AU2017283194A1 - Light-adjustable hydrogel and bioanalogic intraocular lens - Google Patents

Abstract

A bioanalogic implantable ophthalmic lens ("BIOL") capable of replacing the natural crystalline lens (NCL) in its various essential functions after the NCL having been removed and BIOL implanted into the posterior eye chamber and placed into the capsular bag vacated from the NCL. At least the posterior surface of the lens has a convex shape and is made from a transparent flexible hydrogel material. At least the anterior and posterior optical surfaces are defined by rotation of one or more conic sections along the main optical axis and the surfaces defined by the rotation will include a plane perpendicular to the axis and conical surface symmetrical by the axis. A hydrogel implantable ophthalmic lens whose optical parameters can be optimized and/or customized by a controlled absorption of electromagnetic radiation resulting in a change of the refractive index of the irradiated hydrogel.

Description

LIGHT-ADJUSTABLE HYDROGEL AND BIO ANALOGIC INTRAOCULAR LENS

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims priority to U.S. Application No. 15/190,715, filed June 23, 2016, which is a Continuation-in-Part of U.S. Application Serial No. 14/760,868, filed on July 14, 2015, which is the U.S. National Phase of International Application No. PCT/IB2013/060869, filed on December 12, 2013, which claims the benefit of priority of U.S. Provisional Application No. 61/752,685, filed on January 15, 2013. The entire contents of all of the above applications are incorporated herein by reference.

FIELD OF THE INVENTION

This invention relates to a hydrogel implantable ophthalmic lens whose optical parameters can be optimized and/or customized by a controlled absorption of electromagnetic radiation, such as laser radiation in the visible and/or near-IR region, and more particularly radiation emitted in pulses shorter than one nanosecond (so called Femtosecond Laser, FSL) resulting in a change of the refractive index of the irradiated hydrogel.

BACKGROUND OF THE INVENTION

Intra ocular lenses (IOLs) are surgically implantable lenses which replace or supplement optical function of the NCL. So called “posterior chamber intraocular lens”, or PC IOLs, replace the NCL in the case of cataract or, more recently, in the case of presbyopia by so called “clear lens exchange”, or CLE. Other implantable lenses are placed into the anterior chamber of the eye (AC IOLs), into the cornea (corneal or intrastromal implants) or between the NCL and iris (so called “implantable contact lens” or ICL). So far, most of these IOLs were designed to replace or to supplement the basic optical function of the NCL only. It should be appreciated that an NCL in a human eye, depicted in the Fig. 1, is a complicated structure with several functions. The main eye parts include the cornea 101; the iris 102; the NCL 103; the posterior capsule 104; the cilliary muscle 105; the zonules 106; the vitreous body 107; and the retina 108.

The basic optical function of the NCL 103 consists in helping the cornea 101 to focus the incoming light so that a distant object can be projected on the retina 108. The other important optical function is accommodation - adjustment of optical power of the lens in such a way that objects at various distances can be projected onto the retina 108. There are several theories explaining the accommodation mechanism. See for example L. Werner et al,

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Physiology of Accommodation and Presbyopia, ARQ. BRAS. OFTALMOL. 63(6), DEZEMBRO/2000-503.

The most firmly established theory is von Helmholtz theory explaining that, referring to the Fig. 1, relaxed cilliary muscle 105 causes tension in the zonules 106 that pull the lens 103 periphery outward to keep the NCL 103 in its deformed (flattened) shape that provides a lower refractive power suitable for distant vision. Focusing on a near object is caused by tension in the cilliary muscle 105 that relaxes the zonules 106 and allows the NCL 103 to obtain its “natural” configuration with a smaller diameter, larger central thickness and smaller radii of curvature on both anterior and posterior surfaces. This increases the NCL’s refractive power and allows for projection of the image of near objects on the retina 108.

Most of the common intraocular lenses have spherical surfaces that can be manufactured rather readily. It has been assumed for some time that the NCL 103 is essentially spherical. However, a spherical lens is not exactly monofocal, instead it demonstrates so called “spherical aberration” wherein rays incoming through the center are bent into a focal point that is slightly further from the lens than rays incoming through the lens periphery. Therefore, a spherical lens is somewhat more refractive in its periphery than in its center. This change is continuous: such a lens does not have a single focal point, but many focal points in a short interval of distances (focal range) between the longest and shortest focal distance. In other words, a spherical lens is negatively polyfocal (its focal distance decreases from the center to the periphery). Lenses with elliptical rather than spherical surfaces (such as surfaces created by solidification of a static liquid meniscus) have even more distinct spherical aberration and are, therefore, even more negatively polyfocal than spherical lens.

Some artificial intraocular lenses include hyperbolic surfaces alongside with other surfaces of second order, such as spheric or even elliptic surfaces that have negative polyfocality and very opposite optical effect. More importantly, the prior art generally combines second order (or conic section) surfaces with meniscoid surfaces that are poorly defined and merely approximate second order surfaces with positive spherical aberration (although never surfaces with hyperbolic aberration).

For example, Wichterle in US Pat. No. 4,971,732 claims the meniscoid surfaces to approximate a flat ellipsoid while Stoy in US Pat. No. 5,674,283 considers meniscoid surfaces an approximation of a spherical surface, both having negative polyfocality. A combination of surfaces with positive and negative polyfocality diminishes or negates advantages of the former.

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Furthermore, Wichterle ‘732 describes a manufacturing method of the intraocular lens where a monomer solidifies in an open mold, one (posterior) side of the lens having the shape of the mold cavity while the anterior side has a shape of a solidified liquid meniscus (presumably approximating a flat ellipsoid shape with negative polyfocality, being somewhere between purely spherical and purely ellipsoid surface). The mold cavity has the shape of a second order surface that may include a hyperbolic surface. One can note that each of the optical surfaces is created differently - one by solidification of a polymer precursor against a solid surface while the other by solidification on the liquid-gas interface. It is known to those skilled in the art that the surface quality of the two optical surfaces formed under such different circumstances may differ profoundly in both optical and biological respects.

Wichterle in US Pat. No. 4,846,832 describes another manufacturing method of the intraocular lens where the posterior side of the lens has the shape of the solidified liquid meniscus (presumably approximating a flat ellipsoid shape with negative polyfocality) while the anterior side is formed as an imprint of the solid mold shaped as a second order surface that may implicitly include also a hyperbolic surface. Again, we can note that each of the optical surfaces is created differently - one by solidification of a polymer precursor against a solid surface while the other by solidification on the liquid-gas interface.

Stoy ‘283 discloses modifying the method described by Wichterle ‘732 using a two part mold, one part being similar to the Wichterle’s mold while the other being used to form a modified meniscoid of a smaller diameter on the anterior lens surface. The meniscoid optical surface is of the same character as the meniscoid resulting from Wichterle ‘732, albeit of a smaller diameter and, therefore, probably closer to a spherical surface than an ellipsoid surface. In any case, such a surface has negative polyfocality. The posterior side is formed as an imprint of the solid mold shaped as a second order surface that may include a hyperbolic surface while the other optical surface is formed by solidification of the liquid polymer precursor on the liquid-gas interface.

Michalek and Vacik in PCT/CZ2005/000093 describe an IOL manufacturing method using a spin-casting method in open molds. Molds filled with monomer mixture spin along their vertical axis while polymerization proceeds. One of the optical surfaces is created as the imprint of a solid mold surface while the other is formed by the mold rotation. The imprinted surface has the shape formed by rotation of the conic section along the vertical axis (which may include hyperboloid shape). The other surface is shaped as a meniscoid modified by the centrifugal force that will transfer some of the liquid precursor from the center toward the

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PCT/IB2017/000911 periphery. In the case of the convex meniscus, the centrifugal force will flatten the center and create a steeper curvature in the periphery, i.e. increase the spherical aberration of the surface. In the case of a convex meniscus, the centrifugal force will create a meniscus with smaller central radius and modify the surface to approximate something between spheric and parabolic shape. In any case, the hyperbolic aberration cannot be achieved for either a convex or concave meniscoid surface.

Sulc et al. in US Pat. Nos. 4,994,083 and 4,955,903 discloses an intraocular lens with its anterior face protruding forward in order to be in permanent contact with the iris that will center the lens. Both posterior and anterior surfaces may have the shape obtained by rotation of a conical section around of the optical axis (sphere, parabola, hyperbole, ellipse). The iriscontacting part of the lens is a hydrogel with very high water content (at least 70% and advantageously over 90% of water) that is inherently soft and deformable. Therefore, the optical surface deformed by the contact with iris cannot be exactly a conic section surface, but a surface with a variable shape that will depend on the pupil diameter, probably close to a sphere with a somewhat smaller central radius. Namely, this situation is similar to the lens from another reference that achieves decrease in the central diameter by pressing a deformable gel-filled lens against a pupil-like aperture in an iris-like artificial element (Nun in US Pat. No. 7,220,279). Nun ‘279 does not mention or imply use of hyperboloid optical surfaces. Cummings in US Pat. Publ. Nos. 2007/0129800 and 2008/0269887 discloses a hydraulic accommodating IOL in which a liquid is forced into the internal IOL chamber by action of cilliary apparatus causing thus change of the optical surface and accommodation.

Hong et al. in US Pat. No. 7,350,916 and US Pat. Publ. No. 2006/0244904 disclose an aspheric intraocular lens with at least one optical surface having a spherical aberration in order to compensate the positive spherical aberration of the cornea. The negative spherical aberration is achieved by hyperbolic shape of the optical surface.

Hong et al. in US Pat. Publ. No. 2006/0227286 discloses optimal IOL shape factors for human eyes and defines the optimum lens by a certain range of “shape factors” from -0.5 to +4 (the shape factor being defined by Hong as the ratio of sum of anterior and posterior curvatures to their difference), and at least one of the optical surfaces is advantageously aspherical with conic constant between -76 and -27.

Hong et al. in US Pat. No. 7,350,916 describes an IOL with at least one of the optical surfaces having a negative spherical aberration in a range of about -0.202 microns to about -0.190 microns across the power range.

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SUMMARY OF THE INVENTION

In at least one aspect, the present invention provides an artificial lens implantable into the posterior chamber of human eye for replacement of the natural crystalline lens, the lens (referring to the Fig. 3) having a main optical axis 1A ; the central optical part 2 and the peripheral supporting part 3; the overall shape of the implant being defined by its anterior surface 4, posterior surface 5 and the transition surface 6 between the upper boundaries of the anterior and posterior surfaces 7A and 7B of the implant; having the central anterior optical surface 8A with boundary 9A and anterior apex 10A; the central posterior optical surface 8B with boundary 9B and posterior apex 10B; and anterior peripheral supporting surface 11A and posterior peripheral supporting surface 1 IB.

Artificial lens implantable into the posterior chamber of human eye for replacement of the natural crystalline lens that simulates as closely as practicable the shape, size, optical properties and material properties of an NCL while respecting the need for surgical implantation through a small incision.

The artificial lens according to at least one embodiment of the invention has at least the posterior surface approximating the shape and size of the posterior surface of the natural lens in order to achieve substantially complete contact with the posterior capsule of the eye. As defined in this context, the termsubstantially can mean that either at least about 90% of the posterior BAIOL surface is in contact with the posterior capsule, or at least about 75% of the posterior capsule (that has diameter larger that the lens) is in contact with the posterior surface of the lens. At least the part of the artificial lens according to the invention that is contacting the posterior capsule is made from a transparent flexible hydrogel material approximating the optical, hydrophilic and electrochemical character of tissue forming the natural lens. The anterior side is designed to avoid a permanent contact with iris.

In at least one embodiment, the anterior surface is shaped to avoid a permanent contact with the iris with the anterior peripheral supporting surface 11A being concave. In at least one embodiment, the artificial lens according to the invention has at least the major parts of its anterior and posterior surfaces, including both optical surfaces, defined by rotation of one or more conic sections along the optical axis and formed by solidification of a liquid polymer precursor in contact with a solid wall of a mold, preferably a hydrophobic plastic mold.

One aspect of the invention is directed to a hydrogel comprising UV-absorbing dopant moieties and activator moieties that are negatively charged at physiological pH, where exposure of the fully hydrated hydrogel to electromagnetic radiation results in two-photon

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PCT/IB2017/000911 absorption which causes one or more structural changes in the hydrogel and a change in the refractive index. In one embodiment the hydrogel is a covalently crosslinked hydrogel. In one embodiment, the structural change is achieved without substantially changing the volume of the treated hydrogel segment after it gets into equilibrium with the liquid medium around the lens. One convenient method to determine the volume change can consist in the following procedure: several equal zones in the hydrogel (e.g., 50 x 50 microns) are treated with various laser settings to achieve different phase shift in different zones. After allowing time sufficient to achieve equilibrium, the linear dimension of the treated zones does not change by more than 20% from the original zone dimensions, the dimension change being less than 10% for most conditions. Since the depth of the treated zone cannot be readily or directly measured, it is assumed that the change of the volume is isotropic and the change of treated volume is due to the same relative expansion or contraction for all dimensions. One embodiment of the hydrogel comprises a polymer comprising monomer units of (meth)acrylic acid derivatives and/or (meth)acrylic acid. In one embodiment of the hydrogel the dopant moieties and activator moieties are pendant groups on a polyacrylate or polymethacrylate polymer. In one embodiment the dopant moiety is a UV-absorbing compound that does not strongly absorb light of about 400 nm wavelength. In one embodiment the dopant moiety is a compound selected from the group consisting of rhodamines, benzophenones, coumarins, fluoresceins, benzotriazoles, and derivatives thereof. In one embodiment, the UV absorbent moiety contains a carbonyl group conjugated with an aromatic system, a phenolic hydroxyl group conjugated with an aromatic system, or advantageously both carbonyl and phenolic hydroxyl groups conjugated with an aromatic system. In one embodiment the activator moiety is a compound comprising a carboxylate group, sulfonate group, sulfate group, phenolate group or phosphate group. In one embodiment of the hydrogel the one or more structural changes comprise partial depolymerization of the hydrogel. In one embodiment the partial depolymerization forms an aqueous-filled void in the hydrogel. In one embodiment of the hydrogel the change in the refractive index is a negative change. In one embodiment the depth of the partial depolymerization of the hydrogel depends on the cumulative energy absorbed in a given location of the hydrogel. In one embodiment the hydrogel comprises a polymer comprising monomer units selected from the group consisting of acrylic acid derivatives, methacrylic acid derivatives, acrylic acid, methacrylic acid, and mixtures of two or more thereof.

Another aspect of the invention is directed to an ophthalmic implant comprising the above hydrogel.

