AU674525B2 - Biologically active peptides having N-terminal substitutions - Google Patents

Description

BIOLOGICALLY ACTIVE PEPTIDES HAVING

N-TERMNAL SUBSTITUTIONS

This invention relates to biologically active peptides. More particularly, this invention relates to biologically active peptides having N-terminal (or amino-terminal) substitutions.

In accordance with an aspect of the present invention, there is provided an N-terminal substituted peptide or protein having the formula: , wherein X is a biologically active

peptide or protein. The peptide or protein is preferably an ion channel-forming peptide or protein. T is a lipophilic moiety, and W is T or hydrogen.

The term "lipophilic," as used herein, means that the lipophilic moiety enhances the interaction of the peptide or protein with a lipid membrane, such as, for example, a cell membrane.

Lipophilic moieties which may be employed, include, but are not limited to, any moiety which may be placed on the N-terminal of the peptide through a condensation reaction with nitrogen. The lipophilic moiety T may be, for example, a carboxylic acid, a phosphoric acid, preferably an alkylphosphoric acid, a phosphonic acid, preferably an alkylphosphonic acid, a sulfonic acid, preferably an alkylsulfonic acid, or an alkyl group . Preferably, T is :

wherein R is a hydrocarbon having at least two

and no more than 16 carbon atoms.

In one embodiment, R is an alkyl group. The alkyl group may be a straight chain or branched chain alkyl group; or a cycloalkyl group. For example, R may be CH₃(CH₂)_n-, wherein n is from 1 to 14. Preferably, n is from 4 to 9, and most preferably n is 6, whereby T is an octanoyl group.

In another embodiment, R is an aromatic (including phenyl and naphthyl), or an alkyl aromatic group. For example, R may be 0 -(CH₂)_z-, wherein z is from 0 to 6.

Preferably, z is 1 or 2.

In one embodiment, W is hydrogen.

Applicant has found, that when biologically active peptides have substitutions at the N-terminal such as those hereinabove described, such peptides have increased biological activity against target cells, viruses, and virally-infected cells, as compared with unsubstituted peptides or peptides substituted at the N-terminal with an acetyl group. Applicant also has found that the N-terminal substitutions hereinabove described significantly increase the biological activity of "short" peptides, i.e. peptides having no more than 14 amino acid residues.

As hereinabove stated, the biologically active peptides or proteins of the present invention are preferably ion channel-forming peptides. An ion channel-forming peptide or protein or ionophore is a peptide or protein which increases the permeability for ions across a natural or synthetic lipid membrane. B. Christensen, et al., PNAS, Vol. 85, pgs. 5072-5076 (July 1988) describes methodology which indicates whether or not a peptide or protein has ion channel-forming properties and is therefore an ionophore. As used herein, an ion channel-forming peptide or ion channel-forming protein is a peptide or protein which has ion channel-forming properties as determined by the method of Christensen, et al.

An amphiphilic peptide or protein is a peptide or protein which includes both hydrophobic and hydrophilic peptide or protein regions.

The ion channel-forming peptides employed in the present invention are generally water soluble to a concentration of at least 20 mg/ml at neutral pH in water. In addition, the structure of such peptide provides for flexibility of the peptide molecule. Such peptides are capable of forming an alpha-helical structure. When the peptide is placed in water, it does not assume an amphiphilic structure. When the peptide encounters an oily surface or membrane, the peptide chain folds upon itself into a rodlike structure.

In general, such peptides have at least 7 amino acids, and in many cases have at least 20 amino acids. In most cases, such peptides do not have in excess of 40 amino acids.

The peptides and/or analogues or derivatives thereof may be administered to a host; for example a human or non-human animal, in an amount effective to inhibit growth of a target cell, virus, or virally-infected cell. Thus, for example, the peptides and/or analogues or derivatives thereof may be used as antimicrobial agents, anti-viral agents, anti-bacterial agents, anti-tumor agents, anti-parasitic agents, spermicides, as well as exhibiting other bioactive functions.

The term "antimicrobial" as used herein means that the polypeptides or proteins of the present invention inhibit, prevent, or destroy the growth or proliferation of microbes such as bacteria, fungi, viruses, or the like.

The term "anti-bacterial" as used herein means that the peptides or proteins employed in the present invention produce effects adverse to the normal biological fimctions of bacteria, including death or destruction and prevention of the growth or proliferation of the bacteria when contacted with the peptides or proteins. The term "antibiotic" as used herein means that the peptides or proteins employed in the present invention produce effects adverse to the normal biological functions of the non-host cell, tissue or organism, including death or destruction and prevention of the growth or proliferation of the non-host cell, tissue, or organism when contacted with the peptides or proteins.

The term "spermicidal" as used herein means that the peptides or proteins employed in the present invention, inhibit, prevent, or destroy the motility of sperm.

The term "anti-fungal" as used herein means that the peptides or proteins employed in the present invention inhibit, prevent, or destroy the growth or proliferation of fungi.

The term "antiviral" as used herein means that the peptides or proteins employed in the present invention inhibit, prevent, or destroy the growth or proliferation of viruses, or of virally-infected cells.

The term "anti-tumor" as used herein means that the peptides or proteins inhibits the growth of or destroys tumors, including cancerous tumors.

The term "anti-parasitic" as used herein means that the peptides or proteins employed in the present invention inhibit, prevent, or destroy the growth or proliferation of parasites.

The peptides or proteins of the present invention have a broad range of potent antibiotic activity against a plurality of microorganisms including gram-positive and gram-negative bacteria, fungi, protozoa, and the like, as well as parasites. The peptides or proteins of the present invention allow a method for treating or controlling microbial infection caused by organisms which are sensitive to the peptides or proteins. Such treatment may comprise administering to a host organism or tissue susceptible to or affiliated with a microbial infection an antimicrobial amount of at least one of the peptides or proteins .

Because of the antibiotic, antimicrobial, antiviral, and antibacterial properties of the peptides or proteins, they may also be used as preservatives or sterilants or disinfectants of materials susceptible to microbial or viral contamination.

The peptides or proteins and/or derivatives or analogues thereof may be administered in combination with a non-toxic pharmaceutical carrier or vehicle such as a filler, non-toxic buffer, or physiological saline solution. Such pharmaceutical compositions may be used topically or systemically and may be in any suitable form such as a liquid, solid, semi-solid, injectable solution, tablet, ointment, lotion, paste, capsule, or the like. The peptide or protein compositions may also be used in combination with adjuvants, protease inhibitors, or compatible drugs where such a combination is seen to be desirable or advantageous in controlling infection caused by harmful microorganisms including protozoa, viruses, and the like, as well as by parasites.

The peptides or proteins of the present invention may be administered to a host; in particular a human or non-human animal, in an effective antibiotic and/or anti-tumor and/or anti-fungal and/or anti-viral and/or anti-microbial and/or antibacterial and/or anti-parasitic and/or spermicidal amount.

Depending on the use, a composition in accordance with the invention will contain an effective anti-microbial amount and/or an effective spermicidal amount and/or an effective anti-fungal amount and/or an effective anti-viral amount and/or an effective anti-tumor amount and/or an effective anti-parasitic and/or an effective antibiotic amount of one or more of the peptides or proteins of the present invention which have such activity. The peptides or proteins may be administered by direct application of the peptides or proteins to the target cell or virus or virally-infected cell, or indirectly applied through systemic administration.

The peptides or proteins of the present invention may also be employed in promoting or stimulating healing of a wound in a host.

The term "wound healing" as used herein includes various aspects of the wound healing process. These aspects include, but are not limited to, increased contraction of the wound, increased deposition of connective tissue, as evidenced by, for example, increased deposition of collagen in the wound, and increased tensile strength of the wound, i.e., the peptides or proteins increase wound breaking strength. The peptides or proteins of the present invention may also be employed so as to reverse the inhibition of wound healing caused by conditions which depress or compromise the immune system.

The peptides or proteins of the present invention may be used in the treatment of external burns and to treat and/or prevent skin and burn infections. In particular, the peptides or proteins may be used to treat skin and burn infections caused by organisms such as, but not limited to, P. aeruginosa and S. aureus.

The peptides or proteins are also useful in the prevention or treatment of eye infections. Such infections may be caused by bacteria such as, but not limited to, P. aeruginosa, S . aureus. and N. gonorrhoeae. by fungi such as but not limited to C. albicans and A. fumigatus, by parasites such as but not limited to A. castellani, or by viruses.

The peptides or proteins may also be effective in killing cysts, spores, or trophozoites of infection - causing organisms. Such organisms include, but are not limited to Acanthamoeba which forms trophozoites or cysts, C. albicans, which forms spores, and A. fumigatus, which forms spores as well.

The peptides or proteins may also be administered to plants in an effective antimicrobial or antiviral or antiparasitic amount to prevent or treat microbial or viral or parasitic contamination thereof.

The peptides or proteins, when used in topical compositions, are generally present in an amount of at least 0.1%, by weight. In most cases, it is not necessary to employ the peptide in an amount greater than 2.0%, by weight.

In employing such compositions systemically (intramuscular, intravenous, intraperitoneal), the active peptide or protein is present in an amount to achieve a serum level of the peptide of at least about 5 ug/ml. In general, the serum level of peptide or protein need not exceed 500 ug/ml. A preferred serum level is about 100 ug/ml. Such serum levels may be achieved by incorporating the peptide or protein in a composition to be administered systemically at a dose of from 1 to about 10 mg/kg. In general, the peptide(s) or protein(s) need not be administered at a dose exceeding 100 mg/kg.

The peptides or proteins may be produced by known techniques and obtained in substantially pure form. For example, the peptides may be synthesized on an automatic peptide synthesizer. Journal of the American Chemical Society, Vol. 85, pgs. 2149-54 (1963). It is also possible to produce such peptides or proteins by genetic engineering techniques. The codons encoding specific amino acids are known to those skilled in the art, and therefore DNA encoding the peptides may be constructed by appropriate techniques, and one may clone such DNA into an appropriate expression vehicle (e.g., a plasraid) which is transfected into an appropriate organism for expression of the peptide or protein.

Upon production or synthesis of the peptide or protein, the N-terminal (NH- or amino terminal) of the peptide is reacted such that the lipophilic moiety is attached to the N-terminal of the peptide. For example, the reaction may be a condensation reaction with an amine. When the lipophilic moiety T is

the N-terminal is reacted with a carboxylic acid

of the formula R-COOH, wherein R is a hydrocarbon having at least 2 carbon atoms. The reaction may be carried out in the presence of a coupling agent, such as, for example, DCC, or DIC, and HOBT, or in the presence of an acid chloride. Such a reaction results in the formation of an N-terminal substituted peptide or protein having the structural formula hereinabove described.

In one embodiment, X is a peptide which is a basic (positively charged) polypeptide having at least sixteen amino acids wherein the polypeptide includes at least eight hydrophobic amino acids and at least eight hydrophilic amino acids. Still more particularly, the hydrophobic amino acids are in groups of two adjacent amino acids, and each group of two hydrophobic amino acids is spaced from another group of two hydrophobic amino acids by at least one amino acid other than a hydrophobic amino acid (preferably at least two amino acids) and generally by no greater than four amino acids, and the amino acids between pairs of hydrophobic amino acids may or may not be hydrophilic.

The hydrophilic amino acids are generally also in groups of two adjacent amino acids in which at least one of the two amino acids is a basic hydrophilic amino acid, with such groups of two hydrophilic amino acids being spaced from each other by at least one amino acid other than a hydrophilic amino acid (preferably at least two amino acids) and generally no greater than four amino acids, and the amino acids between pairs of hydrophilic amino acids may or may not be hydrophobic.

In accordance with a particularly preferred embodiment, the polypeptide comprises a chain of at least four groups of amino acids, with each group consisting of four amino acids. Two of the four amino acids in each group are hydrophobic amino acids, and two of the four amino acids in each group are hydrophilic, with at least one of the hydrophilic amino acids in each group being a basic hydrophilic amino acid and the other being a basic or neutral hydrophilic amino acid.

The hydrophobic amino acids may be selected from the class consisting of Ala, Cys, Phe, Gly, Ile, Leu, Met, Pro, Val, Trp, Tyr, norleucine (Nle), norvaline (Nva), and cyclohexylalanine (Cha). The neutral hydrophilic amino acids may be selected from the class consisting of Asn, Gln, Ser, Thr and homoserine (Hse). The basic hydrophilic amino acids may be selected from the class consisting of Lys, Arg, His, Orn, homoarginine (Har), 2, 4-diaminobutyric acid (Dbu), and p-aminophenylalanine.

Each of the groups of four amino acids may be of the sequence ABCD, BCDA, CDAB, or DABC, wherein A and B are each hydrophobic amino acids and may be the same or different, one of C or D is a basic hydrophilic amino acid, and the other of C or D is a basic or neutral hydrophilic amino acid and may be the same or different. In one embodiment, the polypeptide chain may comprise 5 or 6 groups of this sequence. In each group, each of A, B, C and D may be the same in some or all of the groups or may be different in some or all of the groups.

The polypeptide chain preferably has at least 20 amino acids, and no greater than 50 amino acids. It is to be understood, however, that the polypeptide does not have to consist entirely of the groups described above. The polypeptide may have amino acids extending from either or both ends of the noted groups forming the polypeptide chain and/or there may be amino acids between one or more of the at least four groups and still remain within the scope of the invention.

The groups of amino acids may be repeating groups of amino acids, or the amino acids in the various groups may vary provided that in each group of the at least four groups of amino acids there are two hydrophobic and two hydrophilic amino acids as hereinabove noted.

Thus the biologically active polypeptide may comprise a chain including at least four groups of amino acids, each containing four amino acids. Two of the four amino acids in each group are hydrophobic, at least one amino acid is basic hydrophilic, and the remaining one is basic or neutral hydrophilic, with the polypeptide chain preferably having at least 20 amino acids but no greater than 50 amino acids.

In one embodiment, each of the at least four groups of amino acids which are in the peptide chain is of the sequence A-B-C-D, B-C-D-A, C-D-A-B or D-A-B-C wherein A and B are hydrophobic amino acids, one of C or D is a basic hydrophilic amino acid, and the other of C or D is basic or neutral hydrophilic amino acid. The resulting polypeptide chain, therefore, may have one of the following sequences:

(X₁)_a(A-B-C-D)_n(Y₁)_b

(X₂)_a(B-C-D-A)_n(Y₂)_b

(X₃)_a(C-D-A-B)_n(Y₃)_b (X₄)_a(D-A-B-C)_n(Y₄)_b

wherein X₁ is D; C-D- or B-C-D-, Y is -A or -A-B or

-A-B-C

X₂ is A-, D-A- or C-D-A-

Y is -B, -B-C or B-C-D

X₃is B-, A-B-, D-A-B-

Y₃ is -C, -C-D, -C-D-A

X₄is C-, B-C-, A-B-C-

Y₄ is -D, -D-A, -D-A-B

a is 0 or 1; b is 0 or 1

and n is at least 4.

It is to be understood that the peptide chain may include amino acids between the hereinabove noted groups of four amino acids provided that the spacing between such groups and the charge on the amino acids does not change the characteristics of the peptide chain which provide amphiphilicity and a positive charge and do not adversely affect the folding characteristics of the chain to that which is significantly different from one in which the hereinabove noted groups of four amino acids are not spaced from each other.

As representative examples of such peptides, there may be mentioned.

I Ala-Phe-Ser-Lys-Ala-Phe-Ser-Lys-Ala-Phe-Ser- Lys-Ala-Phe-Ser-Lys-Ala-Phe-Ser-Lys

(SEQ ID NO: 1)

II Ala-Phe-Ser-Lys-Ala-Phe-Ser-Lys-Ala-Phe-Ser- Lys -Ala-Phe-Ser-Lys-Ala-Phe-Ser-Lys-Ala-Phe- Ser-Lys . (SEQ ID NO: 2)

III Phe-Ser-Lys-Ala-Phe-Ser- Lys-Ala-Phe-Ser-Lys-Ala- Phe-Ser-Lys-Ala- (SEQ ID NO: 3)

IV Ser-Lys-Ala-Phe-Ser-Lys-Ala- Phe-Ser-Lys-Ala-Phe-Ser-Lys-Ala- Phe-Ser-Lys-Ala-Phe- (SEQ ID NO: 4)

V Lys-Ala-Phe-Ser-Lys-Ala-Phe-Ser-Lys-Ala-Phe-Ser- Lys-Ala-Phe-Ser (SEQ ID NO: 5)

The peptide may have amino acids extending from either end of the chain. For example, the chains may have a Ser-Lys sequence before the "Ala" end, and/or an Ala-Phe sequence after the "Lys" end. Other amino acid sequences may also be attached to the "Ala" and/or the "Lys" end.

