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AU2001292749A1 - Twin-arginine translocation in bacillus - Google Patents

Twin-arginine translocation in bacillus

Info

Publication number
AU2001292749A1
AU2001292749A1 AU2001292749A AU9274901A AU2001292749A1 AU 2001292749 A1 AU2001292749 A1 AU 2001292749A1 AU 2001292749 A AU2001292749 A AU 2001292749A AU 9274901 A AU9274901 A AU 9274901A AU 2001292749 A1 AU2001292749 A1 AU 2001292749A1
Authority
AU
Australia
Prior art keywords
gram
host cell
bacillus
protein
secretion
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Abandoned
Application number
AU2001292749A
Inventor
Sierd Bron
Jan Douwe Harm Jongbloed
Jorg Paul Muller
Jan Maarten Van Dijl
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Publication of AU2001292749A1 publication Critical patent/AU2001292749A1/en
Abandoned legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/32—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Bacillus (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C12N15/625—DNA sequences coding for fusion proteins containing a sequence coding for a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/034—Fusion polypeptide containing a localisation/targetting motif containing a motif for targeting to the periplasmic space of Gram negative bacteria as a soluble protein, i.e. signal sequence should be cleaved

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Organic Low-Molecular-Weight Compounds And Preparation Thereof (AREA)
  • Cereal-Derived Products (AREA)

Abstract

Described herein are methods to enhance protein secretion in a host cell. In preferred embodiment, the host cell is a gram-positive microorganism such as a Bacillus. In another preferred embodiment, the host cell is a gram-negative microorganism. Preferably the gram-negative microorganism is an Escherichia coli or a member of the genus Pantoea. Protein secretion may be enhanced by the overexpression of protein components of the Tat pathway. Alternatively, secretion of foreign proteins can be selectively enhanced by forming a chimeric polypeptide comprising a tat signal sequence and the protein of interest. In a preferred embodiment, the tat signal sequence is selected from phoD or LipA.
AU2001292749A 2000-09-18 2001-09-17 Twin-arginine translocation in bacillus Abandoned AU2001292749A1 (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US23361000P 2000-09-18 2000-09-18
US60/233,610 2000-09-18
PCT/US2001/029151 WO2002022667A2 (en) 2000-09-18 2001-09-17 Twin-arginine translocation in bacillus

Publications (1)

Publication Number Publication Date
AU2001292749A1 true AU2001292749A1 (en) 2002-03-26

Family

ID=22877967

Family Applications (1)

Application Number Title Priority Date Filing Date
AU2001292749A Abandoned AU2001292749A1 (en) 2000-09-18 2001-09-17 Twin-arginine translocation in bacillus

Country Status (7)