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Yet another aspect of the invention is directed to an in .«///-adjustable hydrogel ophthalmic implant comprising an acrylate or methacrylate copolymer hydrogel where the copolymer comprises at least four co-monomers: a) an acrylate or methacrylate ester containing at least one pendant hydroxyl group; b) a polyol acrylate ester or polyol methacrylate ester or amide with at least 2 acrylate or methacrylate groups per polyol ester or amide; c) a derivative of acrylic or methacrylic acid having at least one pendant carboxyl group; and d) a vinyl, acrylic or methacrylic monomer having a pendant UV-absorbing group; where the refractive properties of the implant are adjusted by a controlled absorption of targeted electromagnetic radiation by the hydrogel resulting in a change of refractive index in selected locations of the implant. In one embodiment the ester of component a) carries at least one pendant hydroxyl group on the alcohol portion of the ester. In one embodiment the copolymer is covalently crosslinked. In one embodiment the implant has anterior and posterior refractive surfaces forming a lens with positive or negative refractive power. In one embodiment the lens is an intrastromal lens. In another embodiment the lens is an anterior chamber lens. In yet another embodiment the lens is a phakic lens for location between the iris and the natural crystalline lens. In a further embodiment the lens is a posterior chamber lens for at least partial replacement of the natural crystalline lens. In one embodiment of the hydrogel ophthalmic implant at least one of the refractive surfaces is an aspheric surface with negative spherical aberration. In another embodiment of the hydrogel ophthalmic implant the monomer containing the pendant carboxyl group is a neutralized or partially neutralized methacrylic acid. In one embodiment the monomer containing the pendant carboxyl group is present in a concentration between 0.1 molar % and 5 molar % based on all monomer units of the co-polymer. In another embodiment the monomer containing the pendant carboxyl group is present in a concentration between 0.5 molar % and 2 molar % based on all monomer units of the co-polymer. In one embodiment the monomer containing the pendant UV-absorbing group is present in a concentration between 0.1 molar % and 5 molar % based on all monomer units of the co-polymer. In another embodiment the monomer containing the pendant UV-absorbing group is present in a concentration between 0.2 molar % and 2.5 molar % based on all monomer units of the co-polymer. In one embodiment of the hydrogel ophthalmic implant the pendant UV-absorbing group contains a carbonyl group conjugated to an aromatic group; in one embodiment the monomer containing the pendant UV-absorbing group is present in a concentration between 0.1 molar % and 5 molar % based on all monomer units of the co-polymer. In one embodiment of the hydrogel ophthalmic implant at least one of the UV-absorbing pendant groups is selected from the group consisting of

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PCT/IB2017/000911 derivatives of benzophenone, derivatives of benzotriazole, derivatives of coumarin and derivatives of fluorescein. In one embodiment the pendant carboxyl groups and pendant UVabsorbing groups are present in a molar ratio between about 0.25 and 5; in another embodiment the pendant UV-absorbing groups are present in a molar ratio between about 0.5 and about 3.5. In one embodiment of the hydrogel ophthalmic implant the copolymer contains at least two different comonomers containing different UV-absorbing groups; in one embodiment at least one of the UV-absorbing groups is a benzophenone.

In one embodiment of the ophthalmic lens the pendant carboxyl group is ionized, and the molar ratio of ionized pendant carboxyl groups to UV-absorbing pendant groups is from about 0.5 to about 3.5. In another embodiment of the ophthalmic lens at least the major portion of the polymer of the hydrogel is a derivative of methacrylic acid; in one embodiment at least the major portion of the methacrylic acid derivative is a hydrophilic derivative of methacrylic acid. In one embodiment the hydrophilic methacrylic acid derivative is a glycol ester of methacrylic acid. In one embodiment of the ophthalmic lens the covalently crosslinked hydrogel contains more than 30% by weight of liquid under equilibrium physiological conditions. In one embodiment the covalently crosslinked hydrogel contains less than 55% by weight of liquid under equilibrium physiological conditions. In another embodiment the covalently crosslinked hydrogel contains between 35% and 47.5% by weight of liquid under equilibrium physiological conditions. In one embodiment of the ophthalmic lens at least the posterior optical surface contributes refraction with negative spherical aberration. In another embodiment the posterior optical surface contributes refraction with negative spherical aberration between -0.1 microns and -2 microns. In a further embodiment the negative spherical aberration is between -0.5 microns and -1.5 microns. Alternatively the negative spherical aberration is between -0.75 microns and -1.25 microns. One embodiment of ophthalmic lens comprises both UV-absorbing pendant groups containing carbonyl groups conjugated to an aromatic system as well as UV-absorbing groups containing a benzotriazole structure. In one embodiment the UV-absorbing pendant groups containing carbonyl groups conjugated to an aromatic system and the UV-absorbing groups containing a benzotriazole structure are located in separate layers of the ophthalmic lens. In one embodiment the ophthalmic lens is implanted into a cornea. In another embodiment the lens is implanted into the anterior chamber of an eye between the cornea and iris. In yet another embodiment the lens is a phakic lens implanted between the iris and the natural crystalline lens. In still another embodiment the lens is implanted into the posterior chamber of eye, at least partially replacing the natural crystalline lens.

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Another aspect of the invention is directed to a method of adjusting the refractive properties of a fully hydrated hydrogel of the invention, where the method comprises the step of focused irradiation of the hydrogel with electromagnetic radiation such that two-photon absorption occurs, and the polymer component of the hydrogel undergoes partial depolymerization and/or decomposition, with effective removal of part of the polymer scaffold to create a void.

An additional aspect of the invention is directed to a method of in situ adjusting the optical parameters of a hydrogel ophthalmic implant, the method comprising the steps of: a) providing an eye containing a hydrogel ophthalmic implant according to claim 13; and b) irradiating a portion of the hydrogel ophthalmic implant with electromagnetic radiation using a femtosecond laser, whereby part of the copolymer of the hydrogel is depolymerized and/or ablated; where the optical parameters of the implant are adjusted. In one embodiment of the method the optical parameters include the refractive index. In one embodiment of the method the irradiation produces elongated cavities or voxels inside the hydrogel ophthalmic implant. In some embodiments the voxel depth is up to 20-30 microns or more. In one embodiment increasing the depth of the voxels increases the phase shift while the refractive index remains approximately constant; in one embodiment the refractive index is >1.3335. In some embodiments of the method the phase shift is up to 3 wavelengths of green light. In other embodiments of the method the depolymerized matter ablated by irradiation comprises soluble, easily diffusible compounds of low toxicity. Since the procedure releases only very low concentrations of depolymerized compounds into the intraocular space, the low toxicity of the method is also evident. In one embodiment of the method the modified optical properties are provided by forming in the hydrogel a pattern of elongated voxels of varying depth while keeping the modified refractive index approximately constant. In one embodiment of the method the phase shift is controlled by varying voxel depth rather than by varying their refractive index.

BRIEF DESCRIPTION OF THE DRAWINGS

The accompanying drawings, which are incorporated herein and constitute part of this specification, illustrate the presently preferred embodiments of the invention, and, together with the general description given above and the detailed description given below, serve to explain the features of the invention. In the drawings:

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Fig. 1 illustrates the internal arrangement of the eye with main structures including the cornea, sclera, iris, NCL, vitreous body, retina and the suspensory apparatus of the lens (capsule, zonules and cilliary muscle)

Fig. 2 illustrates distribution of refractive power in a lens with one hyperbolic surface.

Fig. 3 A is a cross-sectional view of a bioanalogic intraocular lens according to an exemplary embodiment of the invention.

Fig. 3B is a top view of the lens of Fig. 3 A.

Fig. 4A is a top view of another exemplary embodiment of a lens with a circular optical part and elliptical support part.

Fig. 4B is a top view of another exemplary embodiment of a lens with a circular support part truncated by a single straight cut.

Fig. 4C is a top view of another exemplary embodiment of a lens with a circular support part truncated by two symmetric crescent cuts.

Fig. 4D is a top view of another exemplary embodiment of a lens with a circular support part truncated by one straight and two crescent cuts.

Fig. 4E is a top view of another exemplary embodiment of a lens with a circular support part truncated by four symmetric crescent cuts.

Fig. 4F is a top view of another exemplary embodiment of a lens with a circular support part truncated by two straight parallel cuts and the cylindrical lens with cylinder axis IB in the angle a with regard to the cuts direction.

Figs. 5 A, 5B and 5C illustrate top views of exemplary lenses with the optical surfaces divided into two or more optical zones.

Figs. 6A, 6B and 6C are cross-sectional views of alterative lens in accordance with the invention composed from two or more materials.

Figs. 7A, 7B and 7C are expanded views illustrating alternative profiles of the supporting peripheral part of the exemplary lenses.

Fig. 8 illustrates the schematic arrangement of the mold for production of a lens in accordance with an exemplary embodiment of the invention.

Fig. 9A shows a comparison of the Raman spectra of a representative hydrogel of the invention before and after two photon absorption (TPA) using a femtosecond laser.

Figs. 9B and 9C show graphs of relevant parameters of the Raman spectrum.

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DETAILED DESCRIPTION OF THE INVENTION

For the purposes of the present application, the terms “(meth)acrylic” and “(meth)acrylate” denotes either acrylic/acrylate or methacrylic/methacrylate moieties. In some embodiments the polymers comprise acrylic/acrylate moieties. In other embodiments the polymers comprise methacrylic/methacrylate moieties. In still other embodiments the polymers comprise both acrylic/acrylate and methacrylic/methacrylate moieties. In a preferred embodiment the polymers comprise methacrylic/methacrylate moieties.

Also, for the purposes of the present application, the term “ablation” refers to removal of a section of the polymeric structural support of a hydrogel, preferably by decomposition of the polymer to diffusible, low-molecular fragments. The term depolymerization constitutes a special type of ablation where the fragments are monomers.

There are numerous types of implantable ophthalmic lenses used in various locations in the eye, from corneal stroma through anterior chamber to posterior chamber. One of the problems with implantable lenses is the complicated selection of the correct refractive properties (so called biometry) and difficulty to replace them or correct them if the biometry turned out to be wrong or if the optical requirements of eye change over time. This inspires the effort of the industry to develop implantable lenses whose optical parameters could be adjusted non-invasively and post-operatively. Change of the optical properties of an artificial lens, either pre-operatively or post-operatively, can be achieved by changing refractive index of the lens material.

The change of the refractive index by action of Two Photon Absorption (TP A) or Multi-Photon Absorption (MPA) for implantable lenses is described in prior art as positive for hydrophilic polymers and negative for hydrophobic polymers. This is consistent with assumed mechanism of increased crosslinking density and consequent decrease of water content in hydrophilic acrylates and hydrogels, and conversely, with increased hydrophilicity in the hydrophobic acrylates. The assumed cause of these changes is a local increase of temperature.

Change of the optical properties of the natural (e.g., human cornea) or an artificial lens by changing refractive index of their material is also described in numerous papers, patents and patent applications, such as the following: Phillips, A.J., System and Method for Treatment of Hyperopia and Myopia, US Patent No. 6,102,906, Bille J.F.: System For Forming And Modifying Lenses And Lenses Formed Thereby, US Patent No. 8,292,952; USP 8,920,690; 9,192,292; Sahler; Ruth et al.: “Hydrophilicity alteration system and

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PCT/IB2017/000911 method” USP 9,023,257; 9,186,242 and 9,107,746; Sahler; Ruth et al.: “intraocular lens (IOL) fabrication system and method” US Patent Application Publication No. 20160074967; Smith, T. et al.: Optical Hydrogel Material With Photosensitizer And Method For Modifying The Refractive Index, US Patent Application Publication Nos. 20130268072; 20090287306 and US Patent No. 8,901,190; Knox, Wayne H. et al.: Optical Material And Method For Modifying The Refractive Index, US Patents Nos. 8,932,352; 8,337,553; 7,789,910 B2; Knox, Wayne H. et al.: Optical Material And Method For Modifying The Refractive Index, US Patent Applications Publication Nos. 20130138093; 20130178934; 20100298933; 20080001320; 20090143858; 20090143858; Knox, Wayne H. et al.: Method For Modifying The Refractive Index Of An Optical Material And Resulting Optical Vision Component, US Patent Application Publication No. 20120310340, Knox, Wayne H. et al.: Method For Modifying Refractive Index Of Ocular Tissues, US Patent Nos. 8,486,055; 8,512,320; 8,617,147 and US Patent Application Publication Nos. 20110071509; 20130226162 and 20140107632; Knox, Wayne H. et al.: Method For Modifying Refractive Index Of Ocular Tissues And Applications Thereof, US Patent Application Publication No. 20120310223, each of which is incorporated herein by reference. None of these prior art references suggests an ablation or depolymerization as the mechanism of the optical adjustment in hydrogels.

Applicant’s co-pending international application, PCT/IB2016/052487, Method and Device for Optimizing Vision Via Customization of Spherical Aberration of Eye, filed on May 02, 2016, contains related disclosure.

The wavelength of the laser beam is usually in the range of near infrared radiation, about 800 nm to 1300 nm, or more typically in the range of visible and near infrared radiation from about 660 nm to about 1100 nm. The use of higher wavelengths is usually preferred because of safety concerns. One method of changing the refractive index of the lens material, by means of a femtosecond laser (“FSL procedure” for short), can, in principle, achieve many changes of refractive properties, such as spherical refractive power, cylindrical refractive power, spherical aberration, etc. In principle, the procedure can selectively change any of the coefficients in the Zemike polynomial (“Zemike Coefficients”) repeatedly even in the implanted lens. This has been demonstrated by Gustavo A. Gandara-Montano et al “Femtosecond laser writing of freeform gradient index microlenses in hydrogel-based contact lenses”, 1 Oct 2015 | Vol. 5, No. 10 | DOI: 10.1364/OME.5.002257 | OPTICAL MATERIALS EXPRESS 2257. The authors noted a negative phase shift in the ETAFILCON® contact lens hydrogel when treated by femtosecond laser at 800 nm. However, none of these systems is in clinical use because, among other reasons, the change

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PCT/IB2017/000911 of the refractive index using an FSL procedure is rather small for the currently available ophthalmic lens materials.

The FSL procedure can be performed both on hydrophilic (where the refractive index

RI is usually increased) and hydrophobic IOL materials (where the RI is usually decreased).

The FSL procedure can be more advantageously performed on hydrogel ophthalmic lenses, particularly on implantable lenses of various types, since the refractive change even in the currently available hydrogels is higher than in the hydrophobic materials. In addition, FSL procedure in hydrophobic acrylates could lead, at least in theory, to so-called “glistening” or other problems related to formation of hydrophilic “osmotic cells” within a hydrophobic material. Therefore, various hydrogels were tested as substrates for micromachining by femtosecond lasers. The sensitivity of hydrogels can be increased by various “dopants” designed to respond to various wavelengths of electromagnetic radiation. A number of dopants have been described so far in the patent and scientific literature. All presently known dopants are single compounds capable of UV absorption, but no known dopants are capable of single-photon absorption at the wavelength used for the FSL procedure.

