AU2004201322A1 - Compositions and methods for treating viral infections - Google Patents

Description

P/00/011 Regulation 3.2

AUSTRALIA

Patents Act 1990 COMPLETE SPECIFICATION STANDARD PATENT Invention Title: Compositions and methods for treating viral infections The following statement is a full description of this invention, including the best method of performing it known to us: Freehills Carter Smith Beadle Sydney\004606092 Printed 29 March 2004 (14:44) page 3 COMPOSITIONS AND METHODS

FOR

TREATING VIRAL INFECTIONS Technical Field The present invention relates generally to the treatment and prevention of viral infections, In particular, the invention provides compositions and methods for the production of antibodies and peptides .useful in the treatment and diagnosis of human immunodeficiency virus (HIV) infections.

1 0 Background of the Invention The diagnosis, treatment and prevention of viral infections is a primary focus of many medical researchers. Although compositions and methods of diagnosing, treating and vaccinating against a number of. viral infections are known, there are still a number of viruses which are difficult to detect in man and for wnicn no effective methods of treatment or vaccination against are known. Of these, one of the most significant, of course, is HIV.

The infectious agent responsible for acquired immunodeficiency syndrome (AIDS) and its prodromal phases, AIDS-related complex (ARC) and lymphadenopathy syndrome (LAS), is a lymphotrophic retrovirus termed LAV,

HTLV-I

T I ARV, and recently HIV as recommended by the international Committee on Taxonomy of Viruses (Ref 299). Nomenclature herein employs these recommendations to designated viruses associated with AIDS and the strains thereof. Historic references to strains, which include LAV and ARV-2, are now. named HIV1 LAI and HIVlsr 2 respectively.

As the spread of HIV reaches pandemic proportions, the treatment of infected individuals and prevention of the transmission to uninfected individuals at risk of exposure is of paramount concern. A variety of therapeutic strategies have targeted different stages in the life cycle of the virus and are outlined in Mitsuya and Broder, 1987, Nature 325:773. One approach involves the use of antibodies which bind to the virus and inhibit viral replication, either by interfering with viral entry into host cells or by some other mechanism. Once the viral component(s) susceptible to antibody intervention are identified, it has been hoped that antibody reactivity sufficient to neutralize the infectivity of the virus could be generated and administered to HIV-infected patients in the form of immune globulins or purified antibodies and that this passive immunization procedure would alter or reverse progression of HIV infection. In addition, it has been hoped that the vaccination of non-infected individuals with selected epitopes modified to enhance

MHC

interactions would provide protection from subsequent infection following exposure to HIV.

The envelope glycoproteins of most retroviruses are thought to react with receptor molecules on the surface of susceptible cells, thereby determining the virus' infectivity for certain hosts. Antibodies that bind to these envelope glycoproteins may block the interaction of the virus with the cell receptors, neutralizing the infectivity of the virus. See generally, The Molecular Biology of Tumor Viruses, 534 Tooze, ed., 1973); and RNA Tumor Viruses, 226, 236 Weiss et al., eds., 1982); Gonzalez-Scarano et al., 1982, Virology 120:42 (La Crosse Virus); Matsuno and Inouye, 1983, Infect. Immun. 39:155 (Neonatal Calf Diarrhea Virus); and Mathews et al., 1982, J. Immunol., 129:2763 (Encephalomyelitis Virus). To date, therapeutic strategies directed at eliciting protective immune responses in man by vaccination with

HIV

proteins/peptides have failed. In addition, neither high titer neutralizing antibodies recovered from

HIV-

infected patients nor monoclonal antibodies produced in mice have succeeded in altering the progression of HIV infection to AIDS and death. There is a need in the art to identify alternate immunological targets on HIV which will elicit immune responses that will modify the course of HIV infection.

The general structure of HIV is that of a ribonucleo-protein core surrounded by a lipidcontaining envelope which the virus acquires during the course of budding from the membrane of the infected host cell. Embedded within the envelope and projecting outward are the viral encoded glycoproteins. The envelope glycoproteins of HIV are initially synthesized in the infected cell as a precursor molecule of 150 ,000-160,000 Daltons (gp 160), which is then processed in the el nto an N-terminal fragment of 110,000-120,000 Dalzons (gp 1204 to generate the external glycoprotein, and a C-terminal fragment of 41,000-46,000 Daltons (gp 41), which is the transmembrane envelope glycoprotein.

For the reasons discussed above, the gp 120 glycoprotein of HIV has been the object of much investigation as a potential target for interrupting the virus' life cycle. Sera from HIV-infected individuals have been shown to neutralize HIV in vitro, and antibodies that bind to purified gp 120 are present in these sera, (Robert-Guroff et al., 1985, Nature 316:72; Weiss et al., 1985, Nature 316:69; and Mathews et al., 1986, Proc. Natl. Acad. Sci. 83:9709).

Purified and recombinant gp 120 stimulated the production of neutralizing serum antibodies when used to immunize animals (Robey et al., 1986, Proc. Natl.

Acad. Sci. 83:7023; Lasky et al., 1986, Science, 233:209) and a human (Zagury et al., 1986, Nature 326:249). Binding of the gp 120 molecule to the CD4 receptor also has been shown and monoclonal antibodies which recognize certain epitopes of the CD4 receptor have been shown to block HIV binding, syncytia formation, and infectivity. McDougal et al., (1986, Science 231:382) and Putney et al. (1986, Science 234:1392) elicited neutralizing serum antibodies in animals after immunizing with a recombinant fusion protein containing the carboxyl-terminal half of the gp 120 molecule and further demonstrated that glycosylation of the envelope protein is unnecessary for a neutralizing antibody response.

Shortly after HIV infection the immune system of man responds to the virus with both antibody production and cell mediated immune responses.

A

review of the immune responses to retroviruses has been published (Norey, and Kurth 1994: The Retroviridae, Vol E, J.A. Levy, ed., pp. 363-464, Plenum Press). Human antibodies specific for a number of HIV proteins including gp 160, gp 120, p66, p55, gp 41, p32, p 2 4, and p7 have been reported (Carlson 1988, J.Am. Med. Assoc. 206:674). The initial antibody response in man to HIV is directed to p17 and p24, followed by gp 120/160, then by gp 41, p66/55 and finally p 3 2 (Lange 35 et al 1986, Br. Med. J. 292:228) As HIV infection progresses into AIDS antibody levels to p17 and p24 markedly fall to undetectable limits and are replaced by p17 and p24 antigenemia. Antibody titers to p32 and p55 also decline but to a lesser degree (McDougal et al 1987 J. Clin. Invest. 80:316).

However, substantial amounts of antibodies to go 160/120 persist throughout the entire course of HIV infection. During the early phases of HIV infection an elevation in total immunoglobulins is observed and this increased quantity of antibody is specific for HIV and.

predominantly directed to gp 120, (Amadori et al., 1988 Clin. Immunol. Immunopathol. 46:342; Amadori et al, 1989, J. Immunol 143:2146). Possible mechanisms for this HIV specific hyper.gamma globulinemia have been reviewed by Barker E. et al 1995: The Retroviridae Vol 4, J.A. Levy, ed. pp 1-96 Plenum Press. Functional properties and epitopes targeted by these antibodies produced during HIV infection have been described and include epitopes which are susceptible to antibody mediated neutralization. These primary target epitopes are primarily located on the envelope protein (gpl 2 0/gp41) and the gag protein pl 7 for review see Levy, 1994 Am.Soc. Micro; Nixon et al, 1992 Immunol 6:5o15. Neutralizing antibodies to HIV envelope protein have been identified and bind to conserved and divergent domains on gp 120. These include regions localized to the CD4 binding regions (Linsley et al 1988 and Thali et al, 1992); the second and third variable loop domains (Fung et al, 1992 and Haigwood et al 1990); and carbohydrate moieties (Benjouad et al, 1992 and Feizi and Larkin, 1990). Other neutralization .sites have been identified on the external portion of gp 41 and a binding site on p17 (Changh et al, 1986) Early studies suggested that the presence of neutralizing antibodies lead to a more favorable clinical outcome, (Robert-Guroff et al, 1985).

However, these studies employed selected sera with high neutralizing capacity against laboratory strains of HIV and not against autologous HIV isolates (Homsy et al, 1990; Tremblay and Wainberg, 1990). Subsequent investigation demonstrated that autologous antibody had little or no neutralizing activity against autologous HIV isolates (Homsy et al, 1990). The lack of susceptibility to antibody mediated neutralization in the presence of a neutralizing antibody is thought to result from the development of escape mutants that appear after seroconversion (Arendrup et al, 1992) and throughout the infection as new antibody specificities are produced, The clinical relevance of neutralizing antibodies produced as a consequence of HIV infection is unclear. However, it is clear that in spite of a vigorous immune response to HIV in individuals infected with HIV, progress to AIDS and, ultimately, death as a consequence of immune dysfunction predominates. Accordingly, new methods of treatment are sought.

It is an object of this invention to identify neutralizing regions of viral proteins which fail to elicit immune responses in man but do elicit immune responses in non-human mammals and to produce antibodies reactive with these regions. It is a further object of this invention to use these identified neutralizing regions of proteins and the antibodies reactive with them in the diagnosis, treatment and prevention of disease caused by the virus. Further objects of this invention will be apparent from the description of the invention detailed below.

The applicant does not concede that the prior art discussed in the specification forms part of the common general knowledge in the art at the priority date of this application.

Summary of the invention According to a first aspect of the invention there is provided a composition comprising at least one protein or a fragment thereof, of a pathogen, wherein the protein or fragment thereof comprises at least one epitope region which does not elicit an immune response in a first animal species when encountered through infection or environmental exposure but does elicit an immune response in at least one second animal species. In a preferred embodiment one animal species is man.

According to a second aspect of the invention there is provided a composition comprising at least one synthetic peptide, the or each peptide comprising at least one epitope region which corresponds to or mimics a neutralising or inactivating region of a protein of a pathogen, wherein the peptide(s) does not elicit an immune response in a first animal species when encountered by infection or environmental exposure but does elicit an immune response in at least one second animal species.

The invention also provides for an immunomodulator comprising a polymer or copolymer of glycopeptide(s) extensively cross-linked and in the form of microparticles. Preferably these microparticles comprise multiple repeats of muramyl dipeptide. There is further provided a method of administering an immunomodulatory effective amount of the immunomodulator to a non-human animal to modulate the immune system of the animal.

According to another aspect of the invention there is provided a macromolecular carrier comprising a polymer or a copolymer of glycopeptide(s) extensively crosslinked, in the form of microparticles, the or each monomer of the glycopeptide(s) capable of binding at least one immunogenic epitope. In a preferred embodiment the invention provides a composition containing a cocktail of macromolecular carrier species, each carrier species supporting a different immunogenic epitope.

According to a further aspect of the invention. there is provided a method of identifying a neutralising or inactivating region of a protein of a pathogen, wherein the neutralising or inactivating region does not elicit an immune response in man when encountered by infection or environmental exposure but does elicit an immune response in a non-human animal. The method includes the steps of presenting the protein or part thereof to an immune system of a non-human animal,obtaining an antibody preparation after presentation with the protein or part thereof, employing the antibody preparation in a competitive immunoassay with a human antibody preparation to the protein or part thereof to identify regions of the protein or part thereof which are recognised by antibodies in the antibody preparation from the nonhuman animal but not recognised by antibodies in the human antibody preparation, and determining which of these regions is a neutralising or inactivating region.

The invention further provides for a method for obtaining antibodies which react with an epitope on a neutralising or inactivating region of a protein of a pathogen, wherein the neutralising or inactivating region of the protein fails to elicit an immune response in man when encountered by infection or environmental exposure but does elicit an immune response in a non-human animal. The method includes the steps of isolating proteins from a lysate of the pathogen, identifying an epitope on at least one of the proteins which has an amino acid sequence which corresponds to or mimics the amino acid sequence of a neutralising or inactivating region which fails to elicit an immune response in man when encountered by infection or by environmental exposure but does elicit an immune response in a non-human animal, presenting the protein or part thereof to an immune system of said non-human animal, and obtaining antibodies to said epitope from said non-human animal. It will also be appreciated that this method may alternatively comprise the steps of synthesising a peptide having an amino acid sequence which corresponds to or mimics an epitope on a neutralising or inactivating region of the protein, wherein the region fails to elicit an immune response in man when encountered by infection or environmental exposure but does elicit an inmmune response in a non-human animal, presenting the peptide to an immune system of said non-human animal, and obtaining antibodies to the epitope from said non-human animal.

According to another aspect of the invention there is provided an antibody, or combination of at least two antibodies, which recognises and reacts with an epitope which corresponds to or mimics an epitope on a neutralising or inactivating region of a protein of a pathogen, wherein the neutralising or inactivating region of the protein fails to elicit an immune response in man when encountered by infection or environmental exposure. It will also be appreciated that such an antibody may be used in a method for purifying protein containing at least one epitope which corresponds to or mimics an epitope of a neutralising or inactivating region of a protein from a protein solution, wherein the antibody is immobilised to a substrate or solid support, the immobilised antibody is contacted with a solution containing the protein under conditions suitable for the formation of immune complexes between the antibody and the protein, unbound protein is separated from protein bound to the antibody, and bound protein is released from the antibody and recovered.

The antibodies of the invention can be used in methods of detecting the presence of a pathogen in a biological sample from an individual who may have been infected with the pathogen, whereby the method comprises an antibody-antigen assay in which the antibody is combined with a sample of body fluid from the individual under conditions conducive to antibody-antigen complex formation and determining whether the antibody binds to an antigen of the pathogen. In another embodiment the method of detecting the presence of a pathogen in a biological sample from an individual who may have been infected with the pathogen is an enzyme immunoassay wherein the antibody is conjugated to an enzyme on contact with a sample of body fluid from the individual under conditions conducive to antibody-antigen complex formation and determining whether the antibody binds to an antigen of the pathogen.

According to another aspect of the invention there is provided a method of inhibiting or preventing an infection with a pathogen in a non-human animal, the methods comprising administering to the animal a therapeutically effective amount of a composition including one or more antibodies each of which recognises and reacts with an epitope which corresponds to or mimics an epitope on a neutralising or inactivating region of a protein of the pathogen, wherein the neutralising or inactivating region of the protein fails to elicit an immune response in the animal. It will also be appreciated that this method may be used for neutralising or inactivating one or more essential steps in the life cycle of a pathogen in a non-human animal infected with the pathogen.

The invention further provides for methods for vaccinating a non-human animal against infection with a pathogen, or inhibiting or preventing infection with the pathogen, wherein a composition comprising one or more peptides including at least one site which corresponds to or mimics an epitope on a neutralising or inactivating region of a protein of the pathogen is administered to the animal, wherein the neutralising or inactivating region of the protein fails to elicit an immune response in the animal when encountered by infection or environmental exposure, the composition administered in an amount sufficient to stimulate the production of antibodies against the or each epitope.

Detailed description of the invention The present invention provides novel compositions and methods for diagnosing and neutralizing viral infections. The invention will be described in detail with a focus on a preferred embodiment, in which the virus of interest is HIV. It is to be understood, however, that the principles of the invention can be used to identify neutralizing regions of proteins of other viruses and to produce antibodies reactive with those proteins that can be used to diagnose, treat and prevent infections caused by these other viruses as well.

Focusing now on HIV, this invention provides novel compositions and methods for neutralizing HIV infection and preventing or substantially inhibiting HIV infectivity, cell to cell transmission, and virus production in the infected host. More specifically, HIV protein sequences containing epitopes which fail to elicit an immune response in man when encountered through infection or naturally through the environment are utilized as described in detail below to produce antibodies in non-human mammals which can be administered to neutralize HIV infectivity, facilitate killing of infected CD4 lymphocytes, and inactive essential steps in the life cycle of HIV. The term "neutralizing region" indicates those portions of HIV, particularly HIV proteins, containing amino acid segments defining one or more epitopes reactive with antibodies which, either individually or in combination with other antibodies of the present invention, are capable of neutralizing HIV infections. Suitable assays for evaluating neutralization are well known and can include assays which measure reduction of HIV infections in T-cell lines, reduction of plaque forming units of VSV (HIV) pseudo types bearing the envelope glycoproteins of HIV, syncytial inhibition tests, and virion-receptbr binding tests. The term "inactivating region" indicates those segments of HIV proteins which contain one or more epitopes which when reacted with antibodies of this invention, either individually or in combination, inactivate functionally important events in the life cycle of HIV. Suitable assays to evaluate antibody-mediated destruction of HIV infected lymphocytes are well known and can include antibody dependent cell mediated cytotoxicity, complement mediated lysis, and natural killer (NK) assays.

Suitable assays for measuring antibody-mediated inactivation of essential steps of the life cycle of HIV include assays which determine inactivation of reverse transcriptase, or measure polymerase and protease activity, or which evaluate antibody-mediated complement dependent changes in nuclear capsid permeability exposing viral RNA to ribonuclease degradation. As desired, the neutralizing activity can be compared to antibody reactivity in immunochemical assays, such as immunofluorescence, immunoblot, enzyme linked immunoassay, and radioimmunoassay.

The present invention is based on the discovery that epitopes which are functionally important in the life cycle of HIV, but not immunogenic in man, can be identified and characterized employing antibodies produced in selected mammalian species other than man.

In addition, it has been discovered that the immunological non-responsiveness in man to these regions is a function of molecular mimicry and lack of MHC associated events, including antigen presentation through required MHC HLA class 1 and HLA class 2 associated events. With molecular mimicry the epitope is seen as self and is not responded to under normal circumstances. With lack of MHC associated events in antigen presentation several steps are involved and failure of any one step can result in the absence of an immunological response to the antigen.

Peptide regions containing multiple overlapping epitopes and antibodies to these epitopes have been produced and are shown to neutralize and inactivate essential steps in the life cycle of HIV in vitro. In.

addition, HIV-infected patients with AIDS have been treated with antibodies to these peptide regions, resulting in rapid reduction in blood born infectivity measured by total culturable infectious dose

(TCID)

Treatment of chronic AIDS patients with these antibodies has resulted in marked clinical improvement, including weight gain, resolution of opportunistic infections, decreased incidence and severity of infections and doczor visits and resolution of HIVassociated neuropathy. The patients further have shown immunological recovery, as defined by a reduction of HIV-RNA, increased CD4 number, increased CD8 number and restoration of the cytokine system associated with improved CD4 and CD8 numbers and function.

