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Duffy et al., 2015 - Google Patents

Tracking distinct RNA populations using efficient and reversible covalent chemistry

Duffy et al., 2015

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Document ID
3744635703973477840
Author
Duffy E
Rutenberg-Schoenberg M
Stark C
Kitchen R
Gerstein M
Simon M
Publication year
Publication venue
Molecular cell

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Snippet

We describe a chemical method to label and purify 4-thiouridine (s 4 U)-containing RNA. We demonstrate that methanethiosulfonate (MTS) reagents form disulfide bonds with s 4 U more efficiently than the commonly used HPDP-biotin, leading to higher yields and less biased …
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or micro-organisms; Compositions therefor; Processes of preparing such compositions
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