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MXPA05005786A - Placas de electroporacion a gran escala, sistemas y metodos de uso. - Google Patents

Placas de electroporacion a gran escala, sistemas y metodos de uso.

Info

Publication number
MXPA05005786A
MXPA05005786A MXPA05005786A MXPA05005786A MXPA05005786A MX PA05005786 A MXPA05005786 A MX PA05005786A MX PA05005786 A MXPA05005786 A MX PA05005786A MX PA05005786 A MXPA05005786 A MX PA05005786A MX PA05005786 A MXPA05005786 A MX PA05005786A
Authority
MX
Mexico
Prior art keywords
electroporation
systems
plates
methods
electroporation plates
Prior art date
Application number
MXPA05005786A
Other languages
English (en)
Inventor
Andre S Gamelin
Original Assignee
Genetronics Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genetronics Inc filed Critical Genetronics Inc
Publication of MXPA05005786A publication Critical patent/MXPA05005786A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C12M23/12—Well or multiwell plates
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Computer Hardware Design (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Plant Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Peptides Or Proteins (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

La presente invencion describe placas de electroporacion (2) que comprenden una pluralidad de cavidades o camaras de electroporacion que se pueden proveer con energia (4), arregladas en un substrato solido, en donde al menos dos de las cavidades (4) en la placa (2) pueden ser provistas de energia independientemente. Cada cavidad (4) contiene al menos dos electrodos de electroporacion (12, 14) colocados en la misma, y sirven como el recipiente en el cual una reaccion de electroporacion individual puede ser llevada a cabo. Tambien se describen sistemas de electroporacion que utilizan placas de electroporacion (2) de acuerdo con la invencion, asi como metodos de uso de tales placas de electroporacion (2) y sistemas, por ejemplo, para optimizar las condiciones de electroporacion.
MXPA05005786A 2002-12-03 2003-12-03 Placas de electroporacion a gran escala, sistemas y metodos de uso. MXPA05005786A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US43073802P 2002-12-03 2002-12-03
PCT/US2003/038470 WO2004050866A1 (en) 2002-12-03 2003-12-03 Large-scale electroporation plates, systems, and methods of use

Publications (1)

Publication Number Publication Date
MXPA05005786A true MXPA05005786A (es) 2006-03-08

Family

ID=32469520

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA05005786A MXPA05005786A (es) 2002-12-03 2003-12-03 Placas de electroporacion a gran escala, sistemas y metodos de uso.

Country Status (11)

Country Link
US (1) US20060115888A1 (es)
EP (1) EP1578966A4 (es)
JP (1) JP2006508663A (es)
KR (1) KR20050084133A (es)
CN (1) CN1732260A (es)
AU (1) AU2003302502A1 (es)
CA (1) CA2508358A1 (es)
EA (1) EA009405B1 (es)
MX (1) MXPA05005786A (es)
NZ (1) NZ540442A (es)
WO (1) WO2004050866A1 (es)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7579734B2 (en) * 2003-03-17 2009-08-25 Panaphase Technologies, Llc Apparatus and method that prevent flux reversal in the stator back material of a two-phase SRM (TPSRM)
DE502004009820D1 (de) * 2004-03-15 2009-09-10 Lonza Cologne Ag Behältnis und Vorrichtung zur Erzeugung von elektrischen Feldern in einzelnen Reaktionsräumen
US8101169B2 (en) * 2005-02-23 2012-01-24 The Board Of Trustees Of The Leland Stanford Junior University Ocular gene therapy using avalanche-mediated transfection
US7923251B2 (en) * 2005-02-23 2011-04-12 The Board Of Trustees Of The Leland Stanford Junior University Method and apparatus for avalanche-mediated transfer of agents into cells
US7923238B2 (en) * 2006-02-10 2011-04-12 Bio-Rad Laboratories, Inc. Multi-channel electroporation system
US7799555B2 (en) 2006-02-10 2010-09-21 Bio-Rad Laboratories, Inc. Apparatus for high-throughput electroporation
US7943367B2 (en) * 2006-09-20 2011-05-17 Bio-Rad Laboratories, Inc. High-throughput electroporation chamber with functional lid for risk reduction
US7576549B2 (en) 2006-09-21 2009-08-18 Bio-Rad Laboratories, Inc. Methods for measuring sample resistance in electroporation
US7750605B2 (en) 2006-09-21 2010-07-06 Bio-Rad Laboratories, Inc. Controlling an electrical signal sent to a sample load using a pulse modulated resistance
US8017381B2 (en) 2007-03-08 2011-09-13 Bio-Rad Laboratories, Inc. Composite electroporation plate with interchangeable well inserts
US20120244593A1 (en) * 2009-10-19 2012-09-27 Yong Huang Method, device and apparatus for inducing self-adjusting cell electroporation
WO2011142813A1 (en) * 2010-05-12 2011-11-17 Cellectis Sa Dynamic mixing and electroporation chamber and system
EP2918667B1 (en) * 2012-12-12 2017-11-01 National University Corporation Toyohashi University of Technology Foreign substance introduction device and method of producing cells with introduced foreign substance
EP3260163B1 (en) * 2014-05-02 2019-07-17 Lonza Cologne GmbH Device and method for large volume transfection
WO2016003485A1 (en) 2014-07-03 2016-01-07 Massachusetts Institute Of Technology Microfluidic assay for rapid optimization of cell electroporation
CN106715672A (zh) 2014-09-30 2017-05-24 国立研究开发法人科学技术振兴机构 气泡喷出头、局部消融装置及局部消融方法、以及注射装置及注射方法
US11054408B2 (en) * 2016-05-06 2021-07-06 StemoniX Inc. Projected capacitive multi electrode eukaryotic cell array
US10233419B2 (en) * 2016-06-30 2019-03-19 Zymergen Inc. Apparatuses and methods for electroporation
ES2933512T3 (es) * 2017-08-16 2023-02-09 Amgen Inc Disposición de electrodos adaptable para su implementación en un sistema de incubación de células y sus aplicaciones
WO2019059936A1 (en) * 2017-09-23 2019-03-28 Hewlett-Packard Development Company, L.P. ELECTROPORATION
CN114196532A (zh) * 2021-11-01 2022-03-18 上海盟德生物科技有限公司 一种多通道的细胞电转仪
CN117660703A (zh) * 2022-08-25 2024-03-08 苏州壹达生物科技有限公司 一种流式电穿孔装置及其控制方法