Identification of Eirone and Production of nrtibodis The majority of immune reactions target immunodominant epitopes. HIV epitopes are most frequently identified and mapped by various immunological methods which employ antisera to HIV, cytotoxic T lymphocyte reactivity to HIV epitope targets and helper lymphocyte antigen presentation of HIV epitopes. 'Synthetic peptides of known sequence which mimic HIV sequence can be employed competitively or noncompetitively using well known assays to confirm these observations. It is to be understood that stimulation of the immune system can lead either to enhancement or suppression of the immune response.

Factors which govern this include: A. The sub population of lymphocytes stimulated by the immunogen (Suppressor versus Helper).

B. The micro-environment, including cell population residing therein, which contact the immunogen first.

C. The type of cytokines present in the micro-environment at the time of effector cell contact with the immunogen.

D. The type of cytokines elicited following effector cell contact with the immunogen.

E. Structural and biochemical composition of the immunogen.

F. The amino acid sequence of the immunogen and its susceptibility to protease degradation by proteases in the micro environment.

From preliminary experiments, the following properties were determined to be fundamentally important in determining the potential value of certain proteins and peptides for use in the production of antibodies which are intended for passive immunotherapy application in the treatment or palliation of disease processes in man and, in particular, infection with HIV at all stages including

AIDS:

A. The immunogen must lack epitope determinants that are expressed on human cells and tissues when employed to produce antibody for use in passive immunotherapy with .the following exceptions: 1. Antigen distribution is restricted to sequestered and/or unavailable locations to the antibody.

The antigen is expressed during developmental phases which permit the use of antibody at specific times during the developmental cycle, when antigen is not available.

3. Epitope location within the host is not adjacent to a vital structure.

4. Antigen distribution on host cell is at a density lower than required to produce injury but favorable on the desired target resulting in selective targeting.

The target to normal ratio must be sufficiently different and favor antibody delivery to the desired target.

B. The number of peptide repeats delivered to an antigen-presenting cell directly influences the magnitude of the immunological response.

C. The epitope must not be present in body fluids at concentrations which would neutralize antibody and prevent targeting.

To date vaccine development has focused on designing better technology to amplify responses to targets to which the immune system of man responds, and passive immunotherapy has resulted in inconclusive results. Disclosed herein are alternate targets on HIV which do not elicit immune events in man as well as a new configuration to deliver antigen which results in immune reactions to HIV not previously attainable. The methods disclosed herein focus on the treatment of HIV and AIDS, but it is to be understood that the formulations of this invention have broad application.

The antibody response in goats demonstrates utility of invention by way of antibody production to key targets on HIV, and by way of treatment which results in clinical improvement of AIDS. This technology has broad application in vaccine development.

Successful immune induction to antigen challenge requires the presentation of multiple epitope repeats by an antigen-presenting cell (APC) through MHC events.

Epitopes which are most immunogenic are in an amphipathic configuration with a hydrophobic amino acid on one terminus, a hydrophillic amino acid on the other terminus, contain amino acids consistent with the formation of amphipathic helices; they lack helix breaking amino acids, such as proline, and lack carbohydrate. Sequences which lack amino acids that are susceptible to protease degradation by proteases in the micro-environment are especially desired.

To identify immunological targets on HIV with functional importance, immunogenic regions on HIVrelated proteins in animal species other than man.were determined. Goats were immunized with purified

HIV

lysate with and without carbohydrate groups removed.

Removing carbohydrate residues from HIV proteins has little effect on the immunological response to the proteins yet can expose hidden epitopes. HIV lysates obtained commercially were further purified to remove proteins of tissue culture origins including human

HLA

class 1 antigens, HLA class 2 antigens, and beta-2microglobulin. Following immunization, antisera from the goats were tested employing competitive immunoassay methodology to identify HIV peptides not recognized by antibodies pooled from HIV-infected patients. Pools of human HIV antisera were prepared from selected patient sera with high neutralizing and Western Blot activity and employed as competitive antibodies using standard competitive immunoassay methods. A broad spectrum of goat antibodies were .identified which reacted with HIV determinants immunologically distinct from those .recognized by the human anti-HIV antisera pools.

Those skilled in the art recognize that other animal species could be used to produce antibodies to these epitopes and that such antibodies could function in ADCC and complement mediated reactions. Other suitable animal species for the production of antibodies include, but are not limited to, sheep, rabbits, horses, cows and mice.

The epitope reactivity of the anti-HIV antibodies was characterized using twelve-mer peptides spanning the linear amino acid sequences of HIVlsn. Peptides of this size react well with antibodies, can be synthesized easily and can be prepared in highly purified form. Peptides were synthesized by and purchased from Purification Systems, Inc. The synthetic peptides were combined with peroxidase labeled goat anti-HIV antibodies and combined with each of two sets of microtiter wells coated with HIV. One set was blocked with human IgG anti-HIV; the other set was not. The percent of peptide inhibition of goat anti-HIV binding to HIV protein sites blocked with human anti-HIV was determined.

When inhibition of binding was observed with a specific synthetic peptide, additional peptides were synthesized with amino acid sequences overlapping that of the original inhibiting peptide to further define the epitope sequences.

The location of the epitopes on the HIV proteins recognized by goat anti-HIV IgG but not human anti-HIV IgG were further evaluated and confirmed using

HIV

peptide-HRP conjugates as the identification markers.

In this assay, HIV proteins were absorbed to supports such as microtiter plate wells or precision polystyrene beads. Twelve-mer peptides spanning the linear amino acid sequence of HIVs 2 were covalently attached to horseradish peroxidase. Human and goat anti-HIV reactivity were meas.ured independently with anti-HIV reactivity from human and goat bridging the native epitopes adsorbed to the support and to the peptide epitope covalently attached to peroxidase. With this procedure, detailed in Example 8, only exact epitopes contained within the synthetic peptide were recognized.

Once peptides having significant mimicry with human proteins have been identified, those sequences which have functional importance in the life cycle of HIV are determined. This is done, as described below and illustrated in Example 8, by generating antibodies to candidate peptides and then testing those antibodies for their effects on HIV infectivity and viral neutralization.

As noted above, a number of specific epitope regions have been identified and nine are described in detail below with reference to the HIVlSF 2 sequence unless otherwise in--cated. Amino acid residue designations set for:h below and throughout this application for HIVi1-. are from the Los Alamos Data Bank (AIDS Virus Sequence Data Base, Los Alamos National Laboratories, Theoretical Division, Los Alamos, N.M. 87545). Amino acid residue designations set forth below and throughout this application for HIV2NZ are from the Ex Pasy World Wide Web Molecular Biology Server of the Geneva University Hospital and the University of Geneva, and the BioAccelerator available through Compugen Ltd. at the Weizman Institute, Israel, and Akira Ohyama, BioScience Systems Department, Mitsuey Knowledge Industry Co., Ltd., Tokyo, Japan. Those skilled in the art will appreciate that additional analogous regions ("homologs") from other HIV isolates can be identified based upon their location within related proteins from various isolates.

In practice, such homologs can be identified by reference to HIVlsF2 sequence data as follows: the amino acid sequences of HIV isolates and HIVISF2 can be aligned to obtain maximum homology between the two sequences, generally at least about identify between the sequences; S(b) once an amino acid sequence is aligned to the corresponding location within

HIVSF

2 proteins will demonstrate immunological mimicry, similarity, or identity with HIV1~- 2 as defined by retention of antibody reactivity to the mimicked or homologous sequence. Peptides from other HIV isolates and their amino acid sequences so identified typically will immunologically mimic corresponding regions on HIV1, 2 This method of identifying key epitopes can be applied to HIV strains that are yet to be discovered.

For example, as new strains of HIV are identified, their enyelope and core amino acid sequences can be aligned with that of HIV1s, to obtain maximum sequence homology with that strain. The methods by which the sequences.are aligned are known to those skilled in the art. in aligning the sequences it is desired to maintain as much homology between cysteine residues as possible. The amino acid sequence(s) of the new

HIV

strain or species which corresponds to the location of the peptides specifically disclosed herein can be synthesized and used in accordance with the invention.

it is not necessary to the present invention that the epitopes contained within such sequences be crossreactive with antibodies to all strains or species of HIV. Peptides encompassing immunological epitopes which distinguish one species or serogroup over another will find utility in identifying particular species or serogroups and may assist in identifying individuals infected with one or more species or serogroups of HIV.

They also can be useful in combination with other peptides, from either a homologous region or another neutralizing region, in therapeutic regimens,.

The amino acid sequences of this invention typically comprise from about 5 to about 50 amino acids and comprise an epitope region or multiple epitope regions located on HIV proteins that fail to elicit a protective immune response in man when encountered through infection or environmental contact but do elicit a response in a non-human mammal. Preferably, the sequences comprise between about 5 and 35 amino acids. Synthetic peptides or treated lysates of natural HIV proteins containing the desired amino acid sequences are used to immunize animals which respond immunologically to them and produce antibodies which have therapeutic value in treating HIV infections.

The amino acid sequences or peptides of interest fail to elicit an immune response in man through mimicry of epitopes on human and other proteins. Of particular interest are peptide epitopes shared between HIV proteins and human alpha fetoprotein, aspartyl protease, deoxyuridine nucleotidohydrolase, eosinophil cationic protein, eosinophil-derived neurotoxin and ribonuclease 4 precursor and peptide epitope regions mimicked by neurotoxins from Bungaris Naja, Dendoaspis, Psudechis, or Androctonus Centruroides.

In the discussion which follows, reference is made to a number of human proteins and neurotoxins using 18 standard identifying abbreviations for the proteins.

Set forth below is a table which sets forth these abbreviations and the full names of the proteins to which they correspond: Human Proteins' jjth S6quence.Similarity to EIV Proteins Swiss Prot ID Protein )=-Human ACE angiotensin-COnverting enzy-me precursor c'rot -u.lg3 acet an

I

ACHE acetyl Choline receptor protein 3BHI 3-beta hydroxy-S-ene steroid dehydrogenase type I 3BH2 33-beta. hyd=oxy-5-ene steroid dehydrogenase type 11 _1131 41EL 4-IBB ligand BLSA beta-lymphocyte antigen precursor CATD cathepsin D precursor CD69 early activation antigen CD69 CD81 CD81 antigen C002 tumor-associated antigen CO-029 EYt,,,,,hlome P4

IA_

CP11 cytochrome P450 JAj CYRP cytokine receptor common beta-chain precursor CYPC peptidyl-prolyl cis-trans isomerase

C

DIAC di-N-acetylchitobiase precursor DUT deax =idine 51-triphosphate nucleotidohydrolase eo n t a ECP eosinophil cationic protein precursor -at_, ote-_ EV22 ect0trOPic viral integration site 2B protein fo t V2 0 0 al a Pr_ ta. e E E TA alpha fetoprotein r 'in FOL1 folate receptor alpha precursor ta__ T si c1rio HR r ph I a e 'eept al h f- t-p-ot GSHR a-lutathione reductase E ec' op 'l at crl e red IL9 inte 9 n rleukin 9 precursor IN19 interferon-inducible protein 9-27 iNIU tn nte=-feron-inducible protein

I-BU

1NR2 interfezon alpha/beta receptor beta-chain precursor KLTK- leukocyte tyrosine kinase receptor precursor KPCL eta type protein kinase

C

LSP li-popoly-saccharide binding protein precu=sor LCAT lecithin-cholesterol acyltra-msferase EC asialoglycoprotein-recepto= I LFA3 lymphocyte function-associated aftigen-3 precursor.

Lui~M laManin alpha-I chain precursor LONNmitochonrial LON protease homolog precursor~ LONM Mitochondrial LON protease, homolo; precursor.

LYOX -Proteirlaysine 6-oxidase precursor' McA melanoma associated antigen

I.

MAG2 melanoma associated antigen 2 MAC3 melanoma associated antigen 3 MCBR melanocortin-5 receptor XYSE embronic~ myosin heavy chain NOLl proliferating-cell nucleolar antigen P120 NP-MInatural resistance-.associated macrophage Protein 1 NT3 neurotrophin-3 precursor NTCR sodium and chloride -dependent creatin transporter NXSI-NAJA'T cobrotoxin PA_2M anscba n as oI te precu s ph l p 2 r c r o TGLK proenglytanink prten prcra sor~E3pecr T F PI tissue factor pathway inhibitor precursor TRFL lactotransferrin precursor TYK2 non-receptor tyrosine-protein kinase VPRT retrovirus related protease WNT2 WNT-2 protein precusor zinc finger protein The amino acid sequences of nine of the highly conserved epitope regions discussed above are provided below. Three of these regions are on the envelope glycoproteins gpl20 (two targets) and gp41 (one target), one is on the reverse transcriptase heterodimer p66/55, and one is on protease Additional targets are on the Gag precursor with sites on p17 (two targets), p24 and p7.

One epitope region on HIV1SF2 gpl20 extends from amino acid residue 4 through 27 and a second extends from amino acid residue 54 through 76 of HIV1.

Antibodies to epitope regions located on gpl20 function synergistically to effect the release of gpl20 from gp4l. The release of gpl20 from gp41 is antibody dose dependent and can be demonstrated by neutralization assays, such as TCID, which measure HIV infectivity.

An epitope region of a neutralizing or inactivating region of gpl20 of HIV2,, also has been determined. The sequence of HIV 2 ,z envelope glycoprotein gpl20 has been mapped, and from about amino acid residue 7 through 43 is a region mimicking a sequence of HIVls gpl20 and certain human proteins.

Antibody targeting the region results in dissociation of HIV2 gpl20 from gp41, which correlates with a reduction in infectivity.

A third HIV envelope glycoprotein target for HiVls 2 was located at amino acid residues 502-541 of gp4l transmembrane glycoprotein. Antibody targeting of this region in the presence of complement results in an antibody dependent complement mediated lysis of the HIV envelope glycoprotein and marked reduction in HIV infectivity.

In addition to the envelope glycoprotein epitope regions, another HIV1 epitope region of interest includes amino acid residues 254 through 29 5.of the reverse transcriptase heterodimer p66/55. Antibody targeting of this region results in an antibody dose dependent reduction in reverse transcriptase activity.

Also of interest is the epitope region encompassing amino acid residues 69-94 of protease plO. Antibody targeting of this region results in an antibody dose dependent reduction in protease activity.

The targets on reverse transcriptase and protease are in conserved regions adjacent to the enzyme active site, which is well-known for its mutation and subsequent resistance to competitive inhibitors. The antibody-mediated inactivation results from a steric or conformational change in the enzyme with secondary loss of activity. This method of inactivation functions independently and is not influenced by mutation in the enzyme active site and is irreversible.

Also of interest are .three epitope regions within the Gag gene. Specifically, amino acid residues 166 through 181 of Gag gene protein p24, one target at amino acid residues 2 through 23 and a second target at amino acid residues 89 through 122 of Gag gene protein pl7 and amino acid residues 390 through 410 and 438 through 443 of Gag gene protein p7 are useful in this invention. Antibodies targeting these regions result in disruption of the nuclear capsid following lysis of the HIV envelope by the antibodies described above.

This targeting culminates with exposure of HIV RNA to plasma RNAse degradation. Additionally, the targets on pl7 is exposed on the surface of infected lymphocytes following budding. This provides an additional target for ADCC lysis of infected lymphocytes.

One of the specific peptides set forth above, comprising at least one epitope not recognized by antibodies from HIV-infected patients but recognized by goat anti-HIV antibodies, is the peptide comprising amino acid residues 4 through 27 of HIV1 2 envelope protein and linear epitope-containing subsequences thereof, which has the following sequence: KGT RRN Y Q H LWRWGT LL GML M I C This peptide mimics human proteins FOL1, NTCR, PSS1, KLTK, MC5R, ECP, INIU, NI9, VPRT, CD69,

MYSE,

RNKD, ACHE, TCO2, LCAT, MAGI, MAG2, MAG3 and LYOX.

A second epitope-region from the HIV1SF2 envelope glycoprotein extends from amino acid residue 54 through 76, which has the sequence: A S D A R A Y D T E V H N V W A T H A C V P T This peptide mimics proteins CYRB and SYV.

A third epitope region of interest in the envelope of HIVls extends from amino acid residue numbers 502 through 541 of .glycoprotein gp4l. This peptide has the following amino acid sequence: HIVi Env502 R V V Q R 7K R A V G V G A L G F L G A A G S T M G A V S L T L T V Q A R 502-541 502-541 This peptide mimics human proteins CYPC, TYK2,

ACHE,

NTCF, NTCR, CD81, 41BL, NIDO, GSHR, C002 and TCO2.

In another specific embodiment, an epitope region of interest is that of amino acid residues 2 through 23 of the HIV1SF 2 Gag protein pl7. This peptide has the.

sequence: GARAS VLSGGELDRWEKI

RLRP

This peptide mimics human proteins TFPI, PA2M,

BLSA,

ECP, and FETA and certain neurotoxins, such as NXS1 and NAJAT. The peptide has a hydrophobic sequence which binds to and targets host cell membrane and function mimics cellular translation protein Src.

A second target on HIVls p17 extends from amino acid residue 89 through 122. This peptide has the sequence: L Y CV H Q RI D V K D T K E AL E K I EQ N K S K.

This peptide mimics FETA and TRIC.

Another peptide of interest is that of amino acid residues 166 through 181 of the Gag gene protein p24 and epitope containing subsequences therein. This peptide has the sequence: PE VI PM FSALSEGATP This peptide mimics human proteins FETA-and

TRFL.

A third Gag gene protein epitope region of interest is the peptide having amino acid residues 390 through 410 and 438-443 of.Gag gene protein p7 and epitope containing subsequences thereof. This peptide has the sequence: K TV KC FN C GKE GH I AKN CRA K I WS S Q This peptide mimics human FETA and RNA binding proteins. This peptide contains a zinc binding domain which interacts with, and binds to, viral

RNA.

Antibodies to this region enhance the removal of premature HIV devoid of envelope following the lysis of infected CD4+ lymphocytes.