Similarly, in any polypeptide chain having at least four groups of amino acids of the sequence as described above, the chain may have, for example, a C-D sequence before the first A-B-C-D group. Also other amino acid sequences may be attached to the "A" and/or the "D" end of one of these polypeptide chains. Also there may be amino acids in the chain which space one or more groups of the hereinabove noted four amino acids from each other.

In accordance with another embodiment, X is a magainin peptide. A magainin peptide is either a magainin such as magainin I, II or III or an analogue or derivative thereof. The magainin peptides preferably include the following basic peptide structure X₁₂

-- R₁₁-R₁₁-R₁₂-R₁₃-R₁₁-R₁₄-R₁₂-R₁₁-

R₁₄-R₁₂-R₁₁-R₁₁-R₁₁-R_14a-(R₁₅)_n-R_14a-R₁₄ - - wherein R₁₁ is a hydrophobic amino acid, R₁₂ is a basic hydrophilic amino acid; R₁₃ is a hydrophobic, neutral hydrophilic, or basic hydrophilic amino acid; R₁₄ and R_14a are hydrophobic or basic hydrophilic amino acids; R₁₅ is glutamic acid or aspartic acid, or a hydrophobic or a basic hydrophilic amino acid, and n is 0 or 1. In a preferred embodiment, R₁₃ is a hydrophobic or neutral hydrophilic amino acid, R_14a is a hydrophobic amino acid, and R₁₅ is glutamic acid or aspartic acid.

Thus, for example, a magainin peptide may include the following structure:

-Y₁₂-X₁₂- where X₁₂ is the hereinabove described basic peptide structure and Y₁₂ is

(i) R₁₂

(ii) R_14a-R₁₂

(iii) R₁₁-R_14a-R₁₂

(iv) R₁₄-R₁₁-R_{14 a} - R_{1 2}

where R₁₁, R₁₂, R₁₄ and R_14a are as previously defined. A magainin peptide may also have the following structure:

-X₁₂-Z₁₂- wherein X₁₂ is as previously defined and Z₁₂ is:

(i) R₁₆ where R₁₆ is a basic hydrophilic amino acid or asparagine or glutamine.

(ii) R₁₆-R₁₇ where R₁₇ is a neutral hydrophilic amino acid, a hydrophobic amino acid, or a basic hydrophilic amino acid. Preferably, R₁₇ is a neutral hydrophilic amino acid.

A magainin peptide may also have the following structure: ( Y₁₂)_a-X₁₂-(Z₁₂)_b where X₁₂, Y₁₂and Z₁₂ are as previously defined and a is 0 or 1 and b is 0 or 1.

The magainin peptides may also include the following basic peptide structure X₁₃:

╌R₁₄-R₁₁-R_14a-R₁₂-R₁₁-R₁₁-R₁₂-R₁₃-

R₁₁-R₁₄-R₁₂-R₁₁-R₁₁-R₁₂-, wherein R₁₁,R₁₂,R₁₃, R₁₄, and

R_14a are amino acids as hereinabove described.

The magainin peptide may also include the following structure X₁₃-Z₁₃ ; wherein X₁₃ is the hereinabove described basic peptide structure and Z₁₃ is

(R₁₁)_n (R₁₁)_n-(R₁₁)_n-(R_14a)_n-(R ₁₅)_n-(R_14a)_n-(R _{14) n}╌ (R₁₆)_n-(R₁₇)_n wherein R₁₁, R₁₄, R_14a, R₁₅ , R₁₆, and R₁₇ are as hereinabove described, and n is 0 or 1, and each n may be the same or different.

The magainin peptides generally include at least fourteen amino acids and may include up to forty amino acids. A magainin peptide preferably has 22 or 23 amino acids. Accordingly, the hereinabove described basic peptide structures of a magainin peptide may include additional amino acids at the amino end or at the carboxyl end, or at both ends.

As representative examples of such magainin peptides, there may be mentioned peptides having the following primary sequences as given in the accompanying sequence listing as well as appropriate analogues and derivatives thereof:

(a) (SEQ ID NO:6) (OH) or (NH₂)

(Magainin I)

(b) (SEQ ID NO: 7) (OH) or (NH₂)

(Magainin II)

(c) (SEQ ID NO: 8) (OH) or (NH₂)

(Magainin III)

The following are examples of peptide derivatives or analogs of the basic structure:

(d) (SEQ ID NO: 9) (OH) or (NH₂)

(e) (SEQ ID NO: 10) (OH) or (NH₂)

(f) (SEQ ID NO: 11) (OH) or (NH₂)

Magainin peptides are described in Proc. Natl. Acad Sci. Vol. 84 pp. 5449-53 (Aug. 87). The term "magainin peptides" as used herein refers to the basic magainin structure as well as derivatives and analogs thereof, including but not limited to the representative derivatives or analogs.

In accordance with a further embodiment, X may be a PGLa peptide or an XPF peptide.

A PGLa peptide is either PGLa or an analogue or derivative thereof. The PGLa peptides preferably include the following basic peptide structure X₁₄:

- R₁₁-R₁₇-R₁₂-R₁₁-R₁₄-R₁₄-R₁₁-

R₁₁-R₁₄-R₁₂-R_{1 1}-R₁₁-R₁₂-R₁₁-

R₁₁-R₁₁-R₁₂- where R₁₁, R₁₂, R₁₄ and R₁₇ are as previously defined.

The PGLa peptides generally include at least seventeen amino acids and may include as many as forty amino acids. Accordingly, the hereinabove described basic peptide structure for a PGLa peptide may include additional amino acids at the amino end or at the carboxyl end or at both the amino and carboxyl end.

Thus, for example, a PGLa peptide may have the following structure:

-Y₁₄-X₁₄- where X₁₄ is as previously defined and

^Y14 ^is

(i) R₁₁;

(ii) ^R14^-R11 where R₁₁ and R₁₄ are as previous ly defined.

For example, a PGLa peptide may also have the following structure:

-X₁₄-Z₁₄- where X₁₄ is as previous ly defined; and Z₁₄ is :

( i) R _{1 1}; or

( ii) R _{1 1}-R_{1 1}

where R₁₁ is as previously defined.

A PGLa peptide may also have the following structure:

(Y₁₄)_a-X₁₄-(Z₁₄)_b where X₁₄; Y₁₄ and Z₁₄ are as previously defined, a is 0 or 1 and b is 0 or 1.

An XPF peptide is either XPF or an analogue or derivative thereof. The XPF peptides preferably include the following basic peptide structure X₁₆:

--R₁₁-R₁₇-R₁₂-R₁₁-R₁₄-R₁₈-R₁₇-

R₁₁-R₁₄-R₁₂-R₁₁-R₁₁-R₁₂-

R₁₁-R₁₁- R₁₁-R₁₂-(R₁₅ )_n-R₁₁╌ wherein

R₁₁- R₁₂- R₁₄- R₁₅ and R₁₇ are as previously defined and R₁₈ is glutamine or asparagine or a basic hydrophilic, or hydrophobic amino acid and, n is 0 or 1.

The XPF peptides generally include at least nineteen amino acids and may include up to forty amino acids.

Accordingly, the hereinabove described basic peptide structure of XPF may include additional amino acids at the amino end, or at the carboxyl end or at both the amino and carboxyl ends.

Thus, for example, an XPF peptide may include the following structure:

-Y₁₆-X₁₆- where X₁₆ is as previously defined and Y₁₆ is

(i) R₁₁ or

(ii) R₁₄-R₁₁

where R₁₁ and R₁₄ are as previously defined.

An XPF peptide may include the following structure:

-X₁₆- Z₁₆- where X₁₆ is as previously defined and Z₁₆ is

(i) R₁₁; or

(ii) R₁₁-R₁₈; or

(iii) R₁₁-R₁₈-Proline; or

(iv) R₁₁-R₁₈-Proline-R₁₂

An XPF peptide may also have the following structure:

(Y₁₆)_a-X₁₆-(Z₁₆)_b where X₁₆, Y₁₆ and Z₁₆ are as previously defined: a is 0 or 1 and b is 0 or 1.

Preferred are XPF or PGLa peptides, which are characterized by the following primary amino acid sequences as given in the accompanying sequence listing:

PGLa : (SEQ ID NO: 12) (NH₂)

XPF : (SEQ ID NO: 13) A review of XPF and PGLa can be found in Hoffman et al, EMBO J. 2:711-714,. 1983; Andreu, et al, J. Biochem. 149:531-535, 1985; Gibson, et al J. Biol. Chem. 261:5341-5349, 1986; and Giovannini, et al, Biochem J. 243:113-120, 1987.

In accordance with yet another embodiment, X is a CPF peptide or appropriate analogue or derviative thereof.

CPF peptides as well as analogues and derivatives thereof are herein sometimes referred to collectively as CPF peptides.

The CPF peptide may be one which includes the following basic peptide structure X-,-,:

-R₂₁-R₂₁-R₂₂-R₂₂-R₂₁-R₂₁-R₂₃-R₂₁-

-R₂₁-R₂₁-R₂₃-R₂₁-R₂₁-R₂₄-R₂₅-R₂₁- wherein R₂₁ is a hydrophobic amino acid;

R₂₂ is a hydrophobic amino acid or a basic hydrophilic amino acid;

R₂₃ is a basic hydrophilic amino acid;

R₂₄ is a hydrophobic or neutral hydrophilic amino acid; and

R₂₅ is a basic or neutral hydrophilic amino acid.

The hereinabove basic structure is hereinafter symbolically indicated as X₂₀.

The hydrophobic amino acids are Ala, Cys, Phe, Gly, Ile, Leu, Met, Val, Trp, Tyr, norleucine (Nle), norvaline (Nva), and cyclohexylalanine (Cha). The neutral hydrophilic amino acids are Asn, Gln, Ser, Thr, and homoserine (Hse).

The basic hydrophilic amino acids are Lys, Arg, His, Orn, homoarginine (Har), 2,4-diaminobutyric acid (Dbu), and p-aminophenylalanine.

The CPF peptide may include only the hereinabove noted amino acids or may include additional amino acids at the amino and/or carboxyl end or both the amino and carboxyl end. In general, the peptide does not include more than 40 amino acids.

The CPF peptides including the above basic structure preferably have from 1 to 4 additional amdLno acids at the amino end.

Accordingly, such preferred peptides may be represented by the structural formula:

Y₂₀ - X₂₀ - wherein X₂₀ is the hereinabove described basic peptide structure and Y₂₀ is

(i) R₂₅-, or

(ii) R₂₂-R₂₅-; or

(iii) R₂₁-R₂₂-R₂₅; or

(iv) R₂₂-R₂₁-R₂₂-R₂₅; PreferablY

Glycine - R₂₁-R₂₂-R₂₅.

wherein R₂₁, R₂₂ and R₂₅ are as previously defined.

The carboxyl end of the basic peptide structure may also have additional amino acids which may range from 1 to 13 additional amino acids. In a preferred embodiment, the basic structure may have from 1 to 7 additional amino acids at the carboxyl end, which may be represented as follows:

-X₂₀ - Z₂₀ wherein

X is the hereinabove defined basic peptide structure and Z₂₀ is

(i) R₂₁-, or

(ii) R₂₁-R₂₁-; or

(iii) R₂₁-R₂₁-R₂₄; or

( iv) R₂₁-R₂₁-R₂₄-R₂₄; or

(v) R₂₁-R₂₁-R₂₄-R₂₄-R₂₆; or

(vi) R₂₁-R₂₁-R₂₄-R₂₄-R₂₆-Gln; or

(vii) R₂₁-R₂₁-R₂₄-R₂₄-R₂₆-Gln-Gln, wherein R₂₁ and R₂₄ are as previously defined, and R₂₆ is proline or a hydrophobic amino acid.

Preferred peptides may be represented by the following structural formula

(Y₂₀)_a - X₂₀ - (Z₂₀)_b wherein X₂₀, Y₂₀ and Z₂₀ are as previously defined and a is 0 or 1 and b is 0 or 1.

Representative examples of CPF peptides which may be employed, some of which have been described in the literature, include the following sequences as given in the accompanying sequence listing:

(SEQ ID NO: 14)

(SEQ ID NO: 15) (SEQ ID NO: 16)

(SEQ ID NO: 17)

(SEQ ID NO: 18)

(SEQ ID NO: 19)

(SEQ ID NO: 20)

(SEQ ID NO: 21)

(SEQ ID NO: 22)

(SEQ ID NO: 23)

(SEQ ID NO: 24)

(SEQ ID NO: 25)

(SEQ ID NO: 26)

A review of the CPF peptides can be found in Richter, K.,

Egger, R., and Kreil (1986) J. Biol. Chem 261, 3676-3680;

Wakabayashi, T., Kato, H., and Tachibaba, S. (1985)

Nucleic Acids Research 13, 1817-1828; Gibson, B.W.,

Poulter, L., Williams, D.H., and Maggio, J.E. (1986) J.

Biol. Chem 261, 5341-5349.

In accordance with yet another embodiment, X is a peptide which includes one of the following basic structures X₃₁ through X₃₇ wherein: X₃₃ is -[R₃₁-R₃₂-R₃₂-R_{3 3}-R₃₁-R₃₂-R₃₂ ]-_n;

X₃₂ is -[R₃₂-R₃₂-R₃₃-R_{3 1}-R₃₂-R₃₂-R₃₁ ]-_n;

X₃₃ is -[R₃₂-R₃₃-R₃₁-R₃₂-R₃₂-R₃₁-R₃₂ ] -_n;

X₃₄ is -[R₃₃-R₃₁-R₃₂-R₃₂-R₃₁-R₃₂-R₃₂] -_n ;

X₃₅ is =[R₃₁-R₃₂-R₃₂-R₃₁-R₃₂-R₃₂-R₃₃] -_n ;

X₃₆ is -[R₃₂-R₃₂-R₃₁-R₃₂-R₃₂-R₃₃-R₃₂ ] -_n;

X₃₇ is -[R₃₂-R₃₁-R₃₂-R₃₂-R₃₃-R₃₁-R₃₂ ] -_n; wherein R₃₁ is a basic hydrophilic amino acid, R,-. is a hydrophobic amino acid, R₃₃ is a neutral hydrophilic, basic hydrophilic, or hydrophobic amino acid, and n is from 2 to 5.

The basic hydrophilic amino acids may be selected from the class consisting of Lys, Arg, His, Orn, homoarginine (Har), 2,4-diaminobutyric acid (Dbu), and p-aminophenylalanine.

The hydrophobic amino acids may be selected from the class consisting of Ala, Cys, Phe, Gly, Ile, Leu, Met, Pro, Val, Trp and Tyr,norleucine (Nle), norvaline (Nva), and cyclohexylalanine (Cha).

The neutral hydrophilic amino acids may be selected from the class consisting of Asn, Gln, Ser, Thr, and homoserine (Hse).

In accordance with one embodiment, when the peptide includes the structure X₃₁, the peptide may include the following structure:

X₃₁-X₃₁, wherein X₃₁ is as hereinabove described, and Y₃₁ is:

(i) R₃₂;

(ii) R₃₂-R₃₂;

(iii) R₃₁-R₃₂-R₃₂;

(iv) R₃₃-R₃₁-R₃₂-R₃₂;

(v) R₃₂-R₃₃-R₃₁-R₃₂-R₃₂; or

(vi) R₃₂-R₃₂-R₃₃-R₃₁-R₃₂-R₃₂, wherein R₃₁, R₃₂, and R₃₃ are as hereinabove described In accordance with another embodiment, when the peptide includes the structure X₃₁, the peptide may include the following structure:

X₃₁-Z₃₁, wherein X₃₁ is as hereinabove described, and Z₃₁ is:

(i) R₃₁;

(ii) R₃₁-R₃₂;

(iii) R₃₁-R₃₂-R₃₂;

(iv) R₃₁-R₃₂-R₃₂-R₃₃;

(v) R₃₁-R₃₂-R₃₂-R₃₃-R₃₁; or

(vi) R₃₁-R₃₂-R₃₂-R₃₃-R₃₁-R₃₂.

