gly Pro 215 Leu Trp Trp Ala Ile Arg Leu 120 Pro Asp Gly Val Leu 200 Pro Leu Asn Leu Ala Val Gin 105 Ile Thr Ala Leu His 185 Giu Leu Asp 10 Ala Giu Asp Gin Val 90 Met Asp Gly Gly Gly 170 Lys Met Pro Asn Ala Thr Lys Ser 75 Lys Phe Tyr Ala Ile 155 Thr Leu Giy Gin Arg Gly Val1 Asp Thr Giu Leu 110 Ile Tyr Gin Leu Leu 190 Asp Ile Lys Gin Ile Asp Ser Leu G iu Ser Leu Asn Gly 175 Pro Pro Val1 Lys Pro His Asp Ser Arg Gin Ala Asp Ala 160 Ser Pro Pro His Trp Asp Thr Tyr Thr Giu Lys Asn Leu Giu Leu Ile Asp Ser Tyr Asp 1097 WO 01/00843 PCTJIBOO/00923 225 230 235 240 Arg Ala Leu Asp Thr Tyr Tyr Ser Arg Tyr Gly Gin His Gin Leu Trp, 245 250 255 Ser Lys Gin Thr Ala His Arg Ala Ala Ser Lys Ser Phe Ser Lys Thr 260 265 270 Asn Arg Gin Phe Leu Arg Gly Val Phe Giu Arg Ala Gly Phe Gly Leu 275 280 285 Arg <210> 793 <211> 1425 <212> DNA <213> Corynebacteriun glutanicun <220> <221> CDS <222> (101)..(1402) <223> RXA00382 <400> 793 aaaacactat tgaccacccc aatcgctgtt gagagtttgt aaagttcgac tgtcaacgag ctggattggc ttcacagatt gaattaatac aatgacgcac atg aca tcg tcc aat Met Thr Ser Ser Asn gag Glu cct Pro aaa Lys gac Asp gca Ala ggc Gly cgc Arg acc c tc Leu ggc Gly gat Asp atg Met gcc Ala gag Glu ctg Leu 115 cgc acc Thr gga Gly gtg Val ctg Leu gtc Val1 ttg Leu 100 gtc Val1 ggt 1098 WO 01/00843 Ser Gly Thr Glu Ala Thr Met Ser Ala Val Arg Leu Ala Arg PCT112BOO/00923 120 125 130 act cag Thr Gin 135 gtc gat Vai Asp 150 ctg cct Leu Pro gtt gtt Vai Vai tac cca Tyr Pro atg ggc Met Gly 215 atc gct Ile Ala 230 ggc ttc Gly Phe gct gac Ala Asp gca gcg Ala Ala ggc ccc 1027 Giy Pro 295 gca gct 1075 Ala Ala 310 acc atc 1123 Thr Ile tta acc 1171 Leu Thr att Ile gca Ala 155 ggc Giy gac Asp gcc Ala cca Pro ggc Gly 235 tac Tyr ttc Phe aag Lys gca Ala tcg Ser 315 gc a Ala gta Val1 t tg Leu 140 tc t Ser atc Ile att Ile tgc Cys aag Lys 220 gcg Ala cgt Arg ggc Gly gc t Ala ggc Giy 300 ctt Leu gat Asp gcc Ala tac Tyr gca Ala tc t Ser gc t Ala gcc Ala 210 aag Lys gaa Giu gac Asp ggc Gly ctg Leu 290 ccg Pro tcc Ser atc Ile c ac His act Thr gac Asp ttt Phe 195 gg t Gly ctt Leu gtc Val aag Lye cta Leu 275 gcc Ala gtt Val1 ctc Leu tct Ser Gly ggc Gly ttc Phe act Thr 180 gcg Al a ggc Gly C tC Leu atg Met gtt Val 260 cct Pro cca Pro gcg Ala tac Tyr gat Asp 340 547 595 643 691 739 787 835 883 931 979 cag cgt gcc tca aac atg Gin Arq Ala Ser Asn Met 1099 WO 01/00843 WO 0100843PCT/EBOO/00923 345 -350 ctg tct atc cgt ttt gca gaa ggt gag 1219 Leu Ser Ilie Arg Phe Ala Glu Gly Giu 360 365 aag gca gcc gac atc ttc cgc ttc gca 1267 Lys Ala Ala Asp Ile Phe Arg Phe Ala 375 380 gac aac ggc gtc tac gca cca cca agc 1315 Asp Asn Gly Val Tyr Ala Pro Pro Ser 390 395 tct tcc gct ctc acg gac gat gat ttc 1363 Ser Ser Ala Leu Thr Asp Asp Asp Phe 410 aag ccc gcc gca cgt gca gca gca gaa 1412 Lys Pro Ala Ala Arg Ala Ala Ala Glu 425 430 cattgtccat cta 1425 <210> 794 <211> 434 <212> PRT <213> Corynebacteriun giutamicum <400> 794 Met Thr Ser Ser Asn Thr Ala Arg Ser 1 Gin Lys Leu Thr Pro Gly Gly Val Asn Ser Val Gly Gly Gin Ala Arg Phe Ile Leu Ile Asp Val Asp Gly Asn Glu Tyr 55 Gly Pro Met Leu Met Gly His Ala His 70 Gin Lys Ala Val Val Asp Gly Leu Ser Giu Val Giu Leu Ala Gin Asp Ile Val 100 105 Val Arg Leu Val Asn Ser Gly Thr Giu 115 120 ca c His ttc Phe t tc Phe 400 aag Lys aag Lys Glu Pro Lys Asp Al a 75 Gly Arg Thr aac Asn ttc Phe 385 gaa G iu atc Ile gca Ala Trp, Val Ala Leu Val1 Ala Thr Met 355 tct gat atg Ser Asp Met act ttg ctg Thr Leu Leu tgg ttt gtg Trp Phe Val 405 cag gca ctc Gin Ala Leu 420 tgacgcaaac Al a Gly Thr Trp Val Gly Giu Arg 1100 WO 01/00843 Leu Ala Arg 130 Cys Tyr His 145 Val Ala Thr Thr Ser Asp Asn Ala Phe 195 Ala Ala Gly 210 Asp Lys Leu 225 Asp Glu Val Val Asp Lys Gly Gly Leu 275 Met Leu Ala 290 Asn Pro Val 305 Giu Ser Leu Leu Ile Ser Arg Ala Ser 355 Asn Phe Ser 370 Phe His Thr 385 Giu Thr Tip Thr Val1 150 Leu Val1 Tyr Met Ile 230 Gly Ala Ala Gly Ala 310 Thr Leu Leu Lys Asp 390 Ser Glri 135 Asp Pro Val Pro Gly 215 Ala Phe Asp Al a Pro 295 Ala Ile Thi- Ser Ala 375 Asn Ser Arg Ala Asp Pro Gly 200 Thr His Arg Leu Phe 280 Val Gly Asn His Ile 360 Ala Gly Ala Ser Leu Ser Tyr 185 Glu Val Ala Thr Val1 265 Gly Tyr Arg Ala Glu 345 Arg Asp Val Leu Lys Leu Pro 170 Asn I le Ala Asp Ser 250 Thr Gly Gin Ala ASn 330 Gly Phe Ile Tyr Thr 410 Ile Ala 155 Gly Asp Ala Pro Gly 235 Tyr Phe Lys Ala Ser 315 Ala Val1 Ala Phe Ala 395 Asp Leu 140 Ser Ile Ile Cys Lys 220 Ala Arg Gly Ala Gly 300 Leu Asp Ala Glu Arg 380 Pro Asp Phe Gly Gly Ala 190 Ile Asn Leu Ti-p Val1 270 Ile Leu Leu Leu His 350 Glu Ala Ser Phe PCTJIBOO/00923 Glu Gly Ser Gly 160 Ala Gln 175 Val Arg Ala Glu Phe Asn Ile Leu 240 Phe Gly 255 Val Ser Met Asn Ser Gly Ala Asp 320 His Gly 335 Ile Gin Gly His Pro Phe Val Phe 400 Ser Lys 415 Ile Glu Gln Ala Leu Lys Pro Ala Ala Arg Ala Ala Ala Glu Ala Lys Ala Ser 420 430 1101 WO 01/00843 WO 0100843PCTTIBOOIOO923 <210> 795 <211> 1233 <212> DNA <213> Corynebacteriun <220> <221> CDS <222> (101)..(1210) <223> RXA00156 <400> 795 accgagagcg tggtacgcct glutamicun catttzagttt cctcctatga atcttgatgt ggttcatgcg tttttatgca atatcaacca aaagttggta cgatcctcat atg aat gaa cgc aca Met Asn Giu Arg Thr tcg gat gca ttt gac gcc ctc ctt gtg ctc tcc ttc ggt ggt Ser Asp Ala Phe Asp Ala Leu Leu Val Leu Ser Phe Giy Gly ccc gaa Pro Giu ggg cac gag Gly His Giu gtt cgt ccg ttt Val Arg Pro Phe gag aat gtc act Giu Asn Val Thr cac gga agg His Gly Arg ggg att ccg ccg gaa cgt cta Gly Ile Pro Pro Giu Arg Leu ttc ggt ggt atc agc ccc atc Phe Gly Gly Ilie Ser Pro Ile gat Asp 45 gaa gtg gcg gtt cat tac cac cac Giu Val Ala Val His Tyr His His aat gcg ctg aac agg gaa Asn Ala Leu Asn Arg Giu att atc gcc :Ie Ile Ala aa t Asn gtg gaa aaa gaa Val Giu Lys Giu gcg tct cgc gat Ala Ser Arg Asp aag ctg cct gtt Lys Leu Pro Vai ttt ggt aac cgc Phe Gly Asn Arg tgg aag ccg ttt gat aat gag gcc gct Trp Lys Pro Phe Asp Asn Glu Ala Ala gaa caa Glu Gin 100 atg gct gat Met Ala Asp tgg ggt ggc Trp Giy Gly 120 ggc gtg aaa aac Gly Vai Lys Asn ctg gtg ttg gca Leu Val Leu Ala act tcc gct Thr Ser Ala 115 tac tcc ggt tgt Tyr Ser Giy Cys cgg Arg 125 cag tac cag gaa gat att cag ggc Gin Tyr Gin Giu Asp Ile Gin Gly 130 atg atc Met Ile 135 aag cac ctg gag Lys His Leu Giu cag ggg cag tcg atc acg ttc acc aag Gin Gly Gin Ser Ile Thr Phe Thr Lys c tg Leu 150 cgt cag ttc tac Arg Gin Phe Tyr cac cct cgt ttt gtc tcc acc atg gct His Pro Arg Phe Vai Ser Thr met Ala 160 caa Gin 165 ttg gtt cag gat Leu Val Gin Asp tac gcg aag ctt Tyr Ala LYS Leu gat gag ctg cga Asp Giu Leu Arg gat gag Asp Giu 180 1102 WO 01/00843 gcg cgt ctg gtc ttc acc gcg cac tcc Ala Arg Leu Val Phe Thr Ala His Ser 185 190 gct gcg gga acc cct gag gat ggc tcc Ala Ala Gly Thr Pro Glu Asp Gly Ser 200 205 gaa gcg tca gca ctg att gct gag gct Glu Ala Ser Ala Leu Ile Ala Glu Ala 215 220 gtg gtg tgg cag tcc cgc tcg ggt agc Val Val Trp Gin Ser Arg Ser Gly Ser 230 235 cct gac atc gtg gat cac gca gtg gag Pro Asp Ile Val Asp His Ala Val Glu 250 gcg ctc gtt gtc tgc cct gta ggc ttt Ala Leu Val Val Cys Pro Val Gly Phe 265 270 att tgg gat ctt gat tcc gag ctg atg Ile Trp, Asp Leu Asp Ser Giu Leu Met 280 285 atg gtg gtc gag cgt gtc gct acc gtt 1027 Met Val Val Giu Arg Val Ala Thr Val 295 300 gcc ctt gtg gtt gat ctc atc gag gag 1075 Ala Leu Val Val Asp Leu Ile Giu Glu 310 315 gag cgc ctt gga aag ctg cca gca cgc 1123 Glu Arg Leu Gly Lys Leu Pro Ala Arg 330 ccg tgt ggc gac ggc tgc tgt ggt acc 1171 Pro Cys Gly Asp Gly Cys Cys Gly Thr 345 350 gtg aac ccc aac gct cgc tca gcg gcg 1220 Val Asn Pro Asn Ala Arg Ser Ala Ala 360 365 agtccctcgc aaa 1233 <210> 796 <211> 370 <212> PRT <213> Corynebacteriun glutamicum att Ile t tg Leu gt t Val1 ccg Pro ctc Leu 255 att Ile gaa Glu ggc Gly gca Ala gga Gly 335 gcc Ala cca Pro cca Pro tat Tyr ggt Gly c ac His 240 aac Asn tct Ser gaa Glu ccc Pro gag Giu 320 ag t Ser aag Lys gct Ala ctg Leu tcc Ser gtg Val1 225 act Thr gag Giu gat Asp gcc Ala ac c Thr 305 C tc Leu tcc Ser cat His gcc Al a PCTIBOOIOO923 gcg gac aat 691 Ala Asp Asn 195 cag gtc aag 739 Gin Val Lys gat ttt gat 787 Asp Phe Asp tgg ctg gag 835 Trp Leu Glu 245 ggt caa aaa 883 Gly Gin Lys 260 atg gaa gtc 931 Met Giu Val 275 aag cgc aac 979 Lys Arg Asn gaa ttc gca Glu Phe Ala cgc gtt atc Arg Val Ile 325 aac ggc gca Asn Gly Ala 340 acc gcg cgg Thr Ala Arg 355 taggagtgat 1103 WO 01/00843 PCT/IB00/00923 <400> 796 Met Asn Glu Arg Thr Ser Asp Ala Phe Asp Ala Leu Leu Val Leu Ser 1 5 10 Phe Gly Gly Pro Glu Gly His Glu Glu Val Arg Pro Phe Leu Glu Asn 25 Val Thr His Gly Arg Gly Ile Pro Pro Glu Arg Leu Asp Glu Val Ala 40 Val His Tyr His His Phe Gly Gly Ile Ser Pro Ile Asn Ala Leu Asn 55 Arg Glu Ile Ile Ala Asn Val Glu Lys Glu Leu Ala Ser Arg Asp His 70 75 Lys Leu Pro Val Tyr Phe Gly Asn Arg Asn Trp Lys Pro Phe Asp Asn 90 Glu Ala Ala Glu Gin Met Ala Asp Asp Gly Val Lys Asn Ala Leu Val 100 105 110 Leu Ala Thr Ser Ala Trp Gly Gly Tyr Ser Gly Cys Arg Gin Tyr Gin 115 120 125 Glu Asp Ile Gin Gly Met Ile Lys His Leu Glu Ser Gin Gly Gin Ser 130 135 140 Ile Thr Phe Thr Lys Leu Arg Gin Phe Tyr Asp His Pro Arg Phe Val 145 150 155 160 Ser Thr Met Ala Gin Leu Val Gin Asp Ser Tyr Ala Lys Leu Pro Asp 165 170 175 Glu Leu Arg Asp Glu Ala Arg Leu Val Phe Thr Ala His Ser Ile Pro 180 185 190 Leu Thr Ala Asp Asn Ala Ala Gly Thr Pro Glu Asp Gly Ser Leu Tyr 195 200 205 Ser Thr Gin Val Lys Glu Ala Ser Ala Leu Ile Ala Glu Ala Val Gly 210 215 220 Val Ser Asp Phe Asp Val Val Trp Gin Ser Arg Ser Gly Ser Pro His 225 230 235 240 Thr Pro Trp Leu Glu Pro Asp Ile Val Asp His Ala Val Glu Leu Asn 245 250 255 Glu Lys Gly Gin Lys Ala Leu Val Val Cys Pro Val Gly Phe Ile Ser 260 265 270 Asp His Met Glu Val Ile Trp Asp Leu Asp Ser Glu Leu Met Glu Glu 275 280 285 Ala Glu Lys Arg Asn Met Val Val Glu Arg Val Ala Thr Val Gly Pro 290 295 300 Thr Asp Glu Phe Ala Ala Leu Val Val Asp Leu Ile Glu Glu Ala Glu 305 310 315 320 1104 WO 01/00843 WO 0100843PCT/BOOIOO923 Leu Lys Arg Val Ile Glu Arg Leu Gly Lys Leu Pro Ala Arg Gly Ser 325 330 335 Ser Val Asn Gly Ala Pro Cys Gly Asp Gly Cys Cys Gly Thr Ala Lys 340 345 350 His Lys Thr Ala Arg Val Asn Pro Asn Ala Arg Ser Ala Ala Pro Ala 355 360 365 Ala Asn 370 <210> 797 <211> 810 <212> DNA <213> Corynebacterium giutamicum <2 <221> CDS <222> (101)..(787) <223> RXA00624 <400> 797 tccatgacgt tttgaatgga aaatctccat ttgtggagtt agaagaagac cactagtttt caacaggacg acaacggccg gacatgcgac aatacaatgc atg tcc ggc cgt ctt 11! Met Ser Gly Arg Leu ctt Leu agg Arg gtc Val1 ac c Thr ctc Leu aac Asn caa Gin tgg Trp gtt Val C tc Leu gca Ala c tc Leu aac Asn ctt Leu atg Met aga Arg tca Ser at t Ile ccc Pro gcg Ala ggt Gly gaa Giu cag Gin atg Met agt Ser ctg Leu gat Asp gaa Glu caa Gin 75 gaa Glu ggc Gly ggc Gly acc Thr atc Ile ctc Leu cgc Arg cgt Arg ctt Leu atc Ile ctc Leu 1 cga Arg gag Glu gcc Ala ggc Gly cgc Arg acc Thr ttt Phe cca Pro 1105 WO 01/00843 WO 0100843PCTIBOOIOO923 120 125 aac gtc gcC gcc tcg acc agg gga Asn Val Ala Ala Ser Thr Arg Gly I 135 140 ttc ttg gcg tgt aga aac ctc tcc Phe Leu Ala Cys Arg Asn Leu Ser 150 155 aaa tgg tgg cgc aag aat gtc atc Lys Trp Trp Arg Lys Asn Vai IleI 170 aaa gga aat aca gtg cgc ttg tcc Lys Gly Asn Thr Val Arg Leu Ser 185 cct aaa gtc gca gct gac ttc cgg Pro Lys Val Ala Ala Asp Phe Arg 200 205 ttg ggt gct cag gtg caa acc tatt Leu Gly Ala Gin Val Gin Thr Tyr 215 220 tagttgggga ggttcggggc acc <210> 798 <211> 229 <212> PRT <213> Corynebacterium giutamicum <400> 798 Met Ser Gly Arg Leu Leu Val Ser 1 5 Arg Ser Ala Ala Asp Arg Leu Ile Giu Val Ser Leu Leu Val Ala Pro 3 Ala Lys Asp Lys Gly Thr Leu Ala 'I Gly His Giu Leu Ile Leu Asn Gly 1 70 Arg Ser Giu Phe Ala Asn Leu Glu Thr Gly Ala Ile Arg Gin Met Gin I 1001 Phe Ala Pro Pro Arg Ti-p Arg Met 1 115 120 Pro Glu Phe Asp Phe Asn Val Ala 130 135 :ta cat aac .eu His Asn ;tg lal iag .sys Ica ;aa flu :ct er fl er ~rg ~rp 'he ~rg ~ys ;er ggt Gly gct Ala 175 tcg Ser gct Ala cag Gin Ser Asp Ile Met Asp His Ile Glu Ser gaa G lu 160 gtc Val gcg Ala gca Ala gcg Ala Ser Leu Asp Giu Gin 75 Giu Gly Gly Thr ggc Gly gct Ala gcg Al a 180 acc Thr ttg Leu ctg Leu gaa Glu gca Ala 165 gaa Glu aac Asn gat Asp gcc Ala Thr Ile Leu Arg Arg Leu Ile Leu Asn 1106 WO 01/00843 WO 0100843PCTIEBOO/00923 Leu Asp Thr Gly Glu Phe Leu Ala Cys Arg Asn 145 150 155 Gly Phe Gly Ala Ala Lys Trp Trp Arg Lys Asn 165 170 Thr Arg Gly Ala Glu Lys Gly Asn Thr Val Arg 180 185 Arg Asn Leu Thr Asn Pro Lys Val Ala Ala Asp 195 200 Leu Ala Ala Leu Asp Leu Gly Ala Gln Val Gln 210 215 Ala Ala Gln Leu Ala 225 <210> 799 <211> 956 <212> DNA <213> Corynebacteriun glutamicum Leu Ser Val Val Ile Lys Leu Ser Ala 190 Phe Arg Glu 205 Thr Tyr Ser 220 <220> <221> CDS <222> <223> RXA00306 <400> 799 ccc Pro acc Thr gaa Glu acc Thr acc Thr cag Gln gt t Val ctt Leu acg cag ttg gtg gag ggc agc tgg ttt gaa ccg Thr Gln Leu Val Glu Gly Ser Trp Phe Glu Pro 10 ttt Phe att Ile gcg Ala gc t Ala cag Gin att Ile agt Ser ccg Pro ggc Gly gcg cat ctc Ala His Leu cat His gat Asp gcg Ala 110 agc Ser tcc Ser aac Asn cag Gln ccc Pro 1107 WO 01/00843 WO 0100843PCT/BOOIOO923 125 gca gca Ala Ala 130 gca cgc acc acc gcg tgg ctt aac cac Ala Arg Thr Thr Ala Ti-p Leu Asn His 135 ttt Phe 140 gaa aaa gcc aag Glu Lys Ala Lys caa ggc gtt ggt Gin Gly Val Gly ggt ttc atc gcc Gly Phe Ilie Ala caa cgt ctg gag Gln Arg Leu Giu gaa Giu 160 gac gag gcg gat Asp Glu Ala Asp gag Glu 165 aaa tcc gat atc Lys Ser Asp Ile gct gaa tcc atg Ala Glu Ser Met acc cag Thr Gin 175 480 528 576 624 tac ttc gag Tyr Phe Giu acc gca tgg Thr Ala Trp, 195 gat Asp 180 cct ctc ggc cct Pro Leu Gly Pro gaa Glu 185 att gag gag tac Ile Giu Glu Tyr ttc acc cgc Phe Thr Arg 190 agc tcc cgc Ser Ser Arg ctt cgt gaa caa Leu Arg Glu Gin act Thr 200 cgc gat tcc att Arg Asp Ser Ile ctg Leu 205 ttc aaa Phe Lys 210 gtt cgc cct ggc Val Arg Pro Gly gcc cgg gaa caa Ala Arg Giu Gin atc agc ctg gcc gat Ile Ser Leu Ala Asp 220 ttg agg ctc acc cgc Leu Arg Leu Thr Arg gcg Al a 225 gaa gaa ggc atg Giu Giu Gly Met ttt agt cct gtc Phe Ser Pro Val acc gat ggt cct Thr Asp Gly Pro cgt Arg 245 tgg tcc cat gat T-p, Ser His Asp gat gag cat gtg Asp Giu His Val gct tcc Ala Ser 255 atc gtc gca Ilie Val Ala gaa atg tac Giu Met Tyr 275 gga Gly 260 ctt aac cca cat Leu Asn Pro His gga Gly 265 ctc ccc ttt gaa Leu Pro Phe Giu gaa atc ctg Glu Ile Leu 270 ctg cac aac Leu His Asn gcg atg gct caa Ala Met Ala Gin ggt G ly 280 atc gag gga gaa Ile Giu Gly Glu tcc Ser 285 ggc gcc Gly Ala 290 att gcg gcg ttg Ile Ala Ala Leu gat ctc atc cgc Asp Leu Ile Arg c ac His 300 gga ttg gtg ttg Gly Leu Val Leu ccc Pro 305 gct gat ctt ctc Ala Asp Leu Leu gat tct ASP Ser 310 taaataagga ctgattgtga aag <210> 800 <211> 311 <212> PRT <213> Corynebacterium glutamicun <400> 800 Asp Ser Gly Ile Pro Thr Gin Leu Val Giu Gly Ser Ti-p Phe Glu Pro 1 5 10 Val Arg Gly Arg Thi- Phe Asp Arg Ile Ile Ala Asn Pro Pro Phe Val 1108 WO 01/00843 WO 0100843PCTIiBOO/00923 25 Val Giy Pro Pro Giu Ile Gly His Val Tyr Arg Asp Ser Gly Met Asp 40 Leu Asp Gly Ala Thr Ala Leu Val Val Lys Giu Ala Cys Ala His Leu 55 Asn Pro Gly Gly Thr Ala His Leu Leu Gly Ala Trp Val His Ser Ala 70 75 Asp Gln Ser Trp Gin Gin Arg Val Ala Glu Trp Leu Pro Asp Asn Gly 90 Tyr Val Ala Trp Val Ilie Giu Arg Asp Ala Val Ser Pro Ala Gin Tyr 100 105 110 Val Giy Thr Trp Leu Ser Asp Glu Ser Leu Asp Leu Arg Ser Pro Giu 115 120 125 Ala Ala Ala Arg Thr Thr Ala Trp Leu Asn His Phe Giu Lys Ala Lys 130 135 140 Val Gin Giy Vai Giy Phe Gly Phe Ile Ala Ile Gin Arg Leu Giu Giu 145 150 155 160 Asp Giu Ala Asp Glu Lys Ser Asp Ilie Leu Ala Giu Ser Met Thr Gin 165 170 175 Tyr Phe Glu Asp Pro Leu dly Pro Giu Ilie Giu Giu Tyr Phe Thr Arg 180 185 190 Thr Ala Trp, Leu Arg Giu Gin Thr Arg Asp Ser Ilie Leu Ser Ser Arg 195 200 205 Phe Lys Vai Arg Pro Giy Val Ala Arg Giu Gin Ile Ser Leu Ala Asp 210 215 220 Ala Giu Giu Gly Met Gly Phe Ser Pro Val Thr Leu Arg Leu Thr Arg 225 230 235 240 Thr Asp Gly Pro Arg Trp Ser His Asp Val Asp Giu His Val Ala Ser 245 250 255 Ile Val Ala Gly Leu Asn Pro His Gly Leu Pro Phe Giu Giu Ile Leu 260 265 270 Giu Met Tyr Ala Met Ala Gin Gly Ile Giu Gly Giu Ser Leu His Asn 275 280 285 Gly Ala Ile Ala Ala Leu Val Asp Leu Ile Arg His Gly Leu Val Leu 290 295 300 Pro Ala Asp Leu Leu Asp Ser 305 310 <210> 801 <211> 1263 <212> DNA <213> Corynebacterium giutamicun 1109 WO 01/00843 WO 0100843PCT/iBOO/00923 <220> <221> CDS <222> (101)..(1240) <223> RXA00884 <400> 801 catcttccgt ttcataccct gcactctacc ctgttcttag gaattcgcta tgtttaacat caattaatca tgtatagggg gcaggcacta ggcttggggc atg tca gtt ttt Met Ser Val Phe gtg Val t tc Phe acc Thr ctg Leu ccg Pro cgc Arg aa t Asn ggc Gly ttg Leu gcc Al a 150 at t Ile aat Asn att Ile acc Thr ctt Leu aat Asn ttg Leu act Thr gag Glu aac Asn 120 gtg Val gag Glu ggc G ly gtg Val cat His tat Tyr gac Asp cct Pro att Ile ttt Phe tct Ser 105 agg Arg ctg Leu gca Ala acg Thr ctc Leu 185 gtg Val act Thr t cg Ser c99 Arg ggt Gly 9gc Gly ac c Thr att Ile gac Asp cgt Arg c cg Pro 170 gaa G lu c cg Pro gc t Ala ttg Leu cag Gin gcg Ala 75 att Ile tcg Ser tcg Ser cgc Arg gag Giu 155 aca Thr gcg Ala ttt Phe 9gg Gly gaa Glu gcg Ala gac Asp gcg Ala cct Pro tta Leu acg Thr 140 gcg Ala gag Glu aac Asn tgt Cys gaa Glu gtt Val1 gaa Giu ggt Gly gat Asp gag Glu ggg Gly 125 cac His ggg Gly acc Thr gtg Val tca Ser t ta Leu 30 gag Glu act Thr ttg Leu ggt Gly ttt Phe 110 atg Met acc Thr ttt Phe gat Asp gat Asp 190 act Thr 15 ggt Gly ttg Leu atc Ile gcc Al a gcg Ala 95 ttt Phe cag Gin cca Pro gag Glu ga t Asp 175 cac His cgg Arg agt Ser gag Giu ttt Phe agg Arg 80 gaa Glu gat Asp tcg S er ggg Gly cat His 160 gat Asp gtg Val tat Tyr ggc Gly gtg Val ggg Gly ggg Gly acg Thr cgt Arg 115 tca Ser gtg Val t tg Leu aag Lys tat Tyr 195 tgc Cys ccg Pro gct Ala ggt Gly gc t Ala gag Glu 100 gag Glu agc Ser gcg Ala gac Asp acg Thr 180 tcc Ser ggt Gly gat Asp gac Asp tcg Ser acc Thr gtg Val tcc Ser gcg Ala gt t Val gcg Ala atg Met 165 ctg Leu ttg Leu 115 163 211 259 307 355 403 451 499 547 595 643 691 1110 WO 01/00843 WO 0100843PCT/LBOO/00923 atc gtg aaa gat ggc acg gcg atg gcg cgc aag gtg cac aag ggc gag Ile Val Lys 200 Asp Gly Thr Ala Met Ala Arg Lys Val His Lys Gly Giu 205 210 ctg cca gcg ccg gac gag gat gtct Leu Pro Ala Pro Asp Glu Asp Val) 215 220 gac gct cgc ctg cgc tca gct ggtt Asp Ala Arg Leu Arg Ser Ala Gly 230 235 tgg gcg aaa ccc ggc gga gaa tgc i Trp Ala Lys Pro Gly Gly Glu Cys I 250 gac ggc gac tgg tgg ggc gcg ggc Asp Gly Asp Trp Trp Gly Ala Gly 265 gac cgc cgc ttc tac aac atc aagc Asp Arg Arg Phe Tyr Asn Ile Lys 1 280 285 att gcg gcc ggc gag ctg ccc att z 1027 Ilie Ala Ala Gly Giu Leu Pro Ile 295 300 gaa gat cac cac acc gag cgc gtc 1075 Glu Asp His His Thr Glu Arg Val 310 315 ggc gtg ccg ctg aac ctt ttc gca 1123 Gly Val Pro Leu Asn Leu Phe Ala 330 cgt cat atc gca ggg ggc ctg ctgc 1171 Arg His Ile Ala Gly Gly Leu Leu 345 gcg gtg acc gat gcg gga cgt ttg 1219 Ala Val Thr Asp Ala Gly Arg LeuI 360 365 att ttg ctt agt gaa gaa gac taaat 1263 Ile Leu Leu Ser Glu Glu Asp 375 380 <210> 802 <211> 380 <212> PRT <213> Corynebacteriuni glutamicun <400> 802 ac Eyr :tc ?he ~ag .ys :cg ?ro ~ac ~is ~ag Itg let :cc ro :ac :tt atc Ile aac Asn 245 gtc Val1 Gly cag Gin gcg Ala .caa Gin 325 gac Asp ctg Leu gac ,eu Ala Asp Gly Ilie Ile Ala Asp 370 ;attta gtagggttac aga 1111 WO 01/00843 Met Ser Va 1 Cys Gly Ty Thr Ala Gi 3 Met Ala Va Ie Gly Gi Val Leu GI Val Thr Th Gly Leu Ax 11 Ala Ser Se 130 Arg Pro Va 145 Val Asn Le Val Arg Ly Ser Ala Ty 19 Val His Ly 210 Arg Phe GI 225 Tyr Glu Va Met Gly Ty His Ser Hi 27 Arg Tyr Se 290 Giu Arg Le 305 Leu Arg Le His Tyr Asp Pro Ile Phe Ser 105 Arg Leu Ala Thr Leu 185 Asp Pro Leu Pro Trp 265 Phe Gly His Leu Val1 10 Thr Ser Arg Gly Gly Thr Ile Asp Arg Pro 170 Giu Gly Asp Arg Gly 250 Trp Tyr Glu Thr Asn Phe Gly Glu Ala Asp Ala Pro Leu Thr 140 Ala Glu Asn Ala Asp 220 Ala Glu Ala Ile Pro 300 Arg Phe Cys Glu Val1 Glu Gly Asp Giu Gly 125 His Gly Thr Val Met 205 Val Gly Cys Gly Lys 285 Ile Val1 Ala PCTABOO/00923 Thr Arg Gly Ser Leu Glu Ile Phe Ala Arg s0 Ala Glu Phe Asp Gin Ser Pro Gly Glu His 160 Asp Asp 175 His Val Arg Lys Ala Asp Asp Trp 240 His Asn 255 Gly Ala Pro Ala Giu Thr Leu Gly 320 Ala Ala WO 01/00843 WO 0100843PCTIIBOO/00923 325 Arg Pro Val Ile Asp Arg His Ile Ala 340 345 335 Gly Gly Leu Leu His Val Asn 350 Leu Ala Asp Ala Leu Gly Asn Leu Ala Val Thr Asp Ala Gly Arg Leu 355 360 365 Gly Ilie Ilie Ala Asp Ile Leu Leu Ser Giu Giu Asp 370 375 380 <210> 803 <211> 522 <212> DNA <213> Corynebacteriun g lutamicum <220> <221> <222> <223>

CDS

(101)..(499) RXN0 2503 <400> 803 gcagcaccgg caaccacgtc cgtcaacgcg tcagaactgc cggatgcggg tatcgtcgca ttcgtgaacg caccttctgc cacacaaacg agggagtaac atg acc tta aaa Met Thr Leu Lys 1 ggt acc cga gga Gly Thr Arg Gly aaa ctt gcc acc Lys Leu Ala Thr caa gct ggc acc Gin Ala Gly Thr atc cgc Ile Arg gac cag ctg Asp Gin Leu acc cct ggt Thr Pro Gly aaa Lys cac tac gga cgc His Tyr Gly Arg gct gaa ctg cac Ala Giu Leu His atc gtg acc Ile Val Thr ggc gtc ggc Gly Val Gly gat gtc aac atg Asp Val Asn Met tcc Ser cca gtc gag cgt Pro Val Giu Arg gtg ttc Val1 Phe acc cag gcg ctg Thr Gin Ala Leu gat gtg ttg cat Asp Val Leu His ggt gaa tgc gat Gly Giu Cys Asp gct gtg cac tcc Ala Val His Ser aag gat ctg ccg Lys Asp Leu Pro gcc acc gat cct Ala Thr Asp Pro ttc cac ctg gtc Phe His Leu Val cca act cgt gcg Pro Thr Arg Ala tcg cgc gag gcc Ser Arg Glu Ala ctt atc Leu Ile 100 gcc cgc gac Ala Arg Asp ggg aac ttc Gly Asn Phe 120 ggc Gly 105 ctg act ctg gct Leu Thr Leu Ala gag Giu 110 ctt cca gaa agg Leu Pro Giu Arg cgc aaa ggt Arg Lys Gly 115 aat ccg ccc Asn Pro Pro cgc tcc tcg acg Arg Ser Ser Thr cat His 125 ctc cca gct caa- Leu Pro Ala Gin ggc Gly 130 1113 WO 01/00843 PCTILBOO/00923 tgacctggag attctcccac tgc 522 <210> 804 <211> 133 <212> PRT <213> Corynebacterium giutanicun <400> 804 Met Thr Leu Lys Ile Gly Thr Arg Gly Ser Lys Leu Ala Thr Thr Gin 1 5 10 Ala Gly Thr Ile Arg Asp Gin Leu Lys His Tyr Giy Arg Asp Ala Giu 25 Leu His Ilie Val Thr Thr Pro Gly Asp Vai Asn Met Ser Pro Val Giu 40 Arg Ile Gly Val Gly Val Phe Thr Gin Ala Leu Arg Asp Val Leu His 55 Ser Gly Giu Cys Asp Val Ala Val His Ser Met Lys Asp Leu Pro Thr 70 75 Ala Thr Asp Pro Arg Phe His Leu Val Val Pro Thr Arg Ala Asp Ser 90 Arg Giu Ala Leu Ile Ala Arg Asp Gly Leu Thr Leu Ala Giu Leu Pro 100 105 110 Giu Arg Arg Lys Gly Gly Asn Phe Arg Ser Ser Thr His Leu Pro Ala 115 120 125 Gin Giy Asn Pro Pro 130 <210> 805 <211> 558 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (101)..(535) <223> FRXA02503 <400> 805 gcagcaccgg caaccacgtc cgtcaacgcg tcagaactgc cggatgcggg tatcgtcgca ttcgtgaacg caccttctgc cacacaaacg agggagtaac atg acc tta aaa att 115 Met Thr Leu Lys Ile 1 ggt acc cga gga tcc aaa ctt gcc acc acc caa gct ggc acc atc cgc 163 Gly Thr Arg Gly Ser Lys Leu Ala Thr Thr Gin Ala Gly Thr Ile Arg 15 gac cag ctg aaa cac tac gga cgc gac gct gaa ctg cac atc gtg acc 211 Asp Gin Leu Lys His Tyr Giy Arg Asp Ala Giu Leu His Ile Vai Thr 30 1114 WO 01/00843 WO 0100843PCTIBOO/00923 acc cct ggt gat gtc aac atg tcc c Thr Pro Giy Asp Val Asn Met Ser 1 45 gtg ttc acc cag gcg ctg cgc gat c Val Phe Thr Gin Ala Leu Arg Asp N gtg gct gtg cac tcc atg aag gat c Val Ala Val His Ser Met Lys Asp 1 75 ttc cac ctg gtc gtg cca act cgt Phe His Leu Val Val Pro Thr Arg 1 cgc ccn cga cgg sct gan ttt kggt Arg Pro Arg Arg Xaa Xaa Phe Xaa 105 tgg gaa ctt tcc gct cct cga cgc i Trp Giu Leu Ser Ala Pro Arg Arg 120 125 cct gac ctg gag att ctc cca ctt c Pro Asp Leu Giu Ile Leu Pro Leu 2 135 140 atgggcaagg tca <210> 806 <211> 145 <212> PRT <213> Cory-nebacteriun giutaxnicum <400> 806 Met Thr Leu Lys Ile Gly Thr Arg 1 Ala Gly Thr Ilie Arg Asp Gin Leu 1 Leu His Ile Val Thr Thr Pro Gly 1 Arg Ilie Gly Val Gly Val Phe Thr( Ser Gly Giu Cys Asp Val Ala Val 1 Ala Thr Asp Pro Arg Phe His Leu 1 Arg Arg Xaa Pro Tyr Arg Pro Arg I 100 Gin Xaa Xaa Xaa Arg Trp Giu Leu 115 120 atc ggc gtc Ile Giy Val ggt gaa tgc Gly Giu Cys acc gat cct Thr Asp Pro cga ggs cct Arg Xaa Pro 100 aar gsg saa.

Xaa Xaa Xaa 115 aag gca atc Lys Ala Ile 130 tgacaccggc ggC Gly gat Asp cga Arg tat Tyr agg Arg cgc Arg 259 307 355 403 451 499 Thr Asp Pro Vali Leu Ala Xaa 110 Ile 1115 WO 01/00843 WO 0100843PCTIiBOO/00923 Leu Lys Ala Ile Arg Pro Asp Leu Glu Ile Leu Pro Leu Ala Arg Lys 130 135 140 His 145 <210> 807 <211> 1245 <212> DNA <213> Corynebacterium giutamicun <220> <221> <222> <223>

CDS

(101)..(1222) R.XA0 0377 <400> 807 aatggcgctc gggcagggcg cgcaagtact aaccagcaat tcccaagccc aaaaacccct cccatatagt tctttttact gatgcattgt cctcaattag gtg tgg ctt ctt ttc Val Trp Leu Leu Phe cta aat tgg gat Leu Asn Trp Asp tgg ggc aag att Trp Gly Lys Ile gag Giu cgc atg tct gct Arg Met Ser Ala ctt act Leu Thr att cca gct Ile Pro Ala gct aat ggc Ala Asn Gly cgt cgc acg cta Arg Arg Thr Leu aac gcg ccc att Asn Ala Pro Ile att gat gcc Ile Asp Ala atg cgc cag Met Arg Gin aag acc ccg act Lys Thr Pro Thr act ccg gtg tgg Thr Pro Vai Trp gcg ggt Ala Giy agg tcg ttg cct Arg Ser Leu Pro tac aag aag gtc Tyr Lys Lys Val gag gga atc agc Glu Giy Ile Ser atg Met ttg gat tcc tgt Leu Asp Ser Cys atg ccg gag ttg Met Pro Giu Leu ttg Leu gcg gag att act Ala Giu Ile Thr cag ccg gtt cgt Gin Pro Val Arg cat gat gtg gat His Asp Val Asp gcg att ttg ttc Ala Ile Leu Phe tct gac Ser Asp 100 att gtg gtg ccg ttg cgt gct gcg Ile Val Val Pro Leu Arg Ala Ala 105 gtt ggt gtg gaa atc gtg gcg Val Gly Val Giu Ile Val Ala 115 ggt cgt gga Gly Arg Gly 120 cct gtg ttg gat Pro Val Leu Asp ccg gtg cgg agc Pro Val Arg Ser cgt Arg 130 ggg gat gtg Giy Asp Val ttg aat Leu Asn 135 ctt cct att ttg gag ggc aac gtt ccg Leu Pro Ile Leu Glu Gly Asn Val Pro 140 gag Glu 145 gtg gag cag ggt Val Glu Gin Gly 1116 WO 01/00843 att ggc atc Ile Gly Ile 150 ttt gct ggt Phe Ala Gly cct tcc aag Pro Ser Lys gag acg tgg Glu Thr Trp 200 tct ttg aag Ser Leu Lys 215 gat tcg tgg Asp Ser Trp 230 ttg ccg tat Leu Pro Tyr cct cgt att Pro Arg Ile agc aag gct Ser Lys Ala 280 gat aag gct 1027 Asp Lys Ala 295 cag ggt aac 1075 Gin Gly Asn 310 act aag gaa 1123 Thr Lys Glu gca ggt cat 1171 Ala Gly His cct aat acg 1219 Pro Asn Thr 360 ttg Leu ccg Pro 170 cat His gcg Ala cag Gin ggg Gly act Thr 250 ttt Phe tca Ser gag Glu gat Asp gag Glu 330 acg Thr gcg Ala gat Asp 155 ttt Phe gag Giu ttg Leu atc Ile ttc Phe 235 gag Glu gg t Gly gag Giu cgt Arg cc t Pro 315 cgc Arg ggc Gly gaa Glu ttg Leu ttg Leu acc Thr gct Ala 205 gcg Ala act Thr ttg Leu ggt Gly atg Met 285 gc t Ala t tg Leu aag Lys atc Ile at t Ile 365 gat Asp agt Ser 175 gca Ala ttg Leu atc Ile cgt Arg gaa Glu 255 ggt Gly gtg Val1 gta Vai ttt Phe gag Glu 335 aac Asn gaa G lu tct Ser 160 tac Tyr atg Met gtg Val gat Asp gat Asp 240 gtg Vai gag Giu gat Asp tca Ser gcg Ala 320 gc t Aia ctt Leu gcc Ala cag Gin ttg Leu atg Met ccg Pro gcg Ala 225 tac Tyr 9gc Gly t tg Leu tgg Trp ggt Giy 305 ggt Gly cag Gin gg t Giy gtc Val1 gcg Ala gt t Val cat His acg Thr 210 gtg Val1 acc Thr aag Lys ctt Leu cgg Arg 290 cc t Pro cgc Arg act Thr cat His tcc Ser 370 t tg Leu gag Giu gg t Giy 195 att Ile cag Gin gag Giu tac Tyr gg t Gly 275 gtg Val1 aag Lys gca Al a gct Ala ggt Gly 355 atc Ile PCTIBOOIOO923 att ggt 595 Ile Gly .165 ggt ggt 643 Gly Gly 180 gat cct 691 Asp Pro gtg aat 739 Val Asn ttg ttt 787 Leu Phe ttc gtg 835 Phe Val 245 cag ctg 883 Gin Leu 260 gcg atg 931 Ala Met ccg ttg 979 Pro Leu gtg ttg Val Leu cct ttg Pro Leu 325 gtt gat Val Asp 340 gtg ctt Val Leu att cat Ile His 1117 WO 01/00843 PCTJIBOO/00923 tct taaactaaga ggagtttcat gcg 1245 Ser <210> 808 <211> 374 <212> PRT <213> Corynebacterium giutamicun <400> 808 Val Tip, Leu Leu Phe Leu Asn Tip Asp Lys Tip Gly Lys Ile Giu Arg 1 5 10 Met Ser Ala Leu Thr Ile Pro Ala Ala Arg Arg Thr Leu Asn Asn Ala 25 Pro Ile Ile Asp Ala Ala Asn Gly Lys Thr Pro Thr Arg Thr Pro-Val 40 Trp Phe Met Arg Gin Ala Gly Arg Ser Leu Pro Giu Tyr Lys Lys Val 55 Arg Glu Gly Ile Ser Met Leu Asp Ser Cys Phe Met Pro Giu Leu Leu 70 75 Ala Giu Ilie Thr Leu Gin Pro Val Arg Arg His Asp Val Asp Ala Ala 90 Ilie Leu Phe Ser Asp Ile Val Val Pro Leu Arg Ala Ala Gly Val Gly 100 105 110 Val Giu Ile Val Ala Gly Arg Gly Pro Val Leu Asp Ala Pro Val Arg 115 120 125 Ser Arg Gly Asp Val Leu Asn Leu Pro Ile Leu Giu Giy Asn Val Pro 130 135 140 Giu Val Glu Gin Giy Ile Giy Ilie Ile Leu Asp Giu Leu Ser Asp Ser 145 150 155 160 Gin Ala Leu Ile Gly Phe Aia Gly Ala Pro Phe Thr Leu Ala Ser Tyr 165 170 175 Leu Val Giu Gly Gly Pro Ser Lys Asn His Giu Lys Thr Lys Ala Met 180 185 190 Met His Gly Asp Pro Giu Thr Trp His Aia Leu Met Ala Arg Leu Val 195 200 205 Pro Thr Ilie Val Asn Ser Leu Lys Ser Gin Ile Asp Ala Gly Ile Asp 210 215 220 Ala Val Gin Leu Phe Asp Ser Trp Ala Gly Phe Leu Thr Giu Arg Asp 225 230 235 240 Tyr Thr Giu Phe Val Leu Pro Tyr Ser Thr Giu Ile Leu Giu Glu Val 245 250 255 Gly Lys Tyr Gin Leu Pro Arg Ilie His Phe Gly Val Gly Thr Gly Glu 1118 WO 01/00843 WO 0100843PCT/EBOO/00923 260 265 Leu Leu Gly Ala Met Ser LyS Ala Gly Ser Giu 275 280 Trp Arg Val Pro Leu Asp Lys Ala Ala Glu Arg 290 295 Gly Pro Lys Val Leu Gin Gly Asn Leu Asp Pro 305 310 315 Gly Arg Ala Pro Leu Thr Lys Giu Ile Glu Arg 325 330 Gin Thr Ala Val Asp Ala Gly His Ala Thr Gly 340 345 Gly His Gly Val Leu Pro Asn Thr Val Ala Giu 355 360 Val Ser Ile Ile His Ser 370 <210> 809 <211> 681 <212> DNA !<213> Coryriebacteriun glutamicum <2 <221> CDS <222> (101)..(658) <223> R.XN02504 <400> 809 cctcgattcc acctggtcgt gccaactcgt gcggactcgc gacggcctga ctctggctga gcttccagaa aggcgcaaag Met 285 Ala Leu Lys Ile Ile 365 270 Gly Ala Leu Ala Phe 350 Thr Asp Ser Ala 320 Ala Leu Ala cc t Pro ctc Leu ggt Gly ggc Gly ccc Pro atc Ile cgc Arg gat Asp gac Asp gca Ala tcc Ser gga Gly gc t Al a cgc Arg cag Gin gcgaggccct tatcgcccgc gtg gga act tcc gct Val Gly Thr Ser Ala 1 cct gac ctg gag att Pro Asp Leu Giu Ile ggc aag gtc acc tcc Gly Lys Val Thr Ser ggc ctc acc cgc gtc Gly Leu Thr Arg Val gcc gac atc atc atg Ala Asp Ile Ile Met tgc cgc gcc gac gac Cys Arg Ala Asp Asp 1119 WO 01/008 act gaa Thr Glu gtt Val tgt Cys gac Asp cag Gin 150 ggc G ly c tc Leu 43 acc gtc Thr Val gcg gtt Ala Val 105 gcg cct Ala Pro 120 atg act Met Thr gta ttc Val Phe ctc gtt Leu Val gac cgc Asp Arg 185 cgc Arg gca Ala gtc Val1 ctc Leu tcc Ser gca Ala 170 agc Ser gcg ctc aac atg ctg atg cac Ala Leu Asn Met Leu Met His 95 gaa cgc acc gtg ctc aac cgc Glu Arg Thr Val Leu Asn Arg 110 gca gcg cac gcc acc ttg gac Ala Ala His Ala Thr Leu Asp 125 acc gcc ggc gtc tac gca ctt Thr Ala Gly Val Tyr Ala Leu 140 145 gcc gaa ggt gac ggc gcc cgc Ala Glu Gly Asp Gly Ala Arg 155 160 caa cag ctt atc gac gcc gga Gin Gin Leu Ile Asp Ala Gly 175 taattagggc ccgaaatttc cat gcc Ala c tc Leu ggc Gly 130 gac Asp cca Pro gac Asp gaa Glu 115 tac Tyr ggc Gly gaa Glu PCT/iBOO/00923 acg ttt 403 Thr Phe 100 gct ggc 451 Ala Gly tcc ggc 499 Ser Gly tct gac 547 Ser Asp gag ctc 595 Glu Leu 165 aat ttg 643 Asn Leu 180 gcc gcc Ala Ala <210> 810 <211> 186 <212> PRT <213> Corynebacterium glutamicum <400> 810 Val Gly Thr Ser Ala Pro Arg Arg Ile 1 Pro Asp Leu Glu Ilie Leu Pro Leu Arg Gly Lys Val Thr Ser Gly Glu Leu Asp 40 Gly Leu Thr Arg Val Gly Met Gin Asp 55 Ala Asp Ile Ile Met Pro Ala Pro Ala 70 Cys Arg Ala Asp Asp Thr Glu Thr Val His Ala Asp Thr Phe Val Ser Ala Val 100 105 Arg Leu Giu Ala Gly Cys Thr Ala Pro 115 120 Asp Gly Tyr Ser Gly Asp Thr Met Thr 130 135 Gin Asn Val1 Ala Gly Ala Giu Ala Thr Leu Ile Met Thr Ala Leu Arg Ala Ala 140 Lys Asp Leu Glu Leu Asn Thr His 125 G ly 1120 WO 01/00843 WO 0100843PCT/EBOO/00923 Leu Asp Gly Ser Asp Gin Leu Val Phe Ser Ala Glu Gly Asp Gly Ala 145 150 155 160 Arg Pro Giu Giu Leu Gly Glu Leu Val Ala Gin Gin Leu Ile Asp Ala 165 170 175 Gly Ala Ala Asn Leu Leu Gly Asp Arg Ser 180 185 <210> 811 <211> 561 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS <222> (101)..(538) <223> FRXA02504 <400> 811 gcatctccca gctcaaggca atccgccctg acctggagat tctcccactt gcgcggaaac attgacaccg gcatgggcaa ggtcacctcc ggtgaactcg atg ctt gtg atg ctc Met Leu Val Met Leu gcc Ala gtt Val1 gcg Ala atg Met gtg Val gcC Ala gtc Val gac Asp atc tac Tyr ttc Phe atc Ile ctg Leu ctc Leu ac c Thr tac Tyr ggC Gly gac gca Ala gac Asp gaa Giu atg Met aac Asn ttg Leu gca Ala gcc Ala 120 gcc ggc Gly gcc Ala tgc Cys cac His cgc Arg gac Asp ctt Leu 105 cgc Arg gga ctc Leu gac Asp cgc Arg gcc Ala c tc Leu ggC Gly gac Asp cca Pro gcC acc Thr atc Ile gcc Ala gac Asp gaa Giu 75 tac Tyr ggc Gly gaa Giu gcc cgc Arg atc Ile gac Asp acg Thr 60 gct Ala tcc Ser tct Ser gag Giu aat gtc Val1 atg Met gac Asp 45 tt t Phe ggc Gly ggc Gly gac Asp C tc Leu 125 ttg ggc Gly ccc Pro 30 act Thr gtt Val tgt Cys gac Asp cag Gin 110 ggc Gly c tc atg Met 15 gcc Ala gaa Glu tcc Ser acc Thr ac c Thr 95 c tg Leu gag Glu 99C 1 cag gac cgc Gin Asp Arg ccc gca cag Pro Ala Gin acc gtc cgc Thr Val Arg 50 gcg gtt gca Ala Val Ala 65 gcg cct gtc Ala Pro Val 80 atg act ctc Met Thr Leu gta ttc tCC Val Phe Ser ctc gtt gca Leu Val Ala 130 gca Ala ggc Gly 35 gcg Ala gaa Giu gca Ala acc Thr gcc Ala 115 c aa Gin acg Thr 20 gca Ala ctc Leu cgc Arg gcg Ala gcc Ala 100 gaa Giu cag Gin gaa Glu ctt Leu aac Asn acc Thr cac His ggC Gly gg t Gly ctt Leu 115 163 211 259 307 355 403 451 499 548 gac cgc agc taattagggc 1121 WO 01/00843 PCTIBOO/00923 Ile Asp Ala Gly Ala Ala Asn Leu Leu Gly Asp Arg Ser 135 140 145 ccgaaatttc cat 561 <210> 812 <211> 146 <212> PRT <213> Corynebacterium glutamicun <400> 812 Met Leu Val Met Leu Ala Tyr Ala Gly Leu Thr Arg Val Gly Met Gin 1 5 10 Asp Arg Ala Thr Glu Val Phe Asp Ala Asp Ile Ile Met Pro Ala Pro 25 Ala Gin Gly Ala Leu Ala Ile Glu Cys Arg Ala Asp Asp Thr Giu Thr 40 Val Arg Ala Leu Asn Met Leu Met His Ala Asp Thr Phe Val Ser Ala 55 Val Ala Giu Arg Thr Val Leu Asn Arg Leu Glu Ala Gly Cys Thr Ala 70 75 Pro Val Ala Ala His Ala Thr Leu Asp Gly Tyr Ser Gly Asp Thr Met 90 Thr Leu Thr Ala Gly Val Tyr Ala Leu Asp Gly Ser Asp Gln Leu Val 100 105 110 Phe Ser Ala Glu Gly Asp Gly Ala Arg Pro Glu Glu Leu Gly Giu Leu 115 120 125 Val Ala Gin Gin Leu Ilie Asp Ala Gly Ala Ala Asn Leu Leu Gly Asp 130 135 140 Arg Ser 145 <210> 813 <211> 1449 <212> DNA <213> Corynebacteriun glutanicun <2 <221> CDS <222> (101)..(1426) <223> RXN01162 <400> 813 catcgaatac gtgccctgct gaatagatga catcgcagag atctataaga gtcagtggtt gtcggggttt cacagtcact tattctatgc aggattcacc atg tat atc gtg ggg 115 Met Tyr Ile Val Gly 1 att tgt cta caa tta gtg gtt atg agc caa ccg atg tca gca ccc gac 163 1122 WO 01/00843 Ile Cys Leu Gin Leu Val Val Met Ser Gin Pro Met Ser Ala 15 tcc Ser gt c Val gca Ala C tc Leu gga Giy cgt Arg ttt Phe cca Pro gat Asp 150 gcc Ala caa Gin t tc Phe t tg Leu gat Asp 230 cca Pro gct Ala ggt Giy gaa Glu gga Gly tca Ser cgc Arg aaa Lys ctg Leu 135 aga Arg cgc Arg gaa Glu ctc Leu t tg Leu 215 ttg Leu cc a Pro cca Pro gac Asp t ca Ser c tc Leu tgg Trp cca Pro gag Glu 120 ttt Phe ctc Leu ttg Leu ccc Pro gtc Val 200 aa t Asn ggc Gly gc t Ala aca Thr cag Gin act Thr gca Ala cag Gin ccg Pro c tc Leu ggc Gly gt t Val tgg Trp 170 gag Giu ggt Gly ctc Leu act Thr gtg Val1 gag Giu gat Asp ttg Leu cag Gin 75 ctc Leu ggt G ly ggt G ly gtg Val att Ile 155 aca Thr aca Thr aaa Lys gga Gly gat Asp 235 tct Ser cgc Arg cca Pro att Ie caa Gin aat Asn aat Asn cgg Arg ggc Gly 140 ccg Pro gtc Val1 ctc Leu gat Asp ctg Leu 220 gag Giu gtt Val ggt Gly gca Ala ggt Gly gca Ala ctc Leu acc Thr cat His 125 ac c Thr gga Gly aac Asn atc Ile gaa Giu 205 ggg Gly gag Giu c tc Laul cat His cag Gin at t Ilie tta Leu gta Val aag Lys 110 gtt Val gca Ala cca Pro cgc Arg ccg Pro 190 ttc Phe gag Glu a tc Ile aac Asn gaa Giu gca Ala ggt Gly caa Giln cc t Pro 95 cat His gct Aia atg Met tca Ser aca Thr 175 att Ile agt Ser act Thr acc Thr gtg Val ttt Phe aga Arg ttt Phe gtg Val aag Lys gat Asp 115 tct S er c tg Leu ctt Leu tac Tyr ggc Gly 195 gtt Val1 gtg Val gct Ala gga Giy PCTIiBOO/00923 Pro Asp gcg gta 211 Ala Vai gcg cca 259 Ala Pro gag ggg 307 Giu Giy gtg att 355 Val Ile gca gag 403 Ala Glu 100 gcc ttg 451 Ala Leu ggc gat 499 Gly Asp ggg atg 547 Gly Met gct tgc 595 Ala Cys 165 cta gga 643 Leu Gly 180 gct caa 691 Ala Gin gcc acg 739 Ala Thr ctc agc 787 Leu Ser tca cat 835 Ser His 245 gct cgc 883 Ala Ara 1123 WO 01/00843 WO 0100843PCT/IBOO/00923 260 gaa gac Giu Asp acc gca atg Thr Ala Met ctt cgg gca Leu Arg Ala 280 ccc cac ttt Pro His Phe gac gta tca Asp Val Ser aac Asn 275 acc gtg gca Thr Val Ala gct Ala 285 gaa ccc acc Glu Pro Thr cag Gin 290 gga caa atg 979 Gly Gin Met ctg tgg 1027 Leu Trp 295 cgc gca 1075 Arg Ala 310 gag caa 1123 Giu Gin gtg aaa 1171 Val1 Lys caa gga 1219 Gin Gly cta ggc 1267 Leu Gly 375 gga gga 1315 Gly Gly 390 ctt cac 1363 Leu His atc gac 1411 Ile Asp cca gaa 1449 Pro Glu acc ttc ggg gat att Thr Phe Gly Asp Ile 300 gcc ggc aac aag gcg Ala Gly Asn Lys Ala 315 agc caa agg aat gct Ser Gin Arg Asn Ala 330 gag acc ctg tca ccc Giu Thr Leu Ser Pro gga gca gca ctt gcc tgc gat tgg cta Gly Ala Ala Leu Ala Cys Asp Trp Leu 305 cac gcc att agt ttt gcc tcc atg gtt His Ala Ile Ser Phe Ala Ser Met Val cgcaaa cta Arg Lys Leu 335 320 ggt gta tcc Gly Val Ser acc ctc agt Thr Leu Ser 340 aaa acg LYS Thr ctc Leu aaa gat atc cgc tat gta Lys Asp Ile Arg Tyr Vai 355 345 cca gaa Pro Giu 360 atc gat Ile Asp 350 tca gca agc cca cat Ser Ala Ser Pro His 365 cta gtt cct gaa acc Leu Val Pro Giu Thr gcc atc ttc atg aac aaa ggc Ala Ile Phe Met Asn Lys Gly 370 gca tgg atg atg ctg cgg cct Ala Trp Met Met Leu Arg Pro 380 aag ctc att gcg caa Lys Leu Ile Ala Gin gcc Ala 385 tcc aca gaa gac Ser Thr Giu Asp 400 aac atc gca aag Asn Ile Ala Lys 405 aca ctc caa Thr Leu Gin 410 gac aca gac Asp Thr Asp 425 gcg gtg aat Ala Val Asn 440 gaa caa cac ggc gga Giu Gin His Gly Gly 415 gtg cac caa tgg cga Val His Gin Trp Arg 430 atc atc aaa cac atc cgc Ile Ile Lys His Ile Arg 420 gtt aca aag ccg gtg act Vai Thr Lys Pro Val Thr tagcatcaaa aaccaacccc atg 1124 WO 01/00843 <210> 814 <211> 442 <212> PRT <213> Corynebacteriun giutanicui <400> 814 Met Tyr Ile Val Gly Ile Cys Leu Gin PCTIIBOOIOO923 1 Met Thr Ala Asp Ala Ala Asp Leu His 145 Ala Val Giu Ala Leu 225 Gly Ala Val Pro Ala Ala Glu Val Al a 100 Ala Gly Gly Ala Leu 180 Ala Ala Leu Ser Ala 260 Glu 5 Asp Val Pro Gly Ile Glu Leu Asp Met Cys 165 Gly Gin Thr Ser His 245 Arg Asp 5 er Val Al a Leu 70 Gly Arg Phe Pro Asp 150 Ala Gin Phe Leu Asp 230 Pro Thr Leu Ala Gly Glu Gly Ser Arg Lys Leu 135 Arg Arg Giu Leu Leu 215 Leu Pro Ala Arg Leu 10 Thr Gin Thr Ala Gin 90 Pro Leu Gly Val Trp 170 Glu Gly Leu Thr Val 250 Lys Thr Val Giu Asp Leu Gin Leu Gly Gly Val Ile 155 Thr Thr Lys Gly Asp 235 Ser Pro Val1 Val1 Arg Pro Ile Gin Asn Asn Arg Gly 140 Pro Val Leu Asp Leu 220 Glu Val His Ala met Ser Gly His Ala Gin Gly Ile Ala Leu Leu Val Thr Lys 110 His Val 125 Thr Ala Gly Pro Asn Arg Ile Pro 190 Giu Phe 205 Gly Glu Giu Ile Leu Asn Phe Giu 270 Ala Leu 285 Gin Glu Ala Gly Gin Pro His Ala Met Ser Thr 175 Ile Ser Thr Thr Vai 255 Gly Glu Thr Gin Gly Gin Met Leu Trp Thr Phe Gly Asp Ile Giy Ala Ala Leu 1125 WO 01/00843 290 Ala Cys Asp 305 Phe Ala Ser Vai Ser Thr Asp Ile Arg 355 Phe Met Asn 370 Met Met Leu 385 Asp Asn Ilie Ile Lys His Thr Lys Pro 435 PCT/EB00100923 295 Ala Gin Lys Gly Giy 375 Gly His Asp Glu Ala Ser Giu Pro 360 Ilie Lys Thr Asp Ala 440 Gly Gin Thr 345 Giu Asp Leu Leu Thr 425 Val Aia Lys Thr 350 His Thr Ser Giy Trp 430 Ile Leu 335 Leu Ala Ala Thr Gly 415 Arg Ser 320 Giy Lys Ile Trp Glu 400 Ile Val1 <210> <211> 1345 <2i2> DNA <213> Corynebacteriun giutaxnicum <22 0> <221> CDS <222> (i0i)..(1345) <223> FRXA01162 <400> catcgaatac gtgccctgct gaatagatga catcgcagag gtcggggttt cacagtcact tattctatgc aggattcacc at t Ilie tcc Ser gtC Val gca Ala gtg Val gag Giu ga t Asp ttg Leu atctataaga gtcagtggtt atg tat atc gtg ggg 115 Met Tyr Ile Val Giy 1 atg tca gca ccc gac 163 Met Ser Ala Pro Asp acc cat ttt gcg gta 211 Thr His Phe Ala Vai gct cct aga gcg cca 259 Ala Pro Arg Ala Pro gat ggg ttt gag ggg 307 Asp Gly Phe Glu Giy att ggt acc Ile Giy Thr WO 01/00843 PCT/IBO0100923 60 ctc gga ctc aag gca cag caa gca tta caa cgt gcc tct gtg gtg att 355 Leu Gly Leu Lys Ala Gin Gin Ala Leu Gin Arg Ala Ser Val Val Ile 75 80 gga tca tgg cgc cag ctc aat ctc gta cct gat gcc att aag gca gag 403 Gly Ser Trp Arg Gin Leu Asn Leu Val Pro Asp Ala Ile Lys Ala Giu 95 100 cgt cgc cca tgg ccg ggt aat acc aag cat cct gat tta gat gcc ttg 451 Arg Arg Pro Trp Pro Giy Asn Thr Lys His Pro Asp Leu Asp Ala Leu 105 110 115 ttt aaa gag ttc ctc ggt cgg cat gtt gct gtt ctg gcc tct ggc gat 499 Phe Lys Giu Phe Leu Giy Arg His Val Ala Val Leu Ala Ser Gly Asp 120 125 130 cca ctg ttt tac ggc gtg ggc acc gca atg gtc cat gtg ctg ggg atg 547 Pro Leu Phe Tyr Gly Val Gly Thr Ala Met Val His Val Leu Gly Met 135 140 145 gat aga ctc acg gtt att ccg gga cca tca tcc gcg tcg ctt gct tgc 595 Asp Arg Leu Thr Val Ile Pro Gly Pro Ser Ser Ala Ser Leu Ala Cys 150 155 160 165 gcc cgc ttg ggt tgg aca gtc aac cgc aca cgg gtg gtg tac cta gga 643 Ala Arg Leu Gly Trp Thr Val Asn Arg Thr Arg Val Val Tyr Leu Gly 170 175 180 caa gaa ccc att gag aca ctc atc ccg att att gaa tca ggc gct caa 691 Gin Giu Pro Ile Giu Thr Leu Ile Pro Ilie Ile Glu Ser Giy Ala Gin 185 190 195 ttc ctc gtc ttg ggt aaa gat gaa ttc agt aca gct caa gtt gcc acg 739 Phe Leu Vai Leu Giy Lys Asp Giu Phe Ser Thr Ala Gin Val Ala Thr 200 205 210 ttg ttg aat gaa ctc gga ctg ggg gag act cca ctg act gtg ctc agc 787 Leu Leu Asn Giu Leu Giy Leu Gly Giu Thr Pro Leu Thr Val Leu Ser 215 220 225 gat ttg ggc agt act gat gag gag atc acc caa ggc aca gct tca cat 835 Asp Leu Gly Ser Thr Asp Giu Giu Ile Thr Gin Gly Thr Ala Ser His 230 235 240 245 cca cca gct gca gtg tct gtt ctc aac gtg att gct gtg gga gct cgc 883 Pro Pro Aia Ala Val Ser Val Leu Asn Vai Ile Aia Val Gly Aia Arg 250 255 260 acc gca atg ccg aaa ccc cac ttt gaa ggc gac gta tca aac gaa gac 931 Thr Ala Met Pro Lys Pro His Phe Giu Gly Asp Val Ser Asn Giu Asp 265 270 275 ctt cgg gca ctg acc gtg gca gct cta gaa ccc acc cag gga caa atg 979 Leu Arg Ala Leu Thr Val Ala Aia Leu Giu Pro Thr Gin Giy Gin Met 280 285 290 ctg tgg acc ttc ggg gat att gga gca gca ctt gcc tgc gat tgg cta 1027 Leu Trp Thr Phe Gly Asp ile Giy Ala Ala Leu Ala Cys Asp Trp Leu 1127 WO 01/00843 WO 0100843PCTfIBOO/00923 295 cgc gca 1075 Arg Ala 310 gag caa 1123 Giu Gin gtg aaa 1171 Val Lys caa gga 1219 Gin Gly cta ggc 1267 Leu Gly 375 gga gga 1315 Gly Gly 390 ctt cac 1345 gcc ggc aac aag Ala Gly Asn Lys 315 agc caa agg aat Ser Gin Arg Asn 330 gcg cac gcc att agt Ala His Ala Ile Ser 320 gct cgc aaa cta ggt Ala Arg Lys Leu Gly 335 ttt gcc tcc atg gtt Phe Ala Ser Met Val 325 gta tcc acc ctc agt Val Ser Thr Leu Ser 340 gat atc cgc tat gta Asp Ile Arg Tyr Val gag acc Glu Thr 345 cca gaa Pro Giu ctg tca ccc aaa acg Leu Ser Pro Lys Thr 350 tca gca agc cca cat Ser Ala Ser Pro His 365 cta gtt cct gaa acc Leu Val Pro Giu Thr c tc Leu aaa Lys 355 gcc atc ttc atg aac aaa ggc Ala Ilie Phe Met Asn Lys Giy 370 gca tgg atg atg ctg cgg cct Ala Trp, Met Met Leu Arg Pro 360 atc gat Ile Asp 380 aag ctc att gcg caa gcc L~ys Leu Ile Ala Gin Ala tcc aca gaa Set Thr Glu 400 aac atc gca aag Asn Ile Ala Lys 405 aca ctc caa gaa caa cac ggc gga Giu Gin His Gly Gly Leu His Thr Leu <210> 816 <211> 415 <212> PRT <213> Corynebacteriun giutamicum <400> 816 Met Tyr Ilie Val Gly Ile Cys Leu 1 5 Met Ser Ala Pro Asp Ser Ala Pro Gln ,iy Val Val Met Ser Gin Pro Giu Arg Gly Thr His Phe Ala Val Val Ala Pro Arg Ala Pro Ala Asp Giy Phe Giu Gly Leu 70 Ala Ser Val Val Ilie Gly Gly Giu 55 G ly Aso Ser Gin Asp Pro Ile Thr Leu Ile Lys Ala Gin Gin 75 Ala Giy Ala His Giu Arg Gin Ala Thr Ile Gly Thr Leu Gin Arg Val Pro Asp Leu Ser Trp, Arg Leu Asn Leu 1128 WO 01/00843 WO 0100843PCTIiBOO/00923 Ala Ile Lys Ala Giu Arg Arg Pro 100 Trp Pro Gly Asn Thr Lys His Pro 105 110 Asp Leu His 145 Ala Val Glu Ala Leu 225 Gly Ala Val Thr Ala 305 Phe Val1 Asp Phe Met 385 Asp Leu Ala 130 Val Ser Val1 Ser Gin 210 Thr Thr Val1 Ser Gin 290 Cys Ala Ser Ilie Met 370 Met Asn Asp 115 Ser Leu Leu Tyr Gly 195 Val Val1 Ala Gly Asn 275 Gly Asp Ser Thr Arg 355 Asn Leu Ile Ala Gly Gly Ala Leu 180 Al a Ala Leu Ser Ala 260 Giu Gin Trp Met Leu 340 Tyr Lys Arg Al a Leu Asp Met Cys 165 Gly Gin Thr Se r His 245 Arg Asp Met Leu Val1 325 Ser Val1 Gly Pro Lys 405 Phe Pro Asp 150 Ala Gin Phe Leu Asp 230 Pro Thr Leu Leu Arg 310 Glu Val1 Gin Leu Gly 390 Leu Lys Leu 135 Arg Arg Glu Leu Leu 215 Leu Pro Ala Arg Trp 295 Ala Gin Lys Gly Gly 375 G ly His Giu 120 Phe Leu Leu Pro Val 200 Asn Gly Ala Met Ala 280 Thr Ala Ser Giu Pro 360 Ile Lys Thr Phe Tyr Thr Gly Ile 185 Leu Giu Ser Ala Pro 265 Leu Phe Gly Gin Thr 345 Glu Asp Leu Leu Arg Gly 140 Pro Val Leu Asp Leu 220 Glu Val His Ala Ile 300 Ala Ala Pro Ser Pro 380 Gin Gin His 125 Thr Gly Asn Ile Giu 205 Gly Giu Leu Phe Ala 285 Gly His Arg Lys Pro 365 Glu Ala His Val Ala Pro Arg Pro 190 Phe Glu Ile Asn Giu 270 Leu Ala Ala Lys Thr 350 His Thr Ser G ly Ala Met Ser Thr 175 Ile Ser Thr Thr Val1 255 Gly Giu Ala Ile Leu 335 Leu Ala Ala Thr Gly 415 Val Val Ser 160 Arg Ile Thr Pro Gin 240 Ile Asp Pro Leu Ser 320 Gly Lys Ile Trp Giu 400 1129 WO 01/00843 WO 0100843PCTIBOOIOO923 <210> 817 <211> 873 <212> DNA <213> Corynebacteriumgiutanicun <220> <221> <222> <223>

CDS

(101)..(850) RXA0 1692 <400> 817 gggctgatgc gaagtcagct tcgagtttga aaggtgcagt attcatggtt caagcctagt gttcgggttc tggcaattgt tgggactagg attaatgccc atg acg att Met Thr Ile tcc caa Ser Gin ggt cct Gly Pro gaa aac cag cca ata atc cag cca gtc Giu Asn Gin Pro Ile Ilie Gin Pro Vai tta att ggt gga Leu Ile Gly Gly ggt gca tgg Gly Ala Trp gct gat gtc Ala Asp Vai tta att acg gtg Leu Ile Thr Val ggg atg aat cgc Giy Met Asn Arg ctt cag gag Leu Gin Giu gag ttg gaa Giu Leu Giu att ttg gct gat Ile Leu Ala Asp ttg ggg ccc act Leu Gly Pro Thr aaa ttg Lys Leu tgc gac atc agc Cys Asp Ilie Ser aag act gtt gtt Lys Thr Vai' Val gtg tcc aag ctt Val Ser Lys Leu ccg Pro tat ggg cgg cag Tyr Gly Arg Gin act cag gag cgt Thr Gin Giu Arg aat gag atg ctt Asn Giu Met Leu 115 163 211 259 307 355 403 451 499 547 595 643 gaa tac gca cag Giu Tyr Aia Gin gga cta aag gtg Giy Leu Lys Val cgc ctt aaa ggt Arg Leu Lys Giy ggt gac Giy Asp 100 cct tat gtc Pro Tyr Val cat gga att His Gly Ilie 120 ttc Phe 105 ggt cgg ggt ttt gaa gag ttg gag ttt Giy Arg Gly Phe Giu Giu Leu Giu Phe 110 ttg ggc gag Leu Giy Giu 115 gcg gtg tcc Ala Val Ser gaa tgc gag gtc Giu Cys Giu Val att Ile 125 ccg ggt gtg acc Pro Giy Val Thr gtt cca Vai Pro 135 gcg gcg gca ggt Ala Ala Ala Giy att Ile 140 cct att act aat Pro Ilie Thr Asn cgg Arg 145 gga gtg gtg cat Giy Val Val His ttt acc gtg gtg Phe Thr Val Val tct Ser 155 gga cat ttg cct Giy His Leu Pro cca Pro 160 ggc cat ccg aag Gly His Pro Lys tca Ser 165 ctg gtt gat tgg Leu Vai Asp Trp gct Aia 170 gcg ttg gcc aaa Ala Leu Ala Lys tcg Ser 175 ggt ggc acc ttg Giy Gly Thr Leu tcc atc Ser Ile 180 1130 WO 01/00843 WO 0100843PCT/IBOO/00923 atc Ile Gly act Thr gtc Val 230 caa Gin gg t Gly ct t Leu 200 gca Ala gtg Val1 gc t Ala aaa aat gcg ggt gcg att gcc cag Lys Asn Ala Gly Ala Ile Ala Gin 190 gca gat act cca gca gct gtt att Ala Asp Thr Pro Ala Ala Val Ile 205 cgc tca gtt cgg tgc acc ttg ggc Arg Ser Val Arg Cys Thr Leu Gly 220 225 gaa gag att aag cct cca gct gtg Giu Giu Ile Lys Pro Pro Ala Val 235 240 ctc taagcagatc gcctaagaat ggg Leu 250 gcg ctc Ala Leu 195 cag gaa Gin Giu 210 aca ttg Thr Leu atg gac Met Asp ggc act Gly Thr ggt gca Gly Ala tat gtc att Tyr Val Ile <210> 818 <211> 250 <212> PRT <213> Corynebacterium giutamicun <400> 818 Met Thr Ile Ser Gin Giu Asn Gin Pro 1 5 Ile Giy Gly Gly Pro Gly Ala Trp Asp 25 Asn Arg Leu Gin Giu Ala Asp Val Ile 40 Thr Asp Giu Leu Giu Lys Leu Cys Asp 55 Asp Vai Ser Lys Leu Pro Tyr Gly Arg Asn Giu Met Leu Val Giu Tyr Ala Gin Leu Lys Gly Gly Asp Pro Tyr Val Phe 100 i05 Giu Phe Leu Giy Giu His Gly Ile Giu 115 120 Thr Ser Ala Vai Ser Val Pro Ala Ala 130 135 Arg Gly Val Val His Ser Phe Thr Val 145 150 Gly His Pro Lys Ser Leu Val Asp Trp 165 Ile 10 Leu Leu Ile Gin Gin 90 Gly Cys Ala Val1 Ala 170 Gin Thr Asp Ser Thr Leu Gly Val Ile 140 Giy Leu Pro Val His Lys Gin Lys Phe Ile 125 Pro His Ala Val1 Arg Leu Thr Giu Val Giu 110 Pro Ile Leu Lys Ser Gly Gly Vai Arg Val Giu Giy Thr Pro Ser 175 1131 WO 01/00843 Gly Thr Leu Gin Ala Leu 195 Ile Gin Giu 210 Gly Thr Leu 225 Val Tyr Val Ile Asp Thr Ala Gly 245 Ile Met Gly Val 185 Gly Gly Leu Asp 200 Thr Asp Ala Gin 215 Vai Met Val Giu 230 Gin Val Ala Gly PCT/IBOO/00923 Lys Asn Ala Giy Ala Ile Aia 190 Aia Asp Thr Pro Aia Ala Val 205 Arg Ser Val Arg Cys Thr Leu 220 Giu Giu Ile Lys Pro Pro Ala 235 240 Leu 250 <210> 819 <211> 1917 <212> DNA <213> Corynebacteriun giutamicun <220> <221> CDS <222> (101)..(1894) <223> RXN00371 <400> 819 gtcgtggaca atcccggatc gaaaatttga ttcggctttt ttcatggctg ttgatggagt acgttggtcg ttttcgagac aagtactaga aaagatattg atg act atc gcc cat met Thr Ile Ala His aag Lys gaa Giu tca Ser ggc Gly aca Thr ga t Asp ccc Pro acc Thr tct Ser aaa Lys gtt Val1 gaa Glu gag Giu atc Ile ttt Phe gtc Val1 cgt Arg caa Gin gct Ala gt t Val1 gga Gly ttt Phe cgt Arg cta Leu gaa Giu gaa Giu cag Gin gtt Val gaa Giu 75 agc Ser act Thr tcg Ser cat His ggg Gly gt t Val ggc Gly ac c Thr ctc Leu gag Giu gcc Ala c tg Leu gt t Val ggg Gly acg Thr gga Gly ggt Gly ggc Gly cga Arg gct atc Ile i5 cac His caa Gin ccc Pro aac Asn gc t Al a 95 gcg Ala gag Giu gga Gly acg Thr ggt Gly gcg Ala 80 ttt Phe gaa Giu cag Gin cgt Arg tta Leu gat Asp gcg Ala act Thr tac Tyr 115 gtt Val cc t Pro ctt Leu ctt Leu att Ile gaa Giu 100 gag Glu tcc Ser gtt Val1 cca Pro ct t Leu act Thr att Ile cgc Arg 115 163 211 259 307 355 403 451 cct gtg ccg gaa gat aag ctt cag Pro Val Pro Giu Asp Lys Leu Gin Ala 1132 WO 01/00843 atc tgc at Ile Cys I] cca gca cc Pro Ala Pi 135 cca gct cz Pro Ala GI 150 gat gtt at Asp Val I] ctg gct ge Leu Ala G] gtt cca gc Val Pro GI gcg ttg gc Ala Leu GI 215 gac tgg ge Asp Trp A~c 230 cgc gtg gi Arg Val A., atg tct ti Met Ser iA ttg cag cc Leu Gin A3 gct gaa ct 1027 Ala Glu IA 295 gat cgc tc 1075 Asp Arg S( 310 cgt gtg ct 1123 Arg Val L( cgt ctg ac 1171 gag Glu gca Ala 140 ctt Leu acc Thr gtt Val cct Pro acc Thr 220 agc Ser cgt Arg gtt Val1 acc Thr ttg Leu 300 tgg Trp gc t Ala gct aac Asn 125 gag Gi u gtg Val1 ggc Gly tcC Ser gca Al a 205 gaa Glu gca Al a att Ile tct Ser act Thr 285 gtt Val tgg Trp cgg Arg atc cgc Arg gaa Glu gc t Ala 160 t tg Leu ggc Gly cc t Pro gtc Val1 cct Pro 240 gaa Glu gc t Ala ctg Leu ttg Leu cgc Arg 320 gcg Al a gaa cgt Arg gtt Val 145 ctt Leu agc Ser ctg Leu gca Ala aac Asn 225 ttg Leu cta Leu aac Asn ttg Leu 9gc Gly 305 gc t Ala gca Ala gtc cct Pro gag Glu tat Tyr gat Asp ttc Phe 195 gcg Al a c aa Gl n ctg Leu gcc Ala acc Thr 275 aag Lys ggt Gly tac Tyr atg Met acc PCT/iBOO/00923 cct cgt 499 Pro Arg gcg act 547 Ala Thr ggt gga 595 Gly Gly 165 gcc aca 643 Ala Thr 180 cag gtg 691 Gln Val gga att 739 Gly Ile aac ttg 787 Asn Leu cag gcc 835 Gln Ala 245 cgc aat 883 Arg Asn 260 acc cgt 931 Thr Arg ctt gat 979 Leu Asp gtg gat Val Asp ggt tgg Gly Trp, 325 tcc gca Ser Ala 340 att tct 1133 WO 01/00843 Arg Leu Ser PCT/EB00100923 His Gly Ala Ile Gin Glu Val Pro Thr Ile Ser 355 gtc gaa 1219 Vai Giu atc gtc 1267 Ilie Val 375 gtg aag 1315 Val1 Lys 390 tcc ttc 1363 Ser Phe gag atc 1411 Giu Ile agg caa 1459 Arg Gin gaa ctc 1507 Giu Leu 455 acc gac 1555 Thr Asp 470 gac gtg 1603 Asp Vai atc cga 1651 Ile Arg tct tcg 1699 Ser Ser cca cgc 1747 Pro Arg ccg cgc aac cca Pro Arg Asn Pro gga cgc tac cag Gly Arg Tyr Gin gcg Aia 365 atg gaa cgc gcc atc aag ggc Met Giu Arg Ala Ile Lys Giy 370 gca gtc tgg gag Aia Val Trp Glu 395 gcg ggc gtc cgc Ala Gly Val Arg tgg gtt gtc ctc Trp Val Vai Leu atc acc gaa ttc Ile Thr Giu Phe 400 gcc gca gtc ggt Ala Ala Val Gly acc Thr 385 ggc ctc gat tca cgt Gly Leu Asp Ser Arg 405 gaa aaa acc gcc gct Giu Lys Thr Ala Ala 420 agc gtc aac gca Ser Val Asn Ala atc Ile cgc gcg Arg Ala 425 aat gcg Asn Ala 410 415 ctc ggc atc acg ccg Leu Gly Ilie Thr Pro 430 caa ggg ctt gtc gac Gin Gly Leu Val Asp gag ctt ctg cct gca cgt acc Giu Leu Leu Pro Ala Arg Thr 435 gtg ttc ccc gaa tat ttc gaa Val Phe Pro Giu Tyr Phe Giu 450 ttg ccg cgc gca gat atc gca Leu Pro Arg Ala Asp Ile Ala cca gtc ggc cgt Pro Val Gly Arg 460 ctc Leu 465 gtg ctt gtc gac Val Leu Val Asp 475 gtg gct tac cgc Val Ala Tyr Arg 490 gat atg atc aag Asp Met Ile Lys ggc ctg acc cac ctt Gly Leu Thr His Leu 480 acc gtc cgc gca gca Thr Val Arg Ala Ala 495 acc ggc gga ttt gat Thr Gly Gly Phe Asp ggt Gly tgg gaa gtc gaa Trp Giu Val Giu 485 cca cca agc gct gat Pro Pro Ser Ala Asp 500 gca gtt gcc ttc acc Ala Val Ala Phe Thr tcg Ser 505 510 515 acc gtg aag aac ctc Thr Val Lys Asn Leu 525 atc gtc gcg tgc atc Ile Val Ala Cys Ile gtt ggt atc gcg ggt aaa cca cac Val Gly Ile Ala Gly Lys Pro His 530 gga ccc atg act gca gcg acc gct Gly Pro Met Thr Ala Ala Thr Ala 1134 WO 01/00843 535 540 gaa gaa ctg gga ctg cgc gtt gq 1795 Giu Giu Leu Gly Leu Arg Val A, 550 555 cca gaa ctg atc gac gct ctt g 1843 Pro Giu Leu Ile Asp Ala Leu A 570 aag ggc gag ctg ccg ccg ccg a, 1891 Lys Gly Glu Leu Pro Pro Pro A 585 tct taaaaggttt ttcactaggg tgt 1917 Ser PCT/IBOO/00923 gtc atg cca Val Met Pro 560 gaa cac gtg Giu His Val 575 aag aaa cgc Lys Lys Arg 590 545 gag atc Glu Ile gcg gat Ala ASP agg cgt Arg Arg gcc Ala ctg Leu cga Arg 595 <210> 820 <211> 598 <212> PRT <213> Corynebacterium glutainicum <400> 820 Met Thr Ile Ala His Lys Pro Giu Met 1 Thr Asn Glu Asn Val1 Val1 Asp Arg Val1 145 Leu Asn Leu Glu Pro Arg Ala Glu Lys 130 Ser Ser Gin Arg Leu Asp Ala Thr Tyr 115 Pro Giu Tyr Val1 Pro Leu Leu Ile Glu 100 Giu Pro Al a Gly 5 Ser Val Pro Leu Thr Ile Arg Arg Thr Gly 165 Ala Val1 Gly Phe Arg Leu Asp Ala Thr Val1 Leu 170 Thr Ser His Gly Val Gly Leu Glu Ala 140 Leu Thr Thr Leu G lu Ala Leu Val1 Gin Asn 125 Giu Val1 Gly 1135 WO 01/00843 WO 0100843PCT.LBOO/00923 Ser Ser Asp Ala Thr Leu Ala Giu Ile Ser Ala Val Ser Giu Al1a Giy 180 185 190 Leu Glu Phe Gin Val Val Pro Gly Met Ser Leu Pro Ala Thr Val Pro 195 200 205 Ala Phe Ala Gly Ile Ala Leu Gly Ser Thr Tyr Thr Glu Thr Asp Vai 210 215 220 Asn Gly Gln Asn Leu Asp Trp Asp Gin Leu Ala Ser Ala Pro Gin Pro 225 230 235 240 Leu Val Leu Gin Ala Arg Val Asp Asp Leu Ser Arg Ile Ala Gin Giu 245 250 255 Leu Lys Ala Arg Asn Met Ser Leu Giu Thr Pro Val Ser Val Thr Ala 260 265 270 Asn Gly Thr Thr Arg Leu Gin Arg Thr Tyr Asp Thr Thr Leu Gly Leu 275 280 285 Leu His Lys Leu Asp Ala Giu Leu Ser Gly Pro Leu Val Val Thr Leu 290 295 300 Gly Lys Giy Val Asp Asp Arg Ser Lys Tyr Ser Trp Trp Giu Asn Arg 305 310 315 320 Ala Leu Tyr Gly Trp Arg Val Leu Val Pro Arg Ala Arg Giu Gin Ala 325 330 335 Ala Ser Met Ser Ala Arg Leu Ser Ser His Gly Ala Ile Pro Gin Giu 340 345 350 Val Pro Thr Ile Ser Val Glu Pro Pro Arg Asn Pro Ala Gin met Giu 355 360 365 Arg Ala Ilie Lys Gly Ile Val Giu Gly Arg Tyr Gin Trp, Val Val Leu 370 375 380 Thr Ser Val Asn Ala Val Lys Ala Val Trp Giu Lys Ile Thr Giu Phe 385 390 395 400 Gly Leu Asp Ser Arg Ser Phe Ala Gly Val Arg Ile Ala Ala Val Gly 405 410 415 Giu Lys Thr Ala Ala Giu Ile Arg Ala Leu Gly Ile Thr Pro Giu Leu 420 425 430 Leu Pro Ala Arg Thr Arg Gin Asn Ala Gin Gly Leu Val Asp Val Phe 435 440 445 Pro Giu Tyr Phe Giu Giu Leu Asp Pro Val Gly Arg Val Leu Leu Pro 450 455 460 Arg Ala Asp Ilie Ala Thr Asp Val Leu Val Asp Gly Leu Thr His Leu 465 470 475 480 Gly Trp Giu Val Giu Asp Val Val Ala Tyr Arg Thr Val Arg Ala Ala 485 490 495 1136 WO 01/00843 Pro Pro Se Ala Val Al 51 Ala Gly L) 530 Thr Ala A] 545 Glu Ile A] Ala Asp LE Arg Arg Ax Arg Ser Arg 535 Glu Glu Gly Asp Ser 520 Thr Leu Leu Glu Met 505 Thr Ile Gly Ile Leu 585 Gly Leu 525 Ile Asp Ala Arg Gly 510 Val Gly Val Giu Lys 590 PCTAB0O/00923 Phe Asp Gly Ilie Pro met Met Pro 560 His Val 575 Lys Arg <210> 821 <2-11> 1024 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS <222> (101)..(1024) <223> FRXA00371 <400> 821 gtcgtggaca atcccggatc gaaaatttga ttcggctttt acgttggtcg ttttcgagac aagtactaga aaagatattg aag Lys gaa Giu tca Ser ggc Gly aca Thr ga t Asp act Thr tcg Ser cat H-is ggg Gly gtt Val1 Gly acc Thr c tc Leu gag Glu gcc Ala ctg Leu gtt Val ggg Gly acg Thr gga Gly ggt Gly ggc Gly cga Arg 1137 ttcatggctg ttgatggagt atg act atc gcc cat Met Thr Ile Ala His 1 acc aac cag gtt tcc Thr Asn Gin Val Ser aac tta cgt cct gtt Asn Leu Arg Pro Val gaa gag tta ctt cca Giu Giu Leu Leu Pro aac cct gat ctt ctt Asn Pro Asp Leu Leu gtt cgt gcg att act Val Arg Ala Ile Thr gtc gcc act gaa. att Val Ala Thr Giu Ile WO 01/00843 WO 0100843PCTIIBOOIOO923 cct gtg ccg Pro Val Pro gaa Giu 105 gat aag ctt cag Asp Lys Leu Gin gc t Ala 110 gcg gaa gat gag Ala Giu Asp Glu tac gag cgc Tyr Giu Arg 115 cct cct cgt Pro Pro Arg atc tgc att gaa gcg aag gag Ile Cys Ile Giu Ala Lys Giu 120 aac Asn 125 ggt gca cgc cgt Gly Ala Arg Arg cca gca Pro Ala 135 cca cca acc gct Pro Pro Thr Ala gca Ala 140 gag atc acg gaa gtt tct gag gcg act Glu Ile Thr Giu Val Ser Giu Ala Thr 145 gct cag att gtt Ala Gin Ile Val ctt gtg cag gat Leu Val Gin Asp ctt tct tat ggt Leu Ser Tyr Gly gga Gly 165 gat gtt att cgt ASP Vai Ile Arg gtc acc ggc aac Val Thr Gly Asn ttg agc agc gat Leu Ser Ser Asp gcc aca Aia Thr 180 ctg gct gag Leu Ala Giu gtt cca ggt Val Pro Gly 200 tct gca gtt tcc Ser Ala Val Ser gct ggc ctg gag Ala Gly Leu Giu ttc cag gtg Phe Gin Val 195 gcg gga att Ala Gly Ilie atg tct ttg cct gca acg gtt cct gca Met Ser Leu Pro Ala Thr Val Pro Aia 205 gcg ttg Ala Leu 215 ggt tct acc tac' Gly Ser Thr Tyr acc Thr 220 gaa act gat gtc Giu Thr Asp Val ggt caa aac ttg Gly Gin Asn Leu gac Asp 230 tgg gat cag ttg Trp Asp Gin Leu gct Ala 235 agc gca cct cag Ser Ala Pro Gin ttg gtg ctg cag Leu Val Leu Gin cgc gtg gat gac Arg Val Asp Asp tcc cgt att gca Ser Arg Ile Ala gaa cta aag gcc Giu Leu Lys Ala cgc aat Arg Asn 260 atg tct ttg Met Ser Leu ttg cag cgc Leu Gin Arg 280 act cct gtt tct Thr Pro Val Ser gtC Vai 270 acc gct aac ggc Thr Ala Asn Gly acc acc cgt Thr Thr Arg 275 aag ctt gat Lys Leu Asp acc tat gac acc Thr Tyr Asp Thr act Thr 285 tta ggt ctg ttg Leu Gly Leu Leu gct gaa cta agc gga cct ttg gtt gtt acc ttg ggc aag ggt gtg' 1024 Ala Giu 295 Leu Ser Gly Pro Leu 300 Val Val Thr Leu Lys Gly Val <210> 822 <211> 308 <212> PRT <213> Corynebacteriun giutamicun 1138 WO 01/00843 <400> 822 met Thr Ii 1 Thr Asn Gi Asn Leu Ar 3 Glu Glu Le Asn Pro As Val Arg Al Val Ala Tb Asp Glu Ty 11 Arg Lys Pr 130 Val Ser Gl 145 Leu Ser Ty Ser Ser As Leu Glu Ph 19 Ala Phe Al 210 Asn Gly Gi 225 Leu Val Le Leu Lys Al Asn Gly Tb 27 Leu His Ly 290 Gly Lys Gl 305 PCT/IBOO/00923 e n g 5 u p a 'r r '0 u *r 'p '5 a ir '5 '5 Ala Val Pro Leu Leu I le Glu 100 Giu Pro Ala Gly Ala 180 Gin Gly Asn Gin Arg 260 Thr Leu Val1 His 5 Ser Val1 Pro Leu Thr Ile Arg Arg Thr Gly 165 Thr Val1 Ile Leu Al a 245 Asn Arg Asp Lys Giu Ser Gly Thr 70 Asp Pro Ile Pro Pro 150 Asp Leu Val Ala Asp 230 Arg Met Leu Ala Pro Thr Ser Lys 55 Val1 Glu Val Cys Ala 135 Ala Val1 Ala Pro Leu 215 Trp Val1 Ser Gln Giu 295 Giu Ile Phe Val Arg Gin Pro Ile 120 Pro Gin Ile Giu Gly 200 Gly Asp Asp Leu Arg 280 Leu Giu Glu Gin Val1 Glu 75 Ser Lys Lys Ala Giu 155 Val Ala Leu Tyr Ala 235 Ser Pro Asp Pro Thr Ser His Gly Val Gly Leu Glu Ala 140 Leu Thr Val Pro Thr 220 Ser Arg Val1 Thr Leu 300 Gly Thr Gly Gly Gly Arg Ala 110 Gly Ile Gin Asn Glu 190 Thr Thr Pro Ala Val1 270 Leu Val1 Ile His Gin Pro Asn Ala Ala Ala Thr Asp Pro 175 Ala Val Asp Gln Gin 255 Thr Gly Thr Glu Gly Thr Gly Ala Phe Giu Arg Giu Ala 160 Leu Gly Pro Val Pro 240 Giu Ala Leu Leu 1139 WO 01/00843 WO 0100843PCT/EB00100923 <210> 823 <211> 755 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> <223> FRXA00374 <400> 823 acc att tct gtc Thr Ile Ser Val 1 atc aag ggc atc Ile Lys Gly Ile gtc aac gca gtg Val Asn Ala Val gat tca cgt tcc Asp Ser Arg Ser acc gcc gct gag Thr Ala Ala Glu gca cgt acc agg Ala Arg Thr Arg tat ttc gaa gaa Tyr Phe Giu Giu 100 gat atc gca acc Asp Ilie Ala Thr 115 gaa gtc gaa gac Giu Val Glu Asp 130 agc gct gat atc Ser Ala Asp Ilie 145 gcc ttc acc tct Ala Phe Thr Ser aaa cca cac cca Lys Pro His Pro 180 cca ccg cgc aac cca gcg caa atg gaa cgc gcc Pro Pro Arg Asn Pro Ala Gin Met Giu Arg Ala gaa Giu gca Ala gcg Ala cgc Arg 70 aat Asn ga t Asp gtg Val1 g tg Val1 gat Asp 150 tcg Ser acc Thr gga Gly gtc Val ggc Gly 55 gcg Ala gcg Ala cca Pro ctt Leu gc t Ala 135 a tg Met acc Thr a tc Ile cgc Arg tgg Trp 40 gtc Val ctc Leu caa Gin gtc Val gtc Val1 120 tac Tyr atc Ile gtg Va 1 gtc Val1 tac Tyr 25 aag Lys cgc Arg ggc Gly ggg Gly ggc Gly 105 gac Asp cgc Arg aag Lys aag Lys gcg Ala 185 cag Gin aaa Lys atc Ile atc Ile ctt Leu cgt Arg ggc Gly acc Thr acc Thr aac Asn 170 tgc Cys tgg Trp atc Ile gcc Ala acg Thr 75 gtc Val gtc Val c tg Leu gtc Val ggc Gly 155 C tc Leu atc Ile gtt Val1 acc Thr gca Ala ccg Pro gac Asp c tc Leu acc Thr cgc Arg 140 gga Gly gtt Val1 gga Gly gtc Val gaa Glu gtc Val1 gag Giu gtg Val ttg Leu cac His 125 gca Ala ttt Phe ggt Gly ccc Pro CtC Leu ttc Phe ggt Gly ctt Leu ttc Phe ccg Pro 110 Ctt Leu gc a Ala gat Asp atc Ile a tg Met 190 aca Thr ggC Gly gaa G iu ctg Leu

CCC

Pro cgc Arg ggt G ly cca Pro gca Ala gcg Ala 175 act Thr agc Ser ctc Leu aaa Lys CCt Pro gaa Glu gca Ala tgg Trp cca Pro gtt Val1 160 ggt G ly gca Ala 96 144 192 240 288 336 384 432 480 528 576 624 gcg acc gct gaa gaa ctg gga ctg cgc gtt gat gtC atg cca gag atc 1140 WO 01/00843 Ala Thr Ala 195 gcc gaa gta Ala Glu Val 210 ctg cgc gct Leu Arg Ala 225 cga aaa gcg Arg Lys Ala Glu cca Pro aag Lys tct Ser PCTIBOO/00923 Giu Leu Gly Leu Arg Val Asp Vai Met Pro Glu Ile 200 205 gaa ctg atc gac gct ctt gcg gaa cac gtg gcg gat 672 Glu Leu Ile Asp Ala Leu Ala Glu His Val Ala Asp 215 220 ggc gag ctg ccg ccg ccg agg aag aaa cgc agg cgt 720 Gly Glu Leu Pro Pro Pro Arg Lys Lys Arg Arg Arg 230 235 240 taaaaggttt ttcactaggg tgt 755 <210> 824 <211> 244 <212> PRT <213> Corynebacteriun glutamicumr <400> 824 Thr Ile Ser Val Glu Pro Pro Arg Asn 1 Ile Vai Asp Thr Al a Tyr Asp Glu Ser 145 Al a Lys Ala Lys Asn Ser Ala Arg Phe Ile Val 130 Ala Phe Pro Thr Ile Val Ser Glu Arg Giu 100 Thr Asp Ile Ser Pro 180 Glu 5 Val Lys Phe Ile Gin Leu Asp Val1 Arg Ser 165 Arg Glu Giu Al a Ala Arg 70 Asn Asp Val Val1 Asp 150 Ser Thr Leu Gly Val Gly 55 Ala Ala Pro Leu Ala 135 Met Thr Ile Gly Arg Trp Val Leu Gin Val Val 120 Tyr Ile Val Val Leu 200 Tyr Lys Arg Gly Gly Gly 105 Asp Arg Lys Lys Ala 185 Arg Pro 10 Gin

LYS

Ile Ile Leu 90 Arg G ly Thr Thr Asn 170 Cys Val Ala Trp Ile Ala Thr 75 Val Val Leu Val Gly 155 Leu Ile Asp Gin Vai Thr Ala Pro Asp Leu Thr Arg 140 Gly Val1 Gly Val1 Met Val1 Giu Val Glu Val Leu His 125 Ala Phe Gly Pro Met 205 Arg Thr Gly Glu Leu Pro Arg Gly Pro Ala Ala 175 Thr Glu Ala Ser Leu Lys Pro Glu Ala Trp Pro Val 160 Gly Ala Ile 1141 WO 01/00843 WO 0100843PCTIBOO/00923 Ala Giu Val Pro Giu Leu Ile Asp Ala Leu Ala Glu 210 215 220 Leu Arg Ala Lys Gly Glu Leu Pro Pro Pro Arg Lys 225 '230 235 His Val Ala Asp Lys Arg Arg Arg Lys Ala Ser <210> 825 <211> 1467 <212> DNA <213> Corynebacteriun <220> <221> CDS <222> (101)..(1444) <223> RXN00383 <400> 825 ccatatcttt aaccttggtc giutanicun atggtgtgct tcctaatacg gtggcggaag atattactga agccgtctcc atcattcatt cttaaactaa gaggagtttc atg cgt ttt gcc Met Arg Phe Ala atc ggt gca ggc Ile Gly Ala Gly gcg ggt ctg act gct gca tat gag atc Ala Gly Leu Thr Ala Ala Tyr Giu Ile cat aaa His Lys gct gat ccc Ala Asp Pro ggc ggc aag Gly Gly Lys gcc caa atc gat Ala Gin Ile Asp ttg gaa gca ggc Leu Glu Ala Gly gaa cgc att Glu Arg Ile acc gat att Thr Asp Ile ctt ttt acg gtg Leu Phe Thr Val ttt gct tcc gga.

Phe Ala Ser Gly gga gcg Gly Ala gag gcg ttt tta Glu Ala Phe Leu gcg cgt tcc gat Ala Arg Ser Asp gtg gag ttt ttt Val Glu Phe Phe act Thr gag ctt ggg ttg Glu Leu Gly Leu gc t Ala gat tct ttg gtc Asp Ser Leu Val ccg tct gct gcg Pro Ser Ala Ala tct cag tat ttc Ser Gin Tyr Phe ggc ggt gcg ctg cat gcg ttc ccc gca Gly Gly Ala Leu His Ala Phe Pro Ala ggt gga Gly Gly 100 gtg atg ggt Val Met Gly ttt gat tgg Phe Asp Trp 120 cca agc aat cca Pro Ser Asn Pro gca ggc gcg caa gac acc gct Ala Gly Ala Gin Asp Thr Ala act cct ggc caa Thr Pro Gly Gin gac att tct gtt ggc gcc tta gtg cgc Asp Ilie Ser Val Gly Ala Leu Val Arg 125 *130 cgt cag tat ggc gat gag atc gtc gat act gtg gtg tct tcg ctg ctt Arg Gin Tyr Gly Asp Giu Ilie Val Asp Thr Val Val Ser-Ser Leu Leu WO 01/00843 135 ggt ggc gtt Gly Gly Val 150 gtg ccg gca Val Pro Ala ccg gtg act Pro Val Thr gcc gca aaa Ala Ala Lys 200 ggc gga tac Gly Gly Tyr 215 gat att cac Asp Ile His 230 ggt ttt gcc Gly Phe Ala ttg gcg gtt Leu Ala Val ccg gcc gca Pro Ala Ala 280 gtc gtc ggc 1027 Val Val Gly 295 ggc gtc ctg 1075 Gly Val Leu 310 acg ttc tcc 1123 Thr Phe Ser ctc gtg cgc 1171 Leu Val Arg atg gac gag 1219 Met Asp Glu PCT/EBOO/00923 tat Tyr ctt Leu ctg Leu 185 aca Thr gcg Ala ttg Leu atc Ile ccc Pro 265 gcg Ala atg Met gtc Val1 tca Ser gcg Ala 345 gat Asp tcc Ser gc t Ala 170 tca Ser act Thr gag Glu gat Asp aag Lys 250 gc t Ala cca Pro cgt Arg gct Ala aag Lys 330 tcg Ser t tg Leu tcc Ser 155 gca Ala gct Ala tca Ser ctg Leu agt Ser 235 ggc Gly cca Pro cat His ttc Phe gtt Val1 315 aag Lys ttc Phe ctt Leu 140 acc Thr gcc Ala gcg Ala gaa Giu tac Tyr 220 ttc Phe gg t G ly acc Thr ttg Leu gat Asp 300 aat Asn tgg Trp, ggc Gly gtc Val1 gat Asp gat Asp aag Lys 190 cgc Arg gcg Ala tcc Ser gaa Glu gct Ala 270 gca Ala agc Ser ccg Pro cac His cta Leu 350 gcc Ala ga t Asp cag Gin 175 gcc Ala ccc Pro ttg Leu gcC Ala ggc Gly 255 gtg Val att Ile gag Glu ggc Gly ctg Leu 335 ggc Gly gcc Ala ctg Leu 160 ctg Leu gtg Val gtt Val1 gca Ala atc Ile 240 acc Thr ctg Leu aag Lys ggc Gly atc Ile 320 gag Giu gat Asp c tc Leu 145 gg t Gly gct Ala gaa Glu t tc Phe gag Glu 225 ac c Thr tac Tyr ctc Leu t tg Leu ctg Leu 305 acg Thr gc t Ala gag Glu gac Asp gct Ala ggC Gly 180 cgg Arg ttc Phe ggt Gly ggt Gly gtg Val 260 t tg Leu tca Ser aac Asn gcc Ala ggc Gly 340 gca Ala ctc Leu 595 643 691 739 787 835 883 931 979 1143 WO 01/00843 WO 0100843PCT[IBOO/00923 360 365 ata acc ggg ttc gac ggc cgg gct gcc 1267 Ile Thr Gly Phe Asp Gly Arg Ala Ala 375 380 cag cgc tgg ttc ggt ggg ctc cca gcc 1315 Gin Arg Trp, Phe Gly Gly Leu Pro Ala 390 395 acc gtt tcg gct gcg cgt gca gag atc 1363 Thr Val Ser Ala Ala Arg Ala Glu Ile 410 gca att ggc gcg tgg gct ggg gga gtg 1411 Ala Ile Gly Ala Trp Ala Gly Gly Val 425 430 gat gcc cag gca gca gta cac agg ttg 1464 Asp Ala Gin Ala Ala Val His Arg Leu 440 445 tga 1467 <210> 826 <211> 448 <212> PRT <213> Corynebacteriun giutainicum <400> 826 Met Arg Phe Ala Ile Ile Gly Ala Gly 1 5 Tyr Giu Ile His Lys Ala Asp Pro Thr Ala Gly Glu Arg Ile Gly Gly Lys Leu Gly Pro Thr Asp Ile Gly Ala Giu Ala Ala Val Glu Phe Phe Thr Giu Leu Gly 70 Pro Ser Ala Ala Lys Ser Gin Tyr Phe Phe Pro Ala Gly Gly Val Met Gly Ile 100 105 Ala Gin Asp Thr Ala Phe Asp Trp Thr 115 120 gga Gly tat Tyr gca Ala 415 gga Gly ctg Leu Leu 10 Ala Phe Phe Leu Ala 90 Pro Pro ctg Leu gga Gly 400 gcc Ala gtt Val gga Gly Ala Gin Thr Leu Ala 75 Gly S er Gly 370 ggt gaa att ttc gtg Gly Giu Ile Phe Val 385 gtt gat cac att gct Val Asp His Ile Ala 405 gtg cct ggc gtg gaa Val Pro Gly Val Glu 420 ccc gca gtt atc gca Pro Ala Val Ile Ala 435 taagcaccca aaaacactat Ala Leu Ala Ser Val1 His Ala Ser Ala Glu Ser Asp Ser Ala Gly Val 1144 WO 01/00843 WO 0100843PCTIBOO/00923 Gly Ala Leu Val Arg Arg Gin Tyr Gly Asp Giu Ile Val Asp Thr Val 130 135 140 Val 145 Gly Ala Glu Phe Giu 225 Thr Tyr Leu Leu Leu 305 Thr Ala Glu Asp Gly 385 Val Val1 Ser Val Glu Ala Gin 210 Gin Lys Asp Arg Ala 290 Pro Ala Arg Ala Asp 370 Glu Asp Pro Ser Arg Ala Gin 195 Thr Cys Asp Lys Asp 275 Ser Asp Lys Gly Ser 355 Leu Ile His Gly Leu Ala Gly 180 Arg Phe Gly Gly Val1 260 Leu Ser Asn Ala Gly 340 Ala Leu Phe Ile Val1 420 Leu Ser 165 Glu Giu Lys Ala Glu 245 Ile Ala Ala Ser Phe 325 Ala Arg Thr Val Ala 405 Glu Gly 150 Val1 Pro Ala Gly Asp 230 Gly Leu Pro Val1 Gly 310 Thr Leu Met Ile Gin 390 Thr Ala Gly Pro Vai Aia Gly 215 Ile Phe Ala Ala Val1 295 Val Phe Val1 Asp Thr 375 Arg Val1 Ile Val1 Tyr Ala Leu Thr Leu 185 Lys Thr 200 Tyr Ala His Leu Ala Ile Val Pro 265 Ala Ala 280 Gly Met Leu Val Ser Ser Arg Ala 345 Glu Asp 360 Gly Phe Trp, Phe Ser Ala Gly Ala 425 Ser 155 Ala Ala Ser Leu Ser 235 Gly Pro His Phe Val 315 Lys Phe Leu Gly Gly 395 Arg Ala Thr Ala Ala Glu Tyr 220 Phe Gly Thr Leu Asp 300 Asn Trp Gly Val1 Arg 380 Leu Ala Gly Ala Leu Val Thr 205 Giu Val1 Gly Ala Arg 285 Ser Glu Pro Arg Asp 365 Ala Pro Glu Gly Arg 445 Asp Asp Lys 190 Arg Ala Ser Glu Ala 270 Ala Ser Pro His Leu 350 Ala Ala Ala Ile Val 430 Asp Gin 175 Ala Pro Leu Ala Gly 255 Val Ile Glu Gly Leu 335 Gly Ala Gly Tyr Ala 415 Gly Leu 160 Leu Val Val Ala Ile 240 Thr Leu Lys Gly Ile 320 Glu Asp Leu Leu Gly 400 Ala Val1 Pro Ala Val 435 Ile Ala Asp Ala Gin Ala Ala Val His 440 Leu Leu Gly 1145 WO 01/00843 WO 0100843PCT/EBOO/00923 <210> 827 <211> 382 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (101)..(382) <223> FRXA00376 <400> 827 ccatatcttt aaccttggtc atggtgtgct tcctaatacg agccgtctcc atcattcatt cttaaactaa gaggagtttc atc Ile gct Ala ggc Gly gga Gly act Thr tct Ser ggt gca ggc Gly Ala Gly gat Asp ggc Gly gcg Ala gag Giu gcg ggt Ala Gly caa atc Gin Ile acg gtg Thr Val tta gct Leu Ala 60 gct gat Ala Asp 75 ggc ggt Gly Gly ctg Leu gat Asp ccg Pro gcg Ala tct Ser gcg Ala act Thr gtg Val1 ttt Phe cgt Arg ttg Leu c tg Leu gca Al a gaa Glu tcc Ser gat Asp agc Ser 80 gtggcggaag atattactga atg cgt ttt gcc atc Met Arg Phe Ala Ile 1 tat gag atc cat aaa Tyr Glu Ilie His Lys gca ggc gaa cgc att Ala Gly Glu Arg Ile gga cct acc gat att Gly Pro Thr Asp Ile gcg gtg gag ttt ttt Ala Val Giu Phe Phe ccg tct gct gcg aag Pro Ser Ala Ala Lys cag tat ttc Gin Tyr Phe <210> 828 <211> 94 <212> PRT <213> Corynebacterium glutamicun <400> 828 Met Arg Phe Ala Ile Ile Gly Ala 1 5 Tyr Glu Ile His Lys Ala Asp Pro Ala Gly Glu Arg Ile Gly Gly Lys 40 Gly Pro Thr Asp Ile Gly Ala Glu Gly Leu Ala Gly Leu Thr Ala Ala 10 Thr Ala Gin Ile Asp Val Leu Glu 25 Leu Phe Thr Val Pro Phe Ala Ser Ala Phe Leu Ala Ala Arg Ser Asp 1146 WO 01/00843 PCTIIB0O/00923 55 Ala Val Glu Phe Phe Thr Glu Leu Gly Leu Ala Asp Ser Leu Val Ser 70 75 Pro Ser Ala Ala Lys Ser Gin Tyr Phe Ala Gly Gly Ala Leu <210> 829 <211> 1037 <212> DNA <213> Corynebacterium giutanicun <22 0> <221> CDS <222> (1)..(1014) <223> FRXA00383 <400> 829 gca Ala 1 tct Ser act Thr gat Asp cag Gin gcc Al a ccc Pro ttg Leu gcc Ala ggc Giy 145 gcg Al a ggc Giy gtg Val1 ggt Gly gc t Ala gaa Glu ttc Phe gag Glu 115 acc Thr tac Tyr c aa Gin gcc Ala tct Ser gtg Val1 gag Glu gc t Ala cag Gin 100 caa Gin aaa Lys gac Asp acc Thr gtg Val ctg Leu gc t Ala gcc ggc Ala Gly 70 cag cgg Gin Arg acc ttc Thr Phe tgc ggt Cys Gly gat ggt Asp Giy aag gtg Lys Val 150 gct Ala cgc Arg ctt Leu tcc Ser gag Giu gaa Giu aag Lys gca Ala gaa Giu 135 att Ile act cct Thr Pro ggc gat Gly Asp tat tcc Tyr Ser ctt gct Leu Ala ctg tca Leu Ser aca act Thr Thr gcg gag Ala Giu ttg gat Leu Asp atc aag Ile Lys 140 ccc gct Pro Ala 155 9gc Gly gag Giu tcC Ser gca Ala gc t Ala t ca Ser c tg Leu agt Ser 125 ggc Gly cca Pro caa Gin atc Ile acc Thr gcc Ala gcg Ala gaa Giu tac Tyr 110 ttc Phe ggt Gly acc Thr gac Asp gtc Val1 gct Ala ctt Leu gtc Val1 ac c Thr gaa Glu gtt Val1 9gc Gly gcc Ala gtg ctg ctc cgc gac ttg gca ccg gcc gca gcg cca cat ttg cgc gca 1147 WO 01/00843 Val Leu Leu att aag ttg Ile Lys Leu gag ggc ctg Glu Gly Leu 195 ggc atc acg Gly Ile Thr 210 ctg gag gct Leu Giu Ala 225 ggc gat gag Gly Asp Giu gcc ctc gac Ala Leu Asp gga ctg ggt Gly Leu Gly 275 tat gga gtt Tyr Gly Val 290 gca gcc gtg Ala Ala Val 305 gga gtt ccc 1008 Arg gc t Ala 180 ccc Pro gcg Ala cgc Arg gcg Ala gat Asp 260 gaa Glu gat Asp cct Pro gca Ala gca Ala tcc Ser t tc Phe 215 gcg Ala cgc Arg acc Thr gtg Val gct Ala 295 gaa Giu gca Ala gtc Val 185 gtc Val1 ttc Phe gtg Val gac Asp ac c Thr 265 cgc Arg gt t Val1 at t Ile gcc Ala 170 ggC Gly c tg Leu tcc Ser c gc Arg gag Glu 250 ggg Gly tgg Trp tcg Ser ggc Gly cag Gin 330 PCT/iBOO/00923 Arg Ala 175 tcc agc 576 Ser Ser gag ccg 624 Glu Pro cct cac 672 Pro His agg cta 720 Arg Leu 240 gac gcc 768 Asp Ala 255 gct gcc 816 Ala Ala cca gcc 864 Pro Ala gag atc 912 Glu Ile gga gtg 960 Gly Val 320 agg ttg Arg Leu 335 Gly Val Pro Ala Val Ilie Ala Asp Ala ctg gga 1037 Leu Gly taagcaccca aaaacactat tga <210> 830 <211> 338 <212> PRT <213> Corynebacteriun glutanicuxn <400> 830 Ala Gly Ala Gin Asp Thr Ala Phe Asp Trp Thr Pro Gly Gin Asp Ile 1 5 10 Ser Val Gly Ala Leu Val Arg Arg Gin Tyr Gly Asp Glu Ile Val Asp 25 1148 WO 01/00843 Thr Val Val PCT/1BOO/00923 Ser Ser Leu Leu Gly Gly Val Tyr Ser Ser Thr Ala Asp 40 Asp Gin Ala Pro Leu Ala Gly 145 Val Ile Glu Gly Leu 225 G ly Ala Gly Tyr Ala 305 Val Giu Ala Gin 100 Gin Lys Asp Arg Ala 180 Pro Ala Arg Ala Asp 260 Glu Asp Pro Arg Ala Gin Thr Cys Asp Lys Asp 165 Ser Asp Lys Gly Ser 245 Leu Ile His Gly Ser Glu Glu Lys Ala Glu 135 Ile Ala Ala Ser Phe 215 Ala Arg Thr Val Ala 295 Glu Ala Ser Thr Glu Asp Lys 140 Ala Pro Arg Ala Lys 220 Ser Leu Asp Gly Ala 300 Trp Ala Ala Ser Leu Ser 125 Gly Pro His Phe Val 205 Lys Phe Leu Gly G ly 285 Arg Ala Leu Val Thr Giu Val Gly Ala Arg 175 Ser Glu Pro Arg Asp 255 Ala Pro Glu Gly Asp Lys Arg Ala Ser Giu Ala 160 Ala Ser Pro His Leu 240 Ala Ala Ala Ile Val 320 Gly Val Pro Ala Val Ile Ala Asp Ala Gin Ala Ala Val His Arg Leu Leu Gly 1149 WO 01/00843 WO 0100843PCT/IBOO/00923 <210> 831 <211> 873 <212> DNA <213> Corynebacterium <220> <221> CDS <222> (101)..(850) <223> RXA01253 <400> 831 acgccatcgc agcctgccct giutanicun cctggccgca tcgaagtcct cgccaactac accgcattcc gagacctcaa aaaggctctg gagaaaggga ccgaacaata atg acc acc ctc Met Thr Thr Leu atc ggc ctc atc Ile Gly Leu Ile c tc Leu ccc gac gtc ctc Pro Asp Val Leu act tac ggc gac Thr Tyr Gly Asp gac ggc Asp Gly aac gca cta Asn Ala Leu gaa atc cag Giu Ilie Gin ctg cgc caa cgc Leu Arg Gin Arg cgc atg cgt ggc Arg Met Arg Gly att aat gct Ile Asn Ala cgc gtc acc ctc Arg Val Thr Leu gac gcc gtc cct tcc acc ctt gat Asp Ala Val Pro Ser Thr Leu Asp 259 ctc tac tgc ctc ggc ggc ggc gag gac acc gca cag Leu Tyr Cys Leu Giy Gly Giy Giu Asp Thr Ala Gin 60 atc ctt gcc acc Ile Leu Ala Thr gaa Glu cac ctc acc aaa His Leu Thr Lys ggc ggc ctc caa Gly Gly Leu Gin gca gcc gcc gca Ala Ala Ala Ala cgc ccc atc ttc Arg Pro Ilie Phe gtc tgc gca ggt ctc cag gta ctc ggc Val Cys Ala Gly Leu Gin Val Leu Gly gac tcc Asp Ser 100 ttc cgc gcc Phe Arg Ala acc acc gtc Thr Thr Val 120 ggc cgt gtc atc Gly Arg Val Ile ggc ctt ggg ctt Gly Leu Gly Leu atc gac gcc Ile Asp Ala 115 gaa acg aca Glu Thr Thr tct tta caa aaa Ser Leu Gin Lys gcc atc gga gaa Ala Ilie Gly Giu cca acc Pro Thr 135 cgc gcc gga ttc Arg Ala Gly Phe acc Thr 140 gcc gag ctg acc Ala Glu Leu Thr cga ctc acc ggc Arg Leu Thr Gly gaa aac cac atg Glu Asn His Met ggC Gly 155 gcc acc ctg ctc Ala Thr Leu Leu ccc gac gcc gaa Pro Asp Ala Glu ctc ggc cga gtc Leu Gly Arg Val cgc ggc gaa ggc Arg Gly Giu Gly acc gat gtc tgg Thr Asp Val Trp, gca gcc Ala Ala 180 1150 WO 01/00843 tcc gaa aac Ser Glu Asn PCTIB0O/00923 caa Gin aac Asn ctg Leu 230 gcc Ala agc Ser 200 caa Gin gac Asp cgc Arg acc gac gac caa cgc caa caa ttc gcc gaa Thr Asp Asp Gin Arg Gin Gin Phe Ala Giu 185 190 atc atc gcc acc tac atg cac ggc ccc gca Ile Ile Ala Thr Tyr Met His Gly Pro Ala 205 210 ctc gcc gac ctc atg ctc gca aaa gca atg Leu Ala Asp Leu Met Leu Ala Lys Ala Met 220 225 ctg gag cct ttg gac atc gac gtc atc gac Leu Glu Pro Leu Asp Ile Asp Val Ile Asp 235 240 ctg gcg tagccccttc taaaccgggt cta Leu Ala 250 gcc gtc Ala Val gcc cga Ala Arg gtc gcg Vai Ala ctc cgc Leu Arg 245 <210> 832 <211> 250 <212> PRT <213> Corynebacterium giutamicum <400> 832 Met Thr Thr Leu Asn Ile Gly Leu Ile 1 Tyr Arg Pro Gin Ala Vai Gly Glu Giu 145 Pro Gly Gly Ser Ilie Ala Leu Leu Val 130 Arg Asp Asp Ile Thr Leu Ala Gly Ilie 115 Giu Leu Ala Leu 10 Leu Val Gly Lys Ala Gly Leu Gly Met Val1 170 Pro Arg Thr Gly Asp 75 Val Arg Gin Phe Gly 155 Arg Gly Arg Ala Thr Gin Leu Gly Ile Leu Leu Asn 175 Thr Met Val1 Ala Thr Gin Leu Gly Thr Gly 160 Thr 1151 WO 01/00843 Asp Vai Trp Ala Giu Gly 195 Pro Ala Leu 210 Ala Met Giy 225 Ile Asp Arg Ser Giu Asn Thr 185 Gin Gly Ser Ilie 200 Asn Pro Gin Leu 215 Leu Lys Asp Leu 230 Ala Giu Arg Leu PCTJIBOO/00923 Asp Asp Gin Arg Gin Gin Phe 190 Ile Ala Thr Tyr Met His Giy 205 Ala Asp Leu Met Leu Ala Lys 220 Giu Pro Leu Asp Ile Asp Vai 235 240 Ala 250 <210> 833 <211> 1044 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS <222> (101)..(1021) <223> RXA02134 <400> 833 tgatgaacga catgtcgaca ttttcttccg ccggcgtcga ccgaagcgcc cgagcaaaac acggagtaac tttctaagcg tggaccccta ggC Gly ccc Pro ccc Pro atg Met atc Ile gga Gly Cgg Arg ccc Pro gaa Giu ggc Giy t tg Leu tgg Trp, gtt Val c ac His 105 gaa Giu ggt Gly gc a Al a ccc Pro gct Ala gcc Ala ctc Leu gat Asp atc Ile tca Ser ttt Phe atc Ilie 75 gtt Val gac Asp ccc Pro ttt Phe gt t Val gtt Val ggC Giy ctg Leu ggc Gly atg tcc ggc aaa gca Met Ser Gly Lys Ala 1 aac cgc cac ggg aac Asn Arg His Gly Asn tgg atg acc gtt ctc Trp Met Thr Val Leu acg ggc gcc cgg gta Thr Gly Ala Arg Val gga atg ttc acc gcg Gly Met Phe Thr Ala gtg atc cac gtc gat Val Ile His Val Asp tgg gaa ctt ctt aat Trp, Glu Leu Leu Asn 100 tcc gat gct ttg ggt Ser Asp Ala Leu Gly 115 1152 WO 01/00843 tcc tac gca Ser Tyr Ala 120 PCTAJBOO/00923 gcc cca cca cgc Ala Pro Pro Arg cga gaa atc ctt Arg Giu Ile Leu gat ccc cgc Asp Pro Arg acc gga Thr Giy 135 ctt ttc ggc ctc gcc acc gcc atg ctt Leu Phe Gly Leu Ala Thr Ala Met Leu 140 gtt ctc ctg cag Val Leu Leu Gin gtc Val1 150 gct gca gtc gca Ala Ala Val Ala tcg Ser 155 ctt gtc gat tca Leu Val Asp Ser gtg tgg tgg atg Val Trp Trp Met tgc ttc atc ccc Cys Phe Ile Pro gtt Val 170 ctc ggc cgc atc Leu Gly Arg Ile gga caa gta acc Gly Gin Val Thr gca ctg Ala Leu 180 aaa aac cac Lys Asn His gga acg gtg Gly Thr Val 200 gcc ttc tcc ccc Ala Phe Ser Pro ggc ttt ggc gca Gly Phe Gly Ala cta gtc atc Leu Val Ile 195 gtt act gct Val Thr Ala aaa ttt tgg tgg Lys Phe Trp Trp gcg ctg tgg ctc Ala Leu Trp, Leu gcg ttg Ala Leu 215 gct ttt tgg tgc Ala Phe Trp Cys gca Ala 220 gaa tta att tct Giu Leu Ile Ser ctt tca ccg ctg Leu Ser Pro Leu ac c Thr 230 agt gtt aac act Ser Val Asn Thr ccc Pro 235 ttt gtc gct gga Phe Vai Ala Giy ttc ccc gct gca Phe Pro Ala Ala aac ccc gcc tgg Asn Pro Ala Trp ct t Leu 250 gga ggc tgg gtt Gly Gly Trp Val ata acc gca gtc Ile Thr Ala Val gtg gca Val Ala 260 tgt gtt ttc Cys Vai Phe gga ctc aac Gly Leu Asn 280 gca ctt ttc agc Ala Leu Phe Ser cgc tta tca cga Arg Leu Ser Arg agt ttc ggt Ser Phe Gly 275 ggg gcg tcg Gly Ala Ser gga gac tgc atc Gly Asp Cys Ile ggc Gly 285 gca tgc att cat Ala Cys Ilie His att tct gca gtg atg ttt gct gtt gtc gcc aat gca atg gtg 1021 Ile Ser 295 Ala Val Met Phe Ala 300 Val Val Ala Asn Ala Met Val 305 taaagcggtg gcgtcttttg gga 1044 <210> 834 <211> 307 <212> PRT <213> Corynebacteriun glutanicun <400> 834 Met Ser Gly Lys Ala Gly Phe Thr 1 5 Pro Glu Asp Pro Glu Asp Ser Asp 10 1153 WO 01/00843 Asn Arg His PCT/IBOO/00923 Trp Thr Gly Val Trp Ser Leu Ser 145 Val Gin Giy Leu Pro 225 Phe Thr Ser His Ala 305 Met Thr Gly Ala Met Phe Ile His Glu Leu Asp Ala 115 Ala Asp 130 Val Leu Trp Trp Val Thr Ala Leu 195 Leu Val 210 Leu Ser Pro*Ala Ala Val Arg Ser 275 Leu Gly 290 Met Val Gly Val1 Arg Thr Val Leu 100 Leu Pro Leu Met Ala 180 Val Thr Pro Ala Val 260 Phe Ala Leu Val Al a Asp Asn Gly Arg Gin Ile 165 Leu Ile Ala Leu Ile 245 Ala Gly Ser Asn Pro Leu Phe Giu Gly Ile Phe Thr Ala Leu Asn 25 Pro Met Ilie 70 Gly Arg Ser Thr Val 150 Cys Lys Gly Al a Thr 230 Asn Cys Gly Ile Val1 Ala 55 Ile Leu Phe Tyr G ly 135 Ala Phe Asn Thr Leu 215 Ser Pro Val Leu Ser 295 Pro 40 Ser Met Leu Met Ala 120 Leu Ala Ile His Val 200 Ala Val Ala Phe Asn 280 Ala Gly Leu Trp Val His 105 Ala Phe Val1 Pro Asn 185 Lys Phe Asn Trp Ala 265 Gly Val1 Al a Pro Ala Ala Leu Pro Gly Ala Val1 170 Ala Phe Trp Thr Leu 250 Ala Asp Met Ser Phe Ile 75 Val Asp Pro Leu Ser 155 Leu Phe Trp, Cys Pro 235 Gly Leu Cys Phe Val Val1 Gly Leu Gly Arg Ala 140 Leu Gly Ser Trp, Ala 220 Phe Gly Phe Ile Ala 300 Phe Gly Pro Ile Leu Ala 125 Thr Val1 Arg Pro Ilie 205 Giu Val Trp Ser Gly 285 Val Asp Phe Ile Val Ala 110 Arg Ala Asp Ile Thr 190 Ala Leu Ala Val1 Arg 270 Ala Val1 Thr Phe Gly Phe Val Ile Leu Thr 160 Gly Phe Trp Ser Pro 240 Ile Leu Ile Asn <210> 835 <211> 1197 1154 WO 01/00843 WO 0100843PCTAIBOOIOO923 <212> DNA <213> Corynebacteriun glutainicum <220> <221> CDS <222> (101)..(117.4) <223> RXA02135 <400> 835 cgtgtcgcag cgatttgcga gagggttgtc ttcgtggttg ctggtctgcc actagagttg aaaacgtttt aagaaaacag tcggtttgaa ggagttgtta atg gtt cca gca Met Val Pro Ala 1 ctt ttt gcg cgt Leu Phe Ala Arg gaa ttt ccg gat Giu Phe Pro Asp aaa atc ctg gct Lys Ile Leu Ala cag acg Gin Thr aag gat ttc Lys Asp Phe ttg gag cag Leu Giu Gin cca cgt cca Pro Arg Pro cat His gac tcc ctc acc aag cca ccc gga tct ttg ggc aag Asp Ser Leu Thr Lys Pro Pro Gly Ser Leu Gly Lys atc ggc tgt ttc Ile Gly Cys Phe tcc gca tgc cag Ser Ala Cys Gin ggc Giy cag att ccg Gin Ile Pro ctc aac aac Leu Asn Asn aag atc gtt gtt Lys Ile Val Vai gct ggc gat cac Ala Gly Asp His gtt gca act aaa Val Ala Thr Lys gtg tcc gcg tac Vai Ser Ala Tyr tcc tca gta agc Ser Ser Vai Ser cag atg gct gaa Gin Met Ala Giu att aca aac ggt Ile Thr Asn Gly gcc gcc atc aac Ala Ala Ile Asn gtg att Val Ile 100 gca cgc acc Ala Arg Thr cac gaa gca His Giu Ala 120 acc Thr 105 ggc acg tcc gtc Gly Thr Ser Val ctt att gat acc Leu Ile Asp Thr tcc ctc gac Ser Leu Asp 115 gga tcc atc Gly Ser Ile tgg ggc gac gag Trp Gly Asp Giu gta tct agg tcc Val Ser Arg Ser tgc Cys 130 gat gtt Asp Val 135 gaa gac gcc atg acc caa gaa cag gtc Giu Asp Ala Met Thr Gin Giu Gin Val 140 cgc gca ctg aag Arg Ala Leu Lys ggt aag cgc att Gly Lys Arg Ile gat caa gaa gtg Asp Gin Glu Val gca ggc gcc gac Ala Gly Ala Asp tta atc ccc ggc Leu Ilie Pro Gly gat Asp 170 tta gga att ggc Leu Gly Ile Gly aac acc acc acc gcc gct Asn Thr Thr Thr Ala Ala 175 180 ctc gtt gga acg ttc acc ctc gca gag cct gtt gtt gtc gta ggc cgc Leu Val Gly Thr Phe Thr Leu Ala Giu Pro Val Val Val Val Gly Arg 1155 WO 01/00843 WO 0100843PCT/IBOO/00923 185 190 195 ggc acc gga atc gac gat gaa gcc tgg aaa ctc aaa gtc tcc gcg atc 739 Gly Thr Gly Ilie Asp Asp Giu Ala Trp Lys Leu Lys Val Ser Ala Ile 200 205 210 cgc gac gcc atg ttc cgc gcc cgc gac ctg cgc caa gac ccc atc gcc 787 Arg Asp Ala Met Phe Arg Ala Arg Asp Leu Arg Gin Asp Pro Ile Ala 215 220 225 atc gcc cgg aaa atc tct tcc cca gac ctt gca gcc atg gca gca ttc 835 Ile Ala Arg Lys Ilie Ser Ser Pro Asp Leu Ala Ala Met Ala Ala Phe 230 235 240 245 att gcc caa gca gca gtt cga cgc acc ccc gtg ctt ctc gac ggc gtt 883 Ile Ala Gin Ala Ala Val Arg Arg Thr Pro Val Leu Leu Asp Giy Val 250 255 260 gta gtc acc gcc gca gcc ctc cta gcc aac aaa ctg gcc cca ggt gcc 931 Val Val Thr Ala Ala Ala Leu Leu Ala Asn Lys Leu Ala Pro Giy Ala 265 270 275 agg cgt tgg ttc atc gca gga cac cgc tcc acc gaa cca gcg cat tcc 979 Arg Arg Trp Phe Ile Ala Gly His Arg Ser Thr Glu Pro Ala His Ser 280 285 290 gta gct cta aac gca ctg gcc ctt gat ccc atc ctg gaa ctt gga atg 1027 Val Ala Leu Asn Ala Leu Ala Leu Asp Pro Ile Leu Glu Leu Gly Met 295 300 305 tcc ctt ggc gaa ggc tcc ggc gca gcc acc gca ctc ccc ctg gtc aag 1075 Ser Leu Gly Glu Gly Ser Gly Ala Ala Thr Ala Leu Pro Leu Val Lys 310 315 320 325 att gcc gtt gac ctg atg aac gac atg tcg aca ttt tct tcc gcc ggc 1123 Ilie Ala Val Asp Leu Met Asn Asp Met Ser Thr Phe Ser Ser Ala Gly 330 335 340 gtc gat gga ccc cta aac gcc tct tcc gaa gcg ccc gag caa aac acg 1171 Val Asp Gly Pro Leu Asn Ala Ser Ser Glu Ala Pro Glu Gin Asn Thr 345 350 355 gag taactttcta agcgatgtcc ggc 1197 Glu <210> 836 <211> 358 <212> PRT <213> Corynebacterium giutamicun <400> 836 Met Val Pro Ala Giu Leu Phe Ala Arg Val Glu Phe Pro Asp His Lys 1 5 10 1156 WO 01/00843 Ile Leu Ala Gly Ser Leu Gin Giy Gin Phe Ala Giy Ser Ser Vai Ala Ilie Asn Asp Thr Ser 115 Ser Cys Gly 130 Giu Arg Ala 145 Ala Giy Ala Thr Thr Ala Vai Val Vai 195 Lys Vai Ser 210 Gin Asp Pro 225 Ala Met Ala Leu Leu Asp Leu Ala Pro 275 Giu Pro Ala 290 Leu Giu Leu 305 Leu Pro Leu Gin Gly Ile Asp Ser Val 100 Leu Ser Leu Asp Ala 180 Gly Ala Ile Ala G ly 260 Giy His G ly Val1 Thr Lys Pro His Leu Ile Asp Ile Lys Ile 165 Ala Arg Ile Ala Phe 245 Val Ala Ser Met Lys 325 Lys Leu Pro Gly 70 Gin Ala His Asp Ilie 150 Leu Leu Gly Arg Ile 230 Ile Val1 Arg Val Ser 310 Ile Asp Giu Arg 55 Val Met Arg Giu Val 135 Gly Ile Val Thr Asp 215 Ala Ala Val1 Arg Ala 295 Leu Ala Phe Gin 40 Pro Ala Ala Thr Ala 120 Giu Lys Pro Gly Gly 200 Ala Arg Gin Thr Trp, 280 Leu Gly Val His 25 Ile Leu Thr Giu Thr 105 Trp Asp Arg Gly Thr 185 Ile Met Lys Ala Ala 265 Phe Asn Giu Asp Asp Gly Asn Lys Asn 90 Gly Gly Ala Ile Asp 170 Phe Asp Phe Ile Ala 250 Ala Ile Ala Gly Leu 330 Ser Cys Asn Gly 75 Ile Thr Asp Met Ala 155 Leu Thr Asp Arg Ser 235 Val Ala Ala Leu Ser 315 Met Asn Leu Phe Ser Val Thr Ser Giu Thr 140 Asp Gly Leu Giu Ala 220 Ser Arg Leu Gly Ala 300 Gly Asn Ala Thr Ile Lys Ser Asn Val Arg 125 Gin Gin Ile Ala Ala 205 Arg Pro Arg Leu His 285 Leu Ala Asp Ser Lys Ser Ile Ala Gly Arg 110 Val Giu Giu Gly Giu 190 Trp Asp Asp Thr Ala 270 Arg Asp Ala Met Ser PCTIBOOIOO923 Pro Pro Ala Cys Val Val Tyr Pro Gly Ala Leu Ile Ser Arg Gin Val Val Asp 160 Asn Thr 175 Pro Val Lys Leu Leu Arg Leu Ala 240 Pro Val 255 Asn Lys Ser Thr Pro Ile Thr Ala 320 Ser Thr 335 Glu Ala Phe Ser Ser Ala Gly Val Asp Gly Pro Leu 1157 WO 01/00843 WO 0100843PCTIiBOOIO0923 340 Pro Giu Gin Asn Thr Glu 355 <210> 837 <211> 645 <212> DNA <213> Corynebacteriun glutamicum <220> <221> <222> <223>

CDS

(101)..(622) RXA02136 <400> 837 tggttttggt ctgggttact acgcgtattc ccgcaccatt cagcctcgta agtggcgtac ccctcgtgca cgcgttgaaa ttggtgctga agtctagcgc atg cgc acg tta gtt Met Arg Thr Leu Val ctt ggc ggg gcc Leu Gly Gly Ala tct ggt aag tct gct ttt gca gaa tca Ser Gly Lys Ser Ala Phe Ala Glu Ser ctt gtt Leu Val gga tct ggt Gly Ser Gly gat cct gaa Asp Pro Glu gtt ttg tat gtc gca acg gca agg cct Val Leu Tyr Val Ala Thr Ala Arg Pro tcg gga gat Ser Gly Asp cgg 6gc cca Arg Arg Pro ttc gcc gag cgc Phe Ala Glu Arg gcg gtt cat gcg Ala Val His Ala acg tct Thr Ser tgg gtg ttg gac Trp Val Leu Asp gag ggg gac gtc Glu Gly Asp Val aag ctt ctt gcc Lys Leu Leu Ala cca ccg gcc atg Pro Pro Ala Met ccg Pro gtg ctc gtt gat Val Leu Val Asp ctg ggc acc tgg Leu Gly Thr Trp acg cac gcc acc Thr His Ala Thr gcg tgc gac ggt Ala Cys Asp Gly gag gcg agt tcg Giu Ala Ser Ser gcg cag Ala Gin 100 ctt gag gcc Leu Glu Ala ggc gaa gat Gly Glu Asp 120 atg gat ttg ctt atc gac gcc atc ctc Met Asp Leu Leu Ile Asp Ala Ile Leu 110 cac ttt cag His Phe Gin 115 gga atc gtc Gly Ile Val ctg gta att gtt Leu Val Ile Val cct gaa gtt ggt Pro Glu Val Gly ccg gaa Pro Giu 135 tat aaa tct ggg Tyr Lys Ser Gly cgc Arg 140 ctt ttt cgt gat Leu Phe Arg Asp atc ggc aca ctt Ile Gly Thr Leu aat cag cgt gtc gca gcg att tgc gag agg gtt gtc ttc gtg gtt gct Asn Gin Arg Val Ala Ala Ile Cys Giu Arg Val Val Phe Val Val Ala 1158 WO 01/00843 150 155 160 ggt ctg cca cta gag ttg aaa acg ttt taagaaaaca gtcggtttga Gly Leu Pro Leu Giu Leu Lys Thr Phe 170 PCTIBOOIOO923 165 642 agg <210> 838 <211> 174 <212> PRT <213> Corynebacterium glutamicun <400> 838 Met Arg Thr Leu Val Leu Gly Gly Ala 1 Ala Arg Ala Asp Leu Ala I le Gly Arg 145 Val1 Glu Pro Glu Lys Gly Ser Leu Met 130 Ile Phe Ser Ser Arg Leu Thr Ser His 115 Gly G ly Val Leu Gly Arg Leu Trp, Ala 100 Phe Ile Thr Val1 5 Val1 Asp Pro Ala Leu Gin Gin Val Leu Ala 165 Gly Asp Thr Ser 70 Thr Leu Gly Pro Asn 150 Gly Ser Pro S er 55 Pro.