Also of interest as an epitope region is the peptide of amino acid residues 69 through 94 of the protease pl0 and epitope-containing subsequences thereof. This peptide has the sequence: RIG G Q L K E ALL D T GAD D TV LE EM N L P This peptide sequence mimics human proteins RENI,

BLSA,

VPRT and CATD. Antibodies to this sequence inhibit the protease activity of HIV.

A further specific sequence useful in this invention is a sequence encompassing amino acid residues 254 through 295 of HIV1 reverse transcriptase heterodimer p66/55. This peptide has the sequence: G L K K K KS VT VL DV G DA Y F S V P L D K D F R K Y T A F T I P S I N N E T P This peptide sequence mimics human proteins POLl and

ECP.

As noted above, other strains of HIV also can be used to obtain peptides and antibodies in accordance with the present invention. Useful peptides from other strains can be determined by comparing and aligning the sequence of another strain to the sequence of HIV1SF 2 or HIV2N, and finding that part of the sequence homologous to the epitopes of interest identified for HIVIsr 2 or HIV2,,z.

A sequence of interest in HIV2Nz identified by the method of this invention is in the env gpl20 open reading frame and extends from amino acid residue numbers 7 through 43. This peptide has the following sequence: Q L L I A I V L A S A Y L I H C K Q F V T V F Y G I P A W R N A S I P L F This peptide mimics human proteins IL9, SRE1, NRM1, LBP, NOL1, S5A2, LMA1, LECH, LFA3, KPLC, FETA, 3BH2, 3BHI, INR2 and EV2B.

For example, once the desired amino acid sequences have been identified, antibodies which recognize these sequences are obtained. Such antibodies can be obtained using proteins containing the peptides isolated from HIV lysates, synthetic peptides, bacterial fusion proteins and proteins/peptides from phylogenetically unrelated sources which contain the desired epitopes.

If viral lysates are to be used, a protein lysate of a single HIV strain can be used, or a mixture of lysates of two or more different strains can be used.

If a mixture of lysates is used, the mixture can comprise lysates of different HIV1 strains or a combination of at least one HIV1 strain and at least one HIV2 strian. A preferred mixture is a combination of lysates from HIV1sl, HIVIM, and HIV 2 Nz.

Viral lysates initially are treated to remove lipids and other impurities from the HIV proteins. The HIV protein mixture then is treated to remove contaminants of cel culture origin, including human leukocyte antigen (HLA), class I and class II antigens.

Methods for removing these antigens are known in the art and include employing monoclonal anti-HLA class

I

and anti-HLA class II antibodies and immunoaffinity procedures; one method is set forth in detail in Example 3 below.

In addition, it has been found that carbohydates of the HIV proteins must be removed; phylogenically preserved carbohydrates determinants otherwise would stimulate immune responses when the HIV proteins are administered to an animal, resulting in the production of antibodies which would be cytotoxic against human tissues. The proteins are treated with enzymes known to those skilled in the art to remove carbohydrates, including PGNase, neurominidase and glycosidase. One such method is described in detail in Example 3.

The mixture of treated HIV proteins then can be used to immunize an animal to produce antibodies to the peptides of interest. Desirably, the mixture contains approximately equal amounts of the proteins comprising the peptides or epitope regions of interest. That is, desirably they are provided in proportions of approximately 1:1 and the difference in molar ratios between any two peptides is no greater than about 10:1, preferably 3:1.

Alternatively, synthetic peptides can be used as the immunogen. If synthetic peptides are used the amino acid sequence of any desired peptide can be modified by, for example, using a substituted or truncated form of the amino acid sequence.

Amino acid substitutions can be made to avoid predicted enzymatic cleavage that can occur during antigen processing a: a particular amino acid moiety, to force amphipathic conformation to meet required

MHC-

associated antigen presentation and to provide sufficient length for HLA presentation should cleavage occur at or near the epitope boundary. Truncated sequences are selected such that the peptide will retain conformity to epitope length requirements as predicted by MHC class 1 and class 2 antigen presentation motifs. More extensive guidelines on desirable amino acid substitutions are given below as part of the section on synthetic peptides. In addition to substituted and truncated sequences, extended sequences can be prepared in which additional amino acids are added at either end of a selected epitope region for the purpose of facilitating attachment to solid phase supports and macromolecular carriers.

As an example, useful truncated sequences of the peptide extending from amino acid residue 502 through 541 of HIVIF 2 gp41 discussed above include a peptide with the sequence of amino acid residues 512-531: G I V G A M F L G F L G A A G S T M G A and also a sequence extending from amino acid residue 518 through amino acid residue 527: F L G F L G AA G S Another particularly useful truncated peptide is a truncated sequence of the peptide extending from anino acid 7 through 43 of gpl20 of HIV 2 Nz has the following sequence L L I A i V LA S A Y L I H C K Q The peptide can be prepared in a wide variety of ways. The peptide, because of its relatively small size, can be synthesized in solution or on a solid support in accordance with conventional techniques.

Various automatic synthesizers are commercially available today and can be used in accordance with known protocols. See, for example, Stewart and Young, Solid Phase Pepride Synthesis, 2nd ed., Pierce Chemical Co., 1984; and Tam et al., J. Am Chem. Soc. (1983) 105:6442.

Alternatively, hybrid DNA technology can be employed where a synthetic gene is prepared by employing single strands which code for the polypeptide or substantially complementary strands thereof, where the single strands overlap and can be brought together in an annealing medium so as to hybridize. The hybridized strands then can be ligated to form the complete gene, and, by choice of appropriate termini, the gene can be inserted into an expression vector, many of which are readily available today. See, for example, Maniatis et al., Molecular Cloning,

A

Laboratory Manual, CSH, Cold Spring Harbor Laboratory,.

1982. Or, the region of the viral genome coding for the peptide can be cloned by conventional recombinant DNA techniques and expressed in procaryotic or eukaryotic expression systems to produce the desired peptides.

Preferably, the immunogen will be enriched for the desired epitopes to which antibody-producing

B

lymphocytes will respond by producing antibodies that will neutralize and inactivate essential steps in the life cycle of HIV infection. As used herein, "enriched" means that a desired epitope constitutes at least 25% of the HIV protein, preferably at least and most preferably about 95%. More particularly, solutibns containing disrupted virus lysate or extracts, or supernatant of biologically-expressed recombinant proteins or disrupted expression vectors or proteins containing mimicked epitopes can be enriched for such proteins, as desired, using purification methods, such as, for example, polyacrylamide gel electrophoresis. Immunoaffinity purification is a preferred and convenient method for purification of proteins and peptides containing the desired

HIV

epitopes, affinity purification using mono specific affinity purified polyclonal or monoclonal antibodies. The extent to which the peptides are purified from the solutions for use as an immunogen can vary widely, from about 50%, typically at least to 95%, desirably 95% to 99% and, most desirably, to absolute homogene.

To obtain antibodies to the desired epitopes, animals are immunized with either the peptides of interest or HIV proteins containing them which have been treated to remove carbohydrates. and HLA antigens as disclosed above. Immunization protocols are well.

known and can vary considerably yet remain effective.

See Colco, Current Protocols in Immunology, John Wiley.

and Sons, Inc. 1995. The proteins and/or peptides can be suspended or diluted in an appropriate physiological carrier for immunization. Suitable carriers are any biologically compatible, non-toxic substance to deliver and/or enhance the immunogenicity of the peptides, including sterile water and 0.9% saline.

Alternatively, the peptides can be coupled to a carrier molecule before being used as an immunogen.

One preferred technique, for example, discussed in more detail below, involves the attachment of the proteins and fragments thereof to multiple repeats of a glcopeptide, such as muramyl dipeptide (MDP), to form a microparticle, typically less than 1 micron, and preferably less than 0.2 microns, in diameter. The microparticle then can be dispersed in a pharmaceutical carrier for injection. This procedure achieves a high density of the peptide which can be used to elicit the desired immune response. The selection of carrier will vary depending upon the route of administration and response. The compositions can be sterilized by conventional, well-known sterilization techniques. The pepzides can be administered by oral or parenteral routes, preferably the latter.

Immunogenic amounts of antigenic preparations enriched for the desired epitopes are injected, generally at concentrations in the range of 1 pg to mg/kg body weight of host. Administration can be by injection, intramuscularly, peritoneally, subcutaneously, intravenously, etc. Administration can be one time or a plurality of times, usually at one to four week intervals.

Immunized animals are monitored for production o.f antibody to the desired epitope. High affinity complement fixing IgG antibody is preferred for passive immunotherapy and can be used intact or as fragments such as Fv, Fab, F(ab') 2 Antibody fragments may be preferable when greater tissue penetration is desirable. Antibodies and fragments can be given alone or as conjugates with toxic substances or isotopes.

Once the desired antibody response is attained, blood is collected by, for example, venipuncture, cardiac puncture, or plasmapheresis. Antibodies are purified from the complex serum or plasma mixture in accordance with conventional procedures, including, for example, salt precipitation, ion exchange chromatography, size chromatography, affinity chromatography. Oftentimes, a combination of methods is used. *Immunoaffinity chromatography is a preferred method.

To circumvent possible antigenicity in a human receiving antibody derived from a non-human animal, recombinant antibodies can be constructed. One type of recombinant antibody is a chimeric antibody, wherein the antigen binding fragment of an immunoglobulin molecule (variable region) is connected by a peptide linkage to at least part of another protein not recognized as foreign by humans, such as the constant portion of a human immunoglobulin molecule. This can be accomplished by fusing the animal variable region exons with human kappa or gamma constant region exons.

Various techniques are known to the skilled artisan, such as those described in ?CT 86/01533, EP171496, and EP173494, the disclosures of which are incorporated herein by reference. A preferred type of recombinant antibodies is CDR-grafted antibodies.

PHARMACEUTICAL FORMULATIONS AND THEIR USE The antibodies of this invention that neutralize infectivity, kill infected CD4 lymphocytes and inactivate functionally important events in the life cycle of HIV are incorporated as components of pharmaceutical compositions. The compositions comprise a therapeutic or prophylactic amount of at least one of the antibodies of this invention, and desirably an antibody cocktail, with a pharmaceutically acceptable carrier. A pharmaceutically acceptable carrier is any compatible, non-toxic substance suitable for the delivery of the antibodies to the patient. Thus, this invention provides compositions for parenteral administration which comprise a solution of antibody dissolved in an acceptable carrier, preferably an aqueous carrier. A variety of aqueous carriers can be used, water, buffered water, 0.4% saline, 0.9% saline, 0.3% glycine and the like. These solutions are sterile and generally free of particulate matter. The compositions further can comprise pharmaceutically acceptable auxiliary substances as required to approximate physiological conditions, such as pH adjusting and buffering agents, toxicity adjusting agents and the like. For example, sodium acetate, sodium chloride, potassium chloride, calcium chloride, sodium lactate, etc., can be used. The concentration of antibody in these formulations can vary, typically from less than about 0.1 mg/ml to as much as 150 or 200 mg/ml, preferably between about 1 mg/ml and about mg/ml, and will be selected primarily based on fluid volumes, viscosities, etc., preferably for the particular mode of administration selected.

Determining the concentration of a particular antibody or antibody cocktail is within the abilities of one of ordinary skill in the art. Thus, a typical pharmaceutical composition for intravenous infusion can be made up to contain 250 ml of sterile Ringer's solution and 100-200 mg of antibody. Compositions for intramuscular injection can be made up to contain 1 ml sterile buffered water and about 20 to about 50 mg of antibody.- Actual methods for preparing parenterally administrable compositions will be known or apparent to those skilled in the art and are described in more detail in, for example, Remington's Pharmaceutical Science, 15th Ed., Mack Publishing Company, Easton, Pa.

(1980), which is incorporated herein by reference.

Such compositions can contain a single antibody which is, for example, specific for certain strains of HIV or for a single protein or glycoprotein expressed by most and, more preferably, all strains of HIV.

Alternatively, a pharmaceutical composition can contain more than one antibody to form a "cocktail." For example, a cocktail containing antibodies against various proteins and strains of HIV would be a universal product with therapeutic or prophylactic activity against the great majority of the clinical isolates of HIV. The cock-ail can contain antibodies which bind to epitopes on proteins or glycoproteins of the HIV envelope, for example, or can contain a combination of antibodies to epitope sites identified above on HIVlSF2 Env proteins gpl60, gpl20, and gp41; Gag protein p7, p17 and p24; reverse transcriptase heterodimer p66/55 and protease pl0, or a subgroup thereof, thus neutralizing a series of epitopes crucial in the life cycle of HIV. Antibodies to epitope sites on other neutralizing or inactivating regions of HIV proteins also, of course, can be employed.

For example, antibodies which modify attachment, cell entry, transcription, translation, assembly, targeting of the mature virion to the plasma membrane and extrusion of the virion will interfere with HIV life cycle events. Antibody cocktails will more frequently be employed to obtain inactivation of multiple essential HIV proteins. This will be of therapeutic benefit in particular within virions lack the outer envelope but possibly are infectious should they gain cell entry by other mechanisms such as micropinocytosis or transfection or the like. The molar ratio of the various antibody components usually will not differ by more than a factor of 10, more usually by not more than a factor of 5, and will usually be in a molar ratio of about 1:1-3 to each of the other antibody components.

With respect to antibodies to the nine specific peptides set forth above, a desirable antibody cocktail comprises antibodies to the two envelope gpl20 peptides and gp41 peptide. More desirably, the cocktail comprises antibodies to those three epitope regions plus an antibody to the protease pl0 epitope region.

Even more desirably, the cocktail comprises antibodies to those four epitope regions plus antibodies to at least one of the other five enumerated epitope regions.

in a most preferred embodiment, the cocktail comprises antibodies to all nine of the epitope regions.

The antibodies and antibody cocktails of the present invention can be administered independently or given in conjunction with other anti-retroviral agents. The current status of the development of other anti-retroviral agents, and of anti-HIV agents in particular, is reviewed in Mitsuya et al., Nature 325:773-778, 1987.

The antibodies and peptides of this invention can be stored in liquid format at various temperatures known to preserve antibody activity, e.g. -70 0 c, -20°C, and 0-4 0 C or lyophilized for storage and reconstituted in a suitable carrier prior to use. This technique has been shown to be effective with conventional immune globulins, purified antibodies, and immunogens composed of proteins, glycoproteins, and peptides. Art-known lyophilization and reconstitution techniques can be employed and it will be appreciated by those skilled in the art that lyophilization and reconstitution can lead to varying degrees of antibody activity loss wit h conventional immune globulins, IgM antibodies tend to have gre ater activity loss than IgG antibodies) and that doses may have to be adjusted to compensate for any loss.

The compositions containing the present antibodies or cocktails thereof can be administered for the therapeutic and/or prophylactic treatment of HIV infections. In therapeutic application, Compositions are administered to a patient already infected with H-IV, in an amount sufficient to treat or at least partially arrest the infection and its complications.

An amount adequate to accomplish this is defined as a "therapeutically effective dose." Amounts effective for this use will depend upon the severity of the infection and the general state ofl the patient's own immune system, but generally range from about 0.1 to about 200 mg ofL antibody per kilogram of body weight with dosages of from 0.5 to 25 mg per kilogram being preferred. The compositions of this invention can be employed in serious disease states that are ILife-threatening or potentially life-threatening situations. in such cases, it is possible and may be felt desirable by the treating physician to administer substantial excesses of these antibodies.

in prophylactic applications, compositions containing the present antibodies or a cocktail thereof are administered to a patient not already infected by HIV, but perhaps recently exposed to or thought to have been exposed to, or at risk of being exposed to the virus .(such as, for example, the newborn of an HI.V infected individual), or immediately following an exposure or suspected exposure to HIV. If the composition is to be administered to an HIV-infected pregnant female, it can be given once or multiple times prior to delivery to reduce HIV infectivity in maternal blood and thereby reduce the risk of HIV transmission to the.newborn. The newborn at risk also can be treated to further reduce the risk of contracting

HIV.

An amount defined to be a "prophylactically effective dose" generally ranges from 0.1 mg to 25 mg per kilogram of body weight, depending upon the patient's state of health and general level of immunity.

In addition, the antibodies of the present invention can find use as a target-specific carrier molecule. An antibody can be bound to a toxin to form an immunotoxin or a radioactive material or drug to form a radiopharmaceutical or pharmaceutical. Methods for producing immunotoxins and radiopharmaceuticals are well known (see, for example, Cancer Treatment Reports 68:317 (1984)). Heteroaggregates of antibodies of the present invention and human T-cell activators, such as monoclonal antibodies to the CD3 antigen or to the Fc gamma receptor on T-cells, can enable human T-cells or Fc-gamma bearing cells (such as K cells or neutrophils) to kill HIV infected cells via antibody dependent cell-mediated cytolysis (ADCC). Such heteroaggregates can be assembled, for example, by covalently cross-linking the anti-HIV antibodies to the anti-CD3 antibodies using the heterobifunctional reagent N-succinimidyl-3-( 2 -pyridyl dithiol)propionate, as described in Karpowsky et al., J. Exp. Med. 160:1686 (1984), which is incorporated by reference herein.

Other anti-HIV agents also can be included in the formulations such as 3'-azido-3'-deoxythymidine, 2',3'-dideoxycytidine, 2',3'-dideoxy-2', 3'-didehydrocytidine, etc.

In addition to antibody compositions, compositions comprising the peptides of this invnetion can be administered for therapeutic and prophylactic vaccination of HIV-infected individuals. For therapeutic application, compositions comprising peptides, either as isolated peptides optionally modified as discussed above or contained within HIV proteins treated as described above and desirably coupled to an MDP microparticle to further stimulate immunogenicity, are administered to a patient infected with HIV. The amount of peptide administered is chosen so as to stimulate antibody production to functional HIV epitopes not previously recognized by the patient's immune system so that the stimulated antibodies can arrest the infection. in prophylactic applications, compositions of the peptides coupled to the microparticle MDP are administered to persons not infected with HIV to stimulate the production of antibodies against otherwise unrecognized epitopes to provide a protective function against future infection.

DIAGNOSTIC AND PROGNOSTIC

USES

OF ANTIBODIES AND ANTIGEN The antibodies and epitopes recognized by them and disclosed in the present invention also are useful for the diagnosis and management of HIV infection.

