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AU2003246804A1 - Method and apparatus for extracting nucleic acids from a complex mixture - Google Patents

Method and apparatus for extracting nucleic acids from a complex mixture

Info

Publication number
AU2003246804A1
AU2003246804A1 AU2003246804A AU2003246804A AU2003246804A1 AU 2003246804 A1 AU2003246804 A1 AU 2003246804A1 AU 2003246804 A AU2003246804 A AU 2003246804A AU 2003246804 A AU2003246804 A AU 2003246804A AU 2003246804 A1 AU2003246804 A1 AU 2003246804A1
Authority
AU
Australia
Prior art keywords
nucleic acids
complex mixture
microorganisms
mixture
extracting nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2003246804A
Inventor
Xavier Badiche
Nadine Batisse-Debitte
Gabriel Festoc
Sylvie Hallier-Soulier
Gaelle Leborgne
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pall Genedisc Technologies SAS
Original Assignee
GeneSystems SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by GeneSystems SA filed Critical GeneSystems SA
Publication of AU2003246804A1 publication Critical patent/AU2003246804A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1017—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by filtration, e.g. using filters, frits, membranes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/12—Purification

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Sustainable Development (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Cell Biology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to a method for extracting nucleic acids of microorganisms contained in a complex mixture, particularly a food mixture, comprising at least the following steps: the mixture (10) is clarified; the microorganisms are retained on a filtering cartridge (12); said microorganisms are lysed (14); the nucleic acids are recovered (16), concentrated, and purified (18). The invention also relates to a filtering cartridge and an apparatus used for carrying out the inventive extraction method. Also disclosed is a device for extracting, detecting, and if necessary quantifying the nucleic acids of microorganisms contained in a complex mixture.
AU2003246804A 2002-04-09 2003-04-09 Method and apparatus for extracting nucleic acids from a complex mixture Abandoned AU2003246804A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR02/04424 2002-04-09
FR0204424A FR2838066B1 (en) 2002-04-09 2002-04-09 METHOD AND AUTOMATE FOR EXTRACTING NUCLEIC ACIDS FROM A COMPLEX MIXTURE
PCT/FR2003/001123 WO2003085109A2 (en) 2002-04-09 2003-04-09 Method and apparatus for extracting nucleic acids from a complex mixture

Publications (1)

Publication Number Publication Date
AU2003246804A1 true AU2003246804A1 (en) 2003-10-20

Family

ID=28052243

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2003246804A Abandoned AU2003246804A1 (en) 2002-04-09 2003-04-09 Method and apparatus for extracting nucleic acids from a complex mixture

Country Status (10)

Country Link
EP (1) EP1495119B1 (en)
JP (1) JP2005526509A (en)
AT (1) ATE351903T1 (en)
AU (1) AU2003246804A1 (en)
DE (1) DE60311244T2 (en)
DK (1) DK1495119T3 (en)
ES (1) ES2279960T3 (en)
FR (1) FR2838066B1 (en)
PT (1) PT1495119E (en)
WO (1) WO2003085109A2 (en)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2829500B1 (en) 2001-09-13 2003-12-12 Hemosystem PROCESS FOR THE CONCENTRATION AND DETECTION OF PATHOGENIC SPROUTS FROM BLOOD PRODUCTS AND / OR DERIVATIVES THEREOF AND DEVICE FOR CARRYING OUT SAID METHOD
US20050250138A1 (en) 2004-05-04 2005-11-10 Millipore Corporation Process for the enumeration and identification of microorganisms
JP4195039B2 (en) * 2006-02-16 2008-12-10 善之 辻本 Sample pretreatment method and reagent
FR2901281B1 (en) * 2006-05-19 2008-08-15 Hemosystem Sa PROCESS FOR EXTRACTING DESOXYRIBONUCLEIC ACIDS (DNA) FROM MICROORGANISMS WHICH MAY BE PRESENT IN A BLOOD SAMPLE
JP2008005781A (en) * 2006-06-29 2008-01-17 Olympus Corp Specimen-treating method
KR101702154B1 (en) 2009-03-24 2017-02-03 유니버시티 오브 시카고 Device for carrying out a reaction
US9464319B2 (en) 2009-03-24 2016-10-11 California Institute Of Technology Multivolume devices, kits and related methods for quantification of nucleic acids and other analytes
US10196700B2 (en) 2009-03-24 2019-02-05 University Of Chicago Multivolume devices, kits and related methods for quantification and detection of nucleic acids and other analytes
US9447461B2 (en) 2009-03-24 2016-09-20 California Institute Of Technology Analysis devices, kits, and related methods for digital quantification of nucleic acids and other analytes
KR102458821B1 (en) * 2015-12-29 2022-10-25 국민대학교산학협력단 A method for rapidly detecting microorganism using pcr product

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3788082T2 (en) * 1986-04-11 1994-06-09 Applied Biosystems Automatic device for extracting nucleic acids.
US5187083A (en) * 1990-11-13 1993-02-16 Specialty Laboratories, Inc. Rapid purification of DNA
FR2678950B1 (en) * 1991-07-09 1993-11-05 Bertin Et Cie CARTRIDGE, DEVICE AND METHOD FOR EXTRACTING NUCLEIC ACIDS SUCH AS DNA FROM A SAMPLE OF BLOOD OR TISSUE CELLS.
AU6104994A (en) * 1993-03-18 1994-10-11 Her Majesty The Queen In Right Of Canada As Represented By The Minister Of Agriculture Apparatus for the quantitative determination of particulate analytes
FR2712511B1 (en) * 1993-11-16 1996-02-09 Bertin & Cie Cartridge for the preparation of purified nucleic acids.
AU1752799A (en) * 1997-08-27 1999-04-05 Anda Biologicals S.A. Rapid filter diagnostic device and method for the direct detection of micro-organisms in body fluids
EP1119576B1 (en) * 1998-10-09 2003-06-11 Whatman Bioscience Limited Isolation method for nucleic acid and apparatus
US6958392B2 (en) * 1998-10-09 2005-10-25 Whatman, Inc. Methods for the isolation of nucleic acids and for quantitative DNA extraction and detection for leukocyte evaluation in blood products

Also Published As

Publication number Publication date
EP1495119A2 (en) 2005-01-12
PT1495119E (en) 2007-04-30
WO2003085109A2 (en) 2003-10-16
DK1495119T3 (en) 2007-05-21
ATE351903T1 (en) 2007-02-15
FR2838066B1 (en) 2004-06-25
ES2279960T3 (en) 2007-09-01
FR2838066A1 (en) 2003-10-10
WO2003085109A3 (en) 2004-04-01
JP2005526509A (en) 2005-09-08
EP1495119B1 (en) 2007-01-17
DE60311244D1 (en) 2007-03-08
DE60311244T2 (en) 2007-11-15

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Legal Events

Date Code Title Description
MK6 Application lapsed section 142(2)(f)/reg. 8.3(3) - pct applic. not entering national phase