His Giu Giu Glu 135 Gin Leu Gly Glu 40 Trp, Pro Ala Ala Asp 120 Tyr Arg Pro Pro 25 Phe Val Ala Thr Lys 105 Leu Lys Val1 Leu Arg 10 Val1 Ala Leu Met Asp 90 Met Val Ser Ala Glu 170 Ser Leu Glu Asp Pro 75 Ala Asp Ile Gly Ala 155 Leu Gly Tyr Arg Glu Val Cys Leu Val Arg 140 Ile Lys Lys Val1 Ile Giu Leu Asp Leu S er 125 Leu Cys Thr Ser Ala Ala Gly Val1 Gly I le 110 Pro Phe Glu Phe Ala Thr Val Asp Asp Trp Asp Glu Arg Arg Phe Ala His Val1 Asp Giu Al a Val1 Asp Val1 160 <210> 839 <211> 575 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> <223> RXN03114 <400> 839 act ccg ggg cat ttt gtt gcg ctc gcg cgg gag att gcg ggc gcg gtg 48 1159 WO 01/00843 Thr Pro Giy 1 cgc cgc gag Arg Arg Glu agg cag agc Arg Gin Ser att cat atc Ile His Ile gta gtt gag Vai Vai Giu gaa agt gtt Giu Ser Val gag gca cag Giu Ala Gin gtg gtg tcg Val Val Ser 115 tta gcg cgt Leu Ala Arg 130 tgg aat tcg Trp Asn Ser 145 gtg gtg aag Val Val Lys ccg ctt ctg Pro Leu Leu Val1 gtg Val gtt Val1 gtg Val cg t Arg 70 t tg Leu ccl: Pro gcg Aia gcg Ala att Ile 150 gc t Ala Ala ggg Gly ggt G ly tct Ser 55 ggt Gly tgt Cys gcg Ala ggt Gly gtg Val 135 cat His ccc Pro Ala Arg 10 gat: gct Asp Ala ttlg ctl: Leu Leu ggg cag Gly Gin gct gat Ala Asp ccc gtg Pro Val altt ggg Ile Gly 105 cca ggg Pro Gly gag gcg Giu Ala ttg tat Leu Tyr ggg tltg Gly Leu 170 Giu gg t Gly gtc Val1 ltcg Ser gct Ala 75 gca Ala tgg Trp ggg Gly gag Giu gag Giu 155 gttl Val Ile Ala gac ggt Asp Giy gac gcc Asp Ala gtt gcg Val Ala tcg gag Ser Giu ccg gltt Pro Vai tltg gag Leu Giu cgg gtg Arg. Val1 125 acc act Thr Thr 140 ggt gtt Gly Val ltlt gat Phe Asp Gly ccg Pro tcc Ser ccg Pro t tg Leu tca Ser cat His 110 gag Giu altt Ile gca Ala gc l PCTIIBOO/00923 Ala Val altt tta 96 Ile Leu ttc cac 144 Phe His gat: gat 192 Asp Asp tcc gtg 240 Ser Val ltta tct 288 Leu Ser gal: ggc 336 Asp Gly gct cgt 384 Ala Arg acc cca 432 Thr Pro gaa cag 480 Glu Gin 160 aat tca 528 Ala Asn Ser tca ccg gct lttg Ser Pro Ala Leu taactcgcca lttggtgcacg tct 575 <210> 840 <211> 184 <212> PRT <213> Corynebacteriun glutanicum <400> 840 Thr Pro Gly His Phe Val Ala Leu Ala Arg Giu Ile Ala Gly Ala Val 1 5 10 Arg Arg Giu Leu Thr Val Gly Leu Asp Ala Gly Asp Gly Pro Ile Leu 25 Arg Gin Ser Phe Asp Val Gly Phe Leu Leu Val Asp Ala Ser Phe His 1160 WO 01/00843 WO 0100843PCTIBOO/00923 Ile Val1 Giu Glu Val Leu Trp 145 Val1 Pro Gly Val1 Glu Asn Gly Ile Ile Leu 165 Ser Val1 Arg 70 Leu Pro Ala Ala Ile 150 Ala Pro Gly Al a Pro Ile 105 Pro Giu Leu Gly Ala Glu Val1 Glu Val1 125 Thr Val1 Asp Pro Leu Ser His 110 Glu Ile Ala Ala Asp Ser Leu Asp Ala Thr Glu Asn 175 Asp Val Ser Gly Arg Pro Gin 160 Ser <210> 841 <211> 1200 <212> DNA <213> Corynebacterium giutarnicum <220> <221> CDS <222> (101)..(1177) <223> RXN01810 <400> 841 ccaccaccac aatgttgctc atcctcgcgg cgttcgttgt gcttcaccgt cggcaacccg actggaaaat aaggcttcac cga cgc acc ctt aca tcc gta gtc ctc gcc gct Arg Arg Thr Leu Thr Ser Val Val Leu Ala Ala 15 gcc tgc gca agc tgg gat tca cct acg gca tct Ala Cys Ala Ser Trp Asp Ser Pro Thr Ala Ser 30 att gag gag atc cag gca agc tcc acc tca aca Ile Giu Giu Ile Gin Ala Ser Ser Thr Ser Thr 45 aca ggc ttg agc atc gtg gaa gat atc ggc gat Thr Gly Leu Ser Ile Val Glu Asp Ile Gly Asp cgcaggtggc tccatgactc atg aat aac gct ttt Met Asn Asn Ala Phe 1 agc ttg gcc tta acg Ser Leu Ala Leu Thr tcc aat ggt gat ctg Ser Asn Gly Asp Leu gat ccg cgc acc ttc Asp Pro Arg Thr Phe gtg gtt ccc gta acc Val Val Pro Val Thr 115 163 211 259 307 1161 WO 01/00843 gac aac gc Asp Asn Al aac gac gt Asn Asp Va tac gga ac Tyr Gly T1~ att gtg gq Ile Val G] 12 gag gtg gt Giu Val Ve 135 aat tta cc Asn Leu H-i 150 gaa gtc at Glu Val I] tcg ccg cc Ser Pro GI gcc tcc gt Ala Ser Vz 2( tcc gtt gc Ser Val A] 215 cca gaa gz Pro Giu AE 230 gga gtg tt Gly Val P1* gaa ggc ct Giu Gly Lc ctg gca cc Leu Ala Pi 2E ttc gtg at 1027 Phe Val ME PCTIBOO/00923 tca Ser gtg Val1 tat Tyr 105 cgt Arg ac c Thr ccg Pro gat Asp cgt Arg 185 gtt Val gaa Glu ccc Pro ttc Phe ggc Gly 265 gcc Ala a tg Met cca Pro gtg Val tcc Ser act Thr act Thr act Thr cag Gin 170 tcc Ser gga Gly gtc Val ctc Leu at t Ile 250 ggc Gly aac Asn tcg Ser gc t Ala gag Giu aaa Lys gt t Val1 ggc Gly ttg Leu 155 atc Ile att Ile Ctt Leu gaa Glu aaa Lys 235 t tg Leu gtc Val gcg Ala gaa Glu cca Pro gtg Val atc Ile tcc Ser 125 cac His atc Ile gca Ala tca Ser gaa Glu 205 gcc Ala gta Val gat Asp atg Met gca Ala 285 C ta Leu gac Asp cca Pro Ctc Leu t tg Leu 130 gaa Glu atc Ile acg Thr atc Ile ctc Leu 210 gat Asp cgc Arg cgc Arg ggc Gly aat Asn 290 gg t Gly gct Ala ctg Leu gtg Val 115 gcg Ala gcg Ala ggc Gly gtg Val1 cgc Arg 195 gcg Ala gaa Giu gga Gly gat Asp atc Ile 275 cca Pro atc Ile 1162 WO 01/008' 295 ctt atg 1075 Leu Met 310 cga gta 1123 Arg Val act ggc 1171 Thr Gly ggc gag 1200 Gly Giu 43 300 gaa cgc ccc ggc att gct cz Giu Arg Pro Gly Ile Ala GJ 315 ctg gcg ctt ccc gat ggt cz Leu Ala Leu Pro Asp Gly GJ 330 gag ttg ttg ctc cgc gca tc Giu Leu Leu Leu Arg Ala S( 345 31 tagatggttg tgaaggaggt tga PCTIiBOO/00923 ~g aca acc *n Thr Thr 320 *a tca ttg *n Ser Leu 335 *c cgc gaa *r Arg Giu 305 gcc gga Ala Gly gcc ttt Ala Phe ctg tat Leu Tyr caa Gin ggt Gly gtg Val 355 <210> 842 <211> 359 <212> PRT <213> Corynebacteriun giutamicum <400> 842 Met Asn Asn Ala Phe Arg Arg Thr Leu 1 Ser Ser Asp Val Thr Leu Gly Ala Ala 145 Ser 5 Thr Leu Phe Thr Gly Leu Asn Ile Leu Arg 165 Ala Giu Leu Ala Val1 Thr Gly 120 Val1 His Ile Ser Ile Ser Ser Val Tyr 105 Arg Thr Pro Asp Thr Trp Gin Ile Pro Val Ser Thr Thr Thr Gin 170 Val Ser Ser Giu Leu Asn Thr Ser Gly 140 Val1 Arg 1163 WO 01/00843 WO 0100843PCT/EB00100923 Ala Asp Lys Val1 225 Ala Gly Lys Leu Gly 305 Ala Ala Leu Thr Ile Leu 210 Asp Arg Arg Gly Asn 290 Gly Gly Phe Tyr Met Ile Arg Thr Gly 245 Ile Asp Val1 Gly Gin 325 Gin Gly Arg 185 Val Giu Pro Phe Gly 265 Ala Met Arg Aia Leu 345 Ser Giu 205 Ala Vai Asp Met Ala 285 Leu Ala Gly Ala Ile 190 Giu Ser Phe Al a Ala 270 Leu Vai Gin Gin Ser 350 <210> 843 <2i1> 963 <212> DNA <213> Corynebacterium giutanicun <220> <221> CDS <222> <223> RXS03205 <400> 843 gat tcc ggc att ccc acg cag ttg gtg Asp Ser Gly Ile Pro Thr Gin Leu Vai 1 5 gtt cgc ggg cgc acc ttt gac cgc atc Val Arg Gly Arg Thr Phe Asp Arg Ile 25 gtg gga cca ccg gaa att ggg cat gtg Vai Gly Pro Pro Giu Ile Gly His Val 40 gag ggc agc tgg ttt gaa ccg Giu Gly Ser Trp Phe Giu Pro 10 atc gcc aac ccg ccg ttc gtg Ile Ala Asn Pro Pro Phe Val tac cgc gat tcc ggc atg gat Tyr Arg Asp Ser Gly Met Asp 48 96 144 1164 WO 01/00843 WO 0100843PCTIiBOO/00923 cta gac Leu Asp ggc gcg acc gcg Gly Ala Thr Ala gtg gtc aaa gaa Val Val Lys Glu tgc gcg cat ctc Cys Ala His Leu aac Asn cct ggt ggc acc Pro Gly Gly Thr cac ctg ctc ggc His Leu Leu Gly tgg gtg cat tcc Trp Val His Ser gat caa tcg tgg Asp Gin Ser Trp cag Gin cag cgc gtt gca Gin Arg Val Ala tgg ttg ccg gat Trp Leu Pro Asp aac ggt Asn Gly tat gtt gct Tyr Val Ala gtg ggc acg Val Gly Thr 115 gtt att gag cgc Val Ile Giu Arg gcc gtg agc ccc Ala Val Ser Pro gcg cag tat Ala Gin Tyr 110 tgg ctt agt gat gag tcc ctc gat ctg Trp Leu Ser Asp Giu Ser Leu Asp Leu cgt agc ccc gag Arg Ser Pro Giu 125 gaa aaa gcc aag Giu Lys Ala Lys gca gca Ala Ala 130 gca cgc acc acc Ala Arg Thr Thr tgg ctt aac cac Trp Leu Asn His gtt Val 145 caa ggc gtt ggt Gin Gly Val Gly ggt ttc atc gcc Gly Phe Ile Ala caa cgt ctg gag Gin Arg Leu Giu gac gag gcg gat Asp Giu Ala Asp gag Giu 165 aaa tcc gat atc Lys Ser Asp Ile gct gaa tcc atg Ala Giu Ser Met acc cag Thr Gin 175 tac ttc gag Tyr Phe Giu acc gca tgg Thr Ala Trp 195 gat Asp 180 cct ctc ggc cct Pro Leu Gly Pro att gag gag tac Ile Giu Giu Tyr ttc acc cgc Phe Thr Arg 190 agc tcc cgc Ser Ser Arg ctt cgt gaa caa Leu Arg Giu Gin cgc gat tcc att Arg Asp Ser Ile c tg Leu 205 ttc aaa Phe Lys 210 gtt cgc cct ggc Val Arg Pro Gly gcc cgg gaa. caa Ala Arg Giu Gin agc ctg gcc gat Ser Leu Ala Asp gaa gaa ggc atg Giu Giu Gly Met ttt agt cct gtc Phe Ser Pro Val ttg agg ctc acc Leu Arg Leu Thr acc gat ggt cct Thr Asp Gly Pro cgt Arg 245 tgg tcc cat gat Trp Ser His Asp gat gag cat gtg Asp Giu His Val gct tcc Ala Ser 255 atc gtc gca Ile Val Ala gaa atg tac Giu Met Tyr 275 gga Gly 260 ctt aac cca cat Leu Asn Pro His ctc ccc ttt gaa Leu Pro Phe Giu gaa atc ctg Giu Ile Leu 270 ctg cac aac Leu His Asn gcg atg gct caa Ala Met Ala Gin atc gag gga gaa Ile Giu Gly Giu tcc Ser 285 ggc gcc att gcg gcg ttg gtg gat ctc atc cgc cac gga ttg gtg ttg 1165 WO 01/00843 PCTIiBOO/00923 Gly Ala Ile Ala Ala Leu Val Asp Leu Ile Arg His Gly Leu Val Leu 290 295 300 ccc gct gat ctt ctc gat tct taaataagga ctgattgtga aagccgtttt 963 Pro Ala Asp Leu Leu Asp Ser 305 310 <210> 844 <211> 311 <212> PRT <213> Corynebacteriun glutanicun <400> 844 Asp Ser Gly Ilie Pro Thr Gin Leu Val Giu Giy Ser Trp, Phe Giu Pro 1 5 10 Val Arg Gly Arg Thr Phe Asp Arg Ilie Ile Ala Asn Pro Pro Phe Vai 25 Val Gly Pro Pro Giu Ilie Gly His Val Tyr Arg Asp Ser Gly Met Asp 40 Leu Asp Gly Ala Thr Ala Leu Val Val Lys Glu Ala Cys Ala His Leu 55 Asn Pro Gly Gly Thr Ala His Leu Leu Gly Ala Trp Val His Ser Ala 70 75 Asp Gin Ser Trp Gin Gin Arg Val Ala Giu Trp Leu Pro Asp Asn Gly 90 Tyr Val Ala Trp Val Ilie Glu Arg Asp Ala Val Ser Pro Ala Gin Tyr 100 105 110 Val Gly Thr Trp Leu Ser Asp Giu Ser Leu Asp Leu Arg Ser Pro Glu 115 120 125 Ala Ala Ala Arg Thr Thr Ala Trp Leu Asn His Phe Giu Lys Ala Lys 130 135 140 Val Gin Gly Val Gly Phe Gly Phe Ile Ala Ile Gin Arg Leu Glu Glu 145 150 155 160 Asp Giu Ala Asp Glu Lys Ser Asp Ile Leu Ala Giu Ser Met Thr Gin 165 170 175 Tyr Phe Glu Asp Pro Leu Gly Pro Giu Ile Giu Glu Tyr Phe Thr Arg 180 185 190 Thr Ala Trp Leu Arg Giu Gin Thr Arg Asp Ser Ile Leu Ser Ser Arg 195 200 205 Phe Lys Val Arg Pro Gly Val Ala Arg Giu Gin Ile Ser Leu Ala Asp 210 215 220 Ala Glu Glu Gly Met Gly Phe Ser Pro Val Thr Leu Arg Leu Thr Arg 225 230 235 240 Thr Asp Gly Pro Arg Trp Ser His Asp Val Asp Glu His Val Ala Ser 245 250 255 1166 WO 01/00843 Ile Vai Ala Giu Met Tyr 275 Gly Ala Ile 290 .PCTfEBOO/00923 Leu Asn Pro His Giy 265 Leu Pro Phe Giu Giu Ile Leu 270 Leu His Asn Ala Met Ala Gin Gly 280 Ile Giu Gly Giu Ser 285 Ala Ala Leu Asp Leu Ile Arg Gly Leu Val Leu Pro Ala Asp Leu Leu Asp Ser 305 310 <210> 845 <2i1> 956 <212> DNA <213> Corynebacterium giutamicum <220> <221> <222> <223>

CDS

(933) FRXA00306 <400> 845 gat Asp 1 tcc ggc att ccc acg cag ttg gtg gag ggc agc tgg ttt Ser Gly Ilie Pro Thr Gin Leu Val Giu Gly Ser Trp Phe gaa ccg Giu Pro gtt cgc ggg Val Arg Gly gtg gga cca Val Gly Pro acc ttt gac cgc Thr Phe Asp Arg atc gcc aac ccg Ile Ala Asn Pro ccg ttc gtg Pro Phe Val ggc atg gat Giy Met Asp ccg gaa att ggg Pro Giu Ile Giy gtg tac cgc gat Vai Tyr Arg Asp cta gac Leu Asp ggc gcg acc gcg Giy Ala Thr Ala ggt ggc acc gct Giy Gly Thr Ala ttg Leu gtg gtc aaa gaa Val Val Lys Glu tgc gcg cat ctc Cys Ala His Leu aac Asn cc t Pro cac ctg ctc ggc His Leu Leu Giy tgg gtg cat tcc Trp Val His Ser gat caa tcg tgg Asp Gin Ser Trp cag cgc gtt gca gaa tgg ttg ccg gat Gin Arg Val Ala Giu Trp Leu Pro Asp aac ggt Asn Giy tat gtt gct Tyr Vai Ala gtg ggc acg Vai Gly Thr 115 gtt att gag cgc Val Ile Giu Arg gcc gtg agc ccc gcg cag tat Ala Val Ser Pro Ala Gin Tyr 110 tgg ctt agt gat Trp Leu Ser Asp tcc ctc gat ctg Ser Leu Asp Leu agc ccc gag Ser Pro Giu gca gca Ala Ala 130 gca cgc acc acc Ala Arg Thr Thr gcg Ala 135 tgg ctt aac cac ttt gaa aaa gcc aag Trp Leu Asn His Phe Giu Lys Ala Lys 140 1167 WO 01/00843 WO 0100843PCTIIIBOO/00923 gtt caa ggc gtt ggt ttt ggt ttc atc gcc Vai Gin Gly Val Gly Phe Giy Phe Ilie Ala caa cgt ctg gag Gin Arg Leu Giu gac gag gcg gat Asp Giu Ala Asp gag Glu 165 aaa tcc gat atc Lys Ser Asp Ilie gct gaa tcc atg Ala Glu Ser Met acc cag Thr Gin 175 tac ttc gag Tyr Phe Giu acc gca tgg Thr Ala Trp 195 gat Asp 180 cct ctc ggc cct Pro Leu Gly Pro att gag gag tac Ile Giu Giu Tyr ttc acc cgc Phe Thr Arg 190 agc tcc cgc Ser Ser Arg ctt cgt gaa caa Leu Arg Glu Gin cgc gat tcc att Arg Asp Ser Ile ttc aaa Phe Lys 210 gtt cgc cct ggC Val Arg Pro Gly gcc cgg gaa caa Ala Arg Giu Gin atc Ile 220 agc ctg gcc gat Ser Leu Ala Asp gaa gaa ggc atg Giu Giu Gly Met ttt agt cct gtc Phe Ser Pro Val acg Thr 235 ttg agg ctc acc Leu Arg Leu Thr acc gat ggt cct Thr Asp Gly Pro tgg tcc cat gat Trp Ser His Asp gat gag cat gtg Asp Giu His Val gct tcc Ala Ser 255 atc gtc gca Ile Vai Ala gaa atg tac Giu Met Tyr 275 ctt aac cca cat Leu Asn Pro His gga Gly 265 ctc ccc ttt gaa Leu Pro Phe Giu gaa atc ctg Giu Ile Leu 270 ctg cac aac Leu His Asn gcg atg gct caa Ala Met Ala Gin gg t Gly 280 atc gag gga gaa Ile Giu Giy Giu ggc gcc Gly Ala 290 att gcg gcg ttg Ile Ala Ala Leu gtg Val 295 gat ctc atc cgc Asp Leu Ile Arg gga ttg gtg ttg Gly Leu Val Leu ccc Pro 305 gct gat ctt ctc Aia Asp Leu Leu gat tct Asp Ser 310 taaataagga ctgattgtga aag 956 <210> 846 <211> 311 <212> PRT <213> Corynebacterium glutainicum <400> 846 Asp Ser Gly 1 Val Arg Gly Val Gly Pro Ile Pro Thr Gin Leu Val 5 Gly Ser Trp Phe Glu Pro Arg Thr Phe Asp Arg Ile Ala Asn Pro Pro Phe Val Gly Met Asp Pro Glu Ilie Gly His Val Tyr Arg Asp Leu Asp Gly Ala Thr Ala Leu Val Val Lys Giu Ala Cys Ala His Leu 1168 WO 01/00843 Asn Pro Gly Gly Thr Ala His Leu 70 Asp Gin Ser Trp Gin Gin Arg Val Tyr Val Ala Trp Val Ile Giu Arg 100 Val Gly Thr Trp Leu Ser Asp Giu 115 120 Ala Ala Ala Arg Thr Thr Ala Trp 130 135 Val Gin Gly Val Gly Phe Gly Phe 145 150 Asp Giu Ala Asp Giu Lys Ser AspI 165 Tyr Phe Giu Asp Pro Leu Gly Pro 180 Thr Ala Trp Leu Arg Giu Gin Thr 195 200 Phe Lys Val Arg Pro Gly Val Ala 210 215 Ala Giu Giu Gly Met Gly Phe Ser 225 230 Thr Asp Gly Pro Arg Tip Ser His 245 Ile Val Ala Gly Leu Asn Pro HisC 2602 Giu Met Tyr Ala Met Ala Gin Gly 275 280 Gly Ala Ilie Ala Ala Leu Val Asp I 290 295 Pro Ala Asp Leu Leu Asp Ser 305 310 <210> 847 <211> 819 <212> DNA <213> Corynebacterium giutaxnicum <220> <221> CDS <222> (101)..(796) <223> RXCO1715 PCTILBOO/00923 eu kl a ksp LO5 ;er eu Ilie le ilu L85 krg krg ro ksp ;iy 65 Ele Ala 75 Tip Val Asp His Ile 155 Ala Glu Ser Gin Thr 235 Asp Pro Gly Val1 Pro Pro Arg 125 Glu Arg Ser Tyr Leu 205 Ser Arg His Giu Ser 285 His Asp Ala 110 Ser Lys Leu Met Phe 190 Ser Leu Leu Val1 Glu 270 Leu Ser Asn Gin Pro Ala Glu Thr 175 Thr Ser Ala Thr Ala 255 Ile His Ala Gly Tyr Glu Lys Giu 160 Gin Arg Arg Asp Arg 240 Ser Leu Asn ~eu Ile Arg His Gly Leu Val Leu 300 1169 WO 01/00843 WO 0100843PCT/IBOO/00923 <400> 847 acatgttgtt ggaacatgcc ggcagagccg acactacgat tcattcgcta aagggtctgg ccactgacac tggcaaagat ccacgaaagg aagttaccct gtg agc gag ctc Val Ser Glu Leu 1.

att aaa cag ctc Ile Lys Gin Leu aaa ctg cag cgc Lys Leu Gin Arg tct cag tgg gcg Ser Gin Trp Ala gtg ttc Val Phe cgt gct att Arg Ala Ile caa gca gcc Gin Ala Ala gga gcg ctc gat Gly Ala Leu Asp gat gat cgc aca gaa gtc act gac Asp Asp Arg Thr Giu Val Thr Asp aag ttc ttt gcc Lys Phe Phe Ala ctt gaa gca gaa Leu Giu Ala Giu aaa gtc act Lys Val Thr gtc cgt ggc.att tac aac Val Arg Gly Ile Tyr Asn tcc ggc ctg cgc Ser Gly Leu Arg gac gct gac tac Asp Ala Asp Tyr atg Met atc tgg tgg cac Ile Trp Trp His gaa gaa ttc gaa Giu Giu Phe Glu att cag aag gcc Ile Gin Lys Ala gct gat ttc cgc Ala Asp Phe Arg acc acc att ttg Thr Thr Ilie Leu cag gtt tct gag Gin Val Ser Giu gtc ttc Val Phe 100 307 355 403 451 499 tgg atc gga Trp Ilie Gly ttg cct tca Leu Pro Ser 120 gct ctc cac cgt Ala Leu His Arg tct gag ttc aac Ser Giu Phe Asn aag gct cac Lys Ala His' 115 atc act gtt Ile Thr Val ttc atc atg ggt Phe Ilie Met Gly gaa gca aag gac Giu Ala Lys Asp tgg Trp 130 tac ccg Tyr Pro 135 ttc gtg cgc agc Phe Val Arg Ser tac Tyr 140 gac tgg tac atc Asp Trp Tyr Ile gag ccc ttg aag Giu Pro Leu Lys tcc cgc att ctc Ser Arg Ile Leu cgc Arg 155 gag cac gga caa Giu His Gly Gin gct gtg gaa ttc Ala Val Giu Phe gat gtt cgt gcc Asp Val Arg Ala act gtg ccg gct Thr Val Pro Ala gca ctg ggt gac Ala Leu Gly Asp tac gaa Tyr Giu 180 tgg gtg ctg Trp Val Leu atg cac aag Met His Lys 200 ttc gag gct gat Phe Giu Ala Asp ttg cac cgc att Leu His Arg Ile gtc gat ttg Val Asp Leu 195 cgt gag gag Arg Giu Giu atg cgt tac acc Met Arg Tyr Thr gct cgc ctc cac Ala Arg Leu His gtc Val 210 ctg cca ttt att tct gga cag cgc gtc gac att gca gat ctg att aag Leu Pro Phe Ile Ser Gly Gin Arg Val Asp Ilie Ala Asp Leu Ile Lys 1170 WO 01/00843 215 220 gtt ctt cct taaaagctgc ttttctaaac gat Val Leu Pro 230 <210> 848 <211> 232 <212> PRT <213> Corynebacterium giutamicum PCTLBOO/00923 225 <400> 848 Val Ser Giu Leu Asp Ile Lys Gin Leu 1 Gin Trp Ala Thr Glu Val Giu Gly Lys Ala Asp Ala Ile Gin Lys Val Ser Giu Phe Asn Lys 115 Asp Trp Ile 130 Met Giu Pro 145 Ala Val Giu Leu Giy Asp Arg Ile Vai 195 His Vai Arg 210 Ala Asp Leu 225 <210> 849 <211> 1587 Arg Gin Vai Met 70 Ala Trp Leu Tyr Arg 150 Asp Trp Met Leu Val 230 Ala Ala Arg 55 Ile Asp Ile Pro Pro 135 Ser Val1 Val His Pro 215 Leu Ile Ala Gly Trp Phe Gly Ser 120 Phe Arg Arg Leu Lys 200 Phe Pro Asn 10 Gly Phe Tyr His Arg Ala Ile Arg Leu Asn 170 Phe Arg Ser Lys Ala Phe Asn Al a 75 Thr Leu Met Ser Arg 155 Thr Giu Tyr Gly Leu Leu Ala Ala Giu Thr His Gly Tyr 140 Giu Val Ala Thr Gin 220 Arg Asp Leu Gly Phe Leu Pro 110 Giu Trp Gly Aia Giu 190 Ala Val1 Tyr Asp Glu Leu Giu Gly Ser Ala Tyr Gin Phe 175 Leu Arg Asp Ser Arg Ala Arg Asp Gin Giu Lys Ile Ala 160 Ala His Leu I le WO 01/00843 WO 0100843PCT/EBO0100923 <212> DNA <213> Corynebacteriun glutanicum <220> <221> CDS <222> (101)..(1564) <223> RXN00420 <400> 849 attcgtgaac tcatggatct ttaggcaata aatgtgagat ttcaccacct gaaaattttc gggggtaacc tttaaaggcg tggacgattt cacgcttgtc atg aac agt tct Met Asn Ser Ser ggc acg tcc agc Gly Thr Ser Ser ggc gct tcg gcc ggt gcc cac gga gcc Gly Ala Ser Ala Gly Ala His Gly Ala ctt ccc Leu Pro cta gaa gct Leu Giu Ala aaa ctg aac ggt Lys Leu Asn Gly ggc cgc aca gcc Gly Arg Thr Ala ccc acc acc Pro Thr Thr gat gca gtc Asp Ala Val gct gag gta ctt acc acc cca Ala Giu Val Leu Thr Thr Pro cta gac atc att Leu Asp Ile Ile cgc caa Arg Gln gtc gct gaa caa Val Ala Glu Gin gac tcc aag ccg Asp Ser Lys Pro tac ctc aag cgc Tyr Leu Lys Arg ggc Gly gtg att gcc cgt.

Val Ilie Ala Arg ggC Gly atg ggt cgt tcc Met Gly Arg Ser ggt gac cca gcc Gly Asp Pro Ala aac gcc ggt ggc Asn Ala Gly Gly gtc att gac atg Val Ile Asp Met cca ctg9 aac aaa Pro Leu Asn Lys atc cac Ile His 100 tcg att gat Ser Ile Asp ctc gat cag Leu Asp Gin 120 cct gat tct gcg atc gtc gat gta gat ggc Pro Asp Ser Ala Ile Val Asp Val Asp Gly 105 110 ctc atg aag gct gcc ctg cca tat ggc ctc Leu Met Lys Ala Ala Leu Pro Tyr Gly Leu 125 130 ggc gtc acc Gly Val Thr 115 tgg gtt cct Trp Val Pro gtc ctt Val Leu 135 ccc ggc acc cgc caa gtc acc atc ggt Pro Gly Thr Arg Gin Val Thr Ile Gly 140 gca atc gga cca Ala Ile Gly Pro gac Asp 150 atc cac ggt aag Ile His Gly Lys aac Asn 155 cac cac tct gca His His Ser Ala tcc ttc ggc gac Ser Phe Gly Asp gtg gtc tcc atg Val Val Ser Met ctc ctc gtt gca Leu Leu Val Ala gac Asp 175 gga cgc atc ctg Gly Arg Ile Leu cac ctc His Leu 180 gag cca gaa ggc acc gcc gaa gac cca cag ggc gac ctg ttc tgg gca Glu Pro Glu Gly Thr Ala Giu Asp Pro Gin Gly Asp Leu Phe Trp Ala 1172 WO 01/00843 WO 0100843PCTIIBOO/00923 8CC gtt Thr Val cgc atg Arg Met 215 acc aac Thr Asn 230 cac aac His Asn cca aag Pro Lys gct. cag Ala Gin ttt aat 1027 Phe Asn 295 act ttg 1075 Thr Leu 310 atg ggt 1123 Met Gly caa cca 1171 Gin Pro ggc ttc 1219 Gly Phe ttc aag 1267 Phe Lys 375 ctc aac 1315 Leu Asn 390 ggt Gly 200 acc Thr aac Asn tac Tyr ctt Leu ctg Leu 280 gc t Ala aac Asn gca Ala ctg Leu c tg Leu 360 gac Asp gtg Val ggc atg ggc ctg 8CC ggc atc atc gtc cgt gca cgc atc Gly aag Lys ttg Leu 8CC Thr ggc Gly 265 gaa Giu cca Pro aag Lys cca Pro gat Asp 345 cag Gin atc Ile t tC Phe Met acg Thr gaa Glu tat Tyr 250 cgc Arg gaa Giu cag Gin ctg Leu gcg Ala 330 ttg Leu tac Tyr atc Ile aaa Lys Gly gaa Glu gaa Glu 235 tct Ser tcC Ser ttg Leu c tg Leu acc Thr 315 aaa Lys atc Ile cag Gin cgc Arg ctg Leu 395 Leu 8cc Thr 220 8cc Thr tc t Ser 8CC Thr gca Ala atg Met 300 ctt Leu aac Asn ggC Gly ttC Phe gat Asp 380 ttt Phe Thr 205 gcc Ala gtt Val gcg Ala atc Ile ca Pro 285 aag Lys tcc Ser cag Gin gaa Giu gtg Val1 365 atg Met ggC Gly Ile att Ile cac His 240 gat Asp ggt Gly gcc Ala gat Asp ggt Gly 320 aac Asn cgt Arg aca Thr tcc Ser aac Asn 400 Val gcg Ala 225 tcC Ser gtc Val1 tcC Ser aag Lys atc Ile 305 gtg Val1 ctc Leu ggc Gly gaa Giu ggC Gly 385 cgc Arg Arg 210 gac Asp gac Asp atc Ile c tg Leu gat Asp 290 t tc Phe gct Ala acc Thr tac Tyr gc t Ala 370 cac His gca Ala Ile cgc Arg gag Glu 245 gag Glu ctt Leu aag Lys tgg Trp gcc Ala 325 tac Tyr aag Lys cct Pro gca Ala tC Ser 405 1173 WO 01/00843 tac cca atg cca ggc tgg aac gtc tgc gtt 1363 Tyr Pro Met Pro Gly Trp Asn Val Cys Val 410 415 ggt ctg gga gct ttc ttg gac gat ctg gac 1411 Giy Leu Gly Ala Phe Leu Asp Asp Leu Asp 425 430 ggc ggc cgc ctc tac ctg gcc aag gaa tcc 1459 Gly Gly Arg Leu Tyr Leu Ala Lys Giu Ser 440 445 ttc cac gcc atg tac cca ggt atg gaa ggc 1507 Phe His Ala Met Tyr Pro Gly Met Giu Gly 455 460 gag atc gac cca acc gga gtc ttt gca tct 1555 Giu Ile Asp Pro Thr Gly Val Phe Ala Ser 470 475 gag ctt tct taagaaaggg cttgaactaa aca 1587 Giu Leu Ser <210> 850 <211> 488 <212> PRT <213> Corynebacterium glutamicum <400> 850 Met Asn Ser Ser His Gly Thr Ser Ser Ser 1 5 10 His Gly Ala Leu Pro Leu Glu Ala Gin Lys 25 Thr Ala Pro Thr Thr Ala Giu Val Leu Thr 40 Ilie Val Asp Ala Val Arg Gin Val Ala Giu 55 Asp Tyr Leu Lys Arg Gly Val Ile Ala Arg 70 Gly Asp Pro Ala Gin Asn Ala Giy Gly Leu 90 Leu Asn Lys Ile His Ser Ile Asp Pro Asp 100 105 Asp Gly Gly Vai Thr Leu Asp Gin Leu Met 115 120 cct Pro gtc Val tcc Ser 450 aag Lys tcc Ser atc Ile atg Met 435 gca Ala act Thr cgc Arg PCTIIBOO/00923 cgc cca Arg Pro 420 gaa ttc Giu Phe gag aac Giu Asn cga aat Arg Asn cga ctt Arg Leu 485 Ala Asn Pro Asn Met Ile Ala Ala Ser Gly Asp Asp Gly Asp Ile Ala 125 Ala Trp Leu Ser Arg Met Val 110 Leu Giy Gly Asp Lys Ser Gin Asp Pro Ala Arg Ile Pro Tyr Pro Val Tyr 1174 WO 01/00843 Gly Leu Trp 130 Gly Ala Ile 145 Ser Phe Gly Arg Ile Leu Asp Leu Phe 195 Val Arg Ala 210 Ala Asp Thr 225 Ser Asp Gly Val Ile Ser Ser Leu Ala 275 L~ys Asp Pro 290 Ile Phe Pro 305 Val Ala Tyr Leu Thr Gin Gly Tyr Gly 355 Giu Ala Val 370 Giy His Tyr 385 Arg Ala Pro Phe Pro Ile Arg Val Met 435 Thr Ser Ala Val1 Gly Asp His 180 Trp Arg Asp Ser Pro 260 Thr Leu Ser Tyr Phe 340 Ser Giu Ser Leu Arg 420 Giu Glu Pro Pro His 165 Leu Ala Ile Arg Giu 245 Giu Leu Lys Trp Ala 325 Tyr Lys Pro Ala Ser 405 Pro Phe Asn Leu 135 Ile Val1 Pro Vai Met 215 Asn Asn Lys Gin Asn 295 Leu Gly Pro Phe Lys 375 Asn Pro Leu Giy His Pro His Ser Giu Gly 200 Thr Asn Tyr Leu Leu 280 Ala Asn Ala Leu Leu 360 Asp Val Met Gly Arg 440 Ala Arg Asn 155 Leu Ala Gly Giu Glu 235 Ser Ser Leu Leu Thr 315 Lys Ile Gin Arg Leu 395 Trp, Leu Leu Pro Gin 140 His Leu Giu Leu Thr 220 Thr Ser Thr Ala Met 300 Leu Asn Gly Phe Asp 380 Phe Asn Asp Ala Gly PCTIIBOO/00923 Ile Gly Ala Gly 160 Asp Gly 175 Gin* Gly Ile Ile Phe Ile Phe His 240 Phe Asp 255 Arg Gly Leu Ala Pro Asp Val Gly 320 Lys Asn 335 Asn Arg Pro Thr Lys Ser Gly Asn 400 Val Asp 415 Asp Lys Ser Arg Gly Trp 1175 WO 01/00843 WO 0100843PCTIBOO/00923 Leu Lys Thr Arg Asn Giu Ile Asp Pro Thr Gly 465 470 475 Met Ser Arg Arg Leu Giu Leu Ser 485 Val Phe Ala Ser <210> <211> <212> <213> <220> <221> <222> <223> 851 563

DNA

Corynebacterium giutanicum

CDS

FRXA00420 <400> 851 tgg act t Trp Thr L 1 tg aac aag eu Asn Lys 5 ctg acc ctt tcc gca gtc ggt gtg gct Leu Thr Leu Ser Ala Val Gly Val Ala 10 tac tac Tyr Tyr gcc atg ggt Ala Met Gly cca gcg aaa aac Pro Ala Lys Asn gtg aaa aac ctc Val Lys Asn Leu acc cag ttc Thr Gin Phe tac ggc tcc Tyr Gly Ser tac caa cca ctg gat ttg atc ggc gaa tgg aac cgt Tyr Gin Pro Leu Asp Leu Ile Gly Giu Trp Asn Arg aag ggc Lys Gly ttc ctg cag tac cag ttc gtg gtc ccc Phe Leu Gin Tyr Gin Phe Val Val Pro gaa gct gtt gag Giu Ala Val Glu cc t Pro ttc aag gac atc Phe Lys Asp Ile atc Ile cgc gat atg caa Arg Asp Met Gin tcc ggc cac tac Ser Gly His Tyr 240 288 gca ctc aac gtg Ala Leu Asn Val aaa ctg ttt ggc Lys Leu Phe Gly ggc aac cgc gca Gly Asn Arg Ala cca ctg Pro Leu tcc tac cca Ser Tyr Pro cca ggt ctg Pro Gly Leu 115 cca ggc tgg aac Pro Gly Trp Asn gtc Val1 105 tgc gtt gac ttc Cys Val Asp Phe cct atc cgc Pro Ile Arg 110 gtc atg gaa Vai Met Giu gga gct ttc ttg Gly Ala Phe Leu gac Asp 120 gat ctg gac aag Asp Leu Asp Lys cgc Arg 125 ttc ggc Phe Gly 130 ggc cgc ctc tac Gly Arg Leu Tyr ctg Leu 135 gcc aag gaa tcc Ala Lys Giu Ser cgc Arg 140 acc tcc gca gag Thr Ser Ala Giu aac Asn 145 ttc cac gcc atg Phe His Ala Met cca ggt atg gaa Pro Gly Met Glu tgg ttg aag act Trp Leu Lys Thr cga Arg 160 aat gag atc gac cca acc gga gtc ttt gca tct gac atg tcc cgc cga 1176 WO 01/00843 PCTIiBOO/00923 Asn Glu Ile Asp Pro Thr Gly Val Phe Ala Ser Asp Met Ser Arg Arg 165 170 175 ctt gag ctt tct taagaaaggg cttgaactaa aca 563 Leu Glu Leu Ser 180 <210> 852 <211> 180 <212> PRT <213> Corynebacterium glutamicum <400> 852 Trp Thr Leu Asn Lys Leu Thr Leu Ser 1 Ala Tyr Lys Pro Ala Ser Pro Phe Asn 145 Asn Leu Ala Leu Leu Asp Val Met 100 Gly Arg Ala Asp Ser 180 Ala Leu Tyr Ile 70 Lys Gly Phe Tyr Tyr 150 Lys Ile Gin Arg Leu Trp Leu Leu 135 Pro Ala 10 Val1 Trp Val Gin Pro Cys Leu Giu Glu Val1 Lys Asn Pro Lys 75 Gly Val Asp Ser Gly 155 Gly Asn Arg Thr Ser Asn Asp Lys Arg 140 Trp Val Leu Gly Giu Gly Arg Phe Arg 125 Thr Leu Ala Thr Tyr Ala His Ala Pro 110 Val Ser Lys Tyr Gin Gly Val Tyr Pro Ile Met Ala Thr Tyr Phe Ser Giu Ser Leu Arg Glu Giu Arg 160 Thr Gly Val Phe Ala Ser Asp Met Ser Arg Arg 170 175 <210> 853 <211> 622 <212> DNA <213> Corynebacteriui giutamicum <22 0> <221> CDS <222> (101)..(622) <223> FRXA00426 1177 WO 01/00843 WO 0100843PCTIIBOO/00923 <400> 853 attcgtgaac tcatggatct ttaggcaata aatgtgagat ttcaccacct gaaaattttc gggggtaacc tttaaaggcg tggacgattt cacgcttgtc atg aac agt tct cac 11! Met Asn Ser Ser His 1 ggc acg tcc agc Gly Thr Ser Ser ggc gct tcg gcc ggt gcc cac gga gcc Gly Ala Ser Ala Giy Ala His Gly Ala ctt *Ccc Leu Pro cta-gaa gct Leu Giu Ala gct gag gta Ala Giu Val aaa ctg aac ggt Lys Leu Asn Gly ggc cgc aca gcc Gly Arg Thr Ala ccc acc acc Pro Thr Thr gat gca gtc Asp Ala Val ctt acc acc cca Leu Thr Thr Pro cta gac atc att Leu Asp Ile Ile cgc caa Arg Gin gtc gct gaa caa Val Ala Giu Gin aac Asn gac tcc aag ccg Asp Ser Lys Pro tac ctc aag cgc Tyr Leu Lys Arg ggc Gly gtg att gcc cgt Val Ile Ala Arg ggc Gly 75 atg ggt cgt tcc Met Gly Arg Ser tat ggt gac cca gcc caa Tyr Gly Asp Pro Ala Gin 80 aac gcc ggt ggc Asn Ala Gly Giy gtc att gac atg Vai Ile Asp Met cca ctg aac aaa Pro Leu Asn Lys atc cac Ile His 1l00 tcg att gat Ser Ile Asp ctc gat cag Leu Asp Gin 120 gtc ctt ccc Val Leu Pro 135 gat tct gcg atc gtc gat gta gat ggc Asp Ser Ala Ile Val Asp Vai Asp Gly 110 ggc gtc acc Gly Val Thr 115 tgg gtt cct Trp Val Pro ctc atg aag gct Leu Met Lys Ala ctg cca tat ggc Leu Pro Tyr Gly ggc acc cgc Gly Thr Arg caa Gin 140 gtc acc atc ggt Val Thr Ilie Giy gca atc gga cca Ala Ile Gly Pro gac Asp 150 atc cac ggt aag Ile His Gly Lys aac Asn 155 cac cac tct gca His His Ser Ala tcc ttc ggc gac Ser Phe Gly Asp gtg gtc tcc atg Val Val Ser Met ctc ctc gtt gca Leu Leu Val Ala <210> 854 <211> 174 <212> PRT <213> Corynebacteriun giutamicum <400> 854 Met Asn Ser Ser His Gly Thr Ser Ser 1 5 Ser Gly Ala Ser Ala Gly Ala 10 1178 WO 01/00843 PCTIIBOO/00923 Pro Leu Giu Ala Gin Lys Leu Asn Gly Trp Gly Arg 25 His Thr Ile Asp Giy Leu Asp Gly Gly 145 Ser Ala Leu Pro Thr Asp Ala Leu Lys Pro Ala Lys Ile 100 Gly Val 115 Trp, Val Ile Gly Gly Asp Ala Arg Gly 70 Asn Ser Leu Val1 Asp 150 Val1 Giu Gin Val Ala Ile Asp Leu 135 Ile Val1 Val 40 Val1 Ile Gly Asp Gin 120 Pro His Ser Thr Gin Giy Val1 Ser Lys Arg Asn 155 Leu Pro Asn Met Ile Ala Ala Gin 140 His Leu Asp Leu Asp Ser Giy, Arg Asp Met Ile Val 110 Ala Leu 125 Vai Thr His Ser Val Ala Asp Lys Ser Gin Asp Pro Ile Ala <210> 855 <211> 930 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(907) <223> RXN00708 <400> 855 cctgcgtatc ggctgccttt ttgaattctt ttcctcctcg taccgatccc cttccctgaa gtttcgctaa cctggcgtac cct cca att ggt Pro Pro Ile Gly gaa Giu gcg Ala tcg cat His gc t Ala acc Thr att ctt Leu gg t Gly gtg Val gtt aggcctaacc t tcaattcct atg act ctt tcc ctt Met Thr Leu Ser Leu 1 ggc gca cct ggc gtt Gly Ala Pro Gly Val cgc ctc atc gac acc Arg Leu Ile Asp Thr aag gct gtc cgc gag Lys Ala Val Arg Giu agt aag ctc cct ggc 115 163 211 259 307 1179 WO 01/00843 Ser Gly Val PCTIIBOO/00923 Pro Arg Giu Leu Ile Val Thr Ser Lys Leu Pro Gly cgC Arg ttc cat gct cgc Phe His Ala Arg cta gga cgc gtc Leu Gly Arg Val att gag gaa agt Ile Giu Giu Ser tac cgc ctc aac tta gat tac atc gat ctc Tyr Arg Leu Asn Leu Asp Tyr Ile Asp Leu 95 ctc ttg att cac Leu Leu Ile His tgg cct Trp, Pro 100 aat ccc agc Asn Pro Ser gtc cgc gat Val Arg Asp 120 gat ctc tac gtc Asp Leu Tyr Val gcg tgg gaa acg Ala Trp Giu Thr ctg att gaa Leu Ile Giu 115 aac ttc ctt Asn Phe Leu gct ggc ctg gtc Ala Gly Leu Val cac atc gga gtg His Ile Gly Vai cca aat Pro Asn 135 cac att gat cgc His Ile Asp Arg cgc cgc gaa acc Arg Arg Giu Thr ggt Gly 145 gaa ctg ccg gcc Giu Leu Pro Ala aac cag atc gag Asn Gin Ile Giu cac ccc tat ttc His Pro Tyr Phe ccg Pro 160 cag gtg gag cag Gin Val Giu Gin gta Val 165 gat ttc cac gat Asp Phe His Asp ctg ggc atc att Leu Giy Ilie Ile gag gcc tgg agc Giu Ala Trp Ser ccg ctc Pro Leu 180 agc aac ggt Ser Asn Gly gag cgc tac Giu Arg Tyr 200 gga ctc gtc gaa Gly Leu Vai Giu gag Giu 190 cca ttg ctc aag Pro Leu Leu Lys gaa atc ggc Giu Ile Gly 195 tgg cat cac Trp His His ggg gtc ggc agc Gly Vai Giy Ser ggc Gly 205 gaa atc gcc ctc Giu Ile Ala Leu gcc agg gga atc gtt ccg Ala Arg Giy Ile Vai Pro att Ile 220 cca cgc tcc acc Pro Arg Ser Thr aac Asn 225 ccg gcc agg cag Pro Ala Arg Gin cgc Arg 230 agc aac ttg gag Ser Asn Leu Giu gta aag att tcg Val Lys Ile Ser ctt Leu 240 atc gac gaa gac Ile Asp Giu Asp gtc Val 245 cag gcg -att acc Gin Ala Ile Thr ttg gcg cgc aaa Leu Ala Arg Lys aac Asn 255 ggc cgg atc aaa Gly Arg Ile Lys gat caa Asp Gin 260 gat cca gcc Asp Pro Ala tat gaa gaa ttc Tyr Giu Giu Phe tagatagtta catcaaggtt ccg <210> 856 <211> 269 <212> PRT <213> Corynebacterium giutamicum 1180 WO 01/00843 PCTIIBOO/00923 <400> 856 Met Thr Leu Ser Leu Pro Pro Ile Gly Phe Gly Thr Val His Leu Asp 1 5 10 Gly Ala Pro Gly Val Giu Ala Ile Ala Thr Ala Ile Asp Ala Gly Tyr 25 Arg Leu Ilie Asp Thr Ala Tyr Asn Tyr Giu Asn Glu Gly Thr Val Gly 40 Lys Ala Val Arg Glu Ser Gly Vai Pro Arg Giu Giu Leu Ile Val Thr 55 Ser Lys Leu Pro Gly Arg Phe His Ala Arg Asp Leu Gly Arg Val Arg 70 75 Ile Glu Glu Ser Leu Tyr Arg Leu Asn Leu Asp Tyr Ile Asp Leu Leu 90 Leu Ile His Trp Pro Asn Pro Ser Lys Asp Leu Tyr Val Giu Ala Trp 100 105 110 Giu Thr Leu Ile Giu Val Arg Asp Ala Gly Leu Val Lys His Ile Gly 115 120 125 Val Ser Asn Phe Leu Pro Asn His Ilie Asp Arg Leu Arg Arg Giu Thr 130 135 140 Gly Glu Leu Pro Ala Val Asn Gin Ile Glu Leu His Pro Tyr Phe Pro 145 150 155 160 Gin Val Glu Gin Val Asp Phe His Asp Giu Leu Gly Ile Ile Thr Glu 165 170 175 Ala Trp Ser Pro Leu Ser Asn Gly Arg Gly Leu Val Glu Glu Pro Leu 180 185 190 Leu Lys Glu Ile Gly Giu Arg Tyr Gly Val Gly Ser Gly Glu Ile Ala 195 200 205 Leu Ala Trp His His Ala Arg Gly Ile Val Pro Ile Pro Arg Ser Thr 210 215 220 Asn Pro Ala Arg Gin Arg Ser Asn Leu Giu Ala Val Lys Ile Ser Leu 225 230 235 240 Ile Asp Glu Asp Val Gin Ala Ile Thr Ala Leu Ala Arg Lys Asn Gly 245 250 255 Arg Ilie Lys Asp Gin Asp Pro Ala Val Tyr Giu Glu Phe 260 265 <210> &57 <211> 695 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> WO 01/00843 <223> FRXA00708 <400> 857 acc gtg ggc aag Thr Val Gly Lys 1 att gtt acc agt Ile Val Thr Ser cgc gtc cgc att Arg Val Arg Ile gat ctc ctc ttg Asp Leu Leu Leu gag gcg tgg gaa Glu Ala Trp Glu cac atc gga gtg His Ile Giy Val cgc gaa acc ggt Arg Giu Thr Gly 100 tat ttc ccg cag Tyr Phe Pro Gin 115 att acc gag gcc Ile Thr Giu Ala 130 gag cca ttg ctc Giu Pro Leu Leu 145 gaa atc gcc ctc Giu Ile Ala Leu cgc tcc acc aac Arg Ser Thr Asn 180 att tcg ctt atc Ilie Ser Leu Ile 195 aaa aac ggc cgg PCTIIBOO/00923 gc t Ala 5 aag

LYS

gag Giu att Ile acg Thr tc t Ser gaa Giu gtg Val tgg Trp aag Lys gc t Ala 165 ccg Pro gac Asp atc gtc Val1 ctc Leu gaa Giu cac Hius c tg Leu 70 aac Asn c tg Leu gag Giu agc Ser gaa Glu 150 tgg Trp gcc Ala gaa Giu aaa cgc Arg cct Pro agt Ser tgg Trp att Ile ttc.

Phe ccg Pro cag Gin ccg Pro 135 atc Ile cat His agg Arg gac Asp gat Asp 215 gag Giu ggC Gly c ta Leu cct Pro gaa Giu ctt Leu gcc Al a gta Vali 120 ctc Leu ggc Gly c ac His cag Gin gtc Val1 200 tcg Ser cgc Arg tac Tyr aat Asn gtC Val cca Pro gtt Val 105 gat Asp agc Ser gag Glu gcc Ala cgc Arg 185 cag Gin ggt Giy 10 t tC Phe cgC Arg ccc Pro cgc Arg aa t Asn 90 aac Asn t tc Phe aac Asn cgc Arg agg Arg 170 agc Ser gcg Ala gaa Giu cta Leu tac tac Tyr gtC Val ctg Leu cac His ggc Gly gtc Val1 agc Ser att Ile 175 gta Val1 gcg Ala ttg Leu gga Gly atc Ile gtc Val aag Lys cgc Arg

CCC

Pro atc Ile gaa Giu Gly 160 C ca Pro aag Lys cgc Arg 48 96 144 192 240 288 336 384 432 480 528 576 624 672 caa gat cca Gin Asp Pro Lys Asn Gly Arg Ile Lys 210 gcc gtc Ala Val 220 tat gaa gaa ttc Tyr Giu Giu Phe tagatagtta catcaaggtt ccg 1182 WO 01/00843 WO 0100843PCTAIBOO/00923 <210> 858 <211> 224 <212> PRT <213> Corynebacterium glutanicun <400> 858 Thr Val Gly Lys Ala Val Arg Glu Ser 1 le Arg Asp Giu His Arg Tyr Ile Giu 145 Giu Arg Ile Lys Val1 Val1 Leu Al a Ile Giu Phe Thr 130 Pro Ile Ser Ser Asn 210 Leu Glu His Leu 70 Asn Leu Glu Ser Glu 150 Trp Ala Glu Lys Pro Ser Trp Ile Phe Pro Gin Pro 135 Ilie His Arg Asp Asp 215 Gly Leu Pro Giu Leu Ala Val 120 Leu Gly His Gin Val1 200 Gin Giy 10 Phe Arg Pro Arg Asn Asn Phe Asn Arg Arg 170 Ser Ala Pro Val1 His Leu Ser Asp 75 His Gin His Gly Tyr 155 Giy Asn Ile Ala Arg Arg Leu Asp Giy Asp Giu Giu 125 Giy Val Vai Giu Ala 205 Tyr Giu Asp Asp Leu Leu Arg Leu Leu Leu G ly Pro Ala 190 Leu G lu Giu Leu Tyr Tyr Val1 Leu His Gly Val Ser Ile 175 Val Ala Glu Leu Gly Ile Val1 Lys Arg Pro Ile Giu Giy 160 Pro Lys Arg Phe Leu Leu Lys <210> 859 <211> 1038 <212> DNA <213> Corynebacteriun giutarnicum <22 0> <221> CDS <222> (101)..(1015) 1183 WO 01/00843 WO 0100843PCTIBOO/00923 <223> RXA02373 <400> 859 aaagtcatag ctcatggtaa ttcagtgtag ataggcgtac ggtgggctat ccaattcatc tcaacctaag gcgcattttg gtgcgcatca aggagaaaat atg tct gtt gtg Met Ser Val Val acc ggc cta ttc Thr Gly Leu Phe ttt Phe gga tcc ccg gag gaa gag cgg gat aag Gly Ser Pro Giu Glu Giu Arg Asp Lys 15 ttg atg Leu Met caa tct ttg Gin Ser Leu cca ttg gtc Pro Leu Val a tg Met gat cag aag aat Asp Gin Lys Asn ctt tcg aag tct Leu Ser Lys Ser gaa ggt atc Giu Gly Ile ctt ggt ttt Leu Gly Phe acc ttg aat gat Thr Leu Asn Asp aaa acc att cct Lys Thr Ile Pro ggt gtg Giy Vai ttc aag gta gat Phe Lys Val Asp gat gaa gca gag Asp Giu Ala Giu cgc Arg gta gtt acc gaa Val Val Thr Glu ctt gag gta ggt Leu Giu Vai Gly cgc cac atc gat Arg His Ile Asp act Thr s0 gct gcg att tac Aia Ala Ile Tyr aat gag gaa ggt Asn Glu Giu Giy ggc cga gct att Giy Arg Aia Ile aag tcc ggc att Lys Ser Gly Ile cct cgt Pro Arg 100 gaa gag ctg Glu Glu Leu gta gaa gct Val Glu Aia 120 att act acc aag Ile Thr Thr Lys tgg aac gat cgc cac ctg gat Trp Asn Asp Arg His Leu Asp 115 gct ttt gag gag Ala Phe Glu Glu ctg cag aag ctg Leu Gin Lys Leu ttg gat tat Leu Asp Tyr gta gat Val Asp 135 ctg tac ttg gtg Leu Tyr Leu Vai tgg ccg gca ccg aag aac gat aat tat Trp Pro Ala Pro Lys Asn Asp Asn Tyr 145 gct gca tgg aag Ala Ala Trp Lys ttg gaa aag ctc Leu Giu Lys Leu ggt Gly 160 gac cgt gct cgt Asp Arg Ala Arg atc ggt gtg tgc Ile Giy Val Cys ttc ctg cca gag Phe Leu Pro Glu cac His 175 cta gaa aag ctg Leu Glu Lys Leu ctg gca Leu Ala 180 gag gca acc Glu Ala Thr ttg cag cag Leu Gin Gin 200 gtg cct gcc att Val Pro Ala Ile cag att gag ctg Gin Ile Giu Leu cac cca gct His Pro Ala 195 ggc atc act Gly Ile Thr 691 739 cgc gat gct gtt Arg Asp Ala Val gag Glu 205 gca tct ctt gca Ala Ser Leu Ala 1184 WO 01/00843 gtg gag tcg tgg ggt cct ctg gga cag Val Giu Ser Trp Gly Pro Leu Gly Gin 215 220 gag gaa cca atc gca gct gca gcg aag Giu Giu Pro Ilie Ala Ala Ala Ala Lys 230 235 cag gtt gtt atc cgt tgg cac ctg cag Gin Val Val Ilie Arg Trp H-is Leu Gin 250 aag act gtg act aag agc cgc atg gtg Lys Thr Val Thr Lys Ser Arg Met Val 265 270 ttc gaa ctc agt gat gag gag atg gct Phe Giu Leu Ser Asp Giu Giu Met Aia 280 285 aat gat cgt ggt ggt tca cac ccg aat 1025 Asn Asp Arg Gly Gly Ser His Pro Asn 295 300 gtaaggccct gca 1038 <210> 860 <211> 305 <212> PRT <213> Corynebacteriun glutamicum <400> 860 Met Ser Vai Val Gly Thr Giy Leu Phe 1 Arg Asp Lys Leu Met Gin Ser Leu Met Lys Ser Giu Giy Ile Pro Leu Vai Thr Pro Gin Leu Gly Phe Giy Val Phe Lys 55 Arg Vai Vai Thr Giu Ala Leu Giu Vai 70 Ala Ala Ile Tyr Giy Asn Glu Giu Gly Ser Giy Ile Pro Arg Giu Giu Leu Phe 100 105 Asp Arg His Leu Asp Val Glu Aia Ala 115 120 Leu Gly Leu Asp Tyr Vai Asp Leu Tyr 130 135 ggg Giy aac Asn aac Asn 255 gaa Giu gcg Ala cg t Arg cat His 240 ggt Giy aac Asn atc Ile ttt Phe 225 gga Gly ttc gtt gtg Phe Val Val PCTJLBOOIOO923 ;c gct 787 Ly Ala :a gct 835 r-o Ala 245 :c ccc 883 'ie Pro s0 tt gat 93i "ie Asp ag cgc 979 lu Arg gac gtg ti Asp Vai P1 275 gct ctt gc Ala Leu G~ 290 tagaaataa gat ctg aac Asp Leu Asn 305 Ser Lys Asp Pro Arg Arg Thr Giu His 140 Giu Ser Ile Giu Thr Lys Asn

LYS

Pro 1185 WO 01/00843 WO 0100843PCTA~BOO/00923 Lys Asn Asp Asn Tyr Val. Ala Ala Trp Lys Gly 145 150 155 Asp Arg Ala Arg Ser Ilie Gly Val Cys Asn Phe 165 170 Giu Lys Leu Leu Ala Glu Ala Thr Thr Val Pro 180 185 Glu Leu His Pro Ala Leu Gin Gin Arg Asp Ala 195 200 Ala Ala Gly Ile Thr Val Giu Ser Trp Gly Pro 210 215 Phe Asp Leu Gly Ala Glu Glu Pro Ile Ala Ala 225 230 235 Gly Lys Thr Pro Ala Gin Val Val Ile Arg Trp 245 250 Phe Val Val Phe Pro Lys Thr Val Thr Lys Ser 260 265 Ile Asp Val Phe Asp Phe Glu Leu Ser Asp Glu 275 280 Thr Ala Leu Glu Arg Asn Asp Arg Gly Gly Ser 290 295 Asn 305 <210> 861 <211> 1683 <212> DNA <213> Corynebacteriun glutamicum <22 0> <221> CDS <222> (101)..(1660) <223> RXS00389 <400> 861 ccaccactgc gtaacctttc cgagcaagat atcgcggacc tctggcgcag gttcctaccg ccttcagttg aggtgaaagc gca ctg cat ggg tgt tca ctg att gat ggc gag Ala Leu His Gly Cys Ser Leu Ilie Asp Gly Giu 15 aat ggt gag att aca gga ttc gat ccg cgc acc Asn Gly Glu Ilie Thr Gly Phe Asp Pro Arg Thr 30 cct tcc tac tct tta gca aac agc gca cag ctg 1186 Glu Pro Ile Giu 205 Gly Ala Leu Met Met 285 Pro Lys Glu Asn 190 Ala Gin Lys Gin Val1 270 Ala Asn tgtcggattt gcttgccacc atg atc acc gca acc 115 Met Ile Thr Ala Thr tgg gtc gct gga aaa Trp, Val Ala Gly Lys aat gcg agt ctg aac Asn Ala Ser Leu Asn cgc gcc gcc aca aca WO 01/00843 WO 0100843PCTIIBOO/00923 Pro tcg Ser aga Arg ggc Gly cga Arg gca Ala gga Gly gcg Ala 150 ggc Gly gcg Ala cgg Arg gtc Val1 aat Asn 230 ctg Leu gaa Glu gat Asp Ser gcg Ala gca Ala gag Glu ctc Leu gaa Glu ccg Pro 135 g ta Val1 gat Asp cat His gga Gly tac Tyr 215 atc Ile tca Ser atg Met ttg Leu Tyr aag Lys gat Asp atc Ile aca Thr acc Thr 120 cgg Arg ttc Phe aca Thr aat Asn gcc Al a 200 ggc Gly acg Thr cag Gi r agt Ser gat Asp Ser Leu Ala Asn Ser Ala Gln Leu Arg 45 cga Arg ttc Phe gtg Val ggc Gly 105 gtg Val att Ile ggg Gly gca Ala gcg Ala 185 gtc Val cgt Arg gca Ala act Thr gcc Ala 265 gca Ala ttt Phe gat Asp 75 cgg Arg gta Val1 agc Ser ctt Leu agc Ser 155 gcg Al a cc t Pro aag Lys gtg Val ggt Gly 235 ttt Phe aac Asn agt Ser gaa Glu tcc Ser gcg Ala gcc Ala gga Gly cgc Arg 140 aac Asn ttg Leu gga G ly cat His gaa Glu 220 ttt Phe agc Ser cct Pro tcc Ser agc Ser atc Ile agc Ser cgc Arg cag Gin 125 cag Gln t tc Phe gc t Al a aca Thr gag Glu 205 att Ile acc Thr cgc Arg gtg Val ttg Leu tac Tyr gct Ala c tg Leu acc Thr 110 ttc Phe cgt Arg ccc Pro gca Ala gc t Ala 190 ttt Phe 9gc Gly ggt Gly cca Pro ttc Phe 270 gc t Ala cga Arg gac Asp gag Glu agc Ser cac His cag Gln gtc Val ggC Gly 175 gag Glu gat Asp cag Gln tca Ser gtt Val 255 gtc Val gag G lu Ala Ala Thr act cca gag Thr Pro Glu gat gcg cta Asp Ala Leu ttg gga act Leu Gly Thr 100 ctc cgc ctg Leu Arg Leu 115 cgc att gaa Arg Ile Glu 130 ttg gga cca Leu Gly Pro tct act gct Ser Thr Ala gtg gtt ttt Val Val Phe 180 ggg caa gcg Gly Gln Ala 195 gtg ttt aac Val Phe Asn 210 gct gcg gat Ala Ala Asp ggt ggt ttg Gly Gly Leu cca gtc ttt Pro Val Phe 260 ggc gcg ctg Gly Ala Leu 275 acc gct tcc Thr Ala Ser Thr gtt Val tcc Ser ac c Thr ttt Phe cga Arg gtc Val1 gg t Gly 165 aag Lys gtg Val1 ctt Leu ccg Pro gca Ala 245 gca Ala gcg Ala gtc Val1 307 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1187 WO 01/00843 WO 0100843PCTIiBOO/00923 280 acc ggc 1027 Thr Gly 295 ccg cgc 1075 Pro Arg 310 ttt aaa 1123 Phe Lys gca tgg 1171 Ala Trp atc ctc 1219 Ile Leu gtg gtg 1267 Val Val 375 cag gaa 1315 Gin Giu 390 ccg gat 1363 Pro Asp gcc acg 1411 Ala Thr atc ccc 1459 Ile Pro cca acg 1507 Pro Thr 455 ccg gcg 1555 agt tcc ggg caa Ser Ser Gly Gin ggt gtt gtt ggt Gly Val Val Gly 315 gaa acc acg ggt Glu Thr Thr Gly acc aag cct Thr Lys Pro gat gct ttt gtg gcg Asp Ala Phe Val Ala 320 caa acg atg ctc acg Gin Thr Met Leu Thr 290 ggc ctc gtt ttc atc Gly Leu Val Phe Ile 305 ctc gta gca gcc aaa Leu Val Ala Ala Lys 325 caa ggc atc gct cag Gin Gly Ile Ala Gin 340 330 cag cgc gga Gin Arg Gly gtc gac aac ctt Val Asp Asn Leu 350 345 gcc caa Ala Gin gca cag cca agt gta aaa Ala Gin Pro Ser Val Lys 355 gag aac gcg ccg ggc ccg Giu Asn Ala Pro Gly Pro ggc acc ccc gga gat Gly Thr Pro Gly Asp 365 agt gat gtg cag gcg Ser Asp Val Gin Ala gga Gly 370 gaa Glu ttg cta Leu Leu 380 gaa atc ttc ggt Giu Ile Phe Gly 395 caa ctc cac caa Gin Leu His Gin 410 gcg gca tcg ctg gtg Ala Ala Ser Leu Val 400 gta gcc aat tca ctc Val Ala Asn Ser Leu 415 cag gat gat ttc cag Gin Asp Asp Phe Gin aat aat gtg gtg ttg Asn Asn Val Val Leu 385 gtg cgt tat gat tcc Val Arg Tyr Asp Ser 405 gag gga caa tta aca Giu Gly Gin Leu Thr 420 gaa gtc tcg aaa ctt Giu Val Ser Lys Leu 435 atc cac Ile His 425 ctc ttg Leu Leu gca tcc Ala Ser 430 gag gat ctc gcg ggc Giu Asp Leu Ala Gly 440 gg t Gly acc cgt gtt ctt tac ggc ggc tgg Arg Val Leu Tyr Gly Gly Trp 450 gtt atc cat gga ggc cct tat Val Ile His Gly Gly Pro Tyr gtg gaa gtt ggg Val Glu Val Gly 460 tca aat gcg cag Ser Asn Ala Gin cac acg His Thr 465 tcg aca agt gtt gga Ser Thr Ser Val Gly 480 acc ctg gca atc Thr Leu Ala Ile 485 Pro Ala Thr 1188 WO 01/00843 WO 0100843PCTIBOO/00923 gag aga ttt atg cgc ccg gtt tct tat caa act ttc ccg gct gag ctg 1603 Glu Arg Phe Met Arg Pro Val Ser Tyr Gin Thr Phe Pro Ala Giu Leu 490 495 500 ctt cca gat cca gtt tct gag gcg aat aaa tgg gct gta cct cgg gaa, 1652.

Leu Pro Asp Pro Val Ser Glu Ala Asn Lys Trp Ala Val Pro Arg Giu 505 510 515 ata gac cgt taatagctgg tctttacatt tgc 1683 Ile Asp Arg 520 <210> 862 <211> 520 <212> PRT <213> Corynebacteriun glutanicun <400> 862 Met Ile Thr Ala Thr Ala Leu His Gly Cys Ser Leu Ile Asp Gly Giu 1 5 10 Trp Val Ala Gly Lys Asn Gly Giu Ile Thr Gly Phe Asp Pro Arg Thr 25 Asn Ala Ser Leu Asn Pro Ser Tyr Ser Leu Ala Asn Ser Ala Gln .Leu 40 Arg Ala Ala Thr Thr Ser Ala Lys Arg Ala Phe Giu Ser Tyr Arg Leu 55 Thr Thr Pro Giu Val Arg Ala Asp Phe Leu Asp Ser le Ala Asp Asn 70 75 Ile Asp Ala Leu Ser Gly Glu Ile Val Gin Arg Ala Ser Leu Giu Thr 90 Gly Leu Gly Thr Thr Arg Leu Thr Gly Giu Val Ala Arg Thr Ser Asn 100 105 110 Gin Leu Arg Leu Phe Ala Glu Thr Val Arg Ser Gly Gin Phe His Arg 115 120 125 Val Arg Ilie Giu Arg Gly Pro Arg Ile Asp Leu Arg Gin Arg Gin Val 130 135 140 Pro Leu Gly Pro Val Ala Val Phe Gly Ala Ser Asn Phe Pro Val Ala 145 150 155 160 Phe Ser Thr Ala Gly Gly Asp Thr Ala Ser Ala Leu Ala Ala Gly Cys 165 170 175 Pro Val Val Phe Lys Ala His Asn Ala His Pro Gly Thr Ala Giu Leu 180 185 190 Val Gly Gin Ala Val Arg Gly Ala Vai Giu Lys His Giu Phe Asp Ala 195 200 205 1 189 WO 01/00843 PCT/IB00/00923 Gly Val Phe Asn Leu Val Tyr Gly Arg Gly Val Glu Ile Gly Gin Glu 210 215 220 Leu Ala Ala Asp Pro Asn Ile Thr Ala Ile Gly Phe Thr Gly Ser Arg 225 230 235 240 Gin Gly Gly Leu Ala Leu Ser Gin Thr Ala Phe Ser Arg Pro Val Pro 245 250 255 Val Pro Val Phe Ala Glu Met Ser Ala Thr Asn Pro Val Phe Val Phe 260 265 270 Pro Gly Ala Leu Ala Asp Leu Asp Ala Ser Ser Ser Leu Ala Glu Ala 275 280 285 Phe Thr Ala Ser Val Thr Gly Ser Ser Gly Gin Leu Cys Thr Lys Pro 290 295 300 Gly Leu Val Phe Ile Pro Arg Gly Val Val Gly Asp Ala Phe Val Ala 305 310 315 320 Leu Val Ala Ala Lys Phe Lys Glu Thr Thr Gly Gin Thr Met Leu Thr 325 330 335 Gin Gly Ile Ala Gin Ala Trp Gin Arg Gly Val Asp Asn Leu Ala Ala 340 345 350 Gin Pro Ser Val Lys Ile Leu Ala Gin Gly Thr Pro Gly Asp Gly Glu 355 360 365 Asn Ala Pro Gly Pro Val Val Phe Glu Ser Asp Val Gin Ala Leu Leu 370 375 380 Asn Asn Val Val Leu Gin Glu Glu Ile Phe Gly Ala Ala Ser Leu Val 385 390 395 400 Val Arg Tyr Asp Ser Pro Asp Gin Leu His Gin Val Ala Asn Ser Leu 405 410 415 Glu Gly Gin Leu Thr Ala Thr Ile His Ala Ser Gin Asp Asp Phe Gin 420 425 430 Glu Val Ser Lys Leu Ile Pro Leu Leu Glu Asp Leu Ala Gly Arg Val 435 440 445 Leu Tyr Gly Gly Trp Pro Thr Gly Val Glu Val Gly His Thr Val Ile 450 455 460 His Gly Gly Pro Tyr Pro Ala Thr Ser Asn Ala Gin Ser Thr Ser Val 465 470 475 480 Gly Thr Leu Ala Ile Glu Arg Phe Met Arg Pro Val Ser Tyr Gin Thr 485 490 495 Phe Pro Ala Glu Leu Leu Pro Asp Pro Val Ser Glu Ala Asn Lys Trp 500 505 510 Ala Val Pro Arg Glu Ile Asp Arg 515 520 1190 WO 01/00843 WO 0100843PCT/IBOO/00923 <210> <211> <212> <213> <220> <221> <222> <223> 863 882

DNA

Corynebacteriun glutamicum

CDS

(101)..(859) RXS0 0419 <400> 863 gctggttgaa gactcgaaat gagatcgacc caaccggagt ctzttgcatct gacatgtccc gccgacttga gctttcttaa gaaagggctt gaactaaaca atg ctt aac gca gtg Met Leu Asn Ala Val ggc aaa gcc caa Gly Lys Ala Gin att ctc ctt ctt Ile Leu Leu Leu gga acc tct gag Gly Thr Ser Giu atc ggt Ile Gly att tcc att Ile Ser Ilie ttg gca gcg Leu Ala Ala tcc cgc ttc ctc Ser Arg Phe Leu cag ggt cca tcc Gin Gly Pro Ser cat gtg acc His Val Thr gtc gca gag Val Ala Giu cgt aaa gat tcc cca cgc gtg gac gca Arg Lys Asp Ser Pro Arg Val Asp Ala gca Ala atc aaa Ilie Lys gca gct ggc gct A la Ala Gly Ala tcc gtt gct gtt Ser Val Ala Val gat ttc gat gcg Asp Phe Asp Ala c tc Leu gac acc gaa tcc cac cct gca gcc atc Asp Thr Giu Ser His Pro Ala Ala Ile 75 gca gcc ttt gaa aac Ala Ala Phe Giu Asn ggc gac gtt gac Gly Asp Val Asp gta Val1 gca atc gtg gct Ala Ile Val Ala ggc atc ctc ggc Gly Ile Leu Gly gac aac Asp Asn 100 gaa gca cag Giu Ala Gin aac tac acc Asn Tyr Thr 120 cgc gac caa gca cta gca gtg gaa gca Arg Asp Gin Ala Leu Ala Val Giu Ala 110 acc acc gtg Thr Thr Val 115 cag aaa ttt Gin Lys Phe gcc ggc gtt tcc Ala Gly Val Ser gta Val 125 ggt gta ctg ctg Gly Val Leu Leu ggC Gly 130 gag cag Giu Gin 135 cag ggc cac ggc acc atc gtg gca ttg Gin Gly His Gly Thr Ile Val Ala Leu tct gtg gca ggc Ser Val Ala Gly cag Gin 150 cga gtc cgc cgc tcc aac ttt gtc tac Arg Val Arg Arg Ser Asn Phe Vai Tyr 155 tcc gcc aag gca Ser Ala Lys Ala ttc gac ggt ttc Phe Asp Gly Phe tac Tyr 170 acc cag ctc ggc Thr Gin Leu Gly gcc ctg cgt gga Ala Leu Arg Gly tcc ggt Ser Gly 180 1191 WO 01/00843 WO 0100843PCT/IBOO/00923 gcc aac Ala Asn gca gat Ala Asp gat gct Asp Ala 215 cac cca His Pro gta Val gg t Giy 200 gtt Val1 ctg Leu gtt Val gcc Ala gca Ala tac Tyr 235 ctg Leu cgc cca Arg Pro cca ctg Pro Leu 205 gtg gtg Val Val 220 gtc tct Val Ser ccg ttc Pro Phe ggc cag gta cgc acc aag atg Gly Gin Val Arg Thr LYS Met 190 195 acc gtc aac cgc gaa gac gtg Thr Val Asn Arg Glu Asp Val 210 aac aag aag gac atc atc ttt Asn Lys Lys Asp Ile Ile Phe 225 ttt gcg ttc caa ttc att. ccg Phe Ala Phe Gln Phe Ile Pro 240 taacggaagt tacggaagtt acg 691 739 787 835 230 gca Ala atc ttc cgc aag Ile Phe Arg Lys <210> 864 <211> 253 <212> PRT <213> Corynebacterium giutamicum <400> 864 Met Leu Asn Ala Vai Giy Lys Ala Gin 1 5 Thr Ser Giu Ile Gly Ile Ser Ile Val Pro Ser His Vai Thr Leu Ala Ala Arg 40 Ala Ala Val Ala Giu Ilie Lys Ala Ala Val Asp Phe Asp Ala Leu Asp Thr Giu 70 Ala Ala Phe Giu Asn Giy Asp Val Asp Ile Leu Gly Asp Asn Giu Ala Gin Trp 100 105 Giu Ala Thr Thr Val Asn Tyr Thr Ala 115 120 Leu Gly Gin Lys Phe Giu Gin Gin Gly 130 135 Ser Ser Vai Ala Gly Gin Arg Val Arg 145 150 Ser Ala Lys Ala Gly Phe Asp Gly Phe 165 Asn Ser Lys Gly Ser Val1 Arg Gly His Arg Tyr 170 Leu Leu Pro Ser Ala Val Ala Val 125 Ile Phe Leu Gly Gly Asp Val1 Asp Gly Val1 Leu Leu Gly 160 Ala 1192 WO 01/00843 Leu Arg Gl Arg Thr Ly 19 Arg Giu As 210 Asp Ilie Il 225 Gin Phe Ii Gly Ser Ala Val1 Arg 245 Ala Asn Val Leu 185 Ala Asp Gly Gly 200 Asp Ala Val Tyr 215 His Pro Leu Phe 230 Ala Ile Phe Arg PCTJIBOOIOO923 Val Val Arg Pro Gly Gin Val 190 Giu Aia Pro Leu Thr Vai Asn 205 Asp Ala Val Val Asn Lys Lys 220 Gin Tyr Vai Ser Phe Ala Phe 235 240 Lys Leu Pro Phe 250 <210> 865 <211> 1673 <212> DNA <213> Corynebacterium glutamTicum <220> <221> CDS <222> (1650) <223> RXCO0416 <400> 865 ctg gcg t( Leu Ala S4 1 att ccg cl Ile Pro Lo cag ggc ti Gin Giy P) ttg tcg gi Leu Ser A., tgg ttc t5 Trp Phe n tgg gaa g( Trp Giu A: tct gtg ti Ser Val Lo gca aca g5 Ala Thr G' tcc gaa gi Ser Giu G'.

tta Leu gcg Ala act Thr aac Asn gg t Gly cag Gin cca Pro gcg Aia tac Tyr agc Ser acc Thr c ag Gin tac Tyr ggt Gly cca Pro gtg Val1 ttg Leu gc t Ala gcg Ala atc Ile gac Asp tac Tyr ggg Gly gca Ala tcC Ser t tg Leu 125 att Ile gtg Val agc Ser gat Asp cct Pro ctg Leu gtt Val1 ctg Leu 110 gcg Aia cca Pro 1193 WO 01/00843 PCT/LBOO/00923 130 135 140 ctc gtg ctg gct tcc cgc att gcc aag ggc gat aag ttt gcg ctt gcc 480 Leu Val Leu Ala Ser Arg Ile Ala Lys Gly Asp Lys Phe Ala Leu Ala 145 150 155 160 ggc ggc att att tac ttg ggt gtt tcg gct act ttc tat act ttg ttc 528 Gly Gly Ile Ile Tyr Leu Giy Val Ser Ala Thr Phe Tyr Thr Leu Phe 165 170 175 acc ggt gct atc gcg ctt tct gcg gtc gcg gtg tgc atc gtg gtg gcg 576 Thr Gly Ala Ile Ala Leu Ser Ala Val Ala Val Cys Ile Val Vai Ala 180 185 190 gct att gtg cag cgc tcc atc aaa cca ctg ctg tgg ctt gca gtg ctg 624 Ala Ile Val Gin Arg Ser Ile Lys Pro Leu Leu Trp Leu Ala Vai Leu 195 200 205 ggt ggt gga tcc att gtc att gcg ttg att tct tgg ggt cct tac ctt 672 Gly Gly Gly Ser Ile Vai Ile Ala Leu Ile Ser Trp Gly Pro Tyr Leu 210 215 220 ctg gcc tcc atc aac gga gcg gag cgc tct ggc gat tcc gca aca cac 720 Leu Ala Ser Ile Asn Giy Ala Giu Arg Ser Gly Asp Ser Ala Thr His 225 230 235 240 tac ctg cct ctt gaa ggc acc caa ttc ccg gtt cct ttc ttg gca tca 768 Tyr Leu Pro Leu Giu Giy Thr Gin Phe Pro Val Pro Phe Leu Ala Ser 245 250 255 agc gtt gtg gga ctg ttg tgt ctt gtt ggc ctg atc tat ttg gtg gtg 816 Ser Vai Vai Gly Leu Leu Cys Leu Val Gly Leu Ile Tyr Leu Val-Val 260 265 270 cgt ttc cac aac aat gag gtg cgc gcg atg tgg gtc ggc atc gca gtg 864 Arg Phe His Asn Asn Giu Vai Arg Ala Met Trp Val Gly Ile Ala Val 275 280 285 ttt tat gcc tgg atg ggc atg tcc atg gcg atc acg ctt ttg ggc aac 912 Phe Tyr Ala Trp Met Gly Met Ser Met Ala Ile Thr Leu Leu Gly Asn 290 295 300 acg ttg ctt gga ttc cgt ctt gat acg gtg ctg gtg ctt att ttt gcc 960 Thr Leu Leu Gly Phe Arg Leu Asp Thr Val Leu Val Leu Ile Phe Ala 305 310 315 320 acg gct gga gtg ttg ggc att gca gat ttc cgc ctt gcc agt gtg tat 1008 Thr Ala Gly Vai Leu Gly Ile Ala Asp Phe Arg Leu Ala Ser Val Tyr 325 330 335 cag ctc tac ccc acc caa atc aca gag cgc acg gcc acc cat ctg acc 1056 Gin Leu Tyr Pro Thr Gin Ile Thr Giu Arg Thr Ala Thr His Leu Thr 340 345 350 aat cta att gtg gtc ctc gtg ctg ctt ggc ggc ctc tac tac gcg caa 1104 Asn Leu Ile Val Val Leu Val Leu Leu Gly Gly Leu Tyr Tyr Ala Gin 355 360 365 1194 WO 01/00843 WO 0100843PCT/IBOO/00923 gat ctg 1152 Asp Leu 370 act gat 1200 Thr Asp 385 cgt tat 1248 Arg Tyr ccg cag aag aac gca Pro Gin Lys Asn Ala 375 ggc tac ggc gag cgc Gly Tyr Gly Giu Arg cga gct atc gat ctg gcc tat acc gat Arg Ala Ile Asp Leu Ala Tyr Thr Asp 380 tac Tyr aag gac Lys Asp 405 act gtc Thr Val 420 390 atc Ile gcg gat ctg tat Ala Asp Leu Tyr 395 gat cat ctg ctt Asp His Leu Leu ccg gcc gga gct gca Pro Ala Giy Ala Ala 400 gat caa gga ttc gag Asp Gin Gly Phe Giu 415 aac Asn 410 cct tcc gaa 1296 Pro Ser Giu tac cct tat 1344 Tyr Pro Tyr 435 ctt ggt gag 1392 Leu Gly Giu 450 cga agc tgg 1440 Arg Ser Trp 465 aac acc tct 1488 Asn Thr Ser atc gat gat 1536 Ile Asp Asp tac ccg aac 1584 Tyr Pro Asn 515 gag tca ttt 1632 Giu Ser Phe 530 gtg gta acg 1673 Vai Val Thr 545 gtg ctg aca gac Val Leu Thr Asp 425 cgc gga tac caa gct Arg Giy Tyr Gin Ala 440 ttc gga aac agg aac Phe Gly Asn Arg Asn 455 gat gag ttg gct gat Asp Giu Leu Ala Asp tt t Phe gca Ala gaa ctc gat ttc atg tcc tac Giu Leu Asp Phe Met Ser Tyr 430 act tcc cac tac gcc aac ccg Thr Ser His Tyr Ala Asn Pro 445 ttc atc gaa gat ctc gcg atc Phe Ile Giu Asp Leu Ala Ile 460 cct caa caa ttq Pro Gin Gin Phe agc gac gcc ttg Ser Asp Ala Leu 480 470 475 cca tgg Pro Trp 485 acg Thr atc cct gag Ile Pro Glu gtg ttc atc ttc cgt ggc tcc Vai Phe Ile Phe Arg Gly Ser 490 495 tac gac gtg gct gaa gat ctg Tyr Asp Val Ala Giu Asp Leu cct gac gcc ggt tgg aaa Pro Asp Ala Giy Trp Lys 500 505 510 aat cca aac gtg cgc ttc cgc ggc gtg tac ttt aac ccg Asn Pro Asn Val Arg Phe Arg Gly Val Tyr Phe Asn Pro 520 525 gat cag atg tgg cag acc aag caa gtg gga cct ttc gtg Asp Gin Met Trp, Gin Thr Lys Gln Vai Gly Pro Phe Val cac aat gag His Asn Giu 550 taattcctca ccaaacgacc caa 1195 WO 01/00843 <210> 866 <211> 550 <212> PRT <213> Corynebacterium giutanicui <400> 866 Leu Ala Ser Tyr Leu Ser Pro Thr Ala PCTIIBOO/00923 1 Ile Gin Leu Trp Trp Ser Ala Ser Leu 145 Gly Thr Ala Gly Leu 225 Tyr Ser Arg Phe Leu Phe Asp Trp Ala Leu Giy 115 Giu Leu Ile Ala Val 195 Gly Ser Pro Val1 His 275 Ala Ser Arg Met Leu Phe Val1 100 Ile Pro Ala Ile Ile 180 Gin Ser Ile Leu Gly 260 Asn Trp 5 Ala Thr Asn Gly Gin Pro Ala Tyr Ser Tyr 165 Ala Arg Ilie Asn Glu 245 Leu Asn Met Thr Gin Tyr Gly 70 Pro Val Leu Ala Arg 150 Leu Leu Ser Val Gly 230 C ly Leu Glu Gly Tyr 25 Thr Met Ala Ile Arg 105 Thr Val Lys Ser Val 185 Pro Leu Arg Phe Val 265 Ala Met Leu 10 Leu Arg Pro Asn Val1 90 Ilie Cys Ala Gly Ala 170 Ala Leu Ile Ser Pro 250 Gly Met Ala Val Asp Met Thr Leu 75 Ser Thr Ilie Met Asp 155 Thr Val Leu Ser Gly 235 Val Leu Trp Ile Val Gly Ala Phe Leu Met Gly Ile Gly 140 Lys Phe Cys Trp Trp 220 Asp Pro Ile Val Thr Ala Ile Asp Tyr Gly Ala Ser Leu 125 Ile Phe Tyr Ile Leu 205 Gly Ser Phe Tyr Gly 285 Leu Val Ser Asp Pro Leu Val Leu 110 Ala Pro Al a Thr Val 190 Ala Pro Ala Leu Leu 270 Ile Leu Leu Val Ile Ala Pro Ala Pro Met Ala Leu Leu 175 Val Val1 Tyr Thr Ala 255 Val Ala Gly Ala Asp Gly Gly Gly Ala Val1 Asn Met Ala 160 Phe Ala Leu Leu His 240 Ser Val1 Val Asn 1196 WO 01/00843 PCTJIIBOO/00923 290 295 300 Thr Leu Leu Gly Phe Arg Leu Asp Thr Val Leu Val Leu Ile Phe Ala 305 310 315 320 Thr Ala Giy Vai Leu Giy Ile Ala Asp Phe Arg Leu Ala Ser Val Tyr 325 330 335 Gin Leu Tyr Pro Thr Gin Ile Thr Glu Arg Thr Ala Thr His Leu Thr 340 345 350 Asn Leu Ilie Val Val Leu Val Leu Leu Giy Gly Leu Tyr Tyr Ala Gin 355 360 365 Asp Leu Pro Gin Lys Asn Ala Arg Ala Ile Asp Leu Ala Tyr Thr Asp 370 375 380 Thr Asp Giy Tyr Gly Giu Arg Ala Asp Leu Tyr Pro Ala Giy Ala Ala 385 390 395 400 Arg Tyr Tyr Lys Asp Ile Asn Asp His Leu Leu Asp Gin Gly Phe Glu 405 410 415 Pro Ser Giu Thr Val Val Leu Thr Asp Glu Leu Asp Phe Met Ser Tyr 420 425 430 Tyr Pro Tyr Arg Giy Tyr Gin Ala Phe Thr Ser His Tyr Ala Asn Pro 435 440 445 Leu Giy Giu Phe Gly Asn Arg Asn Ala Phe Ile Giu Asp Leu Ala Ile 450 455 460 Arg Ser Trp Asp Giu Leu Ala Asp Pro Gin Gin Phe Ser Asp Ala Leu 465 470 475 480 Asn Thr Ser Pro Trp Thr Ile Pro Giu Vai Phe le.Phe Arg Gly Ser 485 490 495 Ile Asp Asp Pro Asp Ala Gly Trp Lys Tyr Asp Val Ala-Giu Asp Leu 500 505 510 Tyr Pro Asn Asn Pro Asn Val Arg Phe Arg Gly Val Tyr Phe Asn Pro 515 520 525 Giu Ser Phe Asp Gin Met Trp Gin Thr Lys Gin Val Gly Pro Phe Val 530 535 540 Val Vai Thr His Asn Glu 545 550 <210> 867 <211> 1059 <212> DNA <213> Corynebacteriun glutanicun <220> <221> CDS <222> (101)..(1036) <223> RXC02206 1197 WO 01/00843 WO 0100843PCT/EBOO/00923 <400> 867 ggcaggatct gctgctgcgg ctaggagggt tatctcttca ttcacccgat ctaccgtact accttatgac ctcagtagtg tggtgggcgt gaaacagcga atg gtc ggt rca agt Met Val Gly Ser Ser ggt ttg cgg gta Gly Leu Arg Val agg ctc ggt ttg Arg Leu Gly Leu acc tca aca tgg Thr Ser Thr Trp ggc tcg Gly Ser ggc acc gag Gly Thr Giu gct gag gca ggc Ala Giu Ala Gly arc ttr aag gcg Ile Phe Lys Ala ttc atc aat Phe Ile Asn acc ggc gtc Thr Gly Val tcr ggt ggc acg ctt atc gac gtc tcc ccc aac rac Ser Gly Gly Thr Leu Ilie Asp Vai Ser Pro Asn Tyr gcg gaa Ala Giu gaa atg ctc ggc Giu Met Leu Gly acg Thr atg ttg gat gcg Met Leu Asp Ala gtc rct cgt rcg Val Ser Arg Ser gc r Ala gtc gtc att tcc Val Val Ile Ser agc gca ggt gtc Ser Ala Gly Val ccc gct ctg ccg Pro Ala Leu Pro ggc cga cgt gtg Gly Arg Arg Val tgc tcc cgc cgc aat ttg att gcc caa Cys Ser Arg Arg Asn Leu Ile Ala Gin tta gat Leu Asp 100 gtc acc ctg Val Thr Leu gca tta aac act Ala Leu Asn Thr tat rtg gat ttg Tyr Leu Asp Leu tgg tct gtg Trp, Ser Val 115 gat act ttg Asp Thr Leu ggc tat tgg gat gag ggc acc cca ccg cat gag gtg gcc Gly Tyr Trp Asp Giu Gly Thr Pro Pro His Giu Vai Ala 120 125 130 gat rac Asp Tyr 135 gcc gtg cgc acc Ala Val Arg Thr ggc Gly 140 cga gtc cga tat Arg Val Arg Tyr ggt gtc cga gga Gly Val Arg Giy tat Tyr 150 tcc ggt tgg cag Ser Gly Tip Gin gcg gtc acc cac Ala Val Thr His gca tcc aat cat Ala Ser Asn His gcg gcc tcc gcc Ala Ala Ser Ala ccc gtg gtc gtt Pro Val Val Val caa aat gaa rac.

Gin Asn Giu Tyr agc ctg Ser Leu 180 ctg gaa cgc Leu Giu Arg ggt gtc gga Gly Val Gly 200 gca gaa caa gaa Ala Glu Gin Glu ctc cct gcc acc Leu Pro Ala Thr caa cac cta Gin His Leu 195 gtg ctg act Val Leu Thr ttc ttt gct ggc Phe Phe Ala Gly ccg ctg ggg caa Pro Leu Giy Gin gct aaa tac cgc tcc gaa att ccc cat gat tcc aga gct gca tcc aca Ala Lys Tyr Arg Ser Giu Ile Pro His Asp Ser Arg Ala Ala Ser Thr 1198 WO 01/00843 215 220 gga cgc gac gca gaa gtc caa agc tac cta Gly Arg Asp Ala Giu Val Gin Ser Tyr Leu 230 235 att gtc gat gct ctt gat act gca gcc aaa Ilie Val Asp Ala Leu Asp Thr Ala Ala Lys 250 255 gct gtc aca gcc acc acc tgg gtg cgt gat Ala Val Thr Ala Thr Thr Trp Val Arg Asp 265 270 gtc atc gtg ggc gct cgc aca cat gaa cag Val Ile Val Gly Ala Arg Thr His Glu Gin 280 285 gcg gaa tcg gtg act ttg cca aca cca atc 1027 Ala Giu Ser Val Thr Leu Pro Thr Pro Ile 295 300 gtc tcc ctg tgacttggtc caattacatt cac 1059 Val Ser Leu 310 <210> 868 <211> 312 <212> PRT <213> Corynebacterium giutamicun <400> 868 Met Val Gly Ser Ser Gly Leu Arg Val Ser 1 5 Ser Thr Trp Gly Ser Gly Thr Giu Leu Ala 25 Lys Ala Phe Ilie Asn Ser Gly Gly Thr Leu 40 Tyr Thr Thr Gly Val Ala Glu Glu Met Leu 55 Glu Val Ser Arg Ser Ala Val Val Ile Ser Pro Ala Leu Pro Leu Gly Arg Arg Val Asp Ile Ala Gin Leu Asp Val Thr Leu Arg Ala 100 105 Asp Leu Trp, Ser Val Gly Tyr Trp Asp Glu 115 120 Val Ala Asp Thr Leu Asp Tyr Ala Val Arg 130 135 PCT/IIBOO/00923 225 aat Asn t ta Leu ccc Pro tca Ser caa Gin 305 Leu Ala Asp Thr Ser Ser Asn Thr Gly 140 cga Arg ggc Gly gga Gly cat His 290 gcc Ala Gly Gly Val Met Ala Arg Thr Pro 125 Arg ggc Gly at t Ile gtg Val1 275 ctt Leu ctt Leu Leu Asp Ser Leu Gly Arg Asp 110 Pro Val 1199 WO 01/00843 WO 0100843PCT[IBOO/00923 Ala Gly Val Arg Gly Tyr Ser Gly Trp Gin Leu 145 150 155 Ala Ser Asn His Ala Ala Ala Ser Ala Arg Pro 165 170 Asn Giu Tyr Ser Leu Leu Glu Arg Arg Ala Giu 180 185 Ala Thr Gin His Leu Gly Val Gly Phe Phe Ala 195 200 Gin Gly Val Leu Thr Ala Lys Tyr Arg Ser Giu 210 215 Arg Ala Ala Ser Thr Gly Arg Asp Ala Glu Val 225 230 235 *Asn Arg Gly Arg Ile Ilie Val Asp Ala Leu Asp 245 250 Leu Gly Ile Ser Pro Ala Val Thr Ala Thr Thr 260 265 Pro Gly Val Thr Ala Val Ile Val Gly Ala Arg 275 280 Ser His Leu Leu Lys Ala Giu Ser Val Thr Leu 290 295 Gin Ala Leu Asp Asp Val Ser Leu 305 310 <210> 869 <211> 621 <212> DNA <213> Corynebacterium giutamicum <22 0> <221> CDS <222> (101)..(598) <223> RXS03074 <400> 869 tttgtgggca atctggtttt ttcgtaattg tgtgggatga tagcaggaca agcatactgt tttagttcta tgctgtgggc cca gaa ttc att gcc acc gca gac ctc gta gac Pro Giu Phe Ile Ala Thr Ala Asp Leu Vai Asp 15 gcg caa tca tgc gac act cag ttt caa aac ctt Ala Gin Ser Cys Asp Thr Gin Phe Gin Asn Leu 30 ttc cac gga ata ata acc acc gtg aaa tgc ttc Ala Vali Gin Gly Ile 220 Gin Thr Trp Thr Pro 300 atctcttaaa aattcacatt atg act caa agt gct Met Thr Gin Ser Ala 1 atc atc ggc gac aac Ile Ilie Gly Asp Asn gga ggt gcc aca gaa Gly Giy Ala Thr Giu caa gac aac gcc ctc 1200 WO 01/00843 Phe His Gly Ile Ilie Thr ctg aaa tcc atc ctg agc Leu Lys Ser Ile Leu Ser gat ggc gac gca tcc gtg Asp Gly Asp Ala Ser Vai 75 gga ctt gga aaa gat cat Gly Leu Gly Lys Asp His att cga gac tcc gca gtc Ile Arg Asp Ser Ala Val 105 ctt gga acc aac ccg cgg Leu Gly Thr Asn Pro Arg 120 gac gta gtg gta tcg att Asp Vai Val Val Ser Ile 135 gtc tac gcg gac tct gac Val Tyr Ala Asp Ser Asp acc gcg Thr Ala tgg tcc Trp Ser ggc acc Gly Thr 110 tcc act Ser Thr 125 ggc att Gly Ilie att atc Ile Ile Phe Gin ggg gga Gly Giy gtt ggc Val Giy 80 gta att Val Ile acc ttt Thr Phe act ggt Thr Gly ttc att Phe Ile 145 acc gag Thr Giu Asp gtg Val1 gac Asp gtc Val ggt Gly tcc Ser 130 cct Pro gcg Ala Asn c tg Leu atc Ile aac Asn tgt Cys 115 ggc Gly ggt Giy cca Pro PCT17B00100923 Ala Leu gtt atc 307 Val Ile att gca 355 Ile Aia gga gca 403 Gly Ala 100 aaa gcc 451 Lys Ala gaa cga 499 Giu Arg cat tac 547 His Tyr att aag 595 Ile Lys 165 taatttgttt tgacgacgca gta <210> 870 <211> 166 <212> PRT <213> Corynebacterium giutanicun <400> 870 Met Thr Gin Ser Ala Pro Giu Phe 1 5 Ile-Ilie Gly Asp Asn Ala Gin Ser Giy Gly Ala Thr Giu Phe His Gly 40 Gin Asp Asn Ala Leu Leu Lys Ser Gly Val Leu Vai Ile Asp Gly Asp 70 Gly Asp Ilie Ilie Ala Gly Leu Gly Ilie Val Asn Giy Ala Ile Arg Asp Asp Phe Val Asp Thr Trp Gly Val1 Asn Cys Pro Leu Gly Met Asp Leu Phe Gly Val Val Thr 1201 WO 01/00843 WO 0100843PCT/EBOO/00923 Phe Gly Cys 115 Gly Ser Gly 130 Lys Ala Leu Gly Asn Pro Arg Lys Ser 125 Thr Lys Thr Glu Arg Asp Val 135 Val Val Ser Ile Gly Ile Asp Phe Ilie Pro Gly His Tyr 145 Val1 150 Tyr Ala Asp Ser Gly Ile Ile Val Giu Ala Pro Ilie Lys Gin 165 <210> 871 <211> 621 <212> DNA <213> Corynebacterium <22 0> <221> CDS <222> (101)..(598) <223> FRXA02906 <400> 871 tttgtgggca atctggtttt giutamicum ttcgtaattg tgtgggatga atctcttaaa aattcacatt tagcaggaca agcatactgt tttagttcta tgctgtgggc atg act caa agt Met Thr Gin Ser cca gaa ttc att gcc ace gca gac ctc Pro Giu Phe Ile Ala Thr Ala Asp Leu gac atc atc ggc Asp Ile Ile Gly gac aac Asp Asn gcg caa tca Ala Gin Ser ttc eac gga Phe His Gly gac act cag ttt caa aac ctt gga ggt Asp Thr Gin Phe Gin Asn Leu Gly Giy gcc aca gaa Ala Thr Glu aac gcc ctc Asn Ala Leu ata ata acc acc Ile Ile Thr Thr aaa tgc ttc caa Lys Cys Phe Gin ctg aaa tee atc ctg agc Leu Lys Ser Ile Leu Ser gag Giu gat aat ect ggg Asp Asn Pro Gly gtg ctg gtt ate Val Leu Val Ile gat Asp ggc gac gca tcc Giy Asp Ala Ser cac ace gcg eta His Thr Ala Leu ggc gac atc att Gly Asp Ile Ile gga ett gga aaa Gly Leu Gly Lys cat ggt tgg tcc His Gly Trp Ser gta att gtc aae Val Ile Val Asn gga gca Gly Ala 100 att cga gac Ile Arg Asp gca gte ate gge ace atg ace ttt ggt Ala Val Ile Gly Thr Met Thr Phe Gly 110 tgt aaa gcc Cys Lys Ala 115 ett gga ace aac ccg egg aaa tee act aaa act ggt tee gge gaa ega 1202 WO 01/00843 PCTIBOO/00923 Leu Gly Thr Asn Pro Arg Lys Ser Thr Lys Thr Gly Ser Gly Giu Arg 120 125 130 gac gta gtg gta tcg att ggt ggc att gac ttc att cct ggt cat tac 547 Asp Val Val Val Ser Ilie Gly Gly Ilie Asp Phe Ile Pro Gly His Tyr 135 140 145 gtc tac gcg gac tct gac gga att atc gtc acc gag gcg cca att aag 595 Val Tyr Ala Asp Ser Asp Gly Ile Ile Val Thr Giu Ala Pro Ile Lys 150 155 160 165 cag taatttgttt tgacgacgca gta 621 Gin <210> 872 <211> 166 <212> PRT <213> Corynebacteriun giutamicum <400> 872 Met Thr Gin Ser Ala Pro Glu Phe Ile Ilie Gly Gin Gly Gly Ilie Phe G ly Ilie 145 Glu Asn Giu Leu Ile Ala Ala Ala Arg Tyr Lys 165 Gin His Lys Gly Leu Arg Gly Val 135 Tyr Ser Gly Ser Asp Gly Asp Thr 120 Val Ala Ala 10 Asp Ile Leu Ser Asp 90 Ala Pro Ser Ser Ala Gin Thr Glu His Gly Ilie Lys Gly 140 Gly Asp Phe Val Asp Thr Trp, Gly Ser 125 Gly Ile Leu Gin Lys Asn Ala Ser Thr 110 Thr Ile Ile Val Asn Cys Pro Leu Gly Met Lys Asp Val1 Asp Leu Phe Gly Val1 Va 1 Thr Thr Phe Thr 160 <210> 873 <211> 1752 <212> DNA <213> Corynebacteriun glutanicuin 1203 WO 01/00843 <220> <221> CDS <222> (101)..(1729) <223> RXA02315 <400> 873 cgtttggaaa cgcttgctgc cagcaaagat aggcgtgatt PCTIIBOO/00923 ggtggtttga gcgcgtgcgt gaatcgtatc cgtaectgga gacgatctag actgttgtge atg tec age acg eca Met Ser Ser Thr Pro gc t Ala act Thr gag Giu egc Arg ccg Pro ce t Pro tct Ser att Ile act Thr gc t Ala 150 gtt Val1 tgg Trp gag caa Gin gac Asp t tg Leu age Ser gtg Val1 gc t Ala get Ala aac Asn gag Giu 135 tee Ser gc t Ala acg Thr cac ctt Leu gtg Val1 geg Ala tca Ser gtg Val gct Ala cgt Arg 105 acc Thr gat Asp att Ile gaa Giu cgc Arg 185 etc gc Ala tta Leu egg Arg ttt Phe gtg Val1 gaa Giu cct Pro ggt Gly cag Gin ecg Pro ctg Leu 170 tgg Trp gtg gce Ala cca Pro gat Asp geg Ala 60 ace Thr gcg Ala cat His ttt Phe geg Ala 140 cat His gag Giu aae Asfl gee gtt Val1 gga Gly etg Leu etg Leu tee Ser cat His t tg Leu ggt Giy 125 eag Gin t te Phe ett Leu cac His ggt att Ile tc S er 30 cgt Arg tee Ser ggc Gly gc Ala gtg Val 110 ga t Asp att Ile aa t Asn cat His ggt Gly 190 gat gat Asp 15 agg Arg gte Val1 cta Leu aeg Thr cat His 95 gga Giy ctt Leu get Ala ett Leu ggt Gly 175 gag Giu gaa tee Ser aae Asn eat His geg Ala get Ala 80 ate Ile aeg Thr gea Ala gaa Giu gea Ala 160 gag Giu gtg Val gea 1 ete Leu tea Ser gtg Val egt Arg 65 gta Val1 eeg Pro ggg Gly ccg Pro age Ser 145 ett Leu gea Ala aee Thr tgg eca Pro ttg Leu 35 ate Ile eag Gin aae Asn att Ile age Ser 115 gte Val1 get Ala gtt Val gga Gly gae Asp 195 gtg eac His 20 teg Ser gae Asp gee Ala tge Cys gtg Val1 100 caa Gin ggt Giy eag Gin ect Pro gca Ala 180 etg Leu gaa gte Val ctt Leu gag Giu Arg etg Leu etc Leu aeg Thr ate Ile ggg Gly ttg Leu 165 tea Ser ggg Gly ggg 115 163 211 259 307 355 403 451 499 547 595 643 691 739 1204 WO 01/00843 Giu His Th ctg gaa ga Leu Glu As 215 aat ccg gt Asn Pro Va 230 tcc gcg ga Ser Ala Gi gtg gga ca Val Gly Hi cct ggc at Pro Giy Ii 28 ccc ggc cg 1027 Pro Giy Ax 295 acc cag ga 1075 Thr Gin GI 310 gcg gcc ga 1123 Ala Ala As acc ggc ct 1171 Thr Gly Le gat act ct 1219 Asp Thr Le 36 gtg ggt at 1267 Val Gly Me 375 gca ggc at 1315 Ala Gly Ii 390 Ala atc Ile 220 gct Ala gta Val cac His gtg Val cag Gin 300 cta Leu gac Asp gca Ala gca Ala ggc Gly 380 gtt Val1 Gly 205 gc t Ala gct Ala aac Asn cgc Arg ctt Leu 285 gtg Val aag Lys gtc Val gcc Ala tcc Ser 365 gtg Val gc t Ala Asp gaa Giu gaa Giu ac c Thr gga Gly 270 tca Ser ggc Gly atc Ile c tg Leu gtg Val 350 aac Asn gat Asp c aa Gin atc G iu CC t Pro atc Ile agg Arg 255 gtg Val cgc Arg agc Ser tgt Cys gac Asp 335 gcg Ala tca Ser acc Thr gca Ala cgc Ala act Thr ttg Leu 240 cct Pro t tg Leu acc Thr aca Thr tcg Ser 320 aac Asn gat Asp atc Ile ttc Phe atc Ilie 400 gcg Trp gca Ala 225 ctg Leu gat Asp aag Lys gat Asp gtg Val1 305 gca Ala caa Gin acc Thr cgt Arg tcc Ser 385 ggc Gly cca Pro Val1 aag Lys gag Giu gtg Val1 atg Met 275 atc Ile gtc Val1 tca Ser ttc Phe ggC Gly 355 ctc Leu cga Arg tca Ser act Thr PCT/EBO0100923 Giu Gly cct tat 787 Pro Tyr cag gtc 835 Gin Val 245 atc gtg 883 Ile Val 260 tca gat 931 Ser Asp act gat 979 Thr Asp acc ggc Thr Gly gaa ctt.

Giu Leu 325 ggt ttc Gly Phe 340 acc ggc Thr Gly tcc ctg Ser Leu ggt gtc Gly Val ctt gct.

Leu Aia 405 gtg gcc Val Ala gtg cag tcc cgc cac ccc gat gaa 1363 Val Gin Ser Arg His Pro Asp Giu Ile Arg Ala 1205 WO 01/00843 WO 0100843PCT/JIBOO/00923 ctt ctg 1411 Leu Leu ggc cct 1459 Gly Pro gac aac 1507 Asp Asn 455 ctc cgc 1555 Leu Arg 470 atc gcg 1603 Ile Ala aac ctc 165.

Asn Leu gga ttc 1699 Gly Phe caa cag 1749 Gin Gin 535 ggc gat Gly Asp 425 gat gaa Asp Giu ctg tcg ttc ctt cac Leu Ser Phe Leu His 430 cca cgc cca gaa aac Pro Arg Pro Glu Asn 445 gga atc ttc gaa ctc Giy Ile Phe Giu Leu 420 att ggc gga ctg ctc atc Ile Gly Gly Leu Leu Ile 435 acc atc gtg gtc tcc aac Thr Ile Val Val Ser Asn ctc Leu 440 ggt Gly ggc Giy cta gaa acc Leu Glu Thr 450 ggc gca gat ggt Gly Ala Asp Gly 460 ccc aac ttc gag Pro Asn Phe Giu 475 gat ctc tgc gca Asp Leu Cys Ala 490 cgt Arg ggc Gly 465 gct ttc ggt acc cca Ala Phe Gly Thr Pro 480 tac ggc att gaa cac Tyr Giy Ile Giu His cac gac gcg tcc His Asp Ala Ser 485 caa gtg gta gac Gin Vai Val Asp caa gac Gln Asp 505 ctc atc Leu Ile atc gcg Ile Ala 495 cta gtt gat acc acc gaa gta tcc Leu Vai Asp Thr Thr Giu Val Ser 510 515 acc gtc cga gat acc cgc cgt gca Thr Val Arg Asp Thr Arg Arg Ala 500 acc Thr 520 caa Gin att att gaa gct tcg Ile Ile Giu Ala Ser 525 gct ctc atg gac acg Aia Leu Met Asp Thr 540 gtg Vali gcg 1752 <210> 874 <211> 543 <212> PRT <213> Corynebacterium glutamicum <400> 874 Met Ser Ser Thr Pro Aia Gin Asp Leu 1 5 Leu Aia Pro His Val Thr Asp Vai Val 25 Ser Pro Leu Ser Leu Giu Leu Leu Ala 40 530 cac taaatggagt ggtaccaagt His Aia Arg Ala Val Ile Asp Ser 10 Leu Cys Pro Gly Ser Arg Asn Arg Gin Asp Leu Arg Vai His 1206 WO 01/00843 Val Arg Ile Arg Thr Gin Val Ala Asn Pro Leu Ile Gly Ala Ser 115 Pro Thr Val 130 Ser Leu Aia 145 Leu Asp Val Ala Val Gly Thr Vai Asp 195 Trp Giu Val 210 Ala Pro Lys 225 Leu Lys Giu Asp His Val Lays Leu Met 275 Asp Ile Ile 290 Vai Lys Vai 305 Ala Ala Ser Gin Giu Phe Thr Leu Gly 355 Arg Asp Leu Ser Val1 Ala Arg 105 Thr Asp Ile Glu Arg 185 Leu Val His Gly Pro 265 Lys His Lys Gly His 345 Phe Pro Phe Val1 Gi u Pro Gly Gin Pro Leu 170 Trp Val Pro Pro Tyr 250 Thr Leu Ala Gin Val1 330 Val1 Ala Phe Leu Met 75 Ala Ala Ile Val1 Arg 155 Pro Ile Ile Thr Leu 235 Val Leu Thr Asp Trp 315 Arg Ala Ala Asp Ser Gly Ala Val 110 Asp Ile Asn His Gly 190 Asp Giu Giu Thr Gly 270 Ser Gly Ile Leu Val 350 Asn Asp PCTJIBOO/00923 Leu Ala Thr Ala His Ile Gly Thr Leu Ala Ala Giu Leu Ala 160 Gly Glu 175 Giu Val Glu Ala Pro Thr Ile Leu 240 Arg Pro 255 Val Leu Arg Thr Ser Thr Cys Ser 320 Asp Asn 335 Ala Asp Ser Ile Thr Phe WO 01/00843 370 375 Ser Pro Arg Giy Val Ala Gly Ile Asp Giy Ser 385 390 395 Giy Thr Ser Leu Ala Val Gin Ser Arg His Pro 405 410 Pro Arg Thr Val Ala Leu Leu Gly Asp Leu Ser 420 425 Gly Giy Leu Leu Ile Gly Pro Asp Glu Pro Arg 435 440 Ile Val Val Ser Asn Asp Asn Gly Gly Gly Ile 450 455 Thr Gly Ala Asp Gly Leu Arg Pro Asn Phe Giu 465 470 475 Pro His Asp Ala Ser Ile Ala Asp Leu Cys Ala 485 490 His Gin Val Vai Asp Asn Leu Gin Asp Leu Ile 500 505 Thr Thr Giu Vai Ser Giy Phe Thr Ile Ile Giu 515 520 Asp Thr Arg Arg Ala Gin Gin Gin Ala Leu Met 530 535 <210> 875 <211> 1080 <212> DNA <213> Corynebacterium giutanicun <22 0> <221> CDS <222> (101)..(1057) <223> RXA02319 <400> 875 atgtgggtga gataaccgac cgtgatgtcg ccctagcaaa agaccttggc catttcggca gaggcttaag gttaaagatt PCT/iBOO/00923 380 Vai Asp Phe Pro Phe 460 Arg Gly Ile Aia Asp 540 Ala Arg 415 Asp Leu Leu Giy Ile 495 Val Val1 His Ile 400 Ala Ile Thr Giu Thr 480 Giu Asp Arg acc gac aac Thr Asp Asn gaa gaa ttc Giu Glu Phe gcc gat ggc Aia Asp Gly cct ttt gat ccc acc Pro Phe Asp Pro Thr acc gac atc acc tac Thr Asp Ile Thr Tyr atc gtg cgc atc gcc Ile Vai Arg Ile Ala 45 caa Gin cac His 30 t tc Phe tgg gcc Trp Aia 15 cgc cac Arg His gac cgc Asp Arg agtcatcgac gcccacgcca atg agc aac tac agc Met Ser Asn Tyr Ser 1 acc gtt cca ggt ttt Thr Val Pro Gly Phe gtg ggc acc acc cgc Vai Giy Thr Thr Arg ccc gaa gtt cgc aat Pro Giu Val Arg Asn 1208 WO 01/00843 WO 0100843PCT/iBOO/00923 gct ttc Ala Phe cgc ccc cac acc Arg Pro His Thr gac gag ctt tac Asp Glu Leu Tyr gcc ctc gac cac Ala Leu Asp His cgc cgg acc cca Arg Arg Thr Pro gtt gga acc atc Val Gly Thr Ile ctg Leu ctc acc ggc aac Leu Thr Gly Asn ccc agc gaa aaa Pro Ser Glu Lys ggt ggc tgg gcg Gly Gly Trp Ala tgc tcc ggc ggc Cys Ser Gly Gly gac caa Asp Gin 100 cgc atc cgc Arg Ile Arg gac gat gcc Asp Asp Ala 120 ggg Gly 105 cgc tcc ggc tac Arg Ser Gly Tyr tac gcc acc gaa Tyr Ala Thr Giu cac gcg cgc His Ala Arg 115 cgc acc aaa Arg Thr Lys acc gct gat gtc Thr Ala Asp Val ttc Phe 125 acg gta gat att Thr Val Asp Ile gcc Ala 130 gtt gaa Val Giu 135 ggc gga cgc ctc Giy Gly Arg Leu att ttg gaa gtc Ile Leu Glu Val, caa Gin 145 cgc ctc atc cgc Arg Leu Ile Arg atg cct aaa gtt gtc atc gca gta gtc Met Pro Lys Vai Val Ile Ala Val Val 155 aac Asn 160 ggc tgg gca gcc Giy Trp Ala Ala ggc Giy 165 ggt ggg cac tcc Gly Gly His Ser c tc Leu 170 cat gtc gtt tgc His Val Val Cys ctc acc atc gct Leu Thr Ile Ala tcc cgc Ser Arg 180 caa gaa gca Gin Glu Ala gct ggc tac Ala Gly Tyr 200 cgc Arg 185 ttc aag caa acc Phe Lys Gin Thr gct gac gtg gga Ala Asp Val Gly tcc ttc gac Ser Phe Asp 195 cag aaa aac Gin Lys Asn ggc tcc gcc tac Gly Ser Ala Tyr gcg aaa atg gtc Ala Lys Met Val gga Gly 210 gcc cgc Ala Arg 215 gaa atc ttc ttc Giu Ile Phe Phe gga cgc acc tac Gly Arg Thr Tyr gcc gaa cgc atg Ala Giu Arg Met caa Gin 230 caa atg ggc gca Gin Met Gly Ala gtc Val 235 aac atc gtg gcc Asn Ile Val Ala cac ggc gac cta His Gly Asp Leu aaa gaa gcc atc Lys Giu Ala Ile caa Gin 250 gca gcc cgc gaa Ala Ala Arg Giu aac acc aaa tcc Asn Thr Lys Ser ccc acc Pro Thr 260 ggg caa cgc Gly Gin Arg atg gga caa Met Gly Gin 280 atg Met 265 ctg aaa ttc gcc Leu Lys Phe Ala aat ctc acc gac Asn Leu Thr Asp gat ggc ctc Asp Gly Leu 275 gcc tac atg Ala Tyr Met caa gtc ttc gcc Gin Val Phe Ala gaa gcc acc cgc Giu Ala Thr Arg 1209 WO 01/00843 PCTIBOO/00923 acg gat gaa gcc gta gag ggt aag gaa gca ttc cta gaa aag cgc gaa 1027 Thr Asp Giu Ala Val Giu Gly Lys Giu Ala Phe Leu Glu Lys Arg Glu 295 300 305 ccc aac tgg aat gaa ttc cct tac tac tac tagtgagttc atggggtcct 1077 Pro Asn Trp Asn Giu Phe Pro Tyr Tyr Tyr 310 315 aaa 1080 <210> 876 <211> 319 <212> PRT <213> Corynebacteriun giutainicum <400> 876 Met Ser Asn Tyr Ser Thr Asp Asn Pro 1 Thr Val1 Pro Gin Leu Ser Thr Ile Gin 145 Gly Thr Val1 Val G iy Glu Ala Thr Giy Giu Ala 130 Arg Trp Ile Gly Pro Thr Val1 Leu Gly Giy His 115 Arg' Leu Ala Ala Ser Phe Gly Arg Arg Giu Arg Aia 120 Gly Pro His Al a Tyr 200 Thr Ile Pro Thr Lys Gly 105 Thr Gly Lys Ser Arg 185 Gly Ile Phe 10 Asp Vali His Pro Asp Arg Ala Arg Val1 Leu 170 Phe Ser Phe Trp Arg Asp Leu Ile Phe Tyr Val Giu Val Asp 175 Ala Lys Thr Ala His Arg Tyr Leu Cys Ala Asp Val Asn 160 Leu Asp Met Tyr 195 Val Gly 210 Gin Lys Asn Ala Arg Glu 215 1210 WO 01/00843 WO 0100843PCT/iBOOIOO923 Asp Ala Giu Arg Met Gin Gin Met Gly Ala Val 225 230 235 His Gly Asp Leu Glu Lys Giu Ala Ile Gin Ala 245 250 Thr Lys Ser Pro Thr Gly Gin Arg met Leu Lys 260 265 Thr Asp Asp Gly Leu Met Gly Gin Gin Vai Phe 275 280 Arg Leu Ala Tyr Met Thr Asp Glu Ala Val Giu 290 295 Leu Giu Lys Arg Glu Pro Asn Trp Asn Giu Phe 305 310 315 <210> 877 <211> 1017 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(994) <223> RXS00393 <400> 877 tctattcatt tcacaatagc gtttcacact cccccatagc aattgcgcga tcgagtatgt gatggggaaa gatagaggtt Asn Ala Phe Ala G ly 300 Pro Val Giu Phe 270 Glu Glu Tyr ccc Pro ccg Pro gtC Val Ct t Leu tac Tyr cga Arg aat Asn tgg Trp ttt Phe gcg Ala ggc Gly ggt Gly tc t Ser gca Ala cat His tgg Trp att Ile tct Ser gta act ttg tcc Val Thr Leu Ser ctgccgaacg tatttcaagc atg tct cac acg gaa Met Ser His Thr Glu 1 att caa ggc gca cgc Ile Gin Gly Ala Arg att gcc ggt tca ggt Ile Ala Gly Ser Gly aag gcc ttg ctg gcg Lys Ala Leu Leu Ala aat tac gcc aat gat Asn Tyr Ala Asn Asp cgc Arg aaa Lys acc Thr gtg Val cct Pro gct Ala 100 WO 01/00843 gcg ttt att Ala Phe Ile ctg ttg agc Leu Leu Ser 120 PCTIBOOIOO923 ttc ggt atc gca Phe Gly Ile Ala gtc gcc ggc acc Val Ala Gly Thr gcg ctg agc Ala Leu Ser 115 tgt gtg ctg Cys Val Leu gcg tgg tgg ctg Ala Trp Trp, Leu ctc atc ggc atc Leu Ile Gly Ile ctg Leu 130 ggc gcg Gly Ala 135 tgg ttc tac acc Trp Phe Tyr Thr ggt aaa aat cct Gly Lys Asn Pro ggt tac cgc ggg Gly Tyr Arg Gly ggc gag att gct Gly Glu Ile Ala gtg Val1 155 ttc atc ttc ttc Phe Ile Phe Phe ctc gtc gcg gtc Leu Val Ala Val gga acg cag ttc Gly Thr Gin Phe caa acc ggt tcc Gin Thr Giy Ser agc tgg gcc ggt Ser Trp Ala.Gly ttg gcc Leu Ala 180 gcc gca gtt Ala Ala Val aat att cgc Asn Ile Arg 200 gtg ggg tcg atg Val Gly Ser Met gct ggc gtg aac Ala Giy Vai Asn ttg gcc aac Leu Ala Asn 195 att acc ctc Ile Thr Leu gat att cca acc Asp Ile Pro Thr gat Asp 205 agc aag acc gga Ser Lys Thr Gly aaa Lys 210 gcg gtc Ala Val 215 cgc ctg ggc gat Arg Leu Giy Asp ggt gct cgt aag Gly Ala Arg Lys ttc ctc gcg ctg Phe Leu Ala Leu tcc acg ccg ttc Ser Thr Pro Phe atc Ile 235 atg tcc atc tgc Met Ser Ile Cys gcg ttt gtc gcc Ala Phe Val Ala tgg Trp 245 cca gcg ctg atc Pro Ala Leu Ile gcg Ala 250 atc atc gtt ttc Ile Ilie Val Phe ctg gca ctg aaa Leu Ala Leu Lys gcc gca Ala Ala 260 ggg ccg atc Gly Pro Ile ggc tca aca Gly Ser Thr 280 aac aac gcc acc Asn Asn Ala Thr ggc Gly 270 aag gat ctc atc Lys Asp Leu Ile ccc gtc atc Pro Val Ile 275 acg ggc ctg Thr Gly Leu ggg cgc gcc atg Gly Arg Ala Met gcg Al a 285 ttg tgg gcc gtg Leu Trp, Ala Val ctc Leu 290 gca tta gcg ttt agc taaaacgctt ttcgacgctc ccc 1017 Ala Leu 295 Ala Phe Ser <210> 878 <211> 298 <212> PRT <213> Corynebacteriun giutamicun <400> 878 Met Ser His Thr Giu Pro Gin Pro Asn Ser Val Thr Leu Ser Asp Trp 1212 WO 01/00843 1 Ile Ile Lys Asn Arg Lys Gly Ile Tyr 145 Leu Trp Val Gly Leu 225 Ala Ala Leu Val Gly Gly Leu Ala Gly Lys Ala 115 Cys Tyr Ala Gly Leu 195 Ilie Leu Val Lys Pro 275 Thr Arg Gly Ala Asp Leu Ala Ser Leu Gly Met 165 Ala Asn Leu Leu Trp 245 Ala Ile Leu Pro Val1 Leu Tyr 70 Arg Ala Leu Gly Leu 150 Gly Ala Asn Ala Ile 230 Pro Gly Gly Ala Arg Ala Val Ser Leu Phe Leu Ala 135 Gly Thr Ala Ile Val 215 Ser Ala Pro Ser Leu 295 10 Ala His Trp Ile Ser 90 Phe Trp, Tyr Ala Thr 170 Val1 Ile Gly Phe Ala 250 Asn Arg Ser Ala Gly Leu Gly Leu Ile Leu Gly 140 Phe Thr Ser Thr Ala 220 Met Ile Ala Met Phe Phe Ile Thr Ala Ala Ile 125 Gly Ile Gly Met Asp 205 Gly Ser Val1 Thr Ala 285 Ala Val Ile Asp Glu Gly 110 Leu Lys Phe Ser Ser 190 Ser Ala Ile Phe Gly 270 Leu PCTIBOO/00923 Pro Val Trp Trp, Gly Val Glu Asp Pro Lys Val Ala Ile Gly Asn Pro Phe Gly 160 Val Ser 175 Ala Gly Lys Thr Arg Lys Cys Leu 240 Pro Leu 255 Lys Asp Trp Ala <210> 879 <211> 1005 <212> DNA <213> Corynebacterium giutamicum 1213 WO 01/00843 WO 0100843PCTfIBOO/00923 <220> <221> CDS <222> (101)..(982) <223> FRXA00393 <400> 879 tctattcatt tcacaatagc gtttcacact cccccatagc Ctgccgaacg tatttcaagc aattgcgcga tcgagtatgt gatggggaaa gatagaggtt atg tct cac acg Met Ser His Thr 115 163 ccc cag ccg aat tct gta act ttg tcc gat tgg att caa ggc Pro Gin Pro Asn Ser Val Thr Leu Ser Asp Trp Ile Gin Gly gca cgc Ala Arg ccg cgt acc Pro Arg Thr gtc gcc gct Val Ala Ala gca aat gcg ttc Ala Asn Ala Phe cct gtc att gcc Pro Val Ile Ala ggt tca ggt Gly Ser Gly ttg ctg gcg Leu Leu Ala ttt cat gat ggt Phe His Asp Gly ttt Phe gtg tgg tgg aag Val Trp Trp Lys ctt gtc* Leu Val gtg gcg tgg gct Val Ala Trp Ala atc atc ggt gtg Ile Ile Gly Val tac gcc aat gat Tyr Ala Asn Asp tac Tyr tct gat ggc att Ser Asp Gly Ile ggc acc gat gaa Gly Thr Asp Glu cgc acc ggt cct Arg Thr Gly Pro 307 355 403 cga ctc act ggt Arg Leu Thr Gly ggg ttg gct gag Gly Leu Ala Giu aag aaa gtg aaa Lys Lys Val Lys gct gcg Ala Ala 100 gcg ttt att Ala Phe Ile ctg ttg agc Leu Leu Ser 120 tct Ser 105 ttc ggt atc gca Phe Gly Ile Ala ggt Gly 110 gtc gcc ggc acc Val Ala Gly Thr gcg ctg agc Ala Leu Ser 115 gcg tgg tgg ctg Ala Trp Trp Leu atc Ile 125 ctc atc ggc atc ctg tgt gtg ctg Leu Ile Gly Ile Leu Cys Val Leu 130 ggc gcg Gly Ala 135 tgg ttc tac acc Trp Phe Tyr Thr ggc Gly 140.

ggt aaa aat cct tat ggt tac cgc ggg Gly Lys Asn Pro Tyr Gly Tyr Arg Gly 145 ctc ggc gag att gct Leu Gly Giu Ile Ala ttc atc ttc ttc Phe Ilie Phe Phe ctc gtc gcg gtc Leu Val Ala Val gga acg cag ttc Gly Thr Gin Phe caa acc ggt tcc Gin Thr Gly Ser gtc Val 175 agc egg gcc ggt Ser Trp Ala Gly ttg gcc Leu Ala 180 gcc gca gtt Ala Ala Val ggc Gly 185 gtg ggg tcg atg Val Gly Ser Met tct Ser 190 gct ggc gtg aac Ala Gly Val Asn ttg gcc aac Leu Ala Asn 195 1214 woo0 aat Asn gcg Ala att Ile 230 cca Pro ggg Gly gc t Ala cat 1005 His 1/00843 att cgc gat att cca acc g Ile Arg Asp Ile Pro Thr A 200 2 gtc cgc ctg ggc gat gcg g Vai Arg Leu Gly Asp Ala C 215 *220 tcc acg ccg ttc atc atg t.

Ser Thr Pro Phe Ile Met S 235 gcg ctg atc gcg atc atc g Ala Leu Ile Ala Ilie Ile V.

250 ccg atc cgc aac aac gcc a Pro Ile Arg Asn Asn Ala T 265 caa cag ggc gcg cca tgg c Gin Gin Gly Ala Pro Trp A 280 2 tagcgtttag ctaaaacgct ttt PCTIIBOO/00923 att acc ctc 739 Ile Thr Leu at sp 05 gt iy cc er acc gga Thr Giy aag ctg Lys Leu 225 ctg gcg Leu Aia 240 ctg gca Leu Aia gat ctc Asp Leu ccg tgc Pro Cys aaa Lys 210 ttc Phe ttt Phe ctg Leu atc Ile tca Ser 290 <210> 880 <211> 294 <212> PRT <213> Corynebacterium giutamicun <400> 880 Met Ser His Thr Giu Pro Gin Pro 1 Ile Gin Gly Ala Arg Pro Arg Thr Ilie Ala Gly Ser Giy Vai Ala Ala Lys Ala Leu Leu Ala Leu Val Vai 55 Asn Tyr Aia Asn Asp Tyr Ser Asp 70 Arg Thr Gly Pro Leu Arg Leu Thr Lys Val Lys Ala Ala Ala Phe Ile 100 Gly Thr Aia Leu Ser Leu Leu Ser 115 120 Ile Leu Cys Val Leu Gly Ala Trp Val Asn Asp Ala Arg C ly Gly Trp Thr Thr Ala Gly Leu Giy Leu Ile Leu Giy 1215 WO 01/00843 130 135 Tyr Gly Tyr Arg Gly Leu Gly Giu Ile Ala Val 145 150 155 Leu Val Ala Vai Met Gly Thr Gin Phe Thr Gin 165 170 Ti-p Ala Gly Leu Ala Ala Ala Val Gly Val Gly 180 185 Val Asn Leu Ala Asn Asn Ilie Arg Asp Ile Pro 195 200 Gly Lys Ilie Thr Leu Ala Val Arg Leu Gly Asp 210 215 Leu Phe Leu Ala Leu Ile Ser Thi- Pro Phe Ile 225 230 235 Ala Phe Val Ala Trp Pro Ala Leu lie Ala Ile 245 250 Ala Leu Lys Ala Ala Gly Pro Ile Arg Asn Asn 260 265 Leu Ilie Pro Ser Ser Ala Gin Gin Gly Ala Pro 275 280 Cys Ser Arg Ala Ti-p His 290 <210> 881 <211> 843 <212> DNA <213> Corynebacterium glutatnicum <2 <221> CDS <222> (101) (820) <223> RXA00391 <400> 881 atcttgtaga tcccaccgca attctgggag atctcgagga tttcctcccc atccctgtac aagataaaac ccgtgcacag caa cga gtt ggc ctc gcc atc gat cct tcg atc Gin Arg Val Gly Leu Ala Ile Asp Pro Ser Ile 15 act tct ggt tct aca ggt acc ccg aag ggc gct Thr Ser Gly Ser Thr Gly Th- Pro Lys Gly Ala 30 aat ttg gtg agt tcc gcc gat gct. acg cat cag Asn Leu Val Ser Ser Ala Asp Ala Thi- His Gin 45 PCTIIBOO/00923 140 Phe Th- Ser Thr Ala 220 Met Ile Aia Ti-p Ile Gly Met Asp 205 Gly Ser Val1 Thr Arg 285 Phe Ser Ser 190 Ser Ala Ile Phe Gly 270 Cys cgcaatctct ggaagaaaac ttg ctg cgc gat tct Leu Leu Arg Asp Ser 1 gct ttg gtg atg gcc Ala Leu Val Met Ala cag ctc act ccg ttg Gin Leu Thr Pro Leu ttt tta ggt ggc gaa Phe Leu Gly Gly Glu 1216 WO 01/00843 WO 0100843PCTIiBOO/00923 ggc cag Gly Gin tgg ttg ctt gcc Trp Leu Leu Ala atg Met 60 cca gca cac cac Pro Ala His His att Ile gca ggc atg cag Ala Gly Met Gin gtg Vai ctt ctt cga agc Leu Leu Arg Ser att gct gga gtt Ile Ala Gly Val gag Giu cca cta gct att Pro Leu Ala Ile cic agc aca ggt Leu Ser Thr Giy cac att gac gct His Ile Asp Ala gca ggc gcc gcg Ala Gly Aia Ala gca gaa Ala Giu 100 ctg aaa aat Leu Lys Asn tta ctt aaa Leu Leu Lys 120 acc Thr 105 ggrc gac cgc gtc tat aca tcc ttg act Giy Asp Arg Val Tyr Thr Ser Leu Thr 110 cca atg cag Pro Met Gin 115 ctg aaa ctt Leu Lys Leu gca atg gac tcc Ala Met Asp Ser t tg Leu 125 caa ggc att gaa Gin Giy Ile Giu gcc Ala 130 ttt gat gtc att ctt gtt Phe Asp Val Ile Leu Val ggc Gly 140 ggt gct gca ttg Gly Ala Ala Leu tct Ser 145 aag cag gcc cga Lys Gin Ala Arg att tct gcg gag cag Ile Ser Ala Giu Gin 150 cta Leu 155 gac atc aac att Asp Ilie Asn Ile acc acc tac ggc Thr Thr Tyr Gly tcc Ser 165 tca gag act tca Ser Giu Thr Ser ggt Giy 170 ggc tgc gtt tat Giy Cys Val Tyr ggc aag ccc att Gly Lys Pro Ile ccc ggc Pro Giy 180 gcg aaa gtc Ala Lys Val att gcg cag Ile Ala Gin 200 cgt Arg 185 att tcg gat gag Ile Ser Asp Giu cgc Arg 190 att gag ttg ggt Ilie Giu Leu Gly ggc ccg atg Gly Pro Met 195 ttc gcc aac Phe Ala Asn ggc tac aga aat Gly Tyr Arg Asn gca Ala 205 cct gaa cat ccg Pro Giu His Pro gag ggt Glu Gly 215 tgg ttt acc acc Trp Phe Thr Thr tct gat tca ggt gaa ctc cac gac ggg att Ser Asp Ser Gly Giu Leu His Asp Gly Ile 220 225 ctc Leu 230 tgc acc gtg act ggt Thr Val Thr Giy cgc Arg 235 gtg gat acc cgt Val Asp Thr Arg tgattccggt ggattgaagt 840 <210> 882 <211> 240 <212> PRT <213> Corynebacteriun giutamicum <400> 882 Leu Leu Arg Asp Ser Gin Arg Val Gly 1 5 Leu Ala Ile Asp Pro Ser Ile 10 1217 WO 01/00843 Ala Leu Val Gin Leu Thr Phe Leu Gly Ile Ala Gly Pro Leu Ala Gly Ala Ala Leu Thr Pro 115 Giu Ala Leu 130 Ser Lys Gin 145 Thr Thr Tyr Lys Pro Ile Leu Gly Gly 195 Pro Asp Phe 210 Leu His Asp 225 Met Pro Gly Met Ile Ala 100 Met Lys Ala Gly Pro 180 Pro Ala Gly Ala Leu Giu Gin Asp Giu Gin Leu Arg Ser 165 Gly Met Asn Ile Thr Asn Gly Val 70 Leu Leu Leu Phe Ile 150 Ser Ala Ile Giu Leu 230 Ser Leu Gin 55 Leu Ser Lys Leu Asp 135 Ser Giu Lys Ala Gly 215 Thr Ser 25 Ser Leu Arg Gly Thr 105 Ala Ile Giu Ser Arg 185 Gly Phe Thr Thr Ser L eu Ser Phe 90 Gly Met Leu Gin Gly 170 Ile Tyr Thr Gly Gly Ala Ala Leu 75 His Asp Asp Val Leu 155 Gly Ser Arg Thr Arg 235 Thr Asp Met Ile Ile Arg Ser Gly 140 Asp Cys Asp Asn Ser 220 Val Lys Thr Ala Gly Ala Tyr 110 Gin Ala Asn Tyr Arg 190 Pro Ser Thr PCTIBOO/00923 Gly Ala His Gin His His Val Giu Phe Ala Thr Ser Gly Ile Ala Leu Ile Val 160 Asp Gly 175 Ile Giu Giu His Gly Giu Arg His 240 <210> 883 <211> 384 <212> DNA <213> Corynebacterium giutamicui <22 0> <221> CDS <222> (101)..(361) <223> RXS02908 <400> 883 gccaacgagg gttggtttac cacctctgat tcaggtgaac tccacgacgg gattctcacc gtgactggtc gcgtggatac ccgtcattga ttccggtgga ttg aag ttg cac cca 115 Leu Lys Leu His Pro 1218 WO 01/00843 WO 0100843PCT/iBOO/00923 gag Glu tgt Cys gcg Ala gat Asp t tg Leu ctg Leu gta Val1 gtt Val1 tac Tyr cta Leu ccc Pro ttt Phe ctg gaa cgt gcc atc gca gi Leu Glu Arg Ala Ile Ala A! gtg ggt att ccc gat ccc q~ Val Gly Ilie Pro Asp Pro Ak tcc gga tcg atc agt ccg t( Ser Gly Ser Ile Ser Pro S( 45 cct cgt tgg cag ctt ccc a4 Pro Arg Trp Gin Leu Pro L agc att ggt cct gga aaa g( Ser Ilie Gly Pro Giy Lys A: tagtcttcat tcttgctggc tgc gtc Vali gca Ala gaa Glu cat His gc t Ala acc Thr att Ile ggc Giy ctg Leu atc Ile <210> 884 <211> 87 <212> PRT <213> Corynebacteriun giutamicum <400> 884 Leu Lys Leu His Pro Giu Val Leu Giu Arg Ala 1 5 10 Gly Vai Thr Ala Ala Cys Val Val Gly Ile Pro 25 Gin Ala Ilie Val Aia Ala Tyr Ser Giy Ser Ile 40 Ilie Giu Giy Leu Asp Asp Leu Pro Arg Trp Gin 55 Lys His Leu Giu Ser Leu Pro Ser Ilie Gly Pro 70 75 Arg Ala Ile Ala Lys Leu Phe Ile Aia Asp Ile Lys Asp Pro Arg Leu Giy Ser Pro Ser Giu Val Leu Pro Lys Arg Leu Giy Lys Ala Asp Arg <210> 885 <211> 705 <212> DNA <213> Corynebacterium giutamicum <22 0> <221> CDS <222> (101)..(682) <223> RXA00997 1219 WO 01/00843 WO 0100843PCTJIBOO/00923 <400> 885 cccatctgtg gcagatcgaa tgcacccacc gcggagaagt cgtcgcacga accacactgc gcaccatggt gctgaacaag tagccctata ctcgggcacc atg act aca tgg aaa Met Thr Thr Trp Lys gag Glu tgg Trp ctc Leu tgc Cys gtt Val1 gac Asp atc Ile ggC Gly cgc Arg cgt Arg 150 ctc Leu caa Gin aca Thr aa c Asn gat Asp cag Gin Gly cca Pro gag G iu ctc Leu 120 ctg Leu tgg Trp t tg Leu tct S er gat Asp C tc Leu gcc Ala gga Gly aca Thr gaa Glu act Thr 105 gc t Aia ggc Gly gtc Val gaa Giu gag Giu cca Pro gca Ala ccc Pro ggt Gly gat Asp tgg Trp gat Asp ggt Giy ggc Gly 140 gac Asp ttt Phe gcg Ala ggc Gly agg Arg 45 ggc Gly ccc Pro gtg Val c tc Leu cgc Arg 125 cgc Arg ttc Phe gag Glu tca Ser gat Asp gcg Ala c tg Leu ctg Leu gga Gly gtc Val1 cct Pro gtc Val gaa Giu tgg Trp 175 gaa Glu att Ile aaa Lys tcc Ser att Ile gat Asp t cc Ser gca Ala atc Ile gtc Vai 160 gat Asp 1 aac Asn tac Tyr atc Ile aag Lys gat Asp ctc Leu gcc Ala cta Leu ggt Gly 145 gca Ala ctt Leu gcg Ala gaa Giu gat Asp ggc Gly gcc Ala gtt Val aac Asn 115 aac Asn ggc Gly cgc Arg cc t Pro 115 163 211 259 307 355 403 451 499 547 595 643 692 ttc tta gtg gcg gta ttt acc aag aag Phe Leu Val Ala Val Phe Thr Lys Lys 190 taacacctct atcttgcacc tga <210> 886 <211> 194 1220 WO 01/00843 PCT/iBOO/00923 <212> PRT <213> Corynebacterium glutanicum <400> 886 Met Thr Thr Trp Lys Glu Leu Thr Asp Asn Asn Pro Ala His Ser Glu 1 Asn Ile Lys Asp Leu Ala Leu Gly 145 Ala Ala Glu Asp Gly Ala Val1 Asn 115 Asn Gly Arg Gin Ala Ala His Lys Asp i00 Val Ile Ala Val Asn Asp 40 Gln Gly Pro Glu Leu 120 Leu Trp Leu Leu 25 Ala Gly Thr Glu Thr 105 Ala Gly Val Glu Ala Ala Ile Leu Arg Phe Asp Asp Gly.