Typically, diagnostic assays employing antibodies and/or their respective antigens entail the detection of the antigen-antibody complex. Numerous immunoassay configurations have been described and employ either labeled or unlabeled immunochemicals for this purpose.

When unlabeled, the antibodies find use, for example, in agglutination assays, antibody dependent complement mediated cytolysis assays, and neutralization assays.

Unlabeled antibodies can be used in combination with other, labeled, antibodies (second antibodies) that are reactive with the primary antibody, such as antibodies specific for immunoglobulin; Unlabeled antibodies can be used in combination with a labeled antibody which is reactive with a non-competitive epitope on the same antigen, such as in sandwich type immunoassays, or in combination with a labeled antigen. Alternatively, the antibodies can be directly labeled and used in both competitive and non-competitive immunoassays. These assay types and configurations are well known in the art. A wide variety of labels can be employed, such as radioisotopes, fluorescent tags, enzymes, enzyme substrates, enzyme cofactors, enzyme inhibitors, ligands (particularly haptens), etc. Numerous types of immunoassays are available and, by way of example, include those described in U.S. Pat. Nos. 3,817,827; 3,850,752; 3,901,654; 3,935,074; 3,984,533; 3,996,345; 4,034,074; and 4,098,876.

Commonly, the antibodies and peptides of the present invention are utilized in enzyme immunoassays, where, for example, the subject antibodies, or their respective antigens are conjugated to an enzyme and the immunoassay is configured to provide maximal sensitivity and specificity in detecting HIV antigens in biological samples such as human blood serum, saliva, semen, vaginal secretions or viral infected cell culture suspension.

Kits also can be designed for use with the subject antibodies for use in the detection of HIV infection or the presence of HIV antigen. The kits comprise antibodies of the present invention optionally in conjunction with additional antibodies specific for other epitopes of HIV. The antibodies, which can be conjugated to a label, unconjugated or bound to a solid support such as the surface of a microtiter plate well or a polystyrene bead, are included in the kits with buffers, such as Tris, phosphate, carbonate, etc., stabilizers, biocides, inert proteins, bovine serum albumin, or the like. Generally, these materials will be present in less than about 5% wt. based on the amount of active antibody, and usually present in total amount of at least about 0.001% wt. based again on the antibody concentration. Frequently, it will be desirable to include an inert extender or excipient to dilute the active ingredients, where the excipient can be present in from about 1% to 99% wt. of the total composition. Where a second antibody capable of binding to the antibody is employed, the second antibody usually will be present in a separate vial.

The second antibody typically is conjugated to a label and formulated in an analogous manner with the antibody formulations described above. The subject epitope recognized by the antibody can be provided labeled or non-labeled and can be provided as part of a larger protein (synthetic, recombinant, or native), with or without modification such as the addition of spacer arms, amino groups, or cysteine residues, which can be used to attach the peptide to a support and extend it from the surface of the support. Such modifications are employed to provide the epitope in an arrangement to optimize immunoreactivity with the antibody. Such peptides are formulated in a manner analogous to that of the epitope-containing proteins as described above.

The detection of HIV. antigens, or the whole virus, in various biological samples is useful in diagnosing a current infection by HIV, evaluating response to therapy, enumerating infected cells, serotyping HIV strains (clades), identifying and quantitating virulence factors associated with primary infection, progression and complications such as peripheral neuropathy, multi focal leukoencephalopathy, and Kaposi's sarcoma. Biological samples can include, but are not limited to, blood, serum, saliva, semen, tissue biopsy samples (brain, skin, lymph nodes, spleen, etc.), cell culture supernatants, disrupted eukaryotic and bacterial expression systems, and the like. Presence of virus, viral antigens, virulence factors, and serotyping determinants are tested for by incubating the antibody with the biological sample under conditions conducive to immune complex formation, followed by the detection of complex formation. In one embodiment, complex formation is detected through use of a second antibody capable of binding to the primary antibody and typically conjugated to a label.

The second antibody is formulated in a manner analogous to that described for the primary antibody formulations described above. In another embodiment, the antibody is attached to a solid phase support which then is contacted with a biological sample. Following an incubation step, labeled antibody is added to detect the bound antigen. In another embodiment, the antibody is conjugated to a detection label and following an incubation step with a biological sample, such as cells or tissue sections, the sample is evaluated by flow cytometry or microscopy for the antigen.

PREPARATION AND USE OF SYNTHETIC

PEPTIDES

Peptides of this invention can be modified by introducing amino acid substitutions into the peptide.

Substitutions may be desirable to vary one or more particular amino acids to more effectively mimic the epitopes of the different retroviral strains or to enhance immunological responses or MHC interactions with the epitope resulting in greater immunogenicity of the mimicked epitope when used for immunization or vaccination. In addition, it can be desirable to make certain amino acid substitutions to enhance the chemical stability of the peptide.

More specifically, a polypeptide employed in the subject invention need not be identical to any particular HIV polypeptide sequence, so long as it is able to provide immunological competition with proteins of at least one of the strains of HIV. Therefore, the subject polypeptides can be subject to various changes, such as insertions, deletions, and substitutions, either conservative or -non-conservative, where such changes will enhance the desired activity of the peptide. Conservative substitutions are substitutions with similar amino acids within a group such as neutral, acidic, basic, and hydrophobic amino acids.

Examples of substitutions within such groups would include gly, ala; val, ile, leu; asp, glu; asn, gln; ser, thr; lys, arg; phe, tyr; and nor, met. Additional amino acid substitutions, obtained by application of molecular modelling software to HLA allotyping database classification (DNA and serological), are shown: Group, 1 letter code Group, 3-letter code F,S phe, ser Q,R g1n, arg K, N lys, asn E.G glu, gly G,R gly, arg H,Q his, gln I, T ile, thr V, A val, ala D,N asp, asn Y,F tyr, phe I, F ile, phe K,E lys, glu E,L,R glu,leu,arg Y,L tyr, leu

S,V

Y, N, F, D

D,R

L, F ser, val tyr, asn, phe, asp asp, arg leu, phe In a preferred embodiment of the invention, amino acid modifications are made so as to substitute hydrophilic residues on the more hydrophilic end of the peptide of interest and hydrophobic residues on the more hydrophobic end of the peptide. Such substitutions result in the formation of an amphipathic helix with the desired epitope bracketed between the substitutions. Substituted amino acids in the D isomer can be employed to bracket epitopes to protect and stabilize the epitope and enhance immunogenicity of the epitope. Since D-amino acids are not cleaved by intracellular enzymes, such protion provides peptide epitopes of the desired length for interaction with MHC molecules when they are inserted at appropriate sites.

This is described in detail in Example 8.5 below.

Usually, the modified sequence will not differ by more than about 20% from the sequence of at least one strain of the human immunodeficiency retrovirus except where additional amino acids are added at one or both termini for the purpose of providing an "arm" by which.

the peptide of this invention conveniently can be immobilized on solid phase supports, attached to macro molecules or modified to enhance immunogenicity by altering or enhancing MHC binding and presentation.

The arms can comprise a single amino acid or as many as 50 or more amino acids, and typically are 1 to 10 amino acids, in length.

Amino acids such as tyrosine, cysteine, lysine, glutamic or aspartic acid, or the like can be introduced at the C-or N-terminus of the peptide or oligopeptide to provide for a useful functionality for linking. Cysteine is particularly preferred to facilitate covalent coupling to other peptides or to form polymers by oxidation.

Additionally, the peptide or oligopeptide sequences can differ from the natural sequence by the sequence being modified by terminal-

NH

2 acylation acetylation), thioglycolic acid amidation, or terminal carboxy amidation with ammonia or methylamine)-, to provide stability, increased hydrophobicity for linking or binding to a support or other molecule, or for polymerization.

Thus, for example, in a preferred embodiment of the peptides disclosed herein, one or more cysteine residues or a combination of one or more cysteine residues with spacer amino acids can be added to the termini of the peptide. Glycine is a particularly preferred spacer when individual peptides are desired.

When multiple peptide repeats of the peptide are desired, the peptide is synthesized off of a lysine core to form a tetravalent peptide repeat.. The configuration is shown by way of example. Preferred peptides for use in oxidative polymerization are those in which at least two cysteine residues are added to the termini of a desired peptide. When two cysteine residues are present at the same end of the peptide, a preferred embodiment exists when the cysteine residues are separated by one to three spacer amino acid residues, preferably glycine. The presence of cysteine residues may allow the formation of dimers of the peptide and/or increase the hydrophobicity .of the resulting peptide which facilitates immobilization of the peptide in solid phase or immobilized assay systems. Of particular interest is the use of the mercapto group of cysteines or thioglycolic acids used for acylating terminal amino groups or as the first amino acid for building multiple peptide repeats or the like for linking two of the peptides or oligopeptides or combinations thereof by a disulfide linkage or a longer linkage to form polymers that contain a number of epitopes. Such polymers have the advantage of increased immunological reaction. Where different peptides are desired for immunization, they are individually assembled and combined in a cocktail to provide the additional ability to induce antibodies that immunoreact with several antigenic determinants of different HIV isolates. To achieve the formation of antigenic polymers (synthetic multimers), compounds can be employed having bis-haloacetyl groups, nitroarylhalides, or the like, where the reagents are specific for these groups. The linking between the one or two mercapto groups of the peptides or oligopeptides can be a single bond or a linking.group of at least 2 or more carbon atoms.

LINKING PEPTIDES TO MACROMOLECULAR

CARRIERS

The subject peptide can be employed linked to a soluble macromolecular not less than 5 kDal) carrier. Conveniently, the carrier can be a poly(amino acid), either naturally occurring or synthetic, to which antibodies are unlikely to be encountered in human serum. Examples of such carriers are poly-L-lysine, keyhole limpet hemocyanin, thyroglobulin, albumins, such as bovine serum albumin, tetanus toxoid, etc. The choice of the carrier is primarily dependent upon the ultimate use intended for the antigen and one of convenience and availability.

In a preferred embodiment, the carrier comprises multiple repeats of glycopeptide a microparticle which can be synthesized or isolated from certain bacteria such as Proprionibacterium acini or the like. This microparticle is composed of muramyl dipeptide extensively crosslinked resulting in muitimeric configurations.

When muramyl dipeptide is isolated -from Propionibacterium acini or related organisms, strain selection is helpful, and selection is based on chemical analysis of the bacterial cell wall. The preferred embodiment is muramyl dipeptide extensively crosslinked with a dipeptide composed of L-alanine-Disoglutamine.

From preliminary experiments, strain differences have been identified in which dipeptide composition and peptide length vary. Isolates with high concentrations of lipid A and 0-acylated beta myristate are components of the cell wall. Preliminary experiments showed these differences are associated with increases in toxicity and decreases in adjuvant effect. Strain selection and the purification of the preferred embodiment is discussed by way of Example 4, below.

The MDP microparticle can be synthesized by employing procedures known in the art. It has been well established that MDP is a potent immunostimulant but has significant toxicity. Many attempts to reduce MDP toxicity have employed procedures to delay release, such as MDP incorporation into liposomes or other related compounds or modification of terminal groups.

Chemical modification resulted in marked reduction in the desired adjuvant effect, and designs which change delivery rate have been difficult to control. By way of example, MDP microparticle configuration, size parameters, and antigen delivery attachment methods are provided below. Removal of lipids from the microparticle configuration facilitates rapid internalization of MDP by antigen presenting cells (APC). Antigen presenting cells are predominately of monocytes lineage and include monocytes, macrophage, Histiocytes, Kuffer cells, Dendritic cells, Langerhans cells, etc. and participate in antigen processing and antigen presentation through MHC associated events.

Factors which contribute to the development of immune responses to foreign protein can be, in part, determined by amino acid sequence and sequence susceptible to protease cleavage in the micro environment. Successful immune responses are most frequently observed to peptides which form an amphipathic helix with a hydrophobic terminus, preferably on the amino terminal end, and hydrophilic amino acids most frequently on the carboxyl terminal end. Sequence configurations that are resistant to protease degradation and form amphipathic helix arrangements are frequently strong immunogens. Residues which contain proline in the sequence are generally poorly immunogenic by preventing helix formation and glycosylation sites are less favorable and frequently inhibit responses directed at peptide epitopes.

Antigen challenge which results in a successful immunological response in the host animal requires antigen processing and presentation of antigen through MHC associated events. Exogenous antigen is primarily.

processed by antigen presenting cells (APC) after internalization into endosomes. Following proteolysis by enzymes, such as cathepsin D, which are present and react in this acid environment, peptide fragments which satisfy the criteria described above are assembled with MHC class II and presented on the cell surface. When peptides are presented in sufficient density immune events result. The type of immune response is driven by the density of peptide per APC, micro-environment, the cytokine environment, and the lymphocyte type initially stimulated by antigen presenting cells.

Following internalization, a cascade of -cytokine responses is induced which modifies the microenvironment and establishes conditions conducive of immunological events.

By way of example, a unique MDP microparticle (0.01 0.2 micron) is used to deliver immunogen to antigen presenting cells resulting in immune responses to poorly immunogenic epitopes not observed using conventional methods as shown in Example 5 below.

Quantitation of these immune responses demonstrate to 100 fold increases in antibody concentration as compared to other adjuvants.

Subject peptides employed as immunogen can be linked to the carboxyl terminal amino acid moiety of muramyl dipeptide using either the amino or carboxyl terminus of the subject peptide or to the aldehyde oxidation product of the carbohydrate moiety as disclosed in the examples. There will be at least one molecule of the subject peptide per MDP microparticle, preferably 10-100 molecules of subject peptide per MDP microparticle and most preferably 100 to 1000 subject peptides per MDP microparticle. Carrier size and available linkage groups, therefore, will influence the number of subject peptides per carrier.

Macro-carrier composition affects immunogenicity by influencing preferential cell uptake, peptide halflife, and antigen presentation through

MHC

immunological events. One or more different subject peptides can be linked to the same macro-carrier but preferably a single subject peptide is attached either in the univalent or tetravalent configuration to the macro-carrier. When immunization with more than one subject peptide is desired, a cocktail of subject peptide macro-carrier conjugates can be prepared by mixing individual conjugates at ratios to optimize immunogenicity of each subject peptide introduced in the cocktail. in this configuration sufficient peptide is available on each macro-carrier conjugate (100-1000 peptides) to enhance antigen presentation by a single antigen presenting cell. Immunogenicity of the subject peptide will be optimized by adjusting both the number of subject peptides per macromolecular carrier, presentation configuration, such as amino versus carboxyl attachment, terminal amino acid modification, and space arm length and composition, as disclosed.

In this configuration, antigen processing by the antigen presenting cell results in a high density, usually more than 100 and most frequently more than 500 peptides, presented at the cell surface of the antigen presenting cell through MHC interactions. With this configuration significantly higher concentrations of antibody are produced, following immunization, as shown in the examples below.

The manner of linking is conventional, employing such reagents as p-maleimidobenzoic acid, p-methyldithiobenzoic acid, maleic acid anhydride, succinic acid anhydride, glutaraldehyde, etc. The linkage can be made at the N-terminus, C-terminus, or at a site intermediate to the ends of the molecule.

With multiple repeats of muramyl dipeptide, attachment of the subject peptide to aldehyde groups produced by the mild oxidation of sugar residues with, for example, sodium periodate following mild reduction with sodium borohydride and the like, the Schiff's base intermediate is converted to a stable covalent linkage.

The number of peptides per microparticle can be controlled by varying oxidation conditions and quantitated by employing a radioactive tracer. These methods are well known in the art. The method of attachment and attachment configuration can vary from peptide to peptide as needed to achieve the desired response.

Various assay protocols familiar to those skilled in the art can be employed for detecting the presence of either antibodies to retroviral protein epitopes or detecting retroviral proteins in complex protein mixtures. Of particular interest is a novel assay herein disclosed in which the subject peptide is covalently attached to a detection label such as horseradish peroxidase and the native HIV protein expressing epitope or epitopes is either directly or indirectly attached to a solid phase support. In this configuration an antibody which recognizes the peptide epitope will bridge the epitope on the solid phase with the epitope on the label. With this method the epitope reactivity of an antibody to HIV can be determined and quantitated by varying the peptides attached to the label. Peptide epitopes which are associated with HIV serotype, virulence factors or other HIV characteristics can be identified and measured in any sample expressing these epitopes by.a one step competitive immunoassay herein disclosed and provided by way of example.

Use of Antibodies and Their Resective Epitones In Immunoaffinitv Purification Procedures Antibodies specific for epitopes contained within HIV proteins and purified proteins containing these epitopes are of particular advantage for use in immunoaffinity purification of proteins and peptides containing these epitopes and antibodies reactive with them. Generally, the antibodies will have affinity association constants on the order of 108 to 1012 M.

Such antibodies can be used to purify proteins and peptides containing the epitopes of interest.

Oftentimes, genetically modified bacteria can be used to make HIV proteins, and the recombinant fusion proteins of interest can be purified from the culture medium of the recombinant expression system if the expressed protein is secreted, or from the components 2= of the disrupted biological expression system if it is not secreted, or from complex biological mixtures of proteins of which some or one component contains the epitope mimicking an epitope on HIV. Generally, the antibodies which are capable of reacting with HIV epitopes are attached to or immobilized on a substrate or support. The solution containing the epitopes then is contacted with the immobilized antibody under conditions suitable for the formation of immune complexes between the antibody and the protein containing the epitope. Unbound material is separated from the bound immune complexes, and the bound proteins are released from the immobilized antibody and recovered in the eluate.

Similarly, proteins or peptides containing epitopes of HIV or mimicking epitopes of HIV can be attached to or immobilized on a substrate or support and used to isolate antibodies of interest from a solution. A solution containing the antibodies, such as plasma from which albumin has been removed, is passed through a column of immobilized peptides or proteins containing the desired epitopes and, following immune complex formation, non-reactive antibody is separated from the bound immune complex and the antibody is released with an elution buffer and recovered in the eluate. This is of particular value in purifying protein containing epitopes mimicking epitopes of HIV but derived from sources phylogenetically unrelated to HIV.