We have found that the effect of dopants for TPA and MPA in the acrylic and methacrylic hydrogels can be improved, modified and strengthened by adding certain TPA activators, e.g. co-monomers containing negatively charged pendant groups, particularly organic carboxylic acid salts. Both the dopant and its activator can be advantageously covalently bound to polymer chains, more advantageously to the chains forming the polymer network of the hydrogel. Both the dopant and its activator may be bound to the same polymer chain or to different polymer chains.

More particularly, the inventive hydrogels contain a combination dopant in the form of a minor part of a pendant UV-absorbing structure that does not significantly absorb visible light, with a minor part of the activator in the form of pendant groups comprising an ionized salt of an acid, with a major part of methacrylate neutral hydrophilic derivative, particularly glycol or glycerol esters of methacrylic acid and a minor part of a polyol with at least two of the hydroxyl groups esterified by acrylic or methacrylic acid. These hydrogels are materials particularly suitable for adjustment of refractive index by absorption of femtosecond pulses of visible or near infrared radiation (femtosecond laser (FSL) treatment). The absorption of electromagnetic energy causes controlled degradation and depolymerization of the polymeric component of such hydrogels thereby forming domains with lower refractive index. Thanks to the activator, such domains can be formed even if the hydrogel is irradiated by a

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PCT/IB2017/000911 femtosecond laser with pulses of relatively low energy and at a very high “writing speed” or “scanning velocity”.

Disclosed herein are bioanalogic covalently crosslinked acrylic and methacrylic hydrogels containing negatively charged groups, particularly carboxylate groups, and containing also monomers with pendant UV-absorbent groups, such as, for example, methacryloyloxybenzophenone (MOBP). Also disclosed herein is a method to depolymerize such polymers by absorption of electromagnetic radiation in order to adjust optical properties of the implantable hydrogel using femtosecond laser to depolymerize parts of the hydrogel and to form internal cavities that would form new refractive members, such as toric lenses. Implicitly but certainly, such newly formed cavities in the hydrogel matrix would be necessarily filled with water or aqueous fluid (either alone or also containing the residual of the degraded polymer component of the hydrogel) and would have, therefore, different (specifically lower) refractive index than the parent hydrogel material. Also disclosed is the possibility to perform this lens adjustment in situ on the implant since the products of such a decomposition are water-soluble compounds of low toxicity and capable of a slow diffusion through the hydrogel.

We have confirmed that the UV-absorbent group used in the hydrogel acts as a “dopant” increasing the depolymerization rate, while the negatively charged group acts as an “activator” for the dopant, increasing the dopant’s efficacy still further. In addition, the activator acting as a quencher protects the material from undesirable “charring” or “burning” that may happen over a certain amount of absorbed energy per time. This allows one to lower the overall amount of energy needed to achieve a certain refractive change, and therefore to use safely the more energetic visible light rather than less energetic near infrared (NIR) radiation.

Without wishing to limit the scope of the invention by any particular hypothesis or theory, the proposed explanation of the phenomena of the hydrogel depolymerization by electromagnetic radiation is as follows. Focused femtosecond lasers are known to facilitate two-photon-absorption (TPA) (or even Multi-Photon Absorption (MPA) in their focal volume. The volume of the material affected by the TPA is usually called “volume pixel”, or “voxel”. The size of the voxel depends on various parameters, and generally increases with the absorbed energy. The smallest possible voxel size corresponds to the “focal volume”, or an ellipsoid with volume roughly the cube of the wavelength of the laser light absorbed. The diameter of a voxel is much smaller than its depth thanks to the self-focusing of the laser beam in the case of TPA or MPA, and is typically around 500 nm. The voxel depth is much

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PCT/IB2017/000911 larger and grows with deposited energy. The reported maximum voxel depth is about 6 microns in currently known materials.

If the radiation of a certain wavelength is absorbed via TPA or MPA, then the dopant accumulates excitation energy equivalent to a single-photon absorption of the wavelength of incident light divided by the number of the absorbed photons. For example, laser light at 400 nm absorbed via TPA by the dopant will correspond to 200 nm light absorbed via SPA. Light of 200 nm wavelength is a hard UV light (UVC Band) that is sufficiently energetic to cause a breakage of chemical bonds and a deep structural rearrangement. Of course, 3 photon absorption or 4 photon absorption, although much less probable than TPA, would accumulate even higher energy concentration. The excited state with high accumulated energy is shortlived since the energy dissipates quickly via one of several possible pathways, the most usual being conversion into thermal energy. The usual effect of femtosecond laser treatment of tissues or synthetic hydrogels involves local increase of temperature that may cause depending on the amount of the energy absorbed - effects starting with conversion of the matter into a plasma (e.g., via laser ablation) through heating sufficient for charring, to more subtle additional crosslinking via various mechanisms such as re-esterification, disproportionation or dehydration with formation of ether links.

However, the presence of the activator (pendant negatively charged groups, particularly carboxylate groups), changes the mechanism of this process. We believe that this is caused by certain cooperation of the activator groups with dopants. In this cooperation, activation of dopants further facilitates TPA absorption and increases its effect. The activator, such as the carboxylic acid group of methacrylic acid or a salt thereof, is apparently in an interaction with the dopant because its presence causes a change of the dopant’s UV/Vis spectrum (namely, a subtle shift from the UV toward the visible region). This may increase the dopant’s “TPA cross-section” and increase the absorption efficacy of the dopant.

However, the main role of the activator may be in channeling the absorbed energy of the two (or more for MPA) absorbed photons from the dopant to the main polymer chain to cause breakage of the covalent bond in the main chain. It is understood from the general character of this process, and from the known depolymerization kinetics of methacrylate polymers, that this cleavage is homopolar and produces free radicals that start the depolymerization process in the vicinity of the negatively charged activator (e.g., carboxylate). This free radical mechanism may vastly increase the quantum yield of the photodegradation and helps to explain why even a relatively low concentration (on molar

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PCT/IB2017/000911 basis) of the dopant can cause profound changes in the local composition and structure, and consequently a substantial change in the local refractive index.

Furthermore, the activator groups help to divert the absorbed energy from the dopant to quench the excited state and “consume” this energy for the breakage of covalent bonds. This “energy conduit” function of the activator prevents excessive heat accumulation in the vicinity of the dopant moiety, helping thus to “recycle” the dopant molecule and preserve it for the future TPA cycle. It also helps to protect the polymer structure from burning and charring and helps to increase the efficacy of the whole absorption process.

In addition, this proposed mechanism can explain the fact that in the absence of the activator, TPA in hydrogels with only the dopant proceeds via a different mechanism and achieves the opposite result: while in presence of the activator the refractive index decreases, in its absence the refractive index increases. One possible reason for the increased refractive index seems to be an additional crosslinking leading to decreased water content and, consequently, increased refractive index since water has the lowest refractive index of all hydrogel constituents.

Another consequence of the stipulated mechanism involving the activator is the reduced (or even eliminated) change of the volume due the TPA process. Namely, in the case of the additional crosslinking the water content is reduced while the amount of the polymer components stays approximately the same. Therefore, the mass and the volume of the treated hydrogel volume has to be reduced, with various adverse consequences on the product (generation of internal stress, change of geometry and change of mechanical properties, for instance). Conversely, if the polymer becomes more hydrophilic and attracts more water, the treated volume of the polymer has to expand (again, with adverse effect on the geometry, optics and stresses within the material).

The mechanism stipulated in the present invention involves exchange of a certain part of the polymer mass for water, with a low net volume change, if any. The depolymerization of the hydrogels covered by the present invention yields low toxicity monomers and/or their fragments, primarily 2-hydroxyethyl methacrylate, methacrylic acid and ethylene glycol. All these decomposition products are well soluble in water and capable of diffusing through the surrounding intact hydrogel network, to be dissipated from the implant over some time in very low concentrations.

The proposed mechanism may explain very large phase shift achievable in hydrogels according to this invention. The phase shift is determined by the change in the refractive index and the length of light path through material with changed refractive index, in other

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PCT/IB2017/000911 words, the voxel depth. Refractive index in hydrogels cannot be practically lower than refractive index of water, or about 1.3335 since the lowest conceivable index can be achieved in a cavity filled with aqueous liquid. The cavity in a hydrophilic polymer matrix cannot be filled with a gas for certain basic thermodynamic reasons, and there is no known or readily conceivable mechanism of creating a hydrophobic cavity within the hydrogel.

Therefore, large phase shifts (more than one wavelength) will require formation of voxels with large depth. It is usually assumed that the voxel depths can reach at most about 5 to 6 microns. However, if the refractive index in the focal volume decreases, then the elongated voxel would act as a light-guide that channels additional light pulses to be absorbed in the voxel bottom. Consequently, the depth of the voxel can gradually increase with increasing number of pulses, and so does then the phase shift even though the refractive index remains approximately constant and equal to or higher than 1.3335. This mechanism is then different from mechanisms described to date where voxel size remains approximately constant but refractive index change increases with increased absorbed energy (achieved e.g., number of pulses). It is believed that the presently proposed mechanism may provide a voxel depth larger than 10 microns, and phase shifts corresponding to voxel depth as high as 25 or 30 microns have been observed. This is mentioned not to set a limit on the invention, but demonstrate the fundamental difference of the invention from prior art. One of the practical consequences of this difference is the following: materials used in the prior art allow for creation of refractive or diffractive structures by forming a refractive index gradient (GRIN) while hydrogels according to the present invention allow achieving similar refractive or diffractive effects by forming a pattern of varying voxel depth while keeping the modified refractive index approximately constant. In addition, it is believed that this refractive index value first approaches and ultimately approximates the refractive index of isotonic saline solution.

As a consequence of this novel mechanism, the newly created refractive or diffractive structures form a system of parallel waveguides (i.e., elongated voxels with refractive index lower than the surrounding hydrogel) of different length, whereby the phase shift is controlled by the varying voxel depth rather than their varying refractive index.

An additional feature of the invention is creation of a gradient of refractive index in the vicinity of individual voxels. The monomers and other fragments released by the depolymerization inside the voxel migrate radially by diffusion through the surrounding hydrogel. When the temperature decreases below the ceiling temperature (around 200°C in the case of methacrylates), at least part of the released monomers and/or fragments may re17

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PCT/IB2017/000911 polymerize and create a denser network structure with a higher refractive index than the parent hydrogel. This mechanism has two beneficial consequences: first, it reduces the amount of compounds that diffuse outside of the implant to be metabolized, and second, the gradient of refractive index thus formed improves the light-guiding properties of the voxel.

Thus, monomers released by depolymerization may not all diffuse out of the hydrogel, but may partly re-polymerize in the voxel vicinity. In that sense the present method is somewhat similar to FS laser ablation in that that both remove some polymer mass and retain water instead, although the term “ablation” frequently denotes a process of decomposition where the polymer and water are converted into a plasma (gas). The presently disclosed process is much more gentle so that polymer decomposition does not create an exploding bubble of gas.

Although depolymerization of the hydrogel copolymer is believed to be the primary mechanism involved in the presently disclosed process, it may be supplemented by other decomposition reactions that form small, water-soluble fragments, such as hydrolysis of the (meth)acrylate pendant groups, or oxidation.

Dopant concentration in hydrogels according to the invention varies from about 0.05 %-mol to about 5%-mol, advantageously from about 0.1 %-mol to about 2.5 %-mol. Alternatively the different optimum dopant concentration can be used for various dopants, e,g, 0.25 to 0.55 molar % for benzophenone derivatives, or 0.1 to 0.2 molar % for benzotriazole derivatives. The preferred dopants are UV absorbers with low absorbance for visible light, i.e. above a wavelength of about 390 nm. Examples of suitable dopants are vinyl, acrylate or methacrylate derivatives of benzophenone, benzotriazole and coumarin, although those skilled in the art can certainly identify other suitable UV absorbers that work as dopants in the sense of the invention.

The activating groups are present in concentration from about 0.25 %-mol to about 5 %-mol, advantageously from about 0.75 to about 3.5 %-mol. In some embodiments the preferred molar concentration of methacrylic acid is between 1 and 1.25 % molar. The preferred activator groups are derivatives of acrylic or methacrylic acid containing a pendant acidic group including carboxylate group, sulphate group, sulfonate group and phosphate group. Such acidic groups are preferably neutralized by suitable organic or inorganic cations. The activator to dopant group molar ratio should be about 0.75 to 10, advantageously from about 1 to about 5 . Alternatively the different optimum molar ratio activator/dopant are different for different dopant. For instance, the optimum ratio for benzophenone derivatives is from about 2 and 4 and for benzotriazol derivatives between about 5 and about 7.

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The preferred composition of a hydrogel according to the invention comprises a major fraction of a methacrylate monomer with pendant neutral hydrophilic groups. By “major fraction” is meant at least 50%-mol of all monomer units in the hydrogel. In some embodiments the molar fraction of the hydrophilic methacrylate monomer is between 90%mol and 99.5%-mol, and in many cases between 97.5%-mol and 99%-mol. Such hydrophilic methacrylate monomers can be esters of polyol aliphatic compounds, such as glycols, glycol ethers, glycerol and sugars. The most familiar in this group is 2-hydroxyethylmethacrylate (2-HEMA). Alternative monomers to the above mentioned esters comprise amides of methacrylic acid, such as methacrylamide, N-isopropyl methacrylamide or N-(2hydroxyethyl) methacrylamide. This major fraction of the hydrogel polymer may also comprise a mixture of such hydrophilic monomers. Alternatively, a minor portion of the methacrylate monomers (but not more than 25%-mol) can be replaced by analogous acrylic acid derivatives. In some embodiments 0.5 %-mol to 5%-mol can be replaced by acrylate monomers.

A minor part of the monomer units is formed by the above mentioned activator monomers with a pendant negatively charged group, and still another minor part is formed by the above mentioned dopant monomers.

The polymer is advantageously covalently crosslinked. The crosslinking can be achieved by any of the methods known to those skilled in the art, such as radiation crosslinking, crosslinking by formation of ether links between pendant OH groups, etc.The preferred crosslinking method is copolymerization with a minor fraction of methacrylate or acrylate crosslinking diesters or triesters of polyols, such as, for example, triethylenglycol dimethacrylate.

Refractive index of the hydrogel according to the invention is between about 1.38 and about 1.48, preferably between about 1.40 and about 1.45. In some embodiments the RI is about 1.40, or about 1.41, or about 1.42, or about 1.43, or about 1.44, or about 1.45.