In accordance with yet another embodiment, the peptide may include the following structure:

(Y₃₁) _a-X_{3 1}-(Z₃₁ )_b wherein Y₃₁ and Z₃₁ a re as previously defined, a is 0 or 1, and b is 0 or 1.

When the peptide includes the structure X₃₂, the peptide may include the following structure:

Y₃₂ - X₃₃, wherein X₃₂ is as hereinabove described, and Y₃₂ is:

(i) R₃₁;

(ii) R₃₂-R₃₁;

(iii) R₃₂-R₃₂-R₃₁;

(iv) R₃₁-R₃₂-R₃₂-R₃₁;

(v) R₃₃-R₃₁-R₃₂-R₃₂-R₃₁; or

(vi) R₃₂-R₃₃-R₃₁-R₃₂-R₃₂-R₃₁- In another embodiment, when the peptide includes the structure X₃₂, the peptide may include the following structure:

X₃₂ - Z₃₂ , where in X₂₃ is as hereinabove

and Z₃₂ is

( i) R₃₂;

( ii) R₃₂-R₃₂ ;

( iii) R₃₂-R₃₂-R₃₃ ;

(iv) R₃₂-R₃₂-R₃₃-R₃₁;

(v) R₃₂-R₃₂-R₃₃-R₃₁-R₃₂; or

(vi) R₃₂-R₃₂-R₃₃-R_{3 1}-R₃₂-R₃₂.

In accordance with yet another embodiment, the peptide may include the following structure:

(Y₃₂)_a - X₃₂ - (Z₃₂)_b, wherein Y₃₂ and Z₃₂ are as previously defined, a is 0 or 1, and b is 0 or 1.

In accordance with another embodiment, when the peptide includes the structure X₃₃, the peptide may include the following structure:

Y₃₃ - X₃₃ wherein X₃₃ is as hereinabove described,

Y₃₃ is

( i) R₃₂;

(ii) R₃₁ -R₃₂;

(iii) R₃₂ -R₃₁-R₃₂;

(iv) R₃₂-R₃₂-R₃₁-R₃₂;

(v) R₃₁-R₃₂-R₃₂-R₃₁-R₃₂; or

(vi) R₃₃-R₃₁-R₃₂-R₃₂-R₃₁-R₃₂. wherein R₃₁ , R₃₂, and R₃₃ are as hereinabove described. In accordance with another embodiment, when the peptide includes the structure R₃₃, the peptide may include the following structure:

X₃₃ - Z₃₃wherein R₃₃is as hereinabove described, and Z₃₃ is:

( i) R₃₂;

( ii) R₃₂-R₃₃ ;

( iii) R₃₂-R₃₃-R₃₁ ;

(iv) R₃₂-R₃₂-R₃₁-R₃₂;

(v) R₃₂-R₃₂-R₃₃-R₃₁-R₃₂; or

(vi) R₃₂-R₃₃-R₃₁-R_{3 2}-R₃₂-R₃₁.

In accordance with yet another embodiment, the peptide may include the following structure:

(Y₃₃)_a - X₃₃ - (Z₃₃)_b, wherein Y₃₃ and Z₃₃ are as previously defined, a is 0 or 1, and b is 0 or 1.

In accordance with yet another embodiment, when the peptide includes the structure X₃₄, the peptide may include the following structure:

Y₃₄- X₃₄, wherein R₃₄is as hereinabove described, and Y₃₄is:

(i) R₃₂;

(ii) R₃₂-R₃₂;

(iii) R₃₁-R₃₂-R₃₂;

(iv) R₃₂-R₃₁-R₃₂-R₃₂;

(v) R₃₂-R₃₂-R₃₁-R₃₂-R_32; or (vi) R₃₁-R₃₂-R₃₂-R₃₁-R₃₂-R₃₂, wherein R₃₁, R₃₂ and R₃₃are as hereinabove described.

In accordance with another embodiment, when the peptide includes the structure X₃₄, the peptide may include the following structure:

X₃₄-Z₃₄, wherein X₃₄, is as hereinabove described, and Z₃₄is:

(i) R₃₃;

(ii) R₃₃-R₃₁;

(iii) R₃₃-R₃₁-R₃₂;

(lv) R₃₃-R₃₁-R₃₂-R₃₂;

(v) R₃₃-R₃₁-R₃₂-R₃₂-R₃₁; or

(vi) R₃₃-R₃₁-R₃₂-R₃₂-R₃₁-R₃₂.

In accordance with yet another embodiment, the peptide may Include the following structure:

(Y₃₄)_a- X₃₄- (Z₃₄)_b, wherein X₃₄ and Z₃₄ are as previously defined, a is 0 or 1, and b is 0 or 1.

In accordance with a further embodiment, when the peptide includes the structure X₃₅, the peptide may include the following structure:

Y₃₅-X₃₅, wherein X₃₅ is as hereinabove described, and Y₃₅ is:

( i) R₃₃;

(ii) R₃₂-R₃₃;

(iii) R₃₂-R₃₂-R₃₃;

(iv) R₃₁-R₃₂-R₃₂-R₃₃;

(v) R₃₂-R₃₁-R₃₂-R₃₂-R₃₃; or (vi) R₃₂-R₃₂-R₃₁-R₃₂-R₃₂-R₃₃, wherein R₃₁, R₃₂, and R₃₃are as hereinabove described.

In accordance with another embodiment, when the peptide includes the structure X₃₅, the peptide may include the following structure:

X₃₅ - Z₃₅wherein X₃₅ is as hereinabove described, and R₃₂is:

(i) R₃₁;

(ii) R₃₁-R₃₂;

(iii) R₃₁-R₃₂-R₃₂;

(iv) R₃₁-R₃₂-R₃₂-R₃₁;

(v) R₃₁-R₃₂-R₃₂-R₃₁-R₃₂; or

(vi) R₃₁-R₃₂-R₃₂-R₃₁-R₃₂-R₃₂-

In accordance with yet another embodiment, the peptide may include the following structure:

(Y«) - X₃₅ (^Z35)_t,_> wherein X₃_ and Z-. are as previously defined, a is 0 or 1, and b is 0 or 1.

In accordance with a further embodiment, when the peptide includes the structure X₃₆, the peptide may include the following structure:

Y₃₆ - X₃₆ wherein X₃₆ is as hereinabove described, and Y₃₆is:

(i) R₃₁;

(ii) R₃₃-R₃₁;

(ill) R₃₂-R₃₃-R₃₁;

(iv) R₃₂-R₃₂-R₃₃-R₃₁;

(v) R₃₁-R₃₂-R₃₂-R₃₃-R₃₁; or (vi) R₃₂-R₃₁-R₃₂-R₃₂-R₃₃-R₃₁- wherein R₃₁, R₃₂, and R₃₃ are as hereinabove described.

In accordance with another embodiment, when the peptide includes the structure X36, the peptide may include the following structure:

X₃₆-Z₃₆ wherein X36 is as hereinabove described, and Z₃₆ is:

(i) R₃₂;

(ii) R₃₂-R₃₂;

(iii) R₃₂-R₃₂-R₃₁;

(iv) R₃₂-R₃₂-R₃₁-R₃₂;

(v) R₃₂-R₃₂-R₃₁-R₃₂-R₃₂; or

(vi) R₃₂-R₃₂-R₃₁-R₃₂-R₃₂-R₃₃.

In accordance with yet another embodiment, the peptide may include the following structure:

(Y₃₆)_a- X₃₆ (Z₃₆)_b, Wherein Y₃₆ and Z₃₆ are as previously defined, a is 0 or 1, and b is 0 or 1.

In accordance with one embodiment, when the peptide includes the structure X₃₇, the peptide may includes the structure Y₃₇-X₃₇, wherein X₃₇ is as hereinabove described, and Y₃₇ is:

(i) R₃₂;

(ii) R₃₁-R₃₂,

(iii) R₃₃-R₃₁-R₃₂;

(iv) R₃₂-R₃₃-R₃₁-R₃₂;

(v) R₃₂-R₃₂-R₃₃-R₃₁-R₃₂; or (vi) R₃₁-R₃₂-R₃₂-R₃₃-R₃₁-R₃₂, whereln R₃₁, R₃₂, and R₃₃ are as hereinabove described.

In accordance with a further embodiment, when the peptide includes the structure X₃₇, the peptide may include the following structure:

X₃₇ - Z₃₇ wherein X₃₇ is as hereinabove described, and R₃₇ is:

(i) R₃₂;

(ii) R₃₂-R₃₁;

(iii) R₃₂-R₃₁-R₃₂;

(iv) R₃₂-R₃₁-R₃₂-R₃₂;

(v) R₃₂-R₃₁-R₃₂-R₃₂-R₃₃; or

(vi) R₃₂-R₃₁-R₃₂-R₃₂-R₃₃-R₃₁.

In accordance with yet another embodiment, the peptide may Include the following structure:

(Y₃₇)_a- X₃₇ (Z₃₇)_b, wherein Y₃₇ and Z₃₇ are as previously defined, a is 0 or 1, and b is 0 or 1.

In a preferred embodiment, n is 3, and most preferably the peptide is of one of the following structures as given in the accompanying sequence listing:

(Lys Ile Ala Gly Lys Ile Ala)₃ (SEQ ID NO: 27).

(Lys Ile Ala Lys Ile Ala Gly)₃ (SEQ ID NO: 28).

(Lys Ile Ala Gly Lys Ile Gly)_g (SEQ ID NO: 29).

(Lys Leu Ala Gly Lys Leu Ala)₃ (SEQ ID N0:30).

(Lys Phe Ala Gly Lys Phe Ala)₃ (SEQ ID NO: 31).

(Lys Ala Leu Ser Lys Ala Leu)₃ (SEQ ID NO: 32).

(Lys Leu Leu Lys Ala Leu Gly)₃ (SEQ ID NO: 33). (Lys Ala Ile Gly Lys Ala Ile)₃ (SEQ ID NO: 34).

(Gly Ile Ala Lys Ile Ala Lys)₃ (SEQ ID NO: 35).

(Lys Ile Ala Lys Ile Phe Gly)₃ (SEQ ID NO: 36).

(Gly Ile Ala Arg Ile Ala Lys)₃ (SEQ ID NO: 37).

(Lys Phe Ala Arg Ile Ala Gly)₃ (SEQ ID NO: 38).

(Gly Phe Ala Lys Ile Ala Lys)₃ (SEQ ID NO: 39).

(Lys Ile Ala Gly Orn Ile Ala)₃ (SEQ ID NO: 40).

(Lys Ile Ala Arg Ile Ala Gly)₃ (SEQ ID N0:41).

(Orn Ile Ala Gly Lys Ile Ala)₃ (SEQ ID NO: 42).

(Gly Ile Ala Arg Ile Phe Lys)₃ (SEQ ID NO: 43).

(Lys Nle Ala Gly Lys Nle Ala)₃ (SEQ ID NO: 44).

(Lys Nle Ala Gly Lys Ile Ala)₃ (SEQ ID NO: 45).

(Lys Ile Ala Gly Lys Nle Ala)₃ (SEQ ID NO: 46).

(Lys Nva Ala Gly Lys Nva Ala)₃ (SEQ ID NO: 47).

(Lys Nva Ala Gly Lys Ile Ala)₃ (SEQ ID NO: 48).

(Lys Leu Leu Ser Lys Leu Gly)₃ (SEQ ID NO: 49).

(Lys Leu Leu Ser Lys Phe Gly)₃ (SEQ ID NO: 50).

(Lys Ile Ala Gly Lys Nva Ala)₃ (SEQ ID NO: 51).

(His Ile Ala Gly His Ile Ala)₃ (SEQ ID NO: 52).

(Ala Gly Lys Ile Ala Lys Ile)₃ (SEQ ID NO: 53).

(Ile Ala Lys Ile Ala Gly Lys)₃ (SEQ ID NO: 54).

(Lys Ile Ala Gly Arg Ile Ala)₃ (SEQ ID NO: 55).

(Arg Ile Ala Gly Arg Ile Ala)₃ (SEQ ID NO:56).

(Lys Val Ala Gly Lys Ile Ala)₃ (SEQ ID NO:57).

(Lys Ile Ala Gly Lys Val Ala)₃ (SEQ ID NO: 58).

(Ala Lys Ile Ala Gly Lys Ile)₃ (SEQ ID NO: 59).

(Orn Ile Ala Gly Orn Ile Ala), (SEQ ID NO: 60). (Lys Phe Ala Gly Lys Ile Ala)₃ (SEQ ID NO: 61).

(Lys Ile Ala Gly Lys Phe Ala)₃ (SEQ ID NO: 62).

(Lys Cha Ala Gly Lys Ile Ala)₃ (SEQ ID NO: 63).

(Lys Nle Ala Lys Ile Ala Gly)₃ (SEQ ID NO: 64).

(Arg Ile Ala Gly Lys Ile Ala)₃ (SEQ ID NO: 65).

(Har Ile Ala Gly Har Ile Ala)₃ (SEQ ID NO: 66).

(Xaa Ile Ala Gly Lys Ile Ala)₃ (SEQ ID NO: 67).

(Lys Ile Ala Gly Xaa Ile Ala)₃ (SEQ ID NO: 68).

In (SEQ ID NO: 67) and (SEQ ID NO: 68), Xaa is p-aminophenylalanine.

In accordance with another embodiment, X is a peptide which Includes the following basic structure R₄₀:

R₃₁-R₃₂-R₃₂-R₃₃-R₃₄-R₃₂-R₃₂-R₃₁-R₃₂-R₃₂-R₃₂-R₃₄-R₃₂-R₃₂, wherein R₃₁, R₃₂, and R₃₃ are as hereinabove described, and R₃₄ is a basic hydrophilic or hydrophobic amino acid.

In accordance with one embodiment, the peptide may include the following structure:

Y₄₀-X₄₀, wherein X₄₀ is as hereinabove described, and Y₄₀ is:

(i) R₃₂;

(ii) R₃₂-R₃₂;

(iii) R₃₄-R₃₂-R₃₂;

(iv) R₃₃-R₃₄-R₃₂-R₃₂;

(v) R₃₂-R₃₃-R₃₄-R₃₂-R₃₂;

(vi) R₃₂-R₃₂-R₃₃-R₃₄-R₃₂-R₃₂- or (vii) R₃₁-R₃₂-R₃₂-R₃₃-R₃₄-R₃₂-R₃₂,wherein R₃₁, R₃₂,

33 and 34 are as hereinabove described.

In accordance with another embodiment, X is a peptide which includes the following structure:

X₄₀-Z₄₀, wherein X₄₀ is as hereinabove described and

^Z40 ^is:

(i) R₃₁;

(ii) R₃₁-R₃₂;

(iii) R₃₁-R₃₂-R₃₂;

(iv) R₃₁-R₃₂-R₃₂-R₃₃;

(v) R₃₁-R₃₂-R₃₂-R₃₃-R₃₄;

(vi) R₃₁-R₃₂-R₃₂-R₃₃-R₃₄-R₃₂; or

(vii) R₃₁-R₃₂-R₃₂-R₃₃-R₃₄-R₃₂-R₃₂, Wherein R₃₁, R₃₂,

R₃₃, and R₃₄ are as hereinabove described.

In accordance with yet another embodiment the peptide may Include the following structure:

(Y₄₀)_a-X₄₀-(Z₄₀)_b, wherein Y₄₀ and Z₄₀ are as previously defined, a is 0 or 1, and b is 0 or 1. In a preferred embodiment, the peptide has the following structural formula as given in the accompanying sequence listing:

(SEQ ID NO: 69)

In another preferred embodiment, the peptide has the following structural formula as given in the accompanying sequence listing:

(SEQ ID NO: 70) In accordance with a further embodiment, the peptide has one of the one of the following structural formulae as given in the accompanying sequence listing:

(SEQ ID NO: 71)

(SEQ ID NO: 72)

(SEQ ID NO: 73)

(SEQ ID NO: 74)

(SEQ ID NO: 75)

(SEQ ID NO: 76)

(SEQ ID NO: 77)

(SEQ ID NO: 78)

(SEQ ID NO: 79)

(SEQ ID NO: 80)

(SEQ ID NO: 81)

(SEQ ID NO: 82)

(SEQ ID NO: 83)

(SEQ ID NO: 84)

(SEQ ID NO: 85)

In accordance with another embodiment, X is a peptide which Includes one of the following structural formulae:

(i) - (Lys Ile Ala Lys Lys Ile Ala)-_n,

(ii) - (Lys Phe Ala Lys Lys Phe Ala) _n-, and (iii) - (Lys Phe Ala Lys Lys Ile Ala) _n-, wherein n is from 2 to 5. Preferably, n is 3, and the peptide has one of the following structural formulae: (Lys Ile Ala Lys Lys Ile Ala) ₃

(SEQ ID NO: 86)

(Lys Phe Ala Lys Lys Phe Ala) ₃

(SEQ ID NO: 87)

(Lys Phe Ala Lys Lys Ile Ala) ₃

(SEQ ID NO: 88)

In accordance with another embodiment, the X is a peptide which is selected from the group consisting of the following structural formulae as given in the accompanying sequence listing:

(SEQ ID NO: 89)

(SEQ ID NO: 90)

(SEQ ID NO: 91)

(SEQ ID NO: 92)

In accordance with yet another embodiment, X is a cecropin or sarcotoxin.