Typically, antibodies are crudely purified from hyperimmune sera. Ascites fluid or cell culture supernatants and proteins or peptides containing epitopes mimicking HIV epitopes will be crudely purified from biological sources such as, but not limited to, body fluids, blood, blood components, cell extracts, tissue extracts of both adult and embryonic origins, and culture supernatants, extracts of cultured cells, venoms, and recombinant fusion products prior to attachment to a support. Such procedures are well known by those skilled in the art and may include fractionation with neutral salts at high concentration.

Other purification methods, such as ion exchange chromatography, gel filtration chromatography, preparative gel electrophoresis, or affinity chromatography, also can be used to increase the purity of the preparation prior to its use as an immunoabsorbant. Affinity purified antibody can be prepared when desired by reacting crudely purified antibody preparations with a support matrix to which the reactive epitope or protein containing the epitope has been attached.

Of particular interest are antibodies to HIV epitopes phylogenetically mimicked through nature.

Such antibodies are useful as therapeutic agents and also are useful for studying HIV by allowing purification of HIV proteins and the mapping of HIV proteins for sequence location and function. Such antibodies can be produced by immunization with HIV proteins/peptides derived from HIV and immunoaffinity purified by reacting the resultant hyperimmune polyclonal multivalent antisera with proteins/peptides derived from non-HIV sources such as embryonic proteins, venoms, and non-HIV microbial/viral components immobilized on a support as discussed above.

The resulting immunoaffinity purified antibody is epitope specific for an epitope or epitopes shared by HIV and the phylogenetically unrelated protein used for its immunopurification. These epitope specific antibodies have particular utility in the immunoaffinity purification of proteins and peptides of both HIV and non-HIV origin. Such antibodies can be used to map the location of the epitope on HIV to determine its sequence, evaluate functional importance in the life cycle of HIV, its distribution within the clades of HIV and among other retroviridae, its association with

HIV

virulence, and, when non-toxic to man but neutralizing a crucial function in the life cycle of HIV, used to treat HIV infection.

The support to which the antibodies or epitopes are immobilized desirably has the following general characteristics: weak interactions with proteins in general to minimize non-specific binding, good flow characteristics which allow the flow through of high molecular weight materials, possession of chemical groups that can be activated or modified to allow chemical linkage of the antibody or epitope, (d) physical and chemical stability in the conditions used.

to link the antibody, and stability to the conditions and constituents of the buffers required for absorption and elution of the antigen. Some supports commonly used are agarose, derivatized polystyrenes, polysaccharides, polyacrylamide beads, activated cellulose, glass and the like. Various chemical methods exist for the attachment of antibodies and antigens to substrate supports. See -generally, Cuatrecasas, Advances in Enzymology 36:29 (1972).

The antibodies and antigens of the present invention can be attached directly to the support or, alternatively, through a linker or spacer arm. General conditions required for immobilization of antibody and antigens to chromatographic supports are well known in the art. See, for example, Tijssen, 1985, Practice and Theory of Enzyme Immunoassay, which is incorporated herein by reference. Actual coupling procedures will depend slightly on the characteristics and type of the antibody or the antigen to be coupled.

Attachment typically occurs through covalent bonds.

An immune serum, ascites fluid or culture supernatant rich in antibody or extract or lysate of HIV virus, the supernatant or extract from a cultured biological expression system, the supernatant or extract from a suspension of the disrupted cells tissue or blood component (adult and embryonic) or other complex protein mixtures such as venoms, body fluids, or culture products containing the epitope then is added to the appropriate separation matrix. The mixture is incubated under conditions and for a time sufficient for antigen-antibody attachment to occur, usually at least 30 minutes, more usually 2 to 24 hours. The immobilized immune complexes containing the specifically bound antibody or epitopes then are separated from the complex mixture and extensively washed with absorption buffer to remove non-bound contaminants. The immune complexes then can be dissociated with an elution buffer compatible with the particular support, the attached protein, and the eluate protein. The elutable protein, antigen, or antibody is recovered in the eluate. Elution buffers and techniques are well known by those skilled in the art. Peptides that contain the epitope recognized by the antibody can be used in the elution buffer to compete for the antibody binding site and elutions can be performed under mild elution conditions. The selectively absorbed protein can be eluted from the affinity absorbent by altering the pH and/or ionic strength of the buffer or with chaotropic agents. The selection of an elution buffer, its concentration and other eluting conditions are dependent on the characteristics of the antibody-antigen interaction, and once determined should not be subject to significant change.

The eluted protein may require adjustment to a physiologic pH and ionic strength if low or. high pH or ionic strength buffers or chaotrophic agents are used to dissociate the immune complex. Such adjustment can be made by dialysis or gel filtration chromatography.

These methods also permit the eluted protein to regain its native conformation.

The foregoing methods yield, substantially purified proteins containing epitopes of, or mimicking epitopes of, HIV and antibodies reactive with the epitopes. The purified proteins typically will be greater than 50% pure, more usually at least 75% pure, and frequently greater than 95% to 99% pure.

Other features and advantages of the present invention will become apparent from the following experimental descriptions, which describe the invention by way of example. The examples further illustrate the process. of this invention but are not meant to limit the invention in any way.

Preparation of Human Anti-HIV Antibody (IgG fraction) Pools For Use In Mapping HIV Epitope Differences Between Man and Goat Immune Reactions Human sera from HIV-infected patients were obtained from a community health clinic with patient permission, patient signed informed consent, physician approval and local IRB approval. All sera were initially screened for HIV reactivity at a dilution of employing a commercially available test kit from Abbott Laboratories. Sera with high reactivity (arbitrarily defined by test result absorabances greater than 1) were further evaluated by Western Blot analysis to identify those sera with antibody reactivities- to most HIV proteins (env, gag and pol).

Patient sera that demonstrated good reactivity to most HIV proteins as defined were further evaluated by microculture neutralization assays to identify the sera-containing antibody specifities which would neutralize HIV infectivity in microculture assays (total culturable infectious dose (TCID) neutralization). Twenty-nine patients' sera with high antibody titer reactivity to gpl60, gpl20, p66/55, gp41, p 24 and p17 and p10, and neutralized

HIV

infectivity in microculture were identified and pooled (20-40 ml each). Further evaluation of the pooled anti-human HIV revealed broad neutralization activity against multiple strains of HIV and high titer antibody (positive by Western Blot 1:100 or greater) to the nine epitope regions described in detail above. Human IgG.

was purified from this serum pool employing conventional procedures. Following purification the total IgG concentration was adjusted to 10 mg/ml, its composition and purity was evaluated by standard immunoassay procedures. The results demonstrated a purity of greater than 98% and a composition of human IgG. The purified human anti-HIV was divided into aliquots and frozen for subsequent use in experiments and procedures disclosed below. Those skilled in the art will be familiar with methods for characterizing both antigen and antibody pools to define specificities which may be used in subsequent determinations against unknown antibodies or antigens for comparative purposes.

As described in the following examples, this human anti-HIV was employed to follow the purification of HIV proteins from crude viral lysates and map HIV epitopes recognized by the human immune system and in competitive EIA to identify HIV epitopes targeted by antibodies produced in goats but not man. Western Blot analysis was employed to characterize antibody responses in goats immunized with either purified

HIV

proteins containing peptides of interest or synthetic versions of those peptides, and to evaluate the efficacy of a microparticle carrier complex designed to amplify immune responses to poorly immunogenic peptides. Antibodies which neutralized

HIV

infectivity were evaluated by a microculture procedure which employed purified human CD4 lymphocytes isolated from peripheral blood mononuclear cells (PBMC), employing standard techniques which use monoclonal antibody-conjugated magnetic particles to remove unwanted cells. CD4 lymphocytes were stimulated with mitogen to increase their susceptibility to HIV infection and were used throughout unless otherwise noted as the host target for HIV infection. HIV1s 2 was employed throughout unless otherwise noted as the reference HIV strain. The effect of antibody on HIV infectivity was determined by microculure using techniques familiar to those skilled in the art.

Infectivity is expressed as infectious units

(IU).

Antibody mediated reductions in IU were associated with neutralization of virus infectivity and expressed as change in infectious unit. The human anti-HIV pool was used throughout all experiments described herein that required human anti-HIV antibody. This human IgG anti- HIV pool was compared to several commercia-lly available human anti-HIV preparations and had equal or greater HIV neutralizing activity and, when compared by Western Blot analysis, was significantly more reactive.

Examcle 2 Characterization of Commeriavlly Available HIV Viral vsates Imoloved Hrein Preliminaryv Studies

HIV

1 MN, HIV1B and HIV2Nz were purchased from Advanced Bio-Technology, Inc., Columbia, MD, in the form of purified viral lysates. Analysis of these purified viral lysates demonstrated lot to lot variation in total protein content with a range of 0.8 mg/ml to 1.2 mg/ml. The protein composition of each HIV lysate was evaluated by SDS-PAGE and Western Blot analysis with human IgG anti-HIV. The HIV lysates were treated with protease inhibitors and nonionic detergents such as Nonidet P-40 or Igepal CA630, to fully dissociate HIV proteins and glycoproteins into their monomeric forms, and clarified by filtration through a 0.22 micron filter. Lipids were removed with SeroClear employing standard procedures. It is well known to those skilled in the art that HIV incorporates human proteins into its envelope as part of the budding process. Such contaminants once identified were removed by immunoaffinity chromatography. In the initial purification step serum contaminants present in growth media added to facilitate cell growth and contaminants in the HIV lysate were removed by immunoaffinity chromatography employing anti-normal human serum Sepharose CL6B 2 3 mg antibody/gram Sepharose).

Chromatography of HIV lysates were conducted at a matrix to lysate ratio of 1:1 volume/volume at a flow rate of 10 ml/hour. The chromatography and elution were monitored spectrophotometrically at a wave length of 280nm. The protein rich non-binding fraction containing the HIV related proteins was concentrated to 1 mg protein/ml and stored at -70°C for future use as needed. Proteins bound by the affinity matrices were eluted with glycine-HC! buffer pH 2.2 in 0.9% .NaCl.

Eluates were neutralized, dialyzed in PBS pH 7.8 containing 0.1% Igepal CA630, concentrated and stored at -70°C for future analysis. SDS-PAGE with Western Blot analysis of purified HIV preparations consistently demonstrated the presence of gpl60, gp!20, p66/55, gp4l, pl0, p24, p17 and p7, employing human anti-HIV IgG. HIV proteins were not detected in glycine HC1 eluate employing Western Blot analysis, but SDS-PAGE gels stained with coomassie brilliant blue for protein visualization demonstrated 2-3 weakly stained bands.

aracterzation of Anti-HIV Antibody Produced to Partially Purified HIV Lysates Goats were immunized with the purified

HIV

proteins obtained above and responded immunoldgically with antibodies which reacted with immunodominant epitopes on HIV. Further evaluation of this antiserum demonstrated the presence of cytotoxic antibodies which reacted with both HIV-infected and non-infected CD4+ lymphocytes, and red blood cell (RBC) agglutinins were detected. These agglutinins were reacted with all human RBC blood groups and RBC's from rabbits and guinea pigs. Two possibilitie s for these unwanted antibody specificities were considered. One possibility was contamination from HIV lysates with proteins of cell culture origin, and the second was mimicry between HIV proteins/glycoproteins and glycoproteins found in man and other animal species It is well known that host membrane proteins are often identified in the envelope of HIV. The incorporation of host membrane components into the envelope of HIV is thought to be non-specific and associated with the budding of the mature virion. Two such proteins previously identified in mature virion envelope are human HLA class I and class II antigens. Both

HLA

class I and class II antigens were quantitated employing an enzyme linked immunoassay and results demonstrated the presence of both HLA class I and class II antigens in these HIV preparations and at concentrations disproportionate to their concentration measured in cell membrane preparations derived from uninfected culture cells. Further studies confirmed the presence of HLA class I and class I! antigens in different preparations (n=17) of HIV viral lysate. The measured concentration in these preparations was variable but consistently 10 to 100 times greater than that measured in membrane extracts from uninfected control cells. The goat anti-HIV antibody was tested by Western Blot Analysis against known HLA class I and.

class II isolates and confirmed to contain antibody specificities directed against HLA class I, HLA class II (alpha and beta chain) and beta 2 microglobulin.

This antibody was evaluated for'HLA allotype specificity. Commercial trays containing lymphocytes of known allotypes were employed as targets. Under assay conditions, this antibody was cytotoxic to all lymphocytes, and this cytotoxicity was partially inhibited with soluble HLA class I and HLA class II in a dose dependent manner. (Table 2.1).

TABLE 2.1 HLA Class I Inhibition of Lymphocytotoxicity in Antiserum Produced to Purified HIV Lysates Anti-HIV Ani-HIV Anti-HIV Anti-HIV Dilution& HLAI& HLAIII HLAI&I HLAI&IT IT lution Ati- V 25 ug 50 ug 100 ug 200 ug Sf 8 8 8 8 1:10 8 4 4 4 1:20 8 8 8 0 1:0 8 2 4 4 1:40 8 8 0 0 0 1:80 4 1:160 8 1 320 8 2 0 0 0 1:640 a 0 0 0 0 1:1280 40 0 0 0 Soluble HLA class I&iI was added to micro titer wells containing anti HIV antibody and incubated overnight at 4hadC. Soluble HLA I&II reduced lymphocytotoxicity but had no additional effect at concentrations above Further studies demonstrated an antibody that reacted with a phylogenetically preserved carbohydrate antigen present on gpl20, human red and white blood cells, and red blood cells from different animals including rabbit, rat, and guinea pig. Absorption studies with human and rabbit red cells completely 2 -5 removed the remaining antibody activity to both RBC's (Table 2.2) and lymphocytes (Table 2.3) following absorption with S-HLA-I

II.

62 TABLE 2.2 Analysis of Anti REC Absorbed Anti-HIV Antibody for REC Agglutinins Cell's *Antisera produced to Purified REV lysate resulted in RBC agglutinins produced in gas following immunization. Goats (n=2 each) were immunized with HTV1,M and HIVSA:L andor HIV2Nz, respectively.

TABLE 2.3 Analysis of Anti REC Absorbed Anti-HIV Antibody for Lyrnphocytotoxicity During Immunization *Antisera Produced to HIV without glycosidase treatment week blood collection date and absorbed 0-5 times thred blood cells and tested against lymphocytes for Following the absorbtion and removal of antibody specifities to HLA and hemoagglutinins the goat anti- HIV antibody was identified to contain specifities similar to those previously desribed in the literature This information demonstrated the need for modification of the HIV proteins to remove carbohydrate and HLA antigens to avoid the generation of cytotoxic antibodies directed against human antigens.

Example 3 Purification and HLA Class I and Class II Antigen Removal and Carbohydrate Removal of HIV Protein Isolated from HIV Lysates HIV lysates of HIV1,,, HIV1I and HIV2Nz were purchased from Advanced Biotechnologies, Columbia,

MD.

They contained protein concentrations in the range 0.8 1.2 mg protein/ml. Protease inhibitors were added to protect the proteins from degradation, and non-ionic detergent (Igepal Ca-630, or Nonidet P-40) was added to dissociate HIV proteins. The mixture was dispensed into capped extraction tubes with a delipidating reagent to remove lipids and clarify the mixture.

Lipids were differentially dissolved in the organic layer after centrifugation and the aqueous phase was removed.

Contaminants of cell culture origin, including HLA, were removed by immunoaffinity chromatography on five separate affinity matrices prepared by the covalent attachment of: i munoaffinity purified polyclonal IgG antibodies to human serum proteins, monoclonal IgG antibody to HLA-1, monoclonal IgG antibody to HLA-2, monoclonal antibody to (2microglobulin, immunoaffinity purified polyclonal IgG antibodies to lymphocyte and red blood cell membrane antigens. Each matrix contained 2-3 mg antibody per g Sepharose CL6B. C -umns were configured in a tandem arrangement, and matrices were poured and equilibrated with PBS at pH 7.8 containing 0.1% Igepal Ca-630.

Lysate solutions in volume equivalent to column bed volume were successively chromatographed throuah each column at a flowrate of 10 ml/h. The chromatography and elution were monitored spectrophotometricallv at a wavelength of 280 nm. The protein-rich non-binding fraction containing the HIV related proteins were concentrated to 1 mg protein/ml.

SDS was added to the immunoaffinity-purified mixture containing the HIV proteins of interest and the mixture was heated at 70 'C for 10 minutes. The protein were enzymatically deglycosylated using PGNase.

The protein mixture was fractionated by size chromatography on Sepharose G50 pre-equilibrated with saline containing 0.1% non-ionic detergent. Protein fractions were collected and those containing the desired HIV proteins were individually pooled. The three protein pools, individually enriched for and gp120, p 6 6/55 and gp4l, and p24, p17 and p10 were retained. Fractions were stored at -70 °C until further processing.

Detailed methods for quantitation and removal of human leukocyte antigen (HLA) are disclosed in "Identification, Characterisation, and Quantitation of Soluble HLA Antigens in Circulation and Peritoneal Dialysate of Renal Patients", F. Gelder, Annals of Surgery Vol. 213, (1991), incorporated herein by reference.

Table 3.1 shows data obtained from the purification.

TABLE 3.1 Purification Of HIV Lysate Fractionation Protein Volume Total HLU I HLA !I Step mg/ml ml Protein rig/ml ng Recovery H~IV Lysate 1.13 10 11.3 183 62 Post Lipid Removal 0.96 10.3 9.8. 1726 683 86.7 Post Affinity Chromatography 0.44 19.5 8.58 (10 (10 75.9 Post Concentrat'o 1.0 8. 1 Q.1 10 <0 7.

IuV preparations purified as described typically were devoid of contaminants, including HLA class I or class SDS-PAGE with Western Blot analysis Off purified HIV preparations consistently demonstrated the presence of gp16O, gpl2O, p66/55, gp4l, p10, p24, p17 and p7 employing the pooled human IgG anti

HIV.

Exarnole 4 Pearation and Characterization of the Biochemical and Conformational Requirements of MDP Microparticle for Eliciting Adjuvant Effects with Purified

HIV

A multiple repeat of muramyl dipeptide.(MDP) isoLated from Promoionibacterium acini, formed the core structure of the MIDP microcarrier complex of this example. The chem'cal composition of the monomeric subunit is

MDP

0

-OH

HO

NHAc CH3CHO-NHCH

(CH

3 CO-NHCH (CH2CH2-COOH)

CONH

2 MDP has well known immunostimulatory properties which have been extensively evaluated in studies designed to determine its effect on increasing immune function. Those skilled in the art are familiar with this effect.