The preferred hydrogel according to the invention contains between about 25%-wt and about 85%-wt liquid in equilibrium with live intraocular environment. For intraocular lenses supplementing or replacing the natural crystalline lens, the more advantageous equilibrium liquid concentration is between 35 %-wt and 50 %-wt, and more particularly between 40% and 47%-wt. In some embodiments the equilibrium water content is 41%-wt ±0.75%, or 42.5 ±l%-wt, or 44.5 ±1% by weight. It is understood by those skilled in the art that equilibrium liquid content in hydrogels is subject to many variables, such as body

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PCT/IB2017/000911 temperature, composition of body fluids or pressure exerted by surrounding tissues or body structures. Also, measurement of liquid content in small hydrogel implants may be burdened by a certain measurement error, so that these values are illustrative. Since the negatively charged activator groups tend to increase equilibrium liquid content and thus to decrease the refractive index of the hydrogel, the higher concentration of the activator may be compensated with addition of hydrophobic acrylates or methacrylates, such as methylmethacrylate, ethylmethacrylate, benzylmethacrylate, isobomylmethacrylate or ethoxyethyl methacrylate that will reduce the water content and increase refractive index into a desirable range for the ophthalmic implant design. Typical concentration of such added methacrylates or acrylates is up to about 40% molar. In some embodiments the concentration of added hydrophobic monomers is between about 5%-molar and 25%-molar.

The dopant group and activating group may be located on the same polymer chain, or on different polymer chains that are in an intimate contact e.g. in a polymer blend, or being on two different segments of polymer network. One or other may be on a graft, or one can be on a graft and the other on the basic chain. The dopant group and activating group may be on a single molecule in a suitable steric relationship allowing their mutual interaction. Such a “dopant-activator complex” may be then covalently bound to a polymer chain, or admixed into the polymer, or become part of the main polymer chain.

Negatively charged activator groups have some additional advantages for intraocular implants, such as improved biocompatibility, resistance to adsorption of proteins, resistance to formation of biofilms, resistance to calcification, and resistance to adhesion and spreading of cells (which translates into resistance to sclerotization of posterior capsule and resistance to posterior capsule opacification).

The hydrogel implant according to the invention can be placed in various locations within the eye along the optical path. It may have the form of a “blank” in which a refractive or diffractive lens is created, or it may have the form of a refractive or diffractive lens which optical properties are modified by the refractive index change within selected locations of the implant.

Intraocular lenses may replace partly or fully the natural crystalline lens. Intraocular hydrogel lenses have some advantages over other IOL types, and various types and related manufacturing methods are described, e..g., in the following patents and patent applications: Stoy, V. et al.: Bioanalogic Intraocular Lens, International Patent Application WO2014111769; Wichterle, 0.: Method Of Molding An Intraocular Lens, US Patent No. 4846832; Wichterle, 0.: Soft And Elastic Intracameral Lens And Method For Manufacturing

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Thereof, US Patent No. 4,846,832; Stoy, V.: Implantable Ophthalmic Lens, A Method Of Manufacturing Same And A Mold For Carrying Out Said Method, US Patent No. 5,674,283; Sulc, J., et al.: Soft Intracameral Lens, US Patent Nos. 4,994,083 and US Patent 4,955,903; and Michalek, J. et al.: Method Of Manufacturing An Implantable Intraocular Planar/Convex, Biconvex, Planar/Concave Or Convex/Concave Lens And A Lens Made Using This Method, US Patent No. 8409481; each of which is incorporated herein by reference.

Another type of implantable ophthalmic lens is so called “Implantable Contact Lens” (or ICL). ICL is a phakic lens placed between iris and the natural crystalline lens. It is described in several patents, e.g., Fedorov , et al. Intraocular lens for correcting moderate to severe hypermetropia, US 5,766,245; Feingold V., Intraocular contact lens and method of implantation, UP 5,913,898; Intraocular refractive correction lens, US 6,106,553; each of which is incorporated herein by reference.

These implantable lenses are made from hydrogels that incorporate a biological component, usually collagen. These so called “Collamers” are described in various patents, such as Feingold, et al., Biocompatible, optically transparent, ultraviolet light absorbing, polymeric material based upon collagen and method of making, US 5,910,537; Feingold, et al. Biocompatible optically transparent polymeric material based upon collagen and method of making, US 5,654,349, US 5,654,388 and US 5,661,218; Fedorov, et al., Biocompatible polymeric materials, methods of preparing such materials and uses thereof, US 5,993,796; Method of preparing a biological material for use in ophthalmology; each of which is incorporated herein by reference.

Another approach to refractive correction is intrastromal or intracorneal implants or inlays, described e.g. by Miller in Aspherical corneal implant, US 7,776,086; Lang, Alan in Design of Inlays With Intrinsic Diopter Power, US 2007/0255401; Dishler; Jon et al. in Small Diameter Inlays, US 2007/0203577; Lang, Alan et al. in Intracorneal Inlays, US 2007/0129797; and Dishler, et al. in Method of using small diameter intracorneal inlays to treat visual impairment, US 8,057,541; each of which is incorporated herein by reference.

Some intrastromal implants are designed for post-operative adjustment of optical power by laser, as described by Peyman, Gholam A. in Intrastromal corneal modification via laser, US 2001/0027314; in Adjustable ablatable inlay, US 2002/0138069 and 2002/0138070; Ablatable intracorneal inlay with predetermined refractive properties in US 2003/0093066; and in Bifocal implant and method for altering the refractive properties of the eye, US 2005/0222679; each of which is incorporated herein by reference.

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There are also intraocular implants using two lenses in tandem in order to achieve improved accommodation capability, modularity of the design or reduced size of implantation incision when the implant is in situ assembled from individual parts.

All such ophthalmic lens types can be manufactured from the hydrogels according to the present disclosure and then modified by using a femtosecond laser treatment.

Again, without wishing to be limited by any particular theory, the partial depolymerization of the presently disclosed method appears to be the mechanism of the profound negative phase shift observed in the inventive hydrogels when exposed to focused femtosecond lasers (FSLs). As noted above, this process appears to generate a system of parallel longitudinal voxels that get more elongated with increasing number of absorbed FSP pulses. The voxel length controls the phase shift achievable for a given refractive index, that cannot decrease below the RI value for water, which is 1.3335. Further, it is actually the phase shift, not the refractive index per se, that is affected in the presently disclosed hydrogels and methods. This mechanism is different from anything disclosed by the prior art (presumed crosslinking leading to decrease in the water content that leads to the refractive index increase and, therefore, a corresponding positive phase shift).

A further difference from the prior art is that the presently disclosed hydrogels and methods replace part of the hydrogel-forming copolymer with water or aqueous fluid, rather than decreasing the water content of the hydrogel by modifying the copolymer properties (such as by polymer crosslinking).

In the drawings, like numerals indicate like elements throughout. Certain terminology is used herein for convenience only and is not to be taken as a limitation on the present invention. The following describes preferred embodiments of the present invention. However, it should be understood, based on this disclosure, that the invention is not limited by the preferred embodiments described herein.

The NCL has a very complicated structure that develops over time. One of the structural features is asphericity of posterior and anterior surfaces of the NCL 103. As established in recent years E.L.Markwell et al, MRI study of the change in crystalline lens shape with accommodation and aging in humans, Journal of Vision (20110 11(3);19, 1-16; M.Dubbelman et al, Change in shape of the aging human crystalline lens with accommodation, Vision Research 45 (2005), 117-132;F. Manns et al, Radius of curvature and asphericity of the anterior and posterior surface of human cadaver crystalline lens, Experimental Eye Research 78 (2004), 39-51;M. Dubbelman et al, The shape of the aging human lens: curvature, equivalent refractive index and the lens paradox, Vision Research 41

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PCT/IB2017/000911 (2001) 1867-1877, both anterior and posterior surfaces of a young human lens are hyperbolic and can be characterized by the equation:

Y - Yo = X^A2/{ Ro*(l+l-h*(X/Ro)^A2)^A0.5} equation 1 where Y is the coordinate in the direction of the main optical axis 1 A, X is the distance from the main optical axis 1 A, Yo is the apex position on the main optical axis 1 A, Ro is the central radius of curvature and h is the conic constant (or the shape parameter). The Eq. 1 describes any conic section curve depending on the shape parameter h value: it is a parabola for h= 0, a circle for h = 1, hyperbole for h<0, prolate ellipse for 0<h<l and oblate ellipse for h > 1.

It has been found that for a typical young human NCL, the anterior surface is more hyperbolic than the posterior surface, that hyperbolicity increases significantly with accommodation, and that the human lens grows with age and its hyperbolicity decreases so that an old NCL may become approximately spherical.

The referenced studies mapped dimensions of typical NCL for selected population samples. According to these references, a typical human lens anterior central radius ranges from about 5 to 13 mm and the average anterior conic parameter is about -4 (ranging from about -22 to +6). The posterior central radius ranges from about 4 to 8 mm and the average posterior conic parameter is about -3 (ranging from about -14 to +3).

The central thickness of a young, relaxed NCL ranges typically from about 3.2 mm to about 4.2 mm, increasing with age and/or with the near-focus adjustment to a thickness from about 3.5 mm to about 5.4 mm. The posterior part depth of the NCL is typically the same as, or larger than the anterior part depth. Therefore, the sagittal depth of the posterior lens surface is typically from about 1.75 mm to about 2.75 mm on equatorial diameter from about 8.4 mm to about 10 mm. This defines the basic dimensions of the posterior capsule in its “natural” state.

Although the above references do not state any particular connection between the geometry and optical properties of the NCL, we have found by mathematical modeling that the hyperbolic surfaces turn a lens polyfocal, with the refractive power maximum at its center and gradually decreasing toward the periphery. One direct consequence expected from such a polyfocality is a large focal depth of the lens so that a near object can be projected on the retina even without any particular lens shape change. Another implication of the modeling is that the average refractive power of the lens increases with decreasing aperture. Therefore, it is concluded that the near focus can be improved by pupil constriction (this so called “pupillary reflex” or “near myosis” that can be actually clinically observed at near focus).

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Another consequence of the natural lens hyperbolicity is the capability of the human (and particularly young) brain to naturally neuro-adapt to, and correctly interpret images formed by projection through a hyperbolic lens onto retina.

This accommodative mechanism utilizing certain type of the polyfocality deserves further explanation as follows.

Lenses with at least one hyperbolic surface demonstrate a “hyperbolic aberration” that is opposite of the spherical aberration: rays incoming through the center are bent into a focal point that is closest to the lens, and the focal point becomes progressively further from the lens for rays incoming in increasing distance from the lens center toward the lens periphery.

Therefore, the lens with hyperbolic surface is positively polyfocal: it has the shortest focal distance (i.e., highest refractive power) at its center, and the focal distance increases (i.e., refractive power decreases) from the center toward the lens periphery. The focal range of a hyperbolic lens can be rather large and is controllable by so called conic constant or shape parameter defining the hyperbolic surface shape.

Examples of the distribution of refractive power in a lens with hyperbolic surface is shown in Fig. 2 where local refractive power in Diopters m'¹ are plotted against the distance from the optical axis in mm. It is understood that such an optical profile with refractive power decreasing with the distance from the optical axis, or with the aperture of the imaging system, or pupil diameter of the eye, can be present in the originally implanted lens, or it may be created by the hydrogel modification by a laser after lens implantation.

Based on the present studies it is understood that the positive polyfocality and its changes in the natural lens assist the eye to accommodate in several ways:

It projects on the retina simultaneously images of all objects in the field of view in all distances covered by the focal range of the lens. This significantly (by more than 1 Diopter) increases the depth of the focus of the eye since all objects create a well-focused image (accompanied by many dis-focused images that the brain learns to suppress).

The natural lens increases its hyperbolicity due to the accommodation, which further increases the focal range of the lens and, therefore, the depth of the focus still further.

The eye helps to focus on near objects by narrowing the pupil. This so called “pupillary reflex” or “near myosis” has two consequences: first, it decreases the aperture and thus increases depth of the focus of the eye as the optical system (narrowing aperture blocks rays that are far from the axis and coming in at sharp angles with respect to the axis); and it increases the average refractive power of the lens by using only its central portion with the highest refractive power.

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It is obvious from our studies that near myosis can assist the near focus only for lenses with hyperbolic aberration, i.e. with positive polyfocality. It has little effect in monofocal parabolic lenses, and it is counterproductive in lenses with negative polyfocality: spherical or elliptical (e.g., meniscoid) lens becomes weaker lens with lower refractive power by the near myosis rather than a stronger lens that is needed for near focus.

An artificial lens according to the invention is a hydrogel device implantable into the posterior chamber of human eye for replacement of the natural crystalline lens. It is designed to mimic or replicate essential physiological and optical functions of natural lens without creating problems that earlier attempts could cause in some situations. It is important to recognize that this is achieved by a novel thoughtful combination of features that might have been individually, or in different combinations, applied previously with a lesser success. The natural lens also achieves its function due to its balanced combination of features rather than to a single feature.

The features contributing to the overall function and combined according to the invention include size and shape of the implant; material properties; surface properties; optical properties; implantation method; and manufacturing method. We will describe the various features below and provide exemplary configurations of how individual features mutually interact to provide beneficial effect. It is important to recognize that the implant may combine several of the described features to achieve desirable effects, however, the invention is not limited to the exemplary configurations described below and includes various combinations of features.

Referring to Figs. 3 A and 3B, the implant has a main optical axis 1A with a central optical part 2 and a peripheral supporting part 3. The overall shape of the implant is defined by its anterior surface 4, posterior surface 5 and the transition surface 6 between the upper boundaries 7A and 7B of the anterior and posterior faces, respectively. Each face is composed of two or more surfaces. The anterior central optical surface 8A has boundary 9A and central posterior optical surface 8B has boundary 9B. Each of the surfaces may be divided into two or more zones with the boundary between them (denoted 13 A and 13B in Figs. 5 A to 5C) being circles, straight lines or otherwise defined shapes. The apexes of the central anterior optical surface 10A and central posterior optical surface 10B are positioned on the main optical axis 1 A. The anterior peripheral supporting surface is 11A and the posterior peripheral supporting surface is 1 IB.

Referring to the Figures 6A, 6B and 6C describing lenses comprising several different materials or layers, it is understood that any layer or structure in the optical path may be

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PCT/IB2017/000911 formed by the hydrogel according to the present invention. The preferred layer may be the layer closest to the cornea, i.e. the one forming the anterior optical surface of the lens. Optical structures formed by the hydrogel optical modification may be refractive structures, diffractive structures, Fresnel lenses shown in the Fig. 6B, refractive index gradient lenses or similarly. Such structures can be formed within the hydrogel, or on its surface, preferably on the anterior optical surface.

