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Bron et al., 2002 - Google Patents

Use of the alr gene as a food-grade selection marker in lactic acid bacteria

Bron et al., 2002

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Document ID
3601196839371173538
Author
Bron P
Benchimol M
Lambert J
Palumbo E
Deghorain M
Delcour J
De Vos W
Kleerebezem M
Hols P
Publication year
Publication venue
Applied and environmental microbiology

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ABSTRACT Both Lactococcus lactis and Lactobacillus plantarum contain a single alr gene, encoding an alanine racemase (EC 5.1. 1.1), which catalyzes the interconversion of d- alanine and l-alanine. The alr genes of these lactic acid bacteria were investigated for their …
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/746—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for lactic acid bacteria (Streptococcus; Lactococcus; Lactobacillus; Pediococcus; Enterococcus; Leuconostoc; Propionibacterium; Bifidobacterium; Sporolactobacillus)
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