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ATE327345T1 - Gel mikrotropfen für die genetische analyse - Google Patents

Gel mikrotropfen für die genetische analyse

Info

Publication number
ATE327345T1
ATE327345T1 AT99939044T AT99939044T ATE327345T1 AT E327345 T1 ATE327345 T1 AT E327345T1 AT 99939044 T AT99939044 T AT 99939044T AT 99939044 T AT99939044 T AT 99939044T AT E327345 T1 ATE327345 T1 AT E327345T1
Authority
AT
Austria
Prior art keywords
population
gel
nucleic acid
microdrops
genetic analysis
Prior art date
Application number
AT99939044T
Other languages
English (en)
Inventor
Jan Trnovsky
Patricia Mcgrath
Original Assignee
Cellay Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cellay Llc filed Critical Cellay Llc
Application granted granted Critical
Publication of ATE327345T1 publication Critical patent/ATE327345T1/de

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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6841In situ hybridisation
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
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    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/70Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving virus or bacteriophage
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    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/156Polymorphic or mutational markers
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    • C12Q2600/00Oligonucleotides characterized by their use
    • C12Q2600/158Expression markers

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • General Engineering & Computer Science (AREA)
  • Virology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Saccharide Compounds (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
AT99939044T 1998-08-07 1999-08-06 Gel mikrotropfen für die genetische analyse ATE327345T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US9572198P 1998-08-07 1998-08-07

Publications (1)

Publication Number Publication Date
ATE327345T1 true ATE327345T1 (de) 2006-06-15

Family

ID=22253300

Family Applications (1)

Application Number Title Priority Date Filing Date
AT99939044T ATE327345T1 (de) 1998-08-07 1999-08-06 Gel mikrotropfen für die genetische analyse

Country Status (8)

Country Link
US (5) US6586176B1 (de)
EP (1) EP1102864B1 (de)
JP (1) JP2002531056A (de)
AT (1) ATE327345T1 (de)
AU (1) AU764633B2 (de)
CA (1) CA2339734A1 (de)
DE (1) DE69931497T2 (de)
WO (1) WO2000008212A1 (de)