The boundaries 7A and 7B are distinguishable as a discontinuity on the top of the anterior and posterior surfaces 4 and 5, respectively. Such a discontinuity lay in the inflexion point of the surface in the direction of the optical axis, or a in a point of discontinuity of the second derivative of the surface in the direction of the optical axis. The boundary can be rounded and continuous, but advantageously it is formed by a sharp rim or edge. The advantage of the sharp edge is in forming the obstacle to migration of cells such as fibroblasts along the capsule surface (the usual reason for posterior capsule opacification).

The overall lens diameter is defined as the larger diameter of the boundaries 7A and 7B. The lens optical zone diameter is defined as the smallest diameter of the boundaries 9A and 9B. The posterior sagittal depth is the vertical distance between the posterior apex 10B and the plane defining the posterior boundary 7B. Central thickness is the distance between apexes 10A and 10B. Anterior depth is the vertical distance between the anterior apex 10A and the plane defining the anterior boundary 7A.

The main optical axis 1A may be the axis of symmetry in the case that boundaries 7A and 7B, as well as boundaries 9A and 9B, are defined by circles in the plane perpendicular to the optical axis, and if the central optical part 2 is symmetrical and e.g., does not have any cylindrical component. Such implant with symmetric circular footprint is shown in Fig. 3B. However, the rims and boundaries may have other than circular footprint, e.g. elliptical as shown in Fig. 4A, or may have the footprint shaped as a truncated circle in Figs. 4B to 4E with single, double, triple or quadruple truncating cuts 12A to 12D. These truncated footprint shapes serve several purposes:

They provide better access into the space behind the lens during the implantation. It is important to clean this space well in order to remove any viscoelastic polymers or lubricants or other auxiliary agents before the surgical incision is closed.

They prevent rotation of the lens after the capsule shrinks around the IOL. This is particularly important for toric lenses.

They facilitate folding and insertion through a small incision.

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In the case that the optics has a cylindrical component, then the cylinder axis IB will be positioned in a defined way with respect to the asymmetry of the outside rim, e.g. be in the angle a to the truncating cuts 12A and 12B as shown in the Fig. 4F. Needless to say that the truncating cuts 12A to 12D may not be necessarily straight cuts, but may be suitably formed to e.g. a crescent shape, and their number may be even higher than 4. Also, the truncating cuts may not be of the same length or positioned symmetrically. It can be appreciated that the footprint with truncated rim will facilitate folding of the implant and its insertion through a small surgical incision. In addition, the asymmetric rim footprint will prevent the implant rotation once the capsule settles around it. This is particularly important for tone lenses with a cylindrical component designed to compensate for astigmatism.

The posterior surface 5 is shaped and sized to approximate the shape and size of the posterior surface of the natural lens and to achieve contact with at least the major part of the posterior capsule of the eye. This is important for several reasons:

The implant will keep the posterior capsule in its natural shape, unwrinkled and smooth for the optimum optical performance;

The tight contact between the capsule and the implant will prevent migration of fibroblasts that could cause the posterior capsule opacification; this is particularly effective if the posterior surface is highly hydrated and carrying fixed negative charge.

The implant will occupy the space vacated by the posterior side of the natural lens and keep thus vitreous body from advancing forward and prevent thus the decrease of the pressure of vitreous body against retina (which could facilitate retinal detachment and/or cystoic macular edema).

It should be noted that the intimate contact between the implant and posterior capsule is beneficial particularly if the contacting surface of the implant is hydrophilic and carrying fixed negative charge in order to prevent capsular fibrosis and its consequent stiffening, opacification and contraction that would interfere with the implant function (or could even dislocate it), as will be described hereinafter.

In the preferred embodiment of the invention, at least the major part of the posterior surface 5 is formed by a generally smooth convex surface formed by rotation of conic sections around the optical axis, or a combination of such surfaces. The peripheral part is preferably formed by a conic surface or a hyperboloid surface, while the central optical surface is preferably hyperboloid, paraboloid or spherical surface (or a combination thereof). The sagittal depth of the posterior surface (i.e. the vertical distance between the posterior central optical surface apex 10B and the boundary of the posterior surface 7B, measured on

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PCT/IB2017/000911 the main optical axis IA) should be larger than 1.1 mm in order for lens to perform its function well. To perform well in the whole refractive range, the posterior sagittal depth should be larger than 1.25 mm, advantageously larger than 1.75 mm and preferably larger than 2 mm, but in any case less than about 2.75 mm.

The overall outer diameter of the implant (LOD) is important for its centricity, position stability and capsule-filling capability. The outer diameter of the posterior surface 5,

i.e. the largest dimension of the posterior outer boundary 7B (in the plane perpendicular to the main axis 1 A) should be larger than 8.4 mm, desirably at least 8.9 mm and preferably at least 9.2 mm. The largest outer diameter permissible is about 11 mm, but desirably should be lower than 10.75 mm and preferably at smaller than 10.5 mm. The considerable flexibility in the outer dimensions is allowed by several factors - flexibility of the lens, and particularly flexibility of the outer peripheral supporting part 3 that can accommodate various capsule sizes and capsule contraction without deforming the central optical part 2.

The central optical surfaces may consist of one or more zones with different geometry. The zones may be concentric, in which case the posterior boundary 13B between them in the Fig. 5 A will be circular. Zones may also be divided by straight boundaries, in which case the zones may have crescent or wedge footprint. Various examples are shown in Figs. 5A to 5C. The zones may be on the anterior or posterior optical surface. Fig. 5A shows the posterior optical surface is divided by the boundary 13B into two concentric optical zones - the central optical zone 8B1 and the outer optical zone 8B2. For instance, the posterior optical surface of the central optical zone 8B1 may be a spherical or parabolic zone used for the sharp near vision, while the hyperbolic outer zone serves for intermediate and far distance vision. Alternatively, both zones may have hyperboloid surfaces with different central radii Ro and/or different conic constants. Each optical surface may be also divided into more than two zones. The example in Fig. 5B shows the top view of the lens which anterior optical surface 8 A is divided by a straight boundary 13 A into two optical zones of equal area 8 Al and 8A2. Each of those zones has different shape with different optical parameters. The example in Fig. 5C shows a top view of a lens with anterior optical surface 8A divided by two straight boundaries 13 A and 13B into four paired optical zones 8Al and 8A2, each having a different area and different optical parameters. For instance, 8A1 may have higher refractive power that 8A2 and serve for near focus. One of the zones may have a cylindrical component.

Both optical surfaces (or their zones or segments) are surfaces formed by rotation of a conical section along the optical axis, or by a combination thereof. One or both optical

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PCT/IB2017/000911 surfaces may contain one or more spherical optical zones. Advantageously, at least one of the optical surfaces comprises at least one hyperbolic surface, preferably in the outer optical zone. Preferably, both optical surfaces comprise at least one hyperbolic zone each. Such hyperbolic surface resembles the surfaces of the NCL and mimics some of its beneficial optical properties. Even more preferably, both posterior and anterior optical surfaces are hyperbolic surfaces or a combination of two or more concentric hyperbolic zones. Lenses with at least one hyperbolic surface have so called hyperbolic aberration, the very opposite of spherical aberration of lenses with spherical, ellipsoid or meniscoid surfaces. The lenses with hyperbolic aberration have highest refraction in the center and gradually decreasing with distance from the optical axis. (In lenses with spherical aberration the refractive power increases with distance from the optical axis.) The hyperbolic aberration helps the eye to accommodate through several mechanisms described above. It should be understood that hyperbolic aberration can be created not only by the exactly hyperbolic surfaces in the geometry sense, but also by similar surfaces where surface steepness generally decreases with the distance from the optical axis. Therefore, by “hyperbolic surfaces” are meant also other hyperbole-like surfaces approximating this property.

As the spherical aberration is measured with increasing aperture (or pupil diameter) the negative spherical aberration of hyperboloid-like surfaces increases in absolute value (i.e., gets more negative). The spherical aberration can be expressed in various alternative ways, such as a deviation of wave-front in microns, or as steepness of decrease of local refractive power from the optical axis, or by decrease of the refractive power with increasing aperture, or by the value of conic constant or shape parameter corresponding to such optical profiles. Those skilled in the art can readily convert one of such values into the corresponding value expressed in a different way. The implant according to the present invention can have originally any spherical aberration, since its value can be adjusted post-operatively using the method of this invention.

The spherical aberration in the final implanted state (i.e., after the hydrogel modification by a laser) will be generally between about -0.1 microns and -2 microns on aperture 4.5 mm. Preferably, the final spherical aberration will be between about -0.5 microns and -1.5 microns on aperture 4.5 mm, and even more advantageously the spherical aberration will be between about -0.75 microns and -1.25 microns on the aperture 4.5 mm.

In order to mimic the optical properties of the NCL, conical constants of the anterior and posterior optical surfaces are selected so that the refractive power of the central optical

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PCT/IB2017/000911 part 2 generally decreases from the highest value at the optical axis to the lowest value at the periphery of the central optical part 2.

The steepness of the refractive power decrease with the distance from optical axis is dependent on the shape parameter (conic constant) of the hyperbolic surface. The conic parameter should be selected that the average decrease of the refractive power is between 0.25 Dpt/mm and -3 Dpt/mm, advantageously between -0.5 Dpt/mm and -2.5 Dpt/mm and preferably between about -1 Dpt/mm and -2 Dpt/mm.

The posterior central radius of curvature (at the point where the optical axis intersects the posterior apex) is advantageously from 2.5 to 8 mm, and preferably from about 3.0 to 5 mm. The conic constant of the posterior surface is advantageously selected from the range of about +3 to about -14 reported for NCL, preferably from about -1 to -8.

The central radius Ro of the anterior optical surface 8A is selected to be either larger than about +3 mm or smaller than about -3 mm, and preferably larger than from about +5 mm or smaller than about -5 mm.

The conical constant of the anterior optical surface 8A is selected from the range from +6 to -22 reported from human NCL, preferably from the range between about -1 to -8 mm.

The anterior optical surface 8A may be formed partly or fully by a spherical surface or a parabolic surface. In that case the central posterior optical surface 8B should be preferably hyperbolic with the conic parameter selected in such a range so that the whole lens has hyperbolic aberration.

Preferably though, at least the major part of the anterior optical surface 8 A is a hyperboloid surface, particularly the outer optical zone. The central optical zone of the anterior optical surface having diameter between about 1.5 to 4 mm, advantageously between about 2 and 3.5 mm, can be formed by parabolic or spherical surface in order to further improve the near focus resolution.

Fig. 2 shows schematically one example of the preferred optical profile of the lens according to the invention. It should be appreciated that different eyes require different refractive power of the implanted lens.

Most of the current IOLs are not bioanalogic since they are designed to simulate just the basic optical function of NCL, i.e. to provide the basic refractive power needed to focus a distant object on retina. Depending on the specific eye, the basic refractive power is usually between 15 and 30 Dpt, with some deviations on either side. This requirement can be met by an approximately monofocal (usually spherical) rigid lens located somewhere near of the principal plane of the NCL. Since most detailed images are projected onto a relatively small

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PCT/IB2017/000911 part of retina (macula) located on the optical axis, and since many of our activities are performed at small eye aperture (constricted pupil), most IOLs are significantly smaller than the NCL (4.5 to 6 mm for most IOLs as opposed to 9.5 to 10.5 mm for the NCL). The small size of optics is preferred by some IOL manufacturers for easier adaptation of such IOL for implantation through a small incision. For the same reason, most IOLs are made from a soft, elastic material that allows implantation through a small incision in a deformed (folded, rolled, etc.) shape. This deformability has no relation to the optical function, however.

Small size of optics has its disadvantages, however. IOL edges may reflect light at large pupil opening (e.g., during night driving) and cause glare, halos and other adverse effects. Besides, a small optic cannot project all peripheral and off-axis rays that NCL does, particularly at a large pupil opening. Lastly, a small size optics interferes with clear visibility of retinal periphery that is sometimes needed for diagnostics and treatment. For those reasons, the large optics similar in size to NCL is preferable over a smaller one that is used in most of the current IOLs. Importantly, the whole large optical zone has to have well defined geometry to be optically useful. Lenses with meniscoid optical surfaces have poorly defined shape particularly in the peripheral region. This may cause unexpected and disturbing optical phenomena.

Some modern IOLs are designed to simulate to some extent the accommodation or pseudoaccomodation of the NCL (i.e. allowing the eye to focus on both far and near objects). Various IOLs use different means to achieve this goal: some are using bifocal, multifocal or polyfocal optics; others are using designs allowing anterior-posterior shift of the IOL optics with respect to the eye; or allow change of optical power by changing mutual position between two lenses. Some lenses even change the refractive power due to liquid transfer within the lens driven by pressure of cilliary muscles and/or vitreous body, change of head position or by a miniature pump.

These designs are sometimes rather intricate contraptions, very different in size, shape and material properties from the NCL. This makes them susceptible to various problems, such as fibrosis of the capsule or cell ingrowth or protein deposits on their surfaces that interfere with their function. In addition, their increased bulk and complicated design interferes with the need of all modem IOLs to be implantable through a small incision. This requires designs with small-diameter optics and use of materials with high refractive index that are more reflective than the NCL, increasing thus the glare and halo problems.

In most cases, these lenses are using optics of a small diameter, typically 4.5 to 6 mm, with slender, flexible “haptics” to position the optics in the center of the optical path. In

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PCT/IB2017/000911 addition, deformable materials are used to allow folding or rolling for implantation through a small incision. The surface properties of such IOLs are sometimes modified to achieve better biocompatibility (e.g., A. M Domschke in the US Pat. Publ. No. 2012/0147323, J. Salamone et al in the US Pat. Publ. No. 2008/0003259).

This common design allows folding the IOL for the implantation through a relatively small incision (usually 2 to 3 mm). However, the small IOL size has its own drawbacks:

The small optics with diameter 6 mm or less may not fully replace the crystalline lens of diameter 9 to 10.5 mm if eye aperture is large due to poor light conditions (causing night glare, halos, limited peripheral vision etc.) or if the IOL becomes decentered (causing the “sunset syndrome” or other problems);

Small optics cannot project all peripheral and off-axis rays the NCL does, reducing thus the imaging performance particularly at large pupil openings (needed for e.g., night peripheral vision);

Small optics may complicate or even prevent retinal examination and treatment (which may be important particularly in the case of diabetics).

In addition, a small IOL size leaves essentially vacant the space that was originally occupied by the much larger NCL. Consequently, the vitreous body is allowed to advance and its pressure against the retina is partly relieved. This may cause an increased probability of retinal detachment after the cataract surgery as reported by J. A. Rowe, J.C. Erie, K.H. Baratz et al. (1999). “Retinal detachment in Olmsted County, Minnesota, 1976 through 1995”. Ophthalmology 106 (1): 154-159. The same effect may also cause or facilitate Cystoid Macular Edema (CME). See Steven R. Virata, The Retina Center, Lafayette, Indiana: Cystoid Macular Edema, WEB page.