The term cecroplns Includes the basic structure as well as analogues and derivatives thereof. The cecropins and analogues and derivatives thereof are described in Ann. Rev. Microbiol. 1987, Vol. 41, pages 103-26, in particular page 108, and In Christensen, et al., PNAS Vol. 85, pgs. 5072-76, which are hereby incorporated by reference.

The term sarcotoxins includes the basic materials as well as analogues and derivatives thereof. The sarcotoxins and analogues and derivatives thereof are described in Molecular Entomology, pages 369-78, in particular page 375, Alan R. Liss, Inc. (1987), which is hereby incorporated by reference.

In accordance with another embodiment, X is melittin or an analogue or derivative thereof.

Melittin is an amphipathic peptide consisting of 26 amino acid residues, and is isolated from honeybee (Apis mellifera) venom. Habermann, et al., Hoppe-Seyler's Zeitschrift Physiol. Chem.. Vol. 348, pgs. 37-50 (1987). Melittin has the following structural formula as represented by the three-letter amino acid code:

Gly Ile Gly Ala Val Leu Lys Val Leu

5

Thr Thr Gly Leu Pro Ala Leu Ile Ser

10 15

Trp Ile Lys Arg Lys Arg Gln Gln

20 25

(SEQ ID NO: 93)

In another embodiment, X is a amphiphilic peptide which includes the following basic structure X₅₀:

R₄₁-R₄₂-R₄₂-R₄₁-R₄₂-R₄₂-R₄₁-R₄₁-R₄₂-R₄₁-R₄₁.

R₄₁ is a hydrophobic amino acid, and R₄₂ is a basic hydrophilic or neutral hydrophilic amino acid.

In one embodiment, the peptide includes the basic structure Y₅₀-X₅₀ wherein X₅₀ is as hereinabove described and Y₅₀ is:

( i) R₄₁;

(ii) R₄₂-R₄₁; or (iii) R₄₂-R₄₂-R₄₁, wherein R₄₁ and R₄₂ are as hereinabove described.

In one embodiment, R₄₁ is leucine. In another embodiment, R₄₂ is lysine. Representative examples of peptides in accordance with this aspect of the present invention include those having the following structures:

(SEQ ID NO: 94)

(SEQ ID NO: 95)

(SEQ ID NO: 96)

(SEQ ID NO: 97)

In accordance with another embodiment, X is an amphiphilic peptide which includes the following basic structure X₅₂ :

R₄₂-R₄₁-R₄₂-R₄₂-R₄₁-R₄₁-R₄₂-R₄₂-R₄₁-R₄₂-R₄₂, wherein R₄₁ is a hydrophobic amino acid and R₄₂ is a basic hydrophilic or neutral hydrophilic amino acid.

In one embodiment R₄₁, is leucine. In another embodiment, R₄₂ is lysine.

In one embodiment, the peptide includes the basic structure Y₅₂-X₅₂, wherein X₅₂ is as hereinabove described, and Y₅₂ is:

(i) R₄₂;

(ii) ^R41^-R42^;

(iii) R₄₁-R₄₁-R₄₂;

(iv) R₄₂-R₄₁-R₄₁-R₄₂; or

(v) R₄₂-R₄₂-R₄₁-R₄₁-R₄₂. In one embodiment, the peptide may have the following structure;

Lys Lys Leu Leu Lys Lys Leu Lys Lys Leu

5 10

Leu Lys Lys Leu Arg Arg

15

(SEQ ID NO.: 98)

In another embodiment, the peptide includes the basic structure X₅₂ - Z₅₂, wherein X₅₂ is as hereinabove described, and Z₅₂ is:

( i) R₄₁;

(ii) R₄₁-R₄₁;

(iii) R₄₁-R₄₁-R₄₂;

(iv) R₄₁-R₄₁-R₄₂-R₄₂; or

(v) R₄₁-R₄₁-R₄₂-R₄₂-R₄₁;

In one embodiment, the peptide may have the following structure:

Lys Leu Lys Lys Leu Leu Lys Lys Leu Lys Lys Leu Leu Lys Lys Leu 5 10

15

(SEQ ID NO: 99)

In another embodiment, the peptide may include the structure: ( Y₅₂ ) _a - X₅₂ - (Z₅₂ )_b, Wherein X₅₂ , Y₅₂ and Z₅₂ are as hereinabove described, and a is 0 or 1, and b is 0 or

1.

In another embodiment X is a biologically active amphiphilic peptide which includes the following basic structure X₅₄:

R₄₁-R₄₂-R₄₂ -R₄₁-R₄₁ -R₄₂-R₄₂-R₄₁-R₄₂-R₄₂-R₄₁-R₄₁-R₄₂ -R₄₂- R₄₃-,

wherein R₄₁ and R₄₂ are as hereinabove described, and R₄₃ is a neutral hydrophilic amino acid.

In one embodiment, the peptide may have the following structure:

(SEQ ID NO: 100)

In another embodiment, the peptide may have the following structure:

(SEQ ID NO: 101)

In another embodiment, X is a biologically active amphiphilic peptide which includes the following basic structure X₅₆:

R₄₁-R₄₂-R₄₁-R₄₁-R₄₂-R₄₂-R₄₁-R₄₁-R₄₂-R₄₂-R₄₄, wherein R₄₁ and R₄₂ are as hereinabove described, and R₄₄ is a neutral hydrophilic amino acid or proline.

In one embodiment, the peptide may include the structure:

X₅₆-Z ₅₆, wherein X₅₆ is as hereinabove described, and Z₅₆ is:

(i) -R₄₂; ( ii) -R₄₂-R₄₂;

(iii) -R₄₂-R₄₂-R₄₁ ;

(iv) -R₄₂-R₄₂-R₄₁-R₄₁ ;

(v) -R₄₂-R₄₂-R₄₁-R₄₁-R₄₂;

(vi) -R₄₂-R₄₂-R₄₁-R₄₁-R₄₂-R₄₂; or

(vii) R₄₂-R₄₂-R₄₁-R₄₁-R₄₂-R₄₂-R₄₁.

In a preferred embodiment, the peptide may have one of the following structures:

(SEQ ID NO: 102); or

(SEQ ID NO: 103).

In another embodiment, X is a biologically active amphiphilic peptide which includes the following basic structure X₅₈:

R₄₁-R₄₁-R₄₂-R₄₂-R₄₁-R₄₂-R₄₂-R₄₁-R₄₁-R₄₂-R₄₂-R₄₁-R₄₃, wherein R₄₁, R₄₂, and R₄₃ are as hereinabove described.

In one embodiment, the peptide Includes the structure X₅₈-Z₅₈, wherein X₅₈ is as hereinabove described, and Z₅₈is:

( i) -R₄₁;

(ii) -R₄₁-R₄₅;

(ill) -R₄₁-R₄₅-R₄₅;

(iv) -R₄₁-R₄₅-R₄₅-R₄₃;

(v) -R₄₁-R₄₅-R₄₅-R₄₃-R₄₁;

(vi) -R₄₁-R₄₅-R₄₅-R₄₃-R₄₁-R₄₃;

(vii) -R₄₁-R₄₅-R₄₅-R₄₃-R₄₁-R₄₃-R₄₃;

(viii) -R₄₁-R₄₅-R₄₅-R₄₃-R₄₁-R₄₃-R₄₃-R₄₅; or ( ix) -R₄₁-R₄₅-R₄₅-R₄₃-R₄₁-R₄₃-R₄₃-R₄₅-R₄₃ , wherein R₄₁ and R₄₃ are as hereinabove described, and R₄₅ is proline.

In one embodiment, the peptide has the following structure:

(SEQ ID NO: 104).

In another embodiment, X is a biologically active amphiphilic peptide which includes the following basic structure X₆₀:

R₄₁-R₄₁-R₄₃-R₄₂-R₄₁-R₄₁-R₄₁-R₄₁-R₄₁-R₄₁-R₄₂-R₄₁-R₄₁-R₄₂-

R₄₂-R₄₁-R₄₁-R₄₂-R₄₂-R₄₁-, wherein R₄₁, R₄₂, and R₄₃ are as hereinabove described. In one embodiment, the peptide may have the following structure:

(SEQ ID NO: 105).

In accordance with another embodiment, X is a peptide which Includes the following basic structure X₆₂:

- R₄₁-R₄₂-R₄₂-R₄₁-R₄₂-R₄₂-R₄₁ - wherein R₄₁ and R₄₂ are as hereinabove described.

In one embodiment the peptide includes the following structure Y₆₂ - X₆₂, where X₆₂ is as hereinabove described, and Y₆₂ is:

(i) R₄₁;

(ii) R₄₂-R₄₂;

( iii) R₄₂-R₄₂-R₄₁ ; or

( iv) R₄₁-R₄₂-R₄₂-R₄₁. Representative examples of such peptides include the following, the sequences of which are given in the accompanying sequence listing:

(SEQ ID NO : 106)

( SEQ ID NO: 107 )

(SEQ ID NO : 108)

( SEQ ID NO : 109 )

(SEQ ID NO : 110)

( SEQ ID NO : 111 )

In one embodiment, the peptide includes the structure X₆₂-Z₆₂ , wherein X₆₂ is as hereinabove descibed, and Z₆₂ is:

(i) R₄₁

(ii) R₄₁-R₄₂;

(iii) R₄₁-R₄₂-R₄₂; or (iv) R₄₁-R₄₂-R₄₂-R₄₁- where R₄₁ and R₄₂ are as hereinabove described.

A representative example includes the following peptide having the structural formula given below and listed in the accompanying sequence listing:

(SEQ ID NO: 112)

In another embodiment, the peptide has the structure

(Y₆₂)_a- X₆₂- (Z₆₂)_b, wherein X₆₂, Y₆₂ and Z₆₂ are as hereinabove described, a is 0 or 1, and b is 0 or 1.

Representative examples of such peptides include the following, the structures of which are given in the accompanying sequence listing: ( SEQ ID NO : 113)

( SEQ ID NO : 114)

( SEQ ID NO : 115 )

(SEQ ID NO: 116)

In another embodiment, X is a peptide having the following structural formula:

(SEQ ID NO: 117)

In accordance with yet another embodiment, X is an ion channel-forming peptide or protein.

Ion channel-forming proteins or peptides which may be employed include defensins, also known as human neutrophil antimicrobial peptides (HNP), major basic protein (MBP) of eosinophils, bactericidal permeability-increasing protein (BPI), and a pore-forming cytotoxin called variously perforin, cytolysin, or pore-forming protein. Defensins are described in Selsted, et al., J. Clin. Invest., Vol. 76, pgs. 1436-1439 (1985). MBP proteins are described in Wasmoen, et al., J. Biol. Chem., Vol. 263, pgs 12559-12563. (1988). BPI proteins are described in Ooi, et al, J. Biol. Chem., Vol. 262, pgs. 14891-14894 (1987). Perforin is described in Henkart, et al., J. Exp. Med., 160: 75 (1984), and in Podack, et al., J. Exp. Med., 160:695 (1984). The above articles are hereby incorporated by reference. The term ion channel-forming proteins includes the basic structures of the ion channel-forming proteins as well as analogues and derivatives.

In accordance with yet another embodiment, each of the amino acid residues of the peptides or proteins may be a D-amino acid or glycine. Although the scope of this particular embodiment is not to be limited to any theoretical reasoning, it is believed that the above-mentioned peptides or proteins, when consisting entirely of D-amino acid or glycine residues, may have increased resistance to proteolytic enzymes while retaining their activity. Such peptides thus may be administered orally. Also, in accordance with another embodiment, all of the amino acid residues may be D-amino acid or glycine residues, or L-amino acid or glycine residues.

It is also to be understood that the peptides or proteins may be administered in combination with one another.

In accordance with another embodiment, the N-terminal substituted peptides or proteins of the present invention may be employed in combination with an ion having phamacological properties for the purposes hereinabove described.

An ion having pharmacological properties is one which when introduced into a target cell or virus or virally-infected cell inhibits and/or prevents and/or destroys the growth of the target cell, virus, or virally-infected cell.

Such an ion having pharmacological properties is one which in the absence of an ion channel forming peptide is unable to cross a natural or synthetic lipid membrane; in particular a cell or virus membrane, in sufficient amounts to affect a cell or virus adversely.

The peptide or protein and ion having pharmacological properties may be administered as a single composition or in separate compositions, and the single or separate compositions may include additional materials, actives and/or inactives, in addition to the peptide or protein and ion having pharmacological properties. As representative examples of ions having pharmacological properties which may be employed, there may be mentioned fluoride, peroxide, bicarbonate, silver, zinc, mercury, arsenic, copper, platinum, antimony, gold, thallium, nickel, selenium, bismuth, and cadmium ions.

The peptide or protein and the ion having pharmacological properties, whether administered or prepared in a single composition or in separate compositions, are employed in amounts effective to inhibit and/or prevent and/or destroy the growth of the target cell, virus, or virally-infected cell. In effect, the ion potentiates the action of the peptide, i.e., the amount of ion is effective to reduce the maximum effective concentration of the peptide or protein for inhibiting growth of a target cell, virus, or virally-infected cell.

The ion having pharmacological properties, when used topically, is generally employed in a concentration of from 0.05% to 2.0%. When used systemically, the ion is generally employed in an amount of from 1 to 10 mg. per kg. of host weight. Peptide or protein dosages may be within the ranges hereinabove described.

It is also to be understood that the peptide or protein and ion having pharmacological properties, may be delivered or administered in different forms; for example, the ion may be administered orally, while the peptide may be administered by IV or IP.

As representative examples of administering the peptide or protein and ion for topical or local administration, the peptide could be administered in an amount of up to about 1% weight to weight and the ion delivered in an amount of about 50mM (about 0.1%). Alternatively, the ion, in the form of a salt such as sodium fluoride, could be administered orally in conjunction with systemic administration of the peptide or protein. For example, the peptide or protein may be administered IV or IP to achieve a serum dose of 100 micrograms per milliliter (10 milligrams per kilogram) in conjunction with an oral dose of ion, in particular, sodium fluoride, of 10 meq per kilogram. In accordance with another embodiment, the peptides or proteins of the present invention may be administered to a host in combination with an antibiotic selected from the class consisting of bacitracins, gramacidin, polymyxin, vancomycin, teichoplanin, aminoglycosides, hydrophobic antibiotics, penicillins, raonobactams, or derivatives or analogues thereof.

The bacitracins, gramacidin, polymyxin, vancomycin, teichoplanin, and derivatives and analogues thereof, are a group of polypeptide antibiotics. A preferred bacitracin is bacitracin A.

Aminoglycoside antibiotics include tobramycin, kanamycin, amikacin, the gentamiclns (e.g., gentamicin C₁, gentamicin C₂, gentamicin C_1a), netilmicin, and derivatives and analogues thereof. The preferred aminoglycosides are tobramycin and the gentamiclns. The aminoglycosides, and the bacitracins hereinabove described, tend to be hydrophilic and water-soluble.