Families Citing this family (150)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP2002531056A (ja) * 1998-08-07 2002-09-24 セレイ, エルエルシー 遺伝子分析におけるゲルマイクロドロップ
JP2005501217A (ja) * 2000-10-10 2005-01-13 ディベルサ コーポレーション 生体活性または生体分子のハイスループットスクリーニングまたはキャピラリーに基づくスクリーニング
US8008459B2 (en) 2001-01-25 2011-08-30 Evolva Sa Concatemers of differentially expressed multiple genes
US7838287B2 (en) 2001-01-25 2010-11-23 Evolva Sa Library of a collection of cells
US20030124549A1 (en) * 2001-10-11 2003-07-03 Xerox Corporation Devices and methods for detecting genetic sequences
DE60321325D1 (de) * 2002-03-20 2008-07-10 Innovativebio Biz Kowloon Mikrokapseln mit kontrollierter durchlässigkeit die ein nukleinsäureamplifizierungsreaktionsgemisch enthalten und deren benutzung als reaktionsgefäss für parallele reaktionen
US20100022414A1 (en) 2008-07-18 2010-01-28 Raindance Technologies, Inc. Droplet Libraries
JP4797396B2 (ja) * 2004-02-19 2011-10-19 大日本印刷株式会社 細胞培養基板の製造方法
US7919305B2 (en) * 2004-02-19 2011-04-05 Dai Nippon Printing Co., Ltd. Method for manufacturing cell culture substrate
US7968287B2 (en) 2004-10-08 2011-06-28 Medical Research Council Harvard University In vitro evolution in microfluidic systems
JP4977325B2 (ja) * 2004-10-15 2012-07-18 シスメックス株式会社 精度管理用疑似組織及びそれを用いた精度管理方法
JP4822689B2 (ja) * 2004-10-29 2011-11-24 株式会社Kri 微生物単離装置、微生物の単離方法および該方法で得られた微生物含有微粒子
US9562837B2 (en) 2006-05-11 2017-02-07 Raindance Technologies, Inc. Systems for handling microfludic droplets
EP4190448A3 (de) 2006-05-11 2023-09-20 Bio-Rad Laboratories, Inc. Mikrofluidische vorrichtungen
WO2008097559A2 (en) 2007-02-06 2008-08-14 Brandeis University Manipulation of fluids and reactions in microfluidic systems
US9029085B2 (en) 2007-03-07 2015-05-12 President And Fellows Of Harvard College Assays and other reactions involving droplets
JP2008245612A (ja) * 2007-03-30 2008-10-16 Hitachi Ltd 試料調製法および装置
US8592221B2 (en) 2007-04-19 2013-11-26 Brandeis University Manipulation of fluids, fluid components and reactions in microfluidic systems
US20090068170A1 (en) * 2007-07-13 2009-03-12 President And Fellows Of Harvard College Droplet-based selection
EP2235210B1 (de) 2007-12-21 2015-03-25 President and Fellows of Harvard College Verfahren zur nukleinsäuresequenzierung
AU2009220203B2 (en) 2008-03-04 2014-02-20 Crystal Bioscience Inc. Gel microdrop composition and method of using the same
US20110124965A1 (en) * 2008-05-08 2011-05-26 Park Jason Y Chemiluminescence enhanced detection
US9297035B2 (en) 2008-05-27 2016-03-29 Dako Denmark A/S Compositions and methods for detection of chromosomal aberrations with novel hybridization buffers
US12038438B2 (en) 2008-07-18 2024-07-16 Bio-Rad Laboratories, Inc. Enzyme quantification
JP5188314B2 (ja) 2008-08-04 2013-04-24 キヤノン株式会社 生体高分子検査装置及びその方法
WO2010033200A2 (en) * 2008-09-19 2010-03-25 President And Fellows Of Harvard College Creation of libraries of droplets and related species
US8748094B2 (en) 2008-12-19 2014-06-10 President And Fellows Of Harvard College Particle-assisted nucleic acid sequencing
WO2010093907A2 (en) * 2009-02-13 2010-08-19 The Board Of Regents Of The University Of Texas System An assay for the detection of recurrence in breast cancer using the novel tumor suppressor dear1
US9303287B2 (en) 2009-02-26 2016-04-05 Dako Denmark A/S Compositions and methods for RNA hybridization applications
EP3663750B1 (de) 2009-05-29 2021-11-03 Life Technologies Corporation Nukleinsäuregerüstpolymerpartikel und verfahren zur herstellung und verwendung
CN102648053B (zh) 2009-10-27 2016-04-27 哈佛学院院长等 液滴生成技术
US8535889B2 (en) 2010-02-12 2013-09-17 Raindance Technologies, Inc. Digital analyte analysis
US9399797B2 (en) 2010-02-12 2016-07-26 Raindance Technologies, Inc. Digital analyte analysis
US9562897B2 (en) 2010-09-30 2017-02-07 Raindance Technologies, Inc. Sandwich assays in droplets
WO2012079749A1 (en) * 2010-12-16 2012-06-21 Stichting Dutch Polymer Institute Process for making cell suspension
EP3412778A1 (de) 2011-02-11 2018-12-12 Raindance Technologies, Inc. Verfahren zur bildung gemischter tröpfchen
EP3736281A1 (de) 2011-02-18 2020-11-11 Bio-Rad Laboratories, Inc. Zusammensetzungen und verfahren für molekulare etikettierung
EP3709018A1 (de) 2011-06-02 2020-09-16 Bio-Rad Laboratories, Inc. Mikrofluidische vorrichtung zum nachweis von komponenten einer chemischen reaktion