155 Ala Ala Pro Gly Asp Trp Asp Gly Gly 140 Asp Phe Gly Asn Arg Gly Gly Tyr Pro Ile Val Val Leu Ile 110 Arg Glu 125 Arg Ala Phe Leu Glu- Ser Lys Pro Phe 180 Lys Val Gin Gly Ser Phe Leu Val Ala Val Phe Thr 190 <210> 8817 <211> 861 <212> DNA <213> Corynebacteriun glutamicum <22 0> <221> CDS <222> (101)..(838) <223> RXA02189 <400> 887 aatggcaaaa atgggcacat acgatactga tgggaccaat gggaaaacct caagccacca attgagccag ttctctcaag caaaccgata ctggtgggat gtg gac gcg gcc gac 115 Val Asp Ala Ala Asp 1 1221 WO 01/00843 WO 0100843PCTIBOO/00923 tat cac gaa cgc Tyr His Giu Arg cct tct tat tta Pro Ser Tyr Leu acg gat tcc gct Thr Asp Ser Ala cac ggc His Gly gag ttc tac Glu Phe Tyr ctt ggc act Leu Gly Thr tgg Trp, tgc ccc gag atg Cys Pro Giu Met cat gaa aaa gat His Giu Lys Asp gta cgt ctt Val Arg Leu gag atc ggt Giu Ile Gly ccc gca gca ctc Pro Ala Ala Leu ggc aag aaa att Giy Lys Lys Ile tta Leu tgc ggc Cys Gly tcg gca ccg tgt Ser Ala Pro Cys gct Ala cgg tgg ctg gcc Arg Trp Leu Ala gat gtt ccg aat Asp Val Pro Asn ttt gtc acc gct Phe Val Thr Ala gac att tct tca Asp Ile Ser Ser caa Gin atg ctc aaa tac Met Leu Lys Tyr ggt cac gac cat Gly His Asp His gta cac ctc gta Val His Leu Val gcc gat gca atg Ala Asp Ala Met tca ctc Ser Leu 100 ccc tac gcc Pro Tyr Ala atc ccc ttt Ile Pro Phe 120 gac Asp 105 agt tcc ttt gac Ser Ser Phe Asp gtc ttt tcc gtt Val Phe Ser Val ttc ggc gcc Phe Gly Ala 115 atc gcg cgc Ile Ala Arg gtg gag gat tcc Val Giu Asp Ser gca ctc atg aag Ala Leu Met Lys gaa Glu 130 gtc ctc Val Leu 135 aaa ccc ggc gga Lys Pro Gly Gly ctc att ttc tcc Leu Ile Phe Ser acc cac ccg atg Thr His Pro Met tgg att ttc ctc Trp Ilie Phe Leu gac Asp 155 gat ccc ggc ccc Asp Pro Gly Pro ggc ctc acc gcg Gly Leu Thr Ala acc agc tac ttc Thr Ser Tyr Phe gac Asp 170 cag cgc ggc tac Gin Arg Gly Tyr gaa gaa gac gag Giu Giu Asp Glu gaa acc Giu Thr 180 ggt gct tta Gly Ala Leu aat gag ctt Asn Glu Leu 200 agc tat gcg gaa cag cac cgc acc atg ggc gcg cgg atc Ser Tyr Ala Giu Gin His Arg Thr Met Gly Ala Arg Ile 185 190 195 atc gac gcc tcc Ile Asp Ala Ser cac tta gat cac His Leu Asp His atc gaa cca Ile Giu Pro gaa tgg Glu Trp 215 cca gat gag ttg Pro Asp Glu Leu gaa aac tgg ggc Glu Asn Trp Gly tgg tca cca ctt Trp, Ser Pro Leu gga aag ctc ttc Gly Lys Leu Phe ccc Pro 235 ggg aca gca atc ttc ctc gcc acg tac Gly Thr Ala Ile Phe Leu Ala Thr Tyr ccc taaaaaacca acggcgctca ttt 1222 WO 01/00843 Pro PCTA0300/00923 <210> 888 <211> 246 <212> PRT <213> Corynebacterium glutamicun <400> 888 Val Asp Ala Ala Asp Tyr His Glu Arg 1 Asp Lys Ile Asn Met Asp Ser Lys Ile 145 Gly Giu Met His Gin 225 Leu Ser Asp Leu Asp Leu Ala Val Giu 130 Thr Leu Asp Gly Leu 210 Trp Ala Ala Val Giu Val Lys Met Phe 115 Ile His Thr Giu Ala 195 Ile Ser Thr 5 Gly Leu Gly Asn Ala Leu Ala Arg Met Ile 165 Thr Ile Pro Leu Arg 245 Glu Leu Cys Ala 70 Gly Pro Ile Val1 Arg 150 Thr Gly Asn Glu Arg 230 Pro Phe Gly Gly 55 Phe His Tyr Pro Leu 135 Trp Ser Ala Glu Trp 215 Gly Tyr Trp Thr Pro 40 Ser Ala Val Thr Asp His Ala Asp 105 Phe Val 120 Lys Pro Ile Phe Tyr Phe Leu Ser 185 Leu Ile 200 Pro Asp Lys Leu His 10 Cys Ala Pro Ala Asn 90 Ser Glu Gly Leu Asp 170 Tyr Asp Glu Phe Pro Pro Al a Cys Phe 75 Val Ser Asp Gly Asp 155 Gin Ala Ala Leu Pro 235 Ser Glu Leu Ala Asp His Phe Ser Arg 140 Asp Arg Glu Ser Giu 220 Gly Tyr Leu Met Leu Ser Gly Arg Trp, Ile Ser Leu Val Asp Val 110 Ala Ala 125 Leu Ile Pro Gly Gly Tyr Gin His 190 Leu His 205 Glu Asn Thr. Ala Gly His Lys Leu Ser Gin Val Leu Phe Pro Val 175 Arg Leu Trp Ile Thr Glu Lys Ala Gin Aia Phe Met Ser Ala 160 Glu Thr Asp Gly Phe 240 <210> 889 <211> 813 1223 WO 01/00843 WO 0100843PCTIiBOO/00923 <212> DNA <213> Corynebacterium giutamicum <2 <221> CDS <222> (101)..(790) <223> RXA02311 <400> 889 cattttgggt atcggttggt tgtccatcgg tggagctaag aataacctcc cactaaagct cctgggttag actcgaacgc ggcgctaagc atcgcagcca gtg gct aaa gca Val Ala Lys Ala 1 gat Asp 115 tta gac aag gac Leu Asp Lys Asp ttc gac gta gcg Phe Asp Val Ala tca Ser atg ttc gat gac Met Phe Asp Asp gtc gga Val Gly aag aac tac Lys Asn TIyr gtg tgg cga Val Trp Arg gat Asp ctc acc aat acc Leu Thr Asn Thr ctt tct ttt ggt Leu Ser Phe Gly cag gac cgt Gin Asp Arg cca ggg gag Pro Gly Giu aag cgc act agg cag cgc ctg gac ctc Lys Arg Thr Arg Gin Arg Leu Asp Leu aag gtg Lys Val ctt gat cta gct Leu Asp Leu Ala gga aca gcc gtt Gly Thr Ala Val acc gtg gag ttg Thr Val Glu Leu gca Ala aaa tcc ggc gcg Lys Ser Gly Ala tgt gtg gcg tgt Cys Val Ala Cys gat Asp ttc tcc cag ggc Phe Ser Gin Gly ctc gcc gca ggt Leu Ala Ala Gly gac cgc gat gtg.

Asp Arg Asp Val aag gtt gtg ggc Lys Val Val Gly gat ggc Asp Gly 100 atg cag ttg Met Gin Leu tat ggt ctg Tyr Gly Leu 120 ccg Pro 105 ttt gca gac aac agc ttt gat gct gtg Phe Ala Asp Asn Ser Phe Asp Ala Val 110 acc att tct Thr Ile Ser 115 aaa gaa atg Lys Giu Met cgc aat att cac Arg Asn Ile His gat Asp 125 ttc cgc gct ggc Phe Arg Ala Gly gcc cgc Ala Arg 135 gtg act aaa cct Val Thr Lys Pro ggt Gly 140 gga cgc ctc acc Gly Arg Leu Thr gcg gag ttc tcc Ala Giu Phe Ser acc ccc gtg atc cct Thr Pro Val Ile Pro gtg Val 155 ttc ggc acc gtg Phe Gly Thr Val aag gag tac ctc Lys Glu T'yr Leu cgc ctg ctg ccc Arg Leu Leu Pro cag Gin 170 gcg gcg cgc gca Ala Ala Arg Ala tcg tcc aac ccg Ser Ser Asn Pro gag gcc Giu Ala 180 tac att tac ctg gct gat tcc atc cgc gca tgg cct agc cag gcg gaa Tyr le Tyr Leu Ala Asp Ser Ile Arg Ala Trp, Pro Ser Gin Ala Giu 1224 WO 01/00843 WO 0100843PCTIBOOIOO923 185 190 195 cta gca cgg gag atc aac cag aat ggt tgg tca gat tgc ggt tgg cag 739 Leu Ala Arg Glu Ile Asn Gin Asn Gly Trp Ser Asp Cys Gly Trp Gin 200 205 210 aac ctg acc ttc ggc atc gtc gcg ctg cac tcg gcg att aaa cca gag 787 Asn Leu Thr Phe Gly Ilie Val Ala Leu His Ser Ala Ile Lys Pro Giu 215 220 225 aac tagtcgagtc ccacagaggg gag 813 Asn 230 <210> 890 <211> 230 <212> PRT <213> Corynebacteriun glutainicum <400> 890 Val Ala Lys Ala Asp Leu Asp Lys Asp Pro Phe Asp Val Ala Ser Met 1 5 10 Phe Asp Asp Val Gly Lys Asn Tyr Asp Leu Thr Asn Thr Val Leu Ser 25 Phe Gly Gin Asp Arg Val Trp, Arg Lys Arg Thr Arg Gin Arg Leu Asp 40 Leu Lys Pro Gly Glu Lys Val Leu Asp Leu Ala Ala Gly Thr Ala Val 55 Ser Thr Val Glu Leu Ala Lys Ser Gly Ala Phe Cys Val Ala Cys Asp 70 75 Phe Ser Gin Gly Met Leu Ala Ala Giy Lys Asp Arg Asp Val Ser Lys 90 Vai Vai Giy Asp Gly met Gin Leu Pro Phe Ala Asp Asn Ser Phe Asp 100 105 110 Ala Vai Thr Ilie Ser TIyr Gly Leu Arg Asn Ile His Asp Phe Arg Ala 115 120 125 Gly Leu Lys Giu Met Ala Arg Vai Thr Lys Pro Giy Gly Arg Leu Thr 130 135 140 Val Ala Glu Phe Ser Thr Pro Val Ilie Pro Val Phe Gly Thr Val Tyr 145 150 155 160 Lys Giu Tyr Leu Met Arg Leu Leu Pro Gin Ala Ala Arg Ala Val Ser 165 170 175 Ser Asn Pro Giu Ala Tyr Ilie Tyr Leu Ala Asp Ser Ile Arg Ala Trp 180 185 190 Pro Ser Gin Ala Giu Leu Ala Arg Giu Ilie Asn Gin Asn Gly Trp Ser 195 200 205 Asp Cys Gly Trp Gin Asn Leu Thr Phe Gly Ilie Val Ala Leu His Ser 1225 WO 01/00843 WO 0100843PCT/IB00100923 215 Ala Ile Lys Pro Glu Asn 225 230 <210> 891 <211> 876 <212> DN'A <213> Corynebacterium giutamicum <220> <221> <222> <223>

CDS

(101)..(853) R.XN02 912 <400> 891 catcggtgtc ggttcactga tggtggccaa gaaggttgga taacagggtt aatcttgggt gatattgaaa tgacgtattc ctgattgggc tgaaaaatct gtg aca tca cct gaa Val Thr Ser Pro Giu tta caa aac atc ctt aac aat tat tgg Leu Gin Asn Ile Leu Asn Asn Tyr Trp, ggc agg gca gag Gly Arg Ala Giu gct tac Ala TIyr cac ctc aac His Leu Asn tgg gaa aag Trp Giu Lys acc caa agc gag Thr Gin Ser Giu gca caa. ttt gaa Ala Gin Phe Giu cgc ccc atc Arg Pro Ile gtg tgg tcg aag gct ttg cct atc gtg tcg gaa gaa gcg Val Trp Ser Lys Ala Leu Pro Ile Val Ser Giu Giu Ala gta aag Vai Lys gtt ctc gat ctt Val Leu Asp Leu ggc Gly tgt ggc gct ggt Cys Gly Ala Gly gtc acc cac ctt Val Thr His Leu cta Leu agc gat tgc gga Ser Asp Cys Gly gaa aca atc ggc Giu Thr Ile Giy gat ggt tct gag Asp Giy Ser Giu atg atc aat caa Met Ile Asn Gin acg cag gag aat ggt ctt cgc agg tcg Thr Gin Giu Asn Gly Leu Arg Arg Ser acg ggt Thr Gly 100 cgg gcg act Arg Ala Thr cgg gaa ggc Arg Giu Gly 120 att ttt cag gtc Ile Phe Gin Val ggg Gly 110 gat gcg cat gat Asp Ala His Asp ccc gag ttc Pro Giu Phe 115 ttg tgg act Leu Trp, Thr 403 451 499 tct ttt gat gcg Ser Phe Asp Ala ata Ile 125 acc agc cgg tat Thr Ser Arg Tyr gtg Val1 130 ctg ctg Leu Leu 135 gat ccc cag gca Asp Pro Gin Ala att aat cgt tgg Ile Asn Arg Trp gtg Val1 145 tct ttg cta aaa Ser Leu Leu Lys cct ggt ggg gtt att gcg tgc gta gat gcg gct tgg tat ccg aag ggc Pro Gly Giy Vai Ilie Ala Cys Val ASP Ala Ala Trp Tyr Pro Lys Gly 1226 WO 01/00843 150 att gat gc Ile Asp A] gta gag ac Val Giu T1 acc tcc ac Thr Ser T1 2( aaa gaa gt Lys Giu Vz 215 cga ttt g Arg Phe G] 230 agg gga at Arg Gly I] ~t ggc La Gly tat Tyr 185 ggC Gly aca Thr ctc Leu aaa Lys acg Thr 170 acc Thr cac Hius ttg Leu tca Ser tcc Ser 250 155 gaa Glu ccg Pro aat Asn aca Thr c ca Pro 235 agt Ser 160 gta gat tca gtg gat ggt ccg Val Asp Ser Val Asp Gly Pro 175 gaa ctt ttg agg aat ctt ccc Giu Leu Leu Arg Asn Leu Pro 190 ttc gca gag ctt ttc cac aac Phe Ala Giu Leu Phe His Asn 205 210 ccc att gag ggg ctg gct gaa Pro Ile Giu Gly Leu Ala Giu 220 225 ggg cat gag tcg act ccg cag Gly His Giu Ser Thr Pro Gin 240 tagtgctgtt ttaagcggtc gag *PCTIIBOO/00923 165 agt gct ttc 643 Ser Ala Phe 180 atg tca acg 691 Met Ser Thr 195 gct ggc ctc 739 Ala Gly Leu ctt gac cag 787 Leu Asp Gin ttc cta ttc 835 Phe Leu Phe 245 876 <210> 892 <211> 251 <212> PRT <213> Corynebacterium giutainicum <400> 892 Val Thr Ser Pro Giu Leu Gin Asn Ile 1 Arg Phe Val1 Tyr Asp Arg His Tyr Val1 Ala Giu Ser Val Gly Arg Asp Val 130 Ser Glu Arg Giu Thr Ser Ser Pro 115 Leu Leu Leu Glu Lys Ser Ile Aia Glu Leu 135 Gly Asn Lys Val1 Asp Asn Thr Gly 120 Asp Gly Trp Arg Leu Gly Ile Asn Gly 110 Thr Asn Asp S er Ala Pro Ala G ly Gly Asp Ser Arg Ala 1227 WO 01/00843 145 Trp Tyr Pr Gly Pro Se Leu Pro Me 19 His Asn Al 210 Ala Giu Le 225 Pro Gin Ph Gly 165 Phe Thr Leu Gin Phe 245 Thr 170 Thr His Leu Ser Ser 250 155 Glu Val Asp Ser Pro Glu Leu Leu 190 Asn Phe Ala Glu 205 Thr Pro Ile Glu 220 Pro Gly His Glu 235 Ser PCTABOO/00923 160 Val Asp 175 Arg Asn Leu Phe Gly Leu Ser Thr 240 <210> 893 <211> 585 <212> DNA <213> Corynebacterium glutarnicum <22Q> <221> CDS <222> (101)..(562) <223> RXS00998 <400> 89 3 aatcggccta aaaggtgtga ccatttgggg gtagtggcga tgaaaatcca accattttag gccgactaga gtaattaatt gat Asp gct Ala ctc Leu gtg Val1 ttg Leu gtc Val ga t Asp gac Asp ctg Leu ctt Leu ggt Gly att Ile ctg Leu gaa G iu ggg Gly cac His gtC Val 75 tcC S er ttttaccagg tcacaggagg atg act tcc cgc gat met Thr Ser Arg Asp 1 ctt gcc gcc acc aga Leu Ala Ala Thr Arg aac gcc aat tat ggc Asn Ala Asn Tyr Gly atc gag ccc ggc cgg Ile Glu Pro Giy Arg ctg caa gtg gtg ggc Leu Gin Val Val Gly gaa tcc act gga tca Glu Ser Thr Gly Ser gtc gtc ggc atc aac Val Val Giy Ile Asn 100 115 163 211 259 307 355 403 1228 WO 01/00843 aac aac 8CC Asn Asn Thr PCTAIBOO/00923 gaa Glu gaa Glu atg Met 150 gca Ala tgc Cys 135 gtg Val1 gac ttc Asp Phe 105 ccg att Pro Ile cac cgc His Arg aac aag Asn Lys att tct gct gtg agc tcc ggt gtc atc Ilie Ser Ala Val Ser Ser Gly Val Ile 110 115 cag cta ggt ggc cgc acc cat ctg tgg Gin Leu Gly Gly Arg Thr His Leu Trp, 125 130 gga gaa gtC gtc gca cga acc aca ctg Gly Glu Vai Val Ala Arg Thr Thr Leu 140 145 tagccctata ctcgggcacc atg gtg gcc Val Ala cag atc Gin Ile cgc acc Arg Thr <210> 894 <211> 154 <212> PRT <213> Corynebacterium glutamicum <400> 894 Met Thr Ser Arg Asp Asp Gin Pro Gin Asp Leu Leu Ser Leu Ala Glu 1 Leu Asn Ile Leu Glu Val1 Gly His Thr 145 Ala Leu Val1 Leu 70 Val1 Asn Glu Giu met 150 Leu Asp Val1 Val Ala Asn Ala Cys 135 Val1 Leu Leu Val1 Tyr Ala Ser Leu Glu 140 Ala Tyr Ser Ala Gly Val1 110 Gly Val1 Leu Thr Lys Ile Lys Ser Arg Ala Asn Thr His Ala Met Ser Thr Arg <210> 895 <211> 1098 <212> DNA <213> Corynebacterium giutainicum <22 0> 1229 WO 01/00843 WO 0100843PCTIIBOO/00923 <221> CDS <222> (101)..(1075) <223> RX.A01215 tcgaaggctg ggtgcaaaag aagcgccctg gaaccgctgc cccaaaacgt ccacaaccag gaaggctaag caggatcctc agcacaagcc gcagaagccg atg act gct cac Met Thr Ala His tgg Trp aaa caa aac caa Lys Gin Asn Gin aac ctc atg ctg ttt tcg ggt cgt gcg Asn Leu Met Leu Phe Ser Gly Arg Ala cac cca His Pro gaa ctg gca Glu Leu Ala atg acg gca Met Thr Ala gct gta gct aaa Ala Val Ala Lys ctc gac gtc aac Leu Asp Val Asn gtc acc cca Val Thr Pro cgc gat ttc gcc Arg Asp Phe Ala ggt gaa atc tac gtc cgc ttc gag Gly Giu Ile Tyr Val Arg Phe Giu gaa tca Giu Ser gtt cgt ggc tcc Val Arg Gly Ser tgc ttc gtc ctg Cys Phe Val Leu tcc cac acc cag Ser His Thr Gin cct Pro ctc. aac aag tgg Leu Asn Lys Trp atg gaa cag ctg Met Giu Gin Leu c tg Leu atg atc gac gct Met Ile Asp Ala aag cgt ggt tcc Lys Arg Gly Ser aag cgc atc acc Lys Arg Ile Thr atc ctg ccg ttc Ile Leu Pro Phe tac cca Tyr Pro 100 tat gcc cgc Tyr Ala Arg cgc ctc atc Arg Leu Ile 120 cag Gin 105 gac aag aag cac Asp Lys Lys His ggc cgc gag cca Gly Arg Giu Pro att tct gct Ile Ser Ala 115 cgt atc gtg Arg Ile Val gcc gac ctc atg Ala Asp Leu Met ctc Leu 125 acc gct ggc gcg Thr Ala Gly Ala gac Asp 130 tcc gtg Ser Val 135 gac ttg cac acc Asp Leu His Thr gat Asp 140 cag atc cag ggc Gin Ile Gin Gly ttc Phe 145 ttc gac ggc cca Phe Asp Gly Pro gtc Val1 150 aac Asn gat cac atg cac Asp His Met His tac aac ctg gac Tyr Asn Leu Asp 170 atg ccg atc ctc Met Pro Ile Leu acc gat cac atc aag gaa Thr Asp His Ile Lys Giu 160 165 aac atc tgc gtg Asn Ile Cys Val tcc cct gac gca Ser Pro Asp Ala ggt cgc Gly Arg 180 gtg aag gtt Val Lys Val gca Ala 185 gag aag tgg gct Giu Lys Trp Ala aac Asn 190 acc ttg ggc gat Thr Leu Gly Asp gcc cca atg Ala Pro Met 195 gcg ttc gtg cac aag acc cgc tcc acc gag gta gca aac cag gtt gtc Ala Phe Val His Lys Thr Arg Ser Thr Giu Val Ala Asn Gin Val Val 1230 WO 01/00843 WO 0100843PCT/IBOO/00923 gcc aac Ala Asn .215 gac gac Asp Asp 230 ctg aag Leu Lys gtg ttc Val Phe gaa gtc Giu Val aac ctg 1027 Asn Leu 295 gag atc 1075 200 cgc Arg atg Met aag Lys tc t Ser atc Ile 280 acc Thr ttc 205 gtc Val1 Gly t ca Ser gag Giu ctg Leu 285 gca Ala gtc Val gac Asp acc Thr gtc Val cgc Arg 270 cca Pro ccg Pro acc Thr ggc Gly atc Ile gtc Val1 255 ctg Leu cag Gin ctg Leu acc Thr ctt Leu ggc Gly cac His 260 gct Ala tgg Trp atc Ile gag Giu ctc Leu gtc Val 245 ggt Gly gaa Glu agc Ser aac Asn gcc Ala 325 787 835 883 931 979 Giu Ile Phe Glu Asn Gly Ser taaacaccca tgcccaccac gga 1098 <210> 896 <211> 325 <212> PRT <213> Corynebacteriun giutamicum <400> 896 Met Thr Ala His Trp Lys Gin Asn Gin Lys 1 5 Gly Arg Ala His Pro Giu Leu Ala Giu Ala Val Asn Val Thr Pro met Thr Ala Arg Asp Tyr Vai Arg Phe Glu Glu Ser Val Arg Gly Gin Ser His Thr Gin Pro Leu Asn Lys Trp Met le. Asp Ala Leu Lys Arg Gly Ser Ala Leu Pro Phe Tyr Pro Tyr Ala Arg Gin Asp 1231 WO 01/00843 WO 0100843PCTIBOO/00923 100 105 110 Glu Pro Ile Ser Ala Arg Leu Ile Ala Asp Leu Met Leu Thr Ala Gly 115 120 125 Ala Asp Arg Ile Val Ser Val Asp Leu His Thr Asp Gln Ile Gln Gly 130 135 140 Phe Phe Asp Gly Pro Vai Asp His Met His Ala Met Pro Ile Leu Thr 145 150 155 160 Asp His Ile Lys Glu Asn Tyr Asn Leu Asp Asn Ile Cys Val Val Ser 165 170 175 Pro Asp Ala Gly Arg Val Lys Val Ala Giu Lys Trp, Ala Asn Thr Leu 180 185 190 Gly Asp Ala Pro Met Ala Phe Val His Lys Thr Arg Ser Thr Giu Val 195 200 205 Ala Asn Gin Val Val Ala Asn Arg Val Val Gly Asp Val Asp Gly Lys 210 215 220 Asp Cys Val Leu Leu Asp Asp Met Ile Asp Thr Gly Gly Thr Ile Ala 225 230 235 240 Gly Ala Val Gly Val Leu Lys Lys Ala Gly Ala Lys Ser Vai Val Ile 245 250 255 Ala Cys Thr His Gly Val Phe Ser Asp Pro Ala Arg Giu Arg Leu Ser 260 265 270 Ala Cys Gly Ala Glu Giu Val Ile Thr Thr Asp Thr Leu Pro Gin Ser 275 280 285 Thr Giu Gly Trp Ser Asn Leu Thr Val Leu Ser Ile Ala Pro Leu Leu 290 295 300 Ala Arg Thr Ile Asn Giu Ile Phe Glu Asn Gly Ser Val Thr Thr Leu 305 310 315 320 Phe Giu Gly Giu Ala 325 <210> 897 <211> 1470 <212> DNA <213> Corynebacterium giutaiicun <22 0> <221> CDS <222> (101)..(1447) <223> RXN00558 <400> 897 gaacaggcac cgcaggaaga atgcggcgtt ttcggcgttt gggcgccagg tgaggaagtc tcgaaactta cctactttgg cctcttcgca cttcagcacc gtg gtc aag aag ccc 115 Val Val Lys Lys Pro 1 1232 WO 01/00843 WO 0100843PCT/EBOO/00923 gcg ggc atc gca Ala Gly Ile Ala ggc gat ggc gaa Gly Asp Gly Giu atc ctg gtt ttc Ile Leu Val Phe aaa gat Lys Asp ttg ggc cta Leu Gly Leu cgc gga aac Arg Gly Asn tcc caa gtt ttc Ser Gin Val Phe caa cca att ctg Gin Pro Ile Leu gaa tcc ctc Glu Ser Leu atc gcc atc gga Ile Ala Ile Gly acc cga tac acc acc gcc ggc gga Thr Arg Tyr Thr Thr Ala Gly Gly aac acc Asn Thr tgg gaa aat gcc cag cct atg ttc cgc Trp, Glu Asn Ala Gin Pro Met Phe Arg gca cca gat ggc Ala Pro Asp Gly 307 355 acc Thr gat atc gcc ctt gga cac aac ggc aac ctg att aat tac atc Asp Ile Ala Leu Gly His Asn Gly Asn Leu Ile Asn Tyr Ile ttg ttg gac aaa Leu Leu Asp Lys acc gaa ctt ggc Thr Giu Leu Gly gtc gat ccc gcc Vai Asp Pro Ala aag aag Lys Lys 100 cca tca gat Pro Ser Asp gac gga aat Asp Gly Asn 120 acc Thr 105 gat gtg ctc act Asp Val Leu Thr gga Gly 110 ctg ctc gca agc Leu Leu Ala Ser ggc gtc cat Gly Val His 115 ccc agc gtcb Pro Ser Val aat ctc ttt gat Asn Leu Phe Asp tcc Ser 125 gcC aag gaa ctc Ala.Lys Giu Leu ctc Leu 130 aag gga Lys Gly 135 gcc tac tgc ctc Ala Tyr Cys Leu acc Thr 140 ttc acc gac gga Phe Thr Asp Gly cac His 145 acc ctg tac gca Thr Leu Tyr Ala gcg Ala 150 cgt gat cca ttc Arg Asp Pro Phe atc cgc cca ctg Ile Arg Pro Leu atc ggc cg Ile Gly Ar1 Cctc gag g Leu Giu 165 cgta ggt e Val Gly 180 547 595 643 cgc ggc tgg gta Arg Gly Trp Val gca tct gaa acc Ala Ser Giu Thr gcg ctc gac at( Ala Leu Asp Il gcc tcg cat Ala Ser His gaa tcc ggc Glu Ser Gly 200 gtg Val1 185 cgc gag gtc gaa Arg Giu Val Glu ggc gaa ctg att Gly Giu Leu Ile gct atc gac Ala Ile Asp 195 cgc aaa ggt Arg Lys Gly ctc aag tcc gca Leu Lys Ser Ala cga Arg 205 ttc gcc gag aca Phe Ala Giu Thr acc Thr 210 tgc gtc Cys Val 215 ttc gaa tac gtt Phe Glu Tyr Val tac Tyr 220 ctg gct cgt cca Leu Ala Arg Pro gac Asp 225 tcc gtg atc aag Ser Val Ile Lys gga Gly 230 aga aac gtc aac Arg Asn Val Asn gcc cga ctt gaa Ala Arg Leu Glu ggc cgc aag ctc Gly Arg Lys Leu 1233 WO 01/00843 WO 0100843PCTJIBOO/00923 gca gaa gca cca Ala Giu Ala Pro gca Ala 250 gtc ggc gat cta Val Gly Asp Leu ggc acc cca Gly Thr Pro ggc caa ggc Giy Gin Gly 280 gca Ala 265 a tg Met gca gte gga tcC Ala Val Gly Phe gcc Ala 270 tac Tyr gtc atc cca Val Ile Pro 255 caa gca tct Gin Ala Ser gte ggc cga Val Gly Arg acc Thr gtc aaa aac Val Lys Asn cca Pro gaa tca Giu Ser 260 cct tcc 1027 Pro Ser 295 teg cgc 1075 Leu Arg 310 atc gtc 1123 Ile Val gcc ggt 1171 Ala Gly tgg cca 1219 Trp Pro gcc aac 1267 Ala Asn 375 cgc tcc 1315 Arg Ser 390 atg gtt 1363 Met Val ttc gac 1411 Phe Asp gac cta 1457 Asp Leu gac act ctc cgc Asp Thr Leu Arg gag get atc gcc Giu Val Ile Ala 315 cgc ggt aac acc Arg Gly Asn Thr 330 caa Gin 300 gga atc cgc cc Giy Ile Arg Leu 305 ggc atc cca tc Giy Ile Pro Phe 275 acc ttc atc cag Thr Phe Ile Gin 290 aag ctg aac cca Lys Leu Asn Pro gtg gat gat tcc Val Asp Asp Ser 325 883 931 979 gga aag cgc cCC Gly Lys Arg Leu caa cgc gcc gtg Gin Arg Ala Vai 335 cac gea cgc atc His Val Arg Ile geg Vai 320 gt Val1 gca gce Ala Ala 345 egc tc Cys Phe gag gte Giu Val atc cgc atg ttg cgc gaa Ilie Arg Met Leu Arg Giu 340 gcc tca cca ccc gtg aaa Ala Ser Pro Pro Val Lys 355 acc cca ggc gaa ccc at Thr Pro Giv Giu Leu Ile 350 tac ggc aec gat ttt Tyr Gly Ilie Asp Phe 365 gcc Ala 360 370 gct gec acc age gac Ala Val Thr Ser Asp 380 gca atc ggc gca gac Ala Ile Gly Ala Asp 395 gca gca acc gag caa Ala Ala Thr Giu Gin 410 ggc aaa tac ccc atg Gly Lys Tyr Pro Met 425 aac gaa gca gaa atg gea gaa gca gtc Asn Giu Ala Giu Met Val Giu Ala Val 385 acc ctc ggc tac Thr Leu Gly Tyr 400 cca gcc aac gaa Pro Ala Asn Giu 415 ggt ctg cca cag Gly Leu Pro Gin gtc tcc atc gac tcc Val Ser Ile Asp Ser ctc tgc aec gcc tgc Leu Cys Ile Ala Cys 420 gga aac agc aac gca Gly Asn Ser Asn Ala 435 agt taagatcggt gtc Vai 440 cgc aag atg caa gca Arg Lys Met Gin Ala 445 430 acc gcc tca Thr Ala Ser Ser 1234 WO 01/00843 WO 0100843PCT/IBOO/00923 aggcgatagg ggt 1470 <210> 898 <211> 449 <212> PRT <213> Corynebacterium giutamicum <400> 898 Val Val Lys Lys Pro Ala Gly Ile Ala Val Gly Asp Gly Giu Gin Ile 1 5 10 Leu Val Phe Lys Asp Leu Gly Leu Val Ser Gin Val Phe Asp Gin Pro 25 Ile Leu Giu Ser Leu Arg Gly Asn Ile Ala Ile Gly His Thr Arg Tyr 40 Thr Thr Ala Gly Gly Asn Thr Trp Giu Asn Ala Gin Pro Met Phe Arg 55 Met Ala Pro Asp Giy Thr Asp Ile Ala Leu Gly His Asn Gly Asn Leu 70 75 Ile Asn Tyr Ile Giu Leu Leu Asp Lys Ala Thr Glu Leu Gly Leu Val 90 Asp Pro Ala Lys Lys Pro Ser Asp Thr Asp Val Leu Th3; Gly Leu Leu 100 105 110 Ala Ser Gly Val His Asp Gly Asn Asn Leu Phe Asp Ser Ala Lys Glu 115 120 125 Leu Leu Pro Ser Val Lys Gly Ala Tyr Cys Leu Thr Phe Thr Asp Gly 130 135 140 His Thr Leu Tyr Ala Ala Arg Asp Pro Phe Gly Ile Arg Pro Leu Ser 145 150 155 160 Ile Gly Arg Leu Glu Arg Gly Trp Val Vai Ala Ser Giu Thr Ala Ala 165 170 175 Leu Asp Ile Val Gly Ala Ser His Val Arg Giu Vai Giu Pro Gly Giu 180 185 190 Leu Ile Ala Ilie Asp Glu Ser Gly Leu Lys Ser Ala Arg Phe Ala Giu 195 200 205 Thr Thr Arg Lys Gly Cys Val Phe Glu Tyr Vai Tyr Leu Ala Arg Pro 210 215 220 Asp Ser Vai Ilie Lys Gly Arg Asn Val Asn Giu Ala Arg Leu Giu Ile 225 230 235 240 Gly Arg Lys Leu Ala Ala Glu Ala Pro Ala Val Gly Asp Leu Val Ile 245 250 255 Pro Thr Pro Giu Ser Gly Thr Pro Ala Ala Vai Gly Phe Ala Gin Ala 260 265 270 1235 WO 01/00843 WO 0100843PCTAIBOOIOO923 Ser Gly Ile Pro Phe Gly Gin Gly Met Val Lys Asn Ala Tyr Val Gly 275 280 285 Arg Thr Phe Ile Gin Pro Ser ASP Thr Leu Arg Gin Leu Gly Ile Arg 290 295 300 Leu Lys Leu Asn Pro Leu Arg Giu Val Ile Ala Gly Lys Arg Leu Val 305 310 315 320 Val Val Asp Asp Ser Ile Val Arg Gly Asn Thr Gin Arg Ala Val Ile 325 330 335 Arg Met Leu Arg Giu Ala Gly Ala Ala Giu Val His Val Arg Ile Ala 340 345 350 Ser Pro Pro Val Lys Trp Pro Cys Phe Tyr Gly Ile Asp Phe Ala Thr 355 360 365 Pro Gly Giu Leu Ilie Ala Asn Ala Val Thr Ser Asp Asn Giu Ala Giu 370 375 380 Met Val Giu Ala Val Arg Ser Ala Ile Gly Ala Asp Thr Leu Gly Tyr 385 390 395 400 Val Ser Ile Asp Ser Met Val Ala Ala Thr Giu Gin Pro Ala Asn Giu 405 410 415 Leu Cys Ilie Ala Cys Phe Asp Gly Lys Tyr Pro Met Gly Leu Pro Gin 420 425 430 Gly Asn Ser Asn Ala Asp Leu Val Arg Lys Met Gin Ala Thr Ala Ser 435 440 445 Ser <210> 899 <211> 524 <212> DNA <213> Corynebacteriun glutamicun <220> <221> CDS <222> (61)..(501) <223> FRXA00558 <400> 899 gggcgccagg tgaggaagta tcgcagctca cctactttgg cctcttcgca gttcagcacc gtg gtc aag aag ccc gcg ggc atc gca gta ggc gat ggc gaa cag atc 108 Val Val Lys Lys Pro Ala Gly Ilie Ala Vai Gly Asp Gly Giu Gin Ile 1 5 10 ctg gtt ttc aaa gat ttg ggc cta gtc tcc caa gtt ttc gac caa cca 156 Leu Val Phe Lys Asp Leu Gly Leu Val Ser Gin Val Phe Asp Gin Pro 25 att ctg gaa tcc ctc cgc gga sac atc gcc atc ggs cac acc cga tsc 204 Ilie Leu Giu Ser Leu Arg Gly Asn Ilie Ala Ile Gly His Thr Arg Tyr 1236 WO 01/00843 WO 0100843PCTIIBOO/00923 acc Thr atg Met att Ile gat Asp gca Ala ctc Leu acc Thr gca Ala aat Asn ccc Pro agc Ser ctc Leu 130 ggc Gly ga t Asp atc Ile gga Gly ggc Gly gag Giu aac acc Asn Thr 55 acc gat Thr Asp 70 ttg ttg Leu Leu gaa Glu gcc Ala aaa Lys gcc Ala gga Gly 75 acc Thr gtg Val1 ttt Phe ctc Leu cag Gin 60 cac His gaa Glu ctc Leu gat Asp acc Thr 140 cc t Pro aac Asn ctt Leu act Thr tcC Ser 125 t tc Phe ttc cgc Phe Arg aac ctg Asn Leu ctc gtc Leu Val ctg ctc Leu Leu aag gaa Lys Giu gac gga Asp Gly aag aag cca tca gat acc gat Lys Lys Pro Ser Asp Thr Asp 100 105 gtc cat gac gga aat aat ctc Val His Asp Gly Asn Asn Leu 120 agc gtc aag gga gcc tac tgc Ser Val Lys Gly Ala Tyr Cys 135 cac acc ctg taagcagcgc gtgatccat His Thr Leu 145 <210> 900 <211> 147 <212> PRT <213> Corynebacterium glutamicum <400> 900 Val Val Lys Lys Pro Ala Gly Ile 1 5 Leu Val Phe Lys Asp Leu Gly Leu N Ile Leu Glu Ser Leu Arg Gly Asn J 40 Thr Thr Ala Gly Gly Asn Thr Trp C Met Ala Pro Asp Gly Thr Asp Ile .1 70 Ile Asn Tyr Ile Glu Leu Leu Asp L Asp Pro Ala Lys Lys Pro Ser Asp 'I 100 1 Ala Ser Gly Val His Asp Gly Asn P~ 115 120 -Cgg Val1 10 Ser Ala Asn Leu Ala 90 Asp Leu Gly Gin Ile Ala Gly 75 Thr Val1 Phe Gly Phe His Pro Asn Leu Thr Ser 125 Gin Gin Arg Phe Asn Leu Leu Lys Ile Pro Tyr Arg Leu Val Leu Glu 1237 WO 01/00843 WO 0100843PCTJIBOO/00923 Leu Leu Pro Ser Val Lys Gly Ala Tyr Cys Leu Thr Phe Thr Asp Gly 130 135 140 His Thr Leu 145 <210> <211> <212> <213> <220> <221> <222> <223> 901 1386

DNA

Corynebacteriun

CDS

(101)..(1363) RXN00626 glutamicui <400> 901 tcattttaag gcgcttttcg acgccacttt caaccatttc cgaaccgcca agaatactgg aatagcttgg atcaagtttt gcaggataaa ctgtgcaacc atg cgc att ctg gta Met Arg Ile Leu Val atc ggc tcg ggc Ile Gly Ser Gly cgt gag cac gcc ctc ctc cgt gga ctg Arg Glu His Ala Leu Leu Arg Gly Leu tca act Ser Thr gac cct gca Asp Pro Ala gga tcg atc Gly Ser Ile acc *Thr act gaa ctc cac Thr Glu Leu His gcc cca ggt aac Ala Pro Gly Asn gct ggt ctt Ala Gly Leu gat cca gag Asp Pro Glu gca act gtc cac cct ggc atc aag gct Ala Thr Val His Pro Gly Ile Lys Ala gct gtc Ala Val act gcg ttg gct Thr Ala Leu Ala gaa ctg aac tct Giu Leu Asn Ser ctg gtt gtt atc Leu Val Val Ile 259 307 355 403 ggc Gly cca gag atc cct Pro Glu Ile Pro ttg Leu gtt gcg ggt gtt Val Ala Gly Val gat gca ctt cgc Asp Ala Leu Arg gcg ggt atc gct Ala Gly Ile Ala ttt ggt cct aac Phe Gly Pro Asn gac gct gct cgt Asp Ala Ala Arg atc gag Ile Glu 100 ggc tcc aag Gly Ser Lys act gct cat Thr Ala His 120 ttc gct aag gat Phe Ala Lys Asp gtc Val1 110 atg gct gcg cag Met Ala Ala Gin ggt gtt cgt Gly Val Arg 115 gct gag act att Ala Glu Thr Ile act Thr 125 cct ggt gcg tct tct gaa gat att Pro Gly Ala Ser Ser Giu Asp Ile 130 gat Asp gct gcc ata gat cgt Ala Ala Ile Asp Arg 135 ggc cca acg tgg gtt Gly Pro Thr Trp, Val 145 gtc aag gat gat Val Lys Asp Asp ggt ttg tct gcg ggc aag ggt gtt gtg gtt acc ccc gat cgt gca. gca 1238 WO 01/00843 Gly Leu Se 150 gca cgt gc Ala Arg Al ctg gag tc Leu Glu Se gat ggc ga Asp Gly Gl gcg tac ga Ala Tyr As 215 gcg ccg ct Ala Pro Le 230 gag gtc tg Glu Val Cy tac tcc gg Tyr Ser Gl gca gta gt Ala Val Va 28 gta ctg gc 1027 Val Leu Al 295 gct act gg 1075 Ala Thr Gi 310 tac gcc ct 1123 Tyr Ala Le cgt act gg 1171 Arg Thr Gl gct ggt ac 1219 r t a

C

r g

U

0

C

p t u

C

'5 t y g 1 0 a a a y g

U

t y c Ala Gly cac gta Hius Val 170 ttc ctt Phe Leu 185 acg gta Thr Val aac gat Asn Asp cc t Pro gt t Val1 ctg Leu 265 gag Glu cta Leu acc Thr act Thr gat Asp 345 gca tgg Trp cct Pro 250 ctt Leu t tc Phe ctg Leu t tg Leu gtg Val 330 gtc Val c tc Lys 155 ga t Asp gat Asp gtt Val gag Glu ctg Leu 235 gtt Val1 tac Tyr aac Asn aag Lys gca Ala 315 gtg Val atc Ile aat Gly gca Ala ggc Gly cct Pro ggc Gly 220 cct Pro gct Ala gca Ala tgc Cys act Thr 300 gag Glu ttg Leu cgc Arg gct Val1 gtg Val1 cc t Pro c tg Leu 205 cca Pro gaa Glu cgt Arg ggt Gly cgc Arg 285 cct Pro cag Gln gc t Ala aac Asn gaa Glu 365 Val ctt Leu gag Glu 190 ctg Leu aa c Asn gat Asp gag Glu atc Ile 270 ttc Phe cta Leu cca Pro tct Ser gct Ala 350 99C Val1 gag Glu 175 gtt Val cca Pro act Thr ggc Gly atg Met 255 gca Ala ggc Gly gca Ala gca Ala tac Tyr 335 gat Asp gag Thr 160 ggc Gly tcc Ser gcg Ala ggt Gly gtc Val 240 gtg Val tgg Trp, gat Asp gta Val ctg Leu 320 aac Asn gca Ala ctg Pro gga Gly ctg Leu cag Gln ggc Gly 225 cag Gln gca Ala ggt Gly cba Pro ctg Leu 305 gag Glu tac Tyr gat Asp gtc Asp aat Asn ttc Phe gat Asp 210 atg Met cgc Arg cgt Arg gca Ala gaa Glu 290 ctc Leu tgg Trp cca Pro aac Asn tct Ser 370 Arg cca Pro tgc Cys 195 cac His gg t Gly at t Ile ggt Gly gaa Glu 275 acc Thr aac Asn gag Glu gag Glu gtt Val1 355 gcg PCTJIBOO/00923 La Ala 165 :t ttg 643 3d Leu :g gtt 691 au Val 5Lg cgt 739 r's Arg :t tat 787 La Tyr -c gat 835 il Asp 245 Ic gcg 883 (s Ala Ic cct 931 Ly Pro Ig gct 979 Ln Ala gca Ala gat Asp gca Ala 340 ctt Leu 99C gt t Val gct Ala 325 cc t Pro ca c His ggt Ala Gly Thr 360 Ala Leu Asn Ala Gly Glu Leu Val Ala Gly Gly 1239 WO 01/00843 cgt gtt ctt aac gtg atc ggt gtg ggt 1267 Arg Val Leu Asn Val Ile Gly Val Gly 375 380 gat aac gcg tac acc acc atc aag gac 1315 Asp Asn Ala Tyr Thr Thr Ile Lys Asp 390 395 tac cgc agc gat atc gca ttg gct gca 1363 Tyr Arg Ser Asp Ile Ala Leu Ala Ala 410 taaaagcagt acgcagatag gct 1386 <210> 902 <211> 421 <212> PRT <213> Corynebacterium glutarnicum <400> 902 Met Arg Ile Leu Val Ile Gly Ser Gly gag Glu at t Ile tta Leu 415 acc ctg gag gct Thr Leu Glu Ala 385 gaa ctt gag gga Glu Leu Glu Gly PCTIBOO/00923 gca cgc Ala Arg agc cac Ser His 405 tcg atc Ser Ile 420 400 gag Glu ggt cgt atc Gly Arg Ile 1 Arg Gly Ala Asp Asp Ala Ala Ser Val 145 Pro Gly Ser Gly Pro Val1 Arg Ile 100 Val1 Asp Asp Ala Val1 180 Ala Ile Thr Glu Ile Lys His 120 Ala Ser Ala Ser Thr 25 Ala Ala Ile Ala Ala 105 Ala Ile Ala His Phe 185 Ala 10 Thr Thr Leu Pro Val Phe Glu Asp Gly Val 170 Leu Arg Glu Val Ala Leu 75 Phe Ala Thr Arg Lys 155 Asp Asp Glu Leu His Lys Val1 Gly Lys Ile Phe 140 Gly Ala Gly His His Pro Glu Ala Pro Asp Thr 125 Gly Val1 Val1 Pro Ala Val1 Gly Leu Gly Asn Val1 110 Pro Pro Val1 Leu Glu 190 Leu Ala Ile Asn Val1 Lys Met Gly Thr Val Glu 175 Val1 Leu Pro Lys Ser Ala Asp Ala Ala Trp Thr 160 G ly Ser 1240 WO 01/00843 PCT/EBOO/00923 Leu Val Asp Gly Glu Thr Val Val Pro Leu Leu Pro Ala 200 205 Leu Gin Gly 225 Gin Ala Gly Pro Leu 305 Glu Tyr Asp Val1 Leu 385 Leu Gly Phe Asp 210 Met Arg Arg Ala Glu 290 Leu Trp, Pro Asn Ser 370 Giu Glu Arg Cys 195 His Gly Ile Gly Glu 275 Thr Asn Glu Glu Val 355 Ala Ala Gly Ile Lys Arg Ala Tyr Val Asp 245 Cys Ala 260 Gly Pro Gin Ala Ala Val Asp Ala 325 Ala Pro 340 Leu His Gly Gly Ala Arg Ser His 405 Ser Ile 420 Asp Leu Cys Gly Val 280 Ala Gly Leu Gly Thr 360 Leu Ala Asn Pro Val Leu 265 Glu Leu Thr Thr Asp 345 Ala Asn Tyr Asp Trp Pro 250 Leu Phe Leu Leu Val 330 Val Leu Val1 Thr Glu Leu 235 Val1 Tyr Asn Lys Ala 315 Val Ile Asn Ile Thr 395 Gly 220 Pro Ala Ala Cys Thr 300 Giu Leu Arg Ala Gly 380 Ile Asn Asp Giu Ile 270 Phe Leu Pro Ser Ala 350 Gly Gly Asp Thr Gly Met 255 Ala Gly Al a Ala Tyr 335 Asp Giu Glu Ile G ly Val1 240 Val Trp Asp Val1 Leu 320 Asn Ala Leu Thr Glu 400 Arg Ser Asp Ile Ala Leu Ala Ala Leu Giu <210> 903 <211> 364 <212> DNA <213> Corynebacteriun glutaxnicum <22 0> <221> CDS <222> (101)..(364) <223> FRXA00629 <400> 903 tcattttaag gcgcttttcg acgccacttt caaccatttc cgaaccgcca agaatactgg aatagcttgg atcaagtttt gcaggataaa ctgtgcaacc atg cgc att ctg gta 115 Met Arg Ile Leu Val 1241 WO 01/00843 atc Ile gac Asp gga Gly gct Ala ggC Gly gcg Ala ggc Gly acc Thr gca Ala gcg Ala atc Ile gcc Ala act Thr act Thr t tg Leu cc t Pro ctc Leu cca Pro aag Lys tc t Ser gct Ala 80 cgt Arg ggt Gly gct Ala ga t Asp ga t Asp PCTJIBOO/00923 ca act .163 er Thr gt ctt 211 ly Leu ca gag 259 ro Glu tt atc 307 al Ile gc gcg 355 rg Ala <210> 904 <211> 88 <212> PRT <213> Corynebacterium glutaxnicun <400> 904 Met Arg Ilie Leu Val Ile Gly. Ser 1 5 Arg Gly Leu Ser Thr Asp Pro Ala Gly Asn Ala Gly Leu Gly Ser Ile 40 Ala Asp Asp Pro Glu Ala Val Thr 55 Asp Leu Val Val Ile Gly Pro Glu 70 Asp Ala Leu Arg Ala Ala Gly Ile Gly Ala Arg Glu His Ala Leu 10 Thr Thr Glu Leu His Val Ala 25 Ala Thr Val His Pro Gly Ile Ala Leu Ala Lys Glu Leu Asn Ile Pro Leu Val Ala Gly Val <210> 905 <211> 803 <212> DNA <213> Corynebacterium glutainicum <22 0> <221> CDS <222> <223> FRXA00626 1242 WO 01/00843 <400> 905 PCT/IBOO/00923 gat Asp 1 cgt gca gca Arg Ala Ala cgt gct cac gta Arg Ala His Val gca gtg ctt gag Ala Val Leu Glu ggc gga Gly Gly aat cca gtt Asn Pro Val ttc ttc ctg Phe Phe Leu ctg gag tcc ttc Leu Glu Ser Phe ctt gat ggc cct gag gtt tcc ctg Leu Asp Gly Pro Glu Val Ser Leu gtt gat ggc gag acg gta gtt cct ctg Val Asp Gly Glu Thr Val Val Pro Leu cca gcg cag Pro Ala Gin gat cac Asp His aag cgt gcg tac Lys Arg Ala Tyr aac gat gag ggc Asn Asp Glu Gly aac act ggt ggc Asn Thr Gly Gly atg Met ggt gct tat gcg Gly Ala Tyr Ala ctt cct tgg ctg Leu Pro Trp Leu gaa gat ggc gtc Glu Asp Gly Val cgc att gtc gat Arg Ilie Val Asp gtc tgc gtt cct Val Cys Val Pro gct cgt gag atg Ala Arg Glu Met gtg gca Val Ala cgt ggt tgc Arg Gly Cys gca gaa ggc Ala Glu Gly 115 tac tcc ggt ctg Tyr Ser Gly Leu ctt Leu 105 tac gca ggt atc Tyr Ala Gly Ile gca tgg ggt Ala Trp, Gly 110 ggc gat cca Gly Asp.Pro cct gca gta gtg Pro Ala Val Val gag Glu 120 ttc aac tgc cgc Phe Asn Cys Arg gaa acc Glu Thr 130 cag gct gta ctg Gin Ala Val Leu gca Ala 135 cta ctg aag act Leu Leu Lys Thr cta gca gta ctg Leu Ala Val Leu ctc Leu 145 aac gca gtt gct Asn Ala Val Ala gga acc ttg gca Gly Thr Leu Ala cag cca gca ctg Gin Pro Ala Leu 432 480 528 tgg gag gat gct Trp Glu Asp Ala gcc ctg act gtg Ala Leu Thr Val ttg gct tct tac Leu Ala Ser Tyr aac tac Asn Tyr 175 cca gag gca Pro Glu Ala aac gtt ctt Asn Val Leu 195 cgt act ggt gat Arg Thr Gly Asp atc cgc aac gct Ile Arg Asn Ala gat gca gat Asp Ala Asp 190 gag ctg gtc Glu Leu Val cac gct ggt acc His Ala Gly Thr gca Ala 200 ctc aat gct gaa Leu Asn Ala Glu tct gcg Ser Ala 210 ggc ggt cgt gtt Gly Gly Arg Val ctt Leu 215 aac gtg atc ggt Asn Val Ilie Gly ggt gag acc ctg Gly Glu Thr Leu 672 720 gag Glu 225 gct gca cgc gat Ala Ala Arg Asp gcg tac acc acc Ala Tyr Thr Thr aag gac att gaa Lys Asp Ile Glu ctt Leu 240 1243 WO 01/00843 WO 0100843PCTIIBO0/00923 gag gga agc cac tac cgc agc gat atc gca ttg gct gca tta gag ggt 768 Giu Gly Ser His Tyr Arg Ser Asp Ilie Ala Leu Ala Ala Leu Giu Gly 245 250 255 cgt atc tcg atc taaaagcagt acgcagatag gct 803 Arg Ilie Ser Ile 260 <210> 906 <211> 260 <212> PRT <213> Corynebacteriun glutamicun <400> 906 Asp Arg Ala Ala Ala Arg Ala His Val Asp Ala Val Leu Giu Gly Gly 1 5 10 Asn Pro Val Leu Leu Giu Ser Phe Leu Asp Gly Pro Giu Val Ser Leu 25 Phe Phe Leu Val Asp Gly Giu Thr Val Val Pro Leu Leu Pro Ala Gin 40 Asp His Lys Arg Ala Tyr Asp Asn Asp Giu Gly Pro Asn Thr Gly Gly 55 Met Gly Ala Tyr Ala Pro Leu Pro Trp, Leu Pro Giu Asp Gly Val Gin 70 .75 Arg Ilie Val Asp Giu Val Cys Val Pro Val Ala Arg Giu Met Val Ala 90 Arg Gly Cys Ala Tyr Ser Gly Leu Leu Tyr Ala Gly Ile Ala Trp Gly 100 105 110 Ala Giu Gly Pro Ala Val Val Giu Phe Asn Cys Arg Phe Gly Asp Pro 115 120 125 Giu Thr Gin Ala Val Leu Ala Leu Leu Lys Thr Pro Leu Ala Val Leu 130 135 140 Leu Asn Ala Val Ala Thr Gly Thr Leu Ala Giu Gin Pro Ala Leu Giu 145 150 155 160 Trp Giu Asp Ala Tyr Ala Leu Thr Val Val Leu Ala Ser Tyr Asn Tyr 165 170 175 Pro Giu Ala Pro Arg Thr Gly Asp Val Ile Arg Asn Ala Asp Ala Asp 180 185 190 Asn Val Leu His Ala Gly Thr Ala Leu Asn Ala Giu Gly Giu Leu Val 195 200 205 Ser Ala Gly Gly Arg Val Leu Asn Val Ile Gly Val Gly Giu Thr Leu 210 215 220 Giu Ala Ala Arg Asp Asn Ala Tyr Thr Thr Ile Lys Asp Ile Giu Leu 225 230 235 240 1244 WO 01/00843 WO 0100843PCTIIBOO/00923 Glu Gly Ser His Tyr Arg Ser Asp Ile Ala Leu Ala Ala Leu Glu Gly 245 250 255 Arg Ile Ser Ile 260 <210> 907 <211> 714 <212> DNA <213> Corynebacterium glutanicun <220> <221> <222> <223>

CDS

(691) RXA02 623 <400> 907 aatcctgaag aggaagaatc cgacgaagaa attgagacag aaactgaggc tgaagaaacc aatgatggtt ccgaggccga agaccgttaa catatctgtt gtg aat tct gac tct Val Asn Ser Asp Ser acg acc acc att gtc gtg cta gct tcc gga aca ggc acc ctc Thr Thr Thr Ile Val Val Leu Ala Ser Gly Thr Gly Thr Leu ctt cag Leu Gin tca ctc att Ser Leu Ile gcg caa ggt acc Ala Gin Gly Thr tcg atc gtg ggc Ser Ile Val Gly gtt gtc tct Val Val Ser ggt att gat Gly Ile Asp gac gtc gaa tgc cct gca ctt Asp Vai Giu Cys Pro Ala Leu tcc aga gcc gca Ser Arg Ala Ala gat cgt gca Asp Arg Ala gat gca Asp Ala act gca Thr Ala gtt gtt ccg ctt Val Val Pro Leu gga aaa Gly Lys 60 cag Gin tgg aac cac gag Trp Asn His Glu 259 3 07 355 ctt Leu gca gac gca gtt Ala Asp Ala Val gta agc gac cca gat ttg gtg gtc tct Val Ser ASP Pro Asp Leu Val Val Ser 80 gcg Ala gga ttc atg aaa Gly Phe Met Lys ttg ggc gaa ggt Leu Gly Glu Gly ctc tca agg ttc Leu Ser Arg Phe ccg tcc Pro Ser 100 cgc atc atc Arg Ile Ile cac gcg gtt His Ala Val 120 aac Asn 105 acc cac cca gct Thr His Pro Ala t ta Leu 110 ttg cct tct ttC cct ggt gcc Leu Pro Ser Phe Pro Gly Ala 115 cgc gat gct ttg Arg Asp Ala Leu gca Ala 125 tac ggt gtg aaa Tyr Gly Val Lys tca ggt tcg Ser Gly Ser aca gtt Thr Val 135 cac ctt gtc gat His Leu Val Asp ggt gtg gat acc Gly Val Asp Thr ggc Gly 145 cca att att gct Pro Ile Ile Ala caa cga gca gtg ccg gta gaa gtg aat gat gat gaa tcc agc ctg cat 1245 WO 01/00843 Gin Arg Ala Val Pro Val Glu Val Asn 150 155 gaa aga atc aag cag gtt gag cgt aaa Glu Arg Ile Lys Gin Val Glu Arg Lys 170 agc gtg gaa ttt tcg cgt cag ggt ggc Ser Val Glu Phe Ser Arg Gin Giy Gly 185 190 taaatccttc atgagcgatg atc <210> 908 <211> 197 <2i2> PRT <2i3> Corynebacterium giutamicum <400> 908 Vai Asn Ser Asp Ser Thr Thr Thr Ile PCTIIBOO/00923 Asp Asp Glu Ser Ser Leu His 160 165 ctc att gta gaa gtc ctg aac 643 Leu Ile Vai Giu Val Leu Asn 175 180 gta caa ctc aac tgg aga ggc 691 Val Gin Leu Asn Trp Arg Gly 195 714 1 Gly Thr Leu Val Gly Val Asp Ala Gly Gin Trp Asn Leu Val Val Ser Arg Phe Ser Phe Pro 115 Lys Val Ser 130 Gly Pro Ile 145 Glu Ser Ser Val Glu Val Leu Asn Trp 195 <210> 909 Ser Asp Thr Leu 70 Gly Arg His Thr Gin 150 Glu Ser Val 10 Ala Pro Pro Val Ile Thr Asp Val Pro Gin 170 Ser Val Gin Ala Leu Ala Leu His Ala Asp Va1 155 Val Arg Ala Thr Ser Lys Ser Glu Ala Ala 125 Gly Val Arg Gly Ser Tyr Arg Asp Asp Gly Leu 110 Tyr Va1 Asn Lys Gly 190 Gly Ser Ala Arg Pro Phe Leu Gly Asp Asp Leu 175 Val 1246 WO 01/00843 PCTIiBOO/00923 <211> 1347 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (101)..(1324) <223> RXA01442 <400> 909 aattaagcaa cctttttaac aggtttagag ctttcacgag gtggcgtcag ataaagtgaa caccaatatt tctgacatct tccaacggag gctaaaaggc atg tac atc cca gag 115 Met Tyr Ile Pro Giu tcg Ser gga Gly ggC Gly cag Gin gtg Vai atc Ilie cta Leu cgc Arg tcc Ser gaa Giu 150 Gly acc Thr gaa Giu gtc Val1 cac His ttg Leu ctg Leu atc Ile 105 atc Ile gag Giu ggt Gly caa Gin cct Pro t ta Leu cat His ttc Phe gtg Val gca Ala g tg Val cgc Arg ttc Phe tac Tyr tc t Ser 170 ttg Leu ggc Giy gca Ala tcc Ser gag Giu acc Thr ccc Pro aag Lys tgc Cys ccc Pro 155 gtt Val 1 aaa Lys gct Ala cat His aca Thr tt t Phe atc Ile aag Lys ctg Leu t tc Phe 145 gtg Val ga t Asp gtg Val t tc Phe gcc Ala gat Asp gtc Val gaa Giu ctg Leu ggt Gly 130 tcc Ser atg Met ctg Leu atg Met cag Gin cca Pro gca Ala att Ile gaa Giu ac c Thr 115 ctt Leu gca Ala agt Ser cag Gin tgg gat tat gcg atg agc ggt gca cgc gtg gcc aac tcc cgc gtc atc 1247 WO 01/00843 Trp Asp Tyr gtg gaa gct Val Glu Ala 200 agg tcc atc Arg Ser Ile 215 ccc att ggg Pro Ile Gly 230 cca atg gag Pro Met Glu gca cgc atc Ala Arg Ile ctc ttt gtc Leu Phe Val 280 cca cac gac 1027 Pro His Asp 295 ttt gaa ctc 1075 Phe Glu Leu 310 ctg att tct 1123 Leu Ile Ser gaa ggc gtg 1171 Glu Gly Val act gat ctt 1219 Thr Asp Leu 360 atg ggt gtt 1267 Met Gly Val 375 cgc gcc act 1315 Ala 185 ttc Phe gat Asp cac His atg Met acc Thr 265 t cc Ser ac c Thr cac His cca Pro agc Ser 345 cgt Arg gca Ala ttg Met gtg Val ccc Pro cgc Arg act Thr 250 aac Asn ggc Gly ggc Gly gcc Ala ggt Gly 330 tac Tyr atc Ile gtg Val gct Ser gaa Glu acc Thr caa Gin 235 cc t Pro gca Ala gat Asp ctt Leu aag Lys 315 gcc Ala acc Thr ttt Phe tcc Ser gcc Gly t tc Phe acc Thr 220 gaa Giu cgc Arg t tg Leu gac Asp gtc Val 300 gca Al a tcc Ser ggg Gly gcc Ala acc Thr 380 gcg Ala ga t Asp 205 tc t Ser gac Asp gcg Ala ggc Gly gtg Val 285 acc Thr att Ile gc t Ala t tg Leu aag Lys 365 gct Ala gcg Arg 190 tac Tyr aag Lys ggc Gly ctg Leu gga Giy 270 tac Tyr ctt Leu ctg Leu gtc Val gct Ala 350 cca Pro gag Glu atc Val gag Glu cct Pro gac Asp gaa Glu 255 cgc Arg ttc Phe gcc Ala gga Gly atc Ile 335 gaa Glu gag Glu gat Asp aag Lys Ala atc Ile gcg Ala tac Tyr 240 aac Asn ggc Gly tc t Ser acc Thr ttg Leu 320 tac Tyr gcg Ala gcc Ala gtg Val gtt Val 400 Asn acc Thr acc Thr 225 gtg Val gca Ala gta Val gaa Glu cag Gin 305 cct Pro gg t Gly ctg Leu ttc Phe gc t Ala 385 cat His Ser ctg Leu 210 tgg Trp gaa Glu cgc Arg ttt Phe gtc Val1 290 cgt Arg gtt Val ggc Gly gca Ala acc Thr 370 gca Ala cca Pro Arg 195 t tg Leu ttc Phe tcc Ser tca Ser gg t Gly 275 t cc Se r ttc Phe gat Asp atc Ile gtg Val1 355 aag Lys gcc Ala gga Gly PCTJIBOO/00923 Val Ile aca gta 739 Thr Val tgt gag 787 Cys Giu tgg cag 835 Trp Gin 245 gta gcc 883 Val Ala 260 gtg gag 931 Val Glu cca cgc 979 Pro Arg tct gaa Ser Giu gtc acc Val Thr 325 gaa tct Giu Ser 340 gct gaa Ala Glu cgt cgc Arg Arg agg gac Arg Asp aat tcc Asn Ser 405 Arg Ala 390 Thr Leu Ala Ala Ala Ala Ile 395 1248 WO 01/00843 WO 0100843PCTIBOO/00923 gca gag gct taacatgctg ggaaagcatc ggg 1347 Ala Glu Ala <210> 910 <211> 408 <212> PRT <213> Corynebacteriun glutamicun <400> 910- Met Tyr Ilie Pro Glu Ser Ile Gly Thr Pro Leu Thr Pro Asn Ala Thr 1 5 10 Lys Val Met Leu Leu Gly Ser Gly Glu Leu Gly Lys Glu Val Ala Ile 25 Ala Phe Gln Arg Leu Gly Leu Glu Val His Ala Val Asp Arg Tyr Glu 40 His Ala Pro Ala His Gln Val Ala His Phe Ser Tyr Val Ile Asp Met 55 Thr Asp Ala Ala Gin Val Arg Glu Leu Val Giu Arg Val Arg Pro Asp 70 75 Phe Val Ilie Pro Glu Ile Giu Ala Leu Ala Thr Asp Glu Leu Val Lys 90 Ilie Giu Giu Giu Gly Leu Ala Thr Ile Val Pro Thr Ala Arg Ala Ala 100 105 Lys Leu Thr Met Asn Arg Giu Gly Ile Arg Lys Leu Ala Ala Glu Glu 120 125 Leu Gly Leu Pro Thr Ser Asn Tyr Glu Phe Cys Ser Thr Phe Giu Giu 130 135 140 Phe Ser Ala Ala Ala Glu Lys Leu Gly Tyr Pro Asn Val Val Lys Pro 145 150 155 160 Val Met Ser Ser Ser Gly Lys Gly Gin Ser Val Leu Arg Ser Ser Asp 165 170 175 Asp Leu Gln Ala Ala Trp Asp Tyr Ala Met Ser Gly Ala Arg Val Ala 180 185 190 Asn Ser Arg Val Ile Val Glu Ala Phe Val Giu Phe Asp Tyr Giu Ile 195 200 205 Thr Leu Leu Thr Val Arg Ser Ile Asp Pro Thr Thr Ser Lys Pro Ala 210 215 220 Thr Trp Phe Cys Giu Pro Ile Gly His Arg Gin Glu Asp Gly Asp Tyr 225 230 235 240 Val Giu Ser Trp Gln Pro Met Glu Met Thr Pro Arg Ala Leu Giu Asn 245 250 255 1249 WO 01/00843 Ala Arg Ser Val Phe Giy 275 Giu Val Ser 290 Gin Arg Phe 305 Pro Val Asp Gly Gly Ile Leu Ala Val 355 Phe Thr Lys 370 Ala Ala Ala 385 His Pro Gly Ala Leu Pro Phe 310 Leu Giu Thr Met Arg 390 Al a Arg Phe His 295 Giu Ile Gly Asp Gly 375 Ala Glu Leu Asp Val1 300 Ala Ser Gly Ala Thr 380 Ala Gly Val1 285 Thr Ile Ala Leu Lys 365 Ala Ala Gly 270 Tyr Leu Leu Val Ala 350 Pro Glu Ile PCT/iBOO/00923 Arg Gly Phe Ser Ala Thr Gly Leu 320 Ile Tyr 335 Glu Ala Giu Ala Asp Val Lys Val 400 <210> 911 <211> 2409 <212> DNA <213> Corynebacteriun glutamicum <22 0> <221> CDS <222> (101)..(2386) <223> RXN00537 <400> 911 acccggaaca tgccgtcgaa aagctaaccg gcccatctat tgtccgccgt tggcaccatc gcggcttaag aggagtaaat aat gac Asn Asp ttt gag Phe Glu atc ctt Ile Leu atg tgg gca atc aag Ala Ile Lys aaa gac gac Lys Asp Asp acc gac gcc Thr Asp Ala acc cct Thr Pro gag tac Glu Tyr gag ctg Glu Leu tcc tcc tgatggcctg gagctgttcc atg agc act ttt gtc Met Ser Thr Phe Val 1 gag ctg gat cag cca Glu Leu Asp Gin Pro gcg cgc atc aag gaa Ala Arg Ile Lys Giu acc gtt tac tcc gtc Thr Val Tyr Ser Val aag gtt cac ctg cgt 115 163 211 259 307 tcc aag 1250 WO 01/00843 Met Trp Ser Glu His Cys Ser Tyr Lys Ser Ser L 60 PCTIBOO/00923 tac Tyr ggc Gly gtg Val cac His gct Ala I gca Ala 150 ggc Gly gaa Glu I ctgI Leu tcc Ser gat As p 230 ggc Giy gag Giu I Val I tct 1027 ggt Gly ggc Gly ttC Phe ggc Giy 120 ggc Gly gac Asp tcc Ser gtc Val1 gtg Val 200 acc Thr atc Ile gag Giu gta Val ggt Gly 280 c tg gaa Giu gag Giu cgc Arg 105 gct Ala gca Ala aac Asn cat His ttC Phe 185 ggt Giy Gly gg t Gly cgc Arg ctc Leu 265 att Ile gca ac c Thr aac Asn gtg Vai gcg Ala cgc Arg cca Pro tac Tyr 170 gac Asp acc Thr aac Asn ggc Giy aag Lys 250 atc Ile cag Gin gcc acc Thr 75 gc t Ala gag Glu acc Thr cca Pro gac Asp 155 ggc Gly gat Asp ctc Leu aag Lys gtg Val 235 c tc Leu gag Giu gac Asp gca act Thr ggt Gly tcc Ser ggc Gly atc Ile 140 acc Thr aac Asn tcc Ser aag Lys gtg Val 220 tcc Ser cca Pro tgc Cys ctc Leu ggC gag Glu gtg Val cac His gtc Val 125 gc t Ala cag Gin tgc Cys tac Tyr gtg Val 205 atc Ile gtc Val gct Ala tgc Cys ggt Gly 285 gac gaa Giu gtc Val aac Asn 110 ggc Giy gtg Vai cgt Arg ctc Leu gca Ala 190 gaa Giu ctg Leu ctg Leu gtt Vai c tc Leu 270 ggc Giy 99C gct Ala 80 atc Ile cca Pro atc Ile ga t Asp ttt Phe 160 ctg Leu aac Asn ctc Leu.

ggt Gly tcc Ser 240 gtt Val ctg Leu gga Gly atg t

S

g

G

t

S

9

V

c

G

1 c

P

c

P

c

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a ys Val His Leu cc aag att ctt er Lys Ile Leu ga gac ggc aac iy Asp Giy Asn 100 cc ttc gta gag er Phe Val Giu 115 tc cgc gac att 'al Arg Asp Ile 130 ag ctg cgt ttc in Leu Arg Phe ct ggc gtt gtt ro Giy Val Vai ca aac atc ggt ro Asn Ile Gly 180 ca ctg gtc aac ro Leu Vai Asn 195 ag ctt gca ttc vs Leu Ala Phe Arg gcc Ala gcc Ala cca Pro atg Met ggt Gly gac Asp 165 ggc Giy gca Ala gca Ala ctt Leu gaa Glu 245 gca Aia gtc Val acc Thr gac tcc Ser 225 gca Ala 9gc Gly tac Tyr ct t Leu cgc 210 cgc Arg tcc Ser ga t Asp aag Lys gcg Al a 290 gtc acc Thr ttc Phe cc t Pro gct Ala 275 tgt Cys aac WO 01/00943 Ser Glu Leu 295 aac gtc cca 1075 Asn Val Pro 310 tcc gag tcc 1123 Ser Glu Ser gag cgt ttc 1171 Giu Arg Phe atc ggc gaa 1219 Ile Gly Giu 360 ggt gaa gtt 1267 Gly Giu Val 375 gtc tac aac 1315 Val Tyr Asn 390 ctc gaa ggc 1363 Leu Glu Gly gct tgg ctg 1411 Ala Trp Leu atc acc gag 1459 Ile Thr Glu 440 aag aac gcc 1507 Lys Asn Ala 455 ggc gtt gcg 1555 Gly Val Ala 470 cca aac act 1603 Pro Asn Thr Ala ctg Leu cag Gin ctc Leu 345 gtt Val1 gt t Val1 cgc Arg gag Giu aag Lys 425 cag Gin aat Asn atc Ile Gly Ala cgc Arg gag Giu 330 gag Glu acc Thr atc Ile cca Pro atc Ile 410 ctt Leu tac Tyr gct Ala tcc Ser gcg Al a Ala gca Ala 315 cgc Arg atc Ile gac Asp gac Asp gt t Val1 395 gc t Al a gtc Val1 gac Asp ggc Gly gcc Ala 475 cag Gin Gly 300 gag Glu atg Met tgt Cys gag Glu gca Ala 380 gct Ala cgc Arg gct Ala cgc Arg gtC Val 460 gac Asp ctt Leu Asp aac Asn tgt Cys gca Ala aag Lys 365 cct Pro cgc Arg cca Pro tca Ser tac Tyr 445 ttg Leu gca Ala gca Ala Gly a tg Met gct Ala aag Lys 350 gac Asp cca Pro cc t Pro gtc Val cca Pro 430 gt c Val1 cgt Arg tcc Ser ctg Leu Gly tct Ser gtt Val 335 tgg Trp cgc Arg tca Ser gag Glu gac Asp 415 gca Ala cgc Arg atc Ile ggc Gly gct Ala Met gca Ala 320 gtc Val1 gat Asp tac Tyr acc Thr aac Asn 400 gtt Val ctt Leu ggc Gly gac Asp cgt Arg 480 gag Glu Arg 305 gct Ala acc Thr gtc Val gtt Val atc Ile 385 cag Gin gaa Giu gca Ala aac Asn gaa Glu 465 tac Tyr gct Ala Val gaa Giu cc t Pro acc Thr gtg Val1 370 gat Asp gac Asp gag Glu tcc Ser acc Thr 450 gag Glu ac c Thr tac Tyr PCTIIBOO/00923 Asn Leu Asp atc ctg gct Ile Leu Ala 325 gaa aac gtt Glu Asn Val 340 tgc gca gaa Cys Ala Glu 355 gtc cac aac Val His Asn gaa ggc cct Glu Gly Pro gaa ctg cag Giu Leu Gin 405 atc aag gct Ile Lys Ala 420 cgc gcg ttt Arg Ala Phe 435 gtt cag gca Val Gin Ala acc aac cgt Thr Asn Arg aag ctc gag Lys Leu Glu 485 cgc aac gtg Arg Asn Val 1252 WO 01/00843 WO 0100843PCTIIBOO/00923 gtc cc 1651 Val Ser ggt cc 1699 Gly Ser cac ggt 1747 His Gly 535 ggt aac 1795 Gly Asn 550 acc cca 1843 Thr Pro atc ggc 1891 Ile Gly gag acc 1939 Giu Thr gct ggc 1987 Ala Gly 615 cgt ctt 2035 Arg Leu 630 gat ctg 2083 Asp Leu cac cag 2131 His Gin ctg ttC 2179 Leu Phe acc ggt Thr Gly 505 Cca gaa Pro Giu 490 gca Ala cgc cca gtg gct Arg Pro Val Ala 510 495 500 gtc acc aac tgc ctg aac ttc Val Thr Asn Cys Leu Asn Phe 515 aac gct ggt gtt Asn Ala Gly Val 525 gac gga tcc aag Asp Giy Ser Lys atg Met tgg cag tcc Trp Gin Phe gca Ala ctt ttg ggc att Leu Leu Gly Ile 545 aag gaa gca gtc Lys Giu Ala Val 530 cca gtg tcc ggc Pro Val Ser Gly ccc atc ctg cca Pro Ile Leu Pro 565 540 gtc tcc tC tac Val Ser Phe Tyr 555 gtc gtg ggt gtt Val Val Gly Val 570 aac cag act Asn Gin Thr ttg gga gtc Leu Gly Val ggt gac Gly Asp 560 gag Glu aac gtc Asn Val 585 ccc gat Phe Asp 600 ctc aac Leu Asn ctc cca ccc gag gac Leu Pro Ser Giu Asp 590 gag ttc ggt ggc cc Glu Phe Gly Gly Ser 605 ggt ctg cca cca gta Gly Leu Pro Pro Val ttg gac aac gtc gag cag agc Leu Asp Asn Val Glu Gin Ser 575 580 aac gat ctc tac ccc ctg ggc Asn Asp Leu Tyr Leu Leu Gly 595 acc tgg cag cag gtt tc ggc Ile Trp Gin Gin Val Ser Gly 610 gtt gac ccg ccc aac gag cag Val Asp Leu Leu Asn Giu Gin gca gac ctg tcc Ala Asp Leu Phe 6 2 0 625 tct gag ggc ggc Ser Giu Gly Gly 650 aaa aag gga atg Lys Lys Giy Met 665 acc tca ctg ttt Thr Ser Leu Phe 680 gtc ggt tc gat ctg Val Giy Ser Asp Leu 640 ctt ggc cag acc cc Leu Gly Gin Thr Leu 655 gat gct gat ccc cc Asp Val Asp Leu Ser ttc gct gca ccc cac Phe Ala Ala Ser His 645 gca gag cct gcg atc Ala Giu Leu Ala Ile 660 670 gct gag tcc Ala Giu Ser 685 gcc tcc Ala Ser cag acc cac cca tcc Gin Ile His Pro Ser 675 cgc acc gtg gtc gca Arg Ile Val Val Ala 690 1253 WO 01/00843 WO 0100843PCT1JIB00I00923 acc aac cgc ggc gaa gag ttg gaa aag cgc gca gca gag ctg ggt gtt 2227 Thr Asn Arg Gly Glu Glu Leu Glu Lys Arg Ala Ala Giu Leu Gly Val 695 700 705 cca gtg ttc aag ctg ggc tgc acc aac gat tca gcc gtc atc gct gtc 2275 Pro Val Phe Lys Leu Gly Cys Thr Asn Asp Ser Ala Val Ile Ala Val 710 715 720 725 aag ggc gca gac gtt gag ttc act gtt tcc gtg gag gaa ctc cgc gaa 2323 Lys Gly Ala Asp Val Glu Phe Thr Val Ser Val Glu Giu Leu Arg Glu 730 735 740 gca tgg acc aac act ttg cct gag gcc ttc ggt cac gca gtt gga gct 2371 Ala Trp Thr Asn Thr Leu Pro Giu Ala Phe Gly His Ala Val Gly Ala 745 750 755 aac gca gta gtt gca taattttctg ctgtgaagcc ggg 2409 Asn Ala Val Val Ala 760 <210> 912 <211> 762 <212> PRT <213> Corynebacteriun glutanicun <400> 912 Met Ser Thr Phe Val Asn Asp Thr Val Giu Asp Ala Ile Lys Thr Pro 1 5 10 Glu Leu Asp Gln Pro Phe Glu Ala Leu Gly Leu Lys Asp Asp Giu Tyr 25 Ala Arg Ile Lys Glu Ile Leu Gly Arg Arg Pro Thr Asp Ala Giu Leu 40 Thr Val Tyr Ser Val Met Trp Ser Giu His Cys Ser Tyr Lys Ser Ser 55 Lys Val His Leu Arg Tyr Phe Gly Glu Thr Thr Thr Glu Glu Met Ala 70 75 Ser Lys Ile Leu Ala Gly Ile Gly Giu Asn Ala Gly Val Val Asp Ile 90 Gly Asp Gly Asn Ala Val Thr Phe Arg Val Glu Ser His Asn His Pro 100 105 110 Ser Phe Val Giu Pro His Gin Gly Ala Ala Thr Gly Val Gly Gly Ile 115 120 125 Val Arg Asp Ile Met Ala Met Gly Ala Arg Pro Ile Ala Val Met Asp 130 135 140 Gin Leu Arg Phe Gly Ala Leu Asp Asn Pro Asp Thr Gin Arg Vai Phe 1254 WO 01/00843 145 Pro Gly Va Pro Asn Il Pro Leu Va 19 Lys-Leu Al 210 Ser Arg Tb 225 Ala Ser Ph Gly Asp Pr Tyr Lys Al 27 Leu Ala Cy 290 Arg Val As 305 Ala Giu Il Thr Pro Gl Val Thr Cy Val Val Va 370 Ile Asp Gl 385 Gin Asp GI Glu Glu Ii Ala Ser Ax 43 Asn Thr Va 450 PCTIIBOOOO923 Val Asp Gly Ile Ser Hius Gly 180 Asn Phe Gly Glu Phe 260 Gly Ala Leu Leu Asn 340 Ala His Gly Leu Lys 420 Ala Gin 165 Gly Ala Ala Leu Giu 245 Ala Val Thr Asp Ala 325 Val Giu Asn Pro Gin 405 Ala Phe Ala Thr Cys Giy 215 Giy Giu Lys Val1 Glu 295 Val Giu Arg Gly Glu 375 Tyr Giu Trp, Thr Asn 455 Val Val 200 Thr Ile Giu Val Gly 280 Leu Pro Ser Phe Glu 360 Val Asn Gly Leu Glu 440 Ala Phe 185 Gly Gly Gly Arg Leu 265 Ile Ala Leu Gin Leu 345 Val Val1 Arg Glu Lys 425 Gin Asn Tyr 170 Asp Thr Asn Gly Lys 250 Ile Gin Ala Arg Glu 330 Glu Thr Ile Pro Ile 410 Leu Tyr Ala Ser Gly Asn Cys Leu Gly Leu Asp Leu Lys Val 235 Leu Giu Asp Ala Ala 315 Arg Ile Asp Asp Val 395 Ala Val Asp Gly Ala 475 Ser Lys Val 220 Ser Pro Cys Leu Gly 300 Giu Met Cys Giu Ala 380 Ala Arg Ala Arg Val 460 Asp Tyr Val 205 Ile Val Ala Cys Gly 285 Asp Asn Cys Ala Lys 365 Pro Arg Pro Ser Tyr 445 Leu Al a Ala 190 Glu Leu Leu Val1 Leu 270 Gly Gly Met Ala Lys 350 Asp Pro Pro Val Pro 430 Val Arg Ser 175 Gly Asp Phe Gly Gin 255 Giu Gly Gly Ser Val 335 Trp Arg Ser Giu Asp 415 Ala Arg Ile Gly Asn Leu Gly Ser 240 Val1 Leu Gly Met Ala 320 Val Asp Tyr Thr Asn 400 Val Leu Gly Asp Arg 480 Giu Giu Thr Asn Arg Gly Val Ala Ile 465 470 1255 WO 01/00843 WO 010084PCT/IBOO/00923 Tyr Thr Lys Leu Giu Pro Asn Thr Gly Ala Gin Leu Ala Leu Ala Giu 485 490 495 Ala Tyr Arg Asn Val Val Ser Thr Gly Ala Arg Pro Val Ala Val Thr 500 505 510 Asn Cys Leu Asn Phe Gly Ser Pro Giu Asn Ala Gly Val Met Trp Gin 515 520 525 Phe Lys Giu Ala Val His Gly Leu Ala Asp Gly Ser Lys Leu Leu Gly 530 535 540 Ile Pro Val Ser Giy Gly Asn Val Ser Phe Tyr Asn Gin Thr Gly Asp 545 550 555 560 Glu Pro Ile Leu Pro Thr Pro Val Val Gly Val Leu Gly Val Leu Asp 565 570 575 Asn Val Giu Gin Ser Ile Gly Asn Vai Leu Pro Ser Glu Asp Asn Asp 580 585 590 Leu Tyr Leu Leu Gly Giu Thr Phe Asp Glu Phe Giy Gly Ser Ile Trp 595 600 605 Gin Gin Val Ser Gly Ala Gly Leu Asn Gly Leu Pro Pro Val Val Asp 610 615 620 Leu Leu Asn Giu Gin Arg Leu Ala Asp Leu Phe Val Gly Ser Asp Leu 625 630 635 640 Phe Ala Ala Ser His Asp Leu Ser Giu Gly Gly Leu Gly Gin Thr Leu 645 650 655 Ala Giu Leu Ala Ile His Gin Lys Lys Gly Met Asp Vai Asp Leu Ser 660 665 670 Gin Ile His Pro Ser Leu Phe Thr Ser Leu Phe Ala Giu Ser Ala Ser 675 680 685 Arg Ilie Val Val Ala Thr Asn Arg Gly Glu Giu Leu Giu Lys Arg Ala 690 695 700 Ala Giu Leu Gly Val Pro Val Phe Lys Leu Gly Cys Thr Asn Asp Ser 705 710 715 720 Ala Val Ile Ala Val Lys Gly Ala Asp Val Giu Phe Thr Val Ser Val 725 730 735 Giu Giu Leu Arg Giu Ala Trp Thr Asn Thr Leu Pro Giu Ala Phe Gly 740 745 750 His Ala Val Gly Ala Asn Ala Val Val Ala 755 760 <210> 913 <211> 638 <212> DNA <213> Corynebacteriun glutamicun 1256 WO 01/00843 WO 0100843PCTJ/iBOO/00923 <220> <221> CDS <222> (54)..(638) <223> FRXA02805 <400> 913 tgtgatggat cagctgcgtt tcgggtgcac tggacaaccc agacacccag cgtgtg ttt 59 Val Phe 1 cct ggc gtt Pro Gly Val gtt gac ggc att Val Asp Gly Ile cat tac ggc aac His Tyr Gly Asn ctc ggc ctg Leu Gly Leu cca aac Pro Asn atc ggt ggc gaa Ile Gly Gly Glu gtc ttc gac gat Val Phe Asp Asp tac gca ggt aac Tyr Ala Gly Asn ctg gtc aac gca Leu Val Asn Ala tgc gtg ggt acc Cys Val Gly Thr aag gtg gaa gac Lys Val Glu Asp aag ctt gca ttc Lys Leu Ala Phe tcc ggc acc ggc Ser Gly Thr Gly aac Asn aag gtg atc ctg Lys Val Ile Leu ttC ggt Phe Gly tcc cgc acc Ser Arg Thr gca tcc ttc Ala Ser Phe ggC Gly ctt gat ggc atc Leu Asp Gly Ilie ggc gtg tcc gtc Gly Val Ser Val ctg ggt tcc Leu Gly Ser gtt cag gtt Val Gin Val gaa gaa ggc gaa Giu Glu Gly Glu cgc aag ctccca Arg Lys Leu Pro ggc gat Gly Asp 100 cct ttc gca gag Pro Phe Ala Glu gta ctc atc gag Val Leu Ile Glu tgc Cys 110 tgc ctc gag ctg Cys Leu Glu Leu aag gct ggc gtc Lys Ala Gly Val gtc ggt att cag Val Gly Ile Gin gac Asp 125 ctc ggt ggc ggc Leu Gly Gly Gly ctt gcg tgt gca Leu Ala Cys Ala tct gag ctg gca Ser Glu Leu Ala gca ggc gac ggc Ala Gly Asp Gly ggc atg Gly Met 145 cgc gtc aac Arg Val Asn gct gaa atc Ala Glu Ile 165 c ta Leu 150 gac aac gtc cca Asp Asn Val Pro cgc gca gag aac Arg Ala Glu Asn atg tct gca Met Ser Ala 160 gct gtt gtC Ala Val Val ctg gct tcc gag Leu Ala Ser Glu cag gag cgc atg Gin Glu Arg Met acc cct Thr Pro 180 gaa aac gtt gag Glu Asn Val Glu ttc ctc gag atc Phe Leu Giu Ile tgt Cys 190 gca aag tgg gat Ala Lys Trp, Asp 1257 WO 01/00843 WO 0100843PCT/EBOO/00923 <210> 914 <211> 195 <212> PRT <213> Corynebacterium giutamicun <400> 914 Val Phe Pro Gly Val Val Asp Giy Ile 1 Giy Gly Asp Phe Gly Gin Glu Gly Giy 145 Ser Val Trp Pro Pro Lys Ser Aia Giy Tyr 115 Leu Arg Ala Thr Val 195 5 Ilie Val Aila Thr Phe Pro Ala Cys Asn Ile 165 Glu Giy Asn Phe Gly Glu Phe Giy Ala Leu 150 Leu Asn Ser 10 Val Val Thr Ile Giu 90 Val Gly Leu Pro Ser 170 Phe Gly Asp Leu Lys Val Leu Glu Asp 125 Ala Ala Arg Ile Asn Ser Lys Val1 Ser Pro Cys 110 Leu Gly Glu Met Cys 190 Cys Tyr Val Ile Val Ala Cys Gly Asp Asn Cys 175 Ala <210> 915 <211> 697 <212> DNA <213> Corynebacteriun glutanicun <2 <221> CDS <222> (23)..(697) <223> FRXA00537 <400> 915 caacagcact tgcatcccgc gcgtg tat cac cga gca gta ctg aac gct acg 52 Val Tyr His Arg Ala Val Leu Asn Ala Thr 1258 WO 01/00843 WO 0100843PCT/IBOO/00923 tcc gcg gca aca Ser Ala Ala Thr ttc aag caa aag Phe Lys Gin Lys aac gcc aat gct ggc gtc ttg Asn Ala Asn Ala Gly Val Leu cgt atc gac Arg Ile Asp tcC ggc cgt Ser Gly Arg gag acc anc cgt Giu Thr Xaa Arg gtt gcg atc tcc Val Ala Ile Ser gcc gac gca Ala Asp Ala cag ctt gca Gin Leu Ala tac acc aag ctc Tyr Thr Lys Leu cca aac act ggc Pro Asri Thr Gly ctg gct gag gct tac cgc aac gtg gtc tcc acc ggt gca cgc cca gtg Leu Ala Giu Ala Tyr Arg Asn Val Val Ser Thr Gly Ala Arg Pro Val 65 gct Ala gtc acc aac tgc Val Thr Asn Cys aac ttc ggt tcc Asn Phe Gly Ser cca gaa aac gct Pro Giu Asn Ala ctg gca gac gga Leu Ala Asp Gly ggt Gly atg tgg cag ttc Met Trp Gin Phe gaa gca gtc cac GlU Ala Val His ggt Gly 100 tcc aag Ser Lys 105 ctt ttg ggc Leu Leu Gly act ggt gac Thr Gly Asp 125 att Ile 110 cca gtg tcc ggc Pro Val Ser Gly ggt Gly 115 aac gtc tcc ttc Asn Val Ser Phe tac aac cag Tyr Asn Gin 120 gtt ttg gga Val Leu Gly gag ccc atc ctg Giu Pro Ile Leu acc cca gtc gtg- Thr Pro Val Val ggt Gly 135 gtc ttg Val Leu 140 gac aac gtc gag cag agc atc ggc aac Asp Asn Val Giu Gin Ser Ile Gly Asn 145 ctc cca tcc gag Leu Pro Ser Giu aac gat ctc tac Asn Asp Leu Tyr c tc Leu 160 ctg ggt gag acc Leu Gly Giu Thr gat gag ttc ggt Asp Giu Phe Gly ggc Gly 170 tcc atc tgg cag Ser Ile Trp Gin cag Gin 175 gtt tct ggc gct Val Ser Gly Ala ctc aac ggt ctg Leu Asn Gly Leu cca cca Pro Pro 185 gta gtt gac Val Val Asp tct gat ctg Ser Asp Leu 205 ctc aac gag cag Leu Asn Glu Gin ctt gca gac ctg Leu Ala Asp Leu ttc gtc ggt Phe Val Gly 200 ttt gct gca tcc Phe Ala Ala Ser cac gat ctg tct gag ggc ggc ctt ggc His Asp Leu Ser Giu Gly Gly Leu Gly 210 215 cag acc ctc gca gag ctt Gin Thr Leu Ala Giu Leu gcg 697 Ala 225 <210> 916 <211> 225 1259 WO 01/00843 <212> PRT <213> Corynebacterium giutamicum <400> 916 Val Tyr His Arg Ala Vai Leu Asn Ala PCTIiBOO/00923 1.

Lys Xaa Leu Asn Asn Ala Ser Leu Gin 145 Leu Ser Giu Gin Arg Giu Val Phe Vai Gly Pro 130 Ser Gly Gly Gin Lys Giy Pro Val Gly His Gly 115 Thr Ilie Giu Ala Arg 195 Asn Vali Asn Ser Ser Gly 100 Asn Pro Gly Thr Gly 180 Leu Giy Aia Gin Arg Aia Gly Tyr 120 Val1 Pro Phe Leu Phe 200 Thr Leu Ala Ala Val1 Val1 Lys Gin Gly Glu Gly 170 Pro Gly Ser Arg Ser Leu Ala 75 Met Leu Thr Val1 Asp 155 Ser Val1 Ser Thr Giu Tyr Ala Asn Phe Ile 110 Giu Asn Leu Gin Leu 190 Phe Phe Thr Lys Arg Leu Giu Val1 Ile Giu Leu 160 Val1 Asn Ala Ser His Asp 210 Leu Ser Giu Gly Gly Leu Giy Gin Thr Leu Ala Giu Leu Ala 225 <210> 917 <211> 302 <212> DNA <213> Corynebacterium giutamicum <22 0> <221> CDS <222> .(279) <223> FRXA00561 <400> 917 1260 WO 01/00843 ctc ttc cca gat cca ccc atc cct Leu Phe Pro Asp Pro Pro Ie Pro I 1 5 ccc gct tcc cgc atc gtg gtt gca Pro Ala Ser Arg Ile Val Val Ala aag cgc gca gca, gag ctg ggt gtt Lys Arg Ala Ala Glu Leu Gly Val aac gat tca gcc gtc atc gct gtc Asn Asp Ser Ala Val Ile Ala Val 1 55 gtt tcc gtg gag gaa ctc cgc gaa Val Ser Val Glu Glu Leu Arg Glu 2 70 gcc ttc ggt cac gca gtt gga gct Ala Phe Gly His Ala Val Gly Ala 2 ctgtgaagcc ggg <210> 918 <211> 93 <212> PRT <213> Corynebacterium giutainicum <400> 918 Leu Phe Pro Asp Pro Pro Ile ProI 1 5 Pro Ala Ser Arg Ile Val Val Ala Lys Arg Ala Ala Glu Leu Gly Val 40 Asn Asp Ser Ala Val Ile Ala Val 1 55 Val Ser Val Glu Glu Leu Arg Glu 2 70 Ala Phe Gly His Ala Val Gly Ala 2 <210> 919 <211> 792 <212> DNA <213> Corynebacterium glutamicum <2 <221> CDS <222> (101). .(769) <223> RXA00541 act Thr ggC Gly aag Lys gac

ASP

aac Asn gtt Val PCTJIBOO/00923 ttg ctg agt 48 Leu Leu Ser gag ttg gaa 96 Glu Leu Glu ggc tgc acc 144 Gly Cys Thr gag ttc act 192 Glu Phe Thr ttg cct gag 240 Leu Pro Glu taattttctg 289 Thr Gly Lys Asp Asn Val Cys Leu Leu Ser Glu Glu Leu Glu Leu Gly Cys Thr Val Glu Phe Thr Thr Leu Pro Giu Ala 1261 WO 01/00843 WO 0100843PCTIIBOO/00923 <400> 919 atgattccgt caccgaagct gacctaaaga aaattgctga tcatcgaaga cttcgatgtg gtgggagttg aggtcgcgaa aaccctcctc gcaaacaccg gtg agc gcc aaa Val Ser Ala Lys 1 ggt gtc att acc Gly Val Ile Thr cca ggc acc ctt Pro Gly Thr Leu gat gta gat gca Asp Val Asp Ala gca cgc Ala Arg gct gct cgc Ala Ala Arg gag gat ctc Giu Asp Leu atc Ile gca ggt gca gaa Ala Gly Ala Glu atc agc ctg tgg Ile Ser Leu Trp cac gct gac His Ala Asp gga ttc tcc Gly Phe Ser aag ggc gtc gac Lys Gly Val Asp gtt gtc gtt ccc Val Val Val Pro gg t Gly tac ggc Tyr Gly gat tac ctg cgc Asp Tyr Leu Arg ggt gca atc tct Gly Ala Ile Ser ctg gcg cca gta Leu Ala Pro Val cag tcc gtg att Gin Ser Val Ile cag gcc ggt aag Gin Ala Gly Lys atg cca gtc ttg Met Pro Val Leu att tgc aac ggc Ile Cys Asn Gly cag atc ctc acc Gin Ile Leu Thr gca cgc ctg ctt Ala Arg Leu Leu cca ggc Pro Gly 100 gcg ctg acc Ala Leu Thr ctc gtt gta Leu Val Val 120 cgc Arg 105 aac aag ggt ctg Asn Lys Giy Leu ttt cac tgt gta Phe His Cys Val gac gca cac Asp Ala His 115 ttg gaa aag Leu Glu Lys gag aac aac acc Giu Asn Asn Thr gca tgg acc aac Ala Trp Thr Asn act Thr 130 ggt cag Gly Gin 135 cag atc ctt att Gin Ile Leu Ile gca aag cac ggt Ala Lys His Gly ggt cgc ttc cag Gly Arg Phe Gin gac gca gag acc Asp Ala Glu Thr atc Ile 155 gcc cag ctt gag Ala Gin Leu Giu gaa ggc cgc gtg Giu Gly Arg Val ttc cgt tac acc Phe Arg Tyr Thr ga t Asp 170 aac ttc aac ggt Asn Phe Asn Gly gtc aac gat atc Val Asn Asp Ile gcc ggt Ala Gly 180 atc act aat Ile Thr Asn cat gcc gtc His Ala Val 200 act ggt cgc atc Thr Gly Arg Ile ggt ctc atg ccg Gly Leu Met Pro cac ccg gaa His Pro Giu 195 ctg gag ctg Leu Glu Leu gaa aag cta acc Giu Lys Leu Thr cca tct att gat Pro Ser Ile Asp ttc cig tcc gcc gtt ggc acc atc gcg gct taagaggagt aaatatgagc 1262 WO 01/00843 Phe Leu Ser Ala Val Gly Thr Ile Ala Ala 215 220 act <210> 920 <211> 223 <212> PRT <213> Corynebacterium glutamicum PCT/1IBOO/00923 <400> 920 Val Ser Ala Lys Ilie Gly Val Ile Thr 1 Val Leu Pro Al a Met Arg Cys Asn Glu 145 Glu Asn Met Asp Trp Gly Leu Pro Leu Val Thr 130 Gly Gly Asp Pro Ala His Gly Ala Val1 Leu Asp 115 Leu Arg Arg Ile His 195 Phe Ala Gly Leu Ile Phe Asn Asn Leu Thr Asp 170 Thr Lys Pro Gly Val1 Arg Glu Gin Lys Asn Ile Ile 155 Asn Gly Leu Thr Glu Ala Gly Ala Leu Leu Thr 125 Ala Gin Asn Ile Gly 205 Asp Gly Leu Glu Leu Phe Leu Ser Ala Val Gly Thr Ile Ala Ala 210 215 220 <210> 921 <211> 1014 <212> DNA <213> Corynebacteriun glutamicum <22 0> 1263 WO 01/00843 WO 0100843PCT/EBO0100923 <221> CDs <222> (101)..(991) <223> RXA00620 <400> 921 ggtgatccat gtcaggaagc cagcggtgaa accggcagtg aattttccga acacacccga ggggtctaga cttgcctaac aaaccagcgg tgaatgctaa atg cgt cct gaa Met Arg Pro Glu ctc Leu tcc cag tac aag cac ctg tcg gca ggc Ser Gin Tyr Lys His Leu Ser Ala Gly gtc cgt gag atc Val Arg Giu Ile tac gag Tyr Giu atc gac gac Ile Asp Asp tac gat ttc Tyr Asp Phe aag Lys cac atc ctc atg His Ile Leu Met gct tcc gat cgt Ala Ser Asp Arg atc tct gca Ile Ser Ala cga gtg ctc Arg Val Leu atc ctc gat acc Ile Leu Asp Thr att cca gac aag Ile Pro Asp Lys act gcg Thr Ala atg agc cag ttc Met Ser Gin Phe ttc gac acc atc Phe Asp Thr Ile ttt cct aat cac Phe Pro Asn His gca ggt ccc gct Ala Giy Pro Ala gat cca cgt atc Asp Pro Arg Ile cca Pro gaa gaa gtt ttg Glu Giu Val Leu cga gca atg gtg Arg Ala Met Vai aag aag ctc aac Lys Lys Leu Asn ctt cct ttt gaa Leu Pro Phe Giu tgc gtg Cys Vai 100 gtt cgt gga Val Arg Giy tac Tyr 105 ctc act ggc tct Leu Thr Gly Ser ctt gtt gaa tac aag cag acc Leu Val Giu Tyr Lys Gin Thr 115 agc tcc gtg tgt gga gtt gag ctc cca gaa ggc ctc gtt Ser Ser Val Cys Gly Val Glu Leu Pro Glu Gly Leu Vai 120 125 130 gaa tct tct Giu Ser Ser cag ctg Gin Leu 135 cct gag cca atc Pro Giu Pro Ile acc cca gcc acc Thr Pro Ala Thr aag Lys 145 gct gac atc ggc Ala Asp Ile Gly cac gac atc aat His Asp Ile Asn tcc ttt gac gtt Ser Phe Asp Val gaa gaa cgt ctc Giu Glu Arg Leu gaa gct cgt gcg Giu Aia Arg Ala aac Asn 170 cag ttg cgc gat Gin Leu Arg Asp tct att gct att Ser Ile Ala Ile tac aag Tyr Lys 180 gct gct gct Ala Aia Ala gag Glu i185 atc gcc cgt gac Ile Ala Arg Asp ggc gtc atc ctt Gly Vai Ile Leu gcc gac acc Ala Asp Thr 195 aaa ttt gag ttc ggc atc gat gaa gat ggc acc ctc gtg ctt ggt gat Lys Phe Glu Phe Gly Ile Asp Giu Asp Giy Thr Leu Vai Leu Giy Asp 1264 WO 01/00843 WO 0100843PCTIIBOO/00923 200 205 gaa gtc ctt acc cca gat tcc tcc cgc tac tgg Giu Vai Leu Thr Pro Asp Ser Ser Arg Tyr Trp 215 220 gaa gca gga tct gtg caa cca agc ttt gat aag Giu Ala Gly Ser Vai Gin Pro Ser Phe Asp Lys 230 235 240 tgg ctc acc ggc cct aaa tct ggc tgg gac aag Trp Leu Thr Gly Pro Lys Ser Gly Trp Asp Lys 250 255 cca cca gct ctg cca ggt tcc gtt gtt gag gca Pro Pro Ala Leu Pro Gly Ser Val Vai Giu Ala 265 270 atc gag gcc tac gag ctg att tct ggt cag aag Ile Giu Ala Tyr Glu Leu Ile Ser Gly Gin Lys 280 285 ggt tct tgc gtc taagctgctt gattttccct aaa 1014 Gly Ser Cys Val 295 <210> 922 <211> 297 <212> PRT <213> Corynebacterium giutamicum <400> 922 Met Arg Pro Glu Leu Ser Gin Tyr Lys His Leu 1 5 10 Arg Giu Ile Tyr Glu Ile Asp Asp Lys His Ile 25 Asp Arg Ile Ser Ala Tyr Asp Phe Ile Leu Asp 40 Lys Gly Arg Val Leu Thr Ala Met Ser Gin Phe 55 Asp Phe Pro Asn His Leu Ala Gly Pro Ala Asp 70 Giu Giu Val Leu Gly Arg Ala Met Val Cys Lys 90 Pro. Phe Giu Cys Val Val Arg Gly Tyr Leu Thr 100 105 Giu Tyr Lys Gin Thr Ser Ser Val Cys Gly Val 115 120 Leu Val Glu Ser Ser Gin Leu Pro Glu Pro Ile 130 135 cct Pro 225 caa Gin gat Asp acc Thr ttc Phe Ser Leu Thr Phe Asp Lys Gly Glu Phe 140 210 ttg Leu t tc Phe tcc Ser cgc Arg tgc Cys 290 Al a Met Giu Phe Pro Leu Ser Leu 125 Thr gaa Giu gtg Val ggc Gly gag Giu 275 cag Gin Gly Val Ile Asp Arg Asn Gly 110 Pro Pro 9gC Gly cgc Arg ttg Leu 260 cgc Arg tgg Trp Lys Ala Pro Thr Ile Met Leu Giu Ala tat Tyr aac Asn 245 gag Giu tac Tyr att Ile 1265 WO 01/00843 Lys Ala Asp Ile Gly Asp His Asp Ile Asn Val 145 '150 155 Giu Glu Arg Leu Gly Giu Ala Arg Ala Asn Gin 165 170 Ilie Ala Ile Tyr Lys Ala Ala Ala Glu Ile Ala 180 185 Ilie Leu Ala Asp Thr Lys Phe Giu Phe Gly Ilie 195 200 Leu Val Leu Gly Asp Giu Val Leu Thr Pro Asp 210 215 Pro Leu Giu Giy Tyr Glu Ala Gly Ser Vai Gin 225 230 235 Gin Phe Vai Arg Asn Trp, Leu Thr Gly Pro Lys 245 250 Asp Ser Gly Leu Glu Pro Pro Ala Leu Pro Gly 260 265 Thr Arg Glu Arg Tyr Ile Giu Ala Tyr Giu Leu 275 280 Phe Cys Gin Trp Ilie Gly Ser Cys Val 290 295 <210> 923 <211> 1293 <212> DNA <213> Corynebacteriun giutanicun <22 0> <221> CDS <222> (101)..(1270) <223> RXN00770 <400> 923 ccatgggtct gccacaggga aacagcaacg cagacctagt cctcaagtta agatcggtag gcgatagggg ttgagcattt Asp Asp Arg 190 Asp Arg Phe Trp Val1 270 Gly PCTIIBOO/00923 Val Val 160 Ala Ser 175 Gly Val Gly Thr Tyr Trp Asp Lys 240 Asp Lys 255 Giu Ala Gin Lys gcg Ala ttC Phe gac Asp gaa tcc Ser gca Ala gaa Giu cgt cgc Arg gga Gly ggc Gly gcc t ta Leu aag Lys tac Tyr ctc 1266 ccgcaagatg caagcaaccg ttg ctc tcc ccg tat Leu Leu Ser Pro Tyr 1 tat gtt cct aat aag Tyr Val Pro Asn Lys atg agt gat cac cag Met Ser Asp His Gin gca gga gtc gac atc Ala Gly Val Asp Ile cca atg gcc aag cgc WO 01/00843 Glu Ala Gly Asp Arg Ala Val Glu Leu Phe Ala Pro Met Ala PCTIiBOO/00923 60 gcc Ala ttt Phe gga Gly acc Thr acc Thr gt t Val 150 tgt Cys cc a Pro ggc Gly ggc Gly tac Tyr 230 gac Asp gag Glu tgc Cys aac acc Thr gag Glu gtc Val1 atc Ile ggc Gly 135 gtc Val gtc Val ggt Gly gtt Val gac Asp 215 tc Ser gga Gly cca Pro gaa Glu ctc cgc Arg ctc Leu ggc Gly ggc Gly 120 gca Ala cca Pro cag Gin gtt Val1 gtc Val 200 gtc Val1 ctg Leu cac His acc Thr gtt Val 280 gag gtt Val 75 tac Tyr ctt Leu ctt Leu ctg Leu gtt Val 155 tgt Cys cca Pro gat Asp ggC Gly cac His 235 gaa Glu tac Tyr ttc Phe gtc ctt Leu aag Lys gtc Val gtt Val ttc Phe 140 gc t Ala gc t Ala gac Asp gaa Glu atg Met 220 gtc Val1 ctc Leu gcc Ala tgc Cys cca ggc Gly aag Lys atc Ile gca Ala 125 ctc Leu gag Giu ctg Leu cac His c tg Leu 205 gct Ala ctc Leu gga Gly aag Lys cac His 285 gaa aac Asn cca Pro gcc Ala 110 atg Met cag Gin atc Ile ctc Leu tac 190 cta Leu tcc Ser ctg I.eu cgc Arg gac Asp 270 gtc Val 999 ctc Leu atc Ile cag Gln tgt Cys gac Asp gtc Vai ggt Gly 175 gat Asp gga Gly tcc Ser gaa Giu acc Thr 255 tgc Cys acc Thr ctc gga Gly 80 ctc Leu atg Met gtg Val tac Tyr tcc Ser 160 ggc Gly gtc Val cca Pro ggt Gly aag Lys 240 C tc Leu c tg Leu 99C Giy gtc 9gc Gly gca Ala atg Met ga t Asp atc Ile 145 gg t Gly gaa Glu tcc Ser gac Asp ctg Leu 225 gca Ala gg t Gly gca Ala 9gC Gly gca ttc Phe gca Ala gac Asp gac Asp 130 gcc Ala atc Ile ac c Thr gca Ala cgc Arg 210 cac His 9gC Gly gaa Glu ctg Leu 9gC Gly 290 gaa gca Ala gga Gly aag Lys 115 ctc Leu atc Ile gca Ala gca Al a act Thr 195 gtc Val tcc Ser ctg Leu gaa Glu atc Ile 275 ctc Leu atg Lys gga Gly tct Ser 100 cac His gtt Val1 ggC Gly gaa Glu gaa Glu 180 gca Ala cgc Arg aac Asn gcg Ala ctt Leu 260 gca Ala gca Ala tcc Arg ctc Leu gac Asp gac Asp gtc Val aag Lys ggc Gly 165 cac His gtc Val gca Ala ggt Gly ctt Leu 245 ctc Leu gag Glu 9gc Gly cga 355 403 451 499 547 595 643 691 739 787 835 883 931 979 1027 1267 WO 01/00843 Asn Leu GI 295 gca act tq 1075 Ala Thr Ti 310 aag gtt tc 1123 Lys Val SE atg gtt gc 1171 Met Val Al ctc acc gc 1219 Leu Thr Al 36 ggt gaa ga 1267 Arg acc Thr cgc Arg gtc Val 345 cgt Arg gga Val1 cca Pro gaa Gi u 330 gtt Val cac His gag Val acc Thr 320 ttc Phe gac Asp* atc Ile aac Al a 305 atc Ile aac Asn cgc Arg gga Gly ggc Glu tcc Ser atg Met gcc Ala acc Thr 370 gag PCT/iBOOIOO923 Ser Arg gtg ggc Val Gly 325 gtc ggc Val Gly 340 gca atg Ala Met cgc aac Arg Asn cct ggc Gly Giu Glu Gly Giu Pro 375 Arg Val Ile Leu Asn Gly Glu His Pro Gly 380 385 tac taagcccaac tgtctgctct aag 1293 Tyr 390 <210> 924 <211> 390 <212> PRT <213> Corynebacteriun giutamicum <400> 924 Leu Leu Ser Pro Tyr Ala Trp Gly Leu 1 5 Tyr Val Pro Asn Lys Phe Gin Thr Pro 25 Met Ser Asp His Gin Asp Thr Thr Ala 40 Ala Gly Val Asp Ile Giu Ala Gly Asp Pro Met Ala Lys Arg Ala Thr Arg Pro Gly Phe Ala Gly Leu Phe Glu Leu Gly Ala Ala Gly Ser Asp Gly Val Gly Thr 100 105 Leu Ala Ser Giu Gly Lys Ile Leu Lys Tyr Leu Asn Pro Ala 110 Asp Tyr Ala Phe Leu Ile Gin 1268 WO 01/00843 WO 0100843PCTIIBOO/00923 Met Asp Lys His Asp Thr Ile Gly Ile Asp Leu Val Ala Met Cys Val 115 120 125 Asp Ile 145 Gly Glu Ser Asp Leu 225 Ala Gly Ala Gly Ala 305 Ile Asn Arg Gly Asp 130 Ala Ile Thr Ala Arg 210 His Gly Giu Leu Gly 290 Gi u Ser Met Ala Thr 370 Val1 Gly Giu Giu 180 Ala Arg Asn Ala Leu 260 Ala Ala Ser Val1 Val 340 Ala Arg Thr Val 150 Cys Pro Gly Gly Tyr 230 Asp Giu Cys Asn Ala 310 Lys Met Leu Giy Gly 135 Vai Val1 Gly Val1 Asp 215 Ser Gly Pro Giu Leu 295 Thr Vai Va 1 Thr Glu 375 Ala Pro Gin Val1 Val 200 Val Leu His Thr Vai 280 Giu Trp Ser Ala Ala 360 Giu Glu Ala met 185 Giu Leu Ala Ile Arg 265 His Arg Thr Arg Val 345 Arg Pro His Giy 170 Giu Ala Ile Arg Giu 250 Ile Thr Val Pro Glu 330 Val His Leu Val 155 Cys Pro Asp Giy His 235 Giu Tyr Phe Val Gly 315 Giu Ala Ile Phe 140 Aia Aia Asp Giu Met 220 Vai Leu Ala Cys Pro 300 Gin Met Giu Asp Leu Giu Leu His Leu 205 Ala Leu Gly Lys His 285 Giu Ile Giu Lys Cys 365 Gin Ile Leu Tyr 190 Leu Ser Leu Arg Asp 270 Vai Giy Phe Lys Asp 350 Trp Asp Vai Gly 175 Asp Gly Ser Giu Thr 255 Cys Thr Leu Arg Thr 335 Arg Giu Tyr Ser 160 Gly Val Pro Giy Lys 240 Leu Leu Gly Vai Thr 320 Phe Asp Ile Giu Gly Giu Pro Arg Val Ile Leu Asn Gly Giu His Pro Giy Tyr 385 390 <210> 925 <211> 818 <212> DNA <213> Corynebacterium giutamicun <220> 1269 WO 01/00843 <221> CDS <222> (15)..(818) <223> FRXA00557 <400> 925 tgggttgagc atttttg ctc tcc ccg tat gcg tgg ggg ttg tcc cgc Leu Leu Ser Pro Tyr Ala Trp, Gly Leu Ser Arc ctt tta gac agt tat gtt cct aat aag ttc caa acc cca gca Leu gcg Ala tca Ser gaa Giu Gly aag Lys atc Ile gca Ala 125 ctc Leu gag Giu ctg Leu cac His ctg Leu 205 Leu aag Lys tac Tyr ctc Leu aac Asn c ca Pro gcc Ala 110 a tg Met cag Gin atc Ilie C tC Leu t ac Tyr 190 c ta Leu

ASP

tac Tyr gca Ala tt t Phe ctc Leu atc Ile cag Gin tgt Cys gac Asp gtc Val1 ggt Gly 175 ga t Asp gga Gly Ser acg Thr gca Ala gca Ala gga Gly ctc Leu atg Met gtg Val tac Tyr tcc Ser 160 ggc Giy gtc Val cca Pro Tyr atg Met gca Ala cca Pro ggc Giy gca Ala atg Met gat Asp atc Ile 145 ggt Gly gaa Glu tcc Ser gac Asp Val agt Ser gga Gly 50 atg Met ttc Phe gca Ala gac Asp gac Asp 130 gcc Ala atc Ile acc Thr gca Ala cgc- Arg 210 Pro gat Asp 35 gtc Val gcc Ala gca Ala gga Gly aag Lys 115 ctc Leu atc Ile gca Ala gca Ala act Thr 195 gtc Val Lys cag Gin atc Ile cgc Arg c tc Leu gac Asp gac Asp gtc Val1 aag Lys ggc Gly 165 cac His gtc Val gca Ala Phe gac

ASP

gaa Glu gcc Al a 70 ttt Phe gga Gly acc Thr ac c Thr gtt Val 150 tgt Cys cca Pro ggc Gly ggc Gly Gin acc Thr gcc Aia 55 acc Thr gag Giu gtc Val1 atc Ile ggc Giy 135 gtc Val gtc Val ggt Gly gtt Val gac

ASP

215 Thr a cc Thr ggc Giy cgc Arg ctC Leu 9gc Gly ggc Gly 120 gca Aia cca Pro cag Gin gtt Val1 gtc Val 200 gtc Val1

A:

ci

LE

ci ci gi Vc 1! PCT/iBOO/00923 gca ;Ala ;a gaa 98 Ly Glu Ic gtt 146 Ly Vai gtc 194 la Val tt ctt 242 11 Leu ac aag 290 (r Lys tt gtc 338 au Val tt gtt 386 au Val tg ttc 434 ~u Phe 140 tt gct 482 11 Ala )t gct 530 's Ala gac 578 r-O Asp 5it gaa 626 ~p Giu ;c atg 674 ly met 220 1270 WO 01/00843 gct tcc tcc Ala Ser Ser ctc ctg gaa Leu Leu Glu gga cgc acc Gly Arg Thr 255 ggt ctg Gly Leu 225 aag gca Lys Ala 240 ccc ggt Pro Gly cac tcc His Ser ggc ctg Gly Leu PCTIBOOIOO923 tac tcc ctg gct cgc cac gtc 722 Tyr Ser Leu Ala Arg His Val 230 235 gac gga cac atc gaa gaa ctc 770 Asp Gly His Ile Giu Glu Leu 250 gag cca acc cgc atg tac gcc 818 Glu Pro Thr Arg Met Tyr Ala 265 gaa gaa ctt Glu Glu Leu 260 <210> 926 <211> 268 <212> PRT <213> Corynebacteriun glutainicum <400> 926 Leu Leu Ser Pro Tyr Ala Trp Gly Leu 1 Tyr Met Ala Pro Gly Ala Met Asp Ile 145 Giy Giu Ser Asp Phe Asp Giu Ala Phe Gly Thr Thr Val 150 Cys Pro Gly Gly Gin Thr Ala Thr Giu Val1 Ile Gly 135 Val1 Val Gly Val Asp 215 Ser 10 Ala Giu Arg Giu Lys Lys Asp Pro His Gly 170 Giu Ala Ile Ala Giu Val Val Leu Lys Val Val1 Phe 140 Ala Ala Asp Glu Leu Ala Ser Glu Gly Lys Ile Ala 125 Leu Giu Leu His Leu 205 Leu Lys Tyr Leu Asri Pro Ala 110 Met Gin Ile Leu Tyr 190 Leu Asp Tyr Aia Phe Leu Ile Gin Cys Asp Val Gly 175 Asp Gly Gly Met Ala Ser Ser Gly 220 1271 WO 01/00843 WO 0100843PCTIBOOOO923 Leu His Ser Asn Gly Tyr Ser Leu Ala Arg His 225 230 235 Ala Gly Leu Ala Leu Asp Gly His Ile Giu Giu 245 250 Val Leu Leu Giu Leu Gly Arg Thr Pro 255 Gly Giu Glu Leu Leu Giu Pro Thr Arg 260 265 <210> 927 <211> 338 <212> DNA <213> Corynebacteriun giutamicum Met Tyr Ala <220> <221> <222> <223>

CDS

FR.XA0 0770 <400> 927 gtc acc gi Val Thr G: 1 9c ggc ggC ly Gly Gly 5 ctc gca ggc aac Leu Ala Gly Asn gag cgg gtt gtc Giu Arg Val Val cca gaa Pro Glu ggg ctc gtc Gly Leu Val ttc cgc acc Phe Arg Thr gca Ala gaa atg tcc cga Glu Met Ser Arg act tgg acc cca Thr Trp Thr Pro ggc caa atc Gly Gin Ile atc tcc tct gtg Ile Ser Ser Val aag gtt tcc cgc gaa gaa atg gaa Lys Val Ser Arg Glu Giu Met Giu aag acc Lys Thr ttc aac atg ggt Phe Asn Met Gly ggc atg gtt gca Gly Met Val Ala gtt gct gaa aag Val Ala Giu Lys cgc gac cgc gcc Arg Asp Arg Ala ctg gca Leu'Ala atg ctc acc Met Leu Thr cgt cac att gac Arg His Ile Asp tgg gaa atc gga Trp Giu Ile Gly atc ctc aac ggc Ile Leu Asn Gly 100 gta cgc aac ggt Val Arg Asn Gly gag gga gag cct Glu Gly Glu Pro cgc gtg Arg Val gag cac cct ggc Giu His Pro Gly taagcccaac tgtctgctct <210> 928 <211> 105 <212> PRT <213> Corynebacterium giutamicun <400> 928 Val Thr Giy Gly Gly Leu Ala Giy Asn 1 5 Leu Giu Arg Val Val Pro Giu 10 1272 WO 01/00843 Gly Leu Va Phe Arg Tb 3 Lys Thr Ph Asp Arg As Trp Glu Ii Ile Leu As Ala Ile Asn Arg Gly Gly 100 Met Ser Ser Val Gly Val 55 Leu Ala 70 Val Arg His Pro Arg Gly 40 Gly Met Asn Gly Ala 25 Lys Met Leu Gly Tyr 105 Thr Trp Thr Pro Gly Val Ser Arg Giu Glu Val Ala Val Val Ala Thr Ala Arg His Ile 75 Glu Glu Gly Glu Pro PCTJIBOO/00923 Gin Ile Met Giu Giu Lys Asp Cys Arg Val <210> 929 <211> 1320 <212> DNA <213> Corynebacterium giutanicun <22 0> <221> CDS <222> (101)..(1297) <223> RXN02345 <400> 929 accatcgacc ctatgtaatc aaccaggttc ccaaggctcg aaaagtggaa gcgctgctca aagatcttac attttggtga aggcgttata gttaggactt gtg act tct aca gga Val Thr Ser Thr Gly aac caa Asn Gin ggc caa Gly Gin tca ctg Ser Leu gct gct Ala Ala gtc gcc Val Ala ccc aac Pro Asn cca cgc gcc Ala t ta Leu cga Arg gat Asp atc Ile gaa Giu cca cac His gcc Ala gt t Val1 gtt Val gaa Giu cac His gaa gtc Val gcc Ala t cc Ser aac Asn ttC Phe gaa Glu gac 1 gca Ala atc Ile t cc Ser att Ile gac Asp ggc Gly aaa att Ile C tc Leu gct Ala gat Asp gag Giu aac Asn gtC 1273 WO 01/00843 Pro Arg Pr aag cgt ct Lys Arg Le 12 gaa tca gt Giu Ser Va 135 gtt tgc ct Val Cys Le 150 ttc cca gc Phe Pro Al gac ggc gq Asp Gly GI gaa ctg tc Glu Leu Se 2C tgg cca gt Trp Pro Va 215 atc gct cc Ile Ala Pi 230 gga ttg gc Gly Leu Al gca gtg ga Ala Val GI ttt gtc aa Phe Val As caa gat gq 1027 Gin Asp GI 295 ctc gac ta 1075 Leu Asp T 310 atg gcc ae 1123 Met Ala As Leu ctc Leu gca Ala cgc Arg 155 gca Ala ctc Leu gtt Val tca Se r gaa Giu 235 atc Ile gaa Giu gcc Ala acc Thr ggt Gly 315 ggt Gly Val1 Gly gtg Val 140 cgt Arg gag Giu atg Met gcc Ala gtg Vai 220 cta Leu gcc Ala acc Thr atg Met agc Ser 300 gct Ala gcc Ala Asn gca Ala 125 gga Gly ggc Giy ctt Leu gca Ala cgc Arg 205 cag Gin tcc Ser acg Thr ctc Leu cgt Arg 285 caa Gin acc Thr gac Asp Val 115 gc t Ala ga t Asp ggc Gly gag Glu ctc Leu 195 acc Thr gca Ala t cc Ser gg t Gly cca Pro 275 cac His cgc Arg tac Tyr ccc Pro PCT/IBOO/00923 Met Arg gcc att 499 Ala Ile ggc caa 547 Gly Gin gta tgg 595 Val Trp 165 ctt ctc 643 Leu Leu 180 aac agg 691 Asn Arg aaa gcg 739 Lys Ala gaa gca 787 Giu Ala acc aga 835 Thr Arg 245 gtc ttg 883 Vai Leu 260 gag atc 931 Giu Ile tgg act 979 Trp Thr gca gtc Ala Val acc gtg Thr Val 325 atg gca Met Ala 340 1274 WO 01/00843 WO 0100843PCTI/iBOO/00923 acc cgc atg gtg gaa gtg ggg cgc aag tac cca gat gcc aag att cac 1171 Thr Arg Met Val Giu Val Gly Arg Lys Tyr Pro Asp Ala Lys Ile His 345 350 355 ctc tac ggc aag gga cat cgc ccg gga cga aag att ggc cac gtc aac 1219 Leu Tyr Giy Lys Gly His Arg Pro Gly Arg Lys Ile Gly His Val Asn 360 365 370 atg gtg gga tcc gac ctt gaa aag acc cga gcc gaa gcc ctg gcc tgc 1267 Met Val Gly Ser Asp Leu Glu Lys Thr Arg Ala Glu Ala Leu Ala Cys 375 380 385 gca tac ttc ctt gtc aac gct cgc tgg gat taggtctttt ctgagcgcta 1317 Ala Tyr Phe Leu Val Asn Ala Arg Trp Asp 390 395 gca 1320 <210> 930 <211> 399 <212> PRT <213> Corynebacterium glutamicum <400> 930 Val Thr Ser Thr Gly Asn Gin Ala His Ala Pro Gly Met Pro Ilie Val 1 5 10 Ala Vai Ile Giy Asp Gly Gin Leu Ala Arg Met Met Gin Thr Ser Ala 25 Ile Glu Leu Gly Gin Ser Leu Arg Vai Leu Ala Giy Ala Pro Asp Ser 40 Ser Ala Ala Gin Val Ala Ala Asp Vai Val Leu Gly Asp Tyr Thr Asn 55 Ilie Asp Asp Leu Arg Val Ala Ile Glu Gly Ala Asp Val Met Thr Phe 70 75 Asp His Glu His Vai Pro Asn Glu His Leu His Gin Leu Ile Ala Giu 90 Giy Vai Asn Val Gin Pro Arg Pro Glu Ala Leu Vai Asn Ala Gin Asp 100 105 110 Lys Leu Val Met Arg Lys Arg Leu Arg Glu Leu Gly Ala Pro Val Pro 115 120 125 Pro Phe Ala Ala Ilie Giu Ser Val Glu Asp Ala Val Gly Phe Phe Giu 130 135 140 Ala Val Asp Gly.Gin Val Cys Leu Lys Ala Arg Arg Gly Gly Tyr Asp 145 150 155 160 1275 WO 01/00843 Gly Lys Gly Val TrP 165 PCTIBOO/00923 Phe Pro Ala Asp Val Ala Glu Leu Gin Ser Leu 170 175 Val Val Gly Val 225 Gin Val Gly Thr Hius 305 Ala Glu Asp Ile Glu Leu 195 Thr Ala Ser Gly Pro 275 His Arg Tyr Pro Lys 355 His Leu 180 Asn Lys Glu Thr Val 260 Giu Trp Ala Thr Met 340 Ile Val1 Leu Arg Ala Ala Arg 245 Leu Ile Thr Val Val 325 Ala His Asn Leu Val1 Ser Giu 235 Ile Glu Ala Thr Giy 315 Giy Val His Leu Met Ala Val 220 Leu Ala Thr Met Ser 300 Ala Ala G ly Arg Glu 380 Ala Arg 205 Gin Ser Thr Leu Arg 285 Gin Thr Asp Arg Pro 365 Lys Giu Ala Leu Ala Cys Ala Tyr Phe Leu Val Asn Ala Arg Trp Asp 385 390 395 <210> 931 <211> 833 <212> DNA <213> Corynebacteriun giutanicun <220> <221> CDS <222> <223> FRXA02345 <400> 931 tit gct gcc att gaa tca gtc gaa gat gca gtg gga ttc ttc gaa gca 48 Phe Ala Ala Ilie Giu Ser Val Glu Asp Ala Val Gly Phe Phe Glu Ala 1 5 10 1276 WO 01/00843 gtt gat ggc Val Asp Gly aag ggc gta Lys Gly Val gca gag ctt Ala Glu Leu gcc ctc aac Ala Leu Asn gaa acc aaa Giu Thr Lys tgt gca gaa Cys Ala Giu gaa tcc acc Giu Ser Thr 115 act ggt gtc Thr Gly Val 130 cag cca gag Gin Pro Giu 145 ggc cac tgg Gly His Trp, ctc cgc gca Leu Arg Ala gat tac acc Asp Tyr Thr 195 atg ccc atg Met Pro Met 210 gcc aag att Ala Lys Ile 225 ggc cac gtc Gly His Val gcc ctg gcc caa Gin tgg Trp ctc Leu agg Arg gcg Ala gca Ala 100 aga Arg t tg Leu atc Ile act Thr gtc Val 180 gtg Val gca Aia cac His aac Asn tgc tgc Cys cca Pro ggc Giy c tg Leu 70 cca Pro gct Ala t tg Leu gtg Val gtc Val1 150 gat Asp gac Asp gcc Ala cgc Arg tac Tyr 230 gtg Val tac ctc Leu gcc Ala ggc Gly tcc Ser gtc Val ccc Pro gcc Ala gag Giu 135 aac Asn ggc Gly tac Tyr aac Asn atg Met 215 ggc Gly gga Gly t tc gca Ala 25 gta Val1 cca Pro atg Met gaa Giu cct Pro 105 aag Lys ttt Phe c tc Leu gtg Val ctg Leu 185 ctc Leu gaa Giu gga Gly gac Asp gtc cgc Arg gca Ala ctc Leu gtt Val tca Ser gaa Glu atc Ile gaa Giu gcc Ala acc Thr 170 ggt Gly ggt Gly gtg Val cat His ctt Leu 250 aac tac Tyr tcg Ser aag Lys cca Pro aac Asn gaa Giu 110 ctc Leu caa Gin cac His gag Glu act Thr 190 gac Asp tac Tyr cga Arg cga Arg gat PCT/IBOO/00923 gac ggc 96 Asp Gly ctt gtg 144 Leu Val aaa gtt 192 Lys Val agt gga 240 Ser Gly ggt gtg 288 Gly Val ctg cag 336 Leu Gin ggc gtc 384 Gly Val aac ggg 432 Asn Gly aac acc 480 Asn Thr 160 cag cac 528 Gin His 175 ttg gct 576 Leu Ala cca gag 624 Pro Glu cca. gat 672 Pro Asp aag att 720 Lys Ile 240 gcc gaa 768 Ala Giu 255 810 1277 WO 01/00843 Ala Leu Ala Cys Ala Tyr Phe Leu Val Asn Ala Arg Trp Asp 260 265 270 taggtctttt ctgagcgcta gca <210> 932 <211> 270 <212> PRT <213> Corynebacterium glutamicun PCT/iBOO/00923 833 <400> 932 Phe Ala Ala Ile Glu Ser Val Glu Asp 1 Val Lys Ala Ala Glu Cys Glu Thr Gin 145 Gly Leu Asp Met Ala 225 Gly Gly Val Leu Asn Lys Glu Thr 115 Val Glu Trp Ala Thr 195 Met Ile Val Gin Trp Leu Arg Al a Ala 100 Arg Leu Ile Thr Val1 180 Val1 Ala His Asn Val Phe Asp Glu Trp, Ile Gly Ala Phe Gin 165 Leu Met Thr Leu Met 245 Cys Pro Gly Leu 70 Pro Ala Leu Val Val1 150 Asp Asp Ala Arg Tyr 230 Val1 Val Arg Glu Met Ala 75 Val Leu Al a Thr Met 155 Ser Ala Ala Gly Arg 235 Glu Gly Gly Leu Ala Arg Gin Ser Thr Leu 140 Arg Gin Thr Asp Arg 220 Pro Lys Phe Gly Gin Glu Thr Lys Ala Glu 125 Asp Ser Phe Asp Thr 205 Lys Gly Thr Phe Tyr Ser Lys Pro Asn Glu 110 Leu Gin His Glu Thr 190 Asp Tyr Arg Arg Ala Gly Val Val Gly Val Gin Val1 Gly Thr 160 His Ala Glu Asp Ile 240 Glu Ala Leu Ala Cys Ala Tyr Phe Leu Val Asn Ala Arg Trp Asp 1278 WO 01/00843 WO 0100843PCT/1B00100923 <210> <211> <212> <213> <220> <221> <222> <223> 260 933 618

DNA

Corynebacteriun glut am icum

CDS

(101)..(595) RXN02 350 <400> 933 aagcatttct ccaacacttt tgatggacag attaacgctt aaaaggcctg ttatagactg ataaaccgat acgtactttt cgcgcttaag gagaatttca gtg ggt cct cta Val Gly Pro Leu 1 ggt ttg atc atg Gly Leu Ile Met tcg gat tcg gat Ser Asp Ser Asp gac act gta gcg Asp Thr Val Ala cca gct Pro Ala gca gag gta Ala Glu Val tct gca cac Ser Ala His ctc Leu gct gag ttt ggc Ala Glu Phe Gly cct ttt gaa gtc Pro Phe Glu Val gga gtt gtc Gly Val Val aag act gca Lys Thr Ala cgc acc cca gag *Arg Thr Pro Glu atg ctc aac tac Met Leu Asn Tyr gca Ala cat gag His Glu cgc ggc atc aag Arg Gly Ile Lys atc atc gcg tgt Ile Ile Ala Cys ggc ggc gct gca Gly Gly Ala Ala cac His ctg cca ggc atg gtg gct gca gca act Leu Pro Gly Met Val Ala Ala Ala Thr ctt cca gtc atc Leu Pro Val Ile gtt cca cgc gca Val Pro Arg Ala aag gat ctc gac Lys Asp Leu Asp ttg gat tcc ttg Leu Asp Ser Leu ctg tcc Leu Ser 100 atc gtc cag Ile Val Gin ggc gcg aag Gly Ala Lys 120 cca ggc ggc gtt cca gtc gcc act gtc Pro Gly Gly Val Pro Val Ala Thr Val 110 tcc atc ggt Ser Ile Gly 115 ggt gct ggt Gly Ala Gly aat gca ggc cta Asn Ala Gly Leu gcc gtt cgt att Ala Val Arg Ile gat cct Asp Pro 135 tct ttg gtc acg Ser Leu Val Thr atg gcc gat Met Ala Asp tac caa Tyr, Gin 145 aag aag Lys Lys 160.

gag aat atg gcg Glu Asn Met Ala aag Lys 150 gaa gtt gaa gcc Glu Val Glu Ala aag Lys 155 gac gaa gca ctg Asp Glu Ala Leu cgc ttg ctc Arg Leu Leu ggc Gly 165 taatgaatcc gatcgtggtg ctg 1279 WO 01/00843 WO 0100843PCT/iBOO/00923 <210> 934 <211> 165 <212> PRT <213> Corynebacterium glutamicun <400> 934 Val Gly Pro Leu Val Gly Leu Ile Met 1 Thr Giu Tyr Ala Leu Asp Thr Ile Gin 145 Lys Ala Ser His His 70 Val1 Ile Gly Asp Lys 150 Glu Ala Glu Leu Pro Vali Ala Pro 135 Gi u Val His Arg Pro Arg Gin Lys 120 Ser Val Gly 10 Ala Thr Ile Met Leu Pro Ala Vali Ala Ser Giu Pro Lys Val 75 Lys Giy Gly Thr Lys 155 Ser Gly Lys Ile Ala Leu Vali Leu 125 Met Giu Asp Ile Met Ile Ala Asp Pro 110 Ala Ala Ala Trp Asp Pro Phe Leu Asn Ala Cys Thr Pro Gly Leu Vai Ala Val Arg Asp. Tyr Leu Lys 160 <210> 935 <211> 223 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (101) (223) <223> FRXA02346 <400> 935 aagcatttct ccaacacttt tgatggacag attaacgctt aaaaggcctg ttatagactg ataaaccgat acgtaacttt cgcgcttaag gagaatttca gtg ggt cct cta gtt 115 Val Gly Pro Leu Val 1 ggt ttg atc atg ggt tcg gat tcg gat tgg gac act gta gcg cca gct 163 Giy Leu Ile Met Gly Ser Asp Ser Asp Trp Asp Thr Val Ala Pro Ala 1280 WO 01/00843 WO 0100843PCT/EBOO/00923 gca gag gta Ala Giu Val ctc gct gag ttt ggc att cct Leu Ala Glu Phe Gly Ile Pro 30 ttt gaa gtc Phe Giu Val gga gtt gtc Gly Val Val tct gca cac cgc 223 Ser Ala His Arg <210> 936 <211> 41 <212> PRT <213> Corynebacterium glutamicum <400> 936 Val Gly Pro Leu Val Gly Leu Ilie Met Gly 1 5 10 Ser Asp Ser Asp Trp Asp Thr Val Ala Pro Ala Ala Glu Val Leu 25 Glu Val Gly Val Val Ser Ala His Arg <210> 937 <211> 252 <212> DNA <213> Corynebacterium glutanicui Ala Giu Phe Gly Ile Pro Phe <220> <221> <222> <223>

CDS

(20)..(229) FRxAO2350 <400> 937 atactaggat ctcgacggtttg gat tcc ttg Leu Asp Ser Leu

I

tcc atc gtc cag Ser Ile Val Gin atg cca Met Pro ggc ggc gtt Gly Gly Val ggc cta ctt Gly Leu Leu gtc gcc act gtc Val Ala Thr Val atc ggt ggc gcg Ile Gly Gly Ala aag aat gca Lys Asn Ala tct ttg gtc Ser Leu Val gcc gtt cgt att Ala Val Arg Ile ggt gct ggt gat Gly Ala Gly Asp acg aag Thr Lys atg gcc gat tac Met Ala Asp Tyr caa gag Gin Glu 50 aat atg gcg Asn Met Ala aag Lys gaa gtt gaa gcc Glu Val Glu Ala gac gaa gca ctg Asp Glu Ala Leu aag cgc ttg ctc ggc Lys Arg Leu Leu Gly taatgaatcc gatcgtggtg 249 1281 WO 01/00843 PCT/EB <210> 938 <211> <212> PRT <213> Corynebacterium giutamicun <400> 938 Leu Asp Ser Leu Leu Ser Ilie Val Gin Met Pro Gly Gly Val Pro Val 1 5 10 Ala Thr Val Ser Ile Gly Gly Ala Lys Asn Ala Gly Leu Leu Ala Val 25 Arg Ilie Leu Gly Ala Gly Asp Pro Ser Leu Val Thr Lys Met Ala Asp 40 Tyr Gin Glu Asn Met Ala Lys Glu Val Giu Ala Lys Asp Glu Ala Leu 55 Lys Lys Arg Leu Leu Gly <210> 939 <211> 999 <212> DNA <213> Corynebacteriun glutainicum <22 0> <221> CDS <222> (976) <223> RXA01087 <400> 939 ggccttggca gagaactcgg ccccgaaggt cttgcttcct accaagaaac ccaaacca tatctctaat ccaaactgca cctatatata aggagtaaaa atg caa acc ctt gct 00/00923 tt gc t Ala ctg Leu gga Gly ttt Phe gtg Val gga Gly att Ile gat Asp gtg Val ctt Leu ggt Gly t tc Phe gtt Val gca Ala cgc Arg ccc Pro tct Ser aac Asn cctI ProI gtt Val gtt Val gaa4 Giu aaa Lys I ttg4 Leu 1282 gag Glu atc Ile gat Asp gcc Ala gat Asp 80 tgt Cys Met Gin Thr Leu Ala 1 atc acc acc att gat Ile Thr Thr Ile Asp cgt gtt att gct gcc Arg Val Ile Ala Ala cag att tac cca act Gin Ile Tyr Pro Thr gga gta gtt gtc gcc Gly Val Vai Val Ala gac aag gta gtg ctg Asp Lys Val Val Leu ttg ggc ggt aag cct Leu Gly Gly Lys Pro WO 01/00843 WO 0100843PCTIBOOIOO923 100 gcg tac tgt Ala Tyr Cys gcc ggg cac Ala Gly His 120 aaa ttc tat gac Lys Phe Tyr Asp aac ttc gca tgc Asn Phe Ala Cys acc cgc gat Thr Arg Asp 115 cag gca gag Gin Ala Glu act act ttg ttt Thr Thr Leu Phe acc Thr 125 cgt gca aca aaa Arg Ala Thr Lys gag Giu 130 gcc atc Ala Ile 135 atc gac acc ctt Ilie Asp Thr Leu gat Asp 140 gat gtt ttc tac Asp Val Phe Tyr gcg gat gcg ggt Ala Asp Ala Gly ttc Phe 150 ctg gca tac cca Leu Ala Tyr Pro act ccc cca gag Thr Pro Pro Giu tcg gga gta agc Ser Gly Val Ser ttg gtt gtc gcg Leu Val Val Ala ggt acc tct gat Giy Thr Ser Asp ccc caa gca aag Pro Gin Ala Lys gaa gca Giu Ala 180 cta cac act Leu His Thr tet gga gtg Phe Gly Val 200 tcc tac ttg ggg Ser Tyr Leu Gly tcc acc tca ctg Ser Thr Ser Leu att gtt gat Ile Vai Asp 195 gaa gaa ctc Giu Giu Leu gct ggc atc cac Ala Gly Ilie His cgc Arg 205 ctg ctt tca tac Leu Leu Ser Tyr cgc gct Arg Aia 215 gcg ggc gtg ctc Ala Gly Val Leu atc Ile 220 gtt gcc gct gga Val Ala Ala Giy gat ggt gcg cta Asp Gly Ala Leu ccc Pro 230 gga gtt gtc gca Gly Val Val Ala tta gtg tcc gca Leu Val Ser Ala gtc gtc gca ctg Val Val Ala Leu cca Pro 245 acc tcc gtg gga Thr Ser Val Gly ggc gca ggt gct Gly Ala Gly Ala gga atc gca cca Gly Ile Ala Pro ctt ctg Leu Leu 260 acc atg ctt Thr Met Leu aac ggc tat Asn Gly Tyr 280 gcc tgc gcg ccg Ala Cys Ala Pro gga Gly 270 gtt gga gtg gtc Val Gly Val Val aac att gat Asn Ile Asp 275 gga gca gga cac Gly Ala Gly His ctg Leu 285 gct gcg cag att Ala Ala Gin Ile gcg gcg agg Ala Ala Arg 290 taagggtttc gcaggagacg aac <210> 940 <211> 292 <212> PRT <213> Corynebacteriun giutanicum <400> 940 Met Gin Thr Leu Ala Ala Ilie Val Arg Ala Thr Lys Gin Pro Phe Glu 1283 WO 01/00843 PCT/IB00/00923 Ile Thr Thr Ile Asp Leu Asp Ala Pro Arg Pro Asp Glu Val Gin Ile 25 Arg Val Ile Ala Ala Gly Val Arg His Thr Asp Ala Ile Val Arg Asp 40 Gin Ile Tyr Pro Thr Phe Leu Pro Ala Val Phe Gly His Glu Gly Ala 55 Gly Val Val Val Ala Val Gly Ser Ala Val Thr Ser Val Lys Pro Asp 70 75 Asp Lys Val Val Leu Gly Phe Asn Ser Cys Gly Gin Cys Leu Lys Cys 90 Leu Gly Gly Lys Pro Ala Tyr Cys Glu Lys Phe Tyr Asp Arg Asn Phe 100 105 110 Ala Cys Thr Arg Asp Ala Gly His Thr Thr Leu Phe Thr Arg Ala Thr 115 120 125 Lys Glu Gin Ala Glu Ala Ile Ile Asp Thr Leu Asp Asp Val Phe Tyr 130 135 140 Asp Ala Asp Ala Gly Phe Leu Ala Tyr Pro Ala Thr Pro Pro Glu Ala 145 150 155 160 Ser Gly Val Ser Val Leu Val Val Ala Ala Gly Thr Ser Asp Leu Pro 165 170 175 Gin Ala Lys Glu Ala Leu His Thr Ala Ser Tyr Leu Gly Arg Ser Thr 180 185 190 Ser Leu Ile Val Asp Phe Gly Val Ala Gly Ile His Arg Leu Leu Ser 195 200 205 Tyr Glu Glu Glu Leu Arg Ala Ala Gly Val Leu Ile Val Ala Ala Gly 210 215 220 Met Asp Gly Ala Leu Pro Gly Val Val Ala Gly Leu Val Ser Ala Pro 225 230 235 240 Val Val Ala Leu Pro Thr Ser Val Gly Tyr Gly Ala Gly Ala Gly Gly 245 250 255 Ile Ala Pro Leu Leu Thr Met Leu Asn Ala Cys Ala Pro Gly Val Gly 260 265 270 Val Val Asn Ile Asp Asn Gly Tyr Gly Ala Gly His Leu Ala Ala Gin 275 280 285 Ile Ala Ala Arg 290 <210> 941 <211> 1551 <212> DNA <213> Corynebacterium glutamicum 1284 WO 01/00843 WO 0100843PCTIiBOO/00923 <220> <221> CDS <222> (101)..(1528) <223> RXA00619 <400> 941 tcaaaggaag acaccattga aggtgtgcgc aaaatcggag aattcatcaa aaaatagcag cgactaggtt agtttcggtt cgtggggaat aataatttgc gtg gct gat aaa Val Ala ASP LYS aag atc gca aac gtc ctg tcg aac cgt Lys Ile Ala Asn Vai Leu Ser Asn Arg gcc tcc gcg gaa Ala Ser Ala Giu ctt tct Leu Ser aac ctg tgg Asn Leu Trp atc gcc gtg Ile Ala Val gcc gaa gag aag Ala Giu Giu Lys atc atg gag cgc Ile Met Giu Arg cag ctc tgg Gin Leu Trp atc cct gcc Ile Pro Ala atg aaa gcc cag Met Lys Ala Gin gat ttg gga gtt Asp Leu Gly Val gag gca Giu Ala att gaa tcc tat Ile Giu Ser Tyr gca gtg atc gac Ala Val Ile Asp cag Gin gtt gat ttg gca Val Asp Leu Ala atc gcc gat cgt Ile Ala ASP Arg cgc gtc *acc cgc Arg Val. Thr Arg gat gtg aag gcc Asp Val Lys Ala atc gaa gaa ttc Ile Giu Giu Phe atg acc tcc cgc Met Thr Ser Arg 105 aat Asn gca ctg gct ggc Ala Leu Ala Gly gag cac atc cac Giu His Ile His aag ggc Lys Gly 100 gac ctc acc gaa Asp Leu Thr Giu gtt gaa cag ctg cag atc cac Vai Giu Gin Leu Gin Ile His 115 cgc tcc ctt Arg Ser Leu 120 gag ctg gtc cgc Giu Leu Va1 Arg aag ggc att gca Lys Gly Ile Ala gtt Val1 130 gtt gca gct Val Ala Ala atc gga Ile Gly 135 tcc cgc gca gcg Ser Arg Ala Ala tac caa agc ctg Tyr Gin Ser Leu atg gct ggc cgt Met Ala Gly Arg cac aac gtg gca His Asn Val Ala gcg Ala 155 cag gca act acc Gin Ala Thr Thr ggc aag cgt ttc Gly Lys Arg Phe acc gca gct gat Thr Ala Ala Asp atg ctc gtg gca Met Leu Val Ala gag cgc gtc acc Giu Arg Vai Thr gaa ctg Giu Leu 180 ctc aac cgc Leu Asn Arg cca ctt cgc gga Pro Leu Arg Gly atc Ile 190 aag ggc cca atg Lys Gly Pro Met gga acc gcc Gly Thr Ala 195 caa gac atg ctt gac ctc atg gaa ggc gac gag gct cgt ctc tcc gat 1285 WO 01/00843 Gin Asp Met 200 ctg gaa acc Leu Giu Thr PCTIiBOO/00923 Leu Asp Leu Met Giu 205 cac His Gly Asp Giu Ala Arg 210 cgc Arg Leu Ser Asp gtc ttc gac Val Phe Asp cgc atc gca Arg Ile Ala gca Ala 220 ctc ggc ttt Leu Gly Phe 215 tcc gtc Ser Val gat Asp 225 ttc Phe ggc cag gtc Gly Gin Vai 230 gc t Ala tac Tyr 235 ggC Gly cca cgt tcc ctt Pro Arg Ser Leu gac Asp 240 tcg Ser gat gca gta Asp Ala Val tct Ser 245 ctg gtt cag Leu Val Gin ctt Leu 250 ggC Gly tcc ggc cca Ser Gly Pro ctg tcc cac Leu Ser His acc att Thr Ile 260 cgt ctc atg Arg Leu Met cag gtc ggt Gin Val Gly 280 gcc Ala 265 tcC S er acc gaa act Thr Giu Thr gaa ggt ttt Giu Giy Phe aag gaa ggc Lys Giu Gly 275 tct gca atg Ser Ala Met cc t Pro 285 gt t Vali aag atg Lys Met gag cgc 1027 Giu Arg 295 gtt gct 1075 Vai Ala 310 tcc gtg 1123 Ser Val gga atg 1171 Gly Met cct gcc 1219 Pro Ala act acc 1267 Thr Thr 375 acc gca 1315 Thr Ala 390 atg cgc 1363 Met Arg gtg ggc ggc ctg Val Gly Gly Leu gat ctt tcc ggc Asp Leu Ser Gly 315 atc cgc cgc gtt Ilie Arg Arg Val 330 ttt gaa act ttc Phe Giu Thr Phe att ctt cgc Ile Leu Arg cag cag tgg aac gaa Gin Gin Trp, Asn Giu 320 gca ctg cca gac gca Ala Leu Pro Asp Ala 335 ctg aca gtc ctg.gat Leu Thr Val Leu Asp aac gct cgc tcc tgt Asn Ala Arg Ser Cys 290 gga tac ctc acc atg Giy Tyr Leu Thr Met 305 ggc gat gtc ttc tgc Gly Asp Vai Phe Cys 325 ttc ttc gcg att gac Phe Phe Ala Ile Asp 340 gaa ttc ggt gca ttc Giu Phe Gly Ala Phe 345 atg atc Met Ile 350 gag cgc gaa ctt gag Giu Arg Giu Leu Giu 365 ctc atg gcc gct gtc Leu Met Ala Ala Val 355 cgt tac ctg cca ttc ctg gca Arg Tyr Leu Pro Phe Leu Ala 370 cgc gca ggc gtt ggc cgc gaa Arg Ala Gly Val Giy Arg Giu atc Ile 380 385 cac gaa gta atc His Giu Val Ilie 395 gaa aat ggc ggt Giu Asn Giy Gly aag gaa aac gct gtc Lys Giu Asn Ala Vai 400 gac cag gac ctt atc Asp Gin Asp Leu Ile gcg gtt gcc ctc aac Ala Val Ala Leu Asn 405 cag cgc ctc gct gct Gin Arg Leu Ala Ala 1286 WO 01/00843 WO 0100843PCTJIBOO/00923 410 gat gag cgc ctc cca atg agc gaa gct 1411 Asp Glu Arg Leu Pro Met Ser Glu Ala 425 430 gac cgc cac gcc ttc atc ggt gcc gct 1459 Asp Arg His Ala Phe Ile Gly Ala Ala 440 445 ctc gac cgc att cag gtg ctt gtc gac 1507 Leu Asp Arg Ile Gin Val Leu Val Asp 455 460 tac cga cca ggt gag att ctt taaaggtt 1551 Tyr Arg Pro Gly Glu Ile Leu 470 475 <210> 942 <211> 476 <212> PRT <213> Corynebacterium giutanicun <400> 942 Val Ala Asp Lys Lys Lys Ile Ala Asn 1 Ser Ala Giu Leu Ser Asn Leu Trp Ser Glu Arg Gin Leu Trp Ile Ala Val Met Val Glu Ile Pro Ala Glu Ala Ile Glu 55 Gin Val Asp Leu Ala Ser Ile Ala Asp 70 Asp Val Lys Ala Arg Ile Giu Glu Phe His Ile His Lys Gly Met Thr Ser Arg 100 105 Gin Leu Gin Ile His Arg Ser Leu Glu 115 120 Ala Val Val Ala Ala Ile Giy Ser Arg 130 135 Val Met Ala Gly Arg Ser His Asn Val 145 150 Gly Lys Arg Phe Ala Thr Ala Ala Asp 165 415 gac ctt gag gca gcg Asp Leu Giu Ala Ala 435 gag tcc cag gtc tcc Glu Ser Gin Val Ser 450 gcc cac ccc ggc gcc Ala His Pro Gly Ala 465 :tt taacggcgtt cac 420 ctg gct Leu Ala cgt gtg Arg Val gca gac Ala Asp Leu Giu Ala Tyr Glu Ala Leu Val Ala Ala 155 Met Ser Giu Gin Giu Arg Leu Thr Arg Gin 140 Gin Leu 1287 WO 01/00843 WO 0100843PCT/EBOOIOO923 Arg Val Thr Glu Leu Leu Asn Arg 180 Pro Ala Asp 225 Phe Leu Gly Asn Gly 305 Giy Phe Giu Leu Giy 385 Ala Gin Giu Gin Gly 195 Leu Val Ala His Lys 275 Arg Leu Val1 Ala Gly 355 Phe Gly Ala Leu Ala 435 Ser Thr Ala Ser Asp Phe Asp Val Ser 245 Thr Ile 260 Glu Gly Ser Cys Thr Met Phe Cys 325 Ilie Asp 340 Ala Phe Leu Ala Arg Giu Leu Asn 405 Ala Ala 420 Leu Ala Arg Val Gin Leu Ser 230 Ala Arg Gin Giu Val 310 Ser Gly Pro Thr Thr 390 Met Asp Asp Leu Asp Met 200 Giu Thr 215 Val Giy Leu Val Leu Met Val Gly 280 Arg Val 295 Ala Asp Val Ile Met Phe Ala Met 360 Thr Arg 375 Ala His Arg Giu Giu Arg Arg His 440 Asp Arg 455 Tyr 185 Leu Arg Gin Gin Ala 265 Ser Gly Leu Arg Giu 345 Ile Ile Giu Asn Leu 425 Ala Ile Pro Leu Arg Gly Ile Lys Gly 190 Asp Ile Val Leu 250 Gly Ser Gly S er Arg 330 Thr Giu Leu Val Gly 410 Pro Phe Gin Leu Ala Tyr 235 Gly Thr Ala Leu Gly 315 Val1 Phe Arg Met Ile 395 Gly Met Ile Val Met Ala 220 Pro Ser Giu Met Gin 300 Gin Ala Leu Giu Al a 380 Lys Asp Ser Gly Leu 460 Glu 205 His Arg Gly Thr Pro 285 Val Gin Leu Thr Leu 365 Ala Giu Gin Giu Ala 445 Val Gly Leu Ser Pro Val 270 His Ile Trp Pro Val 350.