Penicillins which may be employed include, but are not limited to benzyl penicillin, ampicillin, methicillin (dimethoxyphenyl penicillin), tlcaricillin, penicillin V (phenoxymethyl penicillin), oxacillin, cloxacillin, dicloxacillin, flucloxacillln, amoxicillin, and amidinocillin. Preferred penicillins which may be employed are benzyl penicillin and ampicillin. A preferred monobactam which may be employed is aztreonam. As representative examples of hydrophobic antibiotics which may be used in the present invention, there may be mentioned macrolides such as erythromycin, rσxythromycin, clarithromycin, etc.; 9-N-alkyl derivatives of erythromycin; midecamycin acetate; azithromycin; flurithromycin; rifabutin; rokitamycin; a 6-O-methyl erythromycin A known as TE-031 (Taisho); rifapentine; benzypiperazinyl rifamycins such as CGP-7040, CGP-5909, CGP-279353 (Ciba-Geigy); an erythromycin A derivative with a cyclic carbamate fused to the C₁₁/C₁₂ position of a macrolide ring known as A-62514 (Abbott); AC-7230 (Toyo Jozo); benzoxazinorifamycin; difficidin; dirithromycin; a 3-N-piperdinomethylzaino methyl rifamycin SV known as FCE-22250 (Faπnitalia); M-119-a (Kirln Brewery); a 6-O-methyl-1-4"-O-carbamoyl erythromycin known as A-63075 (Abbott); 3-formylrifamycin SV-hydrazones with diazabicycloalkyl side chains such as CGP-27557 and CGP-2986 (Ciba-Geigy); and 16-membered macrolides having a 3-O-alpha-L-cladinosyl moiety, such as 3-O-alpha-L-cladinosyldeepoxy rosaramicin; tylosins and acyl demycinosyl tylosins.

In addition to the macrolides hereinabove described, rifamycin, carbenicillin, and nafcillin may be employed as well.

Other antibiotics which may be used (whether or not hydrophobic) are antibiotics which are 50-S ribosome inhibitors such as lincomycin; clindamycin; and chloramphenicol; etc.; antibiotics which have a large lipid like lactone ring, such as mystatin; pimaricin, etc.

The peptide or protein and antibiotic may be adminstered by direct administration to a target cell or by systemic or topical administration to a host which includes the target cell, in order to prevent, destroy or inhibit the growth of a target cell. Target cells whose growth may be prevented, inhibited, or destroyed by the administration of the peptides and antibiotic include Gram-positive and Gram-negative bacteria as well as fungal cells.

The antibiotic, such as those hereinabove described, or derivatives or analogues thereof, when used topically, is generally employed in a concetration of about 0.1% to about 10%. When used systemically, the antibiotic or derivative or analogue thereof is generally employed in an amount of from 1.25 mg. to about 45 mg. per kg. of host weight per day. Peptide or protein dosages may be those as hereinabove described.

As representative examples of administering the peptide or protein and antibiotic for topical or local administration, the peptide or protein could be administered in an amount of from about 0.1% to about 10% weight to weight, and the antibiotic is delivered in an amount of from about 0.1% to about 10% weight to weight. In accordance with another embodiment, the peptides or proteins of the present invention may be administered in combination with an antiparasitic agent or an antifungal agent.

Antiparasitic agents which may be employed include, but are not limited to, anti-protozoan agents. Examples of specific anti-parasitic agents which may be employed include, but are not limited to, pentamidine isethionate, and propamidine isethionate (Brolene).

Anti-fungal agents which may be employed include, but are not limited to, ketoconazole. It is also to be understood that certain anti-parasitic agents, may also have anti-fungal activity, and that certain anti-fungal agents may have anti-parasitic activity.

In accordance with another embodiment, the peptides or proteins of the present invention may be administered in combination with an antibiotic which inhibits DNA gyrase, which is an enzyme involved in the formation of bonds between individual coiling strands of replicating bacterial DNA. Thus, DNA gyrase is necessary for the normal replication of bacterial DNA, and, therefore, antibiotics which inhibit DNA gyrase inhibit the normal replication of bacterial DNA.

Examples of antibiotics which inhibit DNA gyrase include nalidixic acid, oxolinic acid, cinoxacin, and quinolone antibiotics which include ciprofloxacin, norfloxacin, ofloxacin, enoxacin, pefloxacin, lomefloxacin, fleroxacin, tosulfloxacin, temafloxacin, and rufloxacin.

The present invention will be further described with respect to the following example; however, the scope of the invention is not to be limited thereby.

EXAMPLE

Table I, which follows, indicates the Minimal Inhibitory Concentration (MIC) in μg/ml of Peptides (SEQ ID NO: 27), (SEQ ID NO: 110), (SEQ ID NO: 113), and (SEQ ID NO: 118), against S. aureus strain ATCC 25923, P. aeruginosa strain ATCC 27853, and E.coli ATCC strain 25922. The peptides are unsubstituted at the N-terminal, substituted with an acetyl group at the N-terminal as indicated by Ac-, or substituted with an octanoyl group at the N-terminal as indicated by Oct-.

The procedure for the antibacterial assay is based upon the guidelines of the National Committee for Clinical Laboratory Standards, Document M7-T2, Volume 8, No. 8, 1988.

Stock solutions of peptides (SEQ ID NO:27), (SEQ ID NO: 110), (SEQ ID NO: 113), and (SEQ ID NO: 118), with and without the appropriate substitutions, are prepared at a concentration of 512 μg/ml in sterile deionized distilled water and stored at -70°C. Each peptide is a C-terminal amide.

The stock peptide solution is diluted in serial dilutions (1:2) down the wells of a microtiter plate so that the final concentrations of peptides in the wells are 0.25, 0.50, 1, 2, 4, 8, 16, 32, 64, 128, and 256 μg/ml. 1-5 X 10⁵ CFUs/ml of either S. aureus ATCC 25923, E. coli ATCC 25922, or P. aeruginosa ATCC 27853 were added to the wells in full strength Mueller Hinton broth (BBL 11443) from a mid-log culture. The inoculum is standardized spectrophotometrically at 600 nm and is verified by colony counts. The plates are incubated for 16-20 hours at 37°C, and the minimal inhibitory concentration (MIC) for each peptide is determined. Minimal inhibitory concentration is defined as the lowest concentration of peptide which produces a clear well in the microtiter plate. The minimal inhibitory concentration of each of (SEQ ID NO: 27), (SEQ ID NO: 110), (SEQ ID NO: 113), and (SEQ ID NO: 118) with and without the appropriate substitutions is given in Table I below.

Table I

Minimal Inhibitory Concentration

( μg/ml )

Peptide S. aureus P. aeruginosa E. coli

(SEQ ID NO: 27)-NH₂ 8,16 64,128 8

Ac-(SEQ ID NO: 27)-NH₂ 32 128 8

Oct-(SEQ ID NO: 27)-NH₂ 2 4 2,4

(SEQ ID NO: 110)-NH₂ >256 32,64 64,128

Ac-(SEQ ID NO: 110)-NH₂ 256 8,16 32,64

Oct-(SEQ ID NO: 110)-NH₂ 8 4 16

(SEQ ID NO: 113)-NH₂ 16,32 8,16 32

Ac-(SEQ ID NO: 113)-NH₂ 32 64 64

Oct-(SEQ ID NO: 113)-NH₂ 8 4 16,32

(SEQ ID NO: 118)-NH₂ >256 256 256

Ac-(SEQ ID NO: 118)-NH₂ 256 256 256

Oct-(SEQ ID NO: 118)-NH₂ 8 8 32

The above results indicate that when a biologically active peptide is substituted with a lipophilic moiety of the present invention, the peptid has increased biological activity against a variety of microorganisms.

It is to be understood, however, that the scope of the present invention is not to be limited to the specific embodiments

described above. The invention may be practiced other than as

particularly described and still be within the scope of the

accompanying claims.

SEQUENCE LISTING

(1) GENERAL INFORMATION:

(i) APPLICANT: Karl, U. Prasad

(ii) TITLE OF INVENTION: Biologically Active Peptides Having N-Terminal Substitutions

(iii) NUMBER OF SEQUENCES: 118

(iv) CORRESPONDENCE ADDRESS:

(A) ADDRESSEE: Carella, Byrne, Bain,

Gilfillan, Cecchi & Stewart

(B) STREET: 6 Becker Farm Road

(C) CITY: Roseland

(D) STATE: New Jersey

(E) COUNTRY: USA

(F) ZIP: 07068

(v) COMPUTER READABLE FORM:

(A) MEDIUM TYPE: 3.5 inch diskette

(B) COMPUTER: IBM PS/2

(C) OPERATING SYSTEM: PC-DOS

(D) SOFTWARE: DW4.V2

(vi) CURRENT APPLICATION DATA:

(A) APPLICATION NUMBER:

(B) FILING DATE:

(C) CLASSIFICATION:

(vii) PRIOR APPLICATION DATA:

(A) APPLICATION NUMBER:

(B) FILING DATE:

(viii) ATTORNEY/AGENT INFORMATION:

(A) NAME: Olsteln, Elliot M.

(B) REGISTRATION NUMBER: 24,025

(C) REFERENCE/DOCKET NUMBER: 421250

(ix) TELECOMMUNICATION INFORMATION:

(A) TELEPHONE: 201-994-1700

(B) TELEFAX: 201-994-1744

(2) INFORMATION FOR SEQ ID NO:1:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 20 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(x) PUBLICATION INFORMATION: (H) DOCUMENT NUMBER: W089/11290

(I) FILING DATE: 19-MAY-1989

(J) PUBLICATION DATE: 30-NOV-1989

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:1:

Ala Phe Ser Lys Ala Phe Ser Lys Ala Phe

5 10

Ser Lys Ala Phe Ser Lys Ala Phe Ser Lys

15 20

(2) INFORMATION FOR SEQ ID NO: 2:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 24 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS :

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(x) PUBLICATION INFORMATION:

(H) DOCUMENT NUMBER: W089/11290

(I) FILING DATE: 19-MAY-1989

(J) PUBLICATION DATE: 30-N0V-1989

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 2: Ala Phe Ser Lys Ala Phe Ser Lys Ala Phe

5 10

Ser Lys Ala Phe Ser Lys Ala Phe Ser Lys

15 20

Ala Phe Ser Lys

(2) INFORMATION FOR SEQ ID NO: 3:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 16 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(x) PUBLICATION INFORMATION:

(H) DOCUMENT NUMBER: W089/11290

(I) FILING DATE: 19-MAY-1989

(J) PUBLICATION DATE: 30-NOV-1989

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 3: Phe Ser Lys Ala Phe Ser Lys Ala Phe Ser

5 10

Lys Ala Phe Ser Lys Ala

15

(2) INFORMATION FOR SEQ ID NO: 4:

(1) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 20 amino acids (B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(x) PUBLICATION INFORMATION:

(H) DOCUMENT NUMBER: W089/11290

(I) FILING DATE: 19-MAY-1989

(J) PUBLICATION DATE: 30-NOV-1989

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 4:

Ser Lys Ala Phe Ser Lys Ala Phe Ser Lys

5 10

Ala Phe Ser Lys Ala Phe Ser Lys Ala Phe

15 20

(2) INFORMATION FOR SEQ ID NO: 5:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 16 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(x) PUBLICATION INFORMATION:

(H) DOCUMENT NUMBER: W089/11290

(I) FILING DATE: 19-MAY-1989

(J) PUBLICATION DATE: 30-NOV-1989

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:5: Lys Ala Phe Ser Lys Ala Phe Ser Lys Ala

5 10

Phe Ser Lys Ala Phe Ser

15

(2) INFORMATION FOR SEQ ID NO: 6:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 23 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(A) NAME/KEY: Magainin I peptide.

(x) PUBLICATION INFORMATION:

(A) AUTHOR: Zasloff, Michael

(C) JOURNAL: Proc. Nat. Acad. Sci.

(D) VOLUME: 84

(F) PAGES: 5449-5453 (G) DATE: AUG - 1987

(H) DOCUMENT NUMBER: US 4810777

(I) FILING DATE: 04-MAR-1987

(J) PUBLICATION DATE: 07-MAR-1989

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 6: Gly Ile Gly Lys Phe Leu His Ser Ala Gly

5 10

Lys Phe Gly Lys Ala Phe Val Gly Glu Ile

15 20

Met Lys Ser

(2) INFORMATION FOR SEQ ID NO: 7:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 23 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(A) NAME/KEY: Magainin II peptide.

(x) PUBLICATION INFORMATION:

(A) AUTHOR: Zasloff, Michael

(C) JOURNAL: Proc. Nat. Acad. Sci.

(D) VOLUME: 84

(F) PAGES: 5449-5453

(G) DATE: AUG - 1987

(H) DOCUMENT NUMBER: US 4810777

(I) FILING DATE: 04-MAR-1987

(J) PUBLICATION DATE: 07-MAR-1989

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 7: Gly Ile Gly Lys Phe Leu His Ser Ala Lys

5 10

Lys Phe Gly Lys Ala Phe Val Gly Glu Ile

15 20

Met Asn Ser

(2) INFORMATION FOR SEQ ID NO: 8:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 22 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(A) NAME/KEY: Magainin III peptide.

(x) PUBLICATION INFORMATION: (A) AUTHOR: Zasloff, Michael

(C) JOURNAL: Proc. Nat. Acad. Sci.

(D) VOLUME: 84

(F) PAGES: 5449-5453

(G) DATE: AUG - 1987

(H) DOCUMENT NUMBER: US 4810777

(I) FILING DATE: 04-MAR-1987

(J) PUBLICATION DATE: 07-MAR-1989

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 8: Gly Ile Gly Lys Phe Leu His Ser Ala Lys

5 10

Lys Phe Gly Lys Ala Phe Val Gly Glu Ile

15 20

Met Asn

(2) INFORMATION FOR SEQ ID NO: 9:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 22 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

( ii) MOLECULE TYPE : peptide

(ix) FEATURE:

(A) NAME/KEY: magainin peptide.

(x) PUBLICATION INFORMATION:

(A) AUTHOR: Zasloff, Michael

(C) JOURNAL: Proc. Nat. Acad. Sci.

(D) VOLUME: 84

(F) PAGES: 5449-5453

(G) DATE: AUG - 1987

(H) DOCUMENT NUMBER: US 4810777

(I) FILING DATE: 04-MAR-1987

(J) PUBLICATION DATE: 07-MAR-1989

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 9: Ile Gly Lys Phe Leu His Ser Ala Lys Lys

5 10

Phe Gly Lys Ala Phe Val Gly Glu Ile Met

15 20

Asn Ser

(2) INFORMATION FOR SEQ ID NO: 10:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide (ix) FEATURE:

(A) NAME/KEY: magainin peptide.

(x) PUBLICATION INFORMATION:

(A) AUTHOR: Zasloff, Michael

(C) JOURNAL: Proc. Nat. Acad. Sci.

(D) VOLUME: 84

(F) PAGES: 5449-5453

(G) DATE: AUG - 1987

(H) DOCUMENT NUMBER: US 4810777

(I) FILING DATE: 04-MAR-1987

(J) PUBLICATION DATE: 07-MAR-1989

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 10: Gly Lys Phe Leu His Ser Ala Lys Lys Phe

5 10

Gly Lys Ala Phe Val Gly Glu Ile Met Asn

15 20

Ser

(2) INFORMATION FOR SEQ ID NO: 11:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 20 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(A) NAME/KEY: magainin peptide.

(x) PUBLICATION INFORMATION:

(A) AUTHOR: Zasloff, Michael

(C) JOURNAL: Proc. Nat. Acad. Sci. (D) VOLUME: 84

(F) PAGES: 5449-5453

(G) DATE: AUG - 1987

(H) DOCUMENT NUMBER: US 4810777

(I) FILING DATE: 04-MAR-1987

(J) PUBLICATION DATE: 07-MAR-1989

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 11: Lys Phe Leu His Ser Ala Lys Lys Phe Gly

5 10

Lys Ala Phe Val Gly Glu Ile Met Asn Ser

15 20

(2) INFORMATION FOR SEQ ID NO: 12:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(A) NAME/KEY: PGLa peptide.

(x) PUBLICATION INFORMATION:

(A) AUTHOR: Hoffman, et al.

(C) JOURNAL: EMBO J.

(D) VOLUME: 2

(F) PAGES: 711-714

(G) DATE: 1983

(A) AUTHOR: Andreu, et al.

(C) JOURNAL: Journal of Biochemistry

(D) VOLUME: 149

(F) PAGES: 531-535

(G) DATE: 1985

(A) AUTHOR: Gibson, et al.

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 5341-5349

(G) DATE: 1986

(A) AUTHOR: Giovannini, et al.

(C) JOURNAL: Biochem J.

(D) VOLUME: 243

(F) PAGES: 113-120

(G) DATE: 1987

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 12: Gly Met Ala Ser Lys Ala Gly Ala Ile Ala

5 10

Gly Lys Ile Ala Lys Val Ala Leu Lys Ala

15 20

Leu

(2) INFORMATION FOR SEQ ID NO: 13:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 25 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

( ii) MOLECULE TYPE : peptide

(ix) FEATURE:

(A) NAME/KEY: XPF peptide.