EP2729501A2 (de) 2011-07-07 2014-05-14 Life Technologies Corporation Polymerteilchen, nukleinsäurepolymerpartikel sowie verfahren zu ihrer herstellung und verwendung
US8658430B2 (en) 2011-07-20 2014-02-25 Raindance Technologies, Inc. Manipulating droplet size
US10662465B2 (en) * 2011-09-30 2020-05-26 Agilent Technologies, Inc. Hybridization compositions and methods using formamide
US10501791B2 (en) 2011-10-14 2019-12-10 President And Fellows Of Harvard College Sequencing by structure assembly
EP3252173A1 (de) 2011-10-21 2017-12-06 Dako Denmark A/S Hybridisierungszusammensetzungen und -verfahren
EP4249605B1 (de) 2011-12-22 2024-08-28 President And Fellows Of Harvard College Verfahren zum nachweis von analyten
US11021737B2 (en) 2011-12-22 2021-06-01 President And Fellows Of Harvard College Compositions and methods for analyte detection
WO2014163886A1 (en) 2013-03-12 2014-10-09 President And Fellows Of Harvard College Method of generating a three-dimensional nucleic acid containing matrix
WO2013101741A1 (en) 2011-12-30 2013-07-04 Abbott Molecular, Inc. Channels with cross-sectional thermal gradients
US20150177115A1 (en) 2012-04-06 2015-06-25 Slingshot Biosciences Hydrogel particles with tunable optical properties
MX357318B (es) * 2012-04-27 2018-07-04 Cepheid Aparato con modulos de procesamiento heterogeneos.
WO2013184754A2 (en) 2012-06-05 2013-12-12 President And Fellows Of Harvard College Spatial sequencing of nucleic acids using dna origami probes
US10273541B2 (en) 2012-08-14 2019-04-30 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10221442B2 (en) 2012-08-14 2019-03-05 10X Genomics, Inc. Compositions and methods for sample processing
US10323279B2 (en) 2012-08-14 2019-06-18 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10752949B2 (en) 2012-08-14 2020-08-25 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10584381B2 (en) 2012-08-14 2020-03-10 10X Genomics, Inc. Methods and systems for processing polynucleotides
CN104769127A (zh) * 2012-08-14 2015-07-08 10X基因组学有限公司 微胶囊组合物及方法
US11591637B2 (en) 2012-08-14 2023-02-28 10X Genomics, Inc. Compositions and methods for sample processing
US9701998B2 (en) 2012-12-14 2017-07-11 10X Genomics, Inc. Methods and systems for processing polynucleotides
US9951386B2 (en) 2014-06-26 2018-04-24 10X Genomics, Inc. Methods and systems for processing polynucleotides
CA3212874A1 (en) * 2012-09-26 2014-04-03 Quantumcyte, Inc. Devices and methods for single cell analysis
EP3567116A1 (de) 2012-12-14 2019-11-13 10X Genomics, Inc. Verfahren und systeme zur verarbeitung von polynukleotiden
US10533221B2 (en) 2012-12-14 2020-01-14 10X Genomics, Inc. Methods and systems for processing polynucleotides
KR20200140929A (ko) * 2013-02-08 2020-12-16 10엑스 제노믹스, 인크. 폴리뉴클레오티드 바코드 생성
US10395758B2 (en) 2013-08-30 2019-08-27 10X Genomics, Inc. Sequencing methods
CN105658817A (zh) 2013-09-13 2016-06-08 生命技术公司 使用凝聚核酸颗粒的装置制备
US11901041B2 (en) 2013-10-04 2024-02-13 Bio-Rad Laboratories, Inc. Digital analysis of nucleic acid modification
WO2015069798A1 (en) * 2013-11-05 2015-05-14 The Regents Of The University Of California Single-cell forensic short tandem repeat typing within microfluidic droplets
WO2015069634A1 (en) 2013-11-08 2015-05-14 President And Fellows Of Harvard College Microparticles, methods for their preparation and use
US9944977B2 (en) 2013-12-12 2018-04-17 Raindance Technologies, Inc. Distinguishing rare variations in a nucleic acid sequence from a sample
US9824068B2 (en) 2013-12-16 2017-11-21 10X Genomics, Inc. Methods and apparatus for sorting data
CN110548550B (zh) 2014-04-10 2022-03-08 10X基因组学有限公司 用于封装和分割试剂的流体装置、系统和方法及其应用
CN110211637B (zh) 2014-06-26 2023-10-27 10X基因组学有限公司 核酸序列装配的方法和系统
CA2953374A1 (en) 2014-06-26 2015-12-30 10X Genomics, Inc. Methods of analyzing nucleic acids from individual cells or cell populations
WO2016049936A1 (en) * 2014-09-30 2016-04-07 Bgi Shenzhen Co., Limited Biomarkers for rheumatoid arthritis and usage therof
KR20170073667A (ko) 2014-10-29 2017-06-28 10엑스 제노믹스, 인크. 표적화 핵산 서열 분석을 위한 방법 및 조성물
US9975122B2 (en) 2014-11-05 2018-05-22 10X Genomics, Inc. Instrument systems for integrated sample processing
US10221436B2 (en) 2015-01-12 2019-03-05 10X Genomics, Inc. Processes and systems for preparation of nucleic acid sequencing libraries and libraries prepared using same
CN107209814B (zh) 2015-01-13 2021-10-15 10X基因组学有限公司 用于使结构变异和相位信息可视化的系统和方法