There is another disadvantage of a small optics and the conventional IOL design with haptics: The IOL with optics suspended in the relatively vacant space by means of relatively fragile haptics may be sensitive to damage and/or dislocation in case of an accidental impact (fall on a slippery surface, car collision, a punch, etc.).

Some problems derived from a small bulk of IOLs and small-diameter optics are being addressed by IOL designs that fill the space vacated by NCL to a smaller or larger extent. There are several approaches to this, each with its own advantages and disadvantages:

Capsule-filling by a liquid that can solidify into a clear, flexible solid such as a silicone rubber. As long as the filler material has similar deformability as the NCL, it was expected that this approach would restore the natural lens accommodation (e.g., Gasser et al. in US Pat. No. 5,224,957). However, the materials used so far often cause fibrosis and

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PCT/IB2017/000911 opacification of the capsule. Besides, it is difficult to control the shape and optical parameters of the in situ formed IOL

Implantation of a large, bulky IOL in a highly deformed shape that allow implantation through a reasonably small incision and fills a significant part of the capsule. This approach was tried with hydrophobic memory polymers that can be “frozen” in a highly deformed shape for implantation, and returns into the original functional shape upon heating to body temperature (Gupta in US Pat. No. 4,834,750 and US Pat. No. RE 36,150). However, the hydrophobic memory polymer is very foreign material and causes similar problems like the materials used to fill the capsule.

Similar approach was also tried with hydrogels. Very large IOLs, mimicking size and shape of the natural lens, have been implanted into the vacated capsule (e.g., Wichterle ‘732 and Stoy ‘283). The problem of these particular IOLs was their peculiar optics. These lenses had meniscoid anterior optical surfaces that deviated strongly from the geometry of an NCL. The meniscoid shape was formed by solidification of a free surface of the monomer mixture, and there was a problem with control of the optical properties of such IOLs. In addition, these lenses were often too bulky for implantation through a small incision. Moreover, some of the hydrogels used in these lenses lacked the fixed negative charge, and such hydrogels have tendency to calcify sometime after their implantation. Some other capsule-filling lenses (Sulc et al. ‘083 and ‘903) had anterior protrusions touching the iris and stabilizing thus the lens in the approximately central position but causing various problems such as blockage of the liquid flow, deformation of lens optics and iris erosion.

Another approach was implantation of a hollow lens (or a lens shell) that was filled after implantation by a liquid solidifying in situ (e.g., Nakada, et al. in US Pat. Nos. 5,091,121 and 5,035,710).

Another approach was implantation of dual-optics IOLs with two lenses, one being in contact with anterior and the other with the posterior capsule, both lenses being kept apart by flexible members or connectors (US Pat. No. 4,946,469; US Pat. No. 4,963,148; US Pat. No. 5,275,623; US Pat. No. 6,423,094; US Pat. No. 6,488,708; US Pat. No. 6,761,737; US Pat. No. 6,764,511; US Pat. No. 6,767,363; US Pat. No. 6,786,934; US Pat. No. 6,818,158; US Pat. No. 6,846,326; US Pat. No. 6,858,040; US Pat. No. 6,884,261).

Such implants filling essentially the whole capsule of the original crystalline lens have also some problems:

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Unless made from extremely biocompatible materials with similar hydration and negative charge as NCL, the anterior face of the IOL may touch the iris and cause its erosion, depigmentation, bleeding or inflammation.

Some materials are made more biocompatible by having high equilibrium water content. However, that decreases their refractive index far below the optimum value (the value for young NCL).

Important for the IOL is not only the shape and optics type, but also its material. An NCL is composed of an intricate natural hydrogel structure comprising water, salts, and polymeric component containing collagenous proteins, polysaccharides and proteoglycans. Importantly, the polymeric components contain a considerable concentration of acidic ionizable groups, such as carboxylates or sulfates. These groups provide the lens material with a fixed negative charge. The hydration and the negative charge influence the interaction between the NCL and proteins in the intraocular fluids. Furthermore, its surface properties affect the interaction between the lens and cells. It is known that synthetic hydrogels containing surface with a fixed negative charge do not attract the proteins and cells and make hydrogel more resistant to calcification (Karel Smetana Jr. et al, “Intraocular biocompatibility of Hydroxy ethylmethacrylate and Methacrylic Acid Copolymer/ Partially Hydrolyzed Poly(2-Hydroxyethyl Methacrylate),” Journal of Biomedical Materials Research (1987) vol. 21 pp. 1247-1253), and are not recognized as a foreign body by immune system (Karel Smetana Jr. et al, “The Influence of Hydrogel Functional Groups on Cell Behavior”, Journal of Biomedical Materials Research (1990) vol. 24 pp. 463-470). Although many IOL manufacturers avoid materials with carboxylate groups based on the assumption that carboxylates attract calcium ions and thereby cause calcifications, there are several references to hydrogel IOLs containing carboxylate groups (Wichterle ‘732, Sulc et al. ‘083 and ‘903, Stoy in in US Pat. No. 5,939,208, Michalek and Vacik in ‘093).

Carboxylate groups may be uniformly dispersed in the hydrogel, or concentrated mainly on the surface forming a gradient of swelling and charge density, as described e.g. in Stoy ‘208 and Sulc et al. US Pat. No. 5,158,832. Typically, the NCL material contains, on average, about 66% by weight of water. However, the NCL is structured with denser core and more hydrated jacket and the NCL hydration changes with age and from individual to individual. Therefore, one cannot assign a single water content value to the NCL other than average.

Similarly, various layers of the NCL have different refractive indices. The refractive index of the lens varies from approximately 1.406 in the central layers down to 1.386 in less

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PCT/IB2017/000911 dense layers of the lens. See e.g. Hecht, Eugene. Optics, 2nd ed. (1987), Addison Wesley, ISBN 0-201-11609-X. p. 178. Therefore, the optically meaningful equivalent refractive index, or ERI, is given as the characteristic of the NCL. Both refractive index and water content change with the lens age. Average ERI = 1.441 -3.9xlO^A-4xAGE, decreasing thus from about 1.441 at birth to about 1.414 at 70 years. See M. Dubbelman et al. “The Shape Of The Aging Human Lens: Curvature, Equivalent Refractive Index And The Lens Paradox”, Vision Research 41 (2001) 1867-1877, FIG 9.

In addition, the ERI increases with accommodation by about 0.0013 - 0.0015 per Diopter. See M.Dubbelman et al, “Change In Shape Of The Aging Human Crystalline Lens With Accommodation”, Vision Research 45 (2005), 117-132Ref pp. 127-128. One can speculate that this change of refractive index is related to a change (decrease) of water content due to the lens deformation during the accommodation. Disregarding these complications, we will use the average ERI = 1.42 unless stated differently.

Interesting to see that it is very difficult, if not impossible to find a synthetic hydrogel with same water content and - at the same time - refractive index as the NCL material. Specifically, a synthetic hydrogel containing 66% by wt of water would typically have a refractive index of about 1.395 rather than 1.42 that would be expected with hydrogel containing closer to 50% of water.

The average liquid contents for ERI=1.441 (very young average NCL) would be 40% of water while for ERI = 1.414 (old average NCL) would need a hydrogel with water content about 55% by weight. Since we believe that for bioanalogic IOL material it is more important to simulate refractive index than water content of NCL, we have selected the desirable average water content range of the IOL according to an exemplary embodiment of the invention, to be between 40% and 55% by weight. Of course, this is the average water content - similarly as with the NCL, the lens may have various layers with different water contents, e.g. inner parts with higher refractive index and outer layers with lower refractive index.

A number of prior art references mention lOLs from hydrogels with high water content, however, they do not recognize the relation between the water content and the refractive index value. For instance, Wichterle ‘732 specifies the desirable refractive index value around 1.4 (broadly from 1.37 to 1.45, which is clearly impossible for known synthetic hydrogels with the specified water content: at least 60% and preferably 65 to 70% translates into the refractive index range from 1.39 to 1.405). The examples show formulations with a low content of carboxylate groups.

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Sulc et al. ‘083 and ‘903 disclose water content at least 70% and advantageously at least 90% on the surface or its part, and mentions 55-70% water content in prior art IOLs. A core with higher and a casing with lower refractive index are mentioned, and the core may have the form of a Fresnel lens. The gradient of both hydration and refractive index is optionally obtained by NaOH treatment that achieves reorganization of the hydrogel covalent network. Example 1 of this reference shows an IOL with water content 88.5 %, Example 2 shows the IOL with water content 81% , and Example 4 shows the lens with water content 91%. No water content is given for Example 3.

Charles Freeman in US Pat. Publ. No. 2009/0023835 describes a hydrogel material with water content lower than 55% and refractive index higher than 1.41 and the sodium ion flux in the range of about 16 to about 20 micro.eq-mm/hr/cm², useful particularly for phakic posterior chamber IOLs. No carboxyl or acidic groups are mentioned, although their presence is known to increase the ion diffusion flux through the hydrogel.

Hydrogel character of the NCL material has some possible, less obvious but potentially important consequences: its water content is dependent on the pressure against the lens. Consequently, the NCL adjusted to the far distance may have a different water content, and therefore a different refractive index, than the relaxed lens adjusted to the near objects. Since the stress in the NCL adjusted for far distance is not distributed evenly, a gradient of swelling and gradient of refractive index may result. This will create subtle changes in the optical properties, in addition to the polyfocality of the NCL surfaces. These subtle changes may be important for vision, and it will be difficult to replicate them otherwise rather than by using a hydrogel of similar physical-chemical and optical properties, as well as geometry similar to that of an NCL. In particular, the hydrogel of the NCL substitute should have a similar refractive index and capability to change water content by an external stress that can be reasonably expected to act on an NCL. Therefore, the hydrogel used in a bioanalogic IOL should have a hydraulic flow capability for water.

Therefore, at least the part of the implant contacting the posterior capsule is made from a transparent flexible hydrogel material approximating the optical, hydrophilic and electrochemical character of tissue forming the natural lens.

The anterior part of the IOL may interfere with, or even block the flow of the vitreous humor causing thus increase of IOP and ultimately glaucoma. This design often requires a preemptive iridectomy.

Unless made from extremely biocompatible materials with similar hydration and negative charge as an NCL, the large-area contact between the capsule and artificial materials

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PCT/IB2017/000911 used in current IOLs sometimes cause the capsule opacifications, fibrosis, etc.. These problems are now being solved by the bioanalogic intraocular lens according to this invention.

The central optical part 2 is made of a deformable, elastic material, such as a hydrogel with equilibrium water content between about 35 and 65%, advantageously between about 38% and 55% and preferably between about 40% and 50% (all % are weight percent and equilibrium water content is water content in equilibrium with intraocular fluid, unless stated otherwise).

Deformability of the optical part is important both for the implantation through a small incision and for its accommodation function. The optical part may be constructed as a hydrogel shell with a core composed from a liquid or a soft gel, as shown in the Fig. 6A. Fig. 6A shows a cross-sectional view of a lens with the posterior hydrogel jacket 14, the softer core 15 and the anterior shell 16. The posterior hydrogel jacket 14 is advantageously integral with the peripheral supporting part 3 of the lens and contains the fixed negative charge at least on its posterior surface. The core 15 can be advantageously made from a hydrophobic liquid, such as mineral oil or silicone oil, or from a soft silicone or acrylic slightly cross linked gel that can be easily designed and created by those skilled in the art. Alternatively, the core can be made or a hydrophilic fluid or a soft hydrogel. The anterior shell 16 can be made from the same or different material as the posterior hydrogel jacket 14.

In one embodiment, the hydrogel jacket and the soft core 15 have essentially the same refractive index so that the major part of the refraction takes place on the outer optical surfaces of the lens rather than on its internal interfaces. This can be achieved e.g. by making the core from a silicone liquid or a silicone gel having refractive index around 1.42, and making the jacket from a hydrogel with water content between about 41 and 45% of water. By formulating the hydrogel correctly one skilled in the art can adjust the water content in the hydrogel to achieve the substantial match of the refractive indices. Alternatively, the core and the jacket can have different refractive indices so that part of the refraction takes place on the internal interfaces between materials.

Fig. 6B a cross-sectional view of a lens with an internal interface between the core 15 and adjacent optical medium 16 that is shaped to form a compound lens, e.g. a Fresnel lens. The materials of core 15 and the optical medium 16 have different refractive indices, and one of them is advantageously a fluid that can improve both deformability and refraction. The zone 15 or 16 (the one with the lower refractive index) can be created by the modification of a hydrogel according to the present invention using a laser. The hydrogel modification can be

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PCT/IB2017/000911 carried out either preoperatively or postoperatively. Advantage of this arrangement is the possibility to use hydrogels with high water content and low refractive index as the basic construction material, and yet achieve relatively low central thickness of the lens that allows implantation through a small incision.

Fig. 6C shows an alternative design of the lens comprising two different materials. Material on the posterior side 14 is a hydrogel with high hydration rate and containing negatively charged groups. It is the same for the optical and supporting part. The anterior side material of core 15 is a material with lower water content and higher refractive index. The interface between the two materials is refractive.

Both central optical anterior surface 8A and central posterior optical surface 8B have a diameter larger than about 5.6 mm, advantageously larger than about 6.5 mm and preferably larger than about 7.2 mm. Optimum diameter of the larger of the two optical surfaces is larger than about 7.5 mm, advantageously about 8 mm to approximate the size of the NCL optics. Such a large optic is usually suitable for convex-concave or plano-convex central optical part 2. For a biconvex optical part, the anterior optical diameter is usually selected smaller in order to minimize the central thickness of the optical part. In any case, the diameter of the anterior optical surface 8A is advantageously not larger than the diameter of the central posterior optical surface 8B.

The central optical surfaces 8A and 8B are surrounded by boundaries 9A and 9B that are not necessarily circular. The boundaries 9A and/or 9B may be also elliptical or have a shape of a truncated circle, in order to facilitate the lens folding and implantation through a small incision. Non-circular optical surfaces are particularly suitable for lenses with a cylindrical component.

The posterior peripheral supporting surface 1 IB is formed by a convex surface, advantageously a hyperbolic or conical surface with the axis identical with the main optical axis 1 A. This surface is highly hydrophilic and carrying a fixed negative charge due to a content of acidic groups such as carboxylate, sulfo, sulphate or phosphate groups. This combination of hydration and negative charge prevents a permanent adhesion to the capsule, prevents migration of cells, particularly fibroblasts, along the interface between the lens and the capsule, decreases irreversible protein adsorption, and discourages capsular fibrosis and opacification. The posterior peripheral surface is advantageously limited by a sharp edge 7B that further discourages cell migration toward the optical zone.

