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MX2021001619A - Microorganismos con produccion mejorada de 1,3-propanodiol y acido butirico. - Google Patents

Microorganismos con produccion mejorada de 1,3-propanodiol y acido butirico.

Info

Publication number
MX2021001619A
MX2021001619A MX2021001619A MX2021001619A MX2021001619A MX 2021001619 A MX2021001619 A MX 2021001619A MX 2021001619 A MX2021001619 A MX 2021001619A MX 2021001619 A MX2021001619 A MX 2021001619A MX 2021001619 A MX2021001619 A MX 2021001619A
Authority
MX
Mexico
Prior art keywords
propanediol
microorganisms
improved
butyric acid
clostridium
Prior art date
Application number
MX2021001619A
Other languages
English (en)
Inventor
Laurence Dumon-Seignovert
Céline Raynaud
Wanda Dischert
Nadège Dumoulin
Original Assignee
Metabolic Explorer Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Metabolic Explorer Sa filed Critical Metabolic Explorer Sa
Publication of MX2021001619A publication Critical patent/MX2021001619A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/52—Propionic acid; Butyric acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/33—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Clostridium (G)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0067—Oxidoreductases (1.) acting on hydrogen as donor (1.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y112/00—Oxidoreductases acting on hydrogen as donor (1.12)
    • C12Y112/07—Oxidoreductases acting on hydrogen as donor (1.12) with an iron-sulfur protein as acceptor (1.12.7)
    • C12Y112/07002—Ferredoxin hydrogenase (1.12.7.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/0103—Glycerol kinase (2.7.1.30)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

La presente invención se refiere a una nueva cepa mutante de Clostridium acetobutylicum que comprende una actividad atenuada de glicerol quinasa; además, la presente invención se refiere a un consorcio de Clostridium que comprende al menos dicha cepa mutante, y al menos otra especie de Clostridia elegida entre C. sporogenes y C. sphenoides; como esta cepa modificada puede adaptarse para el crecimiento y la producción de 1,3-propanodiol en un medio de cultivo apropiado con alto contenido de glicerol, la invención también se refiere a un método para la producción de 1,3-propanodiol y ácido butírico, al cultivar al menos esta cepa mutante en un medio de cultivo apropiado.
MX2021001619A 2018-08-10 2019-08-09 Microorganismos con produccion mejorada de 1,3-propanodiol y acido butirico. MX2021001619A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP18306099 2018-08-10
PCT/EP2019/071392 WO2020030775A1 (en) 2018-08-10 2019-08-09 Microorganisms with improved 1,3-propanediol and butyric acid production

Publications (1)

Publication Number Publication Date
MX2021001619A true MX2021001619A (es) 2021-05-12

Family

ID=63449410

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021001619A MX2021001619A (es) 2018-08-10 2019-08-09 Microorganismos con produccion mejorada de 1,3-propanodiol y acido butirico.

Country Status (13)

Country Link
US (1) US11814663B2 (es)
EP (1) EP3833770A1 (es)
JP (1) JP7569783B2 (es)
KR (1) KR20210043606A (es)
CN (1) CN112567044A (es)
AR (1) AR114547A1 (es)
AU (1) AU2019319045A1 (es)
BR (1) BR112021002573A2 (es)
CA (1) CA3107573A1 (es)
IL (1) IL280749A (es)
MX (1) MX2021001619A (es)
SG (1) SG11202101283WA (es)
WO (1) WO2020030775A1 (es)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IT202000031979A1 (it) 2020-12-22 2022-06-22 Novamont Spa Processo di purificazione di una miscela comprendente dioli e acetali
IT202100006419A1 (it) 2021-03-17 2022-09-17 Novamont Spa Recupero di dioli da una miscela

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2633939B1 (fr) 1988-07-06 1991-05-31 Inst Francais Du Petrole Procede de production d'acide butyrique normal par fermentation a partir d'un substrat sucre en presence d'au moins une souche de l'espece clostridium
FR2796081B1 (fr) 1999-07-09 2003-09-26 Agronomique Inst Nat Rech Procede de preparation du 1,3-propanediol par un micro-organisme recombinant en l'absence de coenzyme b12 ou de l'un de ses precurseurs
AU2002306849A1 (en) * 2001-03-21 2002-10-08 Elitra Pharmaceuticals, Inc. Identification of essential genes in microorganisms
KR101148255B1 (ko) 2002-10-04 2012-08-08 다니스코 유에스 인크. 고수율을 갖는 1,3-프로판디올의 생물학적 제조 방법
CN1327001C (zh) 2005-06-03 2007-07-18 清华大学 利用生物柴油副产物甘油生产1,3－丙二醇的方法
JP5203375B2 (ja) 2006-10-03 2013-06-05 メタボリック エクスプローラー クロストリジウム菌における染色体取り込みおよびｄｎａ配列置換法
US8236994B2 (en) * 2006-10-31 2012-08-07 Metabolic Explorer Process for the biological production of 1,3-propanediol from glycerol with high yield
BRPI0814640A2 (pt) * 2007-07-23 2014-10-14 Dsm Ip Assets Bv Processo para a preparação de butanol e etanol
ES2388754T3 (es) 2007-11-30 2012-10-18 Metabolic Explorer Procedimiento para la purificación de un alcohol a partir de un caldo de fermentación
CN102238990B (zh) 2008-10-03 2014-07-23 代谢探索者公司 使用降膜、刮膜、薄膜或短程蒸发器从发酵液纯化醇的方法
EP2248904A1 (en) 2009-05-05 2010-11-10 Metabolic Explorer Continuous culture for 1,3-propanediol production using high glycerine concentration
AR078525A1 (es) 2009-10-05 2011-11-16 Metabolic Explorer Sa Produccion de 1,3-propanodiol con reactores de celulas inmovilizadas
AR083799A1 (es) * 2010-11-10 2013-03-20 Metabolic Explorer Sa Microorganismos para la produccion de 1,3-propanodiol usando concentracion de glicerina alta
GB201116971D0 (en) * 2011-10-03 2011-11-16 Isis Innovation Cofactor regeneration system
KR20140032057A (ko) * 2012-09-05 2014-03-14 삼성전자주식회사 글리세롤 탈수 반응 산물의 생산 능력이 향상된 재조합 미생물 및 그 용도
PT3194568T (pt) * 2014-09-18 2019-09-16 Institute National De La Rech Agronomique Estirpes de clostridium acetobutylicum incapazes de produzir hidrogénio e úteis para a produção contínua de produtos químicos e combustíveis
FR3039167B1 (fr) 2015-07-23 2023-11-10 Afyren Procede d'extraction de metabolites fermentaires produits par fermentation anaerobie a partir de biomasse fermentescible
PT3583221T (pt) * 2017-02-20 2021-09-24 Metabolic Explorer Sa Consórcio microbiano compreendendo clostridia para a produção de 1,3-propanodiol a partir de glicerol

Also Published As

Publication number Publication date
AR114547A1 (es) 2020-09-16
BR112021002573A2 (pt) 2021-05-11
IL280749A (en) 2021-04-29
US20210254108A1 (en) 2021-08-19
JP2021533764A (ja) 2021-12-09
SG11202101283WA (en) 2021-03-30
US11814663B2 (en) 2023-11-14
CN112567044A (zh) 2021-03-26
EP3833770A1 (en) 2021-06-16
KR20210043606A (ko) 2021-04-21
AU2019319045A1 (en) 2021-02-18
CA3107573A1 (en) 2020-02-13
WO2020030775A1 (en) 2020-02-13
JP7569783B2 (ja) 2024-10-18

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