[go: up one dir, main page]
More Web Proxy on the site http://driver.im/

MX2016012206A - Variantes de t7 rna polimerasa con rango de substrato expandido y rendimiento de transcripcion mejorado. - Google Patents

Variantes de t7 rna polimerasa con rango de substrato expandido y rendimiento de transcripcion mejorado.

Info

Publication number
MX2016012206A
MX2016012206A MX2016012206A MX2016012206A MX2016012206A MX 2016012206 A MX2016012206 A MX 2016012206A MX 2016012206 A MX2016012206 A MX 2016012206A MX 2016012206 A MX2016012206 A MX 2016012206A MX 2016012206 A MX2016012206 A MX 2016012206A
Authority
MX
Mexico
Prior art keywords
rna polymerase
variants
substrate range
polymerase variants
expanded substrate
Prior art date
Application number
MX2016012206A
Other languages
English (en)
Inventor
D Ellington Andrew
J Meyer Adam
Original Assignee
Univ Texas
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Texas filed Critical Univ Texas
Publication of MX2016012206A publication Critical patent/MX2016012206A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1241—Nucleotidyltransferases (2.7.7)
    • C12N9/1247—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/111—General methods applicable to biologically active non-coding nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/07—Nucleotidyltransferases (2.7.7)
    • C12Y207/07006—DNA-directed RNA polymerase (2.7.7.6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/14—Type of nucleic acid interfering N.A.
    • C12N2310/141—MicroRNAs, miRNAs
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/16—Aptamers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2320/00—Applications; Uses
    • C12N2320/50—Methods for regulating/modulating their activity
    • C12N2320/51—Methods for regulating/modulating their activity modulating the chemical stability, e.g. nuclease-resistance
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2330/00—Production
    • C12N2330/50—Biochemical production, i.e. in a transformed host cell
    • C12N2330/51—Specially adapted vectors

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Se describen variantes de T7 RNA polimerasa con actividad de transcripción mejorada. Las variantes de T7 RNA polimerasa son conocidas, las cuales tienen la capacidad de incorporar ribonucleótidos modificados en moléculas de RNA crecientes. Sin embargo, estas variantes tienen niveles relativamente bajos de actividad de transcripción. Se presentan en la presente mutaciones que aumentan la actividad de transcripción de las variantes con amplio rango de substratos.
MX2016012206A 2014-03-20 2015-03-20 Variantes de t7 rna polimerasa con rango de substrato expandido y rendimiento de transcripcion mejorado. MX2016012206A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201461968231P 2014-03-20 2014-03-20
PCT/US2015/021748 WO2015143318A1 (en) 2014-03-20 2015-03-20 T7 rna polymerase variants with expanded substrate range and enhanced transcriptional yield

Publications (1)

Publication Number Publication Date
MX2016012206A true MX2016012206A (es) 2017-04-06

Family

ID=54145383

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2016012206A MX2016012206A (es) 2014-03-20 2015-03-20 Variantes de t7 rna polimerasa con rango de substrato expandido y rendimiento de transcripcion mejorado.

Country Status (12)

Country Link
US (1) US9988612B2 (es)
EP (1) EP3119873A4 (es)
JP (1) JP2017509341A (es)
KR (1) KR20160132113A (es)
CN (1) CN106459879A (es)
AU (1) AU2015231031A1 (es)
BR (1) BR112016021627A2 (es)
CA (1) CA2942513A1 (es)
IL (1) IL247864A0 (es)
MX (1) MX2016012206A (es)
SG (1) SG11201607720XA (es)
WO (1) WO2015143318A1 (es)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10034951B1 (en) * 2017-06-21 2018-07-31 New England Biolabs, Inc. Use of thermostable RNA polymerases to produce RNAs having reduced immunogenicity
EP3645712A4 (en) 2017-06-30 2021-07-07 Codexis, Inc. T7 RNA POLYMERASE VARIANTS
JP2020530266A (ja) * 2017-06-30 2020-10-22 コデクシス， インコーポレイテッド Ｔ７ ｒｎａポリメラーゼバリアント
EP4397757A3 (en) * 2017-08-18 2024-10-23 ModernaTX, Inc. Rna polymerase variants
AU2019253693A1 (en) * 2018-04-10 2020-10-22 Greenlight Biosciences, Inc. T7 RNA polymerase variants
WO2020056111A1 (en) * 2018-09-12 2020-03-19 Guardian Therapeutics, Llc Rna polymerase for synthesis of modified rna
WO2021228905A2 (en) * 2020-05-11 2021-11-18 Thermo Fisher Scientific Baltics Uab Mutant polymerases and methods of using the same

