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MX2019006628A - Purificacion y medicion integradas de metilacion de adn y co-medicion de mutaciones y/o niveles de expresion de arnm en un cartucho de reaccion automatizado. - Google Patents

Purificacion y medicion integradas de metilacion de adn y co-medicion de mutaciones y/o niveles de expresion de arnm en un cartucho de reaccion automatizado.

Info

Publication number
MX2019006628A
MX2019006628A MX2019006628A MX2019006628A MX2019006628A MX 2019006628 A MX2019006628 A MX 2019006628A MX 2019006628 A MX2019006628 A MX 2019006628A MX 2019006628 A MX2019006628 A MX 2019006628A MX 2019006628 A MX2019006628 A MX 2019006628A
Authority
MX
Mexico
Prior art keywords
nucleic acid
dna
matrix material
measurement
deaminated
Prior art date
Application number
MX2019006628A
Other languages
English (en)
Inventor
Wei- Lung Lai Edwin
Kohlway Andrew
Van Atta Reuel
Higuchi Russell
A Gall Alexander
Kocmond Kriszten
Original Assignee
Cepheid
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cepheid filed Critical Cepheid
Publication of MX2019006628A publication Critical patent/MX2019006628A/es

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    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L7/00—Heating or cooling apparatus; Heat insulating devices
    • B01L7/52—Heating or cooling apparatus; Heat insulating devices with provision for submitting samples to a predetermined sequence of different temperatures, e.g. for treating nucleic acid samples
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
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    • C12Q1/6844—Nucleic acid amplification reactions
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    • B01L2300/18—Means for temperature control
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    • B01L2400/0644—Valves, specific forms thereof with moving parts rotary valves
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    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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    • C12Q2531/00—Reactions of nucleic acids characterised by
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    • C12Q2535/00—Reactions characterised by the assay type for determining the identity of a nucleotide base or a sequence of oligonucleotides
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/164—Methylation detection other then bisulfite or methylation sensitive restriction endonucleases

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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
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Abstract

Se proporcionan métodos para determinar la metilación de ADN. En una modalidad ilustrativa, pero no limitante, el método comprende i) poner en contacto una muestra biológica que comprende un ácido nucleico con un primer material de matriz que comprende una primera columna o filtro donde el material de matriz se une y/o filtra ácidos nucleicos en la muestra y por lo tanto, purifica el ADN; ii) eluir el ADN unido del primer material de la matriz y desnaturalizar el ADN para producir ADN desnaturalizado eluido; iii) calentar el ADN eluido en presencia de iones bisulfito para producir un ácido nucleico desaminado; iv) poner en contacto el ácido nucleico desaminado con un segundo material de matriz que comprende una segunda columna para unir este ácido nucleico desaminado con este segundo material de matriz; v) desulfonar el ácido nucleico desaminado unido y/o simultáneamente eluir y desulfonar el ácido nucleico poniendo en contacto el ácido nucleico desaminado con una solución alcalina para producir un ácido nucleico convertido con bisulfito; vi) eluir este ácido nucleico convertido con bisulfito del segundo material de matriz; y vii) realizar la PCR específica de metilación y/o secuenciación de ácido nucleico, y/o análisis de fusión de alta resolución (HRM) en el ácido nucleico convertido con bisulfito para determinar la metilación del ácido nucleico, en donde al menos los pasos iv) a vi) se realizan en un solo cartucho de reacción.
MX2019006628A 2016-12-12 2017-12-12 Purificacion y medicion integradas de metilacion de adn y co-medicion de mutaciones y/o niveles de expresion de arnm en un cartucho de reaccion automatizado. MX2019006628A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201662433165P 2016-12-12 2016-12-12
PCT/US2017/065905 WO2018111935A1 (en) 2016-12-12 2017-12-12 Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge

Publications (1)

Publication Number Publication Date
MX2019006628A true MX2019006628A (es) 2019-11-12

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MX2019006628A MX2019006628A (es) 2016-12-12 2017-12-12 Purificacion y medicion integradas de metilacion de adn y co-medicion de mutaciones y/o niveles de expresion de arnm en un cartucho de reaccion automatizado.

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US (2) US11260387B2 (es)
EP (1) EP3551332B1 (es)
JP (1) JP7123050B2 (es)
KR (1) KR102479432B1 (es)
CN (1) CN110267744A (es)
AU (1) AU2017376118B2 (es)
CA (1) CA3046636A1 (es)
MX (1) MX2019006628A (es)
WO (1) WO2018111935A1 (es)
ZA (1) ZA201903750B (es)

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CA2989573A1 (en) 2015-06-15 2016-12-22 Cepheid Integrated purification and measurement of dna methylation and co-measurement of mutations and/or mrna expression levels in an automated reaction cartridge
MX2019006628A (es) 2016-12-12 2019-11-12 Cepheid Purificacion y medicion integradas de metilacion de adn y co-medicion de mutaciones y/o niveles de expresion de arnm en un cartucho de reaccion automatizado.
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CN112176419B (zh) * 2019-10-16 2022-03-22 中国医学科学院肿瘤医院 一种检测ctDNA中肿瘤特异基因的变异和甲基化的方法
CN111500731A (zh) * 2020-05-20 2020-08-07 深圳市新合生物医疗科技有限公司 一种甲状腺癌早期诊断、检测或者筛查的试剂盒及其使用方法、应用
CN111662980A (zh) * 2020-06-03 2020-09-15 广州市康立明生物科技有限责任公司 一种肺癌检测试剂及试剂盒
CN111705130B (zh) * 2020-06-03 2022-07-12 广州康立明生物科技股份有限公司 一种基因标志物组合及其应用
WO2021254518A1 (zh) * 2020-06-19 2021-12-23 中国疾病预防控制中心病毒病预防控制所 一种样品处理及检测装置
CN113832141B (zh) * 2021-09-30 2024-03-08 苏州唯善生物科技有限公司 粪便人源基因组dna特异性纯化方法及试剂盒
CN113699242A (zh) * 2021-10-18 2021-11-26 浙江省人民医院 检测kras基因突变、adamts1与bnc1甲基化的引物探针，试剂盒与方法
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