To date, both MDP isolated from natural sources and synthetic MDP have been associated with signficant toxicity when administered to mammals. This toxicity has limited the effectivenes of MDP as a carrier.

A method for the isolation of MDP free from toxic components is provided herein. Propionibacterium acini was grown to a mid-stationary growth phase and washed to remove contaminants of bacterial culture origin employing techniques well know to those in the art.

Hydrophobic components contained in the cell walls and cytoplasm were sequentially extracted by successive washes in gradual concentrations of ethanol/methanol/water at elevated temperatures. The resulting MDP microparticle was suspended in ethanol and its concentration was measured by relatin s sorbanc at 50elating its absorbance at 540 nm to the absorbance of turbidity standards. The concentration of the MDP microparticle was adjusted to 1 mg/m l for Storage and later use.

Analysis of this preparation demonstrated muramy! dsipetide extensively crosslinked with a microparticle size of to 0.2 micron. The terminal dipeptide amino-linked L-alanine-D-isoglutamine was identical to the monomeric structure shown above. It is well known that there can be differences between bacterial strains and these differences can result in differences in peptide composition, such as terminal peptides with five or more amino acids, changes in dipeptide amino acid composition, in particular L-alanine-Lisoglutamine, and sites where O-acylated beta myristate groups have been incorporated. These are not desirable and account for toxicity and poor adjuvant properties of MDP isolated from natural sources. In a preferred embodiment the MDP microparticles (0.01-0.2 micron; preferrably 0.05-0.1) have amino-linked L-alanine-Disoglutamine dipeptide. Such a microparticle can be isolated from natural sources, as above, or synthesized using well known synthetic procedures.

Examole Preparation and Preliminary Evaluation of MDP-Immunogen Conjugate The adjuvant effect of the MDP microparticle (0.2u) of the preceding example on antibody production was evaluated employing a poorly immunogenic monoclonal human lambda light chain fragment lacking approximately 22 amino acids at the sulfhydryl bridge (Mr 18,000) as the immunogen Two conjugates were made, one in which the immunogen was covalently conjugated to MDP through the carboxyl terminal group and one wherein conjugation was through the amino terminal group.

M DP-immunogen conjugates were assembled in a stepwise manner and reaent exchange was performed after each step by centrifugation, supernatant removal and replacement with the required reagent to continue the conjugation sequentially through MDP:immunogen assembly. The molar ratios that reaction and the reashown w i t h e a c h reaction andthe reagent exchange that was performed after each Step prevented multiple point attachment of the immnogeificantly which, from reliminary experiments, significantly reduced immune antibody responses.

Synthesis of MDP:NH2:i mmunoaen.o 2 Protocol for Efficient Two-Step Coupling of

HIV

Proteins to Muramyl Dipeptide Using

EDC

1peptide Using

EDC

The following procedure adaptedfrom a procedure described by Grabarek z. and Gergely, Arocedure Biochem. 185:1311 (1990) allows for seqent J. Aal coupling of HIV proteins and peptides to MD w sequen ithout exposing the HIV protein to -ethyl (3 i d e s t o MDP ithout adme(hylaminoropyl)carbodiimide (EDC) and thus qu affecting carboxyls on HIV. This procedure calls for quenching the first reaction with a thiol compound.

The reaction is carried out in 2 [N-morpholinoethane sulfonic acid (MES) (pH 4.5-5.0).

MDP (10 mg) lyophilized from water resuspended in 2 0 MES (0.5 ml) (pH 4.5-5 e f r o m w a t e r resuspended in issolve in MES (pH 45-5.0) and 0 5 ml .EDC 0 .5mg~2mM) dissolved in MES (pH 4.5-5.0) were combined and reacted for 15 minutes at room tem w eratur e c mb i n e d a n d to ate d 'final concentration of em p e r a t u r e 2 I mercaptoethanol fEDnal concentration of 20 mM) was added to quench the D nd serted by centrifugation. The reaction mixture was washed Once with MES and resuspended in ml ME (pH 4.5-MES was The human e light chain fragment issolved in MES was added to the activated MDP at a mslowlar ratio of about 2:1. The PH of the reaction was sl 30 ddowly reused over a 15 minute period to 8.5 by the 0 addition of teMES (raturM pH 8.5) and reacted for 2 hours at room temperature. The concentration of the light chain fragment added to MDP was calculated from quantitative analysis of MDP terminal

CO

2 H group and expressed as mole COH per mg D i n a l The CO H n Was S gD-* Te reaction was quenched by adding hydroxylamine to a final concentration of 10 mM. This method of quenching hydrolyzed any unreacted MDP activation sites and resulted in regeneration of the original carboxyls.

Other means of quenching involve adding 20-50 mM Tris, lysine, glycine, or ethanolamine; however, these primary amine-containing compounds will result in modified carboxyls MDP. When the MDP is used with an HIV synthetic peptide and modification of that peptide is desired, such as to change hydrophobicity or to attach bio-active compounds, the modification can be attained by adding the desired compound prior to the final quench step. This permits the desired compounds to be added sequentially following the initial coupling with HIV peptides. Bioactive peptides also could be added at desired ratios with HIV peptides as a single step when greater control of peptide immunogenerity is desired. Biological response modifiers such as IL2 are well known to those skilled in the art and could be used for this purpose.

Separation was achieved by centrifugation, a wash step, and resuspension in the buffer of choice.

PREPARATION OF MDP-CO2H-IMMUNOGEN-NH, Svnthesis of MDP..rHnoHaH:C Protocol for Efficient Three-Step Coupling of Proteins to Muramyv! Dipeptide Using

EDC

This procedure allows for sequential coupling of a protein or peptides to MDP without exposing the protein to EDC and thus affecting amino groups on the protein.

The procedure employs two intermediate steps conducted sequentiallyS The initial reaction is carried out in MES (pH 4.5-5C.0). MDP (10 mg) lyophilized from water resuspended in 0.5 ml MES pH4.5 and 0.5m1

EDC

(0.5mg-2mM) dissolved in MES were combined and reacted for 15 minutes at room temperature. Excess EDC was quenched by the addition of 2 -mercaptoethano! (final concentration of 20 mM), and the activated.MDP was separated by centrifugation, washed two times with

MES

and resuspended in 0.5 ml MES (pH Diaminoethane (NH2CH2CH2NH2), dissolved in MES( pH4.5) was added to the activated MD? at a molar ratio of about 10:1. The pH was slowly increased over a 15 minute period by the addition of MES 0.5M pHS.5 and reacted for 1 hour at room temperature. MDP:NH2CH2CH2NH2 was separated by centrifugation, washed two times with MES and resuspended in 0.5 ml MES pH 4.5. The X light chain fragment was suspended in 0.5 ml MES pH4.5, and EDC (0.5mg 2mM) dissolved in MES was added and reacted for 15 minutes at room temperature. Excess EDC was quenched by the addition of 2 -mercaptoethanol (final concentration of 200 and the activated protein was separated from excess reducing agents and inactivated crosslinkers by size chromatography on an appropriate size gel filtration column. The activated protein was added to the activated MDP:NH2CH 2

CH

2 NH2 at a molar ratio of about 5:1 and reacted for 2 hours at room hours at room temperature. The concentration of protein added to MDP was calculated from quantitative analysis of MD and resuted in regenerat of the rigianalsis of MDP ermIf an HIV synthetic peptide is used as mothe immunogenr mg to a final concentration i hyro.a and modification if that peptide is desired, such as to change hydrophobicity of that peptide or attach bioactive compounds, the modification can be accomplished as described above. Separation was achieved by centrifugation, a wash step, and resuspension in the buffer of choice.

It should be noted that bioactive compounds may require the intermediate step described above when attachment through the CO 2 H group is desired.

Example 6 Comparative Study of MDP Immunogen Conjugate Against Commercial Adjuvants Including Freunds Complete Adjuvant, RIBI®, Titer Max® and Alum The adjuvant effect of this MDP microparticle (0.1u) on antibody production was evaluated employing the poorly immunogenic monoclonal human lambda light chain fragment described in the preceding example (Mr 18,000) as the immunogen Two conjugates were prepared, one in which the immunogen was covalently conjugated to MDP through the carboxyl terminal group and one wherein the conjugation was through the amino terminal group.

Rabbits (n=5 each group) were immunized subcutaneously with approximately 100 micrograms of lambda light chain attached to 500 microgram MDP and emulsified in squalene. Animals were immunized at monthly intervals and test bleeds were obtained prior to immunization and at two week intervals throughout.

The antibody responses to MDP:NH2-I-CO2H and MDP:NH2CH2CH 2

NH

2 :HO2C-I-NH2 were comparable in activity; however, the MDP:NH2-I-CO2H-stimulated rabbits produced at least one additional antibody specificity determined by competetive EIA. The antibody responses obtained were compared to those obtained when conventional adjuvants were employed for antibody response, including Freund's complete adjuvant, Ribi®, Titer Max® and Alum (aluminum hydroxide). Both MDP:HO2C-I-NH 2 and MDP: NHi2-I-00) 2 H were Significantly superior to the conventional adjuvants with immunogen in inducing antibody. Immrunogen concentration (100 ug/iromunization) and immrunization schedule were identical in all groups. Table 6.1 shows the antibody.

titer measured at bi-weekly intervals and titer is expressed as the reciprocal of the dilution producing a positive reaction as described above. Both

MD?

conjugates were superior to conventional and well known adjuvants.

TABLE 6.1 Week *IMP +HDP:I Fr~eund' RIbi Titer Alu (T-0 154 6 164 8 256 128- 512 512 44 1 2 1024 512 16 16 36 14 4096 2048 32 32 64 4 16 4096 4096 64 128 256 8 18 8192 4096 256 512 1024 32 16384 8192 256 512 2048 32 T-0 Primary immuniz7ation and pre-imm'unization blood collection.

MDP:I muramyl d-ipeptLide.muce ir atce 0.2m).~.mmngn mir atce Peptide was conljugated at amino terminal group to isoglutamine with carboxy terminus exposed. (MDP:I:Co 2

H)

Peptide was conjugated at carboxy terminus through an intermediate step employing diamninoethylene to modify the carboxyl terminus of MDP.

(MDP:I:NH

2 Example 7 Cytokine Response of Peripheral Blood Mononuclear Cells Induced with MDP:NH 2

:I:CO

2 Hand MDP:NH2CH2CH2NH 2 :O2HC-I- NH2Immunogens To further evaluate the mechanisms associated with the increased antibody response to the MDP microparticle-immunogen complexes, an in vitro method which measured cytokine production or peripheral blood mononuclear cells was employed and compared to known cytokine inducers. Lipopolysaccharide (LPS) and LPS with phytohemagglutinin (PHA) were employed as known cytokine inducers. Cytokines were quantitated employing a well-established assay and expressed as units/ml. Peripheral blood mononuclear cells were isolated by Ficol Hypague gradient centrifugation and adjusted to a concentration of 2 x 1 0 6 /ml in tissue culture media. Cells (100ul) were plated into microculture wells.

MDP:I:CO

2 H, MDP:I:NH2 PHA

LPS,

LPS and media alone were added undiluted or diluted 1:10 and 1:25 (10 ul). Cultures were incubated at 370°C in a 5% CO2 atmosphere for 48 hours and supernatants were removed and assayed by standard bioassays and/or EIA methods.

TABLE 7.1 IFNR IL2 J TNF IL6 MDP: I:COH 550 510 152 62

MDP:I:NH

2 520 495 176 73 LPS PHA 340 320 495 450 LPS 210 150 325 310 MEDIA 0 0- 0 I :CCH 496 398 68 2 NH2 23 410 72 21 LPS PHA 205 165 210 152 1 0 LPS 175 90 135 140 I:NH2 51 e85 9 LPS PHA 20 10 15 LPS 20 10 15 Both MDP:I:COH and MD-P:I:NH2 stimulated greater Type I cytokine responses ethan LPS +PHA or LPS alone. Type 1 cytokine responses enhanced immune events while Type 2 cytokine response is indicated by elevated IFN gamma and IL2 and lower levels of TNF and IL6. I FN ExamnIle 8 Characterization of the Antibody Response in Goats to HIV Proteins Untreated and Treated to Remove Carbohydrates Moieties and Comparison of the Adjuvant Properties of MDP Micropartices with conventional Adjuvants croparticles with Conventional Example 8.1 HIVl~N HIVIBA, and HIV2NZ viral lysates were purchased from Advanced Biotechnologies Inc., Columbia, MD. and one-half of each preparation was treated enzymatically to remove carbohydrate and all preparations (with and without carbohydate) were purified as above. An aliquot of each was conjugated to MDP through the amino terminal residue in accordance with the procedures set forth in Example 5, and individually suspended in squalene. For comparison, these HIV proteins also were emulsified in Freund's complete adjuvant without conjugation to MDP.

Group 1 HIVI HIV1, 1:1, without carbohydrate removal; Group 2 HIVI~ HIV1, 1:1, with carbohydrate removal; Group 3 HIV2m z without carbohydrate removal; Group 4 HIV 2 Nz, with carbohydrate removal Group 5 HIV1 HIVlu HIV2mz 1:1:1, without carbohydrate removal; Group 6. HIVl~ HIV1L HIV2,z 1:1:1, with carbohydrate removal; Group 7 HIV1, HIVlz, HIV2Nz, 1:1:1, without carbohydrate removal and emulsified in Freund's complete adjuvant without conjugation to MDP.

Group 8 HIV1N: HIVlBA:

HIV

2 Nz, 1:1:1, with carbohydrate removal and emulsified in Freund's complete adjuvant with conjugation to MDP.

Goats were stratified into immunization groups 1-8 (n=3 each) and respectively immunized at the intervals shown in Table 8.1 with 100 ug HIV/immunization. Blood.

samples were obtained prior to immunization and at biweekly intervals. Antibody reactivity was quantitated by EIA using the FDA approved commercially available test kit from Abbott Laboratories. Results were expressed as the reciprocal of the antisera dilution that produce an absorbance value TABLE 8.1 Analysis of Anti HIV Antibody Response to HIV Proteins T-0 Primary immunization and pre-immunization blood collection I Carbohydrates removed Immunization Booster The antibody responses were measured at bi-weekly intervals throughout immunization. There was no significant difference in antibody reactivity, measured by EIA, between individual animal groups (Groups 1&2, 3&4, 5&6) with MDP. Carbohydrate removal had no effect on production of antibody to the desired anti-HIV proteins.

Example 8.2 Antisera obtained as described in Example 8.1 were evlauated for hemagglutinating antibodies. As can be seen from Table 8.2, carbohydrate removal from the HIV proteins obliterated the hemagglutination response.

TABLE 8.2 Analysis of Anti HIV Antibody Response to Red Blood Cells Group 2 Group 3 Group 4 Group 5 Group 6 Group 7 Group 8 \ntj HiJVl' Anti HIV2 Anti HIV2 2 Anti IHV1&2 Anti HIV1&21 Anti -II&2 Anti. HIVI&2' 2 4 4 4 -4 T-0 Primary immuniz~ation and pre-inununization. blood collection 1-Carbohydrates removed -Immunization Booster HIV preparations treated to remove carbohydrate groups failed to produce antibody reactivities able to agglutinate red blood cells (Table 8.2A). Goats immunized with MDP-HIV conjugates without carbohydrate depletion and a goat .immunized with purified

HIV

without carbohydrate depletion emulsified in Freund's complete adjuvant produced red cell agglutinins. There was no detectable difference in the titer of red cell agglutinins in antisera from goats immunized with MDP- HIV conjugates or HIV emulsified in Freund's complete adjuvant. The red cell agglutinins described herein were essentially identical to those described in the preliminary studies of Example 2. These agglutinins were cytotoxic (Table 8.2A). However, there was no detectable antibody reactivity to HLA class I or class II and absorption with red blood cells completely removed both the hemagglutinating and the cytotoxic antibody reactivity.

TABLE 8.2A Sequential Analysis of Anti HIV Antibodies for Lymphocytotoxicity Group 2 Group 3 Group 4 Group 5 Group 6 Group 7 Group B Anti HIVI' Anti HIV2 Anti HIV2 2 Anti HIV1&2 Anti HIV1&2' Anti HIVI&2 Anti HIVI&21 1- 2± +2 +4 4 f2 0 T-0O Primary immunization and pre-immunization blood collection 1-Carbohydrates removed *-Immunization Booster To evaluate the possibility of mimicry between

HIV

and RBC carbohydrate groups, additional antisera were produced by immunizing a goat with an immunogen composed of purified and pooled cell membranes isolated from human red blood cells. Western Blot analysis employing this antisera demonstrated reactivity with a red blood cell glycoprotein (-Mr35,000) and reacted with HIV gp4i and gpl20. However, HIV gp4l and treated to remove carbohydrate groups were unreactive.

These data were consistent with phylogenetic mimicry between carbohydrate epitope on HIV and red blood cell glycoproteins.

Western Blot analysis demonstrated strong reactivity to most HIV proteins, including gpl20, gp4l, p66/55, plO, p24, p17 and p7. There was no apparent difference in the reactivity or specificity of these antibodies to the HIV epitopes disclosed herein. These antibodies reacted to all HIV isolates tested, including those which have been shown to have resistance to reverse transcriptase and protease inhibitors.

Example 8.3 Neutralization of HIV Infectivity by Antibodies produced to Carbohydrate-Depleted

HIV

2S This example describes and characterizes the neutralization of HIV infectivity using the antibodies produced to carbohydrate depleted HIV disclosed above.

The results indicate that these antibodies contain high levels of neutralizing activity and protect CEM cells from-infection in a dose dependent manner.