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001066705A1 (en) * 2000-03-07 2001-09-13 Akzo Nobel N.V. Rna polymerase mutants with increased thermostability
JP2005346791A (ja) 2004-06-01 2005-12-15 Tdk Corp 光記録媒体及びその製造方法
CN101495504A (zh) 2005-06-30 2009-07-29 阿切埃米克斯有限公司 用于生产全2’-修饰的核酸转录产物的材料和方法
AU2007337810B2 (en) * 2006-12-22 2014-02-13 Archemix Corp. Materials and methods for the generation of transcripts comprising modified nucleotides
JP5625908B2 (ja) * 2008-08-08 2014-11-19 東ソー株式会社 機能改善したｒｎａポリメラーゼ変異体
US9062292B2 (en) * 2010-09-13 2015-06-23 Enzo Life Sciences Inc. Mutant T7 polymerases
WO2014028429A2 (en) * 2012-08-14 2014-02-20 Moderna Therapeutics, Inc. Enzymes and polymerases for the synthesis of rna

Also Published As

Publication number Publication date
WO2015143318A1 (en) 2015-09-24
BR112016021627A2 (pt) 2018-07-10
EP3119873A4 (en) 2018-01-03
SG11201607720XA (en) 2016-10-28
US9988612B2 (en) 2018-06-05
JP2017509341A (ja) 2017-04-06
KR20160132113A (ko) 2016-11-16
IL247864A0 (en) 2016-11-30
EP3119873A1 (en) 2017-01-25
CA2942513A1 (en) 2015-09-24
AU2015231031A1 (en) 2016-09-22
CN106459879A (zh) 2017-02-22
US20170211050A1 (en) 2017-07-27

Similar Documents

Publication Publication Date Title
MX2016012206A (es) Variantes de t7 rna polimerasa con rango de substrato expandido y rendimiento de transcripcion mejorado.
IL273460A (en) Changing the specificity of plant non-coding RNA sequences to silence gene expression
EP3642342A4 (en) RNA MOLECULES, CIRCULAR RNA PRODUCTION METHODS, AND PROCESSING METHODS
MX2016007541A (es) Moléculas modificadas de ácido nucleico y usos de las mismas.
WO2014028429A3 (en) Enzymes and polymerases for the synthesis of rna
PH12016502564A1 (en) Substituted nucleosides, nucleosides and analogs thereof
PH12016502563A1 (en) Substituted nucleosides, nucleotides and analogs thereof
EP3286338A4 (en) Amplification with primers of limited nucleotide composition
WO2015196128A3 (en) Alternative nucleic acid molecules and uses thereof
WO2015196130A3 (en) Alternative nucleic acid molecules and uses thereof
MX2017008184A (es) Nucleosidos sustituidos, nucleotidos y analogos de estos.
EP3041948A4 (en) Alternative nucleic acid molecules containing reduced uracil content and uses thereof
EP3095460A4 (en) Chiral nucleic acid adjuvant having anti-allergic activity, and anti-allergic agent
BR112016028045A8 (pt) biopolímeros modificados, e seus métodos de produção
MX354267B (es) Nucléosidos, nucleótidos, y ácidos nucleicos modificados, y usos de los mismos.
EP3294450A4 (en) PROCESS FOR PRODUCING MODIFIED CLAY, MODIFIED CLAY PRODUCED AND USE THEREOF
EA039598B9 (ru) Соединение, нацеленное на ил-23а и фно-альфа, и его применение
EP3368654A4 (en) DETERMINATION OF POLYMORPHISMS BY ISOTHERMAL AMPLIFICATION OF NUCLEIC ACID
TW201613949A (en) Targeting microRNAs for metabolic disorders
HK1259060A1 (zh) 抑制腎素原基因或腎素原受體基因表達的單鏈核酸分子及其用途
MX2018005872A (es) Profarmacos de acido nucleico.
WO2016106387A3 (en) Nucleic acid aptamers to treat histone-induced disease states
EP3387431A4 (en) Systems, methods and compositions for improving the specificity of nucleic acid hybridization
EP3631006A4 (en) DNA STABILIZATION OF RNA
WO2016027263A3 (en) Stabilized thrombin