(x) PUBLICATION INFORMATION:

(A) AUTHOR: Hoffman, et al.1

(C) JOURNAL: EMBO J.

(D) VOLUME: 2

(F) PAGES: 711-714

(G) DATE: 1983

(A) AUTHOR: Andreu, et al . (C) JOURNAL: Journal of Biochemistry (D) VOLUME : 149

(F) PAGES: 531-535

(G) DATE: 1985

(A) AUTHOR: Gibson, et al.

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 5341-5349

(G) DATE: 1986

(A) AUTHOR: Giovannini, et al.

(C) JOURNAL: Biochem J.

(D) VOLUME: 243

(F) PAGES: 113-120

(G) DATE: 1987

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 13:

Gly Trp Ala Ser Lys Ile Gly Gln Thr Leu

5 10

Gly Lys Ile Ala Lys Val Gly Leu Lys Glu

15 20 Leu Ile Gln Pro Lys

25

(2) INFORMATION FOR SEQ ID NO: 14:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 27 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(A) NAME/KEY: CPF peptide.

(x) PUBLICATION INFORMATION:

(A) AUTHOR: Richter, K.

Egger, R.

Kreil

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 3676-3680

(G) DATE: 1986

(A) AUTHOR: Wakabayashi, T.

Kato, H.

Tachibaba, S.

(C) JOURNAL: Nucleic Acids Research

(D) VOLUME: 13

(F) PAGES: 1817-1828

(G) DATE: 1985

(A) AUTHOR: Gibson, B.W.

Poulter, L.

Williams, D.H.

Maggio, J.E.

(C) JOURNAL: J. Biol. Chem. (D) VOLUME : 261

(F) PAGES: 5341-5349

(G) DATE: 1986

(H) DOCUMENT NUMBER: W090/04407

(I) FILING DATE: 16-OCT-1989

(J) PUBLICATION DATE: 03-MAY-1990

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 14:

Gly Phe Gly Ser Phe Leu Gly Leu Ala Leu

5 10

Lys Ala Ala Leu Lys Ile Gly Ala Asn Ala

15 20

Leu Gly Gly Ala Pro Gln Gln

25

(2) INFORMATION FOR SEQ ID NO: 15:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 27 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(A) NAME/KEY : CPF peptide.

(x) PUBLICATION INFORMATION:

(A) AUTHOR: Richter, K

Egger, R.

Kreil

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 3676-3680

(G) DATE: 1986

(A) AUTHOR: Wakabayashi, T.

Kato, H.

Tachibaba, S.

(C) JOURNAL: Nucleic Acids Research

(D) VOLUME: 13

(F) PAGES: 1817-1828

(G) DATE: 1985

(A) AUTHOR: Gibson, B.W.

Poulter, L.

Williams, D.H.

Maggio, J.E.

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 5341-5349

(G) DATE: 1986

(H) DOCUMENT NUMBER: W090/04407

(I) FILING DATE: 16-OCT-1989

(J) PUBLICATION DATE: 03-MAY-1990 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 15:

Gly Leu Ala Ser Phe Leu Gly Lys Ala Leu

5 10

Lys Ala Gly Leu Lys Ile Gly Ala His Leu

15 20

Leu Gly Gly Ala Pro Gln Gln

25

(2) INFORMATION FOR SEQ ID NO: 16:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 27 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

( ii) MOLECULE TYPE : peptide

( ix) FEATURE :

(A) NAME/KEY: CPF peptide.

(x) PUBLICATION INFORMATION:

(A) AUTHOR: Richter, K.

Egger, R .

Kreil

(C) JOURNAL: J. Biol . Chem.

(D) VOLUME : 261

(F) PAGES: 3676-3680

(G) DATE: 1986

(A) AUTHOR: Wakabayashi, T.

Kato, H.

Tachibaba, S.

(C) JOURNAL: Nucleic Acids Research

(D) VOLUME: 13

(F) PAGES: 1817-1828

(G) DATE: 1985

(A) AUTHOR: Gibson, B.W.

Poulter, L.

Williams, D.H.

Maggio, J.E.

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 5341-5349

(G) DATE: 1986

(H) DOCUMENT NUMBER: W090/04407

(I) FILING DATE: 16-0CT-1989

(J) PUBLICATION DATE: 03-MAY-1990

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 16:

Gly Leu Ala Ser Leu Leu Gly Lys Ala Leu

5 10

Lys Ala Gly Leu Lys Ile Gly Thr His Phe

15 20

Leu Gly Gly Ala Pro Gln Gln

25 (2) INFORMATION FOR SEQ ID NO: 17:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 27 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

( ix) FEATURE :

(A) NAME/KEY: CPF peptide.

(x) PUBLICATION INFORMATION:

(A) AUTHOR: Richter, K.

Egger, R.

Kreil

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES : 3676-3680

(G) DATE: 1986

(A) AUTHOR: Wakabayashi, T.

Kato, H.

Tachibaba, S.

(C) JOURNAL: Nucleic Acids Research

(D) VOLUME: 13

(F) PAGES: 1817-1828

(G) DATE: 1985

(A) AUTHOR: Gibson, B.W.

Poulter, L.

Williams, D.H.

Maggio, J.E.

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 5341-5349

(G) DATE: 1986

(H) DOCUMENT NUMBER: W090/04407

(I) FILING DATE: 16-OCT-1989

(J) PUBLICATION DATE: 03-MAY-1990

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 17: Gly Leu Ala Ser Leu Leu Gly Lys Ala Leu

5 10

Lys Ala Thr Leu Lys Ile Gly Thr His Phe

15 20

Leu Gly Gly Ala Pro Gln Gln

25

(2) INFORMATION FOR SEQ ID NO: 18:

(1) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 27 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(A) NAME/KEY : CPF peptide.

(x) PUBLICATION INFORMATION:

(A) AUTHOR: Richter, K.

Egger, R.

Kreil

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES : 3676-3680

(G) DATE: 1986

(A) AUTHOR: Wakabayashi, T.

Kato, H.

Tachibaba, S.

(C) JOURNAL: Nucleic Acids Research

(D) VOLUME: 13

(F) PAGES: 1817-1828

(G) DATE: 1985

(A) AUTHOR: Gibson, B.W.

Poulter, L.

Williams, D.H.

Maggio, J.E.

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 5341-5349

(G) DATE: 1986

(H) DOCUMENT NUMBER: W090/04407

(I) FILING DATE: 16-OCT-1989

(J) PUBLICATION DATE: 03-MAY-1990

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 18: Gly Phe Ala Ser Phe Leu Gly Lys Ala Leu

5 10

Lys Ala Ala Leu Lys Ile Gly Ala Asn Met

15 20

Leu Gly Gly Thr Pro Gln Gln

25

(2) INFORMATION FOR SEQ ID NO: 19:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 27 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(A) NAME/KEY: CPF peptide.

(x) PUBLICATION INFORMATION:

(A) AUTHOR: Richter, K. Egger, R.

Kreil

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES : 3676-3680

(G) DATE: 1986

(A) AUTHOR: Wakabayashi, T.

Kato, H.

Tachibaba, S.

(C) JOURNAL: Nucleic Acids Research

(D) VOLUME: 13

(F) PAGES: 1817-1828

(G) DATE: 1985

(A) AUTHOR: Gibson, B.W.

Poulter, L.

Williams, D.H.

Maggio, J.E.

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 5341-5349

(G) DATE: 1986

(H) DOCUMENT NUMBER: W090/04407

(I) FILING DATE: 16-0CT-1989

(J) PUBLICATION DATE: 03-MAY-1990

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 19: Gly Phe Gly Ser Phe Leu Gly Lys Ala Leu

5 10

Lys Ala Ala Leu Lys Ile Gly Ala Asn Ala

15 20

Leu Gly Gly Ala Pro Gln Gln

25

(2) INFORMATION FOR SEQ ID NO: 20:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 27 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(A) NAME/KEY: CPF peptide.

(x) PUBLICATION INFORMATION:

(A) AUTHOR: Richter, K.

Egger, R.

Kreil

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 3676-3680

(G) DATE: 1986

(A) AUTHOR: Wakabayashi, T. Kato, H.

Tachibaba, S.

(C) JOURNAL: Nucleic Acids Research

(D) VOLUME: 13

(F) PAGES : 1817-1828

(G) DATE: 1985

(A) AUTHOR: Gibson, B.W.

Poulter, L.

Williams, D.H.

Maggio, J.E.

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES : 5341-5349

(G) DATE: 1986

(H) DOCUMENT NUMBER: W090/04407

(I) FILING DATE: 16-OCT-1989

(J) PUBLICATION DATE: 03-MAY-1990

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 20: Gly Phe Gly Ser Phe Leu Gly Lys Ala Leu

5 10

Lys Ala Ala Leu Lys Ile Gly Ala Asn Ala

15 20

Leu Gly Gly Ser Pro Gln Gln

25

(2) INFORMATION FOR SEQ ID NO: 21:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 27 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

ix) FEATURE:

(A) NAME/KEY : CPF peptide. x) PUBLICATION INFORMATION:

(A) AUTHOR: Richter, K.

Egger, R.

Kreil

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 3676-3680

(G) DATE: 1986

(A) AUTHOR: Wakabayashi, T.

Kato, H.

Tachibaba, S.

(C) JOURNAL: Nucleic Acids Research

(D) VOLUME: 13

(F) PAGES: 1817-1828

(G) DATE: 1985

(A) AUTHOR: Gibson, B.W. Poulter, L.

Williams, D.H.

Maggio, J.E.

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 5341-5349

(G) DATE: 1986

(H) DOCUMENT NUMBER: W090/04407

(I) FILING DATE: 16-0CT-1989

(J) PUBLICATION DATE: 03-MAY-1990

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 21: Gly Phe Ala Ser Phe Leu Gly Lys Ala Leu

5 10

Lys Ala Ala Leu Lys Ile Gly Ala Asn Leu

15 20

Leu Gly Gly Thr Pro Gln Gln

25

(2) INFORMATION FOR SEQ ID NO: 22:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 27 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(A) NAME/KEY: CPF peptide.

(x) PUBLICATION INFORMATION:

(A) AUTHOR: Richter, K.

Egger, R.

Kreil

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 3676-3680

(G) DATE: 1986

(A) AUTHOR: Wakabayashi, T.

Kato, H.

Tachibaba, S.

(C) JOURNAL: Nucleic Acids Research

(D) VOLUME: 13

(F) PAGES: 1817-1828

(G) DATE: 1985

(A) AUTHOR: Gibson, B.W.

Poulter, L.

Williams, D.H.

Maggio, J.E.

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 5341-5349

(G) DATE: 1986 (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 22: Gly Phe Ala Ser Phe Leu Gly Lys Ala Leu

5 10

Lys Ala Ala Leu Lys Ile Gly Ala Asn Ala

15 20

Leu Gly Gly Ala Pro Gln Gln

25

(2) INFORMATION FOR SEQ ID NO: 23:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 27 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(A) NAME/KEY: CPF peptide.

(x) PUBLICATION INFORMATION:

(A) AUTHOR: Richter, K.

Egger, R.

Kreil

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 3676-3680

(G) DATE: 1986

(A) AUTHOR: Wakabayashi, T.

Kato, H.

Tachibaba, S.

(C) JOURNAL: Nucleic Acids Research

(D) VOLUME: 13

(F) PAGES: 1817-1828

(G) DATE: 1985

(A) AUTHOR: Gibson, B.W.

Poulter, L.

Williams, D.H.

Maggio, J.E.

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 5341-5349

(G) DATE: 1986

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 23: Gly Phe Ala Ser Phe Leu Gly Lys Ala Leu

5 10

Lys Ala Ala Leu Lys Ile Gly Ala Asn Met

15 20

Leu Gly Gly Ala Pro Gln Gln

25

(2) INFORMATION FOR SEQ ID NO: 24:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 27 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

( ii) MOLECULE TYPE : peptide

(ix) FEATURE:

(A) NAME/KEY: CPF peptide.

(x) PUBLICATION INFORMATION:

(A) AUTHOR: Richter, K.

Egger, R.

Kreil

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 3676-3680

(G) DATE: 1986

(A) AUTHOR: Wakabayashi, T.

Kato, H.

Tachibaba, S.

(C) JOURNAL: Nucleic Acids Research

(D) VOLUME: 13

(F) PAGES: 1817-1828

(G) DATE: 1985

(A) AUTHOR: Gibson, B.W.

Poulter, L.

Williams, D.H.

Maggio, J.E.

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 5341-5349

(G) DATE: 1986

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 24: Gly Phe Gly Ser Phe Leu Gly Lys Ala Leu

5 10

Lys Ala Ala Leu Lys Ile Gly Ala Asn Ala

15 20

Leu Gly Gly Ser Leu Gln Gln

25

(2) INFORMATION FOR SEQ ID NO: 25:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 27 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(A) NAME/KEY: CPF peptide. (x) PUBLICATION INFORMATION:

(A) AUTHOR: Richter, K.

Egger, R.

Kreil

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 3676-3680

(G) DATE: 1986

(A) AUTHOR: Wakabayashi, T.

Kato, H.

Tachibaba, S.

(C) JOURNAL: Nucleic Acids Research

(D) VOLUME: 13

(F) PAGES : 1817-1828

(G) DATE: 1985

(A) AUTHOR: Gibson, B.W.

Poulter, L.

Williams, D.H.

Maggio, J.E.

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES : 5341-5349

(G) DATE: 1986

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 25:

Gly Phe Gly Ser Phe Leu Gly Lys Ala Leu

5 10

Lys Ala Gly Leu Lys Ile Gly Thr Asn Phe

15 20 Leu Gly Gly Ala Pro Gln Gln

25

(2) INFORMATION FOR SEQ ID NO: 26:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 27 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

( ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(A) NAME/KEY: CPF peptide.

(x) PUBLICATION INFORMATION:

(A) AUTHOR: Richter, K

Egger, R.

Kreil

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 3676-3680

(G) DATE: 11998866

(A) AUTHOR: Wakabayashi, T. Kato, H.

Tachibaba, S.

(C) JOURNAL: Nucleic Acids Research

(D) VOLUME: 13

(F) PAGES: 1817-1828

(G) DATE: 1985

(A) AUTHOR: Gibson, B.W.

Poulter, L.

Williams, D.H.

Maggio, J.E.

(C) JOURNAL: J. Biol. Chem.