Neutralization Assay: A sensitive neutralization assay was employed to quantitate the effect of goat anti HIV on HIV infectivity. The CE M CD4' cell line, which is highly susceptible to HIV infection, was chosen as the target cell to determine the effect of this -ant~ibody on HIV infectivity. The antibody and dilutions were made as required in RPMI medium containing 10% fetal calf serum.. A suspension of HIVlSF 2 was harvested from about four-day cultures of OEM in log growth phase, filtered through 0.2 or 0.45 micron filters, aJliquoted, and frozen at -700 C. One aliquot was thawed, titrated to determin~e the TCID5o, and subsequent assays were performed with freshly thawed al~iquots, diluted 1:500 in culture medium to a concentration of approximately ten times the amount required to infect 50% of OEM cells in culture (10 TCIDso) The virus suspension was mixed with an equal volum'e (250 ul) of five-fold dilutions of antibody from 1:S to 1:9,765,625. The virus/antibody mixture was incubated for 60 minutes at 370 C. and duplicate samples of 200 ul used to inoculate wells containing 1.0 ml of approximately 2x10 CEM cells per well. The cultures were incubated at 370 C. in a humidified, 5% C02 atmosphere for 14 days. The cells were harvested, pelleted, and lysed with 1% Triton X-100 in PBS for about 10 minutes. The amount of virus (or viral antigen) present in lysed cells was quantitated using a commercially available p24 assay.

The titer of-L7 neutralizing activity was determined as tne reciprocal of the dilution of" anti body which inhibited p24 antigen production by greater than 50% of virus control Cultures incubated without antibody, or With goat pre-immune IgG prepared in a similar maniner.

Two hundred microliters of the lysed cellular suspension were assayed.

TABLE 8.3A Analysis of Anti [HIV Antibody Neutralization Activity W~ee k Group I Group 2 Group 3 Group 4 Group 5 Group 6 Group 7 Group 8 It Anti fl1I Anti IJIVI' Anti 111V2 Anti 1V2' Anti HIV1&2 Anti HIVl&2' Anti 111V1&2 Anti IIVI&2

T-~OA

2 21 2024 516/1098 2048/5 2526 406/1 10240 2/2 T-0 Primary immunization and pre-immunization blood collection I Carbohydrates removed =anti-HlIv prepar-ations obtained from the week 20 bleeds were absorbed with human red blood cells and both unabsorbed and absorbed samples were tested under identical conditions. Results are expressed as (unabsorbed/absorbed) neutralization titer.

The anti HIV preparations with the greatest neutralizing activity were produced to HIV-MDP conjugates that were not treated to remove carbohydrate determinants (Table 8.3A). Red blood cell absorption of antibodies to carbohydrate-depleted HIV conjugates had no effect. However, RBC absorption of antibodies to carbohydrate-intact HIV conjugates resulted in a significant reduction in neutralizing reactivity, confirming the presence of phylogenetically present carbohydrate moieties shared between HIV and humans (bottom row, Table 8.3A). All anti-HIV antibody preparations produced to HIV-MDP conjugates were statistically greater than anti-HIV produced using Freund's complete adjuvant. Due to the predicted genetic variability characteristic of HIV, anti-HIV from the 20 week preparations were tested for neutralizing activity using HIVmN and four HIV wild isolates, including one characterized as a multi-drug resistant strain under conditions identical to those described above. Anti-HIV from the 20 week antibody preparations produced to HIV conjugates devoid of carbohydrate neutralized all strains (Table 8.3B).

Those skilled in the art will recognize that the neutralizing activity produced to HLA-depleted, carbohydrate-depleted HIV proteins is considerably higher than the neutralizing activity typically observed in human anti-HIV sera.

TABLE 8.3B Analysis of Anti HIV Antibody Neutralization Activity Against Multiple Strains HIV Strain Ant Anti _Anti MS21 Pre =mune Isolate H j ti IV12 1 IgG HIVSF 10192 512 20384 0 HIVMN 5096 256 10192 0 Wild#1 10192 512 20384 0 Wild#2 2048 256 5096 0 ild3 204 128 5096 0 1=carbohydrates removed Example 8.4 Effect of Anti HIV on HIV Infected CD4 Lymphocytes in an Antibody Dependent Complement Mediated Cytotoxicity Antibodies against carbohydrate-depleted and carbohydrate-intact HIV conjugates were evaluated for complement mediated cytotoxicity reactivity to normal peripheral blood mononuclear cells enriched for CD4 lymphocytes with and without infection with

HIV.

Normal peripheral mononuclear cells were isolated, subjected to Ficol Hypaque gradient centrifugation and enriched for CD4- lymphocytes. CD4* lymphocytes were stimulated with PHA and infected with HIVMN for 7 days in micro culture. Supernatants were removed and replaced with anti HIV produced to HIV preparations following sugar group removal and shown to have no cytotoxic effects on normal cells. As can be seen in Table 8.4, anti HIV lysed infected CD4 lymphocytes in a dose dependent fashion.

TABLE 8.4 Analysis of Anti [HIV Antibody Mediated Cytotoxicity of Infected CD4 Lymphocytes Group 1 Group 2 Group 3 Group 4 Group 5 Group 6 Group 7 Group 8 Anti HJIVi Anti JiIIv' Anti 111V2 Anti HIV2' Anti HIV1&2 Anti HIV1&2' Anti HIVI&2 Anti HII.2' 4 4 2 4 4 8 162 32 32 .32 32 44 256 256 2- 128 64 T-0 Primary immunization and pre-immunization blood collection I Carbohydrates removed *-Immunization Booster Example Synthesis of Peptides Corresponding to the Amino Acid Sequence of HIV Proteins Synthetic peptides were constructed as twelve-mer peptides which mimic the amino acid sequence of HIV1 Amino acid sequences for gp 120, gp 41, Vif, gag p 17, gag p 24, nef, Rev, Integrase, Protease, Tat:HxB2 and Reverse Transcriptase and Reverse Transcriptase with overlaps by six amino acid residues were synthesized by and purchased from Purification Systems, Inc.

employing solid phase technology.

Butyloxycarbonyl-S-4-methylbenzyl-L-cystine coupled to polystyrene using dicyclohexylcarbodiimide with a catalytic amount of 4-N,N-dimethylaminopyridine was used as the solid-phase support for the synthesis The amino groups were protected with tert-butyloxycarbonyl (t-BOC) and the side chain protecting groups were as follows: benzyl ether for the hydroxyl of serine, dichlorobenzyl ether for the phenolic hydroxyl of tyrosine, and the beta benzyl-esters were used for the carboxyl groups on glutamic acid and aspartic acid, respectively.

Trifluoroacetic acid (40% in CH 2 C1 2 was used to remove t-BOC and the resulting salt was neutralized with N-diisopropylethylamine (10% in CHC12).

Diisopropylcarbodiimide was used to couple the t-BOC amino acids. The protecting groups were removed and ne Peptide was cleaved from the resin at 0' degrees

C.

with anhydrous hydrogen fluoride containing anisole and 1% ethanedithiol as scavengers. The hydrogen fluoride reagent was removed under vacuum at 00 C and the peptide then was precipitated and washed with anhydrous ether. After extraction of the peptide from the resin with trifluoroacetic acid, the solvent was evaporated to 15° C. and the peptide was again precipitated with ether. The ether was decanted after centrifugation and the pellet was dissolved in 5% acetic acid with 6 M guanidine

HC

l This solution was desalted on a BioGel P2 column equilibrated in acetic acid and the peptide containing fractions were pooled and lyophilized. A cysteine residue was added to the carboxyl terminus of the peptide as needed to provide a functional SH group for the coupling of the l0 peptide to carrier proteins or to a solid support for EIA procedures or to MDP (Example When multiple repeats of the peptide were desired, synthesis was conducted by first attaching a cysteine residue to the resin support. Carbon spacers of various lengths were added; the choice of spacer length varied and was dependent on the application, peptide charge and length and steric influences predicted from preliminary data resulting from peptide attachments to supports. A six carbon spacer such as 6-aminohexanoic acid was first desattached with lysine- (ysine)2-(lysine)4 additions as described above with diaminoethane in Example 5 but altering the sequence of protective group blocking.

Amino groups were protected and then deprotected to permit two lysine residues to attach to the deprotected amino terminus, deprotection followed by lysine addition built a branched chain structure for peptide synor withesis Peptides with specific biological function or with sequences that are susceptible to enzymatic degradation were modified by the addition of D-amino acids. One particularly useful addition is the addition of L-alanine-D-isoglutamine with the peptide of interest synthesized off of the

NH

2 terminus of D- Isoglutamine. In another arrangement, the peptide was synthesized with L-Lysine-L-Lysine-peptide isoglutamine. The carboxy terminal lysine groups are highly susceptible to enzyme degradation by many enzymes in the micro environment while D-isoglutamine both results in an increase in half life of the peptide and provides a hydrophobic site to assemble peptides that require amphapathic properties to elicit a function such as receptor binding and immune induction through MHC associated events. A tyrosine residue was added to the amino terminus for radioactive labeling with 5 Iodine to determine peptide-to-carrier protein coupling efficiency and to identify the peptide during purification. 125I also provided a tracer to follow the half life of the peptide in biological systems and evaluate receptor binding when peptide function was not affected by tyrosine addition.

Examole 8.6 The use of synthetic peptides which mimic peptide sequences of HIV and other retroviruses, for the identification of viral epitopes Within this invention, synthetic peptide sequences which mimic highly conserved sequences found in HIV and other retroviruses are disclosed and their functional sigificance as immunological targets in treating viral infections are identified. These peptides have further application in the diagnosis and management of HIV infection resulting from HIV microvariants with sequences that contribute to the pathogenesis of HIV through nonspecific down regulation of immune reactions, induction of autoimmunity, and through toxic effects leading to HIV-associated peripheral neuropathy. Each of these events, when presented in a patient, contribute to the pathogenesis of HIV and decline in the quality of life. Synthetic peptides employed to identify and quantitate those HIV-peptide regions which initate those events, have utility in identifying risk factors for autoimmunity and peripheral neuropathy. The synthetic peptides provide further utility in a novel assay procedure to monitor disease progression and changes in progression as a result of treatment.

In one step of this invention, an enzyme immunoassay (EIA) was configured for the identification of goat anitbody specificities on HIV not recognized by human anti-HIV. Purified preparations of HIV1 and gp41 proteins were coated on wells of polyvinyl microtitre plates at 5 ug/ml in phosphate buffered saline (PBS< pH 7.8) by incubation for twenty hours at 37 0 C. The wells were washed with PBS containing 0.1% Tween 20 (PBS/Tween) and the unoccupied sites of each well were saturated with 5% bovine serum albumin by incubation for I hour at 37°C. The plates were used immediately or stored at 4° 0 C. Human IgG anti-HIV (100 ul) (Example 1) was added to each well, incubated for 25 hours at 4°C, and the wells were washed with PBS/Tween. Goat anti-HIV previously produced and labeled with HRP by standard procedures was added to the wells at a dilution required to yield an absorbance of 1.0 in the conditions of the assay (1:10000 titre) and incubated for 24 hours at 4C. The wells were washed and substrate added to determine amount of binding of the goat -gG. The percent inhibition of binding induced by blocking HIV proteins with human anti-HiV was-calculated using the formula.

(OD not bocked-D blocked x 1 00 OD blocked negative control OD not blocked Minimal blocking of goat anti-HIV-HRP conjugate by human anti-HIV was indicative of binding of goat anti- HIV different from human anti-HIV.

In another step, an EIA was configured to identify epitopes on HIV proteins that were antigenic with goat antibodies but not with human antibodies. Purified preparations of HIVI gpl20 and gp4l proteins were coated on polystyrene beads at 5 ug/ml in PBS by incubation for twenty hours at 370C. Beads were washed with PBS containing 0.1% Tween 20 (PBS/Tween) and the unoccupied sites on each bead were saturated with bovine serum albumin by incubation for 1 hour at 37 0 C. Beads were used immediately or stored at 40C. Human IgG anti-HIV (100 ul) (Example 1) was added to each bead, incubated for 24 hours at 40C. Human IgG anti- HIV (100 ul) (Example 1) was added to each bead, incubated for 24 hours at 4 0 C and the beads were washed with PBS/Tween. Synthetic peptides were dissolved in PBS containing bovine serum albumin (tmg/ml) and Tween at a concentration of .1 mg/ml. The peptides ul) were added to goat anti-HIV IgG-HRP conjugate solution (100 ul) and incubated for 24 hours at 4 0 C. The mixture then was added to two sets of beads coated with HiV. One set was blocked with human anti-HIV that was added to two sets of beads coated with HIV. One set was blocked with human anti-HIV that was added at the same time as the peptides were added to the goat anti-HIV-HRP conjugate. The beads with reactants were incubated for 24 hours at 40C. Following incubation, the beads were washed and peroxidase activity was measured as described above. Activity was plotted against peptide Position within the HIV proteins.

These plots showed areas of the HIV proteins targeted by goat HIV immune IgG that was not recognized by human antibody. When inhibition of binding was observed with a specific synthetic peptide, additional peptides were synthesized to overlap the original peptide by peptides of additional lengths. The lack of inhibition by the synthetic peptides was considered to represent lack of immunologic targeting by the goat immune system.

EmPloying this procedure, linear peptide epitopes were selected for study.

In another preferred procedure of this invention, an EA was configured to utilize peptide-peroxidase conjugates, produced as disclosed below, to identify antibody reactive epitopes on HIV proteins rcognized by the goat but not by the human immune system. Peptides mimicking HIV sequences were covalently attached to RRP to produce an enzyme labeled peptide library for use in mapping antibody specificity. Specifically, HRP was dispensed into a reaction tube at a calculated molar ratio of 1 part HRP:10 parts peptide. Each peptide to be coupled was individually dispensed into microtubes at a concentration of 0.1 1 umole/ml at volumes of 0.1 1 ml, each of which varied dependent upon quantity of conjugate desired and molecular weight to peptide. HRP at a concentration of 1- 1 0 umole/ml was dissolved in 0.2 M carbonate/bicarbonate buffer (pH 9.8) at 40C and sodium percodate was added to achieve a final concentration of 0.02 M. The mixture was immediately disspensed into the microtubes containing the peptides mixed and reacted for 30 minutes.

Ethylene glycol (0.02 N) was added to quench remaining sodium periodate The intermediate Schiff' s base formed between the amino terminus of the peptide and the aldehyde formed by oxidation of the

HRP

carbohydrate moieties was reduced by the addition of sodium borohydride (0.2 M) in water. Chromatography of each peptide-proxidase conjugate in Sdephadex G25 was used to remove reactants and excess peptide.

In this preferred assay, the antibody was used to bridge epitopes identified on the HIV proteins prepared 3 from HIV virus lysates and synthetic HIV peptides covalently attached to HRP since antibody reactive with HIV epitopes retained an antigen-binding site which could react with synthetic peptide epitopes atached to peroxidase. Beads were coated with HIV proteins as described above. Coated beads were reacted with goat anti-HIV IgG for 24 hours, the beads were washed and reacted with substrate to determine peroxidase activity and, therefore, peptide binding. With this procedure, only exact epitopes contained within the synthetic peptide were recognized. In this assay, the s omplete repertoire of goat rectivities was identified Human anti-HIV and goat anti-HIV reactivities were compared and peptides that reacted nly with goat anticomp were selected as candidate epitope targets. These data were benot considered to be all inclusive of differences between human and goat reacitvity to HIV proteins since differno ances between reactivities and shifts between the amroteino acid peptide sequence homologies on the

HIV

protein could result in missing epitope reactivity secondary to the selection of the synthetic peptide.

Example 9 Antibody Efficacy as a Therapeutic Agent A study was conducted with a group of imrmunochemically designed antibodies to determine the efficacy of the antibodies as a therapeutic agent for the treatment of HIV/ATDS. Specifically, a mixture of lysates of HIV isolates HIVIM- HIVy and IV 2 Nre ofas treated as in Example 3 to remove low molecular weight contaminants and HLA class I and class II antigens and to deglycosylate the H-IV proteins The protein mixture was assayed and found to comprise peptides which mixturic the following regions of HIVIs

E

2 proteins: r i s2 proteins: gp120: an epitope region extending from amino acid residue 4-27 and a second epitope region extending from amino acid residue 54-76; gp4l: an epitope region extending from amino acid residue 502-531; reverse transcriptase heterodimer p66/55: an epitope region extending from amino acid residue 254- 295; protease plo: an epitope region extending from 0 amino acid region 69-94; Gag gene protein p24: an epitope region extending from amino acid region 166-181; Gag gene protein pl7: an epitope region extending from amino acid region 2-23 and a second epitope region extending from amino acid region 89-122; and Gage gene protein p 7 an epitope region extending from amino acid region 390-41 and 438-443.

These amino acid sequences fail to elicit an immune response in humans when contacted through infection or naturally through the environment but do elicit an immune response in other mammalian species.

The purified and treated proteins were enriched and further purified using preparative

SDS-PAGE

electrophoresis and when desired, by immunoaffinity chromatography employing commercially available

(ICN

Costa Mesa, CA and Advanced Biotechnologies, Columbia, MD) monoclonal antibodies to gpl20, gp4l, pp 66/55, p24, p17 and p10 each individually coupled to Sepharose CL4B using procedures known in the art. Following purification, each purified HIV protein peptide of interest was individually conjugated to the MDP microparticle as described in Example 4.

HIV-MDP

microparticle conjugates were formulated as a cocktail containing equal molar concentrations of each

HIV

protein/peptide at a final concentration of approximately 1mg protein/ml in physiological saline.

The HIV-MDP cocktail (0.5 ml) was emulsified in squalene (1.5 ml containing 0.05% Tween 80) and injected subcutaneously into 60 goats at 4 to 6 injection sites per goat. Booster immunizations were given at monthly intervals to achieve the desired antibody responses. Monthly serum samples were obtained from each goat preceding the monthly booster and tested by Western Blot analysis, HIV neutralization and enzyme immunoassay disclosed in Example 8.6. Once the desired antibody response was achieved, goats were plasmapheresed from the jugular vein employing a Baxter A 201-A401 Autophresis machine. The goat red blood cells were returned in physiological saline. The volume of plasma collected from each animal at each plasmapheresis was 350 ml 5.0 ml.

