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ATE328069T1 - Phosphopantethenyltransferasen und deren verwendungen - Google Patents

Phosphopantethenyltransferasen und deren verwendungen

Info

Publication number
ATE328069T1
ATE328069T1 AT96936313T AT96936313T ATE328069T1 AT E328069 T1 ATE328069 T1 AT E328069T1 AT 96936313 T AT96936313 T AT 96936313T AT 96936313 T AT96936313 T AT 96936313T AT E328069 T1 ATE328069 T1 AT E328069T1
Authority
AT
Austria
Prior art keywords
transferases
phosphopantetheinyl
phosphopantetheinyl transferases
vitro
vivo
Prior art date
Application number
AT96936313T
Other languages
English (en)
Inventor
Ralph H Lambalot
Amy M Harvard Gehring
Ralph Reid
Christopher T Walsh
Original Assignee
Harvard College
Univ California San Francisco
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Harvard College, Univ California San Francisco filed Critical Harvard College
Application granted granted Critical
Publication of ATE328069T1 publication Critical patent/ATE328069T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1258—Polyribonucleotide nucleotidyltransferase (2.7.7.8), i.e. polynucleotide phosphorylase
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/04—Antibacterial agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/5005—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving human or animal cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Zoology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pathology (AREA)
  • General Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Communicable Diseases (AREA)
  • Oncology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Virology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT96936313T 1995-10-13 1996-10-11 Phosphopantethenyltransferasen und deren verwendungen ATE328069T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US515295P 1995-10-13 1995-10-13
US2165096P 1996-07-12 1996-07-12

Publications (1)

Publication Number Publication Date
ATE328069T1 true ATE328069T1 (de) 2006-06-15

Family

ID=26673994

Family Applications (1)

Application Number Title Priority Date Filing Date
AT96936313T ATE328069T1 (de) 1995-10-13 1996-10-11 Phosphopantethenyltransferasen und deren verwendungen

Country Status (8)

Country Link
US (2) US6579695B1 (de)
EP (1) EP0861321B1 (de)
JP (1) JP4709333B2 (de)
AT (1) ATE328069T1 (de)
AU (1) AU727348B2 (de)
CA (1) CA2232230C (de)
DE (1) DE69636195T2 (de)
WO (1) WO1997013845A2 (de)