(D) VOLUME: 261

(F) PAGES: 5341-5349

(G) DATE: 1986

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 26: Gly Leu Ala Ser Leu Leu Gly Lys Ala Leu

5 10

Lys Ala Ala Leu Lys Ile Gly Ala Asn Ala

15 20

Leu Gly Gly Ser Pro Gln Gln

25

(2) INFORMATION FOR SEQ ID NO: 27:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 27: Lys Ile Ala Gly Lys Ile Ala Lys Ile Ala

5 10

Gly Lys Ile Ala Lys Ile Ala Gly Lys Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 28:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 28:

Lys Ile Ala Lys Ile Ala Gly Lys Ile Ala

5 10

Lys Ile Ala Gly Lys Ile Ala Lys Ile Ala

15 20

Gly (2) INFORMATION FOR SEQ ID NO: 29:

(i) SEQUENCE CHARACTERISTICS: :

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 29:

Lys Ile Ala Gly Lys Ile Gly Lys Ile Ala

5 10

Gly Lys Ile Gly Lys Ile Ala Gly Lys Ile

15 20

Gly

(2) INFORMATION FOR SEQ ID NO: 30:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 30:

Lys Leu Ala Gly Lys Leu Ala Lys Leu Ala

5 10

Gly Lys Leu Ala Lys Leu Ala Gly Lys Leu

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 31:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 31: Lys Phe Ala Gly Lys Phe Ala Lys Phe Ala

5 10

Gly Lys Phe Ala Lys Phe Ala Gly Lys Phe

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 32:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid (C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 32:

Lys Ala Leu Ser Lys Ala Leu Lys Ala Leu

5 10

Ser Lys Ala Leu Lys Ala Leu Ser Lys Ala

15 20

Leu

(2) INFORMATION FOR SEQ ID NO: 33:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 33:

Lys Leu Leu Lys Ala Leu Gly Lys Leu Leu

5 10

Lys Ala Leu Gly Lys Leu Leu Lys Ala Leu

15 20

Gly

(2) INFORMATION FOR SEQ ID NO: 34:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 34:

Lys Ala Ile Gly Lys Ala Ile Lys Ala Ile

5 10

Gly Lys Ala Ile Lys Ala Ile Gly Lys Ala

15 20

Ile

(2) INFORMATION FOR SEQ ID NO: 35:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 35: Gly Ile Ala Lys Ile Ala Lys Gly Ile Ala

5 10

Lys Ile Ala Lys Gly Ile Ala Lys Ile Ala

15 20

Lys

(2) INFORMATION FOR SEQ ID NO: 36:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 36: Lys Ile Ala Lys Ile Phe Gly Lys Ile Ala

5 10

Lys Ile Phe Gly Lys Ile Ala Lys Ile Phe

15 20

Gly

(2) INFORMATION FOR SEQ ID NO: 37:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 37: Gly Ile Ala Arg Ile Ala Lys Gly Ile Ala

5 10

Arg Ile Ala Lys Gly Ile Ala Arg Ile Ala

15 20

Lys

(2) INFORMATION FOR SEQ ID NO: 38:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 38: Lys Phe Ala Arg Ile Ala Gly Lys Phe Ala

5 10

Arg Ile Ala Gly Lys Phe Ala Arg Ile Ala

15 20

Gly (2) INFORMATION FOR SEQ ID NO: 39:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 39:

Gly Phe Ala Lys Ile Ala Lys Gly Phe Ala

5 10

Lys Ile Ala Lys Gly Phe Ala Lys Ile Ala

15 20

Lys

(2) INFORMATION FOR SEQ ID NO:40:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is ornithine

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:40:

Lys Ile Ala Gly Xaa Ile Ala Lys Ile Ala

5 10

Gly Xaa Ile Ala Lys Ile Ala Gly Xaa Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 41:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 41: Lys Ile Ala Arg Ile Ala Gly Lys Ile Ala

5 10

Arg Ile Ala Gly Lys Ile Ala Arg Ile Ala

15 20

Gly

(2) INFORMATION FOR SEQ ID NO: 42:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid (C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is ornithine

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 42:

Xaa Ile Ala Gly Lys Ile Ala Xaa Ile Ala

5 10

Gly Lys Ile Ala Xaa Ile Ala Gly Lys Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 43:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 43:

Gly Ile Ala Arg Ile Phe Lys Gly Ile Ala

5 10

Arg Ile Phe Lys Gly Ile Ala Arg Ile Phe

15 20

Lys

(2) INFORMATION FOR SEQ ID NO: 44:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is norleucine.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 44:

Lys Xaa Ala Gly Lys Xaa Ala Lys Xaa Ala

5 10

Gly Lys Xaa Ala Lys Xaa Ala Gly Lys Xaa

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 45:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid (C) STRANDEDNESS:

(D) TOPOLOGY: linear

( ii) MOLECULE TYPE : peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is norleucine.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 45:

Lys Xaa Ala Gly Lys Ile Ala Lys Xaa Ala

5 10

Gly Lys Ile Ala Lys Xaa Ala Gly Lys Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 46:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

( ii) MOLECULE TYPE : peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is norleucine.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:46:

Lys Ile Ala Gly Lys Xaa Ala Lys Ile Ala

5 10

Gly Lys Xaa Ala Lys Ile Ala Gly Lys Xaa

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 47:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

( ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is norvaline.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:47:

Lys Xaa Ala Gly Lys Xaa Ala Lys Xaa Ala

5 10

Gly Lys Xaa Ala Lys Xaa Ala Gly Lys Xaa

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 48: ( i) SEQUENCE CHARACTERISTICS :

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS :

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is norvaline.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:48:

Lys Xaa Ala Gly Lys Ile Ala Lys Xaa Ala

5 10

Gly Lys Ile Ala Lys Xaa Ala Gly Lys Xaa

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 49:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:49:

Lys Leu Leu Ser Lys Leu Gly Lys Leu Leu

5 10

Ser Lys Leu Gly Lys Leu Leu Ser Lys Leu

15 20

Gly

(2) INFORMATION FOR SEQ ID NO: 50:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:50:

Lys Leu Leu Ser Lys Phe Gly Lys Leu Leu

5 10

Ser Lys Phe Gly Lys Leu Leu Ser Lys Phe

15 20

Gly

(2) INFORMATION FOR SEQ ID NO:51:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid (C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is norvaline.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 51: Lys Ile Ala Gly Lys Xaa Ala Lys Ile Ala

5 10

Gly Lys Xaa Ala Lys Ile Ala Gly Lys Xaa

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 52:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:52: His Ile Ala Gly His Ile Ala His Ile Ala

5 10

Gly His Ile Ala His Ile Ala Gly His Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO:53:

(1) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:53: Ala Gly Lys Ile Ala Lys Ile Ala Gly Lys

5 10

Ile Ala Lys Ile Ala Gly Lys Ile Ala Lys

15 20

Ile

(2) INFORMATION FOR SEQ ID NO:54:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 54: Ile Ala Lys Ile Ala Gly Lys Ile Ala Lys

5 10

Ile Ala Gly Lys Ile Ala Lys Ile Ala Gly

15 20

Lys

(2) INFORMATION FOR SEQ ID NO: 55:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO-55: Lys Ile Ala Gly Arg Ile Ala Lys Ile Ala

5 10

Gly Arg Ile Ala Lys Ile Ala Gly Arg Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO:56:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 56: Arg Ile Ala Gly Arg Ile Ala Arg Ile Ala

5 10

Gly Arg Ile Ala Arg Ile Ala Gly Arg Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO:57:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:57: Lys Val Ala Gly Lys Ile Ala Lys Val Ala

5 10 Gly Lys Ile Ala Lys Val Ala Gly Lys Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 58:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 58: Lys Ile Ala Gly Lys Val Ala Lys Ile Ala

5 10

Gly Lys Val Ala Lys Ile Ala Gly Lys Val

15 20

Ala

(2) INFORMATION FOR SEQ ID NO:59:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:59: Ala Lys Ile Ala Gly Lys Ile Ala Lys Ile

5 10

Ala Gly Lys Ile Ala Lys Ile Ala Gly Lys

15 20

Ile

(2) INFORMATION FOR SEQ ID NO: 60:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is ornithine.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 60:

Xaa Ile Ala Gly Xaa Ile Ala Xaa Ile Ala

5 10

Gly Xaa Ile Ala Xaa Ile Ala Gly Xaa Ile

15 20

Ala (2) INFORMATION FOR SEQ ID NO: 61:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 61:

Lys Phe Ala Gly Lys Ile Ala Lys Phe Ala

5 10

Gly Lys Ile Ala Lys Phe Ala Gly Lys Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 62:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 62:

Lys Ile Ala Gly Lys Phe Ala Lys Ile Ala

5 10

Gly Lys Phe Ala Lys Ile Ala Gly Lys Phe

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 63:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is cyclohexylalanine.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 63:

Lys Xaa Ala Gly Lys Ile Ala Lys Xaa Ala

5 10

Gly Lys Ile Ala Lys Xaa Ala Gly Lys Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 64:

(i) SEQUENCE CHARACTERISTICS: (A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is norleucine.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 64:

Lys Xaa Ala Lys Ile Ala Gly Lys Xaa Ala

5 10

Lys Ile Ala Gly Lys Xaa Ala Lys Ile Ala

15 20

Gly

(2) INFORMATION FOR SEQ ID NO: 65:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 21 AMINO ACIDS

(B) TYPE: amino acids

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 65:

Arg Ile Ala Gly Lys Ile Ala Arg Ile Ala

5 10

Gly Lys Ile Ala Arg Ile Ala Gly Lys Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 66:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is homoarginine.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 66:

Xaa Ile Ala Gly Xaa Ile Ala Xaa Ile Ala

5 10

Gly Xaa Ile Ala Xaa Ile Ala Gly Xaa Ile

15 20

Ala

(2)INFORMATION FOR SEQ ID NO:67:

(i) SEQUENCE CHARACTERISTICS:: (A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE: Xaa is p-aminophenylalanine

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 67: Xaa Ile Ala Gly Lys Ile Ala Xaa Ile Ala

5 10

Gly Lys Ile Ala Xaa Ile Ala Gly Lys Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 68:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE: Xaa is p-aminophenylalanine

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 68: Lys Ile Ala Gly Xaa Ile Ala Lys Ile Ala

5 10

Gly Xaa Ile Ala Lys Ile Ala Gly Xaa Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 69:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO:69: Lys Leu Ala Ser Lys Ala Gly Lys Ile Ala Gly

5 10

Lys Ile Ala Lys Val Ala Leu Lys Ala Leu

15 20

(2) INFORMATION FOR SEQ ID NO: 70:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: (D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is ornithine.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 70:

Lys Ile Ala Gly Lys Ile Ala Lys Ile Ala Gly

5 10

Xaa Ile Ala Lys Ile Ala Gly Lys Ile Ala

15 20

(2) INFORMATION FOR SEQ ID NO: 71:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 71:

Lys Ile Ala Gly Lys Ile Ala Lys Ile Ala

5 10

Gly Arg Ile Ala Lys Ile Ala Gly Lys Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 72:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is norleucine.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 72:

Lys Ile Ala Gly Lys Ile Ala Lys Ile Ala

5 10

Gly Xaa Ile Ala Lys Ile Ala Gly Lys Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 73:

(1) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear ( ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is norvaline.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 73:

Lys Ile Ala Gly Lys Ile Ala Lys Ile Ala

5 10

Gly Xaa Ile Ala Lys Ile Ala Gly Lys Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 74:

(1) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is ornithine.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 74:

Lys Phe Ala Gly Lys Phe Ala Lys Phe Ala Gly

5 10

Xaa Phe Ala Lys Phe Ala Gly Lys Phe Ala

15 20

(2) INFORMATION FOR SEQ ID NO: 75:

(1) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is ornithine.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 75:

Lys Ile Ala Gly Lys Phe Ala Lys Ile Ala

5 10

Gly Xaa Phe Ala Lys Ile Ala Gly Lys Phe

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 76:

(1) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: (D) TOPOLOGY: linear

( ii) MOLECULE TYPE : peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa at residues 6, 13, and 20

is norleucine; Xaa at residue

12 is ornlthine.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 76:

Lys Ile Ala Gly Lys Xaa Ala Lys Ile Ala

5 10

Gly Xaa Xaa Ala Lys Ile Ala Gly Lys Xaa

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 77:

(1) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 77:

Lys Met Ala Ser Lys Ala Gly Lys Ile Ala

5 10

Gly Lys Ile Ala Lys Val Ala Leu Lys Ala

15 20

Leu

(2) INFORMATION FOR SEQ ID NO: 78:

(1) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 78

Lys Ile Ala Ser Lys Ala Gly Lys Ile Ala

5 10

Gly Lys Ile Ala Lys Val Ala Leu Lys Ala Leu

15 20

(2) INFORMATION FOR SEQ ID NO: 79:

(1) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS: (D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is norleucine.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 79:

Lys Ile Ala Ser Lys Ala Gly Lys Xaa Ala

5 10

Gly Lys Ile Ala Lys Val Ala Leu Lys Ala Leu

15 20

(2) INFORMATION FOR SEQ ID NO: 80:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is norleucine.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 80:

Lys Leu Ala Ser Lys Ala Gly Lys Xaa Ala

5 10

Gly Lys Ile Ala Lys Val Ala Leu Lys Ala

15 20

Leu

(2) INFORMATION FOR SEQ ID NO: 81:

(1) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is norleucine.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 81:

Lys Xaa Ala Ser Lys Ala Gly Lys Xaa Ala

5 10

Gly Lys Ile Ala Lys Val Ala Leu Lys Ala Leu

15 20

(2) INFORMATION FOR SEQ ID NO: 82:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid (C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE:

(D) OTHER INFORMATION: Xaa is p-aminophenylalanine.

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 82: Lys Ile Ala Gly Lys Ile Ala Lys Ile Ala

5 10

Gly Xaa Ile Ala Lys Ile Ala Gly Lys Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 83:

(1) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 83: Lys Ile Ala Gly Ala Ile Ala Lys Ile Ala

5 10

Gly Lys Ile Ala Lys Ile Ala Gly Lys Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 84:

(1) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 84: Lys Ile Ala Gly Lys Ile Ala Lys Ile Ala

5 10

Gly Ala Ile Ala Lys Ile Ala Gly Lys Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 85:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 85: Lys Ile Ala Gly Lys Ile Ala Lys Ile Ala

5 10

Gly Lys Ile Ala Lys Ile Ala Gly Ala Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 86:

(1) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 86:

Lys Ile Ala Lys Lys Ile Ala Lys Ile Ala

5 10

Lys Lys Ile Ala Lys Ile Ala Lys Lys Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 87:

(1) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 87:

Lys Phe Ala Lys Lys Phe Ala Lys Phe Ala

5 10

Lys Lys Phe Ala Lys Phe Ala Lys Lys Phe

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 88:

(1) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 88: Lys Phe Ala Lys Lys Ile Ala Lys Phe Ala

5 10

Lys Lys Ile Ala Lys Phe Ala Lys Lys Ile Ala

15 20

(2) INFORMATION FOR SEQ ID NO: 89:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 89:

Ala Ile Ala Gly Lys Ile Ala Lys Ile Ala

5 10

Gly Lys Ile Ala Lys Ile Ala Gly Lys Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 90:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 90: Lys Ile Ala Gly Lys Ile Ala Ala Ile Ala

5 10

Gly Lys Ile Ala Lys Ile Ala Gly Lys Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 91:

(1) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 91: Lys Ile Ala Gly Lys Ile Ala Lys Ile Ala

5 10

Gly Lys Ile Ala Ala Ile Ala Gly Lys Ile

15 20

Ala

(2) INFORMATION FOR SEQ ID NO: 92:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 21 amino acids (B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 92:

Gly Met Ala Ser Lys Ala Gly Lys Ile Ala

5 10

Gly Lys Ile Ala Lys Val Ala Leu Lys Ala

15 20

Leu

(2) INFORMATION FOR SEQ ID NO: 93:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 26 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(vi) ORIGINAL SOURCE

(A) ORGANISM: Apis mellifera

(vii) FEATURE

(A) NAME/KEY: melittin peptide

(x) PUBLICATION INFORMATION:

(A) AUTHORS: Habermann, E.

Jentsch, J.