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5371003A (en) * 1987-05-05 1994-12-06 Sandoz Ltd. Electrotransformation process
US5128257A (en) * 1987-08-31 1992-07-07 Baer Bradford W Electroporation apparatus and process
JP2829005B2 (ja) * 1988-11-11 1998-11-25 株式会社日立製作所 マイクロチャンバープレート、これを利用した細胞検出方法、処理方法および装置ならびに細胞
US5183744A (en) * 1988-10-26 1993-02-02 Hitachi, Ltd. Cell handling method for cell fusion processor
DE59108591D1 (de) * 1991-12-06 1997-04-10 Ciba Geigy Ag Elektrophoretische Trennvorrichtung und elektrophoretisches Trennverfahren
US5545130A (en) * 1992-04-08 1996-08-13 Genetronics, Inc. Flow through electroporation method
US6284459B1 (en) * 1995-04-25 2001-09-04 Discovery Partners International Solid support matrices with memories and combinatorial libraries therefrom
US6416959B1 (en) * 1997-02-27 2002-07-09 Kenneth Giuliano System for cell-based screening
US6548263B1 (en) * 1997-05-29 2003-04-15 Cellomics, Inc. Miniaturized cell array methods and apparatus for cell-based screening
KR100247935B1 (ko) * 1997-10-22 2000-03-15 윤종용 오산화 이탄탈륨 유전체막을 갖는 커패시터 형성방법
WO2000034434A1 (en) * 1998-12-07 2000-06-15 Acacia Biosciences, Inc. Multi-channel electrode arrays
DE19917571B4 (de) * 1999-04-19 2014-03-20 Robert Poppe Elektrodenraster für Elektroporationsreaktionsansätze
US6762049B2 (en) * 2001-07-05 2004-07-13 Institute Of Microelectronics Miniaturized multi-chamber thermal cycler for independent thermal multiplexing

Also Published As

Publication number Publication date
EA200500911A1 (ru) 2005-12-29
CN1732260A (zh) 2006-02-08
AU2003302502A1 (en) 2004-06-23
CA2508358A1 (en) 2004-06-17
JP2006508663A (ja) 2006-03-16
NZ540442A (en) 2007-05-31
EP1578966A1 (en) 2005-09-28
US20060115888A1 (en) 2006-06-01
WO2004050866A1 (en) 2004-06-17
KR20050084133A (ko) 2005-08-26
EA009405B1 (ru) 2007-12-28
EP1578966A4 (en) 2007-09-05

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