Families Citing this family (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6558942B1 (en) 1994-05-06 2003-05-06 The Leland Stanford Junior University Combinatorial polyketide libraries produced using a modular PKS gene cluster as scaffold
US6066721A (en) * 1995-07-06 2000-05-23 Stanford University Method to produce novel polyketides
US6500960B1 (en) 1995-07-06 2002-12-31 Stanford University (Board Of Trustees Of The Leland Stanford Junior University) Method to produce novel polyketides
EP1493814A1 (de) 1995-07-06 2005-01-05 The Leland Stanford Junior University Zellfreie Synthese von Polyketiden
US6033883A (en) * 1996-12-18 2000-03-07 Kosan Biosciences, Inc. Production of polyketides in bacteria and yeast
US6902913B2 (en) 1997-04-30 2005-06-07 Kosan Biosciences, Inc. Recombinant narbonolide polyketide synthase
US6117659A (en) * 1997-04-30 2000-09-12 Kosan Biosciences, Inc. Recombinant narbonolide polyketide synthase
US6503741B1 (en) 1998-05-28 2003-01-07 Kosan Biosciences, Inc. Polyketide synthase genes from Streptomyces venezuelae
US6262340B1 (en) 1997-07-10 2001-07-17 Kosan Biosciences, Inc. Production of polyketides in plants
CA2347128A1 (en) 1998-10-28 2000-05-04 Kosan Biosciences, Inc. Library of novel "unnatural" natural products
US6410301B1 (en) 1998-11-20 2002-06-25 Kosan Biosciences, Inc. Myxococcus host cells for the production of epothilones
US6303342B1 (en) 1998-11-20 2001-10-16 Kason Biosciences, Inc. Recombinant methods and materials for producing epothilones C and D
ES2335385T3 (es) 1999-10-13 2010-03-26 The Board Of Trustees Of The Leland Stanford Junior University Biosintesis de sustratos de policetido sintasa.
US6939691B1 (en) * 1999-10-13 2005-09-06 Board Of Trustees Of The Leland Stanford Junior University E. coli and Streptomyces host cells that contain MatBC genes or E. coli host cells that contain pcc genes useful for enhanced polyketide production
DE60026563D1 (de) 1999-10-25 2006-05-04 Kosan Biosciences Inc Herstellung von polyketiden
ATE356216T1 (de) 1999-10-27 2007-03-15 Kosan Biosciences Inc Heterologe herstellung von polyketiden
CN1245215C (zh) * 2001-02-28 2006-03-15 四川省生物工程研究中心 重组高效复合干扰素用作乙型肝炎表面抗原和e抗原抑制剂
US20050079579A1 (en) * 2001-02-28 2005-04-14 Guangwen Wei Uses of spatial configuration to modulate protein function
US20060035327A1 (en) * 2001-02-28 2006-02-16 Guangwen Wei Recombinant super-compound interferon and uses thereof
JP2006514636A (ja) * 2002-12-02 2006-05-11 バイオシネクサス インコーポレーテッド 抗ブドウ球菌療法およびワクチンの標的としての壁テイコイン酸
NZ543653A (en) * 2003-05-23 2008-10-31 Epfl Ecole Polytechnique Federale Lausanne Methods for protein labeling based on acyl carrier protein
US7335496B2 (en) * 2003-06-05 2008-02-26 Ajinomoto Co., Inc. Method for producing target substance
CA2535982C (en) * 2003-08-28 2016-11-29 Huiyangtech (Usa), Inc. Uses of interferons with altered spatial structure
US7585647B2 (en) * 2003-08-28 2009-09-08 Guangwen Wei Nucleic acid encoding recombinant interferon
KR20150103335A (ko) * 2003-08-28 2015-09-09 수퍼랩 파 이스트 리미티드 변경된 공간 구조를 갖는 인터페론의 용도
CA2555737A1 (en) * 2004-02-13 2005-09-01 Martek Biosciences Corporation Schizochytrium fatty acid synthase (fas) and products and methods related thereto
WO2006060839A2 (en) * 2004-12-10 2006-06-15 Universität Für Bodenkultur Wien Method for identifying ppt inhibitors
US20080124302A1 (en) * 2005-03-09 2008-05-29 Guangwen Wei Uses of Recombinant Super-Compound Interferons
EP1795608A1 (de) * 2005-12-08 2007-06-13 Centre National de la Recherche Scientifique Verwendung einer 4' Phosphopantetheinyl-Transferase als Ziel bei Identifizierung antibiotischer Moleküle