Giu Val Asn Asp Ala 430 Ala

ASP

Asp Giy Leu Ser 255 Thr Lys Leu Asn Asp 335 Leu Arg Arg Ala Leu 415 Asp Giu Ala Giu Phe Asp 240 Ser Giu Met Arg Giu 320 Ala Asp Tyr Ala Val 400 Ile Leu Ser His Pro Gly Ala Ala Asp Tyr Arg Pro Gly Giu Ile Leu <210> 943 <211> 1683 1288 WO 01/00843 WO 0100843PCTUBOO/00923 <212> DNA <213> Corynebacterium giutaniicum <220> <221> CDS <222> (1660) <223> RxA02622 atcaagcagg ttgagcgtaa actcattgta gaagtcctga cagggtggcg tacaactcaa ctggagaggc taaatccttc acagcgtgga attttcgcgt atg agc gat gat cgt 1i Met Ser Asp Asp Arg 1 aag gca att aaa Lys Ala Ile Lys gca cta att agc Ala Leu Ile Ser tat gac aag act Tyr Asp Lys Thr ggc ctg Gly Leu gag gat cta gcc cag gca ctt cac Glu Asp Leu Ala Gin Ala Leu His gag aac gtg gaa Glu Asn Val Glu att gtt tcc Ile Val Ser cct gtt acc Pro Val Thr acc gga tcc Thr Gly Ser act gcg gcg aag Thr Ala Ala Lys att Ile gct gag ctt ggt Ala Glu Leu Gly ccg gtt Pro Val gag gag ctc acc Glu Giu Leu Thr ttc cct gag tgc Phe Pro Giu Cys ctt Leu gag ggc cgt gtg Giu Gly Arg Val aca ctg cac cct Thr Leu His Pro gtt cac gct ggc Val His Ala Gly ttg gcg gac acc Leu Ala Asp Thr aag gaa gac cac Lys Glu Asp His ctg Leu cgt cag ctc aag Arg Gin Leu Lys ctt gag gtc gcc Leu Giu Val Ala cca ttc Pro Phe 100 cag ctt gtc Gin Leu Val ggc gcc gat Gly Ala Asp 120 gtg aac ctg tac Val Asn Leu Tyr ttt gct gag acc Phe Ala Giu Thr gtt gcg tcc Val Ala Ser 115 gga ggc cca Gly Gly Pro ttc gat gct tgc Phe Asp Ala Cys gag cag atc gac Giu Gin Ile Asp tcc atg Ser Met 135 gtt cgt gct gcg Val Arg Ala Ala gca Ala 140 aag aac cac cca tct gtc gct gtg gtt Lys Asn His Pro Ser Val Ala Val Val 145 tca ccg aac cgc Ser Pro Asn Arg t ac Tyr 155 gag gat gtc cag Giu Asp Val Gin gct ttg aag acc Ala Leu Lys Thr gga ttc tcc cgc Gly Phe Ser Arg gag cgc acc aag Giu Arg Thr Lys gct gct gag gct Ala Ala Glu Ala ttc cgc Phe Arg 180 cac acc gca acc tac gat gtc acc gtt gca acc tgg atg agc gag cag His Thr Ala Thr Tyr Asp Val Thr Val Ala Thr Trp Met Ser Glu Gin 1289 WO 01/00843 WO 0100843PCT/IIBOO/00923 ctg gct Leu Ala acc aac Thr Asn 215 tct gca Ser Ala 230 cag ttc Gin Phe gct gca Ala Ala atc aag Ile Lys gca gcg 1027 Ala Ala 295 ggt ggc 1075 Gly Gly 310 cag gtt 1123 Gin Vai gag ggc 1171 Giu Giy cag gct 1219 Gin Ala ggc ggt 1267 Gly Gly 375 aac tcc 1315 Asn Ser 390 gcc Ala 200 acc Thr gct Ala cac His tgg Trp cat His 280 gca Ala gtc Val gca.

Aia gc t Ala gaa Glu 360 ctg Leu gca Ala gaa Glu ttg Leu ttg Leu ggc Gly cgt Arg 265 gca Ala cac His atc Ile gag Glu gtg Val1 345 gca Ala ctt Leu aac Asn gat Asp tcc Ser tac Tyr aag Lys 250 gca Ala aac Asn cgc Arg gcg Ala atc Ile 330 gag Glu cct Pro gtt Val1 tgg Trp tct Ser cgc Arg gtg Val1 235 gaa Giu gcg Ala cct Pro gag Giu tcc Ser 315 ttc Phe atc Ile gtg Val cag Gin act Thr 395 gag Giu agc Ser 220 ggc G ly atg Met tgg Trp tgt Cys gca Ala 300 aac Asn act Thr ctg Leu cg t Arg gaa Giu 380 ctt Leu t tc Phe tac Tyr cgc Arg aac Asn 255 gag Giu gc t Ala tgt Cys gtc Val1 atc Ile 335 aag Lys ttt Phe ttg Leu ggc Gly cca Pro ggt Gly gga Gly 240 aac Asn cgt Arg gtt Val gac Asp agc Ser 320 atc Ile aag Lys gag Giu atc Ile tct Ser 400 ggt Gly gag Glu 225 ctt Leu tac Tyr cct Pro tct Ser tct Ser 305 gt t Val gc t Al a aac Asn tcc Ser cac His 385 gc t Ala 195 atc Ile cc t Pro cag Gin gat Asp gta Val1 275 gag Giu tcc Ser atg Met tcc Ser cgt Arg 355 gag Glu gag Glu tct Ser 1290 WO 01/00843 gtt ctg aag gac 1363 Val Leu Lys Asp tcc aac gca att 1411 Ser Asn Ala Ilie 425 atg gga cag gtc 1459 Met Gly Gin Val 440 gca ggt gca gag 1507 Ala Gly Ala Glu 455 ttc cca ttc gct 1555 Phe Pro Phe Ala 470 gct gtt gtg cag 1603 Ala Val Val Gin gca gcc aac aag 1651 Ala Ala Asn Lys 505 ttc gct cac taa 1683 Phe Ala His 520 gag Glu ttg Leu cgc Arg gct Ala 9gc Gly 475 ggt Gly ggc ttc Phe gct Ala gtt Val acc Thr 460 ttt Phe gga Gly gtg gcg Ala aag Lys gac Asp 445 ggt Gly gag Giu tcC Ser acc tgg Trp aac Asn 430 tct Ser tcc Ser gtt Val att Ile atg gca Ala gc t Ala cgc Arg gct Ala gc t Al a 480 gac Asp ctg Leu tcc Ser 9gC Gly 435 gtc Val1 gat Asp ggc Gly gtc Val1 geg Ala 515 PCTIBOO/00923 gtg aag Val Lys 420 gtt ggc Val Gly gac cgt Asp Arg gcg ttc Ala Phe atc act Ile Thr 485 att gag Ile Giu 500 cga cac Arg His Ala Gly Val Thr Met Tyr 510 <210> 944 <211> 520 <212> PRT <213> Corynebacterium glutainicum <400> 944 Met Ser Asp Asp Arg Lys Ala Ile I 1 5 Asp Lys Thr Gly Leu Giu Asp Leu 2 Val Glu Ile Val Ser Thr Gly Ser 'j 40 Gly Ilie Pro Val Thr Pro Val Giu 55 Leu Giu Gly Arg Val Lys Thr Leu 1 70 Leu Ilie Ser Val Tyr Leu His Arg Giu Asn Lys Ile Ala Giu Leu Gly Phe Pro Giu Cys Val His Ala Gly Ile 1 291 WO 01/00843 WO 0100843PCTIIBOO/00923 Leu Glu Glu Asp Ser 145 Ala Al a Trp Gly Glu 225 Leu Tyr Pro Ser Ser 305 Val1 Ala Asn Ser His 385 Ala Val1 Thr Ile 130 Val1 Leu Giu Met Trp 210 Asn Ala Thr Cys Asp 290 Val1 Glu Pro Ile Arg 370 Ala Asp Ala Val 115 Gly Ala Lys Ala Ser 195 Ile Pro Gin Asp Val 275 Glu Ser Met Ser Arg 355 Glu Glu Thr Pro 100 Ala Gly Val1 Thr Phe 180 Glu Gly His Ala Ser 260 Al a Ser Ala Ala Tyr 340 Ile Ile Gly Arg Phe Ser Pro Val1 Gly 165 Arg Gln Thr Gin Lys 245 Asp Ile Ile Phe Asn 325 Glu Leu Ser Asp Lys Gln Gly Ser Val 150 Giy His Leu Thr Ser 230 Gin Aia Ile Aia Giy 310 Gin Glu Gln Giy Asn 390 Glu Leu Ala Met 135 Ser Phe Thr Ala Asn 215 Ala Phe Ala Lys Ala 295 Gly Val G ly Al a Gly 375 Ser Asp Val1 Asp 120 Val1 Pro Ser Ala Al a 200 Thr Ala His Trp His 280 Ala Val1 Ala Ala Glu 360 Leu Ala Leu Val Asp Ala Arg Ala 170 Tyr Asp Ser Tyr Ly s 250 Ala Asn Arg Ala Ile 330 Glu Pro Val Trp Arg Asn Ala Ala Tyr 155 Giu Asp Ser Arg Val 235 Giu Ala Pro Glu Ser 315 Phe Ile Val1 Gin Thr 395 Leu Tyr Val 125 Lys Asp Thr Thr Thr 20.5 Leu Asn Ser Asp Gly 285 His Arg Glu Ser Lys 365 Arg Ala Lys Pro 110 Giu Asn Val1 Lys Val 190 *Giu Arg Thr Tyr His 270 Ile Ala Glu Val1 Gin 350 Gly Asp Ala 1292 WO 01/00843 Ala Val Se Vai Arg SE Thr Val GI 43 Leu Ala Va 450 Ala Ser As 465 Glu Ala GI Asn Giu Va Thr Gly Al Leu Asn Gly Gly 455 Pro Val1 Ala Ala Asp Ile 425 Val Giu Ala Gin Lys 505 Trp Asn 430 Ser Ser Val Ile Met 510 PCTIBOO/00923 Thr Ala 415 Gly Ala Ala Arg Val Ala Leu Ala 480 Arg Asp 495 Tyr Leu <210> 945 <211> 1641 <212> DNA <213> Corynebacterium glutarnicum <220> <221> CDS <222> (1618) <223> RXN00488 <400> 945 gttgtggaat agcgtgtgag ctgcagcggt tatccgattt tagataattg tggacacact atgaggagga actgtcgcac cga Arg tta Leu cct Pro aac Asn atg Met gga Gly gta.

Val acc Thr tct Ser gca Ala 75 gac Asp ctt Leu tcg Ser gcc Ala cgC Arg aac Asn ttg Leu acg Thr gca Ala cat His aaggacaagc tactaaagtt atg aca acc cag agc Met Thr Thr Gln Ser 1 gtt gcc ctt gtt gga Val Ala Leu Val Gly gcg tcg gac gtt gtt Ala Ser Asp Val Val cgt aat att cgc ctt Arg Asn Ile Arg Leu gtc acc gag gct cgc Val Thr Glu Ala Arg ggt gtt ttg cac cgc Gly Val Leu His Arg 115 163 211 259 307 355 1293 WO 01/00843 WO 0100843PCTIBOO/00923 aac ctg tct att caa Asn Leu Ser Ilie Gin cag gca gaa aac gtt gag ctg Gin Ala Giu Asn Val Giu Leu 95 tct gag Ser Giu agc atc Ser Ile ctg cct Leu Pro 135 cgc gat Arg Asp 150 atg acc Met Thr cag gct Gin Ala atc gca Ile Ala gtt aag Val Lys 215 ctg ctg Leu Leu 230 gct ggt Ala Gly gca cac Ala His aag tcg Lys Ser gag gct 1027 Giu Ala 295 ggt att 1075 Gly Ilie gga Gly 105 gaa Giu gtt Val cgt Arg atg Met gc t Ala 185 gac Asp gag Giu gcg Ala ctt Leu cat His 265 ccc Pro cag Gin cca Pro atg Met gtg 'Val1 gat Asp t tt Phe cct Pro* 170 t tg Leu ggc Gly cag Gin gct Ala gcc Ala 250 agc Ser aag Lys gcc Ala ggt Gly g tc Val1 gat Asp gag Glu gaa Glu 155 ttg Leu ctg Leu aag Lys c ac His ggc Giy 235 gac Asp cgt Arg gtc Val atg Met tct S er act Thr gat Asp gcc Ala 140 agc Ser gtt Val tc t Ser ct t Leu ccg Pro 220 atc Ilie gcc Al a gga Gly gat Asp att Ile 300 at t Ile gat Asp ctg Leu 125 gga Gly gac Asp gtt Val1 gca Ala gtc Val1 205 aac Asn ggc Gly ggc Gly gtt Val a tc Ile 285 gaa Glu tgc Cys cctI Pro 110 tgt Cys aag Lys atg Met gct Ala aac Asn 190 ggt Gly I gca Ala acgI Thr gtc Val ttg Leu 270 gtt Val gct Ala accj Thr 1294 acc Thr cgc Arg gtt Val cgt Arg 160 gag Giu gtg Val1 atc Ile aag Lys gag Giu 240 att Ile atg Met 99C Gly gca Ala cgc Arg tgt Cys t tc Phe ggt Gly 145 cgt Arg ggc Gly gag Gi u acg Thr ga t Asp 225 gag Giu ttg Leu gtg Val1 aac Asn gac Asp 305 gtt Val1 act Thr cgc Arg 130 att Ile gtc Val gtc Val aag Lys gtg Val1 210 gca Ala tca Ser gtc Val tcc Ser ttg Leu 290 gc t Ala gtc Val1 gtg aag Val Lys 100 cct gac Pro Asp 115 att tcc Ile Ser tgc acc Cys Thr gct gaa Ala Giu acc aag Thr Lys 180 ctt cct Leu Pro 195 aag gac Lys Asp tca ggt Ser Gly ttc cag Phe Gin gta gac Val Asp 260 cgc gtg Arg Val 275 gcg acc Ala Thr atc aag Ile Lys gca ggt Ala Giy cg t Arg atg Met ggt Gly aac Asn gtt Vai 165 gag Glu atc Ile t tc Phe cgt Arg cga Arg 245 tct Ser aag Lys cgc Arg gtg Vali gtc Val 403 451 499 547 595 643 691 739 787 835 883 931 979 WO 01/00843 WO 0100843PCTIBOOIOO923 310 ggt gca 1123 Gly Ala aag gct 1171 Lys Ala gat atc 1219 Asp Ile tcc atg 1267 Ser Met 375 aac ggc 1315 Asn Gly 390 atg cag 1363 Met Gin cgt tac 1411 Arg Tyr ggc atc 1459 Gly Ile cct cag atc Pro Gin Ilie 330 315 act Thr atc Ile gcg atc atg Ala Ile Met atc gcc gat Ile Aia Asp gag Giu 335 325 gct gtt cca gct cac Ala Val Pro Aia His 340 ggc gtt Gly Vai 345 gct aag Aia Lys 360 cc t Pro ggc ggc atg cag ttc tct ggt Giy Gly Met Gin Phe Ser Giy 355 350 gct ttg gct gct Ala Leu Ala Ala 365 ggc Gly gct aac tcc Ala Asn Ser ctg gct ggt acc Leu Ala Giy Thr aag cag tac aag Lys Gin Tyr Lys gc t Ala 380 gag Giu gct cct ggt gag Ala Pro Gly Glu 385 gtg atg ctg ggc Vai Met Leu Gly 370 acc atc acc atc Thr Ile Thr Ile tcc atg ggc gct Ser Met Giy Aia 405 tac tcc aag gac Tyr Ser Lys Asp ggc cgt gga Giy Arg Gly 410 ttc cag tct Phe Gin Ser 425 gaa ggt cgc Giu Gly Arg 395 ctt Leu gac Asp cgt tac cgc ggc atg Arg Tyr Arg Gly Met 400 agt ggt gag aag cgt Ser Gly Glu Lys Arg 415 ggc Giy tcc Ser 420 gtt aag agc Vai Lys Ser 430 gtg cct ttc cgc Val Pro Phe Arg 445 gga ctt cgt gca Giy Leu Arg Ala gaa gac aag ctc gtt cca gaa Glu Asp Lys Leu Val Pro Giu 435 ggt ccc atc gga gac atc att Gly Pro Ile Gly Asp Ile Ile 450 gca atg ggc tac acc ggt tcc Aia Met Giy Tyr Thr Gly Ser cac cag cag 1507 His Gin Gin 455 tcc acc att 1555 Ser Thr Ile 470 gcg ggt ctg 1603 Ala Gly Leu gtc ggt Vai Giy 460 465 gaa gag ctg Giu Giu Leu 475 aag gaa tcc Lys Giu Ser cac aac gct cgt ttc His Asn Ala Arg Phe 480 cac ccg cac cac atc His Pro His His Ile gtg cag atc acc agc Val Gin Ile Thr Ser 485 cag cag act gtg gaa Gin Gin Thr Val Giu gct cct 1641 Ala Pro aac tac Asn Tyr 505 490 cac His tagattttgc tcacttaaac agc 1295 WO 01/00843 WO 0100843PCTIIBOO/00923 <210> 946 <211> 506 <212> PRT <213> Corynebacteriun giutanicuin <400> 946 Met Thr Thr Gin Ser Arg Val Ser Thr Giy Gly Asp Asn Pro Asn Lys 1 5 10 Val Ala Leu Val Gly Leu Thr Phe Asp Asp Val Leu Leu Leu Pro Asp 25 Ala Ser Asp Val Val Pro Ser Giu Val Asp Thr Ser Thr Gin Leu Thr 40 Arg Asn Ile Arg Leu Asn Thr Pro Ile Leu Ser Ala Ala Met Asp Thr 55 Vai Thr Giu Ala Arg Met Ala Ile Giy Met Ala Arg His Gly Gly Ile 70 75 Giy Val Leu His Arg Asn Leu Ser Ilie Gin Giu Gin Ala Giu Asn Val 90 Giu Leu Val Lys Arg Ser Glu Ser Gly Met Val Thr Asp Pro Val Thr 100 105 110 Cys Thr Pro Asp Met Ser Ile Gin Giu Val Asp Asp Leu Cys Ala Arg 115 120 125 Phe Arg Ile Ser Giy Leu Pro Val Val Asp Giu Ala Gly Lys Leu Val 130 135 140 Gly Ile Cys Thr Asn Arg Asp Met Arg Phe Giu Ser Asp Met Asn Arg 145 150 155 160 Arg Vai Ala Giu Val Met Thr Pro Met Pro Leu Val Vai Ala Giu Giu 165 170 175 Gly Val Thr Lys Giu Gin Ala Leu Ala Leu Leu Ser Ala Asn Lys Val 180 185 190 Glu Lys Leu Pro Ile Ile Ala Lys Asp Gly Lys Leu Val Gly Leu Ile 195 200 205 Thr Val Lys Asp Phe Val Lys Thr Giu Gin His Pro Asn Ala Ser Lys 210 215 220 Asp Ala Ser Gly Arg Leu Leu Val Ala Ala Gly Ile Gly Thr Gly Giu 225 230 235 240 Giu Ser Phe Gin Arg Ala Gly Ala Leu Ala Asp Ala Gly Val Asp Ile 245 250 255 Leu Val Val Asp Ser Ala His Ala His Ser Arg Gly Val Leu Asp Met 260 265 270 Val Ser Arg Val Lys Lys Ser Phe Pro Lys Val Asp Ile Val Gly Gly 275 280 285 1296 WO 01/00843 WO 0100843PCTIBOO/00923 Asn Leu Ala Thr Arg Giu Ala Ala Gin Ala Met Ile Giu Ala Gly Ala 290 295 300 Asp 305 Val Ala Met Ser Giu 385 Gly Ser Lys Ilie Gly 465 Vai Gin Lys Gly Ala 340 Ser Leu Thr Gly Lys 420 Pro Ile Giy Thr Vai 500 Val1 Val 325 His Gly Gly Ile Ala 405 Asp Glu Ile Ser Ser 485 Glu Gly 310 Gly Lys Asp Ser Asn 390 Met Arg Gly His Ser 470 Ala Al a Ser 315 Thr Ile Leu Thr

LYS

395 Leu Asp Val1 Giy Leu 475 Ser Ile Ala Ile Ala Ala 380 Arg Ser Val1 P'ro Leu 460 His His Cys Ile Ala Ala 365 Giu Tyr Giy Lys Phe 445 Arg Asn Pro <210> 947 <211> 574 <212> DNA <213> Corynebacterium glutainicum <2 <221> CDS <222> (101)..(574) <223> FRXA00492 <400> 947 gttgtggaat agcgtgtgag ctgcagcggt tatccgattt aaggacaagc tactaaagtt tagataattg tggacacact atgaggagga actgtcgcac atg aca acc cag agc 115 Met Thr Thr Gin Ser 1 1297 WO 01/00843 cga gtt tct acc PCTIBOOIOO923 Arg tta Leu cct Pro aac Asn atg Met aac Asn tct Ser agc Ser c tg Leu Val Ser Thr gga gga Giy Gly gac aac cca aac aag gtt gcc ctt Asp Asn Pro Asn Lys Val Ala Leu 15 ttt gat Phe Asp gag gta Glu Val cct att Pro Ile gta ctt Val Leu acc tcg Thr Ser tct gcc Ser Ala gca cgc Ala Arg 75 gag cag Giu Gin gtc act Val Thr ttg ctt Leu Leu 30 acg cag Thr Gin 45 gca atg Ala Met tcg Ser aat Asn acc Thr gt t Vai ctg Leu act Thr cgc Arg 130 att Ile gac Asp att Ile gag Giu ttg Leu gtg Vali cct Pro 115 att Ile tgc Cys gtt Val gtt Val1 cgc Arg gc t Ala cac His aag Lys 100 gac Asp t cc Ser acc Thr gga Gly gtt Val1 ctt Leu cgc Arg cgc Arg cgt Arg atg Met ggt Gly aac Asn gat Asp gag Glu cgc gat atg cgt ttt gaa agc gac atg Arg Asp Met Arg Phe Glu Ser Asp Met 150 155 <210> 948 <211> 158 <212> PRT <213> Corynebacteriun glutamicun <400> 948 Met Thr Thr Gin Ser Arg Vai Ser Thr 1 5 Vai Aia Leu Val Giy Leu Thr Phe Asp 25 Ala Ser Asp Val Val Pro Ser Glu Val 40 Arg Asn Ile Arg Leu Asn Thr Pro Ile 55 Val Thr Giu Ala Arg Met Ala Ile Gly 70 Asp Asn Pro Asn Lys Leu Leu Leu Pro Asp Ser Thr Gin Leu Thr Ala Ala Met ASP Thr Arg His Gly Giy Ile 1298 WO 01/00843 WO 0100843PCTIBOOIOO923 Gly Val Leu His Arg Asn Leu Ser Ile Gin 90 Glu Leu Val Lys Arg Ser Giu Ser Giy Met 100 105 Cys Thr Pro Asp Met Ser Ilie Gin Giu Val 115 120 Phe Arg Ile Ser Giy Leu Pro Vai Vai Asp 130 135 Giy Ilie Cys Thr Asn Arg Asp Met Arg Phe 145 150 <210> 949 <211> 557 <212> DNA <213> Corynebacterium giutainicum Gin Aia Glu Asn Vai Thr Asp Pro Val Thr 110 Asp Leu Cys Ala Arg 125 Ala Giy Lys Leu Val 140 Ser Asp Met <220> <22i> CDS <222> (534) <223> FRXA00488 <400> 949 cag atc act gcg atc Gin Ilie Thr Ala Ile 1 gtt cct atc atc gcc Val Pro Ilie Ilie Ala aag gct ttg gct gct Lys Ala Leu Ala Ala gct ggt acc gct gag Ala Gly Thr Ala Giu cag tac aag cgt tac Gin Tyr Lys Arg Tyr cgt gga ctt agt ggt Arg Gly Leu Ser Gly cag tct gac gtt aag Gin Ser Asp Vai Lys 100 ggt cgc gtg cct ttc Gly Arg Val Pro Phe 115 atg gag Met Glu gat ggc Asp Gly ggc gct Giy Ala gct cct Ala Pro 55 cgc ggc Arg Gly gag aag Glu Lys agc gaa Ser Giu cgc ggt Arg Gly gc t Ala tct Ser ctg Leu acc Thr ggc Gly aag Lys cca Pro atc Ile cac His ggt Gly ggc Gly atc Ile gc t Al a gac Asp gaa Glu at t Ile 125 48 96 144 192 240 288 336 384 432 gtc ggt gga ctt cgt gca gca atg ggc tac acc ggt tcc tcc acc att 1299 WO 01/00843 PCTIiBOO/00923 Vai Gly Gly Leu Arg Ala Ala Met Gly Tyr Thr Gly Ser Ser Thr Ile 130 135 140 gaa gag ctg cac aac gct cgt ttc gtg cag atc acc agc gcg ggt ctg 480 Giu Giu Leu His Asn Ala Arg Phe Vai Gin Ile Thr Ser Ala Gly Leu 145 150 155 160 aag gaa tcc cac ccg cac cac atc cag cag act gtg gaa gct cct aac 528 Lys Giu Ser His Pro His His Ile Gin Gin Thr Vai Giu Ala Pro Asn 165 170 175 tac cac tagattttgc tcacttaaac agc 557 Tyr His <210> 950 <211> 178 <212> PRT <213> Corynebacteriun giutamicum <400> 950 Gin Ilie Thr Ala Ile Met Giu Ala Ala Val Pro Ala His Lys Ala Gly Ile Leu Thr Lys Leu Asp Val1 115 Gly Leu Ile Ala Ala Arg Ser Val 100 Pro Leu His Asp Gly Ala Arg Giu Ser Arg Ala Ala 150 Met Gin Ser Val Glu Thr Gly Ser Ser Tyr Lys Leu 105 Ile Gly Gly Tyr Val Gin Ser Leu Thr Giy Lys Pro Ile Gly 140 Thr Gly Gly Ile Ala Asp Glu Ile 125 Ser Ser Lys Giu Ser His Pro His His Ile Gin Gin Thr Val Giu Ala Pro Asn Tyr His <210> 951 <211> 1554 1300 WO 01/00843 WO 0100843PCTIiBOO/00923 <212> DNA <213> Corynebacterium giutanicum <220> <221> CDS <222> (101)..(1531) <223> RXA02469 <400>. 951 cgtcgaaaag cagtagtaaa gcagcaggaa ggtggtggaa agagcaattg aggtaccctt gccgtgttca gggataactg attaatcaac taggagaccc atg Met 1 cgt ttt ctt Arg Phe Leu aat tcc aat ccg ccc tat gag ctg aca Asri Ser Asn Pro Pro Tyr Glu Leu Thr tat 15 tcc gat gtg ttt Ser Asp Val Phe atg gtg Met Val cct tcg cgt Pro Ser Arg aat gac gga Asn Asp Gly gac gta ggc tcc Asp Val Gly Ser atg tcc gtg gat ctg cgc acc Met Ser Val Asp Leu Arg Thr act gga aca acc Thr Gly Thr Thr ccg ctc gtt gtg Pro Leu Val Val aac atg act Asn Met Thr gct gtt Ala Val gct ggc cga cgc Ala Gly Arg Arg gct gag acc att Ala Glu Thr Ile cgc cgc ggt ggc Arg Arg Gly Gly atg Met gcg att ctt ccg Ala Ile Leu Pro gat gtg ccc gcc Asp Val Pro Ala gat Asp att gct gca gag Ile Ala Ala Giu atc gcg aat gtg Ile Ala Asri Val aaa gcg gac ctg Lys Ala Asp Leu ttt gat acc cca att acc Phe Asp Thr Pro Ile Thr 100 gta aaa ccg Val Lys Pro cgt gcg cac Arg Ala His 120 cac His 105 cac acc gtg ggt His Thr Val Gly gca cgc aac ttg Ala Arg Asn Leu atc cac aag Ile His Lys 115 ccg gtc ggc Pro Val Gly ggt gca gcc att Gly Ala Ala Ile gtt Val1 125 ttg gag ggc gat Leu Giu Gly Asp cag Gin 130 atc gtc Ile Val 135 ace gac aag gac Thr Asp Lys Asp ctc Leu 140 gaa ggc gca gac Giu Gly Ala Asp aac Asn 145 ttc act cag gtg Phe Thr Gin Val ggc Gly 150 acc ctc atg tcc Thr Leu Met Ser ac Thr 155 tcc cta ttg acg Ser Leu Leu Thr cct gag gat att Pro Glu Asp Ile tcc Ser 165 ccc gaa gac gcc Pro Glu Asp Ala ttc Phe 170 gga atc ctc cac Gly Ile Leu His cac agc cgc aaa His Ser Arg Lys ctc gcC Leu Ala 180 ccc gtc gtc gcg gct gac ggc tca cte cgc ggc atc ctc acc cgc ace Pro Val Val Ala Ala Asp Gly Ser Leu Arg Gly Ile Leu Thr Arg Thr 1301 WO 01/00843 WO 0100843PCT/IBOO/00923 ggc gcc ctg Gly Ala Leu 200 cgc ctg cga Arg Leu Arg gcc acc atg Ala Thr Met ccg gct atc Pro Ala Ile 195 gcc aac ggc Ala Asn Gly atc gaa gga Ile Giu Gly gtc ggc gcc Val Gly Ala ggc ate aac Gly Ile Asn 215 cgc acc Arg Thr aaa acg ctt Lys Thr Leu 230 aca Thr ctc Leu 235 gcc ggc gcc Ala Gly Ala gac Asp 240 agc Ser cta gtc gte Leu Val Val gac Asp 245 gca cac ggc Ala His Gly cgc gca etc Arg Ala Leu gcc gat ggt Ala Asp Gly 280 gta ggc gtt 1027 Val Gly Val 295 gtt ggc cga 1075 Val Gly Arg 310 cgc aaa etc 1123 Arg Lys Leu gac Asp 265 gtc Val1 cac caa tee His Gin Ser 250 gte aac gtc Vai Asn Vai ege gac eta Arg Asp Leu ace atg Thr Met ccc ate Pro Ile 270 ate Ile 255 gt t Vali gee etc aaa Aia Leu Lys ege ate Arg Ile 260 get ggc aac Ala Gly Asn gtg gte ace Val Val Thr 275 gga cca ggc gca Gly Pro Giy Ala 300 eca cag tte tee Pro Gin Phe Ser gtt Val 285 atg Met gca Ala gaa gca ggc gca Giu Ala Giy Ala tgc ace ace ege Cys Thr Thr Arg 305 gtg etg gaa tge Val Leu Glu Cys aac ate ate aag Asn Ile Ile Lys 290 atg eaa ace gge Met Gin Thr Gly gca geec gaa gee Ala Ala Giu Ala 325 gte cgc gac ccc Val Arg Asp Pro 340 ggc gea Gly Ala 330 gee etg Ala Leu 315 cac His gca Ala 320 gta tgg gca Val Trp Ala etc gca get Leu Ala Ala gae Asp 335 ggc gga Gly Gly ege gac 1171 Arg Asp gga tee 1219 Gly Ser gaa tee 1267 Giu Ser 375 ege gee 1315 Arg Ala 390 gte Val1 345 tgg tte Tro Phe 350 tee gga ace tac Ser Gly Thr Tyr 365 gaa Glu ggc gee tee aae gtg atg gte Gly Ala Ser Asn Val Met Val 355 tee ccc gge gac etc ege tte Ser Pro Gly Asp Leu Arg Phe 370 tee tte ggt atg gca tee egg Ser Phe Gly Met Ala Ser Arg gga ege atg tac Gly Arg Met Tyr 380 gaa age ega aae Glu Ser Arg Asn aaa gaa LIys Giu gtg Val1 385 caa aag gte gaa get Gin Lys Val Glu Ala 400 ttc gaa aaa gca Phe Glu Lys Ala 405 395 1302 WO 01/00843 cgc cgc gca atg ttc gaa gaa ggc 1363 Arg Arg Ala Met Phe Giu Giu Gly 410 gac aaa cgc cac ggc gga grc gag 1411 Asp Lys Arg His Gly Gly Val Glu 425 ggt grc cgc tcc tca ttc acc tac 1459 Gly Val Arg Ser Ser Phe Thr Tyr 440 445 ttc ttc gaa cgc gcc acc gtc gga 1507 Phe Phe Glu Arg Ala Thr Vai Gly 455 460 gaa gga aag cca cgc gct tcg cgr 1554 Glu Gly Lys Pro Arg Ala Ser Arg 470 475 <210> 952 <211> 477 <212> PRT <213> Corynebacteriun giutanicui PCTIIBOO/00923 atc rcc act gcc cgc arc rac att Ile Ser Thr Ala Arg Ile Tyr Ile 415 420 gac crg gta gat caa atc arc rcc Asp Leu Val Asp Gin Ile Ile Ser 430 435 gca ggc gcc gat rcg art gaa acc Ala Gly Ala Asp Ser Ile Glu Thr 450 gtt caa tcc acc gaa ggc tac gca Vai Gin Ser Thr Giu Giy Tyr Ala 465 taagtgttgt tgtgcctttg aga <400> 952 Met Arg Phe Leu Asn Asn Ser Asn Pro 1 Asp Val1 Val1 Ala Ile Asp Asn Asp Asn 145 Phe Leu Asn Arg Ala Pro Ile 115 Pro Thr Arg Gly 40 Ala Ile Asn Pro His 120 Thr Leu Ser Thr Gly Leu Val His 105 Gly Asp Met Pro Asp Gly Arg Pro Lys His Ala Lys Ser Tyr Val Thr Arg Gin Lys Thr Ala Asp Thr 155 Giu Gly Thr Met Asp Ala Val1 Ile Leu 140 Ser Leu Ser Ile Ala Val Asp Gly Val 125 Giu Leu 1303 WO 01/00843 WO 0100843PCTIBOO/00923 Pro Ser Ile Ile Giy 225 Val Ala Gly Ala Arg 305 Cys Gly S er Gly Gly 385 Ala Ala Asp Asp Thr 465 Pro Pro Giy Arg Arg 230 Thr Arg Ala Val1 Val1 310 Arg Arg Gly Glu Arg 390 Arg Asp Gly Phe Giu 470 Glu Val1 Ala Leu 215 Thr Ala Ala Asp Gly 295 Gly Lys Asp Ser Ser 375 Ala Arg Lys Val1 Phe 455 Gly Asp Val1 Leu 200 Arg Lys His Leu Gly 280 Val1 Arg Leu Val1 Trp, 360 Asp Val1 Ala Arg Arg 440 Glu Lys Phe Gly 170 Ala Asp Ala Thr Gly Ala Leu Leu 235 His Gin 250 Val Asn Arg Asp Pro Gly Gin Phe 315 Ala His 330 Leu -Ala Ser Gly Arg Met Ser Arg 395 Phe Giu 410 Gly Gly Ser Phe Ala Thr Arg Ala 475 Leu Ser Tyr 205 Ile Ala Thr Pro Val1 285 Met Ala Trp Ala Tyr 365 Lys Gin Gly Giu Tyr 445 Gly Arg His Leu 190 Lys Gly Gly Met Ile 270 Giu Cys Val1 Ala Ala 350 Giu Giu Lys Ile Asp 430 Al~a Val1 Glu 175 Arg Pro Ile Ala Ile 255 Val1 Ala Thr Leu Asp 335 Gly Ser Ser Val Ser 415 Leu Gly Gin 1304 WO 01/00843, WO 01/0843'PCTIIBOO/00923 <210> <211> <212> <213> <220> <221> <222> <223> 953 1692

DNA

Corynebacterium

CDS

(101)..(1669) RXN00487 giutamicuin <400> 953 agggcgccag gggcatccag ccattaaagc ttttcgacga gccctcgccc atgtggccaa agaatcttat ttggaggctc gtctagtaga gtgagttctt gtg agc ctt cag Val Ser Leu Gin aat cat cgc cca Asn His Arg Pro ctc gtc gtt gac Leu Val Val Asp ttc Phe ggc gca cag tac Gly Ala Gin Tyr gcg cag Ala Gin ctg atc gca cgt cgt gtg cgt gag gcc ggc atc tac tcc gaa gtc atc Leu Ile Ala Arg Arg Val Arg Glu Ala Gly Ile Tyr Ser Glu Val Ile ccg cac acc Pro His Thr gcc acc gca gac gat gtg cgc gct aaa aat gca gca gcc Ala Thr Ala Asp Asp Val Arg Ala Lys Asn Ala Ala Ala ctc gtc Leu Val ctt tcc ggt ggc Leu Ser Gly Gly tcc tcc gtg tat Ser Ser Val Tyr gag gga gca cca Giu Gly Ala Pro ctt gac gct gag Leu Asp Ala Giu ctt gat ctc gga Leu Asp Leu Gly t tg Leu cca gta ttt ggc Pro Val Phe Gly tgc tac ggc ttc Cys Tyr Gly Phe gcc atg acc cac Ala Met Thr His ctt ggt ggc acc Leu Gly Gly Thr gtt gcc Val Ala 100 aac acc ggt Asn Thr Gly ggc gtc ctc Gly Val Leu 120 aag Lys 105 cgc gaa tac gga Arg Giu Tyr Gly acc gac atc aac gtt gcc ggt Thr Asp Ile Asn Val Ala Gly 115 cac gaa ggc Ctc His Giu Gly Leu gcc tgc cac aag Ala Cys His Lys gtg Val1 130 tgg atg agc Trp Met Ser cac ggc His Gly 135 gac gcc gtc tct Asp Ala Val Ser gcc cca gaa ggt Ala Pro Giu Gly t tc Phe 145 gta gtc acc gcg Val Val Thr Ala tcc gaa ggt gcg Ser Giu Gly Ala gtc gca gct ttc Val Ala Ala Phe gaa Glu 160 aac aag gaa cgc Asn Lys Giu Arg atg gct ggc gtg Met Ala Gly Val tac cac cca gag Tyr His Pro Glu ttg cac tca cca Leu His Ser Pro cac ggc His Gly 180 1305 WO 01/00843 WO 0100843PCTIIBOOI00923 cag gca Gin Ala aac tgg Asn Trp gag cag Glu Gin 215 gtg gac Val Asp 230 cgt ttg Arg Leu cgc gag Arg Giu gtt acc Val- Thr acc gaa 1027 Thr Giu 295 tcc ttc 1075 Ser Phe 310 tcc acc 1123 Ser Thr gaa tcc 1171 Giu Ser gtc ggt 1219 Val Gly cgt gac 1267 Arg Asp 375 ctg cct 1315 gtt ctg Val Leu 185 acc gca Thr Ala 200 atc ggc Ilie Gly tcc gct Ser Ala acc cgc Thr Arg gca aac Ala Asn gaa gat Giu Asp gtt gcc Vai Ala 235 gtc ttt Val Phe 250 gaa aaa Giu Lys gag cgc Giu Arg gca aag Ala Lys gca gtt Ala Val 315 ttc ctg Phe Leu 330 gga tct Gly Ser cca gac Pro Asp aaa gac Lys Asp atc gtt ttc Phe atc Ile Gly 220 ggt Gly gt t Val1 gac Asp cag Gin cgc Arg 300 gcc Ala gtt Val1 ggt Gly gat Asp gaa Giu 380 ggc C tC Leu gct Ala 205 c gc Arg get Ala gac Asp ttc Phe gca Ala 285 aag Lys gg t Gly cag Gin acc Thr gtg Val 365 gtc Val1 cgc gag atc gca ggt Giu Ile Ala Giy gaa etc atc gaa Giu Leu Ile Giu 210 att tgt ggc eta Ile Cys Gly Leu 225 gtg cag cgc gcc Val Gin Arg Ala 240 ggt ctg ctg cgt Gly Leu Leu Arg 255 gca gca ace ggc Ala Ala Thr Gly cta tcc aag ctg Leu Ser Lys Leu 290 atc ggc get gag Ile Giy Ala Giu 305 ctg gaa gaa gct Leu Giu Giu Ala 320 ace ctg tac cca Thr Leu Tyr Pro 335 aac atc aag age Asn Ile Lys Ser ttc aag ctt gtt Phe Lys Leu Vai 370 gcc gtt ggc egt Ala Val Gly Arg 385 cca ttc cca gga, cta Leu 195 aag Lys tee Ser att Ile gc Ala gcc Ala 275 gcc Ala ttc Phe cca Pro gac Asp cac His 355 gag Giu gaa Glu cca gag Giu gte Val1 ggt Gly ggt Giy ggt Giy 260 aag Lys gga Gly atc Ile gaa Giu gte Vai 340 c ac His cca Pro ctt Leu gga cgc Arg gac Asp ggt Gly 345 ctg Leu ttc Phe gaa 1306 WO 01/00843 WO 0100843PCTIBOO/00923 Leu Pro 390 ggt atc 1363 Gly Ile cgc cac 1411 Arg His gac ggc 1459 Asp Giy tct gtt 1507 Ser Val 455 ctg cgc 1555 Leu Arg 470 ctg cca 1603 Leu Pro gtt cca 1651 Val Pro gga acc 1692 Gly Thr Giu Glu Ile cgc atc atc Arg Ile Ile 410 Val 395 ggt Gly Gly Arg Gin Pro gaa gtc ace gaa Giu Val Thr Giu 415 Pro Gly Pro Gly gat cgc cta gaa acc etc Asp Arg Leu Giu Thr Leu 420 ctc acc gaa gcc gga ctt Leu Thr Giu Ala Gly Leu 435 get gac Ala Asp 425 gtg atc Val Ile ctg atc gcc cgc acc Leu Ilie Ala Arg Thr 430 tgg cag tgc cca gta Trp Gin Cys Pro Val 445 gag Giu gte etc ctg gca gat gte cgc Val Leu Leu Ala Asp Val Arg 450 gtt caa ggc Val Gin Gly gat ggc cgc ace tac gga cac cca atc gtg Asp Gly Arg Thr Tyr Giy His Pro Ile Vai 460 465 gaa gac gca atg acc gcc gac tgg acc cgc Glu Asp Ala Met Thr Ala Asp Trp Thr Arg cca gtg tct tc Pro Val Ser Ser 475 tac gag gtt ctg Tyr Giu Val Leu 490 gat gtg aac cgc Asp Val Asn Arg 505 atc gaa tgg gag Ilie Giu Trp, Giu 520 gag aag atc tee Glu Lys Ile Ser 495 gtg gtg ctg gac Val Val Leu Asp acc cgc atc ace aac gaa Thr Arg Ile Thr Asn Giu 500 gta ace tcc aag cca cca Val Thr Ser Lys Pro Pro taggccttaa atgagccttc gtt <210> 954 <211> 523 <212> PRT <213> Corynebacteriui giutainicum <400> 954 Vai Ser Leu Gin Thr Asn His Arg Pro 1 5 Ala Gin Tyr Ala Gin Leu Ile Aia Arg 25 Tyr Ser Giu Val Ilie Pro His Thr Ala 40 Lys Asn Aia Aia Ala Leu Val Leu Ser 55 Val Leu Val Val Asp Phe Gly 10 Arg Vai Arg Giu Ala Giy Ilie Thr Ala Asp Asp Val Arg Ala Gly Gly Pro Ser Ser Val Tyr 1307 WO 01/00843 WO 0100843PCT/iBOO/00923 Al a Pro Gly Ile Lys Phe 145 Asn His Ala Ile Gly 225 Arg Leu Thr Lys Ala 305 Giu Tyr Lys Leu Giu Val Gly Asn Val 130 Val1 Lys Ser Gly Giu 210 Leu Ala Arg Gly Leu 290 Glu Ala Pro Ser Val1 370 Gly Phe Thr Val1 115 Trp Val1 Giu Pro Leu 195 Lys Ser Ile Ala Ala 275 Ala Phe Pro Asp His 355 Glu Pro Ile Ala Gly Ser Ala Lys 165 Gly Gin Arg Gly Asp 245 Giu Leu Val1 Arg Gly 325 Val Asn Leu Ala Phe Lys 105 His Ala Gly Val1 Leu 185 Ala Gly Ala Cys Val1 265 Asp Glu Arg Asp Gly 345 Leu Phe Giu Gin 90 Arg Giu Val1 Ala Gin 170 Thr Ala Glu Val1 Val1 250 Glu Glu Ala Ala Phe 330 Gly Pro Lys Ile 75 Ala Giu Gly Ser Pro 155 Tyr Arg Asn Asp Ala 235 Phe Lys Arg Lys Val 315 Leu Ser Asp Asp Leu Met Tyr Leu Giu 140 Val1 His Phe Ile Gly 220 Gly Val Asp Gin Arg 300 Ala Val Gly Asp Giu 380 Gly Ala Thr Cys Glu Phe Val1 175 Giu Giu Ile Val1 Gly 255 Ala Leu Ile Leu Thr 335 Asn Phe Ala Leu Leu Asp His Gly Glu 160 Leu Ile Leu Cys Gin 240 Leu Ala Ser Gly Giu 320 Leu Ile Lys Val1 1308 WO 01/00843 Gly Arg Gl 385 Pro Gly Pr Arg Leu Gi Thr Glu Al 43 Leu Ala As 450 Gly His Pr 465 Ala Asp Tz Arg Ile Thb Thr Ser Ly Gly Leu 405 Leu Leu Arg Val1 Arg 485 Glu Pro Leu 390 Gly Arg Asp S er Leu 470 Leu Val Gly Gly Glu Ala Cys Asp 460 Glu Glu Val1 Arg Val Arg Pro 445 Gly Asp Lys Val Gln Thr Thr 430 Val Arg Al a Ile Leu 510 PCTJIBOO/00923 Pro Phe 400 Glu Asp 415 Glu Leu Val Leu Thr Tyr Met Thr 480 Ser Thr 495 Asp Val <210> 955 <211> 1486 <212> DNA <213> Corynebacteriun glutanicum <220> <221> CDS <222> (101)..(1486) <223> FRXA00487 <400> 955 agggcgccag gggcatccag ccattaaagc ttttcgacga agaatcttat ttggaggctc gtctagtaga gtgagttctt gccctcgccc atgtggccaa gtg agc ctt cag aca Val Ser Leu Gln Thr aat Asn c tg Leu ccg Pro C tC Leu gta Val1 cgt Arg acc Thr ggt Gly ctc Leu gtg Val1 gca Ala ggc Gly tac Tyr gaa Glu gca Ala gga Gly 115 163 211 259 307 1309 WO 01/00843 WO 0100843PCT/IBOO/00923 ctt gac gct gag Leu Asp Ala Giu ctt gat ctc gga Leu Asp Leu Gly cca gta ttt ggc Pro Val Phe Gly att Ile tgc tac ggc ttc Cys Tyr Gly Phe gcc atg acc cac Ala Met Thr His gcg Ala ctt ggt ggc acc Leu Gly Giy Thr gtt gcc Val Ala 100 aac acc ggt Asn Thr Gly ggc gtc ctc Gly Val Leu 120 aag Lys 105 cgc gaa tac gga Arg Giu Tyr Gly acc gac atc aac Thr Asp Ile Asn gtt gcc ggt Val Ala Gly 115 cac gaa ggc ctc His Giu Giy Leu gcc tgc cac aag gtg tgg atg agc Ala Cys His Lys Val Trp Met Ser 130 cac ggc His Giy 135 gac gcc gtc tct Asp Ala Val Ser gcc cca gaa ggt Ala Pro Giu Gly gta gtc acc gcg Val Val Thr Ala 499 547 595 643 tcc gaa ggt gcg Ser Glu Gly Ala gtc gca gct ttc Val Ala Ala Phe gaa Glu 160 aac aag gaa cgc Asn Lys Glu Arg atg gct ggc gtg met Ala Gly Val tac cac cca gag Tyr His Pro Glu ttg cac tca cca Leu His Ser Pro cac ggc His Gly 180 cag gca gtt Gin Ala Val aac tgg acc Asn Trp Thr 200 ctg Leu 185 acc cgc ttc ctc Thr Arg Phe Leu gag atc gca ggt Glu Ile Ala Gly cta gag cag Leu Glu Gin 195 aag gtc cgc Lys Val Arg gca gca aac atc Ala Ala Asn Ile gaa gaa ctc atc Glu Glu Leu Ile gaa Glu 210 gag cag Giu Gin 215 atc ggc gaa gat Ile Gly Glu Asp cgc gct att tgt Arg Ala Ile Cys ggc Gly 225 cta tcc ggt ggt Leu Ser Gly Gly gtg Val 230 gac tcc gct gtt Asp Ser Ala Val ggt gct ttg gtg Gly Ala Leu Val cgc gcc att ggt Arg Ala Ile Gly cgt ttg acc tgt Arg Leu Thr Cys gtc Val1 250 ttt gtt gac cac Phe Val Asp His ctg ctg cgt gcc Leu Leu Arg Ala ggt gag Gly Giu 260 cgc gag cag Arg Giu Gin gtt acc gtt Val Thr Val 280 gaa aaa gac ttc Giu Lys Asp Phe gca gca acc ggc Ala Ala Thr Gly gcc aag ctg Ala Lys Leu 275 gcc gga gtt Ala Gly Val 931 979 gat gag cgc cag Asp Giu Arg Gln ttc cta tcc aag Phe Leu Ser Lys ctg Leu 290 acc gaa cca gaa gca aag cgc aag gct atc ggc gct gag ttc atc cgc 1027 Thr Glu 295 Pro Giu Ala Lys Lys Ala Ie Gly Ala 305 Glu Phe Ile Arg 1310 WO 01/00843 WO 0100843PCT/BOOIOO923 tcc ttc 1075 Ser Phe 310 tcc acc 1123 Ser Thr gaa. tcc 1171 Giu Ser gtc ggt 1219 Val Gly cgt gac 1267 Arg Asp 375 ctg cct 1315 Leu Pro 390 ggt atc 1363 Gly Ile cgc cac 1411 Arg His gac ggc 1459 Asp Gly tct gtt 1486 Ser Val 455 gag cgc gca gt Glu Arg Ala Val 315 gtg gac ttc ctg Val Asp Phe Leu gcc ggt gtg ctg gaa Ala Gly Val Leu Giu 320 gtt cag ggc acc ctg Val Gin Giy Thr Leu gaa gct cca gaa ggt Giu Ala Pro Giu Giy 325 tac cca gac gtc gtg Tyr Pro Asp Val Vai 340 aag agc cac cac aac Lys Ser His His Asn ggt ggt Gly Gly 345 gga ctg Glv Leu 330 gga tct ggt acc gca Gly Ser Gly Thr Ala 350 cca gac gat gtg gaa Pro Asp Asp Val Giu aac atc Asn Ile 355 ttc aag Phe Lys ctc ttc aaa gac gaa Leu Phe Lys Asp Giu 380 gag gaa atc gtt ggc Giu Giu Ile Val Gly 365 gtc Val cgt gcc gtt Arg Ala Val cgc cag cca ttc Arg Gin Pro Phe 400 gtc acc gaa gat Val Thr Giu Asp ctt gtt gag cca cig Leu Val Giu Pro Leu 370 ggc. cgt gaa ctt ggc Gly Arg Glu Leu Gly 385 cca gga cca gga ctt Pro Gly Pro Gly Leu 405 cgc cta gaa acc ctc Arg Leu Giu Thr Leu 420 acc gaa gcc gga ctt Thr Glu Ala Gly Leu cgc atc Arg Ile gct gac Ala Asp 425 gtg atc Val Ile atc Ile 410 gaa Giu 415 ctg atc gcc cgc acc Leu Ile Ala Arg Thr 430 tgg cag tgc cca gta Trp Gin Cys Pro Val gag ctc Glu Leu 435 gtc ctc ctg gca gat gtc cgc Val Leu Leu Ala ASP Vai Arg 450 gtt caa ggc gat Vai Gin Gly Asp 460 445 ggc cgC Gly Arg <210> 956 <211> 462 <212> PRT <213> Corynebacterium glutamicun <400> 956 Val Ser Leu Gin Thr Asn His Arg Pro Val Leu Val Vai Asp Phe Gly 1 5 10 Ala Gin Tyr Aia Gin Leu Ile Ala Arg Arg Val Arg Giu Ala Gly Ile WO 01/00843 WO 0100843PCT/EBOO/00923 25 Tyr Ser Glu Val Ile Pro His Thr Ala Thr Ala Asp Asp Val Arg Ala 40 Lys Asn Ala Ala Ala Leu Val Leu Ser Gly Gly Pro Ser Ser Val Tyr 55 Ala Giu Gly Ala Pro Ser Leu Asp Ala Giu Ile Leu Asp Leu Gly Leu 70 75 Pro Val Phe Gly Ile Cys Tyr Gly Phe Gin Ala Met Thr His Ala Leu 90 Gly Gly Thr Val Ala Asn Thr Gly Lys Arg Giu Tyr Giy Arg Thr Asp 100 105 110 Ile Asn Val Ala Gly Gly Val Leu His Giu Gly Leu Giu Ala Cys His 115 120 125 Lys Val Trp Met Ser His Gly Asp Ala Val Ser Giu Ala Pro Giu Gly 130 135 140 Phe Val Val Thr Ala Ser Ser Giu Gly Ala Pro Val Ala Ala Phe Glu 145 150 155 160 Asn Lys Giu Arg Lys Met Ala Gly Val Gin Tyr His Pro Giu Val Leu 165 170 175 His Ser Pro His Gly Gin Ala Val Leu Thr Arg Phe Leu Thr Giu Ile 180 185 190 Ala Gly Leu Giu Gin Asn Trp Thr Ala Ala Asn Ile Ala Giu Giu Leu 195 200 205 Ile Giu Lys Val Arg Giu Gin Ile Gly Giu Asp Gly Arg Ala Ile Cys 210 215 220 Gly Leu Ser Gly Gly Val Asp Ser Ala Val Ala Gly Ala Leu Val Gin 225 230 235 240 Arg Ala Ile Gly Asp Arg Leu Thr Cys Val Phe Val Asp His Gly Leu 245 250 255 Leu Arg Ala Gly Glu Arg Giu Gin Val Glu Lys Asp Phe Val Ala Ala 260 265 270 Thr Gly Ala Lys Leu Val Thr Val Asp Giu Arg Gin Ala Phe Leu Ser 275 280 285 Lys Leu Ala Gly Val Thr Giu Pro Giu Ala Lys Arg Lys Ala Ile Gly 290 295 300 Ala Glu Phe Ile Arg Ser Phe Giu Arg Ala Val Ala Gly Val Leu Giu 305 310 315 320 Giu Ala Pro Giu Gly Ser Thr Val Asp Phe Leu Val Gin Gly Thr Leu 325 330 335 Tyr Pro Asp Val Val Glu Ser Gly Gly Gly Ser Gly Thr Ala Asn Ile 340 345 350 1312 WO 01/00843 WO 0100843PCT/iBOO/00923 Lys Ser His His Asn Vai Gly Gly Leu Pro Asp 355 360 Leu Val Giu Pro Leu Arg Asp Leu Phe Lys Asp 370 375 Gly Arg Giu Leu Gly Leu Pro Giu Giu Ile Val 385 390 395 Pro Giy Pro Giy Leu Gly Ile Arg Ile Ile Giy 405 410 Arg Leu Giu Thr Leu Arg His Ala Asp Leu Ile 420 425 Thr Giu Ala Gly Leu Asp Gly Val Ile Trp Gin 435 440 Leu Ala Asp Val Arg Ser Val Gly Vai Gin Giy 450 455 <210> 957 <211> 693 <212> DNA <213> Corynebacteriun glutanicun <22 0> <221> CDS <222> (101)..(670) <223> RXA02237 <400> 957 gacgagctgg gcattgctca gacccgtcgt cttcgtggac gcacttctcg agcgtttcgg cttcgaggat taattcttca Phe Ala Pro Glu 415 Glu Val Lys Val1 Phe 400 Asp Leu Leu caa cta gga Gin Leu Giy tcg Ser agt Ser cgt Arg tgt Cys tca cgg Arg gtt Val1 atg Met ttc Phe atg Met cca gcg Ala aat Asn 30 cct Pro cag Gin gat Asp aat 1313 tgggtgaccg tcagcgtcgc gtg tcg ggc gat aac Val Ser Gly Asp Asn 1 tca gcg gtc ggt aaa Ser Ala Val Gly Lys cca aac ctg tat ttc Pro Asn Leu Tyr Phe ggt gaa gtc gat gga Gly Giu Val Asp Gly cag gac aaa atc gac Gin Asp Lys Ile Asp ggc ggt ttg cag cgt Gly Gly Leu Gin Arg cgc caa aat ggt cgg ii 163 211 259 307 355 403 WO 01/00843 Ser Gly Thr Pro Ala Gly Pro Val 1 cca gta ttg gtt gag gtt gat ctt Pro Val Leu Val Glu Val Asp Leu 2 105 tta att cca gat gca gaa acc atc I Leu Ilie Pro Asp Ala Glu Thr Ile 1 120 125 gtt ttg gtt gaa cgc ctc act gga Val Leu Val Giu Arg Leu Thr Gly 1 135 140 att gct cgc agg ctc gag acc gca Ile Ala Arg Arg Leu Giu Thr Ala 2 150 155 gaa ttt aag cac gtc att atc aat( Giu Phe Lys His Val Ile Ile Asn 2 170 get att gag gat gtt etc ctc ggcc Ala Ile Giu Asp Val Leu Leu Gly 2 185 agg <210> 958 <211> 190 <212> PRT <213> Corynebacterium glutamicum <400> 958 Val Ser Gly Asp Asn Gin Leu Giy 1 5 Ser Ala Val Giy Lys Ser Thr Val Pro Asn Leu Tyr Phe Ser Val Ser Gly Glu Val Asp Giy Arg Asp Tyr Gin Asp Lys Ilie Asp Cys Gly Giu Gly Gly Leu Gin Arg Ser Gly Thr Arg Gin Asn Gly Arg Pro Val Leu 100 Arg Asn Ile Ala Ser Leu Ile Pro 115 120 Pro Pro Ser Trp Giu Val Leu Val PCTISBOO/00923 Giu Ala Arg Gin Asn Giy Arg 95 100 gga gcc cga aac atc gct agc 451 Gly Ala Arg Asn Ile Ala Ser 115 ett gct cca cct tca tgg gaa 499 Leu Ala Pro Pro Ser Trp Giu 130 ggc acc gaa agc gaa gac gtt 547 Gly Thr Giu Ser Glu Asp Val 145 gaa gaa ttg gct get cag agc 595 Glu Giu Leu Ala Ala Gin Ser 160 165 gat gtg gat aca gcc gtc aag 643 Asp Val Asp Thr Ala Val Lys 175 180 tagccaaaac atagagcggt 690 krg I lal 2 4et Phe 4et I Pro I ltal 105 k.sp 2 3iu 2 1314 Gly Asp Arg Giu Ile Glu Gly Leu Gly WO 01/00843 PCT(1BO0100923 130 135 140 Glu Ser Glu Asp Val Ile Ala Arg Arg Leu Giu Thr Ala Arg Giu Glu 145 150 155 160 Leu Ala Ala Gin Ser Glu Phe Lys His Val Ile Ile Asn Asp Asp Val 165 170 175 Asp Thr Ala Val Lys Ala Ile Giu Asp Val Leu Leu Giy Ala 180 185 190 <210> 959 <211> 1413 <212> DNA <213> Corynebacteritum glutaiicun <22 0> <221> CDS <222> (101)..(1390) <223> RXA01446 <400> 959 gatgattaaa aacctcacat gggatagtaa gatgaaataa cgtctgtgtg cgcacacgcg tgcatactcc aacttcatgg atcggatgtg acgtaaacca atg gct gca atc gtt Met Ala Ala Ile Val gtc Val ctc Leu gc t Ala ctt Leu ggC Gly gag Giu cac His ttc Phe ggc Gly ggc Gly gga Gly cci: Pro gt Val1 gct Ala ctg Leu c tg Leu 120 gcc ggc gtc Ala Gly Val ggC G ly gtt Val1 ggc Gly acg Thr ctg Leu cgc Arg gtg Val ac c Thr aaa Lys aag Lys gag Glu gcc Ala t tc Phe c tg Leu atg Met acc Thr 1 ggt Gly ccc Pro aag Lys acc Thr gaa Glu cgc Arg gac Asp ggc Gly aag Lys.

aat Asn tac Tyr cca Pro gaa Glu atc Ile cgt Arg cgt Arg 130 gug Val cgc Arg gt t Val 105 ggc Gly 1315 WO 01/00843 cca acc U~ Pro Thr T) 135 att ttc gz Ile Phe A- 150 tac aaa az Tyr Lys Ac get gag gi Ala Glu G)

IC

Ia

-U

Li 1C 0S -v ccc atg Pro Met ggc aag Gly Lys 215 gac cac Asp His 230 ggc gca Gly Ala ttg ggt Leu Gly cca act Pro Thr ggc gag 1027 Gly Glu 295 gac tcc 1075 Asp Ser 310 tac tte 1123 Tyr Phe atc tgc 1171 Ile Cys gt 2( Hi agt Ser atc Ile gag Glu 280 gtc Val1 gtg Val1 ctg Leu gta Val1 gcg Ala gaa Giu cag Gin atc Ile 185 atc Ile gt t Val1 acc Thr gt t Val1 atc Ile 265 ctg Leu ggc Giy att Ile acc Thr gc t Ala 345 gac Asp tcc Ser gtg Val 170 gtg Val1 gat Asp ctt Leu tac Tyr ggt Gly 250 aag Lys ttt Phe gtg Val1 get Ala aag Lys 330 tac Tyr aaa Lys atc Ile 155 ctg Leu cag Gin gcc Ala a tg Met cca Pro 235 tca S er gcc Ala ga t Asp aac Asn cg t Arg 315 eta Leu gac Asp gta Val 140 ctt Leu gtg Val tac Tyr ac c Thr gaa Giu 220 ttc Phe ggt Gly tac aag Lys acc Thr 300 tac Tyr gac Asp gt t Val1 cgc Arg caa Gin atg Met etc Leu 190 gtg Val ggc Gly ac c Thr ggc Gly act Thr 270 9gc Gly cgt Arg tcc Ser ctc Leu ggt Gly 350 gtg Val aaa Lys tac Tyr 175 tcc Ser ctc Leu cag Gin tcc Ser cca Pro 255 cgt Arg gag Giu aag Lys CgC Arg ac Thr 335 gt t gga Gly gtc Val 160 aac Asn t ac Tyr aac Asn gca Ala tc S er 240 ace Thr gtt Val1 tac Tyr cg t Arg gtc Val1 320 ggc Gly cgc atc Ile 145 gaa Glu cgc Arg gc t Ala gag Giu acc Thr 225 aac Asn aag Lys ggt Gly ctg Leu cgc Arg 305 aac Asn atc Ile cac PCTIIBOO/00923 cgt gtt caa gac 547 Arg Val Gin Asp tcc gcc ctg gat. 595 Ser Ala Leu Asp 165 aag gcc atc gtc 643 Lys Ala Ile Val 180 gat cgt etg cgc 691 Asp Arg Leu Arg 195 gca ctt gat cag 739 Ala Leu Asp Gin 210 atg etc gac gtg 787 Met Leu Asp Val cca aCC gcc ggt 835 Pro Thr Ala Gly 245 atc ace agc tcc 883 Ile Thr Ser Ser 260 gcc ggc cea ttc 931 Ala Gly Pro Phe 275 cag acc gtc ggt 979 Gin Thr Vai Giy 290 tgt ggc tgg tac Cys Gly Trp Tyr gga ttc acc gac Gly Phe Thr Asp 325 ggt gaa ate cca Gly Giu Ile Pro 340 gat gaa atg cca Val Arg His Asp Glu Met Pro 355 1316 WO 01/00843 ctg acc cag tca gag ttc cac cac gca 1219 Leu Thr Gin Ser Giu Phe His His Ala 360 365 cct gca tgg gac gaa gac atc acc gac 1267 Pro Ala Trp Asp Giu Asp Ile Thr Asp 375 380 cca caa aag gcc cag gac tac gtc cga 1315 Pro Gin Lys Ala Gin Asp Tyr Val Arg 390 395 gct cgc ttc tcc tac atc ggt gtt gga 1363 Ala Arg Phe Ser Tyr Ile Gly Vai Gly 410 gtc ctg cat gac gta cta gct gac aac 1410 Val Leu His Asp Val Leu Ala Asp Asn 425 430 gag 1413 <210> 960 <211> 430 <212> PRT <213> Corynebacterium glutamicum <400> 960 Met Ala Ala Ile Val Ile Val Gly Ala 1 Gly Lys Ala Thr Asp Ile Leu Gly Gly Pro Asn Gly Gly Asn Asn Ala Gly His 40 Lys Tyr Giu Leu Lys Leu Leu Pro Ala 55 Thr Pro Ile Leu Gly Asn Gly Vai Val 70 Glu Glu Ile Asp Gly Leu Giu Ala Arg Arg Ile Ser Ala Asn Ala His Leu Val 100 105 Asp Arg Val Gin Glu Arg Phe Leu Gly 115 120 Gly Arg Gly Ile Gly Pro Thr Tyr Ala 130 135 PCTIIBOO/00923 acc cca atc ttt gaa acC atg Thr Pro Ile Phe Giu Thr Met 370 tgc aag acc ttc gag gat ctt Cys Lys Thr Phe Glu Asp Leu 385 cgt ctg gaa gaa ctc tct ggt Arg Leu Giu Giu Leu Ser Gly 400 405 cct ggt cgc gat cag acc atc Pro Gly Arg Asp Gin Thr Ile 415 420 tagtactgat aaacatcagt Gly Asp Va1 Leu Leu Asp Tyr Ala Val 140 Asp Tyr Va1 Ser Glu Ala His Ile 125 Ser Lys Lys Glu Ala Phe Leu Met Thr Gly 1317 WO 01/00843 WO 0100843PCTI[BOO/00923 Ile Arg Val Gin Asp Ile Phe Asp Glu Ser Ile Leu Arg Gin Lys Val 145 150 155 160 Glu Ser Ala Leu Asp Tyr Lys Asn Gin Val Leu Val Lys Met Tyr Asn 165 170 175 Arg Lys Ala Ile Val Ala Glu Giu Ile Val Gin Tyr Phe Leu Ser Tyr 180 185 190 Ala Asp Arg Leu Arg Pro Met Val Ile Asp Ala Thr Leu Val Leu Asn 195 200 205 Giu Ala Leu Asp Gin Gly Lys His Val Leu Met Giu Gly Gly Gin Ala 210 215 220 Thr Met Leu Asp Val Asp His Gly Thr Tyr Pro Phe Val Thr Ser Ser 225 230 235 240 Asn Pro Thr Ala Gly Gly Ala Ser Val Gly Ser Gly Ile Gly Pro Thr 245 250 255 Lys Ile Thr Ser Ser Leu Gly Ilie Ile Lys Ala Tyr Thr Thr Arg Val 260 265 270 Gly Ala Gly Pro Phe Pro Thr Giu Leu Phe Asp Lys Trp Gly Glu Tyr 275 280 285 Leu Gin Thr.Vai Gly Gly Giu Val Gly Val Asn Thr Gly Arg Lys Arg 290 295 300 Arg Cys Gly Trp Tyr Asp Ser Val Ile Ala Arg Tyr Ala Ser Arg Val 305 310 315 320 Asn Gly Phe Thr Asp Tyr Phe Leu Thr Lys Leu Asp Val Leu Thr Gly 325 330 335 Ilie Gly Giu Ilie Pro Ile Cys Val Ala Tyr Asp Vai Asp Gly Val Arg 340 345 350 His Asp Giu Met Pro Leu Thr Gin Ser Glu Phe His His Ala Thr Pro 355 360 365 Ile Phe Giu Thr Met Pro Ala Trp Asp Giu Asp Ile Thr Asp Cys Lys 370 375 380 Thr Phe Giu Asp Leu Pro Gin Lys Ala Gin Asp Tyr Val Arg Arg Leu 385 390 395 400 Giu Glu Leu Ser Gly Ala Arg Phe Ser Tyr Ile Gly Val Gly Pro Gly 405 410 415 Arg Asp Gin Thr Ilie Val Leu His Asp Val Leu Ala Asp Asn 420 425 430 <210> 961 <211> 1551 <212> DNA <213> Corynebacteriu giutarnicum 1318 WO 01/00843 <220> <221> CDS <222> (101)..(1528) <223> RXA00619 <400> 961 tcaaaggaag acaccattga aggtgtgcgc aaaatcggag cgactaggtt agtttcggtt cgtggggaat aataatttgc PCTIBOO/00923 aattcatcaa aaaatagcag aag Lys aac Asn atc Ile gag Glu agc Ser atc Ile atg Met cgc Arg atc Ile tcc Ser 150 acc Thr ctc Leu atc Ile ctg Leu gcc Ala gca Ala atc Ile gaa Giu acc Thr tcc Ser gga Gly 135 cac His gca Ala aac Asn gca Ala tgg Trp gtg Val1 at t Ile gcc Ala gaa Giu tcc Ser ctt Leu 120 tcc Ser aac Asn gct Ala cgc Arg aac Asn agt Ser atg Met gaa Glu gat Asp ttc Phe cgc Arg 105 gag Giu cgc Arg gtg Val1 gat Asp tac Tyr 185 gtc Val1 gcc Ala a aa Lys tcc Ser cgt Arg aat Asn gac Asp c tg Leu gca Ala gca Ala gaa Giu 170 cca Pro ctg Leu gaa Giu gcc Ala tat Tyr gag Giu 75 gca Ala cc Leu gtc Val gcg Ala gcg Ala 155 atg Met ctt Leu aac Asn aag Lys aaa Lys 45 gca Ala gtc Val gct Ala gaa Glu aac Asn 125 tac Tyr gca Ala gtg Val1 gga G ly cgt Arg atc Ile 30 gat Asp gtg Val1 ac c Thr ggc Gly aat Asn 110 aag Lys caa Gin act Thr gca Ala atc Ile 190 gcc Ala atg Met gga Gly gac Asp cac His 80 gag Giu gaa Giu att Ile c tg Leu ttg Leu 160 gag Giu ggc Gly g tg Val 1 tcc Ser gag Giu gtt Val cag Gin 65 gat Asp cac His cag Gin gca Ala gtc Val1 145 ggc Gly cgc Arg cca Pro gcg Ala cgc Arg gag Giu 50 gtt Val gtg Val1 atc Ile c tg Leu gtt Val1 130 atg Met aag Lys gtc Val1 atg Met gct gat aaa aag Ala Asp Lys Lys gaa Glu cag Gin 35 atc Ile gat Asp aag Lys cac His cag Gin 115 gtt Val gct Ala cgt Arg acc Thr gga Gly 195 ctt Leu 20 ctc Leu cct Pro ttg Leu gcc Ala aag Lys 100 atc Ile gca Ala ggC Gly ttc Phe gaa Giu 180 acc Thr tct Ser tgg Trp gcc Ala gca Ala cgc Arg ggC Gly cac His gc t Ala cgt Arg gca Ala 165 ctg Leu gcc Ala 115 163 211 259 307 355 403 451 499 547 595 643 691 739 caa gac atg ctt gac ctc atg gaa ggc gac gag gct cgt ctc tcc gat 1319 WO 01/00843 Gin Asp Met Leu Asp 200 PCT11B00100923 Leu Met Giu Gly Asp Glu Ala Arg Leu Ser Asp 205 210 ctg gaa Leu Giu 215 tcc gtc Ser Val 230 got ctg Ala Leu cgt ctc Arg Leu cag gtc Gin Val gag cgc 1027 Giu Arg 295 gtt gct 1075 Vai Ala 310 tcc gtg 1123 Ser Val gga atg 1171 Gly Met cot gcc 1219 Pro Ala act acc 1267 Thr Thr 375 acc gca 1315 Thr Ala 390 atg cgc: 1363 acc Thr ggc Gly gtt Val atg Met gg t Gly 280 gtg Val1 ga t Asp atc Ile tt t Phe atg Met 360 cgt Arg cac His gaa cgc Arg cag Gin cag Gin gcc Ala 265 too Ser ggc Gly Ott Leu cgc Arg gaa Giu 345 a tc Ile atc Ilie gaa Giu aat gca Ala tac Tyr 235 ggc G ly acc Thr gca Ala ctg Leu ggc Gly 315 gtt Val t to Phe cgc Arg atg Met atc Ile 395 ggt cac His cgt Arg ggc Gly act Thr cot Pro 285 gt t Val1 cag Gin ctg Leu aca Thr Ott Leu 365 got Ala gaa Giu cag ggc Gly ctt L eu tca Ser 255 acc Thr aag Lys ct t Leu aac Asn gao Asp 335 ctg Leu cgt Arg cgc Arg gc t Ala Ott Leu ttt Phe gac Asp 240 tcg Ser gaa Giu atg Met cgc Arg gaa Glu 320 gca Ala gat Asp tac Tyr gca Al a gtc Val1 400 atc Ile gat Asp 225 t to Phe ctg Leu ggt Gly aac Asn gga Gly 305 ggc Gly ttc Phe gaa Giu ctg Leu ggc Gly 385 gcg Ala cag Gin gac Asp tct Ser 245 att Ile Gly tgt Cys atg Met tgc Cys 325 gac Asp t tc Phe gca Ala gaa Giu aac Asn 405 got Ala Met Arg Giu Asn Gly Giy Asp Gin Asp 1320 WO 01/00843 WO 0100843PCT/EBOO/00923 410 gat gag cgc ctc cca atg agc gaa gct 1411 Asp Glu Arg Leu Pro Met Ser Glu Ala 425 430 gac cgc cac gcc ttc atc ggt gcc gct 1459 Asp Arg His Ala Phe Ile Gly Ala Ala 440 445 ctc gac cgc att cag gtg ctt gtc gac 1507 Leu Asp Arg Ilie Gin Val Leu Val Asp 455 460 tac cga cca ggt gag att ctt taaaggtt 1551 Tyr Arg Pro Gly Glu Ilie Leu 470 475 <210> 962 <211> 476 <212> PRT <213> Corynebacterium glutanicuxn <400> 962 Val Ala Asp Lys Lys Lys Ilie Ala Asn 1 5 Ser Ala Glu Leu Ser Asn Leu Trp Ser 25 Glu Arg Gin Leu Trp Ile Ala Val Met Val Glu Ile Pro Ala Glu Ala Ile Glu 55 Gin Val Asp Leu Ala Ser Ile Ala Asp 70 Asp Val Lys Ala Arg Ilie Giu Glu Phe His Ile His Lys Gly Met Thr Ser Arg 100 105 Gin Leu Gin Ilie His Arg Ser Leu Glu 115 120 Ala Val Val Ala Ala Ile Gly Ser Arg 130 135 Val Met Ala Gly Arg Ser His Asn Vai 145 150 Gly Lys Arg Phe Ala Thr Ala Ala Asp 415 gac Asp ctt gag gca gcg Leu Giu Ala Ala 435 420 ctg Leu gag tcc cag gtc tcc cgt gtg Glu Ser Gin Val Ser Arg Val 450 gcc cac ccc ggc gcc gca gac Aia His Pro Gly Ala Ala Asp 465 :tt taacggcgtt cac Val 10 Ala Lys Ser Arg Asn 90 Asp Leu Aia Ala Giu 170 Leu Glu Ala Tyr Glu 75 Ala Leu Val Ala Ala 155 Met Ser Giu Gin Giu Arg Leu Thr Arg Gin 140 Gin Leu Arg Ile Asp Val1 Thr Gly Asn 110 Lys Gin Thr Ala Tyr Ile Leu Ile Arg His Val Gly Ser Thr Leu Ala Met Gly Asp His Giu Glu Ile Leu Leu 160 Glu 1321 WO 01/00843 PCT/IB00/00923 Arg Val Thr Glu Leu Leu Asn Arg Tyr Pro Leu Arg Gly Ile Lys Gly 180 185 190 Pro Met Gly Thr Ala Gin Asp Met Leu Asp Leu Met Glu Gly Asp Glu 195 200 205 Ala Arg Leu Ser Asp Leu Glu Thr Arg Ile Ala Ala His Leu Gly Phe 210 215 220 Asp Arg Val Phe Asp Ser Val Gly Gin Val Tyr Pro Arg Ser Leu Asp 225 230 235 240 Phe Asp Ala Val Ser Ala Leu Val Gin Leu Gly Ser Gly Pro Ser Ser 245 250 255 Leu Ser His Thr Ile Arg Leu Met Ala Gly Thr Glu Thr Val Thr Glu 260 265 270 Gly Phe Lys Glu Gly Gin Val Gly Ser Ser Ala Met Pro His Lys Met 275 280 285 Asn Ala Arg Ser Cys Glu Arg Val Gly Gly Leu Gin Val Ile Leu Arg 290 295 300 Gly Tyr Leu Thr Met Val Ala Asp Leu Ser Gly Gin Gin Trp Asn Glu 305 310 315 320 Gly Asp Val Phe Cys Ser Val Ile Arg Arg Val Ala Leu Pro Asp Ala 325 330 335 Phe Phe Ala Ile Asp Gly Met Phe Glu Thr Phe Leu Thr Val Leu Asp 340 345 350 Glu Phe Gly Ala Phe Pro Ala Met Ile Glu Arg Glu Leu Glu Arg Tyr 355 360 365 Leu Pro Phe Leu Ala Thr Thr Arg Ile Leu Met Ala Ala Val Arg Ala 370 375 380 Gly Val Gly Arg Glu Thr Ala His Glu Val Ile Lys Glu Asn Ala Val 385 390 395 400 Ala Val Ala Leu Asn Met Arg Glu Asn Gly Gly Asp Gin Asp Leu Ile 405 410 415 Gin Arg Leu Ala Ala Asp Glu Arg Leu Pro Met Ser Glu Ala Asp Leu 420 425 430 Glu Ala Ala Leu Ala Asp Arg His Ala Phe Ile Gly Ala Ala Glu Ser 435 440 445 Gin Val Ser Arg Val Leu Asp Arg Ile Gin Val Leu Val Asp Ala His 450 455 460 Pro Gly Ala Ala Asp Tyr Arg Pro Gly Glu Ile Leu 465 470 475 <210> 963 <211> 666 1322 WO 01/00843 WO 0100843PCT/IBOO/00923 <212> DNA <213> Corynebacteriun giutamicum <220> <221> CDS <222> (101)..(643) <223> RXA00688 gcggaaccgc aatcttgatt cttgtatctg ttgcactgac gccagctcct gcaaagcaac tacgaaggac ttctaaaata cacagtgaag cagattgaga atg cga ctc gta ctc ii! Met Arg Leu Val Leu ctc gga cct ccc Leu Giy Pro Pro gct ggt aag ggc acc cag gct gca att Ala Gly Lys Gly Thr Gin Ala Ala Ile ctc tct Leu Ser gag aag ctt Giu Lys Leu aac att ggc Asn Ile Giy att cct cac att Ile Pro His Ile act ggc gat ctt Thr Gly Asp Leu ttc cgc gcc Phe Arg Ala cag tac atc Gin Tyr Ile gaa ggt acc cct ctg ggt atc gag gcc Giu Giy Thr Pro Leu Giy Ile Giu Aia gac gct Asp Ala ggc aag ctg gtt Gly Lys Leu Val cca Pro acc gac gtg act Thr Asp Vai Thr cgt atg gtt gct Arg Met Val Ala 259 307 355 403 tcc Ser cgc ctt gct gag Arg Leu Ala Giu tcc gat gct gca gaa ggc ttc ctt ttg gat ggt Ser Asp Ala Ala Giu Gly Phe Leu Leu Asp Gly 75 80 ttc cca cgc acc Phe Pro Arg Thr gag cag gct gac atc ttg gct aac ctg Giu Gin Ala Asp Ile Leu Ala Asn Leu ctt tcc Leu Ser 100 gaa gct ggc Giu Ala Gly gac gtc gtc Asp Val Val 120 cag Gin 105 acc ctc gat ggt Thr Leu Asp Gly gt t Val1 110 gtt aac tac cag gtt tct gaa Val Asn Tyr Gin Vai Ser Glu gtg gag cgc atg Vai Giu Arg Met ctg Leu 125 tcc cgt ggt cgc gct gat gac aac Ser Arg Gly Arg Ala Asp Asp Asn 130 gaa gag Giu Giu 135 acc atc cgc acc Thr Ile Arg Thr cgt Arg 140 ctc ggt gtc tac Leu Gly Val Tyr cgc Arg 145 gac gag act gct Asp Giu Thr Ala cct Pro 150 ctg atc gat cac Leu Ile Asp His ggt gac aag atc atc aac att gag gct Gly Asp Lys Ile Ile Asn Ile Giu Ala 160 ggc gaa gtc gaa Gly Giu Vai Glu gag Giu 170 atc aac gct cgt Ile Asn Ala Arg ac c Thr 175 ctc aag gca ctg Leu Lys Ala Leu ggc aaa Gly Lys 180 taagatttct tctctagtgc tgc 1323 WO 01/00843 WO 0100843PCTIBOOIOO923 <210> 964 <211> 181 <212> PRT <213> Corynebacterium glutamicun <400> 964 Met Arg Leu Val Leu Leu Gly Pro Pro 1 Al a Asp Ala Ala Phe Ala Tyr Arg Arg 145 Asn Glu Asn Asp Ser Phe Glu Asp Glu Pro 150 Gly Gly 10 Ile Gly Leu Glu Val1 Thr Glu Arg His Glu 170 Gly Ser Gly Asp Ala Asp Val 110 Ser Gly Lys Arg Thr Thr I le Val Glu I le Val Arg Val1 I le Thr 175 Lys Ala Leu <210> 965 <211> 531 <212> DNA <213> Corynebacteriun glutamicun <22 0> <221> CDS <222> (101)..(508) <223> RXA00266 <400> 965 agtaggggat cgtccagcga agcggtcgca gaggctgtga ggaagctcta agtcgactta agtgcgcgaa gcagaccacc attaggtaga atcacccaac atg act gaa cgt act 115 Met Thr Glu Arg Thr 1 1324 WO 01/00843 ctc atc ctt atc aag cca gac ggt gtt 8cc Leu Ile Leu Ile Lys Pro Asp Gly Val Thr 15 atc atc gca cgt att gag cgc aag ggc ctg Ilie Ile Ala Arg Ile Giu Arg Lys Gly Leu ctg cgt gtt gca gac cgc gag acc gct gaa Leu Arg Val Ala Asp Arg Glu Thr Ala Giu gct gac aag cca ttc ttc ggt gag ctc gtt Ala Asp Lys Pro Phe Phe Gly Glu Leu Val cct ctg atc gca ggc atc gtc gaa ggc gag Pro Leu Ilie Ala Gly Ilie Val Giu Giy Giu 75 cgt cag ctt gct ggt ggc acc gac cca gtt Arg Gin Leu Ala Giy Giy Thr Asp Pro Val 95 acc atc cgc ggc gat ttc gca ctg act gtt Thr Ilie Arg Gly Asp Phe Ala Leu Thr Vai 105 110 ggt tct gat tcc cca gag tcc gct gag cgc Gly Ser Asp Ser Pro Giu Ser Ala Giu Arg 120 125 cct aac ctg taatttttac ggttagaaaa aaa Pro Asn Leu 135 <210> 966 <211> 136 <212> PRT <213> Corynebacteriun giutamicum <400> 966 Met Thr Glu Arg Thr Leu Ile Leu Ile Lys 1 5 Gly His Val Gly Glu Ilie Ilie Ala Arg Ile Leu Ala Ala Leu Asp Leu Arg Val Ala Asp His Tyr Giu Giu His Ala Asp Lys Pro Phe 55 Phe Ile Thr Ser Ala Pro Leu Ile Ala Gly 70 Ala Ilie Asp Ala Trp Arg Gin Leu Ala Giy aac Asn aag Lys aag Lys gaa Giu cg t Arg 80 gc t Aia gga Gly gag Glu.

PCT/IBO0100923 ggc gaa 163 Gly Giu ctg gat 211 Leu Asp gag cac 259 Giu His tct gca 307 Ser Ala gca tgg 355 Ala Trp cca ggc 403 Pro Gly 100 gtt cac 451 Val His tgg ttc 499 Trp, Phe Asp Arg Giu Gly Val1 Thr Asn Lys Lys Glu Arg Ala 1325 WO 01/00843 WO 0100843PCTJLB00100923 Lys Ala Thr Pro Gly Thr Ile Arg Gly 100 105 Giu Asn Val Val His Gly Ser Asp Ser 115 120 Ile Ser Ile Trp Phe Pro Asn Leu 130 135 Asp Phe Ala Leu Thr Val Gly 110 Giu Arg Giu Pro Giu Ser <210> <211> <212> <213> <220> <221> <222> <223> 967 1245

DNA

Corynebacterium

CDS

(101)..(1222) RXA00489 giutamicum <400> 967 cagcccgatt tcttcattga aatcgggctg tttctggttg tgtcgcgtat ctcgggtaaa gtcttcgtcg tatacgacca tttaagggag gcccgtcaca atg cgt gac cac Met Arg Asp His 1 gaa atc ggt atc Giu Ilie Gly Ilie cgt gag gca cga Arg Giu Ala Arg acc tac agc ttg Thr Tyr Ser Leu gac gat Asp Asp att tct gtc Ile Ser Val acc act tgg Thr Thr Trp tct agc cgc cgc Ser Ser Arg Arg cgt tca tcc aaa Arg Ser Ser Lys gat gtc gac Asp Val Asp ttc atg aat Phe Met Asn cat att gac gcc His Ile Asp Ala aag ttt gat ctg Lys Phe Asp Leu cac cca His Pro agt gat gca ttg Ser Asp Ala Leu agc cct gag ttt Ser Pro Giu Phe att gaa atg ggc Ile Giu Met Gly aag Lys cag ggt ggc ctt Gin Gly Gly Leu ggc Gly gtg atc aac gct Val Ile Asn Ala ggt ctg tgg ggt Gly Leu Trp Gly cat gct gat ctc His Ala Asp Leu gag gcg atc gca aag gtg att gct gcg Glu Ala Ile Ala Lys Val Ile Ala Ala tat gag Tyr Glu 100 gaa ggc gac cag gct gca gcc act Giu Gly Asp Gin Ala Ala Ala Thr 105 act ctt cag gag ctg cac gca Thr Leu Gin Giu Leu His Ala 115 gcg cca ctg Ala Pro Leu 120 gat act gag ctg Asp Thr Giu Leu ctg Leu 125 agt gag cgc att Ser Giu Arg Ile cag gtt cgt Gin Val Arg gat tcc ggt gag atc gtt gct gtg cgc gtg tct cca caa aat gtt cgt Asp Ser Gly Giu Ile Val Ala Val Arg Val Ser Pro Gin Asn Val Arg 1326 WO 01/00843 PCTIBOOIOO923 135 140 145 gag atc gca cca atc gtc atc aag gca ggt gct gat ctg ctg gtt atc 595 Glu Ilie Ala Pro Ile Val Ile Lys Ala Gly Ala Asp Leu Leu Val Ile 150 155 160 165 cag ggc acc ctg atc tct gca gag cac gtc aac acc ggt gga gag gcc 643 Gin Gly Thr Leu Ile Ser Ala Giu His Val Asn Thr Gly Gly Giu Ala 170 175 180 ctg aac cta aag gaa ttc atc ggt tct ttg gat gtt cct gtc atc gct 691 Leu Asn Leu Lys Glu Phe Ile Gly Ser Leu Asp Val Pro Val Ile Ala 185 190 195 ggt ggc gtg aac gat tac acc acc gcg ttg cac atg atg cgt acc ggt 739 Gly Gly Val Asn Asp Tyr Thr Thr Ala Leu His Met Met Arg Thr Gly 200 205 210 gct gtg ggc atc atc gtc ggt ggc ggc gag aac acc aac agc cta gca 787 Ala Val Gly Ilie Ile Val Gly Gly Gly Glu Asn Thr Asn Ser Leu Ala 215 220 225 ttg ggc atg gag gta tcc atg gcc act gcg att gct gat gtc gct gcg 835 Leu Gly Met Glu Val Ser Met Ala Thr Ala Ile Ala Asp Val Ala Ala 230 235 240 245 gca cgt cgt gat tac ctg gat gag acc ggt gga cgt tac gtg cac atc 883 Ala Arg Arg Asp Tyr Leu Asp Glu Thr Gly Gly Arg Tyr Val His Ile 250 255 260 att gca gat gga agc att gaa aac tcc ggt gat gta gtc aag gct att 931 Ilie Ala Asp Gly Ser Ile Glu Asn Ser Gly Asp Val Val Lys Ala Ile 265 270 275 gcc tgt ggc gca gat gct gtg gtg ctg ggt tca ccg ttg gct cgc gct .979 Ala Cys Gly Ala Asp Ala Val Val Leu Gly Ser Pro Leu Ala Arg Ala 280 285 290 gaa gaa gct gct gga aag ggc tac ttc tgg cca gca gtg gca gcg cac 1027 Glu Glu Ala Ala Gly Lys Gly Tyr Phe Trp Pro Ala Val Ala Ala His 295 300 305 cct cgt ttc cca cgc ggt gtg gtt act gag tcc gtg gac ttg gat gag 1075 Pro Arg Phe Pro Arg Gly Val Val Thr Giu Ser Val Asp Leu Asp Giu 310 315 320 325 gca gca cca agc ttg gag cag att ctg cat ggt ccg tct acg atg ccg 1123 Ala Ala Pro Ser Leu Giu Gin Ile Leu His Gly Pro Ser Thr Met Pro 330 335 340 tgg ggt gtg gaa aac ttc gaa ggt gga tta aag cgt gcg ctg gct aag 1171 Trp Gly Val Giu Asn Phe Glu Gly Gly Leu Lys Arg Ala Leu Ala Lys 345 350 355 tgt ggc tac acc gat ttg aag agc ttc caa aag gta agc ctg cac gtt 1219 Cys Gly Tyr Thr Asp Leu Lys Ser Phe Gin Lys Val Ser Leu His Val 1327 WO 01/00843 WO 0100843PCTIBOO/00923 360 365 aac taggtgtgtg tactcgcctc ttc 1245 Asn <210> 968 <211> 374 <212> PRT <213> Corynebacterium glutamicun <400> 968 Met Arg Asp His Val Giu Ile Gly Ile 370 1 Tyr Ser Leu Val1 Gly Ile Gin Ile Pro 145 Asp Thr Val Met Thr 225 Ala Ser Lys Pro Ile Leu Ala Giu Ala 130 Gin Leu Gly Pro Met 210 Asn Asp Ser Thr Pro Gin Ala Gly Pro Ser 135 Ile Giy Asn Gly Val 215 Gly Arg Val1 Trp Ser Gly Asp Asp Leu 120 Gly Ala Thr Leu Val1 200 Gly Met Arg Val His Asp Giy Leu Gin 105 Asp Glu Pro Leu Lys 185 Asn Ile Glu Asp Gly Ser Ile Al a Leu Asp Al a Thr Ile Ile Ile 170 Glu Asp Ile Val Tyr 250 Arg Ser Asp Leu Gly 75 Glu Ala Giu Val1 Val1 155 Ser Phe Tyr Val Ser 235 Leu Thr Ser Asp Phe Giu Val1 Leu Arg Ser Al a 160 Asn Asp His Asn Ile 240 Gly Val Ala Ala 245 1328 WO 01/00843 WO 0100843PCTIiBOO/00923 Arg Tyr Val His Ile Ile Ala Asp Gly Ser Ile 260 265 Val Val Lys Ala Ilie Ala Cys Gly Ala Asp Ala 275 280 Pro Leu Ala Arg Ala Glu Glu Ala Ala Gly Lys 290 295 Ala Val Ala Ala His Pro Arg Phe Pro Arg Gly 305 310 315 Val Asp Leu Asp Glu Ala Ala Pro Ser Leu Glu 325 330 Pro Ser Thr Met Pro Trp Gly Val Glu Asn Phe 340 345 Arg Ala Leu Ala Lys Cys Gly Tyr Thr Asp Leu 355 360 Val Ser Leu His Val Asn 3.7 0 <210> 969 <211> 1545 <212> DNA <213> Corynebacterium glutaxnicum <22 0> <221> CDS <222> (101)..(1531) <223> RXN02281 <400> 969 aagatcaaca acgccgccga ggtcaacttg gaaattatca cctataaaag cacagttttg aatccacagg gcatcagggc Gly Gly Trp Glu His 335 Leu Gin Lys cgc Arg atc Ile gac Asp gtg Val gat Asp gca aca Ala Thr acc tcg Thr Ser gca tac Ala Tyr tgg aaa Trp Lys ggg cga Gly Arg acg atc gat Thr Ile Asp acc aaa ctg Thr Lys Leu gcc gat gtt Ala Asp Val 45 ccc atc gac Pro Ile Asp tac tcc gcc Tyr Ser Ala 1329 gac Asp gcc Ala gtt Val cgc Arg acc Thr cgtgaggatt cttgcatacg gtg cag aaa gat agt Val Gin Lys Asp Ser 1 gca atc gcg aag ctc Ala Ilie Ala Lys Leu aaa gaa acc ctc aac Lys Glu Thr Leu Asn tac ccc aaa ctc acc Tyr Pro Lys Leu Thr acc gaa ccc ttc ggc Thr Giu Pro Phe Gly ttg tcc aaa cca cgc Leu Ser Lys Pro Arg WO 01/00843 WO 0100843PCTJIBOOIOO923 gtg att gag cgt Val Ile Glu Arg ctc cgc gaa caa Leu Arg Glu Gin ctc Leu gag cgt ctc acc Glu Arg Leu Thr agt aat Ser Asn 100 tat ccc tgc Tyr Pro Cys gag tac at Glu Tyr Ilie 120 aag Lys 105 att tac gta tct Ile Tyr Val Ser gag Giu 110 tca gat atc cgc Ser Asp Ile Arg atc cca ccg Ile Pro Pro 115 cgt gct ggt Arg Ala Gly cgc ggc gca cct Arg Gly Ala Pro gct acc gaa gct Ala Thr Giu Ala gat gtt Asp Val 135 gca gat atc arc Ala Asp Ile Ile cgc ccc acc ctg gat gaa gtc cac gac Arg Pro Thr Leu Asp Giu Val His Asp 145 gca Ala 150 att atc gac ggc Ile Ile Asp Giy tgg cac gcc ttc Trp His Ala Phe aac Asn 160 ggc ccc gaa ctc Gly Pro Giu Leu ctt ttc cac ttc Leu Phe His Phe ggg Gly 170 ccg caa cgc ttc Pro Gin Arg Phe gac Asp 175 atc gcc tgc gcc Ile Ala Cys Ala cgc arc Arg Ile 180 gag cac tac Glu His Tyr ttc acc aac Phe Thr Asn 200 acc Thr 185 ggc atc aac gtg Gly Ile Asn Val cac gtg cag aag His Val Gin Lys tac att crg Tyr Ile Leu 195 ttt gcc atg Phe Ala Met tac gcc atg cac Tyr Ala Met His acc gag ttc gtg Thr Giu Phe Val cat His 210 tcc gaa Ser Giu 215 ctc acc tcg gaa Leu Thr Ser Giu tcc cgc tac gtg Ser Arg Tyr Val ggt Gly 225 cta tcc ttg cca Leu Ser Leu Pro aac Asn 230 ggg cag gta att Gly Gin Val Ilie cga gag acc gcc Arg Giu Thr Ala agc ctc ggt acg Ser Leu Gly Thr acc ctt gat ctg Thr Leu Asp Leu act Thr 250 agc cgt ttc caa Ser Arg Phe Gin cct cgt tac gar Pro Arg Tyr Asp crc atc Leu Ile 260 acc gaa gcc Thr Giu Ala tcc aat gca Ser Asn Ala 280 ggt Gly 265 gac ggt att acc Asp Gly Ile Thr atc aac arc ggt Ilie Asn Ile Gly gtg ggc cca Vai Giy Pro 275 cgc cca gaa Arg Pro Giu aaa act atc acc Lys Thr Ilie Thr tgc ctt gct gtg Cys Leu Ala Val ctc Leu 290 gcc tgg gtg atg atc ggc cac tgt gcr ggc atg gac gcc cgc atg cgc 1027 Ala Trp, 295 arc ggc 1075 Val Met Ilie Gly Cys Ala Gly Met Asp 305 Ala Arg Met Arg gac ctc atc ctt ggc aac gcc tac cag cgc gaa gac cac at 1330 WO 01/00843 WO 0100843PCTJIBOO/00923 Ile Gly 310 ctg aat 1123 Leu Asn atc caa 1171 Ilie Gin aac agc 1219 Asn Ser tgg gaa 1267 Trp Giu 375 acc gcc 1315 Thr Ala 390 tat cga 1363 Tyr Arg cca cta 1412.

Pro Leu ttc aac 1459 Phe Asn tac ctg 1507 Tyr Leu 455 acc ttg 1545 Thr Leu 470 Asp Leu Ile acc cgc atc Thr Arg Ile 330 Gly Asn Ala Tlyr Gin 320 Arg Glu Asp His ctt ggt aat ccg atc Leu Gly Asn Pro Ile 335 gcc agc gtc gac gaa Ala Ser Val Asp Giu aaa gct Lys Ala 345 ctc atg Leu Met cta gaa Leu Glu ccg gca ata cca gaa Pro Ala Ile Pro Giu 340 atc tac gga tcc gac Ile Tyr Gly Ser Asp 355 acc gac gac cga aac Thr Asp Asp Arg Asn 350 cgc acc ggt acg gtc Arg Thr Giy Thr Val tta tcc Leu Ser 360 tgg Trp cac acc cca gaa His Thr Pro Giu 380 ctc Leu 370 tgg aac tgg ctc aaa gga tcc Trp Asn Trp Leu Lys Gly Ser 385 gca gct gtt Ala Ala Val ttc cgc att Phe Arg Ile 410 gac Asp 395 atg Met gaa tct tcc acc Glu Ser Ser Thr 400 t tg Leu gcc acc aac gga Ala Thr Asn Gly 405 cca tac ggc acc ctg Pro Tyr Giy Thr Leu 415 ccg aaa ctt tcc gcg Pro Lys Leu Ser Ala ctg agc gtc tct gac ctg Leu Ser Val Ser Asp Leu 420 caa gcg cag gcg ttt tac Gin Ala Gin Ala Phe Tyr 435 cac gca His Ala 425 tcc aag Ser Lys gtg Val gaa gcc cac gtc Giu Ala His Val 430 gca gta gat cct gaa cgg Ala Val Asp Pro Giu Arg 460 ggt gag gtg ccg ttt cgc Giy Giu Vai Pro Phe Arg 475 atg tgt gct gtt cgt gca atg gaa Met Cys Ala Val Arg Ala Met Giu 450 ttg cgt acc cgt aaa ctg cgc agg Leu Arg Thr Arg Lys Leu Arg Arg 465 taaagtctcg gaga <210> 970 <211> 477 <212> PRT <213> Corynebacterium giutamicun <400> 970 Val Gin Lys Asp Ser Val Val Arg Met Glu Ala Thr Thr Ile Asp Asp 1331 WO 01/00843 PCT/IB00/00923 1 5 10 Ala Ile Ala Lys Leu Ile Asp Ile Tyr Asp Thr Ser Thr Lys Leu Ala 25 Lys Glu Thr Leu Asn Asn Glu Asp Tyr Ala Ala Tyr Ala Asp Val Val 40 Tyr Pro Lys Leu Thr Val Asp Val Leu Glu Trp Lys Pro Ile Asp Arg 55 Thr Glu Pro Phe Gly Tyr Val Asp Arg Ala Gly Arg Tyr Ser Ala Thr 70 75 Leu Ser Lys Pro Arg Val Ile Glu Arg Tyr Leu Arg Glu Gin Leu Glu 90 Arg Leu Thr Ser Asn Tyr Pro Cys Lys Ile Tyr Val Ser Glu Ser Asp 100 105 110 Ile Arg Ile Pro Pro Glu Tyr Ile Arg Gly Ala Pro Ser Ala Thr Glu 115 120 125 Ala Arg Arg Ala Gly Asp Val Ala Asp Ile Ile Pro Arg Pro Thr Leu 130 135 140 Asp Glu Val His Asp Ala Ile Ile Asp Gly Asp Trp His Ala Phe Asn 145 150 155 160 Gly Pro Glu Leu Pro Leu Phe His Phe.Gly Pro Gin Arg Phe Asp Ile 165 170 175 Ala Cys Ala Arg Ile Glu His Tyr Thr Gly Ile Asn Val Glu His Val 180 185 190 Gin Lys Tyr Ile Leu Phe Thr Asn Tyr Ala Met His Thr Thr Glu Phe 195 200 205 Val His Phe Ala Met Ser Glu Leu Thr Ser Glu Asp Ser Arg Tyr Val 210 215 220 Gly Leu Ser Leu Pro Asn Gly Gin Val Ile Asp Arg Glu Thr Ala Thr 225 230 235 240 Ser Leu Gly Thr Glu Thr Leu Asp Leu Thr Ser Arg Phe Gin Met Pro 245 250 255 Arg Tyr Asp Leu Ile Thr Glu Ala Gly Asp Gly Ile Thr Ile Ile Asn 260 265 270 Ile Gly Val Gly Pro Ser Asn Ala Lys Thr Ile Thr Asp Cys Leu Ala 275 280 285 Val Leu Arg Pro Glu Ala Trp Val Met Ile Gly His Cys Ala Gly Met 290 295 300 Asp Ala Arg Met Arg Ile Gly Asp Leu Ile Leu Gly Asn Ala Tyr Gin 305 310 315 320 Arg Glu Asp His Ile Leu Asn Thr Arg Ile Pro Leu Gly Asn Pro Ile 325 330 335 1332 WO 01/00843 WO 0100843PCT/IBOO/00923 Pro Ala Ile Pro Glu Ile Gin Lys Ala Leu Giu 340 345 Ilie Tyr Gly Ser Asp Asn Ser Leu Met Arg Thr 355 360 Thr Asp Asp Arg Asn Trp Glu Trp His Thr Pro 370 375 Trp Leu Lys Gly Ser Thr Ala Ala Ala Val Asp 385 390 395 Leu Ala Thr Asn Gly Tyr Arg Phe Arg Ilie Pro 405 410 Ser Val Ser Asp Leu Pro Leu His Ala Val Pro 420 425 Ala Gin Ala Phe Tyr Phe Asn Ser Lys Giu Ala 435 440 Val Arg Ala Met Glu Tyr Leu Ala Val Asp Pro 450 455 Arg Lys Leu Arg Arg Thr Leu Gly Glu Val Pro 465 470 475 .<210> 971 <211> 1191 <212> DNA <213> Corynebacterium giutamicum <22 0> <221> CDS <222> (101)..(1168) <223> FRXA02281 <400> 971 aagatcaaca acgccgccga ggtcaacttg gaaattatca cctataaaag cacagttttg aatccacagg gcatcagggc Ala Gly Glu 380 Met Tyr

LYS

His Glu 460 Phe Ser Thr 365 Asn Glu Gly Leu Val1 445 Arg Arg gtg Val att Ile aat Asn gt t Val1 gc a Ala ac c Thr gca Al a tgg Trp aca acg Thr Thr tcg acc Ser* Thr tac gcc Tyr Ala aaa ccc Lys Pro gat Asp ctg Leu gtt Val1 gac Asp gac Asp gcc Ala gt t Val cgc Arg cgtgaggatt cttgcatacg gtg cag aaa gat agt Val Gin Lys Asp Ser 1 gca atc gcg aag ctc Ala Ilie Ala Lys Leu aaa gaa acc ctc aac Lys Glu Thr Leu Asn tac ccc aaa ctc acc Tyr Pro Lys Leu Thr acc gaa ccc ttc ggc Thr Glu Pro Phe Gly 1333 WO 01/00843 WO 0100843PCTIBOO/00923 tat Tyr gtg gat cga gcc Val Asp Arg Ala ggg Gly cga tac tcc gcc Arg Tyr Ser Ala ttg tcc aaa cca Leu Ser Lys Pro gtg att gag cgt Val Ile Giu Arg ctc cgc gaa caa Leu Arg Giu Gin ctc Leu gag cgt ctc acc Glu Arg Leu Thr agt aat Ser Asn 100 tat ccc tgc Tyr Pro Cys gag tac att Giu Tyr Ile 120 att tac gta tct Ile Tyr Vai Ser tca gat atc cgc Ser Asp Ile Arg atc cca ccg Ile Pro Pro 115 cgt gct ggt Arg Ala Giy cgc ggc gca cct Arg Gly Ala Pro gct acc gaa gct Ala Thr Glu Ala cgc Arg 130 gat gtt Asp Val 135 gca gat atc atc Ala Asp Ilie Ilie cgc ccc acc ctg Arg Pro Thr Leu gat Asp 145 gaa gtc cac gac Giu Val His Asp att atc gac ggc Ile Ilie Asp Giy gac Asp 155 tgg cac gcc ttc Trp His Ala Phe aac Asn 160 ggc ccc gaa ctc Giy Pro Giu Leu ctt ttc cac ttc Leu Phe His Phe ggg Giy 170 ccg caa cgc ttc Pro Gin Arg Phe gac Asp 175 atc gcc tgc gcc Ile Ala Cys Ala cgc atc Arg Ile 180 gag cac tac Giu His Tyr ttc acc aac Phe Thr Asn 200 acc Thr 185 ggc atc aac gtg Gly Ile Asn Val cac gtg cag aag His Vai Gin Lys tac att ctg Tyr Ile Leu 195 ttt gcc atg Phe Ala Met tac gcc atg cac Tyr Ala Met His acc gag ttc gtg Thr Giu Phe Val tcc gaa Ser Giu 215 ctc acc tcg gaa Leu Thr Ser Glu gac Asp 220 tcc cgc tac gtg Ser Arg Tyr Vai gg t G ly 225 cta tcc ttg cca Leu Ser Leu Pro ggg cag gta att Gly Gin Vai Ilie gac Asp 235 cga gag acc gcc Arg Giu Thr Ala agc ctc ggt acg Ser Leu Giy Thr acc ctt gat ctg Thr Leu Asp Leu act Thr 250 agc cgt ttc caa Ser Arg Phe Gin cct cgt tac gat Pro Arg Tyr Asp ctc atc Leu Ile 260 acc gaa gcc Thr Giu Ala tcc aat gca Ser Asn Ala 280 ggt Gly 265 gac ggt att acc Asp Giy Ile Thr atc aac atc ggt Ile Asn Ile Gly gtg ggc cca Val Giy Pro 275 cgc cca gaa Arg Pro Glu aaa act atc acc Lys Thr Ile Thr tgc ctt gct gtg Cys Leu Ala Val ctc Leu 290 gcc tgg 1027 Ala Trp 295 gtg atg atc ggc cac tgt gct ggc atg gac gcc cgc atg cgc Val Met Ile Gly His 300 Cys Ala Gly Met Ala Arg Met Arg 1334 WO 01/00843 WO 0100843PCTIBOOIOO923 atc ggc gac ctc atc ctt ggc aac gcc tac cag cgc gaa gac 1075 Ile Gly Asp Leu Ile Leu Giy Asn Ala Tyr Gin Arg Glu Asp 310 315 320 ctg aat acc cgc atc cca ctt ggt aat ccg atc ccg gca ata 1123 Leu Asn Thr Arg Ilie Pro Leu Gly Asn Pro Ile Pro Ala Ile 330 335 atc caa aaa gct cta gaa gcc agc gtc gac gaa atc tac gga 1168 Ilie Gin Lys Ala Leu Giu Aia Ser Val Asp Giu Ile Tyr Gly 345 350 355 tagtattcta tagtgtcacc taa 1191 <210> 972 <211> 356 <212> PRT <213> Corynebacteriun glutarnicum cac att His Ile 325 cca gaa Pro Glu 340 tcc Ser <400> 972 Val Gin Lys Asp Ser Val Vai Arg Met 1 Ala Lys Tyr Thr Leu Arg Ilie Ala Asp 145 Gly Asp Giu Asp Val Ile Pro Tyr Val 135 Ile Phe Ile Asp Val Asp Giu Cys Ile 120 Ala Ile His Tyr Tyr Leu Arg Arg Lys 105 Arg Asp Asp Phe Giu 10 Asp Aia Glu Ala Tyr Ilie Gly Ile Giy Gly 170 Giy Asp Leu Val Asp Ala Leu Ser Thr Thr Phe Asp 175 His Asp Ala Val Arg Thr Giu Asp Giu Leu Asn 160 Ile Val Ala Cys Aia Arg Ile Glu His Tyr Thr 1335 WO 01/00843 WO 0100843PCTJIBOO/00923 Gin Lys Tyr Ie Leu Phe Thr Asn Tyr Ala met 195 200 Val His Phe Ala Met Ser Giu Leu Thr Ser Giu 210 215 Gly Leu Ser Leu Pro Asn Gly Gin Val Ilie Asp 225 230 235 Ser Leu Gly Thr Giu Thr Leu Asp Leu Thr Ser 245 250 Arg Tyr Asp Leu Ile Thr Giu Ala Gly Asp Gly 260 265 Ile Gly Val Gly Pro Ser Asn Ala Lys Thr Ile 275 280 Val Leu Arg Pro Giu Ala Trp Val Met Ilie Giy 290 295 Asp Ala Arg Met Arg Ile Gly Asp Leu Ile Leu 305 310 315 Arg Giu Asp His Ilie Leu Asn Thr Arg Ile Pro 325 330 Pro Ala Ilie Pro Giu Ile Gin-Lys Aia Leu Giu 340 345 Ilie Tyr Gly Ser 355 <210> 973 <211> 1302 <212> DNA <213> Corynebacterium giutamicun <22 0> <221> CDS <222> (101)..(1279) <223> RXA00147 <400> 973 attgcatata atgcaatgaa ttgaataaac tacattcagg ttttaaaaag gcagacacac gaaaggcgac aacagtcacc acc acc tac cag gga gtc acc gag atc gga tcc Thr Thr Tyr Gin Gly Val Thr Giu Ile Giy Ser 15 gtt ctt gca gac gga cgt acc ttc acc gga ttt Val Leu Ala Asp Gly Arg Thr Phe Thr Gly Phe 30 ggc acc acc ctt ggt gag gca gtg ttc act acc Thr 205 Ser Glu Phe Thr Asp 285 Cys Asn Gly Ser Thr Arg Thr Gin Ile 270 Cys Aia Aia Asn Val 350 gttatcaacc agccaatttc gtg agt aaa gac acc Val Ser Lys Asp Thr 1 gtt ccg gca tac ctg Vai Pro Ala Tyr Leu ggc ttt gga gct aic Gly Phe Gly Ala Ie gcc atg acc ggt tac 1336 WO 01/00843 Gly Thr Thr caa gaa acc Gin Glu Thr acc gca cca Thr Ala Pro tcc cgc gac Ser Arg Asp gca gca cgt Ala Ala Arg atg gca ggc Met Ala Gly 120 ctg gtt cgc Leu Val Arg 13.5 tec ggc get Ser Gly Ala 150 aag aat cag Lys Asn Gin gct gat gaa Ala Asp Giu gtc gtg gcc Val Val Ala 200 tct gca cgc Ser Ala Arg 215 gag gat ate Glu Asp Ile 230 cct ggc gat Pro Gly Asp gtt ctt gaa Val Leu Giu att ett ggc Ile Leu Gly Leu atg Met cag Gin Gly gtg Val1 105 cag Gin cac Hius gac Asp cca Pro acc Thr 185 tac Tyr ggt Gly aag Lys cct Pro gcc Ala 265 ege Giu gat Asp ggc Gly 75 att Ile aac Asn atc Ile cgc Arg cag Gin 155 atg Met gte Val ctg Leu cgc Arg tac Tyr 235 gca Ala att Ile ttc Ala cet Pro 60 aac Asn tgg Trp tgg Trp, gtc Val aat Asn 140 cgc Arg acc Thr atc Ile ggc Giy acc Thr 220 aac Asn gca Ala eca Pro gge Gly Vai1 Phe 45 tee tat Ser Tyr ace gge Thr Gly gtt gea Val Ala ege gee Arg Ala 110 gge ate Gly Ile 125 gaa ggt Glu Gly eca gtt Pro Vai ggc gca Gly Ala gaa get Giu Ala 190 att aag Ile Lys 205 gte ate Val Ile eca tea Pro Ser gac gte Asp Val ttc ttt Phe Phe 270 atg gag Met Glu Thr eae His tgg Trp ggC Gly ace Thr gge Giy tee Ser gaa Glu aae Asn 175 gaa Glu e aa Gin gtg Val Giy atg Met 255 gge Giy Thr ege Arg aae Asn 80 ctt Leu ace Thr gga Giy att Ile gaa Giu 160 etc Leu gge Giy aae Asn ect Pro gtg Val1 240 gtt Vali ate Ile Ala eag Gin gat Asp gtt Vai tee Ser ate Ile gea Ala 145 etc Leu tee Ser gaa Giu ace Thr get Ala 225 tte Phe gat Asp tge Cys Met at t Ile gag Giu ate le ttg Leu gae Asp 130 geg Aia gta Vai gtt Vali gag Giu eca Pro 210 gaa Glu ate Ile ate Ile t te Phe Thr gtt Vai gac Asp ege Arg cag Gin 115 ace Thr gge Gly gag Giu gag Glu ege Arg 195 egt Arg ace Thr tee Ser gte Val1 Giy 275 PCT/iBOO/00923 Gly Tyr gtg get 307 Val Ala aac gag 355 Asn Glu gae etc 403 Asp Leu 100 eag gaa 451 Gin Giu ege gca 499 Arg Aia ate ttc 547 Ile Phe ate gte 595 Ile Val 165 gte tet 643 Val Ser 180 eac ace 691 His Thr egt ttc 739 Arg Phe eca tte 787 Pro Phe aae gge 835 Asn Gly 245 ege gaa 883 Arg Glu 260 aae eag 931 Asn Gin ace tac aag etg aag Thr Tyr Lys Leu Lys t ,te gge Phe Gly Arg Ala Phe 1337 WO 01/00843 WO 0100843PCTIIBOO/00923 cac cgc 1027 His Arg 295 gac atc 1075 Asp Ilie 310 cag gaa 1123 Gin Giu ctc aac 1171 Leu Asn tac tcc 1219 Tyr Ser agc ccc 1267 Ser Pro 375 280 ggc atc aac Gly Ile Asn acc gcc cag Thr Ala Gin ttc gag acc Phe Giu Thr 330 gac ggc gtc Asp Gly Val 345 gtt cag tac Val Gin Tyr 360 ctg ttt gac Leu Phe Asp 285 gtg Val ggc Gly ggc Gly ggt Giy gag Giu 365 gt t aag Lys t tc Phe act Thr att Ile 350 gcc Ala gag atc Ile 305 aag Lys gtc Val aag Lys ggc Gly gat Asp 385 290 acc Thr gg t Gly acc Thr tcc Ser cca Pro 370 gca Ala aag Lys gca Ala acc Thr 340 cgc Arg gat Asp gct Ala atc Ile Gly 325 tgc Cys gca Ala gca Ala cag Gin Gin Phe Val Giu Leu Met aag aaa ggc gca taaataacat gccaaz 1302 Lys Lys Giy Ala 390 <210> 974 <211> 393 <212> PRT <213> Corynebacterium giutamicum <400> 974 Vai Ser Lys Asp Thr Thr Thr TyrC 1 Vai Pro Ala Tyr Leu Vai Leu Aia 1 Giy Phe Gly Ala Ilie Gly Thr Thr I Ala Met Thr Gly Tyr Gin Giu Thr b~ Gin Ilie Vai Vai Ala Thr Ala ProC Asp Giu Asp Asn Giu Ser Arg AspC Igcgt tca ;in ~sp .eu det ;ln fly 1338 WO 01/00843 PCT/IB00/00923 Val Ile Arg Asp Leu Ala Ala Arg Val Ser Asn Trp Arg Ala Thr Thr 100 105 110 Ser Leu Gin Gin Glu Met Ala Gly Gin Gly Ile Val Gly Ile Gly Gly 115 120 125 Ile Asp Thr Arg Ala Leu Val Arg His Leu Arg Asn Glu Gly Ser Ile 130 135 140 Ala Ala Gly Ile Phe Ser Gly Ala Asp Ala Gin Arg Pro Val Glu Glu 145 150 155 160 Leu Val Glu Ile Val Lys Asn Gin Pro Ala Met Thr Gly Ala Asn Leu 165 170 175 Ser Val Glu Val Ser Ala Asp Glu Thr Tyr Val Ile Glu Ala Glu Gly 180 185 190 Glu Glu Arg His Thr Val Val Ala Tyr Asp Leu Gly Ile Lys Gin Asn 195 200 205 Thr Pro Arg Arg Phe Ser Ala Arg Gly Val Arg Thr Val Ile Val Pro 210 215 220 Ala Glu Thr Pro Phe Glu Asp Ile Lys Gin Tyr Asn Pro Ser Gly Val 225 230 235 240 Phe Ile Ser Asn Gly Pro Gly Asp Pro Ala Ala Ala Asp Val Met Val 245 250 255 Asp Ile Val Arg Glu Val Leu Glu Ala Asp Ile Pro Phe Phe Gly Ile 260 265 270 Cys Phe Gly Asn Gin Ile Leu Gly Arg Ala Phe Gly Met Glu Thr Tyr 275 280 285 Lys Leu Lys Phe Gly His Arg Gly Ile Asn Val Pro Val Lys Asn His 290 295 300 Ile Thr Gly Lys Ile Asp Ile Thr Ala Gin Asn His Gly Phe Ala Leu 305 310 315 320 Lys Gly Glu Ala Gly Gin Glu Phe Glu Thr Asp Phe Gly Thr Ala Ile 325 330 335 Val Thr His Thr Cys Leu Asn Asp Gly Val Val Glu Gly Ile Ala Leu 340 345 350 Lys Ser Gly Arg Ala Tyr Ser Val Gin Tyr His Pro Glu Ala Ala Ala 355 360 365 Gly Pro Asn Asp Ala Ser Pro Leu Phe Asp Gin Phe Val Glu Leu Met 370 375 380 Asp Ala Asp Ala Gin Lys Lys Gly Ala 385 390 <210> 975 <211> 1059 1339 WO 01100843 WO 0100843PCTIiBOO/00923 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> (101)..(1036) <223> RXA00145 <400> 975 ccccaccgca cgcgcggaag acgtttccgt catgcttaca gaaatcgacg gccgcgatgc agtcacgctc acccgagaag actctgaagg ggattcctag atg aag cac ctc Met Lys His Leu 1 tcc att agc gat Ser Ile Ser Asp tcc aaa gat gag Ser Lys Asp Giu gtt gga ttg ctg Val Gly Leu Leu gat gaa Asp Giu gcg gat cgc Ala Asp Arg ttt Phe aag gag gtg ctc Lys Glu Val Leu gga cgt gaa gta Gly Arg Giu Val aag aag ctg Lys Lys Leu gag aac tcc Ciu Asn Ser ccc acg ctg cgt ggt cgc acc att ttt acc ttg ttc tat Pro Thr Leu Arg Gly Arg Thr Ile Phe Thr Leu Phe Tyr 45 acg cgc Thr Arg acc cgt tcg tcc Thr Arg Ser Ser gaa acc gca gga Giu Thr Ala Gly aag Lys tgg atg agc gcc Trp Met Ser Ala gtg att aac att Val Ilie Asn Ile gcc tca tca tcc Ala Ser Ser Ser gtg aag aag ggc Val Lys Lys Gly tcg ctg aaa gat Ser Leu Lys Asp ggc ttg act ttg Gly Leu Thr Leu gca atc ggc gcg Ala Ile Gly Ala gat gcg Asp Ala 100 atc atc atg Ile Ile Met ttc gtc gca Phe Val Ala 120 cgc Arg 105 cac cca gcc tca His Pro Ala Ser gcc gcg cag cag ctt gcg cag Ala Ala Gin Gin Leu Ala Gin cca ggt ggc aac ggc ccc agc gtg atc Pro Gly Gly Asn Gly Pro Ser Val Ile aac Asn 130 gcg ggt gac Ala Gly Asp ggt tcg Gly Ser 135 cac cag cac ccc His Gin His Pro cag gcg ctt ctc Gin Aia Leu Leu gct tta acc atc Ala Leu Thr Ile cgg Arg 150 cag cgc acc ggc cgc att gag gga ctc Gin Arg Thr Gly Arg Ile Giu Gly Leu 155 gtt gtc atc gtg Val Val Ile Val gac tgt ttg cac Asp Cys Leu His tcC S er 170 cgg gtg gtg cgc Arg Val Val Arg aat gtg gat ctg Asn Val Asp Leu ctg tcc Leu Ser 180 act ttg ggc gca gag gta gtg ctg gtt gct cct ccg aca ctg ctt cct Thr Leu Gly Ala Giu Val Val Leu Val Ala Pro Pro Thr Leu Leu Pro 1340 WO 01/00843 WO 0100843PCTIBOOIOO923 att ggt Ile Gly gaa att Gu- Ilie 215 cgc atg Arg Met 230 tac ggc Tyr Gly atc atg Ilie Met gtg gca Val Ala gtg cac 1027 gtg Val 200 gcc Ala cag Gin atg Met cac His gac Asp 280 atg 185 gag Glu gac Asp ggt Gly tcc Ser ccc Pro 265 gca Ala cgc t tc Phe atg Met cac His cgc Arg 255 ggc Gly ctg Leu gcc tcc Ser ctg Leu cgt Arg 240 ctc Leu atg Met cag Gin ctg Val His met Arg Met Ala Ile Leu Phe Ala Leu Val Ala Gly Ser Asp 295 300 305 gcg act atc taatcgcgac catctgatc 1059 Ala Thr Ilie 310 <210> 976 <2ii> 312 <212> PRT <213> Corynebacteriui glutamicum <400> 976 Met Lys His Leu Leu Ser Ile Ser 1 5 Gly Leu Leu Asp Glu Ala Asp Arg 1 Giu Val Lys Lys Leu Pro Thr Leu Phe Tyr Giu Asn Ser Thr Arg Thr P 55 Lys Trp Met Ser Ala Asp Val Ile Val Lys Lys Gly Glu Ser Leu Lys cga ~sp Li ~he L, 25 Lrg G ~rg S ~sn I Ls p TJ Ys ly e Glu Glu Phe Thr Ser Leu 1341 WO 01/00843 PCTIiBOO/00923 Ile Gly Ala Asp Ala Ile Ile Met Arg His Pro Ala Ser Gly Ala Ala 100 105 110 Gin Gin Leu Ala Gin Phe Val Ala Pro Gly Gly Asn Gly Pro Ser Val 115 120 125 Ile Asn Ala Gly Asp Gly Ser His Gin His Pro Thr Gin Ala Leu Leu 130 135 140 Asp Ala Leu Thr Ilie Arg Gin Arg Thr Gly Arg Ile Glu Gly Leu Lys 145 150 155 160 Val Val Ile Val Gly Asp Cys Leu His Ser Arg Val Val Arg Ser Asn 165 170 175 Vai Asp Leu Leu Ser Thr Leu Gly Ala Giu Val Val Leu Val Ala Pro 180 185 190 Pro.Thr Leu Leu Pro Ile Giy Val Giu Asn Trp Pro Val Arg Phe Ser 195 200 205 Tyr Asp Met Asp Ala Giu Ile Aia Asp Ala Asp Val Val Met Met Leu 210 215 220 Arg Vai Gin Gin Giu Arg Met Gin Gly Gly Phe Phe Pro Ser His Arg 225 230 235 240 Giu Tyr Ala Thr Leu Tyr Gly Met Ser Lys Giu Arg Giu Ala Arg Leu 245 250 255 Lys Asp Ser Ala Ile Ile Met His Pro Gly Pro Met Leu Arg Gly Met 260 265 270 Giu Ile Asn Phe Gin Val Ala Asp Ala Pro Arg Thr Ala Val Leu Gin -275 280 285 Gin Val Ser Asn Gly Val His Met Arg Met Ala Ile Leu Phe Ala Leu 290 295 300 Val Ala Giy Ser Asp Ala Thr Ile 305 310 <210> 977 <211> 1464 <212> DNA <213> Corynebacteritum giutamicum <22 0> <221> CDS <222> (101)..(1441) <223> RXA00i46 <400> 977 atggccattt tgttcgccct ggtcgcaggc tctgacgcga ctatctaatc gcgaccatct gatcgcgaaa ttctagaaaa ggtaacgagg gattaaagaa gtg gtt gac agc aac 115 Val Val Asp Ser Asn 1 acc cag tat cca gaa acc ggc gca ctg gca ccg gct cct gca gac tca 163 1342 WO 01/00843 Thr Gin Tyr Pro Glu Thr Gly Ala Leu Ala Pro Ala Pro Ala PCTAIBOO/00923 15 ctc Leu g tg Vai gct Ala gtg Val acc Thr gta Val gcg Ala cat His gag Glu 150 gat Asp gaa Glu cac His cgt Arg gtg Val 230 tgc Cys cta Leu ttt Phe gac Asp gac Asp att Ile ttc Phe gaa Giu cca Pro 135 ttc Phe gat Asp tac Tyr cgc Arg ctg Leu 215 cgc Arg c ac His atc Ile gtt Val cgc Arg atg Met gcc Ala acc Thr tcc Ser 120 gtt Val ggc Gly ggc Gly gcc Ala ctg Leu 200 ggt Gly gat Asp gcc Ala agc Ser aaa Lys acc Thr cac His act Thr atg Met 105 gta Val gga Gly atg Met aag Lys aag Lys 185 act Thr ctg Leu gcc Ala tcc Ser aac Asn gat Asp atc Ile gt t Val1 ggt Giy gcg Ala tgg Trp tcc Ser atg Met tgc Cys 170 ggc Gly gag Glu cgc Arg atc Ile act Thr gtc Val1 atc Ile 45 aat Asn cgt Arg gcc Ala act Thr ggc Gly 125 aag Lys tct Ser gat Asp gtt Val tca, Ser 205 cca Pro cgt Arg acc Thr ggc Gly gct Ala gga Gly cca Pro gcc Ala 95 gtg Vai aac Asn ctt Leu gcc Ala cag Gin 175 atc Ile cac His g tg Val tac Tyr gag Glu gaa Glu atc Ile gtt Val ggC Gly 80 aag Lys atg Met at t Ile gag Giu aag Lys 160 gtc Val gcc Ala gag Giu gct Ala ggc Gly 240 ggc Gly ggc Gly ctc Leu cgc Arg ggc Gly ga t Asp ggc Gly ggc Gly 145 gtg Val atg Met cag Gin ggt Gly gag Glu 225 aac Asn gag Giu Gly ctt Leu gaa Giu gga Gly cag Gin ctg Leu 130 aag Lys cgc Arg cgc Arg cac His gaa Glu 210 gaa Glu cgc Arg cca Pro act Thr cca Pro gac Asp t tc Phe ccg Pro 115 tgc Cys gag Glu atg Met cgc Arg gc t Ala 195 aac Asn tcc Ser gtg Val Asp acg Thr cat His ggt Gly act Thr acc Thr 100 gt t Val gac Asp ctt Leu ttc Phe gcg Ala 180 gag Giu gca Ala atc Ile cac His Ser aat Asn gag Giu ttc Phe gaa Giu gca Ala atc Ile gtg Val1 act Thr tct Ser 165 ctg Leu gat Asp gct Ala gtg Val1 atc Ile 245 ttg ctt cgt tgg gct aag Leu Leu Arg Trp Ala Lys 1343 WO 01/00843 tcc cag Ser Gin ttg acc Leu Thr ccg cca 1027 Pro Pro 295 ctc gac 1075 Leu Asp 310 tcc gaa 1123 Ser Glu gga ttg 1171 Gly Leu gga ctt 1219 Gly Leu gca gaa 1267 Ala Glu 375 gag cca 1315 Glu Pro 390 tcc ggt 1363 Ser Gly caa gaa 1411 Gin Glu act tgt 1461 Thr Cys ggC Gly gat Asp 280 c tg Leu ggc Gly gat Asp gaa Glu gca Ala 360 atc Ile gca Ala gca Al a ttC Phe gca Ala 440 acc Thr gaa Glu cgc Arg 300 gtt Val gaa Glu tcc Ser ttt Phe cca Pro 380 att Ile tcc Ser gtC Val1 gca Ala 255 gcg gaa gtc Ala Glu Val 270 acc tac gac Thr Tyr ASP 285 gat gcc gaa Asp Ala Glu gtt gca acc Val Ala Thr ttc gaa aac Phe Glu Asn 335 atc atc gtg Ile Ile Val 350 gtt gcg cgc Val Ala Arg 365 ggc cag ggt Gly Gin Gly gtt gat cca Val Asp Pro aag gct gaa Lys Ala Glu 415 aca cac acc Thr His Thr 430 caa aac gct Gin Asn Ala 445 ca c His 275 aaa Lys aag Lys cc t Pro ggc Gly gtt Val1 355 gaa Glu gca Ala tgg Trp ttt Phe ggc PCTIiBOO/00923 260 ctc acc 931 Leu Thr gtc aat 979 Val Asn gcg ctt Ala Leu cac ggt His Gly 325 atg ctc Met Leu 340 gcc acc Ala Thr cgc cca Arg Pro gaa ggt Glu Gly aca gca Thr Ala 405 gag ggc Glu Gly 420 aag gtg Val Leu Arg Gly Lys Val 435 taacgggtgg gtgcatagta 1344 WO 01/00843 PCTI/iBO0/00923 tgc 1464 <210> 978 <211> 447 <212> PRT <213> Corynebacteriun glutamicun <400> 978 Val Val Asp Ser Asn Thr Gin Tyr Pro Giu Thr Gly Ala Leu Ala Pro 1 5 10 Ala Pro Ala Asp Ser Leu Leu Ile Ser Asn Val Leu Val Tyr Giy Giu 25 Gly Giu Pro Thr Asn Val Phe Val Lys Asp Gly Val Ile Ala Ala Ile 40 Gly Gly Thr His Glu Ala Asp Arg Thr Ile Asp Gly Asn Gly Gly Val 55 Leu Leu Pro Gly Phe Val Asp Met His Val His Leu Arg Glu Pro Gly 70 75 Arg Giu Asp Thr Glu Thr Ile Ala Thr Giy Ser Ala Ala Ala Ala Lys 90 Gly Gly Phe Thr Ala Val Phe Thr Met Ala Asn Thr Thr Pro Val Met 100 105 110 Asp Gin Pro Val Ile Ala Glu Ser Val Trp Phe Lys Gly Gin Asn Ile 115 120 125 Gly Leu Cys Asp Val His Pro Val Gly Ser Ile Thr Lys Gly Leu Giu 130 135 140 Gly Lys Glu Leu Thr Glu Phe Gly Met Met Ala Arg Ser Giu Ala Lys 145 150 155 160 Val Arg Met Phe Ser Asp Asp Gly Lys Cys Val Asp Asp Pro Gin Val 165 170 175 Met Arg Arg Ala Leu Giu Tyr Ala Lys Gly Met Asp Val Leu Ile-Ala 180 185 190 Gin His Ala Giu Asp His Arg Leu Thr Glu Gly Ala Ser Ala His Glu 195 200 205 Gly Giu Asn Ala Ala Arg Leu Gly Leu Arg Gly Trp Pro Arg Val Ala 210 215 220 Giu Giu Ser Ile Val Val Arg Asp Ala Ile Met Ala Arg Asp Tyr Gly 225 230 235 240 Asn Arg Val His Ile Cys His Ala Ser Thr Giu Gly Thr Val Giu Leu 245 250 255 Leu Arg Trp Ala Lys Ser Gin Gly Ile Pro Ile Thr Ala Glu Val Thr 260 265 270 1345 WO 01/00843 Pro His His 275 Val Asn Lys 290 Leu Lys Lys 305 His Ala Pro Lys Pro Gly Thr Phe Val 355 met Ser Giu 370 Pro Ile Ala 385 Lys Thr Trp Thr Pro Phe Leu Arg Gly 435 Thr Pro 295 Asp Glu Leu Leu Glu 375 Pro Gly Giu Cys Asp 280 Leu Gly Asp Giu Ala 360 Ile Ala Ala Phe Ala 440 Glu Arg rhr Lys rhr 345 rhr ~Asn Asp Ser 425 cct Pro Leu 25 jcc kla Itt lal Arg Giu Ile Cys 330 Ser Trp Arg Leu Phe 410 Ala Gly cta Leu 10 tcc Ser gca Ala gga Gly Leu Ser Asp 315 Cys Leu Arg Leu Ala 395 Ala Lys Val1 aac Asn cag Gin ggt Gly t tc Phe Glu Arg 300 Val Glu Ser Phe Pro 380 Ile Ser Val Ala Cgc Arg gaa Giu t tc Phe gga Gly Thr 285.

