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ATE547518T1 - Verfahren zur sammlung von kristallinen partikeln aus fermentationsbrühe - Google Patents

Verfahren zur sammlung von kristallinen partikeln aus fermentationsbrühe

Info

Publication number
ATE547518T1
ATE547518T1 AT02781173T AT02781173T ATE547518T1 AT E547518 T1 ATE547518 T1 AT E547518T1 AT 02781173 T AT02781173 T AT 02781173T AT 02781173 T AT02781173 T AT 02781173T AT E547518 T1 ATE547518 T1 AT E547518T1
Authority
AT
Austria
Prior art keywords
fermentation broth
crystalline particles
collecting crystalline
collecting
crystalline
Prior art date
Application number
AT02781173T
Other languages
English (en)
Inventor
Benny Nielsen
Anders Rancke-Madsen
Martin Joergensen
Original Assignee
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Novozymes As filed Critical Novozymes As
Application granted granted Critical
Publication of ATE547518T1 publication Critical patent/ATE547518T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C12N9/2411—Amylases
    • C12N9/2414—Alpha-amylase (3.2.1.1.)
    • C12N9/2417—Alpha-amylase (3.2.1.1.) from microbiological source
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/30—Extraction; Separation; Purification by precipitation
    • C07K1/306—Extraction; Separation; Purification by precipitation by crystallization
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Mushroom Cultivation (AREA)
AT02781173T 2001-12-11 2002-12-04 Verfahren zur sammlung von kristallinen partikeln aus fermentationsbrühe ATE547518T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA200101847 2001-12-11
PCT/DK2002/000821 WO2003050274A2 (en) 2001-12-11 2002-12-04 Process for harvesting crystalline particles from fermentation broth

Publications (1)

Publication Number Publication Date
ATE547518T1 true ATE547518T1 (de) 2012-03-15

Family

ID=8160891

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02781173T ATE547518T1 (de) 2001-12-11 2002-12-04 Verfahren zur sammlung von kristallinen partikeln aus fermentationsbrühe

Country Status (6)

Country Link
EP (1) EP1456361B1 (de)
CN (1) CN100487114C (de)
AT (1) ATE547518T1 (de)
AU (1) AU2002349300A1 (de)
DK (1) DK1456361T3 (de)
WO (1) WO2003050274A2 (de)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1685256A1 (de) * 2003-10-31 2006-08-02 Novozymes A/S Verfahren zur ausflockung einer einen pilz enthaltenden fermentationsbrühe
EP1699811B1 (de) * 2003-12-30 2016-07-27 Bharat Biotech International Limited Verfahren zur herstellung und aufreinigung rekombinanter proteine
CN101679482A (zh) * 2006-12-22 2010-03-24 塔利克里斯生物治疗公司 用于沉淀肽的装置和方法
DE102008029401A1 (de) * 2008-06-23 2009-12-24 Bayer Technology Services Gmbh Verfahren zur Kristallisation von Peptiden und Proteinen
EP2196539B1 (de) * 2008-12-15 2022-02-09 Delft Advanced Biofuels B.V. Verfahren zur kontinuierlichen biologischen Herstellung von Lipiden, Kohlenwasserstoffen oder Zusammensetzungen davon
US8889395B2 (en) 2010-03-30 2014-11-18 Novozymes A/S Crystal metabolite recovery
CN102838552B (zh) * 2012-09-21 2015-08-05 江南大学 采用复合絮凝法生产高纯度吩嗪-1-羧酸的方法
WO2014068083A1 (en) 2012-11-01 2014-05-08 Novozymes A/S Method for removal of dna
CN106146610B (zh) * 2016-09-22 2018-01-23 济南大学 一种采用絮凝法制备谷胱甘肽亚铜盐的方法
EP3606936B1 (de) * 2017-04-03 2021-10-20 Novozymes A/S Verfahren zur rückgewinnung
US11597951B2 (en) 2018-02-13 2023-03-07 Lygos, Inc. Method for preparation of diester derivatives of malonic acid
CN113166231A (zh) 2018-10-10 2021-07-23 诺维信公司 胰凝乳蛋白酶抑制剂变体及其用途
WO2024032881A1 (en) * 2022-08-10 2024-02-15 Arla Foods Amba Crystallisation of a protein capable of crystallizing under salting-in conditions using multiple protein feeds

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4659667A (en) * 1985-02-26 1987-04-21 Miles Laboratories, Inc. Process to recover crystalline enzymes and crystalline enzymes produced thereby
JP2958789B2 (ja) * 1990-01-22 1999-10-06 味の素株式会社 アミノ酸・核酸およびその誘導体の精製方法
EP0574050A1 (de) * 1992-05-19 1993-12-15 Gist-Brocades N.V. Trennung und Reinigung von Fermentationsprodukt in grossen Massstab
DK83093D0 (da) * 1993-07-09 1993-07-09 Novo Nordisk As Fremgangsmaade
EP0828753B1 (de) * 1995-06-02 2003-03-26 Novozymes A/S BEHANDLUNG EINER PROTEINLÖSUNG MIT A1/Fe UND ANSCHLIESENDE MEMBRANKONZENTRATION

Also Published As

Publication number Publication date
CN1599795A (zh) 2005-03-23
AU2002349300A8 (en) 2003-06-23
CN100487114C (zh) 2009-05-13
WO2003050274A3 (en) 2003-12-11
DK1456361T3 (da) 2012-06-11
EP1456361A2 (de) 2004-09-15
EP1456361B1 (de) 2012-02-29
AU2002349300A1 (en) 2003-06-23
WO2003050274A2 (en) 2003-06-19

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