WO2009092808A1 (en) * 2008-01-24 2009-07-30 National University Of Ireland, Maynooth Method for detecting phosphopantetheinyl transferase activity
WO2010127186A1 (en) 2009-04-30 2010-11-04 Prognosys Biosciences, Inc. Nucleic acid constructs and methods of use
WO2010135624A2 (en) 2009-05-22 2010-11-25 Codexis, Inc. Engineered biosynthesis of fatty alcohols
GB0909743D0 (en) * 2009-06-05 2009-07-22 F2G Ltd Antifungal targgt
AU2011237729B2 (en) 2010-04-05 2014-04-03 Prognosys Biosciences, Inc. Spatially encoded biological assays
US10787701B2 (en) 2010-04-05 2020-09-29 Prognosys Biosciences, Inc. Spatially encoded biological assays
US20190300945A1 (en) 2010-04-05 2019-10-03 Prognosys Biosciences, Inc. Spatially Encoded Biological Assays
EP3150750B1 (de) 2011-04-08 2018-12-26 Prognosys Biosciences, Inc. Peptidkonstrukte und testsysteme dafür
GB201106254D0 (en) 2011-04-13 2011-05-25 Frisen Jonas Method and product
KR102353113B1 (ko) * 2012-04-02 2022-01-19 게노마티카 인코포레이티드 지방산 유도체들의 개선된 생산
JP6321633B2 (ja) 2012-06-04 2018-05-09 ノバルティス アーゲー 部位特異的標識法およびそれによって生成される分子
WO2014043561A1 (en) * 2012-09-14 2014-03-20 The Regents Of The University Of California Reversible chemoenzymatic labeling of native and fusion carrier protein motifs
USRE50065E1 (en) 2012-10-17 2024-07-30 10X Genomics Sweden Ab Methods and product for optimising localised or spatial detection of gene expression in a tissue sample
TWI726291B (zh) 2013-01-07 2021-05-01 英屬維爾京群島商遠東超級實驗室有限公司 通過干擾素的經皮和/或經粘膜給藥治療骨癌、皮膚癌、皮下癌、粘膜癌和/或粘膜下癌的方法和組合物
CN111233978B (zh) 2013-03-15 2024-08-16 普罗格诺西斯生物科学公司 用于检测肽/mhc/tcr结合的方法
CN105849275B (zh) 2013-06-25 2020-03-17 普罗格诺西斯生物科学公司 检测样品中生物靶标的空间分布的方法和系统
WO2015070037A2 (en) 2013-11-08 2015-05-14 Prognosys Biosciences, Inc. Polynucleotide conjugates and methods for analyte detection
ES2935860T3 (es) 2015-04-10 2023-03-13 Spatial Transcriptomics Ab Análisis de ácidos nucleicos múltiplex, espacialmente distinguidos de especímenes biológicos
US11519033B2 (en) 2018-08-28 2022-12-06 10X Genomics, Inc. Method for transposase-mediated spatial tagging and analyzing genomic DNA in a biological sample
WO2020123320A2 (en) 2018-12-10 2020-06-18 10X Genomics, Inc. Imaging system hardware
US11649485B2 (en) 2019-01-06 2023-05-16 10X Genomics, Inc. Generating capture probes for spatial analysis
US11926867B2 (en) 2019-01-06 2024-03-12 10X Genomics, Inc. Generating capture probes for spatial analysis
WO2020243579A1 (en) 2019-05-30 2020-12-03 10X Genomics, Inc. Methods of detecting spatial heterogeneity of a biological sample
CN110596065B (zh) * 2019-09-25 2021-10-15 福建医科大学 基于半胱胺-n-乙酰-l-半胱氨酸-金纳米团簇荧光材料的酸性磷酸酶检测方法
EP4025711A2 (de) 2019-11-08 2022-07-13 10X Genomics, Inc. Erhöhung der spezifität einer analytbindung
WO2021091611A1 (en) 2019-11-08 2021-05-14 10X Genomics, Inc. Spatially-tagged analyte capture agents for analyte multiplexing
AU2020412766B2 (en) 2019-12-23 2024-09-19 10X Genomics, Inc. Methods for spatial analysis using RNA-templated ligation
US20210190770A1 (en) 2019-12-23 2021-06-24 10X Genomics, Inc. Compositions and methods for using fixed biological samples in partition-based assays
US11732299B2 (en) 2020-01-21 2023-08-22 10X Genomics, Inc. Spatial assays with perturbed cells
US11702693B2 (en) 2020-01-21 2023-07-18 10X Genomics, Inc. Methods for printing cells and generating arrays of barcoded cells
US12076701B2 (en) 2020-01-31 2024-09-03 10X Genomics, Inc. Capturing oligonucleotides in spatial transcriptomics
US12110541B2 (en) 2020-02-03 2024-10-08 10X Genomics, Inc. Methods for preparing high-resolution spatial arrays