EP3256606B1 (de) 2015-02-09 2019-05-22 10X Genomics, Inc. Systeme und verfahren zur bestimmung struktureller varianten
EP3256850A4 (de) 2015-02-09 2018-10-10 Slingshot Biosciences, Inc. Hydrogelpartikel mit abstimmbaren optischen eigenschaften und verfahren zur verwendung davon
WO2016137973A1 (en) 2015-02-24 2016-09-01 10X Genomics Inc Partition processing methods and systems
US11274343B2 (en) 2015-02-24 2022-03-15 10X Genomics, Inc. Methods and compositions for targeted nucleic acid sequence coverage
US10059990B2 (en) 2015-04-14 2018-08-28 Massachusetts Institute Of Technology In situ nucleic acid sequencing of expanded biological samples
US10526649B2 (en) 2015-04-14 2020-01-07 Massachusetts Institute Of Technology Augmenting in situ nucleic acid sequencing of expanded biological samples with in vitro sequence information
US11408890B2 (en) 2015-04-14 2022-08-09 Massachusetts Institute Of Technology Iterative expansion microscopy
WO2017027368A1 (en) 2015-08-07 2017-02-16 Massachusetts Institute Of Technology Protein retention expansion microscopy
CA2994958C (en) * 2015-08-07 2024-02-13 Massachusetts Institute Of Technology Nanoscale imaging of proteins and nucleic acids via expansion microscopy
JP2018537414A (ja) 2015-10-13 2018-12-20 プレジデント アンド フェローズ オブ ハーバード カレッジ ゲルマイクロスフェアの作製及び使用のためのシステム及び方法
JP6882282B2 (ja) 2015-11-03 2021-06-02 プレジデント アンド フェローズ オブ ハーバード カレッジ 三次元核酸含有マトリックスの立体撮像のための方法と装置
US11371094B2 (en) 2015-11-19 2022-06-28 10X Genomics, Inc. Systems and methods for nucleic acid processing using degenerate nucleotides
PT3882357T (pt) 2015-12-04 2022-09-05 10X Genomics Inc Métodos e composições para análise de ácidos nucleicos
SG11201806757XA (en) 2016-02-11 2018-09-27 10X Genomics Inc Systems, methods, and media for de novo assembly of whole genome sequence data
WO2017189525A1 (en) 2016-04-25 2017-11-02 President And Fellows Of Harvard College Hybridization chain reaction methods for in situ molecular detection
WO2017197338A1 (en) 2016-05-13 2017-11-16 10X Genomics, Inc. Microfluidic systems and methods of use
WO2018045181A1 (en) 2016-08-31 2018-03-08 President And Fellows Of Harvard College Methods of generating libraries of nucleic acid sequences for detection via fluorescent in situ sequencing
WO2018045186A1 (en) 2016-08-31 2018-03-08 President And Fellows Of Harvard College Methods of combining the detection of biomolecules into a single assay using fluorescent in situ sequencing
AU2017326338A1 (en) 2016-09-15 2019-01-31 Augmenta Bioworks, Inc. Immune repertoire sequence amplification methods and applications
US10815525B2 (en) 2016-12-22 2020-10-27 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10011872B1 (en) 2016-12-22 2018-07-03 10X Genomics, Inc. Methods and systems for processing polynucleotides
US10550429B2 (en) 2016-12-22 2020-02-04 10X Genomics, Inc. Methods and systems for processing polynucleotides
WO2018136856A1 (en) 2017-01-23 2018-07-26 Massachusetts Institute Of Technology Multiplexed signal amplified fish via splinted ligation amplification and sequencing
CN117512066A (zh) 2017-01-30 2024-02-06 10X基因组学有限公司 用于基于微滴的单细胞条形编码的方法和系统
US10995333B2 (en) 2017-02-06 2021-05-04 10X Genomics, Inc. Systems and methods for nucleic acid preparation
WO2018157048A1 (en) 2017-02-24 2018-08-30 Massachusetts Institute Of Technology Methods for examining podocyte foot processes in human renal samples using conventional optical microscopy
WO2018157074A1 (en) 2017-02-24 2018-08-30 Massachusetts Institute Of Technology Methods for diagnosing neoplastic lesions
CN106868148B (zh) * 2017-03-08 2021-02-26 中国科学院苏州生物医学工程技术研究所 聚集状态可控且能够长期保存的细胞核的制备方法
WO2018213774A1 (en) 2017-05-19 2018-11-22 10X Genomics, Inc. Systems and methods for analyzing datasets
US10400235B2 (en) 2017-05-26 2019-09-03 10X Genomics, Inc. Single cell analysis of transposase accessible chromatin
CN116064732A (zh) 2017-05-26 2023-05-05 10X基因组学有限公司 转座酶可接近性染色质的单细胞分析
WO2019023214A1 (en) 2017-07-25 2019-01-31 Massachusetts Institute Of Technology ATAC IN SITU SEQUENCING
US10590244B2 (en) 2017-10-04 2020-03-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
US10837047B2 (en) 2017-10-04 2020-11-17 10X Genomics, Inc. Compositions, methods, and systems for bead formation using improved polymers
WO2019084043A1 (en) 2017-10-26 2019-05-02 10X Genomics, Inc. METHODS AND SYSTEMS FOR NUCLEIC ACID PREPARATION AND CHROMATIN ANALYSIS