(B) TITLE: Sequenzanalyse des Melittins aus den tryptischen and

peptischen

Spaltstucken

(C) JOURNAL: Hoppe-Seyler's Zeitschrift

Physio1. Chem

(D) VOLUME: 348

(F) PAGES: 37-50

(G) DATE: 1987

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 93:

Gly Ile Gly Ala Val Leu Lys Val Leu

5

Thr Thr Gly Leu Pro Ala Leu Ile Ser Trp

10 15

Ile Lys Arg Lys Arg Gln Gln

20 25

(2) INFORMATION FOR SEQ ID NO: 94:

(1) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 11 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 94: Leu Lys Lys Leu Lys Lys Leu Leu Lys Leu

5 10

Leu

(2) INFORMATION FOR SEQ ID NO: 95:

(i) SEQUENCE CHARACTERISTICS:

(A) LENGTH: 12 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 95: Leu Leu Lys Lys Leu Lys Lys Leu Leu Lys

5 10

Leu Leu

(2) INFORMATION FOR SEQ ID NO: 96:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 13 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 96:

Lys Leu Leu Lys Lys Leu Lys Lys Leu Leu

5 10

Lys Leu Leu

(2) INFORMATION FOR SEQ ID NO: 97:

(1) SEQUENCE CHARACTERISTICS: :

(A) LENGTH: 14 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 97:

Lys Lys Leu Leu Lys Lys Leu Lys Lys Leu

5 10

Leu Lys Leu Leu

(2) INFORMATION FOR SEQ ID NO: 98:

(1) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 16 amino acids (B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) SEQUENCE DESCRIPTION: SEQ ID NO: 98: Lys Lys Leu Leu Lys Lys Leu Lys Lys Leu Leu Lys Lys Leu

5 10

Arg Arg

15

(2) INFORMATION FOR SEQ ID NO: 99:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 16 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 99:

Lys Leu Lys Lys Leu Leu Lys Lys Leu Lys

5 10

Lys Leu Leu Lys Leu Leu

15

(2) INFORMATION FOR SEQ ID NO: 100:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 15 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 100:

Leu Lys Lys Leu Leu Lys Lys Leu Lys Lys

5 10

Leu Leu Lys Lys Asn

15

(2) INFORMATION FOR SEQ ID NO: 101:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 15 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(ix) FEATURE: (D) OTHER INFORMATION: Xaa is homoserine. (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 101:

Leu Lys Lys Leu Leu Lys Lys Leu Lys Lys

5 10

Leu Leu Lys Lys Xaa

15

(2) INFORMATION FOR SEQ ID NO: 102:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 18 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 102:

Leu Lys Leu Leu Lys Lys Leu Leu Lys Lys

5 10

Asn Lys Lys Leu Leu Lys Lys Leu

15

(2) INFORMATION FOR SEQ ID NO: 103:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 18 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 103:

Leu Lys Leu Leu Lys Lys Leu Leu Lys Lys

5 10

Pro Lys Lys Leu Leu Lys Lys Leu

15

(2) INFORMATION FOR SEQ ID NO: 104:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 22 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide (xi) SEQUENCE DESCRIPTION: SEQ ID NO: 104:

Leu Leu Lys Lys Leu Lys Lys Leu Leu Lys

5 10

Lys Leu Gln Gly Pro Pro Gln Gly Gln Ser

15 20

Pro Gln

(2) INFORMATION FOR SEQ ID NO: 105:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 20 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 105:

Leu Ala Ser Lys Ala Gly Ala Ile Ala Gly

5 10

Lys Ile Ala Lys Lys Leu Leu Lys Lys Leu

15 20

(2) INFORMATION FOR SEQ ID NO: 106:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 7 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION:SEQ ID NO: 106: Leu Lys Lys Leu Lys Lys Leu

5

(2) INFORMATION FOR SEQ ID NO: 107:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 8 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 107: Leu Leu Lys Lys Leu Lys Lys Leu

5

(2) INFORMATION FOR SEQ ID NO: 108:

(i) SEQUENCE CHARACTERISTICS: :

(A) LENGTH: 9 amino acids

(B) TYPE: amino acid (C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 108: Lys Leu Leu Lys Lys Leu Lys Lys Leu

5

(2) INFORMATION FOR SEQ ID NO: 109:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 10 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 109: Lys Lys Leu Leu Lys Lys Leu Lys Lys Leu

5 10

(2) INFORMATION FOR SEQ ID NO: 110:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 11 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 110: Leu Lys Lys Leu Leu Lys Lys Leu Lys Lys Leu

5 10

(2) INFORMATION FOR SEQ ID NO: 111:

(1) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 11 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 111: Ala Lys Lys Leu Leu Lys Lys Leu Lys Lys Leu

5 10

(2) INFORMATION FOR SEQ ID NO: 112:

(1) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 11 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 112: Leu Lys Lys Leu Lys Lys Leu Leu Lys Lys Leu

5 10

(2) INFORMATION FOR SEQ ID NO: 113:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 12 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 113: Leu Leu Lys Lys Leu Lys Lys Leu Leu Lys Leu Leu

5 10

(2) INFORMATION FOR SEQ ID NO: 114:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 13 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 114: Lys Leu Leu Lys Lys Leu Lys Lys Leu Leu Lys Lys

5 10

Leu

(2) INFORMATION FOR SEQ ID NO: 115:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 14 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 115: Lys Lys Leu Leu Lys Lys Leu Lys Lys Leu Leu

5 10

Lys Lys Leu

(2) INFORMATION FOR SEQ ID NO: 116:

(i) SEQUENCE .CHARACTERISTICS: :

(A) LENGTH: 15 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear (ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 116: Leu Lys Lys Leu Leu Lys Lys Leu Lys Lys Leu Leu

5 10

Lys Lys Leu

15

(2) INFORMATION FOR SEQ ID NO: 117:

(i) SEQUENCE CHARACTERISTICS: :

(A) LENGTH: 14 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION: SEQ ID NO: 117: Leu Lys Lys Leu Leu Lys Lys Leu Lys Lys Leu

5 10

Leu Lys Arg

(2) INFORMATION FOR SEQ ID NO: 118:

(i) SEQUENCE CHARACTERISTICS::

(A) LENGTH: 14 amino acids

(B) TYPE: amino acid

(C) STRANDEDNESS:

(D) TOPOLOGY: linear

(ii) MOLECULE TYPE: peptide

(xi) SEQUENCE DESCRIPTION:SEQ ID NO: 118: Lys Ile Ala Lys Lys Ile Ala Lys Ile Ala

5 10

Lys Lys Ile Ala

Claims (53)

WHAT IS CLAIMED IS:

1. A composition for inhibiting growth of a target cell, virus, or virally-infected cell, comprising:

(a) a peptide or protein wherein the N-terminal amino acid is substituted, said peptide or protein having the formula:

W

T - N - X, wherein X is a biologically active peptide or protein, said peptide or protein being an ion channel-forming peptide or protein, N is the nitrogen of the N-terminal amino group, T is a lipophilic moiety, and W is T or hydrogen; and

(b) an acceptable pharmaceutical carrier, wherein said peptide or protein is present in an amount effective to inhibit growth of a target cell, virus, or virally-infected cell.

2. The composition of Claim 1 wherein W is hydrogen.

3. The composition of Claim 2 wherein T is:

, wherein R is a hydrocarbon having at least 2 and no more than 16 carbon atoms.

4. The composition of Claim 3 wherein R is an alkyl group.

5. The composition of Claim 4 wherein R is CH₃(CH₂)_n-, wherein n is from 1 to 14.

6. The composition of Claim 5 wherein n is from 4 to 9.

7. The composition of Claim 6 wherein n is 6.

8. The composition of Claim 3 wherein R is:

0 - (CH₂)_z-, wherein z is from 0 to 6.

9. The composition of Claim 9 wherein z is 1 or 2.

10. The composition of Claim 1 wherein X is a magainin peptide.

11. The composition of Claim 1 wherein X is a PGLa peptide.

12. The composition of Claim 1 wherein X is an XPF peptide.

13. The composition of Claim 1 wherein X is a CPF peptide.

14. The composition of Claim 1 wherein X is a cecropin.

15. The composition of Claim 1 wherein X is a sarcotoxin.

16. The composition of Claim 1 wherein X includes one of the following basic structures X₃₁ through X₃₇, wherein:

X₃₁ is -[R₃₁-R₃₂-R₃₂-R₃₃-R₃₁-R₃₂-R₃₂]_n-;

X₃₂ is -[R₃₂-R₃₂-R₃₃-R₃₁-R₃₂-R₃₂-R₃₁]_n-;

X₃₃ is -[R₃₂-R₃₃-R₃₁-R₃₂-R₃₂-R₃₁-R₃₂]_n-;

X₃₄ is -[R₃₃-R₃₁-R₃₂-R₃₂-R₃₁-R₃₂-R₃₂]_n-;

X₃₅ is -[R₃₁-R₃₂-R₃₂-R₃₁-R₃₂-R₃₂-R₃₃]_n-;

and

X₃₆ is -[R₃₂-R₃₂-R₃₁-R₃₂-R₃₂-R₃₃-R₃₁]_n-;

X₃₇ is -[R₃₂-R₃₁-R₃₂-R₃₂-R₃₃-R₃₁-R₃₂]_n-, Wherein R₃₁ is a basic hydrophilic amino acid, R₃₂ is a hydrophobic amino acid, R₃₃ is a neutral hydrophilic, basic hydrophilic, or hydrophobic amino acid, and n is from 2 to 5.

17. The composition of Claim 1 wherein X includes the following basic structure X₄₀:

R₃₁-R₃₂-R₃₂-R₃₃-R₃₄-R₃₂-R₃₂-R₃₁-R₃₂-R₃₂-R₃₂-R₃₄-R₃₂-R₃₂,

wherein R₃₁ is a basic hydrophilic amino acid, R₃₂ is a hydrophobic amino acid, R₃₃ is a neutral hydrophilic or hydrophobic amino acid, and R₃₄ is a basic hydrophilic or hydrophobic amino acid.

18. The composition of Claim 1 wherein X includes the following basic structure X₅₀ R₄₁-R₄₁-R₄₂-R₄₁-R₄₂-R₄₂-R₄₁-R₄₁-R₄₂-R₄₁-R₄₁,

wherein R₄₁ is a hydrophobic amino acid, and R₄₂ is a basic hydrophilic or neutral hydrophilic amino acid.

19. The composition of Claim 1 wherein X includes the following basic structure X₅₂:

R₄₁-R₄₁-R₄₂-R₄₂-R₄₁-R₄₁-R₄₂-R₄₂-R₄₁-R₄₂-R₄₂,

wherein R₄₁ is a hydrophobic amino acid, and R₄₂ is a basic hydrophilic or neutral hydrophilic amino acid.

20. The composition of Claim 1 wherein X is a peptide which includes the following basic structure X₆₂:

-R₄₁-R₄₂-R₄₂-R₄₁-R₄₂-R₄₂-R₄₁- wherein R₄₁ is a hydrophobic amino acid, and R₄₂ is a basic hydrophilic or neutral hydrophilic amino acid.

21. The composition of Claim 20 wherein X includes the structure Y₆₂ - X₆₂, wherein X₆₂ is the basic peptide structure of Claim 19, and

Y₆₂ is :

( i) R₄₁- ;

( ii) R₄₂-R₄₁;

( iii) R₄₂-R₄₂-R₄₁; or

( iv) R₄₁-R₄₂-R₄₂-R₄₁.

22. The composition of Claim 20 wherein X includes the structure X₆₂-Z₆₂, wherein X₆₂ is the basic peptide structure of Claim 20, and Z₆₂ is:

( i) R₄₁-;

( i i) R₄₁-R₄₂;

( iii) R₄₁-R₄₂-R₄₂; or

( iv) R₄₁-R₄₂-R₄₂ -R₄₁

23. The composition of Claim 1 wherein X is a basic polypeptide having at least sixteen amino acids, wherein said basic polypeptide includes at least eight hydrophobic amino acids and at least eight hydrophilic amino acids.

24. A method of inhibiting growth of a target cell, virus, or virally-infected cell in a host, comprising:

administering to a host a peptide or protein wherein the N-terminal amino acid is substituted, said peptide or protein having the formula:

W

T - N - X, wherein X is a biologically active peptide or protein, said peptide or protein being an ion channel-forming peptide or protein, N is the nitrogen of the N-terminal amino group, T is a lipophilic moiety, and W is T or hydrogen, wherein said peptide is administered in an amount effective to inhibit growth of a target cell, virus, or virally-infected cell.

25. The method of Claim 24 wherein T is hydrogen.

26. The method of Claim 24 wherein T is: , wherein R is a hydrocarbon having at least 2 and no more than

16 carbon atoms.

27. The method of Claim 26 wherein R is an alkyl group.

28. The method of Claim 27 wherein R is CH₃(CH₂)_n-, wherein n is from 1 to 14.

29. The method of Claim 28 wherein n is from 4 to 9.

30. The method of Claim 29 wherein n is 6.

31. The method of Claim 26 wherein R is: 0 - (CH₂)_z-, wherein z is from 0 to 6.

32. The method of Claim 31 wherein z is 1 or 2.

33. The method of Claim 24 wherein X is a magainin peptide.

34. The method of Claim 24 wherein X is a PGLa peptide.

35. The method of Claim 24 wherein X is an XPF peptide.

36. The method of Claim 24 wherein X is a CPF peptide.

37. The method of Claim 24 wherein X is a cecropin.

38. The method of Claim 24 wherein X is a sarcotoxin.

39. The method of Claim 24 wherein X includes one of the following basic structures X₃₁ through X₃₇, wherein:

X₃₁ is -[R₃₁-R₃₂-R₃₂-R₃₃-R₃₁-R₃₂-R₃₂]_n-;

X₃₂ is -[R₃₂-R₃₂-R₃₃-R₃₁-R₃₂-R₃₂-R₃₁]_n-;

X₃₃ is -[R₃₂-R₃₃-R₃₁-R₃₂-R₃₂-R₃₁-R₃₂]_n- ;

X₃₄ is -[R₃₃-R₃₁-R₃₂-R₃₂-R₃₁-R₃₂-R₃₂]_n-;

X₃₅ is -[R₃₁-R₃₂-R₃₂-R₃1-R₃₂-R₃₂-R₃₃]_n-;

X₃₆ is -[R₃₂-R₃₂-R₃₁-R₃₂-R₃₂-R₃₃-R₃₁]_n-; and

X ₃₇ is -[R₃₂-R₃₁-R₃₂-R₃₂-R₃₃-R₃₁-R₃₂]_n-, wherein R₃₁ is a basic hydrophilic amino acid, R₃₂ is a hydrophobic amino acid, R₃₃ is a neutral hydrophilic, basic hydrophilic, or hydrophobic amino acid, and n is from 2 to 5.

40. The method of Claim 24 wherein X includes the following basic structure X₄₀:

R₃₁-R₃₂-R₃₂-R₃₃-R₃₄-R₃₂-R₃₂-R₃₁-R₃₂-R₃₂-R₃₂-R₃₄-R₃₂-R₃₂,

wherein R₃₁ is a basic hydrophilic amino acid, R₃₂ is a hydrophobic amino acid, R₃₃ is a neutral hydrophilic or hydrophobic amino acid, and R₃₄ is a basic hydrophilic or hydrophobic amino acid.

41. The method of Claim 24 wherein X includes the following basic structure X₅₀:

R₄₁-R₄₂-R₄₂-R₄₁-R₄₂-R₄₂-R₄₁-R₄₁-R₄₂-R₄₁-R₄₁,

wherein R₄₁ is a hydrophobic amino acid, and R₄₂ is a basic hydrophilic or neutral hydrophilic amino acid.

42. The method of Claim 24 wherein X includes the following basic structure X₅₂:

R₄₂-R₄₁-R₄₂-R₄₂-R₄₁-R₄₁-R₄₂-R₄₂-R₄₁-R₄₂-R₄₂,

wherein R₄₁ is a hydrophobic amino acid, and R₄₂ is a basic hydrophilic or neutral hydrophilic amino acid.

43. The method of Claim 24 wherein X is a peptide which includes the following basic structure X₆₂:

-R₄₁-R₄₂-R₄₂-R₄₁-R₄₂-R₄₂-R₄₁- wherein R₄₁ is a hydrophobic amino acid, and R₄₂ is a basic hydrophilic or neutral hydrophilic amino acid.

44. The method of Claim 43 wherein X includes the structure Y₆₂ - X₆₂, wherein X₆₂ is the basic peptide structure of Claim 42, and Y₆₂ is:

( i) R₄₁-;

( 11) R₄₂-R₄₁;

( iii) R₄₁-R₄₂-R₄₁; or

( iv) R₄₁-R₄₂-R₄₂-R₄₁.

45. The method of Claim 43 wherein X includes the structure X₆₂-Z_62, wherein X₆₂ is the basic peptide structure of Claim 44, and Z₆₂ is:

(i) R₄₁-;

(ii) R₄₁-R₄₂;

(iii) R₄₁-R₄₂-R₄₂; or ( iv) R₄₁-R₄₂-R₄₂-R₄₁.

46. The method of Claim 24 wherein X is a basic polypeptide having at least sixteen amino acids, wherein said basic polypeptide includes at least eight hydrophobic amino acids and at least eight hydrophilic amino acids .

47. The composition of Claim 21 wherein X has the structural formula:

(Y₆₂)_a-X₆₂-(Z₆₂)_b, Wherein Z₆₂ is:

(i) R₄₁

(ii) R₄₁-R₄₂;

(iii) R₄₁-R₄₂-R₄₂; or

(iv) R₄₁-R₄₂-R₄₂-R₄₁ a is 0 or 1, and b is 0 or 1.

48. The method of Claim 44 wherein X has the structural formula:

(Y₆₂)_a-X₆₂-(Z₆₂)_b, wherein Z₆₂ is:

( i) R₄₁;

(ii) R₄₁-R₄₂;

(iii) R₄₁-R₄₂-R₄₂; or

(iv) R₄₁-R₄₂-R₄₂-R₄₁, a is 0 or 1, and b is 0 or 1.

49. The method of Claim 24 wherein said peptide is administered to a host in an effective anti-microbial amount.

50. The method of Claim 24 wherein said peptide is administered to a host in an effective anti-viral amount.

51. The method of Claim 24 wherein said peptide is administered in an effective anti-bacterial amount.

52. The method of Claim 24 wherein said peptide is administered in an effective anti-tumor amount.

53. The method of claim 24 wherein said peptide is administered in an effective anti-parasitic amount.