AR126938A1 - Métodos y composiciones para mejorar la arquitectura de las plantas y los rasgos de rendimiento - Google Patents
Métodos y composiciones para mejorar la arquitectura de las plantas y los rasgos de rendimientoInfo
- Publication number
- AR126938A1 AR126938A1 ARP220102358A ARP220102358A AR126938A1 AR 126938 A1 AR126938 A1 AR 126938A1 AR P220102358 A ARP220102358 A AR P220102358A AR P220102358 A ARP220102358 A AR P220102358A AR 126938 A1 AR126938 A1 AR 126938A1
- Authority
- AR
- Argentina
- Prior art keywords
- gene
- max1
- plant
- glyma
- endogenous
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 3
- 239000000203 mixture Substances 0.000 title abstract 3
- 108090000623 proteins and genes Proteins 0.000 abstract 18
- 241000196324 Embryophyta Species 0.000 abstract 15
- 230000035772 mutation Effects 0.000 abstract 5
- 102000039446 nucleic acids Human genes 0.000 abstract 5
- 108020004707 nucleic acids Proteins 0.000 abstract 5
- 150000007523 nucleic acids Chemical class 0.000 abstract 5
- 108091007494 Nucleic acid- binding domains Proteins 0.000 abstract 2
- 102000002004 Cytochrome P-450 Enzyme System Human genes 0.000 abstract 1
- 244000068988 Glycine max Species 0.000 abstract 1
- 101710198130 NADPH-cytochrome P450 reductase Proteins 0.000 abstract 1
- 101710163270 Nuclease Proteins 0.000 abstract 1
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 1
- 230000037429 base substitution Effects 0.000 abstract 1
- 230000037430 deletion Effects 0.000 abstract 1
- 238000012217 deletion Methods 0.000 abstract 1
- 239000012636 effector Substances 0.000 abstract 1
- 238000010362 genome editing Methods 0.000 abstract 1
- 230000037431 insertion Effects 0.000 abstract 1
- 238000003780 insertion Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
- 239000002773 nucleotide Substances 0.000 abstract 1
- 125000003729 nucleotide group Chemical group 0.000 abstract 1
- 229920001184 polypeptide Polymers 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 102000004169 proteins and genes Human genes 0.000 abstract 1
- 125000006850 spacer group Chemical group 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H1/00—Processes for modifying genotypes ; Plants characterised by associated natural traits
- A01H1/06—Processes for producing mutations, e.g. treatment with chemicals or with radiation
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H5/00—Angiosperms, i.e. flowering plants, characterised by their plant parts; Angiosperms characterised otherwise than by their botanic taxonomy
- A01H5/10—Seeds
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H6/00—Angiosperms, i.e. flowering plants, characterised by their botanic taxonomy
- A01H6/54—Leguminosae or Fabaceae, e.g. soybean, alfalfa or peanut
- A01H6/542—Glycine max [soybean]
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases RNAses, DNAses
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Botany (AREA)
- Microbiology (AREA)
- Environmental Sciences (AREA)
- Medicinal Chemistry (AREA)
- Developmental Biology & Embryology (AREA)
- Physiology (AREA)
- Biophysics (AREA)
- Nutrition Science (AREA)
- Plant Pathology (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Esta invención se refiere a composiciones y métodos para modificar genes de Más Crecimiento Axilar 1 (MAX1) en plantas, opcionalmente, para mejorar la arquitectura de la planta y/o para mejorados rasgos de rendimiento. La invención además se refiere a plantas que tienen mejorada arquitectura de la planta y/o rasgos de rendimiento mejorado producidas usando los métodos y las composiciones de la invención. Reivindicación 1: Una planta o parte de planta de la misma que comprende por lo menos una mutación en un gen endógeno de Más Crecimiento Axilar 1 (MAX1) que codifica un polipéptido de monooxigenasa de citocromo P450 (MAX1). Reivindicación 35: Una célula vegetal que comprende por lo menos una mutación dentro de un gen endógeno de Más Crecimiento Axilar 1 (MAX1), donde la por lo menos una mutación es una sustitución de base, inserción de base, o deleción de base que se introduce usando un sistema de edición que comprende un dominio de unión de ácido nucleico que se une a un sitio diana en el gen