The anterior peripheral supporting surface 11A is a concave surface with its apex located on the optical axis and it is preferably symmetrical along the axis 1 A.

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Advantageously it is a conical or hyperbolic surface with its axis coinciding with the main optical axis 1 A. The surface is advantageously highly hydrophilic and carrying fixed negative charge in order to discourage cell adhesion and migration and anterior capsular fibrosis. The anterior peripheral surface is advantageously limited by a sharp edge 7A that further discourages cell migration.

The anterior and posterior peripheral supporting surfaces 11A and 1 IB together with the connecting surface 6 define the shape of the peripheral supporting part 3. The peripheral supporting part is convex on the posterior side and concave on the anterior side, the average distance between the two surfaces ranging from about 0.05 to 1 mm, advantageously from about 0.1 to 0.6 mm and preferably from about 0.15 to 0.35 mm. The optimum distance depends on the stiffness of the material that is dependent on water content, negative charge density, crosslinking density and other parameters.

If the posterior and anterior surfaces are formed by surfaces of similar geometry, such as hyperbolic surfaces, then the peripheral supporting part 3 will have even thickness. The arrangement shown in Fig. 7A has the advantage to be readily deformable and adjustable to various sizes of the capsule, and two sharp edges 7A and 7B preventing migration of fibroblasts toward the optical zone.

The peripheral supporting part 3 can be also made less or more deformable by increasing or decreasing its thickness from the rim toward the center, as shown in Figs. 7B and 7C, respectively. These figures also show various alternative arrangements of edges 7A and 7B.

The anterior surface 4 of the implant is shaped to avoid any permanent contact with iris that could cause iris erosion, pupilar block, iris pigment transfer to the implant and other problems. Such a contact could also interfere with the flow of the intraocular fluid causing thus adverse changes of the intraocular pressure. It could also interfere with the contraction of the pupil as to prevent so called near myosis that helps the near focus both by the natural lens and by the implant according to the invention. Therefore, the anterior central optical surface 8A part is partially sunk due to the anterior peripheral supporting surface 11A concavity and due to positioning the boundary 9A under the plane defined by the anterior boundary 7A. The central anterior surface 8A is a plane, a convex surface or a concave surface with its anterior apex 10A not exceeding the uppermost point of the lens (the higher of 7 A and 7B) by more than about 0.25 mm, advantageously not exceeding the upper rim at all and preferably having the anterior apex 10A bellow the uppermost point 7A by at least 0.1 mm.

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At least the major part (including the central optical surfaces 8 A and 8B) of both anterior and posterior surfaces 4 and 5 are defined by rotation of one or more conic sections around the main optical axis 1 A. wherein the term “conic section” includes a segment of a line for purpose of this application. The surfaces defined by the rotation will include a plane perpendicular to the axis and conical surface symmetrical by the main optical axis 1 A. The peripheral supporting part is convex on the posterior side and concave on the anterior side, the average distance between the two surfaces ranging from about 0.05 to 1 mm, advantageously from about 0.1 to 0.6 mm and preferably from about 0.15 to 0.35 mm.

In at least one embodiment, the lens according to the invention is manufactured by solidification of liquid polymer precursors. In the preferred embodiment, the solidification takes place in contact with a solid mold, particularly a mold made of a hydrophobic plastic. It can be appreciated that the surface microstructure of a polymer depends on the environment in which its solidification took place. The surface microstructure will be different if the solidification occurs on the solid liquid interface that if it takes place on the liquid-liquid or liquid-gas interface. Preferably, at least all optical surfaces are created by solidification of the precursor on a solid interface. Even more preferably, whole surface of the implant is formed by solidification of a liquid precursor against a solid surface, particularly a hydrophobic plastic surface. Preferred plastic for the mold is a polyolefin, and particularly preferred plastic is polypropylene. The polyolefin has low polarity and has low interaction with highly polar monomers that are used as hydrogel precursors. Likewise, the hydrogel formed by the liquid precursor solidification has very low adhesion to the mold surface and can be cleanly detached without even a microscopic surface damage. This is important for both optical properties and for long-term biocompatibility of the implant.

Manufacturing a relatively large lens of a precise shape by molding is difficult. It is recognized by those skilled in the art that any solidification of the liquid precursor is accompanied by the volume shrinkage that may even exceed 20 percent. In a closed mold of a constant volume, such a shrinkage will prevent copying of the internal mold surface and cause formation of vacuoles, bubbles, surface deformities and other imperfections. This is the main reason why the meniscus casting methods described above were used for IOL molding. Other inventors have described a method and a mold design allowing the excess of monomers to be transported from adjacent spaces by the suction created by the volume contraction (Shepherd T., US Pat. No. 4,815,690). However, this method cannot be used in cases where the liquid precursor gellifies at a low conversion (e.g., 5 to 10 percent) due to the crosslinking polymerization.

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We have discovered a different method for the volume shrinkage compensation, namely, decrease of the internal mold cavity volume due to the deformation of certain mold parts. The mold depicted in Fig. 8 is composed from two parts 18A and 18B, the part 18A being used for molding the anterior surface 4 and the part 18B for molding the posterior surface 5.

The shaping surface 19B of the part 18B has a shape needed to form the posterior optical surface 8B of the lens. The peripheral part 22B of the molding surface has a diameter larger than the diameter of the lens and advantageously a hyperboloid or conical shape The part 18A has the shaping surface 19A that is divided into the central part 21A shaping the anterior optical surface 8A of the lens, and the peripheral part 22A of the diameter larger than the diameter of the lens. The peripheral part 22A has advantageously a hyperboloid or conical shape. The peripheral surface 22A is substantially parallel to the corresponding surface 22B of the part 18B.

The diameter of the molding the mold parts 18A and 18B are larger than diameter of the lens and advantageously they are the same. One of the surfaces for 22A or 22B is equipped with a relatively thin and deformable barrier 20 with inner surface corresponding to the geometry of the surface 6 of the lens. The height of the part 20 is typically between about 0.05 mm and 1.3 mm, and its thickness is lesser than its height. The profile of the part 20 is advantageously wedge-like or triangular. At least one of its surfaces is advantageously parallel to the optical axis IA. The barrier 20 may be separate from the parts 18A and 18B, but advantageously it is an integral part of one of them. Advantageously, this part 20 is located on the concave surface 22B. In a preferred mode of the operation, the liquid precursor is filled into the concave mold part 18B in a slight excess to reach over the barrier 20, and then it is covered with the part 18A. The mold is constructed in such a way that the only contact between parts 18A and 18B is via the part 20. The solidification of the precursor generates its contraction and the consequent decrease of the pressure in the mold cavity. At a low conversion, the additional liquid precursor is pulled into the mold cavity. Once the gelpoint is reached due to the crosslinking, the precursor cannot flow anymore. The decreased pressure will cause deformation of the part 20 and decrease of the distance between parts 18A and 18B and the consequent decrease of the molding cavity volume. The two-part mold for the IOL according to the invention is preferably made by injection molding from a polyolefin, advantageously from polypropylene.

The preferred liquid precursor for the invention is a mixture of acrylic and/or methacrylic monomers with crosslinkers, initiators and other components known well to

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PCT/IB2017/000911 those skilled in the art. The preferred precursor composition comprises a mixture of acrylic and/or methacrylic monoesters and diesters of glycols where monoesters are hydrophilic components and diesters are crosslinkers. The preferred precursor also comprises acrylic and/or methacrylic acid or its salts. It advantageously comprises also a UV absorbing molecule with a polymerizable double bond, such as methacryloyloxybenzophenone (MOBP). Other possible derivatives of acrylic or methacrylic acid are their esters, amides, amidines and salts.

Also part of the hydrogel structure are ionizable groups bearing a negative charge, such as carboxylate, sulfate, phosphate or sulfonate pendant groups. They may be introduced by copolymerization with appropriate monomers bearing such groups, such as methacrylic or acrylic acid. In this case, the ionogenic functionality will be uniformly dispersed in the hydrogel. Particularly advantageous are hydrogels with ionogenic groups concentrated mainly on the surface with the consequent gradient of swelling and charge density. Such gradients can be created by after-treatment of molded lenses, e.g. by methods described in Stoy ‘208 and Sulc et al. US Pat. Nos. 5,080683 and 5,158,832.

Other methods include, e.g. grafting of monomers comprising ionogenic groups on the lens surface. It is understood that only a part of the lens surface may be treated to contain high concentration of ionogenic groups, or that different parts of the surface may be treated by different methods.

The lens according to the invention can be implanted in the deformed and partly dehydrated state. The controlled partial dehydration can be achieved by contacting lens with a suitably hypertonic aqueous solution of physiologically acceptable salts, such as chlorides, sulfates or phosphates magnesium or monovalent ions, such as sodium or potassium. Salt concentration can be adjusted to achieve hydration between about 15% and 25% by weight of the liquid. The lens in the hypertonic solution can be advantageously sterilized by autoclaving.

Another method for preparing the hydrogel lens for implantation through an incision with reduced size is plastification of the hydrogel by a non-toxic organic water-miscible solvent, such as glycerol or dimethylsulfoxide, in such a way that the plasticized hydrogel has softening temperature above ambient but lower than eye temperature. Such composition and process is described e.g. in Sulc et al, US Pat. No. 4,834,753 that is hereby incorporated by this reference.

The lens according to at least one embodiment of the invention is advantageously implanted in the state of the osmotic non-equilibrium to adhere to the tissue temporarily. The

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PCT/IB2017/000911 osmotic non-equilibrium allows the lens centering by adhering it against the posterior capsule while the capsule shrinks around it. Once the lens is enveloped by the capsule, its position is stabilized. The osmotic non-equilibrium can be achieved in various ways: soaking the lens prior to the implantation in a hypertonic salt solution, e.g. in a solution of 10% to 22% by wt. NaCl, advantageously 15% to 19% by wt.; replacing water prior to the implantation by a smaller concentration of a water-miscible solvent, such as glycerol or dimethylsulfoxide; or implanting the lens in the state in which the iogenic groups are not fully ionized, i.e. in the acidic state prior to the neutralization, and letting the neutralization proceed spontaneously in situ by positive ions from the body fluids. The lens achieves its osmotic equilibrium spontaneously in hours to days after the implantation.

The lens shape is being formed preferably by crosslinking copolymerization of methacrylic and/or acrylic esters and salts in the closed two-part mold.

The shape of the lens can be adjusted after the molding by removing some part of the lens, e.g. by cutting off part of the supporting part, by drilling the lens outside the optical zone etc. The shape adjustment can be made in the hydrogel or the xerogel (i.e. non-hydrated) state. We have found that the negatively charged hydrogel material even allows use of methods developed primarily for living tissues (incl. NCR), such as ultrasonic phacoemulsification, cauterization or femtosecond laser treatment. These methods allow shape adjustment even in the fully hydrated hydrogel state. The femtosecond laser may be used even for formation of cavities inside the hydrogel lens that can be used to form new refractive members in the lens, for instance as a refractive cylindrical lens for astigmatism compensation. In the case that the matter removed by the shape adjustment (e.g., by a laser treatment) is water-soluble and substantially non-toxic, such an optical adjustment can be conceivably achieved even post-operatively in situ. The composition of the hydrogel in at least the treated part of the lens should be advantageously based on esters of polymethacrylic acid. It is known that such polymers are capable of depolymerization to their parent monomers (such as 2-hydroxyethyl methacrylate or methacrylic acid) that are well soluble, easily diffusible compounds of low toxicity. Other polymers, such as polyacrylates, polyvinyl compounds or polyurethanes do not have this advantage.

The invention is further illustrated by the following Examples that are meant to provide additional information without limiting the scope of the invention.

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EXAMPLE 1:

The following monomer mixture was prepared: 98 weight parts of 2-hydroxyethyl methacrylate (HEMA), 0.5 wt% of triethyleneglycol dimethacrylate (TEGDMA), 1 wt% of methacryloyloxybenzophenone (MOBP), 1 wt% of methacrylic acid, 0.25 wt% of camphorcquinone (CQ) and 0.05 wt% of trieathanolamine (TEA). The mixture was de-aired using by carbon dioxide and filled into two-part plastic molds shown schematically in Fig. 8 where 18B is the part of the mold for molding the a posterior lens surface, 18A is the part of the mold to shape the anterior part of the surface of the lens. Both parts are injection molded from polypropylene (PP). The shaping surface 19B of the part 18B has shape formed by two concentric hyperboloids. The central part of the surface has the diameter 3 mm, central radius of 3.25 mm and conic constant -3.76 while the peripheral is hyperboloid with central radius of 3.25 mm and conic constant -6.26. The molding surface is equipped with a protruding circular barrier 20 on diameter 8.5 mm that has asymmetric triangular profile, height 0.2 mm. This lip is designed to shape the connecting surface 6 in Fig. 3 A.

The part 18A has the shaping surface 19A that is divided into the central part 21 of diameter 6.8 mm and the peripheral part 22A of the diameter 13 mm. The peripheral part is formed by a hyperboloid with the central radius 3.25 mm and the conic constant -6.26. The peripheral hyperbolic surface is parallel to the corresponding surface of the part 18B. The central portion of the part 18A has the central radius of curvature -20 mm and conic constant h=l.

About 0.1 ml of the monomer mixture is pipetted into the part 18B, then it is covered by the part 18A that is carefully centered and pressed gently against it by a small weight. The only direct contact between the parts is the circular contact between the barrier 20 and the peripheral part of 22A. The mold is then illuminated for 10 minutes by a blue light at the wavelength 471 nm. The light initiates polymerization of the monomers accompanied by gelling at a relatively low conversion and by volume contraction that is roughly proportionate to conversion. The contraction of the soft gel creates a mild vacuum that pulls both parts of the mold together. The conical peripheral part 22 A of the mold 18A presses against the barrier 20, deforms it slightly and closes to the part 18B to reduce the volume of the molding cavity. This compensates for the volume shrinkage due to the polymerization. The described mold design is particularly suitable for production of relatively bulky IOLs from materials with high polymerization contraction that achieves gel-point at a relatively low conversion.

The mold parts are separated and the xerogel lens, the exact copy of the mold cavity, is neutralized by solution of sodium bicarbonate and extracted with isotonic solution. The

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PCT/IB2017/000911 linear expansion factor between the xerogel and hydrogel lens is 1.17. After evaluation of optical properties the lens was immersed in the 18% by weight aqueous solution of NaCI in a sealed blister package and sterilized by autoclaving.