Country Link
US (4) US7316924B2 (en)
EP (1) EP1356060B1 (en)
AT (1) ATE312932T1 (en)
AU (1) AU2001292749A1 (en)
DE (1) DE60115958T2 (en)
DK (1) DK1356060T3 (en)
WO (1) WO2002022667A2 (en)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7901693B2 (en) * 1998-09-30 2011-03-08 Corixa Corporation Compositions and methods for WT1 specific immunotherapy
US7655249B2 (en) * 1998-09-30 2010-02-02 Corixa Corporation Compositions and methods for WT1 specific immunotherapy
US7329410B1 (en) 1998-09-30 2008-02-12 Corixa Corporation Compositions and method for WT1 specific immunotherapy
US7553494B2 (en) 2001-08-24 2009-06-30 Corixa Corporation WT1 fusion proteins
EP1451367A4 (en) * 2001-11-05 2006-06-14 Res Dev Foundation Engineering of leader peptides for the secretion of recombinant proteins in bacteria
US7419783B2 (en) 2001-11-05 2008-09-02 Research Development Foundation Engineering of leader peptides for the secretion of recombinant proteins in bacteria
DE60310385T2 (en) * 2002-03-23 2007-09-20 Research Development Foundation, Carson City SECRETION OF PROTEINS WITH MULTIPLE DISULFIDE BINDINGS IN BACTERIA AND USES THEREOF
DE10256669B4 (en) * 2002-12-04 2006-03-09 Universitätsklinikum Charité an der Humboldt-Universität zu Berlin Technologietransferstelle Mixture of at least two fusion proteins and their preparation and use
EP1748077B1 (en) * 2004-04-20 2013-02-27 Ajinomoto Co., Inc. Method of producing protein
US20070026012A1 (en) * 2005-08-01 2007-02-01 Cornell Research Foundation, Inc. Compositions and methods for monitoring and altering protein folding and solubility
WO2007024877A2 (en) * 2005-08-22 2007-03-01 Cornell Research Foundation, Inc. Compositions and methods for analyzing protein interactions
KR100742218B1 (en) 2005-10-21 2007-07-24 학교법인 포항공과대학교 Secretion of target foreign protein in Escherichia coli using an organophosphorus hydrolase secretion sequence having a twin arginine translocation pathway
EP2426199A3 (en) 2006-10-20 2012-08-22 Danisco US Inc. Polyol oxidases
WO2008065200A1 (en) * 2006-11-30 2008-06-05 Novozymes A/S Dnase expression in recombinant host cells
WO2008089132A2 (en) * 2007-01-12 2008-07-24 Cornell Research Foundation, Inc. Genetic selection for protein folding and solubility in the bacterial periplasm
WO2008156551A2 (en) * 2007-06-18 2008-12-24 Danisco Us, Inc., Genencor Division Tat signal peptides for producing proteins in prokaryotes
DE102008025926A1 (en) 2008-05-29 2009-12-03 Henkel Ag & Co. Kgaa Secretion-optimized microorganism
DE102008025791A1 (en) 2008-05-29 2009-12-03 Henkel Ag & Co. Kgaa Secretion-optimized microorganism
HRP20200447T1 (en) * 2013-09-11 2020-06-12 Impossible Foods Inc. Secretion of heme-containing polypeptides
US9616114B1 (en) 2014-09-18 2017-04-11 David Gordon Bermudes Modified bacteria having improved pharmacokinetics and tumor colonization enhancing antitumor activity
EP3294762B1 (en) 2015-05-11 2022-01-19 Impossible Foods Inc. Expression constructs and methods of genetically engineering methylotrophic yeast
KR101791526B1 (en) * 2016-02-18 2017-11-01 (주)케어젠 Peptides having Hair Growth Activity and Uses Thereof
US11180535B1 (en) 2016-12-07 2021-11-23 David Gordon Bermudes Saccharide binding, tumor penetration, and cytotoxic antitumor chimeric peptides from therapeutic bacteria
US11129906B1 (en) 2016-12-07 2021-09-28 David Gordon Bermudes Chimeric protein toxins for expression by therapeutic bacteria
GB201713732D0 (en) * 2017-08-25 2017-10-11 Alta Innovations Ltd Tat expression system
US11471497B1 (en) 2019-03-13 2022-10-18 David Gordon Bermudes Copper chelation therapeutics
CN114341351A (en) 2019-04-25 2022-04-12 非凡食品有限公司 Strains and methods for producing hemoprotein
US10973908B1 (en) 2020-05-14 2021-04-13 David Gordon Bermudes Expression of SARS-CoV-2 spike protein receptor binding domain in attenuated salmonella as a vaccine

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Publication number Priority date Publication date Assignee Title
NL154598B (en) * 1970-11-10 1977-09-15 Organon Nv PROCEDURE FOR DETERMINING AND DETERMINING LOW MOLECULAR COMPOUNDS AND PROTEINS THAT CAN SPECIFICALLY BIND THESE COMPOUNDS AND TEST PACKAGING.
US3817837A (en) * 1971-05-14 1974-06-18 Syva Corp Enzyme amplification assay
US3939350A (en) * 1974-04-29 1976-02-17 Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing total reflection for activation
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US4277437A (en) * 1978-04-05 1981-07-07 Syva Company Kit for carrying out chemically induced fluorescence immunoassay
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US4816567A (en) * 1983-04-08 1989-03-28 Genentech, Inc. Recombinant immunoglobin preparations
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US5641671A (en) 1990-07-06 1997-06-24 Unilever Patent Holdings B.V. Production of active Pseudomonas glumae lipase in homologous or heterologous hosts
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EP1354056B1 (en) * 2000-10-10 2014-11-26 Danisco US Inc. Enhanced secretion of a polypeptide by a microorganism

Also Published As

Publication number Publication date
US7316924B2 (en) 2008-01-08
US7897742B2 (en) 2011-03-01
US20020110860A1 (en) 2002-08-15
DE60115958D1 (en) 2006-01-19
US20090093025A1 (en) 2009-04-09
DK1356060T3 (en) 2006-05-01
US7884191B2 (en) 2011-02-08
DE60115958T2 (en) 2006-08-03
ATE312932T1 (en) 2005-12-15
WO2002022667A9 (en) 2003-03-20
US20080166757A1 (en) 2008-07-10
EP1356060B1 (en) 2005-12-14
US7884192B2 (en) 2011-02-08
WO2002022667A3 (en) 2003-09-04
WO2002022667A2 (en) 2002-03-21
EP1356060A2 (en) 2003-10-29
US20080248525A1 (en) 2008-10-09

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