AR069653A2 - Variante de glucoamilasa original de aspergillus, una construccion de adn, un vector de expresion recombinante, una celula, un proceso para convertir almidon, uso de una variante de glucoamilasa para un proceso de la fermentacion y metodo para alterar la estabilidad termica. - Google Patents
Variante de glucoamilasa original de aspergillus, una construccion de adn, un vector de expresion recombinante, una celula, un proceso para convertir almidon, uso de una variante de glucoamilasa para un proceso de la fermentacion y metodo para alterar la estabilidad termica.Info
- Publication number
- AR069653A2 AR069653A2 ARP080105383A ARP080105383A AR069653A2 AR 069653 A2 AR069653 A2 AR 069653A2 AR P080105383 A ARP080105383 A AR P080105383A AR P080105383 A ARP080105383 A AR P080105383A AR 069653 A2 AR069653 A2 AR 069653A2
- Authority
- AR
- Argentina
- Prior art keywords
- glucoamylase
- variante
- amino acid
- original
- almidon
- Prior art date
Links
- 108010073178 Glucan 1,4-alpha-Glucosidase Proteins 0.000 title abstract 5
- 102100022624 Glucoamylase Human genes 0.000 title abstract 5
- 238000000034 method Methods 0.000 title 3
- 241000228212 Aspergillus Species 0.000 title 1
- 238000010276 construction Methods 0.000 title 1
- 239000013604 expression vector Substances 0.000 title 1
- 238000000855 fermentation Methods 0.000 title 1
- 230000004151 fermentation Effects 0.000 title 1
- 238000003259 recombinant expression Methods 0.000 title 1
- 150000001413 amino acids Chemical class 0.000 abstract 5
- 125000003275 alpha amino acid group Chemical group 0.000 abstract 3
- 230000004075 alteration Effects 0.000 abstract 2
- 230000037431 insertion Effects 0.000 abstract 1
- 238000003780 insertion Methods 0.000 abstract 1
- 238000006467 substitution reaction Methods 0.000 abstract 1
- 230000001629 suppression Effects 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
- C12N9/2411—Amylases
- C12N9/2428—Glucan 1,4-alpha-glucosidase (3.2.1.3), i.e. glucoamylase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/20—Preparation of compounds containing saccharide radicals produced by the action of an exo-1,4 alpha-glucosidase, e.g. dextrose
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Microbiology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Medicinal Chemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
Una variante de una glucoamilasa original, que comprende una alteracion en una o más de las siguientes posiciones: 59, 66, 72, 119, 189, 223, 227, 313, 340, 342, 352, 379, 386, 393, 395, 402, 408, 416, 425, 427, 444, 486, 490, 494. En la cual (a) la alteracion es independientemente (iv) una insercion de un aminoácido corriente abajo aminoácido del aminoácido que ocupa la posicion, (v) una supresion del aminoácido que ocupa la posicion, o (vi) una substitucion del aminoácido que ocupa la posicion con un aminoácido diferente, (b) la variante tiene actividad glucoamilasa y (c) cada posicion corresponde a la posicion de la secuencia de aminoácidos de la glucoamilasa original que tiene la secuencia de aminoácidos de SEQ. ID N°: 2, que exhibe propiedades alteradas, en particular en su estabilidad térmica y actividad específica.