Asp Val1 Phe Ile Val 365 Gly Val Lys Thr Gin 445 ac c Thr gtc Val gac Asp tac Tyr PCTIBOOIOO923 Asp Ala Glu Ala Thr Asp 320 Asn Ala 335 Val Asp Arg Val Gly Arg Pro Gly 400 Giu Asn 415 Thr Val Ala <210> 979 <211> 1025 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1002) <223> RXA02208 <400> 979 ctc gga gtc gtc gac ggc gtc gca Leu Gly Val Val Asp Gly Val Ala 1 5 atc atc gca gtg cac gat gat tcc Ile Ilie Ala Val His Asp Asp Ser acc ttc cca cga cca cta ggc ctc Thr Phe Pro Arg Pro Leu Gly Leu 40 tca atg gct gat gcc tgg ggt gcc Ser Met Ala Asp Ala Trp Gly Ala 55 atg gaa Met Giu ttc ggc Phe Gly aaa aac Lys Asn gcc gaa Ala Glu 1346 WO 01/00843 WO 0100843PCTIIBOO/00923 ggc Gly acc gtc acc gcc ttc cca cag cca gga Thr Val Thr Ala Phe Pro Gin Pro Gly ccc acc ccg cgc Pro Thr Pro Arg ttc cgc ctg cct Phe Arg Leu Pro gac aaa gct atc ttg aac cgc atg gga Asp Lys Ala Ile Leu Asn Arg Met Gly ttc aac Phe Asn aac ctg ggt Asn Leu Gly gca gaa gtc gca Ala Giu Val Ala aaa Lys 105 aac ctg cgc aac Asn Leu Arg Asn cgg aaa tcc Arg Lys Ser 110 acc gat gtc atc ggc atc aac Thr Asp Val Ile Gly Ile Asn 115 atc ggt aaa acc aaa gtg gtt ccc gct Ile Gly Lys Thr Lys Val Val Pro Ala 120 125 gaa cac Giu His 130 gca gta gat gac Ala Val Asp Asp tac Tyr 135 cgc cgt tct gca Arg Arg Ser Ala ttg tta ggt gat Leu Leu Gly Asp ctt Leu 145 gct gat tac ctg Ala Asp Tyr Leu gtc aac gtt tcc Val Asn Val Ser tcc Ser 155 ccc aac act ccg Pro Asn Thr Pro ggt Gly 160 ctc cgc gat ctg Leu Arg Asp Leu gct gtg gaa tct Ala Val Giu Ser cga cca atc ctc Arg Pro Ile Leu gcc gca Ala Ala 175 gtg cag gaa Val Gin Giu ctc tcc gac Leu Ser Asp 195 tcc Ser 180 acc acc gtc cca Thr Thr Val Pro ttg gtg aaa atc Leu Val Lys Ile gca cca gac Ala Pro Asp 190 gtt gag ctc Val Giu Leu gaa gac atc gac Giu Asp Ile Asp gta gct gac ctg Val Ala Asp Leu gca Ala 205 aaa ctc Lys Leu 210 gcc gga atc gta Ala Gly Ile Val acc aat acc acc Thr Asn Thr Thr tcc cgc gaa ggc Ser Arg Giu Gly aac act cct tca Asn Thr Pro Ser ggt Gly 230 gaa gtc gaa gcc Giu Val Giu Ala ggt gct ggc gga Gly Ala Gly Gly tcc ggt gct cca Ser Gly Ala Pro gta Val 245 gca gcc cga tct Ala Ala Arg Ser gag gta ctc aag Giu Val Leu Lys cgc ctc Arg Leu 255 tac gca cgg Tyr Ala Arg agc acc cct Ser Thr Pro 275 gta Val 260 ggc aaa gag atg Gly Lys Giu Met ttg atc tct gtc Leu Ile Ser Val ggt ggc atc Gly Gly Ile 270 gca acc ctt Ala Thr Leu gag caa gcc tgg Giu Gin Ala Trp, cgc atc acc tcc Arg Ile Thr Ser ctg cag Leu Gin 290 gga tac acc cca Gly Tyr Thr Pro ttc Phe 295 atc tac ggt ggc Ile Tyr Gly Gly gat tgg atc aga Asp Trp Ile Arg gat atc cac ctt ggt atc gcc aag cag ctg aaa gct cac ggt ctg cgc 1347 WO 01/00843 PCTIIBOO/00923 Asp Ilie His Leu Gly Ile Ala Lys Gin Leu Lys Ala His Gly Leu Arg 305 310 315 320 aac atc gct gac gct gtg ggc agc gaa ttg gag tgg aag aac 1002 Asn Ile Ala Asp Ala Val Gly Ser Glu Leu Giu Trp Lys Asn 325 330 taaacagacc aaacacacgt gcc 1025 <210> 980 <211> 334 <212> PRT <213> Corynebacterium giutamicun <400> 980 Leu Gly Val Val Asp Gly Val Ala Pro Leu Asn Arg Thr Met Glu Lys 1 5 10 Ile Ilie Ala Val His Asp Asp Ser Leu Ser Gin Glu Val Phe Gly Val 25 Thr Phe Pro Arg Pro Leu Gly Leu Ala Ala Gly Phe Asp Lys Asn Ala 40 Ser Met Ala Asp Ala Trp Gly Ala Val Gly Phe Gly Tyr Ala Glu Leu 55 Gly Thr Val Thr Ala Phe Pro Gin Pro Gly Asn Pro Thr Pro Arg Leu 70 75 Phe Arg Leu Pro Ala Asp Lys Ala Ile Leu Asn Arg Met Gly Phe Asn 90 Asn Leu Gly Ala Ala Glu Val Ala Lys Asn Leu Arg Asn Arg Lys Ser 100 105 110 Thr Asp Val Ilie Gly Ile Asn Ile Gly Lys Thr Lys Val Val Pro Ala 115 120 125 Glu His Ala Val Asp Asp Tyr Arg Arg Ser Ala Ser Leu Leu Gly Asp 130 135 140 Leu Ala Asp Tyr Leu Val Val Asn Val Ser Ser Pro Asn Thr Pro Gly 145 150 155 160 Leu Arg Asp Leu Gin Ala Val Glu Ser Leu Arg Pro Ile Leu Ala Ala 165 170 175 Val Gin Giu Ser Thr Thr Val Pro Val Leu Val Lys Ile Ala Pro Asp 180 185 190 Leu Ser Asp Giu Asp Ile Asp Ala Val Ala Asp Leu Ala Val Glu Leu 195 200 205 Lys Leu Ala Gly Ile Val Ala Thr Asn Thr Thr Ile Ser Arg Glu Gly 210 215 220 Leu Asn Thr Pro Ser Gly Giu Val Glu Ala Met Gly Ala Gly Gly Ile 1348 WO 01/00843 225 Ser Gly Ala Tyr Ala Arg Ser Thr Pro 275 Leu Gin Gly 290 Asp le His 305 Asn Ile Ala Pro Val1 260 Giu Tyr Leu Asp Val1 245 Gly Gin Thr Giy Ala 325 Leu 250 Leu Ile Giy Leu Leu 330 235 Giu Ile Thr Giy Lys 315 Giu PCTiIBOOIOO923 240 rg Leu ly Ile ,r Leu le Arg u Arg 320 <210> 981 <211> 675 <212> DNA <213> Corynebacterium giutainicum <2 <221> CDS <222> (652) <223> RXA01660 <400> 981 gaaaactggt gtttttcggc cgtgtccacc ccaggttcta taaacctcaa tcatcaaatt agggaagggc tgggaaatcc att aac gca gaa Ile Asn Ala Giu gct Ala tac Tyr ctg Leu gca Ala atg Met gcg Ala gg t Gly gtc Val c tg Leu ctg Leu ggc Gly cgc gct Arg Ala gaa gtc Giu. Val cgt cgt Arg Arg ctg cgc Leu Arg acc ttg Thr Leu 75 cgc gat Arg Asp tgctgtaaca aac.gcgggtt atg tca tct aat tcc Met Ser Ser Asn Ser 1 ctg atc aaa gag cta Leu Ile Lys Giu Leu ggc aag aag gct gat Gly Lys Lys Ala Asp gcg cgc gca tct cgc Ala Arg Ala Ser Arg gac tgg gac tat gac Asp Trp Asp Tyr Asp gtt gcc acc gcc atc Val Ala Thr Ala Ile gtg gtg cgc aag gag Val Val Arg Lys Giu 100 115 163 211 259 307 355 403 1349 WO 01/00843 WO 0100843PCTIBOOIOO923 gec aag aag cac ggc atg cag cgt cgc att gag ggc cct gac ctg acg 451 Ala Lys Lys His Gly Met Gin Arg Arg Ilie Giu Gly Pro Asp Leu Thr 105 110 115 ggc aag aag gtg ctc gtg gtg gaa gat acc acc acc acc gga aat tcc 499 Gly Lys Lys Val Leu Val Val Giu Asp Thr Thr Thr Thr Gly Asn Ser 120 125 130 cct ctg aca gct gtt gcc gcg ttg cgt gaa gct ggc att gag gtt gtg 547 Pro Leu Thr Ala Val Ala Ala Leu Arg Giu Ala Gly Ile Giu Val Val 135 140 145 ggc gtt gcc acc gtg gtc gat cgc gca acc ggt gca gat gag gtt atc 595 Gly Val Ala Thr Val Val Asp Arg Ala Thr Gly Ala Asp Giu Val Ile 150 155 160 165 gca gcg gaa ggc ctt cct tac cgc agc ttg ctg gga ctt tct gat ctt 643 Ala Ala Giu Gly Leu Pro Tyr Arg Ser Leu Leu Gly Leu Ser Asp Leu 170 175 180 gga ctc aac taacaccccc ggccccacgg agt 675 Gly Leu Asn <210> 982 <211> 184 <212> PRT <213> Corynebacteriun giutarnicum <400> 982 Met Ser Ser Asn Ser Ile Asn Ala Glu Ala Arg Ala Giu Leu Ala Glu 1 5 10 Leu Ilie Lys Giu Leu Ala Val Val His Gly Giu Val Thr Leu Ser Ser 25 Gly Lys Lys Ala Asp Tyr Tyr Ile Asp Val Arg Arg Ala Thr Leu His 40 Ala Arg Ala Ser Arg Leu Ile Gly Gin Leu Leu Arg Giu Ala Thr Ala 55 Asp Trp Asp Tyr Asp Ala Val Gly Gly Leu Thr Leu Gly Ala Asp Pro 70 75 Val Ala Thr Ala Ile Met His Ala Asp Gly Arg Asp Ile Asn Ala Phe 90 Val Val Arg Lys Glu Ala Lys Lys His Gly Met Gin Arg Arg Ile Giu 100 105 110 Gly Pro Asp Leu Thr Gly Lys Lys Val Leu Val Val Giu Asp Thr Thr 115 120 125 Thr Thr Gly Asn Ser Pro Leu Thr Ala Val Ala Ala Leu Arg Giu Ala 130 135 140 dly Ile Giu Val Val Gly Val Ala Thr Val Val Asp Arg Ala Thr Gly 145 150 155 160 1350 WO 01/00843 WO 0100843PCTIIIBOO/00923 Ala Asp Glu Val Ile Ala Ala Glu Gly Leu Pro Tyr Arg Ser Leu Leu 165 170 175 Gly Leu Ser Asp Leu Gly Leu Asn 180 <210> 983 <211> 957 <212> DNA <213> Corynebacterium glut amic ux <220> <221> <222> <223>

CDS

(101)..(934) RXA0 2235 <400> 983 gtgicaccgc agctgtccag ggcatagagg ccc tgcgtga gggcgttgtc agcgtccgcg cgctgcagga actcgaccac gcagtcaagg cttaagccct atg aca tic ggc Met Thr Phe Gly 1 aag cti ctg aac Lys Leu Leu Asn gcc Ala gcc icc acc cgt Ala Ser Thr Arg ggc Gly 15 agg cta tgc gig Arg Leu Cys Val ggc ati Gly Ile gat ccc cac Asp Pro His gac gga cii Asp Gly Leu agc cig ctg acg Ser Leu Leu Thr tgg ggg ctg ccg Trp Gly Leu Pro gta aac gta Val Asn Val tic gcc gac Phe Ala Asp gcg gag tic icc Ala Glu Phe Ser gcc igc gig gag Ala Cys Val Glu acc gig Thr Val1 gca tig gig aag cci cag gig gcg tic Ala Leu Val Lys Pro Gin Val Ala Phe gag cgt tic ggi Glu Arg Phe Gly gci ggc iii gcc Ala Gly Phe Ala tig gaa gaa acc Leu Glu Glu Thr at Ile cag acg cig cgi Gin Thr Leu Arg cgi ggc igi tig Arg Gly Cys Leu gig Val gic ici gac gcc Val Ser ASP Ala cgc ggc gat at Arg Gly ASP Ile ggc icc Gly Ser 100 acc aig gci Thr Met Ala ici agc gac Ser Ser Asp 120 tat gcc ica gcg Tyr Ala Ser Ala tia gat cca gcg ica ccg cig Leu Asp Pro Ala Ser Pro Leu 115 gci gig acg gic Ala Val Thr Val ccc tac cii ggi Pro Tyr Leu Gly iii Phe 130 cat icc ttg His Ser Leu gac cca Asp Pro 135 gig iic gaa cit gcc gag caa cac ggc Val Phe Giu Leu Ala Glu Gin His Gly 140 agg Arg 145 gga gig iii gic Gly Val Phe Val 1351 WO 01/00843 ttg gcc gcg acc tca aac cct Leu Ala Ala Thr Ser Asn Pro gag gcc cgc gaa ctc cag gac Giu Ala Arg Giu Leu Gin Asp 150 aac gct gac ggc Asn Ala Asp Gly gcg ctt aac gcg Ala Leu Asn Ala 185 gtc gtc atc ggc Val Val Ile Gly 200 ggg ggc gcc att Gly Gly Ala Ile 215 agc gac gtt gat Ser Asp Val Asp 230 aat gtc tct aga Asn Val Ser Arg aag aat tct gtg Lys Asn Ser Val .265 155 160 att Ile atg Met ttg Leu ccc Pro 220 gcg Ala ttg Leu aat tcc Ser gcc Ala tcc Ser 205 ggC Gly gga Gly gcg Ala gct cag Gin cag Gin 190 aaa Lys gtc Val gac Asp aca Thr gca Ala 270 gat Asp ggc Gly tta Leu 210 ggC Gly ctt Leu atc Ile ggt Gly cag Gin aac Asn 195 tcg Ser ggc Gly gca Ala gct Ala ttc Phe 275 PCTIBOOIOO923 cag caa 595 Gin Gin 165 gca gcg 643 Ala Ala 180 att ggc 691 Ile Gly acg ctc 739 Thr Leu acg gca 787 Thr Ala ttc cca 835 Phe Pro 245 gaa atg 883 Glu Met 260 ccc agg 931 Pro Arg Lys Asn Ala tca Ser tagtcgcgga aacggccctt aat <210> 984 <211> 278 <212> PRT <213> Corynebacterium glutaniicum <400> 984 met Thr Phe Gly Giu Lys Leu Leu Asn 1 Leu Cys Val Gly Ile Asp Pro His Giu 25 Leu Pro Val Asn Val Asp Gly Leu Ala 40 Glu Ala Phe Ala Asp Thr Val Ala Leu Tyr Glu Arg Phe Gly Ser Ala Gly Phe Gin Thr Leu Arg Giu Arg Gly Cys Leu Thr Thr Arg Gin Giu Asp Arg Ser Ala Val Glu Ala 1352 WO 01/00843 Gly Asp Ile Pro Ala Ser 115 Gly Phe His 130 Arg Gly Val 145 Leu Gin Asp Val Asp Gin Ala Giy Asn 195 Arg Leu Ser 210 Gin Gly Gly 225 His Leu Aia Asp Ilie Ala Pro Gly Phe 275 Thr Ser Asp Leu 150 Asn Ala Val Gly Ser 230 Asn Lys Ser Met Ser Pro 135 Ala Ala Leu Val1 Gly 215 Asp Val Asn Gly 105 Ala Phe Thr Gly Ala 185 Gly Ile Asp Arg Val 265 Trp 110 Pro Gin Ala Gin Gin 190 Lys Val Asp Thr Ala 270 PCTIIBOO/00923 Leu Asp Tyr Leu His Gly Arg Giu 160 Gin Ile 175 Gly Lys Pro Pro Gly Ala Met Ala 240 Gly Pro 255 Asp Phe <210> 985 <211> 852 <212> DNA <213> Corynebacterium giutamicum <22 0> <221> CDS <222> (101) (829) <223> RXN01892 <400> 985 ggtctcagtg gcttcttggt tgctgtgatt ttttcaaggc gtaccccgtg gccgatgtta aaagcggttg gcacaacccc tactgaagga gaacaccact gtg acc acc tcg agt 115 Val Thr Thr Ser Ser 1 gaa caa ccc cgt aca gga tac aag cga gtg atg tta aag ctc gga ggt 163 Glu Gin Pro Arg Thr Gly Tyr Lys Arg Vai Met Leu Lys Leu Giy Gly 15 gaa atg ttt ggt ggt ggc aaa gtc ggc gtc gat cct gat gta gta gac 211 Giu Met Phe Gly Gly Gly Lys Val Giy Val Asp Pro Asp Val Val Asp 30 1353 WO 01/00843 aat gtt gca Asn Val Ala PCT/EBOO/00923 cgt cag atc gct Arg Gin Ile Ala gtt gct aaa act Val Ala Lys Thr gca gag att Ala Glu Ile 259 gcc gtt Ala Val gtt atc ggt ggc Val Ilie Gly Gly aac ttc ttc cgc Asn Phe Phe Arg gct gag ctt cag Ala Glu Leu Gin cag cgt ggc atg gac cgc gca cgg tcc gat tac Gin Arg Gly Met Asp Arg Ala Arg Ser Asp Tyr 75 atg ggt atg ctc Met Gly Met Leu aca gtc atg aac Thr Val Met Asn ctc gcc ttg cag gac ttc ctc ggt cag Leu Ala Leu Gin Asp Phe Leu Giy Gin cat ggc His Gly 100 gtt gaa tgc Val Giu Cys cca tat ctg Pro Tyr Leu 120 cgt Arg 105* gtc cag acc gcc Val Gin Thr Ala atc Ile 110 aac atg gca cag gtc gca gaa Asn Met Ala Gin Val Ala Glu 115 cca ctg cgc gca Pro Leu Arg Ala gaa Giu 125 cgc cac ctg gaa Arg His Leu Giu ggc cgc gtt Gly Arg Val gtc atc Val Ile 135 ttc ggt gct ggc Phe Gly Ala Gly atg Met 140 ggt atg ccg tac ttt tcc acg gac acc Gly Met Pro Tyr Phe Ser Thr Asp Thr 145 act Thr 150 gct gca cag cgt Ala Ala Gin Arg ttg gaa atc ggc Leu Giu Ile Gly tgt Cys 160 gac gtc cta ctg Asp Val Leu Leu atg Met 165 gct aag gct gtt Ala Lys Ala Val ggt gtg tac agc gat gat cct cgt acc Gly Val Tyr Ser Asp Asp Pro Arg Thr 175 aac cca Asn Pro 180 gat gct gag ctc ttc acc gaa att Asp Ala Giu Leu Phe Thr Giu Ilie 185 act Thr 190 cca aag gaa gta Pro Lys Giu Val att gag aag Ile Giu Lys 195 atg gac aac Met Asp Asn ggc ctg aag Gly Leu Lys 200 gtt gcc gat gca Val Ala Asp Ala act Thr 205 gca ttc agc ctc Ala Phe Ser Leu tgc Cys 210 aag atg Lys Met 215 cct atc ttg gtg Pro Ile Leu Val ttt Phe 220 aac ctg ctt act Asn Leu Leu Thr gaa Giu 225 ggc aac att gct Giy Asn Ilie Ala cgc Arg 230 gcc atc agc ggt Ala Ilie Ser Gly cgt atc ggt act Arg Ilie Gly Thr ctg Leu 240 gtc gag tcc Val Glu Ser tgatacattt agtcttataa aca <210> 986 4211> 243 <212> PRT <213> Corynebacterium glutainicum 1354 WO 01/00843 PCTIiBOO/00923 <400> 986 Val Thr Thr Ser Ser Giu Gin Pro Arg Thr Gly Tyr Lys Arg Val Met 1 5 10 Leu Lys Leu Gly Gly Glu Met Phe Gly Gly Gly Lys Val Gly Val Asp 25 Pro Asp Val Vai Asp Asn Val Ala Arg Gin Ile Ala Giu Val Ala Lys 40 Thr Gly Ala Giu Ile Ala Val Val Ile Gly Giy Gly Asn Phe Phe Arg 55 Gly Ala Giu Leu Gin Gin Arg Gly Met Asp Arg Ala Arg Ser Asp Tyr 70 75 Met Gly Met Leu Gly Thr Val Met Asn Cys Leu Ala Leu Gin Asp Phe 90 Leu Gly Gin His Giy Val Giu Cys Arg Val Gin Thr Ala Ile Asn Met 100 105 110 Ala Gin Val Ala Glu Pro Tyr Leu Pro Leu Arg Ala Giu Arg His Leu 115 120 125 Giu Lys Giy Arg Vai Val Ile Phe Giy Ala Gly Met Gly Met Pro Tyr 130 135 140 Phe Ser Thr Asp Thr Thr Ala Ala Gin Arg Ala Leu Giu Ile Gly Cys 145 150 155 160 Asp Val Leu Leu Met Ala Lys Ala Val Asp Gly Val Tyr Ser Asp Asp 165 170 175 Pro Arg Thr Asn Pro Asp Ala Giu Leu Phe Thr Giu Ile Thr Pro Lys 180 185 190 Giu Val Ile Giu Lys Gly Leu Lys Val Ala Asp Ala Thr Ala Phe Ser 195 200 205 Leu Cys Met Asp Asn Lys Met Pro Ile Leu Val Phe Asn Leu Leu Thr 210 215 220 Giu Gly Asn Ile Ala Arg Ala Ile Ser Gly Giu Arg Ile Gly Thr Leu 225 230 235 240 Val Giu Ser <210> 987 <211> 798 <212> DNA <213> Corynebacteriun giutarnicum <22 0> <221> CDS <222> (47)..(775) <223> FRXA01892 <400> 987 1355 WO 01/00843 atgttaaaag cggttggcac aacccctact gaaggagaac PCT/IBOOIOO923 accacggtg acc acc tcg 58 Vai Thr Thr Ser agt Ser ggt Gly gac Asp at t Ile cag Gin ggc Gly ggc Giy gaa Giu gtt Val1 acc Thr atg Met 165 cca Pro aag Lys aac Asn gaa Giu gaa Giu aat Asn gcc Ala cag Gin aca Thr gt t Val cca Pro gtc Val act Thr 150 gc t Aia gat Asp ggc Giy aag Lys caa Gin atg Met gt t Val1 gtt Val cgt Arg gtc Val gaa Glu tat Tyr atc Ile 135 gc t Ala aag Lys gc t Ala ctg Leu atg Met 215 gga Gly ggc Gly atc Ile ggc Gly cgc Arg c tc Leu cag Gin cgc Arg ggc Gly gcg Aia 155 ggt Gly acc Thr gat Asp gtg Val1 aaa Lys gtc Val gaa Giu aac Asn cgg Arg ttg Leu gcc Al a gaa Giu 125 ggt Gly gaa Giu tac Tyr att Ile act Thr 205 aac Asn cga Arg ggc Giy gtt Val t tc Phe tcc Ser cag Gin atc Ile 110 cgc Arg atg Met atc Ile agc Ser act Thr 190 gca Ala ctg Leu gtg Val 15 gtc Vai gct Ala ttc Phe gat Asp gac Asp 95 aac Asn cac His ccg Pro ggc Gly gat Asp 175 cca Pro t tc Phe ctt Leu tta aag ctc gaa Lys gat Asp gga Gly gc t Ala ggt Gly ggt Giy cag Gin aag Lys 130 tcc Ser gtc Vai cgt Arg gta Val tgc Cys 210 ggc G ly Leu gta Val gca Alagag Giu atg Met cag Gin gtc Val 115 ggc Gly acg Thr cta Leu ac c Thr att Ile 195 atg Met aac Asn Giu gta Val gag Giu ctt Leu ctc Leu cat His 100 gca Ala cgc Arg gac Asp ctg Leu aac Asn 180 gag Giu gac Asp att Ile 106 154 202 250 298 346 394 442 490 538 586 634 682 730 gct cgc gcc atc agc ggt gaa cgt atc ggt act ctg gtc gag tcc 1356 WO 01/00843 PC Ala Arg Ala Ile Ser Gly Giu Arg Ile Gly Thr Leu Val Giu Ser 230 235 240 tgatacattt agtcttataa aca <210> 988 <211> 243 <212> PRT <213> Corynebacterium glutamicum <400> 988 Val Thr Thr Ser Ser Giu Gin Pro Arg 1 Leu Pro Thr Giy Met Leu Ala Glu Phe 145 Asp Pro Glu Leu Glu 225 Leu Val Ala G 1u Met Gin Val 115 Giy Thr Leu Thr Ile 195 Met Asn 5 Gly Asp Ile Gin Giy Giy Glu Vai Thr Met 165 Pro Lys Asn Ala Glu Asn Aia Gin 70 Thr Vai Pro Vai Thr 150 Al a Asp Gly Lys Arg 230 Met Val Vai Arg Vai Giu Tyr Ile 135 Ala Lys Ala Leu Met 215 Ala Phe Aia Val Gly Met Cys Leu 120 Phe Ala Al a Giu Lys 200 Pro Ile Gly Gly Ile Giy Arg 75 Leu Gin Arg Giy Ala 155 Gly Thr Asp Val Giu 235 Tyr Lys Ala Giy Al a Ala Thr Ala Met 140 Leu Val Glu Ala Phe 220 Arg Arg Gly Val1 Phe Ser Gin Ile 110 Arg Met Ile Ser Thr 190 Ala Leu Gly Val Val Ala Phe Asp Asp Asn His Pro Gly Asp 175 Pro Phe Leu Thr ~TfJIB00/00923 798 Met Asp Lys Arg Tyr Phe Met* Leu Tyr Cys 160 Asp Lys Ser Thr Leu 240 Val Glu Ser 1357 WO 01/00843 WO 0100843PCTIIBOO/00923 <210> 989 <211> 798 <212> DNA <213> Corynebacterium <220> <221> CDS <222> (775) <223> RXA00105 <400> 989 catcttgatc tgtcgccctg giutamicun aacttgcaga tgaacttctc gagatgtgcg cgaagttcta cgaggagaat ggaacttact aacgctgtta tgatgacggc atg act gtt cca Met Thr Val Pro 1 cct tat gaa gac Pro Tyr Giu Asp ctt cgg aag att Leu Arg Lys Ilie gaa gaa ggg tcc Giu Giu Gly Ser cac aag His Lys gac gac cgc Asp Asp Arg cgc ttt gat Arg Phe Asp ggc acc ggc act Gly Thr Gly Thr tct tta ttc gga Ser Leu Phe Gly caa caa atc Gin Gin Ile aag aag gtc Lys Lys Val ctc aat gaa ggt ttt ccc ctt ctg acc Leu Asn Giu Gly Phe Pro Leu Leu Thr cat ttc His Phe cac tct gtt gtg His Ser Val Val gag ctt ttg tgg Giu Leu Leu Trp ctt cag ggg gat Leu Gin Gly Asp aac gtc aaa tgg Asn Val Lys Trp ctg Leu cag gat aac aac Gin Asp Asn Asn cgc att tgg aat Arg Ile Trp Asn tgg gca gat gag Trp Ala Asp Glu ggc gag ctg ggc Giy Glu Leu Giy gtt tat ggt gtc Val Tyr Giy Val cag tgg Gin Trp 100 cgt tct tgg Arg Ser Trp gct tta gaa Ala Leu Glu 120 acc cct gat ggt Thr Pro Asp Giy cac att gac cag His Ile Asp Gin atc tca. ggt Ile Ser Giy 115 aat att gtc Asn Ile Val act ctg cga aac Thr Leu Arg Asn cct gat tca cgt Pro Asp Ser Arg tcg gcg Ser Ala 135 tgg aat gtt tcc Trp, Asn Vai Ser ctt gaa aac atg Leu Giu Asn Met ctt ccc cct tgt Leu Pro Pro Cys ttg ctt ttc cag Leu Leu Phe Gin ctc Leu 155 tat gtc gcc gat Tyr Val Ala Asp aaa ctg tct tgc Lys Leu Ser Cys ctc tac cag cgt Leu Tyr Gin Arg tct Ser 170 gcg gac atg ttc Ala Asp Met Phe ggt gtg cct ttc Gly Val Pro Phe aac atc Asn Ile 180 1358 WO 01/00843 PCT/IBOO/00923 gca tct tat gca ctg ctc acc cac atg ttt gcc cag cag gca ggc ttg 691 Ala Ser Tyr Ala Leu Leu Thr His Met Phe Ala Gin Gin Ala Gly Leu 185 190 195 gaa gtc ggc gag ttc att tgg act ggc ggc gac tgc cac att tat gac 739 Giu Val Gly Glu Phe Ilie Trp Thr Gly Gly Asp Cys His Ile Tyr Asp 200 205 210 aac cac aag gaa cag gtc gcg gag cag ctg agc cga taagctcgcc 785 Asn His Lys Glu Gin Val Ala Glu Gin Leu Ser Arg 215 220 225 cctaccccac ctt 798 <210> 990 4211> 225 <212> PRT <213> Corynebacterium giutamicun <400> 990 Met Thr Val Pro Thr Pro Tyr Giu Asp 1 Glu Phe Thr Phe Arg Tyr Asp Arg Ala 145 Lys Val Gin 5 Lys Ilie Val1 Asp Glu Trp Gly Val Cys Gin 165 Ile Leu Leu Gly Asn Val Trp Asp Thr Leu Val Gin Ser 170 Leu Phe Arg Gly Gly Gly Gin Giu Asp Asn Giu 140 Tyr Asp Thr Trp Lys Thr Phe Glu Asp Leu Gly Asn 125 Leu Val Met His Thr 205 Ile Thr Pro Leu Asn Gly Arg 110 Pro Glu Ala Phe Met 190 Gly Cys His Ilie Tyr Asp Asn His Lys Glu Gin Val Ala Glu Gin Leu Ser 1359 WO 01/00843 WO 0100843PCTIIBOO/00923 Arg 225 <210> <211> <212> <213> <1'20> <221> <222> <223> 991 732

DNA

Corynebacteriun giutamicun

CDS

(101) (709) RXA0 0131 <400> 991 aggctctcgg cggtcagctc accgaactga ccaaggagca ggctgagtac atcggcgttg acgttgcagg cccattcaag ccggagcact accgctacta atg att gtc agc att Met Ile Val Ser Ile gag gga atc gac Glu Gly Ilie Asp ggc Gly gcc ggc aaa aac Ala Gly Lys Asn ctg gtt tcg gca Leu Val Ser Ala tta acg Leu Thr cag gtt att Gin Val Ile gca aaa gtc ctt Ala Lys Val Leu ttc cca cgt tat Phe Pro Arg Tyr gaa acc tcg Glu Thr Ser atg ggc gac Met Gly Asp att cac gcc caa ttg gcc gcg Ile His Ala Gin Leu Ala Ala ctc acc gac agc gcc tac gcc Leu Thr Asp Ser Ala Tyr Ala gca ctc cac ggc Ala Leu His Gly cgc Arg atg gcc acg ctt Met Ala Thr Leu gcc ctc gac Ala Leu Asp c ac His ttc gcg att gat Phe Ala Ile Asp gac Asp tta aat gcg ccc Leu Asn Ala Pro gtg gtg ctg ctc Val Val Leu Leu cga tac gtc gcc Arg Tyr Val Ala aac gcg gct tat Asn Ala Ala Tyr gcc gcc aga ttg Ala Ala Arg Leu ctt gac Leu Asp 100 gac gac gcc Asp Asp Ala ctc cca cgt Leu Pro Arg 120 cgc tgg gtt gcc Arg Trp Val Ala ctg gaa ttc ggg Leu Glu Phe Giy cgg ctt ggg Arg Leu Gly 115 gcg gag gta Ala Giu Val ccg acg ctt caa gtg ttg ttg gat acc Pro Thr Leu Gin Val Leu Leu Asp Thr 125 gcg caa gat agg gct aga cgt cga gaa gcg ctt gac Ala Gin Asp Arg Ala Arg Arg Arg Glu Ala Leu Asp 135 140 145 tcc gcg cgt gcg Ser Ala Arg Ala 547 595 cgg gac cgc tat gaa tcg gat tcg gcg ctg cag caa cgc acc gcc gag Arg Asp Arg Tyr Glu Ser Asp Ser Ala Leu Gin Gin Arg Thr Ala Glu 1360 WO 01/00843 150 cac tat cgc His Tyr Arg gtt gcc cct Val Ala Pro ttc ctg ggt Phe Leu Gly 200 cgc Arg gat Asp 185 act Thr ctc Leu 170 gaa Glu ata Ile PCTIBOO/00923 160 165 gcg gac aac tgg gaa tca ccg tgg atc gtg 643 Ala Asp Asn Trp Giu Ser Pro Trp Ile Val 175 180 ccc ggc cac gtt gcg cag aga atc gtg gaa 691 Pro Gly His Vai Ala Gin Arg Ile Val Glu 190 195 taatcccaat tagcaggaag gat 732 <210> 992 <211> 203 <212> PRT <213> Corynebacteriun giutamicum <400> 992 Met Ile Val Ser Ile Giu Gly Ile Asp 1 Vali Arg Gly Phe Vai Ala Phe Thr Asp 145 Gin Ser Gin 5 Thr Ser Asp Arg Asp Asp Gly Vai Ala Giu 165 Val Glu Gin Ile Leu His 70 Arg Asp Leu Ala Arg 150 His Val Phe Vai His Thr Phe Tyr Asp Pro Gin 135 Asp Tyr Ala Leu Ile Ala Asp Ala Val1 Ala Arg 120 Asp Arg Arg Pro Giy 200 Ala Lys Ala Tyr Asp 75 Asn Trp Leu Arg Ser 155 Ala Asp Asn Gly Val1 Ala Ala Leu Ala Val1 Gin Arg 140 Asp Ala Pro Lys Leu Glu Asn Ala Al a Vai 125 Arg Ser Asp Gly Asn Ala Ala Ala Ala Tyr Asp 110 Leu Glu Ala Asn His 190 Thr Phe Leu Thr Pro Thr Leu Leu Ala Leu Trp 175 Val Leu Pro His Leu Gly Ala Glu Asp Leu Gin 160 Giu Ala 1361 WO 01/00843 WO 0100843PCTIBOO/00923 <210> 993 <211> 531 <212> DNA <213> Corynebacterium glutanicun <220> <221> <222> <223>

CDS

(101)..(508) RXA0 0266 <400> 993 agtaggggat cgtccagcga agcggtcgca gaggctgtga ggaagctcta agtcgactta agtgcgcgaa gcagaccacc attaggtaga atcacccaac atg act gaa cgt act Met Thr Giu Arg Thr ctc atc ctt atc Leu Ile Leu Ile cca gac ggt gtt Pro Asp Gly Val aac gga cac gtc Asn Gly His Val ggc gaa Gly Glu atc atc gca Ile Ile Ala ctg cgt gtt Leu Arg Val att gag cgc aag Ile Glu Arg Lys ctg aag ctc gct Leu Lys Leu Ala gct ctg gat Ala Leu Asp gaa gag cac Giu Giu His gca gac cgc gag Ala Asp Arg Glu acc Thr gct gaa aag cac Ala Giu Lys His gct gac Ala Asp aag cca ttc ttc Lys Pro Phe Phe gag ctc gtt gaa GluLeu Val Giu atc acc tct gca Iie-Thr Ser Ala cct Pro ctg atc gca ggc Leu Ile Ala Gly gtc gaa ggc gag Val Glu Gly Glu gca atc gat gca Ala Ile Asp Ala tgg Trp 307 355 403 cgt cag ctt gct Arg Gin Leu Ala ggc acc gac cca Gly Thr Asp Pro gct aag gca acc Ala Lys Ala Thr cca ggc Pro Gly 100 acc atc cgc Thr Ile Arg ggt tct gat dly Ser Asp 120 9gc Gly 105 gat ttc gca ctg Asp Phe Ala Leu act Thr 110 gtt gga gag aac Val Gly Glu Asn gtt gtt cac Val Val His 115 atc tgg ttc Ile Trp, Phe tcc cca gag tcc Ser Pro Giu Ser gct gag Ala Giu 125 cgc gag atc Arg Giu Ilie cct aac ctg taatttttac ggttagaaaa aaa Pro Asn Leu 135 <210> 994 <211> 136 <212> PRT <213> Corynebacterium glutamicum <400> 994 Met Thr Giu Arg Thr Leu Ile Leu Ile Lys Pro Asp Gly Val Thr Asn 1362 WO 01/00843 Gly His Val Gly Giu Ile Ile Ala Arg Ile Glu 25 Leu Ala Ala Leu Asp Leu Arg Val Ala Asp Arg His Tyr Glu Glu His Ala Asp Lys Pro Phe Phe Phe Ile Thr Ser Ala Pro Leu Ile Ala Gly Ile 70 75 Ala Ile ASP Ala Trp, Arg Gin Leu Ala Gly Gly 90 Lys Ala Thr Pro Gly Thr Ile Arg Gly Asp Phe 100 105 Giu Asn Val Val His Gly Ser Asp Ser Pro Giu 115 120 Ilie Ser Ilie Trp Phe Pro Asn Leu 130 135 <210> 995 <211> 831 <212> DNA <213> Corynebacterium giutamicun <22 0> <221> CDS <222> (101)..(808) <223> RXA00718 <400> 995 gcaccaagct gcacaccgtt cagcttggtg atcagaagcc actcctctga gctgaccagc ttatacaagg tggtccaact Gly Ala Leu Gly Pro Thr 110 Glu PCTIIBOO/00923 Leu LYS Giu Lys Val Glu Giu Arg Val Ala Val Gly Arg Glu aggttccctt cgtgcactga gtg acg gaa att tcc aac atg cct Asn Met Pro acc gga Thr Gly aag tac Lys Tyr ctt aac Leu Asn acc gct gcc Ala tcc Ser ga t Asp ggg Gly t tg ggt ggc Gly Gly acc aca Thr Thr act ggt Thr Gly att gac Ilie Asp ccg ttg gta gcc Val Ala 15 gcg ctc Ala Leu 30 tac cgc Tyr Arg gat agc Asp Ser tct gac Val Thr Giu Ile Ser 1 atc gac ggg ccg tct ggc Ile Asp Gly Pro Ser Gly gca acc cgt ctc tcg gcc Ala Thr Arg Leu Ser Ala gtc gca acg ctt cat gtg Val Ala Thr Leu His Val gca gcc gtg atc gct gca Ala Ala Val Ile Ala Ala gat ccc gcc tcc act gag 1363 WO 01/00843 Thr Ala Val Leu Pro Leu Ser Ile Ser Asp 75 gtg Val gtc Val aac Asn gtC Val atc Ile 150 ttt Phe ctg Leu tca Ser atg Met ggC Gly gt C Val PCTIIBOO/00923 Asp Pro Ala Ser Thr Giu 80 gac atc cgc gga cca gaa 403 Asp Ile Arg Gly Pro Glu 100 atc cct gag gtt cgt gaa 451 Ile Pro Giu Val Arg Giu 115 gcc aaa gca cat cgc tgc 499 Ala Lys Ala His Arg Cys 130 gtg ctt gtc gac gcg ccc 547 Val Leu Val Asp Ala Pro aag gcg Lys Ala gac caa Asp Gin gca gat Ala Asp ccg ctg Pro Leu 200 acc atg Thr Met 215 ttg cag Leu Gin aga gac Arg Asp ctc acc Leu Thr 155 acc gca Thr Ala i7 0 gtt cgc Val Arg cca gca Pro Aia caa gta Gin Val gcc Ala ttC Phe acc Thr gac Asp 210 cta cag cga Gin Arg gac gct Asp Ala 180 cgt gcc Arg Ala 195 acc tct Thr Ser gtg gaa Val Giu ctc atc cac Asp His Leu Ile His Leu 225 tcc gct gaa agg agc aac cag tgactgataa acacaccatg cct Ser Ala Glu Arg Ser Asn Gin 230 235 <210> 996 <211> 236 <212> PRT <213> Corynebacteriun giutainicum <400> 996 Val Thr Giu Ile Ser Asn Met Pro Ala Gly Gly Leu Ile Val Ala Ile 1 5 10 Asp Gly Pro Ser Gly Thr Gly Lys Ser Thr Thr Ser Arg Ala Leu Ala 25 Thr Arg Leu Ser Ala Lys Tyr Leu Asp Thr Gly Ala Met Tyr Arg Val 40 Ala Thr Leu His Val Leu Asn Gin Gly Ile Asp Pro Ala Asp Ser Ala 55 s0 1364 WO 01/00843 WO 0100843PCT/IBOO/00923 Ala Val Ilie Ala Ala Thr Ala Val Leu Pro Leu 70 Pro Ala Ser Thr Glu Val Leu Leu Ala Gly Val Ile Arg Gly Pro Giu Val Thr Gin Asn Val Ser 100 105 Pro Glu Val Arg Glu Asn Leu Val Ala Leu Gin 115 120 Lys Ala His Arg Cys Val Val Giu Gly Arg Asp 130 135 Leu Val Asp Ala Pro Ile Lys Ala Phe Leu Thr 145 150 155 Arg Ala Gin Arg Arg Phe Asp Gin Asp Thr Ala 165 170 Asp Phe Asp Ala Val Leu Ala Asp Val Val Arg 180 185 Ser Thr Arg Ala Ala Ser Pro Leu Lys Pro Ala 195 200 Val Asp Thr Ser Asp Met Thr Met Asp Gin Val 210 215 His Leu Val Giu Ala Ser Ala Giu Arg Ser Asn 225 230 235 <210> 997 <211> 1785 <212> DNA <213> Corynebacierium giutanicun <2 <221> CDS <222> (1762) <223> RXA01599 <400> 997 caccgaacgt iiaagitttc cgcaccgcct gacicgaaaa tcgatgtita ggttcaacca ggaaggtcgt ctcagacatc aaa gtc cgt ccc acc aaa cac at Lys Val Arg Pro Thr Lys His Ilie ica cic ggc aaa ggc ctg acc gca Ser Leu Gly Lys Gly Leu Thr Ala gca cgg gga ctg tcg gig acc atg tic gtc acc Phe Val Thr 15 gca agc ctt Ala Ser Leu 30 cag aag ctg gatctttit aaagagctgt aig acc ict agi cga Met Thr Ser Ser Arg 1 ggt gga gti git icc Gly Gly Val Val Ser ggi caa ttg ctg ati Gly Gin Leu Leu Ile gat cca tac cic aat 1365 WO 01/00843 Ala Arg Gly gtt gat ccg Vai ASP Pro acc gaa gac Thr Giu ASP ttc ctc gat Phe Leu Asp gtg tat tcc Val Tyr Ser aaa act gtg Lys Thr Val 120 att ttg agc Ile Leu Ser 135 gtg atc tct Val Ile Ser 150 ttc ctt gaa Phe Leu Giu tgc ttc ttc Cys Phe Phe gag ctg aag Glu Leu Lys 200 atc ggt att Ile Giy Ile 215 cct *caa ggt Pro Gin Gly 230 gaa ggc gtt Giu Giy Val gat-gtc ctc Asp Val Leu ggC ctt ccg Val atg Met gaa Giu 75 ctg Leu atc Ile atc Ile gaa Glu ggt Giy 155 cgc Arg tgt Cys ccc Pro gat Asp gat Asp 235 tgc Cys gag Glu gat Thr aat Asn aca Thr ggg Gly gcc Ala cca Pro cca Pro 140 ggc Gly cag Gin tct Ser acc Thr gct Ala 220 aag Lys cct Pro cac His gtt Met 45 cc t Pro gac Asp c tc Leu aag Lys cac His 125 gat Asp acc Thr gta Val ttg Leu cag Gin 205 ctc Leu atc Ile gat Asp ctg Leu gac Lys gaa Giu gat Asp gcc Ala cgc Arg act Thr cat His ggt Gly cat His 175 cca Pro tc t Ser ctt Leu atg Met agt Ser 255 ac c Thr agc Leu cac His ttg Leu 80 aat Asn agg Arg gat Asp ggt G ly gac Asp 160 gaa Glu tac Tyr gtc Val cgt Arg atg Met 240 tc t Ser t tc Phe acc Pro gaa Glu cac His acc Thr gag Giu atc Ile 130 gcc Al a gaa Glu ggt Gly gct Ala gag Giu 210 gat

ASP

gat Asp tac Tyr att Ile c ac Tyr gtc Val tac Tyr acc Thr tac Tyr 115 aaa Lys cca Pro tcc Ser cgt Arg acc Thr 195 ctg Leu c9g Arg gtt Val1 aac Asn cgt Arg 275 gat PCTIBOO/00923 Leu Asn ttt gtc 307 Phe Val gag cgt 355 Glu Arg ggc aag 403 Giy Lys 100 ctg ggt 451 Leu Gly gct cgt 499 Ala Arg gac gtg 547 Asp Val cag cca 595 Gin Pro 165 gaa aac 643 Giu Asn 180 tca ggt 691 Ser Giy cgc ggc 739 Arg Gly gag gtc 787 Glu Val gat tat 835 Asp Tyr 245 att cca 883 Ile Pro 260 cgc ctg 931 Arg Leu ctg ctg 979 Gly Leu Pro Phe Arg Asp Val Asp Trp Ser Thr Trp His Asp Leu Leu 1366 WO 01/00843 WO 0100843PCT/IB00100923 gaa cgg 1027 Glu Arg 295 aag tac 1075 Lys Tyr 310 cgc gct 1123 Arg Ala acc tca 1171 Thr Ser ggt ttg 1219 Gly Leu gaa ggc 1267 Giu Gly 375 ctt ctt 1315 Leu Leu 390 cgc cag 1363 Arg Gin gca acg 1411 Ala Thr tcg ggt 1459 Ser Gly gca acc 1507 Ala Thr 455 gaa gtc 1555 Giu Val 470 280 gtg aac aac cca cgc Val Asn Asn Pro Arg 300 att gat ctt ccc gat Ile Asp Leu Pro Asp 315* gca ggc tac gcc aat Ala Gly Tyr Ala Asn 330 gat gat tgc gaa acc Asp Asp Cys Glu Thr 285 cat His gag ctc acc Glu Leu Thr gct tat ctc tca Ala Tyr Leu Ser 320 290 gtc ggc atc gtg ggc Val Gly Ile Val Gly 305 gtg gtg gaa gct gtt Val Val Glu Ala Val 325 aat atc aag tgg att Asn Ile Lys Trp Ile 340 atg aaa gcg ctc agc Met Lys Ala Leu Ser tgg acg Trp Thr cca tct Pro Ser 350 cgc Arg 335 acc Thr ggc gcc Gly Ala 345 gat gcc ASP Ala atc gtg gtt ccc Ile Val Val Pro 365 ggt gcg att acg Gly Ala Ilie Thr gga Gly ttt Phe 355 ggt ttc ggt atc cga ggt atc Gly Phe Gly Ile Arg Gly Ilie 370 gca cgt gag cac aag atc ccg Ala Arg Glu His Lys Ile Pro atc Ile 380 ggc cta tgc ctt Gly Leu Cys Leu 395 gca gga ctg gag Ala Gly Leu Giu .410 ggc Gly ctg cag tgc acc Leu Gin Cys Thr 38.5 gtc atc gag gqca gca Val Ile Giu Ala Ala 405 gag ttt gac cca gct Giu Phe Asp Pro Ala 420 cag gca tca tcc Gin Ala Ser Ser 415 cag cca Gin Pro 425 gaa gct Giu Ala gtg atc gcc acc atg Val Ilie Ala Thr Met 430 gat ctg ggt ggc acc Asp Leu Gly Gly Thr 445 gaa ggc tcc tta gta Giu Gly Ser Leu Val gaa gag cag aaa gct gct gtg Giu Giu Gin Lys Ala Ala Val 435 atg cgt ctt ggc gca tat cct Met Arg Leu Gly Ala Tyr Pro 450 gcg gaa ctg tat ggc aca acg Ala Glu Leu Tyr Gly Thr Thr 440 ctg gag Leu Giu 460 tcc gag cgc cac cgt Ser Giu Arg His Arg 475 465 cac cgc tat gag gtc His Arg Tyr Giu Val 480 aat aat gcc tac Asn Asn Ala Tyr 485 1367 WO 01/00843 WO 0100843PCTIiBOOIOO923 cgc gcc 1603 Arg Ala cag att gct Gin Ile Ala 490 gaa ggt tca Glu Gly Ser2 cct gat gga cat ttg gtg gag ttc 1651 Pro Asp Gly His Leu Val Giu Phe 505 cct tat ctg gtg gca acc cag gcg 1699 Pro Tyr Leu Val Ala Thr Gin Ala 520 525 acc cat gct cat cca ctg ttt tac 1747 Thr His Ala His Pro Leu Phe Tyr 535 540 ctg cgt gtc cac cct tagatctaca al 1785 Leu Arg Val His Pro 550 <210> 998 <211> 554 <212> PRT <213> Corynebacterium glutainicum <400> 998 ksp Leu Val Phe 495 Itg gag tac ccc lai Glu Tyr Pro 510 :ac cct gag tac iis Pro Giu Tyr ;gc ctg gtg aag 1ly Leu Val Lys 545 :gtgatcat ggt tcc gga Ser Gly aaa gag Lys Giu 515 aaa tct Lys Ser 530 acc gct Thr Ala ac c Thr 500 gtg Val1 cgt Arg ttg Leu Val Ser Lys Glu Asp Ala Arg Thr His Gly tca Ser cat His cca Pro gag Giu Thz.

Leu Leu His Leu Asn Arg Asp Gly Asp Met Thr Ser Ser Gly Gly Val Val Gl.y Gin Leu Leu Asp Pro Tyr Leu Gly Giu Val Phe Gly His Tyr Giu Val Thr Thr Gly 100 Gly Giu Tyr Leu 115 Glu Ile Lys Ala 130 Asn Ala Pro Asp Arg Ser Ile Asn Val Arg Lys Gly Arg Val1 Lys Val Arg Pro Leu Arg Asp 55 Glu Leu Tyr Thr Leu 135 Ile Gly Gly 40 Pro Asp Asp Ser Val 120 Ser Ser Thr 10 Gly Ser Thr Al a Asn 90 Val Val1 Gly Val His Thr Thr Asn Thr Gly Ala Pro Pro 140 Gly Ile Ala Met Pro Asp Leu Lys His 125 Asp Thr Phe Ala Gin Phe Leu Asn Glu 110 Ile Ala Val 1368 WO 01/00843 145 Ile Giu Se Ile Gly Ar Leu Ala Th 19 Ala Giu Le 210 CYS ASP Ar 225 Cys ASP Va Ile Tyr As Ile Ile Ar 27 Trp His As 290 Val Gly Ii 305 Val Val Gi Asn Ile Ly Met Lys Al Gly Ilie Ar 370 Giu His Ly 385 Vai Ile Gi Giu Phe As Gin Lys Al 43 Leu Gly Al 450 Leu Tyr Gi Gin Giu 180 Ser Arg Giu Asp Ile 260 Arg Leu Val Ala Trp 340 Leu Gly Ilie Ala Pro 420 Ala Tyr Thr Pro 165 Asn Gly Gly Val Tyr 245 Pro Leu Leu Gly Val 325 Ile Ser Ilie Pro Ala 405 Ala Val Pro Thr 150 Phe Cys Giu Ile Pro 230 Giu Asp Gly Glu Lys 310 Arg Thr Giy Glu Leu 390 Arg Ala Ser Ala Giu 470 Glu Phe Lys 200 Ile Gly Val1 Leu Pro 280 Val Ilie Ala Asp Asp 360 Lys Gly Ala Gin Glu 440 Leu Ser Ala Ile 185 Thr Leu Leu Val Tyr 265 Phe Asn Asp Gly Asp 345 Ala Ile Leu Gly Pro 425 Ala Glu Glu Ala 170 His Lys Pro Lys Ser 250 Arg Arg Asn Leu Tyr 330 Cys Ile Gly Cys Leu 410 Val1

ASP

Glu Arg 155 Arg Cys Pro Asp Asp 235 Cys Giu Asp Pro Pro 315 Ala Glu Val Ala Leu 395 Giu Ile Leu Gly His 475 Gin Ser Thr Ala 220 Lys Pro His Val1 Arg 300 Asp Asn Thr Val Ile 380 Gly Gin Ala Gly Ser 460 Arg Val Leu Gin 205 Leu Ile Asp Leu Asp 285 His Ala Trp Pro Pro 365 Thr Leu Ala Thr Gly 445 Leu His PCT/1BOO/00923 160 His Giu 175 Pro Tyr Ser Val Leu Arg met Met 240 Ser Ser 255 Thr Phe Ser Thr Leu Thr Leu Ser 320 Arg Thr 335 Gly Ala Gly Phe Ala Arg Cys Thr 400 Ser Thr 415* Giu Giu Met Arg Ala Glu Tyr Glu 1369 WO 01/00843 WO 0100843PCT/EBOO/00923 Val Asn Asn Ala Tyr Arg Ala Gin Ile Ala Giu 485 490 Phe Ser Gly Thr Ser Pro Asp Gly His Leu Val 500 505 Pro Lys Glu Val His Pro Tyr Leu Val Ala Thr 515 520 Tyr Lys Ser Arg Pro Thr His Ala His Pro Leu 530 535 Lys Thr Ala Leu Giu Leu Arg Val His Pro 545 550 <210> 999 <211> 3462 <212> DNA <213> Corynebacterium glutanicui <22 0> <221> CDS <222> (101)..(3439) <223> RXN02234 <400> 999 acccagaggc cgctgccggc ccaaatgatg caagccccct Gly Ser Asp Leu Val 495 Giu Phe Val Glu Tyr 510 Gin Ala His Pro Giu 525 Phe Tyr Gly Leu Val 540 gtttgaccag tttgttgagc tgatggatgc agacgctcag aagaaaggcg cataaataac atg cca aag cgt tca Met Pro Lys Arg Ser gat Asp cag Gin aag Lys atc Ile gag Glu cac His aac Asn cac His gaa Giu gga Gly gac Asp tac Tyr gac Asp at c Ilie 105 gtc Val ttc Phe ctg Leu cca Pro atc Ile gcc Ala Cag Gin ggt Gly ggc Gly 30 cc Leu gac Asp ttc Phe acc Thr ctc Leu 110 gtc Val1 cgc Arg cca Pro gag Giu gag Giu act Thr at Ile gtg Val1 gca Ala cca Pro cag Gin gca Ala 100 163 211 259 307 355 403 451 ggc atc ctg gaa aag tac ggc Gly Ile Leu Giu Lys Tyr Gly 115 1370 WO 01/00843 gtt gaa ctc Val Giu Leu 120 PCT/iBOO/00923 atc ggt gca gac Ile Gly Ala Asp gat gcc att gag cgc ggc gaa gat Asp Ala Ile Giu Arg Giy Giu Asp 130 cgc cag Arg Gin 135 aag ttc aag gat Lys Phe Lys Asp gtc acc acc atc Val Thr Thr Ile ggt Giy 145 ggc gaa tcc gcg Giy Giu Ser Ala cgt Arg 150 tcc cgc gtc tgc Ser Arg Val Cys aac atg gaa gaa Asn met Giu Glu gtc Val 160 cac gag act gtc His Giu Thr Val gaa ctc ggc ctt Giu Leu Gly Leu gta gtc gtg cgt Val Val Val Arg tcc ttc act atg Ser Phe Thr Met ggt ggc Gly Giy 180 ctg ggC tcc Leu Giy Ser gg t Giy 185 ctt gca tac aac Leu Aia Tyr Asn gaa gac ctt gag Giu Asp Leu Glu cgc atc gct Arg Ile Ala 195 atc gaa gaa Ile Giu Glu ggt ggc gga ctt gct gca tct cct Gly Giy Gly Leu Ala Ala Ser Pro 200 205 gaa gca aac gtc Giu Ala Asn Val ttg Leu 210 tcc atc Ser Ile 215 ctt ggt tgg aag Leu Giy Trp Lys ttc gag ctc gag Phe Giu Leu Giu ctc Leu 225 atg cgc gat acc Met Arg Asp Thr gac aac gtt gtg Asp Asn Val Vai atc tgc tcc att le'Cys Ser Ile aac gtc gac gca Asn Vai Asp Aia ggc gtg cac acc Giy Val His Thr ggC Gly 250 gac tct gtc acc Asp Ser Vai Thr gca cct gcc ctg Ala Pro Ala Leu acc ctg Thr Leu 260 act gac cgt Thr Asp Arg cgc gag gtc Arg Giu Vai 280 gaa Giu 265 ttc cag aag atg Phe Gin Lys Met gat cag ggt atc Asp Gin Gly Ile gcc atc atc Ala Ile Ile 275 ttc gcc atc Phe Ala Ile ggc gtg gac acc Gly Vai Asp Thr gga tgt aac atc Gly Cys Asn Ile cag Gin 290 aac cca gtt gat ggc cgc atc atc acc att gag atg aac cca cgt gtg 1027 Asn Pro 295 tct cgt 1075 Ser Arg 310 aag atg 1123 Lys Met Val Asp Gly Arg Ile Thr Ile Giu Met 305 Asn Pro Arg Val tcc tcc gct ctg gca tcc aag gca acg ggc ttc cca att gcc Ser Ser Ala Leu Aia Ser Lys Ala Gly Phe Pro Ile gct gcc aag ctg gct atc gga tac acc ctg gat gag atc acc Ala Ala Lys 330 Leu Ala Ile Gly Thr Leu Asp Giu Ile Thr 340 1371 WO 01/00843 WO 0100843PCTIIBOO/00923 aac gac 1171 Asn Asp tao gtc 1219 Tyr Val1 got gat 1267 Ala Asp 375 too otg 1315 Ser Leu 390 otg gaa 1363 Leu Glu gca ggg 1411 Ala Gly ogo ooa 1459 Arg Pro ggo goa 1507 Gly Ala 455 tto oto 1555 Phe Leu 470 gao goa 1603 Asp Ala ggt otg 1651 Gly Leu gaa gao 1699 Glu Asp ato aot Ile Thr 345 gtg gto Val Val ggt gaa aoo oca got Gly Giu Thr Pro Ala 350 aag goc ooa ogo ttt Lys Ala Pro Arg Phe 365 ttg aoo aoo aoo atg Leu Thr Thr Thr Met gog ttt gag coo aco ato gao Ala Phe Giu Pro Thr Ile Asp 355 got tto gag aag ttt gto ggo Ala Phe Glu Lys Phe Val Gly 370 aoct Thr aag too gto Lys Ser Val ggt Gly gag gtc atg Giu Val Met 380 ggo ogo aao tao Gly Arg Asn Tyr 395 aco aag cag cag Thr Lys Gin Gin 410 att Ile ggt Gly goa goa otg aao Ala Ala Leu Asn 400 tto tgg aoo aag Phe Trp Thr Lys 385 aag gca otg ogt too Lys Ala Leu Arg Ser 405 oct gat gag tto tto Pro Asp Giu Phe Phe 420 ctg gaa gat otc aag Leu Giu Asp Leu Lys 415 gag cgc Glu Arg 425 aoo gaa Thr Glu gct aco gat aag goa Ala Thr Asp Lys Ala 430 ggo ogo oto tao gao Gly Arg Leu Tyr Asp 445 gaa gaa oto tao gaa Giu Glu Leu Tyr Glu got gtt Ala Val 43.5 gtg Val1 gtt gag Val Glu goa tot Ala Ser 460 goo gag ott gaa Ala Giu Leu Giu 475 ooa tto ota aao Pro Phe Leu Asn 490 got oto gtg oag tto Ala Leu Val Gin Phe 480 gaa gat oto otg ogo Glu Asp Leu Leu Arg 495 ato goa goo ott ogo Ile Ala Ala Leu Arg otg goa atg ogo ott Leu Ala Met Arg Leu 450 tot att gat oct tgg Ser Ile Asp Pro Trp, 465 ogo oag aag oto gtt Arg Gin Lys Leu Val 485 gaa goa aag tto atg Glu Ala Lys Phe Met 500 ooa gag tto got ggc Pro Glu Phe Ala Gly 515 ggo ato ogo ooa gta Gly Ile Arg Pro Val 530 too gao Ser Asp 505 ggo gta Gly Val 520 otg Leu oag Gin ogo aoc ttg ogt Arg Thr Leu Arg 525 510 o tg Leu too ota Ser Leu 1372 WO 01/00843 WO 0100843PCT/IBOO/00923 ttc aag 1747 Phe Lys 535 tac cac 1795 Tyr His 550 gca cca 1843 Ala Pro aac cgc 1891 Asn Arg gct ctt 1939 Ala Leu aac cca 1987 Asn Pro 615 ttc gag 2035 Phe Giu 630 gcg cag 2083 Ala Gin act cct 2131 Thr Pro att ggt 2179 Ile Gly ttc ggt 2227 Phe Gly 695 acc gca 2275 Thr Ala 710 act gtg gat acc tgt Thr Val Asp Thr Cys 540 tac tcc gca tac gag Tyr Ser Ala Tyr Giu 555 cag act gag cgt gaa Gin Thr Giu Arg Glu 570 atc ggc cag ggc atc Ile Gly Gin Gly Ile gca gca gag ttt gaa gct aag act ccg Ala Ala Giu Phe Giu Ala Lys Thr Pro 545 ctg gat cca gca gct gag tct gag gtc Leu Asp Pro Ala Ala Giu Ser Giu Val 560 565 aag gtc ctg Lys Vai Leu 575 ttg ggc tcc ggt cca Leu Giy Ser Gly Pro 580 gag ttc Giu Phe 585 gag ctc Giu Leu 590 tcc cgc gtc ggc tac Ser Arg Val Giy Tyr 605 gac tac tcc tgt gtt cac gca Asp Tyr Ser Cys Vai His Ala 595 gaa act gtc atg gtc aac tgc Giu Thr Val Met Val Asn Cys 610 gac acc gct gac cgc ctg tac Asp Thr Ala Asp Arg Leu Tyr acc Thr gtg tcc acc Vai Ser Thr gac tac Asp Tyr 620 cca ctg acc ttc Pro Leu Thr Phe 635 tcc ggc acc gtc Ser Gly Thr Val 650 gaa Giu gc a Ala gac gtc atg gag Asp Val Met Glu 640 ggt gtt atc gtc Giv Val Ile Val 625 gtc tac cac gct gag Val Tyr His Ala Giu 645 cag ctt ggt ggc cag Gin Leu Giy Gly Gin 660 655 ctg ggc Leu Gly 665 acc tcc Thr Ser ttg gca gat cgt Leu Ala Asp Arg cca gag gca atc Pro Giu Aia Ilie 685 ctg aac cgc gag Leu Asn Ara Giu ttg aag aag gct ggc gtc cct gtc Leu Lys Lys Ala Giy Vai Pro Vai 670 675 gac atg gct gag gac cgt ggc gag Asp Met Ala Giu Asp Arg Gly Giu 690 cag ctt cct gct cca gca ttc ggc Gin Leu Pro Aia Pro Ala Phe Gly 705 c tg Leu acc Thr 700 tct ttc gaa gag Ser Phe Giu Giu gct cgc aca gta Aia Arg Thr Val 720 gcc gat gag atc agc Aia Asp Giu Ile Ser 725 715 1373 WO 01/00843 WO 0100843PCTIBOO/00923 tac cca 2323 Tyr Pro gag att 2371 Giu Ile act gag 2419 Thr Giu aac gct 2467 Asn Ala 775 tac ctg 2515 Tyr Leu 790 ggt gac 2563 Gly Asp atc gag 2611 Ile Giu gta cag 2659 Val Gin tac gtc 2707 Tyr Val 855 tcc aag 2755 Ser Lys 870 gtg ggc 2803 Val Gly gag tac 2851 gtg ctg gtt cgc cct Val Leu Val Arg Pro 730 gtc tac gat gag gct Val Tyr Asp Giu Ala 745 ttg tct tct gac cac Leu Ser Ser Asp His 760 att gag atc gac gtc Ile Giu Ilie Asp Val 780 gca ggc gtc atg gag Aia Gly Vai Met Glu tcc tac gtc Ser Tyr Val 735 tcc ctc gag Ser Leu Glu ttg ggt ggc cgt ggc atg Leu Gly Gly Arg Gly Met 740 gat tac atc aac cgc gca Asp Tyr Ile Asn Arg Ala 755 gtt gac cgc ttc cta gac Val Asp Arg Phe Leu Asp 770 cca Pro 765 gac Asp cac His ctg Leu gca ctg tgc gac ggc gac gag gtc Ala Leu Cys Asp Giy Asp Giu Val 785 atc gag gaa gcc ggc att cac tcc Ile Giu Giu Ala Giy Ile His Ser tcc gca tgt Ser Ala Cys 810 aag gtc cgc Lys Vai Arg 825 ggc ctg atg Giy Leu Met 840 800 ctt cct cca atg act Leu Pro Pro Met Thr 815 805 ttg ggc gca cag gac Leu Gly Ala Gin Asp 820 gaa Glu gca Ala acc aag Thr Lys 830 aac gtc cag Asn Val Gin 845 tac Tyr gca Ala aag ctg gct ctg ggc atc ggt Lys Leu Ala Leu Gly Ile Gly 835 gca ctc aag gac gac atc ctc Ala Leu Lys Asp Asp Ile Leu 850 tcc cgc acc gtg ccg ttc gtc Ser Arg Thr Val Pro Phe Val gag gca aac cca Glu Ala Asn Pro 860 cgt Arg 865 gca acg ggc gtc Ala Thr Gly Val 875 gcc acc atc aag Ala Thr Ile Lys aac ctg gcc aag gca Asn Leu Ala Lys Ala 880 gat ctc caa gat gag Asp Leu Gin Asp Glu 895 ttg cca ctg gac gct Leu Pro Leu Asp Ala 910 gca tcc Ala Ser cgt atc gca Arg Ile Ala 885 890 gac ggc ggc ggc atg att cct acc Gly Met Ile Pro Thr 900 cca atc gct gtg aag Pro Ile Ala Val Lys 915 tc c Giu Tyr Asp Gly Gly Ser 905 1374 WO 01/00843 WO 0100843PCT/EBOO/00923 gaa gca 2899 Glu Ala ctg gac 2947 Leu Asp 935 ggc ttg 2995 Gly Leu 950 gcg ttt 3043 Ala Phe aac cgc 3091 Asn Arg atg ggc 3139 met Gly cgc aac 3187 Arg Asn 1015 gaa ggt 3235 Giu Gly 1030 gtt gac 3283 Val ASP gat ggc 3331 Asp Gly atc acc 3379 Ilie Thr ctg cgt 3427 Leu Arg 1095 gtg Val 920 acc Thr gcc Ala ttg ccg ttc aac cgc Leu Pro Phe Asn Arg 925 ctg ctt tcc cca gag Leu Leu Ser Pro Giu 940 aac aac ttc ggc gct Asn Asn Phe Gly Ala ttc cgt cgc cca gat gga aag acc Phe Arg Arg Pro Asp Gly Lys Thr 930 atg aag tcc act ggc gag gtc atg Met Lys Ser Thr Gly Glu Val Met gca tat gca Ala Tyr Ala 960 gct gaa gct ggc Ala Giu Ala Gly 965 955 ggt gca ttg cca Gly Ala Leu Pro 970 acc gaa ggc acc Thr Giu Gly Thr 975 gtc Val ttc gtg acc gtg gct Phe Val Thr Val Ala 980 gac aag Asp Lys 985 cgc acc ctg atc Arg Thr Leu Ile 9: tac aag atc ctc gcc acc g Tyr Lys Ile Leu Ala Thr G 1000 1005 ggc att gag tgt gaa gtt g' Gly Ile Giu Cys Glu Val V 1020 gta gag ggc aag tcc atc g' Val Giu Gly Lys Ser Ile V 1035 ctc atc ctc aac acc cca gi Leu Ile Leu Asn Thr Pro A 1050 tac gat atc cgc gca gca g4 Tyr Asp Ile Arg Ala Ala A tg cca atc cag cgc ctg gcg ttg eu Pro Ile Gin Arg Leu Ala Leu 90 995 aa ggc acc gca ggc atg ctg cgc iu Gly Thr Ala Gly Met Leu Arg 1010 tg ctc aag gct tccgac atc cgc al Leu Lys Ala Ser Asp le*Arg tg gat cgt al Asp Arg 10 ct ggt tct la Gly Ser 1055 c.a gtg acc la Val Thr 1.070 c.a gct gtc la Ala Val atc Ile )40 cgc Arg gaa ggc gaa Glu Gly Glu 1045 1065 act gtt cag Thr Val Gin ggt gtc acc g~ Gly Val Thr A: 1085 gct ggc gct cgc cac Ala Gly Ala Arg His 1060 gtg ggt gtt ccg ctg Val Gly Val Pro Leu 1075 cag ggc ata gag gcc Gin Gly Ile Glu Ala 1090 cag gaa ctc gac cac 1080 gag ggc gtt gtc agc gtc cgc gcg ctg Giu Gly Val Val Ser Val Arg Ala Leu Gin Glu Leu Asp His 1100 1105 1375 WO 01/00843 gca gtc aag gct taagccctat gacattcggc gag 3462 Ala Val Lys Ala 1110 <210> 1000 <211> 1113 <212> PRT <213> Corynebacterium giutamicum PCTA~BOO/00923 <400> 1000 Met Pro Lys Arg Ser Asp Ile Asn His Ile Cys Asn Vai Ile Gin Giu Arg 130 Gly Glu Thr Giu Leu 210 Met Val Val1 Arg Pro Giu Giu Thr Lys 115 Gly Giu Thr Met Arg 195 Ile Arg Asp Ile Vali Ala Pro Gin Ala 100 Tyr Glu Ser Val Gly 180 Ile Glu Asp Aia Thr Giy Leu Thr Ile Gly Leu Gly Asp Ala Ala 165 Gly Ala Giu Thr Leu 245 Leu Gin Lys Ile Glu His Asn Val Arg Arg 150 Glu Leu Gly Ser Ala 230 Gly Thr Ala Giu Met 55 Pro Pro Ala Giu Gin 135 Ser Leu Gly Gly Ile 215 Asp Val Asp Cys Giu 40 Thr Giu Ile Ala Leu 120 Lys Arg Gly Ser Gly 200 Leu Asn His Arg Giu 25 Gly Asp Tyr Asp Ile 105 Ile Phe Val1 Leu Gly 185 Leu Gly Val Thr Giu Val1 10 Phe Leu Pro Ile Ala 90 Gin Gly Lys Cys Pro 170 Leu Ala Trp Val G ly 250 Phe Leu Asp Arg Giu Asp 75 Val1 Leu Aia Asp His 155 Vai Ala Ala Lys Val 235 Asp Gin Val Val Met Lys Leu Asp Asp Ile 140 Asn Vai Tyr Ser Giu 220 Ile Ser Lys Ile Ser Thr Ala Ile Ala Arg Ile 125 Val Met Val Asn Pro 205 Phe Cys Val Met Gly Giy Leu Asp Phe Thr Leu 110 Asp Thr Giu Arg Thr 190 Giu Giu Ser Thr Arg Ser Thr Ile His Ala Leu Gly Ala Thr Giu Pro 175 Giu Ala Leu Ile Val1 255 Asp Gly Gin Asn Thr Lys Gly Ile Ile Ile Vai 160 Ser Asp Asn Giu Glu 240 Ala Gin Pro Ala Leu 1376 WO 01/00843 WO 0100843PCTIIBOO/00923 260 265 270 Gly Ile Ala Ile Ile Arg Giu Val Gly Val Asp Thr Gly Gly Cys Asn 275 280 285 Ile Gin Phe Ala Ile Asn Pro Val Asp Gly Arg Ile Ile Thr Ile Giu 290 295 300 Met Asn Pro Arg Val Ser Arg Ser Ser Ala Leu Ala Ser Lys Ala Thr 305 310 315 320 Gly Phe Pro Ile Ala Lys Met Ala Ala Lys Leu Ala Ile Gly Tyr Thr 325 330 335 Leu Asp Giu Ile Thr Asri Asp Ile Thr Gly Giu Thr Pro Ala Ala Phe 340 345 350 Giu Pro Thr Ile Asp Tyr Vai Vai Val Lys Ala Pro Arg Phe Ala Phe 355 360 365 Glu Lys Phe Val Gly Ala Asp Asp Thr Leu Thr Thr Thr Met Lys Ser 370 375 380 Val Gly Giu Val Met Ser Leu Gly Arg Asn Tyr Ile Ala Ala Leu Asn 385 390 395 400 Lys Ala Leu Arg Ser Leu Giu Thr Lys Gin Gin Gly Phe Trp Thr Lys 405 410 415 Pro Asp Glu Phe Phe Ala Gly Giu Arg Ala Thr Asp Lys Ala Ala Val 420 425 430 Leu Giu Asp Leu Lys Arg Pro Thr Giu Gly Arg Leu Tyr Asp Val Giu 435 440 445 Leu Ala Met Arg Leu Gly Ala Ser Val Giu Giu Leu Tyr Giu Ala Ser 450 455 460 Ser Ile Asp Pro Trp Phe Leu Ala Giu Leu Giu Ala Leu Val Gin Phe 465 470 475 480 Arg Gin Lys Leu Val Asp Ala Pro Phe Leu Asn Giu Asp Leu Leu Arg 485 490 495 Giu Ala Lys Phe Met Gly Leu Ser Asp Leu Gin Ile Ala Ala Leu Arg 500 505 510 Pro Giu Phe Ala Gly Giu Asp Gly Val Arg Thr Leu Arg Leu Ser Leu 515 520 525 Gly Ile Arg Pro Val Phd Lys Thr Val Asp Thr Cys Ala Ala Giu Phe 530 535 540 Giu Ala Lys Thr Pro Tyr His Tyr Ser Ala Tyr Giu Leu Asp Pro Ala 545 550 555 560 Ala Glu Ser Glu Val Ala Pro Gin Thr Giu Arg Giu Lys Val Leu Ile 565 570 575 Leu Gly Ser Gly Pro Asn Arg Ile Gly Gin Gly Ile Glu Phe Asp Tyr 580 585 590 1377 WO 01/00843 WO 0100843PCTIBOO/00923 Ser Cys Val His Ala Ala Leu Glu Leu Ser Arg Val Gly Tyr Giu Thr 595 600 605 Val Ala 625 Val1 Gin Ala Glu Ala 705 Ala Gly Tyr Asp Asp 785 Ala Leu Ala Lys Thr 865 Al a Gly Met 610 Asp Tyr Leu Gly Asp 690 Pro Asp Giy Ile Arg 770 Giy Gly Giy Leu Asp 850 Val Ser Met Val Arg His C ly Val1 675 Arg Ala Glu Arg Asn 755 Phe Asp Ile Ala Gly 835 Asp Pro Arg Ile Asn Leu Ala Gly 660 Pro Gly Phe Ile Gly 740 Arg Leu Giu His Gin 820 Ile Ile Phe Ile Pro 900 Pro 615 Glu Gin Pro Gly Gly 695 Ala Pro Ile Glu Ala 775 Leu Asp Glu Gin Val 855 Lys Gly Tyr Glu Pro Ser Leu Thr 680 Ala Thr Val Val1 Leu 760 Ile Ala Ser Lys Gly 840 Ile Ala Ala

ASP

Thr Leu Gly Gly 665 Ser Leu Ser Leu Tyr 745 Ser Giu Gly Ala Val 825 Leu Glu Thr Thr Gly 905 Val1 Thr Thr 650 Leu Pro Leu Phe Val 730 Asp Ser Ile Val Cys 810 Arg Met Ala Giy Ile 890 Giy Ser Phe 635 Val Ala Giu Asn Glu 715 Arg Glu Asp Asp Met 795 Ala Glu Asn Asn Val 875 Lys Ser Thr 620 Glu Ala Asp Al a Arg 700 Glu Pro Ala His Val 780 Giu Leu Ala Val Pro 860 Asn Asp Leu Asp

ASP

Gly Arg Ile 685 Giu Ala Ser Ser Pro 765 Asp His Pro Thr Gin 845 Arg Leu Leu Pro Tyr Val1 Val1 Leu 670 Asp Gin Arg Tyr Leu 750 Val Ala Ile Pro Lys 830 Tyr Ala Ala Gin Leu 910 Asp Met Ile 655 Lys Met Leu Thr Val 735 Giu Leu Leu Giu Met 815 Lys Ala Ser Lys Asp 895

ASP

Thr Giu 640 Val Lys Ala Pro Val1 720 Leu Asp Val Cys Giu 800 Thr Leu Leu Arg Ala 880 Giu Ala 1378 WO 01/00843 PCTIiBOO/00923 Pro Ile Ala Val Lys Giu Ala Val Leu Pro Phe Asn Arg Phe Arg Arg 915 920 925 Pro Asp Gly Lys Thr Leu Asp Thr Leu Leu Ser Pro Giu Met Lys Ser 930 935 940 Thr Giy Giu Val Met Gly Leu Ala Asn Asn Phe Gly Ala Ala Tyr Ala 945 950 955 960 Lys Ala Giu Ala Gly Ala Phe Gly Ala Leu Pro Thr Glu Giy Thr Val 965 970 975 Phe Val Thr Vai Ala Asn Arg Asp Lys Arg Thr Leu Ile Leu Pro Ile 980 985 990 Gin Arg Leu Ala Leu Met Gly Tyr Lys Ile Leu Ala Thr Giu Gly Thr 995 1000 1005 Ala Gly Met Leu Arg Arg Asn Gly Ile Giu Cys Giu Val Val Leu Lys 1010 1015 1020 Ala Ser Asp Ile Arg Glu Gly Val Giu Gly Lys Ser Ile Val Asp Arg 1025 1030 1035 1040 Ile Arg Giu Gly Glu Val Asp Leu Ile Leu Asn Thr Pro Ala Gly Ser 1045 1050 1055 Ala Gly Ala Arg His Asp Gly Tyr Asp Ile Arg Ala Ala Ala Val Thr 1060 1065 1070 Val Gly Val Pro Leu Ile Thr Thr Val Gin Gly Val Thr Ala Ala Val 1075 1080 1085 Gin Gly Ile Giu Ala Leu Arg Giu Gly Val Val Ser Val Arg Ala Leu 1090 1095 1100 Gin Giu Leu Asp His Ala Val Lys Ala 1105 1110 <210> 1001 <211> 3221 <212> DNA <213> Corynebacteriui giutarnicum <220> <221> CDS <222> (1)..(3198) <223> FRXA02234 <400> 1001 ggc tcc aac cca gca acg atc atg acc gac cca gaa atg gct gac cac 48 Gly Ser Asn Pro Ala Thr Ile met Thr Asp Pro Giu Met Ala Asp His 1 5 10 acc tac gtg gag cca atc gag ccg gaa tac atc gac aag att ttc gct 96 Thr Tyr Val Glu Pro Ile Giu Pro Glu Tyr Ilie Asp Lys Ile Phe Ala 25 aag gaa atc gag cag ggc cac cca atc gac gcc gtc ctg gca acc ctt 144 Lys Glu Ilie Giu Gin Gly His Pro Ile Asp Ala Val Leu Ala Thr Leu 1379 WO 01/00843 WO 0100843PCTJIBOO/00923 ggt ggc Gly Gly cag act gca ctt Gin Thr Ala Leu gca gct atc cag Ala Ala Ile Gin gat cgc ctc ggc Asp Arg Leu Giy ctg gaa aag tac Leu Giu Lys Tyr ggc Gly gtt gaa ctc atc Val Giu Leu Ile ggt Gly gca gac atc gat Ala Asp Ile Asp att gag cgc ggc Ile Giu Arg Giy gat cgc cag Asp Arg Gin aag ttc Lys Phe aag gat att gtc Lys Asp Ile Val acc acc Thr Thr atc ggt ggc Ile Giy Gly gtc cac gag Vai His Giu gaa Giu 100 tcc gcg cgt tcc Ser Aia Arg Ser gtc tgc cac aac Val Cys His Asn atg gaa gaa Met Giu Glu 110 gtg cgt cca Vai Arg Pro act gtc gca gaa Thr Val Ala Giu ggc ctt cca gta Giy Leu Pro Vai gtc Val 125 tcc ttc Ser Phe 130 act atg ggt ggc Thr Met Giy Gly ggc tcc ggt ctt *Giy Ser Giy Leu gca Ala 14 0 tac aac acc gaa Tyr Asn Thr Giu gac Asp 145 ctt gag cgc atc Leu Giu Arg Ile ggt ggc gga ctt Gly Giy Gly Leu gca tct cct gaa Ala Ser Pro Glu aac gtc ttg atc Asn Val Leu Ile gaa Giu 165 gaa tcc atc ctt Giu Ser Ile Leu tgg aag gaa ttc Trp, Lys Giu Phe gag ctc Giu Leu 175 gag ctc atg Giu Leu Met gaa aac gtc Giu Asn Val 195 cgc Arg 180 gat acc gca gac Asp Thr Ala Asp gtt gtg gtt atc Val Val Val Ile tgc tcc att Cys Ser Ilie 190 gtc acc gtg Val Thr Val gac gca ctg ggc Asp Ala Leu Giy cac acc ggc gac His Thr Gly Asp tct Ser 205 gca cct Ala Pro 210 gcc ctg acc ctg Ala Leu Thr Leu gac cgt gaa ttc Asp Arg Giu Phe aag atg cgc gat Lys Met Arg Asp cag Gin 225 ggt atc gcc atc Gly Ile Ala Ilie cgc gag gtc ggc Arg Giu Val Giy gac acc ggt gga Asp Thr Gly Giy aac atc cag ttc Asn Ile Gin Phe gcc Ala 245 atc aac cca gtt Ile Asn Pro Val ggc cgc atc atc Gly Arg Ile Ile acc att Thr Ile 255 gag atg aac Giu Met Asn acg ggc ttc Thr Gly Phe 275 cgt gtg tct cgt Arg Vai Ser Arg tcc gct ctg gca Ser Ala Leu Aia tcc aag gca Ser Lys Aia 270 atc gga tac Ile Gly Tyr cca att gcc aag atg Pro Ile Ala Lys Met 280 gct gcc aag ctg Ala Aia Lys Leu 1380 WO 01/00843 WO 0100843PCTIIBOO/00923 acc ctg Thr Leu 290 ttt gag Phe Giu 305 ttc gag 1008 Phe Glu tcc gtc 1056 Ser Val aac aag 1104 Asn Lys aag cct 1152 Lys Pro 370 gtt ctg 1200 Val Leu 385 gag ctg 1248 Glu Leu tct tct 1296 Ser Ser ttc cgc 1344 Phe Arg cgc gaa 1392 Arg Glu 450 cgc cca 1440 Arg Pro 465 cta ggc 1488 gat gag atc acc Asp Glu Ile Thr gac atc act ggt Asp Ile Thr Gly acc cca gct gcg Thr Pro Ala Ala ccc acc atc Pro Thr Ile aag ttt gtc Lys Phe Val 325 ggt gag gtc Gly Glu Vai 340 gtc gtg gtc Val Val Val cca cgc ttt Pro Arg Phe gct Ala 320 960 gct gat gac Ala Asp Asp tcc ctg ggc Ser Leu Gly act Thr 330 acc acc acc atg aag Thr Thr Thr Met Lys 335 atg Met 345 gca ctg cgt tcc ctg gaa Ala Leu Arg Ser Leu Glu 355 360 gat gag ttc ttc gca ggg Asp Glu Phe Phe Ala Gly acc Thr gag Glu cgc aac tac att gca gca ctg Arg Asn Tyr Ile Ala Ala Leu 350 aag cag cag ggt ttc tgg acc Lys Gin Gin Gly Phe Trp Thr 365 cgc gct acc gat aag gca gct Arg Ala Thr Asp Lys Ala Ala 380 gaa gat ctc aag Glu Asp Leu Lys 390 gca atg cgc ctt Ala Met Arg Leu 405 att gat cct tgg Ile Asp Pro Trp cca acc gaa ggc Pro Thr Giu Gly 395 cgc Arg ctc tac gac gtt Leu Tyr Asp Val 400 ggc gca agc gtg Gly Ala Ser Val 410 ttc ctc gcc gag Phe Leu Ala Glu gaa gaa ctc tac gaa gca Glu Glu Leu Tyr Glu Ala 415 ctt gaa gct ctc gtg cag Leu Glu Ala Leu Val Gin 430 420 cag aag Gin Lys 425 ctc gtt gac Leu Val Asp gca cca ttc cta aac gaa gat ctc ctg Ala Pro Phe Leu Asn Glu Asp Leu Leu 440 445 ctg tcc gac ctg cag atc gca gcc ctt Leu Ser Asp Leu Gin Ile Ala Ala Leu gca aag ttc Ala Lys Phe gag ttc gct Glu Phe Ala atg ggt met Gly 455 ggc Gly 470 gaa gac ggc gta cgc Glu Asp Gly Val Arg 475 ttc aag act gtg gat ttg cgt ctg Leu Arg Leu tcc Ser 480.

gag atc cgc cca gta acc tgt gca gca 1381 WO 01/00843 WO 0100843PCTA0iB00/00923 Leu Giy Ile Arg Pro Val Phe Lys Thr Val Asp Thr Cys Ala Ala Giu ttt gaa 1536 Phe Glu gca gct 1584 Ala Ala atc ttg 1632 Ile Leu 530 tac tcc 1680 Tyr Ser 545 act gtc 1728 Thr Val acc gct 1776 Thr Ala gag gtc 1824 Glu Val gtc cag 1872 Val Gin 610 aag gct 1920 Lys Ala 625 gct gag 1968 Aia Giu cct gct 2016 Pro Ala gta gcc 2064 Val Ala gct aag Ala Lys 500 gag tct Giu Ser ccg tac cac tac Pro Tyr His Tyr 505 tcc gca tac gag ctg gat cca Ser Ala Tyr Glu Leu Asp Pro 510 gag gtc gca Giu Val Ala ggt cca aac Gly Pro Asn cca Pro 520 cag Gin act gag cgt Thr Glu Arg tcc Ser 535 cgc atc ggc cag ggc Arg Ile Gly Gin Gly 540 ctt gag ctc tcc cgc Leu Giu Leu Ser Arg tgt gtt cac Cys Val His atg gtc aac Met Val Asn 565 gac cgc ctg Asp Arg Leu 580 gca Ala 550 gct Al~a tgc aac cca gag acc Cys Asn Pro Giu Thr 570 tac ttc gag cca ctg Tyr Phe Glu Pro Leu 585 gag gcg cag tcc ggc Giu Ala Gin Ser Gly acc ttc Thr Phe acc gtc Thr Val gaa aag gtc ctg Glu Lys Val Leu 525 atc gag ttc gac Ile Giu Phe Asp gtc ggc tac gaa Val Gly Tyr Giu 560 acc gac tac gac Thr Asp Tyr Asp 575 gaa gac gtc atg Giu Asp Val Met 590 gca ggt gtt atc Ala Gly Val Ile 605 gat cgt ttg aag Asp Arg Leu Lys gca atc gac atg Ala Ile Asp Met 640 cgc gag cag ctt Arg Glu Gin Leu 655 cac gct His Ala 600 ctt ggt ggc cag act Leu Gly Gly Gin Thr 615 ggc gtc cct gtc att Gly Val Pro Val Ile cct ctg ggc ttg gca Pro Leu Gly Leu Ala 620 ggt acc tcc cca gag Gly Thr Ser Pro Glu 635 ggt gca ctg ctg aac Gly Ala Leu Leu Asn gac cgt ggc Asp Arg Gly 645 cca gca ttc Pro Ala Phe 660 ttc Phe ggc Gly.

acc gca acc Thr Ala Thr 650 gat gag atc agc tac Asp Glu Ile Ser Tyr 665 cca gtg Pro Val tct ttc gaa gag gct cgc aca Ser Phe Giu Glu Ala Arg Thr 670 ctg gtt cgc cct tcc tac gtc Leu Val Arg Pro Ser Tyr Val 1382 WO 01/00843 WO 0100843PCT/iBOO/00923 ttg ggt 2112 Leu Gly 690 gat tac 2160 Asp Tyr 705 gtt gac 2208 Val Asp tgc gac 2256 Cys Asp gaa gcc 2304 Giu Ala act ttg 2352 Thr Leu 770 ctg gct 2400 Leu Ala 785 ctc aag 2448 Leu Lys cgc acc 2496 Arg Thr gca gca 2544 Ala Ala gag ggc 2592 Glu Gly 850 gct cca 2640 Ala Pro 865 cgt ggc atg gag Arg Gly Met Giu 695 gtc tac gat Val Tyr Asp atc aac cgc Ile Asn Arg cgc ttc cta Arg Phe Leu 725 ggc gac gag Gly Asp Giu gca Ala 710 act Thr gag ttg tct tct Glu Leu Ser Ser 715 685 gag gct tcc ctc gag Glu Ala Ser Leu Glu 700 gac cac cca gtg ctg Asp His Pro Val Leu 720 gac gtc gac gca ctg Asp Val Asp Ala Leu 735 atg gag cac atc gag met Glu His Ile Glu gac aac gct att Asp Asn Ala Ile gtc tac ctg gca Val Tyr Leu Ala 745 tcc ggt gac tcc Ser Cly Asp Ser gag Glu 730 ggC Gly atc Ile gtc Val 740 ggc att Gly Ile cac His 750 gca tgt gca ctt cct cca atg Ala Cys Ala Leu Pro Pro Met 765 gtc cgc gaa gca acc aag aag Val Arg Glu Ala Thr Lys Lys 755 760 ggc gca cag gac atc Gly Ala Gln Asp Ile 775 ctg ggc atc ggt gta Leu Gly Ile Gly Val 790 gag aag Glu Lys cag ggc ctg atg Gln Gly Leu Met 795 780 aac Asn gac gac atc Asp Asp Ile 805 gtg ccg ttc Val Pro Phe 820 tcc cgt atc Ser Arg Ile ctc tac gtc atc gag gca aac Leu Tyr Val Ile Glu Ala Asn 810 gtc tcc aag gca acg ggc gtc Val Ser Lys Ala Thr Gly Val gtc cag tac gca Val Gln Tyr Ala 800 cca cgt gca tcc Pro Arg Ala Ser 815 aac ctg gcc aag Asn Leu Ala Lys 830 825 gca gtg ggc gcc Ala Val Gly Ala 840 acc gag tac gac Thr Glu Tyr Asp acc atc aag gat ctc caa gat Thr Ile Lys Asp Leu Gln Asp 845 ggc ggc tcc ttg cca ctg gac Gly Gly Ser Leu Pro Leu Asp att cct Ile Pro 855 atc gct gtg aag gaa gca 860 gtg ttg ccg ttc Val Leu Pro Phe aac cgc Asn Arg Ile Ala Val Lys 870 Clu Ala ttc cgt Phe Arg 880 875 1383 WO 01/00843 WO 0100843PCT/EBOO/00923 cgc cca 2688 Arg Pro tcc act 2736 Ser Thr gca aag 2784 Ala Lys gtc ttc 2832 Val Phe 930 atc cag 2880 Ile Gin 945 acc gca 2928 Thr Ala aag gct 2976 Lys Ala cgt atc 3024 Arg Ile tct gct 3072 Ser Ala 1010 acc gtg 3120 Thr Val 1025 gtc cag 3168 Val Gin gat gga aag Asp Gly Lys 885 ggc gag gtc Gly Giu Val acc ctg gac acc ctg Thr Leu Asp Thr Leu 890 atg ggc ttg gcc aac Met Giy Leu Ala Asn 905 ctt tcc cca gag Leu Ser Pro- Glu atg aag Met Lys 895 aac ttc ggc gct gca tat Asn Phe Giy Ala Ala Tyr 910 900 gct gaa Ala Giu gct ggc gcg ttt Ala Giy Ala Phe 920 915 ggt gca ttg cca acc gaa ggc acc Gly Ala Leu Pro Thr Giu Gly Thr 925 gac aag cgc acc ctg atc ctg cca Asp Lys Arg Thr Leu Ile Leu Pro 940 gtg acc gtg gct aac Val Thr Val Ala Asn 935 cgc Arg cgc ctg gcg ttg Arg Leu Ala Leu 950 ggc atg ctg cgc Gly Met Leu Arg 965 tcc gac atc cgc Ser Asp Ile Arg atg Met ggc tac aag atc Gly Tyr Lys Ile 955 ctc Leu gcc acc gaa ggc Ala Thr Giu Gly 960 cgc aac ggc att Arg Asn Gly Ile 970 gaa ggt gta gag Giu Gly Val Glu gag tgt gaa gtt gtg ctc Giu Cys Giu Val Val Leu 975 ggc aag tcc atc gtg gat Gly Lys Ser Ile Val Asp 990 980 cgc Arg 995 gaa ggc gaa gtt gac Glu Gly Giu Val Asp 1000 985 ctc Leu ggc gct cgc cac gat ggc tac Gly Ala Arg His Asp Gly Tyr 10i5 ggt gtt ccg ctg atc acc act Gly Val Pro Leu Ile Thr Thr 1030 ggc ata gag gcc ctg cgt gag Gly Ile Giu Ala Leu Arg Giu 1045 atc ctc aac acc cca gct ggt Ile Leu Asn Thr Pro Ala Gly 1005 gat atc cgc gca gca gca gtg Asp Ile Arg Ala Ala Ala Val 1020 gtt cag ggt gtc acc gca gct Val Gin Gly Val Thr Ala Ala 1035 1040 ggc gtt gtc agc gtc cgc gcg Gly Val Val Ser Vai Arg Ala 1050 1055 gct taagccctat gacattcggc Ala ctg cag gaa 3218 ctc gac cac gca gtc aag Lys 106 Leu Gin Giu Leu Asp His Ala Val 1060 1384 WO 01/00843 WO 0100843PCT/IBOO/00923 gag 3221 <210> 1002 <211> 1066 <212> PRT <213> Corynebacterium giutamicum <400> 1002 Gly Ser Asn Pro Ala Thr Ile Met Thr 1 Thr Lys Gly Ile Ilie Ile Val1 Ser Asp 145 Asn Giu Giu Ala Gin 225 Asn Glu Val1 Ile Gin Giu Arg Giy Giu 115 Thr Giu Leu Met Val1 195 Ala Ilie Gin Asn Giu Glu Thr Lys Gly Glu 100 Thr Met Arg Ile Arg 180 Asp Leu Ala Phe Pro 260 5 Pro Gin Ala Tyr Giu Ser Vai Gly Ile Giu 165 Asp Ala Thr Ile Ala 245 Arg Giu 25 Ile Ala Leu Lys Arg 105 Gly Ser Gly Leu Asn 185 His Arg Val Val Ser 265 Asp 10 Tyr Asp Ile Ile Phe 90 Val Leu Gly Leu Gly 170 Val Thr Giu Gly Asp 250 Ser Pro Glu Met Ile Asp Ala Val Gin. Leu Gly Ala 75 Lys Asp Cys His Pro Val Leu Ala 140 Ala Ala 155 Trp Lys Val Val Gly Asp Phe Gin 220 Val Asp 235 Gly Arg Lys Leu Asp Asp Ile Asn Val 125 Tyr Ser Giu Ile Ser 205 Lys Thr Ile Ala Ile Aia Arg Ile Val Met 110 Val Asn Pro Phe Cys 190 Val1 Met Gly Ile Asp Hi Phe Al Thr Le Leu Gi Asp Al 8 Thr Th Giu Gl Arg Pr Thr Gi Giu Ai 16 Giu Le 175 Ser Ii Thr Va Arg As, Gly Cy 24 Thr Ill 255 Ala Leu Ala Ser Lys Ala 270 1385 WO 01/00843 Thr Giy Phe 275 Thr Leu Asp 290 Phe Giu Pro 305 Phe Giu Lys Ser Val Gly Asn Lys Ala 355 Lys Pro Asp 370 Vai Leu Giu 385 Giu Leu Ala Ser Ser Ile Phe Arg Gin 435 Arg Giu Ala 450 Arg Pro Giu 465 Leu Giy Ilie Phe Giu Ala Ala Aia Giu 515 Ile Leu Gly 530 Tyr Ser Cys 545 Thr Val Met Thr Aia Asp Pro Giu Thr Phe Giu 340 Leu Giu Asp Met Asp 420 Lys Lys Phe Arg Lys 500 Ser Ser Val1 Val1 Arg 580 Ile Ile Ile Val 325 Val Arg Phe Leu Arg 405 Pro Leu Phe Ala Pro 485 Thr Giu Gly His Asn 565 Leu Ala Thr Asp 3.10 Gly Met Ser Phe Lys 390 Leu Trp Val Met Gly 470 Vai Pro Val Pro Aia 550 Cys Tyr Ala Ile Val1 Asp Gly 345 Thr Glu Thr Ser Ala 425 Pro Ser Giy Thr Tyr 505 Gin Ile Giu Giu Pro 585 Ala Thr Val1 Thr 330 Arg Lys Arg Giu Val 410 Giu Phe Asp Val1 Val 490 Ser Thr Gly Leu Thr 570 Leu Lys Gly Lys 315 Leu Asn Gin Ala Gly 395 Giu Leu Leu Leu Arg 475 Asp Ala Giu Gin Ser 555 Val1 Thr PCTIIBOO/00923 Gly Tyr Ala Ala Phe Ala 320 Met Lys 335 Ala Leu Trp Thr Ala Ala Asp Val 400 Giu Ala 415 Val Gin Leu Leu Ala Leu Leu Ser 480 Ala Giu 495 Asp Pro Val Leu Phe Asp Tyr Giu 560 Tyr Asp 575 Val met Giu Val Tyr His Ala Giu Ala Gin Ser Gly Thr Val Ala Gly Val Ile 1386 WO 01/00843 WO 0100843PCTIiBOO/00923 Val1 Lys 625 Ala Pro Val1 Leu Asp 705 Val1 Cys Giu Thr Leu 785 Leu Arg Ala Giu Ala 865 Arg Ser Ala Gln 610 Ala Glu Ala Ala G ly 690 Tyr Asp Asp Ala Leu 770 Ala Lys Thr Al a Gly 850 Pro Pro Thr Lys 595 Leu Gly Asp Pro Asp 675 Gly Ile Arg Gly Gly 755 Gly Leu Asp Val Ser 835 Met Ile Asp Gly Ala 915 Gly Gly Gin Thr Pro Leu Gly Leu Ala Asp Arg Leu Lys Val1 Arg Ala 660 Glu Arg Asn Phe Asp 740 Ile Ala Gly Asp Pro 820 Arg Ile Ala Gly Glu 900 Glu Pro Gly 645 Phe Ile Gly Arg Leu 725 Glu His Gin I le Ile 805 Phe Ile Pro Val Lys 885 Val Ala Val 630 Glu Gly Ser Met Ala 710 Asp Val Ser Asp Gly 790 Leu Val Ala Thr Lys 870 Thr Met Gly 615 Ile Phe Thr Tyr Glu 695 Thr Asn Tyr Gly Ile 775 Val Tyr Ser Val Clu 855 Giu Leu Gly Ala Gly Gly Ala Pro 680 Ile Giu Ala Leu Asp 760 Glu Gin Val Lys Gly 840 Tyr Ala Asp Leu Phe 920 Pro 635 Leu Phe Val Asp Ser 715 Ile Val Cys Arg Met 795 Ala Gly Ile Gly Pro 875 Leu Asn 620 Glu Ala Asn Arg Giu Glu Arg Pro 685 Giu Ala 700 Asp His Asp Val Met Giu Ala Leu 765 Giu Ala 780 Asn Val Asn Pro Val Asn Lys Asp 845 Ser Leu 860 Phe Asn Ser Pro Phe Gly Ile Giu Ala 670 Ser Ser Pro Asp His 750 Pro Thr Gin Arg Leu 830 Leu Pro Arg Giu Ala 910 Asp Gin 655 Arg Tyr Leu Val1 Ala 735 Ile Pro Lys Tyr Ala 815 Ala Gin Leu Phe Met 895 Ala Ala Leu Pro Thr Glu Gly Thr 925 1387 WO 01/00843 Val Phe Val 930 Ile Gin Arg 945 Thr Ala Giy Lys Ala Ser Arg Ilie Arg 995 Ser Ala Gly 1010 Thr Val Gly 1025 Val Gin Gly Leu Gin Giu Thr Val Ala Asri Arg Asp Lys Arg Thr Leu Ile 935 940 Leu Ala Leu Met Gly Tyr Lys Ile Leu Aia Thr 950 955 Met Leu Arg Arg Asn Gly Ile Giu Cys Giu Val 965 970 Asp Ile Arg Giu Gly Val Giu Gly Lys Ser Ile 980 985 990 Giu Gly Giu Val Asp Leu Ile Leu Asn Thr Pro 1000 1005 Ala Arg His Asp Giy Tyr Asp Ile Arg Ala Ala 1015 1020 Val Pro Leu Ile Thr Thr Vai Gin Gly Val Thr 1030 1035 Ile Giu Ala Leu Arg Giu Giy Vai Val Ser Val 1045 1050 Leu Asp His Ala Val Lys Ala 1060 1065 PCTIBOO/00923 Leu Pro Giu Gly 960 Vai Leu 975 Val Asp Aia Gly Ala Vai Ala Ala 1040 Arg Ala 1055 <210> 1003 <211> 424 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS <222> (101)..(424) <223> RXN00450 <400> 1003 tttgcgatga catggatttg gatccttccg aacaattgct gttttgataa tgatctggct cgggtggtgg gattcgacta gtg Val gat Asp att Ilie gaa Giu gac Asp cc t Pro gag Giu acg Thr gag c Glu ggg Gly ctg q Le u cac q His .1 1388 cgc Arg gtt Val acc Thr gaa Glu gcgcatcgcg gaagaactcg gtg ggc gtt tta cct Val Gly Val Leu Pro 1 atg cgc cat gct ttg Met Arg His Ala Leu ccc gtt ggc gcc gtC Pro Vai Gly Ala Val gca acg aac cgt cga Ala Thr Asn Arg Arg att att gct tta cga Ile Ile Ala Leu Arg WO 01/00843 WO 0100843PCTflIBOO/00923 cga gcc gcc cgc cgt ttt tcc gac ggc tgg Cgg ctg agt gac tgc acc 355 Arg Ala Ala Arg Arg Phe Ser Asp Gly Trp Arg Leu Ser Asp Cys Thr 75 80 gcg gtg gtc acc ttg gag ccc tgc agt atg tgc gcc ggc gcc ttg gtg 403 Ala Val Val Thr Leu Glu Pro Cys Ser Met Cys Ala Gly Ala Leu Val 95 100 ggt gct cga atc gga cgc atc 424 Gly Ala Arg Ile Gly Arg Ile 105 <210> 1004 <211> 108 <212> PRT <213> Corynebacterium glutamicun <400> 1004 Val Gly Val Leu Pro Val Gln Ala Arg Ile Lys Asp Asp Glu Arg Arg 1 5 10 Met Arg His Ala Leu Asp Ile Ala Arg Gln Thr Pro Glu Gly Asp Val 25 Pro Val Gly Ala Val Ile Tyr Ala Pro Thr Gly Glu Ile Leu Ala Thr 40 Ala Thr Asn Arg Arg Glu Ala Asp Arg Asp Pro Thr Ala His Ala Glu 55 Ile Ile Ala Leu Arg Arg Ala Ala Arg Arg Phe Ser Asp Gly Trp Arg 70 75 Leu Ser Asp Cys Thr Ala Val Val Thr Leu Glu Pro Cys Ser met Cys 90 Ala Gly Ala Leu Val Gly Ala Arg Ile Gly Arg Ile 100 105 <210> 1005 <211> 418 <212> DNA <213> Corynebacteriun glutamicum <22 0> <221> CDS <222> (101)..(418) <223> FRXA00450 <400> 1005 tttgcgatga catggatttg gatccttccg aacaattgct gcgcatcgcg gaagaactcg gttttgataa* tgatctggct cgggtggtgg gattcgacta gtg ggc gtt tta cct 115 Val Gly Val Leu Pro 1 gtg cag gcg cgc atc aaa gac gat gag cgc cgc atg cgc cat gct ttg 163 Val Gin Ala Arg Ile Lys Asp Asp Giu Arg Arg Met Arg His Ala Leu 1389 WO 01/00843 WO 0100843PCTIJIBOO/00923 15 gat att gct cgc caa acc cct gag ggg gac gtt ccc gtt ggc gcc gtc 211 Asp Ilie Ala Arg Gin Thr Pro Giu Gly Asp Val. Pro Val Gly Ala Val 30 att tac gcg ccg acc ggg gag atc ctg gcg acc gca acg aac cgt cga 259 Ilie Tyr Ala Pro Thr Gly Glu Ile Leu Ala Thr Ala Thr Asn Arg Arg 45 gaa gca gac cgc gat ccc acg gcc cac gcc gaa att att gct tta cga 307 Glu Ala Asp Arg Asp Pro Thr Ala His Ala Gu Ile Ile Ala Leu Arg 60 cga gcc gcc cgc cgt ttt tcc gac ggc tgg cgg ctg agt gac tgc acc 355 Arg Ala Ala Arg Arg Phe Ser Asp Gly Trp Arg Leu Ser Asp Cys Thr 75 80 gcg gtg gtc acc ttg gag ccc tgc agt atg tgc gcc ggc gcc ttg gtg 403 Ala Val Val Thr Leu Giu Pro Cys Ser Met CyS Ala Gly Ala Leu Val 95 100 ggt gct cga atc gga 418 Gly Ala Arg Ile Gly 105 <210> 1006 <211> 106 <212> PRT <213> Corynebacteriun glutaxnicum <400> 1006 Val Gly Val Leu Pro Val Gin Ala Arg Ile Lys Asp Asp Glu Arg Arg 1 5 10 Met Arg His Ala Leu Asp Ile Ala Arg Gin Thr Pro Glu Gly Asp Val 25 Pro Val Gly Ala Val Ile Tyr Ala Pro Thr Gly Giu Ile Leu Ala Thr 40 Ala Thr Asn Arg Arg Glu Ala Asp Arg Asp Pro Thr Ala His Ala Glu 55 Ile Ile Ala Leu Arg Arg Ala Ala Arg Arg Phe Ser Asp Gly Trp Arg 70 75 Leu Ser Asp Cys Thr Ala Val Val Thr Leu Glu Pro Cys Ser Met Cys 90 Ala Gly Ala Leu Val Gly Ala Arg Ile Gly 100 105 <210> 1007 <211> 1368 <212> DNA <213> Corynebacteriun glutamicun <22 0> 1390 WO 01/00843 WO 0100843PCT/BOOOO923 <221> CDs <222> (101)..(1345) <223> RXN02272 <400> 1007 tatttaactc cacactaata cctgtcaagc aatagaatag agtttgaaaa tatgccttga catgtagaaa tggagttctt atagcatctg gatcctccag gtg cgc att aca aac Val Arg Ile Thr Asn gcc cag gtt aag aac tac gca gag tta Ala Gin Val Lys Asn Tyr Ala Giu Leu gaa aaa att tcc tcg att acc ccc tct Giu Lys Ilie Ser Ser Ilie Thr Pro Ser 30 gat atc acc ata Asp Ile Thr Ile gag ggt Giu Gly tca att cga tca gaa gaa gat Ser Ile Arg Ser Giu Giu Asp cac cgc gcg His Arg Ala gac gat tac gat Asp Asp Tyr Asp gca gga aga ctg Ala Gly Arg Leu gca ccc cag Ala Pro Gin ttc gcc Phe Ala gaa gca cac atc Giu Ala His Ile ctt gac tac gca aac acc gct gga atc Leu Asp Tyr Ala Asn Thr Ala Gly Ilie cgc gaa aac tct Arg Giu Asn Ser ggc aca ctt ttt Gly Thr Leu Phe gaa Giu gcc atc gaa atc Ala Ile Giu Ile gcc gac cgc aag Ala Asp Arg Lys caa ggc ttc cac Gin Gly Phe His aaa gaa gac att Lys Giu Asp Ile aaa gcg Lys Ala 100 aag gcc ctc Lys Ala Leu atc cgc act Ile Arg Thr 120 cag Gin 105 gca gcc cgt cgg Ala Ala Arg Arg gca gaa cac ggc gtt ggt ttc Ala Giu His Gly Val Gly Phe 115 cac gta gat gtc His Val Asp Val gat ccc acg ttt Asp Pro Thr Phe gct Ala 130 gga ttc gaa Gly Phe Giu gca att Ala Ile 135 gcg gag ctg cgc Ala Giu Leu Arg gaa gtc cgc gag Giu Val Arg Giu tgg Trp 145 tgc gat atc cag Cys Asp Ile Gin gtc gcc ttc ccg Val Ala Phe Pro aat ggc att tac Asn Gly Ile Tyr tac gaa ggt ggc Tyr Giu Gly Gly aag cta atc tca Lys Leu Ile Ser gat Asp 170 gca atg tct gca Ala Met Ser Ala gca gat gtc gtt Ala Asp Val Val ggt ggc Gly Gly 180 atc cca cac Ile Pro His ctt Leu 185 gaa ccc acc cga Giu Pro Thr Arg gac Asp 190, gat ggc gtc gag Asp Gly Val Giu tcg gtg aaa Ser Val Lys 195 tgg ctg ttc gac ctt gca gag aag cac tca gcc ccc atc gat atc cac Trp Leu Phe Asp Leu Ala Giu Lys His Ser Ala Pro Ile Asp Ile His 1391 WO 01/00843 WO 0100843PCT/EBOO/00923 act gat Thr Asp 215 gca gaa Ala Giu 230 tct gtg Ser Val ccc aag Pro Lys aac ctc Asn Leu gtt gca 1027 Val Ala 295 tgc cag 1075 Cys Gin 310 cta ctc 1123 Leu Leu gcc agc 1171 Ala Ser.

gga aac 1219 Gly Asn aac ctg 1267 Asn Leu 375 aaa gct 1315 Lys Ala 390 agg gag 1365 Arg Glu 200 gaa Giu gcc Ala gcg Ala ctc Leu cat His 280 ccg Pro gac Asp cgc Arg cac His cta Leu 360 ctg Leu tcc Ser ccc Pro att Ile gca Ala atg Met gca Ala 265 ctg Leu gta Val tca Ser att Ile t tg Leu 345 ggc Gly gtt Val gta Val gaa Glu gac Asp aaa Lys gcc Al a 250 gca Ala caa Gin aag Lys c tc Leu ctc Leu 330 aag Lys ctg Leu ctt Leu ctt Leu cag Gin 410 gat Asp cgt Arg 235 tat Tyr tca Ser gga Gly c aa Gin aat Asn 315 gat Asp aat Asn gac Asp gat Asp t tg Leu 395 gtg Val1 cca Pro 220 gac Asp tac Tyr aag Lys ctt Leu ctt Leu 300 gac Asp tct Ser gca Ala aat Asn gcg Ala 380 agc Ser gac Asp 205 cat His atg Met tca Ser gtt Val ggt Gly 285 acc Thr ccc Pro gga Gly cta Leu tac Tyr 365 agc Ser atc Ile tgg Trp tcc Ser ggc Gly cct Pro cgt Arg 270 t tc Phe gaa Giu ttc Phe tta Leu tcg Ser 350 gac Asp agc Ser cac His aac Asn cga ttt Arg Phe gca caa Ala Gin 240 ggc tac Gly Tyr 255 ttt gca Phe Ala caa gga Gin Gly tgg gga Trp Gly tac ccc Tyr Pro 320 cac gtg His Val 335 ttc atc Phe Ile att gca Ile Ala gag aag Giu Lys cgc ggc Arg Gly 400 atc taac Ile gtc Val 225 acc Thr atg Met gta Val ccc Pro att Ile 305 atg Met tcc Ser acc Thr gaa Giu gaa Giu 385 aaa 210 gaa Giu gtg Val gcg Ala tgc Cys gtC Val 290 cca Pro ggc Gly.

cac His acc Thr aac Asn 370 gct Ala aag gtc ctc Val Leu gtg tct Val Ser cga ctt Arg Leu 260 ccc aat Pro Asn 275 ccc cga Pro Arg gta agt Val Ser gat gga Asp Gly atg ctc Met Leu 340 aat cca Asn Pro 355 tcc ccg Ser Pro gta cag Val Gin gtg ctc gcc Ala cat His 245 tta Leu gaa Giu ggt Gly tt t Phe gat Asp 325 aca Thr gcc Ala gcg Ala aga Arg tcc 787 835 883 931 979 Lys Lys Val Leu Ser 405 :agccca gttgggcctc 1392 WO 01/00843 PCT/IB00/00923 ctt 1368 <210> 1008 <211> 415 <212> PRT <213> Corynebacterium glutamicum <400> 1008 Val Arg Ile Thr Asn Ala Gin Val Lys Asn Tyr Ala Glu Leu Val Asp 1 5 10 Ile Thr Ile Glu Gly Glu Lys Ile Ser Ser Ile Thr Pro Ser Ser Ile 25 Arg Ser Glu Glu Asp His Arg Ala Asp Asp Tyr Asp Ala Ala Gly Arg 40 Leu Val Ala Pro Gin Phe Ala Glu Ala His Ile His Leu Asp Tyr Ala 55 Asn Thr Ala Gly Ile Pro Arg Glu Asn Ser Ser Gly Thr Leu Phe Glu 70 75 Ala Ile Glu Ile Trp Ala Asp Arg Lys Thr Gin Gly Phe His Ile Lys 90 Glu Asp Ile Lys Ala Lys Ala Leu Gin Ala Ala Arg Arg Ala Ala Glu 100 105 110 His Gly Val Gly Phe Ile Arg Thr His Val Asp Val Thr Asp Pro Thr 115 120 125 Phe Ala Gly Phe Glu Ala Ile Ala Glu Leu Arg Asp Glu Val Arg Glu 130 135 140 Trp Cys Asp Ile Gin Ile Val Ala Phe Pro Gin Asn Gly Ile Tyr Ala 145 150 155 160 Tyr Glu Gly Gly Gin Lys Leu Ile Ser Asp Ala Met Ser Ala Gly Ala 165 170 175 Asp Val Val Gly Gly Ile Pro His Leu Glu Pro Thr Arg Asp Asp Gly 180 185 190 Val Glu Ser Val Lys Trp Leu Phe Asp Leu Ala Glu Lys His Ser Ala 195 200 205 Pro Ile Asp Ile His Thr Asp Glu Ile Asp Asp Pro His Ser Arg Phe 210 215 220 Val Glu Val Leu Ala Ala Glu Ala Ala Lys Arg Asp Met Gly Ala Gin 225 230 235 240 Thr Val Val Ser His Ser Val Ala Met Ala Tyr Tyr Ser Pro Gly Tyr 245 250 255 Met Ala Arg Leu Leu Pro Lys Leu Ala Ala Ser Lys Val Arg Phe Ala 260 265 270 1393 WO 01/00843 WO 0100843PCTlLBOOIOO923 Val1 Pro Ile 305 Met Ser Thr Giu Giu 385 Lys Cys Val 290 Pro Gly His Thr Asn 370 Ala Lys Leu Val Ser Ile Leu 345 Gly Val1 Val Giu Gin Lys Leu Leu 330 Lys Leu Leu Leu Gin 410 Gly Gin Asn 315 Asp Asn Asp Asp Leu 395 Val1 Phe Giu Phe Leu Ser 350 Asp Ser His Asn Gin Trp Tyr His 335 Phe Ile Giu Arg Ile 415 Giy Giy Pro 320 Vai Ile Aia Lys Giy 400 <210> 1009 <21i> 1368 <212> DNA <213> Corynebacteriun giutanicuxn <220> <22i> CDS <222> (10i)..(1345) <223> FRXA02272 <400> 1009 tatttaactc cacactaata cctgtcaagc aatagaatag agtttgaaaa tatgccttga catgtagaaa tggagttctt gcc cag gtt aag Aia Gin Val Lys gaa Giu cac His ttc Phe aaa Lys cgc Arg gcc Aia gt t Vai tca Ser gga Giy tac Tyr gat Asp att Ile aga Arg gca Aila atagcatctg gatcctccag gtg cgc att aca aac Vai Arg Ile Thr Asn 1 atc acc ata gag ggt Ilie Thr Ile Giu Gly cga tca gaa-gaa gat Arg Ser Giu Giu Asp ctg gtc gca ccc cag Leu Vai Ala Pro Gin aac acc gct gga atc Asn Thr Aia Gly Ile 1394 WO 01/00843 WO 0100843PCTIiBOOIOO923 cct Pro cgc gaa aac tct Arg Glu Asn Ser ggc aca ctt ttt Gly Thr Leu Phe gcc atc gaa atc Ala Ile Giu Ile gcc gac cgc aag acc caa ggc ttc cac Ala Asp Arg Lys Thr Gin Gly Phe His aaa gaa gac att Lys Glu Asp Ile aaa gcg Lys Ala 100 aag gcc ctc Lys Ala Leu atc cgc act Ile Arg Thr 120 gca gcc cgt cgg gca gca gaa cac ggc gtt ggt ttc Ala Ala Arg Arg Ala Ala Giu His Gly Val Giy Phe cac gta gat gtc His Val Asp Vai gat ccc acg.ttt Asp Pro Thr Phe gct Ala 130 gga ttc gaa Gly Phe Glu gca att Ala Ilie 135 gcg gag ctg cgc Ala Glu Leu Arg gaa gtc cgc gag Giu Val Arg Giu tgg Trp 145 tgc gat atc cag Cys Asp Ile Gin att Ile 150 gtc gcc ttc ccg Val Ala Phe Pro aat ggc att tac Asn Gly Ile Tyr gcc tac gaa ggt ggc cag Ala Tyr Giu Gly Gly Gin 160 165 aag cta atc tca Lys Leu Ile Ser gat Asp 170 gca atg tct gca Ala Met Ser Ala gca gat gtc gtt Ala Asp Val Val ggt ggc Gly Gly 180 atc cca cac Ilie Pro His tgg ctg ttc Trp Leu Phe 200 ctt Leu 185 gaa ccc acc cga Glu Pro Thr Arg gat ggc gtc gag Asp Gly Val Giu tcg gtg aaa Ser Val Lys 195 gat atc cac Asp Ile His gac ctt gca gag Asp Leu Ala Glu cac tca gcc ccc His Ser Ala Pro atc Ile 210 act gat Thr Asp 215 gaa att gac gat Giu Ile Asp Asp cat tcc cga ttt His Ser Arg Phe gaa gtc ctc gcc Glu Val Leu Ala gaa gcc gca aaa Giu Ala Ala Lys gac atg ggc gca Asp Met Gly Ala acc gtg gtg tct Thr Val Val Ser tct gtg gcg atg Ser Val Ala Met gcc Ala 250 tat tac tca cct Tyr Tyr Ser Pro tac atg gcg cga Tyr Met Ala Arg ctt tta Leu Leu 260 ccc aag ctc Pro Lys Leu aac ctc cat Asn Leu His 280 gca tca aag gtt Ala Ser Lys Val ttt gca gta tgc Phe Ala Val Cys ccc aat gaa Pro Asn Glu 275 ccc cga ggt Pro Arg Gly ctg caa gga ctt Leu Gin Gly Leu ttc caa gga ccc Phe Gin Gly Pro gtc Val 290 gtt gca ccg gta aag caa ctt acc gaa tgg gga att cca gta agt ttt 1027 Val Ala 295 Pro Val Lys Gin Thr Giu Trp Gly Pro Val Ser Phe 1395 WO 01/00843 WO 0100843PCTA~BOO/00923 tgc cag 1075 Cys Gin 310 cta ctc 1123 Leu Leu gcc agc 1171 Ala Ser gga aac 1219 Gly Asn aac ctg 1267 Asn Leu 375 aaa gct 1315 Lys Ala 390 agg gag 1365 gac tca ctc aat Asp Ser Leu Asn 315 cgc att ctc gat Arg Ile Leu Asp gac ccc ttc tac ccc Asp Pro Phe Tyr Pro 320 tct gga tta cac gtg Ser Gly Leu His Val 335 atg ggc gat gga gat Met Gly Asp Gly Asp 325 tcc cac atg ctc aca Ser His Met Leu Thr 340 acc acc aat cca gcc Thr Thr Asn Pro Ala cac ttg His Leu 345 cta ggc Leu Gly aag aat gca cta tcg Lys Asn Ala Leu Ser 350 ctg gac aat tac gac Leu Asp Asn Tyr Asp 365 ttc atc Phe Ile 355 att gca gaa aac tcc ccg gcg Ile Ala Glu Asn Ser Pro Ala 370 gtt ctt gat Val Leu Asp gta ctt ttg Val Leu Leu gcg Ala 380 agc Ser agc agc gag aag gaa gct gta cag aga Ser Ser Glu Lys Giu Ala Val Gin Arg 385 atc cac cgc ggc aaa aag gtg ctc tcc Ile His Arg Gly Lys Lys Val Leu Ser tcc Ser 395 ccc gaa cag gtg 400 gac tgg aac atc taa 405 cagccca gttgggcctc Arg Glu Pro Glu Gin Val Asp Trp Asn Ile ctt 1368 <210> 1010 <211> 415 <212> PRT <213> Corynebacteriun glutamicun <400> 1010 Val Arg Ile Thr Asn Ala Gin Val Lys Asn Tyr 1 5 10 Ilie Thr Ilie Giu Gly Glu Lys Ile Ser Ser Ilie 25 Arg Ser Glu Glu Asp His Arg Ala Asp Asp Tyr Leu Val Ala Pro Gin Phe Ala Glu Ala His Ile 55 Asn Thr Ala Gly Ile Pro Arg Glu Asn Ser Ser 70 Ala Giu Leu Val Asp Thr Pro Asp His Gly Ser Ser Ile Ala Gly Arg Asp Tyr Ala Thr Leu Phe 1396 WO 01/00843 Ala Ilie Giu Giu Asp Ile His Gly Val 115 Phe Ala Gly 130 Trp Cys Asp 145 Tyr Glu Gly Asp Val Val Val Giu Ser 195 Pro Ile Asp 210 Val .Giu Val 225 Thr Val Val Met Ala Arg Val Cys Pro 275 Pro Val Pro 290 Ile Pro Val 305 Met Gly Asp Ser His Met Thr Thr Asn 355 Glu Asn Ser 370 Glu Ala Val 385 Arg Leu Thr 120 Ala Ala Ile His Phe 200 Glu Ala Al a Leu His 280 Pro Asp Arg His Leu 360 Leu Ser Lys Gin 105 His Giu Phe Ser Leu 185 Asp Ile Ala Met Ala 265 Leu Val Ser Ile Leu 345 Gly Val Val Thr Ala Val Leu Pro Asp 170 Giu Leu Asp Lys Ala 250 Ala Gin Lys Leu Leu 330 Lys Leu Leu Leu Gly Arg Val Asp 140 Asn Met Thr Giu Pro 220 Asp Tyr Lys Leu Leu 300 Asp Ser Ala Asn Ala 380 Ser Phe Arg Thr 125 Glu Gly Ser Arg Lys 205 His Met Ser Val Gly 285 Thr Pro Gly Leu Tyr 365 Ser Ile PCT/iBOO/00923 Ile Lys Ala Giu Pro Thr Arg Glu Tyr Ala 160 Gly Ala 175 Asp Gly Ser Ala Arg Phe Ala Gin 240 Gly Tyr 255 Phe Ala Gin Gly Trp Gly Tyr Pro 320 His Val 335 Phe Ile Ile Ala Giu Lys Arg Gly 400 Lys Lys Val Leu Ser Arg Glu Pro Giu Gin Val Asp Trp, Asn Ile 1397 WO 01/00843 WO 0100843PCT/IBOO/00923 <210> <211> <212> <213> <220> <221> <222> <223> 405 1011 580

DNA

Corynebacteriun glut aiicum

CDS

(101)..(580) RXN0 3004 <400> 1011 gctagcacga taaaaatcag cgccagcacc acgcgcccca ggaacctcat cgcagcaacc cgtggcccaa ccctgaacgc tgaacgctac actggttgac gtg ctt ctt tca Val Leu Leu Ser 1 cgt gac art cgt Arg Asp Ile Arg rca att gac gca Ser Ilie Asp Ala ggc Gly gac ttg gga at Asp Leu Gly Ile gaa cct Giu Pro ttc gac gct Phe Asp Ala ctg att cag ccg Leu Ile Gin Pro agt gtc gat gtc Ser Val Asp Val cgc atg gac Arg Met Asp att gac cct Ile Asp Pro cgc tac ttc cgg gtt ttc aat Arg Tyr Phe Arg Val Phe Asn tct aag tac Ser Lys Tyr acc cac Thr His aag ttg Lys Leu aat cag gat gag Asn Gin Asp Giu acc agc ctt gtt Thr Ser Leu Val gag Giu gtt gag gac ggc Val Giu Asp Gly ggc ttt gtg ctg Gly Phe Val Leu ggt gag ttt Gly Glu Phe ctg gcg tcc acg Leu Ala Ser Thr gaa aag ttc act Giu Lys Phe Thr cct gcg cat ctg Pro Ala His Leu ggt cgt ttg gag Gly Arg Leu Giu ggt aag Gly Lys 100 tcg tct ctt Ser Ser Leu att gat cct Ile Asp Pro 120 ggt Gly 105 cgt ctt ggc ttg Arg Leu Gly Leu acg cac tcr act gct ggt ttc Thr His Ser Thr Ala Gly Phe 115 ggt ttt agt ggt Gly Phe Ser Gly atc acg ttg gag Ile Thr Leu Giu ttg Leu 130 tcc aat gtg Ser Asn Val gct aat Ala Asn 135 ctg ccg arc acg Leu Pro Ile Thr tgg ccg ggt atg Trp Pro Gly Met gtg ggg cag ctg Val Giy Gin Leu ttg ttc cag atg Leu Phe Gin Met tcc cct gcg gag Ser Pro Ala Glu 1398 WO 01/00843 WO 0100843PCT/IBOO/00923 <210> 1012 <211> 160 <212> PRT <213> Corynebacterium giutarnicum <400> 1012 Val Leu Leu Ser 1 Leu Asp Thr Giu Leu Arg Ser Giu Lys 145 Gly Val His Val1 Ala Leu Thr Leu 130 Val Ile Arg Ile Giu Ser Giu Ala 115 Ser Giy Glu Met Asp Asp Thr Giy 100 Gly Asn Gin Asp 5 Pro Asp Pro Giy Leu Lys Phe Val Leu Arg Asp Ile Arg Phe Arg Lys Giu 70 Giu Ser Ile Ala Aia 150 Asp Tyr Leu 55 Giy Lys Ser Asp Asn 135 Leu Aila Phe Asn Phe Phe Leu Pro 120 Leu Phe Lys Leu Val Asp Leu Leu Arg Phe Ilie Met Ser Ile Phe Giu His Pro Leu Ser Thr Ser 155 Ile Gin Asn Leu Pro Aila Giy Giy Leu 140 Ser <210> 1013 <211> 225 <212> DNA <2i3> Corynebacteriun giutanicun <220> <221> CDS <222> (101)..(202) <223> RXN03137 <400> 1013 cggcgagttcatttggactg gcggcgactg ccacatttat gacaaccaca aggaacaggt cgcggagcag ctgagccgat aagctcgccc ctaccccacc ttg gag ctc aac aag 115 Leu Giu Leu Asn Lys 1 gca gcg tac atg ttt gag tac agc ttc gat gac atc acc gtg tcc ggc 163 Aia Ala Tyr Met Phe Giu Tyr Ser Phe Asp Asp Ile Thr Vai Ser Giy 15 1399 WO 01/00843 WO 0100843PCTA~BOO/00923 tac gat cca cac cca ttg atc cgc ggc aag gtc gcc gta tgatcggtgc Tyr Asp Pro Hius Pro Leu Ile Arg Gly Lys Val Ala Val gatttgggca caa <210> 1014 <211> 34 <212> PRT <213> Corynebacteriun glutanicun <400> 1014 Leu Glu Leu Asn Lys Ala Ala Tyr Met Phe Glu 1 5 10.

