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MX2021000946A - Metodo de deteccion de acido nucleico. - Google Patents

Metodo de deteccion de acido nucleico.

Info

Publication number
MX2021000946A
MX2021000946A MX2021000946A MX2021000946A MX2021000946A MX 2021000946 A MX2021000946 A MX 2021000946A MX 2021000946 A MX2021000946 A MX 2021000946A MX 2021000946 A MX2021000946 A MX 2021000946A MX 2021000946 A MX2021000946 A MX 2021000946A
Authority
MX
Mexico
Prior art keywords
nucleic acid
detection method
acid detection
methods
produce
Prior art date
Application number
MX2021000946A
Other languages
English (en)
Inventor
Henry John Lamble
David Lloyd
Original Assignee
Sense Biodetection Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Sense Biodetection Ltd filed Critical Sense Biodetection Ltd
Publication of MX2021000946A publication Critical patent/MX2021000946A/es

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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683—Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
    • C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54306—Solid-phase reaction mechanisms
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    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/10—Nucleotidyl transfering
    • C12Q2521/101—DNA polymerase
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    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/101—Modifications characterised by incorporating non-naturally occurring nucleotides, e.g. inosine
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    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/119—Strand displacement amplification [SDA]
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    • C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
    • C12Q2563/107—Nucleic acid detection characterized by the use of physical, structural and functional properties fluorescence
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    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/178—Oligonucleotides characterized by their use miRNA, siRNA or ncRNA

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

La presente invención se refiere a métodos para la detección de ácidos nucleicos de secuencia definida y a kits y a dispositivos para uso en dichos métodos; los métodos emplean enzimas de restricción, polimerasa y cebadores de oligonucleótidos para producir un producto de amplificación en la presencia de un ácido nucleico diana, que se pone en contacto con sondas de oligonucleótidos para producir un producto detector.
MX2021000946A 2018-07-25 2019-07-25 Metodo de deteccion de acido nucleico. MX2021000946A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1812149.1A GB201812149D0 (en) 2018-07-25 2018-07-25 Nucleic acid detection method
PCT/GB2019/052089 WO2020021272A1 (en) 2018-07-25 2019-07-25 Nucleic acid detection method

Publications (1)

Publication Number Publication Date
MX2021000946A true MX2021000946A (es) 2021-03-31

Family

ID=63364368

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2021000946A MX2021000946A (es) 2018-07-25 2019-07-25 Metodo de deteccion de acido nucleico.

Country Status (12)

Country Link
US (2) US20210246487A1 (es)
EP (1) EP3784799A1 (es)
JP (1) JP2021531778A (es)
KR (1) KR20210038541A (es)
CN (1) CN112469833A (es)
AU (2) AU2019311340B2 (es)
BR (1) BR112021000574A2 (es)
CA (1) CA3107388A1 (es)
GB (2) GB201812149D0 (es)
MX (1) MX2021000946A (es)
WO (1) WO2020021272A1 (es)
ZA (1) ZA202007778B (es)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
GB2569965A (en) 2018-01-04 2019-07-10 Lumiradx Uk Ltd Improvements in or relating to amplification of nucleic acids
US20210040571A1 (en) * 2018-07-25 2021-02-11 Sense Biodetection Limited Nucleic acid detection method
AU2021333751A1 (en) 2020-08-26 2023-04-27 Sense Biodetection Limited Resilient biased latch with fusible retaining member
JP2023539297A (ja) 2020-08-26 2023-09-13 センス バイオディテクション リミテッド デバイス
CN113624980B (zh) * 2021-08-09 2023-06-13 四川大学华西医院 基于识别诱导的恒温扩增技术对蛋白质进行检测的方法及试剂盒
CN114107510B (zh) * 2021-12-10 2023-10-03 湖南工程学院 基于dna三链介导构建多维dna酶矩阵的超灵敏循环核酸检测体系、试剂盒和方法
CN117568446A (zh) * 2022-08-08 2024-02-20 台达电子国际（新加坡）私人有限公司 检测病毒感染性的样本前处理试剂盒及方法
WO2024072805A1 (en) * 2022-09-26 2024-04-04 Lau, Johnson Yiu-Nam Compositions, systems, and methods for detection of ovarian cancer
WO2024080776A1 (ko) * 2022-10-14 2024-04-18 고려대학교 산학협력단 비색 검출을 위한 황 함유 프로브 및 핵산 내 황 도입을 통한 효소 활성 가속화 방법
CN116536400A (zh) * 2023-04-06 2023-08-04 广州迪澳基因科技有限公司 一种高特异性等温核酸扩增方法
CN117046441B (zh) * 2023-08-14 2024-04-02 中国科学院生态环境研究中心 磁性氧化石墨烯粒子、制备方法及其应用

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US5200313A (en) * 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
US5455166A (en) * 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US6531302B1 (en) * 1999-04-12 2003-03-11 Nanogen/Becton Dickinson Partnership Anchored strand displacement amplification on an electronically addressable microchip
US7364848B2 (en) * 2002-09-02 2008-04-29 Pamgene B.V. Integrated microarray analysis
US9689031B2 (en) 2007-07-14 2017-06-27 Ionian Technologies, Inc. Nicking and extension amplification reaction for the exponential amplification of nucleic acids
US20100279295A1 (en) * 2009-03-18 2010-11-04 Sequenom, Inc. Use of thermostable endonucleases for generating reporter molecules
KR102362649B1 (ko) * 2012-04-09 2022-02-14 엔바이로로직스 인크. 시료 내 핵산 서열을 정량화하기 위한 조성물 및 방법
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US10337072B2 (en) * 2015-01-08 2019-07-02 The Board Of Trustees Of The University Of Illinois Copy number detection and methods
GB201611469D0 (en) 2016-06-30 2016-08-17 Lumiradx Tech Ltd Improvements in or relating to nucleic acid amplification processes
US20190376126A1 (en) * 2016-12-23 2019-12-12 Albert-Ludwigs-Universität Freiburg Two-part mediator probe

Also Published As

Publication number Publication date
US20210024998A1 (en) 2021-01-28
AU2023201422A1 (en) 2023-04-06
GB201812149D0 (en) 2018-09-05
CN112469833A (zh) 2021-03-09
GB2577171B (en) 2021-08-11
AU2019311340B2 (en) 2023-03-30
WO2020021272A1 (en) 2020-01-30
JP2021531778A (ja) 2021-11-25
BR112021000574A2 (pt) 2021-04-06
AU2019311340A1 (en) 2021-01-07
KR20210038541A (ko) 2021-04-07
GB201910661D0 (en) 2019-09-11
US20210246487A1 (en) 2021-08-12
CA3107388A1 (en) 2020-01-30
ZA202007778B (en) 2022-03-30
EP3784799A1 (en) 2021-03-03
GB2577171A (en) 2020-03-18

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