endógeno MAX1. Reivindicación 63: Un método para elaborar una planta, que comprende: (a) poner en contacto una población de células vegetales que comprenden un gen endógeno de Más Crecimiento Axilar 1 (MAX1) con una nucleasa unida a un dominio de unión de ácido nucleico (por ej., sistema de edición) que se une a una secuencia (i) que tiene por lo menos 80% de identidad de secuencia con una secuencia de nucleótidos de cualquiera de SEQ ID Nº 69, 70, 93, 94, 115, 116, 140 o 141, (ii) que comprende una región que tiene por lo menos 90% de identidad con cualquiera de SEQ ID Nº 72 - 91, 96 - 113, 118 - 138, o 143 - 164; (iii) que codifica una secuencia de aminoácidos que tiene por lo menos 80% de identidad de secuencia con cualquiera de SEQ ID Nº 71, 95, 117, o 142, y/o (iv) que codifica una región que tiene por lo menos 90% de identidad de secuencia con cualquiera de SEQ ID Nº 92, 114, 139 o 165, y/o; (b) seleccionar una célula vegetal de la población de células vegetales en la cual un gen endógeno MAX1 ha sido mutado, a fin de producir una célula vegetal que comprende una mutación en el gen endógeno MAX1; y (c) cultivar la célula vegetal seleccionada hasta lograr una planta. Reivindicación 80: Un ácido nucleico guía que se une a un sitio diana en un gen de Más Crecimiento Axilar 1 (MAX1), donde el sitio diana está en una región del gen MAX1 que tiene por lo menos 90% de identidad de secuencia con cualquiera de SEQ ID Nº 72 - 91, 96 - 113, 118 - 138, o 143 - 164, opcionalmente una región del gen MAX1 que tiene por lo menos 90% de identidad de secuencia con cualquiera de SEQ ID Nº 77 - 79, 81 - 83, 88, 90, 91, 101 - 103, 105 - 107, 113, 121, 124, 125, 127 - 129, 132 - 138, 148 - 150, 152 - 154, o 160 - 164. Reivindicación 84: Un sistema de edición de genes que comprende una proteína efectora CRISPR-CAS en asociación con un ácido nucleico guía, donde el ácido nucleico guía comprende una secuencia espaciadora que se une a un gen endógeno de Más Crecimiento Axilar 1 (MAX1). Reivindicación 91: Una planta de soja o parte de planta de ella que comprende por lo menos una mutación no natural en por lo menos un gen endógeno de Más Crecimiento Axilar 1 (MAX1) que tiene el número de identificación de gen (gen ID) de Glyma.04g052100 (MAX1a), Glyma.06g052700 (MAX1b), Glyma.14g096900 (MAX1c) y/o Glyma.17g227500 (MAX1d). Reivindicación 92: Un ácido nucleico guía que se une a un ácido nucleico diana en un gen de Más Crecimiento Axilar 1 (MAX1) que tiene el número de identificación de gen (gen ID) de Glyma.04g052100 (MAX1a), Glyma.06g052700 (MAX1b), Glyma.14g096900 (MAX1c) y/o Glyma.17g227500 (MAX1d).
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US202163240132P | 2021-09-02 | 2021-09-02 |
Publications (1)
Publication Number | Publication Date |
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AR126938A1 true AR126938A1 (es) | 2023-11-29 |
Family
ID=83439134
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP220102358A AR126938A1 (es) | 2021-09-02 | 2022-08-31 | Métodos y composiciones para mejorar la arquitectura de las plantas y los rasgos de rendimiento |
Country Status (7)
Country | Link |
---|---|
US (1) | US20230151379A1 (es) |
EP (1) | EP4396210A1 (es) |
CN (1) | CN118176205A (es) |
AR (1) | AR126938A1 (es) |
CA (1) | CA3230668A1 (es) |
MX (1) | MX2024002417A (es) |
WO (1) | WO2023034891A1 (es) |
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-
2022
- 2022-08-31 AR ARP220102358A patent/AR126938A1/es unknown
- 2022-09-01 WO PCT/US2022/075806 patent/WO2023034891A1/en active Application Filing
- 2022-09-01 US US17/929,061 patent/US20230151379A1/en active Pending
- 2022-09-01 CN CN202280070281.0A patent/CN118176205A/zh active Pending
- 2022-09-01 CA CA3230668A patent/CA3230668A1/en active Pending
- 2022-09-01 MX MX2024002417A patent/MX2024002417A/es unknown
- 2022-09-01 EP EP22777165.6A patent/EP4396210A1/en active Pending
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CN118176205A (zh) | 2024-06-11 |
CA3230668A1 (en) | 2023-03-09 |
EP4396210A1 (en) | 2024-07-10 |
WO2023034891A1 (en) | 2023-03-09 |
US20230151379A1 (en) | 2023-05-18 |
MX2024002417A (es) | 2024-04-05 |
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