Tyr Ser Phe Asp Asp Ile Thr Val Ser Gly Tyr Asp Pro His 25 Pro Leu Ile Arg Gly Lys Val Ala Val <210> <211> <212> <213> <220> <221> <222> <223> 1015 613

DNA

Corynebacteriun glutanicun

CDS

(101)..(613) RXN0 3171 <400> 1015 atactttgtt ggactggaaa agtggccgtt tggttccctc caagcccaaa ttcgcccgcg cggtcttctt ctgggcggca atgatttaac atgtgaagct atg gac atc acc Met Asp Ile Thr gtc aac cac cca ctc gtt gct agc cgc Val Asn His Pro Leu Val Ala Ser Arg acc ctg ttg cgc Thr Leu Leu Arg gac gag Asp Glu cgc agc gac Arg Ser Asp gca gct ttc cgt Ala Ala Phe Arg gca Ala gca gcc aac gac Ala Ala Asri Asp ctc ggc gcc Leu Gly Ala atg ctg atc tac gaa gca tcc Met Leu Ile Tyr Glu Ala Ser gat ctg gaa gtc gaa cac ttc gac Asp Leu Glu Val Glu His Phe Asp acc aaa Thr Lys acc ccc gtt gcc Thr Pro Val Ala gct gaa ggt act Ala Glu Gly Thr ctg aag cag cca Leu Lys Gin Pro ccc Pro atc atc gtt ccc atc atc cgt gca ggt Ile Ile Val Pro Ile Ile Arg Ala Gly ggc atg atc gac Gly Met Ile Asp gcg ctg tcg atg att ccg gat gca cag gtc ggc ttc att ggc ctt gcc 1400 WO 01/00843 Ala Leu Ser Met Ile cgc gat gag gaa acc Arg Asp Giu Giu Thr 105 cag gat cta agc aac Gin Asp Leu Ser Asn 120 acc ggc ggt tcc ctc Thr Gly Gly Ser Leu 135 gcc acc gac atc acc Ala Thr Asp Ile Thr 150 gac gca ttg gcg gaa Asp Ala Leu Ala Giu 170 PCTJIBOO/00923 L-u Ala Val c ca Pro ct t Leu cgc Arg gtt Val Gly tac Tyr gtc Val ctt Leu tct Ser 160 Phe Ile Gly c ca Pro gcc Ala ggc Gly gtg Val 165 <210> 1016 <211> 171 <212> PRT <213> Corynebacteriun glutamicun <400> 1016 Met Asp Ile Thr Ile Val Asn His Pro 1 Leu Asn Val Arg Gly Phe Leu Asp Leu 145 Arg Leu His Lys Ile Gly Ala 115 Met Asp Asp Gly Phe Gin Asp Leu 100 Leu Leu Arg Leu Ala Glu Val1 Pro Ile Thr Asn Leu Thr Ala Phe Ser Met Ile Asp Giu Pro His 140 Ile Ser Arg Arg Ala Arg Ala Pro Val 125 Ala Cys Ala Gin Pro Gly Val Asp Ala Leu Ala Giu Ser 1401 WO 01/00843 WO 0100843PCTIBOOIOO923 165 <210> 1017 <211> 613 <212> DNA <213> Corynebacteriun <220> <221> CDS <222> (101)..(613) <223> FRXA02857 <400> 1017 atactttgtt ggactggaaa glutani cuxn agtggccgtt tggttccctc caagcccaaa ttcgcccgcg cggtcttctt ctgggcggca atgatttaac atgtgaagct atg gac atc acc Met Asp Ile Thr gtc aac cac cca ctc gtt gct agc cgc Val Asn His Pro Leu Val Ala Ser Arg acc ctg ttg cgc Thr Leu Leu Arg gac gag Asp Glu cgc agc gac Arg Ser Asp atg ctg atc Met Leu Ile gca gct ttc cgt Ala Ala Phe Arg gca gcc aac gac Ala Ala Asn Asp ctc ggc gcc Leu Gly Ala cac ttc gac His Phe Asp tac gaa gca tcc Tyr Glu Ala Ser gat ctg gaa gtc Asp Leu Giu Val gaa Glu acc aaa Thr Lys acc ccc gtt gcc Thr Pro Val Ala gct gaa ggt act Ala Giu Gly Thr ctg aag cag cca Leu Lys Gln Pro atc atc gtt ccc Ile Ile Val Pro atc Ile atc cgt gca ggt Ile Arg Ala Gly ggc atg atc gac Gly Met Ile Asp gcg ctg tcg atg Ala Leu Ser Met ccg gat gca cag Pro Asp Ala Gin ggc ttc att ggc Gly Phe Ile Gly ctt gcc Leu Ala 100 cgc gat gag Arg Asp Glu cag gat cta Gin Asp Leu 120 acc cat gag cca Thr His Giu Pro cca tac ctt gag Pro Tyr Leu Giu gcg ctg cca Ala Leu Pro 115 atg ctg gcc Met Leu Ala agc aac cag cct Ser Asn Gin Pro ttc ctt gtc gat Phe Leu Val Asp acc ggc Thr Gly 135 ggt tcc ctc ctg Giy Ser Leu Leu c ac His 140 gcg atc cgc ctt Aia Ile Arg Leu gct gat cgt ggc Ala Asp Arg Gly acc gac atc acc Thr Asp Ilie Thr atc tgc atg gtt Ile Cys Met Val gcg cag cca ggt Ala Gln Pro Gly gac gca ttg gcg gaa tct 1402 WO 01/00843 Asp Ala Leu Ala Giu Ser 170 <210> 1018 <211> 171 <212> PRT <213> Corynebacteriux giutamicu <400> 1018 Met Asp Ile Thr Ile Val Asn His Pro PCTJIBOO/00923 1 Leu Asn Va1 Arg Gly Phe Leu Asp Leu 145 Leu Asp Glu Leu Met Ile Glu Pro 130 Ala Ser Leu Lys Ile Leu Asp Asp Gly 135 Thr Asp Ile Thr Ile Ser Glu Leu 120 Gly Asp Leu 10 Ala Glu Val Pro Ile Thr Asn Leu Thr Val Ala Ala Ala Ile 75 Pro His Gin Leu Ala 155 Leu Ala Leu Gly Gly Va1 Pro Leu Arg Val Thr Ala Glu Thr Leu Gly Tyr Val Leu Ser 160 Ala Gin Pro Giy Val Asp Ala Leu Ala Giu Ser 165 170 <210> 1019 <211> 678 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(655) <223> RXA02771 <400> 1019 gtttgtgata gatcgcacag tcggtaacgt tgttgttaat acagacctag ccggtatcgg atggaacatg gaccgttggt ccagaagtga ggaataagta gtg agc gaa caa gct 115 Val Ser Giu Gin Ala 1 1403 WO 01/00843 cta agc acc Leu Ser Thr tat gtg cag Tyr Val Gin ccg gtg ttg Pro Val Leu gct gaa gct Ala Glu Ala gcg cga gga Ala Arg Gly ggt gtg ctg Gly Val Leu acc cag gag Thr Gin Giu agt gaa gga Ser Glu Gly 120 gtg ctg gca Val Leu Ala 135 tcg tgt gac Ser Cys Asp 150 ggc ctc ggc Gly Leu Gly aga gat cct Arg Asp Pro ttc gac Phe Asp gat ttc Asp Phe ggc gat Gly Asp gca gaa Ala Glu ttc ctc Phe Leu gct atc Ala Ilie tat gaa Tyr Glu 105 atc gac Ile Asp acc ggc Thr Gly gga cat Gly His ggt agg Giy Arg 170 agg Arg cca Pro gca Al a aaa Lys ctc Leu 75 cgc Arg ctt Leu att Ile ggc Gly gtt Val1 155 gat gag Giu ggt Giy ttt Phe gca Ala gct Ala gga Gly ggc Gly 110 aaa Lys ggc Gly tat Tyr ggt ctg gac Leu Asp ctt ttt Leu Phe gcc gtg Ala Val atc atc Ile Ile gct tac Ala Tyr 80 ctc ccc Leu Pro gca gca Ala Ala atc gtt Ile Val gca cgt Ala Arg 145 ctt gcc Leu Ala 160 agg ccc Arg Pro PCTJIBOO/00923 :c cga 163 .ir Arg :c acc 211 au Thr atg 259 La Met :g gat 307 au Asp gc cta 355 Ly Leu :g gtg 403 al Val )o :g ccc 451 Bu Pro ic gat 499 3p Asp :t gaa 547 Le Glu -c cca 595 al Pro 165 gtc 643 al Val Asp Asn Leu Gly cag Gin 185 tagaaggatc gaaagaaagg cgg <210> 1020 <211> 185 <212> PRT <213> Corynebacteriun glutanicun <400> 1020 Val Ser Glu Gin Ala Leu Ser Thr Phe Asp Arg Ala Arg Glu Ala Leu 1 5 10 Asp Lys Lys Thr Arg Tyr Val Gin Asp Phe Pro Glu Lys Gly Val Leu 25 1404 WO 01/00843 Phe Giu As 3 Val Val As Ile Gly Gi Tyr Lys Le Pro Pro Pr Ala Leu Gi i1 Val Leu Il 130 Arg Lys Le 145 Ala Ile Gi Pro Val Ii Leu Ala Leu Giy Val 100 Leu Asp Ile Val Val 180 Thr Met Asp Leu Val Pro Asp Glu Pro 165 Val Pro Ala Ala 70 Gly Thr Ser Val Ser 150 Gly Arg Val Glu 55 Arg Val Gin Glu Leu 135 Cys Leu Asp Leu 40 Ala Gly Leu Glu Gly 120 Ala Asp Gly Pro Gly Al a Phe Al a Tyr 105 Ile Thr Gly Gly Gin 185 Ala Lys Leu 75 Arg Leu Ile Gly Val 155 Asp Glu Leu Gly Lys Glu Ala Thr 140 Ser Asn Ser Asn Ser Lys Tyr Gly 125 Leu Gly Leu PCTIBOOOO923 Phe Val Ala Ala Glu Ile Ala Val Ala Gly Lys Leu Gly Thr Ala 110 Lys Asn Ile Gly Ala Ala Tyr Val Leu 160 Gly Asp Arg 175 <210> 1021 <211> 723 <212> DNA <213> Corynebacterium glutanicun <220> <221> CDS <222> (101)..(700) <223> RXA01512 <400> 1021 gggtaaaagc gataatggaa ggttggaagt ggtgcggcaa tgattgacac ctgaatctac aacacaaggg gaacgcgacg gaa atg gcc gac cac aaa gat ctc aat gtt cca Glu Met Ala Asp His Lys Asp Leu Asn Val Pro 15 acc gac att gaa tca gta ttg atc agc gaa gag Thr Asp Ile Glu Ser Val Leu Ile Ser Glu Glu 30 atc gcc gaa atg gcc aag cgc gtc tcc gaa gag Ilie Ala Glu Met Ala Lys Arg Val Ser Glu Giu 45 gaa gac ctc atc ctg gtg tgc gtg ctc aag ggc 1405 agtggcaagc ttaagatcac atg agc aac aac gta 115 Met Ser Asn Asn Val 1 gcc aac cca tac ggc 163 Ala Asn Pro Tyr Gly aag ctc aag cag cgc 211 Lys Leu Lys Gin Arg ttc aaa gac gcc gaa 259 Phe Lys Asp Ala Giu gcg ttc tac ttc ctg 307 WO 01/00843 WO 0100843PCT/iBOOIOO923 Glu Asp Leu Ile Leu Val*Cys Val Leu Lys Gly Ala Phe 60 gca Ala gcg Ala atc Ile gtg Val aac Asn cgt Arg 150 ttt Phe gaa atg Met 75 gga Gly gac Asp gat Asp aac Asn ctg Leu 155 gag Glu c tc Leu aac Asn aag Lys tcc Ser ct Pro 140 acc Thr ttt Phe gac Asp tcc Ser gaa Glu gga Gly 125 aag Lys acc Thr gtt Val1 atc Ile acc Thr att Ile 110 ctg Leu tcc Ser aac Asn gtg Val1 gtg Val 190 Tyr Phe gag ttc Glu Phe gtg gtg Val Val 100 gtt ttg Val Leu 115 ctg atg Leu Met acc ttg Thr Leu Leu atg Met cgc Arg atc Ile cgc Arg ctg Leu gga Gly 165 gca Ala gtg Val gac Asp ga t Asp cc t Pro 195 att Ile ttc Phe 180 c ac His ctg cca tat Glu Arg Tyr Arg Asp Leu Pro Tyr 185 tac tcc gac tagtaatcaa aagtgcgaaa gag Tyr Ser Asp 200 <210> 1022 <211> 200 <212> PRT <213> Corynebacteriun giutarnicum <400> 1022 Met Ser Asn Asn Val Glu Met Ala Asp H: 1 5 Ala Asn Pro Tyr Gly Thr Asp Ile Glu Si 25 Lys Leu Lys Gin Arg Ile Ala Glu Met A: 40 Phe Lys Asp Ala Glu Glu Asp Leu Ile Li 55 Ala Phe Tyr Phe Leu Ala Asp Phe Ser X 70 Asp Leu Asn Val Pro Leu Ile Ser Glu Glu Arg Val Ser Glu Glu Cys Val Leu Lys Gly Leu Asp Ile Pro Thr 1406 WO 01/00843 Gin Ser Glu Phe Met Ala Val Ser Ser Tyr Gly 90 Ser Gly Val Val Arg Ile Leu Lys Asp Leu Asp 100 105 Arg Asp Val Leu Ile Val Glu Asp Ile Ile Asp 115 120 Ser Trp Leu Met Arg Asn Leu Lys Asn Arg Asn 130 135 Val Ile Thr Leu Leu Arg Lys Pro Glu Arg Leu 145 150 155 Met Phe Asp Ile Gly Phe Asp Ile Pro Asn Glu 165 170 Gly Leu Asp Phe Ala Giu Arg Tyr Arg Asp Leu 180 185 Leu Glu Pro His Val Tyr Ser Asp 195 200 <210> 1023 <211> 597 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS <222> (101)..(574) <223> RXA02031 <400> 1023 tgcttgggcg ttaacgattc tatatatact tccctagaaa ttgcagaacg ttgaagcatc attgactagg atatgtagac Thr Ile 110 Leu Ser Asn Val Val 190 PCTBOO/00923 Ser Ser Glu Gly Thr Leu Leu Asn Ile Asp 160 Gly Tyr 175 Gly Thr tcaagtgagc attcatctca atg aca gag gaa cgc gag Glu caa Gin cgt Arg aag Lys cac His tat Tyr gat Asp cta Leu gtg Val cca Pro gag Glu gac Asp atc Ile atc Ile atg Met 75 ttc gga Phe Gly cag cca Gin Pro ggc gca Gly Ala gtg gag Val Glu ctg cct Leu Pro Met Thr Giu Giu Arg 1 gca atg cgg gag ctg gcc Ala Met Arg Glu Leu Ala tgc gtg ctg tcc att gcg Cys Vai Leu Ser Ile Ala ggt tat gcg ctg ggt atc Gly Tyr Ala Leu Gly Ile tac acc gat att gga gag Tyr Thr Asp Ile Gly Glu act cca aaa gct gtt gat Thr Pro Lys Ala Vai Asp 80 115 163 211 259 307 355 1407 WO 01/00843 WO 0100843PCTfIBOO/00923 ctc tcg Leu Ser aag act Lys Thr gtg cgc Val Arg atg Met gag Glu 105 gca Ala gtc gct Val Ala gac ttc Asp Phe 110 cac aag His Lys 125 gac gat gtc gcg gat a4 Asp Asp Val Ala Asp TI 11 ctg ggt gac caa gtt gi Leu Gly Asp Gin Vai Vi 115 cca aac agt gtg ttt ai Pro Asn Ser Val Phe L, 130 tgg att aac ttc cca t4.

Trp Ile Asn Phe Pro T: 145 taatttttca cccgtgaaag gga Gly gaa Giu ccg Pro tc t Ser gag tat gtg tgg cgt gag act gat aag Giu Tyr Val Trp Arg Glu Thr Asp Lys 135 140 acc ctg cct cca gtg gag cct tct aag Thr Leu Pro Pro Val Glu Pro Ser Lys 150 155 tgC <210> 1024 <211> 158 <212> PRT <213> Corynebacteriun glutamicum <400> 1024 Met Thr Glu Glu Arg Glu Ile Leu Thr 1 met Val Thr Pro Val Asp Ser Asn 145 Arg Leu Aia Asp Lys Ala Gin Val 130 Phe Leu Ile Gly Gly Vai Thr 100 Val Lys Trp Ile Gly Val Glu Giy Leu Thr 120 Val Pro Ile Leu Ser Glu Met Glu 105 Ala Trp Pro Tyr 10 Asp Leu Val Pro Arg Leu Val Arg Val1 Giu Asp Ile Ile Met 75 Val Val Ile Glu Glu 155 Met Tyr Gly Asn Met Leu Arg Tyr Thr 140 Pro Phe Gin Gly Val1 Leu Val Asp His 125 Asp Ser Thr Asp Leu Phe Pro Asp Leu Pro Trp, Ala Cys Gly Tyr Thr Asp Gly Asn Ile 1408 WO 01/00843 WO 0100843PCTIiBOO/00923 <210> <211> <212> <213> <220> <221> <222> <223> 1025 753

DNA

Corynebacterium giutanicun

CDS

(101)..(730) RXA00981 <400> 1025 aaccaatggc tgggtactga tgtggtgatc agtgcccagt ttcttctttc tactagtgtc ggatagaagt acccccagtc cagaatgaag gtcaccacca atg tca gag aat met Ser Glu Asn 1 cca gcg ccc gag Pro Ala Pro Glu aa t Asn ctc ctg gac gcc Leu Leu Asp Ala aga att cag atg Arg Ile Gin Met atc aag Ile Lys aac ttc cgc Asn Phe Arg att gat gag Ile Asp Glu gaa tta acg ggg Giu Leu Thr Giy atg ctc aac tac Met Leu Asn Tyr caa ttt ggc Gin Phe Gly atc ctg acc aag Ile Leu Thr Lys atc aac Ile Asn 45 atc ctg aaa act gaa ttc agc Ile Leu Lys Thr Glu Phe Ser cag ctg Gin *Leu cac gaa tac gca His Giu Tyr Ala cct Pro 60 atc gag cac gta tct tca cga ttg aag Ile Giu His Val Ser Ser Arg Leu Lys aca Thr cca gaa agc atc Pro Giu Ser Ilie aaa aag gtc atc Lys Lys Val Ile cga Arg aaa gga gac gag Lys Gly Asp Glu tcc ctc gca gct Ser Leu Ala Ala aaa gac aca gtg Lys Asp Thr Vai gat atc gca ggc Asp Ilie Ala Gly att cga Ile Arg 100 atc gtc tgc Ile Val Cys acc aac caa Thr Asn Gin 120 agt Ser 105 ttc ctc aaa gat gcc tac gca atc gcc Phe Leu Lys Asp Ala Tyr Ala Ile Ala 110 gat atg ctg Asp Met Leu 115 tac atc gct Tyr Ile Ala aaa gac gtc acg Lys Asp Val Thr gtc Val 125 atc gag gcc aaa Ile Giu Ala Lys aac cca Asn Pro 135 aag ccg aac ggc Lys Pro Asn Gly tac Tyr 140 aag agt ttg cac Lys Ser Leu His ctt Leu 145 atc ctc caa gtg Ile Leu Gin Val cct gtc ttc ctg tct aac tcc gtg gaa aag Pro Vai Phe Leu Ser Asn Ser Val Giu Lys gtc Vai 160 aat gtt gaa gtc Asn Val Glu Val atc cgc acc att Ile Arg Thr Ilie atg gac ttc tgg Met Asp Phe Trp gca Aila 175 agc ctc gag cac Ser Leu Glu His aaa atc Lye Ile 180 1409 WO 01/00843 WO 0100843PCTIiBOO/00923 tac tac aaa ttt gaa caa gaa gtt cct cag tca atc ctt gat gag ctc Tyr Tyr Lys Phe Glu Gin Glu Val Pro Gin Ser Ilie Leu Asp Giu Leu 185 190 195 agt gaa gat gga aag aat cca cgg gga agt gaa gtc act taaacctcca Ser Glu Asp Gly Lys Asn Pro Arg Giy Ser Giu Val Thr 200 205 210 gttgaaacca ctg <210> 1026 <211> 210 <212> PRT <213> Corynebacterium giutamicum <400> 1026 Met Ser Glu Asn Leu Pro Ala Pro Glu Asn Leu Leu Asp Ala Giu Arg 691 740 753 1 Ile Asn Lys Ser Lys Ile Ile Lys Leu 145 Asn Leu Met Gin Giu Arg Asp Giy Asp 115 Tyr Leu Giu His Ilie Phe Phe Leu Giu Ile 100 Met Ile Gin Val Lys 180 Lys Gly Ser Lys Leu Arg Leu Ala Vai Gin 165 Ile Asn Ile Gin Thr 70 Ser Ile Thr Asn Pro 150 Ile Tyr Leu Thr Ala Val Lys Leu Vai Giy Asn 155 Met Gin Thr Lys Pro Lys Asp Lys Thr Tyr 140 Ser' Asp Glu Gly Ile Ile Lys Thr Asp Val 125 Lys Val1 Phe Val Phe Asn Giu Val Val Ala 110 Ile Ser Giu Trp Pro 190 Ile Leu Asp 195 Vai Thr 210 <210> 1027 Giu Leu Ser Giu Asp 200 Gly Lys Asn Pro Giy Ser Giu 1410 WO 01/00843 WO 0100843PCTIIBOO/00923 <211> 1158 <212> DNA <213> Corynebacteriun <220> <221> CDS <222> (101)..(1135) <223> RXN02772 <400> 1027 tgaggtccca ggcctcggcg gi utami cuin gtagggataa tcttggtgat aggcccgtca ttgtggtcag agatcctcag tagaaggatc gaaagaaagg cggcaggaaa atg agt ctg gag Met Ser Leu Giu 1 aac aca caa aaa Asn Thr Gin Lys tcc atg ggt gtg Ser Met Gly Val agc atg tca Ser Met Ser gcc cgc agc Ala Arg Ser ccg ctg ctg Pro Leu Leu ctc aca gga aac cgc gtt cgc acc aac cct Leu Thr Giy Asn Arg Val Arg Thr Asn Pro 30 agc atc cac cgg caa ttt cac cca cgc gcc Ser Ile His Arg Gin Phe His Pro Arg Ala gcc agg ctt Ala Arg Leu gtg ctg gat Val Leu Asp gac gta caa Asp Vai Gin gtg ttg Vai Leu gaa cgt gca tat Giu Arg Ala Tyr gac Asp acc gcg gaa cgt Thr Ala Giu Arg cat gat ggt gtg His Asp Gly Val att Ilie cga aaa tcg ggc Arg Lys Ser Gly ccg tat att acc cac ccg ttg gct gtc Pro Tyr Ile Thr His Pro Leu Ala Val acc atc gcc gcg Thr Ile Ala Ala atc ggc atg gac acc acc acg ctc gtc Ile Gly Met Asp Thr Thr Thr Leu Val gca gcc Ala Ala 100 ttg ttg cat Leu Leu His acc cga gat Thr Arg Asp 120 gac Asp 105 acg gtg gaa gac Thr Val Glu Asp ac c Thr 110 gac tac tct ttg Asp Tyr Ser Leu gac gat ctc Asp Asp Leu 115 ggt gtc acc Gly Val Thr 451 499 ttc gga gaa gaa Phe Gly Giu Giu gcc agg ctt gtc Ala Arg Leu Val aag ctc Lys Leu 135 gac aaa gtc gca Asp Lys Val Ala ggt gct gcc gcg gag gcc gaa acg att Gly Ala Ala Ala Giu Ala Glu Thr Ile 145 cgc Arg 150 aaa atg atc gtc Lys Met Ilie Val atg agc cag gac Met Ser Gin Asp ccc Pro 160 cgc gtg ctg gtg att Arg Val Leu Val Ile 165 595 643 aaa gtg gcc gac Lys Val Ala Asp ttg cac aat atg Leu His Asn Met cgc Arg 175 acc atg cgg ttc Thr Met Arg Phe ctg ccg Leu Pro 180 ccg gaa aag caa gct aaa aaa gca cgc caa acc ctt gaa gtg att gct 1411 WO 01/00843 Pro Giu Lys cct ttg gca Pro Leu Ala 200 gat cta tcc Asp Leu Ser 215 cgt ctt gtt Arg Leu Val 230 att att gat Ile Ile Asp gaa gtg ctt Giu Val Leu atc gtt cgc Ile Val Arg 280 cgc atc ctg 1027 Arg Ile Leu 295 cgt gca ctc 1075 Arg Ala Leu 310 ttc agc ccc 1123 Phe Ser Pro ggg acc tgg 1158 Gly Thr Trp Gin Ala 185 cac cgc His Arg ttt gcc Phe Ala gcc gac Ala Asp caa gtc Gin Vai 250 ggt cga Gly Arg 265 ggt cgt Gly Arg gta gac Val Asp cct gtt Pro Val gcg ctt Aia Leu 330 cgg taaC Arg 345 Lys Lys Ala Arg Gin Tnlr 190 ctg ggc atg gcc agc gtg Leu Gly Met Aia Ser Val 205 att ttg tac ccc aag aag Ile Leu Tyr Pro Lys Lys 220 cgc gcg ccc tct aga gac Arg Aia Pro Ser Arg Asp 235 240 acc ggt ggc ttg cgc gaa Thr Gly Gly Leu Arg Giu 255 cca aag cac tac tgg tct Pro Lys His Tyr Trp Ser 270 gat ttt gac gat att ttt Asp Phe Asp Asp Ile Phe 285 aac gtg aac aac tgt gta Asn Val Asn Asn Cys Vai 300 caa tgc tct gcc tgg ccg Gin Cys Ser Aia Trp Pro 315 320 cgg tgt cta cca atc cct Arg Cys Leu Pro Ile Pro 335.

~cctctg gaagttcagg cac Leu aaa Lys tac Tyr 225 cgg Arg aac Asn atc Ile gat Asp cgc Arg 305 at t Ile gca Ala Giu tgg Trp 210 gaa Giu tac Tyr aac Asn tat Tyr ctt Leu 290 cgc Arg caa Gin cac His PCTIiBOO/00923 Ile Ala ttg gaa 739 Leu Giu atc gtg 787 Ile Val aaa gaa 835 Lys Giu 245 gcg gca 883 Ala Ala 260 aag atg 931 Lys Met ggc atc 979 Gly Ile cgg tgt Arg Cys cta tat Leu Tyr 325 cgt gat Arg Asp 340 <210> 1028 <211> 345 <212> PRT <213> Corynebacterium giutamicum <400> 1028 Met Ser Leu Giu Arg Asn Thr Gin Lys Ser Ser Met Giy Val Arg Ser 1 5 10 Met Ser Ala Arg Leu Ala Arg Ser Leu Thr Giy Asn Arg Vai Arg Thr 25 Asn Pro Val Leu Asp Pro Leu Leu Ser Ile His Arg Gin Phe His Pro 1412 WO 01/00843 WO 0100843PCTIIB0O/00923 Arg Leu Pro Thr Ser Val Glu 145 Arg Met Leu Lys Tyr 225 Arg Asn Ile Asp Arg 305 Ile Ala Asp Asp Ala Val Asp 115 Gly Giu Leu Phe Val1 195 Giu Giu Leu Ile Gin 275 Val His Arg His Val Giy Val Ala 100 Asp Val Thr Val1 Leu 180 Ile Leu Ile Lys Ala 260 Lys Gly Arg Leu Arg 340 Gin Val Ala Ala Leu Thr Ile Ile 165 Pro Ala Giu Val Giu 245 Ala Met Ile Cys Tyr 325 Asp Val Ile 70 Thr Leu Thr Lys Arg 150 Lys Pro Pro Asp Arg 230 Ile Giu Ile Arg Arg 310 Phe Giy Leu Arg Ile Leu Arg Leu 135 Lys Val Glu Leu Leu 215 Leu Ile Vai Val Ile 295 Ala Ser Thr Giu Lys Ala His Asp 120 Asp Met Ala Lys Ala 200 Ser Val Asp Leu Arg 280 Leu Leu Pro Trp Ala Tyr Asp Thr Ala Giu Gly Giu 90 Thr Gly Val Val Arg 170 Ala Arg Ala Asp Val1 250 Arg Arg Asp Val1 Asp 75 Ile Val Giu Ala Ala 155 Leu Lys Leu Ile Arg 235 Thr Pro Asp Asn Gin 315 Arg Pro Gly Giu Glu Leu 140 Met His Lys Giy Leu 220 Al a Gly Lys Phe Val 300 Cys Cys Tyr Met Asp Val 125 Giy Ser Asn Ala Met 205 Tyr Pro Gly His Asp 285 Asn Ser Leu Ile Asp Thr 110 Ala Ala Gin Met Arg 190 Ala Pro Ser Leu Tyr 270 Asp Asn Ala Pro Thr T1jr Asp Arg Ala Asp Arg 175 Gin Ser Lys Arg Arg 255 Trp Ile Cys Trp, Ile 335 Arg His Thr Tyr Leu Ala Pro 160 Thr Thr Val Lys Asp 240 Glu Ser Phe Val Pro 320 Pro Ala Leu 330 Arg 345 <210> 1029 1413 WO 01/00843 <211> 903 <212> DNA <213> Corynebacterium giutanicum <220> <221> CDS <222> (101)..(880) <223> FRXA02772 <400> 1029 cattgtggtc agagatcctc agtagaagga tcgaaagaaa PCTIBOO/00923 ggcggcagga aaatgagtct ggagcgcaac acacaaaaat cttccatggg tgtgcgaagc atg tca gcc agg ctt Met Ser Ala Arg Leu gcc Ala ccg Pro gtg Val att Ile acc Thr ttg Leu ac c Thr aag Lys cgc Arg aaa Lys 150 cc g Pro cc t cgc Arg ctg Leu ttg Leu cga Arg atc Ile ttg Leu cga Arg ctc Leu aaa Lys 135 gtg Val gaa Glu ttg cgc Arg caa Gin acc Thr tat Tyr atg Met gac Asp gtt Vai ggt Gly 125 agc Ser aat Asn gca Ala atg cgc Arg cac His gaa Giu acc Thr acc Thr gac Asp agg Arg gcc Ala gac Asp cgc Arg caa Gin 175 agc acc Thr cca Pro cgt Arg cac His ac c Thr 80 tac Tyr ctt Leu gcg Aia ccc.

Pro ac c Thr 160 acc Thr gtg 1 aac Asn cgc Arg ctt Leu ccg Pro 65 acg Thr tc t Ser gtc Vai gag Glu cgc Arg 145 atg Met ctt Leu aaa ctg Leu 20 gta Vai ggt Giy gtc Val gca Ala gat Asp 100 gtc Val acg Thr gtg Val1 ctg Leu att Ile 180 ttg gat Asp caa Gin gtg Val gcc Ala gc-c Ala ctc Leu ac c Thr att Ile att Ile ccg Pro 165 gct Ala gaa 115 163 211 259 307 355 403 451 499 547 595 643 691 1414 WO 01/00843 Pro Leu Ala His Arg Leu Gly Met Ala Ser Vai 185 190 gat cta tcc ttt gcc att ttg tac ccc aag aag Asp Leu Ser Phe Ala Ile Leu Tyr Pro Lys Lys 200 205 cgt ctt gtt gcc gac cgc gcg ccc tct aga gac Arg Leu Val Ala Asp Arg Ala Pro Ser Arg Asp 215 220 att att gat caa gtc acc ggt ggc ttg cgc gaa Ile Ile Asp Gln Val Thr Gly Gly Leu Arg Giu 230 235 240 gaa gtg ctt ggt cgn ccn aag cac tct ggt ctt Glu Val Leu Gly Arg Pro Lys His Ser Gly Leu 250 255 tgatcgttcg cggtcgtgat ttt <210> 1030 <211> 260 <212> PRT <213> Corynebacterium glutamicun Trp, gaa Giu 210 tac Tyr aac Asn ttc Phe Li a' PCTLBOO/00923 eu Glu tc gtg 739 le Val aaa Lys gcg Ala aga Arg 260 gaa Giu gca Ala 245 <400> 1030 Met Ser Ala 1 Asn Pro Val Arg Ala Asp Leu His Asp Pro Leu Ala Thr Leu Val Ser Leu Asp Val Asp Gly 115 Giu Ala Glu 130 Arg Val Leu 145 Met Arg Phe.

Arg Leu Ala Arg Ser Leu Leu Leu Arg 55 Ile Leu Arg Leu Lys 135 Val Glu Leu Giu 40 Lys Ala His Asp Asp 120 Met Ala Lys Thr 10 Ile Ala Gly Glu Thr Gly Val Val Arg Ala 170 Gly His Tyr Asp Ile 75 Val Giu Ala Ala Leu 155 Lys Asn Arg Asp Pro Gly Giu Glu Leu Met 140 His Lys Ar'g Gin Thr Tyr Met Asp Val Gly 125 Ser Asn Ala Arg His Giu Thr Thr Asp Arg Ala Asp Arg Gin 175 Thr Pro Arg His Thr Tyr Leu Ala Pro Thr 160 Thr 1415 WO 01/00843 Leu Giu Va Lys Trp GI 19 Tyr Glu GI 210 Arg Tyr Le 225 Asn Asn Ii Ser Phe Ly Pro Leu Ala His 185 Asp Leu Ser Phe 200 Arg Leu Val Ala 215 Ile Ilie Asp Gin 230 Glu Val Leu Gly Arg Ala Asp Val Arg 250 Leu Gly Met Ala 190 Ile Leu Tyr Pro 205 Arg Ala Pro Ser 220 Thr Gly Gly Leu 235 Pro Lys His Ser PCTIIBOO/00923 Ser Val Lys Lys Arg Asp Arg Glu 240 Gly Leu 255 <210> 1031 <211> 262 <212> DNA <213> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(262) <223> FR.XA02773 <400> 1031 tcaaagaaat tattgatcaa gtcaccggtg gcttgcgcga tgcttggtcg nccnaagcac tctggtcttt ctttcaaaag cgt Arg gac Asp gtn Val ctt Leu ttt gac gat att ttt Phe Asp Asp Ilie Phe gtg aac aac tgg gta Val Asn Asn Trp Val tgc tct gnc tgg ncg Cys Ser Xaa Trp Xaa gat Asp cgc Arg ntt Xaa 45 ctt gtt ggc Leu Val Gly 15 cgc cnt cgg Arg Xaa Arg 30 caa aga cta Gin Arg Leu aaacaacatc gcggcagaag atg atc gtt cgc ggt Met Ile Val Arg Gly 1 atc cgc ntc ctg gna Ile Arg Xaa Leu Xaa tgt cgt gca ctc ccn Cys Arg Ala Leu Pro tat ttc agc ccc gcg Tyr Phe Ser Pro Ala <210> 1032 <211> 54 <212> PRT <213> Corynebacteriun giutawicun <400> 1032 Met Ile Val Arg Gly Arg Asp Phe Asp Asp Ile Phe Asp Leu Val Gly 1 5 10 1416 WO 01/00843 WO 0100843PCTIEiBOO/00923 Ile Arg Xaa Leu Xaa Asp Asn Val Asn Asn Trp Val Arg Arg Xaa Arg 25 Cys Arg Ala Leu Pro Val Gin Cys Ser Xaa Trp Xaa Xaa Gin Arg Leu 40 Tyr Phe Ser Pro Ala Leu <210> 1033 <211> 654 <212> DNA <213> Corynebacteriun glutamicum <220> <221> CDS <222> (101)..(631) <223> RXA01835 <400> 1033 tcaacatcta ttcctcctgc gatttgcatg ggatatatat taaaaattct agccgaaac It ttcctgcgtg aatacacttt ccccgcgcct tcgcaaagct tgg Trp, agc Ser gaa Giu ccc Pro gtg Val aaa Lys tta Leu tcc Ser cac His g tg Val gcc Ala tct Ser gaa Glu 75 gc t Ala atc Ile ctt Leu gta Val a tg Met ggg Gly gcc Ala gag Glu tac Tyr tcc Ser gaa Giu cgc Arg tac Tyr ctg Leu caa Gin c tc Leu ctc Leu acc Thr ata Ile 125 aaa Lys ttg Leu ctc Leu ctt Leu acc Thr c tg Leu gca Ala 110 cac His ggc Gly c tg Leu cac His gaa Giu aaa Lys cac His gat Asp ggt Gly atg aat act gcc gcg 115 Met Asn Thr Ala Ala 1 gga att ccg tat gtc 163 Gly Ile Pro Tyr Val agc gtc act aat gat 211 Ser Val Thr Asn Asp acc ctc gaa gac gta 259 Thr Leu Glu Asp Val gat ttt ggt ccg cgg 307 Asp Phe Gly Pro Arg ccc tta aaa agc tgg 355 Pro Leu Lys Ser Trp agcgca ggt gcc agc 403 Ser Ala Gly Ala Ser 100 ctg cat aat ctc atg 451 Leu His Asn Leu Met 115 gat tta tgg caa cgc 499 Asp Leu Trp, Gin Arg 130 tat agc gag gtt tat 547 ttt aac gct ggc aaa gag cag caa atc tgg tgg 1417 WO 01/00843 Phe Asn Ala Gly Lys Giu Gin Gin Ile 135 140 cag ata tct ctc cag cgc tta ggg ttc Gin Ilie Ser Leu Gin Arg Leu Gly Phe 150 155 ggg ttg tgc gtc gaa aag ctc tta aag Gly Leu Cys Val Glu Lys Leu Leu Lys 170 gcggcgtcga taa <210> 1034 <211> i77 <212> PRT <213> Corynebacteriun giutarnicum <400> 1034 Met Asn Thr Aia Ala Trp Ala His Arg PCT/IIBOO/00923 Trp Trp Tyr Ser Glu Val Tyr 145 aat gag ttg aat aaa caa ctg Asn Giu Leu Asn Lys Gin Leu 160 165 caa agc gcc taggcgctcg Gin Ser Ala 175 1 Gly Ser Thr Asp Pro Ser Leu Asp Tyr 145 Leu His Asp Glu Arg Al a Giu Ile Asn 135 Ile Leu Leu Val Glu Glu Arg Ala Leu 120 Ala Ser Cys Lys Leu Leu Leu Thr Leu Ala 110 His Ile Phe Lys Gly Leu His Glu Lys His Asp Gly Trp Asn Gin 175 Gly Ala Asp Ala Gin Leu Lys Giu Trp Glu 160 Ser Ala <210> 1035 <211> 1395 <212> DNA <213> Corynebacterium glutarnicum 1418 WO 01/00843 WO 0100843PCT/EBOO/00923 <220> <221> CDS <222> (101)..(1372) <223> RXA01483 <400> 1035 gggtgcccga taaggtgaag catctttgcg cgcaatccaa gcgtgcacgc catgagcaac ttcggaggct gaaaaagtag ggtctgctgc gcccttcgcg atg tac ccc tat tcc 11! Met Tyr Pro Tyr Ser 163 gac gca gac gct ttt cga cgc cag cct gag cgc gcc aag tcc Asp Ala Asp Ala Phe Arg Arg Gin Pro Giu Arg Ala Lys Ser agc caa Ser Gin ctg cgt acc Leu Arg Thr gct cgc gtg Ala Arg Val agc gcc Ser Ala gta gac acc Val Asp Thr agc gcg ttc gcc Ser Ala Phe Ala cgc gac cgg Arg Asp Arg gat aaa acc Asp Lys Thr ctg cat tct gct gct ctt cga cgc ctc Leu His Ser Ala Ala Leu Arg Arg Leu caa gtg Gin Val gtt ggc ccc aat Val Gly Pro Asn gat ggt gat act ccg cgc acc cgg ctg acg Asp Gly Asp Thr Pro Arg Thr Arg Leu Thr 60 cac His tct ttg gaa gta gct caa att gca cgg Ser Leu Glu Val Ala Gin Ile Ala Arg atc gga gct gga Ile Gly Ala Gly gat ttg gat cct Asp Leu Asp Pro ctg tgc gat ctg gca ggg ctg tgc cat Leu Cys Asp Leu Ala Gly Leu Cys His gac att Asp Ile 100 ggg cat ccg Gly His Pro gct gcg gcc Ala Ala Ala 120 ccg Pro 105 tat gga cac aac Tyr Gly His Asn ggt Gly 110 gaa aac gcg ttg Giu Asn Ala Leu aat gaa gtt Asn Giu Val 115 ttg cgt att Leu Arg Ile tgt gga gga ttt Cys Gly Gly Phe gag Glu 125 ggc aac gcc caa Gly Asn Ala Gin ctc acg Leu Thr 135 cgt ctg gag cca Arg Leu Giu Pro aaa Lys 140 att gtc tct gat Ile Val Ser Asp gag Giu 145 ggg gag agc ttt Gly Giu Ser Phe ggg Gly 150 ctg aac ttg tcg Leu Asn Leu Ser gct gct ctt gat Ala Ala Leu Asp gca tgt aag tat Ala Cys Lys Tyr tgg gct aaa aca Trp Ala Lys Thr aa t Asn 170 gcg gat ggc agt Ala Asp Giy Ser gtc Val 175 aat aag aaa tac Asn Lys Lys Tyr agt gct Ser Ala 180 tat gac gag Tyr Asp Giu gac Asp 185 gca gaa atc ctc Ala Giu Ile Leu gct Ala 190 tgg att aga caa Trp Ile Arg Gin ggc cat gag Gly His Giu 195 1419 WO 01/00843 gat ctt cgc Asp Leu Arg 200 att gcc tac Ilie Ala Tyr PCTIBOOIOO923 cca ccg atc gaa Pro Pro Ile Glu cag gtc atg gac Gin Val Met Asp ttt Phe 210 gta Val tct gat gac Ser Asp Asp tcc ggg cgt Ser Gly Arg tcg gtg cac Ser Val His gaa gac ggt Giu Asp Gly atc Ilie 230 gac Asp tta aaa gtg Leu Lys Val ctg Leu 235 gc t Ala gac ctg gtt Asp Leu Val gca gct ttg Ala Ala Leu 739 .787 835 883 931 979 aaa gga gca Lys Giy Ala gct Ala 250 ttg Leu ttc gga ggt Phe Gly Gly gca gag ctc Ala Giu Leu atc gag Ile Giu 260 ggt gca gca Gly Ala Ala ttt gat ttc Phe Asp Phe 280 cgg gaa ctt Arg Glu Leu gtg gct gcc Val Ala Ala gct gcg gat Ala Ala Asp 275 ctg cgt tcc Leu Arg Ser gct gcg ctg aag Ala Ala Leu Lys gcg atg act tca Ala Met Thr Ser 290 tca aca aag aaa Ser Thr Lys Lys gaa cta 1027 Giu Leu 295 aca cac 1075 Thr His 310 cca gaa 1123 Pro Giu ctc tac 1171 Leu Tyr cgg gat 1219 Arg Asp gga tcg 1267 Gly Ser 375 gaa tcg 1315 Glu Ser 390 gag tct 1363 gtg gga aga tac gtt Val Gly Arg Tyr Val 300 gct ggc att gat gtg Ala Gly Ile Asp Val ggc tct acc atc Gly Ser Thr Ile gga cgc atg cac Gly Arg Met His gag Giu 305 aca gcg gcc Thr Ala Ala 330 gtg atg gat Val Met Asp 315 agt Ser gac Asp gaa gta aaa ctg Giu Val Lys Leu 335 cca ggg cac ctt Pro Gly His Leu 350 ggc gat ttg atc att Gly Asp Leu Ile Ile 325 aaa acg tta gcg gtt Lys Thr Leu Ala Val 340 cgc caa aac agg caa Arg Gin Asn Arg Gin gcg Al a 345 cgt atc Arg Ile 355 ttc cgg gtt ttt Phe Arg Val Phe 365 ccg atg tat cgc Pro Met Tyr Arg gac tac ctg gtg ctg ggg gct ccg Asp Tyr Leu Val Leu Gly Ala Pro 370 cag tgg ttt att gaa gcg gat tca Gin Trp Phe Ilie Giu Ala Asp Ser 385 ga t Asp 380 gaa cag atc cgt Giu Gin Ilie Arg 395 cgt ctg gaa cgc gtg att gtt gat cag Val Ile Val Asp Gin 400 ctt gcc cgg aat gct att gcg tcg atg acg Ilie Ala Ser Met Thr 405 gct gac atc tca gga.

1420 WO 01/00843 PCT/IBOOIOO923 Glu Ser Arg Leu Glu Arg Leu Ala Arg Asn Ala Ala Asp Ile Ser Gly 410 415 420 ttt ttg gga taattggtta gagcagcagt aag 1395 Phe Leu Gly <210> 1036 <211> 424 <212> PRT <213> Corynebacteriun glutamicun <400> 1036 Met Tyr Pro Tyr Ser Asp Ala Asp Ala Phe Arg Arg Gin Pro Glu Arg 1 5 10 Ala Lys Ser Ser Gin Leu Arg Thr Ser Ala Val Asp Thr Arg Ser Ala 25 Phe Ala Arg Asp Arg Ala Arg Val Leu His Ser Ala Ala Leu Arg Arg 40 Leu Ala Asp Lys Thr Gln Val Val Gly Pro Asn Asp Gly Asp Thr Pro 55 Arg Thr Arg Leu Thr His Ser Leu Glu Val Ala Gln Ile Ala Arg Gly 70 75 Ile Gly Ala Gly Leu Asp Leu Asp Pro Asp Leu Cys Asp Leu Ala Gly 90 Leu Cys His Asp Ile Gly His Pro Pro Tyr Gly His Asn Gly Glu Asn 100 105 110 Ala Leu Asn Glu Val Ala Ala Ala Cys Gly Gly Phe Glu Gly Asn Ala 115 120 125 Gln Thr Leu Arg Ile Leu Thr Arg Leu Glu Pro Lys Ile Val Ser Asp 130 135 140 Glu Gly Giu Ser Phe Gly Leu Asn Leu Ser Arg Ala Ala Leu Asp Ala 145 150 155 160 Ala Cys Lys Tyr Pro Trp Ala Lys Thr Asn Ala Asp Gly Ser Val Asn 165 170 175 Lys Lys Tyr Ser Ala Tyr Asp Giu Asp Ala Glu Ile Leu Ala Trp Ile 180 185 190 Arg Gin Gly His Glu Asp Leu Arg Pro Pro Ile Glu Ala Gln Val Met 195 200 205 Asp Phe Ser Asp Asp Ile Ala Tyr Ser Val His Asp Val Glu Asp Gly 210 215 220 Ile Val Ser Gly Arg Ile Asp Leu Lys Val Leu Trp Asp Leu Val Glu 225 230 235 240 Leu Ala Ala Leu Ala Asp Lys Gly Ala Ala Ala Phe Gly Gly Ser Pro 1421 WO 01/00843 WO 0100843PCTflIBOO/00923 245 250 255 Ala Glu Leu Ile Giu Gly Ala Ala Ser Leu Arg Giu Leu Pro Val Val 260 265 270 Ala Ala Ala Ala Asp Phe Asp Phe Ser Leu Arg Ser Tyr Ala Ala Leu 275 280 285 Lys Ala Met Thr Ser Giu Leu Val Gly Arg Tyr Val Gly Ser Thr Ile 290 295 300 Giu Ser Thr Lys Lys Thr His Ala Gly Ile Asp Val Giy Arg Met His 305 310 315 320 Gly Asp Leu Ile Ilie Pro Giu Thr Ala Ala Ser Giu Val Lys Leu Leu 325 330 335 Lys Thr Leu Ala Val Leu Tyr Val Met Asp Asp Pro Gly His Leu Ala 340 345 350 Arg Gin Asn Arg Gin Arg Asp Arg Ile Phe Arg Val Phe Asp Tyr Leu 355 360 365 Val Leu Gly Ala Pro Gly Ser Leu Asp Pro Met Tyr Arg Gin Trp, Phe 370 375 380 Ile Giu Ala Asp Ser Giu Ser Giu Gin Ile Arg Val Ile Val Asp Gin 385 390 395 400 Ile Ala Ser Met Thr Giu Ser Arg Leu Giu Arg Leu Ala Arg-Asn Ala 405 410 415 Ala Asp Ilie Ser Gly Phe Leu Gly 420 <210> 1037 <211> 1131 <212> DNA <213> Corynebacteriun giutanicun <22 0> <221> CDS <222> (101)..(1108) <223> RXN01027 <400> 1037 aatagatgga agtagttttt cattcactta tgtgcgcgtt tttaatctgg tttctaccaa gaactgtgtg caccacaacg cggaaggtga atcgcaccca atg gca aat aag aac 115 Met Ala Asn Lys Asn 1 aat aag cct cat gag gtg gac aaa gac caa gat tca gcc atg ctg atc 163 Asn Lys Pro His Giu Val Asp Lys Asp Gin Asp Ser Ala Met Leu Ile 15 aac ggt cgc ctg caa cag atc ccg gcg cgt ccc act gag gaa ttC acc 211 Asn Gly Arg Leu Gin Gin Ile Pro Ala Arg Pro Thr Giu Giu Phe Thr 30 1422 WO 01/00843 cgc cca act Arg Pro Thr PCT/IBOO/00923 ctt gca gca ggt Leu Ala Ala Gly gta ctg tgg cgc Val Leu Trp Arg gac atc acc Asp Ile Thr aac ccg Asn Pro gac agc atc gag Asp Ser Ilie Glu gct gtc atc cac Ala Val Ile His ccg cac tat gat Pro His Tyr Asp gac Asp aca Thr tgg tcc ctg gcc Trp, Ser Leu Ala acc gcg gcc cgt Thr Ala Ala Arg aag Lys 75 ggc aaa gtc gat Gly Lys Val Asp ccc Pro ggc gag tct att Gly Giu Ser Ile gaa atc ctt gaa Giu Ile Leu Glu act ggc tac gac Thr Gly Tyr Asp atc cgt Ilie Arg 100 ctg ggc aag Leu Gly Lys aaa gtg gtc Lys Val Val 120 ctg Leu 105 atc ggc aag gtt Ile Gly Lys Val tac cct gtg ctc Tyr Pro Val Leu gac cga acc Asp Arg Thr 115 gag ttt gtc Giu Phe Val tac tac tgg act Tyr Tyr Trp Thr gcc Ala 125 cag gtt ctt ggt Gin Vai Leu Gly ccc aac Pro Asn 135 gat gaa gtt gat Asp Glu Vai Asp gaa Giu 140 atc cgt tgg ctg tct gtt gat gaa gca Ile Arg Trp Leu Ser Val Asp Glu Aia 145 tgc Cys 150 gag ttg ctc agc Glu Leu Leu Ser tac Tyr 155 caa gta gat acc Gin Val Asp Thr gaa Glu 160 gtt ctg gcc aag Vai Leu Ala Lys gca, Ala 165 gca aag cgt ttc Ala Lys Arg Phe act cct tcc acc Thr Pro Ser Thr cgg g'tg ctg tat Arg Vai Leu Tyr gtt cgc Val Arg 180 cat gct cat gca cat ggt cgc caa His Ala His Ala His Gly Arg Gin 185 tgg ggt ggc gac Trp Giy Gly Asp gac aat aag Asp Asn Lys 195 ctc gta ccc Leu Val Pro cgc cca ttg Arg Pro Leu 200 gac aaa aag ggg Asp Lys Lys Gly cgt Arg 205 cga caa gca gaa Arg Gin Ala Giu atg ttg ttg ccc ttc aaa Met Leu Leu Pro Phe Lys 215 ccc Pro 220 acc gca att tac Thr Ala Ilie Tyr tcg Ser 225 gcg gtg ccc gat Ala Val Pro Asp cgc Arg 230 tgc caa gcc acc Cys Gin Ala Thr gcg Ala 235 ctc ccc ctt gcc Leu Pro Leu Ala gat Asp 240 gag ctc ggc ctc Giu Leu Gly Leu gac Asp 245 gtg tcc gtc aac Val Ser Val Asn ctg ttc ggc gac Leu Phe Gly Asp gac Asp 255 gcc tgg gaa acc Ala Trp, Giu Thr gat ccc Asp Pro 260 gag gcc tgc Giu Ala Cys aag Lys 265 aag cgc ttc acc Lys Arg Phe Thr gac Asp 270 gtg gtc gcg caa Val Val Ala Gin ggt ggc gtg Gly Gly Val 275 ccg atg atc gtt ggg cag ggc gac atc att ccg gaa atg atc aaa tgg 1423 WO 01/00843 WO 0100843PCTIiBOO/00923 Pro Met Ile Val Gly Gin Gly Asp 280 285 ttc tcc gag aac ggc acc ctc cct 1027 Phe Ser Glu Asn Gly Thr Leu Pro 295 300 aag ggc agc gtg tgg gtg ttg agc 1 1075 Lys Gly Ser Val Trp Val Leu Ser 1 310 315 gct gat tac ctg gcg agt tcc ctg 1128 Ala Asp Tyr Leu Ala Ser Ser Leu 330 cca 1131 <210> 1038 <211> 336 <212> PRT <213> Corynebacterium glutamicum Ile Ile Pro Giu Met Ile Lys Trp 290 gag aag atc aag gcg aaa Glu Lys Ile Lys Ala Lys 305 gac ggt gtg ttc acc ggc Asp Gly Val Phe Thr Gly 320 325 aaa taggagcgcg tttaaggcct Lys :cg gtt Pro Val 335 <400> 1038 Met Ala Asn Lys Asn Asn Lys Pro Hius 1 Ser Thr Arg Arg Gly Gly Val Gly Ser 145 Asn Arg Asn Asp 70 Thr Leu Lys Pro Cys 150 Gly Pro Pro Trp Thr Gly Val Asn 135 Glu Leu Leu Ser Leu Ala Leu 105 Tyr Glu Leu Glu 10 Gin Ala Ile Ala Arg Ile Tyr Val1 Ser Arg 170 Val Gin Ala Glu Lys 75 Giu Gly Trp Asp Tyr 155 Thr Asp Ala Val Val Val Giu Thr 110 Gin Arg Asp Thr Gin Arg Leu Ile Asp Glu Tyr Val1 -Trp Thr Thr 175 Asp Pro Trp His Pro Thr Pro Leu Leu Giu 160 Arg Val Leu Ala Lys Ala Ala Lys Arg Phe 165 1424 WO 01/00843 Vai Leu Tyr Gly Asp Asp 195 Giu Met Leu 210 Ser Ala Val 225 Glu Leu Gly Trp Giu Thr Ala Gin Gly 275 Giu Met Ile 290 Lys Ile Lys 305 Gly Vai Phe Arg Lys Pro Asp Asp 245 Pro Val Trp Lys Gly 325 His Arg Met Arg 230 Vai Glu Pro Phe Lys 310 Ala Aia Pro Leu 215 Cys Ser Ala Met Ser 295 Giy Asp His Leu 200 Leu Gin Vai Cys Ile 280 Glu Ser Tyr Al a 185 Asp Pro Ala Asn Lys 265 Vai Asn Val Leu Giy Lys Lys Ala 235 Leu Arg Gin Thr Val1 315 Ser Arg Gly Pro 220 Leu Phe Phe Gly Leu 300 Leu Ser Thr 190 Arg Ala Leu Asp Asp 270 Ile Ile Phe Pro PCTIIBOO/00923 Trp Giy Gin Ala Ile Tyr Ala Asp 240 Asp Ala 255 Val Val Ile Pro Asp Giu His Asp 320 Val Lys 335 <210> 1039 <211> 757 <212> DNA <213> Corynebacteriun giutamicun <2 <221> CDS <222> (101)..(757) <223> FRXA0i024 <400> 1039 aatagatgga agtagttttt cattcactta tgtgcgcgtt gaactgtgtg caccacaacg cggaaggtga atcgcaccca aat aag cct cat gag gtg gac aaa gac caa gat Asn Lys Pro His Glu Val Asp Lys Asp Gin Asp 15 aac ggt cgc ctg caa cag atc ccg gcg cgt ccc Asn Giy Arg Leu Gin Gin Ile Pro Ala Arg Pro 30 cgc cca act ctt gca gca ggt gca gta ctg tgg Arg Pro Thr Leu Ala Ala Gly Ala Val Leu Trp 1425 tttaatctgg tttctaccaa atg gca aat aag aac 115 Met Ala Asn Lys Asn 1 tca gcc atg ctg atc 163 Ser Ala Met Leu Ile act gag gaa ttc acc 211 Thr Glu Glu Phe Thr cgc ggc gac atc acc 259 Arg Gly Asp Ile Thr WO 01/00843 WO 0100843PCTIBOOIOO923 aac ccg Asn Pro gac agc atc gag Asp Ser Ile Glu gct gtc atc cac Ala Val Ile His ccg cac tat gat Pro His Tyr Asp tgg tcc ctg gcc Trp Ser Leu Ala ggc aaa gtc gat Giy Lys Val Asp ggc gag tct att Gly Giu Ser Ile aca acc gcg gcc Thr Thr Ala Ala cgt Arg gaa atc ctt gaa Giu Ile Leu Glu act ggc tac gac Thr Gly Tyr Asp atc cgt Ile Arg 100 ctg ggc aag Leu Gly Lys aaa gtg gtc Lys Val Val 120 atc ggc aag gtt Ile Gly Lys Vai tac cct gtg ctc Tyr Pro Val Leu gac cga acc Asp Arg Thr 115 gag ttt gtc Giu Phe Val tac tac tgg act Tyr Tyr Trp Thr cag gtt ctt ggt Gin Val Leu Gly gga Gly 130 ccc aac Pro Asn 135 gat gaa gtt gat Asp Glu Val Asp atc cgt tgg ctg Ile Arg Trp Leu gtt gat gaa gca Vai Asp Giu Aia gag ttg Giu Leu ctc agc tac caa gta gat acc Leu Ser Tyr Gin Vai Asp Thr 155 gtt ctg gcc aag Val Leu Ala Lys gca aag cgt ttc Ala Lys Arg Phe cgc Arg 170 act cct tcc acc Thr Pro Ser Thr cgg gtg ctg tat Arg Vai Leu Tyr gtt cgc Vai Arg 180 cat gct cat His Ala His cgc cca ttg Arg Pro Leu 200 cat ggt cgc caa His Gly Arg Gin tgg ggt ggc gac Trp Gly Gly Asp gac aat aag Asp Asn Lys 195 ctc gta ccc Leu Val Pro gac aaa aag ggg Asp Lys Lys Gly cga caa gca gaa Arg Gin Ala Giu atg Met 210 atg ttg Met Leu 215 ttg ccc ttc aaa Leu Pro Phe Lys <210> 1040 <21i> 219 <212> PRT <213> Corynebacterium giutaxnicum <400> 1040 Met Ala Asn Lys Asn Asn Lys Pro His Giu 1 5 10 Val Asp Lys Asp Gin Asp Ser Ala Met Leu Ilie Asn Gly Arg Leu 25 Thr Giu Giu Phe Thr Arg Pro Thr Leu Gin Gin Ile Pro Vai Leu Trp. Ala Ala Gly 1426 WO 01/00843 WO 1/0843PCTIIBOO/00923.

Arg Gly Asp Ile Thr Asn Pro Asp Ser Arg Pro His Tyr Asp Asp Trp Ser Leu Gly Giu Ser Ile Pro Thr Thr Ala Ala Giy Tyr Asp Ile Arg Leu Gly Lys Leu 100 -105 Val Leu Asp Arg Thr Lys Val Val Tyr 115 120 Gly Gly Glu Phe Vai Pro Asn Asp Glu 130 135 Ser Vai Asp Glu Ala Cys Glu Leu Leu 145 150 Val Leu Ala Lys Ala Ala Lys Arg Phe 165 Val Leu Tyr Val Arg His Ala His Ala 180 185 Gly Asp Asp Asn Lys Arg Pro Leu Asp 195 200 Giu Met Leu Val Pro Met Leu Leu Pro 210 215 <210> 1041 <211> 257 <212> DNA <213> Corynebacterium glutainicum <22 0> <221> CDS <222> <223> FRXA01027 <400> 1041 acc gat ccc gag gcc tgc aag aag cgc Thr Asp Pro Glu Ala Cys Lys Lys Arg 1 5 ggt ggc gtg ccg atg atc gtt ggg cag Gly Gly Val Pro Met Ile Val Gly Gin 25 atc aaa tgg ttc tcc gag aac ggc acc Ile Lys Trp, Phe Ser Glu Asn Gly Thr 40 aag gcg aaa aag ggc agc gtg tgg gtg Lys Ala Lys Lys Gly Ser Vai Trp Vai 55 Ile Asp Giu Tyr Val1 Trp Thr Thr 175 Trp Gin ttc acc gac gtg Phe Thr Asp Vai 10 ggc gac atc att Gly Asp Ile Ile ctc cct atc gat Leu Pro Ile Asp ttg agc ttt cac Leu Ser Phe His gtc gcg caa Vai Ala Gin ccg gaa atg Pro Giu Met gag aag atc Giu Lys Ile gac ggt gtg Asp Gly Vai 1427 WO 01/00843 WO 0100843PCTLBOOIOO923 ttc acc ggc gct gat tac ctg gcg agt tcc ctg ccg gtt aaa Phe Thr Gly Ala Asp Tyr Leu Ala Ser Ser Leu Pro Val Lys 70 taggagcgcg tttaaggcct cca <210> 1042 <211> 78 <212> PRT <213> Corynebacterium giutamicum <400> 1042 Thr Asp Pro Glu Ala Cys Lys Lys Arg Phe Thr Asp Val Vai 1 5 10 Gly Giy Val Pro Met Ilie Val Giy Gin Gly Asp Ile Ile Pro 25 Ile Lys Trp Phe Ser Glu Asn Gly Thr Leu Pro Ile Asp Giu 40 Lys Ala Lys Lys Gly Ser Val Trp Val Leu Ser Phe His Asp 55 Phe Thr Gly Ala Asp Tyr Leu Ala Ser Ser Leu Pro Val Lys 70 <210> 1043 <211> 651 <212> DNA <213> Corynebacteriun giutanicun <22 0> <221> CDS <222> (101)..(628) <223> RXA01528 <400> 1043 cacccaaacc caaacctctc agtcgaataa gcagaagtct caggacaacc gggtcgtagg tctccaacca ggaggcgttc caacacgagg gtg aat cag Ala Gin Giu Met Lys Ile Gly Val gcaggggtaa gcg tgg cag Gin gtg Val ctg Leu cgt Arg act Thr gag Glu gcg Ala ccg Pro tct Ser gcg Ala ttg Leu aag Lys gat gag Asp Giu gtc aac VTal Asn att ggt Ile Gly ggc cat Gly His 1428 Val Asn Gin Ala Trp 1 tcc gca ggt ggt ctc Ser Ala Gly Gly Leu aac aat gag gtt gat Asn Asn Giu Val Asp ctt gat cgt cgt ggt Leu Asp Arg Arg Gly gag cct ggt gag gat Glu Pro Gly Giu ASP 115 163 211 259 307 WO 01/00843 aag gct gcg act gct gag..cgt gag gtg tgg Lys Ala Ala Thr Ala Giu Arg Giu Vai Trp 75 ggt gag gtg ttc act gag ttg ggt gtg att Gly Glu Val Phe Thr Giu Leu Gly Val Ile 95 gaa ggg aag cgg atc cat aag acg gtg cat Giu Gly Lys Arg Ile His Lys Thr Val His 105 110 gtt gat ggc gat ttg aat gat gag gat cca Val Asp Giy Asp Leu Asn Asp Giu Asp Pro 120 125 tgg att ccg gcg aat cag ttg att gag cat Trp Ile Pro Ala Asn Gin Leu Ile Giu His 135 140 cgg aag ttg gct agg cag gcg cat gat ttg Arg Lys Leu Ala Arg Gin Ala His Asp Leu 150 155 aag gaa aag gcg gag gga agg tcc acc cca Lys Giu Lys Ala*Giu Gly Arg Ser Thr Pro 170 175 aac <210> 1044.

<211> 176 <212> PRT <213> Corynebacterium giutanicum <400> 1044 Val Asn Gin Ala Trp Gin Gin Ser Arg Leu 1 5 10 Ser Ala Gly Gly Leu Vai Val Ser Gly Leu Asn Asn Glu Vai Asp Leu Ser Lys Ile Tyr 40 Leu Asp Arg Arg Gly Arg Leu Leu Trp Ser 55 Giu Pro Giy Glu Asp Lys Ala Ala Thr Ala 70 Glu Thr Gly Ile His Giy Glu Val Phe Thr 90 Tyr Trp Phe Vai Ser Giu Gly Lys Arg Ile 100 105 His Leu Leu Arg Tyr Val Asp Gly Asp Leu 1429 PCT/IBOO/00923 gag Glu 80 gat Asp cat His gaa Glu t tg Leu t tg Leu 160 agg Arg Val Al a Val Met Giu Glu His Asn gag acc ggc atc cac 355 Giu Thr Gly Ile His tat tgg ttc gtt tcg 403 Tyr Trp Phe Val Ser 100 cat ttg ttg cgt tat 451 His Leu Leu Arg Tyr 115 gtc act gag gtg gcg 499 Val Thr Glu Val Ala 130 gct ttt gcg gat gag 547 Ala Phe Ala Asp Glu 145 cct gag ttt gct ttg 595 Pro Glu Phe Ala Leu 165 tgattccgAa ccccaacccg 648 651 Thr Glu Ala Pro Arg Leu Lys Asp Ser Ala Leu Lys Glu Giy Thr Glu Giu Asn Gly His Trp Ile His Pro WO 01/00843 WO 0100843PCT/EBOO/00923 115 Val Thr Giu 130 Val Ala Trp, Pro Ala Asn Gin Leu Ile Giu His Leu 140 Ala Phe Ala Asp Glu Arg 145 150 Lys Leu Ala Arg Gin 155 Ala His Asp Leu Pro Glu Phe Ala Lys Glu Lys Aia Glu 170 Giy Arg Ser Thr Pro Arg 175 <210> <211> <212> <213> <220> <221> <222> <223> 1045 541

DNA

Co rynebactCeriuxn giutamicum

CDS

(101)..(541) RXA00072 <400> 1045 acggccagga cgatccagtg cacaggccag caccagcaaa gtccacatcg caagcattaa aagaatctct cgaaagacac aaaagaggtg agtcgcaaca atg agc ttt caa Met Ser Phe Gin 1 gtt aac gcc ctg Val Asn Ala Leu aat act ggt tcg Asn Thr Gly Ser aaa gat ccc gag Lys Asp Pro Glu atc tca Ile Ser ccc gaa gga Pro Giu Gly cat aac gag His Asn Giu cgc acg acc aca Arg Thr Thr Thr ttg tca cca gag Leu Ser Pro Glu gta gca aaa Val Ala Lys tat gac gcc Tyr Asp Ala gaa ctc gtc gaa Glu Leu Val Giu cat gct gct gcg His Ala Ala Ala agc gcg Ser Ala caa gag atc ctg Gin Giu Ile Leu tgg aca gcc gag Trp Thr Ala Giu cac His gcg ccg ggc gct Ala Pro Gly Ala at Ile gca gtg acc ttg agc atg gaa aac acc Ala Val Thr Leu Ser Met Glu Asn Thr ctg gcg gag ctg Leu Ala Glu Leu gcg cgg cac ctg Ala Arg His Leu gaa gct gat ttc Giu Ala Asp Phe ttt ttg gac acc Phe Leu Asp Thr ggt tac Gly Tyr 100 cac ttc aag His Phe Lys gag Giu 105 acc ctt gaa gtt gcc cgt cag gta gat Thr Leu Glu Val Ala Arg Gin Val Asp 110 gag cgc tat Giu Arg Tyr 115 tcc cag aag ctt gtc acc gcg ctg ccg atc ctc aag cgc acg gag cag 1430 WO 01/00843 PCTIIBOO/00923 Ser Gin Lys Leu Val Thr Ala Leu Pro Ile Leu Lys Arg Thr Giu Gin 120 125 130 gat tcc att tat ggt ctc aac ctg tac cgc agc aac cca gcg 541 Asp Ser Ile Tyr Gly Leu Asn Leu Tyr Arg Ser Asn Pro Ala 135 140 145 <210> 1046 <211> 147 <212> PRT <213> Corynebacterium glutamicun <400> 1046 Met Ser Phe Gin Leu Val Asn Ala Leu Lys Asn Thr Gly Ser Val Lys 1 5 10 Asp Pro Glu Ile Ser Pro Giu Gly Pro Arg Thr Thr Thr Pro Leu Ser 25 Pro Giu Val Ala Lys His Asn Glu Glu Leu Val Glu Lys His Ala Ala 40 Ala Leu Tyr Asp Ala Ser Ala Gin Glu Ile Leu Glu Trp Thr Ala Glu 55 His Ala Pro Gly Ala Ile Ala Val Thr Leu Ser Met Giu Asn Thr Val 70 75 Leu Ala Glu Leu Ala Ala Arg His Leu Pro Glu Ala Asp-Phe Leu Phe 90 Leu Asp Thr Gly Tyr His Phe Lys Glu Thr Leu Giu Val Ala Arg Gin 100 105 110 Val Asp Giu Arg Tyr Ser Gin Lys Leu Val Thr Ala Leu Pro Ile Leu 115 120 125 Lys Arg Thr Glu Gin Asp Ser Ile Tyr Gly Leu Asn Leu Tyr Arg Ser 130 135 140 Asn Pro Ala 145 <210> 1047 <211> 1002 <212> DNA <213> Corynebacteriui giutainicum <22 0> <221> CDS <222> (101)..(979) <223> RXA01878 <400> 1047 ggttcaggct gcacaaggtt ggggagcatg gcctgcttgc accgcaagct tgggcatccg atagtagaaa tctggCatcc aataggtaga ttgggatgct atg gaa gaa ccc tca 115 Met Giu Giu Pro Ser 1 1431 WO 01/00843 WO 0100843PCT/JIBOO/00923 ggt gca cag ctg Gly Ala Gin Leu ggc ccg gta gaa Gly Pro Val Glu cgt gcg ctg gca Arg Ala Leu Ala gaa aag Giu Lys ctc gac gtc Leu Asp Val ccc aac acg Pro Asn Thr aca Thr cca act aag aag Pro Thr Lys Lys ggg cag aac ttt Gly Gin Asn Phe gtt cac gat Val His Asp acc cca aac Thr Pro Asn gtg cgt cgc att Val Arg Arg Ile gct gcg gca gag Ala Ala Ala Giu gac cac Asp His gtg gtg gaa gtt Val Val Giu Val cct ggt ctg ggc Pro Gly Leu Gly tct Ser ctg acc ctt gcc Leu Thr Leu Ala gtg gaa tct gct Val Giu Ser Ala tca gta act gcg Ser Val Thr Ala gaa att gat ccc Giu Ile Asp Pro ttg gct gcg gaa Leu Ala Ala Giu ccg gag act ttt Pro Giu Thr Phe tgg cgc gcg ccg Trp Arg Ala Pro gcc ctt Ala Leu 100* gct cac aag Ala His Lys tcc gat atg Ser Asp Met 120 ttg Leu 105 agc atc gtg ctg Ser Ile Val Leu gac gcc ctg aag Asp Ala Leu Lys gtt caa caa Val Gin Gin 115 ttg ccg tac Leu Pro Tyr gct gtt caa ccc Ala Val Gin Pro gcc ttg gtg gct Ala Leu Val Ala aac Asn 130 aac gtc Asn Val 135 tct gtc cct gtc ttg ttg cac atg atg Ser Val Pro Val Leu Leu His Met Met 140 gag Giu 145 gag ttt ccc acc Giu Phe Pro Thr aac aag gtg ctt Asn Lys Val Leu gtc Val 155 atg gtg cag gca Met Val Gin Ala gtt gct gat cgt Val Ala Asp Arg gct gcg gat cca Ala Ala Asp Pro gga Gly 170 tcg aag att tat Ser Lys Ile Tyr gtg cct agc gtg Val Pro Ser Val aag gcg Lys Ala 180 tcc ttc tac Ser Phe Tyr ttt tgg cca Phe Trp Pro 200 cca gtt act cgc Pro Val Thr Arg ggg tcg att ggt Gly Ser Ile Gly aag aat gtc Lys Asn Val 195 atc gtg cgc Ile Val Arg 691 739 gct cca aag atc Ala Pro Lys Ile tcc ggt ttg gtg Ser Gly Leu Val aag Lys 210 gaa gac Glu Asp 215 acc gcg tgg aag Thr Ala Trp, Lys cag Gin 220 gac gat gag acg Asp Asp Giu Thr aag aag gtg tgg Lys Lys Val Trp atc att gat gct Ilie Ile Asp Ala gc t Ala 235 ttc ttg cag cgC Phe Leu Gin Arg aaa acc cta aga Lys Thr Leu Arg gc t Ala 245 1432 WO 01/00843 PCT/IB00/00923 gcg ctt tct gga cac tac ggt tct ggc cag gca get gag gaa get ttg 883 Ala Leu Ser Gly His Tyr Gly Ser Gly Gin Ala Ala Glu Glu Ala Leu 250 255 260 cgg gcc get gat att gat cca acg ctt cgt ggc gaa aag ctt gat gtc 931 Arg Ala Ala Asp Ile Asp Pro Thr Leu Arg Gly Glu Lys Leu Asp Val 265 270 275 act gac tat gtg cgc cta get ggg gtg ttg cag caa aag gat gag aag 979 Thr Asp Tyr Val Arg Leu Ala Gly Val Leu Gin Gin Lys Asp Glu Lys 280 285 290 tgaaaattac cgctaaggcg tgg 1002 <210> 1048 <211> 293 <212> PRT <213> Corynebacterium glutamicum <400> 1048 Met Glu Glu Pro Ser Gly Ala Gin Leu 1 Ala Asn Glu Ser Glu Arg Leu Ala Glu 145 Val Pro Ile Ala Val Thr Thr Asp Pro Val 115 Leu Phe Asp Val Lys 195 Val Thr 40 Val Glu Ala Lys Met 120 Ser Lys Asp Tyr Pro 200 Thr Val Val Ser Glu Leu 105 Ala Val Val Pro Gly 185 Ala Leu 10 Pro Arg Glu Ala Leu Ser Val Pro Leu Gly 170 Pro Pro Gly Thr Arg Val Ala 75 Pro Ile Gin Val Val 155 Ser Val Lys Pro Lys Ile Gly Ser Glu Val Pro Leu 140 Met Lys Thr Ile Val Glu Lys Leu Val Ala Pro Gly Val Thr Thr Phe Leu Lys 110 Thr Ala 125 Leu His Val Gin Ile Tyr Arg Ala 190 Glu Ser 205 Ile Gly Ala Leu Ala Gin Asp Leu Met Ala Gly 175 Gly Gly Arg Gin Ala Gly Val Trp Ala Val Met Glu 160 Val Ser Leu 1433 WO 01/00843 Val Lys Ile Val Arg Giu Asp Thr Ala Trp, Lys 210 215 Arg Lys Lys Val Trp Pro Ile Ile Asp Ala Ala 225 230 -235 Lys Thr Leu Arg Ala Ala Leu Ser Giy His Tyr 245 250 Ala Glu Glu Ala Leu Arg Ala Ala Asp Ile Asp 260 .265 Giu Lys Leu Asp Val Thr Asp Tyr Val Arg Leu 275 280 Gin Lys Asp Giu Lys 290 <210> 1049 <211> 1545 <212> DNA <213> Corynebacteriun giutainicum <220> <221> CDS <222> (1531) <223> RXN02281 <400> 1049 aagatcaaca acgccgccga ggtcaacttg gaaattatca cctataaaag cacagttttg aatccacagg gcatcagggc Gin 220 Phe Gly Pro Ala Asp Leu Ser Thr Gly 285 PCT/1BO0100923 Asp Giu Thr Gin Arg Arg 240 Gly Gin Ala 255 Leu Arg Gly 270 Val Leu Gin cgtgaggatt cttgcatacg gtg Val gac Asp gag Glu gac Asp gtg Val att Ile ccc gca Ala acc Thr gca Ala tgg Trp ggg Gly c tc Leu tac gtg Val 1 gca Ala aaa Lys tac Tyr acc Thr ttg Leu cgt Arg atc atc Ile gaa Giu ccc Pro gaa Glu tcc Ser ctc Leu cgc cag aaa gat agt Gin Lys Asp Ser gcg Al a acc Thr aaa Lys ccc Pro aaa Lys ac c Thr atc 115 163* 211 259 307 355 403 451 1434 WO 01/00843 Tyr Pro Cys gag tac att Glu Tyr Ile 120 gat gtt gca Asp Vai Ala 135 gca att atc Ala Ile Ile 150 ctt ttc cac Leu Phe His gag cac tac Glu His Tyr ttc acc aac Phe Thr Asn 200 tcc gaa ctc Ser Giu Leu 215 aac-ggg cag Asn Gly Gin 230 acc ctt gat Thr Leu Asp acc gaa gcc Thr Giu Aia tcc aat gca Ser Asn Ala 280 gcc tgg gtg 1027 Ala Trp, Val 295 atc ggc gac 1075 Ile Giy Asp 310 ctg aat acc 1123 Leu Asn Thr Ile ggC G ly atc Ile ggC Gly ggg Gly 170 ggc Gly T[yr gca Ala atc Ile gac Asp 155 ccg Pro atc Ile gcc atg cac Ala Met His tcg Ser att Ile act Thr 250 gac Asp act Thr atc Ile atc Ile atc Ile 330 gaa Giu gac Asp 235 agc Ser ggt Gly atc Ile Gly ctt Leu 315 c ca Pro gac Asp 220 cga Arg cgt Arg at t Ile ac c Thr cac His 300 ggc Gly ctt Leu Asp gaa Giu ctg Leu aac Asn 160 atc Ile gtg Val t tc Phe gtg Val1 acc.

Thr 240 cc t Pro aac Asn gct Ala atg Met cag Gin 320 atc Ile Ile gct Ala gat Asp 145 ggc Gly gcc Ala cag Gin gtg Vai ggt Giy 225 agc Ser cgt Arg atc Ile gtg Vai gac Asp 305 cgc Arg ccg Pro Arg cgc Arg 130 gaa Giu ccc Pro tgc Cys aag Lys cat His 210 cta Leu ctc Leu.

tac Tyr ggt Gly ctc Leu 290 gcc Al a gaa Giu gca Ala Ile 115 cgt Arg gtc Val1 gaa Giu gcc Al a tac Tyr 195 ttt Phe tcc Ser ggt Giy gat Asp gtg Val1 275 cgc Arg cgc Arg gac Asp ata Ile PCTIBOO/00923 ro Pro ct ggt 499 la Gly ac gac 547 is Asp tc ccg 595 eu Pro 165 gc atc 643 rg Ile tt ctg 6-91 le Leu cc atg 739 la Met t tg Leu acg Thr ctc Leu 260 ggc Gly cca Pro atg Met cac His cca Pro 340 cca Pro gaa Giu 245 atc Ile cca Pro gaa Giu cgc Arg att Ile 325 gaa Giu 1435 WO 01/00843 WO 0100843PCTIiBOO/00923 atc caa 1171 Ile Gin aac agc 1219 Asn Ser tgg gaa 1267 Trp Giu 375 acc gcc 1315 Thr Aia 390 tat cga 1363 Tyr Arg cca cta 1411 Pro Leu ttc aac 1459 Phe Asn tac ctg 1507 Tyr Leu 455 acc ttg 1545 Thr Leu 470 aaa gct Lys Ala 345 ctc atg Leu Met cta gaa gcc agc gtc Leu Giu Ala Ser Val 350 cgc acc ggt acg gtc Arg Thr Gly Thr Val 365 acc cca gaa aac ctc Thr Pro Giu Asn Leu gaa atc tac gga tcc gac Giu Ile Tyr Gly Ser Asp 355 tcc acc gac gac cga aac Ser Thr Asp Asp Arg Asn 370 aac tgg ctc aaa gga tcc Asn Trp Leu Lys Gly Ser cac His tgg Trp, 380 385 gca gct gtt gac Ala Ala Val Asp 395 ttc cgc att cca Phe Arg Ile Pro 410 atg gaa tct tcc Met Glu Ser Ser tac ggc acc ctg Tyr Gly Thr Leu 415 ac c Thr 400 ttg Leu gcc acc aac gga Ala Thr Asn Gly 405 cac gca His Ala 425 tcc aag Ser Lys 440 gca gta Ala Val gtg ccg aaa ctt tcc Val Pro Lys Leu Ser 430 gaa gcc cac gtc atg Glu Ala His Val Met 445 gat cct gaa cgg ttg Asp Pro Glu Arg Leu gcg Ala tgt Cys ctg agc gtc tct gac ctg Leu Ser Val Ser Asp Leu 420 caa gcg cag gcg ttt tac Gin Ala Gin Ala Phe Tyr 435 gct gtt cgt gca atg gaa Ala Val Arg Ala Met Glu 450 cgt acc cgt aaa ctg cgc agg Arg Thr Arg Lys Leu Arg Arg 465 460 ggt gag gtg ccg ttt cgc Gly Glu Val Pro Phe Arg 475 taaagtctcg gaga <210> 1050 <211> 477 <212> PRT <213> Corynebacterium giutamicun <400> 1050 Val Gin Lys Asp Ser Val Val Arg Met Giu Ala Thr Thr Ile Asp Asp 1 5 10 Ala Ile Ala Lys Leu Ile Asp Ile Tyr Asp Thr Ser'Thr Lys Leu Ala 25 Lys Glu Thr Leu Asn Asn Glu Asp Tyr Ala Ala Tyr Ala Asp Val Val 40 1436 WO 01/00843 WO 0100843PCTIIBOO/00923 Tyr Thr Leu Arg Ile Ala Asp 145 Gly Ala Gin Val Gly 225 Ser Arg Ile Val Asp 305 Arg Pro Ile Lys Pro Lys Thr Ile 115 Arg Vai Giu Ala Tyr 195 Phe Ser Gly Asp Val 275 Arg Arg Asp Ile Gly 355 Val Tyr 70 Val1 Tyr Giu Asp Ala 150 Leu Giu Phe Ser Asn 230 Thr Thr Ser Ala Ile 310 Leu Ile Asn Asp Val1 Ile Pro Tyr Val 135 Ile Phe His Thr Giu 215 Giy Leu Giu Asn Trp 295 Giy Asn Gin Ser Val Asp Glu Cys Ile 120 Ala Ile His Tyr Asn 200 Leu Gin Asp Ala Ala 280 Val Asp Thr Lys Leu 360, Leu Arg Arg Lys 105 Arg Asp Asp Phe Thr 185 Tyr Thr Val Leu Gly 265 Lys Met Leu Arg Ala 345 Met Giu Ala Tyr Ile Gly Ile Gly Gly 170 Gly Ala Ser Ile Thr 250 Asp Thr Ile Ile Ile 330 Leu Arg Trp Gly 75 Leu Tyr Al a Ile Asp 155 Pro Ile Met Glu Asp 235 Ser Gly Ile Gly Leu 315 Pro Giu Thr Lys Arg Arg Vai Pro Pro 140 Trp Gin Asn His Asp 220 Arg Arg Ile Thr His 300 Gly Leu Ala Gly Pro Tyr Glu Ser Ser 125 Arg His Arg Val Thr 205 Ser Giu Phe Thr Asp 285 Cys Asn Gly S er Thr 365 Ile S er Gin Giu 110 Ala Pro Ala Phe Giu 190 Thr Arg Thr Gin Ile 270 Cys Ala Ala Asn Val 350 Val1 Asp Ala Leu Ser Thr Thr Phe Asp 175 His Giu Tyr Ala Met 255 Ile Leu Gly Tyr Pro 335 Asp Leu Arg Thr Giu Asp Giu Leu Asn 160 Ile Val1 Phe Val1 Thr 240 Pro Asn Ala Met Gin 320 Ile Giu Ser 1437 WO 01/00843 Thr Asp Asp Arg Asn Trp Glu Trp His Thr Pro 370 375 Trp Leu Lys Gly Ser Thr Ala Ala Ala Val Asp 385 390 395 Leu Ala Thr Asn Gly Tyr Arg Phe Arg Ile Pro 405 410 Ser Val Ser Asp Leu Pro Leu His Ala Val Pro 420 425 Ala Gin Ala Phe Tyr Phe Asn Ser Lys Glu Ala 435 440 Val Arg Ala Met Glu Tyr Leu Ala Val Asp Pro 450 455 Arg Lys Leu Arg Arg Thr Leu Giy Glu Val Pro 465 470 475 <210> 1051 <211> 1191 <212> DNA <213> Corynebacteriun glutamicun <22 0> <221> CDS <222> (101)..(1168) <223> FRXA02281 <400> 1051 aagatcaaca acgccgccga ggtcaacttg gaaattatca cctataaaag cacagttttg aatccacagg gcatcagggc Glu 380 Met Tyr Lys His Glu 460 Phe PCTILBOO/00923 Trp Asn Ser Thr 400 Leu Leu 415 Ala Gin Cys Ala Arg Thr gtg Val gac Asp gag Glu gac Asp gtg Val att Ile cgc Arg atc Ile gac Asp gtg Val gat Asp gag Glu atg Met tac Tyr tac Tyr ctg Leu cga Arg cgt Arg acg atc gat Thr Ile Asp acc aaa ctg Thr Lys Leu 30 gcc gat gtt Ala Asp Val 45 ccc atc gac Pro Ile Asp tac tcc gcc Tyr Ser Ala gaa caa ctc Glu Gin Leu 1438 cgtgaggatt cttgcatacg gtg cag aaa gat agt Val Gin Lys Asp Ser 1 gca atc gcg aag ctc Ala Ile Ala Lys Leu aaa gaa acc ctc aac Lys Giu Thr Leu Asn tac ccc aaa ctc acc Tyr Pro Lys Leu Thr acc gaa ccc ttc ggc Thr Giu Pro Phe Gly ttg tcc aaa cca cgc Leu Ser Lys Pro Arg cgt ctc acc agt aat Arg*Leu Thr Ser Asn WO 01/00843 WO 0100843PCT/IBOO/00923 tat ccc tgc Tyr Pro Cys gag tac att Glu Tyr Ile 120 aag Lys 105 att tac gta tct Ile Tyr Val Ser tca gat atc cgc Ser Asp Ile Arg atc cca ccg Ile Pro Pro 115 cgt gct ggt Arg Ala Gly cgc ggc gca cct Arg Gly Ala Pro gct acc gaa gct Ala Thr Giu Ala cgc Arg 130 gat gtt Asp Val 135 gca gat atc atc cca cgc ccc acc ctg Ala Asp Ilie Ile Pro Arg Pro Thr Leu gat Asp 145 gaa gtc cac gac Giu Val His Asp gca Ala 150 att atc gac ggc gac tgg cac gcc ttc Ile Ilie Asp Gly Asp Trp, His Ala Phe 155 ggc ccc gaa ctc Gly Pro Giu Leu ctt ttc cac ttc Leu Phe His Phe ggg Gly 170 ccg caa cgc ttc Pro Gin Arg Phe atc gcc tgc gcc Ile Ala Cys Ala cgc atc Arg Ile 180 gag cac tac Giu His Tyr ttc acc aac Phe Thr Asn 200 acc Thr 185 ggc atc aac gtg Gly Ilie Asn Val cac gtg cag aag His Val Gin Lys tac att ctg Tyr Ile Leu 195 ttt gcc atg Phe Ala Met tac gcc atg cac Tyr Ala Met His acc gag ttc gtg Thr Giu Phe Val cat His 210 tcc gaa Ser Giu 215 ctc acc tcg gaa Leu Thr Ser Giu tcc cgc tac gtg Ser Arg Tyr Val ggt Gly 225 ct4 tcc ttg cca Leu Ser Leu Pro ggg cag gta att Gly Gin Vai Ile gac Asp 235 cga gag acc gcc Arg Giu Thr Ala agc ctc ggt acg Ser Leu Giy Thr gaa Giu 245 acc ctt gat ctg Thr Leu Asp Leu act Thr 250 agc cgt ttc caa Ser Arg Phe Gin cct cgt tac gat Pro Arg Tyr Asp ctc atc Leu Ile 260 acc gaa gcc Thr Giu Aia tcc aat gca Ser Asn Ala 280 ggt Gly 265 gac ggt att acc Asp Gly Ilie Thr atc aac atc ggt Ile Asn Ile Gly gtg ggc cca Val Gly Pro 275 cgc cca gaa Arg Pro Giu aaa act atc acc Lys Thr Ilie Thr tgc ctt gct gtg Cys Leu Aia Val ctc Leu 290 gcc tgg 1027 Ala Trp 295 atc ggc 1075 Ile Gly 310 gtg atg atc 99c cac tgt gct ggc atg gac gcc cgc atg cgc Val Met Ile Gly Cys Aia Gly Met Ala Arg Met Arg gac ctc atc ctt ggc aac gcc tac cag cgc gaa gac cac att Asp Leu Ile Leu 315 Gly Asn Ala Tyr Arg Giu Asp His Ile 325 1439 WO 01/00843 WO 0100843PCTIIBOO/00923 ctg aat acc cgc atc cca ctt ggt aat ccg atc ccg gca ata cca gaa 1123 Leu Asn Thr Arg Ile Pro Leu Gly Asn Pro Ile Pro Ala Ile Pro Glu 330 335 340 atc caa aaa gct cta gaa gcc agc gtc gac gaa atc tac gga tcc 1168 Ile Gin Lys Ala Leu Glu Ala Ser Val Asp Giu Ile Tyr Gly Ser 345 350 355 tagtattcta tagtgtcacc taa 1191 <210> 1052 <211> 356 <212> PRT <213> Corynebacteriun giutamicum <400> 1052 Val Gin Lys Asp Ser Val Val Arg Met Giu Ala Thr Thr Ile Asp Asp 1 5. 10 Ala Ile Ala Lys Leu Ile Asp Ile Tyr Asp Thr Ser Thr Lys Leu Ala 25 Lys Glu Thr Leu Asn Asn Glu Asp Tyr Ala Ala Tyr Ala Asp Val Val 40 Tyr Pro Lys Leu Thr Val Asp Val Leu Glu Trp Lys Pro Ile Asp Arg 55 Thr Giu Pro Phe Gly Tyr Val Asp Arg Ala Gly Arg Tyr Ser Ala Thr 70 75 Leu Ser Lys Pro Arg Val Ile Giu Arg Tyr Leu Arg Glu Gin Leu Glu 90 Arg Leu Thr Ser Asn Tyr Pro Cys Lys Ile Tyr Val Ser Glu Ser Asp 100 105 110 Ile Arg Ile Pro Pro Glu '[yr Ile Arg Gly Ala Pro Ser Ala Thr Glu 115 120 125 Ala Arg Arg Ala Gly Asp Val Ala Asp Ile Ile Pro Arg Pro Thr Leu 130 135 140 Asp Glu Val His Asp Ala Ile Ile Asp Gly Asp Trp His Ala Phe Asn 145 150 155 160 Gly Pro Glu Leu Pro Leu Phe His Phe Gly Pro Gin Arg Phe Asp Ile 165 170 175 Ala Cys Ala Arg Ile Glu His Tyr Thr Gly Ile Asn Val Glu His Val 180 185 190 Gin Lys Tyr Ile Leu Phe Thr Asn Tyr Ala Met His Thr Thr Glu Phe 195 200 205 Val His Phe Ala met Ser Glu Leu Thr Ser Glu Asp Ser Arg Tyr Val 210 215 220 1440 WO 01/00843 WO 0100843PCTIIBOO/00923 Giy Leu Ser Leu Pro Asn Gly Gin Val Ile Asp 225 230 235 Ser Leu Giy Thr Giu Thr Leu Asp Leu Thr Ser 245 250 Arg Tyr Asp Leu Ilie Thr Giu Ala Gly Asp Gly 260 265 Ile Gly Val Gly Pro Ser Asn Aia Lys Thr Ile 275 280 Vai Leu Arg Pro Giu Ala Trp Vai Met Ile Giy 290 295 Asp Aia Arg Met Arg Ile Gly Asp Leu Ile Leu 305 310 315 Arg Giu Asp His Ile Leu Asn Thr Arg Ile Pro 325 330 Pro Ala Ilie Pro Giu Ilie Gin Lys Ala Leu Giu 340 345 Ile Tyr Giy Ser 355 <210> 1053 <211> 1146 <212> DNA <213> Corynebacterium giutamicuni <220> <221> CDS <222> (101)..(1123) <223> RXN01240 <400> 1053 ttgttctcac atctacccgt atctttgttg gttttagtac ctgcacagga aagttagcgg cgctactatg aacgatcgat Giu Phe Thr Asp 285 Cys Asn Giy Ser Thr Gin Ile 270 Cys Ala Ala Asn Vai 350 ctc tcc caa Leu Ser Gin atg Met ggt Gly agc Ser ggc Gly gaa Giu ttc Phe tac Tyr c ta Leu gtc Val1 aag Lys cac gaa His Giu 15 gca att Ala Ilie acc aca Thr Thr cgt cgc Arg Arg ttc Phe gaa Giu cgc Arg aac Asn ccgatagtga gttttgatcc atg tct gac aac act Met Ser Asp Asn Thr 1 cga cgt gcc cat ccc Arg Arg Ala His Pro gaa tta ctt acg gac Giu Leu Leu Thr Asp atc aaa gaa tgg tca Ile Lys Giu Tip, Ser gat ggc tcg ttg atc Asp Giy Ser Leu Ile 115 163 211 259 307 gcc aag gct Ala Lys Ala 1441 WO 01/00843 WO 0100843PCT/1BOOI00923 tac Tyr cct gat ccg cgc Pro Asp Pro Arg gac atc cac gac Asp Ile His Asp atc ggt gtt cgg Ile Gly Val Arg atc Ile acc acg tac cac Thr Thr Tyr His acg gaa ata ccc Thr Giu Ile Pro gcc cta aaa gtg Ala Leu Lys Val ctc caa Leu Gin 100 355 403 451 gac tcc ttc Asp Ser Phe atc tca ggc Ile Ser Gly 120 gtc cac aaa tcc Val His Lys Ser gac aaa gcc gcc gaa act cgc Asp Lys Aia Ala Giu Thr Arg 115 ggc ttt ggt tac Gly Phe Gly Tyr ggc Gly 125 tcc cac cac ctg Ser His His Leu ctg gaa gtc Leu Giu Val gat gac Asp Asp 135 acc tcc gat gac Thr Ser Asp Asp cag gac tac aaa Gin Asp Tyr Lys ctc gtc ttt gaa Leu Val Phe Giu gtt Val1 150 cag gtg cgc acc Gin Val Arg Thr ctg caa cac gcc Leu Gin His Ala gca gag ttc gaa Ala Giu Phe Giu gat atc cgc tat Asp Ile Arg Tyr cgc gcc gat gtg tcc aac cca gaa gac Arg Ala Asp Val Ser Asn Pro Giu Asp 175 ccc agc Phe Ser 180 gca gaa gta Ala Giu Val gcg gac caa Ala Asp Gin 200 cgc atg ttc acc Arg Met Phe Thr cc Leu 190 gctgcc gga ctc Ala Ala Giy Leu atc gaa tta Ile Giu Leu 195 acc agc cga Thr Ser Arg caa ttc gac caa Gin Phe Asp Gin gcc gca ctc aaa Ala Ala Leu Lys gtt gct gat gaa ccc gtc Val Ala Asp Giu Ser Val 215 ctc acc gca gag aca ctt ccc ggc gtt Leu Thr Ala Giu Thr Leu Pro Gly Val 225 ctt Leu 230 gcc acg ctc att Ala Met Leu Ile aac cgc ccc ccc Asn Arg Phe Pro cgc Arg 240 cca cgc tcc aca Pro Arg Ser Thr tac cgc ctc ctc Tyr Arg Phe Leu gaa Giu 250 gac atc ctg gtg Asp Ile Leu Val gcc Ala 255 aac tcc act acc Asn Ser Ile Thr tct gtg Ser Val 260 gtg cag ctg cgc gag ccg ctc aac Val Gin Leu Arg Glu Leu Leu Asn 265 ccc Pro 270 acc gac act gaa Thr Asp Ile Giu gtg-ctg ctg Vai Leu Leu 275 atc atc gac Ile Ile Asp aaa gta atg Lys Val Met 280 aac tac cgc ctc Asn Tyr Arg Phe cca ggc cag atc Pro Gly Gin Ile cgc Arg 290 gat tta cta ccc aaa cga ttt ggc caa cca cac atc gat gcc acc gtc 1027 Asp Leu 295 Leu Leu Lys Arg Gly Gin Ser His Ile 305 Asp Ala Thr Val 1442 WO 01/00843 WO 0100843PCT/iBOO/00923 gcc acc gac tcc caa cca ctc aac gcc aaa cgc cac agg Cag cta aaa 1075 Ala Thr Asp Ser Gin Pro Leu Asn Ala Lys Arg His Arg Gin Leu Lys 310 315 320 325 cgc aag cta gag ctc atg acc caa gct cat ctt gtg gaa cca cca aac 1123 Arg Lys Leu Giu. Leu Met Thr Gin Ala His Leu Vai Giu Pro Pro Asn 330 335 340 taaagttccc ccaaagttag ccc 1146 <210> 1054 <211> 341 <212> PRT <213> Corynebacterium giutamicun <400> 1054 Met Ser Asp Asn Thr Leu Ser Gin Phe 1 Arg Giu Ile Asp Ile Leu Ala Leu Gly 145 Ala Pro Gly Ala Leu Giu Ser Val Vai Giu 115 Leu Val1 Phe Asp Ile 195 His Thr Trp Leu Arg Leu 100 Thr Glu Phe Giu Phe 180 Glu 5 Pro Asp Ser Ile Ile Gin Arg Val Giu His 165 Ser Leu Va i 25 Thr Ala Pro His Ile 105 Gly Ser Arg Tyr Asp 185 Gin Tyr Leu Val Lys Ile Ile Ser Gly 125 Gin Gin Asp Thr Ile 205 Leu His Ala Thr Arg His Pro Val1 110 Ser Asp His Val Leu 190 Ala Thr Giu Ile Thr Arg Asp Val1 Asp His Tyr Aia Ser 175 Ala Ala Ala Lys Giu Thr Ser Arg Val Ala Asp Giu Ser 1443 WO 01/00843 210 Thr Leu Pro 225 Pro Arg Ser Ser Ilie Thr Ilie Giu Val 275 Ilie Arg Ile 290 Ile Asp Ala 305 His Arg Gin Val Glu Pro PCTIIBOO/00923 Gly Thr Ser 260 Leu Ile Thr Leu Pro 340 Giy 235 Asp Leu Arg Arg Pro 315 Met <210> 1055 <2i1> 1234 <2i2> DNA <2i3> Corynebacterium giutamicum <2 <221> CDS <222> (101)..(1234) <223> RXN02008 <400> 1055 tactggtcta tctatcaaaa gatgatcgtt cgcggtcgtg cttgttggca tccgcatcct ggtagacaac gtgaacaact ggt Gly tat Tyr gtg Val1 gac Asp aaa cac His gcc Ala cc t Pro tac Tyr aaa gct ctg Ala Leu gtc tac Val Tyr 30 ctg gaa Leu Giu ggc att Gly Ile ggc gag 1444 attttgacga tatttttgat gtg tac gcc gcc atc Val Tyr Ala Ala Ile 1 ggc cga ttc aaa gac Gly Arg Phe Lys Asp tcc ctg cac acc acc Ser Leu His Thr Thr cag gca cgt acc cac Gin Ala Arg Thr His gcg cac tgg cga tac Ala His Trp Arg Tyr gcc gaa gtg gat caa WO 01/00843 Lys Glu Tb atg gcg tg Met Ala Tr ccc aac ga Pro Asn Gi atc ttc gt Ile Phe Va 12 tcc acc cc Ser Thr Pr 135 cgc tgc at Arg Cys Ii 150 aaa ctc aa Lys Leu Ly aac gct gg Asn Ala Gi gca aag gc Ala Lys Al tac cta ga Tyr Leu Gi 215 ggc ctg cc Gly Leu Pr 230* gca aca ga Ala Thr Gi ggc tcc gg Gly Ser GI atc ttt gg Ile Phe Gi 28 cca ttc ag 1027 Pro Phe Se 295 Gly cgc Arg ctg Leu aca Thr gac Asp gcc Ala ggc Gly 170 agt Ser att Ile gga Gly cac His cac His 250 gta Val gaa Glu c tg Leu Ser 75 caa Gin gac Asp ccc Pro t tc Phe aaa Lys 155 gat Asp agg Arg cgc Arg cgc Arg cgc Arg 235 tac Tyr tc t Ser gaa Giu gtc Val1 His ctt Leu agc Ser aaa Lys gcc Ala 140 atc Ile cgt Arg gga Gly cag Gin ga t Asp 220 t tg Leu cca Pro gcg Ala gat Asp aac Asn 300 Ser ctg Leu c tg Leu ggt Giy 125 tac Tyr aac Asn gtt Val tgg Trp tgg Trp, 205 gcg Ala ttc Phe gat Asp caa Gin gcc Ala 285 tcc Ser Ala aaa Lys ctg Leu aac Asn acc Thr 145 gtc Val acc Thr gtt Val gaa Glu gtt Val1 225 tcc Ser ctc Leu aac Asn t tg Leu acg Thr 305 Val gca Ala tcc Ser 115 ccg Pro gtg Val ttg Leu aag Lys tca Ser 195 cgc Arg cag Gin aag Lys aca Thr ctc Leu 275 gca Ala agc Ser PCTIBOOIOO923 Asp Gin gcc gac 403 Ala Asp 100 aag cag 451 Lys Gin gtg aac 499 Val Asn ggg cac 547 Gly His gaa acg 595 Giu Thr 165 gac caa 643 Asp Gin 180 cct cgt 691 Pro Arg gaa gaa 739 Glu Giu cgt ggc 787 Arg Gly acg gtg 835 Thr Val 245 gcc atc 883 Ala Ile 260 atg gct 931 Met Ala cgc acc 979 Arg Thr agc acc Ser Thr 1445 WO 01/00843 ggc atc ctg gtc gaa ggc agc cca gat 1075 Gly Ile Leu Val Glu Gly Ser Pro Asp 310 315 tgc tgt atg cca gtg cca gga gat gaa 1123 Cys Cys Met Pro Val Pro Gly Asp Glu 330 ggt ggc ggt gtc tcc gta cac cga aca 1171 Gly Gly Gly Val Ser Val His Arg Thr 345 350 ctc aaa gaa gag cca gaa cgc att gtc 1219 Leu Lys Glu Glu Pro Giu Arg Ile Val 360 365 ggt caa ggt tca gta 1234 Gly Gin Gly Ser Val 375 <210> 1056 <211> 378 <212> PRT <213> Corynebacterium giutamicum <400> 1056 Val Tyr Ala Ala Ile Gly Val Val His gtc Val atc Ile 335 gac Asp tcc Ser Ser 10 Pro Gly Asn Gly Arg 90 Leu Thr Asp Ala gc t Ala gga Gly acg Thr tcc Ser Phe Phe Lys Giu His Leu Ser Lys Ala 140 Ile aag Lys ttc Phe aat Asn tgg Trp 370 Asn Gly Pro Phe Ser Leu Leu Gly 125 Tyr Asn ctc Leu gtc Val gtg Val 355 gc t Al a Ala Val Leu Gly Gly Asp Arg 110 Asp Ala Gly PCTIiBOO/00923 gct aaa Ala Lys 325 acc cgt Thr Arg 340 gaa aag Giu Lys tcg gaa Ser Glu Leu Pro Tyr Gin Glu Val Ile Ala Glu Gin Trp, Gin Tyr Asp Val Val Val His Lys Leu 1 Gly Ser Gin Ala Al a Lys Leu Asn Thr 145 Lys Thr.

Thr Arg Asp Aia 100 Lys Val Gly 5 Asp Thr His Tyr Gin Asp Gin Asn His Tyr Val Asp Lys 70 Met Pro Ile Ser Arg 150 1446 WO 01/00843 WO 0100843PCTIIBOO/00923 Val Ala Leu Glu Thr Lys Leu Lys Ser Gly Asp Arg Val Giu Val Phe 165 170 175 Thr Ser Lys Asp Gin Asn Ala Gly Pro Ser Arg Gly Trp Gin Giu Phe 180 185 190 Val Val Ser Pro Arg Ala Lys Ala Lys Ile Arg Gin Trp Phe Ala Lys 195 200 205 Giu Arg Arg Glu Giu Tyr Leu Giu Ala Gly Arg Asp Ala Leu Ala Ala 210 215 220 Val Ile Gin Arg Gly Gly Leu Pro Met His Arg Leu Phe Thr Ala Ser 225 230 235 240 Ser Met Lys Thr Val Ala Thr Gluf Leu His Tyr Pro Asp Val Asp Ala 245 250 255 Leu Tyr Thr Ala-Ile Gly Ser Gly Ser Val Ser Ala Gin His Val Val 260 265 270 Asn Arg Leu Met Ala Ile Phe Gly Asp Glu Giu Asp Ala Giu Asp Ala 275 280 285 Leu Val Ala Arg Thr Pro Phe Ser Giu Leu Val Asn Ser Arg Ala Thr 290 295 300 Thr Giu Ser Ser Thr Gly Ile Leu Val Giu Gly Ser Pro Asp Val Met 305 310 315 320 Ala Lys Leu Ala Lys Cys Cys Met Pro Val Pro Gly Asp Giu Ile Phe 325 330 335.

Gly Phe Val Thr Arg Gly Gly Gly Val Ser Val His Arg Thr Asp Cys 340 345 350 Thr Asn Val Giu Lys Leu Lys Glu Giu Pro Giu Arg Ile Val Ser Val 355 360 365 Ser Trp Ala Ser Glu Gly Gin Gly Ser Val 370 375 <210> 1057 <211> 1059 <212> DNA <213> Corynebacteriun giutanicuxn <22 0> <221> CDS <222> (101)..(1036) <223> RXN01940 <400> 1057 ccagaatcaa tcaccgatac agtggcagcc aaagtccagg ctgaagaagc cgctcaagcc gccagctaaa tccacaaact gaactaagga gttttaccct atg acc acc aag atc 115 Met Thr Thr Lys Ile 1 1447 WO 01/00843 atc ctc gac Ile Leu Asp gca gcc ggc Ala Ala Giy ggc aac cag Gly Asn Gin acc atc gct Thr Ile Ala ttg gtg cgc Leu Val Arg atg gaa atc met Giu Ilie gac acc cac Asp Thr His ccc ggc agc Pro Giy Ser 120 ctg gca gtc Leu Ala Val 135 gtc ctc atg Vai Leu Met 150 gaa ttc aac Giu Phe Asn gaa aag tgg Giu Lys Trp ctc gca aca Leu Ala Thr 200 gtc gcc gac Vai Ala Asp 215 cag gac gca Gin Asp Ala 230 cca gga cac Pro Gly His gaa att gaa Giu Ile Giu gac aag gtc Asp Lys Vai 45 aat gcg ccc Asn Ala Pro gag gta gcc Giu Vai Ala 75 tac gaa ctg Tyr Giu Leu gat ttc atc Asp Phe Ile ctg gtt ccc Leu Val Pro 125 gaa cca cgc Giu Pro Arg 140 ggc tac cac Gly Tyr His 155 atc gac ccc Ile Asp Pro act atg gtc Thr Met Val atc gaa gcc Ile Giu Ala 205 gtc gcg ctt Val Ala Leu 220 ttt gat aac Phe Asp Asn 235 gac Asp ctg Leu acc Thr atc Ilie gaa Giu cct Pro atc Ile 110 acc Thr atc Ile gta Val gaa Giu Gly 190 aag Lys ttc Phe cca Pro gac Asp ctt Leu cac His tac Tyr gat Asp gaa Giu gat Asp gga Gly gcc Ala gga Gly gca Ala 175 ctc Leu ttc Phe gac Asp c ca Pro gcc Ala acc Thr cag Gin gtc Val ggc Gly aag Lys atg Met acc Thr 130.

gtc Val acc Thr atc Ile acc Thr ctg Leu 210 cgc Arg gac Asp atg Met acg Thr gtc Val1 acc Thr ga t Asp cag Gin aat Asn 115 aac Asn aag Lys gcc Ala gta Val cac His 195 ggc Gly aag Lys cc t Pro PCTJIBOO/00923 ctg ptc 163 Leu Leu gtc ggc 211 Val Gly gta gcc 259 Val Ala cga cca 307 Arg Pro acc ggc 355 Thr Gly gta gaa 403 Val Giu 100 aac gag 451 Asn Giu atc gcg 499 Ile Ala gaa gtt 547 Giu Val gta gct 595 Val Ala 165 ttc aac 643 Phe Asn 180 cag gcg 691 Gin Ala acc gac 739 Thr Asp aat tac 787 Asn Tyr tgt gct 835 Cys Ala 245 gtt gca tac ctt gtt gac cca acc gta ttc acc acc cgc aaa gca cca 1448 WO 01/00843 Val Ala Tyr Leu Val Asp Pro Thr Val Phe 250 255 ctc gat gtg gag ctg tac ggc gca ctc acc Leu Asp Val Glu Leu Tyr Gly Ala Leu Thr 265 270 gat ttc cgc gca ccg gct cca gca gat tgc Asp Phe Arg Ala Pro Ala Pro Ala Asp Cys 280 285 gac ctg gac ttt gat aaa ttc tgg aac atg 1027 Asp Leu Asp Phe Asp Lys Phe Trp Asn Met 295 300 cgc atc gga tagacctgtt cacaaggttg tta 1059 Arg Ile Gly 310 <210> 1058 <211> 312 <212> PRT <213> Corynebacterium giutamicum <400> 1058 met Thr Thr Lys Ile Ile Leu Asp Cys Asp

PCTLB

La Pro Thr Thr Arg Lys 00/00923 931 979 aca Thr acc Thr gtg Val1 ggC Gly acc Thr atc Ile 305 atg acc gtt gct Met Thr Val Ala 275 caa gta gct gtt Gin Val Ala Val 290 gat gca gta aag Asp Ala Val Lys 1 Val Ile Thr Gly His Thr Ile Leu Arg 145 Trp Met' Thr Val Thr Asp Gin Asn 115 Asn Lys Ala Leu Val Val1 Arg Thr Val 100 Asn Ile Glu Val 5 Leu Gly Ala Pro Gly Glu Glu Ala Val Ala 165 Gly Ile Lys Ala Val Giu Phe Val1 Pro 140 Tyr Asp His Glu Val Pro Ala Leu Ile Pro 125 Arg His Pro Asp Leu Thr Ile Giu Pro Ile 110 Thr Ile Val Glu Asp Leu His Tyr Asp Giu Asp Gly Ala Gly Ala 175 1449 WO 01/00843 His Ile Val Phe Asn Giu Lys Trp 180 Leu Thr His Gin Ala Leu Ala Thr 195 200 Glu Leu Gly Thr Asp Val Ala Asp 210 215 Phe Arg Lys Asn Tyr Gin Asp Ala 4 225 230 His Asp Pro Cys Ala Val Ala Tyr 245 Thr Arg Lys Ala Pro Leu Asp Val 260 Gly Met Thr Val Ala Asp Phe Arg 275 280 Thr Gin Val Ala Val Asp Leu Asp 290 295 Ile Asp Ala Val Lys Arg Ile Gly 305 310 <210> 1059 <211> 602 <212> DNA <213> Corynebacterium giutamicum <220> <221> CDS <222> <223> FRXA01940 <400> 1059 aga gta acg ctt gtt tcc acc ggg Arg Val Thr Leu Val Ser Thr Gly 1 5 gtc cgg aaa gaa cca cgc atc gcc Val Arg Lys Glu Pro Arg Ile Ala atg ggc ggg ggc tac cac gta gga Met Gly Gly Gly Tyr His Val Gly 40 aac atc aag atc gac ccc gaa gca Asn Ile Lys Ile Asp Pro Giu Ala 55 tgg cca ctg act atg gtc ggc ctc Trp Pro Leu Thr Met Val Gly Leu aca cct gag atc gaa gcc aag ttc Thr Pro Giu Ile Giu Ala Lys Phe PCTIBOO/00923 Pro 185 Pro Phe Gln Leu lu 265 Ala Phe cca Pro gag Glu 25 aac ksn cC 4 9ia I gac aac ksn 4 1450 Leu lu Val ly Val 250 Leu Pro Asp ctg Leu 10 cga Arg tgg rrp cac His ctt Leu gag lu Met Glu Ala 220 Asp Pro Gly Pro Phe 300 aac Asn aag Lys gcc Ala gta Val cac His ggc Gly Val Ala 205 Leu Asn Thr Ala Ala 285 Trp atc Ile gaa Glu gta Val ttc Phe cag Gin acc Thr Gly 190 Lys Phe Pro Val Leu 270 Asp Asn gcg ctg Ala Leu gtt gtc Val Val gct gaa Ala Glu aac gaa Asn Glu gcg ctc Ala Leu gac gtc Asp Val WO 01/00843 WO 0100843PCTIIBOO/00923 gac Asp gca Ala tac Tyr gtg Val 145 cgc Arg gac Asp gga Gly ttc gtc gtc gcg ctt ttc gac Phe Val Val Ala Leu Phe Asp 100 cag ggt ttt gat aac cca cca Gin Gly Phe Asp Asn Pro Pro 115 120 ctt gtt gac cca acc gta ttc Leu Val Asp Pro Thr Val Phe 130 135 gag ctg tac ggc gca ctc acc Glu Leu Tyr Gly Ala Leu Thr 150 gca ccg gct cca gca gat tgc Ala Pro Ala Pro Ala Asp Cys 165 ttt gat aaa ttc tgg aac atg Phe Asp Lys Phe Trp Asn Met 180 tagacctgtt cacaaggttg tta cgc Arg gac Asp cgc Arg atg Met 155 c aa Gin aag Lys cc t Pro aaa Lys 140 acc Thr gta Val gat gca gta Asp Ala Val <210> 1060 <211> 193 <212> PRT <213> Corynebacterium glutamicun <400> 1060 Arg Val Thr Leu Val Ser Thr Gly Pro 1 Val Arg Lys Giu Pro Arg Ile Ala Glu 25 Met Gly Gly Gly Tyr His Val Gly Asn 40 Asn Ile Lys Ile Asp Pro Glu Ala Ala 55 Trp Pro Leu Thr Met Val Gly Leu Asp Thr Pro Glu Ile Glu Ala Lys Phe Asn Asp Phe Val Val Ala Leu Phe Asp Ala 100 105 Ala Gin Gly Phe Asp Asn Pro Pro Val 115 120 Asn Lys Ala Val1 His Gly Lys Pro Ile Ala Glu Val Val Ala Phe Asn Gin Ala Thr Asp Asn Tyr 110 Cys Ala 125 1451 WO 01/00843 PCTIBOO/00923 Tyr Leu Val Asp Pro Thr Val Phe Thr Thr Arg Lys Ala Pro Leu Asp 130 135 140 Val Glu Leu Tyr Gly Ala Leu Thr Thr Gly Met Thr Val Ala Asp Phe 145 150 155 160 Arg Ala Pro Ala Pro Ala Asp Cys Thr Thr Gin Val Ala Val Asp Leu 165 170 175 Asp Phe Asp Lys Phe Trp Asn Met Val Ile Asp Ala Val Lys Arg Ile 180 185 190 Gly <210> 1061 <211> 1026 <212> DNA <213> Corynebacteriun glutamicun <220> <221> CDS <222> (101)..(1003) <223> RXA02559 <400> 1061 ttagtcctct atggcagcaa gcctgccaga ggagcctatc cagcactaga ccccaactag aacccaaccc caaaaccaga aaacactaag ctcgtgagac atg att cct gtt ctc Met Ile Pro Val Leu atc Ile gtt Val acc Thr gtg Val cct Pro gac Asp ggt Gly gac Asp cat His aat Asn cag Gin aag Lys ctt Leu aag Lys 105 ggc Gly cgt Arg gat Asp gga Gly gtg Val tat Tyr ctg Leu 1 ctc gcc Leu Ala ctt ttt Leu Phe gcc atc Ala Ile atc Ile gca Ala acc Thr gca Ala aca Tht att Ile cca Pro 115 tac Tyr acg Thr agg Arg gga Gly cac His c ca Pro 100 gaa Glu 115 163 211 259 307 355 403 451 1452 WO 01/00843 aag ctg att Lys Leu Ilie 120 PCT/IBOO/00923 gtc acc ggg ccc Val Thr Gly Pro gcc acc Ala Thr 125 aac ctt gcg Asn Leu Ala gaa Giu 130 ttc ggg cca Phe Gly Pro gtg gaa Val Giu 135 aac gtc acg ctg atg ggt Asn Val Thr Leu Met Gly ggc acc tac Gly Thr Tyr ctt Leu 145 tat cca ggc aac Tyr Pro Gly Asn acc Thr 150 act cca acg gca Thr Pro Thr Ala gaa Giu 155 tgg aat acc tgg Trp Asn Thr Trp gtt Val 160 gat cca cac gga Asp Pro His Gly aaa gaa gca ttc Lys Giu Ala Phe gca gcc caa aag Ala Ala Gin Lys att acg gtg tgt Ile Thr Val Cys tcc ttg Ser Leu 180 ggc gtg acc Gly Val Thr atc aac acg Ilie Asn Thr -200 gag Giu 185 cag ttt acg ctg Gin Phe Thr Leu ccg gac atc ctt Pro Asp Ile Leu tct aca ctt Ser Thr Leu 195 cct gag atg Pro Giu Met ctt ggc agc caa Leu Gly Ser Gin atc gca gag cat Ile Ala Giu His tta Leu 210 ctg cgc Leu Arg 215 ttt tac ttt gaa Phe Tyr Phe Giu tt t Phe 220 cac gaa gtg cag His Giu Val Gin ggc Gly 225 gaa ggt tac ctt Giu Gly Tyr Leu caa att cat gac Gin Ile His Asp ctg Leu 235 ctg acc tgc atg Leu Thr Cys Met att Ile 240 gcc ttg gat aaa Ala Leu Asp Lys atc Ile 245 cca ttt tca ggc Pro Phe Ser Gly cgt Arg 250 gaa gta acc gtg Glu Val Thr Val gtg gag gct gat Val Giu Ala Asp tcg ccc Ser Pro 260 ttg atg cgt Leu Met Arg cca gct aac Pro Ala Asn 280 ggc Gly 265 acc act gtt gca Thr Thr Val Ala att cgc gga cat Ile Arg Gly His tgg ggc aag Trp Gly Lys 275 gcc gcc cac Ala Ala His gca ttt ctt gtg Ala Phe Leu Val acc gca gac att Thr Ala Asp Ilie gag Giu 290 gcg gaa ct 1026 Ala Giu Le 295 <210> 1062 <211> 301 <212> PRT t cta aga gca gtg gaa tgaaataatc cggtgctgat gca u Leu Arg Ala Val Giu 300 <213> Corynebacterium glutamicum <400> 1062 Met Ile Pro Val Leu Ile Asp Cys Asp Thr Gly Ile Asp Asp Ala Leu Ala Leu Ile Tyr Leu Val Ala Leu His Lys Arg Gly Giu Ile Gin Leu 25 1453 WO 01/00843 WO 0100843PCTLBOO/00923 Phe Gly Ala Thr Thr Thr Ala Gly Asn Val Asp Val Lys Gin Thr Ala 40 Ile Asn Thr Arg Trp Val Leu Asp Gin Cys Giy Leu Ala Asp Ile Pro 55 Val Leu Ala Gly Gin Pro Glu Pro Lys His Val Pro Leu Val Thr Thr 70 75 Pro Giu Thr His Gly Asp His Gly Leu Gly Tyr Ile Asn Pro Gly His 90 Val Glu Ilie Pro Glu Giy Asp Trp Lys Gin Leu Trp Lys Glu His Leu 100 105 110 Ser Asn Pro Giu Thr Lys Leu Ilie Val Thr Gly Pro Ala Thr Asn Leu 115 120 125 Ala Giu Phe Giy Pro Vai Glu Asn Val Thr Leu Met Giy Gly Thr Tyr 130 135 140 Leu Tyr Pro Giy Asn Thr Thr Pro Thr Ala Glu Trp Asn Thr Trp Val 145 150 155 160 Asp Pro His Gly Ala Lys Giu Ala Phe Ala Ala Ala Gin Lys Pro Ile 165 170 175 Thr Val Cys Ser Leu Gly Vai Thr Glu Gin Phe Thr Leu Asn Pro Asp 180 185 190 Ile Leu Ser Thr Leu Ile Asn Thr Leu Gly Ser Gin Pro Ile Ala Glu 195 200 205 His Leu Pro Giu Met Leu Arg Phe Tyr Phe Glu Phe His Glu Val Gin 210 215 220 Gly Glu Gly Tyr Leu Ala Gin Ile His Asp Leu Leu Thr Cys Met Ile 225 230 235 240 Ala Leu Asp Lys Ile Pro Phe Ser Giy Arg Glu Val Thr Val Asp Val 245 250 255 Giu Ala Asp Ser Pro Leu Met Arg Gly Thr Thr Val Ala Asp Ile Arg 260 265 270 Gly His Trp Gly Lys Pro Ala Asn Ala Phe Leu Val Giu Thr Ala Asp 275 280 285 Ile Glu Ala Ala His Ala Glu Leu Leu Arg Ala Val Glu 290 295 300 <210> 1063 <211> 1050 <212> DNA <213> Corynebacterium giutamicun <22 0> <221> CDS <222> (101)..(1027) 1454 WO 01/00843 WO 0100843PCTIBOO/00923 <223> RXA02497 <400> 1063 tcgatgccgc cgctggcgaa gactcgggga aacctaaaaa gattcacact ttgccaccct agaccgtcta acctttaggt taccgaagaa gaatttgacc gtg aga tta ggt gta ii! Val Arg Leu Gly Val tta gat gtg ggc Leu Asp Val Gly aat act gtc cac cta gtt gca gta gac Asn Thr Val His Leu Val Ala Val Asp gcg cgt Ala Arg ccc ggt gga Pro Gly Gly ctt gtt gag Leu Val Giu ccc acc ccg atg agc aat tgg cgt acc Pro Thr Pro Met Ser Asn Trp Arg Thr cca ctg cgc Pro Leu Arg aag ggc atc Lys Gly Ile ctt ctt gat gac Leu Leu Asp Asp tcc Ser ggg gcg atc tcc Gly Ala Ile Ser aac aaa Asn Lys ctc acc tca gca gtc ggg gaa gca gca Leu Thr Ser Ala Val Gly Glu Ala Ala cta gcg aaa acg Leu Ala Lys Thr 307 355 ctc Leu ggc tgc gct gaa Gly Cys Ala Glu atg cca ttt gct aca tcg gca gtc cgc Met Pro Phe Ala Thr Ser Ala Val Arg 80 tcc Ser gcc acc aac agc Ala Thr Asn Ser gca gtg ctc gac Ala Val Leu Asp gtg gag aag gaa Val Glu Lys Glu acc ggc Thr Gly 100 gtC cgc ctg Val Arg Leu ctc gca gtt Leu Ala Val 120 atc ctt tcc ggt Ile Leu Ser Gly gaa Glu 110 gac gaa gca cgc Asp Glu Ala Arg caa act ttc Gin Thr Phe 115 ata act aac Ile Thr Asn 451 499 cga cgt tgg tat Arg Arg Trp Tyr gga Gly 125 tgg tcc gca ggg Trp, Ser Ala Gly cgc Arg 130 ctc gac Leu Asp 135 atc ggt ggc ggc Ile Gly Gly Gly ctg gaa cta tcc tcc gga acc gac gaa Leu Giu Leu Ser Ser Gly Thr Asp Giu 145 tcC Ser 150 cca gac ctc gcg Pro Asp Leu Ala tca ctg gat ctg Ser Leu Asp Leu ggt Gly 160 gcg ggc cgc ttg Ala Gly Arg Leu cac aac tgg ttc His Asn Trp, Phe acc gat cca ccg Thr Asp Pro Pro gca Ala 175 cgt aag aaa atc Arg Lys Lys Ile aac ctc Asn Leu 180 ctg cgc gat Leu Arg Asp cgc acc cta Arg Thr Leu 200 tat Tyr 185 atc gat gcg gaa Ile Asp Ala Glu gca gaa ccc gcc Ala Glu Pro Ala cgc cag atg Arg Gin Met 195 aaa act ttc.

Lys Thr Phe ggg ccc gcg cgc Gly Pro Ala Arg gca gtg gga aca Ala Val Gly Thr tcc Ser 210 1455 WO 01/00843 WO 0100843PCTIIIBOO/00923 cgc acc ctg gca cga ctg act ggt gct Arg Thr Leu Ala Arg Leu Thr Gly Ala 215 220 cac gtc acc cga acc ctc acc gcg ccg His Val Thr Arg Thr Leu Thr Ala Pro 230 235 ttt atc tca cga atg act gcg gcg gac Phe Ile Ser Arg Met Thr Ala Ala Asp 250 agc tcg gat cgg tca cat cag atc gtg Ser Ser Asp Arg Ser His Gin Ilie Val 265 270 gct gcg atg cgt gcg ttg gat att gac Ala Ala Met Arg Ala Leu Asp Ilie Asp 280 285 gca ctt cgt gaa ggt gtg atc ctc acc 1027 Ala Leu Arg Glu Gly Val Ilie Leu Thr 295 300 taacatttac ccggaaagga gtt 1050 <210> 1064 <211> 309 <212> PRT <213> Corynebacterium giutamicun <400> 1064 Val Arg Leu Gly Val Leu Asp Val Gly 1 Ala Val Asp Ala Arg Pro Gly Gly His Arg Thr Pro Leu Arg Leu Val Giu Leu 40 Ser Glu Lys Gly Ile Asn Lys Leu Thr 55 Asp Leu Ala Lys Thr Leu Gly Cys Ala Ser Ala Val Arg Ser Ala Thr Asn Ser Glu Lys Glu Thr Gly Val Arg Leu Ser 100 105 Ala Arg Gin Thr Phe Leu Ala Val Arg 115 120 Gly Arg Ilie Thr Asn Leu Asp Ile Gly 130 135 ccc Pro ctg Leu 240 gct Ala ggt Gly gta Val1 gga cca Gly Pro atc gca Ile Ala 245 ggt atc Gly Ile 260 gcg gaa Ala Glu ccg tgg Pro Trp ctc gag Leu Glu 787 835 883 931 979 gaa att Glu Ile 290 gac aaa Asp Lys 305 agg atc Arg Ile Thr Pro Asp Val Met Val Ser Tyr Ser 140 Val Met Ser Gly Pro Leu Gly Gly 125 Leu 1456 WO 01/00843 WO 0100843PCTA~BOO/00923 Ser Gly Thr Asp Glu Ser Pro Asp Leu Ala Phe 145 150 155 Ala Gly Arg Leu Thr His Asn Trp Phe Asp Thr 165 170 Lys Lys Ile Asn Leu Leu Arg Asp Tyr Ile Asp 180 185 Pro Ala Arg Gin met Arg Thr Leu Gly Pro Ala 195 200 Thr Ser Lys Thr Phe Arg Thr Leu Ala Arg Leu 210 215 Ser Ser Ala Gly Pro His Val Thr Arg Thr Leu 225 230 235 Arg Gin Leu Ile Aia Phe Ile Ser Arg Met Thr 245 250 Giu Leu Glu Gly Ilie Ser Ser Asp Arg Ser His 260 265 Ala Leu Val Ala Glu Ala Aia Met Arg Ala Leu 275 280 Giu Ile Cys Pro Trp Ala Leu Arg Giu Gly Val 290 295 Asp Lys Gly Leu Giu 305 <210> 1065 <211> 2226 <212> DNA <2i3> Corynebacteriun giutamicun <22 0> <221> CDS <222> (101)..(2203) <223> RXN01079 <400> 1065 accttaaaac ttaatcaatc aatacaaagt tcacacctct gactgcatcg ggaaccaccg tcgtgccacg cggagaccag ctt aac gcg ttg ctc aac ctt tac gat gac aac Leu Asn Ala Leu Leu Asn Leu Tyr Asp Asp Asn 15 gag aaa gac cgt gaa gct gca aac cag tac ttc Glu Lys Asp Arg Glu Ala Ala Asn Gin Tyr Phe 30 cag aac acc gtc ttc ttc cac aac ctg cag gaa 1457 Ser Asp Aia Arg Thr 220 Thr Ala Gin Asp Ile 300 Gly 160 Arg Giu Gly Pro Leu 240 Ala Gly Val Ile tttgaaaggg acattccttt atg gac ttc cac gct Met Asp Phe His Ala 1 ggc aag atc cag ttt Gly Lys Ile Gin Phe ctg cag cac gtc aac Leu Gin His Val Asn aag atc gac tac ctg WO 01/00843 Gin Asn Thr PCTIiBOO/00923 Val Phe Phe His Leu Gin Giu Lys Asp Tyr Leu gtt gaa Val Giu aac aag tac tat Asn Lys Tyr Tyr cca atc gtt ctg Pro Ile Val Leu aag tac gac ttc Lys Tyr Asp Phe cag Gin ttc atc aag gac Phe Ile Lys Asp ttc aag cgc gca Phe Lys Arg Aia gga ttc aag ttc Giy Phe Lys Phe cgc Arg ttc cag tcc ttc Phe Gin Ser Phe ggt gca tac aag Giy Aia Tyr Lys tac act tcc tac Tyr Thr Ser Tyr acc ctg Thr Leu 100 aag acc ttc Lys Thr Phe tgc atg gtc Cys Met Vai 120 gac Asp 105 ggt cgc cgc tac Gly Arg Arg Tyr gag cgt ttc gaa Giu Arg Phe Giu gac cgt gtc Asp Arg Val 115 ttg gcc gag Leu Ala Giu gcc ctc acc ctc Ala Leu Thr Leu gac ggc gac cgc Asp Giy Asp Arg aac ctg Asn Leu 135 gtc gat gag atc Vai Asp Giu Ile tct ggc cgt ttc Ser Giy Arg Phe caa cca gca acc cca Gin Pro Aia Thr Pro 145 gag cca gta tcc tgc Giu Pro Val Ser Cys ttc ctg aac tcc Phe Leu Asn Ser aag gca cag cgC Lys Aia Gin Arg ttc ctc ctg cgt Phe Leu Leu Arg gaa gac aac atg Giu Asp Asn Met tcc atc gga cgt Ser Ilie Giy Arg tcc atc Ser Ile 180 aac tct gct Asn Ser Ala ctg tcc aac Leu Ser Asn 200 ctt Leu 185 cag ctg tcc aag Gin Leu Ser Lys ggc ggt ggc gta Giy Gly Gly Vai gcg ttg ctg Ala Leu Leu 195 att gaa aac Ile Giu Asn ctt cgt gaa gcc Leu Arg Giu Aia gca ccg att aag Ala Pro Ile Lys aag Lys 210 cag tct Gin Ser 215 tcc ggt gtt atc Ser Gly Val Ile gtg atg aaa ctt Vai Met Lys Leu gaa gat gct ttc Giu Asp Aia Phe tac gct aac cag Tyr Ala Asn Gin ggt gct cgt cag Gly Aia Arg Gin gca ggt gct gtg Aia Giy Ala Vai ctc aac gct cac Leu Asn Ala His c ac His 250 cca gat atc ctg Pro Asp Ile Leu ttc ctg gat acc Phe Leu Asp Thr aag cgt Lys Arg 260 gag aac gcc Giu Asn Aia gat Asp 265 gag aag atc cgc Giu Lys Ile Arg aag acc ctg tcc Lys Thr Leu Ser ctg ggt gtt Leu Giy Vai 275 gtg att ccg gac atc acc ttc gag ctg gct aag cgc aac gat gac atg Vai Ile Pro Asp Ile Thr Phe Glu Leu Ala Lys Arg Asn Asp Asp Met 1458 WO 01/00843 280 tac ctg ttc 1027 Tyr Leu Phe 295 gca gac gtc 1075 Ala Asp Val 310 cgc atc cgc 1123 Arg Ile Arg PCTIBOO/00923 tcc cca tac gat Ser Pro Tyr Asp 300 tca atc acc gag Ser Ile Thr Giu gag cgc att Giu Arg Ile aag acc Lys Thr 315 aag Lys cac tac gac gag His Tyr Asp Glu 320 aac gcg cgt cag Asn Ala Arg Gin atc Ile 290 tac ggc aag cct ttc Tyr Giy Lys Pro Phe 305 atg gtg gat gat gac Met Vai Asp Asp Asp 325 ttc ttc cag acc ctg Phe Phe Gin Thr Leu 340 gca gaa 1171 Ala Giu acc gtg 1219 Thr Vai ctg tgc 1267 Leu Cys 375 gac ctg 1315 Asp Leu 390 tcc ctc 1363 Ser Leu atc gaa 1411 Ilie Giu atc gat 1459 Ile Asp atc ggc 1507 Ile Gly 455 atg cac 1555 Met His 470 330 atc cag ttc Ilie Gin Phe 345 aat gca tcc Asn Ala Ser gag tcc ggt Glu Ser Gly aac cca atc Asn Pro Ile 365 ctt cag gtg Leu Gin Val tac cca tac atc atg tat gaa gac Tyr Pro Tyr Ile met Tyr Giu Asp 350 355 gaa ggt cgc atc acc cac tca aac Giu Gly Arg Ile Thr His Ser Asn 370 tcc acc cca tct gaa ttc aac gat Ser Thr Pro Ser Giu Phe Asn Asp 385 gaa gac att tct tgt aac ttg ggt Giu Asp Ile Ser Cys Asn Leu Gly 335 gag atc Giu Ilie 380 act tac gca gag Thr Tyr Ala Giu 395 aac gtt gca atg Asn Vai Ala Met 410 gtc ggc Val Giy 400 gct atg gat gca cca Ala met Asp Aia Pro 415 aac ttt gag aag acc Asn Phe Giu Lys Thr 420 acc gaa Thr Giu 425 tcc gtg Ser Val atc cgc ggc tta act Ile Arg Gly Leu Thr 430 cct tcc atc cgt aag Pro Ser Ile Arg Lys 445 cag atg aac ctt cac Gin Met Asn Leu His gca gtg cct gag cag acc agc Ala Val Pro Giu Gin Thr Ser 435 ggc aac gaa gca gct cac gcc Gly Asn Giu Ala Ala His Ala 450 ggc tac ttc ggt cgc gag cac Gly Tyr Phe Gly Arg Giu His ggc Gly tac Tyr 460 ggc tcc gag gaa Gly Ser Giu Giu 465 gcc ctg gac ttc acc Ala Leu Asp Phe Thr 480 aac gca tac ttt Asn Ala Tyr Phe 485 475 1459 WO 01/00843 WO 0100843PCTIIBOOIOO923 gct gcc 1603 Ala Ala gag cgt 1651 Giu Arg ggt gag 1699 Gly Giu gac aag 1747 Asp Lys 535 gtt gag 1795 Val Giu 550 ttc aac 1843 Phe Asn atc aac 1891 Ile Asn atc cgc 1939 Ile Arg atg gac 1987 Met Asp 615 tac gag 2035 Tyr Giu 630 cag ggc 2083 Gin Gly gac atc 2131 Asp Ile gtg ctg tac Val Leu Tyr 490 gga gag Gly Giu 505 tac ttc Tyr Phe 520 cgt Arg ga t Asp cag tgc ctg cgt gca Gin Cys Leu Arg Ala 495 ttc aag aac ttc gaa Phe Lys Asn Phe Giu 510 gat ttc gat gca aac Asp Phe Asp Ala Asn tcc aac cag atc gct act Ser Asn Gin Ile Ala Thr 500 aac tcc aag tat gca acc Asn Ser Lys Tyr Ala Thr 515 gac ttc gca cca aag tcc Asp Phe Ala Pro Lys Ser 530 aac atc cac acc cca acc Asn Ile His Thr Pro Thr 525 gtc aag gaa ctc Val Lys Glu Leu gac tgg gct gcg Asp Trp Ala Ala 555 cgt aac ctg caa Arg Asn Leu Gin 570 t tt Phe 540 gcc Ala aag tcg Lys Ser 545 ctg aag gcc gac gtg Leu Lys Ala Asp Val 560 gcg gtt cca cca acc Ala Val Pro Pro Thr 575 tcg atc cac cca atc Ser Ile His Pro Ile atg Met gag cac ggt ctg Giu His Gly Leu 565 aac tcc Asn Ser 585 aag gaa Lys Glu acc tcg ggt tcg atc tcc tac Gly Ser Ile Ser Tyr 580 gca tcc aag att gag Ala Ser Lys Ile Giu 595 ggc aag atc ggc Gly Lys Ile Gly 600 590 aat gac aac ctt gag Asn Asp Asn Leu Glu 620 aag atc att gac acc Lys Ile Ile Asp Thr 635 cgc gtt tac tac cca gct cca cac Arg Val Tyr Tyr Pro Ala Pro His 610 ttC gag gac gcc tac gaa atc ggc Phe Giu Asp Ala Tyr Giu Ile Gly 625 gct gtg gca acc aag tac gtt gac Ala Val Ala Thr Ly's Tyr Val Asp tac Tyr ctg tca ctg acc ttg ttc ttc aag Leu Ser Leu Thr Leu Phe Phe Lys 650 655 aac cgt gcg cag atc tac gca tgg Asn Arg Ala Gin Ile Tyr Ala Trp 665 670 640 645 gac act gcc acc acc. cgt Asp Thr Ala Thr Thr Arg cgc aag ggc atc Arg Lys Gly Ile 675 660 aag acc Lys Thr 1460 WO 01/00843 WO 0100843PCTIiBOO/00923 ttg tac tac att'cgc ctg cgc cag 2179 Leu Tyr Tyr Ile Arg Leu Arg Gin 680 685 gac ggc tgc gtc agc tgc atg ctg 2226 Asp Gly Cys Val Ser Cys Met Leu 695 700 <210> 1066 <211> 701 <212> PRT <213> Corynebacteriun giutamicun <400> 1066 Met Asp Phe His Ala Leu Asn Ala gtt gct ctg gaa ggc act gaa 4tt Val Ala Leu Glu Gly Thr Giu Vai 690 taaaagcact taaaaatatc ccc 1 Gly Leu Lys Asp G ly Thr Phe Arg Gin 145 Giu Ile Gly Lys Leu 225 Giu Gin Val1 Gin 70 Phe Lys Cys Asn Thr 150 Phe Asn Leu Gin Ser 230 Lys Asn Giu Phe Gin Thr Met Leu 135 Phe Leu Ser Ser Ser 215 Tyr Asn Ala Phe Tyr Leu.

75 Giy Arg Thr Ile Gly 155 Glu Leu Giu Ile Leu 235 Tyr Asn Asn Pro Lys Tyr Tyr ,Ala 125 Ser Ala Asn Lys Gly 205 Val Ala Asp Gin Leu Ile Arg Lys Leu 110 Asp Gly Gin Met Arg 190 Ala Met Arg Asp Tyr Gin Val Ala Tyr Giu Gly Arg Arg Glu 175 Gly Pro Lys Gin Asn Phe Giu Leu Tyr Tyr Arg Asp Phe Gly 160 Ser Gly Ile Leu Gly 240 1461 WO 01/00843 WO 0100843PCT/IBOO/00923 Ala Gly Ala Val Tyr Leu Asn Ala His His Pro Asp Ile Leu Ser Phe 245 250 255 Leu Asp Thr Lys Arg Giu Asn Ala Asp Giu Lys Ile Arg Ile Lys Thr 260 265 270 Leu Ser Leu Gly Val Vai Ile Pro Asp Ile Thr Phe Giu Leu Ala Lys 275 280 285 Arg Asn Asp Asp Met Tyr Leu Phe Ser Pro Tyr Asp Val Giu Arg Ile 290 295 300 Tyr Gly Lys Pro Phe Ala Asp Val Ser Ile Thr Giu His Tyr Asp Glu 305. 310 315 320 Met Val Asp Asp Asp Arg Ile Arg Lys Thr Lys Ile Asn Ala Arg Gin 325 330 335 Phe Phe Gin Thr Leu Ala Giu Ilie Gin Phe Giu Ser Giy Tyr Pro Tyr 340 345 350 Ile Met Tyr Giu Asp Thr Vai Asn Ala Ser Asn Pro Ile Giu Gly Arg 355 360 365 Ile Thr His Ser Asn Leu Cys Ser Giu Ile Leu Gin Vai Ser Thr Pro 370 375 380 Ser Giu Phe Asn Asp Asp Leu Thr Tyr Ala Giu Vai Gly Giu Asp Ile 385 390 395 400 Ser Cys Asn Leu Gly Ser Leu Asn Val Aia Met Ala Met Asp Ala Pro 405 410 415 Asn Phe Giu Lys Thr Ilie Giu Thr Giu Ile Arg Gly Leu Thr Ala Val 420 425 430 Pro Giu Gin Thr Ser Ilie Asp Ser Val Pro Ser Ile Arg Lys Gly Asn 435 440 445 Giu Ala Aia His Ala Ile Gly Leu Giy Gin met Asn Leu His Gly Tyr 450 455 460 Phe Gly Arg Giu His Met His Tyr Gly Ser Giu Giu Ala Leu Asp Phe 465 470 475 480 Thr Asn Aia Tyr Phe Ala Ala Vai Leu Tyr Gin Cys Leu Arg Ala Ser 485 490 495 Asn Gin Ile Ala Thr Giu Arg Gly Giu Arg Phe Lys Asn Phe Giu Asn 500 505 510 Ser Lys Tyr Ala Thr Gly Giu Tyr Phe Asp Asp Phe Asp Aia Asn Asp 515 520 525 Phe Ala Pro Lys Ser Asp Lys Val Lys Giu Leu Phe Ala Lys Ser Asn 530 535 540 Ile His Thr Pro Thr Vai Giu Asp Trp Ala Ala Leu Lys Ala Asp Val 545 550 555 560 1462 WO 01/00843 Met Giu His Gly Leu Phe Asn Arg Asn Leu Gin 565 570 Gly Ser Ile Ser Tyr Ile Asn Asn Ser Thr Ser 580 585 Ala Ser Lys Ile Giu Ile Arg Lys Giu Giy Lys 595 600 Tyr Pro Ala Pro His Met Asp Asn Asp Asn Leu 610 615 Ala Tyr Giu Ile Gly Tyr Glu Lys Ile Ile Asp 625 630 635 Thr Lys Tyr Val Asp Gin Giy Leu Ser Leu Thr 645 650 Thr Ala Thr Thr Arg Asp Ilie Asn Arg Ala Gin 660 665 Lys Giy Ile Lys Thr Leu Tyr Tyr Ile Arg Leu 675 680 Giu Giy Thr Giu Val Asp Giy Cys Vai Ser Cys 690 695 <210> i067 <211> 790 <2i2> DNA <213> Corynebacteriun giutanicun <220> <221> CDS <222> (101)..(790) <223> FRXA0i079 <400> 1067 accttaaaac ttaatcaatc aatacaaagt tcacacctct gactgcatcg ggaaccaccg tcgtgccacg cggagaccag Ala Ser Ile Giu 620 Thr Leu Ile Arg Met 700 Val Ile Giy 605 Tyr Tyr Phe Tyr Gin 685 Leu PCTIIBOO/00923 -o Thr :o Ile ~i Tyr Lu Asp il Ala 640 es Asp p Arg La Leu ctt Leu gag Giu cag Gin gtt Val ctc Leu gaa Glu t tc Phe tac Tyr aac Asn gct Ala t tc Phe tat Tyr ctt Leu gca Ala cac His gac Asp tac Tyr aac Asn aac Asn cca Pro tttgaaaggg acattccttt atg gac ttc cac gct Met Asp Phe His Ala 1 ggc aag atc cag ttt Giy Lys Ile Gin Phe ctg cag cac gtc aac Leu Gin His Vai Asn aag atc gac tac ctg Lys Ile Asp Tyr Leu gac aag tac gac ttc Asp Lys Tyr Asp Phe 115 163 211 259 307 1463 WO 01/00843 cag ttc at Gin Phe 11 ttc cag tc Phe Gin Se aag acc tt Lys Thr Ph tgc atg gt Cys Met Va 12 aac ctg gt Asn Leu Va 135 acc ttc ct Thr Phe Le 150 ttc ctc ct Phe Leu Le aac tct gc Asn Ser Al ctg tcc aa Leu Ser As

C

e

'C

r 1 0

C

1 g

U

g

U

t a

C

n 0 aag Lys ttc Phe gac Asp 105 gcC Ala ga t Asp aac Asn cgt Arg ctt Leu 185 ctt Leu tac Tyr 80 tac Tyr cgt Arg gac Asp t tc Phe ggc Gly 160 t CC Ser ggt Gly att Ile gga Gly act Thr t tC Phe cgc Arg caa Gin 145 gag Giu atc Ile ggc Giy aag Lys PCT/iBOO/00923 ttc cgc 355 Phe Arg acc ctg 403 Thr Leu 100 cgt gtc 451 Arg Val gcc gag 499 Ala Giu acc cca 547 Thr Pro tcc tgc 595 Ser Cys 165 tcc atc 643 Ser Ile 180 ttg ctg 691 Leu Leu gaa atc 739 Giu Ile cag tct tcc ggt gtt atc cca gtg atg aaa ctt ctg gaa gat gct ttc Gin Ser 215 tcc Ser 230 Ser Gly Val Ile Val Met Lys Leu Leu 225 Glu*Asp Ala Phe <210> 1068 <211> 230 <212> PRT <213> Corynebacteriun giutamicum <400> 1068 Met Asp Phe His Ala Leu Asn Ala Leu Leu Asn Leu Tyr Asp Asp Asn 1 5 10 Gly Lys Ile Gin Phe Giu Lys Asp Arg Giu Ala Ala Asn Gin Tyr Phe 25 Leu Gin His Vai Asn Gin Asn Thr Vai Phe Phe His Asn Leu Gin Glu 40 Lys le*Asp Tyr Leu Val Giu Asn Lys Tyr Tyr Asp Pro Ile Val Leu~ 1464 WO 01/00843 PCT/iBOO/00923 55 Asp Lys Tyr Asp Phe Gin Phe Ile Lys Asp Leu Phe Lys Arg Ala Tyr 70 75 Gly Phe Lys Phe Arg Phe Gin Ser Phe Leu Gly Alat Tyr Lys Tyr Tyr 90 Thr Ser Tyr Thr Leu Lys Thr Phe Asp Giy Arg Arg Tyr Leu Giu Arg 100 105 110 Phe Giu Asp Arg Val Cys Met Val Ala Leu Thr Leu Ala Asp Gly Asp 115 120 125 Arg Ala Leu Ala Giu Asn Leu Val Asp Giu Ilie Met Ser Gly Arg Phe 130 135 140 Gin Pro Ala Thr Pro Thr Phe Leu Asn Ser Gly Lys Ala Gln Arg Gly 145 150 155 160 Giu Pro Val Ser Cys Phe Leu Leu Arg Ile Glu Asp Asn Met Glu Ser 165 170 175 Ile Gly Arg Ser Ile Asn Ser Ala Leu Gin Leu Ser Lys Arg Gly Gly 180 185 190 Giy Vai Ala Leu Leu Leu Ser Asn Leu Arg Glu Ala Gly Ala Pro Ile 195 200 205 Lys Lys Ile Giu Ile Gin Ser Ser Gly Val Ile Pro Val Met. Lys Leu 210 215 220 Leu Glu Asp Ala Phe Ser 225 230 <210> 1069 <211> 1364 <212> DNA <213> Corynebacteriun giutamicum <22 0> <221> CDS <222> (1341) <223> FRXA01084 <400> 1069 tcc ttt ctg gat acc aag cgc gag aac gcc gat gag aag atc cgc atc 48 Ser Phe Leu Asp Thr Lys Arg Glu Asn Ala Asp Giu Lys Ile Arg Ile 1 5 10 aag acc ctg tcc ctg ggt gtt gtg att ccg gac atc acc ttc gag ctg 96 Lys Thr Leu Ser Leu Gly Val Val Ile Pro Asp Ile Thr Phe Glu Leu 25 gct aag cgc aac gat gac atg tac ctg ttc tcc cca tac gat gtg gag 144 Ala Lys Arg Asn Asp Asp Met Tyr Leu Phe Ser Pro Tyr Asp Val Giu 40 cgc att tac ggc aag cct ttc gca gac gtc tca atc acc gag cac tac 192 Arg Ile Tyr Gly Lys Pro Phe Ala Asp Val Ser Ile Thr Glu His Tyr 1465 WO 01/00843 WO 0100843PCTIBOOIOO923 gac Asp gag atg gtg gat Giu met Val Asp gac cgc atc cgc Asp Arg Ile Arg acc aag atc aac Thr Lys Ile Asn cgt cag ttc Arg Gin Phe cca tac atc Pro Tyr Ilie ggt cgc atc Giy Arg Ile 115 ttc Phe acc ctg gca gaa atc cag ttc gag tcc Thr Leu Aia Giu Ile Gin Phe Giu Ser ggt tac Gly Tyr 240 288 336 384 atg tat gaa gac acc gtg aat gca tcc aac cca atc gaa Met Tyr Giu Asp Thr Vai Asn Ala Ser Asn Pro Ile Giu 100 105 110 acc cac tca aac Thr His Ser Asn ctg Leu 120 tgc tct gag atc Cys Ser Giu Ile cag gtg tcc Gin Val Ser acc cca Thr Pro 130 tct gaa ttc aac Ser Giu Phe Asn gat Asp 135 gac ctg act tac Asp Leu Thr Tyr gag gtc ggc gaa Giu Vai Giy Giu gac Asp 145 att tct tgt aac Ile Ser Cys Asn ggt tcc ctc aac Giy Ser Leu Asn gca atg gct atg Ala Met Ala Met 432 480 528 gca cca aac ttt Ala Pro Asn Phe aag acc atn gaa acc gaa atc cgc ggc Lys Thr Xaa Giu Thr Giu Ile Arg Giy 170 tta act Leu Thr gca gtg nCt Ala Val Xaa ggc aac gaa Giy Asn Giu 195 cag acc agc atc Gin Thr Ser Ile tcc gtg cct tcc Ser Val Pro Ser atc cgt aag Ile Arg Lys 190 aac ctt cac Asn Leu His gca gct cac gcc Ala Ala His Ala atc Ile 200 ggc ctt ggc cag Giy Leu Gly Gin ggc tac Gly Tyr 210 ttc ggt cgc gag Phe Giy Arg Giu atg cac tac ggc Met His Tyr Giy gag gaa gcc ctg Giu Giu Ala Leu gac Asp 225 ttc acc aac gca Phe Thr Asn Ala ttt gct gcc gtg ctg tac cag tgc ctg Phe Ala Ala Vai Leu Tyr Gin Cys Leu 235 cgt Arg 240 gca tcc aac cag Ala Ser Asn Gin gct act gag cgt Ala Thr Giu Arg gga Giy 250 gag cgt ttc aag Giu Arg Phe Lys aac ttc Asn Phe 255 gaa aac tcc Glu Asn Ser aac gac ttc Asn Asp Phe 275 tat gca acc ggt Tyr Ala Thr Giy gag Giu 265 tac ttc gat gat Tyr Phe Asp Asp ttc gat gca Phe Asp Ala 270 ttt gcc aag.