EP3700672B1 (de) 2017-10-27 2022-12-28 10X Genomics, Inc. Verfahren zur probenvorbereitung und -analyse
SG11201913654QA (en) 2017-11-15 2020-01-30 10X Genomics Inc Functionalized gel beads
US10829815B2 (en) 2017-11-17 2020-11-10 10X Genomics, Inc. Methods and systems for associating physical and genetic properties of biological particles
WO2019108851A1 (en) 2017-11-30 2019-06-06 10X Genomics, Inc. Systems and methods for nucleic acid preparation and analysis
WO2019126789A1 (en) 2017-12-22 2019-06-27 10X Genomics, Inc. Systems and methods for processing nucleic acid molecules from one or more cells
US11873374B2 (en) 2018-02-06 2024-01-16 Massachusetts Institute Of Technology Swellable and structurally homogenous hydrogels and methods of use thereof
WO2019157529A1 (en) 2018-02-12 2019-08-15 10X Genomics, Inc. Methods characterizing multiple analytes from individual cells or cell populations
US11639928B2 (en) 2018-02-22 2023-05-02 10X Genomics, Inc. Methods and systems for characterizing analytes from individual cells or cell populations
WO2019169028A1 (en) 2018-02-28 2019-09-06 10X Genomics, Inc. Transcriptome sequencing through random ligation
EP3775271A1 (de) 2018-04-06 2021-02-17 10X Genomics, Inc. Systeme und verfahren zur qualitätskontrolle in einer einzelzellenverarbeitung
WO2019217758A1 (en) 2018-05-10 2019-11-14 10X Genomics, Inc. Methods and systems for molecular library generation
US11932899B2 (en) 2018-06-07 2024-03-19 10X Genomics, Inc. Methods and systems for characterizing nucleic acid molecules
US11703427B2 (en) 2018-06-25 2023-07-18 10X Genomics, Inc. Methods and systems for cell and bead processing
US20200032335A1 (en) 2018-07-27 2020-01-30 10X Genomics, Inc. Systems and methods for metabolome analysis
CN112770776A (zh) 2018-07-30 2021-05-07 瑞德库尔有限责任公司 用于样品处理或分析的方法和系统
US20200048626A1 (en) * 2018-08-07 2020-02-13 Augmenta Bioworks, Inc. Methods for Making Gel Beads and Core and Shell Beads with a Cell
US12065688B2 (en) 2018-08-20 2024-08-20 10X Genomics, Inc. Compositions and methods for cellular processing
WO2020076976A1 (en) 2018-10-10 2020-04-16 Readcoor, Inc. Three-dimensional spatial molecular indexing
US11662341B2 (en) 2018-10-10 2023-05-30 Augmenta Bioworks, Inc. Methods for isolating immune binding proteins
US11459607B1 (en) 2018-12-10 2022-10-04 10X Genomics, Inc. Systems and methods for processing-nucleic acid molecules from a single cell using sequential co-partitioning and composite barcodes
US11845983B1 (en) 2019-01-09 2023-12-19 10X Genomics, Inc. Methods and systems for multiplexing of droplet based assays
US11467153B2 (en) 2019-02-12 2022-10-11 10X Genomics, Inc. Methods for processing nucleic acid molecules
US11851683B1 (en) 2019-02-12 2023-12-26 10X Genomics, Inc. Methods and systems for selective analysis of cellular samples
EP3924505A1 (de) 2019-02-12 2021-12-22 10X Genomics, Inc. Verfahren zur verarbeitung von nukleinsäuremolekülen
US11655499B1 (en) 2019-02-25 2023-05-23 10X Genomics, Inc. Detection of sequence elements in nucleic acid molecules
SG11202111242PA (en) 2019-03-11 2021-11-29 10X Genomics Inc Systems and methods for processing optically tagged beads
WO2020218551A1 (ja) * 2019-04-26 2020-10-29 bitBiome株式会社 ゲルカプセル方式による1細胞ゲノムライブラリーからの配列スクリーニング法
DE102019208833A1 (de) * 2019-06-18 2020-12-24 Robert Bosch Gmbh Verfahren zum Kennzeichnen von Pflanzenbildinformationen, insbesondere für landwirtschaftliche Zwecke
WO2021113505A1 (en) 2019-12-05 2021-06-10 Massachusetts Institute Of Technology Method for preparing a specimen for expansion microscopy
CA3165821A1 (en) 2020-01-24 2021-07-29 Jeffrey Kim Compositions and methods for cell-like calibration particles
WO2021226036A1 (en) 2020-05-04 2021-11-11 Slingshot Biosciences, Inc. Compositions and methods for passive optical barcoding for multiplexed assays
US11851700B1 (en) 2020-05-13 2023-12-26 10X Genomics, Inc. Methods, kits, and compositions for processing extracellular molecules
US12084715B1 (en) 2020-11-05 2024-09-10 10X Genomics, Inc. Methods and systems for reducing artifactual antisense products
EP4298244A1 (de) 2021-02-23 2024-01-03 10X Genomics, Inc. Sonden-basierte analyse von nukleinsäuren und proteinen
CN113293192A (zh) * 2021-05-31 2021-08-24 苏州市立医院(北区) 中性粒细胞趋化检测试剂盒
KR20240116721A (ko) 2021-10-29 2024-07-30 슬링샷 바이오사이언시즈 인코포레이티드 피더 세포 및 합성 항원 제시 세포로서의 하이드로겔 입자
WO2023235885A1 (en) 2022-06-02 2023-12-07 Slingshot Biosciences, Inc. Apoptotic cell mimic

