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AU2001234992A1 - Methods for generating hypermutable bacteria - Google Patents

Methods for generating hypermutable bacteria

Info

Publication number
AU2001234992A1
AU2001234992A1 AU2001234992A AU3499201A AU2001234992A1 AU 2001234992 A1 AU2001234992 A1 AU 2001234992A1 AU 2001234992 A AU2001234992 A AU 2001234992A AU 3499201 A AU3499201 A AU 3499201A AU 2001234992 A1 AU2001234992 A1 AU 2001234992A1
Authority
AU
Australia
Prior art keywords
bacteria
methods
mismatch repair
generating hypermutable
desirable output
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001234992A
Inventor
Luigi Grasso
Kenneth W. Kinzler
Nicholas C. Nicolaides
Philip M. Sass
Bert Vogelstein
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Johns Hopkins University
Original Assignee
Johns Hopkins University
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Johns Hopkins University filed Critical Johns Hopkins University
Publication of AU2001234992A1 publication Critical patent/AU2001234992A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C12N15/1024—In vivo mutagenesis using high mutation rate "mutator" host strains by inserting genetic material, e.g. encoding an error prone polymerase, disrupting a gene for mismatch repair
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/01—Preparation of mutants without inserting foreign genetic material therein; Screening processes therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Bacteria are manipulated to create desirable output traits using dominant negative alleles of mismatch repair proteins. Enhanced hypermutation is achieved by combination of mismatch repair deficiency and exogenously applied mutagens. Stable bacteria containing desirable output traits are obtained by restoring mismatch repair activity to the bacteria.
AU2001234992A 2000-02-11 2001-02-12 Methods for generating hypermutable bacteria Abandoned AU2001234992A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US18192900P 2000-02-11 2000-02-11
US60/181,929 2000-02-11
PCT/US2001/004339 WO2001059092A2 (en) 2000-02-11 2001-02-12 Methods for generating hypermutable bacteria

Publications (1)

Publication Number Publication Date
AU2001234992A1 true AU2001234992A1 (en) 2001-08-20

Family

ID=22666395

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001234992A Abandoned AU2001234992A1 (en) 2000-02-11 2001-02-12 Methods for generating hypermutable bacteria

Country Status (7)

Country Link
US (3) US7026119B2 (en)
EP (1) EP1268765B1 (en)
AT (1) ATE404665T1 (en)
AU (1) AU2001234992A1 (en)
CA (1) CA2398094C (en)
DE (1) DE60135312D1 (en)
WO (1) WO2001059092A2 (en)

Families Citing this family (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6365410B1 (en) * 1999-05-19 2002-04-02 Genencor International, Inc. Directed evolution of microorganisms
ATE404665T1 (en) * 2000-02-11 2008-08-15 Univ Johns Hopkins METHOD FOR PRODUCING HYPERMUTATING BACTERIA
US6825038B2 (en) 2000-05-12 2004-11-30 The Johns Hopkins University Method for generating hypermutable organisms
US6737268B1 (en) * 2000-11-14 2004-05-18 Morphotek, Inc. Method for generating genetically altered antigens
US7638334B2 (en) * 2002-01-18 2009-12-29 Morphotek, Inc. Method for generating engineered cells by homologously recombining segments having increased degeneracy
AU2003248361B2 (en) * 2002-02-21 2008-07-03 Morphotek, Inc. Methods of making hypermutable cells using PMSR homologs
WO2003074655A2 (en) * 2002-02-21 2003-09-12 Morphotek, Inc. Regulated vectors for controlling dna hypermutability in eukariotic cells
US7754450B2 (en) 2002-11-15 2010-07-13 Morphotek, Inc. Methods of generating high-production of antibodies from hybridomas created by in vitro immunization
WO2006032504A2 (en) * 2004-09-22 2006-03-30 Vrije Universiteit Brussel Method for introducing genetic mutations into plant cells
US20090068741A1 (en) * 2007-01-17 2009-03-12 Invitrogen Corporation Methods and Compositions for Improving the Health of Cells in Culture
US8679845B2 (en) 2007-05-31 2014-03-25 University Of Washington B cells modified to reversibly induce accelerated mutagenesis of target genes
CN102911962B (en) * 2011-08-02 2017-03-22 中国科学院微生物研究所 Method for modifying microbes and for acquiring improved phenotype
AU2013232114B2 (en) 2012-03-15 2018-10-18 Omeros Corporation Composition and method for diversification of target sequences
CN108753814B (en) * 2018-06-11 2022-10-18 北京师范大学 Novel breeding method for accelerating species mutation

Family Cites Families (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6191268B1 (en) 1993-12-17 2001-02-20 Dana-Farber Cancer Institute Compositions and methods relating to DNA mismatch repair genes
US5843757A (en) 1995-08-24 1998-12-01 The Johns Hopkins University Human JTV1 gene overlaps PMS2 gene
US5907079A (en) 1996-01-18 1999-05-25 Amgen Canada Inc. MSH2 disrupted mice develop lymphomas
US6326204B1 (en) 1997-01-17 2001-12-04 Maxygen, Inc. Evolution of whole cells and organisms by recursive sequence recombination
AUPO974597A0 (en) 1997-10-10 1997-11-06 Rhone-Poulenc Agro Methods for obtaining plant varieties
US6146894A (en) * 1998-04-14 2000-11-14 The Johns Hopkins University Method for generating hypermutable organisms
ATE404665T1 (en) * 2000-02-11 2008-08-15 Univ Johns Hopkins METHOD FOR PRODUCING HYPERMUTATING BACTERIA
US6656736B2 (en) * 2000-02-23 2003-12-02 The Johns Hopkins University Methods for generating hypermutable yeast
US6737268B1 (en) * 2000-11-14 2004-05-18 Morphotek, Inc. Method for generating genetically altered antigens

Also Published As

Publication number Publication date
US20080318321A1 (en) 2008-12-25
WO2001059092A9 (en) 2002-10-24
US7695969B2 (en) 2010-04-13
WO2001059092A3 (en) 2002-07-25
CA2398094C (en) 2013-01-08
ATE404665T1 (en) 2008-08-15
DE60135312D1 (en) 2008-09-25
EP1268765B1 (en) 2008-08-13
WO2001059092A2 (en) 2001-08-16
US20020068284A1 (en) 2002-06-06
US7026119B2 (en) 2006-04-11
EP1268765A2 (en) 2003-01-02
US20060194236A1 (en) 2006-08-31
US7892832B2 (en) 2011-02-22
CA2398094A1 (en) 2001-08-16

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