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DKPA199900999 | 1999-07-09 |
Publications (1)
Publication Number | Publication Date |
---|---|
AR069653A2 true AR069653A2 (es) | 2010-02-10 |
Family
ID=8099879
Family Applications (2)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP000103471A AR029759A1 (es) | 1999-07-09 | 2000-07-07 | Variante de glucoamilasa original, una construccion de adn, un vector de expresion recombinante, una celula, un proceso para convertir almidon, uso de una variante de glucoamilasa para un proceso de fermentacion y metodo para mejorar la estabilidad termica. |
ARP080105383A AR069653A2 (es) | 1999-07-09 | 2008-12-11 | Variante de glucoamilasa original de aspergillus, una construccion de adn, un vector de expresion recombinante, una celula, un proceso para convertir almidon, uso de una variante de glucoamilasa para un proceso de la fermentacion y metodo para alterar la estabilidad termica. |
Family Applications Before (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
ARP000103471A AR029759A1 (es) | 1999-07-09 | 2000-07-07 | Variante de glucoamilasa original, una construccion de adn, un vector de expresion recombinante, una celula, un proceso para convertir almidon, uso de una variante de glucoamilasa para un proceso de fermentacion y metodo para mejorar la estabilidad termica. |
Country Status (8)
Country | Link |
---|---|
US (3) | US7354753B2 (es) |
EP (2) | EP1200566A2 (es) |
JP (1) | JP2003504046A (es) |
CN (2) | CN101550410A (es) |
AR (2) | AR029759A1 (es) |
AU (1) | AU5805700A (es) |
CA (1) | CA2374009A1 (es) |
WO (1) | WO2001004273A2 (es) |
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US5665585A (en) * | 1992-09-03 | 1997-09-09 | Alko-Yhiot Oy | Recombinant production of glucoamylase P in trichoderma |
US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
DE4422198C2 (de) | 1994-06-24 | 1997-08-28 | Audi Ag | Verfahren zum Steuern der elektrischen Beheizung eines Katalysators |
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AU3892397A (en) * | 1996-07-24 | 1998-02-10 | Iowa State University Research Foundation Inc. | Protein engineering of glucoamylase to increase ph optimum, substrate specificity and thermostability |
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US6329186B1 (en) | 1998-12-07 | 2001-12-11 | Novozymes A/S | Glucoamylases with N-terminal extensions |
KR20010089885A (ko) * | 1998-12-07 | 2001-10-12 | 피아 스타르 | N-말단 연장을 가진 글루코아밀라제 |
AU5805700A (en) * | 1999-07-09 | 2001-01-30 | Novozymes A/S | Glucoamylase variant |
DE60142415D1 (de) * | 2000-04-28 | 2010-07-29 | Novozymes As | Variante eines lipolytischen Enzyms |
CN1875097B (zh) | 2003-10-28 | 2013-04-24 | 诺维信北美公司 | 杂交酶 |
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-
2000
- 2000-07-07 AU AU58057/00A patent/AU5805700A/en not_active Abandoned
- 2000-07-07 AR ARP000103471A patent/AR029759A1/es active IP Right Grant
- 2000-07-07 CN CNA2009101412582A patent/CN101550410A/zh active Pending
- 2000-07-07 CN CNB008101329A patent/CN100510067C/zh not_active Expired - Fee Related
- 2000-07-07 WO PCT/DK2000/000373 patent/WO2001004273A2/en active Application Filing
- 2000-07-07 CA CA002374009A patent/CA2374009A1/en not_active Abandoned
- 2000-07-07 JP JP2001509477A patent/JP2003504046A/ja active Pending
- 2000-07-07 EP EP00943688A patent/EP1200566A2/en not_active Ceased
- 2000-07-07 EP EP08159171A patent/EP2009098A1/en not_active Withdrawn
-
2003
- 2003-04-23 US US10/421,586 patent/US7354753B2/en not_active Expired - Fee Related
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2008
- 2008-02-21 US US12/034,906 patent/US7927857B2/en not_active Expired - Fee Related
- 2008-12-11 AR ARP080105383A patent/AR069653A2/es unknown
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2011
- 2011-03-01 US US13/037,751 patent/US8722369B2/en not_active Expired - Fee Related
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US20080213843A1 (en) | 2008-09-04 |
CN101550410A (zh) | 2009-10-07 |
AR029759A1 (es) | 2003-07-16 |
US20120208243A1 (en) | 2012-08-16 |
EP1200566A2 (en) | 2002-05-02 |
EP2009098A1 (en) | 2008-12-31 |
AU5805700A (en) | 2001-01-30 |
US20040002142A1 (en) | 2004-01-01 |
JP2003504046A (ja) | 2003-02-04 |
US7354753B2 (en) | 2008-04-08 |
CN100510067C (zh) | 2009-07-08 |
US8722369B2 (en) | 2014-05-13 |
US7927857B2 (en) | 2011-04-19 |
WO2001004273A3 (en) | 2001-09-07 |
CN1360630A (zh) | 2002-07-24 |
WO2001004273A2 (en) | 2001-01-18 |
CA2374009A1 (en) | 2001-01-18 |
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