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4695548A (en) * 1982-11-18 1987-09-22 The Trustees Of Columbia University In The City Of New York Gel inserts useful in electrophoresis
US4996144A (en) * 1985-01-25 1991-02-26 Calgene, Inc. Microassay for detection of DNA and RNA
SE458525B (sv) * 1985-05-23 1989-04-10 Pharmacia Ab Foerfarande foer tvaerbindning av en poroes agar- eller agarosgel
US5225332A (en) * 1988-04-22 1993-07-06 Massachusetts Institute Of Technology Process for manipulation of non-aqueous surrounded microdroplets
US5188963A (en) 1989-11-17 1993-02-23 Gene Tec Corporation Device for processing biological specimens for analysis of nucleic acids
US5665582A (en) 1990-10-29 1997-09-09 Dekalb Genetics Corp. Isolation of biological materials
JPH06510363A (ja) * 1990-10-29 1994-11-17 ディカルブ プラント ジェネティクス 磁気性粒子を使用する生物学的材料の単離
US5784162A (en) 1993-08-18 1998-07-21 Applied Spectral Imaging Ltd. Spectral bio-imaging methods for biological research, medical diagnostics and therapy
RU1794088C (ru) 1991-03-18 1993-02-07 Институт Молекулярной Биологии Ан@ Ссср Способ определени нуклеотидной последовательности ДНК и устройство дл его осуществлени
AU662906B2 (en) * 1991-06-26 1995-09-21 F. Hoffmann-La Roche Ag Methods for detection of carcinoma metastases by nucleic acid amplification
EP0626455B1 (de) * 1993-05-28 1998-07-15 Enea Ente Per Le Nuove Tecnologie, L'energia E L'ambiente Verfahren zur Analyse, Abtrennung und Mikroverkapselung von zellulären Elementen
US5576176A (en) * 1994-03-03 1996-11-19 The Regents Of The University Of California Marker and an assay for detection and monitoring of human immunodeficiency virus latency and activation
US5861247A (en) 1996-01-26 1999-01-19 University Of Chicago Rapid method to detect duplex formation in sequencing by hybridization methods
JP2002531056A (ja) * 1998-08-07 2002-09-24 セレイ, エルエルシー 遺伝子分析におけるゲルマイクロドロップ

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US20070020617A1 (en) 2007-01-25
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CA2339734A1 (en) 2000-02-17
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