EXAMPLE 2:

The following monomer mixture was prepared: 94 weight parts of 2-hydroxyethyl methacrylate (HEMA), 0.5 wt% of triethyleneglycol dimethacrylate (TEGDMA), 4.5 wt% of methacryloyloxybenzophenone (MOBP), 1 wt% of methacrylic acid and 0.25 wt% of dibenzoylperoxide. The mixture was de-aired using nitrogen carbon and filled into two-part plastic molds shown schematically in Fig. 8. The shaping surface 19B of the part 18B has a shape formed by two concentric surfaces. The central part of the surface has the diameter 3 mm, central radius of 3.00 mm and conic constant 1 while the peripheral section is a hyperboloid with central radius of 3.25 mm and conic constant -6.26. The molding surface is equipped with a protruding circular barrier 20 on diameter 8.8 mm that has asymmetric triangular profile, height 0.15 mm. The inner side of the barrier 20 is designed to shape the connecting surface 6 in Fig. 3 A.

The part 18A has the shaping surface 19A that is divided into the central part 21 of diameter 7.1 mm and the peripheral part 22A of the diameter 13 mm. The peripheral part is formed by a hyperboloid with the central radius 3.25 mm and the conic constant -6.26. The peripheral hyperbolic surface is parallel to the corresponding surface of the part 18B. The central portion of the part 18A is a plane perpendicular to the optical axis 1 A.

About 0.1 ml of the monomer mixture is pipetted into the part 18B, then it is covered by the part 18A that is carefully centered and pressed gently against it by a small weight. The only direct contact between the parts is the circular contact between the barrier 20 and the peripheral part of 22A. The mold is then heated to 75°C for 6 hours.

The mold parts are separated and the xerogel lens, the exact copy of the mold cavity, is neutralized by solution of sodium bicarbonate and extracted 3 times with ethyl alcohol and 5 times with isotonic solution. The lens was yellow with complete absorption of UV light and part of the blue visible light. The linear expansion factor between the xerogel and hydrogel lens is 1.13. After evaluation of optical properties the lens was immersed in the 15% by weight aqueous solution of NaCI in a sealed blister package and sterilized by autoclaving.

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EXAMPLES:

The following monomer mixture was prepared: 94.5 weight parts of 2-hydroxyethyl methacrylate (HEMA), 0.5 wt% of triethyleneglycol dimethacrylate (TEGDMA), 5 wt% of methacryloyloxybenzophenone (MOBP) and 0.25 wt% of dibenzoylperoxide. The mixture was de-aired using nitrogen carbon and filled into two-part plastic molds shown schematically in Fig. 8. The shaping surface 19B of the part 18B has a shape formed by two concentric surfaces. The central part of the surface has the diameter 6.5 mm, central radius of

4.5 mm and conic constant 0 while the peripheral section is a hyperboloid with central radius of 4.25 mm and conic constant -8. The molding surface is equipped with a protruding circular barrier 20 on diameter 9.3 mm that has asymmetric triangular profile, height 0.35 mm. The inner side of the barrier 20 is designed to shape the connecting surface 6 in Fig. 3 A.

The part 18A has the shaping surface 19A that is divided into the central part 21 of diameter 6.4 mm and the peripheral part 22A of the diameter 13 mm. The peripheral part is formed by a hyperboloid with the central radius 4.25 mm and the conic constant -8. The peripheral hyperbolic surface is parallel to the corresponding surface of the part 18B. The central portion of the part 18A is a surface of diameter 6.4 mm, central radius -3.75 mm and conic constant -6.

About 0.1 ml of the monomer mixture is pipetted into the part 18B, then it is covered by the part 18A that is carefully centered and pressed gently against it by a small weight. The only direct contact between the parts is the circular contact between the barrier 20 and the peripheral part of 22A. The mold is then heated to 75°C for 6 hours.

The mold parts are separated and the xerogel lens, the exact copy of the mold cavity, is extracted. The lens is then treated by a quaternary base as described in the reference Stoy ‘208.

The z lens from the clear, electroneutral crosslinked hydrophilic polymer has a surface created by a gradiented layer with high hydration and negative charge density. The lens was neutralized by solution of sodium bicarbonate and extracted 3 times with ethyl alcohol and 5 times with isotonic solution. The lens was clear with complete absorption of UV light. The linear expansion factor between the xerogel and hydrogel lens is about 1.12. After evaluation of optical properties the lens was immersed in the isotonic aqueous solution of NaCl in a sealed blister package and sterilized by autoclaving.

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EXAMPLE 4:

Raman spectra of the hydrogels of the invention show a significant difference between the original hydrogel and the same hydrogel subjected to TP A by exposure to the focused beam of a femtosecond laser. Namely, there is a significant difference in the ratio of the signal at 3420 cm'¹ corresponding to water, and at 2945 cm'¹ corresponding to the CEE group from the polymer backbone (see Fig. 9A). The ratio between the intensities of the two peaks is proportional to the phase-shift (in number of wavelengths) of the test laser beam between the modified and unmodified material (see Fig 9B). The Raman scan across the modified strip within the hydrogel showed increased water content in the treated area (see Fig 9C). On the other hand, the Raman spectrum of the region below 2000 cm'¹ showed no indication of new chemical groups. This is consistent with replacement of part of the polymer mass for an aqueous liquid through a mechanism such as depolymerization.

These and other advantages of the present invention will be apparent to those skilled in the art from the foregoing specification. Accordingly, it will be recognized by those skilled in the art that changes or modifications may be made to the above-described embodiments without departing from the broad inventive concepts of the invention. It should therefore be understood that this invention is not limited to the particular embodiments described herein, but is intended to include all changes and modifications that are within the scope and spirit of the invention as defined in the claims.

Claims (55)

1. A covalently crosslinked hydrogel comprising a polymer comprising monomer units of (meth)acrylic acid derivatives and/or (meth)acrylic acid and comprising UV-absorbing dopant moieties and activator moieties that are negatively charged at physiological pH, wherein exposure of the fully hydrated hydrogel to electromagnetic radiation results in two-photon absorption which causes one or more structural changes in the hydrogel and a negative change in the refractive index.

2 The hydrogel according to Claim 1 wherein the dopant moieties and activator moieties are pendant groups on a polyacrylate or polymethacrylate polymer.

3. The hydrogel according to Claim 1 wherein the dopant moiety is a UV-absorbing compound that does not strongly absorb light of about 400 nm wavelength.

4 The hydrogel according to Claim 1 wherein the dopant moiety is a compound selected from the group consisting of rhodamines, benzophenones, coumarins, fluoresceins, benzotriazoles, and derivatives thereof.

5. The hydrogel according to Claim 1 wherein the activator moiety is a compound comprising a carboxylate group, sulfonate group, sulfate group or phosphate group.

6. The hydrogel according to Claim 1 wherein said one or more structural changes comprise partial depolymerization of said hydrogel.

7. The hydrogel according to Claim 6 wherein said partial depolymerization forms an aqueous-filled void in said hydrogel.

8. The hydrogel according to Claim 6 wherein the depth of said partial depolymerization of the hydrogel depends on the cumulative energy absorbed in a given location of the hydrogel.

9. An ophthalmic implant comprising the hydrogel according to claim 1.

10. An in .«///-adjustable crosslinked hydrogel ophthalmic implant comprising an acrylate or methacrylate copolymer hydrogel wherein said copolymer comprises at least four co-monomers:

a) an acrylate or methacrylate ester containing at least one pendant hydroxyl group;

b) a polyol acrylate ester or amide or polyol methacrylate ester or amide with at least 2 acrylate or methacrylate groups per polyol ester or amide;

c) a derivative of acrylic or methacrylic acid having at least one pendant carboxyl group; and

d) a vinyl, acrylic or methacrylic monomer having a pendant UV-absorbing group;

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PCT/IB2017/000911 wherein the refractive properties of said implant are adjusted by a controlled absorption of targeted electromagnetic radiation by said hydrogel resulting in a negative change of refractive index in selected locations of said implant.

11. The hydrogel ophthalmic implant according to Claim 10 wherein the implant has anterior and posterior refractive surfaces forming a lens with positive or negative refractive power.

12. The hydrogel ophthalmic implant according to Claim 11 wherein the lens is an intrastromal lens.

13. The hydrogel ophthalmic implant according to Claim 11 wherein the lens is an anterior chamber lens.

14. The hydrogel ophthalmic implant according to Claim 11 wherein the lens is a phakic lens for location between the iris and the natural crystalline lens.

15. The hydrogel ophthalmic implant according to Claim 11 wherein the lens is a posterior chamber lens for at least partial replacement of the natural crystalline lens.

16. The hydrogel ophthalmic implant according to Claim 11 wherein at least one of the refractive surfaces is an aspheric surface with negative spherical aberration.

17. The hydrogel ophthalmic implant according Claim 10 wherein the monomer containing the pendant carboxyl group is a neutralized or partially neutralized methacrylic acid.

18. The hydrogel ophthalmic implant according Claim 10 wherein the monomer containing the pendant carboxyl group is present in a concentration between 0.1 molar % and 5 molar % based on all monomer units of the co-polymer.

19. . The hydrogel ophthalmic implant according Claim 18 wherein the monomer containing the pendant carboxyl group is present in a concentration between 0.5 molar % and 2 molar % based on all monomer units of the co-polymer.

20. The hydrogel ophthalmic implant according Claim 10 wherein the monomer containing the pendant UV-absorbing group is present in a concentration between 0.1 molar % and 5 molar % based on all monomer units of the co-polymer.

21. The hydrogel ophthalmic implant according Claim 20 wherein the monomer containing the pendant UV-absorbing group is present in a concentration between 0.2 molar % and 2.5 molar % based on all monomer units of the co-polymer.

22. The hydrogel ophthalmic implant according Claim 10 wherein the pendant UV-absorbing group contains a phenolic hydroxyl group conjugated to an aromatic group.

23. The hydrogel ophthalmic implant according Claim 22 wherein the monomer containing the pendant UV-absorbing group is present in a concentration between 0.1 molar % and 5 molar % based on all monomer units of the co-polymer.

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24. The hydrogel ophthalmic implant according Claim 10 wherein at least one of the UVabsorbing pendant groups is selected from the group consisting of derivatives of benzophenone, derivatives of benzotriazole, derivatives of coumarin and derivatives of fluorescein.

25. The hydrogel ophthalmic implant according Claim 10 wherein the pendant carboxyl groups and pendant UV-absorbing groups are present in a molar ratio between about 0.25 and about 5.

26. The hydrogel ophthalmic implant according Claim 25 wherein the pendant carboxyl groups and pendant UV-absorbing groups are present in a molar ratio between about 0.5 and about 3.5.

27. The hydrogel ophthalmic implant according Claim 10 wherein the copolymer contains at least two different comonomers containing different UV-absorbing groups.

28. The hydrogel ophthalmic implant according Claim 27 wherein at least one of the UVabsorbing groups is a benzophenone or a derivative thereof.

29. The ophthalmic lens according to Claim 11 wherein the pendant carboxyl group is ionized, and the molar ratio of ionized pendant carboxyl groups to UV-absorbing pendant groups is from about 0.5 to about 3.5.

30. The ophthalmic lens according Claim 11 wherein at least the major portion of the polymer of said hydrogel is a hydrophilic derivative of methacrylic acid.

31. The ophthalmic lens according Claim 30 wherein at least a major portion of the hydrophilic methacrylic acid derivative is a glycol ester of methacrylic acid.

32. The ophthalmic lens according Claim 11 wherein the covalently crosslinked hydrogel contains more than 30% by weight of liquid under equilibrium physiological conditions.

33. . The ophthalmic lens according Claim 11 wherein the covalently crosslinked hydrogel contains less than 55% by weight of liquid under equilibrium physiological conditions.

34. The ophthalmic lens according Claim 11 wherein the covalently crosslinked hydrogel contains between 35% and 47.5% by weight of liquid under equilibrium physiological conditions.

35. The ophthalmic lens according Claim 11 wherein at least the posterior optical surface contributes refraction with negative spherical aberration.

36. The ophthalmic lens according Claim 11 wherein the posterior optical surface contributes refraction with negative spherical aberration between -0.1 microns and -2 microns.

37. The ophthalmic lens according Claim 36 wherein the negative spherical aberration is between -0.5 microns and -1.5 microns.

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38. The ophthalmic lens according Claim 37 wherein the negative spherical aberration is between -0.75 microns and -1.25 microns on an aperture >4.5 mm.

39. The ophthalmic lens according Claim 11 comprising both UV-absorbing pendant groups containing phenolic hydroxyl groups conjugated to an aromatic system as well as UV-absorbing groups containing a benzotriazole structure.

40. The ophthalmic lens according Claim 39 wherein said UV-absorbing pendant groups containing phenolic hydroxyl groups conjugated to an aromatic system and said UV-absorbing groups containing a benzotriazole structure are located in separate layers of the ophthalmic lens.

41. The ophthalmic lens according Claim 11 wherein the lens is implanted into a cornea.

42. The ophthalmic lens according Claim 11 wherein the lens is implanted into the anterior chamber of an eye between the cornea and iris.

43. The ophthalmic lens according Claim 11 wherein the lens is a phakic lens implanted between the iris and the natural crystalline lens.

44. The ophthalmic lens according Claim 11 wherein the lens is implanted into the posterior chamber of eye, at least partially replacing the natural crystalline lens.

45. A method of adjusting the refractive properties of a fully hydrated hydrogel of claim 1, said method comprising the step of focused irradiating said hydrogel with electromagnetic radiation such that two-photon absorption occurs, wherein the polymer component of said hydrogel undergoes partial depolymerization and/or decomposition.

46. A method of in situ adjusting the optical parameters of a hydrogel ophthalmic implant, said method comprising the steps of:

a) providing an eye containing a hydrogel ophthalmic implant according to claim 9; and

b) irradiating a portion of said hydrogel ophthalmic implant with electromagnetic radiation using a femtosecond laser, whereby part of the copolymer of said hydrogel is depolymerized and or decomposed;

wherein the optical parameters of said implant are adjusted.

47. The method of claim 46 wherein said optical parameters include the refractive index.

48. The method of claim 46 wherein said irradiating produces elongated cavities or voxels inside the hydrogel ophthalmic implant.

49. The method of claim 48 wherein said voxel depth is larger than 10 microns.

50. The method of claim 48 wherein increasing the depth of the voxels increases the phase shift while the refractive index remains approximately constant.

51. The method of claim 50 wherein said refractive index is greater than or equal to 1.3335.

52. The method of claim 50 wherein said phase shift is up to 30 microns.

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53. The method of claim 46 wherein the depolymerized matter ablated by said irradiation comprises soluble, easily diffusible compounds of low toxicity.

54. The method of claim 46 wherein said modified optical properties are provided by forming in said hydrogel a pattern of elongated voxels of varying depth while keeping the modified

5 refractive index approximately constant.

55. The method of claim 50 wherein said phase shift is controlled by varying voxel depth rather than by varying their refractive index.