The Caprine immune plasma was fractionated with octanoic acid, after adjustment of the pH to 4.8 with hydrochloric acid. The mixture was centrifuged, filtered and passed through an activated charcoal filter to remove agglomerates and reduce the octanoic acid level to below 0.05-. The immunoglobulin (IgG) containing fraction was further purified by passage through a series of columns: 1. Sephadex G25 with 2 0mN phosphate buffer 2. Ion exchange with Wharman DE53 equilibrated in pnhosphate buffer 3. Sephadex G25 equilibrated in 0.9% saline The final column filtrate was sterile filtered and then either concentrated or diluted to give a final concentration of 10 mg IgG/ml with the desired biological activity. SDS-PAGE and Western Blot analysis demonstrated reactivity of caprine IgG irmunoglobulins at a 1:100 dilution with the seven distinct viral proteins as gpl20, gp4l, p51/66, p24, p17, p7 and p!0. Analysis for HIV neutralization demonstrated broad neutralization of HIVI laboratory and wild strains as disclosed in Example 8.3. Analysis by the enzyme linked immunoassay disclosed in Example 8.3 demonstrated positive reactions to the desired nine epitopes at sample dilutions of 1:1000 or greater.

Production lots meeting these criteria were given the trade name of HRG214 as disclosed and consisted of sterile, pyrogen free caprine IgG at a concentration of 10mg/ml suspended in 0.9% sodium chloride without excipients. Each production lot is compared to the reference lot as disclosed herein to determine equivalence of HRG214 activity. Potency is expressed as mg equivalent/mi.

Males and non pregnant females over the age of 18 were selected based on their having a serodiagnosis of HIV infection documented by Western Blot, with AIDS defining criteria, clinically symptomatic, and with a CD4 T lymphocyte count 300 cells/ul within 30 days prior to study entry. Clinical and laboratory parameters were used to evaluate efficacy. Clinical parameters included changes in opportunistic infections, changes in body weight, changes in gastrointestinal function, including stool consistency and frequency, changes in energy level, changes in appetite, physical strength and endurance, and an overall change in the quality of life. Laboratory parameters included changes in CD4 and CD8 lymphocyte numbers, selected hematology, blood chemistry and urinalysis, and, when available, changes in viral loads by measuring viral RNA by

PCR.

The study results showed that with the use of the immunochemica!!y designed antibodies patients improved clinically, with decreases in opportunistic infections, increases in body weight, changes in gastrointestinal function, including less severe diarrhea, increases in energy level, increases in appetite, improvements in physical Strength and endurance, and an overall improvement in the quality of life. Laboratory parameters showed improvements, with increases in CD4 and CD8 lymphocyte numhemistrybers, improvements in hematology, blood chemistry, and urinalysis numbers, as well as decreases in viral loads by measuring viral RNA by

PCR

and decreases in infectivity when measured by

TCID.

The detailed laboratory results of the study are set forth in the attached Tables 9.1 9.13: Clinical Evaluation of the Toxicity and Efficacy of HRG214 in Treating HIV-Infected Patients Thirt-fG214 e H infected patients were treated with HRG214. HRG214 was evaluated for efficacy in reducing HIV viral burden and ameliorating disease progressr/on and symptos. Patients were stratified by CD4 number/mm3 (<200 and 200) and therapeutic regimen: Group 1: HRG214, CD4<200, n=ll; Group 2: HRG214 and monthly retreatment, CD4<20 0 Group 3: HRG24 and retreatment at progression, CD4<200, Group 4: HRG214, CD4>2 00 n=7; Group 5: HRG214 and retreatment at progression (patients receiving chemotherapy for malignancy) CD4<200, n=6; -Group 6: Placebo control, CD4>500 n=19; Group 7: Placebo control, CD4>200- 500 n=3. Patients in treatment groups received 21-28 daily infusions of HRG214 at 1-15.mg/kg body weight.

Patients with CD4 <200 5 mg/kg body weight. aents with CD4 <200 were either retreated x3 days monthly or retreated with evidence of HIV progression Adverse reactions were limited to minor fever of 2oF, chills, headache, and muscle ache. Clinical chemistry and hematology measurements during and after treatment remained unchanged or improved in all patients over a 90 day period. Twentyeight patients were evaluated for change in nutritional status. Twenty-four gained 2-22 lbs. body weight with a mean increase of 4.4 lbs. (P=0.0014) Four dy g h t f the 1 0 six patients receiving systemic chemotherapy for malignancy remained stable or lost weight (2 and 6 lbs respectively). Weight increases correlated directly with increases in serum total protein and albumin measurements. Quantitative HIV-RNA decreased in all treatment groups.

Group 2 HRG214 8 4714 4 Day 345, n= 6, S=64418 4713 9% Group 3 HRG224+ Day 469, n= 5, 25/67 705/856 -67% Group 4 HRG21431/8 97164- Day 405, n= 7, S=7 31/8 7/14 -94% Group 5 HRG214+ 43/41 476/592 -54% Day 469., n= 6r S=2 Chemo Group 6 control90/2NAA Day 469, n=l99962

AN

Group 7 Control Day 469, n=3 31S/177 NA

NA

The rate Of CD4/mm3 loss with time in all treatment glrou~ was reduced -o<0 01) compared to control groups 6 and 7. A sustained CD4 increase was observed in groups 2, 3 and 4. Infectivity measurements by microcujjture (TCID) demonstrated a 2 log reduction in infectivity by treatment day 7-14 (P<0.001) which was niot obvious from quantitative HIV-RNA measurements.

Clinical changes intCluded increases in appetite and st-am-ina with marked irnprove-ments in chronic fatigue syndrome, diarrhea, malabsorotion, candidiasis,

CMV

(retinitis excluded), Herpes simplex and zoster, cutaneous Molluscum contagiosum, oral hairy lekpI a watn yndrome, bacterial folliculitis' and pneumonitis and HIV related peripheral neuropathy were observed.

HRG214 offers a new drug to assist in the management of HIV infectionl.

100 Data for patient groups 2-5 are presented in more detail below: Patient Group 2 Patient n 6 H Primary Objective: Evaluate safety and efficacy of HRG214 treatment of HIV infection at a daily dose of mg/kg/day for 28 days and monthly retreatment x3.

Clinical follow-up will continue for 3 years. Patients will have retreat options with recurrence.

End Points: Normalization of clinical and laboratory parameters including improvement in opportunistic infections, incidence of infections, wasting syndrome, peripheral neuropathy and improvement in abnormal blood chemistry and hematology, CD4 and CD8 and reductions in HIV-RNA quantitated by PCR.

Safety variables include: Blood chemistry and hematology and clinical parameters.

Efficacy variable include: HIV-RNA Quantitative by PCR, CD4 and CD8 counts.

Follow up period 3 years Follow-up period to date >345 days Study Demographics: Patient n=6; Start Date 10/23/95; As of day 390, Survivors=6; Deaths; Lost to follow up=0.

Patient Demographics: HIV positive, AIDS defining criteria with CD4 number <50/mm Treatment Drug(s): HRG-21 4 1.5mg/kg/day with monthly retreatments.

101 Table 9.2 Patient Group 2 HIV-RNA QUANTITATIVE

BI

TEST DAY 1 DAY

DAY

21-28 60-90 Mean 4918.3 2092.5 1338 Std error 2422.6 1222 927.2 maximum 10649 4880 3993 Minimum 494 23 19 Median 42E5 1733.5 670 Table 9. 3 Patient Group 2 QUANTITATIVE CD4 /MM 3 TEST DAY 1 DAY

DAY

21-28 60-90 Mean 4452.8 52.8 Std error 14.2 11 1.

Maximum 72 96 82 Minimum 5 4 Median 49. 5 5.5 62 Table 9. 4 Patient Group 2 1 102 Patient Group 3 Patient n Primary Objective: Evaluate safety and efficacy of H-RG214 treatment of HIV infection at a daily dose of 1.5 mg/kg/day for 28 days. Clinical follow-up will continue for 3 years. Patients will have retreat options with recurrence.

End Points: Normalization of clinical and laboratory Parameters including improvement in opportunistic infections, incidence of infections, wasting syndrome, peripheral neuropathy and improvement in abnormal blood chemistry and hematology, CD4 and CD8 and reductions in HIV-RNA quantitated by

POR.

is Safety variables include: Blood chemistry and hematology and clinical parameters.

Efficacy variable include: HIV-RNA Quantitative by PCR, CD4 and CD8 counts.

Follow up Period year Follow-up period to date =>469 days Start date of 6/13/95; as of day 380r Survivors 3; Deaths Lost to follow up 0 Patient Population: HIV Positive patients with

AIDS

defining criteria. Five of the five Patients had CD4 <50/rn& blood. HIV-RNA quantitated by

POR

demonstrated statistically significant reductions following treatment; day 7 (P 0.0179) and days 21-28 (P=0.043). HIV RNA measurements on days 60-90 and 120- 150 demonstrated reduced but increasing HIV RNA values.

All .5 Patients were retreated (three consecutive doses monthly starting between days 120-150) Following re- treatment a statistically significant (P=0.0006) fall in HIV RNA measurements were observed by Day >250.

103 Statistical analysis was performed using paired t-Test, Wilcoxon Signed Rank Test and Mann-Whitney Rank Sum Test.

START DATE: 06/13/95 END DATE: Open Table Patient Group 2 "IV-RNA QUANTITATIVE BY PCR TEST DAY I DAY 7 DAY DAY Day DayDA 21-28 60-90 180-210 240-300 >8 Mean 21911 10642.2 10962.6 15231.8 18424 9385.2 81 Std 5003.7 3244.3 2222.5 7385.9 5473.5 5436.3 53 Median 187I37 150 68 65 7842 Table 9. 6 Patient Group 3 QUANTITATIVE CD4/,,n DAY 1 DAY 7 DAY DAY Day Day

DAY

21-28 60-90 180-210 24 0 _3 on 104 Table 9.7 Patient Group 3 QUANTITATIVE CD8/mm 3 TEST DAY 1 DAY 7 DAY DAY Day Day 21-28 60-90 180-210 240-300 Mean 705.6 633.8 486.4 681.4 570.8 826.6 Std 137.8 168.2 86 229 120.6 200.9 error 120.6 200.9 Maximm 1152 1037 700 1380 756 Minimum 444 220 260 180 i 10 Median 585 82810119 16 Median 585 828 540 720 638 812 8 Patient Group 4 Patient n 7 Primary Objective: Evaluate toxicity and efficacy of HRG214 treatment of HIV infection at a daily dose of 1.5 g/kg/day for 28 days with IFN inducer on days 1-7 and 21-23. Clinical follow-up will continue for 3 years. Patients will have retreat options with recurrence.

End Points: Normalization of clinical parameters and laboratory parameters including improvement in OI, incidence of infections, wasting syndrome, etc., in abnormal blood chemistry and hematology, CD4 and CD8, reductions in HIV-RNA quantitated by PCR.

Follow-up period years Follow-up period to date >405 days Study Demographics: Patient n=7; Start Date 8/25/95; As of day 390, Survivors=7; Deaths=0; Lost to Patient Demographics:

HIV

Patient Demoraphis thout HIV positive patients with CD4 number >200 without AIDS defining criterion Treatment Drug(s): 1.5 mg/kg/day for 28 days. Patients will have retreat options with recurrence.

105 Table 9. 8 Patient Group 4 HIV-RNA QUANTITATIVE

B

TEST DAY 1 DAY

DAY

21-28 60-90 Mean 607.8.7 964.3 899.7 Std error 949.4 639.6 199.1 Maximum 7936 2207 1257 Minimum 4808 80 569 Median 5492 606 873 Table 9. 9 Patient Group 4 106 Table 9.10 Patient Group 4 QUANTITATIVE CDS/mm 3 TEST DAY 1 DAY DAY DAY

DAY

21-28 60-90 180-210 330-39 S Mean 970.7 867.7 1016 1094 Std error 247.7 1164 Std error 247.7 158.7 378.8 229.5 235.2 Patient Group Patient n 6 Primary Objective: Evaluate toxicity and efficac of HRG214 treatment. Clinical follow-up will continue for 3 years. Patients will have retreat options with recurrence.

End Points: Normalization of clinical parameters and laboratory parameters including improvement in 01, incidence of infections, wasting syndrome, etc in abnormal blood chemistry and hematology, CD4 and CD8, reductions in

HV-RN

A quantitated by PCR Follow-up period 3 years Follow-up period to date 469 days SDeStart date of /13/95 as of day 270, Survivors S Deaths 4 (2 deas between 180-210, 1 death between 240-7; one death after 270); Lost to follow Patient Population: positive patients with

AIDS

defining criteria including CD4<20 0 m' blood and disseminated Kaposi's sarcoma Patients were treated with systemic chemotherapy. Two patients di e r e t eat e d days 180-210 and tw p patents died after day 240.

b e t w e e n and two patients died after day 240.

107 HIV-RNA quantitated by PCR demonstrated reductions at days 60-90, 120-150, 180-210 and 240-270 (p=0.018).

Statistical analysis was performed using paired t Test Wilcox, Signed Rank Test and Mann-Whitney Rank Sum Test.

Table 9.11 Patient Group Table 9.12 Patient Group 108 Table 9. 13 Patient Group QUANTITATIVE CD8/mm 3 TEST DAY I DAY DAY Day 21-28 60-90 120-15 Mean 476.3 303.5 415.8 371 Std 152.9 62.7 159.1 100.7 error Maximum 1170 540 1152 710 Minimum 98 86 92 62 Median r 109 BibliocraDhy 1. H. Mitsuya, S. Broder, Nature 325, 773-778 (1987).

2. The Molecular Biology of Tumor Viruses, J. Tooze, et al., Eds. (1973).

3. RNA Tumor Viruses. R. Weiss, Ed. (1982).

4. F. Gonzalez-Scarano, R.E. Shoppe, C.E. Calisher, N. Nathanson, Virology 120, 42-53 (1982).

S. Matsuno, S. Inouye, Infection and Immunity 39, 155-158 (1983).

6. J. Mathews, J. Roehrig, The Journal of Immunology 129, 2763-2767 (1982).

7. M. Robert-Guroff, M. Brown, R. Gallo, Nature 316, 72-74 (1985).

8. R. Weiss, et al., Nature 316, 69-72 (1985).

9. T. Matthews, et al., Proceedings of the National Academy of Sciences 83, 9709-9713 (1986).

W. Robey, et al., Proceedings of the National Academy of Sciences 83, 7023-7027 (1986).

11. L. Lasky, et al., Science 233, 209-212 (1986).

12. D. Zagury, et al., Nature 326, 249-250 (1987).

13. J. McDougal, et al., Science 231, 382-385 (1986).

14. S. Putney, et al., Science 234, 1392-13905 (1986).

S. Norley, R. Kurth, The Retroviridae J. Levy, Ed. (Plenum Press, 1994), vol. 16. J. Carlson, JAMA 260, 674-679 (1988).

17. J. Lange, et al., British Medical Journal 292, 228-230 (1986) 18. J. McDougal, et al., Journal of Clinical investigation 80, 316-324 (1987).

110 19. A. Amadori, A. De Rossi, G. Faulker-Valle, 1.

Chieco-Bianchi, Clini Cal Immunology and lImmunopathology 46, 342-351 (1988).

A. Amardori, et al., The Journal off Immunology 8.9, 2146-2152 (1989).

21. E. Barker, S. W. Barnett, L. Starnataos, J. A.

Levy, in The Viruses: The Retroviridae J.A. Levy, Ed. (Plenum Press, New York and London, 1995), vol. 4, pp. 1-7.

22. J. A. Levy, in HIV and the Pathogenesis off AIDS

A.

Press, Ed. (ASM Press, Washington, DC, 1994) pp.

23. D. F. Nixon, K. Broliden, G. Ogg, Broliden, Immnunology 76, 515-534 (1.992).

is 24. P. Linsley, J. Ledbetter, E. Kinney-Thomas,

S.-L

H-u, J Virology 62, 3695-3702 (1988).

M. Thali, et al., J Virology 66, 5635-5641 (1992).

26. A. Benjouard, J. Gluckmna, Rochat,

L.

Nontagnier, E. Bahraoui, J. Virology 66, 2473-83 (1992).

27. T. Chanh, et al., The EMBO Journal 5, 3065-71 (1986).

28 j. liorsy, M. Meyer, J. Levy, J. Virology 64, 1437- (1990) 29.. M. Tremblay, et al. J Immunology 145, 2896-2 901 (1990).

R. Garry, Science 250, 1127-1129 (1990).

M1 Mackett, G. Smith, B. Moss, Proc. Natl. Acad.

Sci. USA 79, 7415-7419 (1982).

32. D. Panicali, E. Paoietti, Proc. Natl. Acad. 3di.

USA 79, 4927-4931 (1982).

33. S. Hur, S. Kosowski, J. Daryrnple, Nature 320, 537-540 (1986).

34. S. Chakrabarti, M. Robert-Guroff, F. Wong-Staal, R. Gallo, B. Moss, Mature 320, 535-537 (1986).

I11 D. G. Kleid, et al., Science 214, 1125-1.129 (1981) 36. C. Cabradi.±a, et al., Bio/Technology 4, 128-133 (1986).

37. Current~ Protocols in Im unology (John Wiley Sons, 1995).

38. Remington's Pharmaceutical science (Mack Publishing Co, Easton, PA, ed. 15th, 1990).

39. B. Karpovsky, J. Titus, D. Stephany, D. Segal, Journal of Experimental Medicine 160, 1686-1701 (1984).

P..Cuatrecasas, Advances in Enzymology 36, 29 (1972).

41. P. Tijssen, Practice and Theory of EnzymeI Immunoassay (1985).

42. Stewart, Young, Solid Phase Peptide Synthesis (Pierce Chemical Co, edi. 2nd, 1984).

43. J. Tam, et al. Journal American Chemical Society 105, 6442 (1983).

44. Maniatis, Molecular Cloning: A Laboratory Manual (Cold Spring Harbor Laboratory, 1982).

Z. Grabarek, j. Gergely, Analytical Biochemistry 185 (1990).

46. J. Staros, R. Wright, D. Swingle, Analytical Biochemistry 1596, 220-222 (186).

47. R. Timkovich, Analytca Biochemistry2 314 (1977). yia 9 3 4 48. F. Gelder, et al. Annals o1' Surgery, 591-599 (1991).

419. Fung, et al., j. Virology, 66, 848-56 '(1992)