AR127146A1 - COMPOSITIONS AND METHODS FOR THE TRANSFORMATION OF EXPLANTS OF EMBRYOS REMOVED FROM MONOCOTYLEDON SEEDS - Google Patents
COMPOSITIONS AND METHODS FOR THE TRANSFORMATION OF EXPLANTS OF EMBRYOS REMOVED FROM MONOCOTYLEDON SEEDSInfo
- Publication number
- AR127146A1 AR127146A1 ARP220102579A ARP220102579A AR127146A1 AR 127146 A1 AR127146 A1 AR 127146A1 AR P220102579 A ARP220102579 A AR P220102579A AR P220102579 A ARP220102579 A AR P220102579A AR 127146 A1 AR127146 A1 AR 127146A1
- Authority
- AR
- Argentina
- Prior art keywords
- plant
- contact
- genetically modified
- explant
- methods
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 241000209510 Liliopsida Species 0.000 title abstract 3
- 239000000203 mixture Substances 0.000 title abstract 2
- 210000002257 embryonic structure Anatomy 0.000 title 1
- 230000009466 transformation Effects 0.000 title 1
- 241000196324 Embryophyta Species 0.000 abstract 9
- 210000001161 mammalian embryo Anatomy 0.000 abstract 5
- 230000001172 regenerating effect Effects 0.000 abstract 3
- 230000008929 regeneration Effects 0.000 abstract 3
- 238000011069 regeneration method Methods 0.000 abstract 3
- 229930192334 Auxin Natural products 0.000 abstract 2
- 239000002363 auxin Substances 0.000 abstract 2
- UQHKFADEQIVWID-UHFFFAOYSA-N cytokinin Natural products C1=NC=2C(NCC=C(CO)C)=NC=NC=2N1C1CC(O)C(CO)O1 UQHKFADEQIVWID-UHFFFAOYSA-N 0.000 abstract 2
- 239000004062 cytokinin Substances 0.000 abstract 2
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 abstract 2
- 230000006698 induction Effects 0.000 abstract 2
- 102000040430 polynucleotide Human genes 0.000 abstract 2
- 108091033319 polynucleotide Proteins 0.000 abstract 2
- 239000002157 polynucleotide Substances 0.000 abstract 2
- 150000003839 salts Chemical class 0.000 abstract 2
- 238000012258 culturing Methods 0.000 abstract 1
- 230000000442 meristematic effect Effects 0.000 abstract 1
- 230000001131 transforming effect Effects 0.000 abstract 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/008—Methods for regeneration to complete plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Developmental Biology & Embryology (AREA)
- Genetics & Genomics (AREA)
- Cell Biology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Botany (AREA)
- Environmental Sciences (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Microbiology (AREA)
- Plant Pathology (AREA)
- Biophysics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
La invención proporciona composiciones y métodos novedosos para regenerar una planta monocotiledónea o parte de la planta genéticamente modificada en contacto con un medio de regeneración que tiene una baja concentración de sal. Los métodos proporcionados en el presente documento pueden incluir la transformación de al menos una célula vegetal del explante de embrión con un polinucleótido heterólogo, cultivar el explante de embrión en contacto con un primer medio de inducción de brotes que una auxina y una citoquinina y regenerar una planta o parte de la planta genéticamente modificada a partir de una célula vegetal o explante transformado o editado. Reivindicación 1: Un método para producir una planta monocotiledónea o parte de la planta genéticamente modificada que comprende: introducir una molécula polinucleotídica heteróloga en al menos una célula de un explante de embrión de semilla monocotiledónea que comprende tejido meristemático cultivar el explante de embrión en contacto con un primer medio de inducción de brotes que comprende una primera auxina y una primera citoquinina, y regenerar la planta monocotiledónea o parte de la planta genéticamente modificada a partir del explante de embrión cultivado en contacto con un medio de regeneración, en donde el medio de regeneración tiene una baja concentración de sal.The invention provides novel compositions and methods for regenerating a genetically modified monocotyledonous plant or plant part in contact with a regeneration medium having a low salt concentration. The methods provided herein may include transforming at least one plant cell of the embryo explant with a heterologous polynucleotide, culturing the embryo explant in contact with a first sprout induction medium containing an auxin and a cytokinin, and regenerating a genetically modified plant or part of a plant from a transformed or edited plant cell or explant. Claim 1: A method of producing a genetically modified monocot plant or plant part comprising: introducing a heterologous polynucleotide molecule into at least one cell of a monocot seed embryo explant comprising meristematic tissue cultivating the embryo explant in contact with a first shoot induction medium comprising a first auxin and a first cytokinin, and regenerating the monocotyledonous plant or part of the genetically modified plant from the embryo explant cultured in contact with a regeneration medium, wherein the regeneration medium It has a low concentration of salt.
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US202163248960P | 2021-09-27 | 2021-09-27 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| AR127146A1 true AR127146A1 (en) | 2023-12-20 |
Family
ID=85721327
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| ARP220102579A AR127146A1 (en) | 2021-09-27 | 2022-09-26 | COMPOSITIONS AND METHODS FOR THE TRANSFORMATION OF EXPLANTS OF EMBRYOS REMOVED FROM MONOCOTYLEDON SEEDS |
Country Status (2)
| Country | Link |
|---|---|
| AR (1) | AR127146A1 (en) |
| WO (1) | WO2023049903A1 (en) |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CA2505499C (en) * | 2001-11-13 | 2010-02-02 | Agricore United | Methods of plant regeneration and transformation |
| US7354760B2 (en) * | 2001-12-26 | 2008-04-08 | University Of Central Florida Research Foundation, Inc. | Expression of protective antigens in transgenic chloroplasts |
| US8581035B2 (en) * | 2006-08-31 | 2013-11-12 | Monsanto Technology Llc | Plant transformation without selection |
| US11807846B2 (en) * | 2012-04-29 | 2023-11-07 | Monsanto Technology Llc | Methods for transforming corn explants |
| WO2019227030A1 (en) * | 2018-05-24 | 2019-11-28 | Monsanto Technology Llc | Genome editing in plants |
-
2022
- 2022-09-26 WO PCT/US2022/077031 patent/WO2023049903A1/en active Application Filing
- 2022-09-26 AR ARP220102579A patent/AR127146A1/en unknown
Also Published As
| Publication number | Publication date |
|---|---|
| WO2023049903A8 (en) | 2023-12-14 |
| WO2023049903A1 (en) | 2023-03-30 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| FB | Suspension of granting procedure |