Phe Ala Lys gca cca aag tcc Ala Pro Lys Ser gac Asp 280 aag gtc aag gaa Lys Val Lys Giu ctc Leu 285 tcg aac Ser Asn 290 atc cac acc cca Ile His Thr Pro gtt gag gac tgg Val Giu Asp Trp gcg ctg aag gcc Ala Leu Lys Aia 1466 WO 01/00843 WO 0100843PCT/EBOO/00923 gac gtg Asp Val 305 cca acc 1008 Pro Thr cca atc 1056 Pro Ile gtt tac 1104 Val Tyr gag gac 1152 Glu Asp 370 gt~g gca 1200 Val Ala 385 aag gac 1248 Lys Asp tgg cgc 1296 Trp Arg gct ctg 1341 Ala Leu atg gag cac Met Giu His ggt tcg atc Gly Ser Ile 325 gca tcc aag Ala Ser Lys ggt Giy 310 ctg ttc aac cgt Leu Phe Asn Arg aac Asn 315 tcC Ser ctg caa gcg gtt Leu Gin Ala Val cca 960 Pro 320 tcc tac atc aac aac Ser Tyr Ile Asn Asn 330 att gag atc cgc aag Ile Glu Ile Arg Lys 345 acc tcg tcg atc cac Thr Ser Ser Ile His 335 gaa ggc aag atc ggc cgc Glu Gly Lys Ile Gly Arg 350 tac Tyr 340 355 gcc Ala cca gct cca cac atg Pro Ala Pro His Met 360 tac gaa atc ggc tac Tyr Giu Ilie Gly Tyr gac aat gac aac ctt gag tac ttc Asp Asn Asp Asn Leu Giu Tyr Phe 365 gag aag atc att gac acc tac gct Giu Lys Ile Ile Asp Thr Tyr Ala acc aag tac gtt Thr Lys Tyr Val 390 act gcc acc acc Thr Ala Thr Thr gac cag ggc ctg tca Asp Gin Gly Leu Ser 395 cgt gac atc aac cgt Arg Asp Ilie Asn Arg 410 acc ttg ttc ttc Thr Leu Phe Phe 400 aag ggc Lys Gly 420 gaa ggc Giu Gly 405 atc aag acc ttg tac Ile Lys Thr Leu Tyr 425 act gaa gtt gac ggc Thr Giu Val Asp Gly 440 tac att Tyr Ile tgc gtc Cys Val gcg cag atc tac gca Ala Gin Ile Tyr Ala 415 cgc ctg cgc cag gtt Arg Leu Arg Gin Val 430 agc tgc atg ctg Ser Cys Met Leu 445 taaaagcact taaaaatatc ccc 1364 <210> 1070 <211> 447 <212> PRT <213> Corynebacterium glutamicum <400> 1070 Ser Phe Leu Asp Thr Lys Arg Giu Asn 1 5 Lys Thr Leu Ser Leu Gly Val Vai Ile 25 Ala Asp Giu Lys Ile Arg Ile 10 Pro Asp Ile Thr Phe Giu Leu 1467 WO 01/00843 Ala Lys Arg Arg Ile Tyr Asp Glu Met Arg Gin Phe Pro Tyr Ile Gly Arg Ile 115 *Thr Pro Ser 130 AspIle Ser 145 Ala Pro Asn Ala Val Xaa Gly Asn Giu 195 Gly Tyr Phe 210 Asp Phe Thr 225 Ala Ser Asn Glu Asn Ser Asn Asp Phe 275 Ser Asn Ilie 290 Asp Val Met 305 Pro Thr Gly Pro Ile Ala Asn Giy Val Phe Met 100 Thr Giu Cys Phe Giu 180 Ala Gly Asn Gin Lys 260 Ala His Gi u Ser Ser 340 Asp Lys Asp Gin Tyr His Phe Asn Giu 165 Gin Ala Arg Ala Ile 245 Tyr Pro Thr, His Ile 325 Lys Pro Ile Thr Phe Ser Ile Aia 140 Aia Ile Pro Gin Ser 220 Tyr Arg Asp Giu Aia 300 Leu Thr Giy Asp Giu Ile Ser Pro 110 Gin Vai Aia Giy Ile 190 Asn Glu Cys Lys Phe 270 Phe Leu Ala Ser Ile 350 PCTIiBOOIOO923 Vai Giu His Tyr Asfl Ala Gly Tyr Ile Glu Vai Ser Giy Giu Met Asp 160 Leu Thr 175 Arg Lys Leu His Ala Leu Leu Arg 240 Asn Phe 255 Asp Ala Ala Lys Lys Ala Vai Pro 320 Ile His 335 Giy Arg Vai Tyr Tyr Pro Ala Pro His Met Asp Asn Asp Asn Leu Giu Tyr Phe 1468 WO 01/00843 WO 0100843PCT/iBOO/00923 Giu Val 385 Lys Trp Ala 355 Ala Thr Thr Lys Giu 435 Tyr Giu Ile Gly 375 Lys Tyr Val Asp 390 Ala Thr Thr Arg 405 Gly Ile Lys Thr 420 Gly Thr Glu Val Giu Lys Ile Ile 380 Gly Leu Ser Leu 395 le Asn Arg Ala 410 Tyr Tyr Ile Arg 425 Gly Cys Val Ser 365 Asp Thr Gin Leu Cys 445 Thr Tyr Leu Phe Ile Tyr 415 Arg Gin 430 Met Leu Ala Phe 400 Ala Val <210> 1071 <211> 1125 <212> DNA <213> Corynebacteriun glutanicui <220> <221> CDS <222> (101)..(1102) <223> RXN01920 <400> 1071 cttctgaagg gcttcggttg gggtaagctg gcgatctgaa atcgcgctgc attgtggcgt cgaaaagcaa aaaaatttgt agaagggaag agcgcaccta atg gct gct gat tct Met Ala Ala Asp Ser ag t Ser atc Ile cgt Arg gac Asp atg Met gtc Val gcg Ala gtt Val aac Asn c tg Leu atc Ile cgc Arg ggc Gly gtg Val1 cac His tgg Trp acc Thr aag Lys gtg Val gca Ala cta Leu gc t Ala tcc Ser aac Asn tgg Trp acc Thr tcc Ser aac Asn gag Giu tcc Ser cca Pro aac Asn c tg Leu gac Asp atg Met 1 cat His aaa Lys gaa Glu gag Glu ctg Leu gca Ala gaa Glu gca Al a ctt Leu gtc Val gaa Glu acc Thr tca Ser gtg Val 115' 163 211 259 307 355 403 451 1469 WO 01/00843 PCT/IBOO/00923 aag agt Lys Ser aac gal Asn Asp 135 gca aag Ala Lys 150 aag atc Lys Ile tac ctc Tyr Leu gac gtg Asp Val att ggc Ile Gly 215 cag gaa Gin Glu 230 ga aac Asp Asn acc gag Thr Glu aac aac Asn Asn tcc cca 1027 Ser Pro 295 gac ttc 1075 Asp Phe 310 aac acc 1122 Asn Thr tac Tyr 120 gcg Ala atc Ile gcc Ala cca Pro atc Ile 200 tac Tyr gag Glu gaa Glu gat Asp ctt Leu 280 aac Asn ttc Phe gag Glu 105 tcc Ser ttc Phe atc Ile tcc Ser atg Met 185 cgc Arg aag Lys ctg Leu act Thr gtt Val 265 ggc Gly atc Ile tcC Ser gat Asp aac Asn cgt Arg ctg Leu gtg Va1 170 tat Tyr ctg Leu tat Tyr aag Lys cag Gin 250 aag Lys tac Tyr ttg Leu ggc Gly gat Asp 330 110 act ctg gcc Thr Leu Ala gaa aat gaa Glu Asn Glu gag ggc ga Glu Giy Asp 160 tcc ttc ctg Ser Phe Leu 175 cac tcc aag His Ser Lys 190 ga gag gca Asp Giu Ala gtc gcg aag Val Ala Lys ltc ga ctg Phe Asp Leu 240 gat ctc tac Asp Leu Tyr 255 cgc tac aac Arg Tyr Asn 270 ttc cca gcg Phe Pro Ala tca cca aac Ser Pro Asn tct tac gtt Ser Tyr Val 320 ttc taactttt Phe tcC Ser aac Asn 145 ga Asp ttc Phe ctg Leu gtg Val gag Glu 225 Ctc Leu gac Asp gcc Ala ga Asp gct Ala 305 att atc Ile aag Lys cgc Arg 165 ttc Phe gcc Ala tac Tyr cgt Arg tac Tyr 245 tgg Trp ctc Leu gtg Val cac His gaa 499 547 595 643 691 739 787 835 883 931 979 Ile Gly Lys Ala Glu 325 :aa aaagctgaag 1470 WO 01/00843 WO 0100843PCT/IBOO/00923 cgc 1125 <210> 1072 <211> 334 <212> PRT <213> Corynebacterium giutarnicum <400> 1072 Met Ala Ala Asp Ser Asp Leu Ser Val His Asp Ala Tyr Leu Lys Glu 1 His Lys Giu Glu Leu Ala Glu Ser Asn 145 Asp Phe Leu Val1 Giu 225 Leu Val Asp Lys Val1 Asp Asp Ser Thr 130 Leu Pro Tyr Ala His 210 Thr Tyr Lys Trp Ser Ala 70 Gly Giu Lys Asn Ala 150 Lys Tyr Asp Ile Gin 230 Asp Asn Leu Ile Arg Giy Vai 105 Ser Phe Ile Ser Met 185 Arg Lys Leu Thr Val 265 Trp Thr Lys Val Ala Leu Asn Arg Leu Val 170 Tyr Leu Tyr Lys Gln 250 Lys Pro Trp Thr Leu Leu Al a 110 Thr Giu Glu Ser His 190 Asp.

Val Phe Asp Arg 270 Asp Leu Leu Thr Pro Phe Leu Asn Gly Phe 175 Ser Giu Ala Asp Leu 255 Tyr Ser Pro Asn Leu Asp Met Aia Glu Asp 160 Leu Lys Ala Lys Leu 240 Tyr Asn Asp Asp Leu Trp Thr Glu Asp 1471 WO 01/00843 Ala Asn Lys Ala Leu 275 Asp Glu Thr Lys Val 290 Ala Asp Giu Asn His 305 Ilie Gly Lys Ala Glu 325 PCTJIBOO/00923 Asn Asn Leu Gly Tyr Glu Gly Leu Phe Pro Ala 280 285 Ser Pro Asn Ile Leu Ser Ala Leu Ser Pro Asn 295 300 Asp Phe Phe Ser Gly Ser Gly Ser Ser Tyr Val 310 315 320 Asn Thr Giu Asp Asp Asp Trp Asp Phe 330 <210> 1073 <211> 437 <212> DNA <213> Corynebacteriun giutanicun <220> <221> CDS <222> <223> FRXA01920 <400> 1073 gcc gac gtg atc Ala Asp Val Ile 1 tac att ggc tac Tyr Ile Gly Tyr cgt cag gaa gag Arg Gin Glu Giu tac gat aac gaa Tyr Asp Asn Glu tgg acc gag galt Trp Thr Giu Asp ctc aac aac ctt Leu Asn Asn Leu gtg tcc cca aac Val Ser Pro Asn 100 cac gac ttc ttc His Asp Phe Phe 115 ctg atc atc cgc Leu Ile Ilie Arg gat gag Asp Giu gca gtg cac ggc tac Ala Val His Gly Tyr 10 tat Tyr aag Lys cag Gin aag Lys 70 tac Tyr t tg Leu ggC Gly caa Gin gag Giu t ac Tyr 55 cga Arg gaa Giu tct Ser tcc Ser gcg aag gag Ala Lys Giu gat ctg ctc Asp Leu Leu ctc tac gac Leu Tyr Asp tac aac gcc Tyr Asn Ala 75 cca gcg gat Pro Ala Asp cca aac gct Pro Asn Ala tac gtt att Tyr Val Ile 125 act Thr tac Tyr gat Asp aac Asn gaa Giu gat Asp 110 ggt.

Gly cca gag Pro Giu gat ctt Asp Leu ctt gga Leu Gly aag gcc Lys Ala acc aag Thr Lys gag aac Glu Asn aag gca Lys Ala 48 96 144 192 240 288 336 384 gaa aac acc gag gat gat gac tgg gac ttc taacttttaa aaagctgaag 130 cgc Asp 135 Trp Asp Phe 1472 WO 01/00843 WO 0100843PCTAIBOOIOO923 <210> 1074 <211> 138 <212> PRT <213> Corynebacterium glutamicun <400> 1074 Ala Asp Val Ile Arg Leu Ile Ile Arg 1 5 Tyr Ile Gly Tyr Lys Tyr Gin Lys Ala Arg Gin Glu Glu Leu Lys Giu Tyr Thr 40 Tyr Asp Asn Giu Thr Gin Tyr Ser Glu 55 Trp Thr Glu Asp Val Lys Arg Phe Leu 70 Asp Val Phe Asp Arg Glu Ala Asp Leu Tyr Ala Lys Leu Tyr Asn Val Glu Leu Asp Ala Leu Asn Asn Leu Gly TIyr Giu Gly Leu Phe Pro Ala Asp Giu Thr Lys 90 Val Ser Pro Asn Ile Leu Ser Ala Leu Ser Pro Asn Ala Asp Glu Asn 100 105 110 His Asp Phe Phe Ser Gly Ser Gly Ser Ser Tyr Val Ile Gly Lys Ala 115 120 125 Glu Asn Thr Glu Asp Asp Asp Trp Asp Phe 130 135 <210> 1075 <211> 567 <212> DNA <213> Corynebacterium glutamicun <22 0> <221> CDS <222> (544) <223> RXA01080 <400> 1075 ttcagttctt cccttcaacg cccttgaagg gggaaactga taccagcaag cacactaggc ttgcgcacaa acggtattta gaagggaagt gagttcgagg atg cta atc gtg tat Met Leu Ile Val Tyr 1 ttt tcc tcg gcc acc gac aac acg cat cgt ttt gta caa aag ctc gat Phe Ser Ser Ala Thr Asp Asn Thr His Arg Phe Val Gin Lys Leu Asp 15 tta ccc aac gtg cgc atc ccc ctc act agg gtg gaa gaa ccg ctg aaa Leu Pro Asn Val Arg Ile Pro Leu Thr Arg Val Glu Glu Pro Leu Lys 30 1473 WO 01/00843 PCTJ/iBOO/00923 jia citc 259 atc aac gag ccc tac gtg cta atc acc Ile Asn Giu Pro Tyr Val Leu Ile Thr tcc atg act gga gaa aac tcc cgc ccg Ser Met Thr Giy Giu Asn Ser Arg Pro 60 ttt tta aat gat gaa cac aac cgc agc Phe Leu Asn Asp Glu His Asn Arg Ser 75 ggt gga aac tca aac ttc ggc tcc gat Giy Gly Asn Ser Asn Phe Gly Ser Asp att tcc aag aaa tgt aaa gtg ccc tat Ile Ser Lys Lys Cys Lys VIal Pro Tyr 105 110 ggc aat gag gaa gat gta agt atc ctt Gly Asn Giu Giu Asp Val Ser Ile Leu 120 125 gcc caa gct ttg ggg ctg gaa cca caa Ala Gin Ala Leu Gly Leu Giu Pro Gin 135 140 taaaccttaa aacttaatca atc <210> 1076 <2i1> 148 <212> PRT <213> Corynebacteriun giutamicun <400> 1076 Met Leu Ile Val Tyr Phe Ser Ser Ala 1 5 Val Gin Lys Leu Asp Leu Pro Asn Val 25 Giu Giu Pro Leu Lys Ile Asn Giu Pro 40 Tyr Gly Gly Gly Val Ser Met Thr Gly Pro Gin Val Ile Arg Phe Leu Asn Asp 70 Arg Ala Val Val Ala Gly Gly Asn Ser Leu Ala Gly Gu Ile Ile Ser Lys Lys 100 105 Arg Phe Giu Leu Met Gly Asn Giu Giu 115 120 acc tat Thr Tyr cca cca Pro Pro atc agg Ile Arg ggg ttg Gly Leu tac cgt Tyr Arg gga ggt Gly Gly cca gtt Pro Val 145 Thr Leu Ile Arg Arg Ser Pro Ile 125 His Thr Thr Pro Ser Asp Tyr 110 Leu Arg Arg Pro Val Phe Phe Val Arg Phe Val Thr Pro Ile Gly Tyr Gly 1474 WO 01/00843 WO 0100843PCTJIBOO/00923 Gly Leu Thr Gin Asn Ala Gin Ala Leu Gly Leu Giu Pro Gin Giu Pro 130 135 140 Val Thr Ser Arg 145 <210> 1077 <211> 650 <212> DNA <213> Corynebacteriun giutamicun <220> <221> <222> <223>

CDS

(627) RXA0 0867 <400> 1077 ttc ggc gac atg gac Phe Gly Asp Met Asp 1 5 ttc aag gtt gcc Phe Lys Val Ala acc gca gac ttc Thr Ala Asp Phe atc acc Ile Thr gca ctt cag Ala Leu Gin tcc gat gcg Ser Asp Ala gac acc aag ctg Asp Thr Lys Leu ggc att cct tcc Gly Ile Pro Ser aag gtg ctc Lys Val Leu atc ctg aac Ile Leu Asn ctt gag cag gca Leu Glu Gin Ala gat gcc cga ctg Asp Ala Arg Leu acc Thr acc atg Thr Met gct gat gtc atc aac gga cct gat gag Ala Asp Val Ile Asn Gly Pro Asp Giu agc aag ttc gct Ser Lys Phe Ala cc t Pro cgc atc acc acc Arg Ile Thr Thr gtg Val aag atc cca gtg Lys Ile Pro Vai aag atc ggt gag Lys Ile Gly Giu atc gga cca aag Ile Gly Pro Lys aag aac atc aac Lys Asn Ile Asn ctt acc gaa gag Leu Thr Glu Glu acc ggc Thr Giy gca aac atc Ala Asn Ile atc gaa gat gac Ile Giu Asp Asp acc gtg ttc atc Thr Val Phe Ile tct gca gct Ser Ala Aia 110 gct ctg gcg Ala Leu Ala gac ggc gca tct gct gaa gcg Asp Gly Ala Ser Ala Giu Ala 115 gcg Ala 120 atc gaa aag atc Ile Glu LysIle aac cca Asn Pro 130 cag ctg cca aag Gin Leu Pro Lys ggc gag cgc ttc ctc gga acc gtc gtc Gly Glu Arg Phe Leu Gly Thr Val Val 432 480 aag Lys 145 acc acc gca ttc Thr Thr Ala Phe gga Gly 150 gca ttc gtt tcc ttg ctc cca ggc cgc Ala Phe Val Ser Leu Leu Pro Gly Arg 155 gac Asp 160 ggc ctt gtt cac Gly Leu Val His tcc aag ctg ggt Ser Lys Leu Gly aac Asn 170 ggc aag cga gta Gly Lys Arg Val gaa aag Glu Lys 175 1475 WO 01/00843 WO 0100843PCTfIiBOO/00923 gtc gac gat gtg gtg aag gtt ggc.gag aag att cag gtc gaa atc gct 576 Val Asp Asp Val Val Lys Val Gly Giu Lys Ile Gin Val Giu Ile Ala 180 185 190 gac atc gac aac cgc ggc aag atc tcc ttg gtc cca gtt gtt gaa gag 624 Asp Ile Asp Asn Arg Gly Lys Ile Ser Leu Val Pro Vai Vai Giu Giu 195 200 205 gac taattagttc tggctagatc ggg 650 Asp <210> i078 <2ii> 209 <212> PRT <213> Corynebacteriun giutamicum <400> 1078 Phe Gly Asp Met Asp Phe Lys Vai Ala Gly Thr Aia Asp Phe Ile Thr 1 5 10 Ala Leu Gin Leu Asp Thr Lys Leu Asp Gly Ilie Pro Ser Lys Vai Leu 25 Ser Asp Ala Leu Glu Gin Ala Arg Asp Ala Arg Leu Thr Ile Leu Asn 40 Thr Met Ala Asp Val Ilie Asn Gly Pro Asp Glu Met Ser Lys Phe Ala 55 Pro Arg Ile Thr Thr Val Lys Ile Pro Val Ala Lys Ile Gly Glu Leu 70 75 Ile Gly Pro Lys Gly Lys Asn Ilie Asn Ala Leu Thr Glu Giu Thr Gly -90 Ala Asn Ile Ser Ilie Giu Asp Asp Gly Thr Val Phe Ile Ser Ala Ala 100 105 110 Asp Gly Ala Ser Ala Giu Ala Ala Ile Glu Lys Ilie Asn Ala Leu Ala 115 120 125 Asn Pro Gin Leu Pro Lys Val Gly Giu Arg Phe Leu Gly Thr Val Val 130 135 -140 Lys Thr Thr Ala Phe Gly Ala Phe Val Ser Leu Leu Pro Gly Arg Asp 145 150 155 160 Gly Leu Val His Ile Ser Lys Leu Gly Asn Gly Lys Arg Val Glu Lys 165 170 175 Val Asp Asp Val Val Lys Val Gly Giu Lys Ile Gin Val Glu Ile Ala 180 185 190 Asp Ile Asp Asn Arg Gly Lys Ile Ser Leu Val Pro Val Val Glu Glu 195 200 205 Asp 1476 WO 01/00843 WO 0100843PCT/IBOO/00923 <210> 1079 <211> 630 <212> DNA <213> Corynebacteriun glutanicun <220> <221> <222>

CDS

<223> RXA01416 <400> 1079 gct ggc gct tcc Ala Gly Ala Ser 1 gaa Glu 5 aac gtt gtc aac Asn Val Val Asn gtc aag gac ggt Val Lys Asp Gly gca cca Ala Pro gca cca acc Ala Pro Thr ttc atc gac Phe Ile Asp aag at~c gtc tcc Lys Ile Val Ser ggc ctt gaa gca Gly Leu Glu Ala gct aag cca Ala Lys Pro cag cgc gtt Gln Arg Val atc ctg tgc cgc Ile Leu Cys Arg cag gaa ggt ctg Gln Glu Gly Leu gga aac Gly Asn gca gcc aag gaa Ala Ala Lys Glu cca ctg ttc cct Pro Leu Phe Pro tac acc gac gag Tyr Thr Asp Glu gtg Val tac tcc gca gtg Tyr Ser Ala Val cgc aag gta tcc Arg Lys Val Ser aag cta gct tct Lys Leu Ala Ser ctg acc ctg aag Leu Thr Leu Lys gca Ala aag caa gag cgc Lys Gln Glu Arg gac gac gct acc aac Asp Asp Ala Thr Asn gcc tac Ala Tyr atg gaa gaa met Glu Glu agc tca gca Ser Ser Ala 115 gaa gcc gaa ctg ctt cca aag ttc gag Glu Ala Glu Leu Leu Pro Lys Phe Glu 105 gct tcc tac Ala Ser Tyr 110 tac aac gct Tyr Asn Ala gct gaa gcg tcc Ala Glu Ala Ser gaa atc cgt gca Glu Ile Arg Ala gtc atg Val Met 130 aag gcc atc gtg Lys Ala Ile Val cgc cgc atg atc ctc acc gat cac ttc cgc Arg Arg Met Ile Leu Thr Asp His Phe Arg gac ggc cgc gga gtc acc gac atc cgt Asp Gly Arg Gly Val Thr Asp Ile Arg 150 ctg gca gta gaa Leu Ala Val Glu gag ctc atc cca Glu Leu Ile Pro gcg cac ggt tcc Ala His Gly Ser ctc ttc gag cgt Leu Phe Glu Arg ggc gag Gly Glu 175 528 576 acc cag atc Thr Gln Ile ggt gtc acc acc ctg gac atg ctc aag Gly Val Thr Thr Leu Asp Met Leu Lys 185 atg gaa cag Met Glu Gln 190 1477 WO 01/00843 PCTIIBOO/00923 caa atc gac tcc Ctg gca cca ggc gat gcg aag cgc tac atg cac cac 624 Gin Ile Asp Ser Leu Ala Pro Gly Asp Ala Lys Arg Tyr Met His His 195 200 205 tac aac 630 Tyr Asn 210 <210> 1080 <211> 210 <212> PRT <213> Corynebacterium giutamicum <400> 1080 Ala Gly Ala Ser Glu Asn Val Val Asn Arg Vai Lys Asp Gly Ala Pro 1 5 10 Ala Pro Thr Giu Lys Ilie Val Ser Asp Gly Leu Giu Ala Ala Lys Pro 25 Phe Ile Asp Ile Leu Cys Arg Ala Gin Giu Gly Leu Ala Gin Arg Val 40 Gly Asn Ala Ala Lys Glu Phe Pro Leu Phe Pro Pro Tyr Thr Asp Glu 55 Val Tyr Ser Ala Val Giu Arg Lys Val Ser Lys Lys Leu Ala Ser Leu 70 75 Leu Thr Leu Lys Ala Lys Gin Giu Arg Asp Asp Ala Thr Asn Ala Tyr 90 Met Giu Giu Ile Giu Ala Giu Leu Leu Pro Lys Phe Giu Ala Ser Tyr 100 105 110 Ser Ser Ala Ala Giu Ala Ser Lys Giu Ile Arg Ala Gly Tyr Asn Ala 115 120 125 Val Met Lys Ala Ile Val Arg Arg Met Ile Leu Thr Asp His Phe Arg 130 135 140 Ile Asp Gly Arg Gly Val Thr Asp Ile Arg Asp Leu Ala Val Giu Vai 145 150 155 160 Glu Leu Ile Pro Arg Ala His Gly Ser Ser Leu Phe Giu Arg Gly Giu 165 170 175 Thr Gin Ile Leu Gly Vai Thr Thr Leu Asp Met Leu Lys Met Glu Gin 180 185 190 Gin Ile Asp Ser Leu Ala Pro Giy Asp Ala Lys Arg Tyr Met His His 195 200 205 Tyr Asn 210 <210> 1081 <211> 757 <212> DNA 1478 WO 01/00843 <213> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(757) <223> R.XA01486 <400> 1081 agagaactgg taaggttttt accgttctag accgcagaaa PCTIBOO/00923 tcttcgcggc .gacaccgatg atcgccgagc agaactaaac atgaggagac ctactcgcat atg agc gat gta aag Met Ser Asp Val Lys ttc Phe aac Asn gcc Ala atg Met gac Asp ggt Gly gaa Giu ttt Phe tcc Ser 135 tcc Ser ggc Gly tgg Trp, gac Asp gac Asp cag Gin c tg Leu ttC Phe atc Ile atc Ile 105 aag Lys aac Asn gca Ala cgc Arg gca Ala 185 ggC Gly cgc Arg gca Ala acc Thr gtg Val1 t tc Phe cgt Arg aat Asn 125 tac tcc Ser gtt Val c ac His ctg Leu acc Thr 30 gtg Val1 gca Ala gac Asp ttc Phe ctc Leu 110 gag Giu tac Tyr gga Gly ggt Gly gCt Ala 190 atc Ile atc Ile acc Thr tcc Ser gtt Val1 cgt Arg atc Ile gtt Val gat Asp ctt Leu ga t Asp 175 caa Gin gag Giu cgt Arg acc Thr aac Asn gaa Glu cgg Arg gac Asp cag Gin gtc Val1 cct Pro 160 gaa Giu cat His 1 gcc Aia ttt Phe tac Tyr cag Gin gag Giu gag Giu cgc Arg atc Ile gta Val1 145 gtc Val aag Lys gag Glu gtc Val gaa Giu ctc Leu c ca Pro cgt Arg ggt Gly cca Pro gtt Val1 130 gca Ala tc C Ser cac His cag Gin gca Ala ac C Thr gac Asp cgc Arg atg Met cgc Arg ctg Leu 115 gtc Val atc Ile Gly cca Pro tcC Ser 195 1479 WO 01/00843 WO 0100843PCT/IBOO/00923 gaa atc gtt gtg gct ggt cgc ctc gtc gag cgc aag cgc ggc aac aag 739 Giu Ile Val Val Ala Gly Arg Leu Val Giu Arg Lys Arg Gly Asn Lys 200 205 210 acc ttc tcc gac gtc gca 757 Thr Phe Ser Asp Val Ala 215 <210> 1082 <211> 219 <212> PRT <213> Corynebacteriun glutamicum <400> 1082 Met Ser Asp Val Lys Asp Phe Glu Asp Thr Giu Phe Gly Leu Ile Giu 1 5 10 Ala Val Ala Thr Ile Asp Asn. Gly Asp Phe Gly Thr Arg Thr Ile Arg 25 Phe Glu Thr Gly Gin Leu Ala Arg Gin Ala Asp Gly Ala Val Thr Thr 40 Tyr Leu Asp Asp Asp Thr Met Leu Leu Ala Thr Thr Thr Ala Ser Asn 55 Gin Pro Arg Glu Gly Phe Asp Phe Phe Pro Leu Thr Vai Asp Val Glu 70 75 Glu Arg Met Tyr Ala Ala Gly Arg le Pro Gly.Ser Phe Phe Arg Arg 90 Giu Gly Arg Pro Ser Thr Glu Ala Ile Leu Ala Cys Arg Leu Ile Asp 100 105 110 Arg Pro Leu Arg Pro Thr Phe Val Lys Gly Leu Arg Asn Giu Val Gin 115 120 125 Ile Val Val Thr Val Met Ser Met Asn Pro Giu Asp Tyr Tyr Asp Val 130 135 140 Val Ala Ile Asn Gly Ala Ser Ala Ala Thr Arg Ile Ser Gly Leu Pro 145 150 155 160 Val -Ser Gly Ala Val Gly Gly Val Arg Met Ala Leu Val Gly Asp Giu 165 170 175 Lys His Pro Giu Gly Gin Trp Val Ala Phe Pro Thr His Ala Gin His 180 185 190 Glu Gin Ser Val Phe Glu Ile Val Val Ala Gly Arg Leu Val Glu Arg 195 200 205 Lys Arg Gly Asn Lys Thr Phe Ser Asp Val Ala 210 215 <210> 1083 <211> 651 1480 WO 01/00843 WO 0100843PCTIIBOO/00923 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS <222> (628) <223> RXA01678 tattgtccag gcgcaggaaa atatctcata gttcaacatc cggctatatc tctgcaactg cagctcaccc cggtgcagca gcaaatatca ccgacttcca atg ctg aaa tgt gca 11~ Met Leu Lys Cys Ala 163 gtc gat gaa gcc gct ggc gga cgc gcc Val Asp Giu Ala Ala Gly Gly Arg Ala gct ttc gta tcc Ala Phe Val Ser tca ggc Ser Gly gat aac att Asp Asn Ile ccc acc ttg Pro Thr Leu ggc agc ccg ttc Gly Ser Pro Phe tcc tcc att ctt Ser Ser Ile Leu ggt gat gaa Gly Asp Glu tca gca gtg Ser Ala Val gaa gca ctc aac Giu Ala Leu Asn atg ggt ctt gat Met Gly Leu Asp ggc aac Gly Asn cac gaa ttt gat His Giu Phe Asp ggc tac gca gac Gly Tyr Ala Asp agc agt cga gtc Ser Ser Arg Val gct Ala gac ctt gct gat Asp Leu Ala Asp gat tat ctc ggc gca aac gtt gag ggc Asp Tyr Leu Gly Ala Asn Val Giu Gly 80 gaa Glu 355 aac cca gat ctt Asn Pro Asp Leu cca tat gga att Pro Tyr Gly Ile cac ctt gat ggt His Leu Asp Gly gtg aag Val Lys 100 gtt gct ttc Val Ala Phe tct gaa ggc Ser Glu Gly 120 gta Val 105 ggc acc gta tcc Gly Thr Val Ser c aa Gin 110 gaa act ccg atg Glu Thr Pro Met ttg gtc aat Leu Val Asn 115 gaa gca acc Glu Ala Thr att gag gga atc Ilie Glu Giy Ile ttt act gac cca Phe Thr Asp Pro aac cgt Asn Arg 135 gta gct gat gaa Val Ala Asp Glu gtg gga agt ggc Val Gly Ser Gly gca Ala 145 gca gat gtt gtc Ala Asp Val Val gcg ctt tac cac Ala Leu Tyr His ggc att acc ggc Gly Ile Thr Gly acc Thr 160 gaa gca tgg tca Giu Ala Trp Ser aat atc gac gtt Asn Ile Asp Val gtt Val 170 ttc gca ggt cac Phe Ala Gly His acc cac Thr His 175 taagttcgtg atctaggaac 648 WO 01/00843 WO 0100843PCT/IBOO/00923 <210> 1084 <211> 176 <212> PRT <213> Corynebacteriun giutamicun <400> 1084 Met Leu Lys Cys Ala Val Asp Glu Ala Ser Gly Ser Ser Glu Gly Leu 115 Glu Asp Ser Asp Ala Arg Giy Val1 100 Val Ala Val1 Gly Asp Giu Pro Val Gly Val Ala Giu Asn Lys Val Asn Ser Thr Asn Val Val 150 Gly Pro Asn Lys Asp Tyr Val1 Ile Leu 140 Gly Arg Phe Gin Gly Tyr Gly Ser Thr 125 Val1 Ile Ala Gin Met Tyr Leu Ile Gin 110 Phe Gly Thr Glu Ala Trp Ser Giu Asn Ile Asp Val Val Phe Ala Gly His Thr His 165 170 175 <210> 1085 <211> 1359 <212> DNA <213> Corynebacterium glutanicun <220> <221> CDS <222> (101)..(133,6) <223> RXA01679 <400> 1085 cattaccggc accgaagcat ggtcagaaaa tatcgacgtt gttttcgcag gtcacaccca ctaagttcgt gatctaggaa ccgacaacgg tccactaatc atg cag tct gga aac 115 Met Gin Ser Gly Asn 1 1482 WO 01/00843 tac ggg cac Tyr Gly His ggt gag ctc Gly Glu Leu aac gcg tgt Asn Ala Cys gcg gaa ctt Ala Glu Leu gat ggc gat Asp Gly Asp aac tac ggc Asn Tyr Gly cgt tgg tcc Arg Trp Ser atg aac gcc Met Asn Ala 120 tac gcc gaa Tyr Ala Glu 135 gtc acc ctc Val Thr Leu 150 gaa gaa ggt Glu Glu Gly gtt tcc tac Val Ser Tyr tcc gtg acc Ser Val Thr 200 gtt gca gct Val Ala Ala 215 ctg acc cgc Leu Thr Arg 230 gca Ala acc Thr 25 gaa Glu gat Asp t tt Phe gct Ala atg Met 105 gga Gly gct Ala aag Lys tct Ser acc Thr 185 att Ile tcc Ser gga Gly ctt Leu 10 gta Val1 aac Asn gc t Ala ctc Leu gaa Glu tcc Ser gga Gly t tt Phe gga Gly gca Ala 170 tac Tyr gat Asp c tg Leu ac c Thr gta gat Val Asp gcc cgc Ala Arg gac acc Asp Thr 45 gcc ggc Ala Gly agc gac Ser Asp ctc gtc Leu Val acc gcc Thr Ala 110 act gac Thr Asp 125 cag cct Gin Pro ttc aaa Phe Lys gtg gca Val Ala aac cgt Asn Arg 190 cca ctt Pro Leu 205 cag tcc Gin Ser gca caa Ala Gin ttc agc Phe Ser 15 atg ctc Met Leu att gca Ile Ala aaa gaa Lys Giu gaa gga Glu Gly 80 aac atg Asn Met 95 acc acc Thr Thr cta -ttc Leu Phe ttc tcc Phe Ser gat gcc Asp Ala 160 gca ctt Ala Leu 175 cca atc Pro Ile gat ccg Asp Pro ggc aac Gly Asn acc ggc Thr Gly 240 ttc Phe gga Gly gat Asp gta Val1 gca Ala att Ile gca Ala agc Ser ggt Gly 145 ct t Leu ggC Gly ggt Gly gaa Glu gaa Giu 225 atc Ile aac Asn gtc Val1 att Ile gta Val1 gaa Giu gcc Ala gac Asp ggC Gly.

130 gaa Glu gac Asp gta Val gac Asp cgc Arg 210 ggt Gly gtg Val1 PCT/iBOO/00923 gac acc 163 Asp Thr gat atc 211 Asp Ile gct cag 259 Ala Gin acc atc 307 Thr Ile ggc tcc 355 Gly Ser gct gtg 403 Ala Val 100 ggg ctt 451 Gly Leu gtt acc 499 Val Thr tca ttt 547 Ser Phe cag tgg 595 Gin Trp 165 gac aac 643 Asp Asn 180 gtc act 691 Val Thr tac gtt 739 Tyr Val acc gca 787 Thr Ala gta cag 835 Val Gin 245 tcc acc atc gga tac ttg tcc aac aac aat gtc acc cca cgt act ggt 1483 WO 01/00843 PCTIIBOO/00923 Arg Thr Gly 260 Ser Thr caa gcc Gin Ala acc atc Thr Ilie gcc acg 1027 Ala Thr 295 att gat 1075 Ilie Asp 310 aca gtg 1123 Thr Val gtt gtc 1171 Val Val gtt ggc 1219 Val Gly gga act 1267 Giy Thr 375 gga atg 1315 Giy Met 390 atc cag 1359 Ile Gin Ile Giy Tyr 250 cag att tcc Gin Ile Ser 265 acc ctt gac Thr Leu Asp 280 gaa gta act Giu Vai Thr cct cag ctc Pro Gin Leu agc ttg gac Ser Leu Asp 330 acc acc gat Thr Thr Asp 345 gca gaa caa Ala Giu Gin 360 gga gtg ctc Giy Vai Leu gtc ggt ctg Vai Gly Leu cag caa atc Gin Gin Ilie 410 Leu Ser Asn Asn Asn Vai Thr Pro 255 atc acc Ile Thr atg gca Met Ala gtc agc Val Ser 300 gga gaa Gly Giu 315 att cct Ilie Pro acc ggc Thr Giy cca gca Pro Ala agt gga Ser Giy 380 gtg aac Val Asn 395 ttt gct Phe Ala tcc ggt Ser Gly 270 ctc cgc Leu Arg cga gaa Arg Giu ggc ttt Gly Phe acc ctt Thr Leu 335 cgt att Arg Ile 350 caa cca Gin Pro ctc ggc Leu Gly att gac ttc Phe acc Thr att Ile 305 gaa Giu gg t Gly atg met ggt Gly gtt Val1 385 agc aat Asn caa Gin 290 gtt Val gct Ala act Thr cca Pro t cc Ser 370 gta Val ttc gaa Giu act Thr cca Pro gcg Ala 325 ctc Leu att Ile tta Leu c ta Leu cag Trp Ile Asp Pro Ser Phe Ile Gin Gin taaatttagt aaaaagcatg cat <210> 1086 <211> 412 <212> PRT <213> Corynebacterium giutamicum <400> 1086 Met Gin Ser Gly Asn Tyr Gly His Ala Leu Ala Asp Val Asp Phe Ser 1 5 10 1484 WO 01/00843 PCT/IB00/00923 Phe Asn His Asp Thr Gly Glu Leu Thr Val Asp Asp Ala Arg Met Leu 25 Gly Val Asp Asp Ile Asn Ala Cys Glu Asn Pro Asp Asp Thr Ile Ala 40 Asp Ile Val Ala Gin Ala Glu Leu Asp Ala Gly Glu Ala Gly Lys Glu 55 Val Val Ala Thr Ile Asp Gly Asp Phe Leu Arg Ala Ser Asp Glu Gly 70 75 Ala Glu Ser Gly Ser Asn Tyr Gly Ala Glu Ser Gin Leu Val Asn Met 90 Ile Ala Ser Ala Val Arg Trp Ser Met Ser Thr Asn Thr Ala Thr Thr 100 105 110 Ala Asp Ile Gly Leu Met Asn Ala Gly Gly Leu His Thr Asp Leu Phe 115 120 125 Ser Gly Asp Val Thr Tyr Ala Glu Ala Phe Glu Ile Gin Pro Phe Ser 130 135 140 Gly Glu Asp Ser Phe Val Thr Leu Lys Gly Ser Val Phe Lys Asp Ala 145 150 155 160 Leu Asp Gin Gin Trp Glu Glu Gly Ser Ala Arg Pro Val Ala Ala Leu 165 170 175 Gly Val Ser Asp Asn Val Ser Tyr Thr Tyr Asp Ile Asn Arg Pro Ile 180 185 190 Gly Asp Arg Val Thr Ser Val Thr Ile Asp Asp Thr Pro Leu Asp Pro 195 200 205 Glu Arg Asp Tyr Val Val Ala Ala Ser Leu Tyr Leu Gin Ser Gly Asn 210 215 220 Glu Gly Met Thr Ala Leu Thr Arg Gly Thr Ala Pro Ala Gin Thr Gly 225 230 235 240 Ile Val Asp Val Gin Ser Thr Ile Gly Tyr Leu Ser Asn Asn Asn Val 245 250 255 Thr Pro Arg Thr Gly Gin Ala Gin Ile Ser Ile Thr Pro Ser Gly Glu 260 265 270 Phe Asn Ala Gly Glu Thr Ile Thr Leu Asp Met Ala Gly Leu Arg Tyr 275 280 285 Thr Gin Gly Asp Thr Ala Thr Glu Val Thr Val Ser Leu Arg Glu Glu 290 295 300 Ile Val Ser Ala Pro Ile Asp Pro Gin Leu Gly Glu Ala Gly Phe Gly 305 310 315 320 Glu Ala Gly Thr Ala Thr Val Ser Leu Asp Ile Pro Ala Thr Leu Ser 325 330 335 Gly Thr Gin Asn Leu Val Val Thr Thr Asp Thr Gly Thr Arg Ile Ser 1485 WO 01/00843 WO 0100843PCTIiBOOIOO923 340 345 350 Met Pro Val Giu Ile Val Gly Ala Giu Gin Pro Ala Pro Gin Pro Ala 355 360 365 Gly Ser Ser Val Leu Giy Thr Gly Val Leu Ser Gly Leu Leu Gly Ile 370 375 380 Val Val Gly Ile Leu Gly Met Val Gly Leu Vai Asn Trp Ile Asp Pro 385 390 395 400 Ser Phe Ile Gin Gin Ile Gin Gin Gin Ile Phe Aia 405 410 1087 <211> 1071 <212> DNA <2i3> Corynebacterium giutaiicun <22 0> <221> CDS <222> (101)..(1048) <223> RXN01488 <400> 1087 ccagcgctta aggccggtca ccggccatca agcttgtcac atcgggtgcc ttatgatggt gcccgacctt aaaaataaaa acctgaaagg ttaaaaacgc atg agc aaa aaa gcc atc Ile gca Aia gg t Gly ga t Asp gca Ala c ac His aag Lys ctt Leu ctg Leu aac Asn ctg Leu cag Gin gga Gly gca Ala ga t Asp ggc Giy gtg Vai ttc Phe atc Ile tca Ser c ta Leu ggt Gly acc Thr gaa Giu gaa Giu cca Pro ggc Gly 75 atc Ilie gca Ala ggC Gly c ta Leu acc Thr gaa Giu 60 ttt Phe ggC Gly gtg Val gcc Ala gg t Gly aa t Asn tac Tyr gag Giu ctg Leu att Ile met 1 ctc Leu gtc Val1 gac Asp ttg Leu atc Ile cca Pro gat Asp Ser gca Ala acc Thr c tg Leu ggt Gly tc Ser gca Ala tCC Ser acc aag gat Thr Lys Asp caa Gin ctc Leu aac Asn

CCC

Pro 105 cac ggc gat gac ctg gtg atc atc gca act ggt ccc atg acc aac Ctg 499 1486 WO 01/00843 PCTII1BOO/00923 His Gly Asp Asp Leu Val Ile Ile Ala Thr Gly Pro Met Thr Asn Leu 120 125 130 tct gcg gca atc gca aag gat cca agc ttt gct tcc aag gct cac gtg 547 Ser Ala Ala Ile Ala Lys Asp Pro Ser Phe Ala Ser Lys Ala His Vai 135 140 145 gtc atc atg ggt ggc gcc ttg act gtc cca ggc aac gtc agc aca tgg 595 Val Ile Met Gly Gly Ala Leu Thr Val Pro Giy Asn Val Ser Thr Trp 150 155 160 165 gca gaa gca aac atc aac cag gac cca gat gca gca aac gat ctg ttc 643 Ala Glu Ala Asn Ile Asn Gin Asp Pro Asp Ala Ala Asn Asp Leu Phe 170 175 180 cgt tcc ggt gca gat gtc acc atg atc ggt ctt gat gtc acc ctg cag 691 Arg Ser Gly Ala Asp Val Thr Met Ile Gly Leu Asp Val Thr Leu Gin 185 190 195 accctt ctt acc aag aag cac act gcg cag tgg cgc gaa ctg ggc act 739 Thr Leu Leu Thr Lys Lys His Thr Ala Gin Trp, Arg Giu Leu Gly Thr 200 205 210 cca gct gct atc gca ctg gcc gac atg act gat tac tac atc aag gca 787 Pro Ala Ala Ile Ala Leu Ala Asp Met Thr Asp Tyr Tyr Ile Lys Ala 215 220 225 tat gag acc acc gca cca cac ctg ggc ggt tgc ggc ctg cac gac cca 835 Tyr Giu Thr Thr Ala Pro His Leu Gly Gly Cys Gly Leu His Asp Pro 230 235 240 245 ctg gca gta ggc gtt gca gtg gac cca agc ctg gtc actttg ctc ccc 883 Leu Ala Vai Giy Val Ala Vai Asp Pro Ser Leu Val Thr Leu Leu Pro 250 255 260 atc aac ctc aaggta gac att gag ggc gag acc cgt gga cgc acc att 931 Ile Asn Leu Lys Val Asp Ile Giu Gly Giu Thr Arg Giy Arg Thr Ile 265 270 275 ggc gat gaa gtc cgc ctc aac gat cca gtg cgc acc tcc cgc gca gct 979 Gly Asp Giu Val Arg Leu Asn Asp Pro Val Arg Thr Ser Arg Ala Ala 280 285 290 gtc gcc gta gac gtg gat cgt ttc ctt tct gaa ttc atg acc cgc atc 1027 Val Ala Val Asp Val Asp Arg Phe Leu Ser Giu Phe Met Thr Arg Ile 295 300 305 ggc cga gtc gca gca cag cag taaaagcagc tctggtgaag gtt 1071 Gly Arg Vai Ala Ala Gin Gin 310 315 <210> 1088 <211> 316 <212> PRT <213> Corynebacteriun giutamicum <400> 1088 Met Ser Lys Lys Ala Ile Leu Asp Ile Asp Thr Gly Ile Asp Asp Ala 1487 WO 01/00843 Leu Val Asp Leu Ile Pro Asp Pro Ser 145 Asn Ala Asp Arg Tyr 225 Gly Val Arg Thr Phe 305 Ala Thr Leu Gly Ser Ala Ser Met 130 Lys Val1 Asn Val Giu 210 Tyr Leu Thr Gly Ser 290 Met Tyr Tyr Leu His Ile Ser Thr Leu Val Trp 165 Phe Gin Thr Ala Pro 245 Pro Ile Ala Aia Gly Asp Al a 70 His Lys His Ser Val1 150 Ala Arg Thr Pro Tyr 230 Leu Ile Gly Val Leu Asn Leu 55 Gin Gly Ala Giy Ala 135 Ile Glu Ser Leu Ala 215 Glu Al a Asn Asp Ala 295 Gly Val Phe Thr Gin Leu Asp 120 Ala Met Ala Gly Leu 200 Ala Thr Val1 Leu Glu 280 Val Ser Leu Gly Lys Asn Pro 105 Asp Ile Gly Asn Ala 185 Thr Ile Thr Gly Lys 265 Val Asp Giu Glu Pro Gly 75 Ile Ala Val Lys Ala 155 Asn Val Lys Leu Pro 235 Ala Asp Leu Asp Leu Thr Glu Phe Gly Val Ile Asp 140 Leu Gin Thr His Ala 220 His Val Ile Asn Arg 300 Leu Ala Pro Val1 Val1 Phe 110 Ala S er Val1 Pro Ile 190 Ala Met Gly Pro Giy 270 Pro Leu PCTJIBOO/00923 Ile Gly Val Asn Val Tyr Leu Giu Giu Leu Leu Ile Thr Gly Phe Ala Pro Gly 160 Asp Ala 175 Gly Leu Gin Trp Thr Asp Gly Cys 240 Ser Leu 255 Giu Thr Vai Arg Ser Glu Arg Ile Gly 310 Arg Val Ala Ala Gin Gin 315 <210> 1089 1488 WO 01/00843 <211> 366 <212> DNA <213> Corynebacterium giutanicun <220> <221> CDS <222> (101)..(343) <223> RXCO0540 <400> 1089 tctccctatc taagggctgg ctaggtttga ccagtggcaa aaacattgtc ctccccattt cttgagtaag ggaaaatacc PCTIBOO/00923 gtc aat gtc atg cct aag gct gag att ctg Val Asn Val Met Pro'Lys Ala Giu Ile Leu 15 gta cac cgc gcc ctc gga cgt atc gga gtt Val His Arg Ala Leu Gly Arg Ile Gly Vai 30 cgt cag gga aag cgc ttc gag ctt gag gta Arg Gin Gly Lys Arg Phe Giu Leu Giu Val 45 gct gac cta aag aaa att gct gaa acc ctc Ala Asp Leu Lys Lys Ile Ala Giu Thr Leu 60 gaa gac ttc gat gtg gtg gga gtt gag gtc Giu Asp Phe Asp Val Val Gly Val Giu Val 75 aatcggtgtc att <210> 1090 <211> 81 <212> PRT <213> Corynebacterium giutamicun <400> 1090 Val Ala Arg Val Vai Val Asn Val Met Pro 1 5 10 Pro Gin Gly Gin Ala Val His Arg Ala Leu 25 Gly Val Ser Asp Val Arg Gin Gly Lys Arg 40 Asp Ser Val Thr Glu Ala Asp Leu Lys Lys 55 Ala Asn Thr Val Ile Giu Asp Phe Asp Val 70 Lys gat Asp tct Ser gat Asp ctc Leu gcg Ala Lys Gly Phe Ile Val 75 cagttacact tgtgctcgta gtg gcc cgt gta gtt Val Ala Arg Val Val 1 ccc cag ggg cag gcg Pro Gin Gly Gin Ala ggc gtt tcc gat gtc Gly Val Ser Asp Val gat tcc gtc acc gaa Asp Ser Val Thr Giu gca aac acc gtc atc Ala Asn Thr Val Ile aag tgagcgccaa Lys Ala Giu Ile Leu Asp Arg Ile Gly Val Ser Giu Leu Giu Val Asp Ala Glu Thr Leu Leu Gly Val Glu Val Ala 115 163 211 259 307 353 1489 WO 01/00843 WO 0100843PCTIBOOIOO923 <210> 1091 <211> 498 <212> DNA <213> Corynebacterium giutamicum <220> <221> <222> <223>

CDS

(101)..(475) RXCO0560 <400> 1091 aaggacgact tctggggagc ggtgtggatt ctcacgggga ttctcatgcg gattatcaga catatggaca ctttaacggt tcgtactagg ctgatgcttc atg agg att gat Met Arg Ile Asp ctg gaa acc cgg caa gcc gta ttg gcc Leu Giu Thr Arg Gin Ala Val Leu Ala aaa gac tgg att Lys Asp Trp Ile gaa ggg Giu Gly gag gga gac Giu Gly Asp cgc ctg agc Arg Leu Ser aaa aag cct ggt Lys Lys Pro Giy gcg gca ctt gcc Ala Ala Leu Ala gcc gca act Ala Ala Thr aac agc gtg Asn Ser Val gtc cga ctg ctc gcg caa cac gcg Val Arg Leu Leu Ala Gin His Ala 45 ccg gga Pro- Gly gag gtg Giu Val cgg gta ccc cca Arg Val Pro Pro gtt gcg gtg caa tgc ata gag ggg cca Val Ala Val Gin Cys Ile Giu Gly Pro cat aca cgc ggc His Thr Arg Gly cca ccc aac gtg Pro Pro Asn Val gag acc gac gcc Giu Thr Asp Ala acc tgg tta cgc Thr Trp Leu Arg gca cct ggg caa Ala Pro Gly Gin aca ttt gat gca Thr Phe Asp Ala gaa ttt Giu Phe 100 gaa agc gga Giu Ser Gly gac tgg tta Asp Trp Leu 120 aaa Lys 105 att agc gca tca Ile Ser Ala Ser ggt acc cga gcc aaa gag att gcg Gly Thr Arg Ala Lys Giu Ile Ala 110 115 tagatttcct aatgctcatt agt cca gtg gtc aaa Pro Val Val Lys <210> 1092 <211> 125 <212> PRT <213> Corynebacteriun glutamicum <400> 1092 Met Arg Ile Asp Pro Leu Giu Thr Arg Gin Ala Val Leu Ala Val Lys 10 1490 WO 01/00843 WO 0100843PCT/iBOO/00923 Asp Trp Ile Glu Gly Giu Gly Asp Val Lys Lys 25 Leu Ala Ala Ala -Thr Arg Leu Ser Val Arg Leu 40 Pro Gly Asn Ser Val Glu Val Arg Val Pro Pro Cy Ile Giu Gly Pro Lys His Thr Arg Gly Thr 70 Giu Thr Asp Ala Lys Thr Trp Leu Arg Leu Ala Phe Asp Ala Glu Phe Glu Ser Gly Lys Ile Ser 100 105 Ala Lys Glu Ile Ala Asp Trp Leu Pro Val Val 115 120 <210> 1093 <211> 1305 <212> DNA <2 13> Corynebacterium glutamicun <220> <221> CDS <222> (101)..(1282) <223> RXCO1088 <400> 1093 tgccttgagt ctaattctcc cgcccgtgcg atgggtttaa gtgaaccgaa ttttttaact gatttgaaga agcgagaata Gly Ala Val1 Pro Gly Ser Leu 125 Arg Gin Ala Asn Gin G ly 110 gca acc gct Ala Thr Ala gca ggt gca Ala Gly Ala ccc ggt gac Pro Gly Asp cga ggc atc Arg Gly Ilie cgc cac tta Arg His Leu gaa caa acc ggc Gly gaa Giu gtg Val1 aag Lys agc Ser aag gtt Val1 cta Leu ctc Leu ctg Leu acc Thr cag ggg Gly aaa Lys cgc Arg 45 gtg Val aaa Lys gcc ga t Asp atc Ile 30 acc Thr gac Asp gaa Giu tta 1491 gctggactga taaacctttt atg gga ctg tgg atc Met Gly Leu Trp Ile 1 ctg gga gca ctc att Leu Giy Ala Leu Ile gtt gtg gaa gca gtc Val Val Giu Ala Val gta gtg cgc caa ggc Val Val Arg Gin Giy cat gaa cac cat cat His Glu His His His gtc aat gct gac atc Val Asn Ala Asp Ile ttt gaa ctc atc gct 115 163 211 259 307 355 403 WO 01/00843 Pro Glu Gin atc gct gaa Ile Ala Giu cat gag gta His Glu Vai 120 gaa gcg atc Glu Ala Ile 135 gct tta gga Ala Leu Giy 150 cca gtt cca Pro Val Pro gga gct ctt Giy Ala Leu act ggt gtt Thr Gly Val 200 cca ggt ggc Pro Giy Gly 215 aca gaa ggc Thr Giu Gly 230 agg agt aac Arg Ser Asn gat gat caa Asp Asp Gin gcc gct ggc Ala Ala Gly 280 ggc cgt cct 1027 Gly Arg Pro 295 gaa gca gtg 1075 Giu Ala Val 310 Thr gga Gly 105 gga Gly agg Arg ttc Phe gcc Ala atg Met 185 gcg Ala atc Ile cgt Arg cca Pro gac Asp 265 gca Ala gca Ala aaa Lys Lys aaa Lys gct Ala cag Gin gga Gly gtg Val 170 gag Glu ttg Leu atc Ile cca Pro gat Asp 250 cca Pro gta Val cat His ac c Thr Gin gtc Val1 t gg Trp ctt Leu cgc Arg 155 gca Ala agc Ser atc Ile aat Asn aat Asn 235 acc Thr Cgg Arg gat Asp agg Arg gca Ala 315 Asp cac His gat Asp aac Asn 140 atc Ile gag Giu acc Thr cg t Arg gaa Glu 220 ata Ile cgc Arg ctg Leu gca Ala gtg Val1 300 tta Leu Ala ggc Gly tcc Ser 125 c ca Pro aag Lys ctg Leu gaa Giu cac His 205 gtt Val gtg Val aca Thr tgg Trp tgg Trp, 285 tca Ser ttt Phe Leu atc Ile 110 at c Ile ggt Gly gca Ala gtg Val cct Pro 190 ttt Phe ggc Gly cgc Arg ctg Leu cc a Pro 270 Ctg Leu gca Ala gca Ala Gly 95 gag Glu gca Ala ttg Leu gc t Ala aaa Lys 175 gtt Val gcc Ala att Ile gca Ala gtg Val 255 gga Gly act Thr t tg Leu gcc Val ccg Pro gac Asp att I le cac His 160 ggc Gly ggt Gly acc Thr gg t Gly att Ile 240 caa Gin gta Val1 cca Pro gtg Val acc Phe gag Giu att Ile gct Ala 145 gga Gly tgg Trp gaa Giu caa Gin gca Ala 225 ttg Leu t ta Leu ata Ile at t Ile gat Asp 305 acg Glu aaa Lys gtg Val1 130 gca Ala gat Asp ccc Pro tta Leu gat Asp 210 gga Gly ttc Phe gaa Giu gag Giu ttg Leu 290 agc Ser act Leu atc Ile 115 ggt Gly tct Ser att Ile acc Thr gcc Ala 195 ggc G ly aca Thr aac Asn gcc Ala atc Ile 275 atg Met tcc Ser ttt PCTIBOO/00923 Ile Ala 100 cac ttc 451 His Phe gtg tgc 499 Val Cys ccg att 547 Pro Ile cca gtg 595 Pro Val 165 caa acc 643 Gin Thr 180 acc cca 691 Thr Pro cct ttc 739 Pro Phe aaa gat 787.

Lys Asp acc tct 835 Thr Ser 245 aat gtt 883 Asn Val 260 ctc ttt 931 Leu Phe aag aag 979 Lys Lys gag gtg Glu Val ggg atc Ala Thr Thr Thr Phe Gly Ile 320 325 1492 WO 01/00843 aga tca tgg gaa gtc gaa cga gaa ggc ttg gac cgt 1123 Arg Ser Trp Giu Val Glu Arg Giu Gly Leu Asp Arg 330 335 gtc gag gtg gac gga cac acc atc aac atc aaa atc 1171 Val Giu Val Asp Gly His Thr Ile Asn Ilie Lys Ile 345 350 gat caa gta atc agt gca cag tcc gag ttt gaa gat 1219 Asp Gin Val Ilie Ser Ala Gin Ser Glu Phe Glu Asp 360 365 gcg gtg gcc ttg gga att tca gag cgg gaa gtt gtg 1267 Ala Val Ala Leu Giy Ilie Ser Glu Arg Giu Val Val 375 380 385 caa ggc acc acc gag taacaaccaa aaggtcgact gct 1305 Gin Gly Thr Thr Giu 390 <210> 1094 <211> 394 <212> PRT <213> Corynebacterium giutanicum PCTIiBOO/00923 gaa caa Glu Gin 340 cgt gat Arg Asp tct gca Ser Ala att ccg Ile Pro <400> 1094 Met Gly Leu Trp Ile Asp Ala Thr Ala 1 Leu Val Val His Val Phe Giu Ile Ala 145 Ala Glu Arg His Ala Leu Ile 115 Gly Ser Leu Ala Gin His Asp Ile 100 His Val1 Pro 5 Ile Val Gly His Ile Ala Phe Cys Ile Asp Ile Gin His 70 Pro Ile His Glu Ala 150 Ala 25 Asp Ile Leu Thr Gly 105 Gly Arg Phe Gly 10 Giu Val1 Lys Ser Lys 90 Lys Ala Gin Gly Ala Glu Leu His Ile Asp His Asp Asn 140 Ile Gly Lys Arg Val Lys Ala Gly Ser 125 Pro Lys Asp Ile Thr Asp Giu Leu Ile 110 Ile G ly Ala Met Gin Giu Ala Leu Gly Giu Ala Leu Ala Leu Gin Glu Gin Leu Val1 Pro Asp Ile His 160 1493 WO 01/00843 WO 0100843PCTLBOO/00923 Gly Asp Ile Pro Val Pro Val Pro Ala Val Ala Giu Leu Val Lys Gly 165 170 175 Trp Pro Thr Gin Thr Gly Ala Leu Met Glu Ser Thr Glu Pro Val Gly 180 185 190 Giu Leu Ala Thr Pro Thr Gly Val Ala Leu Ile Arg His Phe Ala Thr- 195 200 205 Gin Asp Gly Pro Phe Pro Gly Gly Ile Ile Asn Giu Val Gly Ile Gly 210 215 220 Ala Gly Thr Lys Asp Thr Glu Gly Arg Pro Asn Ile Val Arg Ala Ile 225 230 235 240 Leu Phe Asn Thr Ser Arg Ser Asn Pro Asp Thr Arg Thr Leu Vai Gin 245 250 255 Leu Giu Ala Asn Val Asp Asp Gin Asp Pro Arg Leu Trp Pro Gly Val 260 265 270 Ile Giu Ile Leu Phe Ala Ala Gly Ala Val Asp Ala Trp Leu Thr Pro 275 280 285 Ile Leu Met Lys Lys Gly Arg Pro Ala His Arg Val Ser Ala Leu Val 290 295 300 Asp Ser Ser Giu Val Glu Ala Val Lys Thr Ala Leu Phe Ala Ala Thr 305 310 315 320 Thr Thr Phe Gly Ile Arg Ser Trp Glu Val Giu Arg Giu Gly Leu Asp 325 330 335 Arg Arg Phe Glu Gin Val Giu Val Asp Gly His Thr Ile Asn Ile Lys 340 345 350 Ile Gly Ser Arg Asp Asp Gin Vai Ile Ser Ala Gin Ser Giu Phe Giu 355 360 365 Asp Ile Arg Ser Ala Ala Val Ala Leu Gly Ile Ser Giu Arg Glu Val 370 375 380 Val Ala Arg Ile Pro Gin Gly Thr Thr Glu 385 390 <210> 1095 <211> 1419 <212> DNA <213> Corynebacterium glutaxnicum <220> <221> CDS <222> (101)..(1396) <223> RXC02624 <400> 1095 acccggtcag atccgacgtc gccggccaaa accgcaagca cctgcgcaaa cgccacagaa ggcagctcag caatcaaagt tgctgcgttt ccttccaacc gtg ctg att ccg cat 115 1494 WO 01/00843 WO 0100843PCTIBOOIOO923 Val Leu Ile Pro 1 ggc gtg gcg gtg Gly Val Ala Val ctt Leu ttg gtt att att ctc gcc gta gcc tcc Leu Val Ile Ile Leu Ala Val Ala Ser 15 cta atg Leu Met ttc acc aat Phe Thr Asn tgg ctt tcc Trp Leu Ser tca atg gtg aat Ser Met Val Asn tcg gca acg att Ser Ala Thr Ile gca cag ctg Ala Gin Leu gaa gtg atc Giu Val Ile cta aat ctc ggt Leu Asn Leu Gly gtg gac ggc agt Val Asp Gly Ser ggg Gly tca gta Ser Val ctg ccc acg ctt Leu Pro Thr Leu ggc ttt ata ttc Gly Phe Ile Phe tgg gcc atc gcc Trp Ala Ile Ala gcg Ala cgc atc cac cgc Arg Ile His Arg gtc aaa gat cgt Val Lys Asp Arg agc atc gcc gac Ser Ile Ala Asp ggc gtc ctc gca Gly Vai Leu Ala gca Ala ctc gtc ctc ggc Leu Val Leu Gly ccg ctt gcg ctc Pro Leu Ala Leu acc gcc Thr Ala 100 atc gca gcg Ile Ala Ala gtc ccg cca Val Pro Pro 120 atg ctt ttc gac Met Leu Phe Asp tcc agc gtc ctc Ser Ser Val Leu aac gtc gag Asn Val Giu 115 cac ctc agc His Leu Ser atc acg cgc ctc Ile Thr Arg Leu cgc gtg atg ttg Arg Val Met Leu ttc Phe 130 gcc ctc Ala Leu 135 ttc ctc ggc atg ggg cca cgc ctg tgg Phe Leu Gly Met Gly Pro Arg Leu Trp, 140 gcg ttg gcg cgc Ala Leu Ala Arg cgc Arg 150 tac ggt gct cca Tyr Gly Ala Pro gaa Giu 155 tgg ctt atc gac Trp Leu Ile Asp gcc Ala 160 atc acc caa gct Ile Thr Gin Ala cgc ttc ctc atc Arg Phe Leu Ilie gca.

Ala 170 ttt gga aca gtc Phe Gly Thr Val ttg gtt tcc gtg Leu Val Ser Val ctc gtg Leu Val 180 atg acc gcg Met Thr Ala gac gac tcc Asp Asp Ser 200 aac cac agt gca ttc acc gcg acc atg Asn His Ser Ala Phe Thr Ala Thr Met 190 cag ggt tac Gin Gly Tyr 195 att ctg tat Ile Leu Tyr gcC tct gtt gtg Ala Ser Val Val ttg atc gtc ctg Leu Ile Val Leu ctg ccc Leu Pro 215 aac atg atg atc Asn Met Met Ile gcg atg ggc aat Ala Met Gly Asn ctg Leu 225 atc ggc tca ccc Ile Giy Ser Pro ctt tac ttc ggt gac gcc tcc atc agc gtc ttc agc gtg cat tcc gtt Leu Tyr Phe Gly Asp Ala Ser Ile Ser Val Phe Ser Val His Ser Val 835 1495 WO 01/00843 230 cca ttg cca Pro Leu Pro tca tgg gca Ser Trp, Ala gtc tgc gtg Val Cys Val 280 gtc att tca 1027 Val Ile Ser 295 acc ttg ggc 1075 Thr Leu Gly 310 ggc cta gtt 1123 Gly Leu Val atc gac aag 1171 Ile Asp Lys gct gtt gtg 1219 Ala Val Val 360 cat gtt gaa 1267 His Val Giu 375 gta gaa gag 1315 Val Giu Glu 390 gaa gag gaa 1363 Glu Glu Giu gaa acc aat 1416 Glu Thr Asn c tg 1419 ccg Pro gtg Val 265 aga Arg gca Ala gta Val ttc Phe ctg Leu 345 gag Glu gaa Glu gtc Val1 gaa Glu gat Asp 425 235 ccc Pro t ta Leu ccc Pro tgt Cys aac Asn 315 tat Tyr aac Asn gag Glu gta Val gaa Glu 395 gac Asp tcc Ser atc Ile ctg Leu atg Met ttc Phe 300 tac Tyr ttc Phe cc t Pro cct Pro gat Asp 380 gac Asp gaa Glu gag Glu 240 gca gct ctc Ala Ala Leu* 255 atc cct gca Ile Pro Ala 270 ctt gcc gtg Leu Ala Val gtc ctg gca Val Leu Ala gga ctc aac Gly Leu Asn 320 ctc gtt gga Leu Val Gly 335 gaa gtt aag Glu Val Lys 350 gaa gtt gaa Glu Val Glu gag gaa gag Glu Glu Glu gca gag-gat Ala Glu Asp 400 att gag aca Ile Glu Thr 415 gaa gac cgt Glu Asp Arg 430 PCTIIBOOIOO923 245 ccc agc gaa gcc ctc 883 Pro Ser Glu Ala Leu 260 att att gcc acc tgg 931 Ile Ile Ala Thr Trp, 275 aac aca aca gca gca 979 Asn Thr Thr Ala Ala 290 gtt ttc gcc ggc gga Val Phe Ala Gly Gly 305 ctc ctg gcg tca gtt Leu Leu Ala Ser Val 325 ctc ctc atc gcc gga Leu Leu Ile Ala Gly 340 tct gtt aag gct gtg.

Ser Val Lys Ala Val 355 gag gac gaa gag gag Glu Asp Glu Glu Glu 370 gaa gtt gag gaa ggg Glu Val Glu Glu Gly 385 cct gaa gag aat cct Pro Glu Glu Asn Pro 405 gaa act gag gct gaa Glu Thr Glu Ala Glu 420 taacatatct gttgtgaatt 1496 WO 01/00843 WO 0100843PCTIBOO/00923 <210> 1096 <211> 432 <212> PRT <213> Corynebacterium giutamicum <400> 1096 Val Leu Ile Pro His Gly Val Ala Val Leu Leu Val Ile Ile Leu Ala 1 5 10 Val Ala Ser Leu Met Phe Thr Asn Ser Ser Met Val Asn Leu Ser Ala 25 Thr Ile Ala Gin Leu Trp Leu Ser Leu Asn Leu Gly Ala Val Asp Gly 40 Ser Gly Glu Val Ile Ser Val Leu Pro Thr Leu Pro Gly Phe Ile Phe 55 Leu Trp Ala Ile Ala Ala Arg Ile His Arg Ala Vai Lys Asp Arg Val 70 75 Ser Ile Ala Asp Leu Gly Val Leu Ala Ala Leu Val Leu Gly Ile Pro 90 Leu Ala Leu Thr Ala Ile Ala Ala Phe Met Leu Phe Asp Ala Ser Ser 100 105 110 Val Leu Asn Val Glu Val Pro Pro Ile Thr Arg Leu Leu Arg Val Met 115 120 125 Leu Phe His Leu Ser Ala Leu Phe Leu Gly Met Gly Pro Arg Leu Trp 130 135 140 Gin Ala Leu Ala Arg Arg Tyr Gly Ala Pro Glu Trp Leu Ile Asp Ala 145 150 155 160 Ile Thr Gin Ala Phe Arg Phe Leu Ile Ala Phe Gly Thr Val Ser Leu 165 170 175 Val Ser Val Leu Val Met Thr Ala Ile Asn His Ser Ala Phe Thr Ala 180 185 190 Thr Met Gin Gly Tyr Asp Asp Ser Ala Ser Val Val Ala Leu Ile Val 195 200 205 Leu Ser Ile Leu Tyr Leu Pro Asn Met Met Ile Phe Ala Met Gly Asn 210 215 220 Leu Ile Gly Ser Pro Leu Tyr Phe Gly Asp Ala Ser Ile Ser Val Phe 225 230 235 240 Ser Val His Ser Val Pro Leu Pro Pro Leu Pro Ile Leu Ala Ala Leu 245 250 255 Pro Ser Giu Ala Leu Ser Trp Ala Val Ala Leu Leu Val Ile Pro Ala 260 265 270 Ile Ilie Ala Thr Trp Val Cys Val Arg Asn Pro Met Arg Leu Ala Val 275 280 285 1497 WO 01/00843 WO 0100843PCT/EBOO/00923 Asn Val 305 Leu Leu Ser Giu Glu 385 Pro Glu Thr 290 Phe Leu Leu Val1 Asp 370 Val Glu Thr Ala Gly Ser Ala 340 Ala Giu Glu Asn Ala 420 Ala Gly Val1 325 Gly Val1 Giu Gly Pro 405 Glu Val1 Thr 310 Gly Ile Ala His Val1 390 Giu Glu Ser Gly Val Lys Val1 360 Glu Glu Glu Ash Ala Val Phe Leu 345 Giu Glu Val Giu Asp 425 Val1 Gly Leu Glu 350 Glu Glu Ala Ile Giu 430 Leu Leu Val1 335 Val Val Giu Glu Giu 415 Asp Ala Asn 320 Gly Lys Glu Giu Asp 400 Thr Arg <210> 1097 <211> 603 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS <222> (101)..(580) <223> RXC02665 <400> 1097 caaggcgacc caatggcgtt taaagtaaca acccccattg atcaccgacg aagccgaacc cacaatattt gaggtaccag atc ccc cgc gta gga atc gcc aca gac gcc cac Ile Pro Arg Val Gly Ile Ala Thr Asp Ala His 15 aaa ccc tgc tgg atc gcc tgc ctc ctc ttt gaa Lys Pro Cys Trp Ile Ala Cys Leu Leu Phe Glu 30 gaa ggc cac tcc gac ggt gat gtt gta gct cat Glu Gly His Ser Asp Gly Asp Val Val Ala His 45 atatgatgct ggcacaacgc gtg act aac cca atc 115 Val Thr Asn Pro Ile 1 caa atc gaa gcg gga 163 Gin Ile Giu Ala Gly ggc gtc gac ggc tgc 211 Gly Val Asp Gly Cys gca att gtg gat gct 259 Ala Ile Val Asp Ala 1498 WO 01/00843 ctc ctt Leu Leu ggg aga Gly Arg gtt cgg Val Arg gcc caa Ala Gin gca caa Ala Gin gcc acc Ala Thr 135 tct gcc tct ggt Ser Ala Ser Gly cct Pro gag Glu ctg Leu caa Gin 120 acc Thr ggg G ly gtt Val1 cac Hius ccc Pro atc Ile 125 gga Gly gat Asp tct Ser ggg Gly aaa Lys 110 atc Ile t tc Phe ttg Leu ggt Gly tac Tyr ttt Phe PCT/IB00100923 tct ttc gtt ggt gtg 307 Ser Phe Val Gly Val cag ttg ttg aag gaa 355 Gin Leu Leu Lys Giu att gga aat gtc gcc 403 Ile Gly Asn Val Ala 100 ccc cgc cgc gaa gaa 451 Pro Arg Arg Giu Giu 115 cca tgc tca ctg tct 499 Pro Cys Ser Leu Ser 130 cgc agc gag ggt cgt 547 Arg Ser Giu Gly Arg 145 taagttttct gtagggattg 600 ggc gca Gly Ala act ggt Thr Gly aag gct Lys Ala 160 gca tcg gta gca acg gca gtg gtg tgg Ala Ser Val Ala Thr Ala Val Val Trp 150 155 99c <210> 1098 <211> 160 <212> PRT <213> Corynebacteriun giutamicum <400> 1098 Val Thr Asn Pro Ile Ile Pro Arg Val 1 5 Gin Ile Giu Ala Gly Lys Pro Cys Trp 25 Gly Val Asp Gly Cys Giu Gly His Ser 40 Ala Ile Val Asp Ala Leu Leu Ser Ala Ser Phe Val Gly Val Gly Arg Pro Giu Gin Leu Leu Lys Glu Val Arg Glu Leu Ile Gly Asn Val Ala Ala Gin Leu Val 100 105 Pro Arg Arg Giu Giu Ala Gin Gin Val 115 120 Gly Ile Asp Ser Tyr Leu Gly Ile Thr Leu Vai G ly Val1 His Pro Ile 125 Asp Leu Val1 Asp Ser Giy Lys 110 Ile 1499 WO 01/00843 PCT/LB00100923 Pro Cys Ser Leu Ser Ala Thr Thr Thr Asp His Met Gly Phe Thr Gly 130 135 140 Arg Ser Glu Gly Arg Ala Ser Val Ala Thr Ala Val Val Trp Lys Ala 145 150 155 160 <210> 1099 <211> 1689 <212> DNA <213> Corynebacterium glutanicun <220> <221> CDS <222> (101)..(1666) <223> RXC02770 <400> 1099 tcgccggggc aaaaaccgta taattacagt cctattacga ttcggggaaa ttcacacatg ttgtttcgga agtcacgcag cgcggtaatc atg ttg gtt ggctgggtac gca gcg Ala Ala tta Leu caa Gin aac Asn gcc Ala att Ile cgc Arg cag Gin atg Met cga Arg gtg Val1 aca Thr gcg Ala aga Arg ccc Pro cag G in cct Pro ccg Pro gtg Val1 agc Ser ttc Phe c tg Leu ccg Pro gat Asp t ac Tyr tgt Cys t tc Phe gta Val tgt Cys ggc Gly t tg Leu ggt G ly ctt Leu 75 act Thr gat Asp cag Gin tc t Ser ggt Gly tac Tyr gga Gly gtg Val 60 gct Ala atc Ile gat Asp tcc Ser ggt Gly ga t Asp gca Ala gtg Val tat Tyr tcc Ser aat Asn ttt Phe cat His 125 tct Ser ggg Gly gtt Val gct Ala gt t Val1 acg Thr gaa Giu ctg Leu 110 gtg Val aaa Lys gaa ccg Giu Pro 15 aac tct Asn Ser aat gat Asn Asp cag ggt Gin Gly cag gta Gin Val 80 gat gcc Asp Ala 95 ctc tct Leu Ser cca ttg Pro Leu gta gcc Val Ala Met Leu Val 1 gaa cca acc Giu pro Thr tcg ctg gct Ser Leu Ala 35 gct ggt ctt Ala Gly Leu 50 cct tct ggg Pro Ser Gly 65 ttg ccg ggt Leu Pro Gly acc tac tca Thr Tyr Ser gcg aca gct Ala Thr Ala 115 acc tcg cag Thr Ser Gin 12an agc Ser 20 aca Thr ttg Leu cag Gin att Ile gat Asp 100 ggg Gly att Ile cac His acc Thr gct Ala atg Met aac Asn ggt Gly cag Gin gag Glu 115 163 211 259 307 355 403 451 499 547 acc gtg gtg ttc aag Thr Val Val Phe Lys 1500 WO 01/00843 PCTIIBOO/00923 135 140 145 gaa gac ctc ggt gag cgt tgg cgt tat ctt ttt gag cag ggc gat ttg 595 Glu Asp Leu Gly Glu Arg Trp Arg Tyr Leu Phe Giu Gin Gly Asp Leu 150 155 160 165 ttg cca gcc cat gcc gtt gct tcc aaa gca ggt atg acc ttg gag gag 643 Leu Pro Ala His Ala Val Ala Ser Lys Ala Gly Met Thr Leu Glu Giu 170 175 180 ctt aat cag gcg ttg aag gat aag gat cct gaa gcg ttg act gaa cct 691 Leu Asn Gin Ala Leu Lys Asp Lys Asp Pro Glu Ala Leu Thr Glu Pro 185 190 195 gct cgt gtg tgg agc gaa ggt ttc cag ctg tcc cag ttt gat cca gag 739 Ala Arg Val Trp Ser Giu Gly Phe Gin Leu Ser Gin Phe Asp Pro Glu 200 205 210 ctg cag acg gct ttt ggc ccg tac aag gtg gat tct gtg ggt gaa ttc 787 Leu Gin Thr Ala Phe Gly Pro Tyr Lys*Val Asp Ser Val Gly Giu Phe 215 220 225 ggc gaa gtc aag ctg gta cgc aat gag ttt tac agt ggc gac cag gcg 835 Gly Giu Val Lys Leu Val Arg Asn Giu Phe Tyr Ser Gly Asp Gin Ala 230 235 240 245 gtt gaa gca gaa atc acg atg tgg cct aaa ggc tcg gat ctc agc gcc 883 Val Giu Ala Giu Ile Thr Met Trp Pro Lys Gly Ser Asp Leu Ser Ala 250 255 260 att gcg gat aat gga aac ctt cag atc gca cat gtt gtg gcg tgg gag 931 Ile Ala Asp Asn Gly Asn Leu Gin Ile Aia His Val Val Ala Trp Glu 265 270 2.75 agc gag ccg tgg gta aat cgc gat gac cca ttg aat cct tat gac att 979 Ser Giu Pro Trp Val Asn Arg Asp Asp Pro Leu Asn Pro Tyr Asp Ile 280 285 290 aag gaa gag gtc ggt gtt ttg act gag cag ctc acc ttg gcc agt gcc 1027 Lys Giu Giu Val Giy Val Leu Thr Giu Gin Leu Thr Leu Ala Ser Ala 295 300 305 ggt gtg ttt tac gct gcg gag gcc cgg cag gcg ttt gcg gcc tgc gtt 1075 Gly Val Phe Tyr Ala Ala Giu Ala Arg Gin Ala Phe Ala Ala Cys Val 310 315 320 325 gac cag gaa gcg gtg gct gcg gcg tcg tca agc atc tct gga atc gat 1123 Asp Gin Giu Ala Val Ala Ala Ala Ser Ser Ser Ile Ser Gly Ile Asp 330 335 340 gtg cct gcc gta ggt gtg cac tcg gtg cgt cac caa aat ccg gtc gtg 1171 Val Pro Ala Val Gly Val His'Ser Val Arg His Gin Asn Pro Val Val 345 350 355 cac caa atc ggt gat ctg cca gca cag cac atg gcg gtg gat att aat 1219 His Gin Ilie Gly Asp Leu Pro Ala Gin His Met Ala Val AspIle Asn 1501 WO 01/00843 WO 0100843PCTIiBOO/00923 gcc gca 1267 Ala Ala 375 ccc gat 1315 Pro Asp 390 gag cct 1363 Glu Pro ctt aat 1411 Leu Asn tac ttc 1459 Tyr Phe cgg gag 1507 Arg Glu 455 agg cgc 1555 Arg Arg 470 gca gcg 1603 Ala Ala gtt gtt 1651 Val Val tcc aga 1689 Ser Arg tca gcg ttg gcg ggt Ser Ala Leu Ala Gly 380 gag cgc aag gct gca Giu Arg Lys Ala Ala 395 gcc ggt atc acc gtt Ala Gly Ile Thr Val 410 gat ctc agt cga acc Asp Leu Ser Arg Thr 365 caa Gin atg Met 370 tcc atc cgc att ggc tac gac gga Ser Ile Arg Ile Gly Tyr Asp Gly 385 gtg gag gcg att cgc caa agt tgt Val Glu Ala Ile Arg Gin Ser Cys atc gat gcg Ile Asp Ala 415 gaa gtc agt Giu Val Ser 400 405 tcg cag gag gct gtt agt Ser Gin Giu Ala Val Ser 420 gaa tgg ggc tat gag cag Giu Trp Gly Tyr Giu Gin 435 425 gaa ggg Giu Gly aca ctt. gac gct Thr Leu Asp Ala 445 aat gcc aat acc Asn Ala Asn Thr 430 gtt ctg cgt aca gtg gat cca cat Val Leu Arg Thr Val Asp Pro His 450 att gga act gat gcg gag tcg acg Ile Gly Thr Asp Ala Giu Ser Thr gaa 460 act gaa gaa caa Thr Giu Glu Gin 475 caa ccc cga gtg Gin Pro Arg Vai 490* aat aca gac cta Asn Thr Asp Leu 505 agt gag gaa taa Ser Giu Giu 520 ttg Leu tgg gct gaa gtc Trp Ala Giu Val 480 465 cca Pro tca att cca cta Ser Ile Pro Leu 485 ttt gtg ata gat Phe Val Ile Asp 495 gcc ggt atc gga Ala Giy Ile Gly cgc aca gtc ggt aac gtt Arg Thr Val Gly Asn Val 500 tgg aac atg gac cgt tgg Trp Asn Met Asp Arg Trp 515 gtagtga gcgaacaagc tct <210> 1100 <211> 522 <212> PRT <213> Corynebacterium giutainicum <400>, 1100 Met Leu Val Ala Ala Leu Val Met Thr Ser Cys Gly Asp Gly Giu Pro 1 5 10 1502 WO 01/00843 WO 0100843PCTIiBOO/00923 Glu Pro Thr Ser His Gin Thr Ser I~eu Phe Gly Tyr Ala Val Asn Ser 25 Ser Ala Pro Leu Thr Ala Thr Thr 145 Giu Met Ala Gin Ser 225 Ser Ser Val Asn Thr 305 Phe Leu Gly Ser Pro Tyr Thr Ser 130 Val1 Gin Thr Leu Phe 210 Val Gly Asp Val Pro 290 Leu Ala Ala Leu Gly Gly Ser Ala 115 Gin Val Gly Leu Thr 195 Asp Gly Asp Leu Ala 275 Tyr Ala Ala Thr Leu Gin Ile Asp 100 G ly Ile Phe Asp Glu 180 Giu Pro Giu Gin Ser 260 Trp Asp Ser Cys Thr Ala Met Asn Gly Gin Giu Lys Leu 165 Glu Pro Glu Phe Ala 245 Ala Giu Ile Ala Val 325 Asn Ala Ile 70 Arg Gin Met Arg Giu 150 Leu Leu Ala Leu Gly 230 Val1 Ile Ser Lys Gly 310 Asp Ser Tyr Thr Ile Val 105 Leu Cys Gly His Ala 185 Trp Ala Lys Giu Asn 265 Trp Val Tyr Ala Leu Pro Asp Tyr 90 Cys Phe Val Glu Ala 170 Leu Ser Phe Leu Ile 250 Gly Val1 Gly Ala Val 330 Leu Gly Leu 75 Thr Asp Gin Ser Arg 155 Val Lys Glu Gly Val 235 Thr Asn Asn Val Ala 315 Ala Gly Val1 Ala Ile Asp Ser Gly 140 Trp Ala Asp Gly Pro 220 Arg Met Leu Arg Leu 300 Giu Ala Val Tyr Ser Asn Phe His 125 Ser Arg Ser Lys Phe 205 Tyr Asn Trp Gin Asp 285 Thr Ala Ala Ala Val1 Thr Glu Leu 110 Val1 Lys Tyr Lys Asp 190 Gin Lys Giu Pro Ile 270 Asp Glu Arg Ser Asn Gin Gin Asp Leu Pro Val Leu Ala 175 Pro Leu Val Phe Lys 255 Ala Pro Gin Gin Ser 335 Asp Gly Val Ala Ser Leu Ala Phe 160 Gly Glu Ser Asp Tyr 240 Gly His Leu Leu Ala 320 Ser 1503 WO 01/00843 PCTIiBOO/00923 Ile Ser Gly Ile Asp Val.Pro Ala Val Gly Val His Ser Val Arg His 340 345 350 Gin Asn Pro Val Val His Gin Ile Gly Asp Leu Pro Ala Gin His Met 355 360 365 Ala Val Asp Ile Asn Ala Ala Ser Ala Leu Ala Gly Gin Ser Ile Arg 370 375 380 Ile Gly Tyr Asp Giy Pro Asp Glu Arg Lys Ala Ala Met Val Giu Ala 385 390 395 400 Ile Arg Gin Ser Cys Giu Pro Ala Gly Ile Thr Vai Ile Asp Ala Ser 405 410 415 Gin Giu Ala Val Ser Leu Asn Asp Leu Ser Arg Thr Giu Val Ser Giu 420 425 430 Trp Giy Tyr Giu Gin Tyr Phe Giu Gly Thr Leu Asp Ala Vai Leu Arg 435 440 445 Thr Val Asp Pro His Arg Giu Tyr Giu Asn Ala Asn Thr Ile Gly Thr 450 455 460 Asp Ala Giu Ser Thr Arg Arg Thr Giu Giu Gin Leu Trp Ala Giu Val 465 470 475 480 Pro Ser Ilie Pro Leu Ala Ala Gin Pro Arg Val Phe Val Ile Asp Arg 485 490 495 Thr Val Giy Asn Val Val Val Asn Thr Asp Leu Ala Gly Ile Gly Trp 500 505 510 Asn Met Asp Arg Trp Ser Arg Ser Giu Giu 515 520 <210> 1101 <211> 408 <212> DNA <213> Corynebacterium giutainicum <220> <221> CDS <222> (101)..(385) <223> RXC02238 <400> 1101 ggcgcttagc caaaacatag agcggtaggg tatgcttatc cgattgagca acctttcccg ctcttaacac tactgtccat atacttttga aaaggtgtca gtg acc aac gtg agc 115 Val Thr Asn Val Ser 1 aac gag acc aac gcc acc aag gcc gtc ttc gat ccg cca gtg ggc att 163 Asn Giu Thr Asn Ala Thr Lys Ala Val Phe Asp Pro Pro Val Gly Ile 15 acc gct cct ccg atc gat gaa ctg ctg gat aag gtc act tcc aag tac 211 Thr Ala Pro Pro Ile Asp Giu Leu Leu Asp Lys Val Thr Ser Lys Tyr 30 1504 WO 01/00843 WO 0100843PCT/EBOO/00923 gcc ctc gtg atc ttc gca gcc aag cgt gcg cgc cag atc aac agc ttc 259 Ala Leu Val Ile Phe Ala Ala Lys Arg Ala Arg Gin Ile Asn Ser Phe 45 tac cat cag gca gat gag gga gta ttc gag ttc atc gga cca ttg gtt 307 Tyr His Gin Ala Asp Giu Gly Val Phe Giu Phe Ile Gly Pro Leu Val 60 act ccg cag cca ggc gaa aag cca ctt tct att gct ctg cgt gag atc 355 Thr Pro Gin Pro Gly Giu Lys Pro Leu Ser Ile Ala Leu Arg Giu Ile 75 80 aat gca ggt ctg ttg gac cac gag gaa ggt taaaagacct tataacttca 405 Asn Ala Gly Leu Leu Asp His Giu Giu Gly c ac 408 <210> 1102 <211> <212> PRT <213> Corynebacteriun giutanticum <400> 1102 Vai Thr Asn Val Ser Asn Giu Thr Asn Ala Thr Lys Ala Val Phe Asp 1 5 10 Pro Pro Vai Giy Ile Thr Ala Pro Pro Ile Asp Giu Leu Leu Asp Lys 25 Val Thr Ser Lys Tyr Ala Leu Val Ile Phe Ala Ala Lys Arg Ala Arg 40 Gin Ile Asn Ser Phe Tyr His Gin Ala Asp Giu Gly Val Phe Giu Phe 55 Ilie Gly Pro Leu Val Thr Pro Gin Pro Gly Giu Lys Pro Leu Ser Ile 70 75 Ala Leu Arg Glu Ilie Asn Ala Giy Leu Leu Asp His Giu Glu Gly 90 <210> 1103 <211> 1298 <212> DNA <213> Corynebacterium giutanicum <22 0> <221> CDS <222> (1)..(1275) <223> RXC01946 <400> 1103 atc cgc aag tac tcc agg ctc gag gaa caa ttc cag tcg ctc ggc ggc 48 Ile Arg Lys Tyr Ser Arg Leu Giu Giu Gin Phe Gin Ser Leu Gly Gly 1 5 10 tac gaa gct gac gcc gaa gca gcc cag atc tgc gac aac ctc ggc ctc 96 1505 WO 01/00843 Tyr Giu Ala gag gca cgc Giu Ala Arg cgc cgc cgc Arg Arg Arg tcc ggc aaa Ser Gly Lys ttg gac gca Leu Asp Ala gaa ggt gga Glu Gly Gly gta tgt aac Val Cys Asn 115 gtc tac aac Val Tyr Asn 130 gaa gca cgc Glu Ala Arg 145 gcc ctc aag Ala Leu Lys gcc gca gct Ala Ala Ala ctc gac gaa Leu Asp Giu 195 gaa cca gca Glu Pro Ala 210 aag atg tac Lys Met Tyr 225 gac aaa ggc Asp Lys Gly acc acc ctg Asp atc Ile gtc Val tca S er gac Asp ctg Leu 100 aag Lys atg Met cga Arg gac Asp aag Lys 180 atc Ile ccc Pro ggc Gly tcc Ser ctc Ala ctc Leu gag Glu aaa Lys tcg Ser atc Ile att Ile Gly cgc Arg cag Gin 165 cag Gin cgc Arg tgt Cys tcc Ser cgc Arg 245 aaa Glu gac Asp t tg Leu acc Thr 70 atc Ile atg Met tgg Trp ttt Phe cgt Arg 150 gc t Ala atg Met gta Val gga Gly ctc Leu 230 gta Val1 ctc Ala cag Gin gcg Ala 55 aca Thr ac c Thr at t Ile tac Tyr agc Ser 135 gag Glu gca Ala atc Ile gc t Ala aaa Lys 215 gaa Glu gtc Val ctc Ala cag Gin cag Gin t tg Leu tgg Trp tcg Ser ctc Leu 120 aaa Lys cgc Arg cgc Arg gcc Ala gac Asp 200 acc Thr gtc Val gtc Val1 gcc Gin 25 ctt Leu atc Ile ctt Leu ctc Leu cac His 105 gac Asp tac Tyr gca Aia ctc Leu cgt Arg 185 cgc Arg cca Pro t tc Phe C tc Leu ggt Ile aaa Lys C tc Leu ctc Leu cgt Arg 90 gac Asp gca Al a gtc Val aac Asn 99C Gly 170 gcg Ala gcc Ala ctc Leu gcc Al a gga Gly 250 gtg Cys ac c Thr ttc Phe gac Asp 75 gac Asp gtc Val1 gta Val1 gat Asp gcc Ala 155 gcg Ala gaa Glu gcc Ala aac Asn ggc Gly 235 t tc Phe gaa Asp c tg Leu gcc Ala gag Giu ttc Phe gaa Giu cgc Arg gca Ala 140 gaa Glu aaa Lys cga Arg aac Asn gcc Ala 220 gtc Val aac Asn cgc Leu ggC Gly acc Thr acc Thr gcg Ala ctt Leu 110 gaa Glu gca Ala aag Lys acc Thr atc Ile 190 gtt Val ggC Gly cta Leu gca Ala gac PCT/iBOO/00923 Gly Leu ggc cag 144 Gly Gin aac ggc 192 Asn Gly aac cac 240 Asn His aag cac 288 Lys His ggc gcc 336 Gly Ala gcc gat 384 Ala Asp ctc gat 432 Leu Asp gcc gga 480 Ala Gly 160 aag gct 528 Lys Ala 175 gac aac 576 Asp Asn ttc cca 624 Phe Pro ctg acc 672 Leu Thr gcc atc 720 Ala Ile 240 ggt aaa 768 Gly LYS 255 ggc gaa 816 Gly Giu Thr Thr Leu Leu Lys Leu Leu Ala Gly Vai Giu Arg Thr Asp 1506 WO 01/00843 WO 0100843PCT/iBOO/00923 ggc ggc atc Gly Giy Ile 275 acc gga tac Thr Gly Tyr aaa atc ggc Lys Ile Giy 270 ttc gcc cag Phe Ala Gin aac acc atc Asn Thr Ile gaa cac Giu His 290 gaa gcc Giu Ala 305 tcc ttc 1008 Ser Phe tcc ggc 1056 Ser Giy cgc gca 1104 Arg Ala atc tcc 1152 Ile Ser 370 gtc gtc 1200 Val Val 385 gaa cgc 1248 Giu Arg cag tac 1295 Gin Tyr gac Asp acc atc gac Thr Ile Asp ccc Pro 295 gac Asp gac aaa tcc gtc Asp Lys Ser Val tgc gcc gac Cys Aia Asp atg ttc tcc Met Phe Ser 325 caa caa agc Gin Gin Ser agc ctc ctc Ser Leu Leu gaa caa ctc gac Giu Gin Leu Asp 330 cca gca gga aca ctc Pro Ala Gly Thr Leu 335 ggt gaa Gly Giu 340 aac gtc Asn Val aaa acc cgc ctc Lys Thr Arg Leu ctg ctt ctc gac Leu Leu Leu Asp 360 cag gtc ctc gac Gin Vai Leu Asp gca ctg gcc acc ctc gtg tcc tcc Aia Leu Ala Thr Leu Vai Ser Ser 345 350 gag ccc acc aac aac ctt gac ccg Giu Pro Thr Asn Asn Leu Asp Pro 365 gca ctg cgc acc tac acc ggc gca Aia Leu Arg Thr Tyr Thr Giy Ala 380 ggt gca gtc aag gcc ctt gag cca Gly Ala Val Lys Ala Leu Giu Pro gaa Glu 375 ctg gtt acc cac Leu Val Thr His 390 gac ccg Asp Pro gtc atc gtg ctt cct gat ggc Val Ilie Val Leu Pro Asp Gly 405 atg gaa atc gtg gaa ttg gcg Met Giu Ile Val Giu Leu Ala 395 400 acc gag gat ctt tgg aat gat Thr Giu Asp Leu Trp Asn Asp taggttctaa ggctgtttat gct 1298 <210> 1104 <211> 425 <212> PRT <213> Corynebacteriui giutamicum <400> 1104 Ile Arg Lys Tyr Ser Arg Leu Giu Glu Gin Phe Gin Ser Leu Giy Gly 1 5 10 1507 WO 01/00843 WO 0100843PCTIBOO/00923 Tyr Giu Arg Ser Leu Glu Val Val Giu 145 Ala Ala Leu Giu Lys 225 Asp Thr Gly Giu Giu 305 Ser Ala Leu Giu Lys Ser Ile Ile Gly Arg Gin 165 Gin Arg Cys Ser Arg 245 Lys Thr Ile Asp Ser 325 Ala Gin Ala Thr Thr Ile Tyr Ser 135 Giu Ala Ile Ala Lys 215 Giu Val1 Leu Tyr Pro 295 Asp Glu Ala Gin Gin Leu Trp Ser Leu 120 Lys Arg Arg Ala Asp 200 Thr Val Val1 Ala Gly 280 Asp Gin Gin Gin Leu Ile Leu Leu His 105 Asp Tyr Ala Leu Arg 185 Arg Pro Phe Leu Gly 265 Leu Lys Gin Leu Ile Lys Leu Leu Arg 90 Asp Ala Val Asn Gly 170 Ala Ala Leu Ala Gly 250 Val Lys Ser Ser Asp 330 Cys Thr Phe Asp 75 Asp Val1 Val Asp Ala 155 Ala Giu Ala Asn Gly 235 Phe Giu Ile Val Leu 315 Gin Asp Leu Ala Glu Phe Giu Arg Ala 140 Giu Lys Arg Asn Ala 220 Val Asn Arg Gly Trp 300 Arg Pro Gly Gly Asn Asn Lys Gly Ala Leu Ala Lys 175 Asp Phe Leu Ala Gly 255 Gly Ala Thr Leu Thr 335 Leu Gin Gly His His Ala Asp Asp Gly 160 Ala Asn Pro Thr Ile 240 Lys Giu Gin Ile Gly 320 Leu 1508 WO 01/00843 Ser Gly Gly Arg Ala Asn 355 Ile Ser Arg 370 Val Vai Leu 385 Giu Arg Val Gin Tyr Met Lys Leu Gin Thr Val1 405 Ile Thr Leu Val His 390 Leu Val Arg Leu Leu 375 Asp Pro Giu Ala 345 Glu Ala Giy Giy Aia 425 Ala Thr Leu Vai 350 Thr Asn Asn Leu 365 Arg Thr Tyr Thr 380 Vai Lys Ala Leu 395 Giu Asp Leu Trp PCTIIBOO/00923 Ser Ser Asp Pro Gly Ala Giu Pro 400 Asn Asp 415 <210> 1105 <21i> 613 <212> DNA <213> Corynebacteriun giutanicum <220> <221> CDS <222> (101)..(613) <223> RXN3171 <400> 1105 atactttgtt ggactggaaa agtggccgtt tggttccctc caagcccaaa ttcgcccgcg cggtcttctt ctgggcggca atgatttaac atgtgaagct atg gac atc acc atc gtc Val cgc Arg atg Met acc Thr ccc Pro gcg Aia cgc aac Asn agc S er c tg Leu aaa Lys atc Ile ctg Leu gat cac His gac Asp atc Ile acc Thr atc Ile tcg Ser gag cca Pro aac Asn tac Tyr ccc Pro gtt Val1 atg Met gaa agc Ser cgt Arg cga Arg 45 gc t Ala cgt Arg gca Ala cca cgc Arg gca Ala 30 gat Asp gaa Giu gca Ala cag Gin gtc cta Leu gca Ala ctg Leu ggt Gly ggt Gly gtc Val cca acc Thr gcc Ala gaa Giu act Thr ctc Leu 80 ggc Gly tac Met

I

ctg Leu aac Asn gtC Val cgc Arg ggC Gly ttC Phe ctt Asp t tg Leu gac Asp gaa Giu ctg Leu atg Met att Ile gag Thr gac Asp ggC Gly ttC Phe cag Gin gac Asp ctt Leu 100 ctg Ile gag G iu gcc Ala.

gac Asp cca Pro cca Pro gcc Ala cca 1509 WO 01/00843 Arg Asp Giu cag gat cta Gin Asp Leu 120 acc ggc ggt Thr Gly Gly 135 gcc acc gac Ala Thr Asp 150 gac gca ttg Asp Ala Leu Thr His Giu Pro Val Pro 110 aac cag cct gta ttc ctt Asn Gin Pro Val Phe Leu 125 ctc ctg cac gcg atc cgc Leu Leu His Ala Ile Arg 140 acc gcc atc tgc atg gtt Thr Ala Ile Cys Met Val 155 gaa tct Giu Ser 170 Tyr gtc Val ctt Leu tct Ser 160 Leu Giu Ala LD 115 gat ccc atg ci Asp Pro Met Lo 130 ctt gct gat q Leu Ala Asp Ai 145 gcg cag cca g5 Ala Gin Pro G' PCTILBOOIOO923 bu Pro :g gcc 499 au Ala at ggc 547 c-g Gly it gtg 595 Ly Val 165 <210> 1106 <211> 171 <212> PRT <213> Corynebacterium giutanicuin <400> 1106 Met Asp Ile Thr 1 Leu Asn Val Arg Gly Phe Leu Asp Leu 145 Ala Leu Asp Giu Leu Met Ile Giu Pro 130 Ala Gin Ile Val Asn His Pro 5 Giu Arg Ser Asp Asn Ala Met Leu Ile Tyr Asp Thr Lys Thr Pro 55 Pro Pro Ile Ile Val 70 Pro Ala Leu Ser Met Ala Arg Asp Giu Giu 105 Pro Gin Asp Leu Ser 120 Ala Thr Giy Gly Ser 135 Gly Ala Thr Asp Ile 150 Val Asp Ala Leu Ala 165 Leu 10 Ala Giu Val Pro Ile 90 Thr Asn tLeu Thr Giu 170 Val Ala 'Ala Ala Ile 75 Pro His Gin Leu Ala 155 Ser Ala Ser Phe Arg Ser Arg Met Ala Ile Arg Asp Ala Giu Pro Pro Val 125 His Ala 140 Ile Cys Arg Leu Thr Ala Ala-Ala Asp Leu Giu Giu Gly Thr Ala Gly Leu Gin Val Gly Val Pro Tyr 110 Phe Leu Val Ile Arg Leu Met Val Ser 160 <210> 1107 1510 WO 01/00843 WO 0100843PCTIBOOIOO923 <211> <212> <213> <220> 613

DNA

Corynebacteriumf glut am ic ur <221> CDS <222> (101)..(613) <223> FRXA02857 <400> 1107 atactttgtt ggactggaaa agtggccgtt tggttccctc caagcccaaa ttcgcccgcg cggtcttctt ctgggcggca atgatttaac atgtgaagct atg gac atc acc Met Asp Ile Thr gtc aac cac cca ctc gtt gct agc cgc Val Asn His Pro Leu Val Ala Ser Arg cta Leu 15 acc ctg ttg cgc Thr Leu Leu Arg gac gag Asp Giu cgc agc gac Arg Ser Asp atg ctg atc Met Leu Ilie aac Asn gca gct ttc cgt Ala Ala Phe Arg gca gcc aac gac Ala Ala Asn Asp ctc ggc gcc Leu Gly Ala cac ttc gac His Phe Asp tac gaa gca tcc Tyr Glu Ala Ser gat ctg gaa gtc Asp Leu Glu Val gaa Giu acc aaa Thr Lys acc ccc gtt gcc Thr Pro Val Ala gct gaa ggt act Ala Giu Gly Thr ctg aag cag cca Leu Lys Gin Pro ccc Pro atc atc gtt ccc atc atc cgt gca ggt Ile Ile Val Pro Ile Ile Arg Ala Giy ggc atg atc gac Gly Met Ile Asp gcg ctg tcg atg Ala Leu Ser Met ccg gat gca cag Pro Asp Ala Gin ggc ttc att ggc Gly Phe Ile Gly ctt gcc Leu Ala 100 cgc gat gag Arg Asp Giu cag gat cta Gin Asp Leu 120 gaa Giu 105 acc cat gag cca Thr His Glu Pro cca tac ctt gag Pro Tyr Leu Glu gcg ctg cca Ala Leu Pro 115 atg ctg gc Met Leu Ala agc aac cag cct Ser Asn Gin Pro ttc ctt gtc gat Phe Leu Val Asp acc ggc Thr Gly 135 ggt tcc ctc ctg cac gcg atc cgc ctt Gly Ser Leu Leu His Ala Ile Arg Leu 140 gct gat cgt ggc Ala Asp Arg Gly gcc acc gac atc acc Ala Thr Asp Ilie Thr 150 gcc Ala 155 atc tgc atg gtt Ile Cys Met Val gcg cag cca ggt Ala Gin Pro Gly gtg Val1 165 gac gca ttg gcg Asp Ala Leu Ala gaa tct 613 Giu Ser 170 1511 WO 01/00843 <210> 1108 <211> 171 -<212> PRT <213> Corynebacterium glutamicum PCTIBOOIOO923 <400> 1108 Met Asp Ile Thr 1 Leu Asn Val1 Arg Gly Phe Leu Asp Leu 145 Ala Leu Asp Giu Leu Met Ile Giu Pro 130 Ala Gin Ile Val Asn His Pro 5 Giu Arg Ser Asp Asn Ala Met Leu Ile Tyr Asp Thr Lys Thr Pro Pro Pro Ile Ile Val 70 Pro Ala Leu Ser Met Ala Arg Asp Glu Glu 105 Pro Gin Asp Leu Ser 120 Ala Thr Gly Gly Ser 135 Gly Ala Thr Asp Ile 150 Leu 10 Ala Giu Val Pro Ile 90 Thr Asn Leu Thr Val Ala Ala Ala Ile 75 Pro His Gin Leu Ala 155 Arg Ala Asp Giu Ala Gin Val1 110 Phe Ile Met Leu Ala Leu Gly Gly Val Pro Leu Arg Val1 Thr Al a Giu Thr Leu Gly Tyr Val1 Leu Ser 160 Gly Val Asp Ala Leu Ala Giu Ser <210> 1109 <211> 424 <212> DNA <213> Corynebacterium glutamicun <22 0> <221> CDS <222> (101)..(424) <223> RXN00450 <400> 1109 tttgcgatga catggatttg gatccttccg aacaattgct gcgcatcgcg gaagaactcg gttttgataa tgatctggct cgggtggtgg gattcgacta gtg ggc gtt tta cct Val Gly Val Leu Pro 1 gtg cag gcg cgc atc aaa gac gat gag cgc cgc atg cgc cat gct ttg Val Gin Ala Arg Ile Lys Asp Asp Giu Arg Arg Met Arg His Ala Leu 15 115 163 1512 WO 01/00843 gat att gct cgc Asp Ile Ala Arg att tac gcg ccg Ilie Tyr Ala Pro gaa gca gac cgc Glu Ala Asp Arg cga gcc gcc cgc Arg Ala Ala Arg gcg gtg gtc acc Ala Val Val Thr ggt gct cga atc Gly Ala Arg Ile 105 caa acc cct Gin Thr Pro acc Thr gat Asp dgt Arg t tg Leu gga Gly ggg gag Gly Giu ccc acg Pro Thr 60 ttt tcc Phe Ser 75 gag ccc Glu Pro cgc atc Arg Ile ggg Gly 30 ctg Leu cac His ggc Gly agt Ser gcg Ala gcc Ala tgg Trp atg Met 95 gac gtt ccc gtt Asp Val Pro Val PCTIBOOIOO923 gcc gtc 211 Ala Val cgt cga 259 Arg Arg tta cga 307 Leu Arg tgc acc. 355 Cys Thr ttg gtg 403 Leu Val 100 <210> 1110 <211> 108 <212> PRT <213> Corynebacterium giutamicum <400> 1110 Val Gly Val Leu Pro Val Gin Ala Arg 1 Met Arg His Ala Leu Asp Ile Ala Arg Pro Val Gly Ala Val Ile Tyr Ala Pro Ala Thr Asn Arg Arg Glu Ala Asp Arg 55 Ile Ile Ala Leu Arg Arg Ala Ala Arg 70 Leu Ser Asp Cys Thr Ala Val Val Thr Ala Gly Ala Leu Val Gly Ala Arg Ile 100 105 <210> 1111 <211> 418 <212> DNA <213> Corynebacterium glutainicum <220> <221> CDS <222> (101)..(418) Asp Pro Glu Thr Ser Pro Ile Arg Val Thr Glu Arg Cys 1513 WO 01/00843 <223> FRXA00450 <400> 1111 tttgcgatga catggatttg gatccttccg aacaattgct gttttgataa tgatctggct cgggtggtgg gattcgacta PCT/EBOO/00923 gtg cag gcg cgc Val Gin Ala Arg gat Asp att Ile gaa Giu cga Arg gcg Aia ggt G iy gac Asp cct Pro gag Giu acg Thr tcc Ser ccc Pro gcgcatcgcg gaagaactcg gtg ggc gtt tta cct Val Gly Vai Leu Pro 1 atg cgc cat gct ttg 163 Met Arg His Aia Leu ccc gtt ggc gcc gtc 211 Pro Vai Gly Ala Val gca acg aac cgt cga 259 Ala Thr Asn Arg Arg att att gct tta cga 307 Ile Ile Ala Leu Arg ctg agt gac tgc acc 355 Leu Ser Asp Cys Thr gcc ggc gcc ttg gtg 403 Ala Gly Ala Leu Val 100 11i2 <2i1> 106 <212> PRT <213> Corynebacteriui giutanicuxn <400> 11i2 Vai Giy Val Leu Pro Val Gin Aia I 1 Met Arg His Ala Leu Asp Ile Ala 2 Pro Val Gly Ala Val Ile Tyr Ala 1 Ala Thr Asn Arg Arg Giu Ala Asp 1 55 Ile Ile Ala Leu Arg Arg Ala Ala 1 Leu Ser Asp Cys Thr Ala Val Val J Asp Pro Giu Thr Ser Pro 1514 WO 01/00843 WO 0100843PCTIIBOO/00923 Ala Gly Ala Leu Val Gly Ala Arg Ile Gly 100 105 <210> 1113 <211> 615 <212> DNA <213> Corynebacterium glut andcuin <220> <221> <222> <223>

CDS

(101)..(592) RX.A00465 <400> 1113 tccccaacgc gcaccgaact tagccggatc gcagacttca cctggatgtc caccgcagcc caagcgctac cagcgttgat gcgaggtttg agcgcctaac atg act gaa gat gac Met Thr Giu Asp Asp tta gat ctg ctg Leu Asp Leu Leu c ac His cgc aca gta gaa cta gcc acc cag gca Arg Thr Val Giu Leu Ala Thr Gin Ala 15 ctc aag Leu Lys cag gga aac agt cct tat gga tcc Gin'Gly Asn Ser Pro Tyr Gly Ser ctg gtt gat ccc Leu Val Asp Pro ttc ggc gcg Phe Gly Ala ctg acc aag Leu Thr*Lys gtc gtt ttt Val Val Phe gaa gac cac aac Giu Asp His Asn gat gcc gat ggg Asp Ala Asp Gly 259 cac ccg His Pro gaa ttc gcc atc gcc aaa tat gcg atc Glu Phe Ala Ile Ala Lys Tyr Ala Ile aat tac agt gca Asn Tyr Ser Ala tca Ser gaa cgt gct gcg Glu Arg Ala Ala tgc Cys 75 act gtt tat acc Thr Val Tyr Thr acg gaa cat tgc Thr Giu His Cys gcg Ala atg tgc gcc ggt Met Cys Ala Gly cat gcg tgg gct His Ala Trp Ala ctg ggc aaa att Leu Gly Lys Ile tac tgc Tyr Cys 100 355 403 451 499 gcc acc aca Ala Thr Thr gaa tct ggg Giu Ser Gly 120 ggg caa aca gcc Gly Gin Thr Ala tgg tac gca aag Trp Tyr Ala Lys tgg ggt gca Trp Gly Ala 115 agc ccg aac Ser Pro Asn cct ttg aac ccg Pro Leu Asn Pro att Ile 125 tca gcg gac aaa Ser Ala Asp Lys ata tcc Ile Ser 135 atc gaa gga cct Ile Giu Gly Pro gct Ala 140 tcc aga ttt gag gaa gtc ctg tat gaa Ser Arg Phe Giu Giu Val Leu Tyr Giu 145 ctg Leu 150 cat cga tgg ttt His Arg Trp Phe tat Tyr 155 tta ggg cag tct Leu Gly Gin Ser ccg Pro 160 aat aag gct ctt Asn Lys Ala Leu 1515 WO 01/00843 WO 0100843PCTIJSB00/00923 tagcgctggg catgtgactt taa <210> 1114 <211> 164 <212> PRT <213> Corynebacterium glutamicum <400> 1114 Met Thr Glu Asp Asp Leu Asp Leu Leu 615 1 Thr Asp Gly Glu Thr G ly Ala Lys Glu 145 Asn 5 Lys Ala Lys Ala Ala cys Ala Asn Glu Gly Val Pro Glu Cys Thr Ser Ser 135 His Asn Phe Glu Arg Ala Thr Gly 120 Ile Arg Ser Glu Phe Ala Gly Gly 105 Pro Glu Trp, His 10 Pro Asp Ala Ala Ala 90 Gly Leu Gly Phe Val Ser Arg Lys Val Trp Ala Ile 125 Ser Gly Glu Leu Asp Tyr Tyr Ala Ala 110 Ser Arg Gin Leu Leu Ala Ala Thr Gly Trp Ala Phe Ser Ala Val Asp Ile Ser Leu Tyr Asp Glu Pro 160 <210> 1115 <211> 1083 <212> DNA <213> Corynebacterium glutanicum <220> <221> CDS <222> (101)..(1060) <223> RXA00717 <400> 1115 aagcatcagt taaagccccg actattaaaa tctcctaaaa taggctagaa ttcacgggat tcaatttcat acgttttctc tcaagattaa ggacacttac gtg acc cca ccc gct 115 Val Thr Pro Pro Ala 1 1516 WO 01/00843 cgc cga gat Arg Arg Asp tac cgg tct Tyr Arg Ser aca cgc cag Thr Arg Gin aag cct gcc Lys Pro Ala ggt ttc tta Giy Phe Leu ctt gcc caa Leu Ala Gin gat cag ggc Asp Gin Gly gtg cgc gtg Val Arg Vai 120 atc cac atc Ilie His Ile 135 ggc atg cac Gly Met His 150 gat ctg gtc Asp Leu Val ggt cgc ctc Giy Arg Leu ggt gag ttg Giy Giu Leu 200 act tac ctt Thr Tyr Leu 215 gct ctt cgt Ala Leu Arg 230 gac Asp ggc Gly aag Lys aac Asn gac Asp gca Ala gtc Val aac Asn ctc Leu 140 agc Ser act Thr acc Thr ctc Leu cgc Arg 220 gag G iu aag Lys tac Tyr aag Lys gta Val1 tc t Ser tca Ser aac Asn gat Asp 125 gag Giu gat Asp gca Ala gaa Giu atg Met 205 ggt G iy ttg Leu aag Lys aag Lys gat Asp aaa Lys gac Asp cgt Arg gat Asp 110 gtc Val1 tac Tyr gaa Giu tct Ser ggt Giy 190 c ac His gaa Giu gaa Glu cag Gin cca Pro gag Giu tcc Ser gga Gly cga Arg cgt Arg atc Ile t tc Phe C tt Leu gga Gly 175 ttg Leu cc t Pro gc a Ala gat Asp agc Ser gga Gly att Ile gac Asp gtt Val1 80 cac His atc Ile cgt Arg gtg Val ggt Gly 160 cag Gin ctg Leu aag Lys acc Thr ggc Gly 240 tct Ser cgc Arg tcc Ser tgg Trp, cag Gin atc Ile acc Thr 115 9%c Gly aag Lys tgc Cys t tc Phe acc Thr 195 gtg Val cta Leu aag Lys PCTIBOO/00923 ggc gga 163 Gly Gly cca aac 211 Pro Asn aac gct 259 Asn Ala tcg atg 307 Ser Met aag gtg 355 Lys Val ctg att 403 Leu Ile 100 cag ggc 451 Gin Gly gtc cgc 499 Val*Arg cct cgt 547 Pro Arg gtg ggt 595 Val Gly 165 cac gtc 643 His Val 180 aac gat 691 Asn Asp tcc aag 739 Ser Lys gtc agc 787 Val Ser gct gac 835 Ala Asp 245 ttt gcg cag att atc gac gta ttc cag ggc aag tcc ttg ttg cgc atc 1517 WO 01/00843 Phe Ala Gi gaa atc ca Giu Ile Hi Ile gaa Glu 265 Phe cac His ctc ggt ttc cca gtc gag cgc ctc Leu Giy Phe Pro Vai Giu Arg Leu 280 285 cag ctt ggt gat cag aag cca ggtI 1027 Gin Leu Giy Asp Gin Lys Pro Giy 295 300 gag ctg acc agc tta tac aag gtg 1080 Glu Leu Thr Ser Leu Tyr Lys Val 310 315 cct 1083 <210> 1116 <211> 320 <212> PRT <213> Corynebacterium giutanicum 31n Itt Ile 270 Itg lai :cc 3er ;tc lai Gly 255 gtg Val cgc Arg ctt Leu caa Gin Thr 10 Val Pro Lys Arg Gly Val Pro Giu Thr Lys cga Arg ac c Thr cgt Arg c tg Leu 320 Pro Arg Gin Gin Asn 75 Val Glu Asn Asp Met 155 PCT/iBOO/00923 Ser Leu Leu Arg Ile 260 cgc ctc ttc gat gag 931 Arg Leu Phe Asp Giu 275 aag ctg cac acc gtt 979 Lys Leu His Thr Val 290 gca ctg aac tcc tct Ala Leu Asn Ser Ser 305 tgacggaaat ttccaacatg <400> 11i6 Val Thr Pro Pro Ala Arg Arg Asp Giy 1 Asn Ser Leu Asp Arg Ala Val Val1 Leu 145 Arg Ser Leu Asp Leu Glu Thr Asp 130 Asn Arg Arg Pro Phe Ala Gin Arg His 135 Met S er Gin Ala Leu Gin Gly Vai 120 Ile His Ser Gin Lys Asn Ala Arg 105 Asp Asn Ser Lys Tyr Lys Val1 Ser Ser Asn Asp 125 Giu Asp Gin Pro Giu Ser Gly Arg Arg Ile Phe Leu Ser Giy Ile Asp Vai His Ile Arg Val1 G ly 160 1518 WO 01/00843 Arg Pro Cys Arg Leu Phe Leu Leu Thr 195 Tyr Glu Val 210 Asn Lys Leu 225 Pro Ala Lys Ser Leu Leu Arg Leu Phe 275 Lys Leu His 290 Ala Leu Asn 305 Val His 180 Asn Ser Val Ala Arg 260 Asp Thr S er Asp Gly Gly Thr Ala 230 Phe Glu Leu Gin Glu 310 Thr Thr Leu Arg 220 Giu Val1 Lys Arg Pro 300 Lys PCT/iBOOIOO923 Ly Gin au Leu ro Lys La Thr 3p Gly 240 Ly Lys il Arg rg Thr L-u Arg Ln Leu 320 <210> 1117 <211> 978 <212> DNA <213> Corynebacterium glutanicun <220> <221> CDS <222> (101)..(955) <223> RXA01894 <400> 1117 agaatttttt cgaaaatgct ggcaccatca acagtgacat acccatgaat gaaccggagc aacatcaccg gtccatgagg aat aat gcg ggt cga gat ctc aaa Asn Asn Ala Gly Arg Asp Leu Lys ctg ggg gtc ctg gtt ctt ttg ggg Leu Gly Val Leu Val Leu Leu Gly tac atc ctc gtt gca ggt ttt atg Tyr Ile Leu Val Ala Gly Phe Met gct gcc att Ala Ala Ile 15 att gtc cta Ile Val Leu 30 gct gca gca Ala Ala Ala 1519 tgttagaaac t tcaaggaga atg ccc aaa ccc aaa Met Pro Lys Pro Lys 1 gct gtg ggg atc gga Ala Val Gly Ile Gly agc cca tgg ggt tgg Ser Pro Trp Gly Trp aca tgg gaa gtt ggt Thr Trp Glu Val Gly WO 01/00843 WO 0100843PCT/IBOO/00923 agc aga Ser Arg ctt aaa gaa ggc Leu Lys Glu Gly tat cat ttg cca ctg ccg att atg atc Tyr His Leu Pro Leu Pro Ile Met Ile atc Ile ggc ggt cag gca Gly Gly Gin Ala atc Ile 75 atc tgg ctg tca Ile Trp Leu Ser tgg Trp cca ttt ggc acg Pro Phe Gly Thr ggc att ttg gcg Gly Ile Leu Ala ttt gtg gcc act Phe Val Ala Thr ttg gtg ctg atg Leu Val Leu Met tat ttc Tyr Phe 100 cga att ttc Arg Ile Phe gac acc tct Asp Thr Ser 120 aat ggc acg gaa Asn Gly Thr Glu gaa gcc cgc aac Glu Ala Arg Asn tat ttg agg Tyr Leu Arg 115 ttg ttc gga Leu Phe Gly gtg ggc atc ttc Vai Gly Ile Phe gtg Val1 125 ctc acc tgg att Leu Thr Trp Ile cca Pro 130 agc ttc gct gcg atg ctg Ser Phe Ala Ala Met Leu 135 tcg Ser 140 ctg atg caa aac Leu Met Gin Asn aat Asn 145 tcc atc ccg ggt Ser Ile Pro Gly aca Thr 150 tat ttc att ttg Tyr Phe Ile Leu ttc atg ctg tgt Phe Met Leu Cys atc gca tcg gat Ile Ala Ser Asp gtg Val1 165 ggc ggg tat atc Gly Gly Tyr Ile ggt gtg ttc ttt Gly Val Phe Phe tcg cac cca atg Ser His Pro Met gcg ccg Ala Pro 180 ttg gtg agt Leu Vai Ser tta gga tcg Leu Giy Ser 200 aag aag tct tgg Lys Lys Ser Trp gaa Glu 190 ggc ttt gcc ggc Gly Phe Ala Gly tcc att gtc Ser Ile Val 195 ctc gat cac Leu Asp His gtc act ggt gca Val Thr Giy Ala agt gtt cac ttc Ser Val His Phe cac tgg His Trp 215 tgg atg ggt gtg Trp Met Gly Val ttg ggt tgt gcc Leu Gly Cys Ala gtt gtg tgc gcc Val Val Cys Ala acg Thr 230 ttg ggt gac ttg Leu Gly Asp Leu gag tcg cag ttc Giu Ser Gin Phe aaa Lys 240 cgc gat ttg ggc Arg Asp Leu Gly 787 835 883 aag gat atg tcg Lys Asp Met Ser ctt ctt cca ggc Leu Leu Pro Gly cac His 255 ggc gga ttg atg Gly Gly Leu Met gac cgt Asp Arg 260 ttg gat ggc Leu Asp Gly gtg atc agc Val Ile Ser 280 atg Met 265 ctc ccg gcc gcg Leu Pro Ala Ala gtg acg tgg ttg Vai Thr Trp Leu atc ctg agt Ile Leu Ser 275 agc tcg tat ccg Ser Ser Tyr Pro taaagcttgg gccagcttta agt 1520 WO 01/00843 WO 0100843PCTIIBOO/00923 <210> 1118 <211> 285 <212> PRT <213> Corynebacterium glutamicum <400> 1118 Met Pro Lys Pro Lys Asn Asn Ala Gly Arg Asp Leu Lys Ala Ala Ile 1 5 10 Ala Val Gly Ile Gly Leu Gly Val Leu Val Leu Leu Gly Ile Val Leu 25 Ser Pro Trp Gly Trp Tyr Ile Leu Val Ala Gly Phe Met Ala Ala Ala 40 Thr Trp Giu Val Gly Ser Arg Leu Lys Glu Gly Gly Tyr His Leu Pro 55 Leu Pro Ile Met Ile Ile Gly Gly Gin Ala Ile Ile Trp, Leu Ser Tip 70 75 Pro Phe Gly Thr Met Gly Ile Leu Ala Ser Phe Val Ala Thr Val Leu 90 Val Leu Met Tyr Phe Arg Ile Phe Tyr Asn Gly Thr Giu Lys Giu Ala 100 105 110 Arg Asn Tyr Leu Arg Asp Thr Ser Val Gly Ile Phe Val Leu Thr Tip, 115 120 125 Ile Pro Leu Phe Gly Ser Phe Ala Ala Met Leu Ser Leu Met Gin Asn 130 135 140 Asn Ser Ile Pro Gly Thr Tyr Phe Ile Leu Thr Phe Met Leu Cys Val 145 150 155 160 Ile Ala Ser Asp Val Gly Gly Tyr Ile Ala Gly Val Phe Phe Gly Ser 165 170 175 His Pro Met Ala Pro Leu Val Ser Pro Lys Lys Ser Tip Giu Gly Phe 180 185 190 Ala Gly Ser Ile Val Leu Gly Ser Val Thr Gly Ala Leu Ser Val His 195 200 205 Phe Leu Leu Asp His His Trp Trp Met Gly Val Ile Leu Gly Cys Ala 210 215 220 Leu Val Val Cys Aia Thr Leu Gly Asp Leu Val Giu Ser Gin Phe Lys 225 230 235 240 Arg Asp Leu Gly Ile Lys Asp Met Ser Asn Leu Leu Pro Gly His Gly 245 250 255 Gly Leu Met Asp Arg Leu Asp Gly Met Leu Pro Ala Ala Met Val Thr 260 265 270 Trp Leu Ile Leu Ser Val Ile Ser Ser Ser Tyr Pro Ser 275 280 285 1521 WO 01/00843 WO 0100843PCTIiBOOIOO923 <210> <211> <212> <213> <220> <221> <222> <223> 1119 879

DNA

Corynebacteriuni g iutaicum

CDS

(101) (856) RXA02536 <400> 1119 aagaagtgat cacgcgaacc tgtgtataac ttgcctcaaa gcgcctaggc tgtggattat gcgtattgcc ttgcttcaga tctcgacgaa ttccgataag atg gac aac ttc gcc Met Asp Asn Phe Ala ctg ctg cgt gat Leu Leu Arg Asp gct gaa aaa gct Ala Giu Lys Ala gaa cag ggg gct Giu Gin Gly Ala cgg gtg Arg Val ttg gtg ttt Leu Val Phe gat act cag Asp Thr Gin ccg Pro gag gcg act tcg Glu Ala Thr Ser agc ttt ggt acg Ser Phe Gly Thr gga agg ctt Gly Arg Leu gcg gta cga Ala Val Arg gcg gag gag ctc Ala Giu Giu Leu ggc gaa ttc tcc Gly Giu Phe Ser aaa tta Lys Leu gcc gat gag ctg Ala Asp Glu Leu gac Asp 60 git gtc atc gtt gcg ggc atg ttc acc Val Val Ile Val Ala Giy Met Phe Thr gct gac acc gtg Ala Asp Thr Val cgc ggt gaa. aaa Arg Giy Giu Lys atc tcg cgc gtc Ile Ser Arg Val aac acc gtg ctg Asn Thr Val Leu agt ggc gct gga Ser Gly Ala Gly cat cag gga tac His Gin Gly Tyr aac aaa Asn Lys 100 att cac aca Ile His Thr ccg ggc gat Pro Gly Asp 120 tat Tyr 105 gac gcg ttc ggt Asp Ala Phe Gly tat Tyr 110 agg gaa tcc gac Arg Giu Ser Asp act gtg aaa Thr Val Lys 115 aaa ttt ggt Lys Phe Gly gag ctg gtt gta Glu Leu Val Val t tc Phe 125 gag gtc gac gat Glu Val Asp Asp att Ile 130 gtg gcg Val Ala 135 aca tgc tac gat Thr Cys Tyr Asp att Ile 140 cga ttc cca Arg Phe Pro gaa cag Giu Gin 145 ttc aaa gac ctc Phe Lys Asp Leu cgc aac ggt gca Arg Asn Gly Ala ata att gtg gtt Ilie Ile Val Val acg tcg tgg caa Thr Ser Trp Gin ggt cct gga aaa tta gaa caa tgg gaa. gtc ctc cct cgc gcg cgt gca Gly Pro Gly Lys Leu Giu Gin Trp Giu Val Leu Pro Arg Ala Arg Ala 1522 WO 01/00843 WO 0100843PCT/IBOO/00923 tcc Ser t ta Leu 200 ac a Thr atg Met 175 gta gcg tgt ggg caa gcg cga Val Ala Cys Gly Gin Ala Arg 190 195 aaa ggc cct acg ggg att ggt Lys Gly Pro Thr Gly Ile Gly 205 210 gaa gta att gct-agc gcg ggt Giu Val Ile Ala Ser Ala Gly 225 att gat gtc agc ggt ttg gcc Ile Asp Val Ser Gly Leu Ala 240 taaccactgt ctaaggaatc act 180 ctt Leu cat His tat Tyr aaa Lys cca Pro tcc Ser gag Giu at t Ile 245 691 739 787 835 gag gca ttg Giu Ala Leu <210> 1120 <211> 252 <212> PRT <213> Corynebacterium giutamicum <400> 1120 Met Asp Asn Phe Ala Leu Leu Arg Asp 1 Gin Giy Ser Ala .le Gin Ser Asp Gin 145 Thr Pro Ala Asp Thr Leu Tyr 105 Glu Cys Gly Lys Ala 10 Giu Giu Giu Val1 Ile Asp Leu Tyr Ala Leu 170 Ala Gin Gly Val Giu Gly Tyr 110 Giu Phe Val Glu Ala Ser Giu Ile Lys Leu Arg Val1 Pro Val Val Giu Phe Phe Val1 Thr His Giu Asp Giu Pro 160 Leu 1523 WO 01/00843 WO 0100843PCT11B00100923 Pro Gin Gly Ser 225 Gly Ala Arg 195 Giy Giy Ala Arg 180 Leu His Tyr Lys Ala Leu Asp Ser Thr 185 Pro Giu Giu Leu Arg 200 Ser Met Val Thr Asn 215 Giu Pro Giu Met Leu 230 Ile Arg Giu Ala Leu 245 Trp Ile Val Ala Cys 190 Giu Arg Lys Gly Pro 205 His Gly Glu Val Ile 220 Ala Asp Ile Asp Val 235 Val Leu <210> 1121 <211> 1528 <212> DNA <213> Corynebacterlun glutamicum <22 0> <221> CDS <222> (101)..(1528) <223> RXN01209 <400> 1121 cagattgcag cacagaaggc atcggcgcca ggcagctttg cggtggcgtt tattgatgcg ctttatgacg tggatgccca ggctgtggcc tcgttggttg atg tgc gag agg cct Met Cys Giu Arg Pro gaa Giu ttg Leu tcc Ser gaa Glu cgc Arg ctg Leu gca Ala aag Lys ggt Gly ggc Gly gat Asp ggg Gly ggt Gly cgg Arg tac Tyr ggc Gly gga Gly gtt Val gtg Val ctt Leu gag Glu tat Tyr gga Gly cgc Arg ctg Leu tac Tyr ggc Gly gaa Glu ctg Leu att Ile gat Asp aaa Lys t ta Leu gat Asp ttg Leu 1 gtc Val gtt Val1 aag Lys gaa Giu gat Asp aca Thr gg t Gly 1524 WO 01/00843 WO 0100843PCT/iBOO/00923 aac ctg gat Asn Leu Asp 120 caa ttg cat gct gtg atc gcg cag tgc Gin Leu His Ala Val Ile Ala Gin Cys 125 gag act ggt Giu Thr Giy gtg gca Val Ala 135 ttg ccc gat gtg Leu Pro Asp Val att Ile 140 ggc att ggt ccg gtg gcc tct act gcg Giy Ile Gly Pro Val Ala Ser Thr Ala acc Thr 150 aaa cca gat gcg Lys Pro Asp Ala gca Ala 155 ccc gca ttg ggt gtg gag ggc atc gct Pro Ala Leu Gly Val Giu Gly Ile Ala 160 atc gcc gct gta Ile Ala Ala Val caa gac cac ggc Gin Asp His Gly gca tca gta gct Ala Ser Val Ala att gga Ile Gly 180 ggc gtt ggt Gly Val Gly ggt ctg tgc Gly Leu Cys 200 c ta Leu 185 cgc aac gcg gcc gaa ctc gct gct acg Arg Asn Ala Ala Giu Leu Ala Ala Thr 190 ccc atc gac Pro Ile Asp 195 cca gca gct Pro Ala Ala gtg gtc tct gaa Val Val Ser Glu atg acc gcc gcc Met Thr Ala Ala gcg gca act cgc ctg cgg Ala Ala Thr Arg Leu Arg 215 act Thr 220 gct ttt caa cct Ala Phe Gin Pro ttc tcg cct gaa Phe Ser Pro Giu act Thr 230 caa act gaa ctc Gin Thr Giu Leu tc t Ser 235 caa aca gaa ctc Gin Thr Giu Leu caa Gin 240 gga gcc ttc gtg Gly Ala Phe Val tcg cct tct gcc Ser Pro Ser Ala cgt gtg ttg tct att gca ggc act gat Arg Val Leu Ser Ile Ala Gly Thr Asp 255 ccc aca Pro Thr 260 ggt ggt gca ggt att cag gct gat ctg Gly Gly Ala Gly Ile Gin Ala Asp Leu 265 270 aag tcc att gca Lys Ser Ile Ala gca ggt ggc Ala Gly Gly' 275 aac acc cac Asn Thr His ggc tac ggc Gly Tyr Gly 280 atg tgc gtt gtg Met Cys Val Val tcg ctg gtc gcg Ser Leu Val Ala ggC gtc aac acg atc cac acc cca ccc ttg acc ttt ttg gaa gaa cag 1027 Gly Val 295 ctg gaa 1075 Leu Giu 310 atg ttg 1123 Met Leu Asn Thr Ile His Pro Pro Leu Thr Leu Giu Giu Gin gcg gtc ttt tcc gat gtc acc gtc gat gcc atc aag ctc ggc Ala Val Phe Ser 315 Asp Val Thr Val Asp 320 Ala Ile Lys Leu Gly 325 ggc tct gcc gac acc gtc gat ctg gtg gct tca tgg ctt ggt Gly Ser Ala 330 Asp Thr Val Asp Leu Val Ala Ser Trp 335 Leu Gly 340 1525 WO 01/00843 tcc cac ga 1171 Ser His G1I agc ggt ga 1219 Ser Gly Asj 361 ctg gcc gt5 1267 Leu Ala Va: 375 gtg ttg tg 1315 Val Leu Cy 390 cag gct ca 1363 Gin Ala G1i ggt gga cal 1411 Gly Gly Hi! gac ggc tcc 1459 Asp Gly Set 441 tcc cat gge 1507 Ser His Gl1 455 gcc gcc gg( 1528 Ala Ala G1l 470 <210> 1122 <211> 476 PCT/IBOO/00923 1 cac .i His 345 t cgc p Arg ggt ccc gtg gtg ctt Gly Pro Val Val Leu 350 cta ctg gat gcg agc Leu Leu Asp Ala Ser 365 gtc gat gtg gtc acc Val Asp Val Val Thr gat ccc gtc atg atc gcc acc Asp Pro Val Met Ile Ala Thr 355 gct gaa gaa tcg ctg cgc cgc Ala Glu Glu Ser Leu Arg Arg 370 ccg aat atc ccc gaa ctt gcc Pro Asn Ile Pro Giu Leu Ala cac His 380 385 c gac agt gct s Asp Ser Ala 395 g gga ttt gcg i Gly Phe Ala cct gcc atc acc atg Pro Ala Ile Thr Met 400 cgg act cat gac acc Arg Thr His Asp Thr 415 gcg ctt gct gat aac Ala Leu Ala Asp Asn gat Asp gag gcc att gct Glu Ala Ile Ala 405 t ctg s Leu 425 g gtg r Val 410 act ggc Thr Gly atc gtc att gtc aag Ile Val Ile Val Lys 420 gct gtc gtg cgc ccc Ala Val Val Arg Pro 435 ttc cag gtg gaa Phe Gin Val Glu 430 aac ctg cgt gtc aac acc acc aac Asn Leu Arg Vai Asn Thr Thr Asn 450 tct gcg tca ctt gcc acc aag atc Ser Ala Ser Leu Ala Thr Lys Ile 465 c aca ggc tgt tcg I Thr Giy Cys Ser 460 c gaa agc gtg gaa Glu Ser Val Glu 475 <212> PRT <213> Corynebacterium giutamicum <400> 1122 Met Cys Glu Arg Pro Glu Lys Tyr Val 1 5 Vai Thr Asp Pro Val Leu Gly Giy Gly 25 Val Asp Ser Ala Ile Ser Giy Gly Val 40 Thr Asp Phe Ser Leu Tyr Leu 10 Pro Lys Lys Val Aia Gly Ile Ser Vai Val Gln Leu Arg Asp 1526 WO 01/00843 Lys Asn Ser Glu Leu Cys Asp Ile Ala Thr Pro Tyr Gly Leu Ser 115 Cys Ala Glu 130 Val Ala Ser 145 Glu Gly Ile Ser Val Ala Ala Thr Pro 195 Ala Asn Pro 210 Thr Phe Ser 225 Gly Ala Phe Gly Thr Asp Ile Ala Ala 275 Ala Gin Asn 290 Phe Leu Glu 305 Ala Ile Lys Ala Ser Trp Val Met Ile 355 Gly Asp Val1 Thr 100 Ile Thr Thr Ala Ile 180 Ile Ala Pro Val Pro 260 Gly Thr Glu Leu Leu 340 Ala Val Val1 Leu Glu Asp 120 Leu Pro Ala Gly Cys 200 Thr Thr Ser Ala Gly 280 Asn Ala Gly Glu Asp 360 Arg Ala His Leu 105 Gin Pro Asp Val Leu 185 Val1 Arg Glu Ala Gly 265 Met Thr Val1 Ser His 345 Arg Ala Leu Leu Leu Leu Asp Ala Ala 170 Arg Val1 Leu Leu Pro 250 Ile Cys Ile Phe Ala 330 Gly Leu Ala Val 75 His Pro His Val1 Ala 155 Gin Asn Ser Arg Ser 235 Arg Gin Val1 His Ser 315 Asp Pro Leu Glu Asp Gin Leu 110 Ile Ile Leu Gly Glu 190 Met Phe Glu Ser Leu 270 Ser Pro Thr Asp Leu 350 Ser PCTLBOO/00923 Leu Lys Tyr Leu Gly Asp Glu Leu Ala Gin Gly Pro Gly Val 160 Ile Ala 175 Leu Ala Thr Ala Gin Pro Leu Gin 240 Ile Ala 255 Lys Ser Leu Val Leu Thr Val Asp 320 Leu Val 335 Asp Pro Ala Glu Glu Ser Leu Arg Arg Leu Ala Val His Val Asp Val Val Thr Pro Asn 1527 WO 01/00843 370 375 Ile Pro Giu Leu Ala Vai Leu Cys Asp Ser Ala 385 390 395 Asp Giu Ala Ile Ala Gin Ala Gin Gly Phe Ala 405 410 Ile Val Ile Val Lys Gly Gly His Leu Thr Gly 420 425 Ala Val Val Arg Pro Asp Gly Ser Val Phe Gin 435 440 Vai Asn Thr Thr Asn Ser His Giy Thr Giy Cys 450 455 Leu Ala Thr Lys Ile Ala Ala Gly Glu Ser Val 465 470 475 <210> 1123 <211> 1528 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (10i)...(i528) <223> FRXA01209 <400> 1123 cagattgcag cacagaaggc atcggcgcca ggcagctttg ctttatgacg tggatgccca ggctgtggcc tcgttggttg PCT/EBOO/00923 380 Pro Ala Ile Thr Arg Thr His Asp 415 Ala Leu Ala Asp 430 Val Giu Asn Leu 445 Ser Leu Ser Ala 460 Glu gaa Giu ttg Leu tcc Ser gaa Giu cgc Arg c tg Leu aag Lys ggt Gly ggC Gly gat Asp ggg Gly ggt Gly ga t Asp aaa Lys gtg Val gca Ala gtt Val 75 cac His ttt Phe aaa Lys gtg Val1 gca Ala 60 gtc Val1 att Ile ttg Leu gct Ala ctg Leu gag Giu gat Asp caa Gin tat Tyr gga Gly cgc Arg ctg Leu tac Tyr ggC Gly cggtggcgtt tattgatgcg atg tgc gag agg cct Met Cys Giu Arg Pro 1 gtc acc gat ccc gtt Val Thr Asp Pro Val gtt gac agc gca att Val Asp Ser Ala Ile aag aac tca ggc gtg Lys Asn Ser Gly Val gaa ctc tgc gat gct Glu Leu Cys Asp Ala gat atc gcc gtt gag Asp Ile Ala Val Glu aca cct tat acg caa Thr Pro Tyr Thr Gin 100 115 163 211 259 307 355 403 1528 WO 01/00843 WO 0100843PCT/BOOIOO923 gca cgg Ala Arg aac ctg Asn Leu gtg gca Vai Ala 135 acc aaa Thr Lys 150 atc gcc Ile Ala ggc gtt Gly Vai ggt ctg Gly Leu gcg gca Ala Ala .215 act caa Thr Gin 230 tcg cct Ser Pro ggt ggt Gly Gly ggc tac Giy Tyr ggc gtc 1027 Gly Vai 295 ctg gaa 1075 Leu Giu 310 atg ttg 1123 gag Giu gat Asp 120 ttg Leu cca Pro gc t Ala ggt Giy tgc Cys 200 act Thr act Thr tc t Ser gca Ala ggc Gly 280 aac Asn gcg Ala ggc ctg Leu 105 caa Gin ccc Pro gat Asp gta Val cta Leu 185 gtg Val cgc Arg gaa Glu gcc Ala ggt Gly 265 atg Met acg Thr gtc Val tct ctt Leu t tg Leu gat Asp gcg Ala gct Ala 170 cgc Arg gtc Val ctg Leu ctc Leu cca Pro 250 att Ile tgc Cys atc Ile ttt Phe gc cca Pro cat His gtg Vai gca Al a 155 caa Gin aac Asn tct Ser cgg Arg tc t Ser 235 cgt Arg cag Gin gt t Vai c ac His t cc Ser 315 gac gct Ala gct Ala att Ile 140 ccc Pro gac Asp gcg Ala gaa Glu act Thr 220 caa Gin gtg Val1 gct Ala gtg Val1 ac c Thr 300 gat Asp acc cat His gtg Val1 125 ggc Gly gca Ala cac His gcc Ala atc Ile 205 gct Ala aca Thr ttg Leu gat Asp acc Thr 285 cca Pro gtc Val gtc ctt Leu 110 atc Ile att Ile ttg Leu ggc Gly gaa Glu 190 atg Met ttt Phe gaa Giu t ct Ser ctg Leu 270 tcg Ser ccc Pro acc Thr gat gaa Glu gcg Ala ggt Gly ggt Gly atc Ile 175 ctc Leu acc Thr .c aa Gin ctc Leu att Ile 255 aag Lys ctg Leu ttg Leu gtc Val ctg ttg Leu cag Gin ccg Pro gtg Val 160 gca Ala gct Ala gcc Ala cct Pro caa Gin 240 gca Ala tcc Ser gtc Val acc Thr gat Asp 320 gtg ggt Gly tgc Cys gtg Val 145 gag Giu tca Ser gc t Ala gcc Ala act Thr 225 gga Gly 9gc Gly at t Ile gcg Ala ttt Phe 305 gcc Ala gct t tg Leu gcc Ala 130 gcc Ala ggc Gly gta Val acg Thr aat Asn 210 ttc Phe gcc Ala act Thr gca Ala caa Gin 290 ttg Leu atc Ile tca agc Ser 115 gag Giu tct Ser atc Ile gc t Ala ccc Pro 195 cca Pro tcg Ser ttc Phe gat Asp gca Ala 275 aac Asn gaa Giu aag Lys tgg att Ile act Thr act Thr gct Ala att Ile 180 atc Ile gca Ala cct Pro gtg Val ccc Pro 260 ggt Gly acc Thr gaa Giu ctc Leu c tt gaa Glu ggt Gly gcg Ala gag Giu 165 gga Gly gac Asp gct Aia gaa Giu aa t Asn 245 aca Thr ggc Gly cac His cag Gin 9gc Gly 325 ggt 1529 WO 01/00843 PCTIBOOIOO923 Met Leu Gly Ser Ala Asp Thr Val Asp Leu Vai Ala Ser Trp Leu Giy 330 335 340 tcc cac gag cac ggt ccc gtg gtg ctt gat ccc gtc atg atc gcc acc 1171 Ser His Giu His Giy Pro Val Val Leu Asp Pro Val Met Ile Ala Thr 345 350 355 agc ggt gat cgc cta ctg gat gcg agc gct gaa gaa tcg ctg cgc cgc 1219 Ser Gly Asp Arg Leu Leu Asp Ala Ser Ala Giu Glu Ser Leu Arg Arg 360 365 370 ctg gcc gtg cac gtc gat gtg gtc acc ccg aat atc ccc gaa ctt gcc 1267 Leu Ala Val His Vai Asp Val Val Thr Pro Asn Ile Pro Giu Leu Ala 375 380 385 gtg ttg tgc gac agt gct cct gcc atc acc atg gat gag gcc att gct 1315 Val Leu Cys Asp Ser Ala Pro Ala Ile Thr Met Asp Giu Ala Ile Ala 390 395 400 405 cag gct cag gga ttt gcg cgg act cat gac acc atc gtc att gtc aag 1363 Gin Ala Gin Gly Phe Ala Arg Thr His Asp Thr Ile Val Ile Val Lys 410 415 420 ggt gga cat ctg act ggc gcg ctt gct gat aac gct gtc gtg cgc ccc.