US11898205B2 (en) 2020-02-03 2024-02-13 10X Genomics, Inc. Increasing capture efficiency of spatial assays
US11732300B2 (en) 2020-02-05 2023-08-22 10X Genomics, Inc. Increasing efficiency of spatial analysis in a biological sample
US12129516B2 (en) 2020-02-07 2024-10-29 10X Genomics, Inc. Quantitative and automated permeabilization performance evaluation for spatial transcriptomics
US11891654B2 (en) 2020-02-24 2024-02-06 10X Genomics, Inc. Methods of making gene expression libraries
WO2021216708A1 (en) 2020-04-22 2021-10-28 10X Genomics, Inc. Methods for spatial analysis using targeted rna depletion
EP4414459A3 (de) 2020-05-22 2024-09-18 10X Genomics, Inc. Simultane räumlich-zeitliche messung der genexpression und der zellaktivität
EP4153776A1 (de) 2020-05-22 2023-03-29 10X Genomics, Inc. Räumliche analyse zur erkennung von sequenzvarianten
WO2021242834A1 (en) 2020-05-26 2021-12-02 10X Genomics, Inc. Method for resetting an array
US12031177B1 (en) 2020-06-04 2024-07-09 10X Genomics, Inc. Methods of enhancing spatial resolution of transcripts
WO2021252499A1 (en) 2020-06-08 2021-12-16 10X Genomics, Inc. Methods of determining a surgical margin and methods of use thereof
EP4165207B1 (de) 2020-06-10 2024-09-25 10X Genomics, Inc. Verfahren zur bestimmung der position eines analyten in einer biologischen probe
EP4450639A2 (de) 2020-06-25 2024-10-23 10X Genomics, Inc. Räumliche analyse der dna-methylierung
US11981960B1 (en) 2020-07-06 2024-05-14 10X Genomics, Inc. Spatial analysis utilizing degradable hydrogels
US11761038B1 (en) 2020-07-06 2023-09-19 10X Genomics, Inc. Methods for identifying a location of an RNA in a biological sample
US11981958B1 (en) 2020-08-20 2024-05-14 10X Genomics, Inc. Methods for spatial analysis using DNA capture
US11926822B1 (en) 2020-09-23 2024-03-12 10X Genomics, Inc. Three-dimensional spatial analysis
US11827935B1 (en) 2020-11-19 2023-11-28 10X Genomics, Inc. Methods for spatial analysis using rolling circle amplification and detection probes
AU2021409136A1 (en) 2020-12-21 2023-06-29 10X Genomics, Inc. Methods, compositions, and systems for capturing probes and/or barcodes
EP4301870A1 (de) 2021-03-18 2024-01-10 10X Genomics, Inc. Multiplex-erfassung von gen- und proteinexpression aus einer biologischen probe
EP4347879A1 (de) 2021-06-03 2024-04-10 10X Genomics, Inc. Verfahren, zusammensetzungen, kits und systeme zur verbesserung der analyterfassung zur räumlichen analyse
WO2023034489A1 (en) 2021-09-01 2023-03-09 10X Genomics, Inc. Methods, compositions, and kits for blocking a capture probe on a spatial array

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2100791C (en) 1991-01-17 2000-05-30 Leonard Katz Method of directing biosynthesis of specific polyketides
US5824513A (en) 1991-01-17 1998-10-20 Abbott Laboratories Recombinant DNA method for producing erythromycin analogs
WO1993013663A1 (en) 1992-01-17 1993-07-22 Abbott Laboratories Method of directing biosynthesis of specific polyketides

Also Published As

Publication number Publication date
EP0861321A2 (de) 1998-09-02
US6579695B1 (en) 2003-06-17
WO1997013845A3 (en) 1997-06-26
JP4709333B2 (ja) 2011-06-22
CA2232230A1 (en) 1997-04-17
WO1997013845A2 (en) 1997-04-17
CA2232230C (en) 2005-06-28
DE69636195T2 (de) 2007-03-29
DE69636195D1 (de) 2006-07-06
US7192735B2 (en) 2007-03-20
AU7435796A (en) 1997-04-30
AU727348B2 (en) 2000-12-14
JPH11514868A (ja) 1999-12-21
EP0861321B1 (de) 2006-05-31
US20030138879A1 (en) 2003-07-24

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