1411 Giy Giy His Leu Thr Gly Ala Leu Ala Asp Asn Ala Val Val Arg Pro 425 430 435 gac ggc tcg gtg ttc cag gtg gaa aac ctg cgt gtc aac acc acc aac 1459 Asp Gly Ser Val Phe Gin Val Giu Asn Leu Arg Vai Asn Thr Thr Asn 440 445 450 tcc cat ggc aca ggc tgt tcg ctc tct gcg tca ctt gcc acc aag atc 1507 Ser His Gly Thr Gly Cys Ser Leu Ser Ala Ser Leu Ala Thr Lys Ile 455 460 465 gcc gcc ggc gaa agc gtg gaa 1528 Ala Ala Gly Glu Ser Val Giu 470 475 <210> 1124 <211> 476 <212> PRT <213> Corynebacterium giutanicun <400> 1124 Met Cys Giu Arg Pro Giu Lys Tyr Val Thr Asp Phe Ser Leu Tyr Leu 1 5 10 Val Thr Asp Pro Val Leu Gly Gly Gly Pro Lys Lys Val Ala Gly Ile 25 1530 WO 01/00843 Val Asp Ser Lys Asn Ser Giu Leu Cys Asp Ile Ala Thr Pro Tyr Gly Leu Ser 115 Cys Ala Giu 130 Val Ala Ser 145 Giu Gly Ile Ser Val Ala Ala Thr Pro 195 Ala Asn Pro 210 Thr Phe Ser 225 Gly Ala Phe Gly Thr Asp Ile Ala Ala 275 Ala Gin Asn 290 Phe Leu Glu 305 Ala Ile Lys Ala Ser Tip PCTAIBOOIOO923 Ala Gly Asp Val1 Thr 100 Ile Thr Thr Ala Ile 180 Ile Ala Pro Val Pro 260 Gly Thr Giu Leu Leu 340 Ile Val Ala Giu Gin Giu Gly Ala Giu 165 G ly Asp Ala Glu Asn 245 Thr Gly His Gin Gly 325 Gly Ser Giu Arg 70 Leu Ala Asn Val Thr 150 Ile Gly Gly Ala Thr 230 Ser Gly Gly Gly Leu 310 Met Ser Gly Asp 55 Gly Gly Arg Leu Ala 135 Lys Ala Val Leu Ala 215 Gin Pro Gly Tyr Val 295 Giu Leu His G ly 40 Val Val Leu Giu Asp 120 Leu Pro Ala Gly Cys 200 Thr Thr Ser Ala Gly 280 Asn Ala Gly Glu Val Arg Ala His Leu 105 Gin Pro Asp Val Leu 185 Val Arg Glu Ala Gly 265 Met Thr Val1 Ser His 345 Ser Ala Leu Leu 90 Leu Leu Asp Ala Ala 170 Arg Val Leu Leu Pro 250 Ile Cys Ile Phe Ala 330 Gly Val Ala Val1 75 His Pro His Val1 Ala 155 Gin Asn Ser Arg Ser 235 Arg Gin Val His Ser 315 Asp Pro Val Ala Val Ile Ala Ala Ile 140 Pro Asp Ala Glu Thr 220 Gin Val1 Ala Val1 Thr 300 Asp Thr Val1 Gin Leu Lys Giu Asn Asp Gly Gin His Leu 110 Val Ile 125 Gly Ile Ala Leu His Gly Ala Giu 190 Ile Met 205 Ala Phe Thr Giu Leu Ser Asp Leu 270 Thr Ser 285 Pro Pro Val Thr Val Asp Val Leu 350 Arg Leu Tyr Gly Giu Ala Gly Gly Ile 175 Leu Thr Gin Leu Ile 255 Lys Leu Leu Val Leu 335 Asp Asp Lys Leu Asp Leu Gin Pro Val 160 Ala Ala Ala Pro Gin 240 Ala Ser Val Thr Asp 320 Val Pro Val Met Ile Ala Thr Ser Gly Asp Arg Leu Leu Asp Ala Ser Ala Giu 1531 WO 01/00843 WO 0100843PCTIIBOO/00923 355 360 Giu Ser Leu Arg Arg Leu Ala Val His Val Asp 370 375 Ile Pro Giu Leu Ala Val Leu Cys Asp Ser Ala 385 390 395 Asp Giu Ala Ile Ala Gin Ala Gin Gly Phe Ala 405 410 Ile Val Ile Val Lys Gly Gly His Leu Thr Gly 420 425 Ala Val Val Arg Pro Asp Gly Ser Vai Phe Gin 435 440 Vai Asn Thr Thr Asn Ser His Gly Thr Gly Cys 450 455 Leu Ala Thr Lys Ile Ala Ala Gly Glu Ser Vai 465 470 475 <210> 1125 <211> 795 <212> DNA <213> Corynebacteriun giutanicum <220> <221> CDS <222> (101)..(772) <223> RXN01617 <400> 1125 tcagaagcta ccggcggcgc cggcatccag gttgatttga tgtttatggc attgggtcca tcacatgctt gggtggcctt Val 380 Pro Arg Al a Val Ser 460 Giu Thr Ile His Ala 430 Asn Ser gga Gly att Ilie a tg Met gaa Glu ggc G iy acc Thr gcc Al a ggt Giy agc Ser tcC Ser gca Aia gca Aia cac His 60 gcg Ala cgg ttt Arg Phe gcg cac Ala His 30 acg atc Thr Ile gtt gtc Val Val gca ctt Ala Leu gat Asp gat Asp gat Asp cta Leu gat Asp agaccttcca gcatttaaga ttg atc cta aag aca Leu Ile Leu Lys Thr 1 cag gtt atc gct aat Gin Vai Ile Ala Asn gat gtg gtg aag atc Asp Val Val Lys Ile gtg gca acc gct ttg Val Ala Thr Ala Leu ccg gta ctg atc tgc Pro Val Leu Ile Cys gac act gcc ctt cgc Asp Thr Ala Leu Arg cag gag ccc Gin Glu Pro 1532 WO 01/00843 gcg aag gtg Ala Lys Val gcc acc acc Ala Thr Thr aag gaa gca Lys Glu Ala 120 gtt aag ggt Val Lys Gly 135 ttc gac ggc Phe Asp Gly 150 gag cgc gtc Glu Arg Val gag ctg gcc Glu Leu Ala cgc gta gtc Arg Val Val 200 PCT/LB00100923 ctg Leu ctg Leu 105 gcc Ala 9gC Gly acc Thr tcc Ser aag Lys 185 acc Thr gtg Val aag Lys 110 gag Glu ggc Gly ttc Phe acc Thr gtt Val1 190 gac Asp gt t Val 95 ctg Leu caa Gln gac Asp tct Ser t tc Phe 175 gat Asp gct Ala act Thr gag Glu gga Gly aac Asn gaa Glu 160 gca Ala gca Ala gtc Val1 ttc Phe 100 gac Asp gtc Val1 gta Val1 Gly acc Thr 180 gc t Ala gca gag Glu ctg Leu gtt Val ctt Leu gac Asp 165 gca Ala aag Lys ccg 403 451 499 547 595 643 691 739 Ala Ser Asn Ala Pro 210 ttt acc Phe Thr 215 tct gtg tgg ttg Ser Val Trp Leu gaa gac aac aag Glu Asp Asn Lys tagaaatctt aaacaagctc 792 cc t <210> 1126 <211> 224 <212> PRT <213> Corynebacterium glutainicum <400> 1126 Leu Ile Leu Lys Thr Thr Giy Ile ThrI 1 Gin Val Ile Ala Asn Gln Ile Giu Alaj 25 Asp Val Vai Lys Ile Gly Met Leu Gly Val Ala Thr Ala ILeu Glu Glu Asn Ser1 Pro Val Leu Ile Cys Lys Gly Gin Glu 1 Asp Thr Ala Leu Arg Ala Lys Val Leu 1 1533 Phe His Ile Val1 Leu Val WO 01/00843 Asn Ile Gin 130 Val Lys Val Thr Ser 210 Asn Asp 115 Tyr Asp Ile Ile Thr 195 Asn Thr Ala 120 Gly Gly Val Ala Val 200 Ser <210> 1127 <211> 638 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> <223> FRXA01617 <400> 1127 gct aat cag att gag gcc gcc acc Ala Asn Gin Ile Giu Ala Ala Thr 1 5 aag atc ggt atg ttg ggt act cct Lys Ile Gly Met Leu Gly Thr Pro gct ttg gag gaa aac agc ttc aag Ala Leu Giu Giu Asn Ser Phe Lys atc tgc aag ggc cag gag ccc ggc Ile Cys Lys Giy Gin Glu Pro Gly 55 ctt cgc gcg aag gtg ctg cca cag Leu Arg Ala Lys Val Leu Pro Gin 70 Leu 105 Al1a Gly Thr Ser Lys 185 rhr Val1 gca Ala gca kla cac gcg Nla gca kla Ser Arg Ile Asp G ly 170 G ly Arg Trp gcg Ala acg Thr gtt Val1 gca Al a ac c Thr Gly Leu Asp Tyr 155 Ala Asn Ala Leu cac His atc I le gtc Val ctt Leu gtg Val Leu Ile Phe 140 His Gly Ser Val Ala 220 gat Asp gat Asp cta Leu gat Asp gtt Val PCTIIBOO/00923 Leu Giu Gln Gly Asp Asn Ser Giu 160 Phe Ala 175 Asp Ala Ala Val Asn Lys 1534 WO 01/00843 ttc gag gcc Phe Glu Ala gac ctg aag Asp Leu Lys gtc gtt gtt Val Val Val 115 gta ctt ttc Val Leu Phe 130 ggC gac gag Gly Asp Glu 145 acc gca gag Thr Ala Glu gct aag cgc Ala Lys Arg gca ccg ttt Ala Pro Phe 195 acc acc ctg Thr Thr Leu gaa gca gcc Giu Ala Ala 100 aag ggt ggc Lys Gly Gly gac ggc acc Asp Gly Thr cgc gtc tcc Arg Val Ser 150 ctg gcc aag Leu Ala Lys 165 gta gtc acc Val Val Thr 180 acc tct gtg Thr Ser Val tct Ser cgc Arg atc Ile gac Asp 135 ggc Gly ggc Gly cgc Arg ggc Gly ctc Leu gac Asp 120 tac Tyr gct Ala aac Asn gca Ala acc Thr cc t Pro 110 gc t Ala cca Pro gc t Ala gtg Val gca Ala 190 PCTIBOOIOO923 atc gac 288 Ile Asp cag tac 336 Gln Tyr gtg gac 384 Val Asp aag atc 432 Lys Ile gtc atc 480 Val Ile 160 acc acc 528 Thr Thr 175 tcc aac 576 Ser Asn tgg ttg gcg gaa gac aac aag tagaaatctt Trp Leu Ala Glu Asp Asn Lys 200 205 aaacaagctc cct <210> 1128 <211> 205 <212> PRT <213> Corynebacterium giutanicum <400> 1128 Ala Asn Gln Ile Glu Ala Ala Thr I 1 5 Lys Ile Giy Met Leu Gly Thr Pro Ala Leu Glu Glu Asn Ser Phe Lys F Ile Cys Lys Giy Gln Glu Pro Gly Leu Arg Ala Lys Val Leu Pro Gin 7 Phe Giu Ala Thr Thr Leu Ser Gly I Asp Leu Lys Giu Ala Ala Arg Leu I 1001 ~la la is l1a la ~eu I :le 1535 kla 10 ['hr fal kla ['hr ~sp 90 fi s Val1 Ala Val Thr Asn Ile Gin WO 01/00843 WO 0100843PCTIBOO/00923 Val Val Val Lys Gly Gly Ilie Asp Phe Pro Gly 115 120 Val Leu Phe Asp Gly Thr Asp Tyr His Val Phe 130 135 Gly Asp Glu Arg Val Ser Gly Ala Gly Cys Thr 145 150 155 Thr Ala Glu Leu Ala Lys Gly Asn Ser Ala Val 165 170 Ala Lys Arg Val Val Thr Arg Ala Val Lys Asp 180 185 Ala Pro Phe Thr Ser Val Trp Leu Ala Giu Asp 195 200 <210> 1129 <211> 792 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (769) <223> RXCO1600 <400> 1129 tgagtacaaa tctcgtccaa cccatgctca tccactgttt tttggagctg cgtgtccacc cttagatcta caatgtgatc Asn 125 Glu Ala Ala Val1 Lys 205 Ala Val Pro Lys Ala Val Val Thr 175 Ala Ser 190 cac His t ta Leu gg t Gly gca Ala cgt Arg gat Asp att Ile gag Glu ggt Gly gtg Val1 cgc Arg att Ile ccc Pro tcc Ser tcc Ser gtt Val agc Ser gc t Ala gag Glu ggc Gly cgt Arg gg t Gly 60 tcc Ser gaa Glu ttc Phe gtt Val gaa Glu 45 gaa Giu ttg Leu cc Leu tacggcctgg tgaagaccgc atg gtt tcg aag atg Met Val Ser Lys Met 1 gat act gaa ctg ttg Asp Thr Glu Leu Leu tca ttg atc atg ccg Ser Leu Ile Met Pro cac ttt ggg gcg gtc His Phe Gly Ala Val atg gtc aag cag tac Met Val Lys Gin Tyr cct gca ggt ttg ttg Pro Ala Gly Leu Leu cag cgc gag ctc atg Gin Arg Giu Leu Met 100 1536 WO 01/00843 gag gag gct ggt ttg gag gcc agt Glu Glu Ala Gly Leu Glu Ala SerC 105 att acc tcg cct ggt ttc tgc gat 5 Ile Thr Ser Pro Gly Phe Cys Asp 120 125 cga ggc ctc aca aag gtt gag cgc Arg Gly Leu Thr Lys Val Glu Arg 1 135 140 gcg gac atg att aac cag tgg gtt Ala Asp Met Ile Asn Gin Trp Val 1 150 155 gtg ttt agt ggc cag ttg gtt aacI Val Phe Ser Giy Gin Leu Val Asn 170 gct gct gat gct gtg att gcg ggtc Ala Ala Asp Ala Val Ile Ala Gly 2 185 gcg ccg ttt acc tat cgc cct acg5 Ala Pro Phe Thr Tyr Arg Pro Thr 2 200 205 cac ggc att gtt cct gac atg aaa His Gly Ile Val Pro Asp Met Lys 215 220 aaa <210> 1130 <211> 223 <212> PRT <213> Corynebacterium glutamicum <400> 1130 Met Val Ser Lys Met His Ile Pro 1 Asp Thr Giu Leu Leu Leu Glu Ser Ser Leu Ilie Met Pro Giy Gly Ser 40 His Phe Gly Ala Val Ala Val Val2 Met Val Lys Gin Tyr Arg Arg Ser Pro Ala Gly Leu Leu Asp Ile Ala Gin Arg Glu Leu Met Glu Giu Ala 100 ctc Leu gtc Val1 130 ggc Gly gca Ala gcg Ala cgt Arg cgt act Thr 115 ttt Phe ga t Asp gtg Val1 gg t Gly gcc Al a 195 cga PCTIBOOIOO923 at ttg 451 sp Leu ta gcc 499 eu Ala aa gaa 547 lu Giu ga atg 595 ly Met 165 tc atg 643 al Met tc acc 691 al Thr aa gcg 739 Ala Gin Arg Arg Lys Ala 210 tgaaggctcg cgttttagcg iaa cta y Leu 3fly Pro 25 ['hr I kla I J'al( ksp fly I 1537 Phe Val1 Giu Glu Leu Leu Ser Thr Arg Val1 Asn Trp Thr Giu 110 WO 01/00843 WO 0100843PCTIiBOO/00923 Val Arg Met 145 Giu Ile Ala Gin Leu Val i3 0 Giy Aia Ala Arg Arg 210 Thr 115 Phe Asp Val1 Gly Aia 195 Arg Thr Giy 135 Asp Phe Ala Pro Gly 215 Cys Giu 140 Trp Val Ala Pro Met 220 Asp 125 Arg Val Asn Gly Thr 205 Lys <210> 1131 <211> 726 <212> DNA <213> Corynebacteriun giutamicum <22 0> <221> CDS <222> (101)..(703) <223> RXC01622 <400> 1131 aaggcgtggg cgtttttgaa ggagcttcgt ttggagcgcg gccatcgcag agctgaagag ctggtgggaa ggagaaaaca gcc Ala atg Met agc Ser aac Asn tgg Trp ttg gac agg Asp Arg ttg ttg Leu Leu atc gtg Ile Val att tct Ilie Ser gtg gac Val Asp agc cag ttg Leu gtg Val1 ttg Leu tca Ser ctc Leu cag ttt Phe gct Ala a tc Ile cgt Arg acc Thr gct aac gcg atg gag Asn Ala Met Glu gcg ccc gat Ala Pro Asp atg Met 30 tc t Ser gct Ala gtcctttgga tcgtgaagtt atg agt gat ttt tat Met Ser Asp Phe Tyr 1 gag gta gct cca ggc Glu Val Ala Pro Gly gag gat ttt gag cgc Giu Asp Phe Glu Arg acc act ttt ggt gtg Thr Thr Phe Gly Val aat gtg ttg ccc gag Asn Val Leu Pro Giu tac atc ggt ggg ccg Tyr Ile Gly Gly Pro acc aag ccg ggc gtg cag gca Gin Ala ttg ggc 1538 WO 01/00843 Leu Ser Gin Gin Ala Val Val Gly L gat att gaa aat tcc acc agc ttc a Asp Ilie Glu Asn Ser Thr Ser Phe 105 1 cac gtg gat ctg cgt tct gca cct q His Val Asp Leu Arg Ser Ala Pro C 120 125 ggc atg cgc ttt ttt gcg ggc tac C Gly Met Arg Phe Phe Ala Gly Tyr .1 135 140 aac gag gaa att gag cag ggt gat t Asn Giu Giu Ile Giu Gin Gly Asp 9 150 155 tcg gac att atc gcg ccg ggc cgc Ser Asp Ile Ile Ala Pro Gly Arg N~ 170 cgt cga caa gca atg cct ttg ccgt Arg Arg Gin Ala Met Pro Leu Pro I 185 cct tca gat aat tagatgagtt ccgaaz Pro Ser Asp Asn 200 <210> 1132 <211> 201 <212> PRT <213> Corynebacterium glutanicun <400> 1132 Met Ser Asp Phe Tyr Ala Asp Arg I 1 5 Glu Vai Ala Pro Gly Met Leu Leu '1 Giu Asp Phe Giu Arg Ser Ile Val I Thr Thr Phe Gly Val Asn Ile Ser Asn Vai Leu Pro Giu Trp, Val Asp 1 70 Tyr Ile Gly Giy Pro Leu Ser Gin C Thr Lys Pro Gly Val Asp Ile Glu 1001 Ala Asn Arg Leu Vai His Vai Asp 1 115 120 ~eu Gly Val 95 ~ac aag ctc ~sn Lys Leu jaa-gat gtg liu Asp Val ~cg gag tgg lia Giu Trp :gg ttc gtc 'rp Phe Val 160 ~tc gat att Tai Asp Ilie 175 :tg tat tcc .eu Tyr Ser iattt aaa Thr Lys Pro gcc Ala gc t Ala gc t Ala 145 aca Thr tgg Trp acg Thr aac Asn gat Asp 130 ccg Pro cct Pro ggc Gly ttt Phe PCTJIBOO/00923 Gly Val 100 ctg gtg 451 Leu Val ctt gag 499 Leu Glu cag ctc 547 Gin Leu ttg ccg 595 Leu Pro 165 gtg atg 643 Val Met 180 tcg gac 691 Ser Asp Arg Ala Pro Val1 Ala Gly Lys Asp Asn Ser Ala Ala Leu Val1 Leu Val1 1539 WO 01/00843 WO 0100843PCTILBOOIOO923 Ala Asp 130 Asp Leu Glu Giy Arg Phe Phe Ala Gly 140 Tyr Ala Giu Trp Ala 145 Pro Gly Gln Leu Asn Glu Giu Ile Giu 150 Gln Gly Asp Trp, Phe Val 155 160 Thr Pro Ala Leu Pro 165 Ser Asp Ile Ile Pro Giy Arg Val Asp Ilie 175 Trp Gly Asp Thr Phe Pro 195 Met Arg Arg Gin Ala 185 Met Pro Leu Pro Leu Tyr Ser 190 Ser Asp Pro Ser Asp Asn 200 <210> <211> <212> 1133 1827

DNA

<213> Corynebacterium giutamicum <220> <221> <222> <223>

CDS

(101)..(1804) RXCO 0128 <400> 1133 ccattttccg tttggtcttg cctaaagaac cgcatggaaa ttatcgtgaa gcaccgatcc cgttgatcgc tccagagaca ccgtgggaag gggagcagca gtg agt aaa att tcg Val Ser Lys Ile Ser acg aaa ctg aag Thr Lys Leu Lys ctc acc gcg gtg ctg tct gtg acc act Leu Thr Ala Val Leu Ser Val Thr Thr ctg gtg Leu Val gct ggg tgt Ala-Gly Cys tca ttt tcc Ser Phe Ser acg ctt ccg cag Thr Leu Pro Gin acg gat ccg caa Thr Asp Pro Gin gtg ctg cgc Val Leu Arg ccg acc ccg Pro Thr Pro ggg tcc caa agc Gly Ser Gin Ser caa gag ata gca Gin Giu Ile Ala ggg Gly aat caa Asn Gin gat ccg gat ttg Asp Pro Asp Leu atc cgc ggc ttc Ile Arg Gly Phe agc gca ggt gcg Ser Aia Gly Ala 211 259 307 355 403 451 tat Tyr ccg act cag cag Pro Thr Gin Gin tat Tyr gaa gcg gcg aag Giu Aia Aia Lys tat ctg acg gaa Tyr Leu Thr Giu acg cgc agc acg Thr Arg Ser Thr aat ccg gct gcg Asn Pro Ala Ala act cgt att ttg Thr Arg Ile Leu gat cgc Asp Arg 100 att gat ctg Ile Asp Leu act ctg cca ggt tcg acg aat gcg gaa Thr Leu Pro Gly Ser Thr Asn Ala Giu 110 cga acg att Arg Thr Ilie 115 1540 WO 01/00843 WO 0100843PCTIBOOIOO923 gcg atc Ala Ile cag ccg Gin Pro 135 gat ggg Asp Gly 150 aga aac Arg Asn gat cct Asp Pro gag tcg Giu Ser cct tcg Pro Ser 215 gcg tcg Ala Ser 230 gga aat Gly Asn tgg acg Trp Thr gac ggc Asp Gly ctc gcc 1027 Leu Ala 295 ttt gcg 1075 Phe Ala 310 agt cca 1123 Ser Pro cgt Arg 120 gag Giu gag Glu ga t Asp tct Ser cag Gin 200 ccg Pro ttc Phe ttg Leu ttg Leu gcg Ala 280 gaa Giu t tg Leu cta Leu acg Thr gcg Ala cgt Arg cgg Arg 170 cag Gin atg Met att Ile 9g9 Gly gat Asp 250 cat His ttg Leu aac Asn gat Asp 9gc Gly 330 t tg Leu gag Giu ccg Pro ccg Pro 175 ga t Asp atg Met gtc Val1 gag Glu cgt Arg 255 ggc Giy cca Pro acc Thr agg Arg gga Gly 335 ctc Leu atc Ile gac Asp 160 cac His cgg Arg gcc Ala aa t Asn tat Tyr 240 ttc Phe ccc Pro acg Thr aac Asn gtc Val1 320 gat Asp agc Ser acg Thr 145 ggg Gly gat Asp cg t Arg ctt Leu cag Gin 225 cag Gin gcc Ala tac Tyr ctc Leu acg Thr 305 agt Ser atc Ile ggt Gly 130 a tg Met att Ile gtg Val1 tgg Trp c tg Leu 210 ctg Leu t tc Phe gcc Ala act Thr ac c Thr 290 gtg Val1 tcc Ser gat Asp tat Tyr gaa Glu gag Giu 165 ttt Phe aat Asn ggt Gly gat Asp ttg Leu 245 gtg Val gc t Ala gac Asp t tg Leu gtg Val1 325 gcg Ala 499 547 595 643 691 739 787 835 883 931 979 1541 WO 01/00843 WO 0100843PCTIBOOIOO923 att tcc 1171 Ile Ser gag gca 1219 Giu Ala tcc tcc Ser Ser, 345 gtg ctt Val Leu gcc Ala aat gtg gtg Asn Vai Val .360 ttg agg agt 1267 Leu Arg Ser 375 ggg ttg tgg 1315 Gly Leu Trp 390 tcg gca aca 1363 Ser Ala Thr act gtt ggc tcc Thr Val Gly Ser 365 acg atc act cgt.

Thr Ile Thr Arg 380 gca gcg gta cgc cac gaa aac aac Ala Ala Val Arg His Glu Asn Asn 350 355 atg gaa ggc gtg act tca gat gcg Met Glu Gly Val Thr Ser Asp Ala 370 ccc acc ttt gaa tac gcg tcg agt Pro Thr Phe Glu Tyr Ala Ser Ser 385 gaa Glu gct gtg gtg Ala Val Val 395 acc ggt gag Thr Gly Giu gat ggg gag acg cct Asp Gly Glu Thr Pro 400 ctc gtc cag acg gag Leu Val Gin Thr Giu 415 ccg atc tct gaa ttc Pro Ile Ser Giu Phe gtc cga gtc gca cga Val Arg Val Ala Arg 405 gcg gag att gtg ctg Ala Giu Ile Val Leu 420 caa ctg tca cga act Gin Leu Ser Arg Thr 435 gtg tac gtg ggc gtc Val Tyr Val Gly Val cca agg 1411 Pro Arg ggg gtc 1459 Gly Val gta acg 1507 Val Thr 455 gtg gcg 1555 Val Ala 470 gac ggc 1603 Asp Gly cgc gtc 1651 Arg Val ctc agc 1699 gat gtg Asp Val 425 cgg gcc Arg Ala 440 cgt cct Arg Pro ccg agc Pro Ser ggt Gly 430 gcc atg atc att Ala Met Ile Ile 445 ggt ccg ggc gag Gly Pro Gly Giu 460 ttg ggc gag gcg Leu Gly Glu Ala gaa-ggc aag Giu Gly Lys 450 cgg cgc gtg aca aat atc acg gag Arg Arg Val Thr Asn Ile Thr Giu 465 475 att ttg ctt gtg Ile Leu Leu Val 490 ggc acg Giv Thr gcg ctg tcg atc Ala Leu Ser Ile 480 tca att cca gag Ser Ile Pro Giu aac tgg cgc cca Asn Trp Arg Pro 485 acg ccg ctg tgg Thr Pro Leu Trp 495 500 gag cag Giu Gin 505 gcg ccg gac gga tcg gcg att Asp Gly Ser Ala Ile 510 gtg gtg gcg gtg gca tcg tcg atg ccg agc ggg aat Ser Ser Met Pro Ser Gly Asn 515 agt tcc gcg acg acg gtc tac Leu Ser Ala Pro Val Val Ala Val Ala Ser Ser Ala Thr Thr Vai Tyr 1542 WO 01/00843 gtc act ga 1747 Val Thr As 535 att tgg cg 1795 Ile Trp Ar 550 gtt gcg ta 1827 Val Ala Tv PCT/BOO/00923 tcg cat gcg atg ctt cag ctg ccg act gcc gat aat gat Ser His Ala Met Leu Gin Leu Pro Thr Ala Asp Asn Asp 540 545 gag gtg ccc ggt ttg ctg ggc acg Cgt gcg gcg ccg gtg Glu Val Pro Gly Leu Leu Gly Thr Arg Ala Ala Pro Val 555 560 565 tgatggagct gttcttcccg cgc <210> 1134 <211> 568 <212> PRT <213> Corynebacteriut giutamicum <400> 1134 Val Ser Lys Ile Ser Thr Lys Leu Lys 1 Val1 Pro Ala Phe Tyr Arg Ala Ser Thr 145 Giy Asp Arg Thr Val Pro Ala Thr Leu Arg 115 Giy Arg Leu Tyr Leu 195 Leu Leu Thr Gly Glu Asp 100 Thr Val1 Arg Leu Phe 180 Phe Gly Phe Gin Pro Arg Asp Ile Pro 135 Gly Asn Pro Ser Cys Ser 40 Asp Thr Ser Leu Arg 120 Giu Giu Asp Ser Gin 200 Ser 25 Gly Pro Gin Thr Asn 105 Gly Asn Trp, Leu Gly 185 Ser Ala 10 Thr Ser Asp Gin Trp, Thr Thr Ala Arg Arg 170 Gin Met Leu Leu Gin Leu Tyr 75 Asn Leu Gin Giu Ile 155 Asn Val Ser Thr Pro Ser Leu Giu Pro Pro Val1 Phe 140 Asp His Leu Thr Ala Gin Thr Ile Ala Ala Gly Gly 125 Glu Ala Tyr Val Val1 205 Val1 Asn Gin Arg Ala Ala Ser 110 Thr Ala Leu Thr Gly 190 Leu Leu Thr Giu Gly Lys Ser Thr Leu Giu Pro Pro 175 Asp Met Ser Asp Ile Phe Ala Thr Asn Leu Ile Asp 160 His Arg Ala 1543 WO 01/00843 PCTIiBOO/00923 Asn Gly Pro Ser Pro Ala Ile Ser Pro Gly Vai Val Asn Leu Gin 225 Gin Ala Tyr Leu Thr 305 Ser Ile Arg Vai Glu 385 Val1 Ala Gin Vai Thr 465 Ile Glu Met Val1 Ser Thr Gin Leu 275 Thr Ser Gly Ser Glu 355 Ser Ala Val Ile Ser 435 Val1 Ile Trp Pro Ser 515 215 220 Thr Gly Ala 260 Vai Asp Thr Asn Ala 340 Asn Asp Ser Ala Val1 420 Arg Gly Thr Arg Leu 500 Gly Asp Leu 245 Val1 Ala Asp Leu Val1 325 Ala Asn Ala Ser Arg 405 Leu Thr Val1 Giu Pro 485 Trp Asn Ala 230 Gly Trp Asp Leu Phe 310 Ser Ile Giu Leu' Gly 390 Ser Pro Gly Val Val 470 Asp Arg Leu Ser Asn Thr Gly Ala 295 Ala Pro Ser Ala Arg 375 Leu Al a Arg Val1 Thr 455 Al a Gly Val1 Se r Phe Leu Leu Ala 280 Glu Leu Leu Ser Val1 360 Se r Trp Thr Asp Arg 440 Arg Pro Ile Giu Ala 520 Val Gly Asp Asp 250 Ala His 265 Pro Leu Tyr Asn Gin- Asp Gin Gly 330 Ser Ala 345 Leu Thr Giu Thr Ala Val Thr Gly 410 Val Thr 425 Ala Ala Pro Gly Ser Leu Leu Leu 490 Gin Asp 505 Pro Val Asn Ala Asp Ser Giu 300 Ser Trp Val1 Gly Thr 380 Asp Leu Pro Ile Gly 460 Giu Gly Ser Ala Asp Arg Val1 Giu 285 Ala Leu Ser Val1 Ser 365 Arg Gly Val1 Ile Ile 445 Glu Ala Thr Ala Val 525 Gly Leu Ala 270 Phe Tyr Ser Gly Ala 350 Met Pro Giu Gin Ser 430 Giu Arg Ala Ser Ile 510 Ala Giu Tyr 240 Arg Phe 255 Gly Pro Pro Thr Thr Asn Arg Val 320 Gly Asp 335 Ala Val Giu Gly Thr Phe Thr Pro 400 Thr Glu 415 Glu Phe Gly Lys Arg Val Leu Ser 480 Ile Pro 495 Ser Ser Ser Ser Ala Thr Thr Val Tyr Val Thr Asp Ser His Ala Met Leu Gin Leu Pro 1544 WO 01/00843 WO 0100843PCTIiBOO/00923 Thr Ala Asp Asn Asp Ile Trp Arg Giu Val Pro 545 550 555 Arg Ala Ala Pro Val Val Ala Tyr 565 Gly Leu Leu Gly Thr 560 <210> 1135 <211> 555 <212> DNA <213> Corynebacterium <220> <221> .CDS <222> (101)..(532) <223> RXC01709 <400> 1135 gcaagtggaa ccacgatggg giutanicun aacggtaact gctgccgctg cggtattact cgtagtttca gaaattaggt gtcgatgcag caatacggaa ctttgccaat gtg ttt gaa caa Val Phe Giu Gin 1 115 ctc ggg ctc acc Leu Gly Leu Thr ctt gca caa aca Leu Ala Gin Thr gga gcg ggc gca Gly Ala Gly Ala gcg ggg Ala Gly ggc ttg ggt Gly Leu Gly ggc gtg gac Gly Val Asp ttc Phe atg gca atg gcg ttg ttg tct gca ggg Met Ala Met Ala Leu Leu Ser Ala Gly 30 atg cgc tcc Met Arg Ser atg ctt gca Met Leu Ala atg att ctt aat Met Ile Leu Asn acc ggg ggt gaa Thr Gly Gly Glu cag gca Gin Ala gat tta gtc atc Asp Leu Val Ile gga gaa gga cgc Gly Giu Gly Arg att Ile gat gca cag acc Asp Ala Gin Thr ctc Leu agc ggg aaa gct Ser Gly Lys Ala act gga atc gcc Thr Gly Ile Ala cgg gca cgt gcg Arg Ala Arg Ala gga att cca gta Gly Ile Pro Val ctg Leu gcg gtt tgt ggg Ala Val Cys Gly agc cta ttg ggt Ser Leu Leu Gly cca gca Pro Ala 100 atc tca aat Ile Ser Asn tct gac atc Ser Asp Ilie 120 cta ttt gaa gac Leu Phe Giu Asp atc Ile 110 tac agc ttt acc Tyr Ser Phe Thr gat ttc gaa Asp Phe Giu 115 ttg gaa ggt Leu Giu Gly aat gaa tgc att Asri Giu Cys Ile cga Arg 125 aac ccg ctc cca Asn Pro Leu Pro att Ile 130 atc ggt ttt aac atc gcc aaa cat cat ctg agt Ile Gly Phe Asn Ile Ala Lys His His Leu Ser 135 140 tagcgatatt tcagcaaacc 552 1545 WO 01/00843 WO 0100843PCTLIBOO/00923 gat 555 <210> 1136 <211> 144 <212> PRT <213> Corynebacterium glutamicun <400> 1136 Val Phe Giu Gin Ala Leu Gly Leu Thr Thr Leu Ala Gin Thr Ala Gly 1 5 10 Ala Gly Ala Ala Gly Gly Leu Gly Phe Met Ala Met Ala Leu Leu Ser 25 Ala Gly Met Arg Ser Gly Val Asp Met Ile Leu Asn Glu Thr Gly Gly 40 Giu Lys Met Leu Ala Gin Ala Asp Leu Val Ile Thr Gly Glu Giy Arg 55 Ile Asp Ala Gin Thr Leu Ser Gly Lys Ala Pro Thr Gly Ile Ala Lys 70 75 Arg Ala Arg Ala Lys Gly Ile Pro Val Leu Ala Val Cys Gly Gin Ser 90 Leu Leu Gly Pro Ala Ile Ser Asn Glu Leu Phe Giu Asp Ile Tyr Ser 100 105 110 Phe Thr Asp Phe Giu Ser Asp Ilie Asn Giu Cys Ile Arg Asn Pro Leu 115 120 125 Pro Ile Leu Giu Gly Ile Gly Phe Asn Ile Ala Lys His His Leu Ser 130 135 140 <210> 1137 <211> 898 <212> DNA <213> Corynebacteriun glutainicum <22 0> <221> CDS <222> (101)..(898) <223> RXC02207 <400> 1137 gaatcggtga ctttgccaac accaatcaca caagcccttg atgatgtctc cctgtgactt ggtccaatta cattcactgg taatctgaaa ccttgtgaat atg cgc cgt cga tcc 115 Met Arg Arg Arg Ser 1 cgt gtg tcc cgt ttg ctt ccc gcc aca gct ttg ctg gcc tca act gca 163 Arg Val Ser Arg Leu Leu Pro Ala Thr Ala Leu Leu Ala Ser Thr Ala 15 1546 WO 01/00843 ctt ctt tta Leu Leu Leu ggc aag gca Gly Lys Ala PCIBOOIOO923 gca tgt acg caa Ala Cys Thr Gin gta acg gac tcc Val Thr Asp Ser ccg gat atg Pro Asp Met ccg gat ggc Pro Asp Gly act ccc gct gtc tcc ccc gca gca agc Thr Pro Ala Val Ser Pro Ala Ala Ser caa gta att gag ttc ggC Gin Val Ile Glu Phe Gly aac Asn atc act gac atg Ile Thr Asp Met gtc act gat ggt Val Thr Asp Gly gac Asp atc ctc ggt gta Ile Leu Gly Val acc gaa gac gca Thr Giu Asp Ala gct att ggt aca Ala Ile Gly Thr tcc gac ttc gaa Ser Asp Phe Giu ggt agc cag gtg Giy Ser Gin Vai ctg gac gtc gat Leu Asp Val Asp aag caa Lys Gin 100 tgc ggc gac Cys Gly Asp gat ggc gtt Asp Gly Val 120 acc gca acc ggc Thr Ala Thr Gly act ttc gtg ctc Thr Phe Val Leu ccc tgc gcc Pro Cys Ala 115 gat gag ttg Asp Giu Leu tat ttg att gat Tyr Leu Ile Asp gcc Ala 125 aag gac ccg gat Lys Asp Pro Asp cgt gca Arg Ala 135 act gac aag cca Thr Asp Lys Pro acg gtg gca gcc Thr Val Ala Ala acc agc gat gat Thr Ser Asp Asp cag Gin 150 ctt ctg gtg ggc Leu Leu Val Gly ggt gaa gat gaa Gly Giu Asp Giu ctc acc atc tac cgc Leu Thr Ile Tyr Arg 165 gag ggc gaa gag Giu Gly Giu Giu gaa acc ttc acc Giu Thr Phe Thr gcg ggt ccc aat Ala Gly Pro Asn acc cag Thr Gin 180 ctc atc gcc Leu Ile Ala tgg aac gaa Trp, Asn Giu 200 cct gtc att gat Pro Val Ile Asp cgc Arg 190 cac gac gcc gtt His Asp Ala Val gtg cgc acc Val Arg Thr 195 aac gac cgt Asn Asp Arg aac acc acg att Asn Thr Thr Ile caa Gin 205 gat gtg gac tac Asp Val Asp Tyr gaa ggc Giu Gly 215 gcg acc ctt cgc Ala Thr Leu Arg gga ctc ggc gtt Gly Leu Gly Val caa atg gct ggt Gin Met Ala Gly 9gc Gly 230 gaa gac ggc ctg Giu Asp Gly Leu gtg gtc tct gat Val Val Ser Asp atg ggt ggc caa Met Gly Gly Gin att Ile 245 gcc atc tac aac Ala Ile Tyr Asn gat gat gtc atc Asp Asp Val Ile cga Arg 255 ctt caa aat gac Leu Gin Asn Asp cgc ccc Arg Pro 260 1547 WO 01/00843 cac cga cga gga acc His Arg Arg Gly Thr 265 PCTIBOOIOO923 898 <210> 1138 <211> 266 <212> PRT <213> Corynebacteriun glutamicun <400> 1138 Met Arg Arg Arg Ser Arg Val Ser Arg 1 Leu Asp Ser Giu Ala Asp Val1 Asp Leu 145 Leu Gly Ala Tyr Gly 225 Met Gin Ser Pro Pro Thr Gly Asp Pro 115 Asp Ser Ile Asn Val1 195 Asn Met Giy Asp Thr Asp Asp Asp Thr Lys 100 Cys Giu Asp Tyr Thr 180 Arg Asp Ala Gin Arg 260 5 Ala Met Gly Gly Val1 Gin Ala Leu Asp Arg 165 Gin Thr Arg Gly Ile 245 Pro Leu Gly Gin Asp 70 Ser Cys Asp Arg Gin 150 Glu Leu Trp Giu Gly 230 Ala His Leu Lys Val1 Ile Asp Gly Gly Ala 135 Leu Gly Ile Asn Gly 215 Giu Ile Arg Leu Ala 40 Ile Leu Phe Asp Val1 120 Thr Leu Giu Ala Giu 200 Ala Asp Tyr Arg Leu Cys Ala Gly Arg Gly Ala Ile Pro Asn 155 Glu Val Thr Arg Leu 235 Asp Pro Thr Val Asn Thr Ser Thr Asp Val 140 Gly Thr Ile Ile Val1 220 Val1 Asp Ala Gin Ser Ile Giu Gin Gly Ala 125 Thr Giu Phe Asp Gin 205 Gly Val1 Val Thr Gly Pro Thr Asp Val1 Gly 110 Lys Val1 Asp Thr Arg 190 Asp Leu Ser Ile Ala Val1 Ala Asp Al a Giu Thr Asp Ala Giu Val1 175 His Val1 Gly Asp Arg 255 1548 WO 01/00843 WO 0100843PCT/EBOO/00923 <210> <211> <212> <213> <220> <221> <222> <223> 1139 891

DNA

Corynebacterium glutamicun

CDS

(101)..(868) RXA0 0347 <400> 1139 tcggccagca atccgcttgg tgtcctggat cgcgccgaca tcttaaggtg ccagggcttt aaagtgccag gggttctgtg ggatccgtac actggttccc atg act ttg act Met Thr Leu Thr gag gaa atc gcc aag acc aaa aag ctt Giu Giu Ile Ala Lys Thr Lys Lys Leu gtt gtg tcc gat Val Val Ser Asp ttt gat Phe Asp gga acc atc Gly Thr Ile gga ttt agc aag Gly Phe Ser Lys gct tac aac gtt Ala Tyr Asn Val cct atc aac Pro Ilie Asn gca gac act Ala Asp Thr cag aaa tcc ctc aag gcg gta aaa Gin Lys Ser Leu Lys Ala Val Lys gac ctc tcc caa Asp Leu Ser Gin gat gtt Asp Val gtc att ttg tcg Val Ile Leu Ser cgt cac ctg gag Arg His Leu Giu ttg aag acg gtt Leu Lys Thr Val ctt Leu gat ctt ggt cag Asp Leu Gly Gin tac Tyr gac atc acc atg Asp Ile Thr Met ggt tca cac ggt Gly Ser His Gly gag gat tcc tcc Giu Asp Ser Ser ccg cgt acc ctc Pro Arg Thr Leu cct gaa gag gta Pro Glu Giu Val gct cgc Ala Arg 100 ctc gcc aag Leu Ala Lys ggc gca ttc Gly Ala Phe 120 at t Ile 105 gaa gca gat ctg Giu Ala Asp Leu aag atc gtc gac Lys Ile Val Asp ggc atc gaa Gly Ile Giu 115 cac ttc atc His Phe Ile gtg gag atc aag Val Giu Ile Lys cct Pro 125 ttc cac cgc gtg Phe His Arg Val c tg Leu 130 cgt gtt Arg Vai 135 tcc gac aag gac Ser Asp Lys Asp gtc caa gga atc Val Gin Gly Ile ctc Leu 145 gcc caa gca gca Ala Gin Ala Ala gta gac tct tcc Vai Asp Ser Ser ctg aag gtt act Leu Lys Val Thr ggc aag agc atc Giy Lys Ser Ile gaa tac tcc atc agc tcc acc acc aag ggc acc tgg ctg aag gaa tac Giu Tyr Ser Ile Ser Ser Thr Thr Lys Gly Thr Trp, Leu Lys Glu Tyr 1549 WO 01/00843 WO 0100843PCTIIBOO/00923 170 gtt gac cgc acc gag ccc act ggt gtg Val Asp Arg Thr Giu Pro Thr Giy Val 185 190 acc gat gag cac ggt ttc aaa gct tta Thr Asp Giu His Gly Phe Lys Ala Leu 200 205 acc gtc aag gtt ggc gaa gga gac act Thr Val Lys Val Gly Giu Gly Asp Thr 215 220 gat gtt gat aat gtg gga att ttc cta Asp Val Asp Asn Val Gly Ile Phe Leu 230 235 atg cag tat gcg gaa agc gtg cga ttg Met Gin Tyr Ala Glu Ser Val Arg Leu 250 a aa <210> 1140 <211> 256 <212> PRT <213> Corynebacteriun glutainicum <400> 1140 Met Thr Leu Thr Ile Giu Giu Ile Ala 175 att Ile gaa Giu gca Ala gag Glu ggg Gly 255 180 ctc ggc gat gac acc 691 Leu Gly Asp Asp Thr 195 gat gat cgt gcc cta 739 Asp Asp Arg Ala Leu 210 aaa acc cgc gtc gac 787 Lys Thr Arg Val Asp 225 ctc gcc tac cac cgc 835 Leu Ala Tyr His Arg 245 taagagagcc taaacgcacg 888 1 Val1 Asn Gin Gly Gly Giu Val Val 5 Asp Asn Thr Val Ser Arg Glu Ile Thr Lys Val1 Asp Asp Ala Ala Val1 135 Ala Leu Ile Gly Ser Ile 105 Val Asp Lys 10 Gly Lys Leu Gin Arg 90 Giu Giu Lys Ser Lys Ser Val1 Gly Asp Arg Asp Lys Lys 140 Leu Lys Lys Lys Arg Ile Thr Leu Pro 125 Val1 Lys Leu Asp Asp His Thr Leu Giu 110 Phe Gin Val1 Leu Ala Leu Leu Met Thr Lys His Gly Thr Leu Ala Gin Ala Ala His Val Asp Ser 145 150 1550 WO 01/00843 Gly Lys Ser ?zrp Leu Lys Leu Gly Asp 195 Asp Asp Arg 210 Lys Thr Arg 225 Leu Ala Tyr Ile Giu 180 Asp Ala Val His Ser 170 Giu Gly Giy Val Giu 250 PCTIBOO/00923 Gly Thr 175 Ile Phe Giu Asn Ala Ala Giu Lys 240 Gly Ile 255 <210> 1141 <211> 2556 <212> DNA <213> Corynebacterium glutamicun <22 0> <221> CDS <222> (2533) <223> RXN01239 <400> 1141 gcacttgctg cgtaaatctt tttcccacgc cgggaatgcg gtaccgcacg attttgccta acttttaagg gtgtttcatc tcc Ser ggg Gly aag Lys atg Met aat Asn ac a Thr gca Ala cgt Arg aag Lys cca Pro gaa Glu cac His acg Thr tca Ser cta Leu gat Asp gag Glu gag Glu ctt caa atg cga Leu Gin Met Arg gcc Ala t ac Tyr gat Asp ggt Gly atc Ile 1551 gga cct Gly Pro aaa gcc Lys Ala ctc tcc Leu Ser gtc att Val Ile ctt cga Leu Arg att gat Ile Asp tgaacactaa gatcgaggac atg gca cgt cca att Met Ala Arg Pro Ile 1 caa gca gat agc gcc Gin Ala Asp Ser Ala cag ctt ccc tat ctg Gin Leu Pro Tyr Leu cct att ttt acg gcc Pro Ile Phe Thr Ala gat ccc acc acc atc Aso Pro Thr Thr Ile WO 01/00843 WO 0100843PCT/iBOO/00923 tta ggt gtt Leu Gly Val aaa aac ggc Lys Asn Gly 120 gtt cca cat ttg aat cct tgg tgg tgg Val Pro His Leu Asn Pro Trp Trp Trp 110 100 gat gtt cta Asp Val Leu 115 att gac t99 Ile Asp Trp aaa gat tcc gct Lys ASP Ser Ala tt t Phe 125 gag ttc tat ttc Giu Phe Tyr Phe gat Asp 130 cac gaa gac aac ggt tct His Giu Asp Asn Gly Ser ggt Gly 140 ggc aag ctg ggc Gly Lys Leu Gly ccg att ctg ggt Pro Ile Leu Gly gc t Ala 150 gaa ggc gat gaa Giu Gly Asp Giu gac Asp 155 aag ctg gaa ttc Lys Leu Giu Phe gag ctt gat gga Glu Leu Asp Gly aaa gtg ctc aaa Lys Val Leu Lys ttt gac cac ctc Phe Asp His Leu cca atc gcg cct Pro Ile Ala Pro ggt acc Gly Thr 180 gaa gaa ggg Giu Glu Gly cag ttc tgg Gin Phe Trp 200 ccg caa gaa gtc Pro Gin Giu Val aag cgc cag cat Lys Arg Gin His tac cgc ctg Tyr Arg Leu 195 ttt tcc gtg Phe Ser Val cgc gat ggc gtg Arg Asp Gly Val atc Ile 205 aac ttc cgt cgc Asn Phe Arg Arg aat acg Asn Thr 215 ttg gct ggc atc Leu Ala Gly Ile agg Arg 220 caa gaa gat ccc Gin Giu Asp Pro gtg ttt gaa cat Val Phe Giu His act Thr 230 cat cgt ctg ctg His Arg Leu Leu cgc Arg 235 gaa ttg gtg gcg Giu Leu Val Ala gac ctc att gac Asp Leu Ile Asp ggc Gly 245 gtg cgc gtc gat Val Arg Vai Asp ccc gac ggg ctt Pro Asp Gly Leu tcc gat cct ttt gga tat ctg Ser Asp Pro Phe Giy Tyr Leu 255 260 cac aga ctc His Arg Leu aag atc ttg Lys Ile Leu 280 gac ctc att gga Asp Leu Ile Gly cc t Pro 270 gac cgc tgg ctg Asp Arg Trp Leu atc atc gaa Ile Ile Giu 275 gcc gtt gat Ala Val Asp agc gtt gat gaa Ser Vai Asp Giu cca Pro 285 ctc gat ccc cgc Leu Asp Pro Arg ggc acc act ggc tac gac gcc ctc cgt gaa ctc gac ggc gtg ttt atc 1027 Giy Thr 295 tcc cga 1075 Ser Arg 310 Thr Gly Tyr Asp Ala 300 Leu Arg Giu Leu Gly Val Phe Ile gaa tct gag gac aaa ttc tcc atg ctg gcg ctg acc cac agt Glu Ser Giu Asp 315 Lys Phe Ser Met Ala Leu Thr His Ser 325 1552 WO 01/00843 WO 0100843PCTILBOOIOO923 gga tcc 1123 Gly Ser aaa cga 1.171 Lys Arg gcc cgc 1219 Ala Arg acc gaa 1267 Thr Glu 375 ccc gtc 1315 Pro Val 390 gtc atc 1363 Val Ile gac ctc 1411 Asp Leu ttc gct 1459 Phe Ala acc tgg Thr Trp gtc gtc Val Val 345 gcc atg Ala Met gat Asp 330 gcc Ala gaa cgc gcc ctc aaa Giu Arg Ala Leu Lys 335 caa caa gaa ctc gca Gin Gin Glu Leu Ala 350 cgc cgc gat aac ttc Arg Arg Asp Asn Phe tcc acg gag gaa agc ctc Ser Thr Giu Glu Ser Leu 340 gcc gaa atc tta agg ctc Ala Glu Ile Leu Arg Leu 355 acc gca ggc acc aac gtc Thr Ala Giy Thr Asn Val 370 gaa tta gtc gcc gcc atg Giu Leu Val Ala Ala Met 360 gac Asp aaa ctt agc gaa Lys Leu Ser Giu 380 365 acc Thr atc atc Ile Ile 385 tac cgc gcc gac Tyr Arg Ala Asp 395 gcg gag atg tcc Aia Giu Met Ser 410 tac atc tcc ctc Tyr Ile Ser Leu aaa cgc ttc ccc Lys Arg Phe Pro tca Ser 400 cgc Arg acc acc gcc acc Thr Thr Ala Thr 405 atc gcg Ile Ala 425 caa gtc Gln Val 440 gcc gcc cta ctt ggc Ala Ala Leu Leu Gly 430 tgc ggc gcc gtc atg Cys Gly Ala Val Met 415 aat Asn gct Ala tcc cgg cgt gac gca ctc Ser Arg Arg Asp Ala Leu 420 ggc gag gcc aaa atc cgc Gly Glu Ala Lys Ile Arg 435 aaa ggt gtg gaa gac acc Lys Gly Val Glu Asp Thr acc ttc tac cgc gca 1507 Thr Phe Tyr Arg Ala 455 gcg ccg ggg aga ttc 1555 Ala Pro Gly Arg Phe 470 tct agg Ser Arg 460 gtt Val1 450 gca ttg caa gaa gtc ggt ggc Ala Leu Gln Giu Val Gly Gly 465 ggc Gly gaa gaa 1603 Giu Glu cat gac 1651 His Asp cgc agc ctg Arg Ser Leu 490 acc aaa cgt Thr Lys Arg 505 gtc tcc gct gca gaa Val Ser Ala Ala Giu 480 tgg cca cgc acc atg Trp Pro Arg Thr Met 495 gaa gat acc cgc gcc Glu Asp Thr Arg Ala 510 t tc Phe cac ttg ctg cag His Leu Leu Gin 485 acc acc ttg tcc acg Thr Thr Leu Ser Thr 500 cgc atc atc tcc ctg Arg Ile Ile Ser Leu 515 ggc Gly 1553 WO 01/00843 WO 0100843PCTJIBOOIOO923 tct gaa 1699 Ser Glu gtg ctc 1747 Val1 Leu 535 ctg ctg 1795 Leu Leu 550 gat cga 1843 Asp Arg aaa acc 1891 Lys Thr gat tgg 1939 Asp Trp gaa ttt 1987 Giu Phe 615 agg aaa 2035 Arg Lys 630 gga act 2083 Gly Thr ttt gtt 2131 Phe Val tgg gct 2179 Trp Ala gac atc 2227 Asp Ile 695 gtC Val 520 ccc gat atg tac tcc Pro Asp Met Tyr Ser 525 gag ctg gtc aat cgt gtt ttc gcg Glu Leu Val Asn Arg Val Phe Ala 530 acg ggc agt ttc ctc cta caa aac Thr Giy Ser Phe Leu Leu Gin Asn 545 ccc gcg cca gac Pro Ala Pro Asp ggc gta tgg ccc Gly Vai*Trp Pro 555 ggc gca Gly Ala gcc gac ggc gtg atc Ala Asp Gly Val Ile 560 acc gat gcg ctg cgc Thr Asp Ala Leu Arg 565 cgc gaa gca tcc aca Arg Piu Ala Ser Thr 580 ttc agg Phe Arg acg tgg Thr Trp 585 gaa tac gcc cta aaa Giu Tyr Ala Leu Lys 570 gtg gac ccc aac gag Val Asp Pro Asn Giu 590 gcg ctt ttc gac gga Ala Leu Phe Asp Gly gct Ala 575 atc Ile gaa Glu tcc ttc gag gct gcg gtc tgc Ser Phe Giu Ala Ala Val Cys 595 ccc tcc acc tca cta atc acc Pro Ser Thr Ser Leu Ile Thr 610 tct gtg caa atc tcc tta ggc Ser Val Gin Ile Ser Leu Gly 605 tcc cac atc aac cgt Ser His Ile Asn Arg 620 ggC Gly 625 ctg ctg caa Leu Leu Gin gag ttt tta Giu Phe Leu 650 gat tac acc Asp Tyr Thr 665 atg gtg ggc gct gga atc Met Val Gly Ala Gly Ile 635 640 gaa. gac tcc ctg gta gat Glu Asp Ser Leu Val Asp 655 ccc Pro ccc Pro gac act tac caa Asp Thr Tyr Gin 645 gat aac cga cgc Asp Asn Arg Arg 660 gcc Ala aga Arg acg cag gtt aat Thr Gin Val Asn aaa atg gcc gtg Lys Met Ala Val 700 gaa caa gtc ctg gag cgc ctg caa acc Giu Gin Val Leu Giu Arg Leu Gin Thr 670 675 tcg gta gaa gac ttg gtg gat aac gcc Ser Val Giu Asp Leu Vai Asp Asn Ala 685 690 gtC cat aaa tcc ctc gag ttg cgt gct Val His Lys Ser Leu Giu Leu Arg Ala 705 1554 WO 01/00843 WO 0100843PCTJIBOO/00923 gaa ttt cgt gca agc ttt gtt ggt gga gat cat cag gca gta ttt ggc 2275 Glu Phe Arg Ala Ser Phe Val Gly Gly Asp His Gin Ala Val Phe Gly 710 715 720 725 gaa ggt cgc gca gaa tcc cac atc atg ggc atc gcc cgc ggt aca gac 2323 Glu Gly Arg Ala Giu Ser His Ile Met Gly Ile Ala Arg Gly Thr Asp 730 735 740 cga aac cac ctc aac atc att gct ctt gct acc cgt cga cca ctg atc 2371 Arg Asn His Leu Asn Ile Ile Ala Leu Ala Thr Arg Arg Pro Leu Ile 745 750 755 ttg gaa gac cgt ggc gga tgg tat gac acc acc gtc acg ctt cct ggt 2419 Leu Glu Asp Arg Gly Gly Trp, Tyr Asp Thr Thr Val Thr Leu Pro Gly 760 765 770 gga caa tgg gaa gac agg ctc acc ggg caa cgc ttc agt ggt gtt gtc 2467 Gly Gin Trp Giu Asp Arg Leu Thr Giy Gin Arg Phe Ser Gly Val Val 775 780 785 ccagcc acc gat ttg ttc tca cat cta ccc gta tct. ttg ttg gtt tta 2515 Pro Ala Thr Asp Leu Phe Ser His Leu Pro Val Ser Leu Leu Val Leu 790 795 800 805 gta ccc gat agt gag ttt tgatccctgc acaggaaagt tag 2556 Val Pro Asp Ser Giu Phe 810 <210> 1142 <211> 811 <212> PRT <213> Corynebacterium giutamicum <400> 1142 Met Ala Arg Pro Ile Ser Ala Thr Tyr Arg Leu Gin Met Arg Giy Pro 1 5 10 Gin Ala Asp Ser Ala Giy Arg Ser Phe Giy Phe Aia Gin Ala Lys Ala 25 Gin Leu Pro Tyr Leu Lys Lys Leu Gly Ile Ser His Leu Tyr Leu Ser 40 Pro Ile Phe Thr Ala Met Pro Asp Ser Asn His Gly Tyr Asp Val Ile 55 Asp Pro Thr Thr Ile Asn Giu Glu Leu Gly Gly Met Glu Giy Leu Arg 70 75 Asp Leu Ala Ala Ala Thr His Glu Leu Gly Met Gly Ile Ile Ile Asp 90 Ile Val Pro Asn His Leu Gly Val Ala Val Pro His Leu Asn Pro Trp 1555 WO 01/00843 WO 0100843PCTIBOO/00923 100 105 110 Trp Trp Asp Val Leu LYS Asn Gly Lys Asp Ser Ala Phe Giu Phe Tyr 115 120 125 Phe Asp Ile Asp Trp, His Glu Asp Asn Gly Ser Gly Gly Lys Leu Gly 130 135 140 Met Pro Ilie Leu Gly Ala Giu Gly Asp Giu Asp Lys Leu Giu Phe Ala 145 150 155 160 Giu Leu Asp Giy Glu Lys Val Leu Lys Tyr Phe Asp His Leu Phe Pro 165 170 175 Ilie Ala Pro Gly Thr Giu Giu Giy Thr Pro Gin Giu Val Tyr Lys Arg 180 185 190 Gin His Tyr Arg Leu Gin Phe Trp Arg Asp Gly Val Ile Asn Phe Arg 195 200 205 Arg Phe Phe Ser Val Asn Thr Leu Ala Gly Ile Arg Gin Giu Asp Pro 210 215 220 *Leu Val Phe Giu His Thr His Arg Leu Leu Arg Glu Leu Val Ala Giu 225 230 235 240 Asp Leu Ile Asp Gly Val Arg Val Asp His Pro Asp Giy Leu Ser Asp 245 250 255 Pro Phe Gly Tyr Leu His Arg Leu Arg Asp Leu Ile Gly Pro Asp Arg 260 265 270 Trp Leu Ilie Ilie Glu Lys Ile Leu Ser Vai Asp Giu Pro Leu Asp Pro 275 280 285 Arg Leu Ala Val Asp Gly Thr Thr Gly Tyr Asp Ala Leu Arg Glu Leu 290 295 300 Asp Gly Val Phe Ile Ser Arg Giu Ser Giu Asp Lys Phe Ser Met Leu 305 310 315 320 Ala Leu*Thr His Ser Gly Ser Thr Trp Asp Giu Arg Ala Leu Lys Ser 325 330 335 Thr Giu Glu Ser Leu Lys Arg Val Val Ala Gin Gin Giu Leu Ala Ala 340 345 350 Glu Ilie Leu Arg Leu Ala Arg Ala Met Arg Arg Asp Asn Phe Ser Thr 355 360 365 Ala Gly Thr Asn Val Thr Glu Asp Lys Leu Ser Glu Thr Ile Ile Glu 370 375 380 Leu Val Ala Ala Met Pro Val Tyr Arg Ala Asp Tyr Ile Ser Leu Ser 385 390 395 400 Arg Thr Thr Ala Thr Val Ile Ala Glu Met Ser Lys Arg Phe Pro Ser 405 410 415 Arg Arg Asp Ala Leu Asp Leu Ile Ala Ala Ala Leu Leu Gly Asn Gly 420 425 430 1556 WO 01/00843 WO 0100843PCTIIBOO/00923 Giu Gly Gin 465 Phe Thr Arg Asn Phe 545 Thr Arg Glu Thr Gin 625 Pro Pro Glu Leu Leu 705 Gin Ala Ala Val1 450 Glu His Thr Ile Arg 530 Leu Asp Glu Al a Ser 610 Ile Asp Asp Arg Val1 690 Giu Ala Arg Lys 435 Glu Val1 Leu Leu Ile 515 Val Leu Ala Al a Al a 595 Leu Ser Thr Asn Leu 675 Asp Leu Val Gly Ile Asp Gly Leu Ser 500 Ser Phe Gin Leu Ser 580 Val Ile Leu Tyr Arg 660 Gin Asn Arg Phe Thr 740 Cys Ala Phe Leu 490 Arg Asp Pro Trp Glu 570 Val Ala His Gin Leu 650 Thr Gin Met Ser Glu 730 Asn Gly Ser Gly 475 Leu Gly Met Asp Pro 555 Tyr Asp Leu Ile Met 635 Giu Ala Val Ala Phe 715 Ser Ile Met Val1 Ala Arg Thr 510 Glu Thr Gly Lys Glu 590 Gly Gly Ala Leu Gin 670 Val His Gly Met Leu 750 1557 WO 01/00843 PCT/IBOO/00923 Arg Arg Pro Leu Ile Leu Giu Asp Arg Gly Gly Trp, Tyr Asp Thr Thr 755 760 765 Val Thr Leu Pro Gly Gly Gin Trp Giu Asp Arg Leu Thr Gly Gin Arg 770 775 780 Phe Ser Gly Val Val Pro Ala Thr Asp Leu Phe Ser His Leu Pro Vai 785 790 795 800 Ser Leu Leu Val Leu Val Pro Asp Ser Glu Phe 805 810 <210> 1143 <211> 2556 <212> DNA <213> Cory-nebacteriut glutamicum <220> <221> CDS <222> (2533) <223> FRXA0i239 <400> 1143 gcacttgctg cgtaaatctt tttcccacgc cgggaatgcg tgaacactaa gatcgaggac gtaccgcacg attttgccta acttttaagg gtgtttcatc atg gca cgt cca att Met Ala Arg Pro Ile tcc gca Ser Ala ggg cgt Gly Arg aag aag Lys Lys atg cca Met Pro aat gaa Asn Giu aca cac Thr His tta ggt Leu Gly aaa aac Lys Asn acg Thr tca Ser cta Leu ga t Asp gag Giu gag Giu gtt Val1 Giy 120 tac Tyr t tt Phe ggC Gly tcc Ser ctc Leu t tg Leu gcc Ala 105 aaa Lys agg Arg ggt G ly atc Ile aat Asn gg t Gly ggC Gly gtt Val1 gat Asp ctt caa Leu Gin ttt gcg Phe Ala agc cac Ser His cat His 99C Gly 75 atg Met cca Pro tcc Ser 99C Gly 60 atg Met ggc Gly cat His gct Ala atg Met cag Gin ctg Leu 45 tac Tyr gag Giu atc Ile t tg Leu tt t Phe 125 cgaI ArgI gcc Ala 30 tac TyrI gat Asp' ggt GlyI atc Ile aat Asn 110 gag Glu 1558 1 caa Gin cag Gin cc t Pro gat Asp ga t Asp att Ile tgg Trp ttc Phe gca Ala c tt Leu att Ile ccc Pro ctt Leu gtt Val1 tgg Trp gat Asp 130 ga t Asp ccc Pro ttt Phe acc Thr gcc Ala ccc Pro gat Asp 115 att Ile agc Ser tat Tyr acg Thr acc Thr gca Ala aac Asn 100 gtt Val1 gac Asp gcc Ala ctg Leu gcc Ala atc Ile gct Ala cat His cta Leu tgg Trp, WO 01/00843 WO 0100843PCTIiBOO/00923 c *ac gaa gac aac His Glu Asp Asn 135 gct gaa Ala Giu 150 aaa gtg Lys Val gaa gaa Giu Glu cag ttc Gin Phe aat acg Asn Thr 215 act cat Thr His 230 gtg cgc Val Arg cac aga His Arg aag atc Lys Ile ggc acc 1027 Gly Thr 295 tcc cga 1075 Ser Arg 310 gga tcc 1123 Gly Ser aaa cga 1171 ggc Gly c tc Leu ggg Giy tgg Trp 200 ttg Leu cgt Arg gtc Vali ctc Leu ttg Leu 280 act Thr gaa Giu acc Thr gtc gat Asp aaa Lys aca Thr 185 cgc Arg gct Ala c tg Leu gat Asp cgc Arg 265 agc Ser ggc Gly tct Ser tgg Trp gtc ggt tct Gly Ser gaa gac Giu Asp 155 tat ttt Tyr Phe 170 ccg caa Pro Gin gat ggc Asp Gly ggc atc Giy Ile ctg cgc Leu Arg 235 cac ccc His Pro 250 gac ctc Asp Leu gtt gat Vai Asp tac gac Tyr Asp gag gac Giu Asp 315 gat gaa Asp Giu 330 gcc caa ggt ggc Gly Gly 140 aag ctg Lys Leu gac cac Asp His gaa gtc Giu Val gtg atc Val Ile 205 agg caa Arg Gin 220 gaa ttg Giu Leu gac ggg Asp Gly att gga Ile Gly gaa cca Giu Pro 285 gcc ctc Ala Leu 300 aaa ttc Lys Phe cgc gcc Arg Ala caa gaa aag ctg ggc atg ccg att Lys Leu Gly Met Pro Ile 145 gaa Glu c tc Leu tac Tyr 190 aac Asn gaa Giu gtg Vai c tt Leu cct Pro 270 ctc Leu cgt Arg tcc Ser ctc Leu ctc ttc Phe ttc Phe 175 aag Lys ttc Phe gat Asp gcg Aia tcc Ser 255 gac Asp gat Asp gaa Giu atg Met aaa Lys 335 gca gcg Ala 160 cca Pro cgc Arg cgt Arg ccc Pro gaa Giu 240 gat Asp cgc Arg ccc Pro ctc Leu ctg Leu 320 tcc Ser gcc gag Giu atc Ile cag Gin cgc Arg tta Leu 225 gac Asp cct Pro tgg Trp cgc Arg gac Asp 305 gcg Ala acg Thr gaa ctt Leu gcg Aia cat His ttc Phe 210 gtg Val1 ctc Leu ttt Phe c tg Leu ctg Leu 290 ggc Gly ctg Leu gag Giu atc gat Asp cc t Pro tac Tyr 195 ttt Phe ttt Phe att Ile gga Gly a tc Ile 275 gcc.

Ala gtg Val1 acc Thr gaa Giu tta ctg Leu gga Gly gg t Giy 180 cgc Arg tcc Ser gaa Glu gac Asp tat Tyr 260 atc Ile gtt Val ttt Phe cac His agc Ser 340 agg ggt Gly gag Giu 165 ac c Thr ctg Leu gtg Val1 cat His ggc Gly 245 ctg Leu gaa Giu gat Asp atc Ile agt Ser 325 ctc Leu ctc 547 595 643 691 739 787 835 883 931 979 Lys Arg Val Val Ala Gin Gin Giu Leu Ala Ala Giu Ile Leu Arg Leu 345 350 355 1559 WO 01/00843 WO 0100843PCT/IBOO/00923 gcC cgc 1219 Ala Arg acc gaa 1267 Thr Giu 375 ccc gtc 1315 Pro Val 390 gtc atc 1363 Val Ile gac ctc 1411 Asp Leu ttc gct 1459 Phe Ala acc ttc 1507 Thr Phe 455 gcg ccg 1555 Ala Pro 470 gaa gaa 1603 Glu Giu cat gac 1651 His Asp tct gaa 1699 Ser Glu gtg ctc 1747 Val Leu 535 atg cgc cgc gat Met Arg Arg Asp aaa ctt agc gaa Lys Leu Ser Glu aac Asn 365 ttc tcc acc gca ggc acc aac gtc Phe Ser Thr Ala Gly Thr Asn Val 370 tac cgc gcc gac Tyr Arg Ala Asp 395 gcg gag atg tcc Ala Giu Met Ser 410 atc gcg gcc gcc Ile Ala Ala Ala 380 t ac Tyr aaa Lys acc atc atc gaa tta Thr Ile Ile Glu Leu 385 atc tcc ctc tca cgc Ile Ser Leu Ser Arg 400 gtc gcc gcc atg Val Ala Ala Met acc acc gcc acc Thr Thr Ala Thr 405 cgc ttc ccc Arg Phe Pro 415 Cta ctt ggc Leu Leu Gly 430 aat Asn tcc cgg cgt gac gca ctc Ser Arg Arg Asp Ala Leu 420 ggc gag gcc aaa atc cgc Gly Giu Ala Lys Ile Arg 435 aaa ggt gtg gaa gac acc Lys Gly Val Glu Asp Thr caa Gin 425 440 t ac Tyr gtc tgc ggc gcc gtc Val Cys Gly Ala Val 445 cgc gca tct agg ctc Arg Ala Ser Arg Leu atg gct Met Ala 450 gtt gca ttg caa gaa gtc ggt ggc Val Ala Leu Gin Glu Val Gly Gly ggg aga ttc ggc Gly Arg Phe Gly 475 cgc agc ctg ctg Arg Ser Leu Leu 490 acc aaa cgt ggc Thr Lys Arg Gly tcc gct gca Ser Ala Ala gaa ttc cac ttg ctg cag Giu Phe His Leu Leu Gin 480 485 atg acc acc ttg tcc acg Met Thr Thr Leu Ser Thr 500 tgg cca cgc acc Trp Pro Arg Thr 495 gaa gat acc cgc Glu Asp Thr Arg gcc cgc atc atc tcc ctg Ala Arg Ile Ile Ser Leu 515 505 510 gtc Val1 520 ccc Pro ccc gat atg tac tcc Pro Asp Met Tyr Ser 525 gcg cca gac ggc gca Ala Pro Asp Gly Ala 540 gag ctg gtc aat cgt gtt ttc gcg Glu Leu Val Asn Arg Val Phe Ala 530 acg ggc agt ttc ctc cta caa aac Thr Gly Ser Phe Leu Leu Gin Asn 545 1560 WO 01/00843 WO 0100843PCT/EBOO/00923 ctg ctg 1795 Leu Leu 550 gat cga.

1843 Asp Arg aaa acc 1891 Lys Thr gat tgg 1939 Asp Trp, gaa ttt 1987 Glu Phe 615 agg aaa 2035 Arg Lys 630 gga act 2083 Gly Thr ttt gtt 2131 Phe Val tgg gat 2179 Trp Asp gac atc 2227 Asp Ile 695 gaa ttt 2275 Giu Phe 710 gaa ggt 2323 Glu Gly ggc gta tgg ccc Gly Val Trp, Pro 555 ttc agg gaa tac Phe Arg Giu Tyr 570 gcc gac ggc gtg atc Ala Asp Gly Val Ile 560 gcc cta aaa gct atc Ala Leu Lys Ala Ile 575 acc gat gcg ctg cgc Thr Asp Ala Leu Arg 565 cgc gaa gca tcc aca Arg Glu Ala Ser Thr 580 gag gct gcg gtc tgc Giu Ala Ala Val Cys 595 acc tca cta atc acc Thr Ser Leu Ile Thr acg tgg Thr Trp 585 gtg gaa Val Giu gtg gac ccc aac gag Val Asp Pro Asn Giu 590 gcg ctt ttc gac gga Ala Leu Phe Asp Gly 605 cac atc aac cgt ggc His Ile Asn Arg Gly tcc ttc Ser Phe ccc tcc Pro Ser tcc Ser 610 tct gtg caa atc tcc tta ggc Ser Val Gin Ile Ser Leu Gly 620 625 ctg ctg caa atg Leu Leu Gin Met 635 gag ttt tta gaa Giu Phe Leu Giu 650 gtg ggc gct gga atc Val Gly Ala Gly Ile 640 gac tcc ctg gta gat Asp Ser Leu Val Asp 655 aga gaa caa gtc ctg Arg Giu Gin Val Leu ccc Pro gac act tac caa Asp Thr Tyr Gin 645 ccc gat aac cga cgc Pro Asp Asn Arg Arg 660 gag cgc ctg caa acc Giu Arg Leu Gin Thr gat tac Asp Tyr 665 tgg acg Trp Thr 680 gcc aaa Ala Lys acc gcc Thr Ala 670 cag gtt aat tcg gta Gin Val Asn Ser Val 685 675 gaa gac Giu Asp atg gcc gtg Met Ala Val cgt gca agc Arg Ala Ser cgc gca gaa Arg Ala Giu 730 700 ttt gtt Phe Val 715 tcc cac Ser His gtc cat aaa tcc Val His Lys Ser ggt gga gat cat Gly Gly Asp His 720 atc atg ggc atc Ile Met Gly Ile 735 ttg gtg gat aac gcc Leu Val Asp Asn Ala 690 ctc gag ttg cgt gct Leu Giu Leu Arg Ala 705 cag gca gta ttt ggc Gin Ala Vai Phe Gly 725 gcc cgc ggt aca gac Ala Arg Giy Thr Asp 740 1561 WO 01/00843 cga aac ca 2371 Arg Asn Hi ttg gaa ga 2419 Leu Giu As 76 gga caa tg 2467 Gly Gin Tr 775 cca gcc ac 2515 Pro Ala T1h 790 gta ccc ga 2556 Val Pro As att gct ctt gct acc cgt cga Ile Ala Leu Ala Thr Arg Arg 750 tgg tat gac acc acc gtc acg Trp Tyr Asp Thr Thr Val Thr 765 770 ctc acc ggg caa cgc ttc agt Leu Thr Gly Gin Arg Phe Ser 780 785 tca cat cta ccc gta tct ttg Ser His Leu Pro Vai Ser Leu 800 tgatccctgc acaggaaagt tag PCTIiBOO/00923 ctg atc Leu Ile cct ggt Pro Gly gtt gtc Val Val gtt tta Val Leu 805 <210> 1144 <211> 811 <212> PRT <213> Corynebacteriuni glutamicun <400> 1144 Met Ala Arg Pro Ile Ser Aia Thr Tyr 1 Gin Gin Pro Asp Asp Ile Trp Phe Met 145 Ser Tyr Thr Thr Ala Asn 100 Val Asp Leu Arg Lys Pro Giu His Gly Asn Glu 135 Glu Arg Gly Ile Asn Giy Giy Vai Asp Gly Giu Leu Phe Ser His Gly Met Pro Ser Ser Asp 155 Gin Aia His Giy Met Giy His Ala Gly 140 Lys Met Gin Leu Tyr Giu Ile Leu Phe 125 Gly Leu Arg Ala Tyr Asp Gly Ile Asn 110 Giu Lys Glu 1562 WO 01/00843 WO 0100843PCTIBOO/00923 Giu Ile Gin Arg Leu 225 Asp Pro Trp Arg Asp 305 Aia Thr Giu Aia Leu 385 Arg Arg Glu Gly Gin 465 Asp Pro Tyr 195 Phe Phe Ile Giy Ile 275 Ala Val1 Thr Glu Leu 355 Thr Ala Thr Asp Lys 435 Giu Val Gly Gly 180 Arg Ser Glu Asp Tyr 260 Ile Val1 Phe His Ser 340 Arg Asn Aia Al a Al a 420 Ile Asp Gly Glu 165 Thr Leu Val His Giy 245 Leu Giu Asp Ile Ser 325 Leu Leu Val Met Thr 405 Leu Arg Thr Gly Lys Glu Gin Asn Thr 230 Val1 His Lys Gly Ser 310 Giy Lys Ala Thr Pro 390 Val Asp Phe Thr Ala 470 Val Glu Phe Thr 215 His Arg Arg Ile Thr 295 Arg Ser Arg Arg Glu 375 Val1 Ile Leu Ala Phe 455 Pro Leu Gly Trp 200 Leu Arg Val Leu Leu 280 Thr Giu Thr Val Ala 360 Asp Tyr Ala Ile Gin 440 Tyr Gly Lys Thr 185 Arg Ala Leu Asp Arg 265 Ser Gly Ser Trp Val1 345 Met Lys Arg Glu Ala 425 Val1 Arg Arg Tyr Phe 170 Pro Gin Asp Gly Gly Ile Leu Arg 235 His Pro 250 Asp Leu Val Asp Tyr Asp Giu Asp 315 Asp Giu 330 Ala Gin Arg Arg Leu Ser Ala Asp 395 Met Ser 410 Ala Ala Cys Gly Ala Ser Phe Gly 475 Asp Giu Val Arg 220 Glu Asp Ile Glu Ala 300 Lys Arg Gin Asp Glu 380 Tyr Lys Leu Ala Arg 460 Val1 His Val Ile 205 Gin Leu Gly Gly Pro 285 Leu Phe Ala Giu Asn 365 Thr Ile Arg Leu Val1 445 Leu Ser Leu Tyr 190 Asn Glu Val1 Leu Pro 270 Leu Arg Ser Leu Leu 350 Phe Ile Ser Phe Gly 430 Met Val Al a Phe Pro 175 Lys Arg Phe Arg Asp Pro Ala Giu 240 Ser Asp 255 Asp Arg Asp Pro Glu Leu Met Leu 320 Lys Ser 335 Ala Ala Ser Thr Ile Glu Leu Ser 400 Pro Ser 415 Asn Gly Ala Lys Ala Leu Ala Glu 480 1563 WO 01/00843 Phe His Leu Thr Thr Leu Arg Ile Ile 515 Asn Arg Vai 530 Phe Leu Leu 545 Thr Asp Ala Arg Giu Ala Glu Ala Ala 595 Thr Ser Leu 610 Gin Ile Ser 625 Pro Asp Thr Pro Asp Asn Glu Arg Leu 675 Leu Val Asp 690 Leu Giu Leu 705 Gin Ala Val Ala Arg Gly Arg Arg Pro 755 Val Thr Leu 770 Phe Ser Gly 785 Leu Gin 485 Ser Thr 500 Ser Leu Phe Ala Gin Asn Leu Arg 565 Ser Thr 580 Val Cys Ile Thr Leu Gly Tyr Gin 645 Arg Arg 660 Gin Thr Asn Ala Arg Aia Phe Gly 725 Thr Asp 740 Leu Ile Pro Gly Val Val Giu His Ser Vai Leu 550 Asp Lys Asp Giu Arg 630 Giy Phe Trp Asp Giu 710 Glu Arg Leu Gly Pro 790 Glu Arg Asp Thr Giu Val 520 Leu Pro 535 Leu Gly Arg Phe Thr Thr Trp, Val 600 Phe Val1 615 Lys Leu Thr Glu Val Asp Asp Trp 680 Ile Ala 695 Phe Arg Gly Arg.

Asn His Glu Asp 760 Gin Trp 775 Ala Thr Ser Leu 490 Lys Arg 505 Pro Asp Ala Pro Vai Trp Arg Giu 570 Trp Val 585 Giu Ala Ser His Leu Gin Phe Leu 650 TIyr Thr 665 Thr Gin Lys Met Ala Ser Ala Giu 730 Leu Asn 745 Arg Giy Giu Asp Asp Leu Leu Gly Met Asp Pro 555 Tyr Asp Leu Ile Met 635 Giu Ala Val1 Al a Phe 715 Ser Ile Gly Arg Phe 795 Trp Giu Tyr Giy 540 Ala Al a Pro Phe Asn 620 Val Asp Arg Asn Val1 700 Val1 His Ile Trp Leu 780 Ser Pro Asp Ser 525 Al a Asp Leu Asn Asp 605 Arg Gly Ser Giu Ser 685 Val1 Giy Ile Al a Tyr 765 Thr His Arg Thr 510 Giu Thr Gly Lys Giu 590 Gly Gly Al a Leu Gin 670 Val1 His Giy Met Leu 750 Asp Gly Leu PCTIIBOO/00923 Thr Met 495 Arg Ala Leu Val Gly Ser Vai Ile 560 Ala Ile 575 Ser Phe Pro Ser Ser Val Gly Ile 640 Val Asp 655 Val Leu Glu Asp Lys Ser Asp His 720 Gly Ile 735 Ala Thr Thr Thr Gin Arg Pro Val 800 Ser Leu Leu Val Leu Val Pro Asp Ser Giu Phe 1564 WO 01/00843 WO 0100843PCTIBOO00923 <210> <211> <212> <213> <220> <221> <222> <223> 805 1145 1953

DNA

Corynebacterium

CDS

(101)..(1930) RXA02 645 giutamicun <400> 1145 gatacagctc cttgatggag tgaataaatt cgcgagcctg ctcctgatct tgcacacgcg tgatataggt cagaaatcgc gagcgcttga tctctagttc atg Met 1 ctc aaa gac Leu Lys Asp acc ggc ctg agg Thr Gly Leu Arg gag Glu ttg gta ttg cgt Leu Val Leu Arg atg tgc cat agc Met Cys His Ser atc tca Ile Ser cat ctt agc His Leu Ser ttg acc tcg Leu Thr Ser tcg cca acc ggc agc att ttc act agc ctg Ser Pro Thr Gly Ser Ile Phe Thr Ser Leu 30 caa agc ttt tca gtg tgg gct cca ctt ccc Gin Ser Phe Ser Val Trp Ala Pro Leu Pro gtg gcc atg Val Ala Met cac gat gta His Asp Val cat ctg His Leu atc ctc aac ggc Ile Leu Asn Gly acc ctc ccc atg Thr Leu Pro Met aaa acg gag ggc Lys Thr Giu Gly agc Ser tgg tgg cgc gcc Trp Trp Arg Ala gag Giu atc gcg ccc aag Ile Ala Pro Lys ggc gat cgt tac ggt Gly Asp Arg Tyr Gly ttt tcg ctt ttc Phe Ser Leu Phe ggc tcc tcc tgg Giy Ser Ser Trp aaa acc ctc ccc Lys Thr Leu Pro gat ccc Asp Pro 100 cgc tcc aca Arg Ser Thr gat gat tcc Asp Asp Ser 120 caa cca gac ggg Gin Pro Asp Gly gtt Val1 110 cat ggt tta agt His Gly Leu Ser gaa gtc tcc Giu Val Ser 115 cga att ctc Arg Ile Leu 403 451 499 tat ctg tgg ggt Tyr Leu Trp Gly gac Asp 125 cag cag tgg act Gin Gin Trp Thr cct ggc Pro Gly 135 tcg gtg tta tat Ser Vai Leu Tyr gag Giu 140 ctg cat gtg ggc Leu His Val Giy ttt agt gaa gat Phe Ser Giu Asp gga Gly 150 acg ttt gag gga Thr Phe Giu Giy gtc Val 155 gtc gac aag ctt Val Asp Lys Leu cc t Pro 160 tat ctg cgc gac Tryr Leu Arg Asp ggc gtg acc gcc atc gaa ctt tta ccc gtg cag ccc ttt ggc ggc aac 1565 WO 01/00843 Gly Val Tlh cgc aat tg Arg Asn TZ tac ggc g Tyr Gly GI 2C gcc ggc at Ala Gly Il 215 gac ggc aa Asp Gly As 230 acc ggc tq Thr Gly Tx gtc cgc aa Val Arg As cac gtt ga His Val As 2E ggc gcc ta 1027 Gly Ala Ty 295 tcc gca ca 1075 Ser Ala GI 310 aat gac cc 1123 Asn Asp Pz gat gca ca 1171 Asp Ala GI tct ggc ga 1219 Ser Gly GI 3E tta gcc aa 1267 Leu Ala Lj 375 Leu ggg Gly ttg Leu tta Leu 220 caa Gin gtC Val gac Asp ctc Leu gcg Ala 300 cca Pro acc Thr ga t Asp tat Tyr gaa Giu 380 Leu gtg Val1 aaa Lys 205 gac Asp tt t Phe aac Asn gcc Ala gat Asp 285 cag Gin cgc Arg tcc Ser atc Ile tac Tyr 365 gta Val1 Phe gtC Val1 gcc Ala 210 cac His tct Ser ga t Asp ttc Phe ctc Leu 290 gcc Ala gaa Giu ggt Gly cat His tct Ser 370 gga Gly Giy cat His 195 tcc Ser ttc Phe ggc Gly tca Ser gaa Giu 275 ga t Asp gag Glu tc t Se r tt t Phe gcc Ala 355 gtc Val1 aac Asn PCTIIBOO/00923 Giy Asn 180 gca ggc 691 Ala Gly cac cag 739 His Gin ggc ccc 787 Gly Pro ggc agc 835 Gly Ser 245 gat gaa 883 Asp Giu 260 gat ttt 931 Asp Phe gat cgc 979 Asp Arg gat gtc Asp Val gaa ctc Giu Leu 325 ggc ctg Gly Leu 340 ctc gtt Leu Vai gac aca Asp Thr tac tcc Tyr Ser 1566 WO 01/00843 WO 0100843PCTIBOO/00923 gag ctg att tac agc ttc act tcc ccc acc gtc acc gac acc tcc aca 1891 Glu Leu Ile Tyr Ser Phe Thr Ser Pro Thr Val Thr Asp Thr Ser Thr 585 590 595 acc ctt cag ccg tgg ggc ttt gcg atc ctg acc cga aac tagaaaaagg 1940 Thr Leu Gin Pro Trp Gly Phe Ala Ile Leu Thr Arg Asn 600 605 610 ccacctcgat tga 1953 <210> 1146 <211> 610 <212> PRT <213> Corynebacterium giutamicum <400> 1146 Met Leu Lys Asp Leu Thr Giy Leu Arg 1 Cys Ser Leu His Gly Thr Leu Thr Thr 145 Tyr Pro Ala Ser Val His Thr Arg Pro Giu 115 Arg Ser Arg Gly His 195 5 Ser Met Val Gly Giy Pro Ser Leu Asp Leu 165 Asn Gly His Leu His Ser 70 Phe Arg Asp Pro Gly 150 Gly Arg Tyr Ser Gin Leu Arg Phe Ser 105 Tyr Val1 Giu Ala Giy 185 Pro Giu 10 Pro Ser Asn Ala Asp 90 Gin Leu Leu Gly Ile 170 Tyr Al a Val1 Val Leu Gly Ser Ser Vai Giu Thr Ile Ala Ser Ser Asp Gly Gly Asp 125 Giu Leu 140 Val Asp Leu Leu Gly Val Leu Lys 205 Leu Asp 220 Arg Ile Trp Leu Pro Trp Val 110 Gin His Lys Pro Leu 190 Lys Val1 Giu Phe Ala Pro Lys Ser His Gin Val1 Leu Val1 175 Trp Leu Val Met Thr Pro Met Ala Lys Gly Trp Gly Pro 160 Gin His Ile Tyr Asp Ala Ser His Gin Ala Gly Ile Ala 210 215 1567 WO 01/00843 WO 0100843PCT/IBOO/00923 acg tac 1315 Thr Tyr 390 cct gcc 1363 Pro Ala aac cgc 1411 Asn Arg cag gtg 1459 Gin Val ttg ttt 1507 Leu Phe 455 tgc tcc 1555 Cys Ser 470 aaa cgg 1603 Lys Arg ccc gag 1651 Pro Glu act gcg 1699 Thr Ala ctg cgg 1747 Leu Arg 535 gtt gag 1795 Val Giu 550 att ctg 1843 Ile Leu cgc gga cgc aac Arg Gly Arg Asn 395 tcg cgc ttt gtc Ser Arg Phe Val 410 cac ggc cgc Hius Gly Arg acc tac acc Thr Tyr Thr cct gtg Pro Val 400 cac ccc gat atc acc His Pro Asp Ile Thr 405 gca atc Ala Ile 425 ttg aag Leu Lys ggc gac cgt cct tcc Gly Asp Arg Pro Ser 430 gca gcc att atc tac Ala Ala Ile Ile Tyr 445 gaa gaa ttc gga gcc Giu Giu Phe Gly Ala acc acc cat gat cag acc 99C Thr Thr His Asp Gin Thr Gly 415 420 acg act ctc acc ccg gaa cag Thr Thr Leu Thr Pro Glu Gin 435 agc tcg ccg tat acc ccg atg Ser Ser Pro Tyr Thr Pro Met 450 acc acc cca ttc gcc ttc ttt Thr Thr Pro Phe Ala Phe Phe ggt Gly 460 465 cac acc gac ccc His Thr Asp Pro 475 gaa ttc gca cgc Giu Phe Ala Arg gag ctc aac cgg Giu Leu Asn Arg ctt ggc tgg aac Leu Gly Trp, Asn c ta Leu 480 acc tcc Thr Ser gag ggc cgc Giu Gly Arg 485 ctg gaa Leu Giu 505 gag cag Glu Gin acc ttc acc tcc Thr Phe Thr Ser 510 cgc atc aac gac Arg Ile Asn Asp gcc gac gac atc ccc tcc Ala Asp Asp Ile Pro Ser 500 aaa ctc gat tgg gag ttc Lys Leu Asp Trp Giu Phe 515 tac aag cag ctg ttg cac Tyr Lys Gin Leu Leu His cgc Arg gct Ala 525 acc ttg ggc ttc Thr Leu Gly Phe 540 tcc Ser caa Gin 530 cca aac ttg ctc aca ctc gag Pro Asn Leu Leu Thr Leu Giu 545 cac ggc gag aac His Giy Glu Asn 555 gcg aat ttc tcc Ala Asn Phe Ser 570 tgg cta tcg atg gcc Trp Leu Ser Met Ala 560 gac gac acc atc acc Asp Asp Thr Ile Thr 575 aat ggt cgc ggc cga Asn Gly Arg Gly Arg 565 gtc ccg ctt ggc ggc Val Pro Leu Giy Giy 580 1568 WO 01/00843 WO 0100843PCTIBOOIOO923 Asn 225 Thr His Trp Ser Val 305 Ala Gly Leu Gly Thr 385 His His Leu Pro Pro 465 Thr Asp Leu Lys His Ser Asp Phe Leu 290 Ala Giu Gly His Ser 370 Gly Pro Asp Thr Tyr 450 Phe Ser Asp Asp Gin 530 Pro Ser 245 Glu Phe Arg Val1 Leu 325 Leu Val1 Thr Ser Thr 405 Gly Gin Met Phe Arg 485 Ser Phe His Asn Trp Asn Asp 280 Tyr Gin Pro Gin Glu 360 Lys Arg Ser Al a Leu 440 Met His Glu Leu Glu 520 His Gly Tyr 265 Gly Ser Thr Lys Trp 345 Arg Thr Gly Arg Ile 425 Lys Gly Thr Phe Giu 505 Gin Thr Asn Asp 250 Ile Leu Leu Gly Phe 330 Val Asn Leu Arg Phe 410 Giy Aia Giu Asp Aia 490 Ser Arg Leu Gly Ile Aia 270 Al a Leu Ser Arg His 350 Ser Phe Arg Thr Ser 430 Tyr Aia Asn Trp Ser 510 Asp Gin Pro Asn 255 Arg Val1 Thr Leu Glu 335 His Asp Glu Pro Thr 415 Thr Ser Thr Arg Asn 495 Ser Ala Pro Tyr 240 Giy Gin His Met Ile 320 Al a Ala Phe His Val1 400 Thr Thr Ser Thr Leu 480 Al a Lys Tyr Asn 1569 WO 01/00843 PCTIBOO/00923 Leu Leu Thr Leu Giu Val Giu His Gly Giu Asn Trp Leu Ser Met Ala 545 550 555 560 Asn Gly Arg Gly Arg Ile Leu Ala Asn Phe Ser Asp Asp Thr Ile Thr 565 570 575 Val Pro Leu Gly Gly Giu Leu Ile Tyr Ser Phe Thr Ser Pro Thr Val 580 585 590 Thr Asp Thr Ser Thr Thr Leu Gin Pro Trp Gly Phe Ala Ile Leu Thr 595 600 605 Arg Asn 610 <210> 1i47 <211> 832 <212> DNA <213> Corynebacterium giutamicun <220> <221> CDS <222> (i01)..(832) <223> RXN02355 <400> 1147 atttttgacc ctccgggggt gatttaacct aaaattccac acaaacgtgt tcgaggtcat tagattgata agcatctgtt gttaagaaag gtgacttcct atg tcc tcg att tcc 115 Met Ser Ser Ile Ser 1 cgc aag acc ggc gcg tca ctt gca gcc acc aca ctg ttg gca gcg atc 163 Arg Lys Thr Gly Ala Ser Leu Ala Ala Thr Thr Leu Leu Ala Ala Ile 15 gca ctg gcc ggt tgt agt tca gac tca agc tcc gac tcc aca gat tcc 211 Ala Leu Ala Gly Cys Ser Ser Asp Ser Ser Ser Asp Ser Thr Asp Ser 30 acc gct agc gaa ggc gca gac agc cgc ggc ccc atc acc ttt gcg atg 259 Thr Ala Ser Giu Gly Ala Asp Ser Arg Gly Pro Ile Thr Phe Ala Met 45 ggc aaa aac gac acc gac aaa gtc att ccg atc atc gac cgc tgg aac 307 Gly Lys Asn Asp Thr Asp Lys Val Ile Pro Ile Ile Asp Arg Trp, Asn 60 gaa gcc cac ccc gat gag cag gta acg ctc aac gaa ctc gcc ggt gaa 355 Giu Ala His Pro Asp Giu Gin Val Thr Leu Asn Giu Leu Ala Gly Giu 75 80 gcc gac gcg cag cgc gaa acc ctc gtg caa tcc ctg cag gcc ggc aac 403 Ala Asp Ala Gin Arg Giu Thr Leu Val Gin Ser Leu Gin Aia Gly Asn 95 100 tct gac tac gac gtc atg gcg ctc gac gtc atc tgg acc gca gac ttc 451 Ser Asp Tyr Asp Val Met Ala Leu Asp Val Ile Trp Thr Ala Asp Phe 105 110 115 1570 WO 01/00843 PCTIBOO/00923 ta aac 499 gcg Ala acc Thr acc Thr 150 aac Asn gaa Glu cag Gin gaa Giu gca Ala tcc Ser 135 ctc Leu acc Thr tcc Ser ctc Leu ggt Gly 215 aac Asn 120 gga Gly t ac Tyr gaa Giu tgc Cys aag Lys 200 tgg Trp gca Ala tcc Ser 140 cag Gin gaa Giu gaa Glu ggc Gly gtc Val 220 cca, Pro 125 acc Thr aac Asn gca Ala gaa.

Glu ct t Leu 205 cta Leu ctt Leu gtg Val acc Thr cca Pro gca Ala 190 t ca Ser gac Asp aga cag cac gac ggc aag gca ggc ctt Arg Gin His Asp Gly Lys Ala Gly Leu 230 235 <210> 1148 <211> 244 <212> PRT <213> Corynebacteriun glutamicum <400> 1148 Met Ser Ser Ile Ser Arg Lys Thr Gly 1 Leu Leu Ala Ala Ile Ala Leu Ala Gly 25 Asp Ser Thr Asp Ser Thr Ala Ser Giu 40 Ile Thr Phe Ala Met Gly Lys Asn Asp 55 Ile Asp Arg Trp Asn Giu Ala His Pro 70 Giu Leu Ala Gly Giu Ala Asp Ala Gin Leu Gin Ala Gly Asn Ser Asp Tyr Asp 100 105 Trp Thr Ala Asp Phe Ala Ala Asn Gin 1571 caa Gin ggC Gly tcc Ser ggc Gly 160 aac Asn gtt Val aac Asn gac Asp gcg Ala 240 Ser Ser Ala Asp Giu Giu Met Leu gac Asp gca Ala 145 cag Gin tgg Trp gat Asp acc Thr ggC Gly 225 ctt Leu Leu Ser Asp Lys Gin Thr Ala Ala ctc Leu 130 acc Thr cta Leu gct Ala tgc Cys atc Ile 210 aaa Lys gtc Val Ala Asp Ser Val1 Val1 Leu T.eu Pro ctg ttc Leu Phe gac ctc Asp Leu 180 ctg acc Leu Thr 195 ggc ttc Gly Phe cgt cac Arg His gac ggc Asp Gly gag Giu tac Tyr cgc Arg 165 gtg Val act Thr atc Ile cgt Arg Ala Ser Arg Ile Thr Val Asp 110 Leu WO 01/00843 115 Asp Leu Giu 130 Ala Thr Tyr 145 Gin Leu Leu Trp, Ala Asp Asp Cys Leu 195 Thr Ile Giy 210 Giy Lys Arg 225 Leu Vai Asp PCTIBOO/00923 Asp Giy Arg 165 Val1 Thr Ile Arg Thr Thr 150 Asn Giu Gin Giu Arg 230 Ser 135 Leu Thr Ser Leu Giy 215 Gin 120 Giy Giu Cys Lys 200 Trp His Leu Aia Ile Thr 185 Gin Giy Asp Gin Pro 155 Pro Aia Giu Ser Lys 235 Ser 140 Gin Giu Giu Giy Vai 220 Ala Asp Asn Aia 175 Giy Vali Asp Gin Ser Giy 160 Asn Val Asn Asp Ala 240 <210> 1149 <211> 609 <212> DNA <213> Corynebacteriun giutamicun <22 0> <221> CDS <222> (101)..(586) <223> RXN02909 <400> 1149 caacgcgaat gaaaacgaac agcgagcagg tctataccca atagtttcct ggattgtttg gcacagtcgg gagaaaactc atc Ile ggt Gly att Ile tcc Ser ggC gtt Val gaa Glu gat Asp ga t Asp acg ctt Leu atg Met gag Glu ttt Phe ggg ctt Leu gat Asp aat Asn aat Asn gcg 1572 cgacgtcaac gtgtgggcta atg aac cgc gca cga Met Asn Arg Aia Arg 1 ctg ctg gcg tcc tgt Leu Leu Aia Ser Cys tgg ttg gtg acc aat Trp, Leu Vai Thr Asn agt aat ctt gtc att Ser Asn Leu Vai Ile ctg tct ggt ttc act Leu Ser Giy Phe Thr ttc caa aat ggt gag 115 163 211 259 307 355 WO 01/00843 Gly Cys Va caa agc tc Gin Ser Se ttc gat aa Phe Asp Ly .1 Pro Phe t gtt ctg Val Leu *a ctt ccc s Leu Pro 105 Arg gat Asp tgc Cys t

C

C

1 la :at .yr :aa 1n .10 jgt 1y 'gc ~er Ltc le Giu gtg Val 95 gga Gly tct Ser gat Asp gcg Ala Gin tc t Ser ctc Leu atc Ile 130 gaa Giu tct Ser Asn tcc Ser aaa Lys 115 tcc Ser PCTIBOO/00923 iy Giu tg gat 403 eu Asp 00 tt cat 451 al His gg act 499 rci Thr aac gag ctg gtt gat cLL ctg cct C Asn Giu Leu Vai Asp Leu Leu Pro C 120 125 tct ggt tca gaa atc ttg ctg act a Ser Giy Ser Glu Ilie Leu Leu Thr 135 140 cca gca atc cgc ttg gtg tcc tgg z Pro Ala Ilie Arg Leu Val Ser Trp 1 150 -155 gggctttaaa gtg <210> 1150 <211> 162 <212> PRT <213> Corynebacteriun glutamicun ctc gat cgg Leu Asp Arg taaggtgcca <400> 1i50 Met Asn Arg Ala Ile Ala Thr Ile 1 Leu Trp Ser Leu Phe Leu Giu Giu Asp 145 Leu Leu Asn Ser Gin Ser Leu Ile 130 Glu Gly Ile Ser Gly 70 Gin Phe Asn Ser Pro 150 Ser Tyr Gin Cys Ser Asp Glu Giy 135 Ala Thr Asp Ser Pro Vai Leu 105 Vai Glu Arg Gly 10 Val Pro Leu Phe Leu Pro Asp Ile Leu Vali Giu Asp Asp Thr 75 Asp Asp Leu Leu Val1 155 Pro Thr Ser Giy Arg Asp Cys Pro 125 Thr Trp, Leu Asp Asn Asn Ala Tyr Gin 110 Gly Ser Ile Ala Ser Ser Ser Giu Vai Giy Ser Asp Ala Leu Thr Ile Ser Phe Thr Gin Phe Val1 Pro 160 1573 WO 01/00843 WO 0100843PCTIiBOO/00923 Thr Ser <210> 1151 <211> 1590 <212> DNA <213> Corynebacterium <220> <221> CDS <222> (101)..(1567) <223> RXS00349 <400> 1151 tgtgtacatc acaatggaat giutamicum tcggggctag agtatctggt gaaccgtgca taaacgacct gtgattggac tctttttcct tgcaaaatgt tttccagcgg atg ttg agt ttt gcg Met Leu Ser Phe Ala acc ctt cgt ggc Thr Leu Arg Gly att tca aca gtt Ile Ser Thr Val gct gca aaa gcc Ala Ala Lys Ala gca cct Ala Pro ccg cca tcg Pro Pro Ser cta gcc ccg att Leu Ala Pro Ile ctc act gac cat Leu Thr Asp His agt caa gtg Ser Gin Vai ttg ctt tct Leu Leu Ser gcc ggt gtg atg aat ttg gct gcg aga att ggc gat Ala Gly Val Met Asn Leu Ala Ala Arg Ile Giy Asp tca ggt Ser Gly acg tca aat agt Thr Ser Asn Ser gac Asp acc aag gta caa Thr Lys Val Gin cga gca gtg acc Arg Ala Val Thr tct Ser gcg tac ggt ttg Ala Tyr Gly Leu tac acg cac gtg Tyr Thr His Val atc acg ttg aat Ile Thr Leu Asn atc acc atc ttc Ile Thr Ile Phe aac atc ggt gtg Asn Ile Gly Val agg aag atg ccg Arg Lys Met Pro gtc aac Val Asn 100 gtg ttt cat gtt gta ggc aag ttg Val Phe His Val Val Gly Lys Leu 105 gac Asp 110 acc aac ttc tcc Thr Asn Phe Ser aaa ctg tct Lys Leu Ser 115 acc ccg cct Thr Pro Pro gag gtt gac Glu Val Asp 120 cgt ttg atc cgt Arg Leu Ile Arg tcc Ser 125 att cag gct ggt Ile Gin Ala Gly gag gtt Giu Val 135 gcc gag aaa atc ctg gac gag ttg gag caa tcc cct gcg tct Ala Giu Lys Ile Leu Asp Giu Leu Giu Gin Ser Pro Ala Ser tat Tyr 150 ggt ttc cct gtt Gly Phe Pro Val gcg Ala 155 ttg ctt ggc tgg Leu Leu Gly Trp gca Al a 160 atg atg ggt ggt Met Met Gly Gly gc t Al a 165 1574 WO 01/00843 WO 0100843PCTIIBOO/00923 gtt gct Val Ala att acc Ile Thr ggt ttg Gly Leu ctg cct Leu Pro 215 atc aaa Ile Lys 230 ggt ttg Gly Leu gtg aca Val Thr att gtt Ile Val gtc atg 1027 Val Met 295 aca ttc 1075 Thr Phe 310 gtg ggt 1123 Val Gly gcg ctg 1171 Ala Leu ccc gtc 1219 Pro Val ggt ttg 1267 gtg ctg ttg Val Leu Leu 170 gcg ttc acg Ala Phe Thr 185 cct act ttc Pro Thr Phe 200 gca tcg att Ala Ser Ile ccg agc cag Pro Ser Gin aca ctc gtg Thr Leu Val 250 gca agt gca Ala Ser Ala 265 gct ggc gtg Ala Gly Val 280 ttg cct gcc Leu Pro Ala gcc cgc att Ala Arg Ile tgt tac gcg Cys Tyr Ala 330 atg ggt tct Met Gly Ser 345 tct gcc gct Ser Ala Ala 360 ctt gcc cgt ggt ggt gga tgg cag gtt tcC cta att Gly Gly Gly Trp Gin Val Ser Leu Ile 175 att Ile caa Gin tat Tyr 220 atc Ile tct Ser t tt Phe ttg Leu gag Glu 300 gc t Ala tgg Trp ttt Phe att Ile ttc gcc Ala aa t Asn 205 tct Ser gca Ala ctg Leu ttc Phe ggc Gly 285 tcc Ser ggt Gly tcc Ser tat Tyr gc t Al a 365 ttg gct Ala 180 aag Lys gcc Ala ctt Leu ttg Leu gc t Al a 260 ggc Gly ttg Leu tcg Ser t tc Phe ctt Leu 340 tta Leu act Thr gog ttt Phe aag Lys acg Thr gag Glu gca Ala 245 ccg Pro 99c Gly cat His tct Ser gca Al a 325 act Thr ggC Gly ggt Gly att Gly Leu Leu Ala Arg Arg Phe Leu Ile Pro Pro Leu Ile Val Ala Ile 1575 WO 01/00843 375 gcc ggc at 1315 Ala Gly Il 390 tac gcc ac 1363 Tyr Ala T1 gtt gct tt 1411 Val Ala LE gag tgg at 1459 Glu Trp IJ cgt gca tt 1507 Arg Ala P1 455 cag aat ca 1555 Gin Asn G] 470 ggt aat az 1590 Gly Asn Lj PCTIBOO/00923 c e

C

r a u t e 0 t e 9 n a s aca cca atg Thr Pro Met 395 ctg aat gat Leu Asn Asp 410 gcc act gct Ala Thr Ala 425 gcc cgc agg Ala Arg Arg acc aag gcg Thr Lys Ala cgc cgg cag Arg Arg Gin 475 380 ctt cca ggt cta Leu Pro Gly Leu caa aca ctc atg Gin Thr Leu Met 415 tca tca ctt gcc Ser Ser Leu Ala 430 cta cgt cgt cca Leu Arg Arg Pro 445 aat gag ttc tcc Asn Giu Phe Ser 460 aga aaa cgt cca Arg Lys Arg Pro gca Ala 400 gg t Gly gc t Ala cca Pro t tc Phe aag Lys 480 385 att Ile ttC Phe ggC Gly cgc Arg cag Gin 465 act Thr tac Tyr acc Thr gtg Val1 ttC Phe 450 gag Glu aat Asn atg Met 405 gcg Al a ggt Gly tac Tyr gag Glu ttc Phe 485 agg Arg taaaaatcaa cctgcttagg cgt <210> 1152 <211> 489 <212> PRT <213> Corynebacteriun glutamicum <400> 1152 Met Leu Ser Phe Ala Thr Leu Arg( 1 Ala Lys Ala Ala Pro Pro Pro Ser 1 Asp His Ser Gin Val Ala Gly Val 1 Asp Ile Leu Leu Ser Ser Gly Thr! 55 Val Arg Ala Val Thr Ser Ala Tyr 70 Ile Thr Leu Asn Thr Ile Thr Ile 1 Ser Pro Ala Asp Tyr Ile Al a Thr Gly Gin Asp Arg 1576 WO 01/00843 WO 0100843PCTJLBOOIOO923 Lys Phe Giy Gin 145 Met Ser Phe Giy Gin 225 Val Ile Leu Ser Pro 305 Ala Ile Val Vali Leu 385 Met Ser Ala 130 Ser Met Leu Leu Phe 210 Phe Vai Thr Phe Glu 290 Asn Ala Ala Val1 Giy 370 Ile Pro Lys 115 Thr Pro Giy Ile Giy 195 Ile Gly Leu Giy Thr 275 Ile Tyr Al a Gly Tyr 355 Phe Val1 Val Asn Val Phe His Val Val Giy Lys Leu 100 105 Asp Thr Asn 110 Leu Pro Ala Giy Ala 180 Lys Aia Leu Leu Ala 260 Gly Leu Ser Phe Leu 340 Leu Thr Ala Ser Pro Ser Ala 165 Phe Lys Thr Giu Ala 245 Pro Gly His Ser Ala 325 Thr Gly Gly Ile Glu Glu Tyr 150 Val Ile Gly Leu Ile 230 Gly Val1 Ile Val1 Thr 310 Val1 Ala Pro Gly Ala 390 Val Val 135 Gly Ala Thr Leu Pro 215 Lys Leu Thr Val Met 295 Phe Gly Leu Vali Leu 375 Gly Asp Arg 120 Ala Giu Phe Pro Vai Leu Ala Phe 185 Pro Thr 200.

Ala Ser Pro Ser Thr Leu Aia Ser 265 Aia Giy 280 Leu Pro Ala Arg Cys Tyr Met Gly 345 Ser Aia 360 Leu Ala Ile Thr Leu Lys Val Leu 170 Thr Phe Ile Gin Val 250 Ala Val Ala Ile Al a 330 Ser Aia Arg Pro Asn 410 Ile Ile Al a 155 Gly Ile Phe Ala Ile 235 Gin Arg Gly Met Ile 315 Glu Aia Ala Arg Met 395 Asp Arg Leu 140 Leu Gly Ile Gin Tyr 220 Ile Ser Phe Leu Glu 300 Ala Trp Phe Ile Phe 380 Leu Gln Ser 125 Asp Leu Gly Ala Asn 205 Ser Ala Leu Phe Gly 285 Ser Gly Ser Tyr Aia 365 Leu Pro Thr Ile Glu Gly Trp Thr 190 Val1 Leu Ser Gin Giu 270 Ile Ala Gly Ser Tyr 350 Ala Ile Gly Leu Gin Leu Trp Gin 175 .Thr Vali Ala Gly Asp 255 Thr Gin Al a Val1 Val1 335 Ldu Thr Pro Leu Met 415 Ala Giu Ala 160 Val1 Ser Gly Leu Ile 240 Gly Leu Leu Ala Thr 320 Ile Phe Ala Pro Aia 400 Gly Ile Tyr Arg Gly Met Tyr Ala Thr Leu 405 1577 WO 01/00843 Phe Thr Asn Gly Val Val 435 Arg Phe Asn 450 Gin Giu Glu 465 Thr Asn Gin Ala Gly Tyr Giu Phe 485 PCTIBOO/00923 Val Ala Leu Ala Thr Ala Ser Ser Leu Ala Ala 425 430 Giu Trp Ile Ala Arg Arg Leu Arg Arg Pro Pro 440 445 Arg Ala Phe Thr Lys Ala Asn Glu Phe Ser Phe 455 460 Gin Asn Gin Arg Arg Gin Arg Lys Arg Pro Lys 470 475 480 Gly Asn Lys Arg <210> 1153 <211> 440 <212> DNA <213> Corynebacteriun giutaiicun <220> <221> CDS <222> .(417) <223> RXS03183 <400> 1153 gaa.gcc gaa Glu Ala Giu 1 aaa gac ggc Lys Asp Gly aac gtc aac Asn Val Asn atc atc aac Ile Ile Asn cca gtt ctg Pro Val Leu cca tac ctg Pro Tyr Leu cca gtg tct Pro Val Ser gcc tac gca Ala Tyr Ala 115 gat atg aag gca Ala gta Val aac Asn aac Asn atc Ile 70 c tg Leu tac Tyr aac Asn atc ggC Gly tcc Ser aag Lys caa Gin tac Tyr aag Lys cca Pro ggC Gly gaa ttc Phe ctt Leu acc Thr tgg Trp gat Asp tcc Ser atc Ile 105 gtc Val gct gaa Glu 10 gg t Gly gcc Ala ttc Phe gag Glu ctg Leu tcc Ser gac Asp ccc Pro aac Asn t tc Phe aac Asn gtt Val1 gca Ala atc Ile cag ctc Leu aac Asn atc Ile tcc Ser gag Giu gca Ala cag Gin 110 gca gta Val1 ggC Gly gaa Giu t tc Phe cag Gin cca Pro gac Asp ac c ggt Gly atc Ile ttc Phe cca Pro tac Tyr cgc Arg aac Asn acc 48 96 144 192 240 288 336 384 Asp Gin Ala Thr Thr 125 tcc agc tagttcggta atttagttca 437 Asp Met Lys Ala Ala Ile Giu Asn Ala Ser Ser 1578 WO 01/00843 PCTIIBOOIOO923 130 135 ttC 440 <210> 1154 <211> 139 <212> PRT <213> Corynebacterium giutanicun <400> 1154 Glu Ala Giu Ala Thr Ala Gly Lys Phe Glu Val Gin Pro Leu Val Gly 1 5 10 Lys Asp Gly Val Gly Val Ser Thr Leu Gly Gly Tyr Asn Asn Giy Ile 25 Asn Val Asn Ser Glu Asn Lys Ala Thr Ala Arg Asp Phe Ile Glu Phe 40 Ile Ile Asn Giu Giu Asn Gin Thr Trp Phe Ala Asp Asn Ser Phe Pro 55 Pro Val Leu Ala Ser Ile Tyr Asp Asp Giu Ser Leu Val Glu Gin Tyr 70 75 Pro Tyr Leu Pro Ala Leu Lys Glu Ser Leu Glu Asn Ala Ala Pro Arg 90 Pro Val Ser Pro Phe Tyr Pro Ala Ile Ser Lys Ala Ile Gin Asp Asn 100 105 110 Ala Tyr Ala Ala Leu Asn Gly Asn Val Asp Val Asp Gin Ala Thr Thr 115 120 125 Asp Met Lys Ala Ala Ile Giu Asn Ala Ser Ser 130 135 <210> 1155 <211> 1212 <212> DNA <213> Corynebacterium glutamicum <220> <221> CDS <222> (1189) <223> RXC00874 <400> 1155 agctgttccc taccattgct gaacgggagt ggattgtcac tttagcccct cacggattct tctggtttga tctcaccgcc gatgaaaagg acgatatgga atg agc att ggc caa 115 Met Ser Ile Gly Gin 1 cac atc atc acc gag cgt ttc tac ggc gcc aag tcc cac acc atc gac 163 His Ile Ile Thr Glu Arg Phe Tyr Gly Ala Lys Ser His Thr Ile Asp 15 aac gta gat att gtg ttg tcc cgc gaa tgt ggc gag aac act ttg gct 211 1579 WO 01/00843 Asn Val Asp gta gtg cgc Val Val Arg ggc aaa gat Gly Lys Asp gga gca tgg Gly Ala Trp ctc ggc aca Leu Gly Thr gcg atc gtg Ala Ilie Val gtg gag cta Val Giu Leu 120 ctg ggt ttt Leu Gly Phe 135 atg gcg cag Met Ala Gin 150 act act acc Thr Thr Thr gcc acc cgc Ala Thr Arg gta gtt ccc Val Val Pro 200 gaa cta atc Giu Leu Ile 215 atc gac ctc Ile Asp Leu 230 cac ggt gac His Gly Asp Ile atc Ile gtt Val act Thr gaa Glu aaa Lys 105 att Ile t cc Ser cag Gin tc t Ser aa t Asn 185 gta Val1 tcc Ser tac Tyr ctc Leu Val1 aac Asn ctc Leu ggc Gly caa Gin tac Tyr tct Ser t cc Ser tac Tyr ggc Gly 170 gtt Vai gaa Giu caa Gin caa Gin cac His 250 Leu aat Asn aac Asn agc Ser 75 tcc Ser ttc Phe aa a Lys gct Al a gt t Val 155 agc Ser cac His gca Ala gca Ala tcg Ser 235 t tg Leu Ser gcg Al a gac Asp 60 tct Ser aat Asn cgc Arg att Ile gag Giu 140 cca Pro t tt Phe act Thr ctc Leu ccc Pro 220 t tg Leu ggg Gly ggc Gly Arg Giu Cys 30 ctg tat cag Leu Tyr Gin 45 cac gta gaa His Val Giu gct ttt ccc Ala Phe Pro agc tct ttc Ser Ser Phe 95 aaa tta gaa Lys Leu Giu 110 tcc tcc tgc Ser Ser Cys 125 atc tcc ggg Ile Ser Gly ggt ttg gat Gly 14eu Asp gca gag gat Ala Giu Asp 175 gct ctt gca Ala Leu Ala 190 gcc gat gcg Ala Asp Ala 205 gaa atc gcc Giu Ile Ala gaa ggc gaa Giu Gly Glu cag ctc atc Gin Leu Ile 255 gaa cct gcc Giu Pro Ala Gly Glu ttg ttg Leu Leu gag gtc Giu Val att ggc Ile Gly 80 atc acc Ile Thr tcc ggg Ser Gly ccc aac Pro Asn gct aac Ala Asn 145 ggc tgg Gly Trp 160 gca gaa Ala Glu tcg gcc Ser Ala ctc act Leu Thr cgc ttc Arg Phe 225 gcc cac Ala His 240 aaa acc Lys Thr cgc cca Arg Pro Asn gtC Val ggt Gly cct Pro gcc Ala caa Gin atc Ile 130 t ac Tyr tca Ser aag Lys ttc Phe acc Thr 210 aaa Lys atc Ile ccc Pro ctt Leu Thr aa t Asn gcg Ala ttc Phe gac Asp aac Asn 115 gcg Ala acc Thr cac His atc Ile cct Pro 195 cgc Arg gaa Giu caa Gin gaa Glu aa t Asn PCTJIBOO/00923 Leu Ala gat gat 259 Asp Asp agt ttc 307 Ser Phe act cca 355 Thr Pro aat aaa 403 AsnLys 100 ccc gat 451 Pro Asp ccc atc 499 Pro Ile ctg gtc 547 Leu Val gcg ctg 595 Ala Leu 165 ggc gaa 643 Gly Giu 180 act cgg 691 Thr Arg ctt aat 739 Leu Asn gca gcc 787 Ala Ala cgc atc 835 Arg Ile 245 cgc tac 883 Arg Tyr 260 caa cga 931 Gin Arg atc ctc atc Ile Leu Ile gat ttc gaa Asp Phe Glu 1580 WO 01/00843 WO 0100843PCT/BOOIOO923 265 270 cgc ctc ccc gac tct ccc ctg aaa gat Arg Leu Pro Asp Ser Pro Leu Lys Asp 280 285 atc gac tac gca gcc tac ttc gac ggc 1027 Ile Asp Tyr Ala Ala Tyr Phe Asp Giy 295 300 gaa gcc acc gcg cta ttc ctc gac ggc 1075 Glu Ala Thr Ala Leu Phe Leu Asp Gly 310 315 gaa ctc ctc aat gcc tac att ctg gac 1123 Glu Leu Leu Asn Ala Tyr Ile Leu Asp 330 tat gaa ata aac aac cgc ccc gac tgg 1171 Tyr Glu Ilie Asn Asn Arg Pro Asp Trp 345 350 gtc gaa agg ctt cta gac tagttagtta 1212 Val Glu Arg Leu Leu Asp 360 <210> 1156 <211> 363 <212> PRT <213> Corynebacteriun glutanicun <400> 1156 Met Ser Ile Gly Gin His Ile Ile Thr 1 5 Ser His Thr le Asp Asn Val Asp Ile 25 Giu Asn Thr Leu Ala Val Val Arg Ile 40 Leu Val Asn Asp Asp Gly Lys Asp Val Val Gly Ala Ser Phe Gly Ala Trp Thr 70 Gly Pro Phe Thr Pro Leu Gly Thr Glu Thr Ala Asp Asn Lys Ala Ile Val Lys 100 105 Gly Gin Asn Pro Asp Val Glu Leu Ile 115 120 ctc gcc ggc atc Leu Ala Gly Ile 290 gaa cac acc caa Giu His Thr Gin 305 tac gga tca att Tyr Gly Ser Ile 320 aag gcg ttg tac Lys Ala Leu Tyr 335 gtg aaa atc cca Val Lys Ile Pro ctctgcgtca aac 275 atc Ile tgg Trp gaa Glu gag Glu c tc Leu 355 Gly Glu Tyr Val1 Phe Ser Leu 110 Ser aga Arg gcc Al a gac Asp gt t Val1 340 gag Glu Al a Cys Gin Glu Pro Phe Glu Cys tcc 979 Ser aac Asn caa Gin 325 gcc Ala gcg Ala Lys Gly Leu Glu Ile Ile Ser Pro Glu 10 Val1 Asn Leu Gly Gin 90 Tyr Ser Arg Leu Asn Asn Ser 75 Ser Phe Lys Tyr Arg Leu His Ala Ser Lys Ser 125 WO 01/00843 WO 0100843PCT/IBOO/00923 Asn Asn 145 Trp Giu Ala Thr Phe 225 His Thr Pro Gly Thr 305 Ser Leu Ile Ala Thr His Ile Pro 195 Arg Giu Gin Giu Asn 275 Ile Trp Giu Giu Leu 355 Pro Leu Ala Giy 180 Thr Leu Ala Arg Arg 260 Gin Arg Ala Asp Val 340 Glu Ile Val1 Leu 165 Giu Arg Asn Aia Ile 245 Tyr Arg *Ser Asn Gin 325 Ala Ala Leu Met 150 Thr Ala Vai Giu Ile 230 His Ile Arg Ile Giu 310 Glu Tyr Val1 Gly Phe 135 Ala Gin Thr Thr Thr Arg Vai Pro 200 Leu Ile 215 Asp Leu Gly Asp Leu Ile Leu Pro 280 Asp Tyr 295 Ala Thr Leu Leu Giu Ile Giu Arg 360 Ser Gin Ser Asn 185 Vai Ser Tyr Leu Asp 265 Asp Ala Ala Asn Asn 345 Leu Ser Tyr Gly 170 Val Glu Gin Gin His 250 Phe Ser Ala Leu Al a 330 Asn Leu Ala Vai i55 Ser His Ala Al a Ser 235 Leu Giu Pro Tyr Phe 315 Tyr Arg Asp Giu 140 Pro Phe Thr Leu Pro 220 Leu Gly Giy Leu Phe 300 Leu Ile Pro Ile Gly Ala Ala Al a 205 Giu Glu Gin Giu Lys 285 Asp Asp Leu Asp Ser Leu Giu Leu 190 Asp Ile Gly Leu Pro 270 Asp Gly Gly Asp Trp 350 G iy Asp Asp 175 Aia Ala Ala Giu Ile 255 Ala Leu Giu Tyr Lys 335 Val Ala Gly 160 Ala Ser Leu Arg Ala 240 Lys Arg Ala His Gly 320 Ala Lys 1582

Claims (30)

1. An isolated Corynebacterium glutamicum nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO;1, or a complement thereof.

2. An isolated nucleic acid molecule which encodes a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2, or a complement thereof.

3. An isolated nucleic acid molecule which encodes a naturally occurring allelic variant of a polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2, or a complement thereof.

4. An isolated nucleic acid molecule comprising a nucleotide sequence which 10 is functionally equivalent, and at least 50% identical, to the entire nucleotide sequence set forth in SEQ ID NO:1, or a complement thereof.

5. An isolated nucleic acid molecule comprising a fragment of at least contiguous nucleotides of the nucleotide sequence set forth in SEQ ID NO:1, or a complement thereof. 15 6. An isolated nucleic acid molecule comprising the nucleic acid molecule of S:any one of claims 1 to 5 and a nucleotide sequence encoding a heterologous S.polypeptide.

7. A vector comprising the nucleic acid molecule of any one of claims 1 to 6.

8. The vector of claim 7, which is an expression vector.

9. A host cell transfected with the expression vector of claim 8. The host cell of claim 9, wherein said cell is a microorganism.

11. The host cell of claim 10, wherein said cell belongs to the genus Corynebacterium or Brevibacterium. COMS ID No: SBMI-01718461 Received by IP Australia: Time 11:28 Date 2005-10-12 12/10 '05 WED 11:21 FAX 61 2 9888 7600 WATERMARK l00oo 149

12. The host cell of claim 9, wherein the expression of said nucleic acid molecule results in the modulation in production of a fine chemical from said cell.

13. The host cell of claim 12, wherein said fine chemical is selected from the group consisting of: organic acids, nonproteinogenic amino acids, purine and pyrimidine bases, nucleosides, nucleotides, lipids, saturated and unsaturated fatty acids, diols, carbohydrates, aromatic compounds, vitamins, cofactors, polyketides, and enzymes.

14. A method of producing a polypeptide, the method comprising culturing the host cell of claim 9 in an appropriate culture medium to, thereby, produce the polypeptide.

15. An isolated polypeptide comprising an amino acid sequence set forth in SEQ ID NO:2.

16. An isolated polypeptide comprising a naturally occurring allelic variant of a polypeptide comprising an amino acid sequence set forth in SEQ ID NO:2.

17. An isolated polypeptide which is encoded by a nucleic acid molecule comprising a nucleotide sequence which is functionally equivalent, and at least identical, to the entire nucleotide sequence set forth in SEQ ID NO:1.

18. An Isolated polypeptide comprising an amino acid sequence which is S functionally equivalent, and at least 51% identical, to the entire amino acid sequence set forth in SEQ ID NO;2.

19. An isolated polypeptide comprising a fragment of at least 20 contiguous amino acids of the amino acid sequence set forth in SEQ ID NO:2, wherein said polypeptide fragment maintains a biological activity of the polypeptide comprising the amino acid sequence set forth in SEQ ID NO:2.

20. The isolated polypeptide of any one of claims 15 to 19, further comprising heterologous amino acid sequences. COMS ID No: SBMI-01718461 Received by IP Australia: Time 11:28 Date 2005-10-12 12/10 '05 WED 11:22 FAX 61 2 9888 7600WAEAR j00 WATERMARK im 009 150

21. A method for producing a fine chemical, comprising culturing the cell of claim 9 such that the fine chemical is produced.

22. The method of claim 21, wherein said method further comprises the step of recovering the fine chemical from said culture.

23. The method of claim 21, wherein said cell belongs to the genus Corynebacterium or Brevibacterium.

24. The method of claim 21, wherein said cell is selected from the group consisting of: Cotynebacterium glutamicum, Corynebacterium herculis, Corynebacterium, Ilurn, Corynebactetium acetoacdophilum, Corynebacterium acetoglutamicum, Corynebacterium acetop hi/urn, Corynebacterium ammonia genes, Corynebacterium fujiokense, Corynebacterium nit ri/ophilus, Bre v/ba cterium ammonia genes, Brevibacterium butanicum, Brevibacterium divaricatum, Brevibacterlum flavurn, Brevibacterium heal4 Brevibacterium *ketioglutamicum, Orevibacterium ketosoreductum, Brevibacterium lactofermentum, Bre v/bacterium linens, Brevibacterium paraffinolyticum, and those strains set forth in Table 3, The method of claim 21, wherein expression of the nucleic acid molecule from said vector results in modulation of production of said fine chemical.

26. The method of claim 21, wherein said fine chemical Is selected from the group consisting of: organic acids, nonproteinogenic amino acids, purine and pyrimidine bases, nucleosides, nucleotides, lipids, saturated and unsaturated fatty acids, diols, carbohydrates, aromatic compounds, vitamins, cofactors, polyketides and enzymes.

27. The method of claim 21, wherein said fine chemical is an amino acid.

28. The method of claim 27, wherein said amino acid is drawn from the group consisting of: lysine, glutamate, glutarnine, alanine, aspartate, glycine, serine, COMS ID No: SBMI-01718461 Received by IP Australia: Time 11:28 Date 20D5-10-12 12/10 '05 WED 11:22 FAX 61 2 9888 7600 WATERMARK oio 151 threonine, methionine, cysteine, valine, leucine, isoleucine, arginine, proline, histidine, tyrosine, phenylalanine, and tryptophan.

29. A method for producing a fine chemical, the method comprising culturing a cell whose genomic DNA has been altered by the inclusion of a nucleic acid molecule of any one of claims 1 to 6. A method for diagnosing the presence or activity of Corynebacterium diphtheriae In a subject, the method comprising detecting the presence of at least one of the nucleic acid molecules of any one of claims 1 to 6 or the polypeptide molecules of any one of claims 15 to 20 in the subject, thereby detecting the presence or activity of Corynebacterium diphtheriae in the subject.

31. A host cell comprising a nucleic acid molecule comprising the nucleotide sequence set forth in SEQ ID NO:1, wherein the nucleic acid molecule is disrupted,

32. A host cell comprising a nucleic acid molecule comprising the nucleotide 15 sequence set forth in SEQ ID NO:1, wherein the nucleic acid molecule comprises one or more nucleic acid modifications as compared to the nucleotide sequence set forth in SEQ ID NO:1.

33. A host cell comprising a nucleic acid molecule comprising the nucleotide 0 *4 sequence set forth in SEQ ID NO:1, wherein the regulatory region of the nucleic S 20 acid molecule is modified relative to the wild-type regulatory region of the molecule,

34. A polypeptide produced by the method of claim 14. COMS ID No: SBMI-01718461 Received by IP Australia: Time 11:28 Date 2005-10-12 I 12/10 '05 WED 11:22 FAX 61 2 9888 7600WARAR01 WATERMARK [moil 152 A fine chemical produced by the method of any one of claims 21 to 29. DATED this 12th day of October 2005 BASF AKTIENGESELLSCHAFT1 WATERMARK PATENT TRADE MARK ATTORNEYS 290 BURWOOD ROAD HAWTHORN VICTORIA 3122 AUSTRALIA P20691 AUoo COMS ID No: SBMI-01718461 Received by IP Australia: Time 11:28 Date 2005-10-12